<?xml version="1.0" encoding="UTF-8"?>
<uniprot xmlns="https://uniprot.org/uniprot"
 xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance"
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<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBC2</accession>
    <name>C5DBC2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0A01342p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0A01342g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551521.1">
        <property type="nucleotide sequence ID" value="XM_002551475.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DBC2">
        <property type="interactions" value="802"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBC2"/>
    <dbReference type="GeneID" id="8290313"/>
    <dbReference type="KEGG" id="lth:KLTH0A01342g"/>
    <dbReference type="eggNOG" id="KOG1848">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001169_1_0_1"/>
    <dbReference type="InParanoid" id="C5DBC2"/>
    <dbReference type="OMA" id="AWRLCLN"/>
    <dbReference type="OrthoDB" id="294853at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032629">
        <property type="entry name" value="DCB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032691">
        <property type="entry name" value="Mon2/Sec7/BIG1-like_HUS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032817">
        <property type="entry name" value="Mon2_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16213">
        <property type="entry name" value="DCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16206">
        <property type="entry name" value="Mon2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12783">
        <property type="entry name" value="Sec7-like_HUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Mon2/Sec7/BIG1-like dimerisation and cyclophilin-binding" evidence="5">
        <location>
            <begin position="7"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="Mon2/Sec7/BIG1-like HUS" evidence="3">
        <location>
            <begin position="200"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="Mon2 C-terminal" evidence="4">
        <location>
            <begin position="905"/>
            <end position="1134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF16213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR21079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1614" mass="183034" checksum="3F9498CE01F68D14" modified="2009-07-28" version="1">MSTKLSFRKFSDELFTELHALSAESKRRSSEVRHASDKSIEILKTVHEFEDLPRHPDFVTPFVLSCASKNAKLTSVSVQCLQKMSSVNCIPEDRIEDVLDAFIDSTHLAAEIQLKVLQIIPLFFKTYSQVVVGKLCSKLLLCCSSLIQIPNKAPVVFGTASATLQQLINDILERATIDQESRTCKVAVNNIEFIEVGSFRHDANRLFNDLCNLKNASYSPKDCILNVESIPEDYGLEVLESVLRNHRKLFAACPDLQFVLRTRAVPLLLRSISSSKSFPIVVRSARCLTLLIQEEFLAILELELEIILFLLINILSNEIESPIWKKIISLELFQLVCKNFALVWGIFKSYDVHPDRRQILKSLLSTLKGILSQKEYHEYLKVLHVLTRGDTPIVSQEYSVAKIPFIDLLDKASAPLVDQTYVVYLVLAVTNSISDGMGSQAVSLYQKQAKEEEVKSLKLMYSELFDDLFFIHKMFLYASTLDTPLFHSVVRAFQKLSHSAGVLECENKLNKCLRLFGIATVESCHIMGEAPEDNESDRSLTTASTVINTISESLIGNSSNAKGSFEANLQHSRAIHQRTISVFRALMSLSLSLGSVFSSDSWKEVLKTWQWMSYYLEGPSRDSDHSTHGFKTAQNPRASSSDISAAKFSIIKLYESTQHYSSSSFNVMVSSVIQQSKECAELITSNYAPEGHSPVDGSKMLNTCIFNRDFFIAQLRELSQINIWRLVNESKWRPSWDLVISYLVEVAASREIANEKLRLSAVDVFNNIISRAAIVSSDPEIPQKTDTEAAQSRLLTALMQMINSIMSLKRTRDDVRNGAVETEYDILFQVLRTLKGLLDIFGDSLQGAWDVVFKIINCPFVIMSESSITELSDHEEDSSILETISSKHNGVIKLSFEVFKLIFDDFLQTLPLDVVKNVIDTLLNFVRQDRDLNISFSSISQFWLIGDYLRTCVSKVPSEFTDSQKLAFAKKIEDGQLISMISDQNTEPCGLYYGLWLYLLKKLVECTNDERVEVKNGAIQTFFRIIDSHSSSFPPWELIVHEVIKPLLQQRSNFEEYLESGEYVNLTLKGLIHMFALYLAKFSHTSDFTEAWSLLVCYMADLVILPSFEISFIVLSNLRELLQAVLKIQDFPARITSEIYQRWCGYNVIYADPAKSSELKRKTNYDCVEELLLCYPFLHQLMGSQGLLDAEKVENILLILNSAARYPLLPEFSTDNQKPSTLQNAVLKSIKTFELGQQLDVELLMLDQISTIMALPFDTRTRIQMKLGPKLSSSSRKRIPSFEAMSYEACSCFSQRLSGINDLNQSFVSSKRLLKILKSLSIPIINKSLIKGDMNNSAVLWTLSSRCFFSLAEKILDVFHYEEVDHIPEEVRDAFFDMFVEVAVVPLKRLDSETDLLTEIDDIEEFQHFREKLLQQLNLESLKAKHLKKFVSSVWSASFFHEVDDIEDEIIKSSESLEEVAQKLAVFPFDEVLESTRGQKLLSKYKTATLCLQDLTNYMMFEGPGFDKLRAVCLPFLVARYAFSLRRYVSDAILLNRAPIAKDRSMEVALVLSGLRKVLDYISKAKGKVACDKAIRDLQVLYPLILRAIPVSQKVSGLETIVQEVSLDFTKLLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBS2</accession>
    <name>C5DBS2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0A04928p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0A04928g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551671.1">
        <property type="nucleotide sequence ID" value="XM_002551625.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBS2"/>
    <dbReference type="FunCoup" id="C5DBS2">
        <property type="interactions" value="87"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBS2"/>
    <dbReference type="GeneID" id="8290475"/>
    <dbReference type="KEGG" id="lth:KLTH0A04928g"/>
    <dbReference type="eggNOG" id="ENOG502RK42">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051217_0_0_1"/>
    <dbReference type="InParanoid" id="C5DBS2"/>
    <dbReference type="OMA" id="WNTEINE"/>
    <dbReference type="OrthoDB" id="4033625at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.570">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="399"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="403"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="418" mass="47925" checksum="F38F21BC000D347B" modified="2009-07-28" version="1">MDLLNGFYGPVLVERAAFAAQDEFLSPEIKSVWCSAAFEYLGLLQFNGSPHIGTISKFVEGNQGNLIPNASTAKVQVNYNVCRKVVAHEFSDAIALQEADSNVPFLEKLYGIVRLLILGEKWKSLDEIDHRLDAYLKSKEGCLSEAEKHLTLAVRLLICGALYLQGRYFDCIKYFVTAIQKDGAIFDMLLLSDSEAFFTTEEIITMITLSVIVSVPLDNYEEFAQLEELKPLFVIAPTLSSCLSLLIDTSYNSFLKLWNESFDQKCSSSYFLCGRWKEVQATMRYKIYFFYLRISNRVEIRYLSETLGIDFETVRTEINSLIDDLTLNFSINDQLIYYRNRYEMSDIVKNLSSSKTILDERIEKLKNQNDLVRYQVQQSIIENNARLREQNRDLDACNDESNENLQNFVSNSEPDLGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBZ3</accession>
    <name>C5DBZ3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 18">Postreplication repair E3 ubiquitin-protein ligase RAD18</fullName>
            <ecNumber evidence="5 18">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">RING-type E3 ubiquitin transferase RAD18</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="ordered locus">KLTH0A06556g</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">E3 RING-finger protein, member of the UBC2/RAD6 epistasis group. Associates to the E2 ubiquitin conjugating enzyme UBC2/RAD6 to form the UBC2-RAD18 ubiquitin ligase complex involved in postreplicative repair (PRR) of damaged DNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 25">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 18">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="18">Interacts with E2 UBC2, forming a complex with ubiquitin ligase activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 18">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 18">Belongs to the RAD18 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5 18"/>
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    <keyword evidence="7 18" id="KW-0808">Transferase</keyword>
    <keyword evidence="11 18" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="12 18" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 16 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="20">
        <location>
            <begin position="37"/>
            <end position="74"/>
        </location>
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            <begin position="191"/>
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        <source>
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    <sequence length="460" mass="51518" checksum="1F2E56601585468F" modified="2009-07-28" version="1">MSRLPKLSEQIQDVSDPRDFLRTKLPELVDLDTLLRCHICKGFIKTPVLTPCGHTFCSLCIREYLNRELKCPLCLAELRESMLRSEFLVNEIIASYVGLRSKLLEIARSGPDGTVPHSDTSLIEIAPGDEGAHELSGDDDLQIIETRRNRPAKRNAEIVLMPSKKSRRDSEKPGISSMISKSKSPALPEDSAECPICQKHFPLEYLQRTHLDECLTMGSLGAAAKPTSPPKIGKASNETTVSVQPAAERISEPSVSANTTHSNRYLESGQKQGVARLAKLNFSSMSMQQLKSKLTSLKLSTIGSRQQLINRYNHYEMLWNSNFMDSIEPLEEKELRRRLASWEATHNSSEGTLRKGNISKLLNKNSTTRIKDFKTDRFDRKGWILAHQQEFNELLEEARHNLAKANKMKYSASNQDEVEDAEQAESHPNSISCEKGEIPSNQLVVKTEEISTSSQGTTGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCM4</accession>
    <name>C5DCM4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0B04290p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0B04290g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002551927.1"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005965_5_0_1"/>
    <dbReference type="InParanoid" id="C5DCM4"/>
    <dbReference type="OMA" id="DYGQQNI"/>
    <dbReference type="OrthoDB" id="10262720at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034663">
        <property type="term" value="C:endoplasmic reticulum chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10230">
        <property type="entry name" value="ASKHA_NBD_HSP70_HYOU1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Molecular chaperone DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="2"/>
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        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639">
        <property type="entry name" value="HSC70CB, ISOFORM G-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639:SF3">
        <property type="entry name" value="HYPOXIA UP-REGULATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002948664" evidence="7">
        <location>
            <begin position="20"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="836"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="845"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="865"/>
            <end position="884"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR21535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="884" mass="98736" checksum="526CAE7F85B8C2D2" modified="2009-07-28" version="1">MKLSTFIGGLSVFGSIASGALLGFDAGQEFSKAMLVSPHAPLELVLTPDSKRKDVSGLAIKPWKNDIERVYGSGVDSAHVRAPEGALLHIKPLLGKQEEEDLTFYHKSHPGITFVPTERGSIALKCFGHEYPVEQVLAMNLDETIGRANKLLAEKNGLDVVDSLAITVPEFFNQDQRLALKSAAELPHGVKAQLVNDGMSVAIDFALKQRDFPPGEREYYVFYDMGSGSTRASLFAIEQPLNKSEPLMIEFGGYGFDTTLGGAKFTQNVAELLKNKFLEQNPAIRTEQLDRSARSLVRIHQAAEKAKLVLSANVDASVSIESLYDDIDFKAVITRQEFEDFSEDLTGRIVQPIEDALKNQFFENTVDTSNVKSLVLTGGSTRTPIVQKQLTHYLGSDLIARNVNADESSVNGVTIRGIQLFKSFQTKAIDILDRSIFSYGIIVNGSASPVEIFPRGSQYPNKTSFLIPPTNYTREFSIDLKENGNTFKKHVIETGPIQERFSEEICPQGVAFNATFTLSQERIFDLDSVEAICLHNSKNSTGLFKKLFSGGTPRQEQDTIGKGDLDDIVPHKVKMLKTSESFTHVGPMSITEKLRVRQHIDDLNQKDKMRLQTQELLNALESILYSTRAFLEQEDVIAGGPRQDIDSLSEYVTVNLEWLDYESDEASVAELMEKIDHISSLKDKIELYLMSSGEPLSFEQFSELHTNGSRLVQELRGIEVTANATLEALKDAFSEIGLDVAKEFKKLKAPRHLSSPNYKLKDKIEAFEKNLADISSLLSAQDFDSFSREELFELKLIFDSSFDDLRKRLELEQAIQNFKMNELASLHSRRLRVLKKREERRKSQESSTSLNASNSTLTSTTTESTKSEEEASKAEEFTIDHDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCW6</accession>
    <name>C5DCW6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0B06358p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0B06358g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552065.1">
        <property type="nucleotide sequence ID" value="XM_002552019.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCW6"/>
    <dbReference type="FunCoup" id="C5DCW6">
        <property type="interactions" value="97"/>
    </dbReference>
    <dbReference type="GeneID" id="8290904"/>
    <dbReference type="KEGG" id="lth:KLTH0B06358g"/>
    <dbReference type="eggNOG" id="KOG3989">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087038_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCW6"/>
    <dbReference type="OMA" id="CWVEYCA"/>
    <dbReference type="OrthoDB" id="1898221at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006838">
        <property type="entry name" value="ADTRP_AIG1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10989">
        <property type="entry name" value="ANDROGEN-INDUCED PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10989:SF16">
        <property type="entry name" value="AT02829P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04750">
        <property type="entry name" value="Far-17a_AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="49"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="120"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="232"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR21627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="263" mass="28354" checksum="E0BFC26D0589C68F" modified="2009-07-28" version="1">MRLPRQPQPQYKKTAPAGGAGLDRPAYAVVHSRDYDFDSTTMRANGRTMTCAALNALCLCTSTWGLWRATEVRLPASLAGAGHRQFLTILAVVATIITNVANLGALAAGGARARRAARQVALPVALVVETVVAVVYWPLRLFALPLIMHDVKDGSRMPLAVSVDCAVHLAPVVLLGADYALVEQRPFEMSLGRAWVVVTALGLGYRRYLETMVDRAGGAAYPYPFLDVAEPYQSGIFVVVTTAAWAVFWGYKKVHAGRASGRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DD86</accession>
    <name>C5DD86_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0B09174p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0B09174g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552185.1">
        <property type="nucleotide sequence ID" value="XM_002552139.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DD86"/>
    <dbReference type="FunCoup" id="C5DD86">
        <property type="interactions" value="95"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DD86"/>
    <dbReference type="GeneID" id="8291028"/>
    <dbReference type="KEGG" id="lth:KLTH0B09174g"/>
    <dbReference type="eggNOG" id="ENOG502S656">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065173_0_0_1"/>
    <dbReference type="InParanoid" id="C5DD86"/>
    <dbReference type="OMA" id="MKNNIDL"/>
    <dbReference type="OrthoDB" id="4977at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005869">
        <property type="term" value="C:dynactin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028133">
        <property type="entry name" value="Dynamitin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15346">
        <property type="entry name" value="DYNACTIN SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04912">
        <property type="entry name" value="Dynamitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="95"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="321"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR21747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="364" mass="41561" checksum="BF327D745ABF6A1B" modified="2009-07-28" version="1">MALDIIEWDEEAAVGGIPNDSFKDGKEVFETSDIEETESETSVPALMNDDINAIKDDYDRSRELFENQVLENIGSDFSGRTDRLQSAHMVSGSCLETRAQKLARIRRELQEIELSEESGEANSTELVSLRQLLDQLDSKAKSESQNIKQLLCESDKVREQVELPKINLELGLARRLAELENKVSKLESVLGLSGYSSQKTITTTMDELYRQIKLLRNNDKLLNEFRSKLESISANYEKTLIARKASRNSSLQKQISSDLISSETKVSELYESFSILRRYKDVVPHITTRMKTLNSLHMEIGDCVSTVKTVDSSVKWMTDQNEKWQKMLIDMDEKLESVEKKAEANKQEVTKWLTNVESRLQEMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDF6</accession>
    <name>C5DDF6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0C00616p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0C00616g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552255.1">
        <property type="nucleotide sequence ID" value="XM_002552209.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDF6"/>
    <dbReference type="FunCoup" id="C5DDF6">
        <property type="interactions" value="648"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDF6"/>
    <dbReference type="GeneID" id="8291113"/>
    <dbReference type="KEGG" id="lth:KLTH0C00616g"/>
    <dbReference type="eggNOG" id="KOG0294">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031466_2_0_1"/>
    <dbReference type="InParanoid" id="C5DDF6"/>
    <dbReference type="OMA" id="IIIWRTK"/>
    <dbReference type="OrthoDB" id="308449at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051959">
        <property type="entry name" value="PAK1-Kinase_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44675:SF1">
        <property type="entry name" value="P21-ACTIVATED PROTEIN KINASE-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44675">
        <property type="entry name" value="PAK1 INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="37"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="138"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="290"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="362"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="363"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="387"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR21817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="407" mass="46322" checksum="1492740C23B34551" modified="2009-07-28" version="1">MSQNQFRIVVGSYEHNLLCLSLNLSLPSPLFTPIFHFQAHSLSVKCLDISKRYLVSGSNDEHIRIYDLQKRKELGTLLSHQGSITSLKFSRGAAIPNQDGDDSEKPVTHGNSKWLLSASEDHNLVVWRVKDWENFGTLKGHSARINDFDIHPSNRIAISVSEDHSIRLWNLMTVKKAGILKLKKYNQNGQFVRWCGDNGEFFAVALLNKVLIYKTSTAKVHREIDVGRSTIMHLEVEKINDKQYIVVALGNGSVSMYQGEQLFKDDADEAEEAEKTGDAEETPITPEYTLQGHATRVKDFKFYRNEFGHYLVTISSDGKIVVWDMSNREQVAVYDCGERLNCLAICDESVERYEMMKKRVAEEADLGEQSEVEEDTEDLKSIMLGTNKKKSKKSRSKKNKKVKVQLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDL7</accession>
    <name>C5DDL7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0C02024p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0C02024g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the exportin family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552316.1">
        <property type="nucleotide sequence ID" value="XM_002552270.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDL7"/>
    <dbReference type="FunCoup" id="C5DDL7">
        <property type="interactions" value="1556"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDL7"/>
    <dbReference type="GeneID" id="8291175"/>
    <dbReference type="KEGG" id="lth:KLTH0C02024g"/>
    <dbReference type="eggNOG" id="KOG2020">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011906_0_0_1"/>
    <dbReference type="InParanoid" id="C5DDL7"/>
    <dbReference type="OMA" id="WAFKHNN"/>
    <dbReference type="OrthoDB" id="27218at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005049">
        <property type="term" value="F:nuclear export signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051170">
        <property type="term" value="P:import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000056">
        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000490">
        <property type="entry name" value="CRM1p Major karyopherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041123">
        <property type="entry name" value="CRM1_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041235">
        <property type="entry name" value="Exp1_repeat_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013598">
        <property type="entry name" value="Exportin-1/Importin-b-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045065">
        <property type="entry name" value="XPO1/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014877">
        <property type="entry name" value="XPO1_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040485">
        <property type="entry name" value="XPO1_repeat_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11223">
        <property type="entry name" value="EXPORTIN 1/5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11223:SF2">
        <property type="entry name" value="EXPORTIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08767">
        <property type="entry name" value="CRM1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18777">
        <property type="entry name" value="CRM1_repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18784">
        <property type="entry name" value="CRM1_repeat_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18787">
        <property type="entry name" value="CRM1_repeat_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08389">
        <property type="entry name" value="Xpo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01102">
        <property type="entry name" value="CRM1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="6">
        <location>
            <begin position="34"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR21878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1084" mass="123748" checksum="7D44604BD771E767" modified="2009-07-28" version="1">MESILDFTQDLDINALDQVVDTFYKGSGVQQKQAQEVLTKFQDHPDSWQRADQILQFSQNPQTKFIGLSILDKLINTKWKLLPPEHRVGIRNFIVGMIISLCQDDAVFQSQKNLINKSDLTLVQILKQEWPQNWPDFIPELVQSSQSSVNVCENNMVILKLLSEEVFDFSAEQMTQAKALHLKTSMSKEFEQIFKLCFQVMDAGSTTSLVLAALESLLRYLHWIPYHYIYNSNLLDLLSTKFLMSSDTRAVTIKCLTEISNLEIPQNDPKIAEQTVLFFQNTLQQVGNNVVPVTADLKATYATANGRDQSFLQDFAMLLTTYLARHRALLESDERLRELLLTAHQYLIQLSKIDERELFKTTLDYWHHLVAHLFQEVQTLPVAELNPLLKLSVGSQAINSSGGAPNPEFLKRFPLKKHIYDGICSQLRWVVIESMVRPEEVLIVENDEGEIVREFVKESDTIQLYKSEREVLVYLTHLDVVDTEDIMINKLARQIDGSEWSWHNINTLCWAIGSISGTMSEETEKRFVVTVIKDLLALTEKKRGKDNKAVVASNIMYVVGQYPRFLKAHWKFLKTVVIKLFEFMHETHEGVQDMACDTFIKIVKKCKHHFGVQQPNEPEPFIHTIIREIQSTTADLQPQQVHTFYKACGIIISDERNATERERLLADLMQLPNMAWDAIVEQSAANPDLLLDPETVKIIANILKTNVAVCSTMGAEFYPQLGHIYYNMLQLYRAVSSMISVQVAKDGLIATKTPKVRGLRTIKKEVLKLVEFYISHAKNLEEVVKVLVEPLLNAVLEDYMNNVPDARDAEVLNCMTTVVHKVGHMIPSGVILILQSVFECTLDMINKDFTEYPEHRVEFYRLLKEINSRSFNALLELPSAAFKLFVDAICWAFKHNNRDVEVNGLQLALDLIKNIEAMGSTPFSNAFYENFYFTFISETFYVLTDSDHKSGFSKQSLLLMRLISLVQENKIAVPLYQPGDVPEGTTNQLYLANYLAGMLNGAFPHLSQEQVTSFISALIKQYQDPAKFSATLRDFLVQIKEFGGDPTDYLFAEDKERALEEKTRMEKERAAAVGGLLKPSELED</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDN4</accession>
    <name>C5DDN4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0C02420p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0C02420g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552333.1">
        <property type="nucleotide sequence ID" value="XM_002552287.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDN4"/>
    <dbReference type="FunCoup" id="C5DDN4">
        <property type="interactions" value="178"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDN4"/>
    <dbReference type="GeneID" id="8291193"/>
    <dbReference type="KEGG" id="lth:KLTH0C02420g"/>
    <dbReference type="eggNOG" id="ENOG502S0PT">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_418607_0_0_1"/>
    <dbReference type="InParanoid" id="C5DDN4"/>
    <dbReference type="OMA" id="HANNHHF"/>
    <dbReference type="OrthoDB" id="4065296at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="206"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="159"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="214"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="253"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="322"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="360"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="464"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="495"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="512"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="534"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="557"/>
            <end position="568"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="568" mass="61665" checksum="A859D9BFDA1C61BA" modified="2009-07-28" version="1">MEHVSEMSRTEIPLDATTTVASPLAPDSENSPQESDASVLESSGVSKEGSAPKLQQITNIEEFKPQSETKTYVYSMKELMKISKVVSQSAQSGATANLPKKSFWRLTSRHPDIRQVESAGGNGNGHKNDGNSRGQDSGNEKKGHRNRNSRNGRRGSKFSKGEKPFVEEKDIKVNNDELLALEEEIKPTGNSITDFENWRAKMKELERKKKGIASKNSTGSQERPPLTNNSSSISDFFNLKRESSSNFEELEPQGTSSENMKGSHSRFSSFFNSTPPPGLSGAQSTVAKPPTPKEEDARPAAGSRILSFFDKMDSQKHAASSVEPNIQMAEQISQTKAAPVHEQTNSHFFQGLLNRGKASPGNTERNNSNREASGPSALISPNTIPTSKVAEQPKEQAARSPAGPPGLSKLPPNKHVMSSQPQPGFLTGMPPNVYSSNSQPMGAPPSGFQHFQMPLPGVAVPQQFFANPRQTGDFTGSQQKDDNSSRPSNMQIPYMPNPRGIPPPGIPPMHMMPPGISQDMPLPMNGMMPPPSFYLSQGPSFGQFNNPPMPQNGMVGQPQSQNLPPRKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDP1</accession>
    <name>C5DDP1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0C02574p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0C02574g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552340.1">
        <property type="nucleotide sequence ID" value="XM_002552294.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDP1"/>
    <dbReference type="STRING" id="559295.C5DDP1"/>
    <dbReference type="GeneID" id="8291200"/>
    <dbReference type="KEGG" id="lth:KLTH0C02574g"/>
    <dbReference type="eggNOG" id="ENOG502QREF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008074_0_0_1"/>
    <dbReference type="InParanoid" id="C5DDP1"/>
    <dbReference type="OMA" id="WYDWADL"/>
    <dbReference type="OrthoDB" id="444255at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009100">
        <property type="term" value="P:glycoprotein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009644">
        <property type="entry name" value="FKTN/MNN4/W02B3.4-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007074">
        <property type="entry name" value="LicD/FKTN/FKRP_NTP_transf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15407">
        <property type="entry name" value="FUKUTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15407:SF28">
        <property type="entry name" value="RIBITOL-5-PHOSPHATE TRANSFERASE FKTN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04991">
        <property type="entry name" value="LicD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="LicD/FKTN/FKRP nucleotidyltransferase" evidence="6">
        <location>
            <begin position="570"/>
            <end position="808"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR21902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1032" mass="116504" checksum="31CD222175DA0599" modified="2009-07-28" version="1">MRTQLGRNSRRLITLLLVTVLAEVVYIVVSVVLVEDGREAGIAGLARGVSGGVGAGASRLERWLLGGSAAVFRADPNRMLPGLTSGSRVRLPDRDVEYVVRGLRRSSDGDGDSGKASAAPSASDLVNFYRAWKFDTAVRYVLPCSAAQTLFGRLQRPVENLETLFIGAGEAAEGPAEPAVKAGGEAGDNHVTGDGAAAEGRNLQFPLRSYNYDPRLATAVYYDELLNHLEHQEVHDAESLFANLSVPFNWYDWTNSQLLNGFIDLPPAEKPACDMIVRKYYPADKVLAFERRFGYKLFEEDRARGLGAAKFDSVSAFSHSTPRLPRPEELCDSDQANALLPGFRMLKPLNFSRPELYGLQARATLMTTAEPPNSITFLNRNGSTLQVEVDAGAPLDSAGVPRGLLFNGLLRNYLRRNVPSYPEFPARDIEFDNIQKFKALKELVTKSLTRPSTASAEPAEPTEPVQPTEMPYLIEMSNDEFEFDAKAKIRELEALETRTRHQQSYLESLKFSINTHPMHLAKYFNEASSVSDYVHLGHHFDARFFRGGVEHTDMRARLDALVRAWLNFVHSNGLNSWLSHGTMYGWLYDGLAFPWDGDHDMQMPIVHLNKLAEKFNQTLIVEDPEVGNGRFFLDVTSSITSRIHGNNNNNIDARFIDVDSGLYVDITGLSVSSDGANKRFDPLIKNFTTQNNISSLTHFKDPNLVEGITGLSYDEMVKAETKQYGNFTKQREHVLKSYFNTVNSRQNGKDKESPDYRYNINKHIQVYNCRNNHYNHLSELSPLRLTFFHGAKAYVPNRVIQILKNEYHAPSNYSYIEFQGRSFVPEFRLWIGKGGLERMAQGAGVPTRVDTDHLKNLDFPETASLFQNAAVLADSEIEEFFSLATNTFHMSIFRQKELELTYDESRSPDQKVEALRGFVSNRAFTGGYKDPFQQRLETVVWYNLLDRSTISYKQLDRELKKAHLENSDKLLELNDLMIRREFDWAREAGYQYPPSDLDFNNLGSRFFAIGENSHNQIFDADPPELVQEWTTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DE31</accession>
    <name>C5DE31_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Mediator of RNA polymerase II transcription subunit 19</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">Mediator complex subunit 19</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MED19</name>
        <name evidence="11" type="ordered locus">KLTH0C05874g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the Mediator complex subunit 19 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DE31"/>
    <dbReference type="FunCoup" id="C5DE31">
        <property type="interactions" value="98"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DE31"/>
    <dbReference type="KEGG" id="lth:KLTH0C05874g"/>
    <dbReference type="eggNOG" id="ENOG502QXG3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117719_0_0_1"/>
    <dbReference type="InParanoid" id="C5DE31"/>
    <dbReference type="OMA" id="SYPTEFQ"/>
    <dbReference type="OrthoDB" id="2160599at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013942">
        <property type="entry name" value="Mediator_Med19_fun"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28270">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28270:SF1">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08633">
        <property type="entry name" value="Rox3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0010">Activator</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="169"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR22042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="203" mass="23045" checksum="2443125300A4868E" modified="2009-07-28" version="1">MTTVDDHISPAYYYYVDPATVYQAQEPNPLEDLISVYGLQDLSRQVARTNPDGSKAVKLRKSYKNQISDLSAKFSVIPTRENGKGGDISHMLFQNNADMMAQVRRTPEMTAEQWRQLMAERDAGLFDGHNMDWEICQTVLSQFERSYPAEFQANGFQAEDLAFDLDGSGNTLKSRKRKNKSSGSSMATPNSDLQDDMKRRRLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DE52</accession>
    <name>C5DE52_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0C06336p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0C06336g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552501.1">
        <property type="nucleotide sequence ID" value="XM_002552455.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DE52"/>
    <dbReference type="STRING" id="559295.C5DE52"/>
    <dbReference type="GeneID" id="8291369"/>
    <dbReference type="KEGG" id="lth:KLTH0C06336g"/>
    <dbReference type="eggNOG" id="ENOG502R28W">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_306755_0_0_1"/>
    <dbReference type="InParanoid" id="C5DE52"/>
    <dbReference type="OMA" id="ANMQAPR"/>
    <dbReference type="OrthoDB" id="5600002at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45093:SF2">
        <property type="entry name" value="LISH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45093">
        <property type="entry name" value="TRANSCRIPTION ACTIVATOR MSS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08513">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0010">Activator</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="217"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="398"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="504"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="581"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="702"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="957"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="217"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="228"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="504"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="520"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="581"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="627"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="640"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="739"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="830"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="842"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="866"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="877"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="905"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="967"/>
            <end position="978"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR22063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1011" mass="111489" checksum="D39EF7E153B18EB0" modified="2009-07-28" version="1">MSLGPGGLHMNEDYAPQAQRPVQGVRAQNGRPTPRAPAGTQQGATPASARTGTPHPTASSREDPKRLLNAYIYDFLSKSSLNETAEAFSREAVIEENGQRPTQDNRFKPQNDAPQGFLYEWWQIFWDVFNARTHRGGSEMAQQYFQLQFDSRRQEHTYRNVAMHAARMQQQVEQRGDYQGEVFDPMMFAMMFSGNAYAAPMYDKSTDNGMRPTAFQRQVPSQQLQNSQLQARSARPPQRQQEPQSQVHAPGQTQAHAQAQAQAQAQAQAQAQAQAQVQAQAQAQAQAQAQAQAQAQAQAQARAQAQARAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQVQAQAQAQAQAQAQAQAQAQAQAQAQAEAQAQAQAQAQAQAQAQAQVHAQQMRQRQAPMAASHVQQHAQPQHRQQQQTMYAQLMQQQQQRQHQQLQQQQQQPQSSQMYAQQMMMNGGNPNALALQGANAPVYMGPVRNVPMKPNFQASYMPQAVQPQMQQKHQLSPAHQHQQQRSPQHQQQQQQQQQQQHQQHQQHQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQHQHQNQQQQHQQPHPQQYPQQHHQNQNQNQQHQQHQQQRHQQRQQHPQQYPQQQQQVASANPSGYANPSSAKKRLPQSDGSPDTDGVVKPDAHLSSTEGLYNYQKQLLMLEKENEKVVTYRSTASTPGLGGPPQQSSGAPNKPSSVAGYAGVSPLPINSINSPASLGTAPKSKEAMRRKSISTQATGPAVNAGRKNQSEPVTPIGPMTPASGSLNRLAPPSALSTVTETPLKASGEQADRMAGKTTSAKRVKRLKKMPSANNLGTGARPSSPSLDNVLEAKNSKRISRAKPKKSSGVSGIKVSRIARSAGTTPLTTEDVLPGSTSSNDTGSNVFPASVATDPTPQTDFDPLGQNDPTLGTTNYDFEDDDIFNIDSGLLDKSAPSKAQSTTQSAQRPQDDLPIDEPFNLDFLDPASTMNYNDLNQLNWQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DE65</accession>
    <name>C5DE65_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Diphthamide biosynthesis protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0C06644g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>2 [3Fe-4S](0)-[protein] + 2 Fe(2+)-[Dph3] + NADH = 2 [4Fe-4S](1+)-[protein] + 2 [Dph3] + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:71239"/>
            <dbReference type="Rhea" id="RHEA-COMP:17997"/>
            <dbReference type="Rhea" id="RHEA-COMP:17998"/>
            <dbReference type="Rhea" id="RHEA-COMP:18001"/>
            <dbReference type="Rhea" id="RHEA-COMP:18002"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:33723"/>
            <dbReference type="ChEBI" id="CHEBI:47402"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:83228"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>[3Fe-4S](1+)-[protein] + Fe(2+)-[Dph3] = [3Fe-4S](0)-[protein] + Fe(3+)-[Dph3]</text>
            <dbReference type="Rhea" id="RHEA:71235"/>
            <dbReference type="Rhea" id="RHEA-COMP:17996"/>
            <dbReference type="Rhea" id="RHEA-COMP:17997"/>
            <dbReference type="Rhea" id="RHEA-COMP:18002"/>
            <dbReference type="Rhea" id="RHEA-COMP:18003"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:33751"/>
            <dbReference type="ChEBI" id="CHEBI:47402"/>
            <dbReference type="ChEBI" id="CHEBI:83228"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the DPH3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552514.1">
        <property type="nucleotide sequence ID" value="XM_002552468.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DE65"/>
    <dbReference type="FunCoup" id="C5DE65">
        <property type="interactions" value="365"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DE65"/>
    <dbReference type="GeneID" id="8291382"/>
    <dbReference type="KEGG" id="lth:KLTH0C06644g"/>
    <dbReference type="eggNOG" id="KOG2923">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_155991_4_2_1"/>
    <dbReference type="InParanoid" id="C5DE65"/>
    <dbReference type="OrthoDB" id="66964at2759"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.660.10:FF:000001">
        <property type="entry name" value="Diphthamide biosynthesis 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.660.10">
        <property type="entry name" value="DPH Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044248">
        <property type="entry name" value="DPH3/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007872">
        <property type="entry name" value="DPH_MB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036671">
        <property type="entry name" value="DPH_MB_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21454:SF31">
        <property type="entry name" value="DIPHTHAMIDE BIOSYNTHESIS PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21454">
        <property type="entry name" value="DPH3 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05207">
        <property type="entry name" value="Zn_ribbon_CSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144217">
        <property type="entry name" value="CSL zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51074">
        <property type="entry name" value="DPH_MB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DPH-type MB" evidence="9">
        <location>
            <begin position="38"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00036267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00041070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR22076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="117" mass="13360" checksum="292875C96F9F95F4" modified="2009-07-28" version="1">MNKKAHRDEYDKTRIPAPAIIRAPRPLPALLQEDDMSTYDQVEIEDMTFDPDTQTFTYPCPCGDKFQIYIDDMYDGEEIAVCPSCSLMIQVIFEREDLEEYYQEAGTAPPEPVSVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5DE94</accession>
    <name>C5DE94_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">3-hydroxyisobutyryl-CoA hydrolase</fullName>
            <ecNumber evidence="2">3.1.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0C07304g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>3-hydroxy-2-methylpropanoyl-CoA + H2O = 3-hydroxy-2-methylpropanoate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20888"/>
            <dbReference type="ChEBI" id="CHEBI:11805"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57340"/>
            <dbReference type="EC" id="3.1.2.4"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.1.2.4" evidence="2"/>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552543.1">
        <property type="nucleotide sequence ID" value="XM_002552497.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DE94"/>
    <dbReference type="FunCoup" id="C5DE94">
        <property type="interactions" value="750"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DE94"/>
    <dbReference type="GeneID" id="8291412"/>
    <dbReference type="KEGG" id="lth:KLTH0C07304g"/>
    <dbReference type="eggNOG" id="KOG1684">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_22_0_1"/>
    <dbReference type="InParanoid" id="C5DE94"/>
    <dbReference type="OMA" id="AYRNNEH"/>
    <dbReference type="OrthoDB" id="1737613at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003860">
        <property type="term" value="F:3-hydroxyisobutyryl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006574">
        <property type="term" value="P:L-valine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045004">
        <property type="entry name" value="ECH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032259">
        <property type="entry name" value="HIBYL-CoA-H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43176:SF3">
        <property type="entry name" value="3-HYDROXYISOBUTYRYL-COA HYDROLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43176">
        <property type="entry name" value="3-HYDROXYISOBUTYRYL-COA HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16113">
        <property type="entry name" value="ECH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl-CoA hydratase/isomerase" evidence="4">
        <location>
            <begin position="28"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR22105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="476" mass="52541" checksum="A58AC3C70C72A5FD" modified="2009-07-28" version="1">MLRSSLRCHQIKRFMSEQVKFGSKGVSRVVTLSRPEKLNALSDQMCRKMIPTLQEYAKSETNNIVVIGSANAPRSLCSGGDVATVALQNIAGKRDLSLEFFRNEYSLNLLLATYNKPVVVLMDGITMGGGVGLATHVPFRIATENTRWCMPEMDIGFFPDVGTTFSLPQLTTVGGTKGQLALYLCLTGDLLSGIDVYMAGLASHYVPSHNLVDLQARLGELPVTNSEEMWQITGNAIEEFSVGIPEDYNFKYSNAQLDVIESCFDIGHGWKGINTALEKVVNSSSASPEEKEFARLTLQKLGTKSPVSVQVAIEQFRRNSQTDVESALKQDLVTASNMCEDPASEFSQATKHKLVDKSKSAFPWTKTSLTLEELSRLVSPRASASVSLQKFDKAVTWKKYPYHSQFMLPTEQNFKDYITGNDNSGRSLAANQAEVVKYFSQYNSSSKGKTGIEYLCNLVCSRKCTVGDAGELRWNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEB2</accession>
    <name>C5DEB2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0C07766p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0C07766g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552561.1">
        <property type="nucleotide sequence ID" value="XM_002552515.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEB2"/>
    <dbReference type="GeneID" id="8291433"/>
    <dbReference type="KEGG" id="lth:KLTH0C07766g"/>
    <dbReference type="HOGENOM" id="CLU_1532851_0_0_1"/>
    <dbReference type="InParanoid" id="C5DEB2"/>
    <dbReference type="OrthoDB" id="10354008at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="175" mass="18623" checksum="00423C39651A96B1" modified="2009-07-28" version="1">MVIYVSVEDITQEDVYDFLETELEQLNEDTERFVTGGLVHEDSYTTAYDAMLIARTRALGKISDEAADPECGADTPAQEITDVDQSSDPAVPSPSSMLAKAHKRALKHTERDEMDRAIAAGALAYLHNLAKQGFLLREGGPASFLLGYVWVAPGLPGGPGAAPEKSGPAESGGAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEF8</accession>
    <name>C5DEF8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">3'(2'),5'-bisphosphate nucleotidase</fullName>
            <ecNumber evidence="3 11">3.1.3.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0C08822g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Converts adenosine 3'-phosphate 5'-phosphosulfate (PAPS) to adenosine 5'-phosphosulfate (APS) and 3'(2')-phosphoadenosine 5'-phosphate (PAP) to AMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>3'-phosphoadenylyl sulfate + H2O = adenosine 5'-phosphosulfate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:77639"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58243"/>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
            <dbReference type="EC" id="3.1.3.7"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:77640"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>adenosine 2',5'-bisphosphate + H2O = AMP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:77643"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:194156"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.1.3.7"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:77644"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>adenosine 3',5'-bisphosphate + H2O = AMP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10040"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.1.3.7"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:10041"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10 11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the inositol monophosphatase superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.7" evidence="3 11"/>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552607.1">
        <property type="nucleotide sequence ID" value="XM_002552561.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEF8"/>
    <dbReference type="FunCoup" id="C5DEF8">
        <property type="interactions" value="88"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEF8"/>
    <dbReference type="GeneID" id="8291481"/>
    <dbReference type="KEGG" id="lth:KLTH0C08822g"/>
    <dbReference type="eggNOG" id="KOG1528">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033446_2_1_1"/>
    <dbReference type="InParanoid" id="C5DEF8"/>
    <dbReference type="OMA" id="MSYQQER"/>
    <dbReference type="OrthoDB" id="411145at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008441">
        <property type="term" value="F:3'(2'),5'-bisphosphate nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043647">
        <property type="term" value="P:inositol phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000103">
        <property type="term" value="P:sulfate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01517">
        <property type="entry name" value="PAP_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.10">
        <property type="entry name" value="Fructose-1,6-Bisphosphatase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006239">
        <property type="entry name" value="DPNP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020583">
        <property type="entry name" value="Inositol_monoP_metal-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051090">
        <property type="entry name" value="Inositol_monoP_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020550">
        <property type="entry name" value="Inositol_monophosphatase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01330">
        <property type="entry name" value="bisphos_HAL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43200:SF6">
        <property type="entry name" value="3'(2'),5'-BISPHOSPHATE NUCLEOTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43200">
        <property type="entry name" value="PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56655">
        <property type="entry name" value="Carbohydrate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00629">
        <property type="entry name" value="IMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00630">
        <property type="entry name" value="IMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 10 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="10">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR600760-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR22169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="360" mass="39037" checksum="4EB778C1456762B6" modified="2009-07-28" version="1">MPFEKELFVATQAVRKAALLTKRIQAQVIADRKSTITKTDSSPVTVGDYSAQAIIINAIKANFPEDEIVGEEESKGLNDEFVGQILREIEATEAPFKELFGEYDSVRAGMQFRSREYPLTSLADVRRVIDLGSSEGGRKTRFWCLDPIDGTKGFLRGEQYAVCLALVVGGVVQLGCIACPNLELANYGGKDSDPEAAHRLGYVFRAVRGHGAFFAPTAVDFSWHPLPVRSLSSTRDMVSLEGVEKGHSAHSAQDAVKEELGISRALHLDSQVKYCLLALGLGDVYLRLPISLSYREKIWDHAAGNALVAETGGQHTDSIEGVPLDFGNGRTLATKGVIASSGTKLVHQMIVDSSSKAINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEJ9</accession>
    <name>C5DEJ9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0C09878p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0C09878g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VPS52 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552648.1">
        <property type="nucleotide sequence ID" value="XM_002552602.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEJ9"/>
    <dbReference type="FunCoup" id="C5DEJ9">
        <property type="interactions" value="924"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEJ9"/>
    <dbReference type="GeneID" id="8291528"/>
    <dbReference type="KEGG" id="lth:KLTH0C09878g"/>
    <dbReference type="eggNOG" id="KOG1961">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010797_1_0_1"/>
    <dbReference type="InParanoid" id="C5DEJ9"/>
    <dbReference type="OMA" id="VFRLECE"/>
    <dbReference type="OrthoDB" id="19482at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000938">
        <property type="term" value="C:GARP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007258">
        <property type="entry name" value="Vps52"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048361">
        <property type="entry name" value="Vps52_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048319">
        <property type="entry name" value="Vps52_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14190">
        <property type="entry name" value="SUPPRESSOR OF ACTIN MUTATIONS 2/VACUOLAR PROTEIN SORTING 52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14190:SF7">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 52 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20655">
        <property type="entry name" value="Vps52_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04129">
        <property type="entry name" value="Vps52_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Vps52 coiled-coil" evidence="6">
        <location>
            <begin position="48"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="Vps52 C-terminal" evidence="7">
        <location>
            <begin position="235"/>
            <end position="561"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR22210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="590" mass="68016" checksum="3617CBB70F076C5A" modified="2009-07-28" version="1">MDALQTILGTQVQAIPPSDGTDLYEGFLEHAKEHRFQVDNSSLTELHNLEERSKAIEQAIEEKIPPLQSYFSGLNDKFKSLTRDLSLIRGKSLELNKLSQENSKDLAGISPLVNDLIISPEVVSQITRGKINASWVENAAYIKDKQEIYAKYKEENWEVPSDFAKLSELLDVLQSLVLERSKKFIVSRIKILRDHRTVPSQRIQSQLLEVKEIFQYLVKHNYSLALELRQAYAYTMRWYYKQYFSRYMRSLTILQYVSIDSNYALGRGLSNSATASGRFASYFSGGYKSSMIGSSNPSDAAINDYFQIEKRLSVLTQEDNTVMVSQIAENNNAPNFLEVGFKNLNLAILDNCSVEFTFLNDFFQVSNNVEDLRGILEQIFQPTFEDAMAYTKRLVTPTFDMFGVLISIWISHHLQFESQRRKVPVVDEYLDGQLILLWPKFQQLVDFQCESLRNFPISVHAKTHSARKEEPDPSTTIQDLTVQFANFLNSILMLSMTQKEHIDERSEPLYNSVIRIRNDFETVMTKCSKKTNHPERFLATNYTYLLNALQQNYVSRERQQHDDTAFKPSLIFQETKTHLEALVEAFSSIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEN2</accession>
    <name>C5DEN2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0C10626p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0C10626g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the OPA3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552681.1">
        <property type="nucleotide sequence ID" value="XM_002552635.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEN2"/>
    <dbReference type="STRING" id="559295.C5DEN2"/>
    <dbReference type="GeneID" id="8291562"/>
    <dbReference type="KEGG" id="lth:KLTH0C10626g"/>
    <dbReference type="eggNOG" id="KOG3335">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074707_3_2_1"/>
    <dbReference type="InParanoid" id="C5DEN2"/>
    <dbReference type="OMA" id="WAEFEGT"/>
    <dbReference type="OrthoDB" id="2129069at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019216">
        <property type="term" value="P:regulation of lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010754">
        <property type="entry name" value="OPA3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12499:SF0">
        <property type="entry name" value="OPTIC ATROPHY 3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12499">
        <property type="entry name" value="OPTIC ATROPHY 3 PROTEIN OPA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07047">
        <property type="entry name" value="OPA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="109"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR22243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="139" mass="15678" checksum="FD7C94B7EA4B3EF8" modified="2009-07-28" version="1">MSGLALKLGTLLIRQVTRPVANVLKAQAKQHDTFKKVCVGLAQKMHRVDARLRTRMTPGNREIKIRPLNDARAVENGATWLSEAFVFGVTGSVVVWETLRQRTKELNRREQVANDISFLQSEIESLREKLDAKERAAGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEP6</accession>
    <name>C5DEP6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0C10956p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0C10956g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552695.1">
        <property type="nucleotide sequence ID" value="XM_002552649.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DEP6">
        <property type="interactions" value="1225"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEP6"/>
    <dbReference type="GeneID" id="8291577"/>
    <dbReference type="KEGG" id="lth:KLTH0C10956g"/>
    <dbReference type="eggNOG" id="KOG4204">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001360_2_1_1"/>
    <dbReference type="InParanoid" id="C5DEP6"/>
    <dbReference type="OMA" id="MCEEVIK"/>
    <dbReference type="OrthoDB" id="10265969at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033698">
        <property type="term" value="C:Rpd3L complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010628">
        <property type="term" value="P:positive regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1160.11:FF:000001">
        <property type="entry name" value="Paired amphipathic helix protein Sin3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1160.11:FF:000002">
        <property type="entry name" value="Paired amphipathic helix protein SIN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1160.11:FF:000003">
        <property type="entry name" value="Paired amphipathic helix SIN3-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1160.11">
        <property type="entry name" value="Paired amphipathic helix"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013194">
        <property type="entry name" value="HDAC_interact_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003822">
        <property type="entry name" value="PAH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036600">
        <property type="entry name" value="PAH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039774">
        <property type="entry name" value="Sin3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031693">
        <property type="entry name" value="Sin3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12346:SF0">
        <property type="entry name" value="SIN3A, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12346">
        <property type="entry name" value="SIN3B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02671">
        <property type="entry name" value="PAH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08295">
        <property type="entry name" value="Sin3_corepress"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16879">
        <property type="entry name" value="Sin3a_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00761">
        <property type="entry name" value="HDAC_interact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47762">
        <property type="entry name" value="PAH2 domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51477">
        <property type="entry name" value="PAH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone deacetylase interacting" evidence="9">
        <location>
            <begin position="665"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="71"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="278"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="424"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="978"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1275"/>
            <end position="1298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1385"/>
            <end position="1433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="87"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="284"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="296"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="449"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1412"/>
            <end position="1421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR22257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1433" mass="163752" checksum="A1F74C9AD2FA2118" modified="2009-07-28" version="1">MPPSWTNQPASQTGTKSSVESPSLSEQQPVSGGMQGQAANSTGESKANAPVDASQQPHQFVLPSLSGLDAGTRAEYGSEPQRLPSLQGAQPQSISSGGSKGFSVASLNNPLPAQQQMLHSQQQQQLLQQGVLPLEHVQDPSYRPLNFKDALSYLEQVKFQFNNRPDVYNHFLDIMKDYKSQAIDTLGVIERISTLFKGYPILIQGLNTFLPQGYKIECTLNPNDPHSIKVTTPFDNARELNLATMATGNGIVEGHPSLMPSDDVSPPKLVGHEPAAVQPKLENAPTNLPVPTTFNQNLPQHEQQNQQHGSQSPAMPQQQRQDQQFMGNRPSGDVEFSHAISYVNKIKTRFADQPEIYKHFLEILQTYQREQKPIHEVYAQVTSLFQNAPDLLDDFKKFLPDATAAHEQQQLLLQQQQQQQQQLQQQQIQQHQSHYQNASQDLDQRQQHTHQPFGNTVPFYQTQQGRPQNLPPLGSFSPPINGRENDPQLNLPAVQPPAMEFASQQVPHHPSHVVPQVIPGGTLPLSDLRGAMDGNFAPQPLQSQDVQFIEPTSRPEIDLDPSLVPVIPEPIKPLENDLTLIEETSFFDRAKKYMGNKQVYTEFLKILNLFSQDLIGTEELVDKVEHYLGGSPELFDWFKSFVNYVEKPKHIENVIHEKHRLDLDLCEACFPSYKKLPKADTFMPCSGRDEMCWEVLNDEWVGHPVWASEDSGFIAHRKNQYEETLFKIEEERHEYDYYIEANLRTIQLLETIANKISNMTPEEKQAFKLPPGLGHTSMTIYKKVIRKVYDKDRGFEIIDALHENPAVSVPIILKRLKQKDEEWRRAQREWNKVWRELEQKVFYKSLDHLGLTFKQADKKLLTAKQLISEISSIKVDQTNKRIYPFTPKPKSQLDYDLKDKGVLFDILCLVNNFIDHSSTYSNPDKEKLAAFFKGFLSVFFSISYQEIENSTPADSDAVSNSNPRKRLWESEQPLKELLRSKHKHHKKRDTSAEDDSISDDQNSPEKSPQEIEDEEVIRQEAKKPWLLGNILDEANDHGYVSNRKIFNLFANTNVYVFFRHLTTMYQRLSEVKNMNEEVTREINSRNVVQFAKDLNLVSTQLSDMGLDFKGKDAYDQLLTLCKRLIRNDMDHQWFEESLRQAYKNKAFKIYTVDKVIQSLVKHAHSIITDAKSAEIMILFEKDRRCTATSTKEQILYRLQVRSYMGLTENMFRVEINKDSCHVCIQYVAIDDLTLKEPESLKDRWHYYLTSYALSHPTEGIPHEEIKVPFLEKTLQDDEEADEGSEERSPSGHSLSNLRIQVNPESYELQIEPGSKDIFTRFAVNRFLNSSENNSNTDLKEEKRKTLMSQLDGSRGWKKGMSQELVDKTLTALNYVRLHGKHESLNKNPALVQGTRDPRHESSVAKEASSLESHADDTELKAGETTAEDIQGNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEQ0</accession>
    <name>C5DEQ0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0C11110p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0C11110g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552699.1">
        <property type="nucleotide sequence ID" value="XM_002552653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEQ0"/>
    <dbReference type="FunCoup" id="C5DEQ0">
        <property type="interactions" value="211"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEQ0"/>
    <dbReference type="GeneID" id="8291584"/>
    <dbReference type="KEGG" id="lth:KLTH0C11110g"/>
    <dbReference type="eggNOG" id="KOG1674">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061246_1_0_1"/>
    <dbReference type="InParanoid" id="C5DEQ0"/>
    <dbReference type="OMA" id="NDSHNIH"/>
    <dbReference type="OrthoDB" id="337735at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20558">
        <property type="entry name" value="CYCLIN_ScPCL7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000085">
        <property type="entry name" value="Pho80p cyclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013922">
        <property type="entry name" value="Cyclin_PHO80-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615:SF117">
        <property type="entry name" value="PHO85 CYCLIN PHO80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08613">
        <property type="entry name" value="Cyclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="251"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="267"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="280" mass="31209" checksum="3755A0EC2D66E0E8" modified="2009-07-28" version="1">MSDKESGGASAKHPDLTEDTKPDTGEDPVPEVALPNDFSQCPRTDLVILISRMLSFLIRINDSIPKDNSSELTRFHSRVPPQISVYDYLMRLTRYSSLEPAVLIASVYYIDLLSAMYTAFSLNSLTVHRFLLTATTVGSKGLCDSFCTNSHYAKVGGVQCSELHVLESEFLKRVNYRILPRDDNILNCKAERREGLFVTATYAKENRGPNNGFNVLDTYYQKIIEIVGSYDSSPDKTKRAHYVMKTPPAPREAEAPKKKRAHEESESAGSQASSSCKKPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>C5DF59</accession>
    <name>C5DF59_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Pre-mRNA-splicing factor CEF1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0D12452g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CEF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553252.1">
        <property type="nucleotide sequence ID" value="XM_002553206.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DF59"/>
    <dbReference type="FunCoup" id="C5DF59">
        <property type="interactions" value="161"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DF59"/>
    <dbReference type="GeneID" id="8295492"/>
    <dbReference type="KEGG" id="lth:KLTH0D12452g"/>
    <dbReference type="eggNOG" id="KOG0050">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009082_2_1_1"/>
    <dbReference type="InParanoid" id="C5DF59"/>
    <dbReference type="OMA" id="KYGTHQW"/>
    <dbReference type="OrthoDB" id="1410009at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11659">
        <property type="entry name" value="SANT_CDC5_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047242">
        <property type="entry name" value="CDC5L/Cef1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021786">
        <property type="entry name" value="Cdc5p/Cef1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047240">
        <property type="entry name" value="SANT_CDC5L_II"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45885">
        <property type="entry name" value="CELL DIVISION CYCLE 5-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45885:SF1">
        <property type="entry name" value="CELL DIVISION CYCLE 5-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11831">
        <property type="entry name" value="Myb_Cef"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="HTH myb-type" evidence="11">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="10">
        <location>
            <begin position="9"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="10">
        <location>
            <begin position="57"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="11">
        <location>
            <begin position="65"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="240"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="340"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="244"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR22814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="563" mass="64135" checksum="C4555982BA84A9B8" modified="2009-07-28" version="1">MPPVPIYVKGGVWSNIEDQILKAAIQKYGTHQWSKVASLLQKKTAKQCESRWNEFLNPNLNFSEFSKEDDSRLLELVKKLPNQWRTISEAMGRTAQACINRYNTLLEGEDSELKLGSSLDFEVGNIDLAAESRPARPDPADLDSMDKEMLAEARARLLNTQGKKATRKIRERMLEESKRVAHLQKRRELKQAGIDSKIKAPRKKYGTEIDYNADVVYEREPLSGPYDTSEEDKRAARAITNFERSVDRSGLKDDARPGKSARPNKKRGRDEKTQISGDDSIVTNEYKKPKLELPIPALASQGKAVGAGTEHRTKPSKKAILRLFQQLPEPKNDFEIVLDDESEDEETEQTNSPDIESENLSREVYEEMSASLESRLPEQQVSTDSPNYKKFQSSRAALPLPDPIISANTPIDIEFNALLASTLIGHEPIEGYEPIEGQETLIRTKNALFEALEEPQEQERAEYEQRVRRILSKSPYDNPDATQTSIEDALSRLKTLQTSMAEANLLKDQNTSTANKIFTKLLPKLSHLRQKYYLHYKMYQNEYYAIQARKKKLELFLEGAKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>C5DF88</accession>
    <name>C5DF88_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0D13134p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0D13134g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553281.1">
        <property type="nucleotide sequence ID" value="XM_002553235.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DF88"/>
    <dbReference type="GeneID" id="8295523"/>
    <dbReference type="KEGG" id="lth:KLTH0D13134g"/>
    <dbReference type="eggNOG" id="ENOG502SFEA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_700338_0_0_1"/>
    <dbReference type="InParanoid" id="C5DF88"/>
    <dbReference type="OrthoDB" id="4036433at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002948707" evidence="1">
        <location>
            <begin position="23"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="394" mass="44293" checksum="F5FB330019180617" modified="2009-07-28" version="1">MKPWILECFSLLAYANIRFILCSNYETLNSIAARDSKKQSSRFGLELEPGCMSKCLIAPLMKDVQQNAYVLGRLDNHTELYPGVSSCLLDIEKKNSLGQYRSEEAIVFEYYACIINVELRVLADLREQRKLHQYPLKGNWLAKDVSILANATKCLVIVAILIAFRRYIELSFLKVVEELRSQSCLLMTALDLRINALASNVSNFGTSMGTKLQKLRKRVKVGVQSSLETKTVSILSKENMGNTEKFPAIEKNCVGRQAKVVDEFSICSHEPGGDEPSGDFGLPLQRSEQLPLELASKKKGLLFDLTTKKGRNDWKEYINRDIRRIAAHQINAAGQSAIVKSSVDCNSGGSKENDVLDDTLQNVPLYDRHGKPLRRICVPGIGWISRKKYLEQRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFB9</accession>
    <name>C5DFB9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Probable transporter MCH1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0D13838g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553312.1">
        <property type="nucleotide sequence ID" value="XM_002553266.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFB9"/>
    <dbReference type="FunCoup" id="C5DFB9">
        <property type="interactions" value="36"/>
    </dbReference>
    <dbReference type="GeneID" id="8295554"/>
    <dbReference type="KEGG" id="lth:KLTH0D13838g"/>
    <dbReference type="eggNOG" id="ENOG502QTNE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012596_3_0_1"/>
    <dbReference type="InParanoid" id="C5DFB9"/>
    <dbReference type="OMA" id="PTMWWLA"/>
    <dbReference type="OrthoDB" id="199930at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21576:SF45">
        <property type="entry name" value="TRANSPORTER MCH1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21576">
        <property type="entry name" value="UNCHARACTERIZED NODULIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="4" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="30"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="64"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="121"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="197"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="257"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="296"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="327"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="352"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="383"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="427"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR22874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="457" mass="50652" checksum="642E3EA41E4BF9BC" modified="2009-07-28" version="1">MTLSTVEHWLTFHVRSCLKSKFHITTLRKVAFVIALISCVASGLVTIISLFTEPWRQHKHFTSLQINLISSAVNLGGYLTPPLLGILSDSHGPVILSWMAVLGFVPSYAYSSYAFDHPGAHFAFTLVAFAAIGVATSALFFSGLLTCAKLYPRYKFLSISCPTTFYGLSSLLASEVLKHPWFSRTYDHLDLGRVFRSFAWFYAVVGTLTWVSTSIVAMLKEPVEQEEHEPLLPTDSRVPPAQDYVERMRRFVRDPSAYLMMLAFFLTIGALEMFLANMGSLAALVAPNNRDIQARVLSYYALCSTLTRLCVGLLADFFTKYNLSRMWILYGMLLIGLISQILVPNIGRSKALVTLSSALSGTAYGGLFTIFPTLTLSVWGDAVFGTAYGCFMVAPAIGSTVYGIVYARIYDTQCSVENPSSTCIRPAFYVTSISFFVALLTSITIYVGFWKRRAVEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFP8</accession>
    <name>C5DFP8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0D16896p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0D16896g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR23003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553441.1">
        <property type="nucleotide sequence ID" value="XM_002553395.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFP8"/>
    <dbReference type="FunCoup" id="C5DFP8">
        <property type="interactions" value="172"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFP8"/>
    <dbReference type="GeneID" id="8295690"/>
    <dbReference type="KEGG" id="lth:KLTH0D16896g"/>
    <dbReference type="eggNOG" id="KOG2056">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040577_1_0_1"/>
    <dbReference type="InParanoid" id="C5DFP8"/>
    <dbReference type="OMA" id="QGTYNFR"/>
    <dbReference type="OrthoDB" id="4033880at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030125">
        <property type="term" value="C:clathrin vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16992">
        <property type="entry name" value="ENTH_Ent3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000006">
        <property type="entry name" value="Clathrin interactor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276:SF45">
        <property type="entry name" value="CLATHRIN INTERACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276">
        <property type="entry name" value="EPSIN/ENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01417">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="2">
        <location>
            <begin position="24"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="234"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="339"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="251"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="341"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="368"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="384"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR23003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="401" mass="43878" checksum="ACD62D2494F73723" modified="2009-07-28" version="1">MSLENGLSNLTLYDAKKYFRKAQNMVFNYTEMEAKVREATNNEPWGASSTLMEQISQGTYNYREREEILGMIFRRFTEKTASEWRQIYKALQLLDYLIRHGAERFIDDTRANLSLIKMLESFHYIDSQGRDQGINVRNRAHELVLLLDDDGKIRTERRKARETAKKYKGVAGGAPSSYLPASAVNRTAGFGRSTNNGISVSADYDSEEEDYQNGNSVQNGHDSNARAAFKDLSPAVSGLETSKPQDNGGDRDDDDDDDFTDFQSSAPVAQAALKTTASTLADLYSGIPTQPAAMGASPSMPVPQTSTPAPAPHVNVAQKKPDPFAGLFTSAKVHSNDNIKTKVASNTPKASTPASNDNTTTASYAEKEGEDDEDDDGFGEMTQAAPSTATTNAQEVDLLSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFX9</accession>
    <name>C5DFX9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA damage checkpoint control protein RAD17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0D00814g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the checkpoint clamp complex involved in the surveillance mechanism that allows the DNA repair pathways to act to restore the integrity of the DNA prior to DNA synthesis or separation of the replicated chromosomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the rad1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552759.1">
        <property type="nucleotide sequence ID" value="XM_002552713.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFX9"/>
    <dbReference type="FunCoup" id="C5DFX9">
        <property type="interactions" value="199"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFX9"/>
    <dbReference type="GeneID" id="8294979"/>
    <dbReference type="KEGG" id="lth:KLTH0D00814g"/>
    <dbReference type="eggNOG" id="KOG3194">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057555_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFX9"/>
    <dbReference type="OMA" id="VHLEHIT"/>
    <dbReference type="OrthoDB" id="337581at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030896">
        <property type="term" value="C:checkpoint clamp complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.70.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046938">
        <property type="entry name" value="DNA_clamp_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016587">
        <property type="entry name" value="Rad17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003021">
        <property type="entry name" value="Rad1_Rec1_Rad17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10870">
        <property type="entry name" value="CELL CYCLE CHECKPOINT PROTEIN RAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10870:SF0">
        <property type="entry name" value="CELL CYCLE CHECKPOINT PROTEIN RAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02144">
        <property type="entry name" value="Rad1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF011769">
        <property type="entry name" value="Cell_cycle_RAD17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55979">
        <property type="entry name" value="DNA clamp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4 6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="337"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR011769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR22321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="393" mass="43866" checksum="FEEEBF049446B9D6" modified="2009-07-28" version="1">MLDQNVLFSASTVHLEHITTALNCLTPFGLRDDVLIIIDSDGLSFARENNRVISIQLFLSKELFISYQYNAPESDSDAHTKISVKINHILDSISVASRDKDDVVECTLSYVGEGSPFVLIFEDSAITEKVEYSTYLTKEWDMTGLELDRSLVDLECMMKGDIFYSALQDLKEIGCKECYIYARAGSSGKNIFAIISKSHLGFSKILLPSERSILEKLETFDSDTHEKVHEKPVIGFFDFGSFDKMRASAKIASKVLIRKDAHGLMSVNILSETDDILRGDSKTKSSASPANPHSSAYLSREYPGIVIDISILEKARVDYDDMEEVKLLMQNNDELKAPPAYQPVSTGASRDPNPAAQESTSLLFNERKRHAEELDNGEDGEFIPATNDIPLFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5DG25</accession>
    <name>C5DG25_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ribonuclease P/MRP protein subunit POP5</fullName>
            <ecNumber evidence="4">3.1.26.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0D01870g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. Also a component of RNase MRP, which cleaves pre-rRNA sequences.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic/archaeal RNase P protein component 2 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="4"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552805.1">
        <property type="nucleotide sequence ID" value="XM_002552759.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DG25"/>
    <dbReference type="FunCoup" id="C5DG25">
        <property type="interactions" value="301"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DG25"/>
    <dbReference type="GeneID" id="8295025"/>
    <dbReference type="KEGG" id="lth:KLTH0D01870g"/>
    <dbReference type="eggNOG" id="KOG4639">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086710_1_2_1"/>
    <dbReference type="InParanoid" id="C5DG25"/>
    <dbReference type="OMA" id="IVRCPRA"/>
    <dbReference type="OrthoDB" id="24745at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030681">
        <property type="term" value="C:multimeric ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033204">
        <property type="term" value="F:ribonuclease P RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000460">
        <property type="term" value="P:maturation of 5.8S rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3250:FF:000004">
        <property type="entry name" value="Ribonuclease P/MRP protein subunit POP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3250">
        <property type="entry name" value="Ribonuclease P, Pop5 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002759">
        <property type="entry name" value="Pop5/Rpp14/Rnp2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038085">
        <property type="entry name" value="Rnp2-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15441">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15441:SF2">
        <property type="entry name" value="RIBONUCLEASE P_MRP PROTEIN SUBUNIT POP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01900">
        <property type="entry name" value="RNase_P_Rpp14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160350">
        <property type="entry name" value="Rnp2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00055200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR22367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="164" mass="19030" checksum="B897F928C9DD8CA2" modified="2009-07-28" version="1">MVRLKSRYVLFEVLYPEGRTLTSENNVNNLFKREVLLRHHQVTPARVTVKTILQELRRVIQFNFGDYGSGKANSLLQIKYFSNRTSTGIIRCSREDYEIVVIALTLMNKIDELEGVLLNPIKVSGTIKRIEQYAVKRSARLLNVIQGQSEIDKFDYISYDENED</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DG29</accession>
    <name>C5DG29_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0D01958p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0D01958g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the V-ATPase E subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552809.1">
        <property type="nucleotide sequence ID" value="XM_002552763.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DG29"/>
    <dbReference type="FunCoup" id="C5DG29">
        <property type="interactions" value="804"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DG29"/>
    <dbReference type="GeneID" id="8295029"/>
    <dbReference type="KEGG" id="lth:KLTH0D01958g"/>
    <dbReference type="eggNOG" id="KOG1664">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073641_0_0_1"/>
    <dbReference type="InParanoid" id="C5DG29"/>
    <dbReference type="OMA" id="QHMMAFI"/>
    <dbReference type="OrthoDB" id="10263003at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033178">
        <property type="term" value="C:proton-transporting two-sector ATPase complex, catalytic domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2320.30">
        <property type="entry name" value="ATP synthase, E subunit, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00311">
        <property type="entry name" value="ATP_synth_E_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038495">
        <property type="entry name" value="ATPase_E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002842">
        <property type="entry name" value="ATPase_V1_Esu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45715">
        <property type="entry name" value="ATPASE H+-TRANSPORTING V1 SUBUNIT E1A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01991">
        <property type="entry name" value="vATP-synt_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160527">
        <property type="entry name" value="V-type ATPase subunit E-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="100"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR22371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="230" mass="25900" checksum="9123F9BE4058274D" modified="2009-07-28" version="1">MSTAITSLTPSQVNDELNKMQAFIKKEAEEKSKEIMLKADQEYEIEKTALVRNETSNIDAAMEEKTKKATLKQQITKSTIANKMRLKVLSTREQMLDDIFESAKAELKKLSSDKKKYESVLKSAILESLLRLLEPSVVVKVREQDKQLVNSFKEAVLKEYKAKSGREASLTVSSEYLSKDSAGGVIASDESGKIVVDNTLEERLEILNQEALPALRLELFGISESRKFFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGF9</accession>
    <name>C5DGF9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0D04994p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0D04994g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552939.1">
        <property type="nucleotide sequence ID" value="XM_002552893.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGF9"/>
    <dbReference type="FunCoup" id="C5DGF9">
        <property type="interactions" value="41"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGF9"/>
    <dbReference type="GeneID" id="8295166"/>
    <dbReference type="KEGG" id="lth:KLTH0D04994g"/>
    <dbReference type="eggNOG" id="KOG1477">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026177_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGF9"/>
    <dbReference type="OMA" id="FIKDRGI"/>
    <dbReference type="OrthoDB" id="258495at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050618">
        <property type="entry name" value="Ubq-SigPath_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12864">
        <property type="entry name" value="RAN BINDING PROTEIN 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="35"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="SPRY" evidence="3">
        <location>
            <begin position="212"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="448"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="453"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="484"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR22501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="507" mass="57422" checksum="E068E927DA521CFB" modified="2009-07-28" version="1">MQKLSAYSPTGFQISGDPLFDPPIGDHQDGEAISLVFLISLSVTFAVLMLMLIIVAVYVTFCSTDESEYDEEVASALPNLFKKKRSGVLLDGSFLTPGQYDDEQALLEQEAQELPRMSTFEVELYQRCREFQKMCPPIVKEFGTYTNINDKQFIKDRGIQSYYFLPSINDNVDQYGNFLPSFIVQDKLDVTFTKFNRSSSAVMNYPLPHNKKDAVYFEVKVFKYPAKSNSIFSCGLVTCPYPYFRMPGMAQFSIAYESTGKLRMNNPFYANTLLPKLQEGDVIGFGYRYKTGTILITHNGKKLMDLTHNVGIDLFIGIGAMNAAYTRSYTREGLMEDCDNVSLRERILASDMDQKAPRAIHEQLENVHDSHDEDLASDEIELQVNLGQLGFVYVEANVKKYAFGSVYGEIGVPPAYNGDEIKKDVVLQKGEDLPPMYPTEELAPLAETTVTIREGEPSSKDLEEYENRSSTFDREDGAEEVRPLKPRSNKKKNRKKNRKRGAKRGFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGJ2</accession>
    <name>C5DGJ2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0D05742p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0D05742g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552972.1">
        <property type="nucleotide sequence ID" value="XM_002552926.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGJ2"/>
    <dbReference type="FunCoup" id="C5DGJ2">
        <property type="interactions" value="425"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGJ2"/>
    <dbReference type="MEROPS" id="S33.993"/>
    <dbReference type="GeneID" id="8295200"/>
    <dbReference type="KEGG" id="lth:KLTH0D05742g"/>
    <dbReference type="eggNOG" id="KOG1455">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026209_7_2_1"/>
    <dbReference type="InParanoid" id="C5DGJ2"/>
    <dbReference type="OMA" id="QNAQNLW"/>
    <dbReference type="OrthoDB" id="10249433at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051044">
        <property type="entry name" value="MAG_DAG_Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11614">
        <property type="entry name" value="PHOSPHOLIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="1">
        <location>
            <begin position="50"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="313" mass="35538" checksum="CE6A8C1AD68A8ECB" modified="2009-07-28" version="1">MFFFSGKKSAPIDFPYKPTSSSFIKDKHQFDGADFIYVKWFVATEQTLTAKARLLVVHGFDEYTLLYSRLMDQLSKVGIESFAFDQRGSGETSPGKQRGRTNEYHTFNDLDHFIEWNLEDKDPETPLFLFGHSMGGGIVLNYGCAGRFRDQIAGIVCTGPLIELHPHSAPSRLVTALSPLLAACLPNFRIDTGLDIDATTSDERYRNFLSRDPLTVPLYGSLRQIYDFLARGKKLLEDKEYVAKLQKPVLIFHGISDTINDPKASEKFNKLCTATDKRLELVPGARHSLCLETDEVFERMFNDMHNWLLEHAP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-06-28" modified="2025-06-18" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGT3</accession>
    <name>BLI1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName>Biogenesis of lysosome-related organelles complex 1 subunit BLI1</fullName>
            <shortName>BLOC-1 subunit BLI1</shortName>
        </recommendedName>
        <alternativeName>
            <fullName>BLOC-1 interactor 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">BLI1</name>
        <name type="ordered locus">KLTH0D08052g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the biogenesis of lysosome-related organelles complex-1 (BLOC-1) involved in endosomal cargo sorting.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the biogenesis of lysosome-related organelles complex-1 (BLOC-1).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the BLI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553063.1">
        <property type="nucleotide sequence ID" value="XM_002553017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGT3"/>
    <dbReference type="SMR" id="C5DGT3"/>
    <dbReference type="FunCoup" id="C5DGT3">
        <property type="interactions" value="48"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGT3"/>
    <dbReference type="GeneID" id="8295296"/>
    <dbReference type="KEGG" id="lth:KLTH0D08052g"/>
    <dbReference type="eggNOG" id="ENOG502S8B7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_168467_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGT3"/>
    <dbReference type="OMA" id="AVANHEW"/>
    <dbReference type="OrthoDB" id="4059150at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020491">
        <property type="entry name" value="BLI1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17324">
        <property type="entry name" value="BLI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0175">Coiled coil</keyword>
    <keyword id="KW-0967">Endosome</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000410615" description="Biogenesis of lysosome-related organelles complex 1 subunit BLI1">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="75"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="113" mass="13187" checksum="78EC6E8CCC6755F3" modified="2009-07-28" version="1">MTWKAGMKERVLRQHIEGCIDQVQEYVDLESAQAISSFESKAVDHFRCLDGLKAQYNTREDKISEFKRLQSEYATKVDGLEKSIEHFEKLSEEIEEFLVELEVKTKLASKRQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGT8</accession>
    <name>C5DGT8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 16">Peroxisome assembly protein 12</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14 16">Peroxin-12</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">KLTH0D08184g</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Component of a retrotranslocation channel required for peroxisome organization by mediating export of the PEX5 receptor from peroxisomes to the cytosol, thereby promoting PEX5 recycling.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Component of the PEX2-PEX10-PEX12 retrotranslocation channel, composed of PEX2, PEX10 and PEX12.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the pex2/pex10/pex12 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553068.1">
        <property type="nucleotide sequence ID" value="XM_002553022.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGT8"/>
    <dbReference type="FunCoup" id="C5DGT8">
        <property type="interactions" value="569"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGT8"/>
    <dbReference type="GeneID" id="8295302"/>
    <dbReference type="KEGG" id="lth:KLTH0D08184g"/>
    <dbReference type="eggNOG" id="KOG0826">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031067_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGT8"/>
    <dbReference type="OMA" id="VYCWKCI"/>
    <dbReference type="OrthoDB" id="107372at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990429">
        <property type="term" value="C:peroxisomal importomer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016562">
        <property type="term" value="P:protein import into peroxisome matrix, receptor recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006513">
        <property type="term" value="P:protein monoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017375">
        <property type="entry name" value="PEX12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006845">
        <property type="entry name" value="Pex_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12888:SF0">
        <property type="entry name" value="PEROXISOME ASSEMBLY PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12888">
        <property type="entry name" value="PEROXISOME ASSEMBLY PROTEIN 12 PEROXIN-12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04757">
        <property type="entry name" value="Pex2_Pex12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038074">
        <property type="entry name" value="Peroxisome_assembly_p12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 16" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Pex N-terminal" evidence="17">
        <location>
            <begin position="29"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR038074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAR22630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="384" mass="43909" checksum="7B1C742F0E9B3B7C" modified="2009-07-28" version="1">MDFYSNLPAASSSIASSIFPTLFEVLSADEIDELLTPSVRYIVANLVARNPNKLTIRFSNWFDEWFVLAAKLTLESYYLKTWDATFIEVFYGLKRINASDAVLLRTLQSNPPVEIPMRLTRFQRRAVLCEKVLAPYVALKLDLLHSKLLARSLLQHPPAQEMGQRVRESLRTLYLRFYPLLKKLLFVLNLAVKLYFLSGRTGSTSLLDLILNIQYTRLSKYDYSRNDRSSTAPGQAVRSRPQRLNRSALLFLCRASFSKIKNLLKLSVSQMLPAFIFLLRVFQWWSSQDLSGEIQRRLNNIDKDIPPPPGRFEKNTEGTCRICNGPIRNPAVIGTGYVFCYPCILDYLPQHEGKCPVTGSQLLGCQYDKDSGHWSIKGVRKLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGU7</accession>
    <name>C5DGU7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Translationally-controlled tumor protein homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0D08426g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in protein synthesis. Involved in microtubule stabilization.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TCTP family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553077.1">
        <property type="nucleotide sequence ID" value="XM_002553031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGU7"/>
    <dbReference type="FunCoup" id="C5DGU7">
        <property type="interactions" value="921"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGU7"/>
    <dbReference type="GeneID" id="8295313"/>
    <dbReference type="KEGG" id="lth:KLTH0D08426g"/>
    <dbReference type="eggNOG" id="KOG1727">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095877_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGU7"/>
    <dbReference type="OMA" id="CAMITEG"/>
    <dbReference type="OrthoDB" id="10248936at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.150.10:FF:000002">
        <property type="entry name" value="Translationally-controlled tumor protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.10">
        <property type="entry name" value="Metal Binding Protein, Guanine Nucleotide Exchange Factor, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011323">
        <property type="entry name" value="Mss4/transl-control_tumour"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034737">
        <property type="entry name" value="TCTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018103">
        <property type="entry name" value="Translation_control_tumour_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018105">
        <property type="entry name" value="Translational_control_tumour_p"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11991">
        <property type="entry name" value="TRANSLATIONALLY CONTROLLED TUMOR PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11991:SF0">
        <property type="entry name" value="TRANSLATIONALLY-CONTROLLED TUMOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00838">
        <property type="entry name" value="TCTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01653">
        <property type="entry name" value="TCTPROTEIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="Mss4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01002">
        <property type="entry name" value="TCTP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01003">
        <property type="entry name" value="TCTP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51797">
        <property type="entry name" value="TCTP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TCTP" evidence="7">
        <location>
            <begin position="1"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR22639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="167" mass="18627" checksum="876E419724B46037" modified="2009-07-28" version="1">MIIYKDIISGDELLSDAYDVKLVDGVIYEADCDMVKVGGDNIDIGANPSTEGGDDDVEDGSEVVNNVVHSFRLQQTAFDKKSFLTYIKGYMKSIKNKLQETNPDEVATFEKGAMVYVKKVVGSFKDWEFFTGESMDPDGMLVLLNYREDGTTPFVAIWKHGVSEEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGY1</accession>
    <name>C5DGY1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein AF-9 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0D09196g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553111.1">
        <property type="nucleotide sequence ID" value="XM_002553065.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGY1"/>
    <dbReference type="FunCoup" id="C5DGY1">
        <property type="interactions" value="786"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGY1"/>
    <dbReference type="GeneID" id="8295348"/>
    <dbReference type="KEGG" id="lth:KLTH0D09196g"/>
    <dbReference type="eggNOG" id="KOG3149">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051385_2_1_1"/>
    <dbReference type="InParanoid" id="C5DGY1"/>
    <dbReference type="OMA" id="VKPYHNE"/>
    <dbReference type="OrthoDB" id="16041at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16908">
        <property type="entry name" value="YEATS_Yaf9_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1970">
        <property type="entry name" value="YEATS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038704">
        <property type="entry name" value="YEAST_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005033">
        <property type="entry name" value="YEATS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055129">
        <property type="entry name" value="YEATS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47573">
        <property type="entry name" value="PROTEIN AF-9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47573:SF1">
        <property type="entry name" value="PROTEIN AF-9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03366">
        <property type="entry name" value="YEATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51037">
        <property type="entry name" value="YEATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="YEATS" evidence="7">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="161"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR22673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="188" mass="21598" checksum="EFB49AE161765842" modified="2009-07-28" version="1">MGEVKPPNAPVEHSHMWTIFVRGPNGEDLSDIIKSVVFKLHETYPNPTRAVEAPPFELTETGWGEFEINIKIHFIESANEKPISFYHHLRLHEYQNQNSNGVKLENGGNSPEVESVFYDEIVFNEPNEEFFKVLIAKPGYLLPSNKSEKAVFSKQLEQEEIDRISDGAKKVDEEIEKLKEELTQMIQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DH85</accession>
    <name>C5DH85_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosomal lysine N-methyltransferase 4</fullName>
            <ecNumber evidence="1">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0E02156g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">S-adenosyl-L-methionine-dependent protein-lysine N-methyltransferase that monomethylates 60S ribosomal protein L42.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. SETD6 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553583.1">
        <property type="nucleotide sequence ID" value="XM_002553537.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DH85"/>
    <dbReference type="FunCoup" id="C5DH85">
        <property type="interactions" value="299"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DH85"/>
    <dbReference type="GeneID" id="8291722"/>
    <dbReference type="KEGG" id="lth:KLTH0E02156g"/>
    <dbReference type="eggNOG" id="KOG1338">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017135_0_0_1"/>
    <dbReference type="InParanoid" id="C5DH85"/>
    <dbReference type="OMA" id="WEGLIIC"/>
    <dbReference type="OrthoDB" id="341421at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1410.10:FF:000007">
        <property type="entry name" value="Ribosomal lysine N-methyltransferase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1410.10">
        <property type="entry name" value="set domain protein methyltransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011383">
        <property type="entry name" value="N-lys_methylase_SETD6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050600">
        <property type="entry name" value="SETD3_SETD6_MTase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271:SF34">
        <property type="entry name" value="N-LYSINE METHYLTRANSFERASE SETD6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271">
        <property type="entry name" value="UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF011771">
        <property type="entry name" value="RMS1_SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SET" evidence="3">
        <location>
            <begin position="27"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="197"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR011771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR23146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="498" mass="57492" checksum="0C8F1A9E71EEE5B4" modified="2009-07-28" version="1">MDTSEFETKTNNFLRWASKDAKLWISDKVKLLGTRDGDQGRYMVATQEIIKGEKLFEVPRGSALNVATLSLSMRDKQVYKKLTTEVGHWEGLVIAILYEFKVMNQNSKWWPYFEVLPEPARLNSLMYWTGAELEYLKPSGVYERVDREGAEEMYARVMKCAEDLKITELTNITWEEFMHVASIIMAYSFDMERPDYEDSDEEVEESDEEEEEEKNTVWNDGYFKSMVPMADMLNSDTHKCNANLTYSPEALIMVAVADIPSGEQIYNNYGEYPNSELLRRYGYVEWSGSKFDCGEMPLETLLKAIEVCLEAPRRLIDKLVEILSHDNDIVEDILEGEPLVMESYDCFLDGQVVPECATLVQILTIALQLPDVQQYDEKTLSGYLQRIIKRAIKTVNSVKITKKCLQVWKTASDARLQDYPSHSFREPSPDHVALTGDSLRRRMAECVLQSEVQSFQNCFSSLEQRSKVIEDQQLLDMVLKRKQTAEKGSQKQQKRLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHB4</accession>
    <name>C5DHB4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0E02926p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0E02926g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553612.1">
        <property type="nucleotide sequence ID" value="XM_002553566.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHB4"/>
    <dbReference type="FunCoup" id="C5DHB4">
        <property type="interactions" value="140"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHB4"/>
    <dbReference type="GeneID" id="8291757"/>
    <dbReference type="KEGG" id="lth:KLTH0E02926g"/>
    <dbReference type="eggNOG" id="KOG1347">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012893_1_2_1"/>
    <dbReference type="InParanoid" id="C5DHB4"/>
    <dbReference type="OMA" id="KISHHHI"/>
    <dbReference type="OrthoDB" id="2126698at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13132">
        <property type="entry name" value="MATE_eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045069">
        <property type="entry name" value="MATE_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="308"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="338"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="372"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="401"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="482"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="524"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="597"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="626"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="50"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="126"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="192"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="663"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="97"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="665"/>
            <end position="683"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR23175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="693" mass="77623" checksum="5790B1E89D4F3A43" modified="2009-07-28" version="1">MATMFSKSLAQVHPKLLTNGIGIGNNHRRISLGFLKPSKRNPLVRKYTRTRRRYGPDQQSFRSLGEDFGSTVARETPLDAVDEEDERHSEDGGELSRTVSLPSRVSETPDLQPEVDWILEEHERRYSSVHASDDEEEEEAPHHEPRNMYSHPYYGNDEEQHQEEVLEYDDFMKRVRQQLIYDVRKRQRSPSFVPVTSRGSVPNVYESTQDEEDYSHESVSYESEAKMLASYAFPLILTFLLEQIFPLVCSLTVGHLGKNELAAVSLASMTTNITLAVFEGISTSLDTLCPQAFGAGNYRGVGIHLQRCIAFSMVVFIPFGFMWHFSEFFLGFVVPDKALVALTSKFLKIATLGAPAYIIFENLKRFLQAQGIFDAGIYVLLICAPLNVVTSYTLVWNSRIGLGYIGAPIAMVLNFWLMLTLMLLYIIFVNGRKCWGGFNKKAFTHWKDLSHLAIPGIIMLEAEDLSYEILTLFSSYFGTTYLAAQSAVSTTAALMYMIPFAVGISSSTRIANFIGAKRPDCARIASELGISCSFLVGIFNFTVLMTGRKVIAHVFSNDELVVKQITKLLPLVAFIEIFDSLNAIAGSCLRGQGMQAIGSIINLVVYYLVAIPLGVLLGWTFNLKLYGLWIGIGTGMFLIGVTEAYLVLSPNWEKILERAEMLKEADDEESDEEFEYSDSDSEPSETTGLLPVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHF1</accession>
    <name>C5DHF1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0E03850p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0E03850g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CND3 (condensin subunit 3) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553649.1">
        <property type="nucleotide sequence ID" value="XM_002553603.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHF1"/>
    <dbReference type="FunCoup" id="C5DHF1">
        <property type="interactions" value="273"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHF1"/>
    <dbReference type="GeneID" id="8291798"/>
    <dbReference type="KEGG" id="lth:KLTH0E03850g"/>
    <dbReference type="eggNOG" id="KOG2025">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004446_1_1_1"/>
    <dbReference type="InParanoid" id="C5DHF1"/>
    <dbReference type="OMA" id="NHQKNFV"/>
    <dbReference type="OrthoDB" id="27187at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000793">
        <property type="term" value="C:condensed chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000796">
        <property type="term" value="C:condensin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027165">
        <property type="entry name" value="CND3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025977">
        <property type="entry name" value="Cnd3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14418:SF5">
        <property type="entry name" value="CONDENSIN COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14418">
        <property type="entry name" value="CONDENSIN COMPLEX SUBUNIT 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12719">
        <property type="entry name" value="Cnd3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nuclear condensin complex subunit 3 C-terminal" evidence="9">
        <location>
            <begin position="570"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="510"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="899"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="18"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="899"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="919"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="953"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="974"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1010"/>
            <end position="1021"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR23212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1021" mass="115821" checksum="74B8BEF91FD7DCA1" modified="2009-07-28" version="1">MKHKQTASGAKQAALPSTMPSDTEPTFENPGESHSTNSKIFRAVADVFQNAQSTYAGHRRHIAVLKRIQAKAVEQGYEEVFNYWFDKMVTLVLPLKRTEPVGDRIVRLVGGFISSIEHELEATDDEYDRKEREEVFARFVDQFVRHMLRGIESRDKNARYRVTQLLAVIMDNIGEIDEELYNLLMWSLQKRVYDKESFVRIQSIFCLTKFQDDESVSEAVDDATSKLMHAIQNDPSAEVRRAAMLNLVNTPNTKKLIMERARDVNPINRRLVYSRVLRGMGSSVFEELNTESLDKLISWGLEDREETVRVACARLVSFDWLNLMDGDIIKLLTRLNVTTSKVCEKAMDAIFKYRNDTVTKIKLPENVWGNLTAETSFLIKAFHFHCTKNHLDDVIDSNFPEASKLSEIIQKYIDLRFVQSGLSAIDEECLDFIIQQLLSVAFNFDYSDEIGRRAMLIVIRNTLASHKLKPSQVEICLKVLEVLSINERDFITMTVEIITDIRDEDIEKQEAEEAKKMPSTNFNGTDEDEDDVESFHSAVDDLVNGNETPEGNSQLLVQEKEVSPEALLACLTMSQYMLELVNSPLNENIMITTLIDTLITPAVRNTQPEVRELGVRTLGLCCLLDVDLAAESLYLLGMCVSKGDASLKNTALKVIVDIFSIHGTKVVDGEGKVDSISLHKIFYKTLKNCELPNCQATVAEGLCKLFLGDVFMDDDLFETLVLSYFSPANSKNEALVQAFAFCLPVYCFSHVRHQRRMVRVAGDVLLRLSVLWDDIQTNEDESLPASSMLKPSVIFQELIEWTDSTKLVNKSSELDQKEDTQLDFLLSVLKVYYKLERKDLKKMILTNIGRFNFEDDNVSKWREAHDILEDILDNDDIDSATRNAVGKFTSLIKDFLATREESEQDITKNEASEDDEETSNILHTGASTHSDGNPQAAESTDIIDGNQLSDLHLPGEKTDNTEEHLSLSNRKRKRLEERGCSERPSDPERSSRMVSFVLPEDHSMSSGSSDDCKSDDEYNGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHL2</accession>
    <name>C5DHL2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0E05280p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0E05280g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553710.1">
        <property type="nucleotide sequence ID" value="XM_002553664.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHL2"/>
    <dbReference type="FunCoup" id="C5DHL2">
        <property type="interactions" value="51"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHL2"/>
    <dbReference type="GeneID" id="8291863"/>
    <dbReference type="KEGG" id="lth:KLTH0E05280g"/>
    <dbReference type="eggNOG" id="ENOG502S5C0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1050442_0_0_1"/>
    <dbReference type="InParanoid" id="C5DHL2"/>
    <dbReference type="OMA" id="HINSKDY"/>
    <dbReference type="OrthoDB" id="9985059at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046756">
        <property type="entry name" value="VAS1/VOA1_TM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20520">
        <property type="entry name" value="Ac45-VOA1_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950484" evidence="6">
        <location>
            <begin position="20"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="210"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="V-type proton ATPase subunit S1/VOA1 transmembrane" evidence="7">
        <location>
            <begin position="206"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR23273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="253" mass="28023" checksum="9137E05C06CD9F6C" modified="2009-07-28" version="1">MLLKSLFLFLAALFQACAAETSFLSVNSRRYRDALGDDQVGSLEELTKISDAEEPMVWFKFDKFDLLGELARSTAETRFLTAFFEGSVTDVLDQDSSSKAAENVRSEKFEVAEIPASAFESLYDRVHPSDGALVFEFTESNYDLALLDEYLEAVYIYLEETLANIDNLAFQVPTSGKNYARAHSKVEVTDPEKKPGSSTDGVQSALWTEGLISCLIVAALLLWILVIAISWITSLDISYGALEKPANPLKKTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHL9</accession>
    <name>C5DHL9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0E05434p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0E05434g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553717.1">
        <property type="nucleotide sequence ID" value="XM_002553671.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DHL9">
        <property type="interactions" value="267"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHL9"/>
    <dbReference type="GeneID" id="8291870"/>
    <dbReference type="KEGG" id="lth:KLTH0E05434g"/>
    <dbReference type="eggNOG" id="KOG1910">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_228568_0_0_1"/>
    <dbReference type="InParanoid" id="C5DHL9"/>
    <dbReference type="OMA" id="EYHFRFY"/>
    <dbReference type="OrthoDB" id="1562405at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019441">
        <property type="entry name" value="FMP27/BLTP2/Hobbit_GFWDK_RBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019415">
        <property type="entry name" value="FMP27_SW_RBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019449">
        <property type="entry name" value="FMP27_WPPW_RBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045167">
        <property type="entry name" value="Hobbit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15678">
        <property type="entry name" value="ANTIGEN MLAA-22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15678:SF6">
        <property type="entry name" value="BRIDGE-LIKE LIPID TRANSFER PROTEIN FAMILY MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10344">
        <property type="entry name" value="Hobbit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01214">
        <property type="entry name" value="Fmp27_GFWDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01215">
        <property type="entry name" value="Fmp27_SW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01216">
        <property type="entry name" value="Fmp27_WPPW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="FMP27 SW motif-containing RBG unit" evidence="5">
        <location>
            <begin position="915"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="domain" description="FMP27/BLTP2/Hobbit GFWDK motif-containing RBG unit" evidence="4">
        <location>
            <begin position="1027"/>
            <end position="1176"/>
        </location>
    </feature>
    <feature type="domain" description="FMP27 WPPW motif-containing RBG unit" evidence="6">
        <location>
            <begin position="1392"/>
            <end position="1869"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2203"/>
            <end position="2230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2344"/>
            <end position="2397"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1541"/>
            <end position="1568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="2345"/>
            <end position="2367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR23280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="2397" mass="273319" checksum="DB2CA1BE81B0BCA7" modified="2009-07-28" version="1">MHQFLTWLPLASRYAIYAALLLLLIPNVIIHFCICHISQNSALPSRLTLVPWINGVRFLYVTSERYTLWVRRIKLKFKRALEIELEGLSITLKGSQHLTKSNPHAADLKKPLPQIFIKPLWLTLLKFVRPRKLILKDLVLHVPASKRTFKIETLLIECKPLPGKSPSVSVSLVAICLSDSLGQFSAHTIDYASSYSFTRQNTSRGFCFELKNHVQALTIRGLQARLDKSSQTNYEKRAQHTEEPHSSLDLKRFSEVLIALSDLEIRLENLALLLEDSLQFRSSGILFTTKAIDPSHHSMLDLFPAERKLPLNHEITLSINSLTGLVNGQEVLRIPSINCTSASDLTSFLLLRKPPQLKIGLTLTIVDTAITATKEQLGKLWVIYTTAKSSSPKSGTSRWRYWFSQIKCPHFIVKIIMSNFSFTLRLSQSKHFVVRVSNLHAFGHNSMKPNTIVAYERLNVKGMKNTRDYVDNFLKIDRFSLSYFRADEATNIKVHDIPVVVFSKWEFFSKKIITEESNLTSTLRDLKISLEHSEVLAGASNFLDEWTYTLSAFNQKKDPTDAKGLKYSLRLRLKNTSLSVLVANHLKKELDVLGDDKFNLADYTRGASVCLEEAFLDAMPDECKLHIVHAFVYRVMGHPDVYARSDEIANLKDFVISSNSLTNHCFAPALNIKVDVNAIWLYYYLKDIATNYLRPKKLRASSDKVPFNDLCKEWHVEVASLLIEIGLPHSTKLLFDFENAHFSGESLALHLDRLVMFSPSVYREEEIFVPLALINDFTLDIERLVAKRIVSLNAGFMSLKFEYHLRLYKILDNVMTLVKCFKQIRACFESPIGFKRLKPVPEEPKRVFPIDLSLDLLSLNVEEDPFEQELGLVFKVGVSEQQERLEKLRLFYEEIDSFEPNKVLSQEEMKLKYDSRMQKLFENFSTSWINRIRKARLTFFGNPSQTIKLRNAGTFRFRSLDGMNSTVLKVELERLKMKLREPSFSLDKCSEFLYKYGKQIPKDTAFTTLLPLGISLETERWEIRLRDYPLPILFFPSVEISGDITFAERMPQPFSERFVHVPFVPMASLAKYSNIDSVYGASVIRTLNPVKTYMNLRCQIDSTRPTTITWGKALQPGYQSVMIWFDYLTKPPIDPSEKLGFWDKFRLLIHGKFVFEWAGDSHIHLNIKGSHDPYLITDDGAGLSFCWGGSARLAIHESSDPTEFLKITSKHFLLAIRDFTSPQKIEKALMELNGTITWTMGLAFESGNPKNPGMEERSSNFKPHYHINLFNPAFLEGDKTHDSYAGFRSDFIHLFFRVHASSNPEGSNKVFLAPHCMAHFLKWWSLFSTYTSGPIRQGSLFPDLVQNPKKFSKALFTVKYELHLTPLVISHTYRHADSRLNIESDNETAFTSLKGKFETLKLELHQKRVKMMHSDKKLNVSRTVWKFKMNAGEIDCVSADIRFVYTMLGHPSGPRKGGATANIENDVLKSSWFDFEDYNDLNQITFADPIPLNFCEAPLLYSPRISYLRELNEGVPLDYPFGDESVHECTLGHNHPEVTQRELAKKRAREIETQLDQIEQSIEALSSVAMKGSNGSVQKLELLGRQLHEFRHRLHIIHKVLEDLKLSKMPSSSLLSDDIESELSERHSDKTQDELENALAATATIASFRSMRKVTSAFVKSSFDNRFIIHNMLLKVNNKTRDLLLNYAFNVFSRKKSSFFQTYKAVALLDELLKNRLWNTSSRAKDSAGFFTLEDNLSNADLLERCDEIIREVFEDGFKTFDNYQIKFVSPQIQVTSEAVPRKCILVTARDIETSIVDVNQLLETNQNVPLDVNSLVETRYCAKLNEAHFLIFDKCEIAIDDGLGFQTNGYGMDTNSSFWPPWLPMEMCYNCESLTDSVFLSRSEMILLFTQPNSLFFQDKTKNFGRSEAKFRIGFPELFLTSTSDQYSSIYAIAVELLHFASSFDNKADKLSRVLLAEEVRNNLDRLDTSIIVNLQKTVKNLYRTRGYLKVHDPKGYEKVAQSIAAEIEATVLELNLLMSAIKKNHDRLKVSNVKGSSQDRLNWQIGADEVTWQLYDEFKNSFVSFELGPACFIRSQAPDGANNNKVSISSLECFNLQKNAVYKQLLGPHDNDTDTEKPMIQILWAMGPAIGGIADLEQLVVLLEPFKFKMDHLTSDILLKYLFPGTNEASTAAQSDIAFDVASIGLSKKNTESSETWKLKSVPSLLPPNPLKKSDTEARNSVSLSRRGTGRDMNEMVTRSSRYFNVRSVVIRKVTMSISYKGSRSLITNVDGLTVKVPTLRYTNKLWSRDEFIATLKKDIIKVVLQHTGYIIGNKFTLHKKENEAKFSKALIKTSVGEADSLRSASSSQHKTPQLRHISSSLKPESSSDGRSGHLSQDQRSGADDVTAFYPASHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHM7</accession>
    <name>C5DHM7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="19 20">CDP-diacylglycerol--inositol 3-phosphatidyltransferase</fullName>
            <ecNumber evidence="5 20">2.7.8.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ordered locus">KLTH0E05610g</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18 20">
            <text>a CDP-1,2-diacyl-sn-glycerol + myo-inositol = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11580"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58332"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="2.7.8.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 20 21">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.11" evidence="5 20"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553725.1">
        <property type="nucleotide sequence ID" value="XM_002553679.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHM7"/>
    <dbReference type="FunCoup" id="C5DHM7">
        <property type="interactions" value="683"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHM7"/>
    <dbReference type="GeneID" id="8291878"/>
    <dbReference type="KEGG" id="lth:KLTH0E05610g"/>
    <dbReference type="eggNOG" id="KOG3240">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067602_0_0_1"/>
    <dbReference type="InParanoid" id="C5DHM7"/>
    <dbReference type="OMA" id="AQTYSEN"/>
    <dbReference type="OrthoDB" id="10251079at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003881">
        <property type="term" value="F:CDP-diacylglycerol-inositol 3-phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006661">
        <property type="term" value="P:phosphatidylinositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1760:FF:000021">
        <property type="entry name" value="CDP-diacylglycerol--inositol 3-phosphatidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048254">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014387">
        <property type="entry name" value="CDP_diag_ino_3_P_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15362:SF4">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--INOSITOL 3-PHOSPHATIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15362">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000848">
        <property type="entry name" value="CDP_diag_ino_3_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 20" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="13 20" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="16" id="KW-0464">Manganese</keyword>
    <keyword evidence="14 20 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15 20" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="17 20" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 20 21" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 22" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="16"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="141"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="172"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00050166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00070582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRNR" id="PIRNR000848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="CAR23288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="221" mass="25074" checksum="00F1F3207900C6B0" modified="2009-07-28" version="1">MAKVTASQVLWYVPNQIGYMRVFTAILSFFLMAKHPIWTTWIYGISCLLDALDGTMARKYNQTSGFGAVLDMVTDRSTTSSLICFLCVLFPRYAPVFQLLISLDLSSHYLHMYATLSTGGASHKTIEKEQWLLYMYYSRRWVLFTVCAFNELFYLALYLYGFPQLKHFDVPLWGRAHIGGVMAAVCLPGYVFKQVANVIQLKRAALLLASKDAEAASEKRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DI24</accession>
    <name>C5DI24_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Nuclear cap-binding protein complex subunit 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">80 kDa nuclear cap-binding protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0E09130g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NCBP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553872.1">
        <property type="nucleotide sequence ID" value="XM_002553826.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DI24"/>
    <dbReference type="FunCoup" id="C5DI24">
        <property type="interactions" value="1115"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DI24"/>
    <dbReference type="GeneID" id="8292035"/>
    <dbReference type="KEGG" id="lth:KLTH0E09130g"/>
    <dbReference type="eggNOG" id="KOG1104">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011380_0_0_1"/>
    <dbReference type="InParanoid" id="C5DI24"/>
    <dbReference type="OMA" id="CAAEGLM"/>
    <dbReference type="OrthoDB" id="10252707at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005846">
        <property type="term" value="C:nuclear cap binding complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000339">
        <property type="term" value="F:RNA cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006370">
        <property type="term" value="P:7-methylguanosine mRNA capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000056">
        <property type="entry name" value="Sto1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027159">
        <property type="entry name" value="CBP80"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015172">
        <property type="entry name" value="MIF4G-like_typ-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015174">
        <property type="entry name" value="MIF4G-like_typ-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003890">
        <property type="entry name" value="MIF4G-like_typ-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12412">
        <property type="entry name" value="CAP BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12412:SF2">
        <property type="entry name" value="NUCLEAR CAP-BINDING PROTEIN SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02854">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09088">
        <property type="entry name" value="MIF4G_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09090">
        <property type="entry name" value="MIF4G_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00543">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0506">mRNA capping</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MIF4G" evidence="13">
        <location>
            <begin position="36"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00074671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR23435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="862" mass="99644" checksum="44105D68232D51D4" modified="2009-07-28" version="1">MSGVKRRRNFDDDDGYRDFRPRMPKRQRVPPVVQLCKEMMPDIRTLGETVKAFEEDIKFLSEAIVNEFGNEEYFRDALLKTFYAVVVEQPHKQPAIALLVMVVNASNPVAGKSILNHFFSKLQQWCDNSIDDGFQVTSNETGPWNKVKLLLRFLSLLSPMILEDELLSLYKKLFELSVGLNNSEPDKRNPLSEAIYSNTLLNVPYLFFFRRENESLRQKVSKLISEVEVQYTMKKTDLSLVQEYNKNFPYEPIQWIQAVLPNVKRVLANDLQQLAELFPDYSHLLKEQAGDQGFNDPLTLPSVDQLKPFSGLDRGLGSVDGMWRVPRYSFRVYLANEIGEFETVVPFTTYAGMLFKDIVIDIVESLEFNRKEVAKQVITLDLFFKPGIFTEPGQSIAQLVALHEENPIITTYKIEDLAIEKILSMIFKLPNVSHPSAYFYTLLVEICQNSPKAIAPVFGRAFRFFYHNLDALDFELKLRYLDWFSIQMSNFNFSWKWNEWEGDALKFGETFFNPKVTFMKNLIKKELRLTSNKSDVEDSLTDVFRPFTDSSFVPSQDLYEYYQSFFKGYEISEDQIKDNSLFFTESSLPFSDLVQQIVNYFHKQPLDRNISELVSILEKMKVDNETIVVDFNRLAVTTLVQVLVFSGNRSISHANKYIGDARTELLEILGKIEASQEQKERWIVEAIIRYWNCNSQNGYLIADTCKNFDLISSKSILDFSFLDDKGRNWGLVDATSIESTFRVLSELSSRRDTSVETFIFVFERLVEIASNVVEELGLSVDSELSASMADPDEMQDESADLQKLDCSWKYEGCMGFVKSVLRKYADEYASALEKLDSIINERVTHQQTKRTLQGWCRELSEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DI40</accession>
    <name>C5DI40_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0E09504p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0E09504g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553888.1">
        <property type="nucleotide sequence ID" value="XM_002553842.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DI40"/>
    <dbReference type="FunCoup" id="C5DI40">
        <property type="interactions" value="371"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DI40"/>
    <dbReference type="GeneID" id="8292052"/>
    <dbReference type="KEGG" id="lth:KLTH0E09504g"/>
    <dbReference type="eggNOG" id="KOG2659">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055870_0_0_1"/>
    <dbReference type="InParanoid" id="C5DI40"/>
    <dbReference type="OMA" id="LWCWCEN"/>
    <dbReference type="OrthoDB" id="2415936at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013144">
        <property type="entry name" value="CRA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024964">
        <property type="entry name" value="CTLH/CRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006595">
        <property type="entry name" value="CTLH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050618">
        <property type="entry name" value="Ubq-SigPath_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12864">
        <property type="entry name" value="RAN BINDING PROTEIN 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10607">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00757">
        <property type="entry name" value="CRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00668">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50897">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CTLH" evidence="1">
        <location>
            <begin position="96"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="385" mass="43363" checksum="E92F37FD40C84705" modified="2009-07-28" version="1">MTNSNGTYDRKAFTRQEWKEYVVDSGLSLDLNGNSSELRSGGDLQNKRSCSEPSIANLLLNYFVVMSYEESSIRMARELGYVRNNKDAELFNQVYKIRERAHVRELIKRGRILEAIELICSTFGAQVLENVKTESSTKPKAGEDDLHFKLLLLNLIEMIRKHHQSGASPEESSGFILELIEYSQQKLAFKADSNKRYMKELELVMTLLLFPMESSSEGESSSSLPKSLRNLYSLSLRTKIADLVNRKLLESMHAEILSSSNSAAFPDLIGFKTFHKGFSTYNGLLNTKTKDSTDIEYTTTKLGDIKGGLDATPASKPWDTNAWKNTASLLKERDSESETKPDDSVEASQQIQYEAKLNQVMKLWAWCENQLHNCGVGVPRVEGGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIA7</accession>
    <name>C5DIA7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0E11022p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0E11022g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the methylenetetrahydrofolate reductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553955.1">
        <property type="nucleotide sequence ID" value="XM_002553909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIA7"/>
    <dbReference type="FunCoup" id="C5DIA7">
        <property type="interactions" value="42"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIA7"/>
    <dbReference type="GeneID" id="8292121"/>
    <dbReference type="KEGG" id="lth:KLTH0E11022g"/>
    <dbReference type="eggNOG" id="KOG0564">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025841_2_1_1"/>
    <dbReference type="InParanoid" id="C5DIA7"/>
    <dbReference type="OMA" id="GLMPINS"/>
    <dbReference type="OrthoDB" id="16284at2759"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004489">
        <property type="term" value="F:methylenetetrahydrofolate reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00537">
        <property type="entry name" value="MTHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.220:FF:000002">
        <property type="entry name" value="Methylenetetrahydrofolate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029041">
        <property type="entry name" value="FAD-linked_oxidoreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004621">
        <property type="entry name" value="Fadh2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003171">
        <property type="entry name" value="Mehydrof_redctse-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053806">
        <property type="entry name" value="MTHFR_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00677">
        <property type="entry name" value="fadh2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45754">
        <property type="entry name" value="METHYLENETETRAHYDROFOLATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45754:SF1">
        <property type="entry name" value="METHYLENETETRAHYDROFOLATE REDUCTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02219">
        <property type="entry name" value="MTHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21895">
        <property type="entry name" value="MTHFR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51730">
        <property type="entry name" value="FAD-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MTHFR SAM-binding regulatory" evidence="9">
        <location>
            <begin position="411"/>
            <end position="657"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF21895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR23518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="660" mass="73979" checksum="5062CBB35761EB2D" modified="2009-07-28" version="1">MHAPRKLNKCGQGTALKLIMSTIKQRYESTDGPFISLEFFPPKTKLGKQNLTARMTRMTALNPLFITITWGAGGTTAKKTLELATVAQRELNVPVCMHLTCTNTNKEIIDEALKLAHEADIRNILALRGDPPVGEEWETPSESSEFQHAVDLVRYIKEKYNDDFCVGVAAYPEGHCEGEADGVAQDPRRDLGFLKEKVDAGADFVITQLFYDVEKFLSFEQMFRNEVSADIPLFPGLMPINSYLLFNRAAKLSHASIPQAIRDRLPQEYHSDDNKVRAIGVDILVEMVEEIYKRTEGRIKGIHFYTLNLEKAIAQIVTKSPSLSKILEEDKEDDLVEGVGEIALVDGNTLEEDENVKKRRRQSSHNDDLTCNRVLLDEAGKSIASGRESSGAPSRKVVISISQGSGALGKDATWDEFTNGRFGDSRSPAFGEIDGYGPSLKVSSKKAYEIWGQPSSTDDIKTLFIRYLEGSLEALPWSDLGLSAETALIQEELIQLNERGYLSLASQPATNSTPSTDKIFGWGPPGGYVYQKSFVEMFVHKQQWELEIKPKLEPLGSKVSYYVGDSAETFYTNLDSSCSSVVTWGVFPNSEVLQTTIVEEESFKAWRDEAFSIWLEWSKLFPRNSRSNTLLKQMHRDYYLVSIVHHDFSDCDALWDLLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIG9</accession>
    <name>C5DIG9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0E12430p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0E12430g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554017.1">
        <property type="nucleotide sequence ID" value="XM_002553971.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIG9"/>
    <dbReference type="FunCoup" id="C5DIG9">
        <property type="interactions" value="490"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIG9"/>
    <dbReference type="GeneID" id="8292185"/>
    <dbReference type="KEGG" id="lth:KLTH0E12430g"/>
    <dbReference type="eggNOG" id="KOG0777">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014015_6_0_1"/>
    <dbReference type="InParanoid" id="C5DIG9"/>
    <dbReference type="OMA" id="ANFAYFW"/>
    <dbReference type="OrthoDB" id="6921389at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004659">
        <property type="term" value="F:prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001">
        <property type="entry name" value="GERANYLGERANYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001:SF44">
        <property type="entry name" value="GERANYLGERANYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00723">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR23580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="326" mass="37245" checksum="CCBB645C20F0B34B" modified="2009-07-28" version="1">MHSSVSLIEELANEAPHWTSEEESNLRRPFDYLTSSPGKNFRAELIQIFNSFYELPDHRIAIISKLVETLHTSSLLIDDIEDSSEWRRGLKAAHLVYGVPMTINTANYMYFYAMECLKELGNGCDDLSLNKLLIIFNEEMMDLHRGQGLDIYWRDCFMVPTEPEYLNMVMNKTGGLFRLTVRLMEVFSPSFSGAKSLVPLSNLLGILYQIRDDFMNLQDAKMIENKGFADDVSEGKLSFPIIHGIKHGSLNGNSLVWDILKQRTHDDALKKRLVSYLEGTSGSMEYTRRKVKDLGVLIKEKYLSSMQDRGYDSSALEKVVDRLCAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIK7</accession>
    <name>C5DIK7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">KLTH0E13288p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0E13288g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554055.1">
        <property type="nucleotide sequence ID" value="XM_002554009.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIK7"/>
    <dbReference type="FunCoup" id="C5DIK7">
        <property type="interactions" value="114"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIK7"/>
    <dbReference type="GeneID" id="8292224"/>
    <dbReference type="KEGG" id="lth:KLTH0E13288g"/>
    <dbReference type="eggNOG" id="KOG0448">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011752_0_0_1"/>
    <dbReference type="InParanoid" id="C5DIK7"/>
    <dbReference type="OMA" id="RCERMIL"/>
    <dbReference type="OrthoDB" id="9984778at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008053">
        <property type="term" value="P:mitochondrial fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051646">
        <property type="term" value="P:mitochondrion localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000638">
        <property type="entry name" value="Transmembrane GTPase Fzo1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027094">
        <property type="entry name" value="Mitofusin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10465:SF0">
        <property type="entry name" value="SARCALUMENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10465">
        <property type="entry name" value="TRANSMEMBRANE GTPASE FZO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51718">
        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Dynamin-type G" evidence="13">
        <location>
            <begin position="179"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="402"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="419"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR23618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="845" mass="95796" checksum="12BA984B3C89A70D" modified="2009-07-28" version="1">MSQRELSPKSHEDHESHFGGDLENEYEDESATVVPAEPRHLTYSRNTHTFADDDSSTVAGDIHHLAVHDSTHRRKHSNDHMLSLHLSQWNYNNNRVGLNRGIQSSEELIFLLMDENEKRPIFVPVGPEAKKLHILELDFKLRGNGRDKVDLDKNAISKLFKARSETTLKHLKNLQRRVDDNSSKVFITGDLNAGKSTFCNALLKRRIMPVDQLPCTNVFCEILEAREYTNVEEVHAIPIDLAPTVKEATEVYDIQNKSTYRVYPIKQLEDLVYRSDLYSLLKVYIKDDKRPAESSLLRNGTVDISLIDSPGLNMDSIQTTEVMSRQEEIDMVIFVVNSENQLTLSGQEFIQVASREKKLMFFVVNKFDQIRDKDRCKKLILDQIKKMSPETHKQSNEFVHFISSGSKPEPSGGGGEPGGDGDGDGDNGEFNNEDPDFDRLETSLRNFVLKKRSLSKLLPAKTYLVKLLQDIATIAKWNLDLYDSENQALKDELKGMNPEIKSTSLQCQRLTDNVDKIVESCVGENYEFTKSKINMALELQPADFPPYEGLLSVYDYVFRARQFIMDQVKLAVDTSEFKAKGDTEKAVNSINTLGKTELGEEFMSDRKFNSDLMFTKKKHVSLKALSVAFSINDLFSPSWEGFISYLTWGVSNDAIQENDLSNSQEKHWLSALGLGNYSPAKYWTNPSLLFTSRIPALAVYSWGGARVLSNIVLYGSRFFTWQSITRASSSVLLVGTVLGAAYLIHDLPRALPLNLSQKYKSKLQELDYAHKNAKRISGEARDVLRFPSREIIKSCESVLGKKQAERREIETKLHDNLASLKFFTSLSQRAASQTKVIEQIALDVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIP8</accession>
    <name>C5DIP8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 12">Vesicular-fusion protein SEC18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">KLTH0E14190g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10 12">Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554096.1">
        <property type="nucleotide sequence ID" value="XM_002554050.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIP8"/>
    <dbReference type="FunCoup" id="C5DIP8">
        <property type="interactions" value="891"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIP8"/>
    <dbReference type="GeneID" id="8292265"/>
    <dbReference type="KEGG" id="lth:KLTH0E14190g"/>
    <dbReference type="eggNOG" id="KOG0741">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008037_2_0_1"/>
    <dbReference type="InParanoid" id="C5DIP8"/>
    <dbReference type="OMA" id="CFDNEIA"/>
    <dbReference type="OrthoDB" id="9982946at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005795">
        <property type="term" value="C:Golgi stack"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
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        <property type="term" value="P:SNARE complex disassembly"/>
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    <dbReference type="CDD" id="cd00009">
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        <property type="entry name" value="RecA-like_NSF-SEC18_r1-like"/>
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        <property type="entry name" value="vesicle-fusing ATPase isoform X1"/>
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        <property type="entry name" value="Vesicle-fusing ATPase protein"/>
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        <property type="entry name" value="Vesicular-fusion ATPase SEC18"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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        <property type="entry name" value="AAA+_ATPase"/>
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        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
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        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
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    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004201">
        <property type="entry name" value="Cdc48_dom2"/>
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    <dbReference type="InterPro" id="IPR029067">
        <property type="entry name" value="CDC48_domain_2-like_sf"/>
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    <dbReference type="InterPro" id="IPR003338">
        <property type="entry name" value="CDC4_N-term_subdom"/>
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    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
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    <dbReference type="InterPro" id="IPR039812">
        <property type="entry name" value="Vesicle-fus_ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23078:SF3">
        <property type="entry name" value="VESICLE-FUSING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23078">
        <property type="entry name" value="VESICULAR-FUSION PROTEIN NSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
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    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF02933">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
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    <dbReference type="SMART" id="SM01072">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01073">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="ADC-like"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF54585">
        <property type="entry name" value="Cdc48 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 12" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="domain" description="CDC48 N-terminal subdomain" evidence="15">
        <location>
            <begin position="33"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="CDC48" evidence="14">
        <location>
            <begin position="138"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="13">
        <location>
            <begin position="278"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="13">
        <location>
            <begin position="561"/>
            <end position="697"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00056429"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00068637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU367045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM01073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAR23659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="763" mass="84273" checksum="4A43262DFC3BB6E6" modified="2009-07-28" version="1">MEKFGLAGKLPSFGRATHNAPPNMSNVDMSPRHLAVANCPNNAFALANVAAVSPSDFPDNIYVLVDNLFVFTTKHSNDVPPGTIGFNGNQRTWGGWSLNQAVQARAFDLFQYSGKHAYLGTLDLEISFRSKGKAVDTQFEQDDLAKQFLKNFESQIFSPTQYLIFEYKGHIFDIKVKGLQTIDLGDVEIVTPISTGIETKGILIKQTQINYFKGRDGLVNLKSGNSLRPRSDAVIRPDFKFEDLGVGGLDKEFTKIFRRAFASRIFPPAVIEKLGISHVKGLLLHGPPGTGKTLIARKIGTMLNAKEPKIVNGPEILSKYVGSSEENIRNLFKDAEAEYKAKGEESSLHIIIFDELDSVFKQRGSRGDGTGVGDNVVNQLLAKMDGVDQLNNILVIGMTNRKDLIDSALLRPGRFEVQVEIQLPDEHGRLQILEIQTKKMRENNMMAPDVNLKELAALTKNFSGAEIEGLVKSASSFAINKTVSIGKGSTKLNPKDIAAMKVTMQEFLSALDEVTPAFGISEEDLKTCVEGGVIPYSTRVEDILKNGQRYVRQVRDSDKSRLVSLLIHGPAGSGKTALAAAIGLKSEFPFIRLISPEEIAGMSESAKIAYIDNTFRDAYKSPLNILVIDSIETLVDWVPIGPRFSNNILQVLKVYLKRKPPKDRRLLLISTTSTYSVLQQMDLLSCFDNEIAVPNITNLDEFNNVMIETDFLDDAGRVQVLNEFSRLGLKLNVGIKKALTNIETARFDENPVNEIVELMAQSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIQ3</accession>
    <name>C5DIQ3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0E14322p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0E14322g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CBF/MAK21 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554101.1">
        <property type="nucleotide sequence ID" value="XM_002554055.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIQ3"/>
    <dbReference type="FunCoup" id="C5DIQ3">
        <property type="interactions" value="1078"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIQ3"/>
    <dbReference type="GeneID" id="8292271"/>
    <dbReference type="KEGG" id="lth:KLTH0E14322g"/>
    <dbReference type="eggNOG" id="KOG2038">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003417_0_0_1"/>
    <dbReference type="InParanoid" id="C5DIQ3"/>
    <dbReference type="OMA" id="EIWCNDE"/>
    <dbReference type="OrthoDB" id="28947at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005612">
        <property type="entry name" value="CCAAT-binding_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040155">
        <property type="entry name" value="CEBPZ/Mak21-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12048">
        <property type="entry name" value="CCAAT-BINDING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12048:SF0">
        <property type="entry name" value="CCAAT_ENHANCER-BINDING PROTEIN ZETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03914">
        <property type="entry name" value="CBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCAAT-binding factor" evidence="3">
        <location>
            <begin position="638"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="128"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="548"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="750"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="938"/>
            <end position="1078"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="11"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="27"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="47"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="66"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="137"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="191"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="548"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="564"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="758"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="943"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="963"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="984"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1040"/>
            <end position="1050"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR23664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1100" mass="121593" checksum="441E5A397B5E4918" modified="2009-07-28" version="1">MSGTGLNLASLKDKVSSKLKENQKQPKNGKKAPKKQSKQRGKDAEKGTNSAATTSSDKQSTPQDGAAKDSADDVLRREALALGASEKDLELVAGLSDDDNASEQEFGNAKQDNDTAFNDEFHKFLKSAGIEGAPVPEDVDDAEVEQVEEEQVEEEQAEEDEEEKEEEKEEEQKEEQIKKPTANVTKAAPAEQEEDKDDEVPQLSDDLSESEQSEVEDADGPFLSQDSEPETEEKKEDKNLVSQTHMVSSDKLLLPSDVTWYQIPLDAEAYQKNDPLPADKVDALFQRGKDALEADNSLYYDEFTKNSSQRKFMSQILSDGTLNDKISALTLLIQESPLHNTKSLDTLVSYCDKKSRNSALQSLGALKDLLLNGLLPDRKLRYFKNQNLSMMLNKKTLALFYFEDYLKNLFFKVLQVMEKLSHDPIIYVRMQIITHIFDLLTAKPEQEFNLLRLGVNKLGDIDKKVSSKTSFQLLKLEQAHPNMKSVILDATVDVALRPSADYHTTYYAVLTLNQTILKRSEEDVANQLIKTYFTLFEKYLLVTDSSNVEKGDHAPKNKDASYEKKRKKNFKRGKKGGKSVQNDKSEKDVIDEKNSKLFSAIITGLNRALPFSNMPATVYETHLDTLFQITHSSNFNTAVQALVLIHQVTARAQLNGDRYYRTLYESLLDARLVTSSKQGIYLNLLYKSLKSDTHVARVEAFVKRILQVCTNWLNVGAISGMLFLLLQLAKTVPQIKNLLTNTPADAEYASDAEEGAEGADASDAASYDPRKRDPKFANADQTSLWEVASFLDHYHPTVQSYAAAFVEGSDEVTKPDLGLYTLAHFLDRFVYRNAKQKPSTRGSSIMQPLGGAHTGALIVRASDAHATGADEVPANTQDWLAKRASEVTPDERFFHQYFSTKQTAIKRSENSKIGAAARGAFDEESDLDEDEVWDALVKSRPEVEDDGESDMSMDDLSMGDMSSSDDESDAEDAAEDAAEDSDSDAAAKPAAADSDPAAASSDDDNADDVFYSFADDSAHDAASGKKRARDADDSDDSADDAALAAAFDGESASDSDSESGPDQTDARGPAQRLSRKKLKSLPVFASASDYAQYLDSDSEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIQ5</accession>
    <name>C5DIQ5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">C-1-tetrahydrofolate synthase, cytoplasmic</fullName>
            <ecNumber evidence="8">1.5.1.5</ecNumber>
            <ecNumber evidence="7">3.5.4.9</ecNumber>
            <ecNumber evidence="6">6.3.4.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="ordered locus">KLTH0E14366g</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>(6R)-5,10-methenyltetrahydrofolate + H2O = (6R)-10-formyltetrahydrofolate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
            <dbReference type="EC" id="3.5.4.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:23701"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="22">
            <dbReference type="Rhea" id="RHEA:23702"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NADP(+) = (6R)-5,10-methenyltetrahydrofolate + NADPH</text>
            <dbReference type="Rhea" id="RHEA:22812"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.5.1.5"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:22813"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="20">
            <dbReference type="Rhea" id="RHEA:22814"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>(6S)-5,6,7,8-tetrahydrofolate + formate + ATP = (6R)-10-formyltetrahydrofolate + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20221"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:20222"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="21">
            <dbReference type="Rhea" id="RHEA:20223"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the formate--tetrahydrofolate ligase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.5" evidence="8"/>
    <dbReference type="EC" id="3.5.4.9" evidence="7"/>
    <dbReference type="EC" id="6.3.4.3" evidence="6"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554103.1">
        <property type="nucleotide sequence ID" value="XM_002554057.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIQ5"/>
    <dbReference type="FunCoup" id="C5DIQ5">
        <property type="interactions" value="1130"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIQ5"/>
    <dbReference type="GeneID" id="8292273"/>
    <dbReference type="KEGG" id="lth:KLTH0E14366g"/>
    <dbReference type="eggNOG" id="KOG4230">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003601_2_0_1"/>
    <dbReference type="InParanoid" id="C5DIQ5"/>
    <dbReference type="OMA" id="IKRVVDC"/>
    <dbReference type="OrthoDB" id="5126881at2759"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004329">
        <property type="term" value="F:formate-tetrahydrofolate ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004477">
        <property type="term" value="F:methenyltetrahydrofolate cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004488">
        <property type="term" value="F:methylenetetrahydrofolate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00477">
        <property type="entry name" value="FTHFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01080">
        <property type="entry name" value="NAD_bind_m-THF_DH_Cyclohyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000006">
        <property type="entry name" value="Bifunctional protein FolD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000245">
        <property type="entry name" value="C-1-tetrahydrofolate synthase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001123">
        <property type="entry name" value="C-1-tetrahydrofolate synthase, cytoplasmic isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10860:FF:000005">
        <property type="entry name" value="C-1-tetrahydrofolate synthase, cytoplasmic, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.410.10:FF:000001">
        <property type="entry name" value="Putative formate--tetrahydrofolate ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.410.10">
        <property type="entry name" value="Formyltetrahydrofolate synthetase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01543">
        <property type="entry name" value="FTHFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01576">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000559">
        <property type="entry name" value="Formate_THF_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020628">
        <property type="entry name" value="Formate_THF_ligase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000672">
        <property type="entry name" value="THF_DH/CycHdrlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020630">
        <property type="entry name" value="THF_DH/CycHdrlase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020867">
        <property type="entry name" value="THF_DH/CycHdrlase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020631">
        <property type="entry name" value="THF_DH/CycHdrlase_NAD-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099">
        <property type="entry name" value="C-1-TETRAHYDROFOLATE SYNTHASE, CYTOPLASMIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099:SF26">
        <property type="entry name" value="C-1-TETRAHYDROFOLATE SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01268">
        <property type="entry name" value="FTHFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00763">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02882">
        <property type="entry name" value="THF_DHG_CYH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00085">
        <property type="entry name" value="THFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00721">
        <property type="entry name" value="FTHFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00722">
        <property type="entry name" value="FTHFS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00766">
        <property type="entry name" value="THF_DHG_CYH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00767">
        <property type="entry name" value="THF_DHG_CYH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0436">Ligase</keyword>
    <keyword evidence="19" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="17" id="KW-0521">NADP</keyword>
    <keyword evidence="13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tetrahydrofolate dehydrogenase/cyclohydrolase catalytic" evidence="23">
        <location>
            <begin position="36"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Tetrahydrofolate dehydrogenase/cyclohydrolase NAD(P)-binding" evidence="24">
        <location>
            <begin position="173"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00012859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00017592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00051435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00051544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00052163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF00763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF02882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="CAR23666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="970" mass="104514" checksum="5FA1B9D049FBDB28" modified="2009-07-28" version="1">MFSRALSRTPLRRFYLPTQQAVTRVPRASPMLYHTLSGTKVAKSIRDEALREIEQIKARVPAFAPMLKIFQVGARPDSNAYVRMKLKASKESGVACDVEKLPEDISEVELLNKIDQVNKDDSVHGVLIQLPLPKHLDETKITNSVLYSKDVDGFHRYNAGELSKKGGEPKFIPCTPNGCIRLLQEAGVDLKGKNAVVLGRSDIVGTPVSSLLKNLDATVTVCHSRTADLPGILSKADVVVAACGKANYVKGEWLKDGAVVIDVGINYIPDASKKSGQRLVGDVDFESAKHKASYITPVPGGVGPMTVAMLVQNVLLAAKRQLSEGNKLPKITPLPIKLLNPVPSDIDISRAQVPKKIDVVAEELGIQPNEVELFGHYKAKISPGVLKRLESNKNGKFVLVAGITPTPLGEGKSTTTMGLVQALTAHLGKPSIANVRQPSMGPTFGVKGGAAGGGYAQVIPMDEFNLHLTGDIHAISAANNLLAAAIDTRMFHESTQKKDATFYNRLVPKKKGKRVFTKSMVKRLEKLGISKTNPDDLTPEEIKRFARLDIDPETITIKRVVDVNDRMLRQITIGQAPTEKGFTRSTGFDITVASELMAILALSRDLKDMRQRVGRIVIGANRDGEPVTAEDVGCAGAITALLKDAIKPNLMQTLEGTPVLVHAGPFANISIGASSVIADRVALKLVGKPKALEESAETGYVVTEAGFDFTMGGERFFNIKCRSSGLTPNAVVLVATVRALKLHGGAPDVKPGQSLPSEYTEENVDLVTKGAANLCKQIANAKQFGVPVLVAINKFETDTEAEIEAIRKAAKEAGAFDAVATNHWAEGGKGSIDLAHAVVKATEQPTNFKFLYDVDQSVEEKLTAIVQKMYGGAKIDILPEAQRKIDMYKQQGFGNLPICIAKTQYSLSHDASLKNVPTGFTFPIRDVRASIGAGYLYALAAEIQTIPGLSTFAGYMNVEVNEDGEIDGLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJ14</accession>
    <name>C5DJ14_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0E16852p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0E16852g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the aldo/keto reductase family. Aldo/keto reductase 2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554212.1">
        <property type="nucleotide sequence ID" value="XM_002554166.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJ14"/>
    <dbReference type="FunCoup" id="C5DJ14">
        <property type="interactions" value="72"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJ14"/>
    <dbReference type="GeneID" id="8292386"/>
    <dbReference type="KEGG" id="lth:KLTH0E16852g"/>
    <dbReference type="eggNOG" id="KOG1575">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_2_0_1"/>
    <dbReference type="InParanoid" id="C5DJ14"/>
    <dbReference type="OMA" id="IALQPHY"/>
    <dbReference type="OrthoDB" id="48988at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047681">
        <property type="term" value="F:aryl-alcohol dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19147">
        <property type="entry name" value="AKR_AKR9A3_9B1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000024">
        <property type="entry name" value="Aryl-alcohol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050523">
        <property type="entry name" value="AKR_Detox_Biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43364:SF2">
        <property type="entry name" value="ARYL-ALCOHOL DEHYDROGENASE AAD10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43364">
        <property type="entry name" value="NADH-SPECIFIC METHYLGLYOXAL REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="3">
        <location>
            <begin position="31"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR23775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="375" mass="42127" checksum="E79C50863CD0FD3A" modified="2009-07-28" version="1">MSELFAPAPEPPTELGRLRILSKNAGIRVSPLVLGAMSIGDAWEETLGSMDKQRSFELLDAFYEAGGNFIDTANNYQNGQSEEWIGEWMSSRKIRDQIVIATKYTSDYKAYQIGKNKSANFSGNHRRSLQLSVRDSLRKLQTDWIDILYVHWWDQMTSIEEVMDSLHVLVQQGKVLYLGVSDTPAWVVSAANTYARAHGKTPFSVYQGRWNVMTRDFEREIIPMARTFGMALVPWDVLGGGRFQTKKSLEEKKNNGENIRTFFGPADQTPNEIKVCEALEKVAKEHNTESITAIALAYVRSKTSRVYPLVGGRKTEHLKQNIEALSIKLSPEQISFLESIVPFDLGFPSSFIGPDPNVTGETGFLTAMSTHLAFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJ17</accession>
    <name>C5DJ17_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0E17006p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0E17006g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 13 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554215.1">
        <property type="nucleotide sequence ID" value="XM_002554169.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJ17"/>
    <dbReference type="FunCoup" id="C5DJ17">
        <property type="interactions" value="953"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJ17"/>
    <dbReference type="GeneID" id="8292393"/>
    <dbReference type="KEGG" id="lth:KLTH0E17006g"/>
    <dbReference type="eggNOG" id="KOG0471">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006462_2_3_1"/>
    <dbReference type="InParanoid" id="C5DJ17"/>
    <dbReference type="OMA" id="WNQPEVH"/>
    <dbReference type="OrthoDB" id="1740265at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004556">
        <property type="term" value="F:alpha-amylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033934">
        <property type="term" value="F:glucan 1,4-alpha-maltotriohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004574">
        <property type="term" value="F:oligo-1,6-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004575">
        <property type="term" value="F:sucrose alpha-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000025">
        <property type="term" value="P:maltose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005987">
        <property type="term" value="P:sucrose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11333">
        <property type="entry name" value="AmyAc_SI_OligoGlu_DGase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000064">
        <property type="entry name" value="Oligo-1,6-glucosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.400.10:FF:000004">
        <property type="entry name" value="Oligo-1,6-glucosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000087">
        <property type="entry name" value="Oligo-1,6-glucosidase IMA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1180:FF:000007">
        <property type="entry name" value="Sucrose isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.400.10">
        <property type="entry name" value="Oligo-1,6-glucosidase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="GH13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045857">
        <property type="entry name" value="O16G_dom_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10357">
        <property type="entry name" value="ALPHA-AMYLASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10357:SF179">
        <property type="entry name" value="NEUTRAL AND BASIC AMINO ACID TRANSPORT PROTEIN RBAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0462">Maltose metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 13 catalytic" evidence="5">
        <location>
            <begin position="21"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00026248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR23778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="584" mass="68021" checksum="97943B2259695403" modified="2009-07-28" version="1">MTISEHPETLSKWWKEATIYQIYPASFKDSNDDGWGDLKGITSKLEYLKKLGVDAIWVCPFYDSPQQDMGYDIANYEKVWPTYGTNEECFELINKTHELGMKFITDLVINHCSSDHEWFKESRSSKSNPKRDWFFWRPPKGYESDGKPIPPNNWKSFFGGSAWSFDEHTNEFYLRLFASRQPDLNWENVDCRKAIYESAVGYWLDHGVDGFRIDTAGLYSKRPNLPDSPIFDVDSDLQHANWGSHNGPRIHEFHRELRKFIDDRVKDGREIMTVGEVAHGNDNPLYSCASRQEMNEVFSFRHVDIGTSPFFRYNTVPFTLKDWKQAIADNFLFINGTDAWATIYIENHDQPRSITRFGDDSPKYRTLSGKLLALLECSLTGTLYVYQGQEIGQVNFKDWPIEKYEDVDVRNNFEIVKNKYGENSKEMRLFYEGIALMSRDHSRTPIPWSPEVPNAGFCGTAAKPWFSLNESYKEGINVAEELENADSVLNFWRKALETRKKHKNVMIYGYDFEFIDLDSEKLFSFTKQYEDKVLFAAFNFSGEHVEFKRPDKNASLSLILGNYNEADTSSTILKPWEGRLYYVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJ71</accession>
    <name>C5DJ71_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Copper transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0F14014g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Membrane</location>
            <topology evidence="1 5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the copper transporter (Ctr) (TC 1.A.56) family. SLC31A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554797.1">
        <property type="nucleotide sequence ID" value="XM_002554751.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJ71"/>
    <dbReference type="FunCoup" id="C5DJ71">
        <property type="interactions" value="163"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJ71"/>
    <dbReference type="GeneID" id="8293023"/>
    <dbReference type="KEGG" id="lth:KLTH0F14014g"/>
    <dbReference type="eggNOG" id="ENOG502QVCV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_819370_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJ71"/>
    <dbReference type="OMA" id="LKRWDIQ"/>
    <dbReference type="OrthoDB" id="73901at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005375">
        <property type="term" value="F:copper ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007274">
        <property type="entry name" value="Cop_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483:SF27">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN CTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 31 COPPER TRANSPORTERS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04145">
        <property type="entry name" value="Ctr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0186">Copper</keyword>
    <keyword evidence="5" id="KW-0187">Copper transport</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002947864" description="Copper transport protein" evidence="7">
        <location>
            <begin position="19"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="79"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="199"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="254"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="326"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="256"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU367022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR24360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="347" mass="38059" checksum="5BEF0DF1235F07E5" modified="2009-07-28" version="1">MVAVKVTTALLAVAWATAAKAMGGDGMVMTTSSSSAMSMATGSSSMEMSDMTMNSYLTPKFYNYPVLFDHLRAKNKGQAFGIFVLIMAAAFAYKLALFVSWCLEVRWFKKWNPSGARRPGTLSSTGPSFDADNASQVTAQNYTQDLEFQSQYLPKMPNLLVHVMSPSSVDLFHDFIRLLLAFCSTMMIYMLMLVAMTFVLTYVFAVILGVSLAEIFFNRWKICLIARWDLQRELKRRRNCKGGEVCLCGQHKPEQKAASGSSSSPTSSLLEKPSTQKPAASNVPCGGKSSCCCGPEEDESLAETNCGCEANSIDDDANNERQARELAKNNEQTGNMDVNLIPAEKFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJC5</accession>
    <name>C5DJC5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0F15334p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0F15334g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554851.1">
        <property type="nucleotide sequence ID" value="XM_002554805.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJC5"/>
    <dbReference type="GeneID" id="8293082"/>
    <dbReference type="KEGG" id="lth:KLTH0F15334g"/>
    <dbReference type="HOGENOM" id="CLU_2441247_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJC5"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="90" mass="10024" checksum="9D0EB5E4B21FC824" modified="2009-07-28" version="1">MPSILLNMGPLPVNISASLSIVIVTAAALPGVVSYYEAPDSIKLAIRRLDMIMDMIMDVLPKRSLFFLLLLNLMLGMEPSSTYSYRSRYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJI4</accession>
    <name>C5DJI4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0F16676g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554910.1">
        <property type="nucleotide sequence ID" value="XM_002554864.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJI4"/>
    <dbReference type="FunCoup" id="C5DJI4">
        <property type="interactions" value="341"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJI4"/>
    <dbReference type="GeneID" id="8293143"/>
    <dbReference type="KEGG" id="lth:KLTH0F16676g"/>
    <dbReference type="eggNOG" id="KOG0201">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009125_2_0_1"/>
    <dbReference type="InParanoid" id="C5DJI4"/>
    <dbReference type="OMA" id="IMEGVYY"/>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000499">
        <property type="entry name" value="Serine/threonine-protein kinase KIC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050629">
        <property type="entry name" value="STE20/SPS1-PAK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012:SF10">
        <property type="entry name" value="FI20177P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="19"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="298"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="411"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="465"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="538"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="742"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="419"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="491"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="538"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="574"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="601"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="752"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR24473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="878" mass="95929" checksum="5BA283E341925725" modified="2009-07-28" version="1">MLSNKTNAGVPKSSVSTLFKRTEVIGRGKFGIVYKGYYIKTNRVCAIKVLNLDSADDEVEDVQKEVQFLSSLKQVPNITHYYGSYLNNTELWVIMEYCAGGSLRTLLRPGKIGEQYIGVIMRELLIALMHIHKDGVIHRDIKAANVLITNDGHIKLCDFGVAAQLSQTKIRRQTMAGTPYWMAPEVIMEGVYYDTKVDIWSLGITAYEIATGNPPYCDVEALRAMQLITKSKPPRLEGRQHSSALKEFIALCLDEDPKERPSAEELLKTKFIRLHKNVSASILKELISRYLLHRDKHSRRESSTSGLEEDESGKVDGSELFGDSNELDVKWDFDSLSSNEYIIENDINIDAIPEETTPNEHFSYAYPEDDLLYQSNQKFYHGTTMGKGHTNVTMNNSTIQASNNNNATYNNTTSNYQSKHTLPNTSKNSETKASKALLQLFEDGDDNSTDVDYARSASALAGKHADDNLKSATPLPDRGQHPDAFKGPIYHSQSTPSLPMLQTNFSQPVILGPPSSTALPTPIEIEIPEELPFAKENMGSSITKPRSSTVSVPNNKPSPLIQRRPTITGGMGSSALSRNDSGLSGSTMKTLQEDKQRLMAPSSNGINNKPGVRSSSPMKLGNTASPKKPLPVTPSTSSAPLAMKPVPGEKSDMLLKPLNGDDSEAGRTRINRDFKRQNPNLKLQMPSPTSLVPNKLLENPSVLSNNPDDANINQFGFNTSTATLPVAMTPVVERNIDLMKRKPSCGASTGNASRSSSVSQEAGSAASSSTAAPSALPQPSNTASIPSAVSSAPANAALIAPAPSLTMERPPRQLQMDMFLDFPDDLPSAEDSSDHHPDRKFLVLRELDSLLKTVEDGLPVMEHALKEAFAKTTTPGEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJI8</accession>
    <name>C5DJI8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Probable cytosolic iron-sulfur protein assembly protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">CIA1</name>
        <name evidence="5" type="ordered locus">KLTH0F16764g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Essential component of the cytosolic iron-sulfur (Fe/S) protein assembly machinery. Required for the maturation of extramitochondrial Fe/S proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Interacts with NAR1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
        <text evidence="3">Preferentially localized to the nucleus.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat CIA1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554914.1">
        <property type="nucleotide sequence ID" value="XM_002554868.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJI8"/>
    <dbReference type="FunCoup" id="C5DJI8">
        <property type="interactions" value="82"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJI8"/>
    <dbReference type="GeneID" id="8293147"/>
    <dbReference type="KEGG" id="lth:KLTH0F16764g"/>
    <dbReference type="eggNOG" id="KOG0645">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_57_8_1"/>
    <dbReference type="InParanoid" id="C5DJI8"/>
    <dbReference type="OMA" id="IREIRWS"/>
    <dbReference type="OrthoDB" id="284782at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000705">
        <property type="entry name" value="Probable cytosolic iron-sulfur protein assembly protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03037">
        <property type="entry name" value="ciao1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028608">
        <property type="entry name" value="CIAO1/Cia1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="53"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="105"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="151"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="195"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR24477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="331" mass="37300" checksum="2AD91A51B11ECB98" modified="2009-07-28" version="1">MSIKLLKSLKLHKDKLWSVDCSKGLMATASSDRKIKLVNLKNLDFQLVEELDDSTHKKSVRSVSFRPHSSILAAGSFDSTISIWGKDEEASIEDGYQETELLAIIEGHENEVKGVAWSHSGYFLASCSRDKSVWIWEADEMGEEYECLSVLQEHSQDVKHVVWHPAMHLLASSSYDDTVRLWKEDADDWECAAVLNGHEGTVWCSDFEKSESSLRLVSCSDDTTVRVWKYEEEDENGEDIWTLESVLPSVHSRAVYAVSWSPDGYIASVGSDGQIVIYTEDESGKWKVIAKQHEAHSVFEINTVQWTRVGDTNLLITGGDDGCANIWFFIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJM7</accession>
    <name>C5DJM7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">KLTH0F17688p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0F17688g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the sorting nexin family.</text>
    </comment>
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        <property type="protein sequence ID" value="CAR24516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_002554907.1"/>
    </dbReference>
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        <property type="interactions" value="811"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJM7"/>
    <dbReference type="GeneID" id="8293188"/>
    <dbReference type="KEGG" id="lth:KLTH0F17688g"/>
    <dbReference type="eggNOG" id="KOG2273">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021752_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJM7"/>
    <dbReference type="OMA" id="EKMAAVW"/>
    <dbReference type="OrthoDB" id="271164at2759"/>
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        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030904">
        <property type="term" value="C:retromer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="P:protein retention in Golgi apparatus"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07627">
        <property type="entry name" value="BAR_Vps5p"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Putative Sorting nexin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000022">
        <property type="entry name" value="Sorting nexin 3 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035803">
        <property type="entry name" value="BAR_Vps5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015404">
        <property type="entry name" value="Vps5_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10555:SF170">
        <property type="entry name" value="FI18122P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10555">
        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09325">
        <property type="entry name" value="Vps5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PX" evidence="12">
        <location>
            <begin position="236"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="15"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="108"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="128"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="165"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR24516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="631" mass="72381" checksum="56B60B10484210FD" modified="2009-07-28" version="1">MDYEDELSAPVWDDQGPREESKTEDELSKTFANLSTDPAVEEHEGSTWNAKNIEHEEEVDEVQNHSKNLISSLAPEEDPLSSLSVSEAVRSPQKGEALFGDSGSAPLEAHEDTSNEAHETSAISKNYTKPKRLFSSARLRRHPLTAEQKKDLFQDPLASSPSTREEDEFVEETLDYEPSPERSKTDILREVDAPLFKVDPRKNQSLTPSIKKVADVGGDETQENPDNKLLDQETPNNYSIEVIDPLKVGDLTSAHVEYTIFTKDKNSNQPEFKVQRRYRDFRWLYRQLQNNHWGKIIPPPPEKQTMGRFENDFIENRRLQMERMLTKIAQDADLRDDTDFILFLQSNNFSQDSKVREHLTGSNASGDSNDISEIHISEIELLGQEDAANVMKNGGLDESHKGFMNISFASAPKYNEPDSFFVEQRQATELLEEQLRQLCKSLEIVDVQRNDLASVTEEFAQTIKALEELEVSKKGSELLANFADVHLRIKESLQRSTLQESLTLGITIDDYLRSLSSIRAVFNQRSKLGYYLVIVENDLAKKQLQLEKNSTKGPSDKVENLSKEVKVLQARHIKIRQKWQEVGDTIRKELQRHDRDKMIDFRNSIEIFLESSIETQKECIELWETFYQNNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJP8</accession>
    <name>C5DJP8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0F18150p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0F18150g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="CAR24537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002554928.1"/>
    </dbReference>
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    <dbReference type="eggNOG" id="ENOG502QRSD">
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        <property type="component" value="Chromosome F"/>
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        <property type="term" value="F:Hsp90 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
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    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
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        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22904:SF523">
        <property type="entry name" value="STRESS-INDUCED-PHOSPHOPROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22904">
        <property type="entry name" value="TPR REPEAT CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="7"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="F-box" evidence="4">
        <location>
            <begin position="204"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR24537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="689" mass="78916" checksum="09DAC437338B7C33" modified="2009-07-28" version="1">MSDEDVVEKALDLGVVYFKNEDYKNANSLFTKAMRLARSYKTEDIESIRVSHGLSKRPFYEIGELVHPRLVKLLDNRAATWEKIGDLKKALKDANRATRFEPYNLKCYLRRGKILQKLRMDGQALENYEEGVKAIRDMTRKYHLDPPKKLYEALEYQKNLVSRRLKGPKTSVASKSERKRTLLPQVMQPTEKKMAVDTRAIPILDILGQCPLEILKKILCNLDSTDLSRCLLVSKVWHSRICLLPSVFNRFDLSSCTYRKIISFQRFINKMYVNAGRQQIDHIKFRSLAASTESKVIPTLLQDLKPLVKTLIIQHQFTDLESITTSLVRNKSLAAGLKEVSLTCEYKKKIGTPLEVTLMALLKKIQKLEMVFPNSSNANRSKVSIEDQNHELFIRNVQNLRSMRIICNHKNTSPHFPFKTIITSEASFRIEKLFISGVTFDTTMGFEWLANFSNLRELWLENNKNAQLEEFLRVVIYKPLFNKLSKLTFREHRITGRPVNLAQYPHLAENCNLLNNFSSIEHLDVMSSSIGAKGLLAVLSAVGPDQLKVLNIGDCPYIHFQRGNSSGFLDMRDLLLKIHHIEELCLHQSVALDDHAIIELAKNVRHIKKIRKLDLSFNMSLTGVSIYQMLRSFKEEFIILDKLIIDGCPSVSEGTVSALKGSGFVRELVCSYEKLAWGTFGINSLKYPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DK20</accession>
    <name>C5DK20_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0F01122p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0F01122g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554258.1">
        <property type="nucleotide sequence ID" value="XM_002554212.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DK20"/>
    <dbReference type="FunCoup" id="C5DK20">
        <property type="interactions" value="128"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DK20"/>
    <dbReference type="GeneID" id="8292449"/>
    <dbReference type="KEGG" id="lth:KLTH0F01122g"/>
    <dbReference type="eggNOG" id="KOG3834">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_742267_0_0_1"/>
    <dbReference type="InParanoid" id="C5DK20"/>
    <dbReference type="OMA" id="SSTQHIW"/>
    <dbReference type="OrthoDB" id="3318at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007583">
        <property type="entry name" value="GRASP55_65"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024958">
        <property type="entry name" value="GRASP_PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12893">
        <property type="entry name" value="GOLGI REASSEMBLY STACKING PROTEIN GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12893:SF0">
        <property type="entry name" value="GRASP65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04495">
        <property type="entry name" value="GRASP55_65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51865">
        <property type="entry name" value="PDZ_GRASP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PDZ GRASP-type" evidence="6">
        <location>
            <begin position="49"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ GRASP-type" evidence="6">
        <location>
            <begin position="165"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="309"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="313"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="339"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR23821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="364" mass="39653" checksum="BE972BB0020AA65A" modified="2009-07-28" version="1">MFRIAKNLVRTFEQSVSDTIGAGTQLDSYFESVPPNLLSPGNNTNELLHGLRVLACDESQVQLQSFFDYIIGIDDQPLPLSQNQHGYLYPDFGSITRLLNAACGSSVKLNLWSGKGGVYRDEYIRVERKKNVEDVPLDGSRDVVFQEFDALGFAVQWTPLTAATYTYHVLELSDPTGPAAQAGFVPQEDYVVGCQEGLLATGGETLLQDILRSHAGQDLVLYVFNAVSDCIRPVTVHVGHDGRLGCNMGYGFLHRIPAPFRQEPASAVEASFADAPMSDQSFVPAGSSNIGIPQPSTDIQPTSTSQFIVPNPTHKRKQKHHKVAAATSIQDYFQEGKDPSPAFSTKSSSTPPPPPPVKNQDTTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DK66</accession>
    <name>C5DK66_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0F02156p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0F02156g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the anion exchanger (TC 2.A.31) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554304.1">
        <property type="nucleotide sequence ID" value="XM_002554258.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DK66"/>
    <dbReference type="FunCoup" id="C5DK66">
        <property type="interactions" value="429"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DK66"/>
    <dbReference type="GeneID" id="8292496"/>
    <dbReference type="KEGG" id="lth:KLTH0F02156g"/>
    <dbReference type="eggNOG" id="KOG1172">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002289_7_2_1"/>
    <dbReference type="InParanoid" id="C5DK66"/>
    <dbReference type="OMA" id="RRAPFYW"/>
    <dbReference type="OrthoDB" id="1735926at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080139">
        <property type="term" value="F:borate efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005452">
        <property type="term" value="F:solute:inorganic anion antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006820">
        <property type="term" value="P:monoatomic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050801">
        <property type="term" value="P:monoatomic ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.570:FF:000003">
        <property type="entry name" value="Anion exchange family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.570">
        <property type="entry name" value="Helical hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011531">
        <property type="entry name" value="HCO3_transpt-like_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003020">
        <property type="entry name" value="HCO3_transpt_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453">
        <property type="entry name" value="ANION EXCHANGE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453:SF82">
        <property type="entry name" value="BORON TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00955">
        <property type="entry name" value="HCO3_cotransp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="158"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="221"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="261"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="317"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="355"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="439"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="482"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="505"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="domain" description="Bicarbonate transporter-like transmembrane" evidence="8">
        <location>
            <begin position="47"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="Bicarbonate transporter-like transmembrane" evidence="8">
        <location>
            <begin position="225"/>
            <end position="536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR23867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="558" mass="62586" checksum="CF4E9DEA0B654982" modified="2009-07-28" version="1">MTLRGGLVSKAPREMFGFWSEDRAKLSQTEEDTDDTQSNLRTKFPKPFAGIAHDLRDRLPVYWSDWSDSWDYRVIPSVIETFFNNLLPAIAFAQDMFDRTDHSYGVNEVLLASTVGGVVFGLLAGQPLCIVGVTGPITIFNYTVYEVIKPLHTNYFGFMFWVCIWSMIFHFLLAIFNAVCLLQYVTTFPCDVFGLFNNVVYMEKGIQILVRQFKKNGKVDLSAGFASTVVALLMTMFGLSSKLITKTPFFSHRIRTFIADYSTALSVVFWSGFIHFGGFLSDIDFQKLPITKSFIPTSHLRDRTTWLAYEAISTRDIFIALPFGLVLTTLFYFDHNVSSLMAQRYQYRLKKASTFHYDFALLGATTGVSGILGIPAPNGLIPQAPLHTESLLVRDKRGEVVRCVEQRFTNTAQGLLTLATMSRPLLVCLGQIPQAVLSGLFFIMGLNNLLHNAILGRIMLLITERDKRDLRNLIYKVPLPKVLIFLSLSLAGFAAEFAISNTIAAIGFPLILLLTVIVSLFFPKWFSPEDLKILDEGVAEEFTLKNLQVSSLTGTSAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKG9</accession>
    <name>C5DKG9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0F04576p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0F04576g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554407.1">
        <property type="nucleotide sequence ID" value="XM_002554361.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKG9"/>
    <dbReference type="FunCoup" id="C5DKG9">
        <property type="interactions" value="26"/>
    </dbReference>
    <dbReference type="GeneID" id="8292602"/>
    <dbReference type="KEGG" id="lth:KLTH0F04576g"/>
    <dbReference type="eggNOG" id="KOG1289">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004495_5_3_1"/>
    <dbReference type="InParanoid" id="C5DKG9"/>
    <dbReference type="OMA" id="LLMAQYT"/>
    <dbReference type="OrthoDB" id="3257095at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649">
        <property type="entry name" value="AMINO-ACID PERMEASE BAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649:SF3">
        <property type="entry name" value="POLYAMINE TRANSPORTER TPO5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="59"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="87"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="132"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="177"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="210"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="251"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="296"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="327"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="401"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="428"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="466"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="494"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="7">
        <location>
            <begin position="71"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR23970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="549" mass="60780" checksum="945FA252B4B8D612" modified="2009-07-28" version="1">MLESVRTRKPFRDGFQLLPQSVWANWGSIIQDADEEEEEVQRFKYEQQLDRSFLSKSSVIGLGFGLMSPVLGMSTTMSIGLLNGGPLTIVIGFLINGIATWLCSLSLGEIVSRFPIELHGGSAMLARQKFKLACSWFTGWLMLLGNWTMSTSITFAGAQLLVSLVTMTNDKLIGENYIVVTTVLAFYLIVTIVGLINLKFAGFIETVNKVCVYWIVYAVIFIDILLLLFHSGKYRSLKYALAHFDNSFSGYSSAALSFIIGFQQSSFTLQGFSMLPALADEVKESERDIPKGMSTAVLTSTVTGVVFLLPIMLILPDTQTIINNPKVMPIALIFTQSTHSIFVSLFLVIMILGNLLFSGIGSVTTSSRAVYSMSRDMALPYSEFWSYVDPSSKHRVPKNSILLSMGISYLLGLLALLSTAAFNAFMGAAVLCLCSATCIPLVLVLISRRRALRGSPIKVRYRLGWFVNIASILWLVFTMVVICIPPQVPVTFSSMNYAFVVYLLFLAAITVLYFRWGRYNFKTPLISEDSRTDDTASASFVLQDANLPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKI7</accession>
    <name>C5DKI7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">KLTH0F05016p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0F05016g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the PIR protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554425.1">
        <property type="nucleotide sequence ID" value="XM_002554379.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKI7"/>
    <dbReference type="FunCoup" id="C5DKI7">
        <property type="interactions" value="67"/>
    </dbReference>
    <dbReference type="GeneID" id="8292622"/>
    <dbReference type="KEGG" id="lth:KLTH0F05016g"/>
    <dbReference type="eggNOG" id="ENOG502QQD8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039662_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKI7"/>
    <dbReference type="OMA" id="IQHQTAK"/>
    <dbReference type="OrthoDB" id="5415592at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005199">
        <property type="term" value="F:structural constituent of cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054508">
        <property type="entry name" value="PIR1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051153">
        <property type="entry name" value="Yeast_CWMannoprotein_PIR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000420">
        <property type="entry name" value="Yeast_PIR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47254">
        <property type="entry name" value="CELL WALL MANNOPROTEIN CIS3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47254:SF1">
        <property type="entry name" value="CELL WALL MANNOPROTEIN CIS3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00399">
        <property type="entry name" value="PIR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22799">
        <property type="entry name" value="PIR1-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00929">
        <property type="entry name" value="PIR_REPEAT_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50256">
        <property type="entry name" value="PIR_REPEAT_2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0134">Cell wall</keyword>
    <keyword evidence="4" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950412" evidence="9">
        <location>
            <begin position="20"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="Cell wall mannoprotein PIR1-like C-terminal" evidence="10">
        <location>
            <begin position="317"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="270"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="273"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="284"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF22799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR23988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="401" mass="39687" checksum="1FB1C8CF8AA60B9A" modified="2009-07-28" version="1">MQYKSTILAAAALATSASAAVTASEPWTTLTPTATFKSGATSYSETFGIAVVPLSSSVSTVSASTSAAHAKRDAISQIGDGQIQATTSTTAAPSKKTSAAAISQIGDGQIQATTSTASAKKSSAAAISQIGDGQIQATTSTAPAKKTSAAAISQIGDGQIQATTSTASAKSSAAAISQIGDGQIQATTSTAAAKKSSAAAISQIGDGQIQATTSTAAAKSSAAAISQIGDGQIQATTTTLKPSSAAAISQISDGQIQASTTTNSTETATAASQVSDGQVQATTSASSSPASSSDASSFIDSVSCKADGTLEMTLEGGILKDGKGRIGSIVANRQFQFDGPPPQAGAIYAAGWTITPEGNLAIGETDTFYQCLSGNFYNLYDESIGAQCSPVHLEVIELVDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKL9</accession>
    <name>C5DKL9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">KLTH0F05786p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0F05786g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554457.1">
        <property type="nucleotide sequence ID" value="XM_002554411.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKL9"/>
    <dbReference type="FunCoup" id="C5DKL9">
        <property type="interactions" value="757"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKL9"/>
    <dbReference type="GeneID" id="8292655"/>
    <dbReference type="KEGG" id="lth:KLTH0F05786g"/>
    <dbReference type="eggNOG" id="KOG0760">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015166_3_1_1"/>
    <dbReference type="InParanoid" id="C5DKL9"/>
    <dbReference type="OMA" id="MYNSQHQ"/>
    <dbReference type="OrthoDB" id="43906at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015093">
        <property type="term" value="F:ferrous iron transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048250">
        <property type="term" value="P:iron import into the mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000054">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45758:SF4">
        <property type="entry name" value="MITOFERRIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45758">
        <property type="entry name" value="MITOFERRIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="16"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="120"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="211"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR24020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="307" mass="33674" checksum="AD156BB88FC38216" modified="2009-07-28" version="1">MEMDEEIDYEALPSSAPLTHQLAAGAFAGIMEHSIMFPIDAIKTRMQAISATVGSSNAAPKLPSNIMQQIARISTTEGSMALWKGVQSVILGAGPAHAVYFATYEMCKGYLIDPQDFQTHQPLKTAASGVAATIAADMLMNPFDTIKQRMQLRTFSNDRMWAVASRIYRNEGLAAFFYSYPTTIAMNIPFAAFNFVIYESSTKLMNPNNSYNPLIHCLCGGLSGATCAAITTPLDCIKTVLQIRGSESVVHPLFRSADTFSKAASAIFKIYGWSGFWRGLKPRIISNMPATAISWTAYECAKHFLFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKP6</accession>
    <name>C5DKP6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0F06446p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0F06446g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554484.1">
        <property type="nucleotide sequence ID" value="XM_002554438.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKP6"/>
    <dbReference type="FunCoup" id="C5DKP6">
        <property type="interactions" value="57"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKP6"/>
    <dbReference type="GeneID" id="8292685"/>
    <dbReference type="KEGG" id="lth:KLTH0F06446g"/>
    <dbReference type="eggNOG" id="ENOG502S83T">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094658_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKP6"/>
    <dbReference type="OrthoDB" id="412109at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043124">
        <property type="term" value="P:negative regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038753">
        <property type="entry name" value="NFKBIL1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15263">
        <property type="entry name" value="I-KAPPA-B-LIKE PROTEIN IKBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15263:SF1">
        <property type="entry name" value="NF-KAPPA-B INHIBITOR-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="32"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="32"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR24047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="274" mass="30715" checksum="61AC35B24C7DD089" modified="2009-07-28" version="1">MAFSQDYLSTSESSDEDEFKYFKPVYVRRRAARPDVPLEEKGKNAACSGSAKAGDCQKNPATQDPRPRPGPYKLKAPGTNAFSGKEELIALERERIPQECSRSQQEVKPQTRITEPTYEYGNPINGYKFVGSRSDYEKFTRAAESSSPSGGADDTNYIDSRIFDRASMHSKRSSHPDDIAVYWARELGATRAPLPGAPLVQEVTIQSIDKASLADFLGRSALSLRLPLREVLKQQRVVWHPDKVFRGSARANPNDAEKVTRIFQVINSLWEENQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKS6</accession>
    <name>C5DKS6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0F07150p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0F07150g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554514.1">
        <property type="nucleotide sequence ID" value="XM_002554468.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKS6"/>
    <dbReference type="FunCoup" id="C5DKS6">
        <property type="interactions" value="447"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKS6"/>
    <dbReference type="GeneID" id="8292717"/>
    <dbReference type="KEGG" id="lth:KLTH0F07150g"/>
    <dbReference type="eggNOG" id="KOG0101">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005965_0_3_1"/>
    <dbReference type="InParanoid" id="C5DKS6"/>
    <dbReference type="OMA" id="DQVLMDH"/>
    <dbReference type="OrthoDB" id="29851at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10232">
        <property type="entry name" value="ASKHA_NBD_HSP70_ScSsz1p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000010">
        <property type="entry name" value="heat shock 70 kDa protein 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639:SF32">
        <property type="entry name" value="HEAT SHOCK PROTEIN PDR13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639">
        <property type="entry name" value="HSC70CB, ISOFORM G-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="457"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="457"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="470"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR24077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="540" mass="58555" checksum="8E9156824A3E98B1" modified="2009-07-28" version="1">MSPIVGIAFGNTTSSIAYVNPKNDVDVIANPDGERTIPSVLSYVGDDEYHGGQALQQLVRNPKNTIINFRDFLGLPFSECDVSRCTAGAPAVEIDGKVGFIVSRGNGKEEKLTVDEVVSRHLNKLKLAAEDYIGQELSQTVISVPTNFTDAQKDALRAAAKKVGLEIIQLINEPSAALLAHTEAYPFEDDVNVVVADFGGVRSDAAVIAVRNGIYTLLATSHDNNLGGENLDAELVEFFAKDFEKKNKSNPRKNAKSMAKLRVNSEITKKTLSNVTTATISIDSLADGFDYHTSINRLRYELVANKVLSKFAQFIEAVITKAELDPLDIDAIVLCGGSSFTPKLSSNLEFLLPESVAVLGPQSKNASNNPNELLAYGAALQAQLLSNYDAEELAQALEPVVINTQHLKKAIGLVDAEGNFVPVLLPETSFPVKKDLTLKNVKGDVLVGVYEGDHHIKETVQEPAAKEESKAEDDDESDWSDEDDEPEVIREKHYTPGIKLMELGIKGAQGLQLSFNVNREGVLRVAARDLKSGNIVKGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKS8</accession>
    <name>C5DKS8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0F07194p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0F07194g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554516.1">
        <property type="nucleotide sequence ID" value="XM_002554470.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKS8"/>
    <dbReference type="FunCoup" id="C5DKS8">
        <property type="interactions" value="873"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKS8"/>
    <dbReference type="GeneID" id="8292719"/>
    <dbReference type="KEGG" id="lth:KLTH0F07194g"/>
    <dbReference type="eggNOG" id="ENOG502QTPX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012589_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKS8"/>
    <dbReference type="OMA" id="FALFEFW"/>
    <dbReference type="OrthoDB" id="4067996at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="220"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="481"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="606"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="273"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="308"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="331"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="398"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="433"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="529"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="552"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="631"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="664"/>
            <end position="673"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="929" mass="103574" checksum="E5C223A1A32CF9DD" modified="2009-07-28" version="1">MELASPENSFEPLSTLKNEKHTNQADAGISTNNSSGAQLDNYKHSGGQETESETGIRPAAGAVKDAHEGHGPEATELRPEQHPGETVASSEHVTVEKQPVLATYSESKNDNGSAAQRGPSDLHSIQSQVQLHSDAIAQLGKKVQQVETSVCSNDEDLNQVLGNLTSATKSLAHNQSVLENKLEDLLRNQVNTDSSVNNLLEKLDQVSQFLNKAARNNTLLASAPPQQIHRGPGRPPKTRNGLASAAAALSMTSKDTLPDSSVGIPRSKRIFQDSDSATSNEESGTTAVNRTATRSMPSSAPDQELPPVKKRGPGRPPKRRNHWAEKKREALAQSASQAGAGYQGDEQPQPLGKDNGESNPGGDGETEFSTFEEPKVEEESLPATPSDDGMQAGPSSTRADELYGEQKTHRGRGRPSTKSSDKPPPLTQAQIKRQQELERRRDAREQMLVSMKYSDRNKAKLFMESNKELLRAMREEERKKRMTAFSYDSAGPASNAMVGNTPSPKPPSSSESRQHPAFLPVSQDSSAQSEHDVVMREEFRGNSSENFLESRPSSHEDGREGTPHSEATNEPTEEPMLASIAVSSSFNTPDPGVVPRKVGILSMLNEDVDDSGSSNKRQLPDIYKGGFDPEFPNKREKLDDEEKRDFRSMIPMLPEKKDNRGRPANSSSSGDNSTALLLASPVELICRNGYFYRKSDREVPITTGTYLEFKFKSKEDDLIRLTMSQEDYAELTKQDRINAYFLKPEIKEETEFASAILSKIVLTEKYVNSLEYFLMEFRWENRLVGLGLKLRESKRTWQRRKALFALFEFWRDKSREKRGFPEFTMMHAVKEMENYRIFINRSVSWFYNHITLLKMVLFELCDNIDTQWREWMFPRGQPLPVLGGSITEDNFNEAIDEVLALDFLEDGTENRDIRASSMPNLPSDSATEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKU0</accession>
    <name>C5DKU0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0F07480p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0F07480g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554528.1">
        <property type="nucleotide sequence ID" value="XM_002554482.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DKU0">
        <property type="interactions" value="201"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKU0"/>
    <dbReference type="GeneID" id="8292732"/>
    <dbReference type="KEGG" id="lth:KLTH0F07480g"/>
    <dbReference type="eggNOG" id="ENOG502REBM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004727_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKU0"/>
    <dbReference type="OMA" id="MLVKHNT"/>
    <dbReference type="OrthoDB" id="2123378at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000142">
        <property type="term" value="C:cellular bud neck contractile ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007120">
        <property type="term" value="P:axial cellular bud site selection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097271">
        <property type="term" value="P:protein localization to bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13278">
        <property type="entry name" value="PH_Bud4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052007">
        <property type="entry name" value="Bud4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36100">
        <property type="entry name" value="BUD SITE SELECTION PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36100:SF1">
        <property type="entry name" value="BUD SITE SELECTION PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="4">
        <location>
            <begin position="1281"/>
            <end position="1392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="195"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="311"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="468"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="518"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="591"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="684"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="775"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="204"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="317"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="557"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="597"/>
            <end position="627"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR24091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1412" mass="157244" checksum="FDCF3F298E5F88E0" modified="2009-07-28" version="1">MVSALGVPAPLFTYCLFRLRIALDVNKRLSHGRCLERRYLNLALRFPHANSYFLSSQKGLALSIARLTSPRLHKMFLDQSATAQSASATVDSLLSEIEHEIPIHAKRQIPLQEIGDDTMEEIVRYNTRSNSSAAAVDPDTEPLHSLLENGNRKSVMFSDDFELHEYSAVDDAAAGSAPASPVVWDLAPLPQVRAGAGSARKTSLHLERDHSDTETDSEDQSLNETFQLGHAELRANTVSNMDERLAHVYDNHHSSLNVPRLKEMVNDIDQQANFQTTSEVHTQPLKITFESTRPLIDSSDDPQMLEFELQSRDSDDQAFTSAQHSPSKIPTTNTIRINRLSTAEPLTASGRLSSGSSVDESETDLETTMRQDKFQLLRTSAPPDLSKPLNAHSYHVSSRSLLSHNADNNSRAFSMATTADEFQSAKESISCDMSADESQSNYSVEDLQISTGMVREDDTLGSITQSMQSDIKHSHSLGASGADGYPPSLPPLNTSYVDRISKSGSTSTVKITSLQNLKGHISESDTERYDDEEAEQGKFSSSPGSDNENDEPIPFTRTSCHSSINRSSSAHSLSNTSAKDIELTNNSAEPLQKAEQTEPQSARDNSSEAADTASMHSGASASGIPNQLQDTRIVSNNFSKLFDEDAIFGDLEETSNDSIDITSSVKPSDYLSIWRSQEVNTKSSPTISANSQFSQQSSGTAGSSISMHSNFKLKPRIISRSRICYPPPKSENAFKLRDICSVPQASGIGILDPLRLKPKVSREISVDLKLASVTQSPRELSHDALQESTCVSRDADANTDNSEEPASRETNILTVEKNQPEEPMTPVKEKRVASVNLSLPDISGDNFDVSKELGDVLGTLNHDEMSLKQTSAGKNSDSLVYDIWDENNYQNGVELELDDQDEKQTKEIFNGGVLTKLLSQDSKAVDEDGEQRPKGGLGLLRNTDSDVAVCRADSIKGLDVIRSASSDSVQHSFEHFKQPHTPSPIKASHIDSPFKIVRARKLINNKVISPDSIAKTELSTEAASSEHDVSISGSNKTVVNEEREKESLFTADPALLEDLGKFYIFVKSIQKLRLEDIERHSPEVSLELDNGTHIVQTPWQPLKKEGIQVKQEFELIMKNEELGTTSQLVITVKCRYKRPQAELVEVIERIPIKKKVPFGKTKYTTNKKYVQKKLDFDAWDLKFAQDGSFARCTVPLRSDVLKQTSYTQKSYRFSLYNEWERQKNPSQQQAKLCELPRKPAYEVGEVTLEMCFLPRTSKHEKFPKTLKLAQDVVDKYNEQQRINFQGYLWQEGGDIDCLLQKRYFVLKGTQLVAHHEITKKPQALINLLKVVSVVGEGKITEDNIKKVRNFTDIVLFSECFKLIFENGEVINLDAESAELKSKWTELLTRVVELNKFHQPWVKHFSNNRAMDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>C5DL00</accession>
    <name>C5DL00_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sister chromatid cohesion protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0F08844g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the SCC2/Nipped-B family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554588.1">
        <property type="nucleotide sequence ID" value="XM_002554542.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DL00">
        <property type="interactions" value="288"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DL00"/>
    <dbReference type="GeneID" id="8292794"/>
    <dbReference type="KEGG" id="lth:KLTH0F08844g"/>
    <dbReference type="eggNOG" id="KOG1020">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_259053_0_0_1"/>
    <dbReference type="InParanoid" id="C5DL00"/>
    <dbReference type="OMA" id="FNSRHVL"/>
    <dbReference type="OrthoDB" id="418242at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090694">
        <property type="term" value="C:Scc2-Scc4 cohesin loading complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061775">
        <property type="term" value="F:cohesin loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140588">
        <property type="term" value="P:chromatin looping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034087">
        <property type="term" value="P:establishment of mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071169">
        <property type="term" value="P:establishment of protein localization to chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990414">
        <property type="term" value="P:replication-born double-strand break repair via sister chromatid exchange"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026003">
        <property type="entry name" value="Cohesin_HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024986">
        <property type="entry name" value="Nipped-B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033031">
        <property type="entry name" value="Scc2/Nipped-B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21704:SF18">
        <property type="entry name" value="NIPPED-B-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21704">
        <property type="entry name" value="NIPPED-B-LIKE PROTEIN DELANGIN SCC2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12765">
        <property type="entry name" value="Cohesin_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12830">
        <property type="entry name" value="Nipped-B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Sister chromatid cohesion C-terminal" evidence="7">
        <location>
            <begin position="1188"/>
            <end position="1362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR24151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1464" mass="165467" checksum="CED1AB192977DCB2" modified="2009-07-28" version="1">MASFPGDGTNIPKRLVECLEFQPLNHLIPKDGLVKLLRSSLELYPALSEDAFSETLQDHSKAEKKVFTPLGKNGKLNIRFRELNSVQPSPTGPVLNLFDGLSEKAKQFLNTECLSSLKRLHSDDEMSDVDEETSSLKRSETTGSEYGDVELRLTPKKLKTGFEEFSLNQSALGLQHINDLKDLMAYIGREDSEADTANQLFWTKLGPNYVLSETSAERIFIILKNVSSMSDVSKILDVKTLGRILNVCSDSVDVILREGDARSFNRIAFKCSFSIFMIFLLDVSEKRLYLERYIISAISFLSRINDKLAESETSLKASNEDCIQLKTLLSLLSSYIQKKPMQEEGLITRIVYLLADLLTFSPTTLAGTSGSATWLESLKVESSEGLISIFQSLPSQQLFIIDELLFRLEFLPNNRVLKKLKRVDHSFYATHLFFTLVSMLQTINSAKLRSQASQWDEESVENFLSLFKEQQELLSTLVEHINNSVLNKCFSGNASKKFILDNYVQDLVSMVARPNWSVAELLLASLTKKLLFAFSPSQQTSSTKESSILQAISHIGSGIQNIRLQSPSDELTIFRIHNNPELLDRAMDAFRNCVISMVPNGNWTAQTQSFWDRQIAFLVALGDFDSSDSDWKERTTMEVTSLLQKPLILQADNNFDNEVKPHENAQLSYFATLHLFELINLFNPYVKLILSLLDKQKVKLRSGAIKCLTPLISKDPSLLADDSIKLVIEQRLKDSSPSVKTAILDLLSLSQYTEFYHLININFNDDSASIRKHILKLNIEIYDNCENLSTKSYVAARILKRTEDEEDSIVERAQSALLERWFLILSGLETSPEEQNSKAKEITHVISAIVGLEESFAELFSVFLNDFVLNKAFHEPNVLTNIMESAHVITGKVVDDAINVHYAAESDDNLETHKDAELNTFRLLSIISSSDHAFVTKDHIATLYPYLLTPQRSNLQYYILKVLKNSFPKLFYFKTKFLVDLETTVLGKLPRMTPLELEEALPLCWSIVKHRGDESRVSKACSSCLAQLSPYINLASKNPSAVTADGKIQKLLYLAAGFARSCPFENTKENFPNLKTREPIFEYVTKCLLVFTKIEIPFVIRKTALRCLLKVAASFPKLFNSRHVLTVIDREFSSGLLDLNLVMIRCLHEFFELEEHKSFKLARPTVRTPSRINRSRSLGFEKPNDTICSAISSRYLKRILKIAFLEDARGSCIALDFIFLTLSLGYANPSYSTATIIALTVSPYAETKKLAMTALESITMKTSSMLFSNLSSGVKMGLEHSKWLQENGLPCNSMFLRGMQELITSCSPANTRFLKSVTKTLVSYLNEDPRSFPKDQILLLCNNFSTLTYENMLEVCTLIRLFDVKIEELGESIEDEVQDSLDEVDNEDVSNLIISHACLFELRAFLCEKYGVSDEAVATVDTAEEDELKSQPAEPKKNKLVFGFSPSEFECKKWYNHYISVDNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>C5DL68</accession>
    <name>C5DL68_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">KLTH0F10428p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0F10428g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the CYC8/SSN6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554656.1">
        <property type="nucleotide sequence ID" value="XM_002554610.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DL68"/>
    <dbReference type="FunCoup" id="C5DL68">
        <property type="interactions" value="391"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DL68"/>
    <dbReference type="GeneID" id="8292866"/>
    <dbReference type="KEGG" id="lth:KLTH0F10428g"/>
    <dbReference type="eggNOG" id="KOG1124">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006762_3_0_1"/>
    <dbReference type="InParanoid" id="C5DL68"/>
    <dbReference type="OMA" id="CLYGMQN"/>
    <dbReference type="OrthoDB" id="418911at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017053">
        <property type="term" value="C:transcription repressor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001929">
        <property type="entry name" value="Cyc8p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000078">
        <property type="entry name" value="Transcriptional corepressor Cyc8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000163">
        <property type="entry name" value="Transcriptional corepressor Cyc8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051630">
        <property type="entry name" value="Corepressor-Demethylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14017:SF1">
        <property type="entry name" value="LD02225P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14017">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13174">
        <property type="entry name" value="TPR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="4 9" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="62"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="96"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="130"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="166"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="203"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="312"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="346"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="439"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="493"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="456"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="495"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="562"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="595"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="631"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="651"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="693"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="726"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="744"/>
            <end position="758"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR24219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="795" mass="87299" checksum="A21C0181A8D7701A" modified="2009-07-28" version="1">MNTLPSQGALATSAVGQLSPRVQQVSPAQLVANIAVQQQQQPVVQVQAQAQAPADPLTQSTAETWLSIASLAETLGDADRAAMAYDATLQYNPSSTKALTSLAHLYRSRDMFQRAAELYQRALSINPELGDIWATLGHCYLMLDDLQRAYAAYQQALYHLSNPNVPKLWHGIGILYDRYGSLDYAEEAFAKVLELDPQFEKANEIYFRLGIIYKHQGKWNQALECFRYILTQPPAPLQEWDVWFQLGSVLESMSEWQGAREAYEHVLLQNERHAKVLQQLGCLYGMQNVQFYDTQTALNLLLKSLEVDSTDATTWYHLGRIHMVRNDYTAAYDAFQHAVNRDSRNPTFWCSIGVLYYQISQYRDALDAYTRAIRLNPYISEVWYDLGTLYETCNNQLTDALDAYKQAARLDTNNVHIRERLEALTEQLANQSNGNVMAHQPKAATASNPPPVMLQPTLQSTDDGNPLNKSSVYVQPQMQAQMQNALPGHMQPHVQAQLQSQALSQQQLAPQPQLQMQGHQQPQLQQFRPLPQGVGPPVVGNSVPFNESAHSTGSSGGQTHALPQIINQPQQLNPGSNKPPQKRPMEGGMDTLVNAAVSSAATPPSDASPAPFSKNTGAAVEGTEPKRRKPSTSATKSLLAQSPQQASGTKSAAASPASQESQAPAVNPPAAKAIGESPHEEPSVADLAAVATPQEVQPQAEVPVQEKTKNSEPQPSQADTPDKSETAASTAPENQTVIKASPDAIEKLREEAELRKEEEQEALANEPSAAQDGNAKPPVNERAVRDVEEDENYDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DL98</accession>
    <name>C5DL98_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Cyclin-dependent kinases regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0F11220g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Binds to the catalytic subunit of the cyclin dependent kinases and is essential for their biological function.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the CKS family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554686.1">
        <property type="nucleotide sequence ID" value="XM_002554640.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DL98"/>
    <dbReference type="FunCoup" id="C5DL98">
        <property type="interactions" value="658"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DL98"/>
    <dbReference type="GeneID" id="8292899"/>
    <dbReference type="KEGG" id="lth:KLTH0F11220g"/>
    <dbReference type="eggNOG" id="KOG3484">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140546_0_0_1"/>
    <dbReference type="InParanoid" id="C5DL98"/>
    <dbReference type="OMA" id="RPINYGQ"/>
    <dbReference type="OrthoDB" id="440676at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030295">
        <property type="term" value="F:protein kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.170.10:FF:000002">
        <property type="entry name" value="Cyclin-dependent kinases regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.170.10">
        <property type="entry name" value="Cyclin-dependent kinase, regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000789">
        <property type="entry name" value="Cyclin-dep_kinase_reg-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036858">
        <property type="entry name" value="Cyclin-dep_kinase_reg-sub_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23415">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASES REGULATORY SUBUNIT/60S RIBOSOME SUBUNIT BIOGENESIS PROTEIN NIP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01111">
        <property type="entry name" value="CKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00296">
        <property type="entry name" value="CYCLINKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01084">
        <property type="entry name" value="CKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55637">
        <property type="entry name" value="Cell cycle regulatory proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00945">
        <property type="entry name" value="CKS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 5" id="KW-0132">Cell division</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00068939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU311113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR24249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="110" mass="13161" checksum="7837AAC2A25B5825" modified="2009-07-28" version="1">MYQHGHAFQGRKLTDQERARVLEFQDTIHYSPRYSDDTHEYRHVMLPKAMLKVIPSDYFNSETGTLRILTEDEWRGLGVTQSLGWEHYECHAPEPHILLFKRVLDYKVER</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLB2</accession>
    <name>C5DLB2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Trehalose-6-phosphate synthase</fullName>
            <ecNumber evidence="6">2.4.1.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">UDP-glucose-glucosephosphate glucosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0F11550g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>D-glucose 6-phosphate + UDP-alpha-D-glucose = alpha,alpha-trehalose 6-phosphate + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18889"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58429"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="EC" id="2.4.1.15"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Carbohydrate biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the glycosyltransferase 20 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.15" evidence="6"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554700.1">
        <property type="nucleotide sequence ID" value="XM_002554654.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLB2"/>
    <dbReference type="FunCoup" id="C5DLB2">
        <property type="interactions" value="672"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLB2"/>
    <dbReference type="GeneID" id="8292913"/>
    <dbReference type="KEGG" id="lth:KLTH0F11550g"/>
    <dbReference type="eggNOG" id="KOG1050">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002351_7_2_1"/>
    <dbReference type="InParanoid" id="C5DLB2"/>
    <dbReference type="OMA" id="NRTIWPL"/>
    <dbReference type="OrthoDB" id="755951at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005946">
        <property type="term" value="C:alpha,alpha-trehalose-phosphate synthase complex (UDP-forming)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003825">
        <property type="term" value="F:alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004805">
        <property type="term" value="F:trehalose-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03788">
        <property type="entry name" value="GT20_TPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000007">
        <property type="entry name" value="Trehalose-6-phosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000035">
        <property type="entry name" value="Trehalose-6-phosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001830">
        <property type="entry name" value="Glyco_trans_20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012766">
        <property type="entry name" value="Trehalose_OtsA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02400">
        <property type="entry name" value="trehalose_OtsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788:SF106">
        <property type="entry name" value="BCDNA.GH08860"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788">
        <property type="entry name" value="TREHALOSE-6-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00982">
        <property type="entry name" value="Glyco_transf_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR24263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="507" mass="57493" checksum="8CDA80F1AA8F798E" modified="2009-07-28" version="1">MAETVVNSLKNALSAKDDKATGIENHPGNVVVVSNRLPVTITKNNSTGKYEYSMSSGGLVTALQGLKKSTTFQWYGWPGLEIPDEEKEQVKKDLLEKFNAIPIFLSDEVADLHYNGFSNSILWPLFHYHPGEINFDENAWLAYNEANMAFAQEIEKNLSDNDIVWVHDYHLMLLPEMLRSGIKQKHLKNIKLGWFLHTPFPSSEIYRILPVRQEILKGVLSCDLVGFHTYDYARHFLSSVQRVLNVNTLPNGVEFEGRFVNVGAFPIGIDVDNFTEGLKQESVIERIKQLKKTFKDVKIIVGVDRLDYIKGVPQKLHAMEVFLKEHPEWIGKVVLVQVAVPSRGDVEEYQYLRSVVNELVGRINGQFGTVEFVPIHFMHKSVPFQELISLYAASDVCLVSSTRDGMNLVCYEYIACQQEKKGSLILSEFTGAAQSLNGALIVNPWNTDELAESIYESLTIPEEKKDANWEKLHKYISKYTSAFWGENFVHELYKIAPNGGSHEAETK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLB6</accession>
    <name>C5DLB6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 8">Spliceosomal protein DIB1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0F11638g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 8">Essential role in pre-mRNA splicing. Also essential for entry into mitosis (G2/M progression) as well as for chromosome segregation during mitosis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the DIM1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554704.1">
        <property type="nucleotide sequence ID" value="XM_002554658.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLB6"/>
    <dbReference type="FunCoup" id="C5DLB6">
        <property type="interactions" value="990"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLB6"/>
    <dbReference type="GeneID" id="8292917"/>
    <dbReference type="KEGG" id="lth:KLTH0F11638g"/>
    <dbReference type="eggNOG" id="KOG3414">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117348_0_0_1"/>
    <dbReference type="InParanoid" id="C5DLB6"/>
    <dbReference type="OMA" id="GMYELYD"/>
    <dbReference type="OrthoDB" id="147752at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02954">
        <property type="entry name" value="DIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000004">
        <property type="entry name" value="Spliceosomal protein DIB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004123">
        <property type="entry name" value="Dim1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12052:SF5">
        <property type="entry name" value="THIOREDOXIN-LIKE PROTEIN 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12052">
        <property type="entry name" value="THIOREDOXIN-LIKE PROTEN 4A, 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02966">
        <property type="entry name" value="DIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017199">
        <property type="entry name" value="mRNA_splic_U5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01410">
        <property type="entry name" value="DIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4 8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00055473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00067627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR017199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR24267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="142" mass="16837" checksum="0EC7BEFE7C5E046E" modified="2009-07-28" version="1">MGSVFLPHLHSGWHVDQAIVTEQERLVVVRFGRDWDKDCMLMDEVLYSIAEKVRRFAVIYLCDIDEVPDFNDMYELYDPMTVMFFYRNKHMMCDFGTGNNNKMNFVVEDKQEMIDILETIFRGARKNKGLVVSPYDYNSKRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLC3</accession>
    <name>C5DLC3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0F11814p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0F11814g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554711.1">
        <property type="nucleotide sequence ID" value="XM_002554665.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLC3"/>
    <dbReference type="FunCoup" id="C5DLC3">
        <property type="interactions" value="107"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLC3"/>
    <dbReference type="GeneID" id="8292925"/>
    <dbReference type="KEGG" id="lth:KLTH0F11814g"/>
    <dbReference type="eggNOG" id="ENOG502RY3X">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062466_0_0_1"/>
    <dbReference type="InParanoid" id="C5DLC3"/>
    <dbReference type="OMA" id="SCPKYYC"/>
    <dbReference type="OrthoDB" id="2402916at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051694">
        <property type="entry name" value="Immunoregulatory_rcpt-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018571">
        <property type="entry name" value="Membrane_anchor_Opy2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15549">
        <property type="entry name" value="PAIRED IMMUNOGLOBULIN-LIKE TYPE 2 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09463">
        <property type="entry name" value="Opy2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="133"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="Membrane anchor Opy2 N-terminal" evidence="7">
        <location>
            <begin position="66"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="184"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="335"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="386"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="432"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF09463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR24274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="449" mass="47236" checksum="08DB96F39CE08A6E" modified="2009-07-28" version="1">MEAVSSRVGPPTQARRPERTVRVLEEWPEWENMKIYRRDESSSAAAAASSTVSSSSAKATSAGSGCVSCPQGSPSCPVCADDEYCAVSLLSCTQCPQTYCTKKKGSVSESSAAASASNSSGASSGSKNASARVGGIVGGVVGGVALIAALLLLWLYFKYWRKSRSRNKDVVIAREEYAGEYDDDKEKDGFREAGAGAGAGGAEGSSLRDSRALYQARNRSSAATQMTKASNILPIAYIPGVTAGSRSQHKLPPLPRHLLRNGDTRSHITLGSSILGGGDDDRESLIDLPLEDSREGTSAASPAAEGVYSGANAEHVTSQDALTTAIRARPKLVQISEEDEEHGNSSSGEAEDTHSGKHFGVTTVLLRSSGDTSRQDDTAEDVPQSDNDDRDSSDDNDDGSFILDVGMQGSLRNATQGEYASVTAARESTLGEDMHREGSGSPFEDKFEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLU1</accession>
    <name>C5DLU1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">V-type proton ATPase subunit D</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">V-type proton ATPase subunit d</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10 11">Vacuolar proton pump subunit D</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0G03498g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Vacuole membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
            <orientation evidence="9">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the V-ATPase D subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555189.1">
        <property type="nucleotide sequence ID" value="XM_002555143.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLU1"/>
    <dbReference type="FunCoup" id="C5DLU1">
        <property type="interactions" value="946"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLU1"/>
    <dbReference type="GeneID" id="8293453"/>
    <dbReference type="KEGG" id="lth:KLTH0G03498g"/>
    <dbReference type="eggNOG" id="KOG1647">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069688_0_1_1"/>
    <dbReference type="InParanoid" id="C5DLU1"/>
    <dbReference type="OMA" id="REEFFRM"/>
    <dbReference type="OrthoDB" id="7676488at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016471">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.3240:FF:000002">
        <property type="entry name" value="Vacuolar atp synthase subunit d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00271">
        <property type="entry name" value="ATP_synth_D_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002699">
        <property type="entry name" value="V_ATPase_D"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00309">
        <property type="entry name" value="V_ATPase_subD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11671">
        <property type="entry name" value="V-TYPE ATP SYNTHASE SUBUNIT D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01813">
        <property type="entry name" value="ATP-synt_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="5" id="KW-0926">Vacuole</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="217"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="240"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00046254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR24752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="276" mass="31211" checksum="2B405AC7CBE904EF" modified="2009-07-28" version="1">MSANREQVFPTRMTLGLMKAKLKGANQGYSLLKRKSEALTKRFRDITRRIDDAKQKMGRVMQTAAFSLAEVSYATGENIGYQVQENVSTARFRVKARQENVSGVYLPQFESYIDEEINDFKLTGLGRGGQQVQRAKEIYSKAVETLVELASLQTAFVILDEVIKVTNRRVNAIEHVIIPRTENTIAYINSELDELDREEFYRLKKVQEKKQQQTAELDALMKKAKAQRAQEDEASTTSKDASKKPDPKSSAKEPTDAPPSQEAPDLLAEQEDDVIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLX2</accession>
    <name>C5DLX2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Leucine carboxyl methyltransferase 1</fullName>
            <ecNumber evidence="3 8">2.1.1.233</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0G04224g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Methylates the carboxyl group of the C-terminal leucine residue of protein phosphatase 2A catalytic subunits to form alpha-leucine ester residues.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>[phosphatase 2A protein]-C-terminal L-leucine + S-adenosyl-L-methionine = [phosphatase 2A protein]-C-terminal L-leucine methyl ester + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:48544"/>
            <dbReference type="Rhea" id="RHEA-COMP:12134"/>
            <dbReference type="Rhea" id="RHEA-COMP:12135"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90516"/>
            <dbReference type="ChEBI" id="CHEBI:90517"/>
            <dbReference type="EC" id="2.1.1.233"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the methyltransferase superfamily. LCMT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.233" evidence="3 8"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555220.1">
        <property type="nucleotide sequence ID" value="XM_002555174.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLX2"/>
    <dbReference type="FunCoup" id="C5DLX2">
        <property type="interactions" value="579"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLX2"/>
    <dbReference type="GeneID" id="8293486"/>
    <dbReference type="KEGG" id="lth:KLTH0G04224g"/>
    <dbReference type="eggNOG" id="KOG2918">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031312_1_0_1"/>
    <dbReference type="InParanoid" id="C5DLX2"/>
    <dbReference type="OMA" id="ESRHLEM"/>
    <dbReference type="OrthoDB" id="203237at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018423">
        <property type="term" value="F:protein C-terminal leucine carboxyl O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016651">
        <property type="entry name" value="LCMT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007213">
        <property type="entry name" value="Ppm1/Ppm2/Tcmp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13600">
        <property type="entry name" value="LEUCINE CARBOXYL METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13600:SF21">
        <property type="entry name" value="LEUCINE CARBOXYL METHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04072">
        <property type="entry name" value="LCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016305">
        <property type="entry name" value="LCM_mtfrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 8" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="176"/>
            <end position="177"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR016305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR016305-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR24783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="331" mass="37643" checksum="3EF7B8949E0104C2" modified="2009-07-28" version="1">MDRAVQETDYDAFSCKVSAIAKHYLPRQVSGKEKEEGEFVQLHMAYAQGLKSVSRRAYSKVDRAVQSAQPVMNYGTYLRSVAIDEAIACFLKEHVKSGAEAPVQVVNLGCGSDLRMTQMLSQVPHLKWLDLDFKESVALKSRILENNPLFQRELGPSESVETAGYLTNRYCLTSCDLNDIAQVAEILKKFTTQDCPTLVLSECVLCYMKSAESKALINCIESFYKMGTWISYDPIGGDDSADKFGKIMQTNLRESRHLEMPTLMVYNSPEKYAQRFNTAPHSTSIVTMWQYYVQSVPDEEKMRLKTLQFLDEIEELEIILSHYILAEASWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLY2</accession>
    <name>C5DLY2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">adenine phosphoribosyltransferase</fullName>
            <ecNumber evidence="7">2.4.2.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0G04444g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>AMP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + adenine</text>
            <dbReference type="Rhea" id="RHEA:16609"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.4.2.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.7" evidence="7"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555230.1">
        <property type="nucleotide sequence ID" value="XM_002555184.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLY2"/>
    <dbReference type="FunCoup" id="C5DLY2">
        <property type="interactions" value="607"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLY2"/>
    <dbReference type="GeneID" id="8293496"/>
    <dbReference type="KEGG" id="lth:KLTH0G04444g"/>
    <dbReference type="eggNOG" id="KOG1712">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063339_1_0_1"/>
    <dbReference type="InParanoid" id="C5DLY2"/>
    <dbReference type="OMA" id="ITHFVYH"/>
    <dbReference type="OrthoDB" id="363185at2759"/>
    <dbReference type="UniPathway" id="UPA00588">
        <property type="reaction ID" value="UER00646"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002055">
        <property type="term" value="F:adenine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003999">
        <property type="term" value="F:adenine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006168">
        <property type="term" value="P:adenine salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044209">
        <property type="term" value="P:AMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000004">
        <property type="entry name" value="Adenine phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00004">
        <property type="entry name" value="Aden_phosphoribosyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005764">
        <property type="entry name" value="Ade_phspho_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050054">
        <property type="entry name" value="UPRTase/APRTase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01090">
        <property type="entry name" value="apt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002636">
        <property type="entry name" value="PRK02304.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32315">
        <property type="entry name" value="ADENINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32315:SF3">
        <property type="entry name" value="ADENINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="12">
        <location>
            <begin position="36"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00011893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR24793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="186" mass="20425" checksum="C3D179F2F3F681AC" modified="2009-07-28" version="1">MPNSSVAQELRSALHQYPNFPSKGILFEDFLPIFRSPQLFGKLVKAFKTHFEEKFAGVKIDYIVALEARGFLFGPAVALEIGAGFVPVRKQGKLPGECLHATYVKEYGSDVFDIQVESIPEGSNVVIMDDIIATGGSALAAGELIEKLGANLLEYAFFMELDFLKGREKLQAPVFTLLTGQEHGLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLY5</accession>
    <name>C5DLY5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0G04510p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0G04510g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the putative lipase ROG1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555233.1">
        <property type="nucleotide sequence ID" value="XM_002555187.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLY5"/>
    <dbReference type="FunCoup" id="C5DLY5">
        <property type="interactions" value="20"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLY5"/>
    <dbReference type="GeneID" id="8293499"/>
    <dbReference type="KEGG" id="lth:KLTH0G04510g"/>
    <dbReference type="eggNOG" id="KOG4372">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007367_1_0_1"/>
    <dbReference type="InParanoid" id="C5DLY5"/>
    <dbReference type="OMA" id="FSNIGCD"/>
    <dbReference type="OrthoDB" id="5368485at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007751">
        <property type="entry name" value="DUF676_lipase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044294">
        <property type="entry name" value="Lipase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016445">
        <property type="entry name" value="Rog1_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482">
        <property type="entry name" value="LIPASE ROG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482:SF20">
        <property type="entry name" value="LIPASE YDR444W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05057">
        <property type="entry name" value="DUF676"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005412">
        <property type="entry name" value="UCP005412_abhydr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF676" evidence="4">
        <location>
            <begin position="180"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="418"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="466"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="418"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR24796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="628" mass="71178" checksum="5FFE4AB7A3844BC3" modified="2009-07-28" version="1">MTVSSEEGLLLVDDRLSLKLGQLIRYKITVDKSLLEPHLTSISELYLAFKNTESALLKPVYLTGPYSFYVDIQPCNYNEWEGYDEEVAFCDDVKPNDEYRAVLKLNNNSRFENSEKFVWQINVVSQLSVTNLPSLRFRLCLTTKPTPEMPKGPVEFPKGVVCEKWDTHRLWNLPPRSPDKPVHLVILTHGIFSNAGCDMVYLKDRIEATASTVGNGSETSNLIVRGFKGNQGRSSKGVKSNGIALAKYIIETIDNLKIRYDLRYISFVGHSLGGLTQSMAIRYICIERPDIFDSSNGLEPLNFITLASPYLGVAGEVPPFVTAILDIGALGQTGVDLNLNRTFFLRKEGIVRKDQHLGSYKRKPLLEIIPSEPLKSLMHRFKNRTTYANILHDGIVPLRTAALLYLDWKALSDVRGIRKENGKGDKGTPDSTSKNDVTGKIPEESMDKKSALKYLLPQGAIRRKYKRYSRTQIRNQNEDESKQQGGESGGFTPPPNANPIISAANVIVAPLPSQEYFKDPKSREDRIVHDKVYFPDELPPAHYKKRELIKKVIYPNDRIYRVQERIARQWQETLTWRKVLVSIQPDSHNNIVVRRKFVNAFGWVVIDHLVKEHFGAEGKTQSVSDSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>C5DM53</accession>
    <name>C5DM53_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ATP synthase subunit 5, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0G06072g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATPase delta chain family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555301.1">
        <property type="nucleotide sequence ID" value="XM_002555255.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DM53"/>
    <dbReference type="FunCoup" id="C5DM53">
        <property type="interactions" value="746"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DM53"/>
    <dbReference type="GeneID" id="8293569"/>
    <dbReference type="KEGG" id="lth:KLTH0G06072g"/>
    <dbReference type="eggNOG" id="KOG1662">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085114_0_0_1"/>
    <dbReference type="InParanoid" id="C5DM53"/>
    <dbReference type="OMA" id="MVDNIQD"/>
    <dbReference type="OrthoDB" id="1262810at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.20">
        <property type="entry name" value="N-terminal domain of the delta subunit of the F1F0-ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01416">
        <property type="entry name" value="ATP_synth_delta_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026015">
        <property type="entry name" value="ATP_synth_OSCP/delta_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000711">
        <property type="entry name" value="ATPase_OSCP/dsu"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01145">
        <property type="entry name" value="ATP_synt_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11910">
        <property type="entry name" value="ATP SYNTHASE DELTA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00213">
        <property type="entry name" value="OSCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00125">
        <property type="entry name" value="ATPASEDELTA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47928">
        <property type="entry name" value="N-terminal domain of the delta subunit of the F1F0-ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR24864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="206" mass="21961" checksum="62F93ADDB6F12ACF" modified="2009-07-28" version="1">MFSKLFTRSLATASKSVKPPIQLFGLDGTYATALFTAAAKTTSVEAASKSLSQLTETVTKDSKLTSILANPALSANDRAIVVDTLAKSQPNIDQSVANLLKVLGENNRLSLFPDVSIQFTKLTDAYNGLIQATVTTAQPLDSKLFKRVEKALAGSKLVGQGKTLKLENVVDPEIQGGLIVEVADRTVDLSISSKINKLNQLLKEAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMG4</accession>
    <name>C5DMG4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2-methoxy-6-polyprenyl-1,4-benzoquinol methylase, mitochondrial</fullName>
            <ecNumber evidence="4">2.1.1.201</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Ubiquinone biosynthesis methyltransferase COQ5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">COQ5</name>
        <name evidence="5" type="ordered locus">KLTH0G08690g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Methyltransferase required for the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a 2-methoxy-6-(all-trans-polyprenyl)benzene-1,4-diol + S-adenosyl-L-methionine = a 5-methoxy-2-methyl-3-(all-trans-polyprenyl)benzene-1,4-diol + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28286"/>
            <dbReference type="Rhea" id="RHEA-COMP:10858"/>
            <dbReference type="Rhea" id="RHEA-COMP:10859"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:84166"/>
            <dbReference type="ChEBI" id="CHEBI:84167"/>
            <dbReference type="EC" id="2.1.1.201"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of a multi-subunit COQ enzyme complex, composed of at least COQ3, COQ4, COQ5, COQ6, COQ7 and COQ9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Mitochondrion inner membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
            <orientation evidence="4">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily. MenG/UbiE family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.201" evidence="4"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555412.1">
        <property type="nucleotide sequence ID" value="XM_002555366.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMG4"/>
    <dbReference type="FunCoup" id="C5DMG4">
        <property type="interactions" value="510"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMG4"/>
    <dbReference type="GeneID" id="8293687"/>
    <dbReference type="KEGG" id="lth:KLTH0G08690g"/>
    <dbReference type="eggNOG" id="KOG1540">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037990_0_1_1"/>
    <dbReference type="InParanoid" id="C5DMG4"/>
    <dbReference type="OMA" id="MNDVMSM"/>
    <dbReference type="OrthoDB" id="6329284at2759"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031314">
        <property type="term" value="C:extrinsic component of mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008425">
        <property type="term" value="F:2-methoxy-6-polyprenyl-1,4-benzoquinol methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000133">
        <property type="entry name" value="2-methoxy-6-polyprenyl-1,4-benzoquinol methylase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01813">
        <property type="entry name" value="MenG_UbiE_methyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004033">
        <property type="entry name" value="UbiE/COQ5_MeTrFase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023576">
        <property type="entry name" value="UbiE/COQ5_MeTrFase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01934">
        <property type="entry name" value="MenG_MenH_UbiE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591:SF24">
        <property type="entry name" value="2-METHOXY-6-POLYPRENYL-1,4-BENZOQUINOL METHYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01209">
        <property type="entry name" value="Ubie_methyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51608">
        <property type="entry name" value="SAM_MT_UBIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01183">
        <property type="entry name" value="UBIE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01184">
        <property type="entry name" value="UBIE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="binding site" evidence="4">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="175"/>
            <end position="176"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR24975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="303" mass="33873" checksum="D307C3862DFB9D9B" modified="2009-07-28" version="1">MYHTRIASGSALRAGIMARSSKCALLSSSRCLHAGMLLRKEGPAETTHFGSKTVAKEEKEKLVGSVFSNVAAKYDVMNDVMSMGVHRLWKDHFINKLDAGKRPNSKEPLHFIDVAGGSGDIAFGLLDHAEHKFHDTESTMHIVDINADMLKEGEKRALNQGKYYNDPRVKFLVQNGETLDQIESNSKDVYTVSFGIRNFTDIQAGLNTAYRVLKPGGIFYCLEFSKVDNPLVDLVYQQWSKVLPVMGSLVANDYDSYQYLVESIQRFPQPEEFKAMIEKAGFESAGYEKLTFGVCAIHWGVKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMG9</accession>
    <name>C5DMG9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0G08822p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0G08822g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555417.1">
        <property type="nucleotide sequence ID" value="XM_002555371.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMG9"/>
    <dbReference type="FunCoup" id="C5DMG9">
        <property type="interactions" value="217"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMG9"/>
    <dbReference type="GeneID" id="8293693"/>
    <dbReference type="KEGG" id="lth:KLTH0G08822g"/>
    <dbReference type="eggNOG" id="ENOG502QTPN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035516_0_0_1"/>
    <dbReference type="InParanoid" id="C5DMG9"/>
    <dbReference type="OMA" id="IEYERYG"/>
    <dbReference type="OrthoDB" id="3976380at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="420"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="449"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="229"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="466"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR24980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="544" mass="60768" checksum="BDA92C79D305EE07" modified="2009-07-28" version="1">MSSKKIIGGAAMVAAGYALYEYQVQRQQRHLNTGALTQAPHKDAHLFEKKGGEAGSKLDQVTNQARDQVAGWAHDADRKVSSTMADVERAKANSSKWIGDQLGEAQEAVTDRRDKYLERSGELNAIVEETNERKREGQNRISRLVTDARDTISADIRNIKDGVSEDASSIKEAFVGAKKGTQDTADSLKRQASDVESDIKNKAGNARDTAVEAKESIFNWGFSKAEKAKAVAIQEYDQANKHFNELRDKYNSEKGLFSSGDQELKKQVDEASKKLEEYKKKLEDASSRYAKYTNDNINELSDKLEQEDRKLRKKGFFQWLRGDTSGSDKSKAHDVDEVATKSVVGWGETAEALAREELDELVRNKKIGRSEAQRRLDELKKIKDEGWFTYKGRNDEALAKRAAKALEGWGETASELAQEEYEEARRKTSNFNAPKTISDAVDTAKEKLDAAKKNLDENASGLWSSGKEKSEELRQKAQQEYEQAQKDYSSSLQSLSEWSEKAKGKFWSGADTALDATKSTADTLHAKAKEGLNSAQEYAKDKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMJ5</accession>
    <name>C5DMJ5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Nucleolar protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0G09460g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555443.1">
        <property type="nucleotide sequence ID" value="XM_002555397.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMJ5"/>
    <dbReference type="SMR" id="C5DMJ5"/>
    <dbReference type="FunCoup" id="C5DMJ5">
        <property type="interactions" value="844"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMJ5"/>
    <dbReference type="GeneID" id="8293721"/>
    <dbReference type="KEGG" id="lth:KLTH0G09460g"/>
    <dbReference type="eggNOG" id="KOG1122">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005316_3_2_1"/>
    <dbReference type="InParanoid" id="C5DMJ5"/>
    <dbReference type="OMA" id="PIGSWTK"/>
    <dbReference type="OrthoDB" id="427002at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009383">
        <property type="term" value="F:rRNA (cytosine-C5-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000470">
        <property type="term" value="P:maturation of LSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000192">
        <property type="entry name" value="NOP2p rRNA m5C methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1170:FF:000001">
        <property type="entry name" value="Ribosomal RNA methyltransferase Nop2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1170">
        <property type="entry name" value="Sun protein, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049560">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011023">
        <property type="entry name" value="Nop2p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023273">
        <property type="entry name" value="RCMT_NOP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018314">
        <property type="entry name" value="RsmB/NOL1/NOP2-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00446">
        <property type="entry name" value="nop2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807:SF30">
        <property type="entry name" value="28S RRNA (CYTOSINE(4447)-C(5))-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02012">
        <property type="entry name" value="RCMTNOP2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01153">
        <property type="entry name" value="NOL1_NOP2_SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="7 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent MTase RsmB/NOP-type" evidence="12">
        <location>
            <begin position="272"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="167"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="616"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="19"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="48"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="75"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="88"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="98"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="625"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="10">
        <location>
            <position position="489"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="364"/>
            <end position="370"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="388"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="415"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="432"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00082314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR25006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="637" mass="71457" checksum="011CA7A42DBA92DE" modified="2009-07-28" version="1">MGSRRHKNKQGAPPSLDEFQAKKSNKSEHKGKSRSEDVDGKKHKSKRRAENDTKSGNKRSKTERVEKTKSKEPEQTEEEELPEVDLEELSKARKTLFDDEEDEGQEEDGADEFDEDLKDEFDLEQEYEGEDDDDEGGRPVFSDDEGVDMEDLNAANMEALSMKLDQEQALEEEEAEKELAEAAQLQPRAEILPTEEEEEALRDRPTDLNAVRTRMIEIVKVLEDFKNLGAEGRSRSEYVDRLLKDICEYFGYSQFLAEKLFNLFSPTEALEFFEANEVSRPVTIRTNSLKTRRRDLAQTLVNRGVNLKPIGPWTKVGLQIFDSQVPIGATPEYLAGHYILQAASSFLPVVALDPHENERILDMAAAPGGKTTYISAMMKNTGCVFANDANKARTKSLIANIHRLGCTNTIVCNYDAREFPKVIGGFDRILLDAPCSGTGVIAKDQSVKVSRTDKDFMQIPHLQKQLLLSAIDSVDANSKTGGVIVYSTCSVAVEENEAVVDYALRKRPNVKLVDAGLAIGKEAFVSYRGKKFHPSVKLARRYYPHVYNVDGFFVAKFQKIAPSKHDDNQANAREKENAARQEALEEGIIHDDFADFGNEEDKKYIEKSRKVSLLKKGIDPKATKKNQGSNSTPNKSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMV4</accession>
    <name>C5DMV4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0G11924p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0G11924g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555552.1">
        <property type="nucleotide sequence ID" value="XM_002555506.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMV4"/>
    <dbReference type="FunCoup" id="C5DMV4">
        <property type="interactions" value="816"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMV4"/>
    <dbReference type="GeneID" id="8293832"/>
    <dbReference type="KEGG" id="lth:KLTH0G11924g"/>
    <dbReference type="eggNOG" id="KOG4018">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084528_0_0_1"/>
    <dbReference type="InParanoid" id="C5DMV4"/>
    <dbReference type="OMA" id="QWDEHKK"/>
    <dbReference type="OrthoDB" id="277175at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="CDD" id="cd23824">
        <property type="entry name" value="RWD_ScGIR2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040213">
        <property type="entry name" value="GIR2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006575">
        <property type="entry name" value="RWD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12292">
        <property type="entry name" value="RWD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05773">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00591">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50908">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RWD" evidence="2">
        <location>
            <begin position="9"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR25115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="250" mass="29170" checksum="C80400DAF8189AAE" modified="2009-07-28" version="1">MDYKDEQSQELEVLESIYPDEFAVQSDEYPKVQFSVSLPLDLVPLESSSFTQDSINMEHHLVLNFKLPETYPETVPEITIECEQERKANVHDDSEDSEEDEEVEYDEHGNPVESKLANLPDQVQFDEYVKVLESQVESQASEDMLIGMQMCFALVSWIKESCEHHFQERLAELEREHERKLLDREAEEQKKFRGTKVTSESYLEWRAKFREETGLNERDAARKVEAHSGRLTGRQIFEQGLDGSEDVEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMX7</accession>
    <name>C5DMX7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Acyl-protein thioesterase 1</fullName>
            <ecNumber evidence="2">3.1.2.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Palmitoyl-protein hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0G12474g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Hydrolyzes fatty acids from S-acylated cysteine residues in proteins with a strong preference for palmitoylated G-alpha proteins over other acyl substrates. Mediates the deacylation of G-alpha proteins such as GPA1 in vivo, but has weak or no activity toward palmitoylated Ras proteins. Has weak lysophospholipase activity in vitro; however such activity may not exist in vivo.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>S-hexadecanoyl-L-cysteinyl-[protein] + H2O = L-cysteinyl-[protein] + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19233"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="3.1.2.22"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily. AB hydrolase 2 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.22" evidence="2"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555575.1">
        <property type="nucleotide sequence ID" value="XM_002555529.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMX7"/>
    <dbReference type="FunCoup" id="C5DMX7">
        <property type="interactions" value="501"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMX7"/>
    <dbReference type="GeneID" id="8293857"/>
    <dbReference type="KEGG" id="lth:KLTH0G12474g"/>
    <dbReference type="eggNOG" id="KOG2112">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049413_3_8_1"/>
    <dbReference type="InParanoid" id="C5DMX7"/>
    <dbReference type="OMA" id="WYDILAM"/>
    <dbReference type="OrthoDB" id="2418081at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008474">
        <property type="term" value="F:palmitoyl-(protein) hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050565">
        <property type="entry name" value="LYPA1-2/EST-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003140">
        <property type="entry name" value="PLipase/COase/thioEstase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10655:SF17">
        <property type="entry name" value="LYSOPHOSPHOLIPASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10655">
        <property type="entry name" value="LYSOPHOSPHOLIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02230">
        <property type="entry name" value="Abhydrolase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0719">Serine esterase</keyword>
    <feature type="domain" description="Phospholipase/carboxylesterase/thioesterase" evidence="10">
        <location>
            <begin position="7"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR25138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="229" mass="24984" checksum="1CA00F980D3BEAD6" modified="2009-07-28" version="1">MSVINAIKIASKAQPAKQSLIFLHGLGDTGSGWSFLAELLQQDPAFRYTNFIFPNAPVMGITVNGNYPMPAWFDIRSWDNVQSQADVAGFLKSLHVVERLVDEQIQNGVNPQNIVVGGFSQGAALALGSAVTLPTKIAGFVALSGFSIINDKLLELKSPANSDTPIFHGHGDQDTVIPLKYGHSVEQFFTKYCGISNYTMNVYSGMEHSASPEEIEDLVKFLKSILRLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMX9</accession>
    <name>C5DMX9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0G12518p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0G12518g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic/archaeal PrmC-related family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555577.1">
        <property type="nucleotide sequence ID" value="XM_002555531.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMX9"/>
    <dbReference type="FunCoup" id="C5DMX9">
        <property type="interactions" value="393"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMX9"/>
    <dbReference type="GeneID" id="8293859"/>
    <dbReference type="KEGG" id="lth:KLTH0G12518g"/>
    <dbReference type="eggNOG" id="KOG3191">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018398_6_0_1"/>
    <dbReference type="InParanoid" id="C5DMX9"/>
    <dbReference type="OMA" id="EWDDWME"/>
    <dbReference type="OrthoDB" id="406152at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035657">
        <property type="term" value="C:eRF1 methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052190">
        <property type="entry name" value="Euk-Arch_PrmC-MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004557">
        <property type="entry name" value="PrmC-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007848">
        <property type="entry name" value="Small_mtfrase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00537">
        <property type="entry name" value="hemK_rel_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45875">
        <property type="entry name" value="METHYLTRANSFERASE N6AMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45875:SF1">
        <property type="entry name" value="METHYLTRANSFERASE N6AMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05175">
        <property type="entry name" value="MTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase small" evidence="5">
        <location>
            <begin position="39"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR25140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="220" mass="24861" checksum="AAD176CDFADCFCFA" modified="2009-07-28" version="1">MLPTPHVSCDYDKVYEPSEDSFLILDALEKEQAFLRTYFRSKPTVVCEVGCGSGIVTTFMMQNDIPNEHAIYLPTDVNPWAMDSMVESASLNNCCTKMLSPIRTNLTSSIRPREIDFLVFNPPYVPAESVPYMPLDSEANEGDWLDLALLGGDDGMAITWKLLDSLDKIMAPGGIAYILFCARNHPEKVVKTMSTRGWKCELTEHRKAGWEVLSVYKFYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>C5DN20</accession>
    <name>C5DN20_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0G13486p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0G13486g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555618.1">
        <property type="nucleotide sequence ID" value="XM_002555572.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DN20"/>
    <dbReference type="FunCoup" id="C5DN20">
        <property type="interactions" value="540"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DN20"/>
    <dbReference type="GeneID" id="8293901"/>
    <dbReference type="KEGG" id="lth:KLTH0G13486g"/>
    <dbReference type="eggNOG" id="KOG0236">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003182_8_1_1"/>
    <dbReference type="InParanoid" id="C5DN20"/>
    <dbReference type="OMA" id="EPSAKEW"/>
    <dbReference type="OrthoDB" id="288203at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008271">
        <property type="term" value="F:secondary active sulfate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07042">
        <property type="entry name" value="STAS_SulP_like_sulfate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="entry name" value="STAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018045">
        <property type="entry name" value="S04_transporter_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001902">
        <property type="entry name" value="SLC26A/SulP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00815">
        <property type="entry name" value="sulP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11814">
        <property type="entry name" value="SULFATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SpoIIaa-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01130">
        <property type="entry name" value="SLC26A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="232"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="263"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="315"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="354"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="451"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="489"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="538"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="564"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="domain" description="STAS" evidence="7">
        <location>
            <begin position="759"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="10"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="68"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR25181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="902" mass="97968" checksum="F922304C9C36B25E" modified="2009-07-28" version="1">MSNFQSSPGSKRESSFEVSRELSNDAPIDLDDLDAEYGQFKNAEHSDATAGRDLTSEYGAQFAAEFGGEPGSGSGSGSGFPEAKPVAPAAPGNVPSALYEFHERPVTVGEFYSQNLRSTFTLATFTNYLRSLLPIMRWLPHYNARWLYQDLVAGITVGCVLVPQSMSYAQIATLSPQYGLYSSFVGAFIYSFFATSKDVCIGPVAVMSLQTAKAISHVVSSLPEDTEITSPMIATALALLCGIISLGLGVLRLGFLVELISSTAVAGFMTGSALNIIAGQVPALMGYNKLVNTRTSTYKVIINSLRHLPDTKLDAVFGLVPLVILYVWKWGCSTGGPRLVQRYGSRRSRMWDNVFLYTQALRNAVVIVVFTAIAWGMSHRALKEGGSARISLLGTVPSGLKDVGVMKVPSGLLSKIAPELPASVIVLVLEHIAISKAFGRVNDYRVVPDQELIAIGATNLIGTFFNAYPATGSFSRSALKAKCNVSTPLSGLFSGACVLLAIYCLTSAFKFIPKATLSAVIIHAVSDLIASYKTTWSFWRLSPPDLVCFLVTVVITVFSSIENGIYFAMCWSVAVLLFRTAFPAGKFLGRVQIAEARVTNSGAGAGVGMSSGNSAGTAVNREKERETTFTAVSVSSESLSHTAPGKAKAANSAGTDKLGKVEGHVTDGGSPRAPRFHTKWVPFDRYTRELNPEVFVAPPPPGVIVFRPSESWTYVNCSRQYDAIFDEVVRLTRRGRPQIVAKSSSRPWNDPGEWHPPKFLRKLFKSSSEDLENRAVARDERPVLRVIAMDWSQVAQVDSTGLQTLQDLRKAVNKYADRQVEFHFAGIIEPWVKRGLINSGFGTVNDEFADESLLVGHKSCHIARSAEPTEDEESRLAHPATGTNLPFFHLELPDFSEWDYSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5DN78</accession>
    <name>C5DN78_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0G14806p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0G14806g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555676.1">
        <property type="nucleotide sequence ID" value="XM_002555630.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DN78"/>
    <dbReference type="FunCoup" id="C5DN78">
        <property type="interactions" value="166"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DN78"/>
    <dbReference type="GeneID" id="8293961"/>
    <dbReference type="KEGG" id="lth:KLTH0G14806g"/>
    <dbReference type="eggNOG" id="ENOG502SDW5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_122989_1_0_1"/>
    <dbReference type="InParanoid" id="C5DN78"/>
    <dbReference type="OMA" id="EGATRPW"/>
    <dbReference type="OrthoDB" id="274752at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019711">
        <property type="entry name" value="ATP_synth_F0_suH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28207">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT H, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28207:SF1">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT H, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10775">
        <property type="entry name" value="ATP_sub_h"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR25239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="113" mass="12697" checksum="7B6D27E3B85F2837" modified="2009-07-28" version="1">MFLQAFKTAVPRSVRTFASTRVRSNVIQDLYLREIKSVKLQPVSAKDAEGSVKAWTAPQAPKLPELEAQGPEALKAYAQEDVHVTKTEAESAAESEEQDWLVIEDIEEENHHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>C5DN85</accession>
    <name>C5DN85_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0G14960p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0G14960g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555683.1">
        <property type="nucleotide sequence ID" value="XM_002555637.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DN85"/>
    <dbReference type="FunCoup" id="C5DN85">
        <property type="interactions" value="258"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DN85"/>
    <dbReference type="GeneID" id="8293968"/>
    <dbReference type="KEGG" id="lth:KLTH0G14960g"/>
    <dbReference type="eggNOG" id="ENOG502RA7D">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_597417_0_0_1"/>
    <dbReference type="InParanoid" id="C5DN85"/>
    <dbReference type="OMA" id="EPQVWCK"/>
    <dbReference type="OrthoDB" id="419537at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030896">
        <property type="term" value="C:checkpoint clamp complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044778">
        <property type="term" value="P:meiotic DNA integrity checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033314">
        <property type="term" value="P:mitotic DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031573">
        <property type="term" value="P:mitotic intra-S DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.70.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007150">
        <property type="entry name" value="HUS1/Mec3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12900:SF0">
        <property type="entry name" value="CHECKPOINT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12900">
        <property type="entry name" value="MITOTIC AND DNA DAMAGE CHECKPOINT PROTEIN HUS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04005">
        <property type="entry name" value="Hus1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR25246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="417" mass="46418" checksum="7A165F6F7726B7C8" modified="2009-07-28" version="1">MKLKIIVNGSQSPEDFKLLKSTVATVASLRKTAVLRFTSERLVIVSSPSSTASGSSVLHGDKGQLWCTIPRDAFTFYSVVSARDLNTIAMECSCDMLHSILKKYDKTINQGNEGDMIIKLQSMPEWNANTSQASDGKNGSAARINPMCALGVTFEEVVYTSTDANASAKSGGGNSGGIKTISHSFKVPARLLFRTQDAKIQEPMINYTQLMMYRLPAPQGEWGRGFQNFLRRVERYSNVNNIKLSAKKFWQQEERTASQETAGDHAFKIVVNELDWYLEICWNGPLDAMVQPENIQEDVQDSRAPAGADPTHAADQSLFIEDSASNTNDPLDLPIENTPEAPQDVQPSKHEVIIRCKDWKVCSKLYDAFEEVVLAISHDESCVFHCTLPRGNVEDENGERAREYGQVIYYMARAKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNR4</accession>
    <name>C5DNR4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein transport protein BOS1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0G19206g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">SNARE required for protein transport between the ER and the Golgi complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Endomembrane system</location>
            <topology evidence="6">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the BOS1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555862.1">
        <property type="nucleotide sequence ID" value="XM_002555816.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNR4"/>
    <dbReference type="FunCoup" id="C5DNR4">
        <property type="interactions" value="78"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNR4"/>
    <dbReference type="GeneID" id="8294157"/>
    <dbReference type="KEGG" id="lth:KLTH0G19206g"/>
    <dbReference type="eggNOG" id="KOG3251">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078260_1_0_1"/>
    <dbReference type="InParanoid" id="C5DNR4"/>
    <dbReference type="OMA" id="FCWLVIH"/>
    <dbReference type="OrthoDB" id="158360at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027027">
        <property type="entry name" value="GOSR2/Membrin/Bos1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028865">
        <property type="entry name" value="Membrin-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="218"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="94"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00046280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR028865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR25425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="239" mass="26875" checksum="8DEE4B3FA4A2208F" modified="2009-07-28" version="1">MNALSNHATKQKTLLQRDLNKFEKELAVAPISLQGAIAATLVSLEKTIVQYKELLTRQFAGSSKEEEASKVKYQERLESLQQDLEAAKTRFQTLKKQCSSTNSREQLLKSSSNPFDEESTMSKRNTAGSPGLGHAGNASSGSQLPLFQGLQKEQSVFERGNAQLDYILEMGQQSLGDIIDQNAVLRKMEVQMTKSMQTLGVSNETINKINKRVFKDKFIFWIALALMLTGFYFVLKWLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5E1Z3</accession>
    <name>C5E1Z3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">6-phosphogluconolactonase</fullName>
            <ecNumber evidence="5">3.1.1.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0H00836g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>6-phospho-D-glucono-1,5-lactone + H2O = 6-phospho-D-gluconate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12556"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57955"/>
            <dbReference type="ChEBI" id="CHEBI:58759"/>
            <dbReference type="EC" id="3.1.1.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 2/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. 6-phosphogluconolactonase subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.31" evidence="5"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555916.1">
        <property type="nucleotide sequence ID" value="XM_002555870.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E1Z3"/>
    <dbReference type="FunCoup" id="C5E1Z3">
        <property type="interactions" value="624"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E1Z3"/>
    <dbReference type="GeneID" id="8294228"/>
    <dbReference type="KEGG" id="lth:KLTH0H00836g"/>
    <dbReference type="eggNOG" id="KOG3147">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_729721_0_0_1"/>
    <dbReference type="InParanoid" id="C5E1Z3"/>
    <dbReference type="OMA" id="YQLFEFE"/>
    <dbReference type="OrthoDB" id="432544at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017057">
        <property type="term" value="F:6-phosphogluconolactonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01400">
        <property type="entry name" value="6PGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1360:FF:000005">
        <property type="entry name" value="6-phosphogluconolactonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005900">
        <property type="entry name" value="6-phosphogluconolactonase_DevB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039104">
        <property type="entry name" value="6PGL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006148">
        <property type="entry name" value="Glc/Gal-6P_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01198">
        <property type="entry name" value="pgl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11054">
        <property type="entry name" value="6-PHOSPHOGLUCONOLACTONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11054:SF24">
        <property type="entry name" value="6-PHOSPHOGLUCONOLACTONASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01182">
        <property type="entry name" value="Glucosamine_iso"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucosamine/galactosamine-6-phosphate isomerase" evidence="8">
        <location>
            <begin position="132"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR30054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="379" mass="41175" checksum="E64E8D673FD4F0BA" modified="2009-07-28" version="1">MCACAWERKKRRGAGAGAGRASLRTGRDITLDGMPCTHVTCAMPNGCAPARDVYHTVRGVSLHTDSQDPQRHLARALPSPPTNATRAIYRRPALPYIKTRRPRAAGLHAPTALHAHFLPPPVPMVPVYTYDDSAALSRDLALYILAQQDAALQRGDRFNLAVSGGSLLKALRAGLVEDAELRARANWARWHVFFCDERIVPLEHADSNYGAFKAQVLDPLLQHDGVLGPTCYAINESLVGLGENDRLAAEYESLLPAQFDLILLGCGPDGHTCSLFPGDTHSYLLDANTDRRVAWCHSSPKPPADRITFTLPTLASAHALCFVAEGAAKQPVLQRILQSSPDTSLPAALVTDRFAQRVSWFVDSAAVQDLSVPAVTYNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5E1Z5</accession>
    <name>C5E1Z5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial</fullName>
            <ecNumber evidence="10">6.2.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Succinyl-CoA synthetase beta chain</fullName>
            <shortName evidence="10">SCS-beta</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0H00880g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of ATP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>succinate + ATP + CoA = succinyl-CoA + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17661"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="10">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Heterodimer of an alpha and a beta subunit. The beta subunit determines specificity for GTP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10 12">Belongs to the succinate/malate CoA ligase beta subunit family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.5" evidence="10"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555918.1">
        <property type="nucleotide sequence ID" value="XM_002555872.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E1Z5"/>
    <dbReference type="FunCoup" id="C5E1Z5">
        <property type="interactions" value="773"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E1Z5"/>
    <dbReference type="GeneID" id="8294230"/>
    <dbReference type="KEGG" id="lth:KLTH0H00880g"/>
    <dbReference type="eggNOG" id="KOG2799">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037430_0_2_1"/>
    <dbReference type="InParanoid" id="C5E1Z5"/>
    <dbReference type="OMA" id="ITACDEV"/>
    <dbReference type="OrthoDB" id="1552at2759"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00999"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042709">
        <property type="term" value="C:succinate-CoA ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004775">
        <property type="term" value="F:succinate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006104">
        <property type="term" value="P:succinyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
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    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000002">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.261:FF:000001">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000004">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00558">
        <property type="entry name" value="Succ_CoA_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013650">
        <property type="entry name" value="ATP-grasp_succ-CoA_synth-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017866">
        <property type="entry name" value="Succ-CoA_synthase_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="SUCC_ACL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005809">
        <property type="entry name" value="Succ_CoA_ligase-like_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001913">
        <property type="entry name" value="PRK00696.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01016">
        <property type="entry name" value="sucCoAbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11815:SF1">
        <property type="entry name" value="SUCCINATE--COA LIGASE [ADP-FORMING] SUBUNIT BETA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11815">
        <property type="entry name" value="SUCCINYL-COA SYNTHETASE BETA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08442">
        <property type="entry name" value="ATP-grasp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001554">
        <property type="entry name" value="SucCS_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01217">
        <property type="entry name" value="SUCCINYL_COA_LIG_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="2 10" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="ATP-grasp" evidence="13">
        <location>
            <begin position="32"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="76"/>
            <end position="78"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared with subunit alpha</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="353"/>
            <end position="355"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared with subunit alpha</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00063570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR30056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="418" mass="45316" checksum="247E3E7D0F4A849F" modified="2009-07-28" version="1">MLSRTIAANQLKNSLRASKQIRNLSIHEYRSAELLRQYGVGTPAGEAAFEPQQAYDIAKKLGHDALVVKAQALTGGRGKGHFDTGLKSGVHMIESAEQARDLAKQMLKHRLITKQSGAKGKPVSAVYVVERVNAKHEAYLSILMDRQSKKAMIICSSEGGMNIEEVAAKNPDAIKKFTVTESEGLTAETAKQISKSLGFSAEAEADAADAVTKLYKIFKEKDASQIEINPLSEVEGQDTKVMCMDAKFGFDDNASFRQEEVYSWRDLSQEDADEVVAKKSDLNFVKLQGNIGCLVNGAGLAMATMDVIKLYGGDPANFLDCGGGATPETIETAFKLILSNEKVDGIFVNIFGGIVRCDFVAQGLVSATKNLAVSVPIVARLQGTNLEEGRRIIEESGLKIYSFDELDPAAQKVVELTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5E237</accession>
    <name>C5E237_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Tubulin gamma chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0H01870g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Tubulin is the major constituent of microtubules, protein filaments consisting of alpha- and beta-tubulin heterodimers. Gamma-tubulin is a key component of the gamma-tubulin ring complex (gTuRC) which mediates microtubule nucleation. The gTuRC regulates the minus-end nucleation of alpha-beta tubulin heterodimers that grow into microtubule protafilaments, a critical step in centrosome duplication and spindle formation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the tubulin family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555960.1">
        <property type="nucleotide sequence ID" value="XM_002555914.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E237"/>
    <dbReference type="FunCoup" id="C5E237">
        <property type="interactions" value="742"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E237"/>
    <dbReference type="GeneID" id="8294273"/>
    <dbReference type="KEGG" id="lth:KLTH0H01870g"/>
    <dbReference type="eggNOG" id="KOG1374">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015718_1_0_1"/>
    <dbReference type="InParanoid" id="C5E237"/>
    <dbReference type="OMA" id="HRYISIL"/>
    <dbReference type="OrthoDB" id="10249382at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000930">
        <property type="term" value="C:gamma-tubulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02188">
        <property type="entry name" value="gamma_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002454">
        <property type="entry name" value="Gamma_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
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        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
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        <property type="entry name" value="Tubulin_C"/>
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        <property type="entry name" value="Tubulin_CS"/>
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        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
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        <property type="entry name" value="TUBULIN"/>
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        <property type="entry name" value="Tubulin_C"/>
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    <dbReference type="PRINTS" id="PR01164">
        <property type="entry name" value="GAMMATUBULIN"/>
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    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
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    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
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    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
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    <dbReference type="PROSITE" id="PS00227">
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5 9" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="10">
        <location>
            <begin position="51"/>
            <end position="247"/>
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            <begin position="249"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00018848"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
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    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00864"/>
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        <source>
            <dbReference type="SMART" id="SM00865"/>
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    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR30098.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
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    </evidence>
    <sequence length="468" mass="52219" checksum="0F4C067621562FE7" modified="2009-07-28" version="1">MTGEIITLQIGQCGNQVGKQFWSQLIKEHGISRDGQSLSTNESDTVEEDDPTIFFKQNDSNRYTPRALLFDLEPSAIADVQNSFPGLFNERNVWVSKDELGAGNAWSKGYDHGLENQEEFINMVDKEIDATESFEGFQLLHSVAGGTGSGLGSNFLEALSDRYHKKIITTYSVFPSSESEVVIQPYNTILTLRRLIEHSDASVVFDNKALLNLATRVFRDRNTSYEQTNQLIASVLSSITNSLRFPSYMYNSLPSIFSTLVPTADLHFLVPSFTPFTSDFIPGVRDFKKSSAYDVILDLFDKSNSMVLRNNDTPVYLSIYNSILGPTDQADITRAILKSQQRIKFPPWSSSALHVNVGKKSAYNVTPDSDKVSGMMLANTTSVNALLQTACSSFDVLFKRGAFLHMYQDGKMFQNGAEEFIESREVVQSCINEYYAAEQETFMDDVLMEEENIVGNGGMLDADGDDII</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2A6</accession>
    <name>C5E2A6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">KLTH0H03410p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0H03410g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_250352_0_0_1"/>
    <dbReference type="InParanoid" id="C5E2A6"/>
    <dbReference type="OMA" id="GFDQVCK"/>
    <dbReference type="OrthoDB" id="1678912at2759"/>
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        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0005634">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:chromatin remodeling"/>
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    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="PHD_Ecm5p_Lid2p_like"/>
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        <property type="entry name" value="ARID DNA-binding domain"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ARID_dom"/>
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        <property type="entry name" value="ARID_dom_sf"/>
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        <property type="entry name" value="JmjC_dom"/>
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        <property type="entry name" value="JmjN"/>
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        <property type="entry name" value="Lys_sp_deMease-like_dom"/>
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        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
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        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
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        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
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        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
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        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694:SF113">
        <property type="entry name" value="PROTEIN JUMONJI"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ARID"/>
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        <property type="entry name" value="PLU-1"/>
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        <property type="entry name" value="BRIGHT"/>
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        <property type="entry name" value="FYVE/PHD zinc finger"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="JMJN"/>
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    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
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    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="JmjN" evidence="10">
        <location>
            <begin position="111"/>
            <end position="152"/>
        </location>
    </feature>
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        <location>
            <begin position="179"/>
            <end position="277"/>
        </location>
    </feature>
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        <location>
            <begin position="491"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="8">
        <location>
            <begin position="1262"/>
            <end position="1314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
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            <begin position="572"/>
            <end position="591"/>
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            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1470" mass="166410" checksum="A9D3EECA640C81B1" modified="2009-07-28" version="1">MTLEPLPHMPPVGKWQPSTGSNAPLAAALPLAAGKTLPTTSRFKSSLEATPSPLSLDQHSPHLYTQHRIRPNAPFDIAHIKSKRLVQTPEPACQTLKTDSGWSYTLESGSVPCFRIPRSEIPDPITFYHQIEDVGRQYGAVKLQIIQSSPAPVTPFALNTELFWFKARRQSLCSFQEETRRKLDLHRRLYKYHSSRKSDTGRQLFSKIPSIDKRPLDLYRLRECVQLRGGFQEVCHKKLWAQIGRELGYSGRIMSSLSTSLRSAYLKVFSEFDKEEERQRASASAATTTVPTKIHIILPSSTEDNHRDPSSKKRSLNEASISLENAVKKHKASKVQAYQVAGSAAEFSRLRDILRYKGFFTNFENLTEPRKGLTKPSPSTLPGYQFTFWKSSSEIYDRSAHETRNAPIYNLRQYYEKAQKHFDSVAALYQQRSSLVLEKSDSVNLRDFEKLFFDILSDESACCNVDTGINLPSSVHGSGFPTVSKSTTNGLDTTSAWNLNNIPLSSDSVLQFMDADFGSHLDTRLDLGMLFSVKGWSIEDNFLPNIDYHHAGSSKLWYVVPPEDMKRFESLVETSKKNQQSPGPEVSNDPVDKSFLNSEVYQCYLDTCPQHQVSTLPPRINTHTIGQFREVTACTLPDDWQFHPEKLKANSIKVLRIVQDPGCYILKFPRTYSTSISCGFSISENALFAPASWLESALESEKWLSRNGLLPAIQTFQLLFNIVKESGDKKLASKARLLLTGLASEELAAREELARRLSDRFTIESNVFDFISDQTLEPTGASKVLLTSAKDCFTLSLRQFLDNLTCKEEKLLIFGRDLQQDGISISLHEFYSNEELEALLQEDHACTAAAAIFSEKDASQGQVEKPDRDVLVSYDSLVNEELKGQRVPLAKINLFCEALSQESNSQAHQLRRSLKNARSTASRCNQMLAKINKEKRGMKFQGFGRGFALKELAAAPFHYTVAELHNLYLELEGSSIAFPEMSKVFELCHCVESFQLDAQNAIKINDLSRMKETYLQGWSLGIRSKYHDLLARKICEEMWLSTYEKTIIEHEIIKEDELGYYTPSYLLPYLKYGIKFVSDALHREKLEQVRNTILLSQETGYELGQLFKKKSSKIPVDRLSSILDTLRQYPFLVEPRLRETLEAIMDTFGKHREGMSSSFDKLDVNHEHYRLIAGSVNARPLFELNLIGKFDGSCDDKRLTLSEVPNRSIFSKHIKDCRLWLQKVNKLVLRKPLWAKIHSSTQLCFANNGDKLTTKSQPDEEPVYCFCRQGDSGSKMVACEVCGEWYHATCINKGKWSLVSNENTVFVCPICCPSNFPDFGLVDFSQIESMAMASLKLKVIPDRRFFGQFFEIFKMSASFKHTMSCDLLKKDGALRGDLSMAQIKFYLRKLVGAGVRMPREISALQRACQEHDANVMLKFAQRNIKVVTGYEATGSREASEAQPDQVVIKSEGAMVISPEIKPEGNSDMMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2G6</accession>
    <name>C5E2G6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0H04796p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0H04796g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556089.1">
        <property type="nucleotide sequence ID" value="XM_002556043.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2G6"/>
    <dbReference type="FunCoup" id="C5E2G6">
        <property type="interactions" value="1168"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2G6"/>
    <dbReference type="GeneID" id="8294403"/>
    <dbReference type="KEGG" id="lth:KLTH0H04796g"/>
    <dbReference type="eggNOG" id="KOG0896">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063065_4_0_1"/>
    <dbReference type="InParanoid" id="C5E2G6"/>
    <dbReference type="OMA" id="GPESCSY"/>
    <dbReference type="OrthoDB" id="6508832at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006301">
        <property type="term" value="P:DNA damage tolerance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23807">
        <property type="entry name" value="UEV_UBE2V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000026">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="2">
        <location>
            <begin position="5"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR30227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="138" mass="15694" checksum="3DEC02EEBF4472C4" modified="2009-07-28" version="1">MSRVPRSFRLLEELEKGEKGMGPESCSYGLAHSDDITMTHWNGTILGPPHSNHENRIYSVAIECGEAYPDQPPKIRFLSKVNLPCVDQNTGEVKKEQFSTLRDWKRSYTMETLLLDLRKEMALPSNKKLPQPAEGTTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2I7</accession>
    <name>C5E2I7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0H05324p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0H05324g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 13 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556110.1">
        <property type="nucleotide sequence ID" value="XM_002556064.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2I7"/>
    <dbReference type="STRING" id="559295.C5E2I7"/>
    <dbReference type="GeneID" id="8294425"/>
    <dbReference type="KEGG" id="lth:KLTH0H05324g"/>
    <dbReference type="eggNOG" id="KOG0471">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006462_1_1_1"/>
    <dbReference type="InParanoid" id="C5E2I7"/>
    <dbReference type="OMA" id="LMYEWRD"/>
    <dbReference type="OrthoDB" id="1740265at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004556">
        <property type="term" value="F:alpha-amylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033934">
        <property type="term" value="F:glucan 1,4-alpha-maltotriohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004574">
        <property type="term" value="F:oligo-1,6-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004575">
        <property type="term" value="F:sucrose alpha-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000025">
        <property type="term" value="P:maltose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005987">
        <property type="term" value="P:sucrose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11333">
        <property type="entry name" value="AmyAc_SI_OligoGlu_DGase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000064">
        <property type="entry name" value="Oligo-1,6-glucosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.400.10:FF:000004">
        <property type="entry name" value="Oligo-1,6-glucosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000087">
        <property type="entry name" value="Oligo-1,6-glucosidase IMA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.400.10">
        <property type="entry name" value="Oligo-1,6-glucosidase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="GH13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045857">
        <property type="entry name" value="O16G_dom_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10357">
        <property type="entry name" value="ALPHA-AMYLASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10357:SF179">
        <property type="entry name" value="NEUTRAL AND BASIC AMINO ACID TRANSPORT PROTEIN RBAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0462">Maltose metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 13 catalytic" evidence="5">
        <location>
            <begin position="15"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00026248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR30248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="586" mass="67375" checksum="D6D13AF3829AF16D" modified="2009-07-28" version="1">MSAVDKWWKEAAVYQIYPASFYDSNGDGWGDLPGITAKLPYLRQLGVDALWICPFYDSPQQDMGYDISDYEKVWPRYGTNEDCYQLIREAHAAGIRVVVDLVINHCSSEHAWFRESRSSKTSAKRDWFIWRPAKGFTADGKPIPPNNWQSFFGGSAWEWDERTQEFYLRLFASGQPDFNWELSEVRKAIYESAAGFWLDHGVDGFRIDTAGLYSKIGGLPDAEVRDATNEHQHPGHNTQNGPRIHEFHKEMRAFFDKRTAGRANEIFTVGEVGRCDLAGLLQYTSEQEREMSQLFNFAHTECGVGTHLRYHAQPFTLKEWKLGIAASFLFANRTDSWSTVYLENHDQPRSVTRFGDDSPAWRQVSAKLLAILEVALTGTLYVYQGQELGQINNHGWTLDQYQDVDMITNRDIIAKRYGKNSVEMAKFAQGVALLSRDHSRTPFPWTSREPDASFSAVCQSGVKPWISMNDSFRDGINAEDELKDPNSVFHFWRAALRLRKQHKDILVYGQGFEFFDLDNPDLFMFTKKSRDGAKTLFAALNFSSEDREFPLPDEDAAYKAFFGNYDLESAQERTLRPWEGRLFYVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2K2</accession>
    <name>C5E2K2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">5'-deoxynucleotidase</fullName>
            <ecNumber evidence="8">3.1.3.89</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0H05654g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the dephosphorylation of the nucleoside 5'-monophosphates deoxyadenosine monophosphate (dAMP), deoxycytidine monophosphate (dCMP), deoxyguanosine monophosphate (dGMP) and deoxythymidine monophosphate (dTMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2'-deoxyribonucleoside 5'-phosphate + H2O = a 2'-deoxyribonucleoside + phosphate</text>
            <dbReference type="Rhea" id="RHEA:36167"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:18274"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:65317"/>
            <dbReference type="EC" id="3.1.3.89"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the HDDC2 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.89" evidence="8"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556125.1">
        <property type="nucleotide sequence ID" value="XM_002556079.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2K2"/>
    <dbReference type="FunCoup" id="C5E2K2">
        <property type="interactions" value="515"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2K2"/>
    <dbReference type="GeneID" id="8294440"/>
    <dbReference type="KEGG" id="lth:KLTH0H05654g"/>
    <dbReference type="eggNOG" id="KOG3197">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039453_2_0_1"/>
    <dbReference type="InParanoid" id="C5E2K2"/>
    <dbReference type="OMA" id="TWRLCLM"/>
    <dbReference type="OrthoDB" id="10254258at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002953">
        <property type="term" value="F:5'-deoxynucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009159">
        <property type="term" value="P:deoxyribonucleoside monophosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3210.10:FF:000011">
        <property type="entry name" value="HD domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039356">
        <property type="entry name" value="YfbR/HDDC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11845">
        <property type="entry name" value="5'-DEOXYNUCLEOTIDASE HDDC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11845:SF13">
        <property type="entry name" value="5'-DEOXYNUCLEOTIDASE HDDC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13023">
        <property type="entry name" value="HD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0170">Cobalt</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="13">
        <location>
            <begin position="76"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00012964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR30263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="233" mass="27255" checksum="366A00A651593CC2" modified="2009-07-28" version="1">MNKFIGSTVFNRLLTRNTRMTAPVWKPQDHVPQEVKDLLQSSGDNHVLAFLHIVQLLKVQRRTGWLDHNISPCESIADHMYRMGITAMLIRDPKINRDKCVRIALVHDIAEALVGDITPLDPVGKEEKHRREWETIQYLCEDLVKPYNAQAAQEIMDDWLAYENIASPEARYVKDIDKFEMLVQCFEYEKLHNGKRDLDQFWGAMSSIKTEEVKGWAQDLLQKREQFYNSLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2Q5</accession>
    <name>C5E2Q5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0H06864p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0H06864g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556178.1">
        <property type="nucleotide sequence ID" value="XM_002556132.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2Q5"/>
    <dbReference type="FunCoup" id="C5E2Q5">
        <property type="interactions" value="98"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2Q5"/>
    <dbReference type="GeneID" id="8294494"/>
    <dbReference type="KEGG" id="lth:KLTH0H06864g"/>
    <dbReference type="eggNOG" id="ENOG502S0ZV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068947_0_0_1"/>
    <dbReference type="InParanoid" id="C5E2Q5"/>
    <dbReference type="OMA" id="CYQMKQI"/>
    <dbReference type="OrthoDB" id="4067487at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048743">
        <property type="entry name" value="AME1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20994">
        <property type="entry name" value="CENPU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inner kinetochore subunit AME1" evidence="2">
        <location>
            <begin position="160"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR30316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="352" mass="37957" checksum="70CB17D068701A94" modified="2009-07-28" version="1">MSTKRISPFHKRARSGAMLMDRDVKLLYRQRGSALRRTGQSIVISQAKRPKHSGRAVRERERDVAAAPGPAVGEQPSSPPPLISDGFLGSPAHELTREPTQLPHSPPAPLSPSPLVPLPALDADLDADLGAGLDEDLNADLGASPGVAGHAEAAPLLPAPQFSFSDVPLSRLHSNTVSLSSIEVVASLINSLFEEQLVPQCFAEFDTAATPEDRLMHKLDIKLYSTFVQAVLADLRDLLDINVSNNELLTQLKQTIKRKSALTQTLLDVRHETADWEANAGADEQLEELRQKSAVNAKLQALAQDVRTASADASAPPDADHALDDLTAVLDPYNGVLAQLQTFNQSLEKLLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2T1</accession>
    <name>C5E2T1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0H07480p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0H07480g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556204.1">
        <property type="nucleotide sequence ID" value="XM_002556158.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2T1"/>
    <dbReference type="FunCoup" id="C5E2T1">
        <property type="interactions" value="48"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2T1"/>
    <dbReference type="GeneID" id="8294522"/>
    <dbReference type="KEGG" id="lth:KLTH0H07480g"/>
    <dbReference type="eggNOG" id="ENOG502QTTD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054089_0_0_1"/>
    <dbReference type="InParanoid" id="C5E2T1"/>
    <dbReference type="OMA" id="IMRNEFK"/>
    <dbReference type="OrthoDB" id="3996692at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="150"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="217"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="323"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="264"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="355"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="158"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="226"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="326"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR30342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="395" mass="44342" checksum="FA9B6FB75629B20B" modified="2009-07-28" version="1">MGGLKLLRAANPKLFSDPECDKYPTSYDELSVFLKREQVYSGAELATELDYLDSVLYGDRERGIDLDRKLRVEYAMFKATTMQNERAGPGPSLDELVVLNEQIQNAHKQRTSEITQKQNAFYAALMALLQSVSLDQAALQSILTAAEGGRDCDDLEKSSSGMSRSIQPSPLDEVTSYLISGALQHGVELKPSTLEPESPRDDITFFKECIDSLLRNVGLTQPPAENQDRDNDRKDETGDSDVAVALKDLQLAHSFLTKKFEGDRNEYLHSIDKLTRTNKELSHELLTYHSELTTVKENCDALTKEREELKSQLEYHTSLNTPTLVASSPVSNASASSAASGSKSYSIGVMRSEFKKVLTDTQNRYEKELQEERELRKQLEQELSSFRTRRSGHNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2Y8</accession>
    <name>C5E2Y8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0H08910p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0H08910g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TACO1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556261.1">
        <property type="nucleotide sequence ID" value="XM_002556215.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2Y8"/>
    <dbReference type="FunCoup" id="C5E2Y8">
        <property type="interactions" value="333"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2Y8"/>
    <dbReference type="GeneID" id="8294586"/>
    <dbReference type="KEGG" id="lth:KLTH0H08910g"/>
    <dbReference type="eggNOG" id="KOG2972">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062974_1_0_1"/>
    <dbReference type="InParanoid" id="C5E2Y8"/>
    <dbReference type="OMA" id="FGPGGCM"/>
    <dbReference type="OrthoDB" id="2017544at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.200:FF:000002">
        <property type="entry name" value="Probable transcriptional regulatory protein CLM62_37755"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.980">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00693">
        <property type="entry name" value="Transcrip_reg_TACO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017856">
        <property type="entry name" value="Integrase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048300">
        <property type="entry name" value="TACO1_YebC-like_2nd/3rd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049083">
        <property type="entry name" value="TACO1_YebC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002876">
        <property type="entry name" value="Transcrip_reg_TACO1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026564">
        <property type="entry name" value="Transcrip_reg_TACO1-like_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029072">
        <property type="entry name" value="YebC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12532">
        <property type="entry name" value="TRANSLATIONAL ACTIVATOR OF CYTOCHROME C OXIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12532:SF0">
        <property type="entry name" value="TRANSLATIONAL ACTIVATOR OF CYTOCHROME C OXIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20772">
        <property type="entry name" value="TACO1_YebC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01709">
        <property type="entry name" value="Transcrip_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75625">
        <property type="entry name" value="YebC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TACO1/YebC-like N-terminal" evidence="4">
        <location>
            <begin position="28"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="TACO1/YebC-like second and third" evidence="3">
        <location>
            <begin position="111"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF20772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR30399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="286" mass="31123" checksum="66596050CE065965" modified="2009-07-28" version="1">MLRLLKPGRCPSALRLFSTCSARLSGHNKWSTIKHDKAKNDAEKNKLFNKFANQIALAVKLGGGSTDPALNIRLAAAIESANKNNVTKRVVENAIKKGSGAGGKDGSKSGSCVYEGMGPGGVAFVVEALTDNKNRTIGLVRSTFSKVNGSMTPTLYFFDRKGYIIVQPPQELDDFDQVFEKVLEHEGVEDLEQVDPENDALAAEDLAPGFASSVYELSTEPAATNQVASSLKQHGFHIKEVGIGYVPKSDMLVDVQNEDTMKSVEKFMGQLDEIDDVTDFYCNLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5E374</accession>
    <name>C5E374_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0H10934p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0H10934g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PAF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556347.1">
        <property type="nucleotide sequence ID" value="XM_002556301.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E374"/>
    <dbReference type="FunCoup" id="C5E374">
        <property type="interactions" value="373"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E374"/>
    <dbReference type="GeneID" id="8294678"/>
    <dbReference type="KEGG" id="lth:KLTH0H10934g"/>
    <dbReference type="eggNOG" id="KOG2478">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021991_3_1_1"/>
    <dbReference type="InParanoid" id="C5E374"/>
    <dbReference type="OMA" id="LVCRIKY"/>
    <dbReference type="OrthoDB" id="10260285at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016593">
        <property type="term" value="C:Cdc73/Paf1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007133">
        <property type="entry name" value="RNA_pol_II-assoc_Paf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23188">
        <property type="entry name" value="RNA POLYMERASE II-ASSOCIATED FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23188:SF12">
        <property type="entry name" value="RNA POLYMERASE II-ASSOCIATED FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03985">
        <property type="entry name" value="Paf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="122"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="362"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="364"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="383"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR30485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="401" mass="46358" checksum="5228E4C5F6DA7509" modified="2009-07-28" version="1">MSKKHEYIAKIRYRNDLPAPLLPPKLLKYQGPPNEEVDSPQLITSLYAKSNVTPLIKLNDDLSMPLDLTKIPGVLDHKDTKLLHGFENIKLHPEDRVLLRDPRMDRLTKTDTSKVTFLRRTEYVSSSSAPKPSNRKRPVSEAIDPDDEILTPAQRVKRVESTFDSVTDDLSKVKHPFKKNVHAVKAWELLPDTVSMDQTFFTIRLVGSAALDKAEKEQLALSTALFRPVELEEDEWVSLYTTEKNSSEEYEKNLEQQIDEITDDDRVYKFKRLRDFDMKQLQPAGSGLFSELALRFNNDKKTIYYTPLRSRIELRRRRVNDVIKPLVRENNLDQINIKIKNPTTQETRIRDKIRMKFDPIDFPNIEDEIDDEAPQEEQVTKVSEAASQDAQQGSQDVDAEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3J1</accession>
    <name>C5E3J1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0H13992p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0H13992g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556464.1">
        <property type="nucleotide sequence ID" value="XM_002556418.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3J1"/>
    <dbReference type="STRING" id="559295.C5E3J1"/>
    <dbReference type="GeneID" id="8294826"/>
    <dbReference type="KEGG" id="lth:KLTH0H13992g"/>
    <dbReference type="eggNOG" id="KOG0725">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_1_1"/>
    <dbReference type="InParanoid" id="C5E3J1"/>
    <dbReference type="OMA" id="IENFTKW"/>
    <dbReference type="OrthoDB" id="5325318at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760:SF115">
        <property type="entry name" value="3-OXOACYL-[ACYL-CARRIER-PROTEIN] REDUCTASE FABG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASES/REDUCTASES FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR30602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="315" mass="34041" checksum="9EC06CC1D87634C3" modified="2009-07-28" version="1">MTNSNSNGMVESAQQDLPSMPKLQDILPSFRLDGHVTIVTGGSGGLAHTLTQALLAQGSDVALLDLSVERLEYTQRELQEFARANNLNKVTISAWPCDIGDAAIVEEVVRAIPLKHNGIVPDKLIHTAGYCQNIPAMDYDARQAEAVVRVNLMGSLYICQSMSRQLLQKSQRRKSVSSDSSEAGEAVPQFPHASYVLIGSMSGCIVNNPQPQVAYNMSKAGVIHMARSLACEWARYGIRVNSISPGYIATALTKKVISGSPEGAALQQEWTSRVPMGRMADPREFVGAMLYLLSSSASSYTTGENLVVDGGYTCW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3Q0</accession>
    <name>C5E3Q0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0H15400p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0H15400g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556523.1">
        <property type="nucleotide sequence ID" value="XM_002556477.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3Q0"/>
    <dbReference type="FunCoup" id="C5E3Q0">
        <property type="interactions" value="149"/>
    </dbReference>
    <dbReference type="GeneID" id="8294889"/>
    <dbReference type="KEGG" id="lth:KLTH0H15400g"/>
    <dbReference type="eggNOG" id="ENOG502RY6S">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047747_0_0_1"/>
    <dbReference type="InParanoid" id="C5E3Q0"/>
    <dbReference type="OMA" id="HIETYPI"/>
    <dbReference type="OrthoDB" id="376826at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17316">
        <property type="entry name" value="Perilipin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR30661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="271" mass="29894" checksum="9AD6C7E439ADB85B" modified="2009-07-28" version="1">MSESVESSKSFERAKTFPLLQQTHEFLTQLPACRVLEANAKPILSSIASSKPAKWMLPAAKMADNIADKGLELAESWAPSLKTKTYQDLRHDALVPFNYSKETVGKAANATLGAADTYVYEPAHAHMSAFRQFYNEKVYDTNGRPLVRGSIDPLVGPCNQRLEAVTKAYFPDGEEVPSNGYSTEVSRTLALSVNFVQRMAPWANRKVTDLALAPYNYASHVNAVFNGNLDKQESLSLANSWTATREALCTLNEEALQYVKPKGKNSNIVAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3Q6</accession>
    <name>C5E3Q6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0H15532p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0H15532g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Mitochondrion intermembrane space</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cytochrome c oxidase subunit 6B family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556529.1">
        <property type="nucleotide sequence ID" value="XM_002556483.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3Q6"/>
    <dbReference type="FunCoup" id="C5E3Q6">
        <property type="interactions" value="182"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3Q6"/>
    <dbReference type="GeneID" id="8294895"/>
    <dbReference type="KEGG" id="lth:KLTH0H15532g"/>
    <dbReference type="eggNOG" id="ENOG502S7HF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_142408_0_0_1"/>
    <dbReference type="InParanoid" id="C5E3Q6"/>
    <dbReference type="OMA" id="NCARSWV"/>
    <dbReference type="OrthoDB" id="5545577at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.140:FF:000003">
        <property type="entry name" value="Cytochrome c oxidase assembly factor 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.140">
        <property type="entry name" value="Cytochrome c oxidase, subunit VIb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048281">
        <property type="entry name" value="COA6_fun"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048280">
        <property type="entry name" value="COX6B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036549">
        <property type="entry name" value="CX6/COA6-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47677">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47677:SF1">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02297">
        <property type="entry name" value="COX6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47694">
        <property type="entry name" value="Cytochrome c oxidase subunit h"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR30667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="109" mass="12946" checksum="A38A4E7F2C870E8F" modified="2009-07-28" version="1">MGWFTKAKPIDDQSRPPSRSSRKQCWESRDIFFQCLDKISVVNALDPKNQDAIQKNCKAQENKYEQDCATSWIKYFKEKRVVDHKREKFMQEMESKNAQQIDLSGVVRR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-05-31" modified="2025-06-18" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3S7</accession>
    <name>NUR1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName>Nuclear rim protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">NUR1</name>
        <name type="ordered locus">KLTH0H15994g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Member of a perinuclear network that controls recombination at multiple loci to maintain genome stability. Required for rDNA repeat stability.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the NUR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556550.1">
        <property type="nucleotide sequence ID" value="XM_002556504.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3S7"/>
    <dbReference type="FunCoup" id="C5E3S7">
        <property type="interactions" value="40"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3S7"/>
    <dbReference type="GeneID" id="8294916"/>
    <dbReference type="KEGG" id="lth:KLTH0H15994g"/>
    <dbReference type="eggNOG" id="ENOG502S7S0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033252_0_0_1"/>
    <dbReference type="InParanoid" id="C5E3S7"/>
    <dbReference type="OMA" id="TRSHIFQ"/>
    <dbReference type="OrthoDB" id="3363151at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043007">
        <property type="term" value="P:maintenance of rDNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007096">
        <property type="term" value="P:regulation of exit from mitosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018819">
        <property type="entry name" value="Nur1/Mug154"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28293">
        <property type="entry name" value="NUCLEAR RIM PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28293:SF1">
        <property type="entry name" value="NUCLEAR RIM PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10332">
        <property type="entry name" value="DUF2418"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0539">Nucleus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000409030" description="Nuclear rim protein 1">
        <location>
            <begin position="1"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="151"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="255"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="547"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="560"/>
            <end position="571"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="592" mass="67038" checksum="A46F47D3BEC9615E" modified="2009-07-28" version="1">MTFRLSRFESPLIEDDGESRASLLGYSPENELYTDVDENRESRFRRFMSFISSSPYDMFLAINEHVESIDWDSKASTIAGPLGNFFTCSLYTARLLQDSLIRPNQQKLDKKRDSFDLSRSEILRKFEYLSQVPKSGVVVTHLNWYWKFLTFLNVALQITVGFLILINLFVAYKFLIGHFQVYSLFYTKTSPRSKNVTKRSLSDLSFKSLEEVTNSSLWTMIRYMFVRKRLIIKDAPKGKYYYQLRKWTPGKFYTALFSAFSPISVIFLLVTEVSFKTALAVIGHQYILFLVLFKRYESRLDDEACLAKAHFEEINEKVIKPKTTIKTQDAMVDATTYGGGAAFFPSFTTTRSHIFQTHAVTGDIITERYNPETRNFEDVENTGRAKNYISQIQGVSHGQQVVSRSKAMNGATARPQFFSRQPSPSKIGTPSIILNYRTSPFSAPTTPTLKPVNGVQNGQSIFRNSPDPSKANSLNCDTSHLSRNNTLSRLRRNSVSPTKSGNYCSASGMRAIHKSNFGADSSVSYSMEAPSNELPFEEVARRGRHPFEITASRDLPAGRSSAVSSRHSSISPFKGNTSFAGRESLDSRPPFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-04-02" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBB0</accession>
    <name>C5DBB0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0A01056p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0A01056g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551509.1">
        <property type="nucleotide sequence ID" value="XM_002551463.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBB0"/>
    <dbReference type="GeneID" id="8290300"/>
    <dbReference type="KEGG" id="lth:KLTH0A01056g"/>
    <dbReference type="eggNOG" id="ENOG502S4ZF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_411154_0_0_1"/>
    <dbReference type="InParanoid" id="C5DBB0"/>
    <dbReference type="OMA" id="SAYNYEM"/>
    <dbReference type="OrthoDB" id="4069534at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="140"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="711"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="245"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="316"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="331"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="345"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="376"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="414"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="444"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="473"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="492"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="512"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="634"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="739"/>
            <end position="756"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="756" mass="82622" checksum="AB17B3EE6871FBE0" modified="2009-07-28" version="1">MEREASSLRDPELEDYLARVERLDSARNGSRRSPDAHDQRPKYRPEDLERAQRLLDGDYTGYDSGTSPTKYSGSVPQIAYRSAYNYERTFSPKRGAGSPRRSDAGSAPALGLDSSPDGTRRYTVSEQDYLLLQKLKREAVGDPLVSALPSRGRPRENRVVRTVTLADDGDDNDEIPPPLPTRRAASPVLPPPDREEDSTPTKPARPLGLSVPSKSVAPAKPARVPDLSMRLKSAAPASPVVETGTKPSTPLQKNTAVSIDNRDSLVPKKPPPRKPASLTEASKADGFIKILSDRAKKPVPPKPETLKKSASYLESLENNKLTTASLDKPSKPSIQSKPSIQSKPTSYLDSLQKNKVTVSTPVREPKSPSPIAKPQHTPQMFMNSVLKTESSRPLQDSSAQSPKPFVIPPKRKSPQKEPVPESDEKAEVSFLSLRSSLKPAQSFKKSDAREPGFRSDEQKLVIPKLRRPKVQDDSAQFDGQESLKSVPRSIEGNGSASETASQRQRLNVAPPKPERKVSTPEALSKRESLAKAPPKPARKISTPEALSKRESLNKAPPKPPRKVSALEALSKRESLSKAPPKPARKVSTPEAVNKRESLSKPPPKPSRKVSMPEAMKRLEAIKAKKLSQFEGTEVSEKPPQKLEPTVESKGSPLKAKTEANNQKAPESELAALIAAKQLANKGKSRTAPELSNRAHLPQAIPFMAQLDNDKIAKMLRPASETDISRPRASDGDNQLNHPTKSRARGPKRKLPKGVKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBF5</accession>
    <name>C5DBF5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Vacuolar-sorting protein SNF8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0A02200g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the endosomal sorting complex required for transport II (ESCRT-II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the endosomal sorting complex required for transport II (ESCRT-II).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the SNF8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551554.1">
        <property type="nucleotide sequence ID" value="XM_002551508.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBF5"/>
    <dbReference type="FunCoup" id="C5DBF5">
        <property type="interactions" value="691"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBF5"/>
    <dbReference type="GeneID" id="8290351"/>
    <dbReference type="KEGG" id="lth:KLTH0A02200g"/>
    <dbReference type="eggNOG" id="KOG3341">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070147_0_1_1"/>
    <dbReference type="InParanoid" id="C5DBF5"/>
    <dbReference type="OMA" id="QIVEVCM"/>
    <dbReference type="OrthoDB" id="283883at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000814">
        <property type="term" value="C:ESCRT II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000397">
        <property type="entry name" value="Vacuolar-sorting protein SNF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016689">
        <property type="entry name" value="ESCRT-2_cplx_Snf8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040608">
        <property type="entry name" value="Snf8/Vps36"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12806">
        <property type="entry name" value="EAP30 SUBUNIT OF ELL COMPLEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12806:SF0">
        <property type="entry name" value="VACUOLAR-SORTING PROTEIN SNF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04157">
        <property type="entry name" value="EAP30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017215">
        <property type="entry name" value="ESCRT2_Vps22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR017215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR21112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="234" mass="27176" checksum="7FC72D5ACC6F0EED" modified="2009-07-28" version="1">MKHGLAAFNSDQHNRYSTTASILSKQDRELDEQLNVFQQRLVEFAKVHNKELRESPEFRMKFIRMCASIGVDPISLFDKERHLFDVNDFFYEICVKIIEICRKTKNLNGGVISFDELHKGYFEQFKVQLNDLQKAVEMLETLDGGFEVFCIRGKKFLRSVPNELTNDQARILEVCSILGYASISLLRLNLEWEPVRSDAILKEMVANGLLWIDSQAQGETLYWDPAWIVRSNFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBL5</accession>
    <name>C5DBL5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0A03586p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0A03586g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551614.1">
        <property type="nucleotide sequence ID" value="XM_002551568.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBL5"/>
    <dbReference type="FunCoup" id="C5DBL5">
        <property type="interactions" value="2219"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBL5"/>
    <dbReference type="GeneID" id="8290414"/>
    <dbReference type="KEGG" id="lth:KLTH0A03586g"/>
    <dbReference type="eggNOG" id="KOG1414">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056594_0_0_1"/>
    <dbReference type="InParanoid" id="C5DBL5"/>
    <dbReference type="OMA" id="NQAYINM"/>
    <dbReference type="OrthoDB" id="295274at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14687">
        <property type="entry name" value="bZIP_ATF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000053">
        <property type="entry name" value="BZIP transcription factor AtfA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051027">
        <property type="entry name" value="bZIP_transcription_factors"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19304">
        <property type="entry name" value="CYCLIC-AMP RESPONSE ELEMENT BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="9">
        <location>
            <begin position="321"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="163"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="222"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="339"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="189"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="231"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="272"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR21172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="424" mass="46150" checksum="53187A565C06084A" modified="2009-07-28" version="1">MERVHIKTEESRAGLPPAHAQGVLVGGAMNHGQVGANYGAPAGGGMGFGGLGSPGTLGMHGASGTPGAAETGNLREAGGVFPHLSKYQLNNSGGMADSSLQECLSPFFQPFGVDVSHFPLTNPPIFQSSLTNFSDHPRRRRISISNGQISQLGDATHTFDDLYYTQPPPMPIRHERQDAQPHIGESKSVPPQGAKAAPAPVQVQGAPTAQHFLPQPAAADVVPGQQHGQLQVPPQHAQHPQHPQHTQPVQHAQQPMDGPHQHAPVPDELYSKDSAFSSSFSTPTDASLLVPENSRPPVQALPKEYQVRPRTTYEDGPGTPEWKRARLLERNRIAASKCRQRKKIAQQQLQKDVSQLTQENRFMRKKLDYYEKLVNKFKKFTEIHMASCGGSEQLSVIEDLLKIDHNIDDKQGGSSQSTEEEARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBN6</accession>
    <name>C5DBN6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0A04114p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0A04114g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
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        <property type="entry name" value="UBIQUITIN LIGASE SPECIFICITY FACTOR/HREP PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="UBIQUITIN-BINDING SDF UBIQUITIN LIGASE COMPLEX SUBUNIT"/>
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    </dbReference>
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        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
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        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="1 4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="F-box" evidence="6">
        <location>
            <begin position="219"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="344"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="384"/>
            <end position="423"/>
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    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="424"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="463"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="563"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="603"/>
            <end position="625"/>
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    <feature type="region of interest" description="Disordered" evidence="5">
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            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="96"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="532"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="103"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAR21193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="658" mass="74632" checksum="5EEF1AEDA49843AD" modified="2009-07-28" version="1">MSQSNHSASNKTTITVEDKHIGTCIKGIKKLESLPWRRRRKLTVMLEEFPNQMGALIKPEVRSTQSTNMHAKEEAPDAANSSVVASGRLNGKRGLEEYEEDQPSTAEVSSEASAQNVNKRRRGLPQYNFLRFCYRHNPDIQQSPTHTACRKQDQQRHSLLQRKIEKLEPEEQRQVQNVLSTFTSCNDKMRKLLLEGMLTNCCFPQLSFVASEVQNMIKIDFISTLPEEVSLKILGYLDCESLCNAARVCRRWKQLADDDKVWYHMCQQHIDRKCPNCGWGLPLLHMKRARFIDSTEQKSDPKDACATAAASGGHRKTRPWKVIYRERFKVESNWRKGAAHIQDFKGHMDGVLTLQFNYRLLFTGSYDSTVAIWDLATGKLVRRLTGHRDGVKTLYFDDQKLITGSLDRTIRVWNYVTGACVSTYRGHSDSVLSVDSYKKIIVSGSADKTVKMWHVESRTCYTLRGHSEWVGCVKLHPKSFTCFSGSDDTTIRMWDIRSNTCLKVFRGHVGQVQKVLPLNILDTENLVTDPASEAQRNDEPAANDPSEGQLAPLDDSLPYPSHLLSCSLDNTIKLWDVRTGKCVRTHFGHLEGIWDIAADNFRIISGAHDKTVKVWDLQSGKCIHTFDSHHAPITCVGIGDSEFVSGDELGCVKLHRFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBN9</accession>
    <name>C5DBN9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="19">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ordered locus">KLTH0A04180g</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66132"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:66133"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 18">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endosome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="19">Membrane</location>
            <topology evidence="19">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 19">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="19"/>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551638.1">
        <property type="nucleotide sequence ID" value="XM_002551592.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBN9"/>
    <dbReference type="FunCoup" id="C5DBN9">
        <property type="interactions" value="401"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBN9"/>
    <dbReference type="GeneID" id="8290441"/>
    <dbReference type="KEGG" id="lth:KLTH0A04180g"/>
    <dbReference type="eggNOG" id="KOG0210">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000846_3_1_1"/>
    <dbReference type="InParanoid" id="C5DBN9"/>
    <dbReference type="OMA" id="IAITTWH"/>
    <dbReference type="OrthoDB" id="377733at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140346">
        <property type="term" value="F:phosphatidylserine flippase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000085">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000009">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF5">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 17 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="9 18 19" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 17 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 19" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="205"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="400"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="427"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="917"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="949"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="999"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1032"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1059"/>
            <end position="1077"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1089"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="21">
        <location>
            <begin position="150"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="22">
        <location>
            <begin position="887"/>
            <end position="1117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="62"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="62"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="85"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="16">
        <location>
            <position position="486"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="486"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="486"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
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        <location>
            <position position="487"/>
        </location>
        <ligand>
            <name>ATP</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="488"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="488"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
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        <location>
            <position position="575"/>
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        <ligand>
            <name>ATP</name>
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        </ligand>
    </feature>
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        <location>
            <position position="618"/>
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        <ligand>
            <name>ATP</name>
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        </ligand>
    </feature>
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        <location>
            <position position="642"/>
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        <ligand>
            <name>ATP</name>
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        </ligand>
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        <location>
            <position position="671"/>
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        <ligand>
            <name>ATP</name>
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        <location>
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        <ligand>
            <name>ATP</name>
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        <location>
            <position position="754"/>
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        <ligand>
            <name>ATP</name>
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        </ligand>
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        <location>
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            <name>ATP</name>
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        <location>
            <position position="840"/>
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        <ligand>
            <name>ATP</name>
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        </ligand>
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        <location>
            <position position="860"/>
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        <location>
            <position position="863"/>
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            <name>ATP</name>
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        <location>
            <position position="864"/>
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            <name>ATP</name>
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        <location>
            <position position="864"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004337"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00008109"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
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            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00034036"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00049128"/>
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        <source>
            <dbReference type="PIRSR" id="PIRSR606539-1"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU362033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF16209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF16212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="CAR21196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1130" mass="126131" checksum="8D4C711BA4BED673" modified="2009-07-28" version="1">MSFTPPPGSNKEPAAPANRHNSFDLLDPQFEDSLDAALESLQIHSEGAPTGEDFEMKSIASNDNHNAGLHSDTQPLINDGLGPNDSWSRGSRNSHSAEHRQPFWSRITGIVRSSSTPRSSKYKSTGKSIQLTDQQLEREIHPGTTPIYDKYKYAANAVSNAKYNPVTFIPVILYEQFKFFFNLYFLLVALSQAIPALRIGYLSSYIVPLAFVLIVTMSKEALDDIQRRRRDSEANNELYEVLSKSQLVPSKDLKVGDLIKIGKGARAPADLILLQSSEPSGEIFIKTDQLDGETDWKLRIASPLTQHLSQEDLLHKINITASVPEKSIHNFLGKLTFNDSQSQPLSVDNTMWANTVFASTGTAVACVVYTGSDTRQAMNTTMSSVKTGLLELEINSLSKILCVCVFILSVALVAFAGFNNSDWYVDIMRYLILFSTIIPVSLRVNLDLGKSVYAYQIEHDATIPDTIVRTSTIPEDLGRIEYLLTDKTGTLTQNDMELKKLHLGTVSYTMDTMDIVTDYVRGMTSGADSTVLPSASKKDLPSRVRDLVLALALCHQVTPTFEDGELTYQAASPDEIAIVKFTESVGLTLFKRDRHSITLLHDQSGSYFDFDIVQVFPFNSDNKRMGIIIFDKQKQEHWFFQKGADVVMSKIVQTNDWLEEETGNLAREGLRTLVIGRKKLSQKVFDNFTKEYKDASLTMLNREALMSNVVKKHLEHNLEILGLTGVEDKLQHDAKSSIELLRNAGIKIWMLTGDKVETARCVSISAKLVSRGQYVHTVTKIGKPEGALGQLELLKINQNSCLLIDGESLATYLEYYRQEFFEIVVHLPAVIACRCTPQQKADVATFIREATGKRVCCIGDGGNDVSMIQAADVGVGIVGKEGKQASLAADFSITQFCHLSKLLLWHGRNSYKRSAKLAQFVIHRGLLISVCQAVYSISSKFEPIALYQGWLMVGYATCYTMAPVFSLTLDHDIDESLTKTYPELYKELTAGKSLSYKTFFVWVALSVFQGCVIQGFSQAFTSLDQLDFKRLVALSFTVLIINELIMVAIEIYTWNKTMIICEILTFLVFVGSIPLLGDYFDLHFMTQPNFYAKLALILGLSVVPVWTAKALHRKLNPPSYAKVQQFYFNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBW0</accession>
    <name>C5DBW0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0A05764p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0A05764g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551709.1">
        <property type="nucleotide sequence ID" value="XM_002551663.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBW0"/>
    <dbReference type="FunCoup" id="C5DBW0">
        <property type="interactions" value="96"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBW0"/>
    <dbReference type="GeneID" id="8290513"/>
    <dbReference type="KEGG" id="lth:KLTH0A05764g"/>
    <dbReference type="HOGENOM" id="CLU_1378348_0_0_1"/>
    <dbReference type="InParanoid" id="C5DBW0"/>
    <dbReference type="OMA" id="QFAYYKY"/>
    <dbReference type="OrthoDB" id="4061338at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="127"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="198" mass="21356" checksum="B93EC29BB520905C" modified="2009-07-28" version="1">MPAELERKPLGAVSQSRINQLKGRTHGKVGKPAATTKLPSIMSIIHQETSADTHAPETNTRGSELARPSKSAPECDFDLISEKLRIRMQLAHYKFKTKQGHLKFRQLQPAVPAVTLSAPAEGPRDASSGTTSPLSSSQRRSKPSRRLVGSQGALRTPVKPRTLSSHALAPRAAAESGQNTPMSVEAARSLIDLFTSQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>C5DC26</accession>
    <name>C5DC26_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">General transcriptional corepressor TUP1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Transcriptional repressor TUP1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0A07282g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the WD repeat TUP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551775.1">
        <property type="nucleotide sequence ID" value="XM_002551729.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DC26"/>
    <dbReference type="FunCoup" id="C5DC26">
        <property type="interactions" value="2167"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DC26"/>
    <dbReference type="GeneID" id="8290582"/>
    <dbReference type="KEGG" id="lth:KLTH0A07282g"/>
    <dbReference type="eggNOG" id="KOG0266">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_57_23_1"/>
    <dbReference type="InParanoid" id="C5DC26"/>
    <dbReference type="OMA" id="HYLVPYN"/>
    <dbReference type="OrthoDB" id="17410at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000111">
        <property type="entry name" value="Transcriptional repressor rco-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000043">
        <property type="entry name" value="Transcriptional repressor TUP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013890">
        <property type="entry name" value="Tscrpt_rep_Tup1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848:SF8">
        <property type="entry name" value="F-BOX AND WD REPEAT DOMAIN CONTAINING 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08581">
        <property type="entry name" value="Tup_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0678">Repressor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2 9" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Transcriptional repressor Tup1 N-terminal" evidence="11">
        <location>
            <begin position="12"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="451"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="486"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="527"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="577"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="631"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="159"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="222"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="415"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="167"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="244"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00060760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00073268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00074105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR21333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="725" mass="78322" checksum="DB98EA6251B92857" modified="2009-07-28" version="1">MASSAVSASQNKLNELLEAIRQEFGQISQEANTYRLQNQKDYDFKINQQLAEMQQIRNTVYELELTHRKMKDAYEEEIGRLKLELDQRDRQVATLAAAAQQQQHQQQHQQQQQQHQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQPTGPILASSNAATPGIQNPVLGSQAVQSPALSAAGPAAPAQAPRLAALEPSQTAPTITAQAPHMKKLASEHQINSPPVQGAGIPTGPAVLAAPAAPGTPGTPAAALAAAAAPTPPSASGGPLAAPTNQGAVAPPAPMAPMAPIAAPVSENKSQEYYLVPPHQRASHAKPIPPFLLDLDSQLVPQHLKKQTNDYYILYNPALSRELDVDLHVSLDHSSVVCCVRFSNDGEYLATGCNKTTQVYKVSTGELVARLSEDAASAPSGNSGDGASGAENAEQTPAVSTSASSDLYIRSVCFSPDGKFLATGAEDKLIRIWDLTTRRIVMTLQGHEQDIYSLDYFPSGDKLVSGSGDRTVRIWDLRAGQCSLTLSIEDGVTTVAVSPGDGKLIAAGSLDRTVRIWDSETGFLVERLDSENELGTGHKDSVYSVVFTRDGHGVVSGSLDRSVKLWNLRSANGGSTEGKANTAASEVTYTGHKDFVLSVATTQNDEFILSGSKDRGVLFWDTPSGNPLLMLQGHRNSVISVAVANDHPLGPEYGVFATGSGDCKARIWKYTKKSEPKLREVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DC74</accession>
    <name>C5DC74_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0B00572p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0B00572g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551823.1">
        <property type="nucleotide sequence ID" value="XM_002551777.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DC74"/>
    <dbReference type="GeneID" id="8290642"/>
    <dbReference type="KEGG" id="lth:KLTH0B00572g"/>
    <dbReference type="eggNOG" id="KOG2533">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_0_5_1"/>
    <dbReference type="InParanoid" id="C5DC74"/>
    <dbReference type="OMA" id="PILWITC"/>
    <dbReference type="OrthoDB" id="3639251at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033229">
        <property type="term" value="F:cysteine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF63">
        <property type="entry name" value="HIGH AFFINITY CYSTEINE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="76"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="234"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="334"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="371"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="397"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="431"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="464"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR21385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="538" mass="60316" checksum="27B21C72D8633E53" modified="2009-07-28" version="1">MLATESDRSSGSLSLENADKILVNIVENEIGQSEDQIEDKVTTSRDADVTLGFLLDNNVLVPAVSYEEERRLARKVLWTIVPLCALIDFVLYADKATASYTSIFGMWKDTNLDQNKYSNSTTLFYVGFIIGQVNLLFLQKFPVGRVVAALSFFWSLIIYLHTVTTSYQGIYALRFFLGFVEAIAVPALNVTMGQFLTAEEKSWTAPLFYTTCLGNEIVVGFIAFGILHAKPAFAIWKLFMIVIATISLLVTILVLFLYPNNPTDARFLNLKEKIWVIRRVQRTTGSSIEQKVIKKQQIIEALKDPVSWLFCAFFFCNQLSNNLVYQEKLLFKEIGISTLGSTLVSVAYGSFSVTCAVIASSIIRKKKNSTAYSVVFWTLFPLVGSIAMVTLPWDRTYALLAMICICAPKGIAWILMFSWTSTSTSGHTKKITRNALIMFWYGVANIIAPQLWRANDGPRYYPAWIVQIVFAFFLAPISALAIRYILARRNEERKSSLDGGTPLGLIKSTDIESASTIKGNIALLDLTDLENDHFIYPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>C5DC87</accession>
    <name>C5DC87_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">KLTH0B01034p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0B01034g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 32 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551836.1">
        <property type="nucleotide sequence ID" value="XM_002551790.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DC87"/>
    <dbReference type="FunCoup" id="C5DC87">
        <property type="interactions" value="121"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DC87"/>
    <dbReference type="GeneID" id="8291090"/>
    <dbReference type="KEGG" id="lth:KLTH0B01034g"/>
    <dbReference type="eggNOG" id="ENOG502QW2I">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022381_5_0_1"/>
    <dbReference type="InParanoid" id="C5DC87"/>
    <dbReference type="OMA" id="WIPENVS"/>
    <dbReference type="OrthoDB" id="411251at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000136">
        <property type="term" value="C:mannan polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000009">
        <property type="term" value="F:alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.20:FF:000002">
        <property type="entry name" value="Initiation-specific alpha-1,6-mannosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007577">
        <property type="entry name" value="GlycoTrfase_DXD_sugar-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039367">
        <property type="entry name" value="Och1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31834:SF11">
        <property type="entry name" value="GLYCOSYLTRANSFERASE HOC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31834">
        <property type="entry name" value="INITIATION-SPECIFIC ALPHA-1,6-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04488">
        <property type="entry name" value="Gly_transf_sug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="51"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="109"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR21398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="434" mass="49860" checksum="09D590E9CCD25388" modified="2009-07-28" version="1">MRLYHIFRFTLRNNSSNHLDADQSFNSTIKIFPTQRSANMPKSRRPTWKRYVVWAVLLTIALALVGRFVSNRTATDVQAILQGLPKDIAPGADAASAQKQDVNMVELFEKLSQDLLAKQEDQIKKFEKERKILEKKIQDLKQPATHATLREKLAHMFEYGTSKKFPAFIWQTWPYSDLDDRMDPTLQSFERRWGDKNPGFVHEIINDDTASALIHYMYASIPEVIEAYDALPSVYLKADFFKYLILFARGGVYADIDTDPLQPVPNWIPENVSPREIGLIIGIENDANSPDWRSNYVRRLQFGNWIMQAKPGHPVMREIVAKVTEETLKRRADGELRMNLRNDLNIMSWTGSGIWTDVVFTYFNDYVQSGILSKITWKEFHNLGVPKLVGDVLVFPQFSFDAPEKEEDGNKALQFCRHTGLKSWKAAPKVAGDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCX3</accession>
    <name>C5DCX3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ribosome assembly protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Elongation factor-like 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0B06512g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552072.1">
        <property type="nucleotide sequence ID" value="XM_002552026.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCX3"/>
    <dbReference type="SMR" id="C5DCX3"/>
    <dbReference type="FunCoup" id="C5DCX3">
        <property type="interactions" value="853"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCX3"/>
    <dbReference type="GeneID" id="8290911"/>
    <dbReference type="KEGG" id="lth:KLTH0B06512g"/>
    <dbReference type="eggNOG" id="KOG0467">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002794_3_1_1"/>
    <dbReference type="InParanoid" id="C5DCX3"/>
    <dbReference type="OMA" id="FARCDIQ"/>
    <dbReference type="OrthoDB" id="364892at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:cytosolic ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01681">
        <property type="entry name" value="aeEF2_snRNP_like_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04096">
        <property type="entry name" value="eEF2_snRNP_like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01885">
        <property type="entry name" value="EF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16268">
        <property type="entry name" value="EF2_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16261">
        <property type="entry name" value="EF2_snRNP_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.240:FF:000006">
        <property type="entry name" value="Elongation factor like GTPase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1430.10:FF:000002">
        <property type="entry name" value="Elongation factor like GTPase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000746">
        <property type="entry name" value="Ribosome assembly protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.870:FF:000002">
        <property type="entry name" value="Translation elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.870">
        <property type="entry name" value="Elongation Factor G (Translational Gtpase), domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041095">
        <property type="entry name" value="EFG_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056752">
        <property type="entry name" value="EFL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908:SF3">
        <property type="entry name" value="ELONGATION FACTOR-LIKE GTPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908">
        <property type="entry name" value="TRANSLATION ELONGATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14492">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25118">
        <property type="entry name" value="EFL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Tr-type G" evidence="11">
        <location>
            <begin position="17"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="478"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="478"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00068031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00081809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR21634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1100" mass="122735" checksum="F8E7228696CE3FD4" modified="2009-07-28" version="1">MSRVGPETSKRLQKDPSCIRNICILAHVDHGKTSLSDSLLASNGIISQKLAGKVRFLDSRPDEQLRGITMESSAISLYFRVLRKQEGKDEPLVNEHIINLIDSPGHIDFSSEVSAASRLCDGAVVLVDVVEGVCSQTITVLRQCWIEKLKPVLVLNKIDRLVTELQLTPQEAYVHLNKIIEQVNSIIGSFFNGQRLLDDLSWREQLENNTNAEFVEKDDTDIYFKPENNNVVFTSAVDGWGFNIGQIAKFYESKLGAKRENLQKVLWGDFYIDPKTKKFINSKGLKGRTLKPLFVSLILDNIWKIYNNVLVERNADVLEKITQTLNIKVSQRDLRSKDLKNLLRTIMGQWLPVSTSILLTVIEKVPSPLESQSEKMEIIIGNAPGSDLIDKGLYKSMESCDKDGPVCAYVSKMLSIPKEELPINNGKPLSQGELMERSRAAREQALKAAKAAELAESLSRTNASKDEGRGFYERAQETVATPQLGTANHSADNTTSKPSETDTFKIVNEPAQEFDLGLDLGDEDYEDEDDINLEPEFSPAVIDPNDPLSSMFEYEEEDPFETNNEAELEEDDAGDFEESDEVMIGFARLYSGTLKVGQKVSVLGPKYDPRFPADHIEQVEITDLFIFMGKELVPLTECPPGNIVGIGGLAGKILKNGTIVDPQVQGANLAGVNLHVTPIVRVALEPTNPTQMGRLARGLKLLDQADPCVQTYVEDTGEHILCTAGELHLERCLKDLRERFAGIGITSSKPAIPYKETFLSTSDMNPPKNAELGRGVCQRYLGEYAFKFRSAPLPENITQYLTEQEANIKSLVEEKQQSNESSEFSKNLKNLFTEAAAKNKIWDQCNSCISAFGPKRAGPNILIAENVLSGQKDGEETSDTFEYKSSIINGFELAVSGGPLAKEPVSGMCVIVEDIHKMSQEEIDQLEVQNYQAEVPNFSGRLITTARDIIHSTFLDWSPRIMWAMYSCEIQASVEVLGKVYAVVQQRRGKIVSEEMKEGIPLFQIEAQIPVVEAFGFSEDIRKKTSGAAQPQLVFAGFECIDLDPFWVPTTEEELEELGDTADKENIARKHMNMIRRRKGLFVDEKIVQNAEKQRTLKRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCX4</accession>
    <name>C5DCX4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein IBD2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0B06534g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle pole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IBD2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552073.1">
        <property type="nucleotide sequence ID" value="XM_002552027.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCX4"/>
    <dbReference type="FunCoup" id="C5DCX4">
        <property type="interactions" value="34"/>
    </dbReference>
    <dbReference type="GeneID" id="8290912"/>
    <dbReference type="KEGG" id="lth:KLTH0B06534g"/>
    <dbReference type="eggNOG" id="ENOG502RXXW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067888_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCX4"/>
    <dbReference type="OMA" id="PKNMIKW"/>
    <dbReference type="OrthoDB" id="4057723at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026231">
        <property type="entry name" value="IBD2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02099">
        <property type="entry name" value="PROTEINIBD2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="58"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="167"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="108"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="169"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="192"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="211"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="222"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR21635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="299" mass="33742" checksum="827F9CD1EEAB1CAB" modified="2009-07-28" version="1">MCPSNNTSIEVVSKDGPVDFNVMMQEGVKALTKVLADHLQDHPDYLKQQSMKLVFNNDIDKSGENGEAAGTSEEDAKQITATKSAGDEEIEEADLRNKSNYHMCKEMSPQTHKLSSLYSPSKNKQSPSIAQPSEFDCEEGELVFDYGEQDLMSLPGEFGDSIKKMVASSMAQDPSGRHSNINIDLDVDDTLSDNNDSPEPNSFSDQPSQKQHGHQKHRRCRHGGDSNREHEFEYPHHPKVTPDFASLITYDQPLCMFCEYYFVFGEPPKNMIKWFQRTGGRDTSAHCRSYQRHSNSTQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DD44</accession>
    <name>C5DD44_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0B08162p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0B08162g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552143.1">
        <property type="nucleotide sequence ID" value="XM_002552097.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DD44"/>
    <dbReference type="FunCoup" id="C5DD44">
        <property type="interactions" value="149"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DD44"/>
    <dbReference type="GeneID" id="8290983"/>
    <dbReference type="KEGG" id="lth:KLTH0B08162g"/>
    <dbReference type="eggNOG" id="KOG3322">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007205_0_1_1"/>
    <dbReference type="InParanoid" id="C5DD44"/>
    <dbReference type="OMA" id="WNAKRSH"/>
    <dbReference type="OrthoDB" id="442863at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005655">
        <property type="term" value="C:nucleolar ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.120">
        <property type="entry name" value="Probable tRNA modification gtpase trme, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039182">
        <property type="entry name" value="Pop1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055079">
        <property type="entry name" value="POP1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009723">
        <property type="entry name" value="Pop1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012590">
        <property type="entry name" value="POPLD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027266">
        <property type="entry name" value="TrmE/GcvT-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22731">
        <property type="entry name" value="RIBONUCLEASES P/MRP PROTEIN SUBUNIT POP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22731:SF3">
        <property type="entry name" value="RIBONUCLEASES P_MRP PROTEIN SUBUNIT POP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22770">
        <property type="entry name" value="POP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06978">
        <property type="entry name" value="POP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08170">
        <property type="entry name" value="POPLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103025">
        <property type="entry name" value="Folate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Pop1 N-terminal" evidence="5">
        <location>
            <begin position="51"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="POPLD" evidence="6">
        <location>
            <begin position="528"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="domain" description="POP1 C-terminal" evidence="7">
        <location>
            <begin position="787"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="108"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF06978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF22770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR21705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="844" mass="96361" checksum="A901A39A43EAA26D" modified="2009-07-28" version="1">MSGKKPRSQNQLYRRRKVQDARNIRTEALKSGAQASQELSESGGMMRVPEFMASREFEVKQLQVAMHKSKSASSTRVFQSLPRKLRRRTASHNVKRIPKRLRNRALREMQKSDQAVTRGTEKPSRQRRSGLTAKQLYKARMTVKLLRLAARSKSMKLALPAQVTGSQTKLRQRIRALQKTLRAEAKPGTAQLRNNQMGSYDCTGIGKLAPRPVGRIKYMKRQRVFTWLPTHVWNAKRSHMLKRWGYQMPWSPTQKCFKLTHRIGGNTAASDGALCCDSSYMGTMIVASADTEALRLLAHQLTNKRATLPKYRVSGHWFEGLIYDENAEAVGPGEILWISSNKLLLRLHPAIYSRVFEQISLANSEKKFVIQDCRFSIGSITLSGGKSLNALSQVLRSTTASKSYNQFKTVAYVTDTNVLPQRTVFAFEAIDPRHLSNPRALPKANINANDVLKLQAEYPREEIAEVLEKLCNPDKREMSYKNQQTLKQLAYRRRMLLESTARTNAIPHDSKEDPFIPIAIFKRPKQESWVVLLPWFWLLPLWYQLNRVSRVYHMGLRQLQQLSFEQRRLYFPDDYPFTQAGYDENSTFKRLSQEALWKRKPAGKRVNFDKISGIHTENPVRFPGEIGDPFSCDWRLLQVLRNGIDFLRTRDQGPIRMYDPKRTSQFDELNMRKLMYVNDIIDLYSDVQDKKLTPSALPISIFTKSVKGANSGCPQYGNSPRMQIVTTPLQVAAVSCVLQEQGHPKDFARVYAIPAQDMDYWLSIVNIKARSNGKRDHDTAQPTPGVQDLIGFVTSGTFHLGDGRGACTAFIAAQEACKSPNEYVLIRNVGTNVYRVAKWEQIAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>C5DD99</accession>
    <name>C5DD99_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Amidophosphoribosyltransferase</fullName>
            <shortName evidence="8">ATase</shortName>
            <ecNumber evidence="3 8">2.4.2.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Glutamine phosphoribosylpyrophosphate amidotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0B09460g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>5-phospho-beta-D-ribosylamine + L-glutamate + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + L-glutamine + H2O</text>
            <dbReference type="Rhea" id="RHEA:14905"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58681"/>
            <dbReference type="EC" id="2.4.2.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="10">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.14" evidence="3 8"/>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552198.1">
        <property type="nucleotide sequence ID" value="XM_002552152.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DD99"/>
    <dbReference type="FunCoup" id="C5DD99">
        <property type="interactions" value="773"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DD99"/>
    <dbReference type="MEROPS" id="C44.001"/>
    <dbReference type="GeneID" id="8291041"/>
    <dbReference type="KEGG" id="lth:KLTH0B09460g"/>
    <dbReference type="eggNOG" id="KOG0572">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022389_2_1_1"/>
    <dbReference type="InParanoid" id="C5DD99"/>
    <dbReference type="OMA" id="IRHFGVK"/>
    <dbReference type="OrthoDB" id="191723at2759"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00124"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004044">
        <property type="term" value="F:amidophosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00715">
        <property type="entry name" value="GPATase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01931">
        <property type="entry name" value="PurF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005854">
        <property type="entry name" value="PurF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035584">
        <property type="entry name" value="PurF_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01134">
        <property type="entry name" value="purF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11907">
        <property type="entry name" value="AMIDOPHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13522">
        <property type="entry name" value="GATase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000485">
        <property type="entry name" value="Amd_phspho_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="4 8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 8" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="11">
        <location>
            <begin position="2"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="9">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="311"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="373"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="374"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR21760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="510" mass="56433" checksum="FBF27EACF695DC20" modified="2009-07-28" version="1">MCGVLGIALGDQTSAVAPELFDGCLFLQHRGQDAAGIATCGQRGRLYQCKGNGMARDVFTQQRMANLVGSMGIAHLRYPTAGSSANSEAQPFYVNSPYGILLGHNGNLVNTLSLRRYMDEDVHRHINTDSDSELLLNILAAELEKHNKYRVNNDDIFHALEGVYRLCRGGYACVGMLAGYAMIGFRDPNGIRPLLFGERTRPDGTKDYMLASESVVLKAHNFNNFRDLAPGEAVIVPKDCGNTAPEFKQVVPINSYRPDLFEYVYFARPDSVLDGISVYHTRLAMGVKLAESIRKEIDPNDIDVVVSVPDTARTCALQCANTLQIPYREGFVKNRYVGRTFIMPNQKERVSSVRRKLNAMDSEFKGKRVLVVDDSIVRGTTSKEIISMAKEAGASKVYFASAAPAIRYNHIYGIDLADTKQLVAFDKSTEEVSQELGCDRVFYQSLEDLVDCCKTDKISKFEVGVFTGNYVTGVEDGYLQELEKVRALNAAKQNFAKADSDIGLYNSADF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDH9</accession>
    <name>C5DDH9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0C01122p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0C01122g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_002552232.1"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="InParanoid" id="C5DDH9"/>
    <dbReference type="OMA" id="QVIWADA"/>
    <dbReference type="OrthoDB" id="5817230at2759"/>
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        <property type="component" value="Chromosome C"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:G protein-coupled receptor binding"/>
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        <property type="term" value="F:G-protein beta/gamma-subunit complex binding"/>
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    </dbReference>
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        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:pheromone-dependent signal transduction involved in conjugation with cellular fusion"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Guanine nucleotide-binding protein alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002975">
        <property type="entry name" value="Fungi_Gprotein_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001019">
        <property type="entry name" value="Gprotein_alpha_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011025">
        <property type="entry name" value="GproteinA_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218">
        <property type="entry name" value="GTP-BINDING PROTEIN ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218:SF302">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN ALPHA-5 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00503">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00318">
        <property type="entry name" value="GPROTEINA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01241">
        <property type="entry name" value="GPROTEINAFNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00275">
        <property type="entry name" value="G_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47895">
        <property type="entry name" value="Transducin (alpha subunit), insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51882">
        <property type="entry name" value="G_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0807">Transducer</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="51"/>
            <end position="56"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="263"/>
            <end position="269"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="288"/>
            <end position="292"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="357"/>
            <end position="360"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="413"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR21840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="441" mass="50051" checksum="50C371D081BA64BE" modified="2009-07-28" version="1">MGCAASTALSADEQDPFLQSKRANDMVERSLQMQKHKEKKEIKLLLLGAGESGKSTVLKQLRLLHQGGFGNQERVQYTQVIWADAVQSMKILIIQARKLGIPLDCDNPETNRHLFEQKRLLLRARPLEHIDASLAGGSGFLNDYVLKYSETSENKRRTKSTGRAQAFQGLEDGDTFNLQELSEGLSEDGTNSALAQSQNVATRSVSSQQIADAISELWKHDRGVRQCFARSNEFQLESSAEYYFEHIHNFANPTYVCSDEDILKGRIKTTGITETAFNIGSTNFKVLDAGGQRSERRKWIHCFQDITAVLFVLAVSEYDQMLFEDERVNRMHESIMLFNTLLNSKWFSNTPFILFLNKTDIFKEKLTRSPIRQYFPEYQGRVGDVDAGLKYFESIFLSLNKTGRPVYVHRTCATDTQSMRFVLTAVADLIIQQNLKKSGIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDU5</accession>
    <name>C5DDU5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">phosphopentomutase</fullName>
            <ecNumber evidence="14">5.4.2.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ordered locus">KLTH0C03872g</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>alpha-D-ribose 1-phosphate = D-ribose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:18793"/>
            <dbReference type="ChEBI" id="CHEBI:57720"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="EC" id="5.4.2.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.7" evidence="14"/>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552394.1">
        <property type="nucleotide sequence ID" value="XM_002552348.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDU5"/>
    <dbReference type="FunCoup" id="C5DDU5">
        <property type="interactions" value="345"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDU5"/>
    <dbReference type="GeneID" id="8291258"/>
    <dbReference type="KEGG" id="lth:KLTH0C03872g"/>
    <dbReference type="eggNOG" id="KOG1220">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016950_0_1_1"/>
    <dbReference type="InParanoid" id="C5DDU5"/>
    <dbReference type="OMA" id="GYCVDPE"/>
    <dbReference type="OrthoDB" id="8300170at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008973">
        <property type="term" value="F:phosphopentomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05799">
        <property type="entry name" value="PGM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000035">
        <property type="entry name" value="Pgm3p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000037">
        <property type="entry name" value="Pgm3p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.120.10">
        <property type="entry name" value="Alpha-D-Glucose-1,6-Bisphosphate, subunit A, domain 3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.50">
        <property type="entry name" value="Alpha-D-phosphohexomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005845">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005846">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005843">
        <property type="entry name" value="A-D-PHexomutase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016066">
        <property type="entry name" value="A-D-PHexomutase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45745:SF1">
        <property type="entry name" value="PHOSPHOGLUCOMUTASE 2B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45745">
        <property type="entry name" value="PHOSPHOMANNOMUTASE 45A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02879">
        <property type="entry name" value="PGM_PMM_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02880">
        <property type="entry name" value="PGM_PMM_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00408">
        <property type="entry name" value="PGM_PMM_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="Phosphoglucomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="Phosphoglucomutase, first 3 domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00710">
        <property type="entry name" value="PGM_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0313">Glucose metabolism</keyword>
    <keyword evidence="10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="16">
        <location>
            <begin position="53"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="17">
        <location>
            <begin position="229"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="18">
        <location>
            <begin position="348"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase C-terminal" evidence="15">
        <location>
            <begin position="566"/>
            <end position="594"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00051394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00066543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAR21956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="621" mass="70174" checksum="621796B055426A63" modified="2009-07-28" version="1">MMSLTNAEVPEDLKLGVEAWLKYDKNEQTRDQIIKLVEDKSWTELRNRLNFRITFGTAGLRSRMEAGFNRMNTLTVLQASQGLAKYIASNFPDNLSVVVGHDHRFNSKDFAEVTIVAFLQLGFKVYDLGFTEDASQDVVVHTPMVPFGIDHLRASGGIMITASHNPKMDNGYKVYYSNGCQIIPPHDQRISQSILANLEPWDRAWDCAAVKAEAVQSEMLIDAKKVLVDQYVDTVRRSLVITNVNHTKAPWFVYTPLHGVGFEIFEKILSSAWRLGQGRDYLCVEEQKYPDPNFPTVSFPNPEEKGALDKAIELAEKHDITFVLGNDPDADRFSVAAKHNGQWRQLTGNEIGFLFAQYKWETYRLESDKFKGTNPLAMINSTVSSQMIKKMAEVEGFHYEDTLTGFKWLGNRARELENAGYFVPFAYEEAIGYMFSDVVHDKDGISAAVVFLQMLWSWKAQALTPFDVLQRAYQKYGVFKEYNGYYIVPDLSLTDKVFSGIRSGFSLQPTHIGQEFRVESYTDLTTGYQSNTPDSEPRLPVDPSSQMITATLTPTSSTGRQGFSESVRFTARGSGTEPKLKVYIEAVSSSEELASALAKKAWDVLRREWFKPEQTGLATNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DE88</accession>
    <name>C5DE88_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">KLTH0C07172p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0C07172g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552537.1">
        <property type="nucleotide sequence ID" value="XM_002552491.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DE88"/>
    <dbReference type="STRING" id="559295.C5DE88"/>
    <dbReference type="GeneID" id="8291406"/>
    <dbReference type="KEGG" id="lth:KLTH0C07172g"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1049979_0_0_1"/>
    <dbReference type="InParanoid" id="C5DE88"/>
    <dbReference type="OMA" id="HERKHYE"/>
    <dbReference type="OrthoDB" id="6365676at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043709">
        <property type="term" value="P:cell adhesion involved in single-species biofilm formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000218">
        <property type="term" value="P:negative regulation of invasive growth in response to glucose limitation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000221">
        <property type="term" value="P:negative regulation of pseudohyphal growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001382">
        <property type="entry name" value="Transcriptional repressor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR PROTEIN YY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003:SF19">
        <property type="entry name" value="YY2 TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="202"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="230"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="96"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="160"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="96"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="107"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="160"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR22099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="265" mass="27392" checksum="93378C7226F82C09" modified="2009-07-28" version="1">MTTLASMHSPALAGAVRSSVEEFYYRGSPSTCEQRCIALDGARGNQVLLPGPGSGPVTAAQRHVESDLKRALDRCAFGASTALAATTTLPPLRLARTGSVTPHGSVSGPGVGSGSGSGTNLALVPGLRARAESASGSVSASGSDYDSAAGPVSPVTAALSPATAPAAGETSAPRPASAMSSATTSRPTGKRARSPIEQRRRYVCKTCVKGFTTSGHLARHNRIHTGEKNHVCPHPGCGQRFSRHDNCVQHYKTHLRRNTWQDDAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEA5</accession>
    <name>C5DEA5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0C07612p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0C07612g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552554.1">
        <property type="nucleotide sequence ID" value="XM_002552508.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEA5"/>
    <dbReference type="FunCoup" id="C5DEA5">
        <property type="interactions" value="26"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEA5"/>
    <dbReference type="GeneID" id="8291426"/>
    <dbReference type="KEGG" id="lth:KLTH0C07612g"/>
    <dbReference type="eggNOG" id="ENOG502S4BW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_160816_0_0_1"/>
    <dbReference type="InParanoid" id="C5DEA5"/>
    <dbReference type="OMA" id="LAFHQCM"/>
    <dbReference type="OrthoDB" id="276296at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045333">
        <property type="term" value="P:cellular respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2900">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052848">
        <property type="entry name" value="CHCH_domain-containing_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036549">
        <property type="entry name" value="CX6/COA6-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031731">
        <property type="entry name" value="CX9C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016611">
        <property type="entry name" value="Mix14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47106">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47106:SF1">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16860">
        <property type="entry name" value="CX9C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF013232">
        <property type="entry name" value="UCP013232"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47694">
        <property type="entry name" value="Cytochrome c oxidase subunit h"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IMS import disulfide relay-system CHCH-CHCH-like Cx9C" evidence="3">
        <location>
            <begin position="10"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="IMS import disulfide relay-system CHCH-CHCH-like Cx9C" evidence="3">
        <location>
            <begin position="57"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR22116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="111" mass="12717" checksum="390CFC838625856E" modified="2009-07-28" version="1">MSNALDQFIMEDVAKNCPQQFVEYHKCISKNKDDPGQCAFRQRDLSVCIKQKVPSVQKVMAKCGQLMQKYETCVRDNMETRTINENCLGLLQEMRTCAEKQVQGPSPINQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>C5DED3</accession>
    <name>C5DED3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">pyridoxal 5'-phosphate synthase</fullName>
            <ecNumber evidence="7">1.4.3.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">PNP/PMP oxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">KLTH0C08272g</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the oxidation of either pyridoxine 5'-phosphate (PNP) or pyridoxamine 5'-phosphate (PMP) into pyridoxal 5'-phosphate (PLP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>pyridoxamine 5'-phosphate + O2 + H2O = pyridoxal 5'-phosphate + H2O2 + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15817"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:58451"/>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
            <dbReference type="EC" id="1.4.3.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>pyridoxine 5'-phosphate + O2 = pyridoxal 5'-phosphate + H2O2</text>
            <dbReference type="Rhea" id="RHEA:15149"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:58589"/>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
            <dbReference type="EC" id="1.4.3.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor metabolism; pyridoxal 5'-phosphate salvage; pyridoxal 5'-phosphate from pyridoxamine 5'-phosphate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor metabolism; pyridoxal 5'-phosphate salvage; pyridoxal 5'-phosphate from pyridoxine 5'-phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the pyridoxamine 5'-phosphate oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.5" evidence="7"/>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552582.1">
        <property type="nucleotide sequence ID" value="XM_002552536.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DED3"/>
    <dbReference type="FunCoup" id="C5DED3">
        <property type="interactions" value="453"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DED3"/>
    <dbReference type="GeneID" id="8291456"/>
    <dbReference type="KEGG" id="lth:KLTH0C08272g"/>
    <dbReference type="eggNOG" id="KOG2586">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032263_2_0_1"/>
    <dbReference type="InParanoid" id="C5DED3"/>
    <dbReference type="OMA" id="AYFRTRP"/>
    <dbReference type="OrthoDB" id="303614at2759"/>
    <dbReference type="UniPathway" id="UPA01068">
        <property type="reaction ID" value="UER00304"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004733">
        <property type="term" value="F:pyridoxamine phosphate oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008615">
        <property type="term" value="P:pyridoxine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.110.10:FF:000010">
        <property type="entry name" value="Pyridoxine phosphate oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01629">
        <property type="entry name" value="PdxH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000659">
        <property type="entry name" value="Pyridox_Oxase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019740">
        <property type="entry name" value="Pyridox_Oxase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011576">
        <property type="entry name" value="Pyridox_Oxase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019576">
        <property type="entry name" value="Pyridoxamine_oxidase_dimer_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00558">
        <property type="entry name" value="pdxH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004231">
        <property type="entry name" value="PRK05679.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10851:SF0">
        <property type="entry name" value="PYRIDOXINE-5'-PHOSPHATE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10851">
        <property type="entry name" value="PYRIDOXINE-5-PHOSPHATE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10590">
        <property type="entry name" value="PNP_phzG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01243">
        <property type="entry name" value="PNPOx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000190">
        <property type="entry name" value="Pyd_amn-ph_oxd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01064">
        <property type="entry name" value="PYRIDOX_OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9" id="KW-0288">FMN</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-0664">Pyridoxine biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyridoxamine 5'-phosphate oxidase N-terminal" evidence="15">
        <location>
            <begin position="56"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="Pyridoxine 5'-phosphate oxidase dimerisation C-terminal" evidence="16">
        <location>
            <begin position="184"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00050700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00052480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00083138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF10590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAR22144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="226" mass="26230" checksum="49C4174A792A3C38" modified="2009-07-28" version="1">MASDETKNPIIFAPETYQYNKDSLNESELAQDPIVEFTKWFADAKNDPTEMIPEAVNFSSAELPSGRVSSRILLFKELDERGFTIYSNWETSRKAQDIRSNPQAALTFFWRNLQRQVRVEGIVEKVSRETSERYFNTRPRGSKVGAWSSPQSAPLKDRAELQKLVDDNEARFEGVEEVPCPDFWGGVRIVPLMIEFWQGRPSRLHDRIVFKRESEKDPWQMLRIAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFD7</accession>
    <name>C5DFD7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">KLTH0D14278p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0D14278g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peptidase M16 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002553284.1"/>
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        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
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        <property type="entry name" value="Putative insulin-degrading enzyme"/>
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        <property type="entry name" value="INSULIN-DEGRADING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NARDILYSIN"/>
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        <property type="entry name" value="Peptidase_M16"/>
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    </dbReference>
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        <property type="entry name" value="Peptidase_M16_M"/>
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        <property type="entry name" value="PqqF-like_C_4"/>
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        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00143">
        <property type="entry name" value="INSULINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="8">
        <location>
            <begin position="69"/>
            <end position="205"/>
        </location>
    </feature>
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        <location>
            <begin position="232"/>
            <end position="409"/>
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    </feature>
    <feature type="domain" description="Peptidase M16 middle/third" evidence="10">
        <location>
            <begin position="416"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="domain" description="Coenzyme PQQ synthesis protein F-like C-terminal lobe" evidence="11">
        <location>
            <begin position="803"/>
            <end position="900"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00007261"/>
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    <evidence type="ECO:0000256" key="2">
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            <dbReference type="ARBA" id="ARBA00022670"/>
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            <dbReference type="ARBA" id="ARBA00022723"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU004447"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF05193"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF16187"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF22456"/>
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    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR22892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1001" mass="114339" checksum="169D3CD5363B4477" modified="2009-07-28" version="1">MNTVLPGCSLVYKSKHLFSNSANSLCSHVKGTLIHRKMSSYIDHGVKFTKPLLDDRNYRFIELPNKLKALLIQDPTTDKAAAALDVNVGSFEDPEDLPGLAHFCEHLLFMGSSKFPNENEYSSYLSKHGGGSNAYTSARNTNYFFQVNQESLHGALLRFSGFFSCPLFNKESTDKEINAVDSENKKNLQSDLWRLYQLDKSQSNPEHPFHKFSTGNLKTLGDIPKSKDVDIRDELLKFYDSSYSANLMKLCVLGREDLDTMSQWVYDLFKDVPNSDRPVPTYEAKMLPPQYLTQIINAKPVKDLKKVEITFVAPDVDEQWDSKPGHYLSHLIGHEGSGSLLAYLKLKGWANELSAGSHTVSEDNAFFSVDIDLTDEGVKNYESVIQSVFQYIELLKKELPQEWIYEELRDTAEASFKFKQKGNPASTVSSLSKALEKDYIPVGDVLSTSLLRKYEPELVVKYIAHLIPENSRVTFIHKNASTDSTEKWYGTEYGVVKYSEGLMDKLKSPGTNSLLHLPRRNEFISSNFQVDKLEDVEPLQEPLLLKQDRQSKVWYKKDDRFWVPKGHIYVSMKLAHTYSSVVNSMLTTLYVELINDYLKDLEYDAQVASLHISFRKTNQGLDLSLSGYNEKMAILLKRYLEGIANFQPAEDRFKIYQDKLLQKLNNHLYEVPYSQVSDVFNSVINERAWTIANKLEVVKQLKFEHLKLFIPAIFEQFSFEILVHGNFSCEAALEADNLVRALAPRDVQNFQLKSSKPRSVLLPQGKTFCYQQMLADDKNINSCIQHVTQFGSYSEELSAKASLFAQLIDEPAFDTLRTKEQLGYVVFSSALNTHGTVNLRLLIQSERDTAYLESRIDAFLVKMGQVLQEMSDEEFERHKVALCKTLLQRYKNLSEENTRFTTAIYIGDYNFINKERKASLVEKLSKQDMLEFYSQYVVTEEASKLVIHLKSQAISDEQKSNDRVEGYPTGELITDIGSFQSKLCLAPVRQPIKKLEVTPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFJ8</accession>
    <name>C5DFJ8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitochondrial intermembrane space import and assembly protein 40</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Mitochondrial import inner membrane translocase TIM40</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="ordered locus">KLTH0D15686g</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Cu(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29036"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion inner membrane</location>
            <topology evidence="3">Single-pass type II membrane protein</topology>
            <orientation evidence="3">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553391.1">
        <property type="nucleotide sequence ID" value="XM_002553345.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFJ8"/>
    <dbReference type="STRING" id="559295.C5DFJ8"/>
    <dbReference type="GeneID" id="8295637"/>
    <dbReference type="KEGG" id="lth:KLTH0D15686g"/>
    <dbReference type="eggNOG" id="KOG4149">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054990_3_1_1"/>
    <dbReference type="InParanoid" id="C5DFJ8"/>
    <dbReference type="OrthoDB" id="7481291at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045041">
        <property type="term" value="P:protein import into mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.2900:FF:000002">
        <property type="entry name" value="Mitochondrial intermembrane space import and assembly protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010625">
        <property type="entry name" value="CHCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039289">
        <property type="entry name" value="CHCHD4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21622">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN CONTAINING 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21622:SF0">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN CONTAINING 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06747">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="13" id="KW-0811">Translocation</keyword>
    <keyword evidence="6 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="CHCH" evidence="21">
        <location>
            <begin position="125"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="79"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="171"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="178"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="201"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="224"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00033150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF06747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="CAR22953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="236" mass="26141" checksum="8136A4FC04A2A4CD" modified="2009-07-28" version="1">MYRTGLLGVRRLNLFRQSFAQRRLLSYGNVNRQFATSRFRLASASVAAGSMLGMLAAGSYLALEGSEKIEAEKEALAVVSEKGETEKKGQNGDDSEKKPQSAYDPDTGEINWDCPCLGGMAQGPCGEEFKAAFSCFVYSEEDPKGIDCIEKFKGMQDCFRKYPEHYAEQIKDEEDAAEAAAQLESQSSDQSQPPSEAKMTSPEETKPMKQQEADFEHLEEELKEDTKSSDKGVKPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFN7</accession>
    <name>C5DFN7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">V-type proton ATPase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0D16610g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Subunit of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments. This subunit is a non-integral membrane component of the membrane pore domain and is required for proper assembly of the V0 sector. Might be involved in the regulated assembly of V1 subunits onto the membrane sector or alternatively may prevent the passage of protons through V0 pores.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'', d, e, f and VOA1).</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">V-ATPase is a heteromultimeric enzyme made up of two complexes: the ATP-hydrolytic V1 complex and the proton translocation V0 complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the V-ATPase V0D/AC39 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553430.1">
        <property type="nucleotide sequence ID" value="XM_002553384.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFN7"/>
    <dbReference type="FunCoup" id="C5DFN7">
        <property type="interactions" value="900"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFN7"/>
    <dbReference type="GeneID" id="8295678"/>
    <dbReference type="KEGG" id="lth:KLTH0D16610g"/>
    <dbReference type="eggNOG" id="KOG2957">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051277_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFN7"/>
    <dbReference type="OMA" id="MTYGYMI"/>
    <dbReference type="OrthoDB" id="10250083at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1690.10:FF:000003">
        <property type="entry name" value="V-type proton ATPase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.50">
        <property type="entry name" value="ATP synthase (C/AC39) subunit, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1690.10">
        <property type="entry name" value="V-type ATP synthase subunit C domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036079">
        <property type="entry name" value="ATPase_csu/dsu_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002843">
        <property type="entry name" value="ATPase_V0-cplx_csu/dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016727">
        <property type="entry name" value="ATPase_V0-cplx_dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035067">
        <property type="entry name" value="V-type_ATPase_csu/dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044911">
        <property type="entry name" value="V-type_ATPase_csu/dsu_dom_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11028">
        <property type="entry name" value="VACUOLAR ATP SYNTHASE SUBUNIT AC39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01992">
        <property type="entry name" value="vATP-synt_AC39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018497">
        <property type="entry name" value="V-ATP_synth_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103486">
        <property type="entry name" value="V-type ATP synthase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="4 7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00059115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR018497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR22992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="347" mass="39909" checksum="7FC172581D9B7A82" modified="2009-07-28" version="1">MEGVFFNVDNGFIEGVVRGYRNGLLTGNQYINLTQCDTLEDLKLQLSSTDYGNFLSSVASDALTTSIIQDKASAKLYEEFQYIRDQSSSVTKKFLDYITYGYMIDNVALMITGTIHDRDKAEILPRCHPLGWFDTLPTLTVATDLESLYETVLVDTPLAPYFRDCFDAADELDDVNIEIIRNKLYKAYLSDFYEFVSTEIEEPAREVLQTFLSFEADRRAINISLNSLQSGDLINPDMKRDLLPTFGKLYPVGTEQLASNGSDFEAVRSIVENVYEYRGIFETGNLEDHFYKMEMELCRDAFTQQFTLSTIWAWMKSKEQEVRNITWIAECIAQNQRERVNNYISVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFX2</accession>
    <name>C5DFX2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0D00660p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0D00660g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552752.1">
        <property type="nucleotide sequence ID" value="XM_002552706.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFX2"/>
    <dbReference type="STRING" id="559295.C5DFX2"/>
    <dbReference type="GeneID" id="8294972"/>
    <dbReference type="KEGG" id="lth:KLTH0D00660g"/>
    <dbReference type="HOGENOM" id="CLU_1970938_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFX2"/>
    <dbReference type="OrthoDB" id="4036424at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="49"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR22314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="146" mass="15924" checksum="2FF9A1A3DEC03F88" modified="2009-07-28" version="1">MRTLEDITIGGSTYTMADKDALRLGETAQSIVNKIFSRFMLLDALFRGMVFGVGVLVVVFLTAVLLIKIMLASQENGKAKAKSGNRNTSPSSVDNAKSGDQKSRNAKSSALNDEDHAHLLSRKVIPINSTDDFTEELEISVMEEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>C5DG09</accession>
    <name>C5DG09_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 17">Pyruvate kinase</fullName>
            <ecNumber evidence="6 17">2.7.1.40</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ordered locus">KLTH0D01496g</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16 17">
            <text>pyruvate + ATP = phosphoenolpyruvate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18157"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.40"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 17">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">Belongs to the pyruvate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.40" evidence="6 17"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552789.1">
        <property type="nucleotide sequence ID" value="XM_002552743.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DG09"/>
    <dbReference type="FunCoup" id="C5DG09">
        <property type="interactions" value="1034"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DG09"/>
    <dbReference type="GeneID" id="8295009"/>
    <dbReference type="KEGG" id="lth:KLTH0D01496g"/>
    <dbReference type="eggNOG" id="KOG2323">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015439_0_1_1"/>
    <dbReference type="InParanoid" id="C5DG09"/>
    <dbReference type="OMA" id="RVHHIGE"/>
    <dbReference type="OrthoDB" id="108365at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00188"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030955">
        <property type="term" value="F:potassium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004743">
        <property type="term" value="F:pyruvate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00288">
        <property type="entry name" value="Pyruvate_Kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.33.10:FF:000001">
        <property type="entry name" value="Pyruvate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.60:FF:000001">
        <property type="entry name" value="Pyruvate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1380.20:FF:000001">
        <property type="entry name" value="Pyruvate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.33.10">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1380.20">
        <property type="entry name" value="Pyruvate kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001697">
        <property type="entry name" value="Pyr_Knase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011037">
        <property type="entry name" value="Pyrv_Knase-like_insert_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018209">
        <property type="entry name" value="Pyrv_Knase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015793">
        <property type="entry name" value="Pyrv_Knase_brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015795">
        <property type="entry name" value="Pyrv_Knase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036918">
        <property type="entry name" value="Pyrv_Knase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015806">
        <property type="entry name" value="Pyrv_Knase_insert_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004491">
        <property type="entry name" value="PRK05826.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004978">
        <property type="entry name" value="PRK06354.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01064">
        <property type="entry name" value="pyruv_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11817">
        <property type="entry name" value="PYRUVATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00224">
        <property type="entry name" value="PK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02887">
        <property type="entry name" value="PK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01050">
        <property type="entry name" value="PYRUVTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50800">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52935">
        <property type="entry name" value="PK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00110">
        <property type="entry name" value="PYRUVATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14 17" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="10 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="12 17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0630">Potassium</keyword>
    <keyword evidence="15" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 17" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate kinase barrel" evidence="18">
        <location>
            <begin position="20"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate kinase C-terminal" evidence="19">
        <location>
            <begin position="381"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CAR22351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="501" mass="54794" checksum="39F016EE133D058B" modified="2009-07-28" version="1">MESRLGWLSNLQTDTGSNLRRTSIIGTIGPKTNSPETLVALRKAGLNIVRMNFSHGSYEYHQSVIDNARKSEELYPGRPLAIALDTKGPEIRTGTTTNEVDYPIPPNHEMIFTIDDKYAKSSDDKIMYIDYKNITKVIEKGRVIYVDDGVLSFEVLEVVDDKTLKVKSLNAGKICSHKGVNLPGTDVDLPALSEKDKADLRFGVKNGVHMVFASFIRTANDVKTIREVLGEDGKDIKIIVKIENQQGVNNFDEILEVTDGVMVARGDLGIEIPAPQVLAVQKKLIAKCNLAGKPVVCATQMLESMTYNPRPTRAEVSDVGNAVLDGADCVMLSGETAKGNYPINAVTMMAETALMAEQAIPYIPTYDDLRNCTPKPTSTTETIAAAAVASVFEQRAKAIIVLSTTGSTPRLVSKYKPNVPIILVTRNPRTARISHLSRGVFPFVYEKGTESDWTKDVESRLQFGIEQAKEFGMLKEGDTIVTIQGFAAGVGHSNTLRVLTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGC0</accession>
    <name>C5DGC0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0D04070p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0D04070g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552900.1">
        <property type="nucleotide sequence ID" value="XM_002552854.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGC0"/>
    <dbReference type="STRING" id="559295.C5DGC0"/>
    <dbReference type="GeneID" id="8295125"/>
    <dbReference type="KEGG" id="lth:KLTH0D04070g"/>
    <dbReference type="eggNOG" id="ENOG502QSJ0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054069_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGC0"/>
    <dbReference type="OMA" id="FMKASPV"/>
    <dbReference type="OrthoDB" id="504708at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018959">
        <property type="entry name" value="DUF1989"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31527">
        <property type="entry name" value="RE64534P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31527:SF0">
        <property type="entry name" value="RE64534P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09347">
        <property type="entry name" value="DUF1989"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1989" evidence="2">
        <location>
            <begin position="57"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="281"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR22462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="300" mass="33643" checksum="97667A95291A905D" modified="2009-07-28" version="1">MSSNLTFVSQRSEKKNYGKPKPIPAYFPTSKKSILIPNFEFYDRVKAAPRKLAATHAVPVRSGKAWKASKGSIVRISTPEGPQVCDFNCWEANSGFKEHMWASRTRQLHSAHVSVYDKLWSNLPYLRPLLTIIDDSLAGYGPDEHGGRVHDLLGTRCDPYVDKLLSGEDNDFHCHSNLYRAIREFGGEEEHVHDVLNIFQLTGLNENDQYFMETCPAKPGDYFEFFCEVDVVCAVSSCPGGDLSDWGWGEGGTDSEANVGDMSAKCRPLKVEVFELENPEEALEGWESPQPVKYQGNHGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGI9</accession>
    <name>C5DGI9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0D05676p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0D05676g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell envelope</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552969.1">
        <property type="nucleotide sequence ID" value="XM_002552923.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGI9"/>
    <dbReference type="STRING" id="559295.C5DGI9"/>
    <dbReference type="GeneID" id="8295197"/>
    <dbReference type="KEGG" id="lth:KLTH0D05676g"/>
    <dbReference type="eggNOG" id="ENOG502S09G">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054077_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGI9"/>
    <dbReference type="OMA" id="QTITSCE"/>
    <dbReference type="OrthoDB" id="5415592at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005199">
        <property type="term" value="F:structural constituent of cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000420">
        <property type="entry name" value="Yeast_PIR_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00399">
        <property type="entry name" value="PIR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00929">
        <property type="entry name" value="PIR_REPEAT_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50256">
        <property type="entry name" value="PIR_REPEAT_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950470" evidence="4">
        <location>
            <begin position="21"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="141"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="149"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR22531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="335" mass="32487" checksum="B40830D64128B239" modified="2009-07-28" version="1">MKFTSAFTAALLACARLALADSETFGLLVIRSGSSLQYASAYAQDSKLYLGSSSDSLSAVVTDDGKLKLSDDKYAVVSSDGSVVEGSESEGSSEFSISEGHLLYGGKDGFVAVPSGSAYLLYTNNSDSSSIGVAIRATSTSGSTVPDFSPSGEDSSSAGSSSTAASSGTSTASQTTLSPSSGADAAVSQIGDGQIQATTATSSKAASSAAAVSQIGDGQIQATTATSSKLTSSAAAVSQIGDGQIQATTATSSKPTSSAAAVSQIGDGQIQAATSVASPSSSSSKTTVSAVSQISDGQIQATSSSASVQVQSENGGAQAVVGLGAGFAAAAALLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGJ1</accession>
    <name>C5DGJ1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0D05720p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0D05720g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552971.1">
        <property type="nucleotide sequence ID" value="XM_002552925.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGJ1"/>
    <dbReference type="FunCoup" id="C5DGJ1">
        <property type="interactions" value="770"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGJ1"/>
    <dbReference type="GeneID" id="8295199"/>
    <dbReference type="KEGG" id="lth:KLTH0D05720g"/>
    <dbReference type="eggNOG" id="KOG2989">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053603_2_1_1"/>
    <dbReference type="InParanoid" id="C5DGJ1"/>
    <dbReference type="OMA" id="NNDYQCE"/>
    <dbReference type="OrthoDB" id="674963at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071006">
        <property type="term" value="C:U2-type catalytic step 1 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007590">
        <property type="entry name" value="Saf4/Yju2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12111">
        <property type="entry name" value="SPLICING FACTOR YJU2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12111:SF1">
        <property type="entry name" value="SPLICING FACTOR YJU2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04502">
        <property type="entry name" value="Saf4_Yju2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="191"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="248"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="191"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="263"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="272" mass="31412" checksum="6871291F036448D1" modified="2009-07-28" version="1">MSERKAINKYYPPNFNPLEAEEAARKLSKKLKTMNRDVITIRLMTPFSMRCLKCSEFIPKSRKFNGKKELLPEKYLDTIKQYRLSIKCPRCNNMIAFRTDPKSGDYVMEIGGVKNFVEEKPEEKLPGDETLDETLERLERQQQQDNNQTKNDDHEDKLEKIEKRLAKLQKEQQDYEELEALRRENISKMKKAEALQEKSHLSAQEESQGDEEKAELAFRLFNAQKADGETSGPAVHITAAPAAIPQKIRSKKKAKSNALGVIVKKKNRARQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGN5</accession>
    <name>C5DGN5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">KLTH0D06754p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0D06754g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oligopeptide OPT transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553015.1">
        <property type="nucleotide sequence ID" value="XM_002552969.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGN5"/>
    <dbReference type="FunCoup" id="C5DGN5">
        <property type="interactions" value="125"/>
    </dbReference>
    <dbReference type="GeneID" id="8295246"/>
    <dbReference type="KEGG" id="lth:KLTH0D06754g"/>
    <dbReference type="eggNOG" id="KOG2262">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004965_1_1_1"/>
    <dbReference type="InParanoid" id="C5DGN5"/>
    <dbReference type="OMA" id="RWIVYPA"/>
    <dbReference type="OrthoDB" id="9986677at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035673">
        <property type="term" value="F:oligopeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004648">
        <property type="entry name" value="Oligpept_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004813">
        <property type="entry name" value="OPT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00727">
        <property type="entry name" value="ISP4_OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00728">
        <property type="entry name" value="OPT_sfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22601">
        <property type="entry name" value="ISP4 LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03169">
        <property type="entry name" value="OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0571">Peptide transport</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="167"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="206"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="239"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="380"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="450"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="511"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="538"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="577"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="618"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="685"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="763"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR22577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="827" mass="93928" checksum="21118AF298E206FE" modified="2009-07-28" version="1">MLYKEATSSTEAAMHRSSEDKSPAHNSNHLKMGKEVKIVDTLQVVEHGENSSDSSSLGQELSPGGSHVTYLSRGHLANEGAGAEGKTEQEIIEEEELTEQTVWEGNVSLLQNSPFPEVRATVPPTDDPTIVLNHWRTWFLTTVFVIVFAGVNQFFSLRYPSLSINFLVAQVVCYPVGKILALLPEWDCPRCPWFNLNPGPFTKKEHAVVTIAVALTSSTAYAMNILIAQTNFYNMKLSAGYMILLVWTSQMLVYGAAGLTRRWVVSPAACIWPQTLISVSLFDSLHNRSIDKTKVNGWSMSRYRFFVTVFSASFVWYWVPGFLFKGLSYFNVVLWGPKTRHNFIANTIFGVESGIGALPITFDYTQISQALTGSVFATPYWVSANSCASAVLFFVIILPILYFTNTWYAKYMPVISGSTYDNTQQKYNVSRILNSDYSINFEKYKSYSPLFVPFSYLLSYALNFAAVIAVFVHCFLYNGKEIKNKFRNKNYGGQDIHMRLYSQNYKDCPDWWYVVLQVIMIALGFVAVCAFNSHLPAWAFVVALAISFVNFIPQGILEAVTNQHVGLNIITELICGYMLPFRPMANLLFKLYGFIVMRQGLELSRDLKLALYMKVPPRLIFAVQIYATVISGLVNVGIQEWMRSNIKELCSSTQPDGFTCNNGRTIFNASIIWSMPKYLFSPGRIYNPIMWFFLIGLVVPIIVFVLQKKFKGNEMLKFCHSPVFFAGPGNIPPSTPYNYTLYFALSWALNTIRKRFPRWFSKYNFVMGAGVETGVALAVVIIFLCVQYPGGSLNWWGNNVYKNTYDHNYRKFYNLKKGEKFGYDKWW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-04-02" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGN9</accession>
    <name>C5DGN9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">KLTH0D06842p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0D06842g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553019.1">
        <property type="nucleotide sequence ID" value="XM_002552973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGN9"/>
    <dbReference type="GeneID" id="8295250"/>
    <dbReference type="KEGG" id="lth:KLTH0D06842g"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_538686_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGN9"/>
    <dbReference type="OMA" id="VYIHEAQ"/>
    <dbReference type="OrthoDB" id="654211at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051574">
        <property type="entry name" value="ZnF_E-box_Homeobox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24391">
        <property type="entry name" value="HISTONE H4 TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24391:SF27">
        <property type="entry name" value="ZINC FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="388"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="96"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="237"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="31"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="48"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="110"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="237"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="283"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR22581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="506" mass="53773" checksum="4AEC9C2ED744FBE7" modified="2009-07-28" version="1">MLSVHPYTSPADIDRDARRGSSQLDFRRRSSASSVASIGGAAAGGGPAQSPTSALWAAAPAPAPAPPLVPPAAVSSTVHRQPQYSVAQMHSQAYAIRQKHARASSTDSSGTPIPGPAAAVTTPGPASVPAPVTAAQQQPPPPPIGGPLYELMEYDTRYLPYHYKSAYPMWGPAGPTAAAAGTAPGTAPGAPGAAYPAYQYPPPAWNSYQAPHHVWNGQPAASSGGYPHMQLQIPTQLPQQPQMTTSQQQQQQQQQQQQQPHQPQQPQQQYQFQQQPQQPQQPSLHRQSISHRQSQGFSADQFPSALAATELPLSAYAPDPRSGAQLAGRPYVVAAAPGPAAASAPFGLGAVGIGAGAGAGARGPAPYSGTGSDRGSFDDTGSSMAMLYRCSMCEKSFKRKSWHQRHLLSHSSFKPYNCPWCQSRHKRRDNLFQHMKTKHVHQVLQELMEAGDLDGSMGTNVRLAIDDMVAGPSIRTLVQDGRVKKERVKTVLNAVIARVHDGEPGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-04-02" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGR7</accession>
    <name>C5DGR7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0D07524p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0D07524g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553047.1">
        <property type="nucleotide sequence ID" value="XM_002553001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGR7"/>
    <dbReference type="GeneID" id="8295279"/>
    <dbReference type="KEGG" id="lth:KLTH0D07524g"/>
    <dbReference type="eggNOG" id="ENOG502RY8K">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_586688_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGR7"/>
    <dbReference type="OMA" id="AFVYYQL"/>
    <dbReference type="OrthoDB" id="5319015at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056196">
        <property type="entry name" value="Mmc1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38644">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38644:SF1">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23868">
        <property type="entry name" value="Mmc1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23867">
        <property type="entry name" value="Mmc1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mmc1 C-terminal" evidence="1">
        <location>
            <begin position="256"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="456" mass="52320" checksum="6BA693724D4AC9E1" modified="2009-07-28" version="1">MAWRLRLNGLGKRLSSNWSRLSEIYAKAGDKVPDKLPLLKNEANHKIRCGVILQSLKSQRQSAFLSALLSDVYSNDQYWHDQFSQRYSKRGPKLIRYGASLEVHRLDNSSTLYTLPSPWLQKHDIELLEMDEVSPANDGCHIYLGLGKALPAMVWTTLSISDTDSESPMGTREVNCKKALAGMRKLIQSKSNVTEYLKDLESSNFLYITQLLESQLCDKNHIMERLKHAVIKNIYETDTSISKYSQHLTEKKTIDGEIDNWAYKAHSALQSQLKPRLKQFVHGQLSVYKLHTYSESQLELKLREMCDLSRTSDLTEKINHIRGRLNLPLSSPPVWDMNRLHQRANAMHKVINKFIYQQFFKFQLPLILCSVLGVLSGQFSAYSMGSLAALGLVLGVNRTMHQWSAILQRFQTEVLEEKRLEIEVARGEIQTESNIKAENESRILRSKVEILEKLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGW0</accession>
    <name>C5DGW0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0D08712p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0D08712g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553090.1">
        <property type="nucleotide sequence ID" value="XM_002553044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGW0"/>
    <dbReference type="FunCoup" id="C5DGW0">
        <property type="interactions" value="297"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGW0"/>
    <dbReference type="GeneID" id="8295326"/>
    <dbReference type="KEGG" id="lth:KLTH0D08712g"/>
    <dbReference type="eggNOG" id="KOG0585">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019325_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGW0"/>
    <dbReference type="OMA" id="NQIMKWI"/>
    <dbReference type="OrthoDB" id="68483at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF71">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="80"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="451"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="473"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR22652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="604" mass="67185" checksum="C380DF2E673F7F79" modified="2009-07-28" version="1">MNEFEVEVSKSSNTQLPTLIDSKGYAEGGDQVLAQSGFTDKYAVSYHQIIEQERALLDKRLRPVVKNTVHSDSKRKVGEYTLVRRIGKGQFGDVYMANTADGGYVAIKCVPKRPRKSQQYSMNQVLRQIRRQQSQGRRSLSSDEAILEMNVNKIRWEIFVASGFNHKNILGVIECLDSRDSKDIWIVLPFASLGELKWKRSSKPETLEQWDLTLRHKTSVTEFSEYVLRSLTAGLLYLWDQGCIHRDIKPSNILIDGSTGNIVLSDFGSSLLSPKKLLLEEPAMQKAYSEEVKKIVGTPAFIAPELCNFEENDSSLDGFKIDLWSLGVTIFCLLENRLPFWGENEFDTFQKIANNELPLSGDWLHDLVVSKLLRKDPTHRLSIKQLDSLLRNPKKESGMKKLVSRLRKISMKKKKGAKVPEASAGKAAIAVPSNVETSDVEFSDIESNISSAASLSDEPVQVPGFMDCRPPTDNGSPSVTASELSSRATPLELSPQQPSPLPVTPKHTTHDTSPSPIKIDTPLKNLIRAENTPEKAPSLSPMPVIKPQAQVSQQASRLASKGTLDFTKYLQSPSKVPKDCAPVGSTKASNSIEDIKRYLNFAES</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>C5DH30</accession>
    <name>C5DH30_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0E00924p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0E00924g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553528.1">
        <property type="nucleotide sequence ID" value="XM_002553482.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DH30"/>
    <dbReference type="GeneID" id="8291666"/>
    <dbReference type="KEGG" id="lth:KLTH0E00924g"/>
    <dbReference type="HOGENOM" id="CLU_2004330_0_0_1"/>
    <dbReference type="InParanoid" id="C5DH30"/>
    <dbReference type="OrthoDB" id="10528660at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR23091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="124" mass="12973" checksum="68178786770F5958" modified="2009-07-28" version="1">MELIKGRQGLRQVLCHSHGVVAPATDTRTSPRAPQSSVGSDKGFEIGSDADFLCQCLLLFSAGGLQAQRDALVAQALKTVPFDVTSEEAVVAYASSCAFGAIGPFGPGGRRLGLRAQPDRSAQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>C5DH75</accession>
    <name>C5DH75_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0E01936p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0E01936g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553573.1">
        <property type="nucleotide sequence ID" value="XM_002553527.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DH75"/>
    <dbReference type="FunCoup" id="C5DH75">
        <property type="interactions" value="78"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DH75"/>
    <dbReference type="GeneID" id="8291712"/>
    <dbReference type="KEGG" id="lth:KLTH0E01936g"/>
    <dbReference type="HOGENOM" id="CLU_096885_0_0_1"/>
    <dbReference type="InParanoid" id="C5DH75"/>
    <dbReference type="OMA" id="QIHSGYT"/>
    <dbReference type="OrthoDB" id="4035717at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048744">
        <property type="entry name" value="MCM16"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20993">
        <property type="entry name" value="CENPH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="202" mass="23269" checksum="B4970C9A15AB78E5" modified="2009-07-28" version="1">MESRLQELEERHVSVYRELLTALDELYLLRKGIQPEDETLLVVRRKLQSSLTMTAPMMSAMNGKTALDKRLTRLMKENHELDAEVAAHQDEKLRLLRELSDERMRYKELVNNARELTERQAHAQGIGEQRMPRGRQDANKSTDSDLYDSSEQGDSTEAGSEQRDKAQIERENEALRELLVGMIVHGGYLGTNKVFDEWLGDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHG6</accession>
    <name>C5DHG6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Succinate dehydrogenase [ubiquinone] cytochrome b small subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0E04180g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Mitochondrion inner membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the CybS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553664.1">
        <property type="nucleotide sequence ID" value="XM_002553618.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHG6"/>
    <dbReference type="FunCoup" id="C5DHG6">
        <property type="interactions" value="85"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHG6"/>
    <dbReference type="GeneID" id="8291813"/>
    <dbReference type="KEGG" id="lth:KLTH0E04180g"/>
    <dbReference type="eggNOG" id="KOG4097">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096618_0_0_1"/>
    <dbReference type="InParanoid" id="C5DHG6"/>
    <dbReference type="OMA" id="DYIPKRK"/>
    <dbReference type="OrthoDB" id="18577at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042721">
        <property type="term" value="C:TIM22 mitochondrial import inner membrane insertion complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03496">
        <property type="entry name" value="SQR_TypeC_CybS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1300.10:FF:000007">
        <property type="entry name" value="Succinate dehydrogenase [ubiquinone] cytochrome b small subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1300.10">
        <property type="entry name" value="Fumarate reductase/succinate dehydrogenase, transmembrane subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007992">
        <property type="entry name" value="CybS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034804">
        <property type="entry name" value="SQR/QFR_C/D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13337">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13337:SF2">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE [UBIQUINONE] CYTOCHROME B SMALL SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05328">
        <property type="entry name" value="CybS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81343">
        <property type="entry name" value="Fumarate reductase respiratory complex transmembrane subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 12" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="104"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="142"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="11">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <note>ligand shared with SDHC</note>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>a ubiquinone</name>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <note>ligand shared with IP/SDHB</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR607992-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR607992-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU364031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR23227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="189" mass="21060" checksum="3025C3E9D1A6D9FD" modified="2009-07-28" version="1">MLKRCFESNLRSGTRSVLTRAKLSQHRPSIGLRPMSLKPNFSKLKIVPPPPGGVEGGVNDPLKPVEIDYFHGSYHWDYERITAVMLVPLTLVPMYGALSSGTLYPLLDAMLCSVLLIHSQLGITSCIIDYIPKRKFGIWHKLAMGMLYTGSAVGLYGVYELETSNNGLVDLIGRLWKEDESGLTIFSRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHN6</accession>
    <name>C5DHN6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0E05808p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0E05808g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the lipid-translocating exporter (LTE) (TC 9.A.26.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553734.1">
        <property type="nucleotide sequence ID" value="XM_002553688.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHN6"/>
    <dbReference type="FunCoup" id="C5DHN6">
        <property type="interactions" value="30"/>
    </dbReference>
    <dbReference type="GeneID" id="8291887"/>
    <dbReference type="KEGG" id="lth:KLTH0E05808g"/>
    <dbReference type="eggNOG" id="ENOG502QURG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033465_3_1_1"/>
    <dbReference type="InParanoid" id="C5DHN6"/>
    <dbReference type="OMA" id="RSHICAS"/>
    <dbReference type="OrthoDB" id="3358017at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007568">
        <property type="entry name" value="RTA1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31465">
        <property type="entry name" value="PROTEIN RTA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31465:SF1">
        <property type="entry name" value="PROTEIN RTA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04479">
        <property type="entry name" value="RTA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="107"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="147"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="181"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="224"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="262"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR23297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="310" mass="34535" checksum="C2C52EC4A93C08DE" modified="2009-07-28" version="1">MGTSTSDFELYNYTPSTSAAALFAAVFALSLLVLSVEVIVLQRRAKKTISVTMDDRLNHSEYSMVGLSRKKPSSKFVCLIVGCGLETIGYIARAYSSKNQDKILPYVIQSMFVLVAPALIAASIYMIFGAMLSLLDCSSLSIIPARFNTMFFVCGDIFSFLLQCGGGGLMAKSSSQKTGEILAIIGLIVQLVFFGFFLLTEIRFLILARGRADLTLKFGNKWKIINWTLIVSSIFILIRCIVRTAEFCQGREGYLMSHEWTIYVFDALFMTASVWVFAVSLPFASLFDLNMVKMAVNEFKVVPSEYESRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHR3</accession>
    <name>C5DHR3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">mRNA m(6)A methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.348</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0E06468g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>an adenosine in mRNA + S-adenosyl-L-methionine = an N(6)-methyladenosine in mRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:55584"/>
            <dbReference type="Rhea" id="RHEA-COMP:12414"/>
            <dbReference type="Rhea" id="RHEA-COMP:12417"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74449"/>
            <dbReference type="EC" id="2.1.1.348"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the MT-A70-like family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.348" evidence="1"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553761.1">
        <property type="nucleotide sequence ID" value="XM_002553715.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHR3"/>
    <dbReference type="FunCoup" id="C5DHR3">
        <property type="interactions" value="37"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHR3"/>
    <dbReference type="GeneID" id="8291915"/>
    <dbReference type="KEGG" id="lth:KLTH0E06468g"/>
    <dbReference type="eggNOG" id="KOG2098">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018702_4_1_1"/>
    <dbReference type="InParanoid" id="C5DHR3"/>
    <dbReference type="OMA" id="WIRCDVR"/>
    <dbReference type="OrthoDB" id="10262526at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036396">
        <property type="term" value="C:RNA N6-methyladenosine methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001734">
        <property type="term" value="F:mRNA m(6)A methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007757">
        <property type="entry name" value="MT-A70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12829">
        <property type="entry name" value="N6-ADENOSINE-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12829:SF7">
        <property type="entry name" value="N6-ADENOSINE-METHYLTRANSFERASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05063">
        <property type="entry name" value="MT-A70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51143">
        <property type="entry name" value="MT_A70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR23324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="522" mass="58391" checksum="D931C5EF430FFCC3" modified="2009-07-28" version="1">MNMQLITYLIENHAALLNPPISGDLANIYILFASTSKHESAGMKQQEAKLAAFWKELKDIADLCPGCFIFASTGYGSAALSSRDVTVEYIDIQALRMIRDSMISTPAEVARPVRPLGRESSEELALSDRLLSVLDSDAAATPNKRVLAKLEHFLSVKPASYRLGHERAQKFTPHSKFIPICGNRSHTNALASIASIAGNRVLSLDWNQLAGADQSTPGLSRCVQSKIHHIPNLKPQTDKTLGDCSYLDTCHKLSTCRYMHYLQYVPESLVQSVAARSRELNDAESDSKSICLYTRNLCCSVASKSVLPAQWIQCDVRKFDFSVLGQFSVVVADPAWNIRMNLPYGTCNDSELTDLPLELLQREGVLFLWVTGRAIEVGKESLRKWGYEVCNQISWVKTNQLGRTIVTGRTGHWLNHSKEQLLVGLKGQPEWLNRQCDVDLIVSGSRETSRKPDELYEMIERMVGPHARKLEIFGRDHNTRPGWFTIGNQLTGDSLCEPDVQRKYRQFLHTQRTSYGCSSVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHS7</accession>
    <name>C5DHS7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0E06798p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0E06798g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553775.1">
        <property type="nucleotide sequence ID" value="XM_002553729.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHS7"/>
    <dbReference type="FunCoup" id="C5DHS7">
        <property type="interactions" value="25"/>
    </dbReference>
    <dbReference type="GeneID" id="8291930"/>
    <dbReference type="KEGG" id="lth:KLTH0E06798g"/>
    <dbReference type="eggNOG" id="ENOG502RW3H">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088285_2_1_1"/>
    <dbReference type="InParanoid" id="C5DHS7"/>
    <dbReference type="OMA" id="SGPDWFY"/>
    <dbReference type="OrthoDB" id="2158714at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013640">
        <property type="entry name" value="Vfa1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28218">
        <property type="entry name" value="VPS4-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28218:SF1">
        <property type="entry name" value="VPS4-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08432">
        <property type="entry name" value="Vfa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="170"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="170"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="206" mass="23500" checksum="D8138ACE8C13A9E0" modified="2009-07-28" version="1">MQNQYTKRKVAPRDARSCLVCLKPTACVLYNASIQDWFHCCELHLQDNPQFAKPVYPAGYQDALGQLAELKRTLDAQRVHGSGSWDTWVNKLISRKPKSENDKDKEPGKEPSKDPDEEPGKGSDKEPSPAGDDAACAYQQALDRVARYRKNLRTYSLSQIMFESRLEAQKRAEALKQRRQREQEAYTNTDPDELQAKFAFPSVPRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHT0</accession>
    <name>C5DHT0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0E06864p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0E06864g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553778.1">
        <property type="nucleotide sequence ID" value="XM_002553732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHT0"/>
    <dbReference type="FunCoup" id="C5DHT0">
        <property type="interactions" value="98"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHT0"/>
    <dbReference type="GeneID" id="8291933"/>
    <dbReference type="KEGG" id="lth:KLTH0E06864g"/>
    <dbReference type="eggNOG" id="KOG2049">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017863_0_0_1"/>
    <dbReference type="InParanoid" id="C5DHT0"/>
    <dbReference type="OMA" id="PPLGQMN"/>
    <dbReference type="OrthoDB" id="668540at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010629">
        <property type="term" value="P:negative regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010608">
        <property type="term" value="P:post-transcriptional regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080090">
        <property type="term" value="P:regulation of primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07920">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033133">
        <property type="entry name" value="PUM-HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033712">
        <property type="entry name" value="Pumilio_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537">
        <property type="entry name" value="RNA BINDING PROTEIN PUMILIO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537:SF80">
        <property type="entry name" value="SUPPRESSOR PROTEIN MPT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00806">
        <property type="entry name" value="PUF"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50303">
        <property type="entry name" value="PUM_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PUM-HD" evidence="4">
        <location>
            <begin position="139"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="206"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="242"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="321"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="47"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="106"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="594"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="57"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="67"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="113"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR23341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="732" mass="80296" checksum="DA02B531610B650E" modified="2009-07-28" version="1">MHQPQLSINSVQSLVEPTTPPLGQMTQRQNHQRTHSLDLSGFNQFMQGNSPMMPLSLSPPTPPPPAAAAAASASASATTAGRPKLQSLPLISELDLLADGRGPRAPIPTGAPARTVSGSTTSSSASASAVRTHARSGSQPSALLLPNAGAAGAAGATGVPADLSWTPLDELDYVKVATDQFGCRFLQKKLENPQECCAVRDLMYRHIKPYFLDLILDPFGNYLIQKLCEYLTTDQKTQLVESIYPHVFQISINQYGTRSLQKVIDTVDTDAQVDLIISGFGQNFTSIEQVVVLINDLNGNHVVQKCIFKFPPSKFGFIIDAIVDNNNIVKISTHKHGCCVLQKLLSVCTLQQIFKISVKIVQFLRSLINDQFGNYIIQFLLDIKELDFYLLGEIYDKLSGELCQLSCLKFSSNVVEKFIKKLFTILKLALSAAPPARNSAVSEDVVAIAMKILLNVIDVFTVNLNILIRDNYGNYALQTLLDVKNYTKLLQYPSNTYVHTSAQTLSFSHEFTVKVGNLVVLTKEMLPSIKTTSYAKKIKLKVKAYAELTGLVFAEAPSSNKAATPGANANNHRRQHARHCSLPANAYRHRRNSSSISLSSGFPLPQPPQQQQHQPFGYANPLVNSSLFANANATQLGPHNFYSDPQRPFLYNQHPEASLPFNNSFGNAPSAAVPPNHFAYHDPRVLQMSGAQENNHNFAPTAKPLPSKPAETENLYARGGDFENVNYGNIFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHY7</accession>
    <name>C5DHY7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">KLTH0E08206p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0E08206g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553835.1">
        <property type="nucleotide sequence ID" value="XM_002553789.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHY7"/>
    <dbReference type="FunCoup" id="C5DHY7">
        <property type="interactions" value="441"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHY7"/>
    <dbReference type="GeneID" id="8291994"/>
    <dbReference type="KEGG" id="lth:KLTH0E08206g"/>
    <dbReference type="eggNOG" id="KOG1690">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066963_2_1_1"/>
    <dbReference type="InParanoid" id="C5DHY7"/>
    <dbReference type="OMA" id="GATCAWQ"/>
    <dbReference type="OrthoDB" id="3427at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015720">
        <property type="entry name" value="Emp24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
        <property type="entry name" value="TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002947849" evidence="10">
        <location>
            <begin position="23"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="11">
        <location>
            <begin position="32"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="145"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR23398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="219" mass="25281" checksum="E0D54C578A41E71D" modified="2009-07-28" version="1">MKLFTCLLQLSALLLLTPFANAFYYYSSGGERRCFHKELPKNTLLQGKYSVQTYDDKAKAYVTPSRDQLSVVVEVEEIFDDNHRVMNQKLAAQSEFTFNAHDSGEHKICIQPQSTGWLAKVKTKVDIEFQTGSDASLDSKGKNTMQSLQSKIKVLNDRVEEIRREQELVREREAMFRDVSESANSRAMWWTVFQVVVLAGTCAWQLRHLRTFFVKQKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>C5DI15</accession>
    <name>C5DI15_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0E08888p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0E08888g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553863.1">
        <property type="nucleotide sequence ID" value="XM_002553817.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DI15"/>
    <dbReference type="FunCoup" id="C5DI15">
        <property type="interactions" value="53"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DI15"/>
    <dbReference type="GeneID" id="8292024"/>
    <dbReference type="KEGG" id="lth:KLTH0E08888g"/>
    <dbReference type="eggNOG" id="ENOG502S3VI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119636_0_0_1"/>
    <dbReference type="InParanoid" id="C5DI15"/>
    <dbReference type="OMA" id="CINIRYD"/>
    <dbReference type="OrthoDB" id="4074030at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36784">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36784:SF1">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="148"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="191" mass="21931" checksum="F834D187AEB1E3EF" modified="2009-07-28" version="1">MSRVRKFNRGVLSKIHSDSDTDEDIPLHPLDIEEQEELISKLEFRNVSSNNYNIQLLSVAYVVCCGVFLSLVMKVQKIRGDTSMNKRLLLYSVNSIICSLLNLRYDLTKDFALSRAVKVRITSRKINIINCILLLLLTWEVTGKVDKILLQLLFHVPLLLFSLSVVSKKWICDLDEELNGLRGLKYKFKSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>C5DI51</accession>
    <name>C5DI51_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0E09746p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0E09746g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553899.1">
        <property type="nucleotide sequence ID" value="XM_002553853.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DI51"/>
    <dbReference type="FunCoup" id="C5DI51">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="GeneID" id="8292063"/>
    <dbReference type="KEGG" id="lth:KLTH0E09746g"/>
    <dbReference type="eggNOG" id="ENOG502S6F5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1034658_0_0_1"/>
    <dbReference type="InParanoid" id="C5DI51"/>
    <dbReference type="OMA" id="HFARINY"/>
    <dbReference type="OrthoDB" id="4064753at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR23462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="269" mass="31597" checksum="44038A9DCC402C41" modified="2009-07-28" version="1">MRISLSLNAFVSPISVTSRKSFEKLDKTPSTVFQYYYQLQLKSLNRSGLPADILEPVNTGHNSLRRYVRKEWESLSMSKKRVYHALYFHFIKLNYKELAPEEFAKRLELPIPISSEYLLFRNKFKLEFDRIWKEQCKKDHHGRSRRIYLRTGPSNLLKASRTIKGTESGFDYDSTRQFQEMCRQCRKVWRDKVTSDQKAQMVEILQKNRQEFKLKLEQEVKILDSLQNALQKYLAGPSAKISTALDFERPVTQSHHSVIALLLATGKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5DID6</accession>
    <name>C5DID6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pre-mRNA-splicing factor CWC21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0E11682g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CWC21 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553984.1">
        <property type="nucleotide sequence ID" value="XM_002553938.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DID6"/>
    <dbReference type="FunCoup" id="C5DID6">
        <property type="interactions" value="79"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DID6"/>
    <dbReference type="GeneID" id="8292151"/>
    <dbReference type="KEGG" id="lth:KLTH0E11682g"/>
    <dbReference type="eggNOG" id="KOG1869">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067891_3_1_1"/>
    <dbReference type="InParanoid" id="C5DID6"/>
    <dbReference type="OMA" id="RIEVKCM"/>
    <dbReference type="OrthoDB" id="10267305at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21372">
        <property type="entry name" value="cwf21_CWC21-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051372">
        <property type="entry name" value="CWC21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013170">
        <property type="entry name" value="mRNA_splic_Cwf21_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36562">
        <property type="entry name" value="SERINE/ARGININE REPETITIVE MATRIX 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36562:SF5">
        <property type="entry name" value="SERINE_ARGININE REPETITIVE MATRIX 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08312">
        <property type="entry name" value="cwf21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01115">
        <property type="entry name" value="cwf21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="CWF21" evidence="9">
        <location>
            <begin position="61"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="12"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR23547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="134" mass="15252" checksum="D0980F5407BC5A0E" modified="2009-07-28" version="1">MSYDGIGLKSAKGSSTSGHIQQSLALNTERKNVKNFLSRVEKQQQRPKSSAQTKRKDESILKHLSKRELELRVSEYRDALEDDDSLSDATIDAKCQEFREKTALQLRKEHVDEKLRNAYVSRSKRQAESGAADQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIJ3</accession>
    <name>C5DIJ3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0E12980p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0E12980g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SEN54 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554041.1">
        <property type="nucleotide sequence ID" value="XM_002553995.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIJ3"/>
    <dbReference type="FunCoup" id="C5DIJ3">
        <property type="interactions" value="74"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIJ3"/>
    <dbReference type="GeneID" id="8292210"/>
    <dbReference type="KEGG" id="lth:KLTH0E12980g"/>
    <dbReference type="eggNOG" id="KOG4772">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028449_0_1_1"/>
    <dbReference type="InParanoid" id="C5DIJ3"/>
    <dbReference type="OMA" id="FNVWKPQ"/>
    <dbReference type="OrthoDB" id="408683at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000214">
        <property type="term" value="C:tRNA-intron endonuclease complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000379">
        <property type="term" value="P:tRNA-type intron splice site recognition and cleavage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024337">
        <property type="entry name" value="tRNA_splic_suSen54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024336">
        <property type="entry name" value="tRNA_splic_suSen54_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21027">
        <property type="entry name" value="TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21027:SF1">
        <property type="entry name" value="TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12928">
        <property type="entry name" value="tRNA_int_end_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA-splicing endonuclease subunit Sen54 N-terminal" evidence="4">
        <location>
            <begin position="73"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="349"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="417"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="20"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="361"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="423"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR23604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="443" mass="50490" checksum="1F9A0DB72A028913" modified="2009-07-28" version="1">MSSDMNELDEESFIPSSLLDSDDEADELPQDWSHIAKLSKGQVVVPKRGEKDYEPDGTNAQELLLHKAKTVMFDSLSDASRGSVVKNLIKAYYVPDLHKACVPHPKGSFMHTMGKVDRDGACWLSFFEFVYLAERGTIAPYYKRKESEDHALDPFLGIQDLYMLFKSQKESDEFAVYAHLKRLGFIVMQSLQNSAEVGSVGQFEDSAVQKLRLKFCQTFESLSKSISNPFTLPFYHPLQYHLTRYCTSAQIYESLNKLIPYAAAPKTLGELQKDLRMENVSAGIWKFSFDVWKPQSNFRKKTPGLPDFQVVVFNKNSHKENFPTYHEMRGLLKMLNCKMDFLNAPANEQAKSAETEANKGATKKKSKANKKYPPHIEQQRRIKRGYRSFVLATMDDGLVSFIRITETDFGSENVWFKPNNVPLRKEKSGPKASKHSEEAKASK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIJ7</accession>
    <name>C5DIJ7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 15">Elongator complex protein 3</fullName>
            <ecNumber evidence="15">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ordered locus">KLTH0E13068g</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalytic tRNA acetyltransferase subunit of the elongator complex, which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine). In the elongator complex, acts as a tRNA uridine(34) acetyltransferase by mediating formation of carboxymethyluridine in the wobble base at position 34 in tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>uridine(34) in tRNA + acetyl-CoA + S-adenosyl-L-methionine + H2O = 5-(carboxymethyl)uridine(34) in tRNA + 5'-deoxyadenosine + L-methionine + CoA + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:61020"/>
            <dbReference type="Rhea" id="RHEA-COMP:10407"/>
            <dbReference type="Rhea" id="RHEA-COMP:11727"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74882"/>
            <dbReference type="EC" id="2.3.1.311"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:61021"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 16">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="15 16">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the ELP3 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="15"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554045.1">
        <property type="nucleotide sequence ID" value="XM_002553999.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIJ7"/>
    <dbReference type="FunCoup" id="C5DIJ7">
        <property type="interactions" value="1112"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIJ7"/>
    <dbReference type="GeneID" id="8292214"/>
    <dbReference type="KEGG" id="lth:KLTH0E13068g"/>
    <dbReference type="eggNOG" id="KOG2535">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025983_2_1_1"/>
    <dbReference type="InParanoid" id="C5DIJ7"/>
    <dbReference type="OMA" id="TFETRPD"/>
    <dbReference type="OrthoDB" id="10265243at2759"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033588">
        <property type="term" value="C:elongator holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106261">
        <property type="term" value="F:tRNA uridine(34) acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002926">
        <property type="term" value="P:tRNA wobble base 5-methoxycarbonylmethyl-2-thiouridinylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000003">
        <property type="entry name" value="Elongator complex protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039661">
        <property type="entry name" value="ELP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034687">
        <property type="entry name" value="ELP3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056591">
        <property type="entry name" value="ELP3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032432">
        <property type="entry name" value="Radical_SAM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01211">
        <property type="entry name" value="ELP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135:SF0">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23613">
        <property type="entry name" value="ELP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16199">
        <property type="entry name" value="Radical_SAM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005669">
        <property type="entry name" value="Hist_AcTrfase_ELP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01086">
        <property type="entry name" value="elongater_protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00344">
        <property type="entry name" value="ELP3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="13 15" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="11 16" id="KW-0408">Iron</keyword>
    <keyword evidence="12 15 16" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 15 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 15" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 15" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="5 15" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Radical SAM core" evidence="18">
        <location>
            <begin position="89"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="17">
        <location>
            <begin position="403"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR005669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR005669-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAR23608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="555" mass="63377" checksum="8FDA1AB156F8BD2B" modified="2009-07-28" version="1">MARRGPKTNKQKLAPEKERFIQCCSDITLELTNSLTSGSTKETNLNGLITKFSKKYKLKQQPRLTDIINSIPDQYKKYLLPKLKAKPVRTASGIAVVAVMCKPHRCPHIAYTGNICVYCPGGPDSDFEYSTQSYTGYEPTSMRAIRARYDPYEQARGRVEQLKQLGHSIDKVEYIIMGGTFMSLPKDYREDFIVKLHNALSGFNGNDIDEAIQFSQQSLTKCVGITIETRPDYCTHTHLDDMLKYGCTRLEIGVQSLYEDVARDTNRGHTVKSVAETFCVAKDAGYKIVSHMMPDLPNVGMERDIEQFKEYFENPAFRTDGLKIYPTLVIRGTGLYELWKTGRYKSYNANALVDLVARIMALVPPWTRIYRVQRDIPMPLVTSGVDNGNLRELALARMKDFGTTCRDVRTREVGIQEVHHKVQPDQVELIRRDYYANGGWETFLSYEDPKQDILIGLLRLRKASKKYTYRKEFTSQRTSIVRELHVYGSVVPLHSRDPRKFQHQGFGTLLMEEAARIAKEEHGSEKISVISGVGVRNYYAKLGYELDGPYMSKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIQ4</accession>
    <name>C5DIQ4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0E14344p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0E14344g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TEC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554102.1">
        <property type="nucleotide sequence ID" value="XM_002554056.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIQ4"/>
    <dbReference type="FunCoup" id="C5DIQ4">
        <property type="interactions" value="2543"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIQ4"/>
    <dbReference type="GeneID" id="8292272"/>
    <dbReference type="KEGG" id="lth:KLTH0E14344g"/>
    <dbReference type="eggNOG" id="KOG3841">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_508106_0_0_1"/>
    <dbReference type="InParanoid" id="C5DIQ4"/>
    <dbReference type="OrthoDB" id="10006572at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.20.40">
        <property type="entry name" value="TEA/ATTS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000818">
        <property type="entry name" value="TEA/ATTS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038096">
        <property type="entry name" value="TEA/ATTS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050937">
        <property type="entry name" value="TEC1_TEAD_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11834:SF0">
        <property type="entry name" value="PROTEIN SCALLOPED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11834">
        <property type="entry name" value="TRANSCRIPTIONAL ENHANCER FACTOR TEF RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01285">
        <property type="entry name" value="TEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00065">
        <property type="entry name" value="TEADOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00426">
        <property type="entry name" value="TEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51088">
        <property type="entry name" value="TEA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TEA" evidence="8">
        <location>
            <begin position="180"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="TEA" evidence="6">
        <location>
            <begin position="180"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="301"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="477"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="18"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="301"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR23665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="536" mass="56985" checksum="4178D32967EC9E7B" modified="2009-07-28" version="1">MNPDPNFGPPAGAGGGNHNSNNNSNSNSAGGSAGAVNTRIFDVYAGDASMPPHMMDVLACDAVAGGAATAGAGVGGLVLDDEEDTDSADEYEYERRGARRGLGAAGRAGPVPLINTGRGQIQVDVPSDPAAAARHVHNRPARLDLAPRDFAEQPLPHAQPQQLPQPLPHAQLPLQATQPPLTNTDIWSVPVENAFLAALRIILKKGTAKIKLKDRNYGRNELISLFIHHRVGEYRAKKQISSHIQVWKKSILNKLQNGSQITDYERELLDLIEHGPPQTPENERAFHAVFSEILDGPLAASRHSSSVSTSSSASPSNIPMPLHDAMISPHGAPAHGAGSALMAGAPPAGALPHGIPHITMYDPRIPSAAIMSAPPAYFPCTGAGPGLAQLPGSLPGSAATEPLTPLEYARRVYGKLRSFKCVPVNMHDYTYPYTDAPSAPLVHSDQQTLQAAQEVAAQQRRLIQNLYSSQHIYGPVPAPDFGAEQPEYSGYDRHSFVPPNAAQPPEQPLRRRSMSREDTPQDEGAGPGAQNPTDES</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIU7</accession>
    <name>C5DIU7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0E15312p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0E15312g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554145.1">
        <property type="nucleotide sequence ID" value="XM_002554099.1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="STRING" id="559295.C5DIU7"/>
    <dbReference type="GeneID" id="8292316"/>
    <dbReference type="KEGG" id="lth:KLTH0E15312g"/>
    <dbReference type="eggNOG" id="ENOG502S6JB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_129456_1_0_1"/>
    <dbReference type="InParanoid" id="C5DIU7"/>
    <dbReference type="OMA" id="RNKFIGW"/>
    <dbReference type="OrthoDB" id="284718at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28038">
        <property type="entry name" value="ADL329WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28038:SF1">
        <property type="entry name" value="ADL329WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR23708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="108" mass="11379" checksum="92460D494EA1E09C" modified="2009-07-28" version="1">MNNCKRPDLAVAYTHVPAPKSSAAAASQGIISQSMPMAAMFMKNKFLAWFGLLSSVHYCLTADAESSGDQSPVLKLVMALVSLGVCYMNLVFPQPGPADVAKAKAQSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-04-02" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJ19</accession>
    <name>C5DJ19_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0F12782p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0F12782g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554745.1">
        <property type="nucleotide sequence ID" value="XM_002554699.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJ19"/>
    <dbReference type="GeneID" id="8292968"/>
    <dbReference type="KEGG" id="lth:KLTH0F12782g"/>
    <dbReference type="eggNOG" id="KOG4476">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_692744_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJ19"/>
    <dbReference type="OMA" id="GLTRKWH"/>
    <dbReference type="OrthoDB" id="5572844at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018608">
        <property type="entry name" value="Gti1/Pac2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28027">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR MIT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28027:SF2">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR MIT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09729">
        <property type="entry name" value="Gti1_Pac2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="246"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="246"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="398" mass="43310" checksum="C3A1D8B3DC66C8F9" modified="2009-07-28" version="1">MNHTLVKVRPTFHGYIDSTTDVLLVLQAVLDGKLAPVERRPYEIERSQLIVSGNVFVFIEELSRIRRWTDGVRWSPSRILGKFLVYRELDKHRRGGPAPLRPNGLIKKALSLHLYDPGSHQRQTFHLISYYSKRDLHAHALSTPSHDPFFEYVRPCSQLVDALENTTIGGASRAATAATTAATAGSQGSSPASSTGTPKSATASVSVPASASVPASATDASCSAGARAPAPPLASLSAPALHAHLPSPLPGGLHPPQSYYYQQPQSASSSQASRTQYPFALPNQRQTLLPPPRLGRPRIQLPPLVNSAIPPPFPVHPSNIQLHALPPYQPMHTQPRAPPNFSDYRPPAFQQQHMHVPLPPHQTSCPPFLGSDLPPVAHITSQFQTDRTTTSPNFHFIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJ46</accession>
    <name>C5DJ46_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0F13420p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0F13420g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554772.1">
        <property type="nucleotide sequence ID" value="XM_002554726.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJ46"/>
    <dbReference type="FunCoup" id="C5DJ46">
        <property type="interactions" value="20"/>
    </dbReference>
    <dbReference type="GeneID" id="8292996"/>
    <dbReference type="KEGG" id="lth:KLTH0F13420g"/>
    <dbReference type="eggNOG" id="ENOG502RZ5Y">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072626_0_1_1"/>
    <dbReference type="InParanoid" id="C5DJ46"/>
    <dbReference type="OMA" id="WINPGDY"/>
    <dbReference type="OrthoDB" id="1476984at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047443">
        <property type="term" value="F:4-hydroxy-4-methyl-2-oxoglutarate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008948">
        <property type="term" value="F:oxaloacetate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16841">
        <property type="entry name" value="RraA_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.40">
        <property type="entry name" value="Ribonuclease E inhibitor RraA/RraA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005493">
        <property type="entry name" value="RraA/RraA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036704">
        <property type="entry name" value="RraA/RraA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33254:SF28">
        <property type="entry name" value="4-HYDROXY-4-METHYL-2-OXOGLUTARATE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33254">
        <property type="entry name" value="4-HYDROXY-4-METHYL-2-OXOGLUTARATE ALDOLASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03737">
        <property type="entry name" value="RraA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89562">
        <property type="entry name" value="RraA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="101"/>
            <end position="104"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR605493-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="235" mass="25920" checksum="CCF04AC5BB3D2FFC" modified="2009-07-28" version="1">MTSVSTKLSRFSTCDVSDGLLNRNGDPHGGFFPNLQKWSGRSEHSVAGKAYTVLFAPCDDPREEVNYIDSVPPGSFIVVALTIPLQLECAPYTQISQALYGGLMSTRAQYRKACGSIIFGRIRDLEEHRALGFPVFSYGLGSCAARMAVKPVGVNVPLQILSHRDEFRTINPGDYIVGDCHGIVRIPAAEDSEELVKYIEKSVEVDQYIADDVKNGKPLKPSQKERRAVLKNLTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJ59</accession>
    <name>C5DJ59_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Proteasome subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0F13750g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the proteasome, a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the proteasome complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the peptidase T1B family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554785.1">
        <property type="nucleotide sequence ID" value="XM_002554739.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJ59"/>
    <dbReference type="FunCoup" id="C5DJ59">
        <property type="interactions" value="1127"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJ59"/>
    <dbReference type="MEROPS" id="T01.984"/>
    <dbReference type="GeneID" id="8293011"/>
    <dbReference type="KEGG" id="lth:KLTH0F13750g"/>
    <dbReference type="eggNOG" id="KOG0177">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035750_12_0_1"/>
    <dbReference type="InParanoid" id="C5DJ59"/>
    <dbReference type="OMA" id="MKRDHDK"/>
    <dbReference type="OrthoDB" id="268428at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010499">
        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03758">
        <property type="entry name" value="proteasome_beta_type_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000008">
        <property type="entry name" value="Proteasome subunit beta type-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035206">
        <property type="entry name" value="Proteasome_beta2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF2">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA TYPE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2 5" id="KW-0647">Proteasome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00026071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR24348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="195" mass="22219" checksum="C7F5D2A5F8F48239" modified="2009-07-28" version="1">MDIILGIRVQNTVILATSKAVTRGISVLKADDDKTRQLSEHVLMAYSGEAGDAVQFSEYIQANIQLYSIRENYELSPKAVSSFVRQELAKSIRSRRPYQVNVLIGGYDAHQKKPELYQIDYLGTKVELPYAAHGYSGFYTFSLLDHHYRPDMTVEEGLKLLKLCIEELQRRMPVDFKGVLVKVVDENGVREVEDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJ63</accession>
    <name>C5DJ63_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0F13838p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0F13838g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554789.1">
        <property type="nucleotide sequence ID" value="XM_002554743.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJ63"/>
    <dbReference type="FunCoup" id="C5DJ63">
        <property type="interactions" value="317"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJ63"/>
    <dbReference type="GeneID" id="8293015"/>
    <dbReference type="KEGG" id="lth:KLTH0F13838g"/>
    <dbReference type="eggNOG" id="KOG1256">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_45_4_1"/>
    <dbReference type="InParanoid" id="C5DJ63"/>
    <dbReference type="OMA" id="WYHSIGL"/>
    <dbReference type="OrthoDB" id="1700726at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272:SF33">
        <property type="entry name" value="AMP-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="3">
        <location>
            <begin position="120"/>
            <end position="558"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR24352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="748" mass="83758" checksum="5859683BF6356472" modified="2009-07-28" version="1">MSKQDGYISLSELIETDKRFQNLREELAVYDKNSKEYLSNLISKLPLTNHVSYRQFLKEQAVSLESSKKHGYSPVFRSSLSPECLVSNVHPRLSTFFELFNFSVERFPDNDCLGQRSQDRVTGHWGQHYEFESYREIQERSQNLGSGIMTVVNLKRKRRFGSNDFIVSFLSTNRKEWVISDLACQGYSLGNTALYETLGLDTSEYILNVTESPVLILSKENIYRVMEMVPKLPHLSTIVCMDELSDLELAQLNGPLLPQHTNSKGERISILNFRQVERIGASNKVPLIPPTPDSLYTISFTSGTTGTPKGVQMKQSHVAAAVAFVLSTLRMPRLKHRSQAYDLCFLPLAHIFERQIVAFDLSSGTAIGFLHKPDPSVLVEDLKLLKPDVFPSVPRILTKFEAGIKNSLQNGDGSAVTKNVASTILNKRLERTTHHGGKDHSILNTVVFHRVLIDKIRSSLGLENLDVVITGSAPISNDTLLFMKSALDCGVRQGYGLTETFAGICLSEARERDSGTCGGMAVTTECRLRSIPEMGYDAEHDLKGEVQLRGSQVFRGYYKNPQETSRALGEDGWYSTGDVGFIDSKGRLSIIDRVKNFFKLAQGEYIAPEKIESVYLSSCPYLTQISVHGDSLQTFLVAVIGLELDITAPIIHKKIPELRGFSGKDLVDEINKSRAHRKALIVLINSFIEGLQGFEKIHNLYVGIEPLKVTDDTITPTLKVKRANAAKHFRKILENLYEEGSLIKVEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJA3</accession>
    <name>C5DJA3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0F14806p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0F14806g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554829.1">
        <property type="nucleotide sequence ID" value="XM_002554783.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJA3"/>
    <dbReference type="FunCoup" id="C5DJA3">
        <property type="interactions" value="39"/>
    </dbReference>
    <dbReference type="GeneID" id="8293058"/>
    <dbReference type="KEGG" id="lth:KLTH0F14806g"/>
    <dbReference type="eggNOG" id="ENOG502QZYP">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059029_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJA3"/>
    <dbReference type="OMA" id="YRQCVQE"/>
    <dbReference type="OrthoDB" id="5549748at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018859">
        <property type="entry name" value="BAR_dom-cont"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10455">
        <property type="entry name" value="BAR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="255"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="272"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="358" mass="40714" checksum="F396280D5AB2F8F7" modified="2009-07-28" version="1">MSNYFGGFSLNKLTNSITNVAHKTQDTLSTAIANIQLDDPQAKLSLKARKHYLQETLGTIEDISKLPPQYQFLEKKCDSLEKVCRRMLVVTKTYEVEGYDYPPNLSESLSDWWSSNKEGLFGFVSSSKKEKKLEPAEDSKEALMPRSFAQAISKAAKDSGEVMTALKTEEQKASAEEDEEDEDITSLIKMFEAWSACQHNMDQSKAEMDALMVKEFNHKLTAFVEEKFKNARTLRKKVEDSRLKFDTMRYELKMAEEQGKTEEAAQQTESQKPQEDEAEASKDSDSSDEAQKLLEKLEDEFVSNTTEAVETMGEITDSAEIINLVKLFHNFQLIYYKQCVQNLETSINSLNELEGDEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJC9</accession>
    <name>C5DJC9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">KLTH0F15422p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0F15422g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554855.1">
        <property type="nucleotide sequence ID" value="XM_002554809.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJC9"/>
    <dbReference type="STRING" id="559295.C5DJC9"/>
    <dbReference type="GeneID" id="8293086"/>
    <dbReference type="KEGG" id="lth:KLTH0F15422g"/>
    <dbReference type="eggNOG" id="KOG0769">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015166_6_3_1"/>
    <dbReference type="InParanoid" id="C5DJC9"/>
    <dbReference type="OMA" id="QFMIIES"/>
    <dbReference type="OrthoDB" id="2019556at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015217">
        <property type="term" value="F:ADP transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080122">
        <property type="term" value="F:AMP transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005347">
        <property type="term" value="F:ATP transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015228">
        <property type="term" value="F:coenzyme A transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015230">
        <property type="term" value="F:FAD transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044610">
        <property type="term" value="F:FMN transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051724">
        <property type="term" value="F:NAD transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052217">
        <property type="entry name" value="Mito/Peroxisomal_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45939:SF5">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PMP34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45939">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PMP34-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="37"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="92"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="187"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="229"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="296"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="35"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="133"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="225"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR24418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="341" mass="37282" checksum="A6B62B59813DD33B" modified="2009-07-28" version="1">MKEKSYMMIVVISQASKPQFHEPSPMKSEIDPSKADAFTHALAGGAGGALSMALTYPLVAITTRLQTQTKSSETDKLTVAETIREIYEKNGILGFFAGLESAVLGMTLSNFVYYYCYEASSRCLMRARRTQRLSTAESMLVGSIAGSVNAVIANPLWVANTRMTVDKSDRGVLATIANISKTEGLSALFSGLKPALVLVINPIIQYTVYEQLKNRVLESRQKRVLSPSWAFILGALGKLAATSSTYPYVTMKARMHLSKNEGSSPAENSKSLLSLMGEIIKRDGILGLYGGIGVKLIQSILTAAFLFFFKEGLVIWSIKLLRLLRAFSRKRKLLTTARAQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJG4</accession>
    <name>C5DJG4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">26S proteasome regulatory subunit 6A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0F16214g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554890.1">
        <property type="nucleotide sequence ID" value="XM_002554844.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJG4"/>
    <dbReference type="FunCoup" id="C5DJG4">
        <property type="interactions" value="1060"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJG4"/>
    <dbReference type="GeneID" id="8293122"/>
    <dbReference type="KEGG" id="lth:KLTH0F16214g"/>
    <dbReference type="eggNOG" id="KOG0652">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_2_4_1"/>
    <dbReference type="InParanoid" id="C5DJG4"/>
    <dbReference type="OMA" id="NKISHEH"/>
    <dbReference type="OrthoDB" id="9443236at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010604">
        <property type="term" value="P:positive regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070682">
        <property type="term" value="P:proteasome regulatory particle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080090">
        <property type="term" value="P:regulation of primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000009">
        <property type="entry name" value="26S protease regulatory subunit 6A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000076">
        <property type="entry name" value="26S protease regulatory subunit 6A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000037">
        <property type="entry name" value="26S protease regulatory subunit 6A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032501">
        <property type="entry name" value="Prot_ATP_ID_OB_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16450">
        <property type="entry name" value="Prot_ATP_ID_OB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0647">Proteasome</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="12">
        <location>
            <begin position="214"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00069320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR24453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="434" mass="48242" checksum="5F7546BB9208EDCC" modified="2009-07-28" version="1">MATLEELDAQQLPGDDEIDQEILNLPTSELQTRTKLLDNEIRIFRSELQRLSHENNVMLEKIKDNKEKIKNNRQLPYLVSNVVEIMDMDELDDEQHRESTTQGGNVNLDNIASGKAAVVKTSSRQTVFLPMVGLVDPDKLKPNDLVGVNKDSYLVLDTLPSEFDSRVKAMEVDEKPTETYSDVGGLDKQIEELVEAIVLPLKQAEKFTNMGIKAPKGALMYGPPGTGKTLLARACAAQTNATFLKLAAPQLVQMFIGEGSKLVRDAFALAKEKAPTIIFIDELDAIGTKRFDSEKSGDREVQRTMLELLNQLDGFGSDDRVKVLAATNRVDVLDPALLRSGRLDRKIEFPLPSEDSRAQILQIHSRKMTTDDSINWQELARSTDEFNGAQLKAVSVEAGMIALRNGQSQVKHEDFVEAIGEVQARKSKSVSFYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJM1</accession>
    <name>C5DJM1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">60S ribosomal protein L3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0F17556g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uL3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554947.1">
        <property type="nucleotide sequence ID" value="XM_002554901.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJM1"/>
    <dbReference type="FunCoup" id="C5DJM1">
        <property type="interactions" value="919"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJM1"/>
    <dbReference type="GeneID" id="8293182"/>
    <dbReference type="KEGG" id="lth:KLTH0F17556g"/>
    <dbReference type="eggNOG" id="KOG0746">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033361_2_1_1"/>
    <dbReference type="InParanoid" id="C5DJM1"/>
    <dbReference type="OMA" id="QRTEYNK"/>
    <dbReference type="OrthoDB" id="1611972at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000079">
        <property type="entry name" value="60S ribosomal protein L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1430.10:FF:000001">
        <property type="entry name" value="60S ribosomal protein L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.960.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000351">
        <property type="entry name" value="Ribosomal protein L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.960.10">
        <property type="entry name" value="Ribosomal protein L3, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045077">
        <property type="entry name" value="L3_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044892">
        <property type="entry name" value="Ribosomal_L3_dom_3_arc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000597">
        <property type="entry name" value="Ribosomal_uL3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019926">
        <property type="entry name" value="Ribosomal_uL3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11363">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11363:SF5">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00297">
        <property type="entry name" value="Ribosomal_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00474">
        <property type="entry name" value="RIBOSOMAL_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR24510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="388" mass="43687" checksum="337BE17A8F108575" modified="2009-07-28" version="1">MSHRKYEAPRHGHLGFLPRKRAASIRGKVRSFPKDDKSKSVALTSFLGYKAGMSTIVRDLDRPGSKFHKREIVEAVTVVDTPPMVVVGVVGYVETPRGLRSLTTVWAEHLSDEIKRRFYKNWYKSKKKAFTKYSAKYAQDGAAIERELARISKYATVVRVLAHTQVRKTPLAQKKAHLAEIQINGGSTADKVAWAREHFEKTVSVDSVFEQNEMIDVCAVTKGHGFEGVTHRWGTKKLPRKTHRGLRKVACIGAWHPAHVMWSVARAGQRGFHTRTSINHKVYRVGKGGDEGNASTEFDRTKKSITPMGGFVQYGPVNNDFVMVKGCIPGNRKRVVTLRKSLYTNTSRKALEQVNLKWIDTASKFGKGRFQTPAEKHAFMGTLKKDLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJR9</accession>
    <name>C5DJR9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">GTP-binding protein RHO1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0F18612g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554995.1">
        <property type="nucleotide sequence ID" value="XM_002554949.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJR9"/>
    <dbReference type="STRING" id="559295.C5DJR9"/>
    <dbReference type="GeneID" id="8293230"/>
    <dbReference type="KEGG" id="lth:KLTH0F18612g"/>
    <dbReference type="eggNOG" id="KOG0393">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041217_21_2_1"/>
    <dbReference type="InParanoid" id="C5DJR9"/>
    <dbReference type="OMA" id="KNGFIMF"/>
    <dbReference type="OrthoDB" id="8830751at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006075">
        <property type="term" value="P:(1-&gt;3)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01870">
        <property type="entry name" value="RhoA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000329">
        <property type="entry name" value="GTP-binding protein rhoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0636">Prenylation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="189"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="191"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00067971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR24558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="209" mass="22977" checksum="20172AEEE28CFCBC" modified="2009-07-28" version="1">MSQAGMNNASIRRKLVIVGDGACGKTCLLIVFAKGKFPQVYVPTVFDNYVADVEVDGRRVELALWDTAGQEDYDRLRPLSYPDSNVVLICFSIDLPDSLENVMEKWISEVLHFCQGVPIILVGCKVDLRNDSQVIENLRANGQEPVSQGAAQEVAEQIGAVEYIECSAKTGYGVREVFEAATRASLMGKQNKTKKTKSSGKKKKKCVVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJS5</accession>
    <name>C5DJS5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0F18766p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0F18766g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555001.1">
        <property type="nucleotide sequence ID" value="XM_002554955.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJS5"/>
    <dbReference type="FunCoup" id="C5DJS5">
        <property type="interactions" value="357"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJS5"/>
    <dbReference type="GeneID" id="8293237"/>
    <dbReference type="KEGG" id="lth:KLTH0F18766g"/>
    <dbReference type="eggNOG" id="KOG4205">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012062_0_2_1"/>
    <dbReference type="InParanoid" id="C5DJS5"/>
    <dbReference type="OMA" id="RVCQNKF"/>
    <dbReference type="OrthoDB" id="1875751at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12577">
        <property type="entry name" value="RRM1_Hrp1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12330">
        <property type="entry name" value="RRM2_Hrp1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000025">
        <property type="entry name" value="RNA-binding protein Musashi homolog 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034156">
        <property type="entry name" value="Hrp1_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48032:SF6">
        <property type="entry name" value="RNA-BINDING (RRM_RBD_RNP MOTIFS) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48032">
        <property type="entry name" value="RNA-BINDING PROTEIN MUSASHI HOMOLOG RBP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="208"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="292"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="97"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="181"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="448"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="19"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="37"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="54"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="105"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="118"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="181"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="461"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="479"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="515"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="574"/>
            <end position="588"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR24564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="588" mass="64159" checksum="630F9B322B23562E" modified="2009-07-28" version="1">MSFSDDEDFNDIYGDDSNATSTTAATTAAEPTKQEVAASAANAQSADTSKPQGAGAAASASTEAAQPAAVAQPAPAPAASSSLDQLAALQALSSNLSQLQQAASGNNNSSTESQTKDPSSTSDTAAVPATSTASAPPAMPGMPAMPSMPGMPSMTGMPGMPGMPNMQQWQQLQQLQQSMPQFQQGGASGPGSAAEQRTNKADLSRDICKMFIGGLNWETTEDNLKDYFSKYGQVTDLKIMRDNATGRSRGFGFLTFAEASSVDEVVKTQHILDGKVIDPKRAIPREEQDKTGKIFVGGIGPDVRPKEFEEFFSQWGTIIDAQLMLDKDTGRSRGFGFITYDTPDAVDRVCENKFIEFKGKRIEIKRAEPRHLQKQQQQQKTQAPFGSAQFNMFQNPMMGNFNPMFNPQAMAEYYNKMQEYYAQMQQQTGVDYSQMYQQQMQQMQQMMTGMMPGMPGMPPAASQSDTPTQEASDVPTIGSEDKAEERNDGDNNIERRVSSPPTNAPRGPRSGNPRDNGREGRDNRDGRDGRDNRNSRDGRDGRDGRDDRDNGGRGSYRSGFRNRDRSGYRGGRSGYSRRNNNGYHPYSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJV8</accession>
    <name>C5DJV8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0F19492p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0F19492g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555034.1">
        <property type="nucleotide sequence ID" value="XM_002554988.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJV8"/>
    <dbReference type="FunCoup" id="C5DJV8">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJV8"/>
    <dbReference type="GeneID" id="8293270"/>
    <dbReference type="KEGG" id="lth:KLTH0F19492g"/>
    <dbReference type="eggNOG" id="ENOG502QUA0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020178_0_1_1"/>
    <dbReference type="InParanoid" id="C5DJV8"/>
    <dbReference type="OMA" id="PLENMAM"/>
    <dbReference type="OrthoDB" id="2140105at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022703">
        <property type="entry name" value="DUF3533"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053001">
        <property type="entry name" value="MNNG_permease-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34814">
        <property type="entry name" value="NITROSOGUANIDINE RESISTANCE PROTEIN SNG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34814:SF1">
        <property type="entry name" value="NITROSOGUANIDINE RESISTANCE PROTEIN SNG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12051">
        <property type="entry name" value="DUF3533"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="298"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="340"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="376"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="401"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="435"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="467"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3533" evidence="3">
        <location>
            <begin position="97"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR24597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="506" mass="57092" checksum="01F5A318615342DA" modified="2009-07-28" version="1">MSYDRSEQLSEELSRVETGASELQHQEFTRAVVGENSDEGSSAERAAAKEEDAENEPEMPAVRLRRTESKLSRVVTGFFSDRMHTERRTLLGKFLLNHLLLGMLIMAVFSLYWGAMYKRSSHLHKVKVLAVIQDDGGDGGSAGGVATAIPQLLESVPGTWHVYNTSSFQQRYGVAGGEIDAKIHKLVHDEKYWMSLNVKPNATNALLDSVRRASAPAFNSSQYFEAFYESGRDPTNMKSTILPLMLQLEALHQNYHASVYLPAVLQNASQTMAQAARNVAQAGRMVYGQVDSRPFSDYVLLGPLQVGLIYCILLTFFQLALFGPIHGQMGQKLKPSHMLLYRYTIAFVNYFFLSLFFCLVSLAFQVDYTKTFGRGGFMVAWMTSWLLMAAVGGANENMITMVMSVAPQFLGFWMIFWVVLNISPSFYPMDLTNNFYRYGYMTPIYNGVMMFRVIFLDLYRGNLGRNYGILCAWVVVNMALFPFVMKFFIHQKKKEVMRAAAARNAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJX8</accession>
    <name>C5DJX8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0F19954p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0F19954g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555054.1">
        <property type="nucleotide sequence ID" value="XM_002555008.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJX8"/>
    <dbReference type="STRING" id="559295.C5DJX8"/>
    <dbReference type="GeneID" id="8293291"/>
    <dbReference type="KEGG" id="lth:KLTH0F19954g"/>
    <dbReference type="eggNOG" id="ENOG502RZTT">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065416_1_0_1"/>
    <dbReference type="InParanoid" id="C5DJX8"/>
    <dbReference type="OMA" id="HERACRR"/>
    <dbReference type="OrthoDB" id="2013972at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="1">
        <location>
            <begin position="46"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="278" mass="30979" checksum="80605242946C6567" modified="2009-07-28" version="1">MMSDAWGLQAKEYAEKLQKGPPKASVDAILQAVDSLLPFEKAKSILDNGCGTGLGTQTLIDKYGHRLPDLIHLVAADLSPGMIESVRMTKAAHKGNRFWDRLELQVCSVDNMSKTQNSCFSHIIASLVFFFAKPEATFSEAYRILQPGGVIGFTSFRENQWMDLRNVVKEINPGLEPAPLPAEWASEEWIRGQLEKANFIDIKIEPMEVYVELDDSLPLATLLVHSGIPEMSAVFQSLTDEEKDRAVQLIVEELAERYPKRPAKIPGILLVSSARKQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>C5DK30</accession>
    <name>C5DK30_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0F01342p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0F01342g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554268.1">
        <property type="nucleotide sequence ID" value="XM_002554222.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DK30"/>
    <dbReference type="STRING" id="559295.C5DK30"/>
    <dbReference type="GeneID" id="8292459"/>
    <dbReference type="KEGG" id="lth:KLTH0F01342g"/>
    <dbReference type="eggNOG" id="ENOG502S4MN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114639_2_0_1"/>
    <dbReference type="InParanoid" id="C5DK30"/>
    <dbReference type="OMA" id="GQFRNYY"/>
    <dbReference type="OrthoDB" id="2017405at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021475">
        <property type="entry name" value="Pants/Emi1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28052">
        <property type="entry name" value="UPF0545 PROTEIN C22ORF39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28052:SF1">
        <property type="entry name" value="UPF0545 PROTEIN C22ORF39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11326">
        <property type="entry name" value="PANTS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR23831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="85" mass="10070" checksum="136B6F5F2F6C3999" modified="2009-07-28" version="1">MSTKYPSTMSCTEAFDQLSACYSVGGQFRNYYRYGEFNACTRQLEKFKFCVLHGTDPVKIQQWYRDQAKFNAMHRGSSDEIWEER</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKA6</accession>
    <name>C5DKA6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA repair protein RAD50</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ordered locus">KLTH0F03058g</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SMC family. RAD50 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554344.1">
        <property type="nucleotide sequence ID" value="XM_002554298.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DKA6">
        <property type="interactions" value="1096"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKA6"/>
    <dbReference type="GeneID" id="8292536"/>
    <dbReference type="KEGG" id="lth:KLTH0F03058g"/>
    <dbReference type="eggNOG" id="KOG0962">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006184_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKA6"/>
    <dbReference type="OMA" id="FSDYYYR"/>
    <dbReference type="OrthoDB" id="18797at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000794">
        <property type="term" value="C:condensed nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030870">
        <property type="term" value="C:Mre11 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003691">
        <property type="term" value="F:double-stranded telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051880">
        <property type="term" value="F:G-quadruplex DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043047">
        <property type="term" value="F:single-stranded telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070192">
        <property type="term" value="P:chromosome organization involved in meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007127">
        <property type="term" value="P:meiosis I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000725">
        <property type="term" value="P:recombinational repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000722">
        <property type="term" value="P:telomere maintenance via recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007004">
        <property type="term" value="P:telomere maintenance via telomerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000593">
        <property type="entry name" value="DNA repair protein RAD50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001195">
        <property type="entry name" value="DNA repair protein rad50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038729">
        <property type="entry name" value="Rad50/SbcC_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004584">
        <property type="entry name" value="Rad50_eukaryotes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013134">
        <property type="entry name" value="Zn_hook_RAD50"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00606">
        <property type="entry name" value="rad50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18867:SF12">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18867">
        <property type="entry name" value="RAD50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13476">
        <property type="entry name" value="AAA_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04423">
        <property type="entry name" value="Rad50_zn_hook"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13558">
        <property type="entry name" value="SbcC_Walker_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51131">
        <property type="entry name" value="ZN_HOOK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="14 20" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="17" id="KW-0469">Meiosis</keyword>
    <keyword evidence="7 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 19" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zinc-hook" evidence="21">
        <location>
            <begin position="626"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="20">
        <location>
            <begin position="218"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="20">
        <location>
            <begin position="408"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="20">
        <location>
            <begin position="785"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="20">
        <location>
            <begin position="966"/>
            <end position="1091"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="673"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="676"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="CAR23907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1298" mass="149768" checksum="C8D2A2AA82979902" modified="2009-07-28" version="1">MSAIYKLSIQGIRSFDSNERETIEFGKPLTLIVGTNGSGKTTIIECLKYATTGDLPPNSKGGAFIHDPKITGEKDVRAQVKLAFMSPNDVNMIVTRNIQLLVKKTTNTFKTLEGQLVAINRGERTTLSSRAAELDTQVPLYMGVPKAILDYVVFCHQEDSLWPLSEPSNLKKRFDEIFQAMKYTKALDNLKAIKKDMSVESKLLKQSVEHLKVDRDRSRATKINISTLEKRIDEYKEQARQAEEQLDEITEQSDKLFKSNQEFQEVLSKMDGLKHSKNSLEEQINRLEGSTEVLNLPKVELERLVANFSSTLEEKQGQIKELEDILVTKRHVLEQTREQYNTLISQQGELNARKSTHEDHIKELSTLKAELREKDGLSGADILTDLESKVLSSNSHFDLITAQNKQCIERLRNQISELQSTRAKQEQHLIYCGNDKNKLASEIDSLESKLKAMSNLEEDLAKEEDKMNNFKTRLDTHESEDEINSIGFQIKEKNSSILLMEDELEKNQRELLKVNEQADLSAKYSLIQQSLDKKRVEVDDLVSKVTADKRAQEWNLWSQSDSEFEFRKAHISLQKEYALLTQENNKITKGLAEKEFLLAQLRREKDANDEKLVMLDKKITEGLPEDCSIEDYITIMEETEESYRVAVENLKMHKTTLEFNLKALEVAKENNCCYLCQREFHDGKEQSTLLKELQSRTDTKFEETLTKIVEEEKDYLDSLRRLESDVSSLKVLKKSQEALTLDYEKVSKDLQQKTSEAAESDAKVQMVKKDIDHAELVLRPLVENLSRVTRELKALGSEFQNISDEVRLRNGLDSNFKTVEELQAAQKQLNDSLRDARKEIGLLQEAKEMKSREYNNLLGLIREKSFKVDELKKQLRDRENITSTIKDRKSAISQTDTIIQTVTSTLTDLSVSINEAKNKLSLVSDKANTEEVRVKGELDSLRSKRDRYAMLWSHIRDFEDKYSPQLENCKNDIELSRKMIHSLKDEVETLDNRMREQSQKVKDSANEQKNLRLNIDLINLKLKLVKVEKDLRDLHVQNAEAQRDKYQQESTRLRALFEKLSSDNAGRMGEIKQLQNQIRSLLNQLNTDYKDVDSIYQKEWVQLQTKTLVTDDIDVYSKALDSAIMKYHSLKMHDINRIIDELWKRTYSGTDVDTIKIKSDEVTTSARGKSYNYRVVMYKQDAELDMRGRCSAGQKVLASIIIRLALSETFGVNCGVIALDEPTTNLDEENIESLAKSLNNIIEFRRHQKNFQLIVITHDEKFLRYMGAADFTDHFYKVKRDDRQKSQIEWVNINKVTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKD0</accession>
    <name>C5DKD0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0F03652p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0F03652g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554368.1">
        <property type="nucleotide sequence ID" value="XM_002554322.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKD0"/>
    <dbReference type="FunCoup" id="C5DKD0">
        <property type="interactions" value="143"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKD0"/>
    <dbReference type="GeneID" id="8292562"/>
    <dbReference type="KEGG" id="lth:KLTH0F03652g"/>
    <dbReference type="eggNOG" id="KOG1281">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005170_8_0_1"/>
    <dbReference type="InParanoid" id="C5DKD0"/>
    <dbReference type="OMA" id="DINNFPW"/>
    <dbReference type="OrthoDB" id="10260443at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006797">
        <property type="term" value="P:polyphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01115">
        <property type="entry name" value="SLC13_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14478">
        <property type="entry name" value="SPX_PHO87_PHO90_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004680">
        <property type="entry name" value="Cit_transptr-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10283:SF110">
        <property type="entry name" value="INORGANIC PHOSPHATE TRANSPORTER PHO87-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10283">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 13 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03600">
        <property type="entry name" value="CitMHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03105">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="435"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="486"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="524"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="566"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="609"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="649"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="693"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="719"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="745"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="783"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="828"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="880"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="domain" description="SPX" evidence="8">
        <location>
            <begin position="1"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="176"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="179"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="230"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR23931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="903" mass="99950" checksum="215E0207337D0BB9" modified="2009-07-28" version="1">MKFSHSLKYNAVPDWQEHYLNYSQLKKLIYSLQAQDLQVVDEGGRVDTERLNALEGSSKALKKLKGKFQLGRSGKNGKGRKAGEGDADAEGDVQVETFELSDIKKKSAQNKLQALVDFDRRSSLSSDRTLFNPQDTFLSKLIDERSKIDDFYKKLEAQLYGRFATVVSDLQKSGALVDTHSRPHGGDGDGGDHESFELRTQPEHQLRRRESLASKRTTNDSLAPGRASQPEDDEEEDEEEEFDEHTHENTALLNYSDFNVKSQKKAIIKKNLIDLYVDLVQLKSFIELNRIGFFKITKKFDKTLECNVRNELIESGEFFKDTYVFQPLTLEVLDSKITKVIEFYAFITNADLALCKEELRSYLRDFIVWERNTVWKDMLGLESQNNTIASAGRPNAKGDIGALESNTHLEYRTWNLPKPIKCRYFTWSCIKVPQLFFTLKALKLYIIIACTIILLAVPTFNDEAQHRCMALVACVAFLWASEALPLFVTALLVPLLVVLFRVMKSDGKVMNAADASSTILAKMWSSTIMVLLAGFTLAAALSKYNIARVLASYLLTFAGTKPRNVLLMIMGVCFFLSMWISNVAAPVLTYSLIQPVLKSLGYESPFAKAIVLGVALSANVGGMASPIASPQNIIAMDYLKDYNVGWGQFFAIALPSSFLAMLTIWLLLLCTFKINGTQLKKYTPIKEKFTLKQYYIMVVTLATILLWCVLSKLENVFGSSGQIAVIPIVLFFGTGLLGTQDINNFPWAIVILAMGGIALGGAVSSSGLLTTIAGALQRRVMDFPLYAVMAIFGIVMLVVGTFVSHTVSAIIIIPLMQEVGDKLPGAKSAPILVFACTLLSSCGMGLASSGFPNVTAISMTDEVGNRYLTVNNFITRGVPASFLAFICVITLGFGIMNSVLKGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKF4</accession>
    <name>C5DKF4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0F04246p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0F04246g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATP12 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554392.1">
        <property type="nucleotide sequence ID" value="XM_002554346.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKF4"/>
    <dbReference type="FunCoup" id="C5DKF4">
        <property type="interactions" value="716"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKF4"/>
    <dbReference type="GeneID" id="8292587"/>
    <dbReference type="KEGG" id="lth:KLTH0F04246g"/>
    <dbReference type="eggNOG" id="KOG3015">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047893_1_2_1"/>
    <dbReference type="InParanoid" id="C5DKF4"/>
    <dbReference type="OMA" id="QGWVMGL"/>
    <dbReference type="OrthoDB" id="5322896at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033615">
        <property type="term" value="P:mitochondrial proton-transporting ATP synthase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3580.10">
        <property type="entry name" value="ATP12 ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2180.10">
        <property type="entry name" value="ATP12-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011419">
        <property type="entry name" value="ATP12_ATP_synth-F1-assembly"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042272">
        <property type="entry name" value="ATP12_ATP_synth-F1-assembly_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023335">
        <property type="entry name" value="ATP12_ortho_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21013:SF10">
        <property type="entry name" value="ATP SYNTHASE MITOCHONDRIAL F1 COMPLEX ASSEMBLY FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21013">
        <property type="entry name" value="ATP SYNTHASE MITOCHONDRIAL F1 COMPLEX ASSEMBLY FACTOR 2/ATP12 PROTEIN, MITOCHONDRIAL PRECURSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07542">
        <property type="entry name" value="ATP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160909">
        <property type="entry name" value="ATP12-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR23955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="317" mass="35504" checksum="2C25A4D31D3BDD2F" modified="2009-07-28" version="1">MLRHATFRSLRSAHLCSVRAYARATSLGIDSSVENNLQTETNRASKTLQKFWNDVSVKECDTHLTVMLDNKPLRTPLGNALSVSKDRKLLALLLQNEWSSMSSLAIKPHSLPITSIVSRCIDLEYTAKPGSDPELVAKIGGDRSVISESLLRYLDTDTLMCLSPRAEYEGALRAAQDELYLPVISQVEKFLSRFAQDPVKLQILDADVHGLKGNLQDKNTKEAALAYLNSLSMWDFAVFEKAVLTTKSFLCGLLLLHNKSQNPSSDMKVTMEKIAQFATLETIYQVERWGEVEDTHDVDKRDVRRNINAASIVAFKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKY8</accession>
    <name>C5DKY8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">T-complex protein 1 subunit zeta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">CCT-zeta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0F08580g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554576.1">
        <property type="nucleotide sequence ID" value="XM_002554530.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKY8"/>
    <dbReference type="FunCoup" id="C5DKY8">
        <property type="interactions" value="1188"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKY8"/>
    <dbReference type="GeneID" id="8292782"/>
    <dbReference type="KEGG" id="lth:KLTH0F08580g"/>
    <dbReference type="eggNOG" id="KOG0359">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008891_3_1_1"/>
    <dbReference type="InParanoid" id="C5DKY8"/>
    <dbReference type="OMA" id="LHPRIMT"/>
    <dbReference type="OrthoDB" id="10052040at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03342">
        <property type="entry name" value="TCP1_zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000058">
        <property type="entry name" value="T-complex protein 1 subunit zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000004">
        <property type="entry name" value="T-complex protein 1 subunit zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012722">
        <property type="entry name" value="Chap_CCT_zeta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02347">
        <property type="entry name" value="chap_CCT_zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR24139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="544" mass="59339" checksum="C790E951C6C3A91B" modified="2009-07-28" version="1">MSLQLLNPKAESLRRDAALKVNVTSAEGLQSVLETNLGPKGTLKMLVDSAGNIKLTKDGKVLLTEMQIQSPTAVMIARAAAAQDEITGDGTTTVVCLVGELLKQAHRLVQEGVHPRMITDGFEIARRETMEFLNSYKIDKPAEEDMDREFLLQVARSSLSTKVAPELAEVLTPIVTDAVLSVKNADSRSLDLHMVEIMQMQHLSAKDTSFIRGLVLDHGGRHPDMPTRVENAHILILNVSLEYEKTEVNSGFFYSSADQRDKLAASERKFVDEKVKKIIDLKNEVCGLDSKRGFVIINQKGIDPMSLDILAKHGILALRRAKRRNMERLQLVTGGEAQNSVDDLSPSVLGFSGLVYQETIGEEKFTYVTENRDPKSCTILIKGATYHALAQTKDAVRDGLRAVANVLKDKSVVPGAGAFYLAASNHLRNINGNKLGAKGKKKAGIQAFAEALLVVPKTLIKNSGYDALDVLATCQDELEEEEGRIVGVDLNVGDSCDPTIEGIWDSYRVIRNAITGSTGIASNLLLCDELLRAGRSTLKEGPPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DL13</accession>
    <name>C5DL13_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0F09130p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0F09130g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554601.1">
        <property type="nucleotide sequence ID" value="XM_002554555.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DL13"/>
    <dbReference type="FunCoup" id="C5DL13">
        <property type="interactions" value="803"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DL13"/>
    <dbReference type="GeneID" id="8292807"/>
    <dbReference type="KEGG" id="lth:KLTH0F09130g"/>
    <dbReference type="eggNOG" id="KOG0710">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057192_0_0_1"/>
    <dbReference type="InParanoid" id="C5DL13"/>
    <dbReference type="OMA" id="PANYEAE"/>
    <dbReference type="OrthoDB" id="5511210at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="CDD" id="cd06464">
        <property type="entry name" value="ACD_sHsps-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031107">
        <property type="entry name" value="Small_HSP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11527">
        <property type="entry name" value="HEAT-SHOCK PROTEIN 20 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="5">
        <location>
            <begin position="194"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="104"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="148"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="26"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="110"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="148"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR24164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="332" mass="37290" checksum="AE30DC93DD0CCF04" modified="2009-07-28" version="1">MSFTQPTVSIYDILEALSSQQDRSNARRRPHHSNYVRPRTRGAYGNPYVQSPYYYAERPQTTYYRPRYYDENEYESPYVVGGRGGYGPQPDVLPAILRAFMAQQSSEHNEPEEAELPDQLEQDQEAAEVPQIAAQPLAQALADEVLEQAEAESAEENGAEVAGEATDAEQKQAPRSGASSPIPEPLQVSKPQVRLDLPFSPEVNVYDTPEAYVVVLALPGANSKSFKIDYHPSSHELLIKGSLDRKIDVNDKFLRISELKGGKFERSVKFPVVPRIKDEEIKATYANGLLQIKTPKILDESSQHQPRRRIVIEDVPDEELVFEENPNPPTSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLA1</accession>
    <name>C5DLA1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0F11286p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0F11286g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554689.1">
        <property type="nucleotide sequence ID" value="XM_002554643.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLA1"/>
    <dbReference type="FunCoup" id="C5DLA1">
        <property type="interactions" value="162"/>
    </dbReference>
    <dbReference type="GeneID" id="8292902"/>
    <dbReference type="KEGG" id="lth:KLTH0F11286g"/>
    <dbReference type="eggNOG" id="KOG1286">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007946_12_1_1"/>
    <dbReference type="InParanoid" id="C5DLA1"/>
    <dbReference type="OMA" id="NTIIHYW"/>
    <dbReference type="OrthoDB" id="10062876at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF15">
        <property type="entry name" value="GENERAL AMINO ACID PERMEASE AGP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="73"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="101"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="181"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="215"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="265"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="307"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="364"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="409"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="438"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="484"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="516"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="6">
        <location>
            <begin position="72"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR24252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="582" mass="63932" checksum="C1C9B0DFA0672391" modified="2009-07-28" version="1">MLLPTEASGTRETSLGAQKGKTEFKYQTVKVASHQDSVVSIDAKQTITGYLTAEAESDMEDSVTRRTLRERHVLLIGISGVIGTALFVSIGKGLFRGGSVFLLLGMALWCIPMLCITVSTAEMVCYLPLDSPFLCLASRCVDDSLAIMAGWNFWFLECVQIPFEIVAVNTILHYWRDDYSAAIPLVVQVILYFAISVLAVRFYGEMEFWLASFKIILAAGLFVFTLVAMLGGNPKKDRFGFRNFADAPFKQYFPSGKPGSPSAGYFQGFLACLIQASFTIAGPDYVAMIAGETRLPRKILPVAFKQVFNRLTILFLGSSLCVGILCSANDPSLMAAISEARPGAGASPYVLAMKNLGIKTLPDVVNAALVTAAFSAGNAYTFCSSRTLHGMALVGNAPAVFKRCNRHGVPIYAVAVSLTWALLSLLQLNKNSAVVLNWLINMITASQLINFAVLCVTYLYFRRAYHAQCGALPELPFKSWGQPYTALVGLVAVLIMVLVQGYAVFCSSLWDVQEFLFCYLMIFIDIAIYVVHKFVVQRGRCWPRAPKAVDLVTGLLAVERHEREAGFDRYVFFTERASPLRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLI2</accession>
    <name>C5DLI2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0G00924p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0G00924g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the MSS1-MTO1 complex that catalyzes the 5-carboxymethylaminomethyluridine (cmnm(5)U) modification at the 34th wobble position (U34) of mitochondrial tRNAs.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MnmG family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555080.1">
        <property type="nucleotide sequence ID" value="XM_002555034.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLI2"/>
    <dbReference type="FunCoup" id="C5DLI2">
        <property type="interactions" value="806"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLI2"/>
    <dbReference type="GeneID" id="8293338"/>
    <dbReference type="KEGG" id="lth:KLTH0G00924g"/>
    <dbReference type="eggNOG" id="KOG2311">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007831_2_2_1"/>
    <dbReference type="InParanoid" id="C5DLI2"/>
    <dbReference type="OMA" id="CNPAMGG"/>
    <dbReference type="OrthoDB" id="3329at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070899">
        <property type="term" value="P:mitochondrial tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000145">
        <property type="entry name" value="tRNA uridine 5-carboxymethylaminomethyl modification enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.570:FF:000001">
        <property type="entry name" value="tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000002">
        <property type="entry name" value="tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.570">
        <property type="entry name" value="GidA associated domain, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00129">
        <property type="entry name" value="MnmG_GidA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049312">
        <property type="entry name" value="GIDA_C_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004416">
        <property type="entry name" value="MnmG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002218">
        <property type="entry name" value="MnmG-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020595">
        <property type="entry name" value="MnmG-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026904">
        <property type="entry name" value="MnmG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047001">
        <property type="entry name" value="MnmG_C_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044920">
        <property type="entry name" value="MnmG_C_subdom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040131">
        <property type="entry name" value="MnmG_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00136">
        <property type="entry name" value="mnmG_gidA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11806">
        <property type="entry name" value="GLUCOSE INHIBITED DIVISION PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11806:SF0">
        <property type="entry name" value="PROTEIN MTO1 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01134">
        <property type="entry name" value="GIDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21680">
        <property type="entry name" value="GIDA_C_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13932">
        <property type="entry name" value="SAM_GIDA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01228">
        <property type="entry name" value="GIDA_assoc_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01280">
        <property type="entry name" value="GIDA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA uridine 5-carboxymethylaminomethyl modification enzyme C-terminal subdomain" evidence="7">
        <location>
            <begin position="591"/>
            <end position="662"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00054993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR24643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="682" mass="74605" checksum="62DFBF8BD5836093" modified="2009-07-28" version="1">MLSASLNTNKRTLGSIFKLAQRSICLNIELRSALDKLPDSGVVVVGGGHAGCEAAAGAARSGAPTVLVTPFLDKIGTCSCNPSMGGVGKGTLLREVDALDGVAPKVTDLAGIQFKMLNRSRGAAVWGPRAQIDREIYLREMQKVLKSYANLTLYQGKVQDLVIDRQVAGEGKDSHQKHSTVVGVILEDGTLLKASKVVITTGTFLSAEIHIGAKSIPAGRIGEGASYGLSKTLDEAGFKLGRLKTGTPARLDGRTINFESLEKQYGDTPPNPMSFMNEDVAIKDQVLCYGTHTTPEVHEYLRSNLHQAPHIRETIKGPRYCPSIEAKIIRFADKSSHRIWLEPEGLDSHVVYPNGISNSMPENVQLKFMRMIPGLEQVEILQPAYGVEYDYIDPRSLAKSLEAKPVKGLYLAGQINGTTGYEEACAQGIFAGINAGLAHQGKPPLEILRSDAYLGVLIDDLITKGVEEPYRMFTSRSEFRISVRADNADFRLTPLGHAAGVVSDNRYASYINQKALYSEIVTKLLNFELSAKRWNGSLGVPVADGAKNRSAWEMLRFNGIDLNTLGDKFPELGIDPSQIPKHVLLKIDVEGKYAPFIKKQNQFIKAFQADEGMLLPPNFDYSILPSLSSECKTILNLVQPSTIGQARRIQGITPASLFELYKLVRFKQNDLSAETRMECQEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLL9</accession>
    <name>C5DLL9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0G01738p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0G01738g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555117.1">
        <property type="nucleotide sequence ID" value="XM_002555071.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLL9"/>
    <dbReference type="FunCoup" id="C5DLL9">
        <property type="interactions" value="119"/>
    </dbReference>
    <dbReference type="GeneID" id="8293375"/>
    <dbReference type="KEGG" id="lth:KLTH0G01738g"/>
    <dbReference type="eggNOG" id="ENOG502RI27">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007020_0_0_1"/>
    <dbReference type="InParanoid" id="C5DLL9"/>
    <dbReference type="OMA" id="EDIWKFH"/>
    <dbReference type="OrthoDB" id="4069593at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="221"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="293"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="445"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="483"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="508"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="649"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="839"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="898"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="515"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="539"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="658"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="692"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="860"/>
            <end position="871"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="917" mass="102660" checksum="F52D6D86FC0E6451" modified="2009-07-28" version="1">MLDPGPSTPTKQQGVEHDKQELPSCLKSSEKRRSSPYNHKHSTSQVRFSLPLEDDSQSLLDEDEEPVRVSPTKVVFPKGSEEDMTTHFVDLHNKIMVDVPEEIWRFHNERNAQQLQKMSVKGQKGHGRAQSLQSIIAGTIQSYQSPAAENTHSRSLSCTSSRLKPSELYLRSDSPLNKYKVPVPLEVTLPPYLSPDKKTKRRESMIYDGEGYSLFFEDSDVQEERDPFDPNSSADRSESEGSDISIPSASHDYSFEVGQDIDKILGIDEDANVNLKNQVRNLLNKSPSKKAFLSLEQPQLPKLPRSQPKVEPFQEEKPVKLKLPPSQLKTKPTQEEKPVEITKSYALKILSSPSKTITLPDFNEPVSSSNRNSLAEFFARVDCEDNTHPVTVEHEGKDEINEFFKFPAIQQEDKTTETQPIDENLQVNEGELDNSFERRRKMLQNRASTDDFRTRHSHARSRSFHNSQEMFNAKLTSSGILSSNLEAPERSPKRSSIKSVTFKEGLPGADVEANAKSTPTKSSTLQANRNCELDKGPVSEEEEEEEEESFSFTLSEHEQKDSAESGIIARPAELLETAHKNSQPAQDTDELSHKSGLSYHNSSSSLAMDFFSDVISDADSSDFIHSTGTSSVKNLSSSKSDSSISLIGANAKPGDNITPLSTYNSFKTPLDSPQTANDPSLIECRLTHDNRLSPSRQLSNDRSQSSECSADTMPSQPSSATTYSHNDWKQSMEVFSYINKKSGNNFVSGRRAKRGNNPALVAGEIQPSYSGYRVTKEEIGGRLVDIILLDEPGNNDCAPKRRPRSYHEDALQHYDQVLALCDMTADRAKSVIMNLVEDPSHTNQVSEPLSRPVPPPSYLKSHEKPIKENRSPKGLAHTSLNNNMPSRPQKYISNLEKLRRASKERSNSPDRRLRENC</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLV2</accession>
    <name>C5DLV2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0G03784p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0G03784g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555200.1">
        <property type="nucleotide sequence ID" value="XM_002555154.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLV2"/>
    <dbReference type="FunCoup" id="C5DLV2">
        <property type="interactions" value="4197"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLV2"/>
    <dbReference type="GeneID" id="8293466"/>
    <dbReference type="KEGG" id="lth:KLTH0G03784g"/>
    <dbReference type="eggNOG" id="ENOG502RPD7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032750_0_0_1"/>
    <dbReference type="InParanoid" id="C5DLV2"/>
    <dbReference type="OMA" id="LNMACGN"/>
    <dbReference type="OrthoDB" id="5380163at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14688">
        <property type="entry name" value="bZIP_YAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000067">
        <property type="entry name" value="BZIP transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.100">
        <property type="entry name" value="YAP1 redox domain. Chain B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050936">
        <property type="entry name" value="AP-1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013910">
        <property type="entry name" value="TF_PAP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023167">
        <property type="entry name" value="Yap1_redox_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40621:SF6">
        <property type="entry name" value="AP-1-LIKE TRANSCRIPTION FACTOR YAP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40621">
        <property type="entry name" value="TRANSCRIPTION FACTOR KAPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08601">
        <property type="entry name" value="PAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111430">
        <property type="entry name" value="YAP1 redox domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="7">
        <location>
            <begin position="32"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="115"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="197"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="340"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="434"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="48"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="115"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="147"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="161"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="209"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="228"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="249"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="460"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="474"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR24763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="569" mass="62748" checksum="A7FBDEAA5887577E" modified="2009-07-28" version="1">MSTVKRQLEASTPPADLPKRKGSKPGRKPLDTEAKNKRTAQNRAAQRAFRERKERKMRELEEQVERLEKVREQSEMESEFLRSQLQMLIAEIQKYRPQQSSDSQVLKYLAEAEERCKAAPVEKRTGRNAARSSISTSNRDDSENASRSRSRSTSLSEHNTLDTSRPQRYSENSSPADQDEAAKINANVQRKMNFTFEYPKDMPPANGAFPSPSGSTSTTSNHSFSHRPFSQLSNTPRTSSGSLDFLGDSASNTQSLPKFPTSESGNGDFDFNTHFDEQVSVFCTQMNEVCGTRECPLPQALPPSTAASVTPRPISPIQEEQPELSKPAQAKIPAADAFLKQAQQQQPPNAWDSPQFGHSGFGMGTEDPTNRWLFPDFSSGNMSSTVRAPSAKIDSVPFIDTSMAFPTDQPDALLPQNSDDVLNQFFEEDPIVSQLTTEESNYDPFKPDLQPSADSETSKHSVSSCSNDSDPSRAESNASTALTEPNYTIDKTSVPSAVLAAEDADVVPARDGLLLKCSEIWDRITAHPKYSDIDIDGLCMELRTKAKCSEKGVVVNSDDVQSALAKHMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>C5DM61</accession>
    <name>C5DM61_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0G06248p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0G06248g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the RNR ribonuclease family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555309.1">
        <property type="nucleotide sequence ID" value="XM_002555263.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DM61"/>
    <dbReference type="FunCoup" id="C5DM61">
        <property type="interactions" value="290"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DM61"/>
    <dbReference type="GeneID" id="8293577"/>
    <dbReference type="KEGG" id="lth:KLTH0G06248g"/>
    <dbReference type="eggNOG" id="KOG2102">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002333_0_3_1"/>
    <dbReference type="InParanoid" id="C5DM61"/>
    <dbReference type="OMA" id="DDVWGSK"/>
    <dbReference type="OrthoDB" id="372421at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.690:FF:000001">
        <property type="entry name" value="Cell wall biogenesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.700:FF:000002">
        <property type="entry name" value="Cell wall biogenesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000100">
        <property type="entry name" value="Cell wall biogenesis protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.690">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041505">
        <property type="entry name" value="Dis3_CSD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041093">
        <property type="entry name" value="Dis3l2-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001900">
        <property type="entry name" value="RNase_II/R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022966">
        <property type="entry name" value="RNase_II/R_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050180">
        <property type="entry name" value="RNR_Ribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033771">
        <property type="entry name" value="Rrp44_CSD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23355:SF9">
        <property type="entry name" value="DIS3-LIKE EXONUCLEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23355">
        <property type="entry name" value="RIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17877">
        <property type="entry name" value="Dis3l2_C_term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17849">
        <property type="entry name" value="OB_Dis3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00773">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17216">
        <property type="entry name" value="Rrp44_CSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00955">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01175">
        <property type="entry name" value="RIBONUCLEASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RNB" evidence="8">
        <location>
            <begin position="670"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="79"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="421"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="90"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="122"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="160"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="181"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="191"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="211"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="250"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="276"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="325"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="434"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="451"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="479"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR24872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1225" mass="136213" checksum="40BCA958C1322682" modified="2009-07-28" version="1">MSSSDRKNEDRFVATTGGRKNPKQIHIAHRRSASELTNLMIEQFTLQRQLEIVQAQQQQLIAQQQQLAQQSGQYLNPVGSGNASLGAPGGPIPSFVPQPPHPHYNAPSPSPSQGNRSRSHSRNSSGYYNNNEFAPPGHRKTGSQSSVYGHSRRHSLGLSEAKKAAAEEQAKRASGSGISIKIDDAEKSESVESPLSNAIEQSTYKFPANTGDAQRGTTPQTSRTSHMSPQKSFQFPSVPSNNKENREEFSNVSTPNRNANAKGKDVSGINNNWRLQQQPTPQSNQNTPSHPFHHKKSQSREAPFGGLEPPPVFQPGHRSRNSNASTHSFGSGNNGSNQNGRKSLFAPYLPQANIPELIEEGRLVCGILRVNKKNRSDAWVSTDGVLDADVYICGSKDRNRALEGDLVAVELLMVDDVWGSKKEKEEKKRRKDANNQQDILNSNDDYHNDASSRMANPTNSSSSFGSTEKEDQSSPSKGSVKRKGSLKQRPTQKKNDDVEVEGQSLLLVEEEEISDSYKPLYAGHVVAVLDRIPGQLFSGTLGLLRPSQQANNEAHKPQKPKIVWFKPTDKKVPLIAIPTEQAPKDFVENADKYSNKLFVASIKRWPITSLHPFGTLVSQLGEINEPETEIDAILRDNNFLSDEYLDPHDRSKERSTFQPTPLSEENIQSRKIFLNTTAVFETDSLSDLALHTKVLDDGSLEVGCHILDVTAHIEEGSSLDRRARKRSSGVFIPQKVVHLLPQTLNDALTLKEGKTSATISVVHRFDKSFKVLSTWIGETLITPESSITFQEINEQVSSGSLSRFVSQIQDVATALCRKRLSSPSVSLKPELCLLESLDDEKVKVDLNIFQSSAATSIVTEIERHVNSIVAETIYQKLGDLAFLRRQSKPIATKLASFEKKVTKLGYEVDITDSQSLLSSILAIEDEATRTGVEILLFKALSRAKYFVAGKVESDQYDHFALNLPLYTHFTAPLRRYADHVVHRQLKSVLNDSSYKENDDSLKITSEYCNFKKDCAHHAQEQAIHLLLCKTINDMGNSTGQILTMATVLQVYESAFDVFIPEFGIEKRVHGDQLPLIKAEYDGTNRVLELYWQAGVDSATFVPADEKNPKSYRNSIRNKFKSASNEIASIQYQKTLEGENLLTEELAGQLSNLHIDPPALKMPDPRKDTEPLERFLKTAVTRTEGDKDIQVIRELQQVPILLRAEVGMALPCLTVRTLNPFQSRKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMH8</accession>
    <name>C5DMH8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0G09064p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0G09064g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555426.1">
        <property type="nucleotide sequence ID" value="XM_002555380.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMH8"/>
    <dbReference type="FunCoup" id="C5DMH8">
        <property type="interactions" value="31"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMH8"/>
    <dbReference type="GeneID" id="8293703"/>
    <dbReference type="KEGG" id="lth:KLTH0G09064g"/>
    <dbReference type="eggNOG" id="ENOG502S2QF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108124_0_0_1"/>
    <dbReference type="InParanoid" id="C5DMH8"/>
    <dbReference type="OMA" id="WDLITAW"/>
    <dbReference type="OrthoDB" id="4067310at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097196">
        <property type="term" value="C:Shu complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000725">
        <property type="term" value="P:recombinational repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19478">
        <property type="entry name" value="Csm2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031783">
        <property type="entry name" value="Csm2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16834">
        <property type="entry name" value="CSM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="173"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="220" mass="24670" checksum="C97FD981E4C25D35" modified="2009-07-28" version="1">MEPAPALLPNDKVVLWFDSDEECISKMMATFLTVDSAEQSAKTLYFIDAMDRFPIKEFCRLVPPKENAAVYENVRIMASLDMEELSSVVSQVVSSAQMSHSTRLRDPSAPQPQVCVVIRGLDVIFRNTALKDQARAHLLLKDIMLRVRMMANTDPLLLKAVLLFEVQQKPRGINLPGSNDSSQNAPPPSKRPRSDYRSGAPNNLNSIAVYLMKFYADRVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMJ7</accession>
    <name>C5DMJ7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0G09504p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0G09504g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555445.1">
        <property type="nucleotide sequence ID" value="XM_002555399.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMJ7"/>
    <dbReference type="STRING" id="559295.C5DMJ7"/>
    <dbReference type="GeneID" id="8293723"/>
    <dbReference type="KEGG" id="lth:KLTH0G09504g"/>
    <dbReference type="eggNOG" id="KOG0254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012970_2_1_1"/>
    <dbReference type="InParanoid" id="C5DMJ7"/>
    <dbReference type="OMA" id="VAQHEYI"/>
    <dbReference type="OrthoDB" id="2241241at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015343">
        <property type="term" value="F:siderophore-iron transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000197">
        <property type="entry name" value="Siderophore iron transporter 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF92">
        <property type="entry name" value="GLUTATHIONE EXCHANGER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="123"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="147"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="180"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="209"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="272"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="302"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="340"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="381"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="407"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="435"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="473"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="545"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="582"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="11"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="603"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="614"/>
            <end position="624"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR25008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="624" mass="68785" checksum="4E1F422C396D5ACD" modified="2009-07-28" version="1">MSLKMLGFNKGPEEKEAGDAMERSRRADEALRRQKDVGVKNIEVYAAQYDHVAYRVALFFSLFLIAYAYRLDGLVRSKLEAYATDSYGNHSLLSTVNCIKTVMAAGGMVAYARASDMFGRMEMLAISIVLVVVGTIVECQSDTVSKFATGACLYQLGKAGLALILQVIAVDFSNLNWRMVALYVPALPTIINTWVSGDVISAIGSDWEWGIGMWAFIIPLSCIPLFCCLVHMRYLAHKHAPEQLGKQRQLYSMASWSEYLIEVFFWRLDSVGLILVVGALGCVLVPFTLAGGLSDKWRTAQIIVPEVIGWCVALPLFVLWEAKFARFPLTPRSVLKDRGVIAALVIAFHIHFCVYMQADYMYTVLVIAVHESVKAAQRITTLFNFVSIITGTLLGFVIVYVKRTKKFILFGVCVWFVAFGLLVHFRGDESSHSGIIGALCLLGFGGGFFNYSAQVSMQASTAAHQDLAVVTSLYLACYNIGSAFASAISGAIWTNVLPKELNKRITEKQVATLAYSSPFKFIIKYKWGTPQRVAVVHAYQKVQRILCIVPLCFCVTLLIAAFLLRDKKLSEVVALGELEHGHSESASSTGDAGASHELSSTENELHNESEKSSNTRVQNNPVTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMX6</accession>
    <name>C5DMX6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0G12452p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0G12452g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VPS37 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555574.1">
        <property type="nucleotide sequence ID" value="XM_002555528.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMX6"/>
    <dbReference type="FunCoup" id="C5DMX6">
        <property type="interactions" value="47"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMX6"/>
    <dbReference type="GeneID" id="8293856"/>
    <dbReference type="KEGG" id="lth:KLTH0G12452g"/>
    <dbReference type="HOGENOM" id="CLU_109465_0_0_1"/>
    <dbReference type="InParanoid" id="C5DMX6"/>
    <dbReference type="OMA" id="YVTKFHP"/>
    <dbReference type="OrthoDB" id="4035847at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000813">
        <property type="term" value="C:ESCRT I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072666">
        <property type="term" value="P:establishment of protein localization to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043162">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.660">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037202">
        <property type="entry name" value="ESCRT_assembly_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029012">
        <property type="entry name" value="Helix_hairpin_bin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009851">
        <property type="entry name" value="Mod_r"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07200">
        <property type="entry name" value="Mod_r"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140111">
        <property type="entry name" value="Endosomal sorting complex assembly domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51314">
        <property type="entry name" value="VPS37_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="5 6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="VPS37 C-terminal" evidence="9">
        <location>
            <begin position="99"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="57"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="13"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR25137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="178" mass="20642" checksum="D8FE0ECFFED9276B" modified="2009-07-28" version="1">MDLPHLPPKPQKIESQPSSNQASLPKNVNLLPFVVVEDLLTKHQSELEDYVLRLNDCQDIEKQNSQFQSKLKKLLQLFESTETRRRELNVELSSLQKLEAEYEIKWEQLNNLVSRSFSHEALAVTIQNNLEILESQSSVAEANFKGDVDQFLGQFLELRTKYHLQRKLLHSWNQAGDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>C5DN11</accession>
    <name>C5DN11_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0G13288g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. NIM1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555609.1">
        <property type="nucleotide sequence ID" value="XM_002555563.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DN11"/>
    <dbReference type="FunCoup" id="C5DN11">
        <property type="interactions" value="285"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DN11"/>
    <dbReference type="GeneID" id="8293892"/>
    <dbReference type="KEGG" id="lth:KLTH0G13288g"/>
    <dbReference type="eggNOG" id="KOG0583">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002664_5_1_1"/>
    <dbReference type="InParanoid" id="C5DN11"/>
    <dbReference type="OMA" id="NSADYRP"/>
    <dbReference type="OrthoDB" id="1928777at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14077">
        <property type="entry name" value="STKc_Kin1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.80">
        <property type="entry name" value="Kinase associated domain 1, KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028375">
        <property type="entry name" value="KA1/Ssp2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001772">
        <property type="entry name" value="KA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF82">
        <property type="entry name" value="KP78A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02149">
        <property type="entry name" value="KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103243">
        <property type="entry name" value="KA1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50032">
        <property type="entry name" value="KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="184"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="KA1" evidence="13">
        <location>
            <begin position="1093"/>
            <end position="1142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="112"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="150"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="645"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="812"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="695"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="819"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR25172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1142" mass="125527" checksum="6AF426C1E134C7CA" modified="2009-07-28" version="1">MAPDFSLLLASPLLAHCQPACKWTLLLLLTGFTTSAFGSYTVPSCKSGKNFGKGAIRRLQRGEHQKRTIEPLPHLASYGSAYPGFQMPSPSTNDYHVNREFRIGAASSNPAAIAPATPRMMGKQSNSSESSSVNGGLMPAVDVATTISTHTSAAQGAAHSQPVPHPTPAATPAKQFHRKSLGDWDFLETVGAGSMGKVKLAKHRLTHEVCAIKIVNRATKALIHKESQQPPPRTQEEVLERERKLEKEISRDKRTIRESSLGQILYHPHICRLFEMCTMSNHFYMLFEYVSGGQLLDYIIQHGSLRERSARKFARGIASALQYLHLNNIVHRDLKIENIMISTSGEIKIIDFGLSNMFNPKKQLHTFCGSLYFAAPELLKACPYTGPEVDVWSFGVVLYVLVCGKVPFDDENSSVLHEKIKQGKVEYPQHLSIEVISLLSKMLVVDPSKRATLKQVVNHQWMQRGYDFPAPSYIPHRVPLTADALDPTVIQEMYRLEFVDNTSEAAAWLTKIVTSTDYQLLAKQFWANVSKGGAYEDPTRAFHPLISIYYLVEETLKRKLAKQQKRAAILAANQTPQALDVPLPASQPPAAMAGVLATPVIPGAPAAVNSPMKKNIINERPPTMSPSAALSPVKPKPTILIPPKLAIPEQAHTSPTTRKSNELALPPTADLDVVLSPTPQSRDYDQATAALQGRSGSPGAPSAPSTSEKSKFGTIFRRFSQKRHNQQSQQIPPPDANTGVKKTHARTVSEYVPPSRMTSYGSAPMPKVIPPAKQQSDLPALPADAADMVKNQQQQVQQNVERLTIADEDKPLPTLAVPKGRKMHPSARAKSVGHARRESLKFMRPPATGNMDQQVLNGNDDGFLENSDDGKSDDVANTDTIITTDDRELSDQEIINLASKAPVGSMPSIDYPRSLFLKGFFSVQTTSSKPLPIVRYKIISVLKKMNIEFKEVKGGFVCYHKQSYIPPTTQQPQQPVIVLSDASSMGSGNSGASAHKRNQTIPLQQGMHSRHNSIRRNPSMGKQQCQQPGITATPLAANTHERNLSVTSPNPRNSSIPEISSASLESWDQDDILTTSKAHNLNNADNAFDNSSEAKERPPLKFEIHIVKVRIVGLAGIHFKKVSGNTWMYKELASHILKELNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>C5DN49</accession>
    <name>C5DN49_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0G14146p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0G14146g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2016" name="Acta Crystallogr. F Struct. Biol. Commun." volume="72" first="799" last="803">
            <title>Crystal structures of a yeast 14-3-3 protein from Lachancea thermotolerans in the unliganded form and bound to a human lipid kinase PI4KB-derived peptide reveal high evolutionary conservation.</title>
            <authorList>
                <person name="Eisenreichova A."/>
                <person name="Klima M."/>
                <person name="Boura E."/>
            </authorList>
            <dbReference type="PubMed" id="27827352"/>
            <dbReference type="DOI" id="10.1107/s2053230x16015053"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS)</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2016-09" db="PDB data bank">
            <title>Lt 14-3-3 in complex with PI4KIIIB peptide.</title>
            <authorList>
                <person name="Boura E."/>
                <person name="Eisenreichova A."/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.58 ANGSTROMS)</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the 14-3-3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555647.1">
        <property type="nucleotide sequence ID" value="XM_002555601.1"/>
    </dbReference>
    <dbReference type="PDB" id="5LVZ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.95 A"/>
        <property type="chains" value="A=1-253"/>
    </dbReference>
    <dbReference type="PDB" id="5LX2">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.58 A"/>
        <property type="chains" value="A=1-253"/>
    </dbReference>
    <dbReference type="PDBsum" id="5LHO"/>
    <dbReference type="PDBsum" id="5LVZ"/>
    <dbReference type="PDBsum" id="5LX2"/>
    <dbReference type="AlphaFoldDB" id="C5DN49"/>
    <dbReference type="SMR" id="C5DN49"/>
    <dbReference type="FunCoup" id="C5DN49">
        <property type="interactions" value="1690"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DN49"/>
    <dbReference type="GeneID" id="8293931"/>
    <dbReference type="KEGG" id="lth:KLTH0G14146g"/>
    <dbReference type="eggNOG" id="KOG0841">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058290_0_0_1"/>
    <dbReference type="InParanoid" id="C5DN49"/>
    <dbReference type="OMA" id="KGCQLAR"/>
    <dbReference type="OrthoDB" id="10260625at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050815">
        <property type="term" value="F:phosphoserine residue binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030437">
        <property type="term" value="P:ascospore formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006031">
        <property type="term" value="P:chitin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031397">
        <property type="term" value="P:negative regulation of protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007124">
        <property type="term" value="P:pseudohyphal growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070873">
        <property type="term" value="P:regulation of glycogen metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001402">
        <property type="term" value="P:signal transduction involved in filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.190.20:FF:000002">
        <property type="entry name" value="14-3-3 protein epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.190.20">
        <property type="entry name" value="14-3-3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000308">
        <property type="entry name" value="14-3-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023409">
        <property type="entry name" value="14-3-3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036815">
        <property type="entry name" value="14-3-3_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023410">
        <property type="entry name" value="14-3-3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18860">
        <property type="entry name" value="14-3-3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00244">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000868">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00305">
        <property type="entry name" value="1433ZETA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00101">
        <property type="entry name" value="14_3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48445">
        <property type="entry name" value="14-3-3 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00796">
        <property type="entry name" value="1433_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00797">
        <property type="entry name" value="1433_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="7 8" id="KW-0002">3D-structure</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="14-3-3" evidence="4">
        <location>
            <begin position="5"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with phosphoserine on interacting protein" evidence="2">
        <location>
            <position position="58"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with phosphoserine on interacting protein" evidence="2">
        <location>
            <position position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000868-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR25210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="7">
        <source>
            <dbReference type="PDB" id="5LVZ"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="8">
        <source>
            <dbReference type="PDB" id="5LX2"/>
        </source>
    </evidence>
    <sequence length="253" mass="28570" checksum="C8023C8176282107" modified="2009-07-28" version="1">MSQSREDSVYLAKLAEQAERYEEMVDSMKAVASSGQELSVEERNLLSVAYKNVIGARRASWRIVSSIEQKEEAKDKSEHQVKLIRDYRSKIETELTKICDDILSVLDTHLIPSATTGESKVFYYKMKGDYHRYLAEFSSGEVRDKATNASLEAYKTASEIATTELPPTHPIRLGLALNFSVFYYEIQNSPDKACHLAKQAFDDAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDMSEAGQDEQQPAEGAQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DN61</accession>
    <name>C5DN61_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CCA tRNA nucleotidyltransferase, mitochondrial</fullName>
            <ecNumber evidence="7">2.7.7.72</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">CCA-adding enzyme</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">tRNA CCA-pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">tRNA adenylyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">tRNA nucleotidyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">KLTH0G14432g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Nucleotidyltransferase that catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs, which is necessary for the attachment of amino acids to the 3' terminus of tRNA molecules, using CTP and ATP as substrates. tRNA 3'-terminal CCA addition is required both for tRNA processing and repair. Also involved in tRNA surveillance by mediating tandem CCA addition to generate a CCACCA at the 3' terminus of unstable tRNAs. While stable tRNAs receive only 3'-terminal CCA, unstable tRNAs are marked with CCACCA and rapidly degraded. The structural flexibility of RNA controls the choice between CCA versus CCACCA addition: following the first CCA addition cycle, nucleotide-binding to the active site triggers a clockwise screw motion, producing torque on the RNA. This ejects stable RNAs, whereas unstable RNAs are refolded while bound to the enzyme and subjected to a second CCA catalytic cycle.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a tRNA precursor + 2 CTP + ATP = a tRNA with a 3' CCA end + 3 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:14433"/>
            <dbReference type="Rhea" id="RHEA-COMP:10465"/>
            <dbReference type="Rhea" id="RHEA-COMP:10468"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:74896"/>
            <dbReference type="ChEBI" id="CHEBI:83071"/>
            <dbReference type="EC" id="2.7.7.72"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 13">Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.72" evidence="7"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555659.1">
        <property type="nucleotide sequence ID" value="XM_002555613.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DN61"/>
    <dbReference type="FunCoup" id="C5DN61">
        <property type="interactions" value="154"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DN61"/>
    <dbReference type="GeneID" id="8293944"/>
    <dbReference type="KEGG" id="lth:KLTH0G14432g"/>
    <dbReference type="eggNOG" id="KOG2159">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019592_2_1_1"/>
    <dbReference type="InParanoid" id="C5DN61"/>
    <dbReference type="OMA" id="ASRFNCT"/>
    <dbReference type="OrthoDB" id="445712at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052929">
        <property type="term" value="F:ATP:3'-cytidine-cytidine-tRNA adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052927">
        <property type="term" value="F:CC tRNA cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004810">
        <property type="term" value="F:CCA tRNA nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001680">
        <property type="term" value="P:tRNA 3'-terminal CCA addition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05398">
        <property type="entry name" value="NT_ClassII-CCAase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.460.10:FF:000019">
        <property type="entry name" value="tRNA nucleotidyltransferase cca2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3090.10">
        <property type="entry name" value="cca-adding enzyme, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002646">
        <property type="entry name" value="PolA_pol_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032828">
        <property type="entry name" value="PolyA_RNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13734:SF5">
        <property type="entry name" value="CCA TRNA NUCLEOTIDYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13734">
        <property type="entry name" value="TRNA-NUCLEOTIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01743">
        <property type="entry name" value="PolyA_pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12627">
        <property type="entry name" value="PolyA_pol_RNAbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81891">
        <property type="entry name" value="Poly A polymerase C-terminal region-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Poly A polymerase head" evidence="14">
        <location>
            <begin position="50"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA nucleotidyltransferase/poly(A) polymerase RNA and SrmB- binding" evidence="15">
        <location>
            <begin position="219"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00050431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00056517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00066885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00072969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00076038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00077436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00080500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00082324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF12627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAR25222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="519" mass="59290" checksum="46665499A51E6C46" modified="2009-07-28" version="1">MLKKAASSLIQRNMVPKLKLTETEQQICRLLKDYTSEYNKSRALSEPLELRITGGWVRDKLLGFNSHDLDIAVNTMSGEQLALEINDFLRRNHESCGITPHSIHKIDKNPEKSKHLETATTKLFGIDVDFVNLRSEEYTTESRIPVVAFGTPKQDALRRDATLNALFYNIQNDEIEDFTGTGLEDLNKGILRTPLPPLQTFLDDPLRVLRLIRFASRLGFVIDPDTYKAMQDPEINKAFGIKISRERVGVEMQKILQGPNPLLGLSFIQKAGLDNVIFSWHSDSAIIHYNRENTDMSKAEKAYQELGRHIELVLEKLPVLLEAFPLLQEHWKNAPVFKQNFILGVIFAPFRGIKIVWNPQKLMNKETSISESIVKDGLKLGRNDAELIAKCVESHVEYNDMILRYESLSRSDIGLEIRSFNGDWELAHLVNLALAYCFDENSLGQYQNFYQFVFDQNLQNSHALKPLIDGKTLSKQLNIKPGRWMGGVVAEMVKWQMDNPGSDEKQVLEFVQTILPNYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>C5DN68</accession>
    <name>C5DN68_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0G14586p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0G14586g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SPT2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555666.1">
        <property type="nucleotide sequence ID" value="XM_002555620.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DN68"/>
    <dbReference type="FunCoup" id="C5DN68">
        <property type="interactions" value="485"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DN68"/>
    <dbReference type="GeneID" id="8293951"/>
    <dbReference type="KEGG" id="lth:KLTH0G14586g"/>
    <dbReference type="eggNOG" id="ENOG502QRG5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069667_1_0_1"/>
    <dbReference type="InParanoid" id="C5DN68"/>
    <dbReference type="OMA" id="IWAMFNR"/>
    <dbReference type="OrthoDB" id="4035998at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006360">
        <property type="term" value="P:transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013256">
        <property type="entry name" value="Chromatin_SPT2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22691:SF8">
        <property type="entry name" value="PROTEIN SPT2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22691">
        <property type="entry name" value="YEAST SPT2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08243">
        <property type="entry name" value="SPT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00784">
        <property type="entry name" value="SPT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="291"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="18"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="115"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="138"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="181"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="291"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR25229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="316" mass="36423" checksum="BE0DCA6389DC3229" modified="2009-07-28" version="1">MSFLAKLSQLRKKPATKPGEKTMDKSPKPVSGVILDPLRSESSLLPQKYVREEDPAIRRLKELRRKEQLKNAPKNKPGAPSSRKKKDENSATATETKFRRKVGQSTKPQRPVAPVRREPLKKLSFDELMKEAEKKASDPSTDSKAPSAQKATSSIAKPIKLNKPGFNKGARRTPAAAPVSKPRERKEPTVKLKQLSIPKSSIAQPGEKLRKKLDSIKKKRQGEAYGYEDEEDLDDFIEDDEEEEGFNRDEIWAIFNKGKKRRNFENDYDSDDMEANEMEILEEEERATKMARLEDKQEEEWLKRHEQEKRRKFVRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNH3</accession>
    <name>C5DNH3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">KLTH0G17028g</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="3"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555771.1">
        <property type="nucleotide sequence ID" value="XM_002555725.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNH3"/>
    <dbReference type="FunCoup" id="C5DNH3">
        <property type="interactions" value="608"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNH3"/>
    <dbReference type="GeneID" id="8294062"/>
    <dbReference type="KEGG" id="lth:KLTH0G17028g"/>
    <dbReference type="eggNOG" id="KOG0390">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000315_10_2_1"/>
    <dbReference type="InParanoid" id="C5DNH3"/>
    <dbReference type="OMA" id="DTHETYH"/>
    <dbReference type="OrthoDB" id="413460at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015616">
        <property type="term" value="F:DNA translocase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016817">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045003">
        <property type="term" value="P:double-strand break repair via synthesis-dependent strand annealing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000332">
        <property type="entry name" value="DNA repair and recombination protein RAD54-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.850">
        <property type="entry name" value="SWI2/SNF2 ATPases, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013967">
        <property type="entry name" value="Rad54_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050496">
        <property type="entry name" value="SNF2_RAD54_helicase_repair"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45629:SF7">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45629">
        <property type="entry name" value="SNF2/RAD54 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08658">
        <property type="entry name" value="Rad54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0347">Helicase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="15">
        <location>
            <begin position="425"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="16">
        <location>
            <begin position="762"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="52"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="242"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="373"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="52"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="269"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="383"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAR25334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1000" mass="111561" checksum="49FE245278B7018F" modified="2009-07-28" version="1">MPRDTCVTWQTRRVRFSLVSLCARAENKRVRRTSSLAPAAFFFSYPSTATTRTRTLTQSPTSTPAMAKRKLPDRPPNGIGAGQRPSLRPRALDSEAALATLTRPFKVPLKPLPTNARPVRSTRNKAVTYTYAPIQLPGDPENPDLALDSAGASADADITAARSHTKRIDALSARRLSRDPHRLQKIRDQLQRSFTVPIPGYVPRHNIPLSLGTKPKIVLGPRPLHDPTDEFAIVLYDPSVDGKTPELQDYQPNQSPPPSQLPPSSSKPTRPSATQTQQTGADSSQAGTPPPLRTHPKKLSNGLPNKTLRELLGERKLEKQFSSVPVVIDPLLVKILRPHQVEGVKFLYRCVTGLVMKDYLDQQSLLITADAATTKEPTPPPHTQSSSTESPASSPASTPSSATPPPAAQAPALQQCTVATEVSEIAQNRGAYGCIMADEMGLGKTLQCIALMWTLLRQGPQGKPTIDKCIIVCPSSLVNNWANEIVKWLGRGTLASLPIDGKKSSLSNGTVAQAVRSWALAQGRSVVKPVLIISYETLRRNVEHLRHCDVGLLLADEGHRLKNADSQTFTSLNSIRCPRRVILSGTPIQNDLSEYFALLNFSNPGLLGSRLEFRKNFELPILRGRDADAMDEDVKKGDERLQALSTIVSKFIIRRTNDILSKYLPCKYEHVIFVNLKPFQRSLYEHMLKSRDIKLLVKDAKHTQPLKHIGVLKKLCNHPDLLRLPDDIEGSEDLMPEDYQSSTVSKRGRSELQTWHSGKFSILGRFLHKIKTESDDKIVIISNYTQTLDLIEKMCRSSTYPVVRLDGTMTINKRQKLVDRFNDPEGQEFIFLLSSKAGGCGINLIGANRLILMDPDWNPAADQQALARVWRDGQKKDCFIYRFICTGSIEEKIYQRQSMKMSLSSCVVDEKEDVERLFSAGNLKQLFELRPDTNCDTHETYHCKRCKNGKQMVKSNAMLYGDATTWNHLNHDALAKTNDHLLCNEFSFDDISYVFQYISH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-04-02" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNI5</accession>
    <name>C5DNI5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0G17336p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0G17336g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555783.1">
        <property type="nucleotide sequence ID" value="XM_002555737.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNI5"/>
    <dbReference type="STRING" id="559295.C5DNI5"/>
    <dbReference type="GeneID" id="8294076"/>
    <dbReference type="KEGG" id="lth:KLTH0G17336g"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_562677_0_0_1"/>
    <dbReference type="InParanoid" id="C5DNI5"/>
    <dbReference type="OrthoDB" id="6910977at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="417"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="248"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR25346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="485" mass="54335" checksum="D91C505A7835BFA3" modified="2009-07-28" version="1">MLVMSQLEPAAHEEILYFGNSWEMHRQPECLESSPSLAESAPRESLWPFDLLDQDTACPPTMPSPPSLGPPSAAVRQAVPARPTMVKANSFSEGVHDFDDMSHFLSFAFVPSHASFQHQYPHQHMHSYTHPHPQPHSQAISQAIPQPRLHAHTHVYQDQDSGADSAPDYHFEPVVTRHSFAGHAASAGHIASAGHIASTGYTASAGHAASAGNIASAGRAATAESDYEGVPLAWQSAPSQTFFESFAPESLSPETRIRSAEPDSSPPALPSSAQSTYFSDAPADTASEPSSDLHDRFYQAFNNPTQRPTSSKHESVYQQLIDDNPLMEALSKRVKRGYYRCAHCPKMFSNVLEYAKHIDEFEIQRDYKCPFVLCPWKILGLPRRPELRRHCAIQHKMEIPKELKSTLKLGETDFPIMECTSPYCDKKFYRRDSYARHVAMVHDKADSRFNKRLVKVLAECPYEQDAFQHRGYIMGEMMKTKKKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNJ5</accession>
    <name>C5DNJ5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0G17556p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0G17556g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555793.1">
        <property type="nucleotide sequence ID" value="XM_002555747.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNJ5"/>
    <dbReference type="FunCoup" id="C5DNJ5">
        <property type="interactions" value="483"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNJ5"/>
    <dbReference type="GeneID" id="8294086"/>
    <dbReference type="KEGG" id="lth:KLTH0G17556g"/>
    <dbReference type="eggNOG" id="KOG3319">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072117_2_1_1"/>
    <dbReference type="InParanoid" id="C5DNJ5"/>
    <dbReference type="OMA" id="STHYTHF"/>
    <dbReference type="OrthoDB" id="1932233at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007203">
        <property type="entry name" value="ORMDL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12665">
        <property type="entry name" value="ORMDL PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04061">
        <property type="entry name" value="ORMDL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018147">
        <property type="entry name" value="ORMDL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="7"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR25356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="201" mass="22804" checksum="1D227D146BD95567" modified="2009-07-28" version="1">MSDRPQLKSSPSGGSSNALLEPVKDRRRRRSSSLISHVEPETLEEENDQQMPNMNASWVDQRGAWAVHIVIIMLLKGFYNLLPGITNEWSWTLTNTTYVIGSYIMFHLVKGTPFDFNGGAYDNLTMWEQINDGVLYTPARKFLILVPIALFLVSTHYTRYDIQLFLLNFSLTTLVAVVPKLPVTHRLRITIPGLTSPAQIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNM7</accession>
    <name>C5DNM7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0G18392p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0G18392g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555825.1">
        <property type="nucleotide sequence ID" value="XM_002555779.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNM7"/>
    <dbReference type="GeneID" id="8294120"/>
    <dbReference type="KEGG" id="lth:KLTH0G18392g"/>
    <dbReference type="HOGENOM" id="CLU_897340_0_0_1"/>
    <dbReference type="InParanoid" id="C5DNM7"/>
    <dbReference type="OMA" id="KSITRNQ"/>
    <dbReference type="OrthoDB" id="4036644at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR25388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="310" mass="33914" checksum="5CB7C53A19FAEF40" modified="2009-07-28" version="1">MQALSMALPLQRIDSSVYDFQALSDGNYLPLDAASFSSGMEHDDEPECEPDHAYAPAPALPAPALGPGSEHDHVRHRTQFTRAHDSLLLHVVLMNGHRLACDPKSKSRRLFWHHVSTEMATSFGVHKNKRQCRDRFKLLFQRATSQNQFESDTARAPPTTELDRLLRTCFHTFRFTEAKEIALAHAGPAQHLHVPPTAPATAPATVSPLPSSGMATMINTPVSQPEFCEKATPAPTTAPASASAVAQGSAAWVPSPSWETQTVLASISSLQRQINQLSSQIDALSNVVSMSRMQPRLYQPMYTGPVNDSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNP2</accession>
    <name>C5DNP2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">CBK1 kinase activator protein MOB2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0G18722g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="10">Interacts with protein kinase CBK1 to form the RAM CBK1-MOB2 kinase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MOB1/phocein family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555840.1">
        <property type="nucleotide sequence ID" value="XM_002555794.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNP2"/>
    <dbReference type="FunCoup" id="C5DNP2">
        <property type="interactions" value="157"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNP2"/>
    <dbReference type="GeneID" id="8294135"/>
    <dbReference type="KEGG" id="lth:KLTH0G18722g"/>
    <dbReference type="eggNOG" id="KOG0440">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038321_2_1_1"/>
    <dbReference type="InParanoid" id="C5DNP2"/>
    <dbReference type="OMA" id="CNHSSER"/>
    <dbReference type="OrthoDB" id="10261121at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051286">
        <property type="term" value="C:cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030295">
        <property type="term" value="F:protein kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007163">
        <property type="term" value="P:establishment or maintenance of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000920">
        <property type="term" value="P:septum digestion after cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.30:FF:000004">
        <property type="entry name" value="CBK1 kinase activator protein MOB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.30">
        <property type="entry name" value="MOB kinase activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005301">
        <property type="entry name" value="MOB_kinase_act_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036703">
        <property type="entry name" value="MOB_kinase_act_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22599">
        <property type="entry name" value="MPS ONE BINDER KINASE ACTIVATOR-LIKE MOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03637">
        <property type="entry name" value="Mob1_phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01388">
        <property type="entry name" value="Mob1_phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101152">
        <property type="entry name" value="Mob1/phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="13"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="19"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="55"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00062973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00068888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR605301-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR25403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="278" mass="32158" checksum="504B368E2CD6F2EE" modified="2009-07-28" version="1">MSFFNSFKGFTRSSKKTKNQNVGTTANSVNSVYPNAHNNSSKLLSRQVHSPVRQAPSSHNTNSFQQQQQQQQVHAIPSERVETQQSLFLSEPFVRTALVKGSFKTIVQLPKYVDLGEWVALNVFEFYTNLNQFYGIIAEYVTPDVYPTMNAGPHTDYMWLDANSRQVSLPAGQYIDLALTWISNKVSDQTLFPTQNGYPFPQNFVQDMQRIMVQMFRIFAHMYHHHFDKIIHLSLEAHWNSLFAHFISFAKEYNLVDRKEMYPLGPLIENLERQGKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNQ0</accession>
    <name>C5DNQ0_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Mitochondrial 15S rRNA processing factor CCM1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0G18898g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Regulates mitochondrial small subunit maturation by controlling 15S rRNA 5'-end processing. Localizes to the 5' precursor of the 15S rRNA in a position that is subsequently occupied by mS47 in the mature yeast mtSSU. Uses structure and sequence-specific RNA recognition, binding to a single-stranded region of the precursor and specifically recognizing bases -6 to -1. The exchange of Ccm1 for mS47 is coupled to the irreversible removal of precursor rRNA that is accompanied by conformational changes of the mitoribosomal proteins uS5m and mS26. These conformational changes signal completion of 5'-end rRNA processing through protection of the mature 5'-end of the 15S rRNA and stabilization of mS47. The removal of the 5' precursor together with the dissociation of Ccm1 may be catalyzed by the 5'-3' exoribonuclease Pet127. Involved in the specific removal of group I introns in mitochondrial encoded transcripts.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Binds to mitochondrial small subunit 15S rRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CCM1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555848.1">
        <property type="nucleotide sequence ID" value="XM_002555802.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNQ0"/>
    <dbReference type="FunCoup" id="C5DNQ0">
        <property type="interactions" value="106"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNQ0"/>
    <dbReference type="GeneID" id="8294143"/>
    <dbReference type="KEGG" id="lth:KLTH0G18898g"/>
    <dbReference type="eggNOG" id="KOG4197">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_306786_0_0_1"/>
    <dbReference type="InParanoid" id="C5DNQ0"/>
    <dbReference type="OMA" id="HGYNLIH"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057027">
        <property type="entry name" value="TPR_mt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47936:SF1">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN GUN1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47936">
        <property type="entry name" value="PPR_LONG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23276">
        <property type="entry name" value="TPR_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Pentatricopeptide repeat-containing protein-mitochondrial" evidence="10">
        <location>
            <begin position="361"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="9">
        <location>
            <begin position="396"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR25411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="941" mass="109345" checksum="13FE3C4253B5F8BF" modified="2009-07-28" version="1">MDGVRTSLLRHMQCWHSHCQSRYSSTVTSCLPRKIKDIFDDGKAEPLQTRQLNRILSFVRESRPHSSQRSALIKCLHRRKAHKDVVLVAKSALFDFNHNLQPDVSAYELKKYFSSLLSIGKSIQMEQAMFQLIKQFSLTNKSMVTNVINATFVQSTTTESVSQALLTWVRFNNYLHGHADFTNYEQYKYILKTLLFFLRAEKPEPHVLAETLNFVNSTQGPLITSQFASTLIYLSCYNRDFSVTESLWNYKREKEYPIIKSDLTSICKAYAHFEKFEQIQAAWSEFPDAQDDAQFDYLLIAHAKLQDWGNLQNQFNALFGIGDLPSINHYGIVMFSIAHLGEREMVEKLYNQLLRRKMIPTLPVLQSLLQVCYKTGDMNGCFQHFELFKKYGIKPTPSTCNIMLRIFRNLSDIDGSLRFLKRMTENGIEVSEKHFSTIINTCAKTTNYAIAGELFQVMKDHYDIKPTGSSVAALMHVYIKSDLPQEALKVFKEYRYTKAPLESEISIYNKAVEAYMRMNRSDMSEKIFEQILSKNLPADSEFYRVMIKHSALYMKDFETAENILTQLLSHSKLKATPTHFEALMGAYDKYSNRRGVLDLYQRMLENNVPVNSKVLFYLIKATFKTRLRSRENLDEAISLVDDIMSQAANRTLDITFEKLHPSVMAWPMRAAAKYHSPLRALELMNKYNDMFFDKTDSAESRLVVIRSLMVLSAEVHQWDDFQKFFERYMNRIQYYQNLPSATVRNRNFASLLRGVLTYKIKHLVATNRATEIPALLEDVSAKNFVIDNEAWNEAITSMFADMRTIGKGLQAVDTKLIHGFNLIHKYRLLRNKAASNTSTDRGSWFLEKKRQAPSSYQPKLYLKSNVLEKTFESMDNYLSTISNVESELKDLTSRFKYYMKRYLMHPRNKIPGWNEIEVKHAAYFQKLRDSKRAISPEELSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5E207</accession>
    <name>C5E207_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0H01210p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0H01210g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the prefoldin subunit beta family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555930.1">
        <property type="nucleotide sequence ID" value="XM_002555884.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E207"/>
    <dbReference type="FunCoup" id="C5E207">
        <property type="interactions" value="871"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E207"/>
    <dbReference type="GeneID" id="8294243"/>
    <dbReference type="KEGG" id="lth:KLTH0H01210g"/>
    <dbReference type="eggNOG" id="KOG3478">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125172_1_1_1"/>
    <dbReference type="InParanoid" id="C5E207"/>
    <dbReference type="OMA" id="VQTEFAQ"/>
    <dbReference type="OrthoDB" id="248120at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051131">
        <property type="term" value="P:chaperone-mediated protein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23161">
        <property type="entry name" value="Prefoldin_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.370:FF:000003">
        <property type="entry name" value="Prefoldin subunit 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002777">
        <property type="entry name" value="PFD_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21431">
        <property type="entry name" value="PREFOLDIN SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21431:SF0">
        <property type="entry name" value="PREFOLDIN SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01920">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="12"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR30068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="113" mass="13139" checksum="7561053EF2DDF4F7" modified="2009-07-28" version="1">MSSSAQDLTMNYNTSQHELEELVIARQKLETQLQENKIVQDEFDGLREETQVYKLTGNVLLPVEQFEAKSNVSKRLEFITAEINRCEENIKTKQGLLEKLRAELLQTQQARQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5E267</accession>
    <name>C5E267_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ATPase inhibitor, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0H02530g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Inhibits the enzyme activity of ATPase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the ATPase inhibitor family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555990.1">
        <property type="nucleotide sequence ID" value="XM_002555944.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E267"/>
    <dbReference type="FunCoup" id="C5E267">
        <property type="interactions" value="98"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E267"/>
    <dbReference type="GeneID" id="8294303"/>
    <dbReference type="KEGG" id="lth:KLTH0H02530g"/>
    <dbReference type="eggNOG" id="ENOG502SCJG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_145563_4_0_1"/>
    <dbReference type="InParanoid" id="C5E267"/>
    <dbReference type="OMA" id="RCISRNT"/>
    <dbReference type="OrthoDB" id="5532350at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042030">
        <property type="term" value="F:ATPase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.500">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007648">
        <property type="entry name" value="ATPase_inhibitor_mt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04568">
        <property type="entry name" value="IATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="62"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU368087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR30128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="98" mass="11153" checksum="A50E8F9EAE49E662" modified="2009-07-28" version="1">MLSQSARASLKKSLKLQLPAMPAMPAVGGRFYSEGSVGSPRGEGADDSFVRRERAQEDYFIRQHEKEQLEKLRQQVKQQQQKIEHLEGRLGDKLKEGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5E278</accession>
    <name>C5E278_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0H02772p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0H02772g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556001.1">
        <property type="nucleotide sequence ID" value="XM_002555955.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E278"/>
    <dbReference type="FunCoup" id="C5E278">
        <property type="interactions" value="918"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E278"/>
    <dbReference type="GeneID" id="8294314"/>
    <dbReference type="KEGG" id="lth:KLTH0H02772g"/>
    <dbReference type="eggNOG" id="KOG0290">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013694_1_1_1"/>
    <dbReference type="InParanoid" id="C5E278"/>
    <dbReference type="OMA" id="TIAMDAC"/>
    <dbReference type="OrthoDB" id="1284551at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045159">
        <property type="entry name" value="DCAF7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19919">
        <property type="entry name" value="WD REPEAT CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="205"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="322"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="253"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="257"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR30139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="420" mass="46280" checksum="639E32EBF076BC96" modified="2009-07-28" version="1">MDTYKAGKRSSISFGSYQRPVVSFNQNELPYYAPRQLYSAQQSAQQPDVVYPCRYEAHFPLYAMDWSNEDYVAVGSYKEDTFNKLQILHSSDLVSWEKAGEENCVFPISRVQWCPNGNSQQLATCSDSLRLWDYSDFVLQERLNLSFCRYNKSGGQGTVAALGQLPPVSSFHWNAVDPNLIISSSIDTTCTVWDLQATNYVKTQLIAHDSEVFDVKFLTQSTQLFASCGGDGSVRVFDLRSLAHSTIIYEPSSQPTAGSSGDSSAASGGSGSGSNSASSGSNALLRLEPSPFDPNVVATFAHDSNSVLILDMRYPGAAILTLEGHVGAVNQIQWHPSRHNVLLTCGDDCQALLWDLNTHLSTPASNTVSSKWNSSKVARCIDTPQEAYSDANYEINNIVWRPQGNWFGCNLGRHFQSVRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5E282</accession>
    <name>C5E282_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">UDP-galactose transporter homolog 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0H02860g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleotide-sugar transporter family. SLC35B subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556005.1">
        <property type="nucleotide sequence ID" value="XM_002555959.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E282"/>
    <dbReference type="FunCoup" id="C5E282">
        <property type="interactions" value="481"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E282"/>
    <dbReference type="GeneID" id="8294318"/>
    <dbReference type="KEGG" id="lth:KLTH0H02860g"/>
    <dbReference type="eggNOG" id="KOG1581">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036019_0_2_1"/>
    <dbReference type="InParanoid" id="C5E282"/>
    <dbReference type="OMA" id="CGAIGQV"/>
    <dbReference type="OrthoDB" id="1601at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005459">
        <property type="term" value="F:UDP-galactose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005460">
        <property type="term" value="F:UDP-glucose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013657">
        <property type="entry name" value="SCL35B1-4/HUT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10778">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10778:SF10">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08449">
        <property type="entry name" value="UAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="40"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="137"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="205"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="245"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="274"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="301"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR30143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="330" mass="36866" checksum="B7CBB1DDF5B4286E" modified="2009-07-28" version="1">MGSQILLAVCVLGIYATFLTWSLVYEPLTTKVWPNSAEKFHVPCVVSLVQASVAAFTGYAYLRREKTGYRTKEFMKDNYKDLLLISFTQSTSSPLATYSLQYVDFLTYMLAKSCKLIPILAVHLLLYHTKITNNKKAVAIAVTFGVTLFNLGSAKRSSSTRGQNVSYVNGFAPLVLSLLLDGFTNATQDTLLKRNRTSNKKPITGGHLMLGLNMCIVFWNFFYLLLIDPEQALKAKNMITEDPEIAYYLLTYAACGALGQICIFYTLQEYGSLVLVMVTVTRKMFSMILSILVYGHHVTRMQWLGISIVFGGITAEALLKRRGASKLKTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2H7</accession>
    <name>C5E2H7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0H05038p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0H05038g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the WD repeat WDR6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556100.1">
        <property type="nucleotide sequence ID" value="XM_002556054.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2H7"/>
    <dbReference type="FunCoup" id="C5E2H7">
        <property type="interactions" value="695"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2H7"/>
    <dbReference type="GeneID" id="8294414"/>
    <dbReference type="KEGG" id="lth:KLTH0H05038g"/>
    <dbReference type="eggNOG" id="KOG0974">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002615_0_1_1"/>
    <dbReference type="InParanoid" id="C5E2H7"/>
    <dbReference type="OMA" id="GGAHRQW"/>
    <dbReference type="OrthoDB" id="66881at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051973">
        <property type="entry name" value="tRNA_Anticodon_Mtase-Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14344">
        <property type="entry name" value="WD REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14344:SF3">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="3 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="177"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="219"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR30238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="995" mass="112751" checksum="BD847E996B65B5B1" modified="2009-07-28" version="1">MSQLRQLSHTGPSVCVKFHGKYVLAGYGVFVHVYNYESGELVSISRVFHRNKVHGLAIREKSVLLYGGRSVSLLDIDYLWGNADNTRFEKLCHEWVVSGEHSMDGSSVFLLTSYNKVLCCDLSFRVLQTKAVYGERSILYSGTITVLNQDKVLVNAGTVMDGVLVWDLKTEKTIHHFTDHEGSIFYVAASIKGRYAASCSDDRSIKLWDLHSGHLVSTGWGHTARIWNLKFYDNDSKLISVSEDCTCRVWEISERGTLIQKSIHELHQTKSIWGVDVNDEKMIAVTSGNDGRIKITDLNARTKSDSDSRIFSLDDISKHGVKLRPNEIIKGFYWLRFGLLAMTSEGQVLKFDQKAEMWTSVMTDSRFTSYSATSGVQEFDMVIFSNSRCELLILKFSEDGNEILENKQLQINELTKSTNCLVRGYNSKLFLVMESPNPKDPFFCLRMDASNLQHEERLYFKKPKNFTASSFAFYENFAIFGSRFSTLAIFDLENCEREATVLRKILPGDTITSIDFVEKNSESDCLFSVSDRDGFYCFISINFLKRSHKVLLQNRIPKGFLEGAHYNAQGDFITYGFKSNLFYVFNETKQYEVFSELCGGAHRQWRYLPNFGLDEFIFIYIKASSINFRKIHKAAYPEILRNGLHGREIRDLCVRTEPLHLGKTVFCSASEDTTIKLNVLNVKDGETKTVWTFRKHTSGLQRCKFINNKFMISSAAREELYLWELTDTFMSNPYMTIICALPSSSEIPDLRIMDFACKAIEGTEDFILTTVYSDSAIKVWLYSSDENTFKLLISDKYQTCCLLNVALVTLDTELILVVSPTDGHLVCWNLTKYIPYVIQNNKLVKVLEGFQPQIHLPDISERIAVHSAGIKSFEFKIIAENRFFVYTGGDDNAVAISEFVLDKSHKTIQGCVRSFISAGAASTITSVKLIRRDMTLLTASVDQVVRYYDVSAGDLVLKNKFYTTNADTGCIDTLSSATGSIALIGGVGFSVWTLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2I2</accession>
    <name>C5E2I2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="7">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0H05148g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the PPP phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="7"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556105.1">
        <property type="nucleotide sequence ID" value="XM_002556059.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2I2"/>
    <dbReference type="FunCoup" id="C5E2I2">
        <property type="interactions" value="184"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2I2"/>
    <dbReference type="GeneID" id="8294419"/>
    <dbReference type="KEGG" id="lth:KLTH0H05148g"/>
    <dbReference type="eggNOG" id="KOG0374">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004962_4_1_1"/>
    <dbReference type="InParanoid" id="C5E2I2"/>
    <dbReference type="OMA" id="EVQLICA"/>
    <dbReference type="OrthoDB" id="1930084at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007346">
        <property type="term" value="P:regulation of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000026">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050341">
        <property type="entry name" value="PP1_catalytic_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031675">
        <property type="entry name" value="STPPase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668">
        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668:SF423">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE PPQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16891">
        <property type="entry name" value="STPPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0464">Manganese</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="9">
        <location>
            <begin position="325"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="99"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="15"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="101"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="118"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR30243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="515" mass="56824" checksum="BCCD2C9E85EB17DB" modified="2009-07-28" version="1">MGNSPSKSNYAIPSTKTASATTQGLTTTGDDLNSNEPNDPDDSRSLPTQVIKIHKVAPGEPKNNVIPSAAASGRGARRKSPAAYDLEIDMSFAKALSATTSSQGSSSGSSSGSSSGSHDSNMTPPSYSTDSSFLNYSFPDEPQTPGSANGAPVAASLPPIKEEMAVTASLVRRNRLQQRGHPHIPHDRNAAPPVILKRYAHDLQSTEHLDIDDVIEKLLKIGETRYYQAKEFPFSSWKVQLICARARELFLEQPSLLRLQAPIKIVGDVHGQFTDLMRILKLSGVPPNTSYLFLGDYVDRGKQSLETMLLLLCYKIKFPDRFFMLRGNHESANVTKMYGFYDECKRRLSTKCWKMFVDVFNTLPLAAVVQDKIFCVHGGISPDLKDLGQIERIARPTDIPEEGLFTDLLWSDPDPQVSDWSVNDRGVSVTFGKRNVLDFCSRFNFDLIIRGHMVVEDGYEFFARKKFVTVFSAPNYCGEFQNWGAVMSVTTGLMCSFELLKPHGAKGGAAHKHKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2I3</accession>
    <name>C5E2I3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Nuclear cap-binding protein subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">20 kDa nuclear cap-binding protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0H05214g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the RRM NCBP2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556106.1">
        <property type="nucleotide sequence ID" value="XM_002556060.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2I3"/>
    <dbReference type="FunCoup" id="C5E2I3">
        <property type="interactions" value="953"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2I3"/>
    <dbReference type="GeneID" id="8294421"/>
    <dbReference type="KEGG" id="lth:KLTH0H05214g"/>
    <dbReference type="eggNOG" id="KOG0121">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070952_1_0_1"/>
    <dbReference type="InParanoid" id="C5E2I3"/>
    <dbReference type="OMA" id="DELRWDH"/>
    <dbReference type="OrthoDB" id="201398at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005846">
        <property type="term" value="C:nuclear cap binding complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000339">
        <property type="term" value="F:RNA cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12240">
        <property type="entry name" value="RRM_NCBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000538">
        <property type="entry name" value="Nuclear cap-binding protein subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027157">
        <property type="entry name" value="NCBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034148">
        <property type="entry name" value="NCBP2_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18847">
        <property type="entry name" value="20 KD NUCLEAR CAP BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18847:SF0">
        <property type="entry name" value="NUCLEAR CAP-BINDING PROTEIN SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8 11" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RRM" evidence="13">
        <location>
            <begin position="46"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="174"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="181"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU364036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR30244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="200" mass="22774" checksum="BE8FFEBA9FA4AE2E" modified="2009-07-28" version="1">MSLAEFDEINYDHSASRLDVPSNYLMKKARRNPNGLEDLRRSMRSATIYVGNLSFYTSEEQIYELFSKSGVIKRIIMGLDRFKFTPCGFCFVIYNTPEEALDAVKYLSGTKLDDRNISLDLDPGFEDGRQFGRGKSGGQVSDELRFEFDASRGGFTVPLVDRIGFSNTHNFGARRVRDTYVPEEPEDDEEEPDTYVPGED</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2J1</accession>
    <name>C5E2J1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0H05412p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0H05412g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556114.1">
        <property type="nucleotide sequence ID" value="XM_002556068.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2J1"/>
    <dbReference type="GeneID" id="8294429"/>
    <dbReference type="KEGG" id="lth:KLTH0H05412g"/>
    <dbReference type="HOGENOM" id="CLU_983772_0_0_1"/>
    <dbReference type="InParanoid" id="C5E2J1"/>
    <dbReference type="OrthoDB" id="4035148at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950720" evidence="2">
        <location>
            <begin position="21"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="233"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR30252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="283" mass="30647" checksum="35A142EF59E125BC" modified="2009-07-28" version="1">MKIQTLFCVGLLLAFATSSASFWEGLSQLTHKKTSFLKWDFNVDRLANNVGRLTGDTHFLAVTDAVVRNAERQMLNLFIQNPAVGITKDVNRLCREIESSSARLVISLDHPAWEPARQQVTQYLEGRSREFGLRLASHIRGDARSPSAGLVDLLASFSKSLQNAVSLVENPSKSSDAFEEFTFASRMDDLTVGRIVGEGIAEKIRDKVVGGDTHAALTDIANIFCDFGTLMTAYVTGLAICTAGLGAALCGVSIIICGGGTIIYICRLLQNLDKVFKTLGVGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2N8</accession>
    <name>C5E2N8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Oxalate--CoA ligase</fullName>
            <ecNumber evidence="5">6.2.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Acyl-activating enzyme 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Oxalyl-CoA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0H06490g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.8" evidence="5"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556161.1">
        <property type="nucleotide sequence ID" value="XM_002556115.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2N8"/>
    <dbReference type="FunCoup" id="C5E2N8">
        <property type="interactions" value="647"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2N8"/>
    <dbReference type="GeneID" id="8294477"/>
    <dbReference type="KEGG" id="lth:KLTH0H06490g"/>
    <dbReference type="eggNOG" id="KOG1176">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_0_1"/>
    <dbReference type="InParanoid" id="C5E2N8"/>
    <dbReference type="OMA" id="TFRGYYR"/>
    <dbReference type="OrthoDB" id="3633556at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031956">
        <property type="term" value="F:medium-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050203">
        <property type="term" value="F:oxalate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033611">
        <property type="term" value="P:oxalate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05926">
        <property type="entry name" value="FACL_fum10p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000007">
        <property type="entry name" value="4-coumarate--CoA ligase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000039">
        <property type="entry name" value="Peroxisomal-coenzyme A synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045310">
        <property type="entry name" value="Pcs60-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43201">
        <property type="entry name" value="ACYL-COA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43201:SF5">
        <property type="entry name" value="MEDIUM-CHAIN ACYL-COA LIGASE ACSF2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="9">
        <location>
            <begin position="19"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="10">
        <location>
            <begin position="448"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00066722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00067955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00078960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00083427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR30299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="539" mass="60008" checksum="60A44B0F173134A5" modified="2009-07-28" version="1">MTQDTRGVASFNESFKVTDNVALVVPETGTQVTYRDLSHMTGHFQTLFQSPDSPLYKLIGRQSAIAISMANGLEFVTAFLGITTDSKIAAPLNPNYKAEELNFYLNDLKTKVICVPQGTVQAGSNAEILKSASKFGCFIVELYFDKERFRVEYDVYSPQESYKRKVYSSINHAVFHNHSDKFPGFARSSDVALILHTSGTTSAPKTVPLLHLNIARSMRNIAATYKLSPADRSYVVMPLFHVHGLIGVLLSTFWAQGSVVVPPRFSVKKFWNDFITWNCNWFSCVPTISMIMLNSPKPNPLPHIRFIRSCSSALAPSTFAKLESEFQAPVLEAYAMTEASHQMTSNNLPPGKRKPGTVGQPQGVEVFILDDKDNVLPQGQQGEVSIRGENVTLGYAHNEKANRENFTKRENYFRTGDQGYFDPEGFLVLTGRIKELINRGGEKISPIELDGVMLSHPKVDEAVAFGVSDEKYGQIVNAAVVLKLGQKLDYKELTDYMRSKVASFKVPTKIFFVDKLPKTATGKIQRRIIAEVFAGKSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2Y9</accession>
    <name>C5E2Y9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 11">V-type proton ATPase subunit F</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0H08932g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10 11">Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'', d, e, f and VOA1).</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">V-ATPase is a heteromultimeric enzyme made up of two complexes: the ATP-hydrolytic V1 complex and the proton translocation V0 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Vacuole membrane</location>
            <topology evidence="8">Peripheral membrane protein</topology>
            <orientation evidence="8">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the V-ATPase F subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556262.1">
        <property type="nucleotide sequence ID" value="XM_002556216.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2Y9"/>
    <dbReference type="FunCoup" id="C5E2Y9">
        <property type="interactions" value="762"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2Y9"/>
    <dbReference type="GeneID" id="8294587"/>
    <dbReference type="KEGG" id="lth:KLTH0H08932g"/>
    <dbReference type="eggNOG" id="KOG3432">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_135754_0_0_1"/>
    <dbReference type="InParanoid" id="C5E2Y9"/>
    <dbReference type="OMA" id="IIICQHI"/>
    <dbReference type="OrthoDB" id="10261947at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033180">
        <property type="term" value="C:proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10580:FF:000002">
        <property type="entry name" value="V-type proton ATPase subunit F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10580">
        <property type="entry name" value="ATPase, V1 complex, subunit F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008218">
        <property type="entry name" value="ATPase_V1-cplx_f_g_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005772">
        <property type="entry name" value="ATPase_V1-cplx_fsu_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036906">
        <property type="entry name" value="ATPase_V1_fsu_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01101">
        <property type="entry name" value="V_ATP_synt_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13861:SF2">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13861">
        <property type="entry name" value="VACUOLAR ATP SYNTHASE SUBUNIT F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01990">
        <property type="entry name" value="ATP-synt_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015945">
        <property type="entry name" value="ATPase_V1_F_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159468">
        <property type="entry name" value="AtpF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="6 11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <keyword evidence="4" id="KW-0926">Vacuole</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00046254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR015945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR30400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="118" mass="13274" checksum="D5DD77A07E4CA050" modified="2009-07-28" version="1">MSENRSLIAIIGDEDTCTGLLLAGVGQISPSSQEKNFFMYQEGKTTREEILGAFDKFTQERDDIAILLINQHVAEKIRGQVDSYTAAFPAVLEIPSKEHPYDPEKDSVLKRVRRLFGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>C5E311</accession>
    <name>C5E311_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">KLTH0H09416p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0H09416g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556284.1">
        <property type="nucleotide sequence ID" value="XM_002556238.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5E311">
        <property type="interactions" value="310"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E311"/>
    <dbReference type="GeneID" id="8294609"/>
    <dbReference type="KEGG" id="lth:KLTH0H09416g"/>
    <dbReference type="eggNOG" id="KOG3417">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002171_1_0_1"/>
    <dbReference type="InParanoid" id="C5E311"/>
    <dbReference type="OMA" id="SEHEYSL"/>
    <dbReference type="OrthoDB" id="546434at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00155">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06224">
        <property type="entry name" value="REM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11883">
        <property type="entry name" value="SH3_Sdc25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008937">
        <property type="entry name" value="Ras-like_GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000651">
        <property type="entry name" value="Ras-like_Gua-exchang_fac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019804">
        <property type="entry name" value="Ras_G-nucl-exch_fac_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113:SF368">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00618">
        <property type="entry name" value="RasGEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00229">
        <property type="entry name" value="RasGEFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00720">
        <property type="entry name" value="RASGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50212">
        <property type="entry name" value="RASGEF_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3 5" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="8">
        <location>
            <begin position="22"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="N-terminal Ras-GEF" evidence="10">
        <location>
            <begin position="1063"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GEF" evidence="9">
        <location>
            <begin position="1249"/>
            <end position="1486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="99"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="170"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="315"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="376"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1488"/>
            <end position="1528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="108"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="189"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="220"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="315"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="335"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="376"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="400"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1509"/>
            <end position="1528"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR30422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1528" mass="171539" checksum="6E80738894B1DEBD" modified="2009-07-28" version="1">MSLSRVSSHDEQTPAARAGSTRPVDVVVATCDFTPTKKAQLRLSAGDVVYVLGKNESGWWDGVTVCGRSPQRVARGWFPHNFTRSYREHKRGGLAAKCGAASSVAGTRSNRSGGSSRRSSLAAPPMPGHMDFGSSMRLGTVSEPNLSPTDSHAPHLKQDYRFDMRSNLAGQAGSRRPSMASGWSAGANSLSKSPSHSSNEQQSHGSQSQPQQVDPLQKSTADERRRRSHVAETDKMNVLGTDEVQMIFSNIHDNSPPIWTPVPTTEGKILYYNRDYDIYCSGLPLLQSPELNIKSVFSDHDWFVDIQERALTHNKKVLDKKEEPAGANGAATGPMSANTQSTSQNTRNHSGSRRPSVDEKSKNYLAATIISDSAGTGSRNTKINALKSDNLSPKRVAGDDTNTNTNSDSKAGIKTATENENDTEVAASDPGWWQKNPKHTLLAKEELFYHHRMDLRSWTEMRDTTLYFARKAYASFFQNDYHQFNRNFEVTAKFSIYYHNACRLLKGELIKNNVKREVRRILRQMTDAVSAISINGNLFFSSPQRFDISFSPSVQQQQLQQRHKSSVGSTGTAIQDPLRVSISTLNPLQPGDLQKLSISSNDILINEESQSLETKSKNDRDDRFGSVLSSYTIQAHSTAFDENSKISIQALFQTIDAQFSRFMICVRNLDALMKTKCSGGDILPQLFPRFFRDGFSGGAWTSTFQDQVISLTPRASLASLGSPLTGEAYQAVNNVFGSISSKAGTVESSILSKSTADNDRTSAIKGSPKAKNQKSLKFPLNEDTLALLKKRIDYFSTPTYASYGTLIDQPRTNKRNLEISANCHRELSHSVVIIEMLENLDLRFFLNMRNLAYNHALDEDSEELRQHTMTSSATLLMEFFDIKQALYDVTIKKVMDIQNLTLDDPFVFCSMAGDLSFGGEREEGSREEHVLKQERLAEAYRKRLVAEDVEVNDMTYFNPDTEVKATQISFLAVIDSTYQVVEQLIQARENVLNYAARMMKNDLISELTRGEQEKIFEDDKIDAKTSDAEIEHIGPKDFGDSSFTSDVPWYLDSEHEYSLIYDNQGRIKGGTKVALLEHLTSHRAIDASFNIAMLLSFRSIFTTSEFLHALVERYHLYPPEGLSFEEYSAWIEKKSTPVKTRVVNILKTLFSNYWTPAYYEPGIDDLVSFAQLATAQSISGAPALLVELKNRLSLKGNLKNFVPETIRFDESGHNYSGNALNPRVSTGGPAEFGAGYGFRMRKLKLLDIDPQTFAKQLTTKEHYLYSKITPFECLDRIWSKKYCYFGGSEDISKFISSANALTSYVSFAIVKQTNTKKRAKVLQHFISVAEYCYELNNFSSMTAIVSALYSSPIFRLKKSWSAVPEDSKKVLENLNTLMDPAKNFITYRNWLKTVHDVACVPFFGVYLSDLTFIAEGNPNCLHRSPEIINFSKRLRIVDILKEISSYQIIRYKFKRYDDIQSFIEESMKNIPNIEKQYEQSLRLEPRTEVSAGLSSGGTGMSKTDLGKKLEKKSRFMKNKKRPTKLVSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5E322</accession>
    <name>C5E322_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0H09658p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0H09658g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IML2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556295.1">
        <property type="nucleotide sequence ID" value="XM_002556249.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E322"/>
    <dbReference type="FunCoup" id="C5E322">
        <property type="interactions" value="152"/>
    </dbReference>
    <dbReference type="GeneID" id="8294620"/>
    <dbReference type="KEGG" id="lth:KLTH0H09658g"/>
    <dbReference type="eggNOG" id="KOG3783">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014926_0_0_1"/>
    <dbReference type="InParanoid" id="C5E322"/>
    <dbReference type="OMA" id="WNGYNRM"/>
    <dbReference type="OrthoDB" id="2154985at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019412">
        <property type="entry name" value="IML2/TPR_39"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31859">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 39 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31859:SF1">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 39C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10300">
        <property type="entry name" value="Iml2-TPR_39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR30433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="725" mass="81781" checksum="CC5B8DF90AC48391" modified="2009-07-28" version="1">MFRVLGALTGTGGPAKLDQEEQSEKILSQAHDFEVALQAMDYVLDDHPGKGLQLLEGSGAKPKNEEDQCVRVLARGVIEFLEATLGFEASVMKKAAATLSEAQQLSAKRRQQFQKMGLKTSSLYPPGTEYAVTFAEACLLHALLMLFSESMVESAKALFKLRKAYHMLREILNDINAAKKKKGSAMYAQGNNESFASFVSADSSTFKSVDIPYSLSTEEQQNKELMELAENVYNMRKKRLEGAHIGNSPAIERLRTDLGIQALKDLPKKKEAKKPVTLSKAEIDKDQATIDEFIHSGVNLCFGILQVVLSLIPPAIGAVLSVVGFHGSREEGLRLVWRSTKDRNIHGGIGLLGLLFYYDGPFQFTDVDFDVPATSDGLKKTSSNDLDNSTLLHPGKMLEDALLQARALFPNSALWLLQEARMLSSRGRLQEAVDLMDSIDVSGIEMKQVRALMIFDRAMTLVHLHEFERAAEDLLSLLDINEWSHALYTYFAGCCYLENYRMCQMGLMEYSRIDFFKERATTLIFDAPNMLSRKKFMSKNLPLDRFMLRKVEQFKKFQSQLQLQDPLDAIGVSPVHELTYFYNGYNRMTEDCLKLSHKLLTEYHNPTIDAKEPNQELVKNLLTSLTSRRLGKVQEGCDMLDKNVLPQIITLQPDGKVKYIKKTEDPWAYPTALYERALFTWKLKGLDGLEECKMWLTRAQDYADDYELSARIGMKIKAAIDRLEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>C5E357</accession>
    <name>C5E357_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0H10560p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0H10560g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556330.1">
        <property type="nucleotide sequence ID" value="XM_002556284.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E357"/>
    <dbReference type="FunCoup" id="C5E357">
        <property type="interactions" value="149"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E357"/>
    <dbReference type="GeneID" id="8294661"/>
    <dbReference type="KEGG" id="lth:KLTH0H10560g"/>
    <dbReference type="eggNOG" id="KOG2793">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049351_1_0_1"/>
    <dbReference type="InParanoid" id="C5E357"/>
    <dbReference type="OMA" id="DDFGEMK"/>
    <dbReference type="OrthoDB" id="433955at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614:SF156">
        <property type="entry name" value="PROTEIN-LYSINE N-METHYLTRANSFERASE EFM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10294">
        <property type="entry name" value="Methyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR30468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="417" mass="46523" checksum="5997D79703DF9758" modified="2009-07-28" version="1">MFDPLDFLSGSEKPSACAGNQMRSISSDTLVTVIQPQESAYNDRDLEEEVDAPIGVIDLPHVAIAPPSVVLTALLLLRPSINVNFSERDDTIDRSVESVCREKDITVSQIEAFASWCSGFGMSVLDTPAKICARVPGLASTADGKDLLMYYTMVLSKYEKMQTADQVNERILKEASMRISEKCGRTAQPAMNRIFKIGGLRTGVKLHEPALTSDNLGLKTWGASLVLARKLCENFSKFERQRDLRILELGAGTGLVGISLVLKMLESNSGHNCSMHLTDLPEIVTNLKENVKINCCNSRSDLKVYADVLDWTNPDSFEKTYGAHKFDVLLIADPVYSPQHPQWIVDMISKFLSPRGVLYLEVPIRQKYHDERQHLWGLIRDASLQVIKEELDEGVDDWGKVSYLYKKIVPVNANQSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5E380</accession>
    <name>C5E380_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0H11066p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0H11066g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SecY/SEC61-alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556353.1">
        <property type="nucleotide sequence ID" value="XM_002556307.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E380"/>
    <dbReference type="FunCoup" id="C5E380">
        <property type="interactions" value="200"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E380"/>
    <dbReference type="GeneID" id="8294684"/>
    <dbReference type="KEGG" id="lth:KLTH0H11066g"/>
    <dbReference type="eggNOG" id="KOG1373">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031763_2_1_1"/>
    <dbReference type="InParanoid" id="C5E380"/>
    <dbReference type="OMA" id="QAYCHIK"/>
    <dbReference type="OrthoDB" id="420669at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3370.10">
        <property type="entry name" value="SecY subunit domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002208">
        <property type="entry name" value="SecY/SEC61-alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023201">
        <property type="entry name" value="SecY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019561">
        <property type="entry name" value="Translocon_Sec61/SecY_plug_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10906">
        <property type="entry name" value="SECY/SEC61-ALPHA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10559">
        <property type="entry name" value="Plug_translocon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00344">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004557">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103491">
        <property type="entry name" value="Preprotein translocase SecY subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="81"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="121"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="246"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="287"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="364"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="421"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="449"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="domain" description="Translocon Sec61/SecY plug" evidence="3">
        <location>
            <begin position="41"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU004349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR30491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="490" mass="53107" checksum="9E7571B245ED25A9" modified="2009-07-28" version="1">MAGFRLIELAKPFLPLLPEVEVPYEKIGFDDKVVYTIIAALIYLFGQFPLAGVSKEASGVLDPLYFLRSVFAAEPKTLLEFGVFPVVSSALIMQVLAGLKLIKVNFKIRQDRELFQTCTKLFALLQYLVLANVFIFSGYYGTDLSVVQITALNVQLVVAGFFVTLLVEVIDKGYGFASGAMAITTVTISTNFVADVFGVGQFPVDNEGHTEPQGAFINLIQSLRASHKTWTGAIVGAFNRDYLPNLTTAFLVLALAASICFLTNFRLELPIRSTRTRGVNNVYPIRLFYVGSLSVLFAYVILFYIHVGAFVLVQLIAHNNPSSIIYKVVGGYASHNNLLYVPQFPLSLLTPPKSFVECITRQPLTPLFFTTFLVITGIWFAGLWQQISGSSARDISEQFKEQGITLTGRREQGVTKELDKIVPVAATTGAIVLGLLVACGELLGLKGKGASIIVGVSGGFALLELITAEYQQSGGQSALAQVLGAPSGGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3F6</accession>
    <name>C5E3F6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">asparagine--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Asparaginyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0H13024g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>tRNA(Asn) + L-asparagine + ATP = L-asparaginyl-tRNA(Asn) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11180"/>
            <dbReference type="Rhea" id="RHEA-COMP:9659"/>
            <dbReference type="Rhea" id="RHEA-COMP:9674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78515"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.22"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.22" evidence="3"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556429.1">
        <property type="nucleotide sequence ID" value="XM_002556383.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3F6"/>
    <dbReference type="FunCoup" id="C5E3F6">
        <property type="interactions" value="970"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3F6"/>
    <dbReference type="GeneID" id="8294772"/>
    <dbReference type="KEGG" id="lth:KLTH0H13024g"/>
    <dbReference type="eggNOG" id="KOG0555">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004553_2_10_1"/>
    <dbReference type="InParanoid" id="C5E3F6"/>
    <dbReference type="OMA" id="DCCLYPR"/>
    <dbReference type="OrthoDB" id="1931232at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004816">
        <property type="term" value="F:asparagine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006421">
        <property type="term" value="P:asparaginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04323">
        <property type="entry name" value="AsnRS_cyto_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00776">
        <property type="entry name" value="AsxRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000040">
        <property type="entry name" value="Asparagine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1910.20">
        <property type="entry name" value="asparaginyl-tRNA synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004522">
        <property type="entry name" value="Asn-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048952">
        <property type="entry name" value="AsnRS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00457">
        <property type="entry name" value="asnS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594:SF16">
        <property type="entry name" value="ASPARAGINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594">
        <property type="entry name" value="ASPARTYL/LYSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20917">
        <property type="entry name" value="AsnRS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="14">
        <location>
            <begin position="251"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="74"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR30567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="554" mass="62245" checksum="E8883833691EEA8D" modified="2009-07-28" version="1">MSLYINESTGVDDISVTGSQDQPFKTPAYALFSAREDDEEPKLFVFKKDEEKGDCFVEISASALKKARKGSDGLKKKLAKQLELEKKKQEKDENEAAKKLEALNIKIEEDKSLPEAIKCKIQKSYDAVDQRVKVSGWIHRLRSSKSLVFIVLRDGTGFLQAVLTGDLARAYQTTALTLESTVTLYGKVCKLPEGKNAPGGVELLVDYYEVVSLAPSGEDAFTNKIAEGADPSLLLDQRHLALRGETLSGVMKVRAALLRAVRRFYYEEGLTEVNTPCMVQTQVEGGSTLFNLGYYGEEAYLTQSSQLYLETCLASLGDVYCIQESFRAEKSHTRRHLSEYTHIEAELCFLTFDDLLVHIEKLITTAVRYVLEDPVAGPLVEQLNPGFKAPTMPFMRLDYRDAIKWLNEHGIKNEEGEDFKFGDDIAEAAERKMTDTIGVPIFLIRFPVEIKSFYMKRCADDANVTESVDVLMPNVGEITGGSMRIDAMDELMAGFKREGIATDPYYWFIDQRKYGSCPHGGYGIGTERILAWLCDRFTVRDCSLYPRFTGRCKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3N0</accession>
    <name>C5E3N0_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DASH complex subunit DAD4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Outer kinetochore protein DAD4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">KLTH0H14916g</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
            <location evidence="3">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DASH complex DAD4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556503.1">
        <property type="nucleotide sequence ID" value="XM_002556457.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3N0"/>
    <dbReference type="FunCoup" id="C5E3N0">
        <property type="interactions" value="29"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3N0"/>
    <dbReference type="GeneID" id="8294867"/>
    <dbReference type="KEGG" id="lth:KLTH0H14916g"/>
    <dbReference type="eggNOG" id="ENOG502S890">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_177920_0_0_1"/>
    <dbReference type="InParanoid" id="C5E3N0"/>
    <dbReference type="OMA" id="SQMWANY"/>
    <dbReference type="OrthoDB" id="5516652at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042729">
        <property type="term" value="C:DASH complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="C:mitotic spindle"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008608">
        <property type="term" value="P:attachment of spindle microtubules to kinetochore"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013959">
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    </dbReference>
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        <property type="entry name" value="DASH COMPLEX SUBUNIT DAD4"/>
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        <property type="entry name" value="DASH COMPLEX SUBUNIT DAD4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DASH_Dad4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="15" id="KW-0137">Centromere</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="9" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="13"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00030569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAR30641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="72" mass="8364" checksum="9C741C957F4A614F" modified="2009-07-28" version="1">MENPYEQVHQNILSRIINNVERLNESVVTLNQELQHINARNSNLEIMGQMCENYHKSVQFNMSATGHKQSPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3P2</accession>
    <name>C5E3P2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0H15202p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0H15202g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556515.1">
        <property type="nucleotide sequence ID" value="XM_002556469.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3P2"/>
    <dbReference type="FunCoup" id="C5E3P2">
        <property type="interactions" value="256"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3P2"/>
    <dbReference type="GeneID" id="8294880"/>
    <dbReference type="KEGG" id="lth:KLTH0H15202g"/>
    <dbReference type="eggNOG" id="KOG2330">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014435_2_1_1"/>
    <dbReference type="InParanoid" id="C5E3P2"/>
    <dbReference type="OMA" id="IEWFDCD"/>
    <dbReference type="OrthoDB" id="10260794at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007180">
        <property type="entry name" value="DUF382"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006568">
        <property type="entry name" value="PSP_pro-rich"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052584">
        <property type="entry name" value="U2_snRNP_Complex_Component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12785">
        <property type="entry name" value="SPLICING FACTOR 3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12785:SF6">
        <property type="entry name" value="SPLICING FACTOR 3B SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04037">
        <property type="entry name" value="DUF382"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04046">
        <property type="entry name" value="PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00581">
        <property type="entry name" value="PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PSP proline-rich" evidence="2">
        <location>
            <begin position="280"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="421"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="214"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="461"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="485"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR30653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="495" mass="56364" checksum="CF3E9418BE6B7F33" modified="2009-07-28" version="1">MGKSVKSRRRQKAKKSSCLEKSSAARRELASLIDNVRLERNPEKHDMQITSPSTFHEDVVPDQFKAVFAKFQVQSEPEKPDSLSSRSRDSDAVADSRNNEMFPDSKSAITTDEKTKVSKKKLRKLSKPALSELKSVVVYPEVIQWYDCDAPDPVLLSKIKSQKNVVQVPGHWQLKREYLSGRSVVAKRPFELPDVIRQTNIEEMRSTLPAQDEPNEKTLKESSRARIRPKLGSLDLDYRKLHDAFFKLGAHWKPDLMLPYGDLFYENRNLEEETKWSLMEKEKRPGTIGKDLRTALGLPEGKLPPWCYKFKELGMPPSYPGYKVAGINWDISNLRGDVYGTLGTKLKTQRESELFGKMVKADDSEGGSENEVGDAVDEEKDIAHLRNEDADTLSDAKEAQRQQELEAKIKAESMKEVLKRRQAHAKQQNSDAPKRLYSIIEQKEGSNGPGSVEGNVLYDIKGSRYGKEESPSENENKKQRTIQTNERDVTETFKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3P4</accession>
    <name>C5E3P4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0H15246p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0H15246g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556517.1">
        <property type="nucleotide sequence ID" value="XM_002556471.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3P4"/>
    <dbReference type="FunCoup" id="C5E3P4">
        <property type="interactions" value="212"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3P4"/>
    <dbReference type="GeneID" id="8294882"/>
    <dbReference type="KEGG" id="lth:KLTH0H15246g"/>
    <dbReference type="eggNOG" id="KOG0756">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052717_0_0_1"/>
    <dbReference type="InParanoid" id="C5E3P4"/>
    <dbReference type="OMA" id="VMGPCTY"/>
    <dbReference type="OrthoDB" id="10253709at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005371">
        <property type="term" value="F:tricarboxylate secondary active transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015742">
        <property type="term" value="P:alpha-ketoglutarate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006843">
        <property type="term" value="P:mitochondrial citrate transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000053">
        <property type="entry name" value="Mitochondrial DNA replication protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000085">
        <property type="entry name" value="Tricarboxylate carrier, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053017">
        <property type="entry name" value="Mito_Cit/Oxoglu_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46982">
        <property type="entry name" value="CITRATE/OXOGLUTARATE CARRIER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46982:SF1">
        <property type="entry name" value="CITRATE_OXOGLUTARATE CARRIER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="5">
        <location>
            <begin position="210"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR30655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="311" mass="33503" checksum="7B2D46258B7A4A2D" modified="2009-07-28" version="1">MSIAPTQPAELKKKPVSFSNILLGAGLNLCEVTTLGQPLEVTKTTMAANRNLNFLQSVKHIWSRGGVFGFYQGLIPWAWIEASTKGAVLLFVSAEAEYQFKSLGLGNFGAGIMGGVSGGVAQAYLTMGFCTCMKTVEITKAKSVAGGAPQSSWAAFKDIYRKEGIRGINKGVNAVAIRQMTNWGSRFGLSRLVEEGIRNVTGKTNPDEKLSPLEKIFASAVGGGLSAWNQPIEVIRVEMQSKKEDPNRPKNLTVGSAFRYIYQSSGLKGLYRGVTPRIGLGVWQTVFMVGFGDIAKEFVGKLTGETPVAKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-02-15" modified="2025-06-18" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q5K460</accession>
    <name>Q5K460_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">ATP synthase subunit 9, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">atp9</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <geneLocation type="mitochondrion" evidence="16"/>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2005" name="FEBS Lett." volume="579" first="30" last="40">
            <title>The complete mitochondrial genome of the yeast Kluyveromyces thermotolerans.</title>
            <authorList>
                <person name="Talla E."/>
                <person name="Anthouard V."/>
                <person name="Bouchier C."/>
                <person name="Frangeul L."/>
                <person name="Dujon B."/>
            </authorList>
            <dbReference type="PubMed" id="15620687"/>
            <dbReference type="DOI" id="10.1016/j.febslet.2004.10.106"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. A homomeric c-ring of probably 10 subunits is part of the complex rotary element.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Mitochondrion membrane</location>
            <topology evidence="2 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the ATPase C chain family.</text>
    </comment>
    <dbReference type="EMBL" id="AJ634268">
        <property type="protein sequence ID" value="CAG25604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_184726.1">
        <property type="nucleotide sequence ID" value="NC_006626.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q5K460"/>
    <dbReference type="FunCoup" id="Q5K460">
        <property type="interactions" value="925"/>
    </dbReference>
    <dbReference type="STRING" id="559295.Q5K460"/>
    <dbReference type="GeneID" id="3239002"/>
    <dbReference type="InParanoid" id="Q5K460"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Mitochondrion"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033177">
        <property type="term" value="C:proton-transporting two-sector ATPase complex, proton-transporting domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015078">
        <property type="term" value="F:proton transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015986">
        <property type="term" value="P:proton motive force-driven ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18182">
        <property type="entry name" value="ATP-synt_Fo_c_ATP5G3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.20.10:FF:000014">
        <property type="entry name" value="ATP synthase subunit 9, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.20.10">
        <property type="entry name" value="F1F0 ATP synthase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01396">
        <property type="entry name" value="ATP_synth_c_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000454">
        <property type="entry name" value="ATP_synth_F0_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020537">
        <property type="entry name" value="ATP_synth_F0_csu_DDCD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038662">
        <property type="entry name" value="ATP_synth_F0_csu_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002379">
        <property type="entry name" value="ATPase_proteolipid_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035921">
        <property type="entry name" value="F/V-ATP_Csub_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10031">
        <property type="entry name" value="ATP SYNTHASE LIPID-BINDING PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10031:SF0">
        <property type="entry name" value="ATPASE PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00137">
        <property type="entry name" value="ATP-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00124">
        <property type="entry name" value="ATPASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81333">
        <property type="entry name" value="F1F0 ATP synthase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00605">
        <property type="entry name" value="ATPASE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0138">CF(0)</keyword>
    <keyword evidence="8 14" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="10 14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 14" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="13 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 14 16" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 14" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="46"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="15">
        <location>
            <begin position="10"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAG25604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="76" mass="7819" checksum="9A10592DA46E76E2" modified="2005-02-15" version="1">MQLVLAAKYIGAGISTIGLLGAGIGIAIVFAALINGVSRNPSLKDTLFPFAILGFALSEATGLFCLMISFLLLYGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>C5DB99</accession>
    <name>C5DB99_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Protein transport protein SEC23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ordered locus">KLTH0A00814g</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 14">Cytoplasmic vesicle</location>
            <location evidence="1 14">COPII-coated vesicle membrane</location>
            <topology evidence="1 14">Peripheral membrane protein</topology>
            <orientation evidence="1 14">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 14">Endoplasmic reticulum membrane</location>
            <topology evidence="2 14">Peripheral membrane protein</topology>
            <orientation evidence="2 14">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Golgi apparatus membrane</location>
            <topology evidence="14">Peripheral membrane protein</topology>
            <orientation evidence="14">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the SEC23/SEC24 family. SEC23 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551498.1">
        <property type="nucleotide sequence ID" value="XM_002551452.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DB99"/>
    <dbReference type="FunCoup" id="C5DB99">
        <property type="interactions" value="1110"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DB99"/>
    <dbReference type="GeneID" id="8290289"/>
    <dbReference type="KEGG" id="lth:KLTH0A00814g"/>
    <dbReference type="eggNOG" id="KOG1986">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008658_3_0_1"/>
    <dbReference type="InParanoid" id="C5DB99"/>
    <dbReference type="OMA" id="FPPHYAE"/>
    <dbReference type="OrthoDB" id="10256289at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11287">
        <property type="entry name" value="Sec23_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.730:FF:000001">
        <property type="entry name" value="Protein transport protein SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.380:FF:000001">
        <property type="entry name" value="Protein transport protein SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000006">
        <property type="entry name" value="Protein transport protein SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000008">
        <property type="entry name" value="Protein transport protein SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1670">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.730">
        <property type="entry name" value="Sec23/Sec24 helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012990">
        <property type="entry name" value="Beta-sandwich_Sec23_24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007123">
        <property type="entry name" value="Gelsolin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036180">
        <property type="entry name" value="Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037364">
        <property type="entry name" value="Sec23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006900">
        <property type="entry name" value="Sec23/24_helical_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036175">
        <property type="entry name" value="Sec23/24_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006896">
        <property type="entry name" value="Sec23/24_trunk_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037550">
        <property type="entry name" value="Sec23_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006895">
        <property type="entry name" value="Znf_Sec23_Sec24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036174">
        <property type="entry name" value="Znf_Sec23_Sec24_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11141">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11141:SF0">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00626">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08033">
        <property type="entry name" value="Sec23_BS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04815">
        <property type="entry name" value="Sec23_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04811">
        <property type="entry name" value="Sec23_trunk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04810">
        <property type="entry name" value="zf-Sec23_Sec24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82754">
        <property type="entry name" value="C-terminal, gelsolin-like domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81811">
        <property type="entry name" value="Helical domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82919">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13 14" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7 14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 14" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="11 14" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 14" id="KW-0653">Protein transport</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 14" id="KW-0813">Transport</keyword>
    <keyword evidence="8 14" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zinc finger Sec23/Sec24-type" evidence="16">
        <location>
            <begin position="53"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 trunk" evidence="17">
        <location>
            <begin position="120"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 beta-sandwich" evidence="19">
        <location>
            <begin position="401"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 helical" evidence="18">
        <location>
            <begin position="522"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="15">
        <location>
            <begin position="634"/>
            <end position="722"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU365030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF04810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF04815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF08033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CAR21056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="766" mass="84608" checksum="327D8E4E7BC8F068" modified="2009-07-28" version="1">MDFETNEDINGIRFSWNVFPSSRTDANKNVVPVGCLYTPLKSKEDLMVMSYNPVVCGGPQCKAVLNPYCEIDLRSNAWACPICNSRNHLPAHYANMSQENMPAELSNTTIEYITNRPVQVPPVFLFVVDITAEEENLQALKESIIASLSLLPPNALVGLITYGNVVHLHDLSCDTIDKCNVFRGDREYQLLQLIEMLTGEKSGVAGAAGGAVGANAALNKITPFSLNRFFLPLEQVEFKLTQLLENLSPDQWSIPAGHRPLRATGSALNIATLVLQGCYKNVASRIILFASGPGTLAPGLIVSSELKDPLRSHHDIDSDNAKHYKKASKFYSMLADRIAQNGHTVDIFAGCYDQVGMSEMKYLTDTTGGVLLLTDAFSTSIFKQSFLRLFSSDDEGYLTMAFNGNMSVKTSRDLKVQGLIGHASPVKKTDATNISDSEIGVGGTSTWKMSSITPNHSYAVFFEIANNAAASAGVVGGDRPKLAYTQFVTSYQHASGTNRVRVTTVANQLLPFGSPAIAASFDQEAAAVLMARIAVHKAESDDGADVIRWIDRTLIKLCQKYADYNKDDPRSFRLAPNFSLYPQFTYYLRRSQFLSVFNNSPDETAFYRHIFTREDTTNSLIMIQPTLTAFFMEEEPQPVLLDSVSVKPNTILLLDTFFYILIYHGEQIAQWRKAGYQDDPQYADFKSLLEEPKLEAAELLVDRFPLPRFIDTEAGGSQARFLLSKLNPSDSYQDQSAGGSTIVLTDDVSLQNFMLHLQEVTVSGQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBE3</accession>
    <name>C5DBE3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ADP-ribose 1''-phosphate phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.84</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0A01892g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>ADP-alpha-D-ribose 1''-phosphate + H2O = ADP-D-ribose + phosphate</text>
            <dbReference type="Rhea" id="RHEA:25029"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57967"/>
            <dbReference type="ChEBI" id="CHEBI:58753"/>
            <dbReference type="EC" id="3.1.3.84"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the POA1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.84" evidence="2"/>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551542.1">
        <property type="nucleotide sequence ID" value="XM_002551496.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBE3"/>
    <dbReference type="FunCoup" id="C5DBE3">
        <property type="interactions" value="9"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBE3"/>
    <dbReference type="GeneID" id="8290337"/>
    <dbReference type="KEGG" id="lth:KLTH0A01892g"/>
    <dbReference type="eggNOG" id="ENOG502S60W">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054419_1_2_1"/>
    <dbReference type="InParanoid" id="C5DBE3"/>
    <dbReference type="OMA" id="HATNCIA"/>
    <dbReference type="OrthoDB" id="2155246at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140291">
        <property type="term" value="P:peptidyl-glutamate ADP-deribosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02901">
        <property type="entry name" value="Macro_Poa1p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="entry name" value="Leucine Aminopeptidase, subunit E, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050892">
        <property type="entry name" value="ADP-ribose_metab_enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002589">
        <property type="entry name" value="Macro_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12521:SF0">
        <property type="entry name" value="ADP-RIBOSE GLYCOHYDROLASE OARD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12521">
        <property type="entry name" value="PROTEIN C6ORF130"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01661">
        <property type="entry name" value="Macro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00506">
        <property type="entry name" value="A1pp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="Macro domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51154">
        <property type="entry name" value="MACRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Macro" evidence="5">
        <location>
            <begin position="1"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR21100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="183" mass="19987" checksum="2F464FE02FA38936" modified="2009-07-28" version="1">MGVSNVRYVRGNCLAASISHPRILIHSCNCNGSWGGGIAYQLGAKFPKAEGDYIELCEKFGSSLLGKFVLIPSYTDSTLLIGCLFTASFGGSQQGTGDSILSYTKQALEDMRIKIFSDTGSGGEIDSFSRKYIQRFKDKLKVPVADYKLEMPQINSGIFGVPWPKTEQLLAEQSSLSFTVYVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBL2</accession>
    <name>C5DBL2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">F-actin-capping protein subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0A03520g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9">F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Heterodimer of an alpha and a beta subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
            <location evidence="1 9">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the F-actin-capping protein beta subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551611.1">
        <property type="nucleotide sequence ID" value="XM_002551565.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBL2"/>
    <dbReference type="FunCoup" id="C5DBL2">
        <property type="interactions" value="937"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBL2"/>
    <dbReference type="GeneID" id="8290411"/>
    <dbReference type="KEGG" id="lth:KLTH0A03520g"/>
    <dbReference type="eggNOG" id="KOG3174">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045864_1_1_1"/>
    <dbReference type="InParanoid" id="C5DBL2"/>
    <dbReference type="OMA" id="WSNKYYP"/>
    <dbReference type="OrthoDB" id="9979678at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008290">
        <property type="term" value="C:F-actin capping protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051016">
        <property type="term" value="P:barbed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.570:FF:000001">
        <property type="entry name" value="F-actin-capping protein subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.210">
        <property type="entry name" value="F-actin capping protein, beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037282">
        <property type="entry name" value="CapZ_alpha/beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042276">
        <property type="entry name" value="CapZ_alpha/beta_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001698">
        <property type="entry name" value="CAPZB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043175">
        <property type="entry name" value="CAPZB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019771">
        <property type="entry name" value="F-actin_capping_bsu_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10619">
        <property type="entry name" value="F-ACTIN-CAPPING PROTEIN SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10619:SF0">
        <property type="entry name" value="F-ACTIN-CAPPING PROTEIN SUBUNIT BETA ISOFORMS 1 AND 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01115">
        <property type="entry name" value="F_actin_cap_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00192">
        <property type="entry name" value="FACTINCAPB"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90096">
        <property type="entry name" value="Subunits of heterodimeric actin filament capping protein Capz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00231">
        <property type="entry name" value="F_ACTIN_CAPPING_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0117">Actin capping</keyword>
    <keyword evidence="6 9" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="258"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR21169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="281" mass="31641" checksum="C5CFBC0B0A62566F" modified="2009-07-28" version="1">MSPYEAALDLLRRLDPTKVEQNLNQLIKLDPDLAEDLLASVDTPLKVKKDPNASQKEFLCCDYNRDIDSHRSPWSNNYVPALSAQELEESPFPSDAIRAVEVLANDSFDIYRDLYYEGGVSSVYLWDLGDDADADGAPNDFAGVVLLKKGENSRSSWDAIHVFEVLQEGSTQSQVSYRITTTIILHLNSEKKMTLAGNLTRQTERSFALPGHLSPEQSGIWHVSNLGSVIEEVESQMRNQLEVVYFEKTKDIYRELRDSSQEATTQNRSVHEEVVKGLQGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBQ1</accession>
    <name>C5DBQ1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0A04444p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0A04444g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551650.1">
        <property type="nucleotide sequence ID" value="XM_002551604.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBQ1"/>
    <dbReference type="FunCoup" id="C5DBQ1">
        <property type="interactions" value="192"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBQ1"/>
    <dbReference type="GeneID" id="8290453"/>
    <dbReference type="KEGG" id="lth:KLTH0A04444g"/>
    <dbReference type="eggNOG" id="ENOG502QVE1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006698_0_0_1"/>
    <dbReference type="InParanoid" id="C5DBQ1"/>
    <dbReference type="OMA" id="FVMERFF"/>
    <dbReference type="OrthoDB" id="4089008at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007147">
        <property type="entry name" value="TF_Vhr"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04001">
        <property type="entry name" value="Vhr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="286"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="571"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="132"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="148"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="167"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="590"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="616"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="650"/>
            <end position="662"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="673" mass="74207" checksum="4DABB8CFA38A4BFA" modified="2009-07-28" version="1">MIKPPRVQKPSSSGTTHKIRKQLNFTDERRWKQFSSRRLELIEKFGLGERKASEQDKSIRQIATILRTEFGYPLSSSSEFEKLVTAAVQSVRRNRKRSSKSKPSNPQAQESVLEPFPVMTTTRESSGTDNLFFDRDGDQFARADRYTSGSNSSSSPPESSLTSFISHNQTLPTLFSHSGQRSGPTKNASTLGAVPHALPHLQPKPLSSSNKPVGDCENPGSSIVTAHTQNYDEVIKAVIADMVQNRVALHVQSRNDEVTPNLADFALSSNDGNLLNIAFQSHVAPSNPHTTAYPKARNPSGNDSNESKIPFFLREKILLHVQRSRACLEVSTAQGSLELYTNLSILGETSVKASVAFVMERFFSNLSSSSMEYVTSKLCSDQELALISVRIFGPATERHFDQLPHGAQLKLFYIVIGGIIKDFGFDPCIYPLSEIMHDVILRQFPLGHHPKGDFKRAAVMSTVSMAPAAANKDVYRVVLLKFKDKEQRFTFHLLSNGPPTIAEIIANSRSLFHIVSQTKSLYLIHNNRVINDDMELARVFNRFTSEEIVIELRDVKPEPLDGLAMLSTVSASEFSDPGSQKALPNRQNGASTTPTAYYPAASPLGPDCHSKESGDSENMPSTSTIQSDRDKVASEGSGAKEQAENEQKTGSEVGQNPETSASAPPRRRFQPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBS7</accession>
    <name>C5DBS7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">2-oxoglutarate reductase</fullName>
            <ecNumber evidence="4">1.1.1.399</ecNumber>
            <ecNumber evidence="5">1.1.1.95</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">KLTH0A05038g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible oxidation of 3-phospho-D-glycerate to 3-phosphonooxypyruvate, the first step of the phosphorylated L-serine biosynthesis pathway. Also catalyzes the reversible oxidation of 2-hydroxyglutarate to 2-oxoglutarate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>(2R)-3-phosphoglycerate + NAD(+) = 3-phosphooxypyruvate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12641"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18110"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="EC" id="1.1.1.95"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(R)-2-hydroxyglutarate + NAD(+) = 2-oxoglutarate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:49612"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15801"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.399"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.399" evidence="4"/>
    <dbReference type="EC" id="1.1.1.95" evidence="5"/>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551676.1">
        <property type="nucleotide sequence ID" value="XM_002551630.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBS7"/>
    <dbReference type="FunCoup" id="C5DBS7">
        <property type="interactions" value="718"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBS7"/>
    <dbReference type="GeneID" id="8290480"/>
    <dbReference type="KEGG" id="lth:KLTH0A05038g"/>
    <dbReference type="eggNOG" id="KOG0068">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019796_9_2_1"/>
    <dbReference type="InParanoid" id="C5DBS7"/>
    <dbReference type="OMA" id="SKGCWEV"/>
    <dbReference type="OrthoDB" id="1621027at2759"/>
    <dbReference type="UniPathway" id="UPA00135">
        <property type="reaction ID" value="UER00196"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061759">
        <property type="term" value="F:2-oxoglutarate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004617">
        <property type="term" value="F:phosphoglycerate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006564">
        <property type="term" value="P:L-serine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04901">
        <property type="entry name" value="ACT_3PGDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12176">
        <property type="entry name" value="PGDH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.260:FF:000036">
        <property type="entry name" value="D-3-phosphoglycerate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000041">
        <property type="entry name" value="D-3-phosphoglycerate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050857">
        <property type="entry name" value="D-2-hydroxyacid_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029752">
        <property type="entry name" value="D-isomer_DH_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008759">
        <property type="entry name" value="PRK11790.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42789">
        <property type="entry name" value="D-ISOMER SPECIFIC 2-HYDROXYACID DEHYDROGENASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_6G10090)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42789:SF1">
        <property type="entry name" value="D-ISOMER SPECIFIC 2-HYDROXYACID DEHYDROGENASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_6G10090)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00065">
        <property type="entry name" value="D_2_HYDROXYACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00671">
        <property type="entry name" value="D_2_HYDROXYACID_DH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="8 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0718">Serine biosynthesis</keyword>
    <feature type="domain" description="ACT" evidence="15">
        <location>
            <begin position="400"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAR21234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="470" mass="50886" checksum="F9D63E7D5035BFA6" modified="2009-07-28" version="1">MASPQQINNLQNSFQSAVNLGGSPGIVSTSPTQSFMSSVPRRVSASRQTKALKPFSTGDIKILLLENVNQTAVEIFNGQGYQVEFHKSSLSEDELIEKIRDVHAVGIRSKTRLTEKVLKHAKNLVVIGCFCIGTNQVDLDYAAKIGVAVFNSPFSNSRSVAELIMAEVISLARQLGDRSIEMHTGTWNKVASKCWEVRGKTLGIIGYGHIGSQLSVLAEAFGMQVLYYDIVTIMALGTAKQVSTLDKLLSESDFVTCHVPATEDTKNLLSAPQFAAMKDGSYVLNASRGTVIDIPSLIQAIKAGKIAGTALDVYPHEPAKNGAGAFNDELNSWISELVSLPNVILTPHIGGSTEEAQSAIGIEVATNLSKYINEGASVGSVNFPEVSLRGLDLDQENVVRVLYIHHNVPGVLKTVNNILSNHNIEKQFSDSSGDIAYLMADISSVNQSDIKDIYDQLNATEYKISIRLLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>C5DC83</accession>
    <name>C5DC83_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0B00924p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0B00924g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551832.1">
        <property type="nucleotide sequence ID" value="XM_002551786.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DC83"/>
    <dbReference type="FunCoup" id="C5DC83">
        <property type="interactions" value="1484"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DC83"/>
    <dbReference type="GeneID" id="8290658"/>
    <dbReference type="KEGG" id="lth:KLTH0B00924g"/>
    <dbReference type="eggNOG" id="KOG0301">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011791_2_0_1"/>
    <dbReference type="InParanoid" id="C5DC83"/>
    <dbReference type="OMA" id="STIMVKN"/>
    <dbReference type="OrthoDB" id="10265988at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010992">
        <property type="term" value="P:ubiquitin recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.870">
        <property type="entry name" value="PFU (PLAA family ubiquitin binding), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015155">
        <property type="entry name" value="PFU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038122">
        <property type="entry name" value="PFU_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013535">
        <property type="entry name" value="PUL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19849">
        <property type="entry name" value="PHOSPHOLIPASE A-2-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19849:SF0">
        <property type="entry name" value="PHOSPHOLIPASE A-2-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09070">
        <property type="entry name" value="PFU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08324">
        <property type="entry name" value="PUL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51394">
        <property type="entry name" value="PFU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51396">
        <property type="entry name" value="PUL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="12"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="101"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="139"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="180"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="221"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="PFU" evidence="7">
        <location>
            <begin position="355"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="PUL" evidence="8">
        <location>
            <begin position="467"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="445"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR21394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="714" mass="78738" checksum="6015EBEE53C65E85" modified="2009-07-28" version="1">MVKKVFKLSATLFGHEQDVKSVAVMSNDEVATCSRDGSVRLWKKGSNDMWQDTILYKVDEFINSLCYDKLSRLLYCGGKDTLINSISPLLGELGQEPEFVLVGHKSNVCALNSSQGYILSGSWDATAKVWFQGALKHELVGHKASVWDVKMLPEVGFYLTASADGTVKLWEGEKVLKTFSNLHTDVVRHLDISPDGDQFASCSNDGTIKINDMDGNTLRTLKGHESFVYCVKYTPCGGLVSCGEDRSVRVWGPDGAVKQVIRVPAVSVWDLDVLPNGDILVGCSDSLARIFTCHEGRVAPQQELDTFAKEVERTAISSQTMGFDESKLSPSEALLNPGKEGQVIVVKSPNGVNEAHQYTQGAWTKVGDVVSSAGNDKKKEFEGKMYDYVFDVDVTEGAPPLKLPFNANGNAYQAADDFLARYELPASYREEVVRFLITNTTGVSLEQDQSSTSNGQSNSIPQSKPMSVLPVREYLDIRSFSPDAIFNGVVKLNAQERSFDDEDLAAIGAALHDVEDNYELLFAQASIIRSSWSNKIPAYDIMRLIVEYLPHADAMSEFIEEGLGSAMPQLEMLTVRTLANCFKNKKWGTELMSKNAVYDSVFQTIEPNRPQASKTVNLAVSIATLIFNYSVMIIKSGNLDLLPTVADALNNKFGPSQMIQDSEEAAYRMLVAYGNLATVEPTLLQFAKSIAWIKPVKEKYGHLSRFSHVFADLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>C5DC98</accession>
    <name>C5DC98_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">phosphoserine phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">O-phosphoserine phosphohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0B01298g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HAD-like hydrolase superfamily. SerB family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.3" evidence="4"/>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551847.1">
        <property type="nucleotide sequence ID" value="XM_002551801.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DC98"/>
    <dbReference type="FunCoup" id="C5DC98">
        <property type="interactions" value="595"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DC98"/>
    <dbReference type="GeneID" id="8290674"/>
    <dbReference type="KEGG" id="lth:KLTH0B01298g"/>
    <dbReference type="eggNOG" id="KOG1615">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036368_4_0_1"/>
    <dbReference type="InParanoid" id="C5DC98"/>
    <dbReference type="OMA" id="LSMFKHA"/>
    <dbReference type="OrthoDB" id="27226at2759"/>
    <dbReference type="UniPathway" id="UPA00135">
        <property type="reaction ID" value="UER00198"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036424">
        <property type="term" value="F:L-phosphoserine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006564">
        <property type="term" value="P:L-serine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050582">
        <property type="entry name" value="HAD-like_SerB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004469">
        <property type="entry name" value="PSP"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01488">
        <property type="entry name" value="HAD-SF-IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00338">
        <property type="entry name" value="serB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43344">
        <property type="entry name" value="PHOSPHOSERINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43344:SF2">
        <property type="entry name" value="PHOSPHOSERINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01137">
        <property type="entry name" value="C1.6.1:_Phosphoserine_Phosphat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01136">
        <property type="entry name" value="C1.6:_Phosphoserine_Phosphatas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00029">
        <property type="entry name" value="phosphoserine_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0718">Serine biosynthesis</keyword>
    <feature type="active site" description="Nucleophile" evidence="11">
        <location>
            <position position="93"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="11">
        <location>
            <position position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR604469-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR21409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="311" mass="33875" checksum="BBDD8808B3EF6565" modified="2009-07-28" version="1">MSSAEIVVTLIAHGSELSEQTKSGFRAFLNDLSDVELEQEKALSVRAYDFFLRTQKDVAVIKETMGSLGNGILDVIVQKNSEWRKKKGLVVFDMDSTLIYQEVIELIAAYAQVEDRVAEITERAMNNELDFKQSLQERVALLKGIKTATLYDEIKAKLRVTEGVPELTRGLQKTGCKLAVLSGGFTPFANHMKEVLGLDFARANVLATETDASTGEEVLSGYTLGDVVDGECKAATLIELAREHGVPIEATVMVGDGGNDLPAMGVAGFGIAWNAKPKVQAAAPSKLNTRSMQDAFYIFGYTDGEIQELLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCG7</accession>
    <name>C5DCG7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Nucleolar complex-associated protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0B02926g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Required for synthesis of 60S ribosomal subunits and the transport of pre-ribosomes from the nucleoplasm to the cytoplasm.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
            <location evidence="1 5">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the CBF/MAK21 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551916.1">
        <property type="nucleotide sequence ID" value="XM_002551870.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCG7"/>
    <dbReference type="FunCoup" id="C5DCG7">
        <property type="interactions" value="1185"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCG7"/>
    <dbReference type="GeneID" id="8290748"/>
    <dbReference type="KEGG" id="lth:KLTH0B02926g"/>
    <dbReference type="eggNOG" id="KOG2153">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012441_3_1_1"/>
    <dbReference type="InParanoid" id="C5DCG7"/>
    <dbReference type="OMA" id="FGNMANF"/>
    <dbReference type="OrthoDB" id="10263597at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005612">
        <property type="entry name" value="CCAAT-binding_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011501">
        <property type="entry name" value="Noc3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016903">
        <property type="entry name" value="Nucleolar_cplx-assoc_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14428">
        <property type="entry name" value="NUCLEOLAR COMPLEX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14428:SF5">
        <property type="entry name" value="NUCLEOLAR COMPLEX PROTEIN 3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03914">
        <property type="entry name" value="CBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07540">
        <property type="entry name" value="NOC3p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028977">
        <property type="entry name" value="Nucleolar_complex_p3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Nucleolar complex-associated protein 3 N-terminal" evidence="9">
        <location>
            <begin position="132"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="CCAAT-binding factor" evidence="8">
        <location>
            <begin position="468"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="89"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="130"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="361"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="9"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="96"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR028977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR21478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="658" mass="74222" checksum="7E876815FECDADD1" modified="2009-07-28" version="1">MGSKKRSQRVAEDKASKRRKEQDSLLEGGIFTREDVQEIEDSIPAKKSWDDEEQDYERKPRKLAGYPEDNIEQLPIKIGAKVERRIVRAPKVEPESNNDDSEDVDLMKNDDEEQSTEADDSAVADSPEAVLQLKEQIAELVENIMEDAEENVGSLTRLRKMAQSKNSNTCKFSLLALIPVFKSVIPSYKIRPLSDAEKKEKVSKEVAKLRNFEEHLVRNYKEYIDLLSKLAKTPNSADQKQVAMGNLAANALVELLPSASNFNFSTEVFTAIIRRICKPNPLADPVYQKMVKALETLLNEDDDGRNSLEIVRILAKTIKSRSYMIDESVLNILLSLDVLQDFDPNTKDEDFTKVKVKKKDRAHLSKKQRKVRKEMKKVEEEMQKAEQTVLAEEREKNQAEILKFTLSLYLNILKSNVAKLIGSVLEGLSKFGHMANFDLLGDFLEVMRELIANSELDNLSPLEVRKVLLCIVTAFSLVSRHSQMKLSMDLTSFVDALYSVIPNLSVDAEIEYSHKTLRLADPLGSDLVKPSVNVSTKAELLLRALDHVFFRSRSGSKVRAAAFAKRLYVAMLATPEKTSIAILKFLEKLMDKYPEVGGLYSTEDRIGNGNFKLQAETPARSNAEAATIWENILLLKHYSPAVIKGTKALLEKSRGSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCN4</accession>
    <name>C5DCN4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">KLTH0B04510p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0B04510g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551983.1">
        <property type="nucleotide sequence ID" value="XM_002551937.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCN4"/>
    <dbReference type="FunCoup" id="C5DCN4">
        <property type="interactions" value="70"/>
    </dbReference>
    <dbReference type="GeneID" id="8290820"/>
    <dbReference type="KEGG" id="lth:KLTH0B04510g"/>
    <dbReference type="eggNOG" id="KOG0254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_30_3_1"/>
    <dbReference type="InParanoid" id="C5DCN4"/>
    <dbReference type="OMA" id="TQHFQRM"/>
    <dbReference type="OrthoDB" id="6133115at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015793">
        <property type="term" value="P:glycerol transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17356">
        <property type="entry name" value="MFS_HXT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000061">
        <property type="entry name" value="MFS sugar transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF55">
        <property type="entry name" value="SUGAR TRANSPORTER STL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="99"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="160"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="191"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="318"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="350"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="380"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="421"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="445"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="24"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="538"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="558"/>
            <end position="568"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR21545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="568" mass="62418" checksum="05B271FE3C351A96" modified="2009-07-28" version="1">MPSRLSVNRTSTLGLNGRSLRLAITITSVIGFSLFGYDQGLMSGLITGKEFNSEFPATGGDDRRTKLVQGAVTACYEIGCFFGSLFVMFRGEQIGRKPLVILGACLTIVGTVISTAAFGPHWGLGQFVVGRVCTGVGTGLNTSTIPVWQSEMSKPENRGILVNLEGSMIAVGTMIAYWIDFGLSYVDSSVQWRFPVAMQIFFALLLLAGIWELPDSPRWLMSRGRRDDALHVLAKLDNLPEDDDAIIAEATVIQDAVNRFRHEKRSVKDLFTGGKTQNLQRALVASSTQFFQQFTGCNAAIYYSTVLFQESIGLTGKLPLILGGVFATIYACFTIPSFFLVETLGRRKLFMLGAAGQAISFTITFGCLTKDDTEVAKGAAVGLFLFICFFGMSMLSLPWIYPPEIASMRVRSATNALSTCTNWLCNFAVVMFTPIFIMDTGYGCYLFFAVMNYLYLPVIFFFYPETAGRSLEEIDIIFAKAHVDGTMPWRVAANLPKLSFSEIEDQANALGLYEDDNEKQDLDLDEAADREDAIRQNSGVQGYTLFEKRENGAESGQPDGSSTSQKSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5DD33</accession>
    <name>C5DD33_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0B07920p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0B07920g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552132.1">
        <property type="nucleotide sequence ID" value="XM_002552086.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DD33"/>
    <dbReference type="FunCoup" id="C5DD33">
        <property type="interactions" value="16"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DD33"/>
    <dbReference type="GeneID" id="8290972"/>
    <dbReference type="KEGG" id="lth:KLTH0B07920g"/>
    <dbReference type="eggNOG" id="ENOG502QUF6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_454968_0_0_1"/>
    <dbReference type="InParanoid" id="C5DD33"/>
    <dbReference type="OMA" id="HNSAQNT"/>
    <dbReference type="OrthoDB" id="774557at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029005">
        <property type="entry name" value="LIM-bd/SEUSS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10378">
        <property type="entry name" value="LIM DOMAIN-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01803">
        <property type="entry name" value="LIM_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="367"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="468"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="71"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="367"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="478"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="525" mass="57570" checksum="0531F4AF54108677" modified="2009-07-28" version="1">MNSQQPHQGSQHMYGYQAPLRKQVPNFTPGVNMGQMMMGPQVNAHMGQQQPELGLHPTARQGHVQYSGAQISVPQPRKQQQQVQPQSQSNDLSNSTSAIPSPFATSMPTPTGPASAPPMNMHVGAPVSGAAQMPGATRSAGPVSATASTANHISGPGLTPASPQVGAMPSNQFYPVASAYQPVSKRHIASNMIPYPIRKYLSNMAILRLHEIINLVNVSTDRVDDFGYWQRFTNDLFTPYGILRYSTQGGEETRQFEFTTPIIPLICNSLGSVGIVRLEMIPQQLRAQVLSNGTIFFDCPRCTTTFYYPDGSYMTNFSQVKGIFDSNLKIEWVEISSYSFVPGIEWSSLERLISSEPVCHEIFKDLSNSRKKPESTEDPKNQEPARNQDPKVPDNFSAITKLRSQFGVFHNISSFGIQESFMRALQVSDAISYLKNLKVYQKVHNLQSPLTSLESFVASSQAEKASSMAPGAPFKYGNSSSPAATPSNPRPKVPNSSKPRPKKRRQSDLSPLSTGDRGLQGFGQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDB6</accession>
    <name>C5DDB6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">1,3-beta-glucan synthase</fullName>
            <ecNumber evidence="3">2.4.1.34</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">1,3-beta-D-glucan-UDP glucosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0B09856g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[(1-&gt;3)-beta-D-glucosyl](n) + UDP-alpha-D-glucose = [(1-&gt;3)-beta-D-glucosyl](n+1) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21476"/>
            <dbReference type="Rhea" id="RHEA-COMP:11146"/>
            <dbReference type="Rhea" id="RHEA-COMP:14303"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37671"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.4.1.34"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 48 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.34" evidence="3"/>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552215.1">
        <property type="nucleotide sequence ID" value="XM_002552169.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DDB6">
        <property type="interactions" value="173"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDB6"/>
    <dbReference type="GeneID" id="8291059"/>
    <dbReference type="KEGG" id="lth:KLTH0B09856g"/>
    <dbReference type="eggNOG" id="KOG0916">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000844_0_1_1"/>
    <dbReference type="InParanoid" id="C5DDB6"/>
    <dbReference type="OMA" id="LWFWITV"/>
    <dbReference type="OrthoDB" id="1880850at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000148">
        <property type="term" value="C:1,3-beta-D-glucan synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003843">
        <property type="term" value="F:1,3-beta-D-glucan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006075">
        <property type="term" value="P:(1-&gt;3)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051278">
        <property type="term" value="P:fungal-type cell wall polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026899">
        <property type="entry name" value="FKS1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056261">
        <property type="entry name" value="FKS1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003440">
        <property type="entry name" value="Glyco_trans_48_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12741:SF15">
        <property type="entry name" value="1,3-BETA-GLUCAN SYNTHASE COMPONENT FKS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12741">
        <property type="entry name" value="LYST-INTERACTING PROTEIN LIP5 DOPAMINE RESPONSIVE PROTEIN DRG-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14288">
        <property type="entry name" value="FKS1_dom1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23605">
        <property type="entry name" value="FKS1_dom2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02364">
        <property type="entry name" value="Glucan_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01205">
        <property type="entry name" value="FKS1_dom1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="329"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="368"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="406"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="433"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="494"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="547"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="571"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1181"/>
            <end position="1206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1241"/>
            <end position="1265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1335"/>
            <end position="1355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1361"/>
            <end position="1379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1450"/>
            <end position="1472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1492"/>
            <end position="1514"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1526"/>
            <end position="1545"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1557"/>
            <end position="1582"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1594"/>
            <end position="1612"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1632"/>
            <end position="1654"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1689"/>
            <end position="1709"/>
        </location>
    </feature>
    <feature type="domain" description="1,3-beta-glucan synthase component FKS1-like" evidence="12">
        <location>
            <begin position="171"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR21777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1762" mass="203466" checksum="B8B83543976BE015" modified="2009-07-28" version="1">MEDEQFSAWYHDGEAPVTRTELRDIFDDLARKFGFQQSSKENMQQHMLAQLDSRARRSSARTALTSLHASYIGGDEANFKKWYFAAQLDLDEEIGFQNLKLHGKARKRNLKHAKERGVSIEEQIRLWKEQEEEFANSHPKLPFTQTQPKINESFATADYKWKLRMRDLEPGQMARQLALYFLCWGEANQIRFTPECLCFIFKCALDHDVSEADVCQASKPEFSYLDDIITPLYRFLRSQVYHVDETNGKMTRLEKDHKDIIGYDDVNQLFWYPEGIERLVLESGERLIDKPSHERYLSLKDVLWSKAFYKTYKETRTWMHCATNFNRIWIIHLSTFWFFTSFNAPTLYTKDYVQLLNNPPTRQARLSAVALGGTLTCIIQIVATLFEWSFVPRKWPGGQHLTRRMIALLVCLAINFAPSVYVFGFFDLDVHSTYAYTASIIQLIIAIVTTFFFAIRPLGGLFESYLFKGRKKRRYTSSQTFTAQFPKLMGRSRWFSWGLWFFVFLGKFIESYFFLTLSLRDPIRVLSIMDMSRCNGDSLLGSHLCRLQPKITLIIMILTDLVLFFLDTYLWYIICNCIFSIILSFSLGTSILTPWKNIYSRLPKRIHSKILATSEMDVKYKPKILVSQIWNAMIISMYREHLLSIEHVQRLLYQQVDSANMNKRTLKSPTFFVAQDDSTFKSMDFFPENSEAERRISFFAQSLSTPISEPIPVECMPTFTVLIPHYSEKILLSLKEIIKEESTKSRITLLEYLKYLYPTEWECFVRDTKLIAVENCSINNDQGESESEVLKEGLLGVSKEYDDRSKFFQAKIEDLPYHCMGFTNSDPEYTLRTRIWASLRFQTLYRTVSGFMNYSKAIKLLYRIENPTIIQKYGADFELLEEELDRLSREKFRMVVAMQRLKKFDRHEREAAEFLLKAYPDMCISYLEEVPQENGEAIFYSCLIDGHCDFEDTTGERKPQFKIRLSGNPILGDGKSDNQNHSLIFYRGEYIQVIDANQDNYLEECLKIRSILGEFEELDLDQSMPYIPGVDSGGDAPIAIVGAREYIFSENIGVLGDIAAGKEQTFGTLFARTLAEIGGKLHYGHPDFLNGIFMTTRGGISKAQKGLHLNEDIYAGMTAVCRGGRIKHSDYYQCGKGRDLGFGSIMNFTTKIGAGMGEQLLSREYYYLGTQLPIDRFLSFFYAHAGFHLNNLFITMSVQIFFILLINLGSLNHEVIRCEYNKDLPITDLERPIGCYNILPVLHWVNIFVLSIFIVFFIAFAPLLIQELLEKGAWKAFSRLIHHLFSLAPLFEVFVCQIYARSLLTNVTFGGAKYISTGRGFAITRLDFPELYSKFANTSIYAGSKIFLMLLFATVSMWQPALLWFWITVVSMCLAPFLFNPHQFAFTDFFVDYRNFIHWLSRGNSKYERKSWIEHVRSSRAVFTGYKRKIVGDISEESASETRRAQISDVFAAEVIIPLLTAGLSFTAYTFINAQTGVPSATATSAVIRLGIVTFLPIFVNFIVLFVCFQLSFFAAPLLSLCCKKVGATIAAIVHGFAVVIYLITFEVMWCLEGWSFTRALTLLIACISIQNFVFKVITIFFLTREYRNNKSQLAWWSGKWFTTGIGWIAVLQPSRELFVKTIECSLFAGDFILGHLILFVQLPLVFTPFVNYWHSMMLFWMKPKSLISNKLAYTKKQKLRRRRIVQKYLPIHCLILFLFIGLLVAPFYTAKNLPLGPDTFKNSFLDGLIQPKSQDNNDTGTRAPSYILTTTPPMPVFRTVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDE9</accession>
    <name>C5DDE9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">KLTH0C00462p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0C00462g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552248.1">
        <property type="nucleotide sequence ID" value="XM_002552202.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDE9"/>
    <dbReference type="FunCoup" id="C5DDE9">
        <property type="interactions" value="1272"/>
    </dbReference>
    <dbReference type="GeneID" id="8291106"/>
    <dbReference type="KEGG" id="lth:KLTH0C00462g"/>
    <dbReference type="eggNOG" id="KOG0254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_30_1_1"/>
    <dbReference type="InParanoid" id="C5DDE9"/>
    <dbReference type="OMA" id="EMFRANV"/>
    <dbReference type="OrthoDB" id="5141738at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055056">
        <property type="term" value="F:D-glucose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17356">
        <property type="entry name" value="MFS_HXT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000044">
        <property type="entry name" value="Hexose transporter Hxt3p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF75">
        <property type="entry name" value="GALACTOSE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="64"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="147"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="174"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="207"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="240"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="367"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="392"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="432"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="467"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="495"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="11">
        <location>
            <begin position="70"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR21810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="570" mass="63298" checksum="26F5516A2CE3AF82" modified="2009-07-28" version="1">MSSSATPMNNRSNNNTDLAIAGTSTNLDSQSATPSNKMSDDAFDIKGSPAAEAHEIPQKPLSEYILVMCLCLMVAFGGFVFGWDTGTISGFVNMTDFKRRFGDYNTTTEEYYLSNVRTGLIVAIFNIGCAVGGLTLGRLGDMQGRRLGLMAVILVYVVGIVIQISASGKWYQYFIGRIVSGLGVGGIAVLSPTLISETAPKHLRGTCVSFYQLMITLGIFLGYCTNYGTKTYTNSVQWRLPLGLCFLWAIFMIGGMVFVPESPRYLIEKGRFEEAKRSIAKSNKLDTEDSGVLAEVDLINAGVEAEKLAGNARWGELFSPRGKILQRVIMGCVIQSLQQLTGNNYFFYYGTTIFQAVGLDDSFQTSIILGVVNFMSTFVGLYTVEKFGRRKCLLWGSAAMSVCFVVFASVGVTKLYPNGKDMPTSKSAGNAMIFFTCLFIFFFATTWAPIAYVIVSETYPLRVKNRAMAMSVGSNWIWGFLIGFFTPFITSAIGFYYGYVFMGCLVFSFFYVFFFVCETKGLTLEEVNEMYEEGVLPWKSESWVPALRRDESYDVNETHEDGKPFYKRFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDJ0</accession>
    <name>C5DDJ0_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">V-type proton ATPase subunit C</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0C01386g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment. Subunit C is necessary for the assembly of the catalytic sector of the enzyme and is likely to have a specific function in its catalytic activity.</text>
    </comment>
    <comment type="function">
        <text evidence="5">Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments. Subunit C is necessary for the assembly of the catalytic sector of the enzyme and is likely to have a specific function in its catalytic activity. Reversibly leaves the enzyme after glucose depletion, causing the catalytic subcomplex V1 to detach from the V0 section.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the V-ATPase C subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552289.1">
        <property type="nucleotide sequence ID" value="XM_002552243.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDJ0"/>
    <dbReference type="FunCoup" id="C5DDJ0">
        <property type="interactions" value="442"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDJ0"/>
    <dbReference type="GeneID" id="8291624"/>
    <dbReference type="KEGG" id="lth:KLTH0C01386g"/>
    <dbReference type="eggNOG" id="KOG2909">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017554_3_0_1"/>
    <dbReference type="InParanoid" id="C5DDJ0"/>
    <dbReference type="OMA" id="VMIWIHV"/>
    <dbReference type="OrthoDB" id="6605928at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000221">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14785">
        <property type="entry name" value="V-ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000002">
        <property type="entry name" value="V-type proton ATPase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1460.10">
        <property type="entry name" value="subunit c (vma5p) of the yeast v-atpase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1180">
        <property type="entry name" value="Vacuolar atp synthase subunit c, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004907">
        <property type="entry name" value="ATPase_V1-cplx_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036132">
        <property type="entry name" value="Vac_ATP_synth_c_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10137">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10137:SF0">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03223">
        <property type="entry name" value="V-ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118203">
        <property type="entry name" value="Vacuolar ATP synthase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="4 6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00053565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR21851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="392" mass="44210" checksum="67E350A7101DD01D" modified="2009-07-28" version="1">MATALHTANEFVLLSLPMNAQPASAPGKSATSWLHESLVGGRTFVSEFHIPEFKIGSLDTLVVQSEELAKIDSQIGSSVAKILEILGNLTESQTNAYKTVPIGNVPIPEYLENFQWQTRKFKLDRSIKELIEEISRESFQLDADVRATSTNYSNAKTNLAAAERKRTGDLSVRSLHDIVKPSDFILDSEYLTTVLVAVPKNLQDDFQKSYESLAKNVVPCSASVLKRDDDYVLFNVHLFKKSVNEFVSACRERKFIPREFEYSEELINQLTKEHDSAAHLEQSLRVQLVRLAKTAYSDVFMNWFHIKALRIFVESVLRYGLPPHFNTSLIAVPPKTLSKCKAELVQQFGYLGGNAFSKDKAGKIKQQDSSLHEYASLVDTEYEPFVMYTVEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDK0</accession>
    <name>C5DDK0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0C01628p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0C01628g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552299.1">
        <property type="nucleotide sequence ID" value="XM_002552253.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDK0"/>
    <dbReference type="FunCoup" id="C5DDK0">
        <property type="interactions" value="241"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDK0"/>
    <dbReference type="GeneID" id="8291158"/>
    <dbReference type="KEGG" id="lth:KLTH0C01628g"/>
    <dbReference type="eggNOG" id="KOG0504">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1161">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2421">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002842_1_0_1"/>
    <dbReference type="InParanoid" id="C5DDK0"/>
    <dbReference type="OMA" id="LCTALNW"/>
    <dbReference type="OrthoDB" id="1577640at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08578">
        <property type="entry name" value="GDPD_NUC-2_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14483">
        <property type="entry name" value="SPX_PHO81_NUC-2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057506">
        <property type="entry name" value="C2_GPCPD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050889">
        <property type="entry name" value="Dendritic_Spine_Reg/Scaffold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24166:SF48">
        <property type="entry name" value="PROTEIN VAPYRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24166">
        <property type="entry name" value="ROLLING PEBBLES, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25329">
        <property type="entry name" value="C2_GDE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03105">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="SPX" evidence="4">
        <location>
            <begin position="1"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="393"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="501"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="536"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="domain" description="GP-PDE" evidence="5">
        <location>
            <begin position="785"/>
            <end position="1089"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR21861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1089" mass="122426" checksum="D40D35F9A9BA4140" modified="2009-07-28" version="1">MKFGKHLETRQLALPEYNGHFIDYKSLKKLIKQLSLPAVGSNGILETGDESLVHQVLQEHKASFFFRLERELEKVNAYYLEKEADLRIKFDILRLRFEEFEKRGKLASKNTVSYRHLRDGIKKFERDLAHLEQFVELNRTGFSKVLKKWDKRSHSHTKDFYLATVVSVQPVFTRNEASELNDAVSVILMQLNEIGNSEDFFSGSFNARTVSTSYARVQDIDPVASSVASYSLSGGFKPQTEPDFFDIGTEIESWYLEALSIGKLKDEEPRWTLLRDFPQQKVQAFVESHVPHSRIDKNLIVRDALTKIFLLLVSSSIDDESLRVFFESAGSSIDLSYTEEDDVVFSKRNIFHEAAICEPHSRGFILLRALDQCKGSASFESTLRTLLNAQDANGRTPLHYACDLGKMEFVKLLLNSNLLDSVDILDNDSKTPLVLSVIKNQPEITEALLVLGHANPSPSVKDSGKPQFAPLNVACAYQNFAAAKLILDFSNFDLSAVQDSHGLCPLHIVAKNGGDAKMIELLVSHGSDPNRIDGFNGWTPVFYAIQEGHRNTVEELLKHGASIDIYDEDNLSPYFYALWEGHLSVVNLLRGHCSQKTDTTNPSMDPLRSVLEPTTVMRLEDSLHGIPDFTLPPPIIPLRKYGHNFLEKKIFVNLSFRAGTASIVLNKEDEMVLSSPGRITLTSNVSDIIPRNVILPTEDDDENFVVFQVDSLDGFSIDFEIFPSFGTRMIAKTTAMSSLFKGHDKQPSSKGAFILPLFDARLKNVGELVLDYTFIFPYSGRPLEITKYETYWKSTTGNETGKSGNQFITSSLSGTYTNFFLFPLNDGTLIVSPKKAFSTGPMDLKIFDLNANQLEKLMGYSLQGNHEGSKEVTYEQLLNERFTTLETLLNNTPPNAKLEIEVCFPTACEFESIPLKLSPLVNINDFIDNVLSSSFAHARQRFHNGMTSSIVFTSRNPEVCSILNWKQPNFPVLFYMNGLRKENGVLVKDTPHHLSHLSMDPEKVNYKEPCSRCIREAVQFASYNNLLGVIVPLELLRSSQQLVSEIRGRGLLVIGSLFEGENIAVDLAGEDINGVRTHDTLQFKGSIDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDL3</accession>
    <name>C5DDL3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0C01936p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0C01936g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552312.1">
        <property type="nucleotide sequence ID" value="XM_002552266.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDL3"/>
    <dbReference type="FunCoup" id="C5DDL3">
        <property type="interactions" value="51"/>
    </dbReference>
    <dbReference type="GeneID" id="8291628"/>
    <dbReference type="KEGG" id="lth:KLTH0C01936g"/>
    <dbReference type="eggNOG" id="ENOG502REX9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026020_0_0_1"/>
    <dbReference type="InParanoid" id="C5DDL3"/>
    <dbReference type="OMA" id="RMKSIYQ"/>
    <dbReference type="OrthoDB" id="4035953at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051694">
        <property type="entry name" value="Immunoregulatory_rcpt-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014805">
        <property type="entry name" value="SKG6/TOS2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15549:SF30">
        <property type="entry name" value="MID2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15549">
        <property type="entry name" value="PAIRED IMMUNOGLOBULIN-LIKE TYPE 2 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08693">
        <property type="entry name" value="SKG6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="24"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="643"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="26"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="669"/>
            <end position="678"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR21874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="678" mass="74176" checksum="FDCB301AEB1CE63D" modified="2009-07-28" version="1">MITTSIERPTTTVFLAPPRIFRRADSSTNSGSSTSESSSKASSTKCGNGSKCTLPADRTESATVAVAVVVPVVVVILVLGFVLWKVWRRSRKEAMEDNDPDFDGDGEYMPAANAGYELKENSVPTSGSNFDDYEFKSQGYPADAGYAGSNYYKPPIVDPFQLPMSDDAGDLRTFARSIQNNEFDGYRLASHSASELSLSNPTGGFHRPQTFIQQGRSGSFTSSNLMSVESADNVARINDGHIQDSMPQATQAEEMASGESGEYASDVENSPIKNARAEVAQGYIGDSAGLDPEKSFKGSASIENDKASSNQSFVDACLNVSKTGMEKGSEYDDDYEIHNSKEEENIKRMKSIYEVYLDRNGTVRTVNPPSKETEEELGAHEEGFAASESLPAGASFQIHEAAERQKQLDASNAGVVRDELQLPGQGEETVARTRAASSIYSELPAMPQQTAQANQYRRQAPYEQPAQYEYQPEYYNDPNAVYSYQNQAYMPQQYHHPQALENIEELPNPSSLPFSATTSSLTSYNKKAGRSLTPGGMPVYNGTKVNPIDHPELFYSQSSLVDEQPAYAQQTPSQGQVLPHHLRQSVVMTNPAQLSHPTLFKPAGSFRNFSAANSRNNSMTSQHSVQQHQTQLAHQRVSGILDDHDTVQPPRLGGILPHSGSHDDLRRQLGSSDNYNVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDS5</accession>
    <name>C5DDS5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">UV excision repair protein RAD23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0C03410g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Involved in nucleotide excision repair.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the RAD23 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552374.1">
        <property type="nucleotide sequence ID" value="XM_002552328.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDS5"/>
    <dbReference type="FunCoup" id="C5DDS5">
        <property type="interactions" value="1066"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDS5"/>
    <dbReference type="GeneID" id="8291238"/>
    <dbReference type="KEGG" id="lth:KLTH0C03410g"/>
    <dbReference type="eggNOG" id="KOG0011">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040364_0_0_1"/>
    <dbReference type="InParanoid" id="C5DDS5"/>
    <dbReference type="OMA" id="PHMLEPI"/>
    <dbReference type="OrthoDB" id="419317at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14378">
        <property type="entry name" value="UBA1_Rhp23p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01805">
        <property type="entry name" value="Ubl_Rad23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000002">
        <property type="entry name" value="UV excision repair protein RAD23 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000003">
        <property type="entry name" value="UV excision repair protein RAD23 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.540">
        <property type="entry name" value="XPC-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004806">
        <property type="entry name" value="Rad23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015360">
        <property type="entry name" value="XPC-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036353">
        <property type="entry name" value="XPC-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00601">
        <property type="entry name" value="rad23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10621">
        <property type="entry name" value="UV EXCISION REPAIR PROTEIN RAD23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10621:SF0">
        <property type="entry name" value="UV EXCISION REPAIR PROTEIN RAD23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09280">
        <property type="entry name" value="XPC-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01839">
        <property type="entry name" value="RAD23PROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101238">
        <property type="entry name" value="XPC-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3 5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="8">
        <location>
            <begin position="2"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="7">
        <location>
            <begin position="139"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="7">
        <location>
            <begin position="348"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="76"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="185"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="79"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="185"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="210"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU367049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR21936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="391" mass="42381" checksum="36254A80618C3CAD" modified="2009-07-28" version="1">MVKINFKDFKKQVLALELDSGSTISEAKQRLAENKDCDESQIKLIYSGKILQDDRTIADYKLNDGDQVIFMISKKKSTTTKTTVPPSEQKPAEPSAPSSSAASAPSGENAGTSEQTSAPAAAAPASSTAPTDPAFVTGSRRDETVQRIMEMGYDREQVERALRAAFNNPDRAVEYLLMGIPEHLQQTQQPQQQQQQQQQQQQQPEPQAQESHETQQPQEHEEQQASSDDLFAQAAASAGGEEGGDSARAPGTIGLTMEDLLSLRQVVTGNPEALPPLLESLSTRYPELREQIMTNPEMFISMLLEAVGGSLPEGIMEGDAGMEAGAEGALAGADVEGAEQAPQLEISPQDQEAISRLCELGFERTLVVQVYFACDKNEEIAANMLFSEYAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDU2</accession>
    <name>C5DDU2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0C03806p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0C03806g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552391.1">
        <property type="nucleotide sequence ID" value="XM_002552345.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDU2"/>
    <dbReference type="FunCoup" id="C5DDU2">
        <property type="interactions" value="40"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDU2"/>
    <dbReference type="GeneID" id="8291255"/>
    <dbReference type="KEGG" id="lth:KLTH0C03806g"/>
    <dbReference type="eggNOG" id="KOG0254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_27_4_1"/>
    <dbReference type="InParanoid" id="C5DDU2"/>
    <dbReference type="OMA" id="MLPWTGM"/>
    <dbReference type="OrthoDB" id="2130629at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17476">
        <property type="entry name" value="MFS_Amf1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000397">
        <property type="entry name" value="Aminotriazole resistance protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718:SF14">
        <property type="entry name" value="AMINOTRIAZOLE RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY MULTIDRUG TRANSPORTER MFSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="90"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="152"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="181"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="210"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="246"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="277"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="315"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="349"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="380"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="410"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="482"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="53"/>
            <end position="506"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR21953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="525" mass="58067" checksum="4E3290D8BDBE4464" modified="2009-07-28" version="1">MNSEYTPVSKQRTGSDISRKVVDNHEDSDMLLASRSRLQNPDYFDKQWKEYLFVISSMLSQLLNQAAALQANPLFDLLSEDLKAKASQQAWLMASFPLVSGSFILVSGKVGDIYGLKKTLLGGYAVVVIWSLICGLSSYAHNVDFFIVARSFQGLGIAFVLPNVTGIVGNIYKPNTKAKNMVFSMIGACAPIGATLGCLFSGLIGTESEAWAWTFYAYTIVAALNGLIAWYVIPDNIPTNVHKFKMDWLGSFLGVCGLIMFNFVWNQAPTDGWDSAYIIVLLVISVILLLIFIIFEARFAKEPLLPRAVLFNRKLMMILASLFLGWGSFGIWTYYYMAFVLNLRHYTPLWAGGSYFMFAIFGTIAAFLVGHTIHKTGPAIILLASVIAFDVGCIMLSVTPVDQTYFRMSLGTMVILSFGMDLSFPASSIILSDQLPSQYQGMAGSLVNTMINYSMSLCLGVSGTAERQVNKDGTDLLKGYRAALYVGIGLASVGIFVSGAYAIEDWWTRRRERSVRIKLDEKHQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDY9</accession>
    <name>C5DDY9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glucan endo-1,3-beta-D-glucosidase</fullName>
            <ecNumber evidence="3">3.2.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0C04906g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;3)-beta-D-glucosidic linkages in (1-&gt;3)-beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.39"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 81 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.39" evidence="3"/>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552438.1">
        <property type="nucleotide sequence ID" value="XM_002552392.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DDY9">
        <property type="interactions" value="235"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDY9"/>
    <dbReference type="GeneID" id="8291305"/>
    <dbReference type="KEGG" id="lth:KLTH0C04906g"/>
    <dbReference type="eggNOG" id="KOG2254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005482_0_0_1"/>
    <dbReference type="InParanoid" id="C5DDY9"/>
    <dbReference type="OMA" id="DNTIHPK"/>
    <dbReference type="OrthoDB" id="4473401at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052861">
        <property type="term" value="F:endo-1,3(4)-beta-glucanase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.98.30:FF:000006">
        <property type="entry name" value="Endo-1,3-beta-glucanase Engl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1170">
        <property type="entry name" value="glycoside hydrolase family 81 endo-[beta] glucanase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.30">
        <property type="entry name" value="Golgi alpha-mannosidase II, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.420">
        <property type="entry name" value="Immunoglobulin FC, subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005200">
        <property type="entry name" value="Endo-beta-glucanase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040720">
        <property type="entry name" value="GH81_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040451">
        <property type="entry name" value="GH81_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31983">
        <property type="entry name" value="ENDO-1,3(4)-BETA-GLUCANASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31983:SF20">
        <property type="entry name" value="GLUCAN ENDO-1,3-BETA-D-GLUCOSIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17652">
        <property type="entry name" value="Glyco_hydro81C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03639">
        <property type="entry name" value="Glyco_hydro_81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52008">
        <property type="entry name" value="GH81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950336" description="glucan endo-1,3-beta-D-glucosidase" evidence="10">
        <location>
            <begin position="18"/>
            <end position="1281"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 81 N-terminal" evidence="11">
        <location>
            <begin position="583"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 81 C-terminal" evidence="12">
        <location>
            <begin position="916"/>
            <end position="1268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="135"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="197"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="423"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="507"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="142"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="172"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="197"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="210"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="265"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="291"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="433"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="511"/>
            <end position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF17652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR22000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1281" mass="132815" checksum="22F216E105214E47" modified="2009-07-28" version="1">MLSALQVLALLLAGTHAKNSSHPIYSNSSAIVHETDRTLALNYTTLDYGEPYVDAATSRPARFSASQAAASATSASLSHVTVAGDTPLPASTAGAETIYTVSAGPGSTGTGASLAASAPGRATASSKYSAASSGVVSGGSDGFSSDLPSGSQKPAPSAADDAAATQGASQARDSSTVSTPSLGTSFAADSSETFAHSSDASMLSSRETPAHGSVGSSTSSSHETSAHGSGVFPTPSSSQAPAHSSDASSALSSLQTSADGARETPAQSSDASSTLGSSQTPARYSGTSQLRSGAASTETASSEGTRSAAHSASSTARFQTLSSSSFSPVSELLTATASTSTSVLYSATSPYTSATAKGSIVTSVPSHSANPLVTQSLSSSKLSQSISTTYTASKTVDTESLLPASNFTSSSVVIGSSSNSSASATGTSLSAPGQLNASASQGTTSSPARSSVILSTSVVTHSTVNTTTMVVPVTSTYSNRTTVLSKPTTARFTLVGTSNVVSNQTATLQPSGASSSSLATPSASSSAYTSSSSSAAMTTESTASSTSETPVVSIVSETVTTVDLFQAVATDEPPSVFARHSNPMDLSSGVSNNGSPYGTNKFYTNLIVGDQTSAAFVYPYSVWKYSSNGVNGFAVSHTSKDQYSYGNYDSDGNSEYLVNPLGIASLIFSAESFGSACNMYVSDMTTSSCDVTINDGSVSNILQVPLVQGMGFASGIYHGSLKPEIRTSAAFISLEQQTSDILPAGVQKYRVGLNSGSTWLVYVTLPDGYSDDDFTLSFSDSSTLSGSQAIDGLIVQFAAAPEDSTYDSFYDNAAGMYATDFKLEGSSDGASASYSFDYQTEGRSASGKTMIFALPHHLDALSAESTAARTGVQLQSTTKGTMEALLATSLVFTEILNTQLGWLPWTSQLGSRNLTYSAEQLQLLAETANDEINIDVWGSVGGLNTYYLGKVLDKYAYILLTVADVLQDSAVTAAALENLKQAFAKLMQNQQLYPLYYDTKFGGVVSSGDWASTATGYDFGNTYYNDHHFHYGYIVHAAAVVGHVDAQQGGSWAQDNKAWINTLVRDVANPSAADAYFPVSRMFDWFHGHSWAAGLFANANGKNQESSSEDYNFAYGMKLWGAVVGDRAMERRADVMLAVMQRSMNAYYLFADDNAVEPSEILGNKVAGILFDNIIDYTTYFGTETQYKNGIHMIPVTPASGVIRGPTFVQQEWEEKLASVAATLTDGWGGLLRLNQALYDPASSYAFFADSGFSSAALDNGLSRTWALAFSGGLANSLGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DE15</accession>
    <name>C5DE15_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0C05500p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0C05500g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat mio family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552464.1">
        <property type="nucleotide sequence ID" value="XM_002552418.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DE15"/>
    <dbReference type="FunCoup" id="C5DE15">
        <property type="interactions" value="858"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DE15"/>
    <dbReference type="GeneID" id="8291331"/>
    <dbReference type="KEGG" id="lth:KLTH0C05500g"/>
    <dbReference type="eggNOG" id="KOG1008">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005843_0_0_1"/>
    <dbReference type="InParanoid" id="C5DE15"/>
    <dbReference type="OMA" id="YEPTGHA"/>
    <dbReference type="OrthoDB" id="341486at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16691">
        <property type="entry name" value="mRING-H2-C3H3C2_Mio"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037593">
        <property type="entry name" value="MIOS/Sea4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049092">
        <property type="entry name" value="MIOS_a-sol"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031488">
        <property type="entry name" value="Zn_ribbon_mio"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16453:SF9">
        <property type="entry name" value="GATOR COMPLEX PROTEIN MIOS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16453">
        <property type="entry name" value="WD40 DOMAIN-CONTAINING PROTEIN MIO FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21719">
        <property type="entry name" value="MIOS_a-sol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17034">
        <property type="entry name" value="zinc_ribbon_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="MIOS-like alpha-solenoid" evidence="6">
        <location>
            <begin position="470"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="domain" description="GATOR2 complex protein MIO zinc-ribbon like" evidence="5">
        <location>
            <begin position="888"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="410"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="410"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="420"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF17034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF21719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR22026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="985" mass="110971" checksum="972C4162F93C297B" modified="2009-07-28" version="1">MGLIKRALNWSQDGYYDYLSVNPTREEVAHYRVDPESDSDQSISKLTVVKDFDNITCLDFSETEMGLVGVGEKNGMVRVMNMIYGFSGEDNSQLRVRAKQQRAVNALGISPNGMVAIGLDRNRHDPSLQVWDVNYQSTNSNIVNPSFSYCMNESVISLKFLNDTTLITATTKLLKEVDLRAPNPSYQHPTRLCNDIKVNPFNHWQFTTYSDDGTLAIWDRRRLGHSSTSSDSLEAQSLLCFDKLMGTGAASRRYANSCFRWSAVRSNEFTTLHGGQVIRRWRLGSFPISIADEKQSEDSEFPFELDSLFISTVHDIKTTFDRVTTFDYIPRSNGRTNFICMRQSGTIYRMSLKGGMSRAVFNSDNNMLMTNNDLSEATELRFADTNDKINTDKTSTSLKNLSFEDLDISDEDYQPEEETSNGFSSDTENSEASSKASFEQDIEGHPLSDQGYQVLLKPEKLLRNDISMIMKRRALLNYGLDPVKTVELIDSSKALQNNAYIRNTWRWLSIAKASVDDGTMVSGELDLGYEGILGIWNGLDGLSKQNRCREGAILTEKQLNRELEKIIKMRGKNKSRMGESKGLLTFTNSNKTVQRKLCMVISGWDLNPSDYEEKYRNLIKANHYEKAAGWAVFFGDVTKAIEILSSAKKERLRLIATAIAGYLAYKNQPGNNAWRQQCRRMSSDLDDPYLRVIFGFIADNDWWDILSESAISLRERLGVALRFLNDKDLTRFLERTACTVIENGELEGLILTGITPSGIKLLQSYVNKTSDVQTAALLSIYGCPRYFKSLQVDEWIGTYRAMLNSWELFTARAKFDVLRGKMSRTSRNPAPQGARPRQLYIQCLKCNKNINNPVDPSVSRGSTSGGMMKNFVANKKFGFNNASPSGNSHQKHSCPHCGTPFPRCAICLLPLGTSNLPIVINGGTIEPSPDRGSTESLAESRRLRLNEWFSFCLSCNHGMHAGHAEEWFEKHYICPVPGCVCHCST</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>C5DE43</accession>
    <name>C5DE43_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0C06138p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0C06138g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552492.1">
        <property type="nucleotide sequence ID" value="XM_002552446.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DE43"/>
    <dbReference type="FunCoup" id="C5DE43">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="GeneID" id="8291360"/>
    <dbReference type="KEGG" id="lth:KLTH0C06138g"/>
    <dbReference type="eggNOG" id="ENOG502R9IN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036649_0_0_1"/>
    <dbReference type="InParanoid" id="C5DE43"/>
    <dbReference type="OMA" id="SSGYCYV"/>
    <dbReference type="OrthoDB" id="3365224at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039931">
        <property type="entry name" value="EEIG1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019448">
        <property type="entry name" value="NT-C2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21456:SF1">
        <property type="entry name" value="C2 NT-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21456">
        <property type="entry name" value="FAMILY WITH SEQUENCE SIMILARITY 102"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10358">
        <property type="entry name" value="NT-C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51840">
        <property type="entry name" value="C2_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2 NT-type" evidence="2">
        <location>
            <begin position="5"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="128"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="57"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="135"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR22054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="422" mass="46933" checksum="C14377D9D01DA552" modified="2009-07-28" version="1">MKSFANKSKSRRPKFLFNLKINELTNIPQSSGYCFVKWHLKDGTGTTSHTIIPEIGSPQSNVKSSSQSRGATPRVLVKSHRAHWNYSLQKPVQVKLVTDKNKNLNSKEMVLEVYFEFLEPTNASHGKFQHSRQLSDASSTNSSASSSNSNVYTQKVSRKMLLGVVEVNIAEYVNREQHMVTSRFLLQKSKVNSILSLGIQLELTRGSFDDFQLPSAVSSGQLPNTFRNGITEVFDESSDISSPVSSTFLGNSGASGSNYNGSSSPAKKTADLSGSFSIAISNPFVEKLYQKTFQVPWDPRPGEFTPKECVEDILDGGDGWAKNEKGINLIDIDALHLAELERDYDAQLKGVPMNMNYGEFDKRAFLERRVGYETHTSTQDRGIGGRKKLDKSSDNLCEDNLDDYDTAKPNDFKSWTIGKVLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEA2</accession>
    <name>C5DEA2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0C07524p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0C07524g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552551.1">
        <property type="nucleotide sequence ID" value="XM_002552505.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEA2"/>
    <dbReference type="STRING" id="559295.C5DEA2"/>
    <dbReference type="GeneID" id="8291422"/>
    <dbReference type="KEGG" id="lth:KLTH0C07524g"/>
    <dbReference type="eggNOG" id="KOG1347">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012893_1_2_1"/>
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    <dbReference type="OMA" id="MCENTEA"/>
    <dbReference type="OrthoDB" id="2126698at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13132">
        <property type="entry name" value="MATE_eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045069">
        <property type="entry name" value="MATE_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="40"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="74"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="117"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="183"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="215"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="255"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="299"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="340"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="380"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="413"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="438"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR22113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="482" mass="52651" checksum="70433DB6F086545C" modified="2009-07-28" version="1">MARLIEETPRHNYASIPGSAAPEEVEVSYRDEFRQIGKNALPLIVTFALQTSLSFVTVAFVGRLGALELGGVSLANVTFTATSAVFQGLATCLDTLCPQAFGAKQYQLVGLYFQRGLAISLVFACPIALLWWYSELMLGAMVDDQRLVKIAARYLRVMVTSIPGYVTFECGKKYLQAQNDFTTAQYILFVCAPLNVLLNYLFVFRFGLGFVGAPMATSLTFTLMGASLATFIWCKTYRDGTTSCWSPLKNWKPIFANWGTMVSLAIPGIIMIEAEFLAFESLTVLSAKFGTESLAAQSVIASIQSLTFQAPFSAGVAASNRIAYHIGKSRVRACQVASRATLIYIGFLIGTANLLFLVLGRTIIPSVFSNDEGVIKIASQVLPIIGINQVCDVLNVLSAGILRAQGRQKIGGVLNIIAYYLVGLPMAIFLGFRCKWALQGFWVGLGCGILFLGLSELYCVWKSDWAKIIRNSRRLHKDSPAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEC6</accession>
    <name>C5DEC6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0C08096p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0C08096g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552575.1">
        <property type="nucleotide sequence ID" value="XM_002552529.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEC6"/>
    <dbReference type="FunCoup" id="C5DEC6">
        <property type="interactions" value="298"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEC6"/>
    <dbReference type="GeneID" id="8291448"/>
    <dbReference type="KEGG" id="lth:KLTH0C08096g"/>
    <dbReference type="eggNOG" id="KOG1505">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041844_3_2_1"/>
    <dbReference type="InParanoid" id="C5DEC6"/>
    <dbReference type="OMA" id="FYIREFR"/>
    <dbReference type="OrthoDB" id="189226at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036149">
        <property type="term" value="P:phosphatidylinositol acyl-chain remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07990">
        <property type="entry name" value="LPLAT_LCLAT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032098">
        <property type="entry name" value="Acyltransf_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10983">
        <property type="entry name" value="1-ACYLGLYCEROL-3-PHOSPHATE ACYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10983:SF16">
        <property type="entry name" value="LYSOCARDIOLIPIN ACYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16076">
        <property type="entry name" value="Acyltransf_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="13"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="58"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="5">
        <location>
            <begin position="106"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR22137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="400" mass="45449" checksum="87DDD256BC875CB6" modified="2009-07-28" version="1">MSVSFLAQRAVKLLVTLASIVIFIQGAFVIVTHQVLTKTFLTHDPIRMQKHLDRSKKSFIILLITILTIVAPSGVRITTDNRSIPKNTMVKDLRTGGIISSMKQKSVLISNHQIYTDWVFLWWLAYTGDLAGNVYIMLKNSLAKIPIMGYGMRNYNFIFMNRKWSKDRINLANHLGNLDHNARGVGRLAGKHPSLDIASGKEVWSDNGAATKDHRQIRWPYTLIIFPEGTNLSANTREKSRVFSEKANLPIFNNLVLPRVTGLRFSLQELRNSCEVVYDTTIGYSGVKQNEYGQDIYQLSNIFLRGHSPDLVDIYIRAFNLKDIPLDDEEEFTKWLLDVWHEKDKLLDTFYAKGSFDLDPNTHQVTTGTCKIKSSEVAQFLILPLLSLAALVAKILRSFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEF4</accession>
    <name>C5DEF4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0C08734p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0C08734g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552603.1">
        <property type="nucleotide sequence ID" value="XM_002552557.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEF4"/>
    <dbReference type="FunCoup" id="C5DEF4">
        <property type="interactions" value="1139"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEF4"/>
    <dbReference type="GeneID" id="8291477"/>
    <dbReference type="KEGG" id="lth:KLTH0C08734g"/>
    <dbReference type="eggNOG" id="KOG1156">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006686_1_1_1"/>
    <dbReference type="InParanoid" id="C5DEF4"/>
    <dbReference type="OMA" id="HTAINYD"/>
    <dbReference type="OrthoDB" id="10263032at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031415">
        <property type="term" value="C:NatA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.1040:FF:000003">
        <property type="entry name" value="N-terminal acetyltransferase A, auxiliary subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1010">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021183">
        <property type="entry name" value="NatA_aux_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22767:SF2">
        <property type="entry name" value="N(ALPHA)-ACETYLTRANSFERASE 15_16, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22767">
        <property type="entry name" value="N-TERMINAL ACETYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12569">
        <property type="entry name" value="NatA_aux_su"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000422">
        <property type="entry name" value="N-terminal-AcTrfase-A_aux_su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="646"/>
            <end position="673"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR22165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="849" mass="97882" checksum="0B532A07961622AB" modified="2009-07-28" version="1">MSRRKGALPLKANGSAVGRSKEDNNLIEALKLYESKTYKKSLKLVDAILKKNSQHIDSLALKGLNLYCTGQKEDAANYVQKAIARIESSGASPIGCHILGIYMRNEKRYSEAAKWFQSSLDNGSTNKQIYRDLATLYSQEHDYKNLLKARKLYWEEFMGYRANWTSLAIAHELNGQLDESANVLTKFEELAKGKLGESEAYEHNECLMYKNDVLYKAAGADHEKLKGVLKHLDEIEPDVFDKYGWLERRAAIFMKLNQKGDASKIYRTLIKRNPDNFKYYKLLEIALGIQRDAKLRLALYKKLESFYPRAEPPKFIPLTFIEDNSCFAESLSSYILPQLKRGVPATFNNIKTLYKKDVMRNSAIAEEIVLKYFDSINPKEAPIPYVWTCYFMCQHYLYLKSFQKAREYIELALAHTPTLVELYILKARVLKHTGLLAEAAETINEGRKLDLQDRFINTKTVKYYLRANMVEKAVEVVSIFTKNDGAANGVMDLHLMEASWFIAEQADAYFRLYLEKKRLLHDEKSMEVPEDEESQESHARHIRDLTYETTKFGGLALKRWDAISKFYKQFEDDQLDFHSYCMRKGVPRAYLEMFQWGKTVYTLPMFVRAMKGAARMYFILNDEMKASSTTPEDDAIGELVGAKKGNKKTKKEAAAIKKRIEEDKKTVAAYEKDEDPLGEAYIFTKAPMQDFYNKFYRRFSEEATESSKDYILDFEYHIRCGKLALCVGAISKYADMFGKKDSIVGAMTLCLLDKIKFSNEIDEVTKKIAIKGLERLLSELPLKQLDEEAFDWLSFFQQEFEGPSLESLLLIHRSSVKIISAENIKQQVIEKLREEEPAVQSSVLNYQLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2023-06-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEM6</accession>
    <name>C5DEM6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0C10494p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0C10494g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552675.1">
        <property type="nucleotide sequence ID" value="XM_002552629.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEM6"/>
    <dbReference type="GeneID" id="8291556"/>
    <dbReference type="KEGG" id="lth:KLTH0C10494g"/>
    <dbReference type="HOGENOM" id="CLU_2513021_0_0_1"/>
    <dbReference type="InParanoid" id="C5DEM6"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR22237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="85" mass="9756" checksum="E2731E3AA0681012" modified="2009-07-28" version="1">MVEGTVYFLLAQPLNSEKVLTTAMIELVYTRVSTLSGAYEVPQVVLADWEQSMRAILRSIDELHLNRVYRTRYAFVQIFKNSTVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>C5DES1</accession>
    <name>C5DES1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">KLTH0C11616p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0C11616g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCB family. Mitochondrial peptide exporter (TC 3.A.1.212) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552720.1">
        <property type="nucleotide sequence ID" value="XM_002552674.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DES1"/>
    <dbReference type="FunCoup" id="C5DES1">
        <property type="interactions" value="591"/>
    </dbReference>
    <dbReference type="GeneID" id="8291607"/>
    <dbReference type="KEGG" id="lth:KLTH0C11616g"/>
    <dbReference type="eggNOG" id="KOG0058">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_84_3_1"/>
    <dbReference type="InParanoid" id="C5DES1"/>
    <dbReference type="OMA" id="MYTGHTL"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015421">
        <property type="term" value="F:ABC-type oligopeptide transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090374">
        <property type="term" value="P:oligopeptide export from mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18573">
        <property type="entry name" value="ABC_6TM_ABCB10_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03249">
        <property type="entry name" value="ABC_MTABC3_MDL1_MDL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000218">
        <property type="entry name" value="Multidrug ABC transporter ATP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394:SF2">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002773">
        <property type="entry name" value="ABC_prm/ATPase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="170"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="281"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="11">
        <location>
            <begin position="125"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="455"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="70"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="74"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR22282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="740" mass="81158" checksum="46B4D644033A97C8" modified="2009-07-28" version="1">MLLARFSRQQGGARLPLGVQLLSWRRGLSGVQAFIHLSPARYHGATHRPLGLLQRPTQLDLRNPVSSSFIRYHSQNSPNSSTPSSSNDSGGKANNESTEEKSKTNYREILRLIRLARHDWKLLSVAIGLLTISSTVGMTIPKVIGIVLDAVKNASGGFSGPMTLEDLPPIIYGLGMFDFLAIFAGILVFGTIANFGRIVLLRVLGEKLVARLRSNVIKKIIHQDAEFFDRNKVGDLISRLSSDAYVVSRSMTQNVSDGFKAIICGGVGIGMMINISASLTSALFLFAPPLLVGALIYGKKIRMISRELQESTGNLTKVSEEQLSGVKTIQAFVAEGKELHRYNNAIRKVFSFGKKEALTNATFFSSTGILGDLSFLIVLAYGSHLVLQGGLSVGDLTAFMLYTEYTGSAVFGLSNFYSELMKGAGAASRLFEMTDHTSQIKPSSGKPYVPSWGEIKFEDVSFSYPTRPGNQIFNKLNFTIEPGSNVCVVGPSGRGKSTIASLLLRYYDPNSGRIMIDGQNISKLSCKSLRRHLGVVQQEPMLMSGSIRYNLTYGMNSEVTMDEIRSVAKKCFCHGFITKFPEGYETVIGPRGASLSGGQKQRIAIARALIKKPKILILDEATSALDVESEGAINYTLGRLMRSRELTIISIAHRLSTIRRSENVIVLGNDGSVVEMGKFKELYANDQSELFKLLNDKKPTQLQSQSRNSVETKENEVDDQVLEQVANDFNLDQKSSKLTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEU7</accession>
    <name>C5DEU7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0D09900p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0D09900g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beclin family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553140.1">
        <property type="nucleotide sequence ID" value="XM_002553094.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEU7"/>
    <dbReference type="FunCoup" id="C5DEU7">
        <property type="interactions" value="568"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEU7"/>
    <dbReference type="GeneID" id="8295379"/>
    <dbReference type="KEGG" id="lth:KLTH0D09900g"/>
    <dbReference type="eggNOG" id="KOG2751">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024219_3_0_1"/>
    <dbReference type="InParanoid" id="C5DEU7"/>
    <dbReference type="OMA" id="EWDVYKA"/>
    <dbReference type="OrthoDB" id="20368at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034271">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034272">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043548">
        <property type="term" value="F:phosphatidylinositol 3-kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045324">
        <property type="term" value="P:late endosome to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.40">
        <property type="entry name" value="Autophagy protein 6/Beclin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007243">
        <property type="entry name" value="Atg6/Beclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038274">
        <property type="entry name" value="Atg6/Beclin_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041691">
        <property type="entry name" value="Atg6/beclin_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040455">
        <property type="entry name" value="Atg6_BARA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12768">
        <property type="entry name" value="BECLIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12768:SF4">
        <property type="entry name" value="BECLIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04111">
        <property type="entry name" value="APG6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17675">
        <property type="entry name" value="APG6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Atg6/beclin coiled-coil" evidence="4">
        <location>
            <begin position="132"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="Atg6 BARA" evidence="3">
        <location>
            <begin position="268"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="191"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF17675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR22702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="457" mass="51452" checksum="48AFB41BCB2E6B50" modified="2009-07-28" version="1">MGDDEVLKCQNCQVPLEIDSSLIDLSLAQRSLLMNTATEPSVPTGIIPPDRLRRLGSVKKGGSLNLQPSVLNSLDSYVFLGGESSTLQQTRWDGESGDEADDSATTKTLSSRVGTLTNIFNVLSSKSNIDYPVCQDCCDLLIQKLKGEYEEALKERDTYSEFLYRLKKQRELESYATSKPHMVSESGGVDTKKEQEELLNELIELEKTEAELDSTIEDLESELQAKNDQEIAELEVMNSRDLEKLEFMSELQSLKNQYESTLNNLDKLRKTNVYNETFRISHDGPFGTINGMRLGGLDDKPIPWQEINGALGQVILLLATICARLKFKLNGYVLRPMGSFSKIEKLDPKDQEWHSYDAFSDGSFKLGRFFHKETSFDRAMVSILEVISQITSRLSMERSSDSEEIELPYNMHGDKINGMAVKLCGNKPGLEWTTACKFVLTNCKWLLAFSSSMINDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEX1</accession>
    <name>C5DEX1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">[acyl-carrier-protein] S-malonyltransferase</fullName>
            <ecNumber evidence="1">2.3.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0D10450g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>holo-[ACP] + malonyl-CoA = malonyl-[ACP] + CoA</text>
            <dbReference type="Rhea" id="RHEA:41792"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="EC" id="2.3.1.39"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.3.1.39" evidence="1"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553164.1">
        <property type="nucleotide sequence ID" value="XM_002553118.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEX1"/>
    <dbReference type="FunCoup" id="C5DEX1">
        <property type="interactions" value="58"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEX1"/>
    <dbReference type="GeneID" id="8295403"/>
    <dbReference type="KEGG" id="lth:KLTH0D10450g"/>
    <dbReference type="eggNOG" id="KOG2926">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_832050_0_0_1"/>
    <dbReference type="InParanoid" id="C5DEX1"/>
    <dbReference type="OMA" id="LRPIQEP"/>
    <dbReference type="OrthoDB" id="541883at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004314">
        <property type="term" value="F:[acyl-carrier-protein] S-malonyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.250">
        <property type="entry name" value="Malonyl-CoA ACP transacylase, ACP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.366.10">
        <property type="entry name" value="Malonyl-Coenzyme A Acyl Carrier Protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001227">
        <property type="entry name" value="Ac_transferase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014043">
        <property type="entry name" value="Acyl_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050858">
        <property type="entry name" value="Mal-CoA-ACP_Trans/PKS_FabD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42681">
        <property type="entry name" value="MALONYL-COA-ACYL CARRIER PROTEIN TRANSACYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42681:SF1">
        <property type="entry name" value="MALONYL-COA-ACYL CARRIER PROTEIN TRANSACYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00827">
        <property type="entry name" value="PKS_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Malonyl-CoA:ACP transacylase (MAT)" evidence="5">
        <location>
            <begin position="5"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR22726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="326" mass="36214" checksum="91ED3E6697219FA0" modified="2009-07-28" version="1">MKVITFPGQGTPVALNVFRSYLAEGSSAFRAVFRDSGRIGVTRTIEQRPADPVSIAACSFLLFDLYRRSRKNNAEELLFLGHSLGELGCLGAGNRLFGLSETMKIASLRNKLMINAAGGNKYELWAISAPRSQNLECELQPLLRVTDVNLANVNSRMQCVVTVPQGYTTDSKGTPQWAAELALQVPRCRIQRLENPYHIPFHNKNILQGIEEPLLDHMWQKLKSQGLHTKRELEHPIISNLNGKRITDVDKALESFAKSSCNVVNFVACCDTLNSLGVSKAVHIGPGEVIGKLMHRNCKLEKSQEWNTVEDITKELGDVQDSNSLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEY2</accession>
    <name>C5DEY2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GrpE protein homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0D10692g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the GrpE family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553175.1">
        <property type="nucleotide sequence ID" value="XM_002553129.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEY2"/>
    <dbReference type="FunCoup" id="C5DEY2">
        <property type="interactions" value="916"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEY2"/>
    <dbReference type="GeneID" id="8295414"/>
    <dbReference type="KEGG" id="lth:KLTH0D10692g"/>
    <dbReference type="eggNOG" id="KOG3003">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057217_0_0_1"/>
    <dbReference type="InParanoid" id="C5DEY2"/>
    <dbReference type="OMA" id="PHRHQAI"/>
    <dbReference type="OrthoDB" id="201635at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001405">
        <property type="term" value="C:PAM complex, Tim23 associated import motor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000774">
        <property type="term" value="F:adenyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00446">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.22.10:FF:000002">
        <property type="entry name" value="GrpE protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.20.20:FF:000011">
        <property type="entry name" value="GrpE protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.22.10">
        <property type="entry name" value="Head domain of nucleotide exchange factor GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01151">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000740">
        <property type="entry name" value="GrpE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013805">
        <property type="entry name" value="GrpE_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009012">
        <property type="entry name" value="GrpE_head"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21237">
        <property type="entry name" value="GRPE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21237:SF23">
        <property type="entry name" value="GRPE PROTEIN HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01025">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00773">
        <property type="entry name" value="GRPEPROTEIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58014">
        <property type="entry name" value="Coiled-coil domain of nucleotide exchange factor GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51064">
        <property type="entry name" value="Head domain of nucleotide exchange factor GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01071">
        <property type="entry name" value="GRPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4 6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="49"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="64"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR22737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="238" mass="26800" checksum="31AD297F5668C713" modified="2009-07-28" version="1">MRLLTSNIPKMVVRPTMGARLCTRMTARPATMTKFQTPTLGMRFYSDAAKQSEAAENAEGAQEESPKGPASEEQKKVQELEEKLAVKDKEAAEYKDRLLRSVADFRNLQEVTKKDIQKAKDYALQKFAKDLLDSVDNFGHALNAFKPESTQQSTELSELYTGVKMTKDIFEKTLKKHGIEKIDPMGETFDPNRHEATFELPNPEKQPGTVFHVQQVGFTLNNRVIRPAKVGIVKDPAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEZ4</accession>
    <name>C5DEZ4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Autophagy protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0D11000g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in cytoplasm to vacuole transport (Cvt) and autophagic vesicle formation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Conjugated with ATG12.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Preautophagosomal structure membrane</location>
            <topology evidence="1 7">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the ATG5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553187.1">
        <property type="nucleotide sequence ID" value="XM_002553141.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEZ4"/>
    <dbReference type="FunCoup" id="C5DEZ4">
        <property type="interactions" value="378"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEZ4"/>
    <dbReference type="GeneID" id="8295427"/>
    <dbReference type="KEGG" id="lth:KLTH0D11000g"/>
    <dbReference type="eggNOG" id="KOG2976">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051894_2_0_1"/>
    <dbReference type="InParanoid" id="C5DEZ4"/>
    <dbReference type="OMA" id="SIQKAVW"/>
    <dbReference type="OrthoDB" id="272162at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034274">
        <property type="term" value="C:Atg12-Atg5-Atg16 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044233">
        <property type="term" value="C:mitochondria-associated endoplasmic reticulum membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061908">
        <property type="term" value="C:phagophore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019776">
        <property type="term" value="F:Atg8-family ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.190">
        <property type="entry name" value="Autophagy protein Apg5, helix rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007239">
        <property type="entry name" value="Atg5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048940">
        <property type="entry name" value="ATG5_HBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042526">
        <property type="entry name" value="Atg5_HR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048939">
        <property type="entry name" value="ATG5_UblA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042527">
        <property type="entry name" value="Atg5_UblA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048318">
        <property type="entry name" value="ATG5_UblB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13040">
        <property type="entry name" value="AUTOPHAGY PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13040:SF2">
        <property type="entry name" value="AUTOPHAGY PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20637">
        <property type="entry name" value="ATG5_HBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20638">
        <property type="entry name" value="ATG5_UblA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04106">
        <property type="entry name" value="ATG5_UblB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0072">Autophagy</keyword>
    <keyword evidence="3 7" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <keyword evidence="4 7" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Autophagy protein ATG5 UblA" evidence="10">
        <location>
            <begin position="9"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Autophagy protein ATG5 alpha-helical bundle region" evidence="9">
        <location>
            <begin position="135"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="Autophagy protein ATG5 UblB" evidence="8">
        <location>
            <begin position="198"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF20637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF20638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR22749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="296" mass="34430" checksum="06836D46DAA44C2E" modified="2009-07-28" version="1">MDNVRRRAWAGSLNLRVTVHPDLTLNSSNEPEHIISFRVPREIYLVLYFPYILERVAPELRTNVTDFYNGWWLEMEGVPLSWNFPAGVLFDSLTGLDPALRSSRHRHNSLNVWELTLRHEDQCPTNVIPIVRGRDQVREFWMHQWKQACFILNGASKQVMSLSKPDTMKFWDSILRRDQNSFDTIKEKILPTFDNTRYVPLRVHLALPQIRFLEPQAKVQHNNGHIRLGDVLSSEFPEWFPNDSSTPNLAKAVIQGIEVPLQLSVWYLYQELSSFDGFLHVSLCLMTEDASLTSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DF02</accession>
    <name>C5DF02_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Serine/threonine-protein phosphatase 2A activator</fullName>
            <ecNumber evidence="6">5.2.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Phosphotyrosyl phosphatase activator</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0D11176g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the PTPA-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="6"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553195.1">
        <property type="nucleotide sequence ID" value="XM_002553149.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DF02"/>
    <dbReference type="FunCoup" id="C5DF02">
        <property type="interactions" value="568"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DF02"/>
    <dbReference type="GeneID" id="8295435"/>
    <dbReference type="KEGG" id="lth:KLTH0D11176g"/>
    <dbReference type="eggNOG" id="KOG2867">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030733_0_0_1"/>
    <dbReference type="InParanoid" id="C5DF02"/>
    <dbReference type="OMA" id="YLWGAAQ"/>
    <dbReference type="OrthoDB" id="16120at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008160">
        <property type="term" value="F:protein tyrosine phosphatase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04087">
        <property type="entry name" value="PTPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1150:FF:000002">
        <property type="entry name" value="Serine/threonine-protein phosphatase 2A activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004327">
        <property type="entry name" value="Phstyr_phstse_ac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043170">
        <property type="entry name" value="PTPA_C_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037218">
        <property type="entry name" value="PTPA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10012">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE 2A REGULATORY SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10012:SF5">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 2A ACTIVATOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03095">
        <property type="entry name" value="PTPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016325">
        <property type="entry name" value="Phstyr_phstse_ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140984">
        <property type="entry name" value="PTPA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0697">Rotamase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR22757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="353" mass="40246" checksum="2C9D86DE9098D750" modified="2009-07-28" version="1">MKPQKRLLTQDDMDIWSHSETFNNLISFLSGLAESVKGLENNEYQKPTGSSIESCCQLLEEVASLAEQYPVKQDAETSRFGKVEFRYFYDDLKSKSQGLIQRAFDALDGTQCEELSVYFAESWGDRSRIDYGSGHELNFLCFLYGLKEFGVFKEPADYRNMVLKLFIKYLGVMRTLETKYWLEPAGSHGVWGLDDYHFLPFLFGAFQLSTHKHLKPKSIHNSEVVEMFGKRYLYFGCIDFINKVKTTASLRWHSPMLDDISGVKTWAKVAEGMIKMYKAEVLNKLPIMQHFFFGSFLQCPEGISPPGTHAESHQHCSHSPLENTWGDCCGIRVPSAVAATESQKAGPKRIPFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>C5DF05</accession>
    <name>C5DF05_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Serine/threonine-protein kinase RAD53</fullName>
            <ecNumber evidence="7">2.7.12.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0D11242g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Controls S-phase checkpoint as well as G1 and G2 DNA damage checkpoints. Phosphorylates proteins on serine, threonine, and tyrosine. Prevents entry into anaphase and mitotic exit after DNA damage via regulation of the Polo kinase CDC5.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHEK2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.12.1" evidence="7"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553198.1">
        <property type="nucleotide sequence ID" value="XM_002553152.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DF05"/>
    <dbReference type="FunCoup" id="C5DF05">
        <property type="interactions" value="537"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DF05"/>
    <dbReference type="GeneID" id="8295438"/>
    <dbReference type="KEGG" id="lth:KLTH0D11242g"/>
    <dbReference type="eggNOG" id="KOG0615">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_379543_0_0_1"/>
    <dbReference type="InParanoid" id="C5DF05"/>
    <dbReference type="OMA" id="HEGPLKD"/>
    <dbReference type="OrthoDB" id="10252171at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004712">
        <property type="term" value="F:protein serine/threonine/tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009202">
        <property type="term" value="P:deoxyribonucleoside triphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000651">
        <property type="entry name" value="Serine/threonine-protein kinase RAD53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.20:FF:000104">
        <property type="entry name" value="Serine/threonine-protein kinase RAD53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000699">
        <property type="entry name" value="Serine/threonine-protein kinase RAD53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016256">
        <property type="entry name" value="Ser/Thr_kinase_Rad53"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000661">
        <property type="entry name" value="Ser/Thr_PK_RAD53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="FHA" evidence="13">
        <location>
            <begin position="66"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="188"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="FHA" evidence="13">
        <location>
            <begin position="619"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="493"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="551"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="758"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="499"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="551"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="758"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="309"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="194"/>
            <end position="202"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="329"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="11">
        <location>
            <position position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000661-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000661-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR22760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="795" mass="89135" checksum="CF926E5782EC4540" modified="2009-07-28" version="1">MDYVTQPTQQATQATQKYIIEKFSQEQIDDDIVCRMICTTGQIPIRDLRAELRDVVGKKESIKKLWTFGRNPQCDYHLGNTSRFSNRHFQIMLGEDGNLLLRDTSTNGTWLNGERLVKERNQILSQGDEITVGWGVSQDVLSLVVFINDKFKQRLEAAKAHGSKSAKSRSSRGASSSAQLTGILRDYSIKDEVVGQGAFATVKKAIERRTGKTHAVKIINKRKVMGNMDGVTRELEVLRRLNHPRIVSLKGFYEDKESYYLVMEFVSGGDLMDFVAAHGSVGEDAGREITRQVLEAVKYIHAMGISHRDLKPDNILIERDDPVLVKITDFGLAKIQDNGTFMKTFCGTLAYVAPEVISGKNSEEKEGNKYSSLVDMWSIGCLVYVILTGHLPFSGSTQQELYKQISAGSYHEGPLKDYRISDDARDFIESFLQVNPRDRMTAEQALKHPWIRTADSQSFIDSQVSLSQSMSQQKASDNVSDAQQQFINMRATKPKDEQPQSSPQEPQTQQPVFKIPGHRPVRYTQSCVPENSQPSSLKQVRTANFDFNYASQASDESQQPVQIPRGRVPPRLADPDNEDDVRLNADSMTAAGGRFITLRSTAESSVKGVIEIKQGVNPFFIGRSQDCSCKIEDNRLSRIHCFVLKKRHPIGDSIHESPAQGLEDLWYCHSGTNLSYLNDVKLTQGTKALLYEGDEIKIISDRANNFVISFVVEINDSTGLFNHGKPFPGHERLVEQQTPDELGLTRKLTDLKKAFASQNSSKRVRSISLSQDPNKKAKRARLDKADKGGANLQFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>C5DF47</accession>
    <name>C5DF47_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0D12166g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553240.1">
        <property type="nucleotide sequence ID" value="XM_002553194.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DF47"/>
    <dbReference type="FunCoup" id="C5DF47">
        <property type="interactions" value="335"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DF47"/>
    <dbReference type="GeneID" id="8295480"/>
    <dbReference type="KEGG" id="lth:KLTH0D12166g"/>
    <dbReference type="eggNOG" id="KOG0590">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_82_2_1"/>
    <dbReference type="InParanoid" id="C5DF47"/>
    <dbReference type="OMA" id="EYAKKCT"/>
    <dbReference type="OrthoDB" id="6513151at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343">
        <property type="entry name" value="SERINE/THREONINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343:SF137">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE HRK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="131"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="82"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="367"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="22"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="52"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="372"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="384"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="423"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="458"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="473"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="494"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR22802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="583" mass="65056" checksum="3CA7CF61B318A8D2" modified="2009-07-28" version="1">MPTLLGRNPFHHPKGQHPPPSLGSQADNVRSSRSFLGFMNHTSSRESTGSETFREPSDIPKRSGGSMNELKRFFRPAILHRKHSQSSLRNGSPEHHSHTQSAIPPSSDSSLSLSNRVNIYHDDAILAQKYGKLGKVLGSGAGGSVRVIVRPSDGATFAVKEFRPRRPTELVKDYAKKCTAEFCIGSTLHHPNVIETLDIFSDAKQNKYYEVMEYCPIDFFAVVMSGNMSRGEINCCLRQLTEGVKYLHSMGLAHRDLKLDNCVMTCDGILKLIDFGSAVVFKYPFDANVSMAHGIVGSDPYLAPEVMTSTKRYDPQFVDIWSIGIIYCCMMLKRFPWKTPKETDDNFRLFCLPDDVEHDYVKSAHEHETLMRQRRQEKHRARKLKEERVLAEKSEPSPVPNHGHGSEAAETQAAKGASAAPDNASQGASASAQKDTSPEAAPEKKNTKNEVVAETNEKPQSPQQHASDPQAPNEKDASNTEAPQEKPACDHKSGSRHHGKGRKVMHGPYRLLRLLPHASRPIMSRILTVDPKERATMEDMYNDEWFANIPFCTRDAKKGTIRAPGHTHTIVCEEDAHLESYKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>C5DF50</accession>
    <name>C5DF50_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 19">Mevalonate kinase</fullName>
            <shortName evidence="19">MK</shortName>
            <ecNumber evidence="3 19">2.7.1.36</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ordered locus">KLTH0D12232g</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Mevalonate kinase; part of the second module of ergosterol biosynthesis pathway that includes the middle steps of the pathway. The second module is carried out in the vacuole and involves the formation of farnesyl diphosphate, which is also an important intermediate in the biosynthesis of ubiquinone, dolichol, heme and prenylated proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>(R)-mevalonate + ATP = (R)-5-phosphomevalonate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17065"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:36464"/>
            <dbReference type="ChEBI" id="CHEBI:58146"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.36"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:17066"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="18 19">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 19">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 19">Belongs to the GHMP kinase family. Mevalonate kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.36" evidence="3 19"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553243.1">
        <property type="nucleotide sequence ID" value="XM_002553197.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DF50"/>
    <dbReference type="FunCoup" id="C5DF50">
        <property type="interactions" value="472"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DF50"/>
    <dbReference type="GeneID" id="8295483"/>
    <dbReference type="KEGG" id="lth:KLTH0D12232g"/>
    <dbReference type="eggNOG" id="KOG1511">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017814_0_1_1"/>
    <dbReference type="InParanoid" id="C5DF50"/>
    <dbReference type="OMA" id="LMDFNHG"/>
    <dbReference type="OrthoDB" id="1652964at2759"/>
    <dbReference type="UniPathway" id="UPA00057">
        <property type="reaction ID" value="UER00098"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004496">
        <property type="term" value="F:mevalonate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019287">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.890:FF:000003">
        <property type="entry name" value="Mevalonate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="entry name" value="GHMP kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006203">
        <property type="entry name" value="GHMP_knse_ATP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006205">
        <property type="entry name" value="Mev_gal_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00549">
        <property type="entry name" value="mevalon_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43290">
        <property type="entry name" value="MEVALONATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43290:SF2">
        <property type="entry name" value="MEVALONATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00959">
        <property type="entry name" value="MEVGALKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00627">
        <property type="entry name" value="GHMP_KINASES_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 19" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 19" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 19" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="14 19" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 19" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="16 19" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="13 19" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="15 19" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="6 19" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="20">
        <location>
            <begin position="118"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP kinase C-terminal" evidence="21">
        <location>
            <begin position="282"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00029310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00029438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU363087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="CAR22805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="415" mass="44151" checksum="BA9331C37AB41B68" modified="2009-07-28" version="1">MSLPLLTSAPGKVIIFGEHSAVFNEPAVAASVSALRTYLLVSAAQDPKVVELDFPDIKFDKQWSGEALATIAAGVQPEAVTDSRELNADLSNALESLLQPLSGSLHYQAAYCFLYMYFSLCGTRTGVKFTVKSSLPIGAGLGSSASLAVCLALALGKLGGHLKHGTESLTKEELAFVNKWSFIGEKCIHGTPSGIDNMVATYGSAVLFQRLPSGETKSEQILSFPQIPMVLTNTRIPRSTKVLVAGVKDLIVRQPEVMKPILESMGVVASSGAQILSQLTDANYESLCELVRVNHGLLVALGVSHPGLELIKSVGDSMNIGHTKLTGAGGGGCALTLLSRGARPEEVEKFKRTLESEHNYKTYTTGLGGPGCGYIAPELVQQNFSSIEPLFRKDSTASDLNKVLLPSSGSFQWIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFA9</accession>
    <name>C5DFA9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0D13618p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0D13618g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553302.1">
        <property type="nucleotide sequence ID" value="XM_002553256.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFA9"/>
    <dbReference type="FunCoup" id="C5DFA9">
        <property type="interactions" value="50"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFA9"/>
    <dbReference type="GeneID" id="8295544"/>
    <dbReference type="KEGG" id="lth:KLTH0D13618g"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035625_0_1_1"/>
    <dbReference type="InParanoid" id="C5DFA9"/>
    <dbReference type="OrthoDB" id="10018191at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050527">
        <property type="entry name" value="Snail/Krueppel_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24388:SF54">
        <property type="entry name" value="PROTEIN ESCARGOT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24388">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="215"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="105"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="249"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="116"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="270"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR22864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="414" mass="43004" checksum="FEA381DBC6F888D8" modified="2009-07-28" version="1">MNVSQSVTLPPISSFDSLVRAAEHEHFVRAQHGQRQRESALGAPGVGLSVGSAAHTPSAPSVASVGNVGSLGPVLVGAWSSGSDTTAAAATHNYFIASGSGAYGRADSRGEGGAGTRVVPGTTATAGTRRDAGPSFAGTLTRHESGGRASVSAPEPRPGVAPVSPDTNGSSGTAAGPAPRAAKPRRKKQCPVCHNFYANLSTHKSSHMLPEHKPHKCPVCARGFTRNNDLLRHRKRHWKDEIIGAGPGIGAAGAGATASGARGEDDALDVSPGASSSVSSGSARLGAADGADAADRAAGESLSPQQQLRSLHRIKGTFHCPYKSALIALDMDAYPGKRRELAFETSECHQTGVFSRCDTFKNHLKALHFEYPPGTKKKDRSSVPGRCKQCGRHFANVGEWLSSHVGKDCGYVYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFB0</accession>
    <name>C5DFB0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0D13640p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0D13640g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553303.1">
        <property type="nucleotide sequence ID" value="XM_002553257.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFB0"/>
    <dbReference type="STRING" id="559295.C5DFB0"/>
    <dbReference type="GeneID" id="8295545"/>
    <dbReference type="KEGG" id="lth:KLTH0D13640g"/>
    <dbReference type="eggNOG" id="ENOG502QQNB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033094_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFB0"/>
    <dbReference type="OMA" id="FYLFHNY"/>
    <dbReference type="OrthoDB" id="1461976at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03507">
        <property type="entry name" value="Delta12-FADS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012171">
        <property type="entry name" value="Fatty_acid_desaturase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32100">
        <property type="entry name" value="OMEGA-6 FATTY ACID DESATURASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="101"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="134"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="252"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="279"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid desaturase" evidence="3">
        <location>
            <begin position="104"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR22865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="418" mass="47357" checksum="1189357D23D248F8" modified="2009-07-28" version="1">MANIASSSGFERMQGGHGGGSSTQTETEKPKVAIDTHGNVFKVPDYTMKDVLSAIPAECYKRDLGKSLSYVARDIAVLGGLAYLANCVIGPALPEAWPVRFVFWSAYAAAMGLWSTGLWVLAHECGHQAFSESGVINDFVGWTLHSYLLVPYFSWKYSHSKHHKATGHLTRDMVFLPPTKEEFWEKRGWAANLAEYTEDSPLRTLGELVLQQLGGWLGHLFTNVTGQTYPGVPAWKRNHFWPTSPLFNSKDALYILLSDIGVVTQLVVLRMWYLKFGGWSLFINWFVPYLWVNHWLVFITFLQHTDQTLPHYDQSEWTFAKGAAATIDRQLWFVGPHIFHDIIETHVLHHFVSRIPFYNARPASAAIKKVMGEHYRSCDENMFKSFWKVARACQYVEGADGVLMFRNVNNEGVGMGPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFI2</accession>
    <name>C5DFI2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0D15290p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0D15290g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Mo25 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553375.1">
        <property type="nucleotide sequence ID" value="XM_002553329.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFI2"/>
    <dbReference type="FunCoup" id="C5DFI2">
        <property type="interactions" value="406"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFI2"/>
    <dbReference type="GeneID" id="8295619"/>
    <dbReference type="KEGG" id="lth:KLTH0D15290g"/>
    <dbReference type="eggNOG" id="KOG1566">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035755_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFI2"/>
    <dbReference type="OMA" id="AYDHKES"/>
    <dbReference type="OrthoDB" id="609103at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043539">
        <property type="term" value="F:protein serine/threonine kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013878">
        <property type="entry name" value="Mo25"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10182">
        <property type="entry name" value="CALCIUM-BINDING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10182:SF3">
        <property type="entry name" value="PROTEIN MO25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08569">
        <property type="entry name" value="Mo25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="364" mass="41929" checksum="59626F77922C9875" modified="2009-07-28" version="1">MAFWWKKTPKTASDYARLLSEQLNKFDNASASDSRRKVQDECSKYLSGIKHFLLGDVEPEPSCESVDELYDAIYQLDILHDLLAKMPELEFEPRKDVALIFATCLRRSRDNKFVTVDYLVTKPQTLSLMLRTPELALTKPNASDLFLKSGSIILECLKYEQLCRMMLRDPQLWVFFDLARGGSFEVSTESLQILTSFFTVHHKLVSTEFFSHQANLQKLIDKINKLMAHGNYVTKRQSVKLLHSLIMIRSYNQFLTTYINSPENLKLIMILLSDRSKNLQMGSFNIFKVFVANPRKSKPVSDIMVKNRDKLLHYLASFNADSKDTTFLDEKEYVVQEIESLPRLVSPNSDLTLGTSPQNRSTIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFI3</accession>
    <name>C5DFI3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0D15334p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0D15334g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553376.1">
        <property type="nucleotide sequence ID" value="XM_002553330.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DFI3">
        <property type="interactions" value="1018"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFI3"/>
    <dbReference type="GeneID" id="8295621"/>
    <dbReference type="KEGG" id="lth:KLTH0D15334g"/>
    <dbReference type="eggNOG" id="KOG0920">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001832_4_0_1"/>
    <dbReference type="InParanoid" id="C5DFI3"/>
    <dbReference type="OMA" id="KVEYEMK"/>
    <dbReference type="OrthoDB" id="5600252at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17917">
        <property type="entry name" value="DEXHc_RHA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00048">
        <property type="entry name" value="DSRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23827">
        <property type="entry name" value="RWD_YLR419W-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14271">
        <property type="entry name" value="UBA_YLR419W_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056328">
        <property type="entry name" value="DSRM_DHX29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059023">
        <property type="entry name" value="RNA_hel_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006575">
        <property type="entry name" value="RWD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035467">
        <property type="entry name" value="YLR419W-like_UBA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF267">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE YLR419W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24385">
        <property type="entry name" value="DSRM_DHX29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26026">
        <property type="entry name" value="RNA_hel_CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05773">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00591">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50908">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RWD" evidence="6">
        <location>
            <begin position="417"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="602"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="8">
        <location>
            <begin position="834"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="530"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="28"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR22938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1420" mass="159636" checksum="3AEEAB06D7702561" modified="2009-07-28" version="1">MAKKPSKTPPAPVAVEPVTKNKRGKKGARVETEEEKKARLANRAKVTSTSSWTGKLPHTLLHENCQKRKWNKVEYDMKKIGDTGMLAIAVLSFTDPKTKETLTVRMNDPTYDKKSGKGILTPQETPMEARHMAATVALCRIASNSNMHMTLPPNHRKLWYDLVDFRKKLLKENPGLAPRLFDPEPFKTLVADRKHKEVKEKERATKQQQAEKIQKAPTVITSVSAKSAPAAKNIVLKKRNAGPFDKRIIRFPQKVWDNATFVDFKESSRNLIELSLKSHIDWSSKKYEGNDDRQLLKNKLVSLGFREPHVKEALLYKDPLSFLLFNLPEDDLPSFFQKRQEDTKMRIEIASLPLKTQNMVSRLMESGVSYAEALYALECNNFDESEAAGKLTAKLSAQPQEPVSEISPEESMDIWTQELESLQSIYTQSIEVLNEEKTCYTIKLIDEFSLKLKVYKTKNYPCSLPGLVVSTFDKKLKLPNYIKQIILTKLIDYISESGLLGDMLIYHAYEWLQENIAGIIDNPGSLLPESTANRHGVDRDGATRTGKGKSRKGKGAVSRPLTSNEIEKLKSDYRERTCSKRFASMMKQRSQLPAWKIQDAIVDLIRNNDVVLITGETGSGKSTQVVQFVLDYLMSKEDDFGNTKIICTQPRRISAIGLAERVSEERCTDCGTEVGYMIRGVNKTKPSTRIKFMTTGVLVRILQSSRDFLANTIVFIDEVHERSIDTDLIVILLKNLKGKVPGLKIVLMSATVNVDIFKKYFGQLGSCHIEGRTFPIQDHYLEEILETLDFKIKRRNNGYNDFQDDDEMNENGSNELKPGADSNFFRSGQINYDLISSLTVHIHEKLNLEGNDGSIVIFLPGVGEINTCCRNIKSSRESQNFVVLPLHSALTPDAQKSVFKRFPGKRKIVVSTNIAETSITIDDCVATIDSGRVKTMFYDAKTNTTRLVETFVSKAEAKQRRGRAGRVRAGHSYKLFSKTRYEEMPAQPTAEIKRVPLESLYLSVKSMGIKDVVAFLGSGLDPPPLDALKKSEQLLTTSGLLSEEDNLLTELGKFIGLMPVMDHKHGKLLIYSIIFGCTDFGVNTVAVLSIGGSPFIVTQENRDEIKKICQQYSSSGDLLAYVEVVRRYLSLEDGASRRKFMGENHLSFNKMKEITSSRAQLLSILKDIGFVPMSYSMGSSDYLNRNGNNTEIIKCILTGAFYPQVARIQHPDQKFINTSAGAIEKDPEARMIKYWIRNEEYINQLYGLAKDSEVTNTSLPATQAFLHPSSIFFSTKNDDVSELAGLHEMLSSEKPEKGGSGQNVLKASFAVFNSSHSTNKLYLRDITPTSTLALLLFGGAIKYDLQGTEHSPGIVVDNWLPVRTWCKNGVLLKELRTLLDNVIKQKLEQPEYNTSQESTHSGDDVLTIVEEIVKTESSNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFQ1</accession>
    <name>C5DFQ1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0D16962p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0D16962g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR23006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553444.1">
        <property type="nucleotide sequence ID" value="XM_002553398.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFQ1"/>
    <dbReference type="STRING" id="559295.C5DFQ1"/>
    <dbReference type="GeneID" id="8295693"/>
    <dbReference type="KEGG" id="lth:KLTH0D16962g"/>
    <dbReference type="eggNOG" id="KOG0254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_22_1_1"/>
    <dbReference type="InParanoid" id="C5DFQ1"/>
    <dbReference type="OMA" id="KLSHMDP"/>
    <dbReference type="OrthoDB" id="10021397at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17502">
        <property type="entry name" value="MFS_Azr1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1720.10">
        <property type="entry name" value="Multidrug resistance protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF198">
        <property type="entry name" value="AZOLE RESISTANCE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="75"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="132"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="229"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="258"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="300"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="341"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="369"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="403"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="446"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="512"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="9"/>
            <end position="536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR23006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="571" mass="61381" checksum="476C73DCCC803E05" modified="2009-07-28" version="1">MRKVAVIVCVTSLCVTLFLGALDIVICITLYDTIGQEFNDYANVGWLMAGYNLPNALFMLLWGRIASLVGLKCSLMASIVIFEAGSLMAGAANSMGMLIGARVISGFGGSGIESLVYGVGTSIVEEKHRARVITVLGIAYMLAEGAGPFVGGGLAEHVSWRWCFYVNLPIGGVALAALGWGYNPAGRAPGRALARFWVRARAFPYGTLLQAQFWRRAAALMVFRLDIPGLALSSGGFALVMLALTFGGNQYAWRSATIVCMFVFGAALLALFMLYDFVVLPRVGARIAGYEPLPLVPWRLAANVPVLTSSLCGFFNCFAFELQSIYLVQYYQLVVNKGPTLASVHLWQMSLPALVSVIASAVVNERFGVVKPLVLAGVVMGFVGSGLLTLVRSDTTLGQSIGFSVLAGASFGCVYQLSLISAQLQVERDDPDFHARFIEVTSYNTFIKTLGFSFAGIVSTTVFTVSLKNLASSHSASVPSFSTVEDLIRYRSENFDGRNSLLGRLLAKSVRNVFYCALVCSALSFLFGLFTSKKRTDITKGAESTELAELCELAEPAEEKPVSKTPTSTLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFS2</accession>
    <name>C5DFS2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Vacuolar membrane-associated protein IML1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Vacuolar membrane-associated protein iml1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0D17468g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IML1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR23027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553465.1">
        <property type="nucleotide sequence ID" value="XM_002553419.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DFS2">
        <property type="interactions" value="741"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFS2"/>
    <dbReference type="GeneID" id="8295715"/>
    <dbReference type="KEGG" id="lth:KLTH0D17468g"/>
    <dbReference type="eggNOG" id="KOG3572">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000935_1_1_1"/>
    <dbReference type="InParanoid" id="C5DFS2"/>
    <dbReference type="OMA" id="SWMNATP"/>
    <dbReference type="OrthoDB" id="39497at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990130">
        <property type="term" value="C:GATOR1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904262">
        <property type="term" value="P:negative regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010508">
        <property type="term" value="P:positive regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04449">
        <property type="entry name" value="DEP_DEPDC5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000591">
        <property type="entry name" value="DEP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045838">
        <property type="entry name" value="DEPDC5_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027244">
        <property type="entry name" value="IML1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048255">
        <property type="entry name" value="IML1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13179">
        <property type="entry name" value="DEP DOMAIN CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13179:SF8">
        <property type="entry name" value="GATOR COMPLEX PROTEIN DEPDC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00610">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19418">
        <property type="entry name" value="DEPDC5_CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12257">
        <property type="entry name" value="IML1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00049">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50186">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEP" evidence="6">
        <location>
            <begin position="1192"/>
            <end position="1267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="116"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="739"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="741"/>
            <end position="752"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR23027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1575" mass="179773" checksum="108FEB944D84ECB5" modified="2009-07-28" version="1">MRRRSNASELKLCFNHVSLNASADTNDCKAPYMNNDELSVSNTQPERRNFPESLQGFLIKRSDQNSQSARASNRATNSLKNMQIDSNTITIGGSTTPKRPGKYRLRMDLNVHNVGKVLPNQDSSAGTAIASGHSELSRSPSALPRRETATKKKKAFRLELGLHEFRFSEEQVLLDLSCVTGIKEGDLAELKTYQGKNGSKGKKIYFIVKAFDRDLRKRYKNAQISILSGQLQHTLGLPSRSKVWVKVKDKESTQADVVEINVKDCLIHRGDMWVFSSEITGGCVYCEQKLTFVDSVRATVKAIYREGKKVLSGYIGNQTKVVFRSESARLIFLIQITDEMWHFEENGDIYFYKVVNSVFPTIFKKWKNIGTHHTITIVFSASLDFSDVAFKDLPEGERPKNTKDYYRIVVDQVNIVHWTEIMKTLRREFKNIAKDLKTFKTEDGTSVLKGRFSPVIKSNILETMCLAATFVTNPFRQPDLRRTVAHVIIVSPGTGIYDVDYDLLKATSTKIQSLELSIDVLCLARPPLHIVPLLRFRDYENKLRYCAPSWINISFWRHSSNSSVWYPRCKMHDLQMMGLTETEMKEQVEIDYMDAPSAAESVMAMMKNHDEDIFSSQVSTDNVQDRYSVSSAPSNGKKATVKKSFKEISGKAPLAWNPPKSASPMVQSTASLQVFGDLAQSASTGLLDDKETKSSFTKLDKGSQKDSRAINTLKNATKSSNIAQRLVSKFLPEIESKNKKTSTNLPYNSSDSQSRHLALGNSPDIDDNGVHQQWPVLKKNLSSFERPDRKKTTFDVSSFCSQSFVGSPNRFTDSPRRSIDSPRPKYVAKELPQDMSATLFSWLEIKNVSRPISSERAGHLIAPQWKDVFPRYVAKKYSKWRSLTTPAELPITISSFPAKEDFEKNFTFTNHSVISNPDQEVGNQTAFDLLRDMIYVRLLAGFQICVGDSLERLESSKNNEGDTRRITKILTKHNYMDVKFFMMLGEEIHRLTCTLDGTIDVQRHIRKKLSESSFGVPTYCPEIKTRYENQYREIMTDPLKMNREKVNWNQLDQMLAGYEDSIIEQNKKRFRSKFVILPADVPENTISSTINGRSETLSAEEIRLEGFRRLISSIYRARLRNAQDEVSRSSRKEEIAPEVIFYTGSLFTFIDEQAEILRSSGKVKHDTIFIRDEDKLSKKVSLSKLAQEMQHGKNSLNLVNRKWHWKRHNNCFIGSEFVNWLIENFLDIDTRDEAVIYGQDLMYQGLFVHVENRHGFLDGHYFYHFSPDYVHSSEVRDPPQDQRSLYTDENVRRVSAPDGADSKLSRIGTGDAVVGDEGSMSNSKLSTHKPTVLLSNMITVNADSAGKSYKPEVCRVHYDRVHNPDHCFHIRLEWLTATPKLIDDLINSWARLCKRYGLNLVEVPWVELCTIPLSNPFHTFVDITLAINPWEDPEFKDEQLISQSPYYYHTFLLEKSGFLIDNRASQLLNQSVTDHEVMYSWGKPKFKYAQYIHLTGAYIAEIRENGNLFLAPNNRHLSRVNVDSVTLKVKQSPTFALDAQKIMLAFKGTCLDYEKLRSIFLDAKGVWQSDKSKPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFY3</accession>
    <name>C5DFY3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0D00902p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0D00902g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The proteasome degrades poly-ubiquitinated proteins in the cytoplasm and in the nucleus. It is essential for the regulated turnover of proteins and for the removal of misfolded proteins. The proteasome is a multicatalytic proteinase complex that is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. It has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase T1A family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552763.1">
        <property type="nucleotide sequence ID" value="XM_002552717.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFY3"/>
    <dbReference type="FunCoup" id="C5DFY3">
        <property type="interactions" value="1344"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFY3"/>
    <dbReference type="GeneID" id="8294983"/>
    <dbReference type="KEGG" id="lth:KLTH0D00902g"/>
    <dbReference type="eggNOG" id="KOG0184">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035750_0_1_1"/>
    <dbReference type="InParanoid" id="C5DFY3"/>
    <dbReference type="OMA" id="RVSMYMH"/>
    <dbReference type="OrthoDB" id="40134at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010499">
        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03751">
        <property type="entry name" value="proteasome_alpha_type_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000044">
        <property type="entry name" value="Probable proteasome subunit alpha type-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0647">Proteasome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="5">
        <location>
            <begin position="8"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="241"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="247"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="262"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR22325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="277" mass="30151" checksum="84DE41E188DB620D" modified="2009-07-28" version="1">MTSIGTGYDLSNSVFSPDGRNFQVEYAVKAAENGATSVGMKFKDGIVLAVEKLVTSKLLVPGKNKKIQTIDRHVGCAYSGLMPDGRHLVNRGREEASSFKKTYATPIPLPALADRLGQYVQAHTLYNSVRPFGITAIVGGVDDDGAHLYMLEPSGTYWGYKGAATGKGRQSAKAELEKVISNKPELDAREAVKEAARIIYLAHEDNKEKDFELEMSWCSISETNGLHKAVPKDLLDEAIAFAKAENGDDSDSDDSSDESENEKDNEKKDAEGDVQLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>C5DG16</accession>
    <name>C5DG16_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0D01650p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0D01650g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552796.1">
        <property type="nucleotide sequence ID" value="XM_002552750.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DG16"/>
    <dbReference type="GeneID" id="8295016"/>
    <dbReference type="KEGG" id="lth:KLTH0D01650g"/>
    <dbReference type="HOGENOM" id="CLU_2886222_0_0_1"/>
    <dbReference type="InParanoid" id="C5DG16"/>
    <dbReference type="OMA" id="ADKHNKN"/>
    <dbReference type="OrthoDB" id="4030347at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="63" mass="6242" checksum="1849F22962489D48" modified="2009-07-28" version="1">MPEKNAAAHDQGNLAGSIAGSVAGADLGNKLHLGAIGGIASSVLGSGQGSKLENKLKEKMDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>C5DG42</accession>
    <name>C5DG42_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0D02244p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0D02244g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552822.1">
        <property type="nucleotide sequence ID" value="XM_002552776.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DG42"/>
    <dbReference type="FunCoup" id="C5DG42">
        <property type="interactions" value="34"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DG42"/>
    <dbReference type="GeneID" id="8295042"/>
    <dbReference type="KEGG" id="lth:KLTH0D02244g"/>
    <dbReference type="eggNOG" id="ENOG502QS9U">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012509_0_0_1"/>
    <dbReference type="InParanoid" id="C5DG42"/>
    <dbReference type="OMA" id="LRMQFKL"/>
    <dbReference type="OrthoDB" id="3832628at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070086">
        <property type="term" value="P:ubiquitin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050357">
        <property type="entry name" value="Arrestin_domain-protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024391">
        <property type="entry name" value="LDB19_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188">
        <property type="entry name" value="ARRESTIN DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188:SF76">
        <property type="entry name" value="PROTEIN LDB19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13002">
        <property type="entry name" value="LDB19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LDB19 N-terminal" evidence="2">
        <location>
            <begin position="136"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="328"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="372"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="574"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="648"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="328"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="380"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="592"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="649"/>
            <end position="662"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR22384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="752" mass="82654" checksum="7844FB2B2179EA99" modified="2009-07-28" version="1">MVFSKLGHQSDKKSASNGALDRVFSDSSNDGLVELLIDIESPPCVLYGSATESTGALLSGLLKLRVRSRSDHISHSEPLTPTTSHGRKKKSSSGLNALSQTLSNLSLSNSAMSPVTSPKPSRTSSMMFTKVSVKSVSLSLVQKVRYTKPFVAHSSSIQSCLNCRMKQTELARWDIVKKETEIPVGDHAYPFSHLIPGSVPATVCLGSEAHTEVKYELVGEATYRAVPSHNSRGEKTSTVHLKLPIPVTRSILRGPDKNSLRIFPPTDLTATVVLPNIVYPKSTFTLELKLDGVSSEGRRWRMRRLNWKIEEKGRVRLQACATHKGKLKQLEKEVKETETGKKPSKGKPLKRTHDAAPQVTTTVCTLEDALSMTNAPEDPNAPDETETQEETSNRNQGEDDGLIHPSDSAMRQARVEEQQRLRQEMLQKELSEGTALFTEELRTIAHGEVKNGWKSDFTGSGKIELVTEVNCMGLNSGVSNPVNRASSSKPVHDNGRQHVTVACDVEDPHLGIYVQHLLILEIIVAEEALQYANGQPLHTEHSPLTKTSSNATNPDQRLAELSPMLAARSSVPQNINDEAHPNQPEHGAPSPHSASRRNSNSRIVGVPTGAARVLRMQFRLNITERSGLGISWDDEVPPTYQDVRLLSPPSYNNATSGSNTPLVHSDMDRNDYSTRTNFNSGRSEFTELSRPPQAHAYDSPYQSLSPVQSPQLENVVSIQGNAHHTHLLTPQNTQEVHLPSLSELLDTDRITQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>C5DG68</accession>
    <name>C5DG68_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Structural maintenance of chromosomes protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0D02816g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SMC family. SMC5 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552848.1">
        <property type="nucleotide sequence ID" value="XM_002552802.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DG68"/>
    <dbReference type="FunCoup" id="C5DG68">
        <property type="interactions" value="1077"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DG68"/>
    <dbReference type="GeneID" id="8295068"/>
    <dbReference type="KEGG" id="lth:KLTH0D02816g"/>
    <dbReference type="eggNOG" id="KOG0979">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004969_2_0_1"/>
    <dbReference type="InParanoid" id="C5DG68"/>
    <dbReference type="OMA" id="RFWTSQP"/>
    <dbReference type="OrthoDB" id="10254973at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030915">
        <property type="term" value="C:Smc5-Smc6 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22879">
        <property type="entry name" value="Smc5_CC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001301">
        <property type="entry name" value="Structural maintenance of chromosomes 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038729">
        <property type="entry name" value="Rad50/SbcC_AAA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45916">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45916:SF1">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13476">
        <property type="entry name" value="AAA_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad50/SbcC-type AAA" evidence="12">
        <location>
            <begin position="50"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="278"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="401"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="672"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF13476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR22410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1094" mass="125765" checksum="39180B521634AF34" modified="2009-07-28" version="1">MLSSGEERHKYDPNALPRTHNEEDGAPSSKRLKLGEDDLALFRAGSIVTLRLENFVTYALTEFHMSPSLNMIIGPNGSGKSTFVCAVCLGLAGKPEYIGRSTKIEDYIKNGEDRSVIEVTLKRDPEAEDRYVASDGTTKVTRVLHRNRKASEYFLNGQSVTESAVKRLVSELNIQLDNLCQFLSQERVEEFARLKSDKLLVETARSVDVNMVADLEDLKALQGEELVEAKELELKSSRVLELKATRDKLTASVRALENFKKKKEELGMHEKLLPYVQVKDHKVRIKQYKREYEKMRKQLKDLISDKKPFRHTQQLIETETVERNENKKKLEVQLKVSKRTFPSYLEKLEHLKEKVRGKQAQLKYYEDRTGKIKDEIVVVEHNLKEEQAKREALPVPDQGAFERIQAERKEITDKGVELEQQRTDLNANLVNADYHMDILKRKLQQENESLVSTDRIGILDGKRFEELRNAVLFMRSRPEMQGKVLEPPIMSISVKDPQFASYLGTCTDFATSLALTVVDSDSYRTFGDELLNKFRVNIRELSAAPTTSPLPLSELRKLGFQGYLSDFLSGDNGVLKMLCQQHKLHTIPVSRTAMSSRLMDYLMKPDESNNLKFRRVIAGNYVYDFKRSAYGRRQIFSSEFKVRPCQFFKGSVLTEERKGEIKESMARINGQIQAKKDEVQFITQKIQELRSELQSNKQEDAELHKITSKLNDQKKTIAHCSHRIKALENKLSMLKTDLQQDVTTKINDCRESIKSSLREQVKVMSGITQFVAKLQGLQREISLATIEHLEAFNTERSMNEVIGFFNEKEEQLRGAYEEAKNKYASMKETDEYRGWMDKIRHYTDAEKTQLADLAEKYQQSNNFDLSFISNTIDRLRSEIGMLNQDESVLEIMRQTEAELGELDCVVPRKEAALLEIRRTIQEKRSQLEPKLDAIVEKISQNFCALFKNVGSAGAVRLDKPEQFSDWKIEIMVKFRDNATLKRLDSHTQSGGERAVSTVLYMIALQDVTSAPFRVVDEINQGMDSRNERIVHKSMVESACSQNNSQYILVTPKLLTQLHYHEKVRIHCVMAGAWIPDPIVDHDKIRFGQTSNYVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DG84</accession>
    <name>C5DG84_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0D03168p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0D03168g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552864.1">
        <property type="nucleotide sequence ID" value="XM_002552818.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DG84"/>
    <dbReference type="FunCoup" id="C5DG84">
        <property type="interactions" value="125"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DG84"/>
    <dbReference type="GeneID" id="8295084"/>
    <dbReference type="KEGG" id="lth:KLTH0D03168g"/>
    <dbReference type="eggNOG" id="KOG2845">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042447_0_0_1"/>
    <dbReference type="InParanoid" id="C5DG84"/>
    <dbReference type="OMA" id="MWASPQE"/>
    <dbReference type="OrthoDB" id="338816at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0180022">
        <property type="term" value="C:RQC-trigger complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039128">
        <property type="entry name" value="TRIP4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009349">
        <property type="entry name" value="TRIP4/RQT4_C2HC5_Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12963:SF4">
        <property type="entry name" value="ACTIVATING SIGNAL COINTEGRATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12963">
        <property type="entry name" value="THYROID RECEPTOR INTERACTING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06221">
        <property type="entry name" value="zf-C2HC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TRIP4/RQT4 C2HC5-type zinc finger" evidence="2">
        <location>
            <begin position="177"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="447"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="105"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="118"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="447"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="483"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR22426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="521" mass="58764" checksum="AE728CCED726857F" modified="2009-07-28" version="1">MGKSSAISFAVREIPHIIPIDEESARQLCEQILSEYGEDPQAIAEKFMDILGPEDASLNFVLQFTEKLSHEESEKASKSALSPKPSTKNDTFASRSGRQGVNKPESAELRKSEEPSGVPAAPKANAPRKAVVRPSTRPNPKESSKKASKTRKVQSLTEIDDILKALELEQTESDPTKYACNCQGTRHPLFEVAPNCLSCGKIICLREGLNLNHCTFCHTEFLSAEERAQIIEVLNQEKQDLESSKPVAALEKPKKPKGYKITSGAGTNLFSEQDKLFDRVEKERQRELKRAEVLEGIDKEQSKAMHSQNGEDNADPELKVAQERLEKLLHFQDTSAQRTKIIDNASDFSMSNDGSVWGSAQDRALLLKKQQRNMRRWEKLERERHGGRDKVVMDLSIGKDGKVTMTEARRAPKTAHAFDEENVEDISDEEDLQDLQEITELKSTLMDEKKRIDKDLESNVWDPEKDKKQFQKPVYVNNESEISPEKGKPEKDGTRDRGWKNRVQISQGDENSLEQNILAVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGA3</accession>
    <name>C5DGA3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Aconitate hydratase, mitochondrial</fullName>
            <shortName evidence="14">Aconitase</shortName>
            <ecNumber evidence="14">4.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">KLTH0D03630g</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>citrate = D-threo-isocitrate</text>
            <dbReference type="Rhea" id="RHEA:10336"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:16947"/>
            <dbReference type="EC" id="4.2.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="14">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the aconitase/IPM isomerase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.-" evidence="14"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552883.1">
        <property type="nucleotide sequence ID" value="XM_002552837.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGA3"/>
    <dbReference type="FunCoup" id="C5DGA3">
        <property type="interactions" value="992"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGA3"/>
    <dbReference type="GeneID" id="8295105"/>
    <dbReference type="KEGG" id="lth:KLTH0D03630g"/>
    <dbReference type="eggNOG" id="KOG0453">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006714_2_2_1"/>
    <dbReference type="InParanoid" id="C5DGA3"/>
    <dbReference type="OMA" id="KKQGMLG"/>
    <dbReference type="OrthoDB" id="2224430at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003994">
        <property type="term" value="F:aconitate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01584">
        <property type="entry name" value="AcnA_Mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.19.10:FF:000002">
        <property type="entry name" value="Aconitate hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.499.10:FF:000003">
        <property type="entry name" value="Aconitate hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.499.10:FF:000004">
        <property type="entry name" value="Aconitate hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1060.10:FF:000001">
        <property type="entry name" value="Aconitate hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1060.10">
        <property type="entry name" value="Aconitase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="entry name" value="Aconitase, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="entry name" value="Aconitase, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050926">
        <property type="entry name" value="Aconitase/IPM_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015932">
        <property type="entry name" value="Aconitase_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006248">
        <property type="entry name" value="Aconitase_mito-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01340">
        <property type="entry name" value="aconitase_mito"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005558">
        <property type="entry name" value="PRK07229.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43160">
        <property type="entry name" value="ACONITATE HYDRATASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43160:SF3">
        <property type="entry name" value="ACONITATE HYDRATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="Aconitase iron-sulfur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52016">
        <property type="entry name" value="LeuD/IlvD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01244">
        <property type="entry name" value="ACONITASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="9 14" id="KW-0408">Iron</keyword>
    <keyword evidence="10 14" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="12 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 14" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha" evidence="15">
        <location>
            <begin position="63"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="Aconitase A/isopropylmalate dehydratase small subunit swivel" evidence="16">
        <location>
            <begin position="581"/>
            <end position="707"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU362107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAR22445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="779" mass="84900" checksum="B54C882E1EB70051" modified="2009-07-28" version="1">MLSARSIAQKPVARGLATVSKLTRDSKVHQNLLEDHSFINYKQNLENVDIVRKRLNRPLTYAEKILYGHLDDPHGQDIERGVSYLKLRPDRVACQDATAQMAILQFMSAGLPQVAKPVTVHCDHLIQAQVGGEKDLKRAIDLNKEVFDFLATATAKYNMGFWKPGSGIIHQIVLENYAFPGALIIGTDSHTPNAGGLGQLAIGVGGADAVDVMSDLPWELKAPKILGVKLTGKMSGWTSPKDIILKLAGITTVKGGTGKIVEYYGEGVNTFSATGMGTICNMGAEIGATTSVFPFNHSMVDYLKATRRGEIADFAKLYNSDLLSADKGAEYDEVIEINLSELEPYVNGPFTPDLATPISKMKEVAVHNNWPLEVKVGLIGSCTNSSYEDMSRAASIAEDAASHGLKSKSLFTISPGSEQVRSTIARDGQLKTFQDFGGVVLANACGPCIGQWDRQDIKKGDKNTIVSSFNRNFTSRNDGNPETHSFVASPEITTALAIAGDLRFNPMTDKLKDKDGNEFMLKPPTGVGLPVKGYDQGVNTYQAPPAERSSVEVKVSPTSDRLQLLTPFDAWDGKDPVDLPILIKSLGKTTTDHISMAGPWLKYRGHLENISNNYMIGAINAENKKANCVKNFFTGEYAGVPDTAREYKKRGQKWVVVGGENFGEGSSREHAALEPRYLGAYAIITKSFARIHETNLKKQGLLPLTFGKPEDYDRINPDDLVDLVGVTELAPGKPVTMRLKTKSGETWETPLNHTFNSEQIGWFKAGSALNMITQSKKSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGC1</accession>
    <name>C5DGC1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 11">Cystathionine beta-synthase</fullName>
            <ecNumber evidence="4 11">4.2.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0D04092g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 11">
            <text>L-homocysteine + L-serine = L,L-cystathionine + H2O</text>
            <dbReference type="Rhea" id="RHEA:10112"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:58161"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
            <dbReference type="EC" id="4.2.1.22"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-homocysteine and L-serine: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the cysteine synthase/cystathionine beta-synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.22" evidence="4 11"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552901.1">
        <property type="nucleotide sequence ID" value="XM_002552855.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGC1"/>
    <dbReference type="FunCoup" id="C5DGC1">
        <property type="interactions" value="1006"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGC1"/>
    <dbReference type="GeneID" id="8295126"/>
    <dbReference type="KEGG" id="lth:KLTH0D04092g"/>
    <dbReference type="eggNOG" id="KOG1252">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021018_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGC1"/>
    <dbReference type="OMA" id="KFADDEW"/>
    <dbReference type="OrthoDB" id="728at2759"/>
    <dbReference type="UniPathway" id="UPA00136">
        <property type="reaction ID" value="UER00201"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004122">
        <property type="term" value="F:cystathionine beta-synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019343">
        <property type="term" value="P:cysteine biosynthetic process via cystathionine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01561">
        <property type="entry name" value="CBS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.580.10:FF:000052">
        <property type="entry name" value="Cystathionine beta-synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000003">
        <property type="entry name" value="Cystathionine beta-synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000118">
        <property type="entry name" value="Related to CYS4-cystathionine beta-synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050214">
        <property type="entry name" value="Cys_Synth/Cystath_Beta-Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005857">
        <property type="entry name" value="Cysta_beta_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001216">
        <property type="entry name" value="P-phosphate_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01137">
        <property type="entry name" value="cysta_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10314">
        <property type="entry name" value="CYSTATHIONINE BETA-SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00901">
        <property type="entry name" value="CYS_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 10 11" id="KW-0129">CBS domain</keyword>
    <keyword evidence="11" id="KW-0198">Cysteine biosynthesis</keyword>
    <keyword evidence="7 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 11" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="12">
        <location>
            <begin position="361"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00026192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR22463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="493" mass="54116" checksum="4FCE60785572E14A" modified="2009-07-28" version="1">MTQYDSRTSVADLVGNTPMVHLTKLPKALGVKPQVYAKLELYNPGGSIKDRIAKSMIEDAEKSGRIHPSRTTLIEPTSGNTGIGLALMGAVKGYRTIITLPEKMSNEKVSVLKALGAEIIRTPTAAAWDSPESHIGVAKKLEKEIPHAVILDQYNNMKNPEAHYQGTGKEIQGQLQQLGEYENLHGVFAGAGTGGTISGISKYLKEQNPEIKIIGADPEGSILAQPESLNETEAAEYKVEGIGYDFIPKVLDRGLVDTWYKTNDIDSFRYARQLIANEGLLVGGSSGSAFSAMMKYCKDHPELTEKDVLVIILPDSIRSYLTKFADDEWLKKNNLWDDDILKPLGRSQKDIFKGATVKDLNLKPVVSVKENASTSDVVKILTDNGFDQLPVLTEDGKLCGLVTLSQLLKGLSSGEEVSSIKGHFFDFRKLNNFDEVSSYNENKSGKKKFVNFDVNSKLSDLNQFFEKNSSAVITDGLKPIHIVTKMDLLAFLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGD3</accession>
    <name>C5DGD3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Kinetochore protein Spc24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0D04378g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the NDC80 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Chromosome</location>
            <location evidence="10">Centromere</location>
            <location evidence="10">Kinetochore</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the SPC24 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552913.1">
        <property type="nucleotide sequence ID" value="XM_002552867.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGD3"/>
    <dbReference type="FunCoup" id="C5DGD3">
        <property type="interactions" value="161"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGD3"/>
    <dbReference type="GeneID" id="8295139"/>
    <dbReference type="KEGG" id="lth:KLTH0D04378g"/>
    <dbReference type="eggNOG" id="ENOG502S3GS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095757_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGD3"/>
    <dbReference type="OMA" id="ENMPPNA"/>
    <dbReference type="OrthoDB" id="3344830at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031262">
        <property type="term" value="C:Ndc80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11565">
        <property type="entry name" value="RWD_Spc24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013252">
        <property type="entry name" value="Ndc80_Spc24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038066">
        <property type="entry name" value="Spc24_Fungi_globular_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22142">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22142:SF2">
        <property type="entry name" value="KINETOCHORE PROTEIN SPC24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08286">
        <property type="entry name" value="Spc24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143026">
        <property type="entry name" value="Kinetochore globular domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 10" id="KW-0132">Cell division</keyword>
    <keyword evidence="9 10" id="KW-0137">Centromere</keyword>
    <keyword evidence="2 10" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 10" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="4 10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="7 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="30"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU368011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR22475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="197" mass="22357" checksum="BAF56064CE87C603" modified="2009-07-28" version="1">MSEEALIENPSNLIRETRTNFSIVNDTEALSNISKNLNELQRIVKEKLDRKSNELSQSESALEKNEARLDTLRRARTESGSGQLQGGSSEDVLEMARELESLERRLVELRQEVDRGLEQLIEKNADDGKVQELEESQTEDPEHRTNVLKIQLYRSLGVVLDADNNRALISKDGTLDVVPVGGDASAYFRTKYLWDRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGH1</accession>
    <name>C5DGH1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">glutaminase</fullName>
            <ecNumber evidence="2">3.5.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0D05280g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutaminase PdxT/SNO family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.2" evidence="2"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552951.1">
        <property type="nucleotide sequence ID" value="XM_002552905.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGH1"/>
    <dbReference type="FunCoup" id="C5DGH1">
        <property type="interactions" value="231"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGH1"/>
    <dbReference type="GeneID" id="8295179"/>
    <dbReference type="KEGG" id="lth:KLTH0D05280g"/>
    <dbReference type="eggNOG" id="KOG3210">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069674_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGH1"/>
    <dbReference type="OMA" id="GMIMLAD"/>
    <dbReference type="OrthoDB" id="2039at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903600">
        <property type="term" value="C:glutaminase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042823">
        <property type="term" value="P:pyridoxal phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008614">
        <property type="term" value="P:pyridoxine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01749">
        <property type="entry name" value="GATase1_PB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01615">
        <property type="entry name" value="PdxT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002161">
        <property type="entry name" value="PdxT/SNO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021196">
        <property type="entry name" value="PdxT/SNO_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03800">
        <property type="entry name" value="PLP_synth_Pdx2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31559">
        <property type="entry name" value="PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31559:SF0">
        <property type="entry name" value="PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01174">
        <property type="entry name" value="SNO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005639">
        <property type="entry name" value="Glut_amidoT_SNO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01236">
        <property type="entry name" value="PDXT_SNO_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51130">
        <property type="entry name" value="PDXT_SNO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="114"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="231"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="233"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="81"/>
            <end position="83"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="185"/>
            <end position="186"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR005639-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR005639-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR22513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="254" mass="27310" checksum="188A22EAEB9F42D4" modified="2009-07-28" version="1">MSRVKSSIASYETKCVTSDANNIAHNGKSRTVGVLALQGAFAEHVEYLEACIAQHGYPVHVMPVRTAAQLERCDALVIPGGESTAMTQIASRMGLHEALARFVQDPRRSVWGTCAGLILLAREAADAAPAAAPQRPAALVEPLKALEVAVRRNAFGRQAQSFVATCDFSAFLGPSETQGFPAVFIRAPVIDHVIDPERVEVLHRLAGADGAPGTGPIVAVRQGRILGTSFHPELADDTRFHDWFLRDFVLGDHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGH2</accession>
    <name>C5DGH2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0D05302p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0D05302g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552952.1">
        <property type="nucleotide sequence ID" value="XM_002552906.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGH2"/>
    <dbReference type="FunCoup" id="C5DGH2">
        <property type="interactions" value="117"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGH2"/>
    <dbReference type="GeneID" id="8295180"/>
    <dbReference type="KEGG" id="lth:KLTH0D05302g"/>
    <dbReference type="eggNOG" id="ENOG502QRJV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046445_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGH2"/>
    <dbReference type="OrthoDB" id="4032425at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR22514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="378" mass="44334" checksum="2956F452F621A022" modified="2009-07-28" version="1">MNGQLTQLRPPTNYELFAEAYPVYKVNKKRKRNRELWTKLHKFVELFEYLPGFVDLWLEYMECLRYDSITLDYMRMNRELDGKLTELEWIVVGSEAKLAMFSREGVLQVQYQIKEYHKFIDSEFRPRVTMNTAHLDEGAEAPSVVRALQKLELLDLVNSVVFYQDEDSASEETILQMVANLEALRGLESVRVVGQMLFERVVNFHGVRDHPGRTIGYVVRKRVSQLLVERCASIGGKRNIADFTRWDHVSRIKLSKVRELDLTRTMLPLGCRWLRLENIDKLKWWEQRTVEAELPESCLVCSNPPRAGEPAVWQVDRAAMGEEVLLRCQALLWEIYGRLSCLQLINVREIDRAPVVPRAWRGARFYCWRSMVSRITFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGM9</accession>
    <name>C5DGM9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">KLTH0D06622p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0D06622g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553009.1">
        <property type="nucleotide sequence ID" value="XM_002552963.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGM9"/>
    <dbReference type="FunCoup" id="C5DGM9">
        <property type="interactions" value="723"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGM9"/>
    <dbReference type="GeneID" id="8295240"/>
    <dbReference type="KEGG" id="lth:KLTH0D06622g"/>
    <dbReference type="eggNOG" id="KOG0756">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015166_5_1_1"/>
    <dbReference type="InParanoid" id="C5DGM9"/>
    <dbReference type="OMA" id="AWYAGCT"/>
    <dbReference type="OrthoDB" id="44467at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071913">
        <property type="term" value="F:citrate secondary active transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006843">
        <property type="term" value="P:mitochondrial citrate transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000007">
        <property type="entry name" value="Mitochondrial tricarboxylate transport protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049563">
        <property type="entry name" value="TXTP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45788">
        <property type="entry name" value="SUCCINATE/FUMARATE MITOCHONDRIAL TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45788:SF4">
        <property type="entry name" value="TRICARBOXYLATE TRANSPORT PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="7"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="106"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="209"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR22571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="297" mass="32039" checksum="4FD0408B2AE42ABE" modified="2009-07-28" version="1">MSQKRQVDPLHSFLAGALAGAAEASITYPFEFAKTRLQLVDKSSTASRNPLTLIYRTAKSQGVGALYVGCPAFIVGNTAKAGVRFLGFDAIKNVLRDPQTGELSGPRGIVAGLGAGLLESVVAVTPFEAIKTALIDDKQSANPKYHNNGRGMLRNYSNLVYDQGFSGLYRGVLPVSMRQAANQAVRLGCYNKIKTMIQEYTNAPKDKPLSSGLTFVVGAFSGIVTVYTTMPIDTVKTRMQSLNSSKYSSTLNCFSTVFREEGLKTFWKGATPRLGRLILSGGIVFTIYEKVLIILGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGN4</accession>
    <name>C5DGN4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Malic enzyme</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0D06732g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(S)-malate + NAD(+) = pyruvate + CO2 + NADH</text>
            <dbReference type="Rhea" id="RHEA:12653"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.38"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>oxaloacetate + H(+) = pyruvate + CO2</text>
            <dbReference type="Rhea" id="RHEA:15641"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="EC" id="1.1.1.38"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="10">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="10">Divalent metal cations. Prefers magnesium or manganese.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the malic enzymes family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553014.1">
        <property type="nucleotide sequence ID" value="XM_002552968.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGN4"/>
    <dbReference type="FunCoup" id="C5DGN4">
        <property type="interactions" value="955"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGN4"/>
    <dbReference type="GeneID" id="8295245"/>
    <dbReference type="KEGG" id="lth:KLTH0D06732g"/>
    <dbReference type="eggNOG" id="KOG1257">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011405_5_2_1"/>
    <dbReference type="InParanoid" id="C5DGN4"/>
    <dbReference type="OMA" id="QIVNHMV"/>
    <dbReference type="OrthoDB" id="5365701at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004471">
        <property type="term" value="F:malate dehydrogenase (decarboxylating) (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006108">
        <property type="term" value="P:malate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05312">
        <property type="entry name" value="NAD_bind_1_malic_enz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10380:FF:000001">
        <property type="entry name" value="NAD-dependent malic enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000055">
        <property type="entry name" value="NAD-dependent malic enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10380">
        <property type="entry name" value="Malic enzyme, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015884">
        <property type="entry name" value="Malic_enzyme_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012301">
        <property type="entry name" value="Malic_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037062">
        <property type="entry name" value="Malic_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012302">
        <property type="entry name" value="Malic_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001891">
        <property type="entry name" value="Malic_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010052">
        <property type="entry name" value="PRK13529.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23406">
        <property type="entry name" value="MALIC ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23406:SF34">
        <property type="entry name" value="NAD-DEPENDENT MALIC ENZYME, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00390">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03949">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000106">
        <property type="entry name" value="ME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00072">
        <property type="entry name" value="MALOXRDTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01274">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00919">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00331">
        <property type="entry name" value="MALIC_ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="4 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Malic enzyme N-terminal" evidence="13">
        <location>
            <begin position="150"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="Malic enzyme NAD-binding" evidence="12">
        <location>
            <begin position="341"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="173"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="245"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="316"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="317"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="340"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="485"/>
        </location>
        <ligand>
            <name>(S)-malate</name>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="525"/>
        </location>
        <ligand>
            <name>(S)-malate</name>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00050168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00052591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000106-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000106-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000106-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR22576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="653" mass="72045" checksum="4D0165EA5FA95602" modified="2009-07-28" version="1">MSARVESVIKTSIRIGRLARLYTSGSPMNLASTTRDNTFGNGAECGDPAHLKLEPVGSQAKAMLKDPVPKQTRLFVEGPIECPMEGFQLLNSPLFNKGSAFTQEEREAFGLEGLLPPMVNTLDEQVERAYKQLCYLKTPLAKNDFMTSMRVQNKVLYFELVRRHIRELVPIIYTPTEGDAIAAYSHRFRKPEGVFLDITEPDSIERRLAAYGTSKDVDYVVVSDSEGILGIGDQGIGGIRIAISKLALMTLCAGIHPGRVLPVCLDVGTNNKKLARDELYMGNRFSRIRGAQYDEFVDKFIKALKKRFPSAVLHFEDFGVTTARPLLDRYRGELACFNDDIQGTGAVVMASLLAALKHTKRELADVKVLVYGAGSAGLGIADQIKNHMVTYGLTVEEARSKIYLMDRRGLIMKSMESSSSPAQFLYAKPDEKWSGVNTSSLLEVVSKVKPTCLIGCSTQAGAFNKAVVQEMHKHNPRPIIFPLSNPTRLHEAVPEDLMKWTNFEALVATGSPFPPVEGYRISENNNCFSFPGIGLGAVLSRATAITDTMISAAVDQLAALSPMTENDSKPGLLPPLEVINDTSSKVATAVILQALKEGTARIEEEDVPGSEEKVKVPRDFESCLKWVTKQMWKPEYRPMVKVQHDPNVFTHQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGU3</accession>
    <name>C5DGU3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Transcription initiation factor IIA subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0D08338g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. TFIIA in a complex with TBP mediates transcriptional activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">TFIIA is a heterodimer composed of the large TOA1 and the small TOA2 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TFIIA subunit 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553073.1">
        <property type="nucleotide sequence ID" value="XM_002553027.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGU3"/>
    <dbReference type="FunCoup" id="C5DGU3">
        <property type="interactions" value="553"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGU3"/>
    <dbReference type="GeneID" id="8295309"/>
    <dbReference type="KEGG" id="lth:KLTH0D08338g"/>
    <dbReference type="eggNOG" id="KOG3463">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112964_3_1_1"/>
    <dbReference type="InParanoid" id="C5DGU3"/>
    <dbReference type="OMA" id="QYYELYR"/>
    <dbReference type="OrthoDB" id="586585at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005672">
        <property type="term" value="C:transcription factor TFIIA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10014">
        <property type="entry name" value="TFIIA_gamma_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10145">
        <property type="entry name" value="TFIIA_gamma_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.190:FF:000001">
        <property type="entry name" value="Transcription initiation factor IIA subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.18.10:FF:000003">
        <property type="entry name" value="Transcription initiation factor IIA subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.18.10">
        <property type="entry name" value="Transcription factor IIA (TFIIA), beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.190">
        <property type="entry name" value="Transcription factor IIA gamma subunit, alpha-helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009083">
        <property type="entry name" value="TFIIA_a-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009088">
        <property type="entry name" value="TFIIA_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003194">
        <property type="entry name" value="TFIIA_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015871">
        <property type="entry name" value="TFIIA_gsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015872">
        <property type="entry name" value="TFIIA_gsu_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10966">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIA SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02751">
        <property type="entry name" value="TFIIA_gamma_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02268">
        <property type="entry name" value="TFIIA_gamma_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009415">
        <property type="entry name" value="Hum_TFIIA_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47396">
        <property type="entry name" value="Transcription factor IIA (TFIIA), alpha-helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50784">
        <property type="entry name" value="Transcription factor IIA (TFIIA), beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription initiation factor IIA gamma subunit N-terminal" evidence="10">
        <location>
            <begin position="6"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription initiation factor IIA gamma subunit C-terminal" evidence="11">
        <location>
            <begin position="63"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00063181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR009415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR22635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="115" mass="12947" checksum="37C5A4FF8241A47F" modified="2009-07-28" version="1">MSASGYYELYRRSTVGATLVDALDTLISDGRIEASLAMRVLDTFDRVVAETLKDKTQSKLTFKGHLDTYRFCDDVWTFIIKDCNVKLDQTEDIQGNTVVVDKLRIVACNSKKTGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGW9</accession>
    <name>C5DGW9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">KLTH0D08932p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0D08932g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">The S.cerevisiae Ras proteins modulate the activity of the adenylate cyclase catalytic subunit and therefore affect the biosynthesis of cyclic-AMP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Ras family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553099.1">
        <property type="nucleotide sequence ID" value="XM_002553053.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGW9"/>
    <dbReference type="FunCoup" id="C5DGW9">
        <property type="interactions" value="524"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGW9"/>
    <dbReference type="GeneID" id="8295336"/>
    <dbReference type="KEGG" id="lth:KLTH0D08932g"/>
    <dbReference type="eggNOG" id="KOG0395">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041217_9_0_1"/>
    <dbReference type="InParanoid" id="C5DGW9"/>
    <dbReference type="OMA" id="WHVEVID"/>
    <dbReference type="OrthoDB" id="5976022at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097271">
        <property type="term" value="P:protein localization to bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000080">
        <property type="entry name" value="Ras-like GTPase Ras1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020849">
        <property type="entry name" value="Small_GTPase_Ras-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24070">
        <property type="entry name" value="RAS, DI-RAS, AND RHEB FAMILY MEMBERS OF SMALL GTPASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0564">Palmitate</keyword>
    <keyword evidence="10" id="KW-0636">Prenylation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="187"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="228"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00059851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR22661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="292" mass="31208" checksum="770369577D3051F4" modified="2009-07-28" version="1">MSLNKNNIREYKLVVVGGGGVGKSALTIQLIQSHFVDEYDPTIEDSYRKQVVVDGKVSILDILDTAGQEEYSAMREQYMRTGEGFLLVYSVTSRTSFEELMTYYQQILRVKDADYVPVFLVGNKSDLEDERQVAYEEGVSLAKQFNAPFLETSAKQAINVEESFYGLVRLVRDEGAVYNPAHGEQLGQKFQQQQARNQSMGAGAGLGVGASDGLDNGGHASDPNMLSADAQGGQAADARTSGVAASGKAGELKSGKAPGAAGKVKSPAGRVEQRADNTSGKKQKSGGCCIVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DH42</accession>
    <name>C5DH42_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Kinetochore protein NDC80</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0E01188g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the NDC80 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Chromosome</location>
            <location evidence="10">Centromere</location>
            <location evidence="10">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the NDC80/HEC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553540.1">
        <property type="nucleotide sequence ID" value="XM_002553494.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DH42"/>
    <dbReference type="FunCoup" id="C5DH42">
        <property type="interactions" value="348"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DH42"/>
    <dbReference type="GeneID" id="8291678"/>
    <dbReference type="KEGG" id="lth:KLTH0E01188g"/>
    <dbReference type="eggNOG" id="KOG0995">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012583_1_2_1"/>
    <dbReference type="InParanoid" id="C5DH42"/>
    <dbReference type="OMA" id="PSHKFQK"/>
    <dbReference type="OrthoDB" id="7459479at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031262">
        <property type="term" value="C:Ndc80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051315">
        <property type="term" value="P:attachment of mitotic spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.30:FF:000001">
        <property type="entry name" value="Probable kinetochore protein ndc80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.30">
        <property type="entry name" value="Ncd80 complex, Ncd80 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040967">
        <property type="entry name" value="DUF5595"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005550">
        <property type="entry name" value="Kinetochore_Ndc80"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055260">
        <property type="entry name" value="Ndc80_CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038273">
        <property type="entry name" value="Ndc80_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10643">
        <property type="entry name" value="KINETOCHORE PROTEIN NDC80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10643:SF2">
        <property type="entry name" value="KINETOCHORE PROTEIN NDC80 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18077">
        <property type="entry name" value="DUF5595"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03801">
        <property type="entry name" value="Ndc80_HEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 10" id="KW-0132">Cell division</keyword>
    <keyword evidence="9 10" id="KW-0137">Centromere</keyword>
    <keyword evidence="2 10" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 10" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="4 10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="7 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinetochore protein Ndc80 CH" evidence="13">
        <location>
            <begin position="95"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="DUF5595" evidence="14">
        <location>
            <begin position="262"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="58"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="372"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="517"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="60"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="84"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU368072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF03801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF18077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR23103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="676" mass="78477" checksum="C711C8736D704233" modified="2009-07-28" version="1">MEESRKRLSNESHAVLGHLDPQRFTSQIPMAVNGRRRNSTASAIGGAGLSELINRSMAKNKPEVPKRSYTDSRRSLIADRKSLRSSQRTSLLPNHGLNSANTTNTNNKDPRPLRDKNFQVAIQQEIYDYLHSQKFDVQTNHPISLKSLRQPTQKDFVFIFRWLYQRMDPGYKFSKSIEHEVYTILRAIQYPYLETINKSQISAVGGSSWPKFLGMLHWLVNINKRLDSYLQKVDLTLISQNTQDITVLQQPIGTIDEQEDKQEKYELIVERLFIDYIVQSYKSFLRLDDDYDQYMKELELGFEKFAQVIRSDIANIGMQNSDLSVKYQELVGKGQDVKVAKDKFNALRSDLTKFQNYVNAMQHKSQEWPKKLEKMETERENKMSQLKATENQITEFRDLIKGKNIEIAEVDRLNAERERLTRSLDSVSTDMDQAVSLLKAQKMEAESAYKTLLDVGKQYESAVESLVVARLNLGHQLNTDNRKISLPDTFYSRGYEPGQSGLDNDIPPEMDVRGTIKPKLLELNEEVRSRVQKLQQENASFEAHLENLKQEISEKTRYLESLENELSIAKSQYDEQQQESQSHLLSQRIEIEKMERKLQNVRYSSHQKIAEAEQKVQSTKLKYEELKLAVNRERNILHGKVIQLIDYVSDFKINVQSAVEDLDYLAIEELNKIKFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHH1</accession>
    <name>C5DHH1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0E04290p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0E04290g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553669.1">
        <property type="nucleotide sequence ID" value="XM_002553623.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHH1"/>
    <dbReference type="FunCoup" id="C5DHH1">
        <property type="interactions" value="113"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHH1"/>
    <dbReference type="GeneID" id="8291818"/>
    <dbReference type="KEGG" id="lth:KLTH0E04290g"/>
    <dbReference type="eggNOG" id="ENOG502RC15">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_392821_0_0_1"/>
    <dbReference type="InParanoid" id="C5DHH1"/>
    <dbReference type="OMA" id="YKNDYFH"/>
    <dbReference type="OrthoDB" id="10256089at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031991">
        <property type="term" value="P:regulation of actomyosin contractile ring contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032955">
        <property type="term" value="P:regulation of division septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051492">
        <property type="entry name" value="Dynamin-Rho_GEF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22834">
        <property type="entry name" value="NUCLEAR FUSION PROTEIN FUS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22834:SF20">
        <property type="entry name" value="SH3 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="1">
        <location>
            <begin position="124"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="657" mass="75541" checksum="0EDFB8AEF44C5188" modified="2009-07-28" version="1">MIKSTNSIYQLDYPAKSTLTPIRDSREAFFPNRGSSAYKNGSQLRETLPFNPYAKSMWTSECHYKASAKYPVAAKTHSIHTKPPKSRGFQRSASILISSTEPLNLDFEKAPTKPASTSEFVGKKFFDAFYDFLGREEDYAYMTDMLNMIYRKELHHKKASGKLMAQSSNEEILLFGNMDTISQLSRILVKSLRNYVSSCCKSGTACARWSGPDASFEIPESFAEHFDPAEFFESHLSKIRSTYSTYFLSHRRQLELLEHLKKTKSTLFYRWYESCLDKADLVKIEDIIAAPVKRLGEFYKDILNIIREGEDFLSGSALEKLRMFADKFHSFLSTAETLLDEGAVAKEQAPFDSRSSNISNHLTVPLEGNSCRSSETHLSLSSSRYSDHLSVGDMDAAVISSHVKLNEAENEDPTLADYISRFKQVGKHMEKLEKELSDLDIAAILDQNMRQAEEWRKIFEFEPLSQLLTEQPNVESIYTIYINKLHQQRQEVMLTKLGDLQEKVIAPLTLAKQSSKMIQSKIQDLKELKKDYMSFLGSKDARSIKFKLIANHFQSTQRELLDGIPKYLDLISQFTILLMRSYNQIMMKYMEILSGGKRLLTRELQMLEKGEREQGDNFDILQSFASSRFYTKQLIHENWNCHGRAVESRVVRKLFEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHJ7</accession>
    <name>C5DHJ7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Riboflavin synthase</fullName>
            <ecNumber evidence="5">2.5.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0E04862g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the dismutation of two molecules of 6,7-dimethyl-8-ribityllumazine, resulting in the formation of riboflavin and 5-amino-6-(D-ribitylamino)uracil.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 6,7-dimethyl-8-(1-D-ribityl)lumazine + H(+) = 5-amino-6-(D-ribitylamino)uracil + riboflavin</text>
            <dbReference type="Rhea" id="RHEA:20772"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
            <dbReference type="ChEBI" id="CHEBI:57986"/>
            <dbReference type="ChEBI" id="CHEBI:58201"/>
            <dbReference type="EC" id="2.5.1.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotrimer.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.9" evidence="5"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553695.1">
        <property type="nucleotide sequence ID" value="XM_002553649.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHJ7"/>
    <dbReference type="FunCoup" id="C5DHJ7">
        <property type="interactions" value="201"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHJ7"/>
    <dbReference type="GeneID" id="8291844"/>
    <dbReference type="KEGG" id="lth:KLTH0E04862g"/>
    <dbReference type="eggNOG" id="KOG3310">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034388_1_1_1"/>
    <dbReference type="InParanoid" id="C5DHJ7"/>
    <dbReference type="OMA" id="HFVTGHV"/>
    <dbReference type="OrthoDB" id="10258924at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004746">
        <property type="term" value="F:riboflavin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00402">
        <property type="entry name" value="Riboflavin_synthase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.20:FF:000004">
        <property type="entry name" value="Riboflavin synthase, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.20:FF:000006">
        <property type="entry name" value="Riboflavin synthase, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023366">
        <property type="entry name" value="ATP_synth_asu-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001783">
        <property type="entry name" value="Lumazine-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026017">
        <property type="entry name" value="Lumazine-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006767">
        <property type="entry name" value="PRK09289.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00187">
        <property type="entry name" value="ribE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21098:SF0">
        <property type="entry name" value="RIBOFLAVIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21098">
        <property type="entry name" value="RIBOFLAVIN SYNTHASE ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00677">
        <property type="entry name" value="Lum_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000498">
        <property type="entry name" value="Riboflavin_syn_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51177">
        <property type="entry name" value="LUMAZINE_BIND"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0686">Riboflavin biosynthesis</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Lumazine-binding" evidence="11">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="repeat" description="Lumazine-binding" evidence="10">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="Lumazine-binding" evidence="11">
        <location>
            <begin position="104"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="repeat" description="Lumazine-binding" evidence="10">
        <location>
            <begin position="104"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR23258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="235" mass="25598" checksum="2E2FCBC214B3F0B5" modified="2009-07-28" version="1">MFTGIVELIGTVAEYKEYDTTHSGGGGVSVTVKDASPILGDCHIGDSIAVNGICLTVTEYSSDSFKVGISPETLRRTNVASWQPGSKVNLERAVGQDVRFGGHYVQGHIDGTAKVVSRVAEGNSIVFGFKLHEKEFEKYIVEKGFICIDGTSLTITKVEPDGTFYIAMIKHTQEKVVMPLKTVGDEVNIEVDLTGKVIEKQVVLQLQEQISNEHSPLAILVDKLVEAKVKKFLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHK3</accession>
    <name>C5DHK3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0E04994p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0E04994g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553701.1">
        <property type="nucleotide sequence ID" value="XM_002553655.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHK3"/>
    <dbReference type="FunCoup" id="C5DHK3">
        <property type="interactions" value="518"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHK3"/>
    <dbReference type="GeneID" id="8291850"/>
    <dbReference type="KEGG" id="lth:KLTH0E04994g"/>
    <dbReference type="eggNOG" id="ENOG502QQ6A">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022497_1_0_1"/>
    <dbReference type="InParanoid" id="C5DHK3"/>
    <dbReference type="OMA" id="PYMYYYP"/>
    <dbReference type="OrthoDB" id="3364649at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000444">
        <property type="term" value="C:MIS12/MIND type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013218">
        <property type="entry name" value="Dsn1/Mis13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14778">
        <property type="entry name" value="KINETOCHORE-ASSOCIATED PROTEIN DSN1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14778:SF2">
        <property type="entry name" value="KINETOCHORE-ASSOCIATED PROTEIN DSN1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08202">
        <property type="entry name" value="MIS13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="112"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="172"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="371"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="403"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="119"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="173"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="188"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="371"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="385"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR23264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="542" mass="63088" checksum="4C33F3378EB886FD" modified="2009-07-28" version="1">MSRERPRSLTPQGRASQRKHGLKVQTLPLPPELKSYDSVRTTQELEENGEPDEYRRNNPQSSFEKDEDFKFKRHKHRNNAGTASLGERLDSLHELQNARWVDNFNSSVNMDRRARNSQRENPSQESQRSIPALPAQSIPPSYMPYMYYYPFAPMVHPMSTSPARAANGEMPQYISSSQGYPNTSTQPFMPPMPPMPQPNTQLMPPPPLYPNYSSYNYYNNANEDVAKKSQRRREKRSSLMAQRGRRLSLLSFQENSQIISPHKDVPERDFYRHIANTSFGQDLQIRQLFSWCLIRCLRKWESNEHQPSREEAKSNEETYADPKKIALTIVKEFVEELRKGKTDVNWDAEEYTDSSTEDNTQFQEEDTELRELFDEDDDDTDILHSRPRKRRRQQPLKIPNEKNVENAKNLKVLEKQISSLEEEIKTWIQELDKIDHTSEWRSFKSQLIKLATAEIKPASPSPLLHDLKTELECRVESLRVTSHFLHSNSELLSLSTQQKVQKLTQCINNGVFSARTDPQTNKHTTRNLLRGLSRSLTKEDSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHN2</accession>
    <name>C5DHN2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0E05720p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0E05720g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553730.1">
        <property type="nucleotide sequence ID" value="XM_002553684.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHN2"/>
    <dbReference type="FunCoup" id="C5DHN2">
        <property type="interactions" value="11"/>
    </dbReference>
    <dbReference type="GeneID" id="8291883"/>
    <dbReference type="KEGG" id="lth:KLTH0E05720g"/>
    <dbReference type="eggNOG" id="ENOG502SDDG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114192_0_0_1"/>
    <dbReference type="InParanoid" id="C5DHN2"/>
    <dbReference type="OMA" id="CDTDANV"/>
    <dbReference type="OrthoDB" id="4031501at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019787">
        <property type="term" value="F:ubiquitin-like protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1460.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007135">
        <property type="entry name" value="Atg3/Atg10"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03987">
        <property type="entry name" value="Autophagy_act_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0072">Autophagy</keyword>
    <keyword evidence="2" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR23293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="158" mass="18207" checksum="0514BA174848FC01" modified="2009-07-28" version="1">MIPLGEFREQLQALIPVLQLWPKCLSVHASLNGEALIGLAEHPTLAFYTYEFEIGYSQTYQEPRLIFKIWEATTEQGAELQRPCFPADLSRLMNVQDFSIGLDHLHEARKDSWFSVHACDTSHIVGSVKHHYLKRWASVYLSLFDPCFSNTYLFVDDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHT6</accession>
    <name>C5DHT6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Mitochondrial aspartate-glutamate transporter AGC1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Aspartate-glutamate carrier 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">KLTH0E06996g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Calcium-dependent mitochondrial aspartate and glutamate carrier. Transport of glutamate in mitochondria is required for mitochondrial transamination reactions and ornithine synthesis. Plays also a role in malate-aspartate NADH shuttle, which is critical for growth on acetate and fatty acids.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553784.1">
        <property type="nucleotide sequence ID" value="XM_002553738.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHT6"/>
    <dbReference type="FunCoup" id="C5DHT6">
        <property type="interactions" value="19"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHT6"/>
    <dbReference type="GeneID" id="8291939"/>
    <dbReference type="KEGG" id="lth:KLTH0E06996g"/>
    <dbReference type="eggNOG" id="KOG0751">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014931_1_0_1"/>
    <dbReference type="InParanoid" id="C5DHT6"/>
    <dbReference type="OMA" id="YYYKSCQ"/>
    <dbReference type="OrthoDB" id="2161at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015183">
        <property type="term" value="F:L-aspartate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005313">
        <property type="term" value="F:L-glutamate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043490">
        <property type="term" value="P:malate-aspartate shuttle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000004">
        <property type="entry name" value="Calcium-binding mitochondrial carrier protein Aralar1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051028">
        <property type="entry name" value="Mito_Solute_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45678:SF9">
        <property type="entry name" value="CALCIUM-BINDING MITOCHONDRIAL CARRIER PROTEIN ARALAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45678">
        <property type="entry name" value="MITOCHONDRIAL 2-OXODICARBOXYLATE CARRIER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="15">
        <location>
            <begin position="498"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="15">
        <location>
            <begin position="592"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="15">
        <location>
            <begin position="694"/>
            <end position="782"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00059916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00073787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00082232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAR23347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="882" mass="101975" checksum="41834C937D5360BB" modified="2009-07-28" version="1">MELSNSITEKKNQQVEIFQKFATELKRGDSGDSSKLVLTFNDFIDLISSSRSLYSRFTDHSYNLNQIPDKAFGCIFFAVDENNKGYLTLNDWFHFNNLLECNNYHLIILYEFFRKFDVANLKRKAASIENSSPSAINIANNPRIRPPNYGSKFLSFDSLYLDLTQFKSTIKFLQECVDNEFITANNLFLNWDEFRWLKFYQSFPYGLREGSHINLNSLVTILQNDLKNEKLFMGFDRLSHTNHKNHSLVLNKNQLVYLLKVFYSHRISADVFNSLDLSNTKMIKSNNNYINYNVFKDIFYLFQNFDLLNQVFMRYAKTHNFDDQDLRECYISKEDLTHFLSVEYNKVNNITEFSPSQISLLFSIVANSKRNKLIEKSLNTHHEDSTIERFIQTDFCHGMSPKNQELKEFNNNYREVIGGMSGELDTAKNRRQTTKAGLIDRILKSKETDETWLEFNLTMEDFMKILNPNYLNDLVHQMEIRRIQSESLYTNFYFFPIFDSIYNFLLGSVAGCIGATAVYPIDLVKTRMQAQRNFSQYKNSIDCFVKIFSREGVRGIYSGLGPQLVGVAPEKAIKLTVNDYVRKMLMDSNNHLTLPLEILSGASAGACQVIFTNPLEIVKIRLQVRSEYAESISRSQVNAFGIVKSLGLRGLYRGIGACLMRDVPFSAIYFPTYAHLKKDIFNYDPQDKNGRTRLRTWELLTAGGLAGMPAAYLTTPFDVIKTRLQIDPRKGETKYNGIWHAAKTILKEERFKSFFKGGGARVLRSSPQFGFTLAAYEIFQNLFPLKHSEVNNTKQDDDTPIVTGMFSSFLESLKKSSNQNVEITEFFGPSVDPYSSNYLNYYYKSCQVAKLFIDLDYNFAKFDFGVYSKFHKFVKDAESPSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHZ0</accession>
    <name>C5DHZ0_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein-lysine N-methyltransferase EFM5</fullName>
            <ecNumber evidence="5">2.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Elongation factor methyltransferase 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">EFM5</name>
        <name evidence="7" type="ordered locus">KLTH0E08294g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">S-adenosyl-L-methionine-dependent protein-lysine N-methyltransferase that trimethylates elongation factor 1-alpha at 'Lys-79'.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the class I-like SAM-binding methyltransferase superfamily. EFM5 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="5"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553838.1">
        <property type="nucleotide sequence ID" value="XM_002553792.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHZ0"/>
    <dbReference type="FunCoup" id="C5DHZ0">
        <property type="interactions" value="315"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHZ0"/>
    <dbReference type="GeneID" id="8291998"/>
    <dbReference type="KEGG" id="lth:KLTH0E08294g"/>
    <dbReference type="eggNOG" id="KOG3350">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074410_1_0_1"/>
    <dbReference type="InParanoid" id="C5DHZ0"/>
    <dbReference type="OMA" id="CNFRPEH"/>
    <dbReference type="OrthoDB" id="206354at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03187">
        <property type="entry name" value="Methyltr_EFM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019369">
        <property type="entry name" value="Efm5/EEF1AKMT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041370">
        <property type="entry name" value="Mlase_EEF1AKMT1/ZCCHC4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13200">
        <property type="entry name" value="EEF1A LYSINE METHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13200:SF0">
        <property type="entry name" value="EEF1A LYSINE METHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10237">
        <property type="entry name" value="N6-adenineMlase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0808">Transferase</keyword>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="20"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR23401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="255" mass="29283" checksum="590F05C843DE3D19" modified="2009-07-28" version="1">MEDVELSLSPEALKALLEFQQEEEEREKKLKSLYADAEKKFEEVKAQEGMRVFKEDWQLSQFWYDEETANNLAQALLEGANENTVIAILSAPSVYVAISKMSPSCVPTKHIYLFEYDKRFEVLAGSEHFCFYDYTNPLEFPASLDGKVHRILIDPPFLNKPCQTKCKLQLQVLHLTSVLTFHQVGVTANKLLAKANDCGEQERRIMISTGERMAEVVSETYPGTRKTTFTPSHANGLSNEFECYANFEWRGWKFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DI05</accession>
    <name>C5DI05_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pre-mRNA-splicing factor 18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0E08668g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PRP18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553853.1">
        <property type="nucleotide sequence ID" value="XM_002553807.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DI05"/>
    <dbReference type="FunCoup" id="C5DI05">
        <property type="interactions" value="594"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DI05"/>
    <dbReference type="GeneID" id="8292014"/>
    <dbReference type="KEGG" id="lth:KLTH0E08668g"/>
    <dbReference type="eggNOG" id="KOG2808">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081028_0_0_1"/>
    <dbReference type="InParanoid" id="C5DI05"/>
    <dbReference type="OMA" id="WITSTKR"/>
    <dbReference type="OrthoDB" id="10261918at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071021">
        <property type="term" value="C:U2-type post-spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000350">
        <property type="term" value="P:generation of catalytic spliceosome for second transesterification step"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.940.10">
        <property type="entry name" value="Functional domain of the splicing factor Prp18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004098">
        <property type="entry name" value="Prp18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039979">
        <property type="entry name" value="PRPF18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13007">
        <property type="entry name" value="PRE-MRNA SPLICING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13007:SF19">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02840">
        <property type="entry name" value="Prp18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47938">
        <property type="entry name" value="Functional domain of the splicing factor Prp18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Prp18" evidence="9">
        <location>
            <begin position="146"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="16"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="20"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="54"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="69"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR23416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="265" mass="30246" checksum="5C7BF27970598E6B" modified="2009-07-28" version="1">MDYANLLKAEIAKKQKAIASSNRPSETPWLTQQQDEGDGLESKMLRSTEAQDNQEQKPPLEHDVREVFREQGKSKETDSKSAASASRLRTRPGRIAKVLADERKLDTAISATEIGDVEQEAHLSLKCNLYIHLLLQLWQEECYKPELILETKKALYPLVLKLRKRRLSSEFLTSLATILFHTQQHQFPQATQSYMKLSIGNVAWPIGVTSVGIHARSAHERIQGKDKIANVMLDEHTRLWITSVKRLITFAEALERRKKSQHANE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DI33</accession>
    <name>C5DI33_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cytochrome c oxidase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0E09350g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytochrome c oxidase subunit 6B family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553881.1">
        <property type="nucleotide sequence ID" value="XM_002553835.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DI33"/>
    <dbReference type="FunCoup" id="C5DI33">
        <property type="interactions" value="472"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DI33"/>
    <dbReference type="GeneID" id="8292045"/>
    <dbReference type="KEGG" id="lth:KLTH0E09350g"/>
    <dbReference type="eggNOG" id="KOG3057">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_133964_2_0_1"/>
    <dbReference type="InParanoid" id="C5DI33"/>
    <dbReference type="OMA" id="NEWIAKW"/>
    <dbReference type="OrthoDB" id="1107506at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00926">
        <property type="entry name" value="Cyt_c_Oxidase_VIb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.140:FF:000001">
        <property type="entry name" value="Cytochrome c oxidase subunit 6B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.140">
        <property type="entry name" value="Cytochrome c oxidase, subunit VIb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048280">
        <property type="entry name" value="COX6B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036549">
        <property type="entry name" value="CX6/COA6-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003213">
        <property type="entry name" value="Cyt_c_oxidase_su6B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11387">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02297">
        <property type="entry name" value="COX6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000278">
        <property type="entry name" value="Cyt_c_oxidase_6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47694">
        <property type="entry name" value="Cytochrome c oxidase subunit h"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="27"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="37"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR000278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000278-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR23444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="82" mass="9714" checksum="B5E202D74407F99F" modified="2009-07-28" version="1">MSEKQESPLHTVGFDARFPNQNQTMHCWQSYVDYYKCINAKGEDFAPCKVFFRTFSSLCPLDWVERWDEQREKGTFPGDIKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIP0</accession>
    <name>C5DIP0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0E14014p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0E14014g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554088.1">
        <property type="nucleotide sequence ID" value="XM_002554042.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIP0"/>
    <dbReference type="FunCoup" id="C5DIP0">
        <property type="interactions" value="25"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIP0"/>
    <dbReference type="GeneID" id="8292257"/>
    <dbReference type="KEGG" id="lth:KLTH0E14014g"/>
    <dbReference type="eggNOG" id="KOG1950">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034860_1_0_1"/>
    <dbReference type="InParanoid" id="C5DIP0"/>
    <dbReference type="OMA" id="ILPHRVY"/>
    <dbReference type="OrthoDB" id="2014201at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050587">
        <property type="entry name" value="GNT1/Glycosyltrans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11183">
        <property type="entry name" value="GLYCOGENIN SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR23651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="486" mass="56761" checksum="1C3E4EDDC1C7A7A1" modified="2009-07-28" version="1">MRFLFKRRLRLVGAIGAVVLLISLTAKCVVQFQLNREIEHYRTFFRKHKDNIHDIYDPLNIKQIPYETIESLYQLRKTGDVPKKSPIDWGKYAYINYVTDADYLCTSLLQFKRLKEFGTKAKLVLLISSNLLEAENPKISENKQLLEKFQEVAPTQVLIKEVENIIKPHDYSPWNKSFTKLLVFNQTEFDRIVYLDNDAGVYNHMDELFFLPDYVKFAAPLTYWFVSERDLEIANSEVSSEEKSSIKLNRYIDTLATRVKEKRPIYNHLPSLPSSLFMGTKDVAKEIIESTSSASPLFNLRNKNKNAKVKFASNLMVIKPSAETFNAIVNTLLPRIANKKEKYDMDLINEGLYDLRKIIYHQFKLFRKLKTEFVPEVLSLPFGSYGLLTGSIRNEFHHTLMANDILGYERTKEKDQVPLESLIKKSKYIHYSDYPMSKPWAYSSFDHLKCNPQDADAESEDVKAEMCKCWNSVYQDYWDSKHFCTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIY6</accession>
    <name>C5DIY6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">Hydroxymethylglutaryl-CoA synthase</fullName>
            <shortName evidence="6">HMG-CoA synthase</shortName>
            <ecNumber evidence="6">2.3.3.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">3-hydroxy-3-methylglutaryl coenzyme A synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0E16192g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the condensation of acetyl-CoA with acetoacetyl-CoA to form HMG-CoA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>acetoacetyl-CoA + acetyl-CoA + H2O = (3S)-3-hydroxy-3-methylglutaryl-CoA + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10188"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
            <dbReference type="ChEBI" id="CHEBI:57286"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.10"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the thiolase-like superfamily. HMG-CoA synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.10" evidence="6"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554184.1">
        <property type="nucleotide sequence ID" value="XM_002554138.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIY6"/>
    <dbReference type="FunCoup" id="C5DIY6">
        <property type="interactions" value="704"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIY6"/>
    <dbReference type="GeneID" id="8292356"/>
    <dbReference type="KEGG" id="lth:KLTH0E16192g"/>
    <dbReference type="eggNOG" id="KOG1393">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008065_0_1_1"/>
    <dbReference type="InParanoid" id="C5DIY6"/>
    <dbReference type="OMA" id="DDAYNWI"/>
    <dbReference type="OrthoDB" id="1269963at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004421">
        <property type="term" value="F:hydroxymethylglutaryl-CoA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006084">
        <property type="term" value="P:acetyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010142">
        <property type="term" value="P:farnesyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00827">
        <property type="entry name" value="init_cond_enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000008">
        <property type="entry name" value="3-hydroxy-3-methylglutaryl coenzyme A synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013746">
        <property type="entry name" value="HMG_CoA_synt_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013528">
        <property type="entry name" value="HMG_CoA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010122">
        <property type="entry name" value="HMG_CoA_synthase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01833">
        <property type="entry name" value="HMG-CoA-S_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43323">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL COENZYME A SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43323:SF2">
        <property type="entry name" value="HYDROXYMETHYLGLUTARYL-COA SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08540">
        <property type="entry name" value="HMG_CoA_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01154">
        <property type="entry name" value="HMG_CoA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Hydroxymethylglutaryl-coenzyme A synthase N-terminal" evidence="7">
        <location>
            <begin position="18"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="Hydroxymethylglutaryl-coenzyme A synthase C-terminal" evidence="8">
        <location>
            <begin position="190"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="4">
        <location>
            <position position="100"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="4">
        <location>
            <position position="132"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="4">
        <location>
            <position position="269"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00073381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR610122-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR610122-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR23747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="464" mass="51046" checksum="BFD97A9EB316011E" modified="2009-07-28" version="1">MTEQNQLKKQKTVSQPPRPQNIGIKAIEVYIPSQCVNQAELEKFDGVSQGKYTIGLGQTNMSFVNDREDIYSMSLTVLSKLLKNYNVDPQSVGRLEVGTETLLDKSKSVKSVLMQLFEGNTDLEGIDTVNACYGGTSALFNSLSWIESSAWDGRDAVVVCGDIAIYDKGAARPTGGAGTVAMLIGPDAPIVFDSVRGSHMEHVYDFYKPDFTSEYPYVDGHFSLSCYVSALDQAYKSYSKKAIAKGLVQDPAGPEAVNTASFFDYNVFHVPTCKLVSKSYARLLYNDFRAKPSLYPEVDASLAQVDYQTSLTDKNIEKTFVGVAKALQKERVAPSLVVPTNTGNMYTASVYAALSSLLSFVGSEALQNKRIGLYSYGSGLAASFFSCKVVGDIKYITDVLSLDKKLQSRKTETPEAYEAAIKLREDAHLKNGFKPKGSLEHLQSGVYYLTNVDERFRREYAVSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJ25</accession>
    <name>C5DJ25_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0F12914p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0F12914g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554751.1">
        <property type="nucleotide sequence ID" value="XM_002554705.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJ25"/>
    <dbReference type="FunCoup" id="C5DJ25">
        <property type="interactions" value="169"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJ25"/>
    <dbReference type="GeneID" id="8292974"/>
    <dbReference type="KEGG" id="lth:KLTH0F12914g"/>
    <dbReference type="eggNOG" id="KOG1583">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033007_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJ25"/>
    <dbReference type="OMA" id="NPFTGWH"/>
    <dbReference type="OrthoDB" id="999962at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005462">
        <property type="term" value="F:UDP-N-acetylglucosamine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005464">
        <property type="term" value="F:UDP-xylose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013657">
        <property type="entry name" value="SCL35B1-4/HUT1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00803">
        <property type="entry name" value="nst"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10778:SF4">
        <property type="entry name" value="NUCLEOTIDE SUGAR TRANSPORTER SLC35B4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10778">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08449">
        <property type="entry name" value="UAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="84"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="113"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="140"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="179"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="313"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR24314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="349" mass="38550" checksum="6C041A65C97FD789" modified="2009-07-28" version="1">MESTKFVKLRRLPMKLLLPLSSIFGGCCSNVYTLERIMASTPHNMGNIVTFMQVLFVALKTLPEFLEFRNPPTFLKRRKIPFKIHLLSVLLFLLGTVSNNSVFAFGVSVPLHIVFRSSATAITMVLSWAIGQRTYTQKQVCSAILMTTGGVVTIIYRSSEFSIESLKVANMSSVKAGEGTGLLLLISSSILLCSYSLLNDWTYRTYGKHWKESLFYMHLLSLPLLLVNWRQLREEFRYLIHGAKMVSIPFTNAKVPPAVIMMVGNVVTQSICIEGVSALASITDALTVSVILLLRKLSSLLLSVYLFDNSLSWTALIGTVTVFIGALMYILSAAPSSDIGKGSKGVKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJJ3</accession>
    <name>C5DJJ3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">P-type Cu(+) transporter</fullName>
            <ecNumber evidence="3">7.2.2.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Cu(2+)-ATPase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ordered locus">KLTH0F16874g</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="18">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 18">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.8" evidence="3"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554919.1">
        <property type="nucleotide sequence ID" value="XM_002554873.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJJ3"/>
    <dbReference type="FunCoup" id="C5DJJ3">
        <property type="interactions" value="490"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJJ3"/>
    <dbReference type="GeneID" id="8293152"/>
    <dbReference type="KEGG" id="lth:KLTH0F16874g"/>
    <dbReference type="eggNOG" id="KOG0207">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001771_0_1_1"/>
    <dbReference type="InParanoid" id="C5DJJ3"/>
    <dbReference type="OMA" id="HWMLPAW"/>
    <dbReference type="OrthoDB" id="432719at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043682">
        <property type="term" value="F:P-type divalent copper transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140581">
        <property type="term" value="F:P-type monovalent copper transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055070">
        <property type="term" value="P:copper ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030003">
        <property type="term" value="P:intracellular monoatomic cation homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd02094">
        <property type="entry name" value="P-type_ATPase_Cu-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000001">
        <property type="entry name" value="ATPase copper transporting beta"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000002">
        <property type="entry name" value="Copper-transporting ATPase 1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000043">
        <property type="entry name" value="Copper-transporting ATPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017969">
        <property type="entry name" value="Heavy-metal-associated_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006122">
        <property type="entry name" value="HMA_Cu_ion-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027256">
        <property type="entry name" value="P-typ_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01525">
        <property type="entry name" value="ATPase-IB_hvy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00003">
        <property type="entry name" value="copper ion binding protein"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43520">
        <property type="entry name" value="ATP7, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43520:SF8">
        <property type="entry name" value="P-TYPE CU(+) TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00943">
        <property type="entry name" value="CUATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01047">
        <property type="entry name" value="HMA_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0186">Copper</keyword>
    <keyword evidence="9" id="KW-0187">Copper transport</keyword>
    <keyword evidence="15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="16 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="260"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="298"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="338"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="525"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="567"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="884"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="911"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="19">
        <location>
            <begin position="5"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="19">
        <location>
            <begin position="86"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="19">
        <location>
            <begin position="168"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00080126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU362081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CAR24482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="988" mass="106436" checksum="B828D4C472FCC717" modified="2009-07-28" version="1">MGKGKVAVYHVEGMTCGACAVAIETELKRVKGVKDVTVSLATNECRVEFDGQQVEQPALKGAIMDCGFDATLAKQQQEVPSLQEQSSAVFSVQHMTCGACVATITQNISAERGVLEVDVSLATEECHVVFVPEMITPAQIKEIIDDSGFEAELINGAVATKSQSSQVRQASLKVLGMTCGSCVSTVESTLAEMPGVLSVQVSLATEEAEMQYDSSIIGIRDMARKIEELGFESTAVNSYDNTAQVKQLAKVREIFFWKHTCVRACLSMVFMLLLYKFIPRLSPKVRSWALYQQSPIPGLFYRDIVGFMVTCYVQFWVGRHFYKAGFKSIKHGSGSMDTIVLLSTLCSFAFSLYSIANNIIKKSDKLPNVVFDASTMLIGFISVGKLLENKAKSRTNTSLSKLISLAPSTCTIIENGEPREIPVEYLQVGDVVEIKPGARLPTDGVIIQGESEIDESLITGESLMVPRYKGSPVIAGSINGPNHFLFTATSVGDDTKLSHIIQTMKQAQLNKAPIQHYADYLASKFVPTILMLALITFATWLVLSHVLSQPPAIFNDPNGKFFVCLEMTISVIVVACPCALGLAAPTAIMVGTGLGARHGVLIKGGDILEKCNTLGTFLFDKTGTLTTGHMTVHEYCAMGGSSSLSTEQLLCINAAEAVSEHPVAKALVDYTSQQLAGCSKTAIVTKSQVVLSGGLICDCELDGKSYHVVIGNKNVLQRESLPSASNNTVAYVEIDGEVIGRFEITDLIKKDAAEVVKYLQEKGHRVCMVTGDNRDSALKAAGELGIESSNVFSELTPTGKNQLVRDLQEGSGQNVAFVGDGINDSPALVTSDLGVSISTGTDIAMEAADLIILSRSDHENATLRELVYALDIAQKTFRRVKINFFWALCYNLFMLPIAMGVLVPWNITLDPIVAVACMAASSVSVVGSSLLLNGWKPPALEVSANTGNRSMWSSLAKVFRGRHDAQPDDIELQAGLIPHAASSNSLEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJJ9</accession>
    <name>C5DJJ9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0F17028p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0F17028g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554925.1">
        <property type="nucleotide sequence ID" value="XM_002554879.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJJ9"/>
    <dbReference type="FunCoup" id="C5DJJ9">
        <property type="interactions" value="54"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJJ9"/>
    <dbReference type="GeneID" id="8293158"/>
    <dbReference type="KEGG" id="lth:KLTH0F17028g"/>
    <dbReference type="eggNOG" id="ENOG502S7PB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_207409_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJJ9"/>
    <dbReference type="OMA" id="DMMAPQL"/>
    <dbReference type="OrthoDB" id="3991365at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005742">
        <property type="term" value="C:mitochondrial outer membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020266">
        <property type="entry name" value="Tom6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17112">
        <property type="entry name" value="Tom6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="57" mass="6080" checksum="D796D59CA54F9CA1" modified="2009-07-28" version="1">MSARFTAPGAGAPAKKPSRFEQFKQTPAFPVLVNLGLFVAGVAFIQSPLMDMMAPQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2023-06-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJM0</accession>
    <name>C5DJM0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0F17534p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0F17534g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554946.1">
        <property type="nucleotide sequence ID" value="XM_002554900.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJM0"/>
    <dbReference type="GeneID" id="8293181"/>
    <dbReference type="KEGG" id="lth:KLTH0F17534g"/>
    <dbReference type="HOGENOM" id="CLU_1289133_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJM0"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR24509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="214" mass="23318" checksum="3BAF1EE374EE3DA7" modified="2009-07-28" version="1">MKFAASPSAIGVSPPSLPAHPWCPLVLELELEGGTGLKVPFRPPTCHVTWSTSIVTCRRNLVIWTPIMLTMNVFHVTITVRAPLTTCSHSCLYVTITCSTLFRNPPRSRPASHVPYVLTNITPGAEPAPVVYASANQYAHNCSRSRIDAGPETKMSLSGPPELGQSRATLALVELPWPPSCEVVLCGSLPKRRPPLLTNTDIRNNVRRPPQKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJS9</accession>
    <name>C5DJS9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">malate dehydrogenase</fullName>
            <ecNumber evidence="3">1.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0F18854g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(S)-malate + NAD(+) = oxaloacetate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21432"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.37"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the LDH/MDH superfamily. MDH type 1 family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.37" evidence="3"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555005.1">
        <property type="nucleotide sequence ID" value="XM_002554959.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJS9"/>
    <dbReference type="FunCoup" id="C5DJS9">
        <property type="interactions" value="236"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJS9"/>
    <dbReference type="GeneID" id="8293241"/>
    <dbReference type="KEGG" id="lth:KLTH0F18854g"/>
    <dbReference type="eggNOG" id="KOG1494">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047181_1_0_1"/>
    <dbReference type="InParanoid" id="C5DJS9"/>
    <dbReference type="OMA" id="SGETIMP"/>
    <dbReference type="OrthoDB" id="4069699at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030060">
        <property type="term" value="F:L-malate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000268">
        <property type="entry name" value="Malate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.110.10">
        <property type="entry name" value="Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001557">
        <property type="entry name" value="L-lactate/malate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022383">
        <property type="entry name" value="Lactate/malate_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001236">
        <property type="entry name" value="Lactate/malate_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015955">
        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11540">
        <property type="entry name" value="MALATE AND LACTATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11540:SF16">
        <property type="entry name" value="MALATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02866">
        <property type="entry name" value="Ldh_1_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00056">
        <property type="entry name" value="Ldh_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000102">
        <property type="entry name" value="Lac_mal_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56327">
        <property type="entry name" value="LDH C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0520">NAD</keyword>
    <keyword evidence="5 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Lactate/malate dehydrogenase N-terminal" evidence="12">
        <location>
            <begin position="11"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="Lactate/malate dehydrogenase C-terminal" evidence="13">
        <location>
            <begin position="183"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="Lactate/malate dehydrogenase C-terminal" evidence="13">
        <location>
            <begin position="251"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="214"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="17"/>
            <end position="23"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="146"/>
            <end position="148"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR24568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="395" mass="41166" checksum="E991B8FF3E9BB62C" modified="2009-07-28" version="1">MPHHTGAQPAVKVAVLGAGGGIGQTLSLLLKTGLAPASVAQQRRVHIALYDVNRDAVAGAATDLSHIDTPVSVSWHAPEPAGADALADPLQACLAGAQLVVIPAGVPRKPGMTRDDLFNINAQIVRTLAGGIARHCDLAHVFVLLISNPVNSLVPVLVETLTQHCGADAALAAQIPRRAFGLTQLDAVRASSFLHQALDCEPSETAVVPVVGGHSGNTILPLFSQARIEPARGSGAAAESRTSTKAGAKIGAKSGATAMPALDPDVRQRLVHRVQFGGDEVVRAKNGAGSATLSMAYAGAQVACKFAEMLLGARGEVRDTLYAKVDGHVPLDAITEHVGYFSVPLTVTAAQGAAHADTHVLEQMDAYEREQLWPACLAELRESVDRGLEWAHAHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJW6</accession>
    <name>C5DJW6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 10">Histone deacetylase</fullName>
            <ecNumber evidence="2 10">3.5.1.98</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">KLTH0F19668g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>N(6)-acetyl-L-lysyl-[histone] + H2O = L-lysyl-[histone] + acetate</text>
            <dbReference type="Rhea" id="RHEA:58196"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="3.5.1.98"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9 10">Belongs to the histone deacetylase family. HD Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.98" evidence="2 10"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555042.1">
        <property type="nucleotide sequence ID" value="XM_002554996.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJW6"/>
    <dbReference type="FunCoup" id="C5DJW6">
        <property type="interactions" value="1139"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJW6"/>
    <dbReference type="GeneID" id="8293278"/>
    <dbReference type="KEGG" id="lth:KLTH0F19668g"/>
    <dbReference type="eggNOG" id="KOG1342">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007727_7_4_1"/>
    <dbReference type="InParanoid" id="C5DJW6"/>
    <dbReference type="OMA" id="GKIMEWY"/>
    <dbReference type="OrthoDB" id="1918432at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033698">
        <property type="term" value="C:Rpd3L complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070210">
        <property type="term" value="C:Rpd3L-Expanded complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032221">
        <property type="term" value="C:Rpd3S complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141221">
        <property type="term" value="F:histone deacetylase activity, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010557">
        <property type="term" value="P:positive regulation of macromolecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.800.20:FF:000001">
        <property type="entry name" value="Histone deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003084">
        <property type="entry name" value="HDAC_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF10">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037913">
        <property type="entry name" value="His_deacetylse_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01271">
        <property type="entry name" value="HISDACETLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="7 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="15">
        <location>
            <begin position="38"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="388"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="151"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="313"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00061569"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRNR" id="PIRNR037913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAR24605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="432" mass="48885" checksum="C4B98275647736E3" modified="2009-07-28" version="1">MVYQQLPFDDLKVKSTNKERVAYFYDSDVSNYSYGASHPMKPARIRMAHSLVMNYGLYKKMEIYRAKPATKQEMCQFHSDEYIDFLSRVTPDNLEMFQKESVKFNVGDDCPVFDGLYEYCSISGGGSMEGAARLNRGKCDVAINYAGGLHHAKKSEASGFCYLNDIVLGIIELLRYHPRVLYIDIDVHHGDGVEEAFYTTDRVMTCSFHKYGEFFPGTGELRDTGVGKGKYYSVNVPLRDGIDDTTYKSVFEPVIGKIMEWYQPSAVVLQCGGDSLSGDRLGCFNLSMRGHANCVNYVKSFGIPMLVVGGGGYTMRNVARTWAFETGLLNNVILDQELPYNDYYEYYGPDYELDVRPSNMFNVNTPEYLDKILTGIFSNLENTKHAPSVQINNVPRDLEDHGDVEEDTADAKDTRGGSQYARDQLIEKDNEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJZ7</accession>
    <name>C5DJZ7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0F00616p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0F00616g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554235.1">
        <property type="nucleotide sequence ID" value="XM_002554189.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJZ7"/>
    <dbReference type="GeneID" id="8292426"/>
    <dbReference type="KEGG" id="lth:KLTH0F00616g"/>
    <dbReference type="eggNOG" id="ENOG502RZ25">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063516_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJZ7"/>
    <dbReference type="OMA" id="EYETDCD"/>
    <dbReference type="OrthoDB" id="4096201at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="333" mass="37799" checksum="FE6628F764A53572" modified="2009-07-28" version="1">MELQSKRSHSALGACQEGLPLKRRVLNLAPSPVSMSSRTNIPRSSFLLSKNSTSEEDNEAYDSDFSKKPAVHFKTAPTVDSTLIDAVSKYSESEKEAPEDVGRANAGAATDPHEGLDSSYLLERRRSLSRHPSFSSSSRKQTVPRSDSVAQERCFDYLLQSIDEVWARYCNTTATAEAKVYDNLSKPQRALDPYTESHDDSFARKSSTPVTPRYQRVLSFASVNNFSDEDSDDTSGYKSEITNPTEYETDCDYRKISNLPDSIRLQSLKDRLCRAKNDLEDLHDSNSFDDCVRFWRRWDMIKYSAVEMMEEDDDDDIIESVIEELERGRCIVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>C5DK36</accession>
    <name>C5DK36_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0F01474p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0F01474g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554274.1">
        <property type="nucleotide sequence ID" value="XM_002554228.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DK36"/>
    <dbReference type="GeneID" id="8292465"/>
    <dbReference type="KEGG" id="lth:KLTH0F01474g"/>
    <dbReference type="HOGENOM" id="CLU_2622451_0_0_1"/>
    <dbReference type="InParanoid" id="C5DK36"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="78" mass="8531" checksum="5F6AF08169B1450B" modified="2009-07-28" version="1">MDSPVICVSLSDTLNACEALSEFYRDYSGNFGFQARKGFYISNDSVALVLAVRGNSNGPKAVQKKHCERSCHSKKPAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKB5</accession>
    <name>C5DKB5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0F03256p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0F03256g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554353.1">
        <property type="nucleotide sequence ID" value="XM_002554307.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKB5"/>
    <dbReference type="FunCoup" id="C5DKB5">
        <property type="interactions" value="280"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKB5"/>
    <dbReference type="GeneID" id="8292545"/>
    <dbReference type="KEGG" id="lth:KLTH0F03256g"/>
    <dbReference type="eggNOG" id="KOG0980">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004601_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKB5"/>
    <dbReference type="OMA" id="DQMRHMP"/>
    <dbReference type="OrthoDB" id="10262320at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030864">
        <property type="term" value="C:cortical actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035615">
        <property type="term" value="F:clathrin adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032051">
        <property type="term" value="F:clathrin light chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043325">
        <property type="term" value="F:phosphatidylinositol-3,4-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080025">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048268">
        <property type="term" value="P:clathrin coat assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17007">
        <property type="entry name" value="ANTH_N_Sla2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011417">
        <property type="entry name" value="ANTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030224">
        <property type="entry name" value="Sla2_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10407">
        <property type="entry name" value="HUNTINGTIN INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10407:SF15">
        <property type="entry name" value="HUNTINGTIN INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07651">
        <property type="entry name" value="ANTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="3">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="257"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="397"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="276"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR23916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="707" mass="80094" checksum="4C384C85D63FE2EF" modified="2009-07-28" version="1">MSRFDVDLDRAVRKACSYEETAPKRKHVRTCIVFTWDHKSSRAFFNAMKTQPLGEDEVSVFKALITIHKVLQEGHPSAIVEGIKNREWIESLGRIYSIGGGDGYGRIIEEYVQFLLRKLVFHRSHKGFNGTFEYEEYVSLVTTSNPDEGYETILDLMDLQDALDEFSRVLFASISSGRKNECKISALVPLVAESYGIYKFITSMIRAMHKQTGEDGAMEPLHERYVSQHARLFEFYADCSAIKYLTSLIRIPKLPTEAPSLVDSPSPEPSLSRPSTRNSARRSITPSEKPPSLKAERSFSAVTPSQTEYVATPGPQISGVFSNGAYNIPSSQPTGGVSGFVPFMSSHPTGVVGGLVPVMSSNPTGGLVGNQTGYFGNVGLDTPNSLETQRALEQQRTNQLLLQQQQQQQQQQQLEQQAILERERQRQLELQQQQRQLEIEREHQLAQQQQEQALFEERLRSQQNMYQQAAQQDQYQTDLNALREQHERDQMVIQQYDNRVLALEKELENMNLNVNEQLSNKEEQVKTMEQWKEKYDGLARLYAQLRQEHLAVLKKLKKSQQVESSAKEAIHKAEVLEHEKEDLRREVQQVNRERERLHWESEKNARKQVEEVLVAGSRAVVARTSASESATNLANCFNNFIADGANGDFAALATSIAEFTVATAGSKDAQDFLCALKDLTGTEEEMTDKVIDLNIHLQRLFANQTLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKD1</accession>
    <name>C5DKD1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0F03674g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554369.1">
        <property type="nucleotide sequence ID" value="XM_002554323.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKD1"/>
    <dbReference type="FunCoup" id="C5DKD1">
        <property type="interactions" value="906"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKD1"/>
    <dbReference type="MEROPS" id="C19.103"/>
    <dbReference type="GeneID" id="8292563"/>
    <dbReference type="KEGG" id="lth:KLTH0F03674g"/>
    <dbReference type="eggNOG" id="KOG1870">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001060_7_1_1"/>
    <dbReference type="InParanoid" id="C5DKD1"/>
    <dbReference type="OMA" id="PYCEKPE"/>
    <dbReference type="OrthoDB" id="292964at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02674">
        <property type="entry name" value="Peptidase_C19R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2230.10">
        <property type="entry name" value="DUSP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035927">
        <property type="entry name" value="DUSP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006615">
        <property type="entry name" value="Pept_C19_DUSP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF24">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06337">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00695">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143791">
        <property type="entry name" value="DUSP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51283">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="DUSP" evidence="10">
        <location>
            <begin position="72"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="9">
        <location>
            <begin position="334"/>
            <end position="1051"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="794"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1153"/>
            <end position="1198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="15"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="796"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="816"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1157"/>
            <end position="1182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR23932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1198" mass="135648" checksum="C321A87E5F5C5B57" modified="2009-07-28" version="1">MNDNLAPSNGMVADEGEKKLASVSKDDQLDIMESSSSNDQPDSVKATPPGLPDQMADQSQMAEGGAKVDAIPSLVEQRQILSEIYTQAQAESREGDAVYVIPSLWYNNFWDANITEKSRIGPIDTTSICNDFDNFLLADYNAHPYLSVPETVFKKLSEWYGLSDPSNPVKTVLIKDEDGQLVTEYNRCCFRVHFLKDSVEKYNRFSGGVRAPLFFTMSRLSSIEMVVQRCLQKFGEQEKLFDLKTHRFRIWQVQEPTSTTKSSFLATQYVLDPLTFAEMPIKTRLQSKMFGCLIKNLKLPVIDLVVELKDVTTGDHWPSNHFYYNKLLPPRGTTGLSNLGNSCYMNSALQCLVHIPELKDYFLYGGYEEDVNTQNPLGYKGLVAQAFAALIKSLFDERSSSAFAFSPREFKSIIGQQNSMFAGFLQQDSQEFLAFLLDGLHEDLNRIIEKPFVEKPELSPDEDVNNREVIHRLASKTWDKHKLRNDSVILDLFVGLYKSTLTCPVCDKTSVTFDPYSDLTLPLPVESIWTSKVRIFPNFSPPCILEVELPKGSTYQDLKNYVAEVANMNADDLIGAEIFNHAFYNNYESEGSSAKYLPIEDLISQSDVVAFYEVPRKPDDLVIPVLNSVLEQGFKTARSFGFPFFIALSETELHCYGMIRKKLEQHYTNWSGGFLNFPIISNDNEVPLDSLGLLKQKYHSVDLSKFKIDIENCSPETSVEEYFAIKVLSFRNTNNGLTENSMGNQIIWAPGHQVSLSSSQNMMTLMNNVMKDIYDYHTLAMTQKDEIEELATLSHPADDSKGHESNDEGTKKEETPSDMDVDLNENDSENFPKPDNEPEAEVDFQRPKFVKEFEAIVCEWNDMSISEVFSDEREISWDKPAELKNEKLEEAKRQRQHEGERRISLFDCLKLFSKPEVLSAADSWYCPTCREHRQATKQIQLWNSPDILTIHLKRFESRHSFSDKISDVVDFPICGLNMSDHLVCEDAEQNNLYDLVAVDNHYGGLGGGHYTAFAKNPVDGKWYYFDDSRVSETDPETSVSGAAYLLFYRRRVEGPDVGTQRLKNLLASSRQQHQLKLKEFNDRQVDFYQENHSEPEEELDAQHNFLEASEAESKEGAQENEICNVASLEVGHANSSASCEENAGRRKLRLLHKGYANSTANSSHPNSSATSSDSSDCSENISQKFYPAEGAPPTSPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKP5</accession>
    <name>C5DKP5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Histone chaperone</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0F06424g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Interacts with histone H3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the ASF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554483.1">
        <property type="nucleotide sequence ID" value="XM_002554437.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKP5"/>
    <dbReference type="FunCoup" id="C5DKP5">
        <property type="interactions" value="934"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKP5"/>
    <dbReference type="GeneID" id="8292683"/>
    <dbReference type="KEGG" id="lth:KLTH0F06424g"/>
    <dbReference type="eggNOG" id="KOG3265">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060354_0_2_1"/>
    <dbReference type="InParanoid" id="C5DKP5"/>
    <dbReference type="OMA" id="CAEPVDI"/>
    <dbReference type="OrthoDB" id="29755at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006335">
        <property type="term" value="P:DNA replication-dependent chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006337">
        <property type="term" value="P:nucleosome disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1490:FF:000001">
        <property type="entry name" value="Histone chaperone ASF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1490">
        <property type="entry name" value="Histone chaperone ASF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006818">
        <property type="entry name" value="ASF1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036747">
        <property type="entry name" value="ASF1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017282">
        <property type="entry name" value="Hist_deposition_Asf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12040">
        <property type="entry name" value="ANTI-SILENCING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12040:SF0">
        <property type="entry name" value="HISTONE CHAPERONE ASF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04729">
        <property type="entry name" value="ASF1_hist_chap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037759">
        <property type="entry name" value="Histone_Asf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101546">
        <property type="entry name" value="ASF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="151"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="156"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="199"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="229"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="265"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="276"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR037759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR24046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="296" mass="33667" checksum="7EC39042337564BE" modified="2009-07-28" version="1">MSIVSLLGINVLNNPAKFTDPYEFEITFECLEPLKNDLEWKLTYVGSSRSLEHDQELDSILVGPVPVGVNKFLFTADPPSPELIPASELVSVTVILLSCSYDGREFVRVGYYVNNEYDNEELRENPPAKVQVDQVVRNILAEKPRVTRFNIIWDNEQGEEYPPEQPELEEEEEDDEDEEQEEDDEDDNDIEEEDDEEEKAPGYESKEASESKDEKEEGPEAKKRKLKAEDEDEDEAEEIDLEEDDEEEEEEDGDAPEEDGEEEREGALEEERENTVETAPTPQDTSAAAAASDPEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKR4</accession>
    <name>C5DKR4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Tubulin alpha chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">KLTH0F06842g</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12 14">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="14">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the tubulin family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554502.1">
        <property type="nucleotide sequence ID" value="XM_002554456.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKR4"/>
    <dbReference type="FunCoup" id="C5DKR4">
        <property type="interactions" value="1390"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKR4"/>
    <dbReference type="GeneID" id="8292703"/>
    <dbReference type="KEGG" id="lth:KLTH0F06842g"/>
    <dbReference type="eggNOG" id="KOG1376">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015718_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKR4"/>
    <dbReference type="OMA" id="YMASCIL"/>
    <dbReference type="OrthoDB" id="1662883at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045298">
        <property type="term" value="C:tubulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045143">
        <property type="term" value="P:homologous chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000070">
        <property type="term" value="P:mitotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030473">
        <property type="term" value="P:nuclear migration along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02186">
        <property type="entry name" value="alpha_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000005">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000001">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000008">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002452">
        <property type="entry name" value="Alpha_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01162">
        <property type="entry name" value="ALPHATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10 14" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 14" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="15">
        <location>
            <begin position="50"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="16">
        <location>
            <begin position="249"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAR24065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="447" mass="49721" checksum="5533FEEFBB05CC3E" modified="2009-07-28" version="1">MREVISVNVGQAGCQIGNACWELYSLEHGIRPDGYLEEGLSKPRGGEEGFSTFFNETGSGKFVPRAVYVDLEPNVIDEVRTGPYKDLFHPEQLISGKEDAANNYARGHYTVGRELLDDILDRIRKISDQCDGLQGFLFTHSLGGGTGSGLGSLLLEQLSIDYGKKSKLEFAVYPAPQVSTSVVEPYNTVLTTHTTLEHADCTFMVDNEAIYDMCKKNLDISRPSFSNLNNLIAQVVSSVTASLRFDGSLNVDLNEFQTNLVPYPRIHFPLVSYAPIQSKSKAHHESNAVSEITNACFEPGNQMVKCDPRTGKYMATCLLYRGDVVTRDVQNAVAQVKNKRTVQLVDWCPTGFKIGICYEPPTATPQSQLSSVNRAVCMLSNTTAIADAWKRIDRKFDLMYAKRAFVHWYVGEGMEEGEFTEAREDLAALERDYIEVGADSYADEEEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKU5</accession>
    <name>C5DKU5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ER membrane protein complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0F07612g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the ER membrane protein complex (EMC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the EMC2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554533.1">
        <property type="nucleotide sequence ID" value="XM_002554487.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKU5"/>
    <dbReference type="FunCoup" id="C5DKU5">
        <property type="interactions" value="197"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKU5"/>
    <dbReference type="GeneID" id="8292738"/>
    <dbReference type="KEGG" id="lth:KLTH0F07612g"/>
    <dbReference type="eggNOG" id="KOG3060">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065213_1_0_1"/>
    <dbReference type="InParanoid" id="C5DKU5"/>
    <dbReference type="OMA" id="LMEMLFY"/>
    <dbReference type="OrthoDB" id="124397at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039856">
        <property type="entry name" value="EMC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12760">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="159"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU367091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR24096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="284" mass="32431" checksum="14AD9D2529F0ED6B" modified="2009-07-28" version="1">MEILKDRYLTICSTKYYTAAPAHEVASLWDQLKTYLGAGDPTLSELDYMSLTHMLFDLSIYNGKDVEAETLFKTFRDRFGSSSPYLHVMQATLLQVNKGDREAEEFLTKLLKDALEYETDIIDYLLVSKKLLSIRRKSITKEEYLKQLLGLAEKFPVDGELWYAIAQEYASLGQFEQAAYSLEEVLCIAPFNYVAFAQLSEALYYRALREKKDKDSLLQQSLSNALRSVELSENFVKGWAFVAVTSKELSKAKLLNLSKKKLREIMVGCNDTDKATAKKILENL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKV7</accession>
    <name>C5DKV7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">T-complex protein 1 subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">CCT-alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0F07876g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554545.1">
        <property type="nucleotide sequence ID" value="XM_002554499.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKV7"/>
    <dbReference type="FunCoup" id="C5DKV7">
        <property type="interactions" value="1457"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKV7"/>
    <dbReference type="GeneID" id="8292750"/>
    <dbReference type="KEGG" id="lth:KLTH0F07876g"/>
    <dbReference type="eggNOG" id="KOG0360">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008891_5_1_1"/>
    <dbReference type="InParanoid" id="C5DKV7"/>
    <dbReference type="OMA" id="RGPNDYQ"/>
    <dbReference type="OrthoDB" id="10248520at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03335">
        <property type="entry name" value="TCP1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000009">
        <property type="entry name" value="T-complex protein 1 subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000070">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012715">
        <property type="entry name" value="Chap_CCT_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02340">
        <property type="entry name" value="chap_CCT_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 10" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR24108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="559" mass="60293" checksum="A7EFD6CEEB7E142D" modified="2009-07-28" version="1">MSSVLNNSRSDTLFLGGEKISGDDIRHQNVLAALAAANVVKSSLGPVGLDKMLVDDIGDFTVTNDGATILSLLDVQHPAGKILVELAQQQDREIGDGTTSVVIIASELLKRANSLVKNKIHPTTIITGFRVALREAIRYINEVLSIPVESLGKESMVNIAKTSMSSKIIGSDSDFFSNMVVDALLAVKTQNSKGEIKYPVKAVNILKAHGKSARESVLVQGYALNCTVASQAMPKRLDGGNIKIACLDINLQKARMAMGVQININDPEQLEEIRKREAGIVLERVQKIIAAGANVVLTTKGIDDLCLKEFVEAKVMAVRRCKKEDLRRIARATGATLISSMSNLEGDETFEPSSLGTCQEVVQAKFSDDECILIKGTSKHSSSSIVLRGANDYSLDEMERSIHDSLSVVKRTLESGNVVPGGGCVEAALNIYLDNFATTVGSREQLAIAEFAASLLVIPKTLAVNAAKDSSDLIAKLRSYHAASQQAQLDDTKRRNYRNYGLDLIKGKVVDEVHSGVLEPTISKVKSLKSALEACVAILRIDTMITVDPEPPKEDPHAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>C5DL07</accession>
    <name>C5DL07_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Small ribosomal subunit protein mS35</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">37S ribosomal protein S24, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0F08998g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the mitochondrion-specific ribosomal protein mS35 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554595.1">
        <property type="nucleotide sequence ID" value="XM_002554549.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DL07"/>
    <dbReference type="FunCoup" id="C5DL07">
        <property type="interactions" value="171"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DL07"/>
    <dbReference type="GeneID" id="8292801"/>
    <dbReference type="KEGG" id="lth:KLTH0F08998g"/>
    <dbReference type="eggNOG" id="KOG3933">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_943997_0_0_1"/>
    <dbReference type="InParanoid" id="C5DL07"/>
    <dbReference type="OMA" id="YRQEYKP"/>
    <dbReference type="OrthoDB" id="283424at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017081">
        <property type="entry name" value="Ribosomal_mS35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019349">
        <property type="entry name" value="Ribosomal_mS35_mit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039848">
        <property type="entry name" value="Ribosomal_mS35_mt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13490">
        <property type="entry name" value="MITOCHONDRIAL 28S RIBOSOMAL PROTEIN S28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13490:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10213">
        <property type="entry name" value="MRP-S28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036995">
        <property type="entry name" value="RSM24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein mS35 mitochondrial conserved" evidence="2">
        <location>
            <begin position="154"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR036995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR24158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="301" mass="34882" checksum="D4FB5BB161741521" modified="2009-07-28" version="1">MRCGIPLMIRSALICRNSVTNSAVKTGSIATELYLEPSKWRALKPEQIFSLYQERVVRLGSEYKPCKEELDALLSTAETTGVSPKTIRNIYYGGEQAAYDATGANIEDDFKPSPFMFDDLPSQAQALVQQHREQRFYNRLAAYELPKLVQYRQEYKRPSQKTHPVTYRYTTYIGEEHPNSRKVVVSLKTQQLGLNDKEVHKFRLLARTRYDHTTDIFKMSSDRYPEAAQNARYLSDKLQALIKEAKDTSDDFSDVPLDTRHTTARNLRRKTRGYKFPEEWKRPQDAPKEMVNVAELIQKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DL69</accession>
    <name>C5DL69_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription and mRNA export factor SUS1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">SUS1</name>
        <name evidence="4" type="ordered locus">KLTH0F10450g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in mRNA export coupled transcription activation by association with both the TREX-2 and the SAGA complexes. At the promoters, SAGA is required for recruitment of the basal transcription machinery. It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction and promoter selectivity, interaction with transcription activators, and chromatin modification through histone acetylation and deubiquitination. Within the SAGA complex, participates to a subcomplex required for deubiquitination of H2B and for the maintenance of steady-state H3 methylation levels. The TREX-2 complex functions in docking export-competent ribonucleoprotein particles (mRNPs) to the nuclear entrance of the nuclear pore complex (nuclear basket). TREX-2 participates in mRNA export and accurate chromatin positioning in the nucleus by tethering genes to the nuclear periphery. May also be involved in cytoplasmic mRNA decay by interaction with components of P-bodies.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the nuclear pore complex (NPC)-associated TREX-2 complex (transcription and export complex 2), composed of at least SUS1, SAC3, THP1, SEM1, and CDC31. TREX-2 contains 2 SUS1 chains. The TREX-2 complex interacts with the nucleoporin NUP1. Component of the 1.8 MDa SAGA transcription coactivator-HAT complex. SAGA is built of 5 distinct domains with specialized functions. Within the SAGA complex, SUS1, SGF11, SGF73 and UBP8 form an additional subcomplex of SAGA called the DUB module (deubiquitination module). Interacts directly with THP1, SAC3, SGF11, and with the RNA polymerase II.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">P-body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ENY2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554657.1">
        <property type="nucleotide sequence ID" value="XM_002554611.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DL69"/>
    <dbReference type="FunCoup" id="C5DL69">
        <property type="interactions" value="381"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DL69"/>
    <dbReference type="GeneID" id="8292867"/>
    <dbReference type="KEGG" id="lth:KLTH0F10450g"/>
    <dbReference type="eggNOG" id="KOG4479">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134052_2_1_1"/>
    <dbReference type="InParanoid" id="C5DL69"/>
    <dbReference type="OMA" id="YESGWFD"/>
    <dbReference type="OrthoDB" id="6221744at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071819">
        <property type="term" value="C:DUBm complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070390">
        <property type="term" value="C:transcription export complex 2"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03046">
        <property type="entry name" value="ENY2_Sus1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018783">
        <property type="entry name" value="TF_ENY2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038212">
        <property type="entry name" value="TF_EnY2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12514">
        <property type="entry name" value="ENHANCER OF YELLOW 2 TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10163">
        <property type="entry name" value="EnY2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0010">Activator</keyword>
    <keyword evidence="1 3" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2 3" id="KW-0811">Translocation</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR24220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="99" mass="11187" checksum="96B1D3ADEE3CE558" modified="2009-07-28" version="1">MTANGAKTMELKAQIQQYLVESGNYELISNKLSRKLLEEGWTDQVKRMTSEEIRADDSASFTQILSKVEPKALGMVSDSTKDEILSQIRNFLSEIVETE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLC0</accession>
    <name>C5DLC0_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Elongation factor 1-alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0F11726g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554708.1">
        <property type="nucleotide sequence ID" value="XM_002554662.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLC0"/>
    <dbReference type="FunCoup" id="C5DLC0">
        <property type="interactions" value="2066"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLC0"/>
    <dbReference type="GeneID" id="8292921"/>
    <dbReference type="KEGG" id="lth:KLTH0F11726g"/>
    <dbReference type="eggNOG" id="KOG0052">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007265_3_5_1"/>
    <dbReference type="InParanoid" id="C5DLC0"/>
    <dbReference type="OMA" id="AIRDMGM"/>
    <dbReference type="OrthoDB" id="3969558at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01883">
        <property type="entry name" value="EF1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03693">
        <property type="entry name" value="EF1_alpha_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03705">
        <property type="entry name" value="EF1_alpha_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000003">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000005">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000211">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00118_A">
        <property type="entry name" value="EF_Tu_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054696">
        <property type="entry name" value="GTP-eEF1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050100">
        <property type="entry name" value="TRAFAC_GTPase_members"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004539">
        <property type="entry name" value="Transl_elong_EF1A_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00483">
        <property type="entry name" value="EF-1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008969">
        <property type="entry name" value="PRK12317.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23115">
        <property type="entry name" value="TRANSLATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22594">
        <property type="entry name" value="GTP-eEF1A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="8 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="10">
        <location>
            <begin position="5"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR24271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="458" mass="49876" checksum="9340138399928F20" modified="2009-07-28" version="1">MGKEKSHVNVVVIGHVDSGKSTTTGHLIFKCGGIDKRTIEKFEKEAAELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYHVTVIDAPGHRDFIKNMITGTSQADCAILIIAGGVGEFEAGISKDGQTREHALLAYTLGVKQLIVAINKMDSVKWDESRYQEIVKETSNFIKKVGYNPKNVPFVPISGWNGDNMIEATTNAPWYKGWEKETKAGTVKGKTLLEAIDAIEPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMVVTFAPAGVTTEVKSVEMHHEQLEAGVPGDNVGFNVKNVSVKEIRRGNVCGDSKNDPPKAAASFNAQVIVLNHPGQISAGYSPVLDCHTAHIACRFDELLVKIDRRTGKTLEEAPKFIKSGDAAMVKFVPSKPMCVEAFTDYPPLGRFAVRDMRQTVAVGVIKSVDKSDKAGKVTKAAQKAAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>C5DM46</accession>
    <name>C5DM46_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="7">PPIase</shortName>
            <ecNumber evidence="7">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0G05918g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 7">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the cyclophilin-type PPIase family. PPIase B subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="7"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555294.1">
        <property type="nucleotide sequence ID" value="XM_002555248.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DM46"/>
    <dbReference type="FunCoup" id="C5DM46">
        <property type="interactions" value="263"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DM46"/>
    <dbReference type="GeneID" id="8293562"/>
    <dbReference type="KEGG" id="lth:KLTH0G05918g"/>
    <dbReference type="eggNOG" id="KOG0880">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012062_4_2_1"/>
    <dbReference type="InParanoid" id="C5DM46"/>
    <dbReference type="OMA" id="ENHEITH"/>
    <dbReference type="OrthoDB" id="193499at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016018">
        <property type="term" value="F:cyclosporin A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000019">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024936">
        <property type="entry name" value="Cyclophilin-type_PPIase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF561">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001467">
        <property type="entry name" value="Peptidylpro_ismrse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0697">Rotamase</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5006523008" description="Peptidyl-prolyl cis-trans isomerase" evidence="7">
        <location>
            <begin position="20"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="8">
        <location>
            <begin position="30"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR24857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="208" mass="22992" checksum="306EEFE36B2E150F" modified="2009-07-28" version="1">MLALRLIASVFAFFVCVRAASDAVVTHKVYFDIQHGDKNVGRIVLGLFGNVAPKTVQNFYELSTSEDPSMGYLNSTFHRIISNFMIQGGDFTDHSGIGGKSIYGNTFEDESFELKHDKPGRLSMANRGRNTNGSQFFITTVPTPWLDGRHVVFGEVIDGMDVVEYLDSVPTGRGDKPKEVVKIVACGEVETVPVREQLKEEAGIRDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DM69</accession>
    <name>C5DM69_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0G06424p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0G06424g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555317.1">
        <property type="nucleotide sequence ID" value="XM_002555271.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DM69"/>
    <dbReference type="FunCoup" id="C5DM69">
        <property type="interactions" value="62"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DM69"/>
    <dbReference type="GeneID" id="8293585"/>
    <dbReference type="KEGG" id="lth:KLTH0G06424g"/>
    <dbReference type="eggNOG" id="KOG2669">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053395_0_0_1"/>
    <dbReference type="InParanoid" id="C5DM69"/>
    <dbReference type="OMA" id="HYELDIE"/>
    <dbReference type="OrthoDB" id="10069473at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099122">
        <property type="term" value="F:RNA polymerase II C-terminal domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17003">
        <property type="entry name" value="CID_Rtt103"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006569">
        <property type="entry name" value="CID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047883">
        <property type="entry name" value="Rtt103-like_CID"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12460:SF0">
        <property type="entry name" value="CID DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12460">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE INHIBITOR-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04818">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00582">
        <property type="entry name" value="RPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51391">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CID" evidence="2">
        <location>
            <begin position="1"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="251"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="277"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="300"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR24880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="353" mass="39665" checksum="24A4DD6B216C5A77" modified="2009-07-28" version="1">MSFSQEQLTSKLNALDETQESIVSASKWLLSQYKEASQVAQCWRQFILKSSTNTRRKLLAIYLANDVIQQSKHKRVGEFGTAFGAIMPEVLGKVYPEFTQELQRKVKRVVDIWRERQVFSDSVFKELYPQLKQSKRPTEPRALPLDIAPEIKRLVEVFAKLTKCQSSTFAAKTRFDTSLEALDPNSVVYLENYKTVQKVGQAAKDSLDKSVSYRKQAIKELQIMLNFQEEQMKQDQSLISEIDQVLISKDPASATSDPLGETDLLPTYEASDGSDSDSDRSIGGEGEPPRKRQSLGTDSNELKRAKTETGTIDEVRTEEYETEAYEPTPAEVAANSSLAVTSSIQDLLSKLAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMC9</accession>
    <name>C5DMC9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA(adenine(34)) deaminase</fullName>
            <ecNumber evidence="3">3.5.4.33</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0G07832g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>adenosine(34) in tRNA + H2O + H(+) = inosine(34) in tRNA + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:43168"/>
            <dbReference type="Rhea" id="RHEA-COMP:10373"/>
            <dbReference type="Rhea" id="RHEA-COMP:10374"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:82852"/>
            <dbReference type="EC" id="3.5.4.33"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytidine and deoxycytidylate deaminase family. ADAT2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.33" evidence="3"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555377.1">
        <property type="nucleotide sequence ID" value="XM_002555331.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMC9"/>
    <dbReference type="FunCoup" id="C5DMC9">
        <property type="interactions" value="554"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMC9"/>
    <dbReference type="GeneID" id="8293648"/>
    <dbReference type="KEGG" id="lth:KLTH0G07832g"/>
    <dbReference type="eggNOG" id="KOG1018">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025810_8_1_1"/>
    <dbReference type="InParanoid" id="C5DMC9"/>
    <dbReference type="OMA" id="PCQMCAG"/>
    <dbReference type="OrthoDB" id="1701769at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052718">
        <property type="term" value="C:tRNA-specific adenosine-34 deaminase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052717">
        <property type="term" value="F:tRNA-specific adenosine-34 deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002100">
        <property type="term" value="P:tRNA wobble adenosine to inosine editing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01285">
        <property type="entry name" value="nucleoside_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000039">
        <property type="entry name" value="tRNA-specific adenosine deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016192">
        <property type="entry name" value="APOBEC/CMP_deaminase_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11079">
        <property type="entry name" value="CYTOSINE DEAMINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11079:SF149">
        <property type="entry name" value="TRNA-SPECIFIC ADENOSINE DEAMINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00383">
        <property type="entry name" value="dCMP_cyt_deam_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00903">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="9">
        <location>
            <begin position="4"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR24940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="250" mass="28382" checksum="EABADB71DE4A2E8F" modified="2009-07-28" version="1">MSLDSYVHFMRMATKLARYALDHGETPVACIFVHTPADQVVAYGMNDTNRSLTGIAHAEFMGIEQIQSKFGAQDTSIFKDITLYVTVEPCIMCASALKQLGIQKVVFGCGNERFGGNGSILSIQKDSCTAPQNKHISVPGILRKEAIMLLRYFYVRENERSPKPKAKANRKLDVSTFPPIDWSLYLTREEFKDILGSEYLSHYDEKLDLSKTVDWGLIDGNYDDFFLNIKQQCDQFSLQVPKKLKQENCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5DME9</accession>
    <name>C5DME9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0G08294p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0G08294g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555397.1">
        <property type="nucleotide sequence ID" value="XM_002555351.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DME9"/>
    <dbReference type="FunCoup" id="C5DME9">
        <property type="interactions" value="155"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DME9"/>
    <dbReference type="GeneID" id="8293669"/>
    <dbReference type="KEGG" id="lth:KLTH0G08294g"/>
    <dbReference type="eggNOG" id="KOG0393">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041217_21_2_1"/>
    <dbReference type="InParanoid" id="C5DME9"/>
    <dbReference type="OMA" id="NDNVMRR"/>
    <dbReference type="OrthoDB" id="8830751at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007163">
        <property type="term" value="P:establishment or maintenance of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000573">
        <property type="entry name" value="RHO family GTPase Rho2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0636">Prenylation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR24960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="187" mass="20860" checksum="E7D1EDBE8E318F0D" modified="2009-07-28" version="1">MTENVVRRKLVIIGDGACGKTSLLYVFALGKFPKEYHPTVFENYVTDCRVDGIKVSLALWDTAGQEEYERLRPFSYSKANVILIGFAIDDAESLLNARTKWTEEALRYCPEAPIVLVGLKRDLRKSESSHKFVTREEAEQVARAIGAKKYMECSALDGSGVDDVFELATRTSLLVNKEPGQTCCLIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMH1</accession>
    <name>C5DMH1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">U3 small nucleolar ribonucleoprotein protein IMP4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0G08866g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555419.1">
        <property type="nucleotide sequence ID" value="XM_002555373.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMH1"/>
    <dbReference type="FunCoup" id="C5DMH1">
        <property type="interactions" value="975"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMH1"/>
    <dbReference type="GeneID" id="8293695"/>
    <dbReference type="KEGG" id="lth:KLTH0G08866g"/>
    <dbReference type="eggNOG" id="KOG2781">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040063_2_0_1"/>
    <dbReference type="InParanoid" id="C5DMH1"/>
    <dbReference type="OMA" id="IGTMSEQ"/>
    <dbReference type="OrthoDB" id="10253204at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034457">
        <property type="term" value="C:Mpp10 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042134">
        <property type="term" value="F:rRNA primary transcript binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10480:FF:000001">
        <property type="entry name" value="IMP4, U3 small nucleolar ribonucleoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10480">
        <property type="entry name" value="Probable brix-domain ribosomal biogenesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007109">
        <property type="entry name" value="Brix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044281">
        <property type="entry name" value="IMP4/RPF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22734">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RIBONUCLEOPROTEIN PROTEIN IMP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22734:SF2">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RIBONUCLEOPROTEIN PROTEIN IMP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04427">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00879">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50833">
        <property type="entry name" value="BRIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="Brix" evidence="3">
        <location>
            <begin position="79"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00040513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR24982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="283" mass="32637" checksum="47F16DBE3BDA338B" modified="2009-07-28" version="1">MLRKQARERREYLYRKAQELQDSQLQQKRQLIKHALAQGKPLPKDVAEDEQLQKDFQYDQTIQETIDDEYSATSGLAEPKVIVTTSRDPSTRLSQFAKEIRLLFPSAVRLNRGNYIMPNLVDACKKSGTSDLVVLHEHRGVPTSLTISHFPHGPTAYFTLHNVVLRHDILNAGNQSEVNPHLIFDNFTTPLGQRVVTILKHIFPPGPKKDSPRVITFANRGDFISVRQHVYVRTREGVELAEVGPRFEMRLFELRLGTLDNKEADVEWQLRRFVRTASRKDYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMJ3</accession>
    <name>C5DMJ3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">peptide chain release factor N(5)-glutamine methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.297</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0G09416g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-glutaminyl-[peptide chain release factor] + S-adenosyl-L-methionine = N(5)-methyl-L-glutaminyl-[peptide chain release factor] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42896"/>
            <dbReference type="Rhea" id="RHEA-COMP:10271"/>
            <dbReference type="Rhea" id="RHEA-COMP:10272"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30011"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61891"/>
            <dbReference type="EC" id="2.1.1.297"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.1.1.297" evidence="1"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555441.1">
        <property type="nucleotide sequence ID" value="XM_002555395.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMJ3"/>
    <dbReference type="FunCoup" id="C5DMJ3">
        <property type="interactions" value="95"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMJ3"/>
    <dbReference type="GeneID" id="8293719"/>
    <dbReference type="KEGG" id="lth:KLTH0G09416g"/>
    <dbReference type="eggNOG" id="KOG2904">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018398_0_2_1"/>
    <dbReference type="InParanoid" id="C5DMJ3"/>
    <dbReference type="OMA" id="MPRIPYS"/>
    <dbReference type="OrthoDB" id="269872at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102559">
        <property type="term" value="F:peptide chain release factor N(5)-glutamine methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004556">
        <property type="entry name" value="HemK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050320">
        <property type="entry name" value="N5-glutamine_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007848">
        <property type="entry name" value="Small_mtfrase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00536">
        <property type="entry name" value="hemK_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18895">
        <property type="entry name" value="HEMK METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18895:SF74">
        <property type="entry name" value="MTRF1L RELEASE FACTOR GLUTAMINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05175">
        <property type="entry name" value="MTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase small" evidence="6">
        <location>
            <begin position="94"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR25004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="293" mass="33599" checksum="16565EEDB6F35A50" modified="2009-07-28" version="1">MVRIKPSVLKKACQINPLLPLLLPECRTIEAAQQELRWIQNEVSDKKRVRQCCMLRARHYPLQYLLGSQPFGPLDVTCQPGVLIPRWETEEWTVSLAEKLPRDMAFDIMDLCTGTGCIPLLLKQMCPKCSVFAVDRSPLAYKLATRNSNTLQVPLKIIKKDILERSSEIVPRTVDLITCNPPYIPRTTFARETARSVKLFEPKLALLGNKEFYANLVDCWMSRTDAFVYEVGDLSQCYYVLNRVTGDVDLSRKWRIGFRHDSCGRPRIVYGFRASGSRLNWASILVKFGDMKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMJ9</accession>
    <name>C5DMJ9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">GTP-binding protein RHO3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0G09548g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555447.1">
        <property type="nucleotide sequence ID" value="XM_002555401.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMJ9"/>
    <dbReference type="FunCoup" id="C5DMJ9">
        <property type="interactions" value="167"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMJ9"/>
    <dbReference type="GeneID" id="8293725"/>
    <dbReference type="KEGG" id="lth:KLTH0G09548g"/>
    <dbReference type="eggNOG" id="KOG0393">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041217_21_2_1"/>
    <dbReference type="InParanoid" id="C5DMJ9"/>
    <dbReference type="OMA" id="THTIMLC"/>
    <dbReference type="OrthoDB" id="8830751at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007163">
        <property type="term" value="P:establishment or maintenance of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017157">
        <property type="term" value="P:regulation of exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000780">
        <property type="entry name" value="Rho GTPase Rho3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0636">Prenylation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="211"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="213"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00067968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR25010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="231" mass="25376" checksum="F640BC74B1586985" modified="2009-07-28" version="1">MPICGGSSASKKPIERKIVILGDGACGKTSLLNVFTRGYFPKVYEPTVFENYIHDIFVDSQHITLSLWDTAGQEEFDRLRSLSYSDTHTIMLCFSVDSRDSLDNVQHKWVGEIADHCEGVKLVLVALKCDLRSNEEEFALDTAITPGNIQQQQQQQQQSSVQSGGLISYEEGLAMAKKIGALRYLECSAKMNRGVNEAFTEAARCALTATPKGAKDHAGEESKKGSSCTVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMU7</accession>
    <name>C5DMU7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="15">KLTH0G11748p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0G11748g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the fungal Na(+)/H(+) exchanger family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555545.1">
        <property type="nucleotide sequence ID" value="XM_002555499.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMU7"/>
    <dbReference type="FunCoup" id="C5DMU7">
        <property type="interactions" value="53"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMU7"/>
    <dbReference type="GeneID" id="8293824"/>
    <dbReference type="KEGG" id="lth:KLTH0G11748g"/>
    <dbReference type="eggNOG" id="KOG4505">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008635_0_1_1"/>
    <dbReference type="InParanoid" id="C5DMU7"/>
    <dbReference type="OMA" id="TNENIGT"/>
    <dbReference type="OrthoDB" id="5327978at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015385">
        <property type="term" value="F:sodium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120029">
        <property type="term" value="P:proton export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036376">
        <property type="term" value="P:sodium ion export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1530.20:FF:000015">
        <property type="entry name" value="Na(+)/H(+) antiporter 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013928">
        <property type="entry name" value="Cation/H_antiporter_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004712">
        <property type="entry name" value="Na+/H+_antiporter_fungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31382">
        <property type="entry name" value="NA(+)/H(+) ANTIPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31382:SF4">
        <property type="entry name" value="NA(+)_H(+) ANTIPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08619">
        <property type="entry name" value="Nha1_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0050">Antiport</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="10" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="14"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="75"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="103"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="204"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="245"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="296"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="327"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="361"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="407"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="13">
        <location>
            <begin position="25"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="Alkali metal cation/H+ antiporter Nha1 C-terminal" evidence="14">
        <location>
            <begin position="463"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="domain" description="Alkali metal cation/H+ antiporter Nha1 C-terminal" evidence="14">
        <location>
            <begin position="872"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="485"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="581"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="623"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="717"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="810"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="510"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="543"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="585"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="645"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="717"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="836"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="883"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="965"/>
            <end position="979"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR25108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="990" mass="108953" checksum="28A69CFD205C93AB" modified="2009-07-28" version="1">MAVWSHLEVTKSHVAYACVGVFSAIFSLVSLFAKEKLYIGESTVAVVFGLIVGPHCLDWFNPIEWGNTDSITLEITRIVLCLQIFAVAVELPKKYMLKHWLSVTMLLLPVMTAGWLILALFVWIVIPHVSFPEALLVASCVTATDPILAQSVVSGKFAQRVPGHLRNLLTAESGCNDGMAFPFIYLSLNLLFHSGHGGEIVKDWICVTILYECLFGCILGAFIGYTGRRAIKFAEEKKLIDRESFLAFYVVLAFICAGFGSMLSVDDLLVSFAAGTAFAWDGWFSKKTKESEISTIIDLLLNLAYFVYFGAIIPWQQFNDPEIGTNAWRLVILAIVVIVLRRIPAVLAFKAFIPDIKSWREALFVGHFGPIGVGAVFAAILARSELEGHATGEETPLKQLPEKGSEYWRLIALIWPMTCFFIVVSIIVHGSSVAVITLGRHLNTITLTKSFTTHTTNGKGNSWMQRLPSLDKSGRSFSLQRIDTKASPETLARQETVETSGVPAKPAGGMKRKRRKLRNRIKKDLFRSRTNNGGNGEDSENLDSERLQREKEAQAAAFALSAELHHDDDASDATDIDAEATGTDAETEEGTIEEPLELVDVMDPSEASHTINRLDAISHGEKVDTESLGHQSQKSKPEPSPSNSLEGQSPNQGTTTTSSSNFDEEKLDRESQAASAASMDSYARDLEKLRKDMEAKRDEEEGAVAYTEGNKIIVENKDGEIIDQAELNRKPRDEEEGPDSTTPQDRKSRSRSYSINSLKRALTPPRMRRQTLVDEQANKRYFAYKIDDQLIIENDDGDVLRRYKINPHAEAGAKPAPKRKPTGVVAKALSAVGLSKGSSRAPSENTSGSSAQGDVAQPQSSVAKIRDRRLTPVPTSGAMDVGSSEDEEEESEEDGSSDEESERAIDDEPESASDYDGSEDGSYTDEGFSDEEPETAIERARRLTALGHVSAPRREDDEEFVPGNRETQNAESSGPSNKVKSALSRPFRKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DN52</accession>
    <name>C5DN52_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">KLTH0G14234p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0G14234g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Monothiol glutaredoxin involved in the biogenesis of iron-sulfur clusters. Binds one iron-sulfur cluster per dimer. The iron-sulfur cluster is bound between subunits, and is complexed by a bound glutathione and a cysteine residue from each subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer. Heterodimer with FRA2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutaredoxin family. Monothiol subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555650.1">
        <property type="nucleotide sequence ID" value="XM_002555604.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DN52"/>
    <dbReference type="FunCoup" id="C5DN52">
        <property type="interactions" value="920"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DN52"/>
    <dbReference type="GeneID" id="8293935"/>
    <dbReference type="KEGG" id="lth:KLTH0G14234g"/>
    <dbReference type="eggNOG" id="KOG0911">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026126_12_0_1"/>
    <dbReference type="InParanoid" id="C5DN52"/>
    <dbReference type="OMA" id="WAEPCKT"/>
    <dbReference type="OrthoDB" id="415696at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990229">
        <property type="term" value="C:iron-sulfur cluster assembly complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015036">
        <property type="term" value="F:disulfide oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061629">
        <property type="term" value="F:RNA polymerase II-specific DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03028">
        <property type="entry name" value="GRX_PICOT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02984">
        <property type="entry name" value="TRX_PICOT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000012">
        <property type="entry name" value="Monothiol glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000092">
        <property type="entry name" value="Monothiol glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033658">
        <property type="entry name" value="GRX_PICOT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004480">
        <property type="entry name" value="Monothiol_GRX-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10293">
        <property type="entry name" value="GLUTAREDOXIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10293:SF73">
        <property type="entry name" value="GLUTAREDOXIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="10">
        <location>
            <begin position="3"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="113"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="115"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00055846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00065077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR25213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="240" mass="26989" checksum="7365A44932879F40" modified="2009-07-28" version="1">MVVIEITSQDQFTDLTTVNAEGKLIALYFHTTWAEPCKTMNTVYTALSEEPVNKEVQFLAINADDNAEIAELFEVSSVPYFVLVRNNTILKELSGADPKEFVKALNQFNGSLAEGAGAPPQPAQNEGVEEEEPVETEEELMERLKKLTQAAPVMLFMKGTPSEPKCGFSRQMVGILREYQVRFGFFDILKDDSIRQGLKKFSDWPTFPQLYISGEFQGGLDIIKESLEEDPEFFQHAMNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DN83</accession>
    <name>C5DN83_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0G14916p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0G14916g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555681.1">
        <property type="nucleotide sequence ID" value="XM_002555635.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DN83"/>
    <dbReference type="FunCoup" id="C5DN83">
        <property type="interactions" value="117"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DN83"/>
    <dbReference type="GeneID" id="8293966"/>
    <dbReference type="KEGG" id="lth:KLTH0G14916g"/>
    <dbReference type="eggNOG" id="KOG4288">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055314_1_0_1"/>
    <dbReference type="InParanoid" id="C5DN83"/>
    <dbReference type="OMA" id="WERADIF"/>
    <dbReference type="OrthoDB" id="276721at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051207">
        <property type="entry name" value="ComplexI_NDUFA9_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12126:SF16">
        <property type="entry name" value="MIOREX COMPLEX COMPONENT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12126">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 39 KDA SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="1">
        <location>
            <begin position="5"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR25244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="269" mass="30740" checksum="96753A7F82AD9860" modified="2009-07-28" version="1">MSSLLVFGGNGFLGRRICQTAVEKGFQVTSLSRSGSPPRTANRWDKEWIDKVHWEKCDVLDPKSYTQYLKDADNVVHSIGILLEDSSYKAQINGKLSFDPKNLLKWGPNPMKNNPNFTYEVMNKKTALMLAEEFSKVQRADSARERTMSYISADRGFPGLPSGYIKSKREAEAGIMRHEQQFRPILVRPGFMFDELDASLKTLDVRSQLKHVLEMLNWGNDWLLGRRIDFINQLIRPTVSTQQVSRALLQKIESPDFKGVLTLEEIIKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DN91</accession>
    <name>C5DN91_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein N-terminal and lysine N-methyltransferase EFM7</fullName>
            <ecNumber evidence="5">2.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Elongation factor methyltransferase 7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">EFM7</name>
        <name evidence="6" type="ordered locus">KLTH0G15092g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">S-adenosyl-L-methionine-dependent protein methyltransferase that trimethylates the N-terminal glycine 'Gly-2' of elongation factor 1-alpha, before also catalyzing the mono- and dimethylation of 'Lys-3'.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the class I-like SAM-binding methyltransferase superfamily. EFM7 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="5"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DN91"/>
    <dbReference type="FunCoup" id="C5DN91">
        <property type="interactions" value="159"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DN91"/>
    <dbReference type="KEGG" id="lth:KLTH0G15092g"/>
    <dbReference type="eggNOG" id="KOG2920">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032409_0_0_1"/>
    <dbReference type="InParanoid" id="C5DN91"/>
    <dbReference type="OMA" id="VGHNPLW"/>
    <dbReference type="OrthoDB" id="46564at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071885">
        <property type="term" value="F:N-terminal protein N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03223">
        <property type="entry name" value="Methyltr_EFM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025784">
        <property type="entry name" value="EFM7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614:SF10">
        <property type="entry name" value="PROTEIN N-TERMINAL AND LYSINE N-METHYLTRANSFERASE EFM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10294">
        <property type="entry name" value="Methyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51560">
        <property type="entry name" value="SAM_MT_NNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="5">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="89"/>
            <end position="91"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR25252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="266" mass="29835" checksum="F4B49CC48B7674A3" modified="2009-07-28" version="1">MSDTESFIQGDIFAEPEGYFQAEPEAHFAEYVREQVPSASKSQKKEVRLRLVGSSPLWGHLLWNAGIYTANHLDKFPDLVKDRCVLELGAAGALPSVVAGMIGAKKCVVTDYPDADLLQNIQYNVDHEVFDGEALPSDGAGASERNVVVEGYIWGNDYAPLVSHLPCGTTKFDLVILSDLVFNHTEHLKLLQTTKDLLAQDGKALVVFSPHRPWLLNDDLQFFETAKDFALVPEKIEMVNWKPMFEEDEETSEIRSRVYAYYLTHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNH1</accession>
    <name>C5DNH1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0G16984p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0G16984g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555769.1">
        <property type="nucleotide sequence ID" value="XM_002555723.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNH1"/>
    <dbReference type="FunCoup" id="C5DNH1">
        <property type="interactions" value="587"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNH1"/>
    <dbReference type="GeneID" id="8294060"/>
    <dbReference type="KEGG" id="lth:KLTH0G16984g"/>
    <dbReference type="eggNOG" id="ENOG502S7CA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_444861_0_0_1"/>
    <dbReference type="InParanoid" id="C5DNH1"/>
    <dbReference type="OMA" id="HHYQDML"/>
    <dbReference type="OrthoDB" id="5600085at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006878">
        <property type="term" value="P:intracellular copper ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.430.10:FF:000001">
        <property type="entry name" value="Copper fist DNA-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.430.10">
        <property type="entry name" value="Copper fist DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051763">
        <property type="entry name" value="Copper_Homeo_Regul"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001083">
        <property type="entry name" value="Cu_fist_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036395">
        <property type="entry name" value="Cu_fist_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28088">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR HAA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28088:SF5">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR HAA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00649">
        <property type="entry name" value="Copper-fist"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00617">
        <property type="entry name" value="COPPERFIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM01090">
        <property type="entry name" value="Copper-fist"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00412">
        <property type="entry name" value="Cu_FIST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57879">
        <property type="entry name" value="Zinc domain conserved in yeast copper-regulated transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01119">
        <property type="entry name" value="COPPER_FIST_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50073">
        <property type="entry name" value="COPPER_FIST_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0186">Copper</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Copper-fist" evidence="9">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="104"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="330"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="452"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="111"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="344"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="460"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="476"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="500"/>
            <end position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR25332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="614" mass="66914" checksum="331D71DEBAACB048" modified="2009-07-28" version="1">MVLVNGIKYACERCIRGHRVTTCNHTDQPLMMIKPKGRPSTTCAHCKELRKNKNANPTGGCTCGRQQKKRLAQKAKEEARAKAKSKDDSCRCIETDKCTCHARRKNRRSVSSRSRTTTTTTSSIASEDSPTMPTCDGPDFLGRIAKSHAMGSLPSFHSSQSLDRDFGLATSPVLSPSLSNYHGNSSNWDANSITSLKSDSRINLSDRSHQSIGLDSLHSTRAFPPQARARVGELPIPMEDYSSDLNSLSANSNFNPSGSSNRVAWSEADPNHGLLDLFADTSENVNTNYMAMKSSLKRNKSYASGLGVMTPQCAGKDPYAQITRSQSYQRDVPKHLVSRPSVDSVISHSSKVSEGANNGRETLQSNHSLPFITARQLQPRMSYGGNNFPPGCLSLDDDSAKSVEVLSLTPSFMDIPDNTNFYSATSNPFLHQQKVEPRKRSVSIHRNHRYDSFTRGASGESHTPVIQQTQSPHSRQGGEVKAEATTRAESQDADSDSAFPLSQVTPSGPSMNISPGADSNSLTQTSHVSSPRDTLTNQFMEPTSSFTSEIDHILGSNNDDVQSLFSDPTISLDATHSMNLEASPIDQAHRMPSLCYSETTGASNLDDLDKLMAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNI2</accession>
    <name>C5DNI2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">tRNA(His) guanylyltransferase</fullName>
            <ecNumber evidence="2 12">2.7.7.79</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 12">tRNA-histidine guanylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">KLTH0G17270g</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Adds a GMP to the 5'-end of tRNA(His) after transcription and RNase P cleavage.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a 5'-end ribonucleotide-tRNA(His) + GTP + ATP + H2O = a 5'-end phospho-guanosine-ribonucleotide-tRNA(His) + AMP + 2 diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54564"/>
            <dbReference type="Rhea" id="RHEA-COMP:14193"/>
            <dbReference type="Rhea" id="RHEA-COMP:14917"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:138282"/>
            <dbReference type="ChEBI" id="CHEBI:141847"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.7.79"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="14">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 12">Belongs to the tRNA(His) guanylyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.79" evidence="2 12"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555780.1">
        <property type="nucleotide sequence ID" value="XM_002555734.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNI2"/>
    <dbReference type="FunCoup" id="C5DNI2">
        <property type="interactions" value="798"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNI2"/>
    <dbReference type="GeneID" id="8294073"/>
    <dbReference type="KEGG" id="lth:KLTH0G17270g"/>
    <dbReference type="eggNOG" id="KOG2721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044271_0_1_1"/>
    <dbReference type="InParanoid" id="C5DNI2"/>
    <dbReference type="OMA" id="WKQHTEI"/>
    <dbReference type="OrthoDB" id="62560at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008193">
        <property type="term" value="F:tRNA guanylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3000:FF:000003">
        <property type="entry name" value="tRNA(His) guanylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025845">
        <property type="entry name" value="Thg1_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024956">
        <property type="entry name" value="tRNAHis_GuaTrfase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007537">
        <property type="entry name" value="tRNAHis_GuaTrfase_Thg1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038469">
        <property type="entry name" value="tRNAHis_GuaTrfase_Thg1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12729">
        <property type="entry name" value="TRNA(HIS) GUANYLYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12729:SF6">
        <property type="entry name" value="TRNA(HIS) GUANYLYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04446">
        <property type="entry name" value="Thg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14413">
        <property type="entry name" value="Thg1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028980">
        <property type="entry name" value="tRNAHis_guanylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12 13" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9 12 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 12 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 12" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 12" id="KW-0819">tRNA processing</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="tRNAHis guanylyltransferase catalytic" evidence="16">
        <location>
            <begin position="6"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="Thg1 C-terminal" evidence="17">
        <location>
            <begin position="142"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="29"/>
            <end position="34"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="76"/>
            <end position="77"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR028980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR028980-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR028980-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF04446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF14413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAR25343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="237" mass="27754" checksum="F3DF4E30697533AA" modified="2009-07-28" version="1">MAKSRFEYVRQFESHETLLPETYIVVRIDGKKFHEFSKHYNFEKPNDERALKLMNASAKNVVLKYKNDVVLAFGESDEYSFILKKDTSLFKRRRDKLSSLFVSLFTANYVALWSKFFPGTALDYKHLPFFDSRCVCYPNLSTIKDYLCWRYVDTHINNLYNTVFWQLIIKCGLTPQESEQKLCGTLSSDKQEILFTDCGINYNDEPNMFKKGSLVTNKGEVCHIDVVKQIDVLFEGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNM6</accession>
    <name>C5DNM6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0G18370p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0G18370g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555824.1">
        <property type="nucleotide sequence ID" value="XM_002555778.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNM6"/>
    <dbReference type="FunCoup" id="C5DNM6">
        <property type="interactions" value="87"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNM6"/>
    <dbReference type="GeneID" id="8294119"/>
    <dbReference type="KEGG" id="lth:KLTH0G18370g"/>
    <dbReference type="eggNOG" id="KOG2595">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039465_3_0_1"/>
    <dbReference type="InParanoid" id="C5DNM6"/>
    <dbReference type="OMA" id="YLRDFMM"/>
    <dbReference type="OrthoDB" id="206700at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.1310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045913">
        <property type="entry name" value="TBC20/Gyp8-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20913:SF7">
        <property type="entry name" value="RE60063P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20913">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 20/GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="3">
        <location>
            <begin position="63"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="177"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR25387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="512" mass="57363" checksum="C1BCE2F4173089DE" modified="2009-07-28" version="1">MASSYTVRVNDHEFLSESVMDEKSVVYFRHSERLSKKTHGLSCALADGDLEALAALGRDTDGFLKHELRGPAWLALLKSRLPFDTASSSKNGGGDPHRDEHQIALDVKRSFGDIKDDNAKAFLRHALERTIVRVLRAFPQLSYYQGYHDVCAVFVLVFAHASAPGVGHNLSASTSSTASAEGMDAPHDSDGSDISESLSSSSISTKLADDSLLLVDSEQLFEAVVIFTLLYLRDFMMNSLTFTIDQLALIPLIVKQKDSYFYRTFQLDKIDPFFALSSILTLFSHDLRPQSDDAPSIVFQVFDLVISANSMMVPLVAYSYLLLAKKDDLLLRYKENIDNFDNSTDLIHGVIQQEMIAGSSVQLWNDILDASRNSSLELPRESKKILNKYSVLLTNSRGPILLEEVLTWLSREICLNESVGSRNARLDNKKSPVPKWRLLAQGRGSILVKFSLFIGVAAILLKLYMQSLEGHQGHRRIELYFDQIKSLKFLGLTQPRRVWLDPLKTLLKIGRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>C5E1X3</accession>
    <name>C5E1X3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Glucose-6-phosphate 1-dehydrogenase</fullName>
            <ecNumber evidence="3 11">1.1.1.49</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0H00374g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>D-glucose 6-phosphate + NADP(+) = 6-phospho-D-glucono-1,5-lactone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15841"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57955"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="EC" id="1.1.1.49"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the glucose-6-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.49" evidence="3 11"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555896.1">
        <property type="nucleotide sequence ID" value="XM_002555850.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E1X3"/>
    <dbReference type="FunCoup" id="C5E1X3">
        <property type="interactions" value="550"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E1X3"/>
    <dbReference type="GeneID" id="8294207"/>
    <dbReference type="KEGG" id="lth:KLTH0H00374g"/>
    <dbReference type="eggNOG" id="KOG0563">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013524_2_3_1"/>
    <dbReference type="InParanoid" id="C5E1X3"/>
    <dbReference type="OMA" id="ERAGYYE"/>
    <dbReference type="OrthoDB" id="60984at2759"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00408"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004345">
        <property type="term" value="F:glucose-6-phosphate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009051">
        <property type="term" value="P:pentose-phosphate shunt, oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000015">
        <property type="entry name" value="Glucose-6-phosphate 1-dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000111">
        <property type="entry name" value="Glucose-6-phosphate 1-dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00966">
        <property type="entry name" value="G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001282">
        <property type="entry name" value="G6P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019796">
        <property type="entry name" value="G6P_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022675">
        <property type="entry name" value="G6P_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022674">
        <property type="entry name" value="G6P_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00871">
        <property type="entry name" value="zwf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23429:SF0">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23429">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-DEHYDROGENASE G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02781">
        <property type="entry name" value="G6PD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00479">
        <property type="entry name" value="G6PD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000110">
        <property type="entry name" value="G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00079">
        <property type="entry name" value="G6PDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00069">
        <property type="entry name" value="G6P_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5 11" id="KW-0313">Glucose metabolism</keyword>
    <keyword evidence="6 11" id="KW-0521">NADP</keyword>
    <keyword evidence="7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucose-6-phosphate dehydrogenase NAD-binding" evidence="12">
        <location>
            <begin position="14"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-6-phosphate dehydrogenase C-terminal" evidence="13">
        <location>
            <begin position="197"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR30034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="511" mass="58077" checksum="46685CDCF3ECA6FA" modified="2009-07-28" version="1">MSKPTEFKENTSIVVFGASGDLSKKKTFPALFGLFREGYLDPSCKIIGFARSNLSDEDLHEKIKPNLKTNNDEKGSEKIEQFLKMVSYISGPYDKDEGFDKLAKELDSYEKKQGVKSPHRLFYLALPPSAFVTVATQIKKKVYAEHGETRVVVEKPFGHDLDSFRELQSDLSPLFREDELFRIDHYLGKEMVKNLVFMRFGNTFLNAAWNKENIQMIQISFKEPFGTEGRGGYFDEIGIIRDVMQNHLLQVLTLLTMERPVTFDSEAVRDEKVKVLKAFAPIDHKDILIGQYGKSEDGSKPGYLDDDTVNPDSKCVTFAALGFKIQNERWDGVPIVMRAGKALNEGKVEIRIQFKAVPSGVFSEVAHNELVIRVQPDEAIYIKCNSKTPGLSTTSQVSELDLTYARRFKDFWIPEAYEALIKDVLSGDHSNFVRDDELDISWKLFTPLLKYLEGPDAPKPEEYAYGSRGPAELVNFLKSHDYVFEDSGSYQWPVITPEVTKKKQNSTDSKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>C5E1Y8</accession>
    <name>C5E1Y8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0H00726p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0H00726g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555911.1">
        <property type="nucleotide sequence ID" value="XM_002555865.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5E1Y8">
        <property type="interactions" value="1423"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E1Y8"/>
    <dbReference type="GeneID" id="8294223"/>
    <dbReference type="KEGG" id="lth:KLTH0H00726g"/>
    <dbReference type="eggNOG" id="KOG1795">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000380_3_0_1"/>
    <dbReference type="InParanoid" id="C5E1Y8"/>
    <dbReference type="OMA" id="ANKWNTS"/>
    <dbReference type="OrthoDB" id="1931567at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097157">
        <property type="term" value="F:pre-mRNA intronic binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030619">
        <property type="term" value="F:U1 snRNA binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030620">
        <property type="term" value="F:U2 snRNA binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:U5 snRNA binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017070">
        <property type="term" value="F:U6 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000393">
        <property type="term" value="P:spliceosomal conformational changes to generate catalytic conformation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000244">
        <property type="term" value="P:spliceosomal tri-snRNP complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08056">
        <property type="entry name" value="MPN_PRP8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13838">
        <property type="entry name" value="RNase_H_like_Prp8_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.40:FF:000001">
        <property type="entry name" value="Pre-mRNA-processing-splicing factor 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.230:FF:000001">
        <property type="entry name" value="Pre-mRNA-processing-splicing factor 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000002">
        <property type="entry name" value="Pre-mRNA-processing-splicing factor 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1570.40:FF:000001">
        <property type="entry name" value="Pre-mRNA-processing-splicing factor 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1570.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.40">
        <property type="entry name" value="Pre-mRNA-processing-splicing factor 8, U5-snRNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012591">
        <property type="entry name" value="PRO8NT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012592">
        <property type="entry name" value="PROCN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012984">
        <property type="entry name" value="PROCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027652">
        <property type="entry name" value="PRP8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021983">
        <property type="entry name" value="PRP8_domainIV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043173">
        <property type="entry name" value="Prp8_domainIV_fingers"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043172">
        <property type="entry name" value="Prp8_domainIV_palm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019581">
        <property type="entry name" value="Prp8_U5-snRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042516">
        <property type="entry name" value="Prp8_U5-snRNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019580">
        <property type="entry name" value="Prp8_U6-snRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019582">
        <property type="entry name" value="RRM_spliceosomal_PrP8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11140">
        <property type="entry name" value="PRE-MRNA SPLICING FACTOR PRP8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11140:SF0">
        <property type="entry name" value="PRE-MRNA-PROCESSING-SPLICING FACTOR 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08082">
        <property type="entry name" value="PRO8NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08083">
        <property type="entry name" value="PROCN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08084">
        <property type="entry name" value="PROCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12134">
        <property type="entry name" value="PRP8_domainIV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10598">
        <property type="entry name" value="RRM_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10597">
        <property type="entry name" value="U5_2-snRNA_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10596">
        <property type="entry name" value="U6-snRNA_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00232">
        <property type="entry name" value="JAB_MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="MPN" evidence="8">
        <location>
            <begin position="2186"/>
            <end position="2312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="33"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR30049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="2414" mass="278350" checksum="CE30205EEBF6A5DE" modified="2009-07-28" version="1">MSGLPPPPPPPGFNASVPPPPPPGFRKRTSVSRSEDDFNVKDLKEQLLPPPPPPPMPSSISALPPPPPHHHHHKGPDTSKPALKKQKLLSHCLESPRLSVEELKERKREWISSQKKRFRQSSSKCARGKTQKRGVIHSHKVDMPPEHLRKIINDHKDMSSKRFIHDKRAFLGALKYMPHAILKLLENMPQPWEQVKEVRVLYHNTGALTFVNEIPRVIEPVFTAQWSAAWIAMRREKRDRRHFKRMRFPPFDDEEPPLSYHEHIQGIEPLDGITLQLDEVDDALIKDWIFDSKPLLDEKSCVNGSSYKTWSIDHRTMANLHRLSSPLLDSGLDSNKNYLFEKKSFFTAKALNNAIPGGPKFEPLFSAEEENDINEFNSLDRVIFRVPIRTGYRIAFPHLYNSRPRAVQLSWYHTPIACLAEETQDENAETFRFDPSFNLISASYGTSRPLSLESEDFLLDANFRPLLSSEKVCLPDTKAAISLYHAPYPFNLRSGRTVRAQDVALVKKWYLQKPETDVPVKVRVSYQKLLKNYVLNELKSSKLGKQSKKKLLRSLRNTKFFQQTSIDWVEAGLQLCTQGFNMLNLLIHRKGLTYLHLDYNFNLKPTKTLTTKERKKSRFGHAFHLMREILKAIKLLVDAHVQYRLGNVDAFQLADGVYYILNHLGQLTGIYRYKYKVMHQIRACKDLKHVVYNRFNRVIGKGPGCGFWQPAWRVWIFFMRGIIPLLERWLGNLLARQFEGRSSEVIKTATKQRLDAYYDLELRAAVMNDILDMMPEGIRQNKSKTILQHLSEAWRCWKANIAWNVPGMPEPIKNIIERYIKAKADGWVSAAHYNRDRIKRGAHVEKTVVRKNLGRLTRLWIKHEQERQQLIEKNGPEITPLVATTIFKTMVDWLEQNNFSPVPFPPLTYKNDTKILVLALENLKNAFASKVRLNASEREELALIEEAYDNPHDTLNRIKKYLLTQRVFKPVDLSMKDQLQTISPVYSVDPLEKITDAYLDQYLWYEADKRQLFPNWIKPSDTEIPPLLVYKWAQGINNLSEVWDVNNGQSTVLLQTKLEGLAEKIDLTLLNRLLRLVMDPNLADYITAKNNVVLNFKDMSHVNKYGLIRGLQFSSFVYQFYGLMLDLLILGPQRASELAGPSQAPNNFLQFKSLDVEKRNPIRLYCRYFDVVHILFHFEEQDSEALVLDFLSENPDPNFENAVGYNNKKCWPKDARMRLMRPDVNLGRAVFWEISNRVPISLVNITWENSFASVYSKSNPNLLFTMCGFEARILPNFRTEEMISFDEGVWDLVDERTKQRTAKTFLRVSDEHVEKFNDTIRGILMASGSTTFTKITARWNTALISLFTYFREAVVSTESLLDVLVKCETKIQNRVKLGLNSKMPTRFPPAVFYTPKELGGLGMLSASHILIPASDLSWSKQTDTGITHFRAGMTHEDEKLIPTIFRYITTWENEFLDSQRVWADYVAKRQEALKQNRRLAFEELEGSWDRGIPRISTLFQKDRHTLAYDKGHRARSQFKDFSLERNNPFWWTDTHHDGKLWNLNAYRTDVIQALGGIETILEHTLFKGTGFNSWEGLFWEKASGFEDSMQFKKLTHAQRTGLSQIPNRRFTLWWSPTINRANVYVGFLVQLDLTGIFLHGKIPTLKISLIQIFRAHLWQKIHESIVFDVCQVLDGEMDMLQIESVEKEAIHPRKSYKMNSSAADVTLSSNHTWNVSSPSLLHNTNDNFNVSNSSKVWIDVQLRYGDYDSHDISRYVRAKFLDYTTDNVSMYPSPTGVMIGIDLAYNMYDAYGNWFDGLKTLLQNGMKTIMKANPSLYVLRERIRKGLQIYQSQTQEPFLNSSNYAELFSDETKLFIDDTNVYRVAVHTTYEGNIATKPINGCIFTLNPKTGELFLKIIHTSVWAGQKRLSQLAKWKTAEETSALIRSLPKEEQPKQVIVTRKAMLDPLEVHMLDFPNISIRPTELKLPFSASMAIEKLSDVVTKATEPQMVLFNIYDDWLERVSSYTAFSRLILLLRALRTNEERAKMILKADPTIPIKEHHLWPSFSDDQWIEIESQMRDLILVEYGKKYNVNIASLTQTEIKDLILGQNIKAPSVKRQQMAELEMSKTTQNSNGLDSSSGSAMKIKSTNAQGEEIMVVTSTNFETQTFSTKNEWRKRAIANSLLYMRLKTIYLASDDFVEEKDVFILPKNLLKRFVEISDTKAQVAGYIFGKKAAGHEKVKEIRTIVIVPQLGNTHTVQLSMLPEYSPYLQDLELLGWTHTQSQELKYMSAPEVTTHSNIFKGEKDLVSLSVCLNPGSISLAAYTVSEEGYSWGSSNKNLFDAEPQGFDPEFSEHAQLMLSDRVTGNFLVPSNDVWNYAFMSAVFSPSAKYELKLDIPIEFYNELHRSIHFLQFSDMVENEELEPEKEDVFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-04-02" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2E4</accession>
    <name>C5E2E4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0H04246p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0H04246g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556067.1">
        <property type="nucleotide sequence ID" value="XM_002556021.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2E4"/>
    <dbReference type="STRING" id="559295.C5E2E4"/>
    <dbReference type="GeneID" id="8294380"/>
    <dbReference type="KEGG" id="lth:KLTH0H04246g"/>
    <dbReference type="eggNOG" id="KOG4818">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_528999_0_0_1"/>
    <dbReference type="InParanoid" id="C5E2E4"/>
    <dbReference type="OMA" id="PPMCEIN"/>
    <dbReference type="OrthoDB" id="4068596at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010106">
        <property type="term" value="P:cellular response to iron ion starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014842">
        <property type="entry name" value="AFT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08731">
        <property type="entry name" value="AFT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="274"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="491"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="33"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="285"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="298"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="316"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="349"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR30205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="520" mass="58267" checksum="0E503C679CE771C6" modified="2009-07-28" version="1">MDQSALDAFHGELIQTSPSLDFLLAEDSPLSTVDTVGTGTTSNTESSGPDFTNGTPSWNDTASLNNMLSENALESINRQRSQNKLIHLDPIPDFQDRNEIKPWLQKIFYPQGIEIVIERSDNIKVVFKCKASKRGRASRKQAGCGSLPEEKENPESPLAVKRVQQANKKKRSVSPYNTCPFRVRATYSLKRKKWNIVVMNNGHSHPLKFNADSDDYKKFKNYLRENEDWDAVKKFDELECRTRFNLPTQPQPIPCDCGLTQEIESFNIVLPTSDSLANSTDKTVSKPRHKSTRKGNKQMATALSRNSTPLGFLDKSPSQSARSGQEPQRIKQESTGAPQEPHSGMTGLSPMQNFDTSMSGWDPNTLTSQSEEIDFTDLFLRPLPRTKQDPIFEKPQTLNSPGRDQPWNASKNFMDDLQFQLAPVCEPSHTSNAHCILPSSTEAHMNCDAMDAGSQHEFPHHRVQQQDPSMLHSSPANGMLSSDLGNMHLTGQQMQVPHPDPGLPWEPQHSNDPHGYSNDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2M8</accession>
    <name>C5E2M8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein uS5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">40S ribosomal protein S2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0H06248g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the universal ribosomal protein uS5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556151.1">
        <property type="nucleotide sequence ID" value="XM_002556105.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2M8"/>
    <dbReference type="FunCoup" id="C5E2M8">
        <property type="interactions" value="941"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2M8"/>
    <dbReference type="GeneID" id="8294466"/>
    <dbReference type="KEGG" id="lth:KLTH0H06248g"/>
    <dbReference type="eggNOG" id="KOG0877">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065898_0_2_1"/>
    <dbReference type="InParanoid" id="C5E2M8"/>
    <dbReference type="OMA" id="PYEEWSD"/>
    <dbReference type="OrthoDB" id="10253125at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000002">
        <property type="entry name" value="40S ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000004">
        <property type="entry name" value="40S ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000851">
        <property type="entry name" value="Ribosomal_uS5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005324">
        <property type="entry name" value="Ribosomal_uS5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005711">
        <property type="entry name" value="Ribosomal_uS5_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013810">
        <property type="entry name" value="Ribosomal_uS5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018192">
        <property type="entry name" value="Ribosomal_uS5_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01020">
        <property type="entry name" value="uS5_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13718:SF4">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13718">
        <property type="entry name" value="RIBOSOMAL S SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00333">
        <property type="entry name" value="Ribosomal_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03719">
        <property type="entry name" value="Ribosomal_S5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00585">
        <property type="entry name" value="RIBOSOMAL_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50881">
        <property type="entry name" value="S5_DSRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="S5 DRBM" evidence="9">
        <location>
            <begin position="80"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="22"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR30289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="258" mass="27785" checksum="A802C7A29390E65F" modified="2009-07-28" version="1">MSAPAPAPAQGDQRRGGFGGRNKGRQQRRGRRDQEEKGWTPVTKLGRLVKAGKITSVEEIFLHSLPVKEFQIVDTLLPTLKDEVMNIKPVQKQTRAGQRTRFKAVVVVGDSNGHVGLGIKTAKEVAGAIRAGIIIAKLSVIPIRRGYWGNNLGQPHSLATKTSGKCGSVTVRLIPAPRGKGIVASPAVKKLLELAGVEDVYTSSNGSTRTLENTLKATFVAIGNTYGFLTPNLWAAQPLPVSPLDIYSDEASAEKKRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3C3</accession>
    <name>C5E3C3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ribosome biogenesis protein RLP7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0H12166g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in the biogenesis of the 60S ribosomal subunit. May act as a specificity factor that binds precursor rRNAs and tethers the enzymes that carry out the early 5' to 3' exonucleolytic reactions that generate the mature rRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uL30 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556396.1">
        <property type="nucleotide sequence ID" value="XM_002556350.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3C3"/>
    <dbReference type="FunCoup" id="C5E3C3">
        <property type="interactions" value="412"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3C3"/>
    <dbReference type="GeneID" id="8294733"/>
    <dbReference type="KEGG" id="lth:KLTH0H12166g"/>
    <dbReference type="eggNOG" id="KOG3184">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055156_1_0_1"/>
    <dbReference type="InParanoid" id="C5E3C3"/>
    <dbReference type="OMA" id="VNGWGPQ"/>
    <dbReference type="OrthoDB" id="28644at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01657">
        <property type="entry name" value="Ribosomal_L7_archeal_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.20">
        <property type="entry name" value="Ribosomal protein L30, ferredoxin-like fold domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036919">
        <property type="entry name" value="Ribo_uL30_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039699">
        <property type="entry name" value="Ribosomal_uL30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018038">
        <property type="entry name" value="Ribosomal_uL30_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035808">
        <property type="entry name" value="Ribosomal_uL30_euk_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016082">
        <property type="entry name" value="Ribosomal_uL30_ferredoxin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524:SF26">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN RLP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00327">
        <property type="entry name" value="Ribosomal_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55129">
        <property type="entry name" value="Ribosomal protein L30p/L7e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00634">
        <property type="entry name" value="RIBOSOMAL_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL30-like ferredoxin-like fold" evidence="9">
        <location>
            <begin position="130"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="34"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR30534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="310" mass="35124" checksum="8A74D189F00D1BCA" modified="2009-07-28" version="1">MVTGLNSNPEILLRKRRNADRTRLEKQELAQRRQQLKQKQARARKNKFERAETIVATSLATQREKERIKRVSKLEAKRAKGDNEHLASTRDFILKVHDSDAESQAQGDHDEEDDSLHTEKCAYDGKPTLLFVVRIRGPTAVKIPQKAHKVLSLLRLSELNTGVFVRLTAQVYPLLKLIAPYIVVGEPSLASVRSLIHKRSRVLQSTPEGEEPKETILNDNNLVEEKLGSEGIICVEDIIHEIATLGESFSKCNFFLLPFRLNREVSGFSALSKLQKIRQREWQKRALKVSNASTAPIVQVDIDSLIAKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBD9</accession>
    <name>C5DBD9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0A01804p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0A01804g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SCO1/2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551538.1">
        <property type="nucleotide sequence ID" value="XM_002551492.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBD9"/>
    <dbReference type="FunCoup" id="C5DBD9">
        <property type="interactions" value="577"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBD9"/>
    <dbReference type="GeneID" id="8290333"/>
    <dbReference type="KEGG" id="lth:KLTH0A01804g"/>
    <dbReference type="eggNOG" id="KOG2792">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050131_0_1_1"/>
    <dbReference type="InParanoid" id="C5DBD9"/>
    <dbReference type="OMA" id="MLYFRVE"/>
    <dbReference type="OrthoDB" id="270009at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02968">
        <property type="entry name" value="SCO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000013">
        <property type="entry name" value="Blast:Protein SCO1 homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003782">
        <property type="entry name" value="SCO1/SenC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12151:SF5">
        <property type="entry name" value="AT19154P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12151">
        <property type="entry name" value="ELECTRON TRANSPORT PROTIN SCO1/SENC FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02630">
        <property type="entry name" value="SCO1-SenC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0186">Copper</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="6">
        <location>
            <begin position="147"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="32"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="67"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="4">
        <location>
            <begin position="185"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR603782-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR603782-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR21096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="331" mass="37174" checksum="F51F509B0208D6D0" modified="2009-07-28" version="1">MLSRTGVFTVLKRSTRLSGYALRPLSTRSCLRVEDQGTSRSRVTPRGGAPSGASSSTDPSDQSAFSGNTPPKSTTFEDADPKRKPLSRIAIGGTETKSQKATSGSIEFATWKAAALVLTLGGTLYYFFSKEKRRLEIEKEAEANRGYGKPLVGGPFKLVDFNGNEFTEKNLLGKFSIIYFGFSHCPDICPDELDKLSEWLDGLKKKGIELQPIFITCDPARDPPHVLKEYLSEFHPDLIGLTGEYNDIKNACKQYRVYFSTPPSLKPGQDYLVDHSIFFYLMDPEGQFIDALGRQYDAETGVAKIEEHVKAYVPREQREKRKEKWYSFLFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBG9</accession>
    <name>C5DBG9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Coatomer subunit zeta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0A02508g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. The zeta subunit may be involved in regulating the coat assembly and, hence, the rate of biosynthetic protein transport due to its association-dissociation properties with the coatomer complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 12">Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 12">Golgi apparatus membrane</location>
            <topology evidence="1 12">Peripheral membrane protein</topology>
            <orientation evidence="1 12">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Cytoplasmic vesicle</location>
            <location evidence="12">COPI-coated vesicle membrane</location>
            <topology evidence="12">Peripheral membrane protein</topology>
            <orientation evidence="12">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the adaptor complexes small subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551568.1">
        <property type="nucleotide sequence ID" value="XM_002551522.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBG9"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="HOGENOM" id="CLU_086803_0_0_1"/>
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        <property type="component" value="Chromosome A"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0006886">
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    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
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        <property type="entry name" value="Coatomer subunit zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
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    <dbReference type="InterPro" id="IPR039652">
        <property type="entry name" value="Coatomer_zeta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11043:SF0">
        <property type="entry name" value="COATOMER SUBUNIT ZETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11043">
        <property type="entry name" value="ZETA-COAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 12" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6 12" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8 12" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AP complex mu/sigma subunit" evidence="13">
        <location>
            <begin position="9"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU366053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR21126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="196" mass="21996" checksum="06F4FF4D20D71E92" modified="2009-07-28" version="1">MAELSLYSVEAVLLLDNEGNRIYTKYYHSPHEKSDSHITKRAIDGMSSSSKQQTEYESRLFKKTHKQNSEILIFEDCLVLYKEYVDVSLYLVGSIDENELVLQQAFSAIKDSLELILATGIDKKNIIEHFDMVALAIDESIDDGIILETDPATIASRVTKPPSKDAPINIELSEKGLLSAWGFAKSKLAERLQQGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-04-02" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBI0</accession>
    <name>C5DBI0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0A02794p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0A02794g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551579.1">
        <property type="nucleotide sequence ID" value="XM_002551533.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBI0"/>
    <dbReference type="STRING" id="559295.C5DBI0"/>
    <dbReference type="GeneID" id="8290378"/>
    <dbReference type="KEGG" id="lth:KLTH0A02794g"/>
    <dbReference type="eggNOG" id="ENOG502S4J9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091402_0_0_1"/>
    <dbReference type="InParanoid" id="C5DBI0"/>
    <dbReference type="OMA" id="RWNDQYK"/>
    <dbReference type="OrthoDB" id="2444812at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WW" evidence="2">
        <location>
            <begin position="9"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="80"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR21137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="216" mass="23508" checksum="FD19050EE202106F" modified="2009-07-28" version="1">MGHSKSNPPKVPNGWKAVFDEEYRTYFFVDLSNGKSQWEEPKGTTWNAESPPPYQEQRPAGAPSAVKGDRKTPATEQRSGYPQQAYPQQPYSQQAYPQQGYGQQAYPQQGYPQQGYPQQGYYPQAYPQQPYYPQAYPQQQYPQQQQGRKKFGGAMTGGLLGVGGGLLAGSLLGSALSPHETIVENNYYDDNGGGDYGGGDFGGGDFGGGDFGGGDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBM1</accession>
    <name>C5DBM1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0A03718p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0A03718g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551620.1">
        <property type="nucleotide sequence ID" value="XM_002551574.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBM1"/>
    <dbReference type="FunCoup" id="C5DBM1">
        <property type="interactions" value="108"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBM1"/>
    <dbReference type="GeneID" id="8290420"/>
    <dbReference type="KEGG" id="lth:KLTH0A03718g"/>
    <dbReference type="eggNOG" id="KOG3662">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021690_1_0_1"/>
    <dbReference type="InParanoid" id="C5DBM1"/>
    <dbReference type="OMA" id="GLKEQNH"/>
    <dbReference type="OrthoDB" id="9984693at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08164">
        <property type="entry name" value="MPP_Ted1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033308">
        <property type="entry name" value="PGAP5/Cdc1/Ted1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033307">
        <property type="entry name" value="Ted1_MPase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13315">
        <property type="entry name" value="METALLO PHOSPHOESTERASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13315:SF1">
        <property type="entry name" value="PROTEIN TED1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="453"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR21178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="474" mass="55613" checksum="24B888869E54B5F1" modified="2009-07-28" version="1">MIRPLLKKFTGFTLVLAIVSNIYIFTYPSLFPERCSWRCASQPSVVGATDSMHVGEKVLYYSKRYVNDLMRQWKKSHEETQSSPADVHLMAFGDPQIKGIWRNTPYVTRLDIFGNDFYLGHIFSTMRNRLNPTHVAVMGDLFSSQWIGDSEFFNRTMRYTKRIFKRDTTSLENIRNDSHDENGLYKNYWVQFADEFKRRLTEEPKTLGFGYENVHTWDPEHEDYLFINLTGNHDIGYSGDATFQHMSRFHQLFGKDNYWIEYDRDTDHPWRIVVLDSMLLEGPPLQQEFVNATWEFLLQLFEQRFEGSTVLLTHVPFYKKEGLCTDGELFRYYPDDYPREPYKQNLLRSQNHLSEDVTKRVLNLIFDNGKPGIILTGHDHEGCETVYNKDSKTGDWNAAKTMQSPDFHVREVTVRSMMGEFNGNTGLVTGHFNHETKTWEWSFSLCPFSIQHVWWFTKVVTILSGFLLSALLVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBM7</accession>
    <name>C5DBM7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein-serine/threonine kinase</fullName>
            <ecNumber evidence="10">2.7.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0A03916g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the PDK/BCKDK protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.-" evidence="10"/>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551626.1">
        <property type="nucleotide sequence ID" value="XM_002551580.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBM7"/>
    <dbReference type="FunCoup" id="C5DBM7">
        <property type="interactions" value="365"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBM7"/>
    <dbReference type="GeneID" id="8290429"/>
    <dbReference type="KEGG" id="lth:KLTH0A03916g"/>
    <dbReference type="eggNOG" id="KOG0787">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023861_4_1_1"/>
    <dbReference type="InParanoid" id="C5DBM7"/>
    <dbReference type="OMA" id="WSYPPSA"/>
    <dbReference type="OrthoDB" id="3264224at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004740">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010906">
        <property type="term" value="P:regulation of glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.20">
        <property type="entry name" value="Alpha-ketoacid/pyruvate dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036784">
        <property type="entry name" value="AK/P_DHK_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018955">
        <property type="entry name" value="BCDHK/PDK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039028">
        <property type="entry name" value="BCKD/PDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947:SF20">
        <property type="entry name" value="[3-METHYL-2-OXOBUTANOATE DEHYDROGENASE [LIPOAMIDE]] KINASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10436">
        <property type="entry name" value="BCDHK_Adom3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69012">
        <property type="entry name" value="alpha-ketoacid dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Histidine kinase" evidence="11">
        <location>
            <begin position="277"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU366032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR21184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="413" mass="47211" checksum="767B57A0F94EE9C1" modified="2009-07-28" version="1">MFARHSQLAGRGLKQLAITVKLPRLIFHSCATRQSYSTKTSMREKLKEVGFEELYRIRSNTQLLIQDFAKKPIPPLSYEFLTQYKVPLTENQKYTLTIQTVNLLLMYTCRRLVAIQRLPYIAVLNPNIEQTNRLYLRTLESLLSLNYPYGLHDQGVMTKKLTEFLDDHQDTLVTLSRGFQEIMDFFPKEAVFDFLNLHLRDRIAMKLLATHYLALVSQKSNEEPVIGVLHKNLKISELVRRVEEFVGDLCFVKYDHQLPIEILEGEDVTFPCIPTDLEYVLTEILKNSSRAHIENSVPENNTAEKPIEVTIVRNDNDLKIRIRDFGGGIPPDVEDRMFDYSYSTVNSDAKDSGMSAYVIPGEDVCNVSGMGFGLPMCKAYMEMFGGSVDIQSLWGWGTDAYICLKGPSPNLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBP1</accession>
    <name>C5DBP1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0A04224p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0A04224g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RutC family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551640.1">
        <property type="nucleotide sequence ID" value="XM_002551594.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBP1"/>
    <dbReference type="FunCoup" id="C5DBP1">
        <property type="interactions" value="549"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBP1"/>
    <dbReference type="GeneID" id="8290443"/>
    <dbReference type="KEGG" id="lth:KLTH0A04224g"/>
    <dbReference type="eggNOG" id="KOG2317">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100715_7_2_1"/>
    <dbReference type="InParanoid" id="C5DBP1"/>
    <dbReference type="OMA" id="GSYFKEP"/>
    <dbReference type="OrthoDB" id="309640at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019239">
        <property type="term" value="F:deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00448">
        <property type="entry name" value="YjgF_YER057c_UK114_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.40:FF:000001">
        <property type="entry name" value="L-PSP family endoribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.40">
        <property type="entry name" value="RutC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006056">
        <property type="entry name" value="RidA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019897">
        <property type="entry name" value="RidA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035959">
        <property type="entry name" value="RutC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006175">
        <property type="entry name" value="YjgF/YER057c/UK114"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00004">
        <property type="entry name" value="Rid family detoxifying hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11803">
        <property type="entry name" value="2-IMINOBUTANOATE/2-IMINOPROPANOATE DEAMINASE RIDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11803:SF58">
        <property type="entry name" value="PROTEIN HMF1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01042">
        <property type="entry name" value="Ribonuc_L-PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55298">
        <property type="entry name" value="YjgF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01094">
        <property type="entry name" value="UPF0076"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="141" mass="15800" checksum="295A0C1DD9EF8E6F" modified="2009-07-28" version="1">MFLRNAARQTFRTMSTLTPVRTQLSPPPAASYSQAMKVNNMVFVSGQIPYTAENKRVEGTMTDKAEQVIQNVKNILEEANSALNKIVKVNIFLADINDFAEFNGVYGKYFNEHKPARSCVAVKDLPLNVDLEMEVVAVEKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBQ0</accession>
    <name>C5DBQ0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0A04422p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0A04422g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551649.1">
        <property type="nucleotide sequence ID" value="XM_002551603.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBQ0"/>
    <dbReference type="FunCoup" id="C5DBQ0">
        <property type="interactions" value="78"/>
    </dbReference>
    <dbReference type="GeneID" id="8290452"/>
    <dbReference type="KEGG" id="lth:KLTH0A04422g"/>
    <dbReference type="eggNOG" id="ENOG502QQKM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042690_0_0_1"/>
    <dbReference type="InParanoid" id="C5DBQ0"/>
    <dbReference type="OMA" id="NTYQWDY"/>
    <dbReference type="OrthoDB" id="4068713at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="294"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="308"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="318"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="398" mass="43609" checksum="4F908F5A559AE1FB" modified="2009-07-28" version="1">MEQPETRVAYIRIDNLPPGKTWRQVKYLVGGMIHHTSILQVKMLPPVQSIVPPFIPFQSSIVSLKRSDAQLSRLLMELNGYTWEYYPLVAYAVPPLHVPSNAGYGEGLGLGFGLGSGSAGGSAGTGGSRSGSDVPGSPRAPGSAPVPPMMMPFQPMPAMYFPPLAQEPYARHVQSHGKKMRQVFSEESFRKQMSARGMWQLLLEGFPPCLHWDVNGADGAGLQQSPLLGPPPAVHVKTAHPEHFGKLKWTVLKDFIKLKCHKLLEMDNMGATTNTREFYVGVYEDCEVRTDVEVPEQDAGQEPNGVEASDESPEESTSETATTASAKGSAENELEKATQSISRLSLGPSHRVVSATIYKAIVGFHSRELCDMCRESLQGQEYSLGYKLKVTELPLLEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBR5</accession>
    <name>C5DBR5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0A04774p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0A04774g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551664.1">
        <property type="nucleotide sequence ID" value="XM_002551618.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBR5"/>
    <dbReference type="FunCoup" id="C5DBR5">
        <property type="interactions" value="68"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBR5"/>
    <dbReference type="GeneID" id="8290468"/>
    <dbReference type="KEGG" id="lth:KLTH0A04774g"/>
    <dbReference type="eggNOG" id="KOG4580">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_141247_1_0_1"/>
    <dbReference type="InParanoid" id="C5DBR5"/>
    <dbReference type="OMA" id="MAIMIYA"/>
    <dbReference type="OrthoDB" id="2243669at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033254">
        <property type="term" value="C:vacuolar transporter chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003807">
        <property type="entry name" value="DUF202"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051572">
        <property type="entry name" value="VTC_Complex_Subunit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46140">
        <property type="entry name" value="VACUOLAR TRANSPORTER CHAPERONE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46140:SF1">
        <property type="entry name" value="VACUOLAR TRANSPORTER CHAPERONE COMPLEX SUBUNIT 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02656">
        <property type="entry name" value="DUF202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="98"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="DUF202" evidence="6">
        <location>
            <begin position="24"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR21222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="123" mass="13684" checksum="321570C03847590C" modified="2009-07-28" version="1">MSSAPLLQKAPGKKIALPTRVEPKVFFANERTFLSWLNFTVMLGGLGIGLLNFGDKVGRISAGLFTLVAMATMVYALVTYHWRASAIRRRGSGPYDDRLGPTLLCFFLLIAVVVNFLLRMKYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBX8</accession>
    <name>C5DBX8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">KLTH0A06182p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0A06182g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class I-like SAM-binding methyltransferase superfamily. BUD23/WBSCR22 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551727.1">
        <property type="nucleotide sequence ID" value="XM_002551681.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBX8"/>
    <dbReference type="FunCoup" id="C5DBX8">
        <property type="interactions" value="1038"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBX8"/>
    <dbReference type="GeneID" id="8290532"/>
    <dbReference type="KEGG" id="lth:KLTH0A06182g"/>
    <dbReference type="eggNOG" id="KOG1541">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055194_0_2_1"/>
    <dbReference type="InParanoid" id="C5DBX8"/>
    <dbReference type="OMA" id="WIQEKKE"/>
    <dbReference type="OrthoDB" id="2877at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016435">
        <property type="term" value="F:rRNA (guanine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070476">
        <property type="term" value="P:rRNA (guanine-N7)-methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000017">
        <property type="entry name" value="probable 18S rRNA (Guanine-N(7))-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039769">
        <property type="entry name" value="Bud23-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022238">
        <property type="entry name" value="Bud23_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12734:SF0">
        <property type="entry name" value="18S RRNA (GUANINE-N(7))-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12734">
        <property type="entry name" value="METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12589">
        <property type="entry name" value="WBS_methylT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="10">
        <location>
            <begin position="52"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="18S rRNA (guanine(1575)-N(7))-methyltransferase Bud23 C-terminal" evidence="11">
        <location>
            <begin position="203"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="247"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF12589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR21285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="268" mass="30084" checksum="70307D370A939BA2" modified="2009-07-28" version="1">MSRPEDLAPPEVFYNDSESQKYTSSTRVQHIQAKMSLRALELLNLSHMSFVLDIGCGSGLSGEILTEEGHVWCGVDISPSMLATGLTRELEGDLMLHDMGQGLPFRAGTFDAAISISAIQWLCNADTSYNDPKQRLMRFFNTLFACLKKGGKFVAQFYPKNDDQTEQILGAAKVAGFSGGLVVDDPESKKNKKYYLVLSAGASQEEEQVNLQGVTMNAEELAKTKRDRRKEVESNKVYINRKKELMRKRGRKVAKDSKFTGRKRRPRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>C5DC31</accession>
    <name>C5DC31_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0A07392p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0A07392g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0587 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551780.1">
        <property type="nucleotide sequence ID" value="XM_002551734.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DC31"/>
    <dbReference type="FunCoup" id="C5DC31">
        <property type="interactions" value="594"/>
    </dbReference>
    <dbReference type="GeneID" id="8290587"/>
    <dbReference type="KEGG" id="lth:KLTH0A07392g"/>
    <dbReference type="eggNOG" id="KOG1296">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114688_0_0_1"/>
    <dbReference type="InParanoid" id="C5DC31"/>
    <dbReference type="OMA" id="TAHFVWR"/>
    <dbReference type="OrthoDB" id="10248838at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008584">
        <property type="entry name" value="CXXC_Zn-binding_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12857">
        <property type="entry name" value="CXXC MOTIF CONTAINING ZINC BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12857:SF0">
        <property type="entry name" value="CXXC MOTIF CONTAINING ZINC BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05907">
        <property type="entry name" value="CXXC_Zn-b_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141678">
        <property type="entry name" value="MAL13P1.257-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR21338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="185" mass="20995" checksum="CD74326E495A6106" modified="2009-07-28" version="1">MLYVVLSASVSENIGRLKVKDLDTDPADYAFELVCTNCREKHDSKVTINRLEKHTMSGSRGEASFVMKCKFCGKDCSINLERTQEELYNLEEESNRELVEKTRVYRKKIGIKNVDSSKAILLALDCRGCEALNLDFSNLTFEACLVSGKVMEATFEEENEWYDYDDDAGEEVSITGLQFDIIKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCH9</accession>
    <name>C5DCH9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0B03212p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0B03212g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CSC1 (TC 1.A.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551928.1">
        <property type="nucleotide sequence ID" value="XM_002551882.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCH9"/>
    <dbReference type="FunCoup" id="C5DCH9">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="GeneID" id="8290761"/>
    <dbReference type="KEGG" id="lth:KLTH0B03212g"/>
    <dbReference type="eggNOG" id="KOG1134">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002458_2_0_1"/>
    <dbReference type="InParanoid" id="C5DCH9"/>
    <dbReference type="OMA" id="QKWFFAF"/>
    <dbReference type="OrthoDB" id="1076608at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005227">
        <property type="term" value="F:calcium-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045122">
        <property type="entry name" value="Csc1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003864">
        <property type="entry name" value="CSC1/OSCA1-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032880">
        <property type="entry name" value="CSC1/OSCA1-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018">
        <property type="entry name" value="PROBABLE MEMBRANE PROTEIN DUF221-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018:SF20">
        <property type="entry name" value="SPORULATION-SPECIFIC PROTEIN 75"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02714">
        <property type="entry name" value="RSN1_7TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13967">
        <property type="entry name" value="RSN1_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="93"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="176"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="255"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="597"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="642"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="689"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="737"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="812"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="852"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="877"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like N-terminal transmembrane" evidence="9">
        <location>
            <begin position="93"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like 7TM region" evidence="8">
        <location>
            <begin position="597"/>
            <end position="871"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR21490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="978" mass="112389" checksum="5F89011840A02B6F" modified="2009-07-28" version="1">MPKCCLYSLLPVSAKMASMLGSNQNTPKPEFAKCIQPIIETDYMTTAISMDFNTASFRSENRHYSITESILTTFLNTTRAGSAQKHTGMSFDALISSLVVSIVYCGLQTLLFTMLRGKLKTLYQTRNFQLSEANEMRSLQENGPLGWVKSAWKTPLNEYKRANGLDSFFFLRFMKAFGLFFLSLSVINIPVLIPIHYCSGAKVLRQRCKIDSLQAFNETEARLTSHFLEIEDSLKAQGLDKLSISNVSLQHADRLIFHFILAAFVVLWFHALLIKELKFFIAERNKALSNNEVPGSFYQSVLFLDNIPRDMFLNRAQLPKLFRFADSNDIQLTFLPTNYQRLKKYYQKDKKIIDKLEHLQLKLIVLQSNQCGTQKALPPNERAVSGFATDVSDKYAVRTNKLGRKAESAQQCYGPERSKKIYNNGTARKIRKEKVSRYGCARWASKLRLSSHNLNITIKHISKQKSHIKINTRWLRALGIPSSFNIIKFRLNKDQARESLMKALEKLATELIQNREKWKELSEKPTPFVADGDQKQEEHPKKLFITFKSSRTAHLFAQVLISDKFKELNNVIIGPNPIDMIWSNVIQSSRWLKVVRIIFADLIAVLVILGWVLPVAFVGLISQVPNMKILVPFFTWLDSDNAFFSSVLSSLVPILFLIFLIDIVPFIFRWLSILKVNRTGAEIELDVQRWLFVFFFVHIFLVVTVSSGISVIFEKLINNPTSLPDLLAANLPKSSNFFYSFIFIRGLAYFGGNLLQVKELLFELFYYRWCIRTPRKVRKRMEVVSENMWGSVYPLFSTIASIGIVYSVIAPLVLIFATVTFLIVQFSFKCRLNHQQHSRGKSETFGKLYPQALMQSYAGIYCLEICMIGLFALSNRYVLVICMALVFIFTILAHFKISKFYDKYINFLPLKEDEESLLTEHPRKDLFPFFVKEKCLWIPQDSSGASNDQCHLMNEKFDIFCRNEGCFFDGATGSLNFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCL9</accession>
    <name>C5DCL9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosome biogenesis protein ERB1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Eukaryotic ribosome biogenesis protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ERB1</name>
        <name evidence="10" type="ordered locus">KLTH0B04136g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the NOP7 complex, which is required for maturation of the 25S and 5.8S ribosomal RNAs and formation of the 60S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the NOP7 complex, composed of ERB1, NOP7 and YTM1. Within the NOP7 complex ERB1 appears to interact directly with NOP7 and YTM1. The NOP7 complex also associates with the 66S pre-ribosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
            <location evidence="6">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
            <location evidence="6">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the WD repeat BOP1/ERB1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551968.1">
        <property type="nucleotide sequence ID" value="XM_002551922.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCL9"/>
    <dbReference type="FunCoup" id="C5DCL9">
        <property type="interactions" value="1151"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCL9"/>
    <dbReference type="GeneID" id="8290803"/>
    <dbReference type="KEGG" id="lth:KLTH0B04136g"/>
    <dbReference type="eggNOG" id="KOG0650">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011390_0_1_1"/>
    <dbReference type="InParanoid" id="C5DCL9"/>
    <dbReference type="OMA" id="MRPAKGE"/>
    <dbReference type="OrthoDB" id="5571054at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070545">
        <property type="term" value="C:PeBoW complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043021">
        <property type="term" value="F:ribonucleoprotein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000061">
        <property type="entry name" value="Ribosome biogenesis protein BOP1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03027">
        <property type="entry name" value="BOP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028598">
        <property type="entry name" value="BOP1/Erb1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012953">
        <property type="entry name" value="BOP1_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17605:SF0">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN BOP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17605">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN BOP1 BLOCK OF PROLIFERATION 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08145">
        <property type="entry name" value="BOP1NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01035">
        <property type="entry name" value="BOP1NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 6" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="2 6" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="3 7" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="BOP1 N-terminal" evidence="9">
        <location>
            <begin position="180"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="447"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="790"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="28"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="70"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR21530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="816" mass="92586" checksum="673DAED7D0247DF2" modified="2009-07-28" version="1">MAKNQQAKRLSTTRSNSKGEAEKKRELEEESSDDELNVQGLIDDEADESDEDYSEVDSEADETGQFQSAEEGEDGASEEEAGSDSDEEFNKLLAEEEPEDGSEANESLEFSDASNSNSMVEKLSSIKLNMIPDQDEEVIRTKFSDGRPRTIKPEINPVYDSDDSDVEVQNTIGNIPLSAYDEMPHIGYSINGKRIMRPAKGSALDQLLETIELPEGWTGLLDKDTGASLNLSQEELELISKIQKNEKTDDSVDPYQPYVDWFTRHTETMPLTAVPEPKRRFVPSKNEAKRIMKIVRAIREGRIIPPNKLKEMREEEESSHHYDLWGASDESDDHVMNLRAPKLPPPTNEESYNPPEEYLLSKEEKEEWENQEPSERERNFVPQKFNALRKVPGYVESVRERFERSLDLYLAPRVRKNKLNIDPESLIPELPSPKDLRPFPTRCSTIYRGHEGRIRTLSIDPSGLWLATGSDDGSVRIWEILTGREVYKVQVVDPEAKSGDNVEAIEWNPDKTTGILAIAAGENIFLIVPPIFGFEVENTGRTKIEDGFGFDTYGNSKTSNLNVNSDDENDQPESTAVKSQVAQWHKASQKQAEKDIAVVITCRHTVKKLSWHRKGDYFVTVQPKSGHTSVLIHQLSKHLSQSPFKKSKGIVMDAKFHPFKPQLFVCSQRYVRIYDLSQQALVKKLMPGARWLSTIDIHPRGDNLLASSFDKRVLWHDLDLASTPYKTLRYHEMAVRKVAFHKKLPLFCSASDDSIIHVFHATVYDDMLKNPLLVPLKKLEGHKVVNSLGVLDLVWHPREAWLFSAGADNTARLWTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCQ4</accession>
    <name>C5DCQ4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">alcohol dehydrogenase (NADP(+))</fullName>
            <ecNumber evidence="9">1.1.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0B04972g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a primary alcohol + NADP(+) = an aldehyde + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15937"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15734"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:15938"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="10">
            <dbReference type="Rhea" id="RHEA:15939"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.2" evidence="9"/>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552003.1">
        <property type="nucleotide sequence ID" value="XM_002551957.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCQ4"/>
    <dbReference type="SMR" id="C5DCQ4"/>
    <dbReference type="FunCoup" id="C5DCQ4">
        <property type="interactions" value="583"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCQ4"/>
    <dbReference type="GeneID" id="8290841"/>
    <dbReference type="KEGG" id="lth:KLTH0B04972g"/>
    <dbReference type="eggNOG" id="KOG0023">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_20_2_1"/>
    <dbReference type="InParanoid" id="C5DCQ4"/>
    <dbReference type="OMA" id="GWGEQKF"/>
    <dbReference type="OrthoDB" id="1879366at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008106">
        <property type="term" value="F:alcohol dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006066">
        <property type="term" value="P:alcohol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05283">
        <property type="entry name" value="CAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000158">
        <property type="entry name" value="Zinc-binding alcohol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047109">
        <property type="entry name" value="CAD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42683">
        <property type="entry name" value="ALDEHYDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="12">
        <location>
            <begin position="14"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00050997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR21565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="360" mass="39726" checksum="45046DE108D44C04" modified="2009-07-28" version="1">MSYPEKFQGIAVQDHNDWTHPKKISYEPKKFDDYDVDVEIEACGICGSDTHCASGNWGPKSLPLVVGHEVIGRVVRVGPKCHTGVKVGDRVGVGAQVSSCLDCDRCNSDFEPYCAEFITTYSQPYPDGYVSQGGYASHIRAHEHFVIPIPEKIPTHLAAPLLCGGITVFSPLINNDCGPNKKVGIVGIGGIGHMGLIFAKALGAEVYAISRSSAKKEDAMKLGADHFIATKEEPDWEKKYASTLDLVIVCASSLTEINLEQLIKTMRIKGRIISICVPKETEELNLKPFQLSGITIGNSQLGSRKELIQLLQLVADKDLKIWVETLPISEESVHDAFVRMEKGDVRYRFTLTDFDKEFRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCU1</accession>
    <name>C5DCU1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0B05808p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0B05808g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552040.1">
        <property type="nucleotide sequence ID" value="XM_002551994.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCU1"/>
    <dbReference type="FunCoup" id="C5DCU1">
        <property type="interactions" value="164"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCU1"/>
    <dbReference type="GeneID" id="8290879"/>
    <dbReference type="KEGG" id="lth:KLTH0B05808g"/>
    <dbReference type="eggNOG" id="ENOG502QSUF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_673016_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCU1"/>
    <dbReference type="OMA" id="TRFHFAN"/>
    <dbReference type="OrthoDB" id="270970at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="CDD" id="cd08681">
        <property type="entry name" value="C2_fungal_Inn1p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037791">
        <property type="entry name" value="C2_fungal_Inn1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052981">
        <property type="entry name" value="Ingression_C2_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47052">
        <property type="entry name" value="CONSERVED SERINE PROLINE-RICH PROTEIN (AFU_ORTHOLOGUE AFUA_2G01790)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47052:SF3">
        <property type="entry name" value="INGRESSION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="2">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="291"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="298"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="308"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR21602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="387" mass="43118" checksum="0AC11302DF15C99F" modified="2009-07-28" version="1">MSTVSASAFGGANGLLEVYVSRARDLPNLRKLDKQSPFVRLRISHMTETSDVAFRGGQTPKFDYYAKFDLTPDVKPALAVEVFDDRSPPKLIGQCAVDLTPALYKDPEDGHDDWFPLSFGSEEAGEIYLELTFHPLIPTSRLQRHRNDGISAFEESLASRPPPPLPAKLPSFQLEKRHDEVTHNNTYSHASNLSQTLLSEPERISFGQSVSSKRMPNLDVSVASNSTFASQATNETHESHATVATSGTTEPLFAKLKQLKEKWNSIKNPGKDQQDHDKKVDLEALQKVVGVEPEEFNQDERGFRRVTDVSTQASKLVTQPPLPRLPDQSSRRTSIESRALPTSRPNSCINETSPKLPPLPRSPSSRPDLRTRTAPNSPVRRKPPPKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDA7</accession>
    <name>C5DDA7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0B09636p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0B09636g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class IV-like SAM-binding methyltransferase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552206.1">
        <property type="nucleotide sequence ID" value="XM_002552160.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDA7"/>
    <dbReference type="FunCoup" id="C5DDA7">
        <property type="interactions" value="1031"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDA7"/>
    <dbReference type="GeneID" id="8291049"/>
    <dbReference type="KEGG" id="lth:KLTH0B09636g"/>
    <dbReference type="eggNOG" id="KOG3925">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061859_0_0_1"/>
    <dbReference type="InParanoid" id="C5DDA7"/>
    <dbReference type="OMA" id="YGYHVRI"/>
    <dbReference type="OrthoDB" id="361029at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18086">
        <property type="entry name" value="HsC9orf114-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003750">
        <property type="entry name" value="Put_MeTrfase-C9orf114-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12150">
        <property type="entry name" value="CLASS IV SAM-BINDING METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12150:SF13">
        <property type="entry name" value="METHYLTRANSFERASE C9ORF114-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02598">
        <property type="entry name" value="Methyltrn_RNA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR21768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="389" mass="43070" checksum="8450173027F95019" modified="2009-07-28" version="1">MAPKREGGVVGKGHNETAKKSKLSKPAKYTENNKKAANFTTKAPKKKLKKTIKICSKTINYSLCIPVSVIDNCKNLEQATHVVYQIAKSAVLFNVGEIVVLDLGRAEDVPKESRHRFQMSPVLLVASLLQYFVTPPYLVKSVFKKEYQECFKVAAKLPRITALPFMRHLHEDNGRYREGLAVRMQRPAQGARPAKKKAYDQTKYVNVGKDSALELRGQLVPANVRVTVDLIDCKVVSPAEAYGDFVGAQASYGFHVRVAKTFADIFTQSPAPNGYTQTVWVNSGDFYFDPTTKKSLKLETKIPRIGKILKPTPEEVASGEAENPAHLLVVLGKWDHLKKSFARSREMFAGCEGAHQFFDGQLELPGSAPEANIGIHDSCMIAMTMLASL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDE5</accession>
    <name>C5DDE5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0C00374p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0C00374g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ZIP transporter (TC 2.A.5) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552244.1">
        <property type="nucleotide sequence ID" value="XM_002552198.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDE5"/>
    <dbReference type="FunCoup" id="C5DDE5">
        <property type="interactions" value="616"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDE5"/>
    <dbReference type="GeneID" id="8291102"/>
    <dbReference type="KEGG" id="lth:KLTH0C00374g"/>
    <dbReference type="eggNOG" id="KOG1558">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027089_0_2_1"/>
    <dbReference type="InParanoid" id="C5DDE5"/>
    <dbReference type="OMA" id="YNPGSFT"/>
    <dbReference type="OrthoDB" id="448280at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000006">
        <property type="term" value="F:high-affinity zinc transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071578">
        <property type="term" value="P:zinc ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004698">
        <property type="entry name" value="Zn/Fe_permease_fun/pln"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00820">
        <property type="entry name" value="zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040:SF32">
        <property type="entry name" value="ZINC-REGULATED TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040">
        <property type="entry name" value="ZINC/IRON TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="46"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="122"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="219"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="281"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="323"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="355"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR21806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="376" mass="41441" checksum="B7F3552CAB2C7D1D" modified="2009-07-28" version="1">MSGSEPWWQQYDPDTVTISDDSVPDSWKICTIDGVYFGGNSYSGSLGARISSIFVILFMSTFFTLFPVIAARSKRLKVPKYVYLFARYFGTGVIVATAFIHLLDPAYGEIGPQSCVGMSGNWSKYSFCPAIILFTVFSIFIVDLASDVYVKRRFGITHGHGDEIENAIVKRQEATNNNVDIESHNLGHSADSDDKSKKSYDVVSNASTEIVTQSFESQIGAFLILEFGVIFHSVMIGLNLGTTDDEFSTLYPVLVFHQSFEGLGIGARLSAIEFPKNKWWWPYALCVAYGLTTPICVAIGLGVRTTYDGNSYTVNVVSGVLDAISAGILMYTGLVELLARDFIFDENRTNDISKLLFMVNCTLWGAGLMALLGKWA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-06-28" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDJ3</accession>
    <name>TDA11_LACTC</name>
    <protein>
        <recommendedName>
            <fullName>Topoisomerase I damage affected protein 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">TDA11</name>
        <name type="ordered locus">KLTH0C01452g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TDA11 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552292.1">
        <property type="nucleotide sequence ID" value="XM_002552246.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDJ3"/>
    <dbReference type="SMR" id="C5DDJ3"/>
    <dbReference type="FunCoup" id="C5DDJ3">
        <property type="interactions" value="17"/>
    </dbReference>
    <dbReference type="GeneID" id="8291150"/>
    <dbReference type="KEGG" id="lth:KLTH0C01452g"/>
    <dbReference type="eggNOG" id="ENOG502S2SD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046807_0_0_1"/>
    <dbReference type="InParanoid" id="C5DDJ3"/>
    <dbReference type="OMA" id="ERTLNWD"/>
    <dbReference type="OrthoDB" id="4036304at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031388">
        <property type="entry name" value="Tda11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17084">
        <property type="entry name" value="TDA11"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0175">Coiled coil</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000410758" description="Topoisomerase I damage affected protein 11">
        <location>
            <begin position="1"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="197"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="263"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="332"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="125"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="39"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="59"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="114"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="200"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="211"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="283"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="343"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="354"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="366"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="379" mass="41694" checksum="0E1BAD23F7CC9004" modified="2009-07-28" version="1">MSKLDGFIEEHDRESNIDSGARLESTKPSPKVGSPAQRVRQNGVKSPILQTQATPAKPSEQEHPLKETPTRATAGGKMTRSNTLKRLSLIQPVISPETTPKEHRQQQATRNRSRSVVSVHSRSSSSASEQLADATKDVNALLQLLANKELELLETKHKIEELKKTLTQEEKALLRQTHELQDLKTQVGKALNSGIDEQIQQQSQSSHHSPNQRSLAVPTSHTPGNESRPAHAKKESVWTKPLSFLNQFDQLIQHELEKKLNWDDVASPEKTPGAESGTLSGRSASPASYNNGNSNNNNNNNSNRGRPNEDVLGNMSSSLWSFVSDVKTGLLGINEEEEEESAEAPAVASSGESNNQLRFVGSKNNSEVELKEYNASKRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-04-02" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDQ0</accession>
    <name>C5DDQ0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0C02794p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0C02794g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552349.1">
        <property type="nucleotide sequence ID" value="XM_002552303.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDQ0"/>
    <dbReference type="GeneID" id="8291210"/>
    <dbReference type="KEGG" id="lth:KLTH0C02794g"/>
    <dbReference type="eggNOG" id="ENOG502S6F0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1635701_0_0_1"/>
    <dbReference type="InParanoid" id="C5DDQ0"/>
    <dbReference type="OrthoDB" id="21418at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029196">
        <property type="entry name" value="HAPSTR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38645">
        <property type="entry name" value="CHROMOSOME 9, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38645:SF1">
        <property type="entry name" value="YALI0F12243P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15251">
        <property type="entry name" value="TAPR1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="101"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="162" mass="17749" checksum="F22553CF0182EE67" modified="2009-07-28" version="1">MDRLNSLEKSLPPERPATDQAIDKLNQQVTQEFKIAANAVTSLYRLSSERSSLSRHAGYAECLDDVLQQLESGRTAEELRQWCENRKHEIRGSAAAKTDSSAPTVVARTPAAPGTPASGVAAPLGSFASRDRRAKPNRASVAQADPERKIKKPRNWSNRSEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDU7</accession>
    <name>C5DDU7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription initiation factor TFIID subunit 8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0C03916g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552396.1">
        <property type="nucleotide sequence ID" value="XM_002552350.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDU7"/>
    <dbReference type="FunCoup" id="C5DDU7">
        <property type="interactions" value="272"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDU7"/>
    <dbReference type="GeneID" id="8291260"/>
    <dbReference type="KEGG" id="lth:KLTH0C03916g"/>
    <dbReference type="eggNOG" id="KOG4336">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030592_0_0_1"/>
    <dbReference type="InParanoid" id="C5DDU7"/>
    <dbReference type="OMA" id="NICISNF"/>
    <dbReference type="OrthoDB" id="2193813at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08049">
        <property type="entry name" value="TAF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037818">
        <property type="entry name" value="TAF8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019473">
        <property type="entry name" value="TFIID_su8_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46469">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46469:SF1">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10406">
        <property type="entry name" value="TAF8_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription factor TFIID subunit 8 C-terminal" evidence="9">
        <location>
            <begin position="183"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="407"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="360"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="427"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="468"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF10406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR21958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="570" mass="64711" checksum="9420982F90C12753" modified="2009-07-28" version="1">MTKSAPDSPSSSSSELKGVQLRHLPNLEEIPRTALDDPLRKLLEKAVAIQLKAVNSNVSISQFAFENLIILVEQMLSNMLVDLHKMVNIQRRHCISKKDLLLIIDGYNLTCADLMVELERSQYVRSHNLERVRKVREESEVVEAKEKTPLSEKELLESKHLEFFIQDKEILTLVPPTPKNTKYVPRWLPEFPPDHTYRFTSQFNRPITDERLMKRKLAEEADLSEKALIHLSKLSHEESPSEGADSDEIFKESQQDTQLIFGPAKKTKIAAINGASDLLRTLPQQNYNVEEYARNRVEIARRKVEEYERHQLLLQKGAFIKAAHLLSPFAKKRNCKQVEKEVRTLLHRSYIGLLKSAPLLREQRKREVELAEENRRLREEQIRQEREERLKNSKDYDVLDLDNLNEDPFFGGLGSSDTEDEKEPPQQEGTNKSGNTAPSATPEISDRANTMSADIPGRSVLDEAESQDLTREADMEFDSEKNKDDTSNEYSFQTAGNPRLSPGGPSSALHDFTSTPPALPSSDNREIHEGTPSTSAPQVPDNGEQQRQKAPEASVNVNDASAEPQEPRTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>C5DE14</accession>
    <name>C5DE14_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Protein kinase C-like 1</fullName>
            <ecNumber evidence="2">2.7.11.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="ordered locus">KLTH0C05478g</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.13" evidence="2"/>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552463.1">
        <property type="nucleotide sequence ID" value="XM_002552417.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DE14"/>
    <dbReference type="FunCoup" id="C5DE14">
        <property type="interactions" value="501"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DE14"/>
    <dbReference type="GeneID" id="8291330"/>
    <dbReference type="KEGG" id="lth:KLTH0C05478g"/>
    <dbReference type="eggNOG" id="KOG0694">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_54_1_1"/>
    <dbReference type="InParanoid" id="C5DE14"/>
    <dbReference type="OMA" id="QCTHLVP"/>
    <dbReference type="OrthoDB" id="63267at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004697">
        <property type="term" value="F:diacylglycerol-dependent serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009272">
        <property type="term" value="P:fungal-type cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20822">
        <property type="entry name" value="C1_ScPKC1-like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20823">
        <property type="entry name" value="C1_ScPKC1-like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11620">
        <property type="entry name" value="HR1_PKC-like_2_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05570">
        <property type="entry name" value="STKc_PKC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000101">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000103">
        <property type="entry name" value="Protein kinase C"/>
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    <dbReference type="FunFam" id="3.30.60.20:FF:000014">
        <property type="entry name" value="Protein kinase C"/>
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    </dbReference>
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        <property type="entry name" value="Protein kinase C"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011072">
        <property type="entry name" value="HR1_rho-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036274">
        <property type="entry name" value="HR1_rpt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037312">
        <property type="entry name" value="PKC-like_HR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02185">
        <property type="entry name" value="HR1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00742">
        <property type="entry name" value="Hr1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46585">
        <property type="entry name" value="HR1 repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51860">
        <property type="entry name" value="REM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16 18" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="REM-1" evidence="24">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="REM-1" evidence="24">
        <location>
            <begin position="100"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="20">
        <location>
            <begin position="183"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="22">
        <location>
            <begin position="407"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="22">
        <location>
            <begin position="474"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="837"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="23">
        <location>
            <begin position="1097"/>
            <end position="1164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="298"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="541"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="605"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="670"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="762"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="799"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="18">
        <location>
            <begin position="36"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="312"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="565"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="768"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="799"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="19">
        <location>
            <begin position="820"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="866"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00067241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="CAR22025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1164" mass="132248" checksum="C0ACD8F94C385914" modified="2009-07-28" version="1">MLTQVEQEIRRKIERELNIIQGASQLKKKTDNVMVIQKCNTNIREAQQNIQYLEETLKKLQISSDQVERNPSQQRASQVYGQLSTIAPDEHSFSRLDLMKYDCPSLSQRIQYMLQQLEFKLQVEQQYQEANEKLTKLYQIDGDQRSSSAAEGGSRESKQRINLLKKSLKKYQAINVDLDQFRHQGESSHATQPKFRRKQLTGVLSIGVTAIRDVDHVQSPLFSRKPDTFITIKIDDTVKAKTKPSRNGRWHESFNINVGKGNEVELTVYDRVNDQTVPVAQMWLLLSDIAEEIRKKKAEQSAPIDWNPAAGVTSSSEHSTKQANAGAENADRRLGATSEDIASPSQEAALSQPVTTSLWFVLEPAGQILLNLGFSKSSAPTDKHLIGGLHRHGAIINRKEEVYEQHGHHFVQKSFYNIMCCAYCGDFLRYSGFQCQDCKFLCHKKCYNHVVTKCIAKSSSDTDPDEAKLNHRIPHRFEPISNRGTKWCCHCGYILPWGKKNLRKCSECGIMCHVQCAHLVPDFCGMSMGMANKILTAIQDTKRSQEQRQKLQVSPPKHQYPGAELSKKTSRVSERSFETKQLPVSPTQKNLSDVRLGRIDVAAEDQRSDKQRFTETAQKTKIKPTASETERNGVINERLNNFIEENADYEAFTQGAGSLQLQDLDTKTRSLDPSLASGLASDKPDLSTQENDLSRDNETSILLEQQRDAQLERARDHQGFTEFDDSYDDAMVPLETKRSSFNNTMSTQVDPGAQLPQKWQITPEPEEIPQKTEEHTTKAYKEQRHQDLSDAKLVQITPQQSADALTKSQQSSPEAPQTGGRHKRKAPKRRKISLDDFVLLKVLGKGNFGKVLLAESKNNERLCAIKVLKKDHIIKNHDIESARAEKKVFLLATKAKHPFLTNLYCSFQTENRIYFAMEFIGGGDLMWHVQNQRLSVRRAKFYAAEVLLALKYFHDNGVIYRDLKLENILLTPEGHIKIADYGLCKDEMWFGNKTSTFCGTPEFMAPEILKEQEYTKAVDWWAFGVLLYQMLLCQSPFSGDDEDEVFNAILTDEPLYPIDMAGDIVQIFQGLLTKDPERRLGAGPRDAAEVMAEPFFRNINFDDILHKRVQPPYIPEIKAADDTSYFEKEFTSAPPTLTPLPSVLSSSLQEEFRGFSFMPDDLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DE34</accession>
    <name>C5DE34_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0C05940p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0C05940g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VAMP-associated protein (VAP) (TC 9.B.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552483.1">
        <property type="nucleotide sequence ID" value="XM_002552437.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DE34"/>
    <dbReference type="FunCoup" id="C5DE34">
        <property type="interactions" value="594"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DE34"/>
    <dbReference type="GeneID" id="8291351"/>
    <dbReference type="KEGG" id="lth:KLTH0C05940g"/>
    <dbReference type="eggNOG" id="KOG0439">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072622_1_0_1"/>
    <dbReference type="InParanoid" id="C5DE34"/>
    <dbReference type="OMA" id="MIPEHEV"/>
    <dbReference type="OrthoDB" id="264603at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033149">
        <property type="term" value="F:FFAT motif binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090158">
        <property type="term" value="P:endoplasmic reticulum membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061817">
        <property type="term" value="P:endoplasmic reticulum-plasma membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000535">
        <property type="entry name" value="MSP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008962">
        <property type="entry name" value="PapD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016763">
        <property type="entry name" value="VAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10809:SF6">
        <property type="entry name" value="AT11025P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10809">
        <property type="entry name" value="VESICLE-ASSOCIATED MEMBRANE PROTEIN-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00635">
        <property type="entry name" value="Motile_Sperm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019693">
        <property type="entry name" value="VAMP-associated"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49354">
        <property type="entry name" value="PapD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50202">
        <property type="entry name" value="MSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="222"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="MSP" evidence="8">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="133"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="133"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="147"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="200"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR22045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="244" mass="25891" checksum="B965958C89809E3F" modified="2009-07-28" version="1">MIEIKPSVLEYKPPLTAQATEYVTVTNNTDQAVAFKVKTTAPKFYCVRPNAALVAPGEQVQVQVILLGLAEEPAPDFKCKDKFLVITLPAPYDLGASSVTEAWPQLESEFKQHAVSKKIKVKYLLGDEAPVRAEESSAAVKREEPVGAKAAGASSSAPQAEEPQETKAVTLKPEEPAQKPALGAVQEDVKPEAAPEPEPVSKAAAATAGGASEKHVAQKQEAAINPAIIALVLLIALVLGWKFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DE48</accession>
    <name>C5DE48_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">LSM complex subunit LSM4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LSM4</name>
        <name evidence="12" type="ordered locus">KLTH0C06248g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Binds specifically to the 3'-terminal U-tract of U6 snRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">LSm subunits form a heteromer with a doughnut shape.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552497.1">
        <property type="nucleotide sequence ID" value="XM_002552451.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DE48"/>
    <dbReference type="FunCoup" id="C5DE48">
        <property type="interactions" value="303"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DE48"/>
    <dbReference type="GeneID" id="8291365"/>
    <dbReference type="KEGG" id="lth:KLTH0C06248g"/>
    <dbReference type="eggNOG" id="KOG3293">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099537_0_0_1"/>
    <dbReference type="InParanoid" id="C5DE48"/>
    <dbReference type="OMA" id="VACDAWM"/>
    <dbReference type="OrthoDB" id="747253at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097525">
        <property type="term" value="C:spliceosomal snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01723">
        <property type="entry name" value="LSm4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000064">
        <property type="entry name" value="U6 snRNA-associated Sm-like protein LSm4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034101">
        <property type="entry name" value="Lsm4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027141">
        <property type="entry name" value="LSm4/Sm_D1/D3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23338">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 9" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="11">
        <location>
            <begin position="2"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="78"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="102"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="126"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR22059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="175" mass="19521" checksum="52C28D5AF50EF847" modified="2009-07-28" version="1">MLPLYLLTNAKGQQLVIELKNGETIVGQLTNVDNWMNLTLTDVTHTSASDTVKLSEIYVRGNFIKYIKLQDDVIEKVKQSNAQQQSQGHHQGRDFRRRQGGGRRDGDKRFHSGNPRRGDGRDSYHNNGGNRRFNNNYNRAEGGNSGNNAMGGFVRHHQTSAQPGTFLGGQGQQNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>C5DE79</accession>
    <name>C5DE79_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0C06974p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0C06974g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552528.1">
        <property type="nucleotide sequence ID" value="XM_002552482.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DE79"/>
    <dbReference type="FunCoup" id="C5DE79">
        <property type="interactions" value="121"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DE79"/>
    <dbReference type="GeneID" id="8291397"/>
    <dbReference type="KEGG" id="lth:KLTH0C06974g"/>
    <dbReference type="eggNOG" id="ENOG502S9CX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_163517_0_0_1"/>
    <dbReference type="InParanoid" id="C5DE79"/>
    <dbReference type="OMA" id="HSYELFR"/>
    <dbReference type="OrthoDB" id="4033270at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020366">
        <property type="entry name" value="Ies5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17335">
        <property type="entry name" value="IES5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="114" mass="13080" checksum="44A796EAEC7AC100" modified="2009-07-28" version="1">MTSSKIPVPSKEYAKQLSRREELTKQESSLKREYTTMLRKMASVMAVLQELDDGQEASQQTISESALLKVPELKQYSQLLHDIDSKALEDIEIPEVLKDSYALYKQSPLLYKDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DED0</accession>
    <name>C5DED0_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Kinase</fullName>
            <ecNumber evidence="4">2.7.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0C08184g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the inositol phosphokinase (IPK) family.</text>
    </comment>
    <dbReference type="EC" id="2.7.-.-" evidence="4"/>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552579.1">
        <property type="nucleotide sequence ID" value="XM_002552533.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DED0"/>
    <dbReference type="FunCoup" id="C5DED0">
        <property type="interactions" value="227"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DED0"/>
    <dbReference type="GeneID" id="8291452"/>
    <dbReference type="KEGG" id="lth:KLTH0C08184g"/>
    <dbReference type="eggNOG" id="KOG1620">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004422_0_0_1"/>
    <dbReference type="InParanoid" id="C5DED0"/>
    <dbReference type="OMA" id="RVRIGWQ"/>
    <dbReference type="OrthoDB" id="2573163at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000824">
        <property type="term" value="F:inositol-1,4,5,6-tetrakisphosphate 3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008440">
        <property type="term" value="F:inositol-1,4,5-trisphosphate 3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032958">
        <property type="term" value="P:inositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.160">
        <property type="entry name" value="Inositol polyphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005522">
        <property type="entry name" value="IPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038286">
        <property type="entry name" value="IPK_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12400">
        <property type="entry name" value="INOSITOL POLYPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12400:SF21">
        <property type="entry name" value="KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03770">
        <property type="entry name" value="IPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="48"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="97"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="172"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="230"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="365"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="419"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="474"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="48"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="184"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="234"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="268"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="419"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="479"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="493"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="504"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR22141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="886" mass="101099" checksum="EFBA1962CD6CBBB4" modified="2009-07-28" version="1">MGSANEDNTRRGLSSLSTHLRDLNVFDKQKLPPVIHGRKASTYLRIFQDDEHTHNREPQGAVTKPEDARAEPVSWKMPFHQTKTLLHGHVHEPRLSLGRRHSFSSSDVHHKSRRGSLKERNTQPFHGYRPPRLPKKAQVGSGSQRYPLHSAEGLQLHHESDTIPEGACPQLAQAQQRPHALAENSETGEVDSQGNKAKTLALKPISSATYYPHQTHNELADGTAPVTLAHSAPSVPQRTPLKTHQSQPGVPQTTRKIESRQQGSKSEDESEHDEEEVYPLAVELQPFNNKVGGHTAIFRFSERAVCKALVSRENMWYENIEQQHKELLQFIPRYIGVLNVRQHFNSKEEFLQEVEREKKAKIIKKQQKMDSLSVDGPSQKETRCATPPLAMKNPEVVLDDNKHIIPDSLWYKYSHSPESAPSDSFLSSHSMEDRSSRGWDSGSTVVNTKLQELVLKEVFAPTKRCAHARVSSLGERQLPQGSVSSNNLTSFNNDRRNSEELKQPDSTSPSSPMLNKSAKVSIANAIDSHSSVLDLKQFNERKLAQIDAETKTLSQQKPDETLRSSSPFHKFAEGQTDDEGVFTMEEDCPRTKEEPPNESVLFEENSHTIVSKFILLEDLTRKLDKPCVLDLKMGTRQYGVSANKFKRLSQNKKCRQTTSRKLGVRICGLKIWSKDYYIKRDKYFGRRVKIGWQFARVLARFLYNGEQISSIVSQIPRVISQLDKLYAEISKLKGYRLYGSSLLLIYDGNGSSSKKCEVKVNLIDFAQCVTSDDVATSWDSFRIPPKSPETEDRGFLRGVRSLKFYLQSLWNYLTGDSPLLFDDALNEYIQCNKESFEKPWDWLDNFDQESEFEFDDAESELRKKWRKYELIFDVEPRYMDDAEISE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEH3</accession>
    <name>C5DEH3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">KLTH0C09240p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0C09240g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Cooperates for the ATP-dependent vacuolar transport of bilirubin and glutathione conjugates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552622.1">
        <property type="nucleotide sequence ID" value="XM_002552576.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DEH3">
        <property type="interactions" value="172"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEH3"/>
    <dbReference type="GeneID" id="8291500"/>
    <dbReference type="KEGG" id="lth:KLTH0C09240g"/>
    <dbReference type="eggNOG" id="KOG0054">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_27_3_1"/>
    <dbReference type="InParanoid" id="C5DEH3"/>
    <dbReference type="OMA" id="KTWIMAF"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18579">
        <property type="entry name" value="ABC_6TM_ABCC_D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18603">
        <property type="entry name" value="ABC_6TM_MRP1_2_3_6_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000565">
        <property type="entry name" value="ABC bile acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000020">
        <property type="entry name" value="ABC metal ion transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000013">
        <property type="entry name" value="ABC transporter C family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000997">
        <property type="entry name" value="Multidrug resistance-associated protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044746">
        <property type="entry name" value="ABCC_6TM_D1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF443">
        <property type="entry name" value="MULTIDRUG-RESISTANCE LIKE PROTEIN 1, ISOFORM I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="34"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="113"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="174"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="284"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="334"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="435"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="523"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="963"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1013"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1091"/>
            <end position="1112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1118"/>
            <end position="1138"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="297"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="618"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="977"/>
            <end position="1261"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="1298"/>
            <end position="1541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00053425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR22184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1546" mass="173605" checksum="93A20D14EC16FF25" modified="2009-07-28" version="1">MATYQQTHTIKRQFMGQKDCAYGLRPYPDHKTNALNPCFLSAVSVGHASFFAIIGVFQLARLARERRVPPNFRYVSSWKSISTRHLLHLSNIGLQSVLFICALSVCGPDTSQITKWGLVSQLLYVALISLPSQVIEFYKSTASLAGQLFYYLTQIFLLGYQLGQRISHYPDERFVIVTGSYGAFLEVALLVNAISIFLYDLLSYKPSQELLNYYEEHKMYQECNLLQNLTFTWMNKLIGQVYRDGEIKDPHNMPLPPINLSIKEKAGALGASWAKENWTGRNSLLWAIMNTFGKTIIIAISIEVVKDFMTILQPQLLRLFIEEFNQDRERTHPLINAVFIAFALFLMKLVSTCLSNQFFIMIFEAGMGIRGALMTMLYQKSLKLSSEAREGKTAGDIMNMMAVDVLRIQRFFETSQELVGSPIALLTTLVSLYTFLGYSTAGGVVIMAVMFPINSYLSRKIKVLIKKQMEYKDARIKTVTEILNSVKTVKLYAWEEPMLKRLDHVRNELELESFKKIAVMNNLTLFAWNCVPILVASSTFLIYALVMDVPLTPQIVFPSLSLFDILNDCIYTIPRTIINFIETGVSMGRLKDFLLAKELDKSFIEFESLPDDPNVPVIEVHNATFLRNLPTKATSEENYDEEAMIESSRVALKNIDGFQALKGQLVCVVGRVGAGKSTFLHALLGQLPCISSSHDQATPKMHFRCDSVALCSQQAWILNASVKYNVLFGHKYDEASYKATIEACQLLPDLGILADGDETLVGEKGISLSGGQKARISLARAVYSRSDVYLLDDILSAVDAEVCKRIIDHVLSRKTGLLKNKTVILTTNSISVLKHSQSIYALENGEIVERGSFDEIMSKDAESKLKTLIAEFGSDSSAVKDDEPIPEQKPPVDLQEVEDDALEEEINPEVPFELDNAFSSDFRSRKASMATLRARKIIDLNADTRKTAQKAEKKEKGRVKTKVYVAYLKACGILGAVLFLVFIGFSRVLLVGENFWLKHWSEKNEKNGENRNAVFFVGIYVLISLGAAFFNSARSIVLLLVCSIRASRKLHDSMAVAVLRSPMSFFETTPVGRIINRFSSDMNSVDDNVQYVISFFLISILDYVVVVVIIGYQVPLFLLVNAALLGIYLYYQVFYVTLSRELKRLMSTSFSPIMSMLSETLAGHMVINAFNHFSRFDYLNIEAVQFNINCVFNFRSTNRWLSIRLESIGALMILTTALLSLATTTGDKALSTGMVGLLMSYALQVTNKLMWIVRMSVQLETNVVSVERIVEYCDLPPEAPPVIEDCRPEKNWPAQGHVVFKDYSARYRENLDPVLKKLNVEINPQEKIGIVGRTGAGKSTLSLALFRILEPCGGTIVIDGVDITKIGLADLRSHLSIIPQDAQAFEGSVRSNLDPFEQYSDDEIWRALELSHLKPHILKMANEEGPEKTKNLLETKISENGSNLSVGQRQLLCLSRALLNRSRILVLDEATAAVDSETDRLIQETIRAEFKDRTILTIAHRIDTVMDSDKIMVLDQGEVKEFDSPSNLLADKNTIFYNLCSQGGYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEL4</accession>
    <name>C5DEL4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0C10208p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0C10208g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GET4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552663.1">
        <property type="nucleotide sequence ID" value="XM_002552617.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEL4"/>
    <dbReference type="FunCoup" id="C5DEL4">
        <property type="interactions" value="406"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEL4"/>
    <dbReference type="GeneID" id="8291543"/>
    <dbReference type="KEGG" id="lth:KLTH0C10208g"/>
    <dbReference type="eggNOG" id="KOG3024">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046061_0_1_1"/>
    <dbReference type="InParanoid" id="C5DEL4"/>
    <dbReference type="OMA" id="VKWSVKA"/>
    <dbReference type="OrthoDB" id="10252405at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072380">
        <property type="term" value="C:TRC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045048">
        <property type="term" value="P:protein insertion into ER membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000615">
        <property type="entry name" value="Golgi to ER traffic protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007317">
        <property type="entry name" value="GET4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12875">
        <property type="entry name" value="GOLGI TO ER TRAFFIC PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12875:SF0">
        <property type="entry name" value="GOLGI TO ER TRAFFIC PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04190">
        <property type="entry name" value="GET4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="303" mass="34692" checksum="BAAA389E09842515" modified="2009-07-28" version="1">MSANVKLGRTLARFHARIEAGDYYEAHQTLRTIANRYVRSKNYREAIDLITEGAQALLGAKQGGSATDLIFYLLEVYNVAEVKVEDVSVSRLVRLLALLDAEEPNMKDVITGMNNWSVKFGNCKFGDPYLHSVIGSKLVEGGHVYEAERYLTLGTHDSLDKYLDLIWEWYLQSGDREAVGDFFSRLIFNYLFIGNVQNAEQAKQKFLAKFIENFNPKYELIDRGEFKTYCFEDYPELNFLQLLPYSCQTKNADLFDFLKKHYAGSSERYGSELDYLGQEYFGIVVQKPMNFMQDILAGILGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEV8</accession>
    <name>C5DEV8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0D10164p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0D10164g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553151.1">
        <property type="nucleotide sequence ID" value="XM_002553105.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEV8"/>
    <dbReference type="FunCoup" id="C5DEV8">
        <property type="interactions" value="621"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEV8"/>
    <dbReference type="GeneID" id="8295390"/>
    <dbReference type="KEGG" id="lth:KLTH0D10164g"/>
    <dbReference type="eggNOG" id="ENOG502QRT9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008791_4_0_1"/>
    <dbReference type="InParanoid" id="C5DEV8"/>
    <dbReference type="OMA" id="IQEQQIH"/>
    <dbReference type="OrthoDB" id="3366990at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003691">
        <property type="term" value="F:double-stranded telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010833">
        <property type="term" value="P:telomere maintenance via telomere lengthening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11660">
        <property type="entry name" value="SANT_TRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000137">
        <property type="entry name" value="MYB DNA binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013867">
        <property type="entry name" value="Telomere_rpt-bd_fac_dimer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052833">
        <property type="entry name" value="Telomeric_DNA-bd_trans-reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47807">
        <property type="entry name" value="PROTEIN TBF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47807:SF1">
        <property type="entry name" value="PROTEIN TBF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08558">
        <property type="entry name" value="TRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH myb-type" evidence="5">
        <location>
            <begin position="362"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="310"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="446"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="310"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="326"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="451"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="470"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR22713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="513" mass="57414" checksum="EFA3694BACE5FD46" modified="2009-07-28" version="1">MSEALPAKDTDPLKSDDIKTGKVIAELPPRTQLNINSIGLLDNVSTQLLKLLMTSSIETLIGFTSAQSDGYQSEVFEMLFALFKQVKKVYTSAPLLVVQDIAPGLWLEDEKCPYILKNQENLVLAAIRKANIVTYLLSIMGRLQYGFLFLDESFIEIFCPVAGDLPFEAERALTTSLGRLLKPQAVLYLNLKTHAYISAIEPYRDNAEALSRVREEILDRIFAKNMEQFILAKRRMRSSKLSPSEEDFVKRCVHRREKLQSQDSLDLLVAEYDWLEFFKEIFFYLQRNISVLVWGKKGCDFASPGSDVSASDINADQPMSSLNNGSAESSRASSEPPVGKPLSSSSSLRPSPAPLRDAASAKKLKQKRLWNKEEEGALIEALKVHGPAWSKILELHGAGGSLSESLKNRTQVQLKDKARNWKMYFLKGGLPVPDYLAKVTGDLERDEKSKQRFKNKTKKQAPTKTIGNVEKPHEPTQDLRKVANMSIVAQAGRSEFPIEEANASEMDPQLQAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEW9</accession>
    <name>C5DEW9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0D10406p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0D10406g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Exerts its effect at some terminal stage of cytochrome c oxidase synthesis, probably by being involved in the insertion of the copper B into subunit I.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
            <orientation evidence="2">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553162.1">
        <property type="nucleotide sequence ID" value="XM_002553116.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEW9"/>
    <dbReference type="FunCoup" id="C5DEW9">
        <property type="interactions" value="557"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEW9"/>
    <dbReference type="GeneID" id="8295401"/>
    <dbReference type="KEGG" id="lth:KLTH0D10406g"/>
    <dbReference type="eggNOG" id="KOG2540">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045000_0_1_1"/>
    <dbReference type="InParanoid" id="C5DEW9"/>
    <dbReference type="OMA" id="YRIFCQS"/>
    <dbReference type="OrthoDB" id="1704689at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.370.10:FF:000001">
        <property type="entry name" value="COX11 cytochrome c oxidase assembly homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.370.10">
        <property type="entry name" value="Ctag/Cox11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00155">
        <property type="entry name" value="CtaG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023471">
        <property type="entry name" value="CtaG/Cox11_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007533">
        <property type="entry name" value="Cyt_c_oxidase_assmbl_CtaG"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003465">
        <property type="entry name" value="PRK05089.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21320:SF3">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX11, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21320">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04442">
        <property type="entry name" value="CtaG_Cox11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110111">
        <property type="entry name" value="Ctag/Cox11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="62"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR22724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="254" mass="28747" checksum="1B6EB84D70B432A4" modified="2009-07-28" version="1">MLHKLLFRAASRSIPARRLFSAGPMCLKNQSQIDISKLTQKEIQQLRSLKFQRDRQFKDRTAAYYFTSIAVLFLGLAYAAVPLYRAICARTGFGGIPITDKRKFTDDKLIPVESGKRIRVSFTSEVSQILPWKFVPEQREVHVLPGETALAFYKARNTSDKDIIGMATYSITPGDAAPYFNKIQCFCFEEQRLAAGEEIDMPVFFFIDPDFATDPRMRNIDDIVLHYTFFRAHYANGGAVSESANPQVMDSGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFJ4</accession>
    <name>C5DFJ4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0D15576p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0D15576g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SDS22 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553387.1">
        <property type="nucleotide sequence ID" value="XM_002553341.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFJ4"/>
    <dbReference type="FunCoup" id="C5DFJ4">
        <property type="interactions" value="371"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFJ4"/>
    <dbReference type="GeneID" id="8295632"/>
    <dbReference type="KEGG" id="lth:KLTH0D15576g"/>
    <dbReference type="eggNOG" id="KOG0531">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044236_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFJ4"/>
    <dbReference type="OMA" id="EVWASYN"/>
    <dbReference type="OrthoDB" id="266138at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000055">
        <property type="entry name" value="Protein phosphatase 1 regulatory subunit 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025875">
        <property type="entry name" value="Leu-rich_rpt_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050836">
        <property type="entry name" value="SDS22/Internalin_LRR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46652">
        <property type="entry name" value="LEUCINE-RICH REPEAT AND IQ DOMAIN-CONTAINING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46652:SF3">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12799">
        <property type="entry name" value="LRR_4"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR22949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="344" mass="39338" checksum="EDAE9EBB4D1FE62B" modified="2009-07-28" version="1">MASNNLANEPADEKCEDQSHLNIGSEIVPDPNIDHIEADAELTQDLPDDTEAIDLVHLRIRSMEDLGLQRFTKLRSLCLRQNLIESMSEVDVLPPETMVDIDLYDNKIKHISSNIGKLTNLESLDLSFNKIKHIKNLEALVKLENLFFVQNKISKVENLGSLKALRNLELGGNQIHEIEEDSLKGLTNLEEIWLGKNMITRLNNLHHLTQLRILSIQSNRLTKIEGLENLTNLEELYLSHNYIKKIEGLDKNMKLTTLDITSNKITKVENVHHLTQLTDLWLSFNQIDQSFESLGEELKELPAFETIYLEGNPIQTKNETTYRRKLVLNLGSSLQKIDATYIRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFZ8</accession>
    <name>C5DFZ8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0D01232p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0D01232g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552778.1">
        <property type="nucleotide sequence ID" value="XM_002552732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFZ8"/>
    <dbReference type="STRING" id="559295.C5DFZ8"/>
    <dbReference type="GeneID" id="8294998"/>
    <dbReference type="KEGG" id="lth:KLTH0D01232g"/>
    <dbReference type="eggNOG" id="KOG2508">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017405_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFZ8"/>
    <dbReference type="OMA" id="PASWWHE"/>
    <dbReference type="OrthoDB" id="415358at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041667">
        <property type="entry name" value="Cupin_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12461">
        <property type="entry name" value="HYPOXIA-INDUCIBLE FACTOR 1 ALPHA INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12461:SF100">
        <property type="entry name" value="JMJC DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13621">
        <property type="entry name" value="Cupin_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="JmjC" evidence="2">
        <location>
            <begin position="237"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR22340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="536" mass="59641" checksum="83DDF1A7AE43E0C5" modified="2009-07-28" version="1">MSKRPLNTNVIDGSKRPSSLNSEICNSYPGYCPGFGSVTVDEINVPSSKEEIDLFYQNYVLRRRPCKFKGVSSKEVLSEPLSPLNLVKTLPEQTILQVERKVQGGFGSGEKRLKMTLGELVERLRSGDEQLYLTTQYAEDDPDRDDASDVGENTEEYAEDDDDNEDDTKETDGGFGSAGDISDAGSFDFSNAHDDFEDLNGAESMDGESHDSKDVIAQLSTGPLTQFEADARVKELYQPPMDNLIEQLPETPSFLNSLIPQQINLWVGSSGNIGSSDKFLEGFDPSAPKLGLGRAIPGGGSSSGLHHDHADNIYVPISGRKRFTLFSPRDAAKMYTVGTIRKVYDSGVIDYARCETAPFWRTLRDDGAIEAEVAKNELEFNSSLTADQRQNLERVIEADSVCSSSIDSKLDPPSFSSIIPTVVHLDKVKDEGLRAEIKKVALEKWPMFFEANRLTVDLRPGEMLYLPTGWFHEVTSFGNEQTASADDRIHVAVNYWFIPPNGSSQDQLYAHEDQYWQKDYERTKAALEHARFCSKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>C5DG49</accession>
    <name>C5DG49_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glutathione synthetase</fullName>
            <shortName evidence="9">GSH-S</shortName>
            <ecNumber evidence="9">6.3.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0D02398g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>gamma-L-glutamyl-L-cysteine + glycine + ATP = glutathione + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13557"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58173"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9 11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="9 11">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the eukaryotic GSH synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.3" evidence="9"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552829.1">
        <property type="nucleotide sequence ID" value="XM_002552783.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DG49"/>
    <dbReference type="FunCoup" id="C5DG49">
        <property type="interactions" value="937"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DG49"/>
    <dbReference type="GeneID" id="8295049"/>
    <dbReference type="KEGG" id="lth:KLTH0D02398g"/>
    <dbReference type="eggNOG" id="KOG0021">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025152_2_1_1"/>
    <dbReference type="InParanoid" id="C5DG49"/>
    <dbReference type="OMA" id="NGLVMYP"/>
    <dbReference type="OrthoDB" id="2020073at2759"/>
    <dbReference type="UniPathway" id="UPA00142">
        <property type="reaction ID" value="UER00210"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043295">
        <property type="term" value="F:glutathione binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004363">
        <property type="term" value="F:glutathione synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.50:FF:000002">
        <property type="entry name" value="Glutathione synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1760">
        <property type="entry name" value="Glutathione synthase, substrate-binding domain superfamily, eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1080.10">
        <property type="entry name" value="Glutathione Synthetase, Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005615">
        <property type="entry name" value="Glutathione_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014042">
        <property type="entry name" value="Glutathione_synthase_a-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014709">
        <property type="entry name" value="Glutathione_synthase_C_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014049">
        <property type="entry name" value="Glutathione_synthase_N_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037013">
        <property type="entry name" value="GSH-S_sub-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004887">
        <property type="entry name" value="GSH_synth_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01986">
        <property type="entry name" value="glut_syn_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11130">
        <property type="entry name" value="GLUTATHIONE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11130:SF0">
        <property type="entry name" value="GLUTATHIONE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03917">
        <property type="entry name" value="GSH_synth_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03199">
        <property type="entry name" value="GSH_synthase"/>
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        <property type="entry name" value="GSHase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9" id="KW-0317">Glutathione biosynthesis</keyword>
    <keyword evidence="3 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="8 9 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 9 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutathione synthase substrate-binding" evidence="13">
        <location>
            <begin position="211"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="145"/>
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        <ligand>
            <name>Mg(2+)</name>
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        </ligand>
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            <begin position="147"/>
            <end position="150"/>
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        <ligand>
            <name>substrate</name>
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            <begin position="221"/>
            <end position="223"/>
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        <ligand>
            <name>substrate</name>
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    <feature type="binding site" evidence="10">
        <location>
            <position position="227"/>
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        <ligand>
            <name>substrate</name>
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    <feature type="binding site" evidence="12">
        <location>
            <begin position="278"/>
            <end position="281"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
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            <position position="317"/>
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        <ligand>
            <name>ATP</name>
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        <ligand>
            <name>ATP</name>
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            <position position="379"/>
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        <ligand>
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            <position position="386"/>
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            <name>substrate</name>
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            <position position="462"/>
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        <ligand>
            <name>ATP</name>
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        <location>
            <position position="468"/>
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        <ligand>
            <name>ATP</name>
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    <feature type="binding site" evidence="12">
        <location>
            <begin position="471"/>
            <end position="472"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR001558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001558-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001558-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR001558-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF03199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR22391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="484" mass="54245" checksum="6760AA15DF154F7E" modified="2009-07-28" version="1">MVGEYPKFPQFSRSEISEQLVPEIRHWALTNGLVMYPPNPNFVQGAVAPTTLYPTPFPRQSFDQAISVQTLYNGLYAKIAQDQDGWLSKEIESLAEFDPEFTGRLWNLYVQAKEKGISQKLALGVFRSDYLLDVGNLEVKQVEFNTVSVSFGGLSTRVGQLHKFLNDSGRYSGGSGRPFYREEIPVSDSAFLLAKSLADAAHHFQASNDTTIVAFVVQKGERNVFDQRILEYNLFEHFGIKSVRLTIHEVNTKTFVDPDTSRIYYKPSGEEISVVYFRAGYSPSDFVEEQDWQNRLTLETSYAIKAPNLLTQLAGAKKIQQLLTDESILQCFISDASARRKLGSSFVKIHPMDNSEAGLSARKLALKSPERFVLKPQREGGGNNIYKGDIPAFLRSIPEQDWSGYILMELIKPKPTTENFVVKADQFAQEPILSELGIFGYVLFDESTIYLNEYAGWLLRSKSSSSDEGGVAAGFGCVDSVVLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5DG92</accession>
    <name>C5DG92_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0D03388p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0D03388g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SAPS family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002552826.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DG92"/>
    <dbReference type="FunCoup" id="C5DG92">
        <property type="interactions" value="831"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DG92"/>
    <dbReference type="GeneID" id="8295094"/>
    <dbReference type="KEGG" id="lth:KLTH0D03388g"/>
    <dbReference type="eggNOG" id="KOG2073">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003676_2_0_1"/>
    <dbReference type="InParanoid" id="C5DG92"/>
    <dbReference type="OMA" id="HAYIACE"/>
    <dbReference type="OrthoDB" id="295029at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019903">
        <property type="term" value="F:protein phosphatase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007587">
        <property type="entry name" value="SAPS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12634:SF8">
        <property type="entry name" value="FIERY MOUNTAIN, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12634">
        <property type="entry name" value="SIT4 YEAST -ASSOCIATING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04499">
        <property type="entry name" value="SAPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="31"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="146"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="548"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="743"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="809"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="35"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="51"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="65"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="147"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="173"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="191"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="743"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="838"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="916"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="943"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="964"/>
            <end position="984"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR22434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1022" mass="113964" checksum="DFBE0F108625DF33" modified="2009-07-28" version="1">MSGSFWKFGQNYASESPLSKLLNRAFIKVSSEEGAQDKQQDNDSQEKINRLLASPGGSSSDGSESLEKSDADAEHGSADQNNALPDSEAEYRDYEPNMEVLDELLDDEELYTELMCSNFKLLIFFKYPQVLAKLVDYVTNEHVLSESLEESEEEDEKPISDAPKSGADDSEEPQNKESLGGDKNDREPMSDQLSVSSNETSITLPPDSEEQSDSRRARMAAEILSADVWPISSAFMDHEELLGRLWAMLDHPAPLSIVASTYFMKINERLLDMDISGMLHFILAQDDLVDRFLKHTDNPPLMDYLLKVISTDKPDTSTGVIEKLGSQELIPKLLDHLAAGHSSSTKSAAGDFLKALITISANSNNEIASAIGPNGLTRELVSPPMVEKLMNIMLSGGTSLSNGVGIIIELIRKNNSDYDFVQVMYTTLETHPPNDRDPIYLGYLIKCAAEHLPEFNKMLVETELPPLETPFGSIEPLGFERFKICELVAELLHCSNMTLLNEPSGGSIVAERDAVREKILQQEKSEGSHKKDENEDVLQKMSDLRIAATNSENSSDEDGIQTKEDADAAGEEIQSEGSSENELSEKSLRENPVVGDQLKIALQDNRVITSILEMFFDFPWNNFLHNVVFDIVQQIFNGPLKTGFNRFLIADLFTGAKLTHMIMDGDRKCEDYENETGLRLGYMGHLTLIAEEVAKFAAYVDEMKITFSNSAIVESLNEPRWKEYTETVLADTRENYSSVLGDFEEEEDDHEDPDIEDENPGGKTSVTGNDEGDGYRDFDNGAGSTGNYTEYDSVDNPRYYQYEDEHGNKTELHLNPDIDYDIGPGTFSENNAQELEDHDITEGGKDGKNKFSNYMSRQLSKDYNTSSSSDDDDEDIWGSSAPGSAQSSEFLKHGSKAGSSEFKDSGAFNPSHFGLEDDDDDYMDPNDDGQSYAKPHHPLYDSMLSQGKSENQLYYDQGDASLAENDDEDSDDSDKEDAGDEDDQYSLCRTSSKDNMAWDHAEQNRIINTAQYRQNQQELADK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DG98</accession>
    <name>C5DG98_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GPI-anchored wall transfer protein</fullName>
            <ecNumber evidence="8">2.3.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0D03520g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">A acetyltransferase, which acetylates the inositol ring of phosphatidylinositol during biosynthesis of GPI-anchor.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Endoplasmic reticulum membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the PIGW family.</text>
    </comment>
    <dbReference type="EC" id="2.3.-.-" evidence="8"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552878.1">
        <property type="nucleotide sequence ID" value="XM_002552832.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DG98"/>
    <dbReference type="FunCoup" id="C5DG98">
        <property type="interactions" value="599"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DG98"/>
    <dbReference type="GeneID" id="8295100"/>
    <dbReference type="KEGG" id="lth:KLTH0D03520g"/>
    <dbReference type="eggNOG" id="KOG0411">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020802_2_2_1"/>
    <dbReference type="InParanoid" id="C5DG98"/>
    <dbReference type="OMA" id="GLYVMQP"/>
    <dbReference type="OrthoDB" id="15270at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032216">
        <property type="term" value="F:glucosaminyl-phosphatidylinositol O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009447">
        <property type="entry name" value="PIGW/GWT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20661">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS W PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20661:SF0">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS W PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06423">
        <property type="entry name" value="GWT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017321">
        <property type="entry name" value="GWT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 8" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="74"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="112"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="151"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="191"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="224"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="254"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="294"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="353"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="385"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="427"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="453"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU280819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR22440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="481" mass="53553" checksum="866A93D684024748" modified="2009-07-28" version="1">MEDLKLRKEQFVSGLTGSSIQEINAVASVALCGYVCWSLLRSTVADGKVMLLADFFFNWCAILLSITTYADNPLALNLLLVVPPLLLWLCVRPTRSQRDISRNSLQKAEFKLMRHPFITAYRGSMMIVTALAILAVDFRIFPRRFAKVETWGTSLMDLGVGSFVFSGGLVSSRALLQNKLSGGRVSALKKIIHALRSSGTILLLGLLRLYFVKNLEYQEHVTEYGVHWNFFITLSLLPMAMIPIDAIAEYIPRVAIALVISTVYELFMVFNKDLLTFLISGPRETFVGANREGIFSFVGYCAIFLWGQSTGFYTLGNVATKNNLLKPSVQPIKNLNNIGTWEKLASKGPLCGLLTWSFITLSATQIVFAWHPFNVSRRFANLPYVLWVVSFNLGALSLFCLVDKVFENSAIGSQTSVSLEAMNSNGLFLFLLANVTTGLVNMIMPTIDIAPVEAFSILFLYASFLASVSIFMLKKRIFIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGH9</accession>
    <name>C5DGH9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0D05456p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0D05456g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552959.1">
        <property type="nucleotide sequence ID" value="XM_002552913.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGH9"/>
    <dbReference type="FunCoup" id="C5DGH9">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGH9"/>
    <dbReference type="GeneID" id="8295187"/>
    <dbReference type="KEGG" id="lth:KLTH0D05456g"/>
    <dbReference type="eggNOG" id="ENOG502QVAE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093588_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGH9"/>
    <dbReference type="OMA" id="YIIHCPT"/>
    <dbReference type="OrthoDB" id="6082470at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="entry name" value="Leucine Aminopeptidase, subunit E, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028071">
        <property type="entry name" value="Macro-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002589">
        <property type="entry name" value="Macro_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14519">
        <property type="entry name" value="Macro_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00506">
        <property type="entry name" value="A1pp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="Macro domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51154">
        <property type="entry name" value="MACRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Macro" evidence="1">
        <location>
            <begin position="13"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="260" mass="29044" checksum="90C3127254CB7973" modified="2009-07-28" version="1">MKVILLDKNRALVSAWKKTLDGFKHVSFHIGTLEDLPQKVLGTNAAVVSPGNSFGFLGGGFDLAIQSFFGGKQFEKFFRKRIGEFYKPVGETTIVDLDHWARNGIRYIVHIPTVVAPTRKVFDTEHPVETGYQLVFNAMWSALVRAPKNVETLIVPGLGTGYLGIPKAVCSKAMAFALNLFFANETMSVELRNVLIMHFLGFRYEGFFPADYKEECAKLGIELDKLKHFNVCKHPIAHLLPNEHLAAQEGDTSEPIHKPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGP2</accession>
    <name>C5DGP2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0D06908p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0D06908g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553022.1">
        <property type="nucleotide sequence ID" value="XM_002552976.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGP2"/>
    <dbReference type="STRING" id="559295.C5DGP2"/>
    <dbReference type="GeneID" id="8295253"/>
    <dbReference type="KEGG" id="lth:KLTH0D06908g"/>
    <dbReference type="eggNOG" id="KOG0381">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_593216_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGP2"/>
    <dbReference type="OMA" id="YRIARKE"/>
    <dbReference type="OrthoDB" id="5550281at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd22012">
        <property type="entry name" value="HMG-box_ABF2_IXR1-like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050342">
        <property type="entry name" value="HMGB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112">
        <property type="entry name" value="HIGH MOBILITY GROUP PROTEIN DSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="4">
        <location>
            <begin position="229"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="HMG box" evidence="4">
        <location>
            <begin position="302"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="2">
        <location>
            <begin position="229"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="2">
        <location>
            <begin position="302"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="99"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="362"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="16"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="30"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="44"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="104"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR22584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="381" mass="43557" checksum="BE40D442F1B79167" modified="2009-07-28" version="1">MNVPNVGTGISPPPKGSSNHGSIPNTNNSLQDQLLPYPDLDPTAHQQPSQQVSSKLQQLGPQIHHIPQQQPVHQGVQTNAGHPDIPVQADIYGQALQAHLQTPQQQHHQQQHQQQQQQQQQQQQQQFSDAMFNSFLTQIGQRQVANVLNTFNAGAHSMNAAMNSAMVTQPAQRFFHAAMNTPSMMEMTHPAMMQPTPQFQLQVPPPIAKKMSASQSRIEKRKMLKKQGPKRPSSAYFLFSMAVREDLVREYPDAKVPELSKLASAKWKEMSDEDKKPYHVKFKENWEKYRIARKEYESSLPPKRPSGPFIQFTQEVRPQIIRENPQKDLIEITKLIGEKWRSLSPVDKRTYTDTYKRKLKEWEEQYPEEASPTTPKDVLGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGR0</accession>
    <name>C5DGR0_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein uS10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">40S ribosomal protein S20</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0D07370g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553040.1">
        <property type="nucleotide sequence ID" value="XM_002552994.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGR0"/>
    <dbReference type="FunCoup" id="C5DGR0">
        <property type="interactions" value="898"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGR0"/>
    <dbReference type="GeneID" id="8295753"/>
    <dbReference type="KEGG" id="lth:KLTH0D07370g"/>
    <dbReference type="eggNOG" id="KOG0900">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_122625_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGR0"/>
    <dbReference type="OMA" id="IHKRVIH"/>
    <dbReference type="OrthoDB" id="10248551at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.600:FF:000004">
        <property type="entry name" value="30S ribosomal protein S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.600">
        <property type="entry name" value="Ribosomal protein S10 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00508">
        <property type="entry name" value="Ribosomal_uS10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001848">
        <property type="entry name" value="Ribosomal_uS10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027486">
        <property type="entry name" value="Ribosomal_uS10_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036838">
        <property type="entry name" value="Ribosomal_uS10_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005729">
        <property type="entry name" value="Ribosomal_uS10_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01046">
        <property type="entry name" value="uS10_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11700">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S10 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00338">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00971">
        <property type="entry name" value="RIBOSOMALS10"/>
    </dbReference>
    <dbReference type="SMART" id="SM01403">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54999">
        <property type="entry name" value="Ribosomal protein S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS10" evidence="6">
        <location>
            <begin position="21"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR22602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="118" mass="13438" checksum="2CCC53402FE773A1" modified="2009-07-28" version="1">MSDFQKEKGEQQEQPQIHKIRITLTSTKVKQLENVSANIIKNAENYKLVKKGPVRIPTKVLKITTRKAPNGEGSKTWDTYEMRIHKRYIDLQAPAQIVKRITQITIEPGVDVEVTVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGZ1</accession>
    <name>C5DGZ1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0D09438p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0D09438g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553121.1">
        <property type="nucleotide sequence ID" value="XM_002553075.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGZ1"/>
    <dbReference type="FunCoup" id="C5DGZ1">
        <property type="interactions" value="51"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGZ1"/>
    <dbReference type="GeneID" id="8295359"/>
    <dbReference type="KEGG" id="lth:KLTH0D09438g"/>
    <dbReference type="eggNOG" id="KOG0069">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019796_1_2_1"/>
    <dbReference type="InParanoid" id="C5DGZ1"/>
    <dbReference type="OMA" id="RGSCIDE"/>
    <dbReference type="OrthoDB" id="298012at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030267">
        <property type="term" value="F:glyoxylate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016618">
        <property type="term" value="F:hydroxypyruvate reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050223">
        <property type="entry name" value="D-isomer_2-hydroxyacid_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996:SF279">
        <property type="entry name" value="2-HYDROXYACID DEHYDROGENASE YPL113C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996">
        <property type="entry name" value="2-HYDROXYACID DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding" evidence="2">
        <location>
            <begin position="198"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR22683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="384" mass="42521" checksum="60B91E10B2C72F56" modified="2009-07-28" version="1">MAKKPGVLIPFKNRVEVDDSLPEWKRLEEHVDFIKYKITTKEDFKVALARSNVECLWITEDLFTYLGSPAEFYDDYSSSLKLIAVPWVGTDFLDGPKLKREKGIVVCNIGPSAASNVGELALFLALSCFRMTSFFEHCFRFVHRGQPGQCAEYVGGKKHALTASSATGGEHHYPFPEKFTREQAQVVDLSKNYTIAGKTIASPNGKTALILGFGYIGQSIGKKLCYGLDMKIQYFKRSGPVSKDILGYPAKYCSTLEDPETWVSADLIVLALPGHSSTQNVINAKTLSMCKDQVRIVNVGRGSCIDEDALFQALESGKVASAGLDVFKNESTKVDERFFDRWDVTLLPHIGSTVSDMVGRQTLVTLQNVEDVLVKNGRGLYPCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHB6</accession>
    <name>C5DHB6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0E02970p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0E02970g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS15 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553614.1">
        <property type="nucleotide sequence ID" value="XM_002553568.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHB6"/>
    <dbReference type="FunCoup" id="C5DHB6">
        <property type="interactions" value="289"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHB6"/>
    <dbReference type="GeneID" id="8291759"/>
    <dbReference type="KEGG" id="lth:KLTH0E02970g"/>
    <dbReference type="eggNOG" id="KOG2815">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073662_0_0_1"/>
    <dbReference type="InParanoid" id="C5DHB6"/>
    <dbReference type="OMA" id="FNMGRQY"/>
    <dbReference type="OrthoDB" id="441444at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00353">
        <property type="entry name" value="Ribosomal_S15p_S13e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.10">
        <property type="entry name" value="S15/NS1, RNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01343_B">
        <property type="entry name" value="Ribosomal_uS15_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000589">
        <property type="entry name" value="Ribosomal_uS15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005290">
        <property type="entry name" value="Ribosomal_uS15_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009068">
        <property type="entry name" value="uS15_NS1_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00952">
        <property type="entry name" value="S15_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23321">
        <property type="entry name" value="RIBOSOMAL PROTEIN S15, BACTERIAL AND ORGANELLAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23321:SF26">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US15M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00312">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01387">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47060">
        <property type="entry name" value="S15/NS1 RNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00362">
        <property type="entry name" value="RIBOSOMAL_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR23177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="291" mass="33656" checksum="EB4D13D1B03F3394" modified="2009-07-28" version="1">MKLPLAGLQRQWVIPQVAQVRMFASSRPCGGHKAVKFLKAQRKRQVNQAKQLKLKTALDTIDPVFGRSSTPFIVRVMAEMKEPKVLAQGYENQEVEKLLAAVEAAKREQIELSGFNNASLVPGASENDPEGLASKRESIMRILSMRNASNKDAMKLVVRQAREEFQRFEGDTGSSEVQAAIMTVRIQNLAKHIQENKNDHKNTRILRMLVQQRQNILRYLKRDKPERYFWTIQKLGLSDNAVVSEFNMDRRYMQDYKFFGDRILIKDSKKVADQKRRDLRKQKRSARRAQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHE9</accession>
    <name>C5DHE9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0E03806p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0E03806g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Endomembrane system</location>
            <topology evidence="6">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the YSP2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553647.1">
        <property type="nucleotide sequence ID" value="XM_002553601.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DHE9">
        <property type="interactions" value="62"/>
    </dbReference>
    <dbReference type="GeneID" id="8291796"/>
    <dbReference type="KEGG" id="lth:KLTH0E03806g"/>
    <dbReference type="eggNOG" id="KOG1032">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002908_0_0_1"/>
    <dbReference type="InParanoid" id="C5DHE9"/>
    <dbReference type="OMA" id="YIMVRQI"/>
    <dbReference type="OrthoDB" id="2162691at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140268">
        <property type="term" value="C:endoplasmic reticulum-plasma membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120015">
        <property type="term" value="F:sterol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032366">
        <property type="term" value="P:intracellular sterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13220">
        <property type="entry name" value="PH-GRAM_GRAMDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051482">
        <property type="entry name" value="Cholesterol_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004182">
        <property type="entry name" value="GRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031968">
        <property type="entry name" value="VASt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23319">
        <property type="entry name" value="GRAM DOMAIN CONTAINING 1B, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23319:SF4">
        <property type="entry name" value="GRAM DOMAIN CONTAINING 1B, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02893">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16016">
        <property type="entry name" value="VASt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00568">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51778">
        <property type="entry name" value="VAST"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1155"/>
            <end position="1175"/>
        </location>
    </feature>
    <feature type="domain" description="VASt" evidence="9">
        <location>
            <begin position="731"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="domain" description="VASt" evidence="9">
        <location>
            <begin position="934"/>
            <end position="1101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="190"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="232"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="345"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="623"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="658"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="899"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1103"/>
            <end position="1137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="44"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="70"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="112"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="232"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="369"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="396"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="456"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="489"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="626"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="669"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1103"/>
            <end position="1119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR23210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1291" mass="141852" checksum="16E3CEE65F43C5A7" modified="2009-07-28" version="1">MGLFSKKRKGSTKKGNKHEAPKRAPSTSAENNGRHTVARRSIRDSVTSNLTSDQGSSVAPGSATDTHFGSVKRRRSSPSPKRPKRRPSEIVKSKIGNLNHSKHLHPPDELRSPQSATESSHTSPKLSTKISVLSNTKPWNANTNTNSDTAEGGNPDNKSDYEARNSSGIFSTIVSACHNAANHLLTKTSDKSFSADSQPWMPKEKARTSDTQPQEPQNSAFLKHLDFLLSPSSMAQPNSQQTLLSSAPSQTGTNPSLASSRELRDSEISSQPSGHVDSIDGHASAAETEIMSLTDGIRFYPRKGESHIATFGKGNLTLDALSKTSSSDEPLDAGSFHERELHTLSVTNAAKNPTRQRGKSLHSFDAKTRVQNLEENGSTISPVKSDPGTKVSSPLNEKDDAADLSGKDVSKDVSKDASKLTEPNSEEKSRKRSSTFRIMCPRPSSPSLSALKVIPTRSQRNSMQRARSSSITESGLHSPSGLSSDEEEHSLVEGTDKKIHKPHISEKKNNEFHALFKDSGISPDEQLLADYSCAFSRDILLQGRIYISREHICFNSSILGWVTSFVIPFKEVVQIEKKSTAGIFPNGIVFQTLHSRYIFASFMSRDSAFDYITHVWNHVVVGTDGSGSPNGTSGSVGSNSRKRTMNSNFELETEGAGRLLSDGYSSGDETSDDEYEDETDMTTSDGEMSHTVRSASASSSQYDFETTNKSSVWTLGPSKHAPTSYDLDDADNRVVAETVFEAPLGKIVNILYGDDVSYTEQILKAQKNYNISNIPTILGTKERKYSYTKPLAGAIGPSKTSCEITESLEHYDLEDYVKAVQLSKTPDVPSGNLFVVKTSLYLSWAPRNSTKLTVVVSIEWSGKSWLKAAVEKGTYDGVTNTTKTLIEEVNKIIQITDKKSVKTPPSEGEAEVPNLPTAGPSEHASTSSHYTKEDGDVTIDDTIVIPAPLGTVYQLLFGSDTSYAQRIIEKQGNYDLTSVPKFKNDVREYQYMKPLSGPVGPKKTKCLIEEKIEHKDFEDYVVARQITKTPDVPSGSSFSVHTKFHIYWGPDNSTRILVVSKVVWTAKSWIKGAIEKGSLEGQKSSIKVLEQELKDIVSNAGKIKKTPAKRSPRPRGKRQRTSEKAPIVDSSASSKGNTSFTSILKQFFNVSLSSYQMLGVIFVLFLVFGATVRAFMGGNSNSFRVVKPGRIVIDGNSYNYAPTLNTLYGAYEQEVRSRKSVSRSPNIVTSSEGEIWEWIKDRGNILPEDHSKKSARKRLTIHKRQELEEAVRLAEDKLKELKIQLNDSRLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHF6</accession>
    <name>C5DHF6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein phosphatase</fullName>
            <ecNumber evidence="1">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0E03960g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PP2C family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="1"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553654.1">
        <property type="nucleotide sequence ID" value="XM_002553608.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHF6"/>
    <dbReference type="FunCoup" id="C5DHF6">
        <property type="interactions" value="708"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHF6"/>
    <dbReference type="GeneID" id="8291803"/>
    <dbReference type="KEGG" id="lth:KLTH0E03960g"/>
    <dbReference type="eggNOG" id="KOG1379">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029404_7_0_1"/>
    <dbReference type="InParanoid" id="C5DHF6"/>
    <dbReference type="OMA" id="ANTIAWM"/>
    <dbReference type="OrthoDB" id="60843at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000093">
        <property type="entry name" value="Type 2C protein Phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039123">
        <property type="entry name" value="PPTC7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12320">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12320:SF1">
        <property type="entry name" value="PROTEIN PHOSPHATASE PTC7 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07228">
        <property type="entry name" value="SpoIIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0464">Manganese</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="2">
        <location>
            <begin position="77"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU366020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR23217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="342" mass="37046" checksum="2BF98BBCAD2CFE73" modified="2009-07-28" version="1">MFASSRTAGMRGFSASRSMCLWAALAVLLAMVLTAWPGAYRFKRWQYRSFFSGPSGQGQSGGSAGFNYKYAVAYQAKDRSDSLYAGLDVDSPTGEDNYFLSARSTSDLYAGVADGVGGWVEHGHDSSAISRELCAAMSEFAMLTKDNRSFTPKQLIDMAYSKIKQEGQVKAGGTTAIVAHFPPSGKLELANLGDSWCGVFRDSKLVFQTKFQTVGFNAPYQLAIIPKDLAKEAAGRGSSYIQNKPSDADEYQFQLKSNDVIVLATDGVTDNIATGDMELFLGNNANATDLQQVTQKFVNQVVNLSKDSSFPSVFAQEISKLTGKQYLGGKEDDITVVVVRVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHI0</accession>
    <name>C5DHI0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0E04488p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0E04488g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553678.1">
        <property type="nucleotide sequence ID" value="XM_002553632.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHI0"/>
    <dbReference type="GeneID" id="8291827"/>
    <dbReference type="KEGG" id="lth:KLTH0E04488g"/>
    <dbReference type="HOGENOM" id="CLU_2292210_0_0_1"/>
    <dbReference type="InParanoid" id="C5DHI0"/>
    <dbReference type="OrthoDB" id="4056532at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018860">
        <property type="entry name" value="APC_suCDC26"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10471">
        <property type="entry name" value="ANAPC_CDC26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="50"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR23241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="101" mass="11360" checksum="061A109FBC7BC30B" modified="2009-07-28" version="1">MIRREPTVIGLSPEDLAELEEELEEFKLQREIKAQQRNLQRSATVLAEADLLESEAPVRDDTTHESPLQKPLGQQAPHRPPSQSVNAGPHAPRSNPFFSQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHK4</accession>
    <name>C5DHK4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mediator of RNA polymerase II transcription subunit 22</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Mediator complex subunit 22</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0E05016g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the Mediator complex subunit 22 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553702.1">
        <property type="nucleotide sequence ID" value="XM_002553656.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHK4"/>
    <dbReference type="FunCoup" id="C5DHK4">
        <property type="interactions" value="162"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHK4"/>
    <dbReference type="GeneID" id="8291851"/>
    <dbReference type="KEGG" id="lth:KLTH0E05016g"/>
    <dbReference type="eggNOG" id="ENOG502S77A">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130571_0_0_1"/>
    <dbReference type="InParanoid" id="C5DHK4"/>
    <dbReference type="OMA" id="WLLTQIP"/>
    <dbReference type="OrthoDB" id="203279at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.160">
        <property type="entry name" value="Mediator of RNA polymerase II transcription subunit 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009332">
        <property type="entry name" value="Med22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016530">
        <property type="entry name" value="Med22_Saccharomyce"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06179">
        <property type="entry name" value="Med22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007936">
        <property type="entry name" value="SRB6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="30"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR007936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR23265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="122" mass="13815" checksum="6DA739AEB7E47F16" modified="2009-07-28" version="1">MSNRGLLEQLERTTETLSHSLSNLIKYSSVAKERDDEDSGEQHELPESTAKNSTSGLMMVNAQTAQLIKGIQDLLVMTRSIREKWLLTQIPDEGVDSQSELDYEKCSQLLKQWTQEVVSSEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHR1</accession>
    <name>C5DHR1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">eIF-2-alpha kinase activator GCN1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0E06424g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GCN1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553759.1">
        <property type="nucleotide sequence ID" value="XM_002553713.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DHR1">
        <property type="interactions" value="1550"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHR1"/>
    <dbReference type="GeneID" id="8292400"/>
    <dbReference type="KEGG" id="lth:KLTH0E06424g"/>
    <dbReference type="eggNOG" id="KOG1242">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000504_1_0_1"/>
    <dbReference type="InParanoid" id="C5DHR1"/>
    <dbReference type="OMA" id="KYATQRG"/>
    <dbReference type="OrthoDB" id="5148094at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030295">
        <property type="term" value="F:protein kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904688">
        <property type="term" value="P:regulation of cytoplasmic translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000090">
        <property type="entry name" value="eIF-2-alpha kinase activator GCN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000096">
        <property type="entry name" value="eIF-2-alpha kinase activator gcn1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022716">
        <property type="entry name" value="Gcn1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056810">
        <property type="entry name" value="GNC1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057546">
        <property type="entry name" value="HEAT_GCN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056809">
        <property type="entry name" value="HEAT_GCN1_fung"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23346:SF7">
        <property type="entry name" value="STALLED RIBOSOME SENSOR GCN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23346">
        <property type="entry name" value="TRANSLATIONAL ACTIVATOR GCN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12074">
        <property type="entry name" value="Gcn1_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24993">
        <property type="entry name" value="GNC1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24984">
        <property type="entry name" value="HEAT_EF3_GNC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24987">
        <property type="entry name" value="HEAT_EF3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23271">
        <property type="entry name" value="HEAT_GCN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25801">
        <property type="entry name" value="HEAT_GCN1_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24916">
        <property type="entry name" value="HEAT_GCN1_fung"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="TOG" evidence="5">
        <location>
            <begin position="1320"/>
            <end position="1551"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="4">
        <location>
            <begin position="1492"/>
            <end position="1530"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="4">
        <location>
            <begin position="1604"/>
            <end position="1649"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="4">
        <location>
            <begin position="1649"/>
            <end position="1688"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00072275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR23322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="2673" mass="296506" checksum="ECBF789F80F0899E" modified="2009-07-28" version="1">MNLFKDSLEGVGPILEKSCRESSLNARVPVLEEVHLILQDQGQELGEPVIQEIANTLLESYVYIRDQKSGRLITSIFEDLFLRRPALLESSNTFIFNTLKKSLGTKAISDYLNLLDWTNAFLKFAACHSADFTVDSAKLLPTLCLVVYCIEIALDGHEDTKRSKLSQNQHRKRIRQSILQSLNKTLAFCLRHGKGVSVDTIVNGTLLSTVSKLPPAGLIIVMGSLVKAVLQLLPSQPAHHAALEVDFVVPFCEYVGKEALLGKEPPSPFCIETFFAPFAQEYVTSDLFKTYILPSLEKCILRSAEAGLTLANELYICIDSQKIDLCSIFTESKLMSQSFSSLKSSKDSVRKAALESMLTLLRMLRPETCNPESLRKFVNELFKNLKSNLNTDYKTVVSRLLLVTPPFFPEAVSDIVKGLTPYVSKESNEAALEPMLKAYFAHYSKMGSSTKEGYAVIKAGLNDKKQQVRRLWVVSLVEVIDASWEDAIHEFLPMILDFMRDILMSPFKHPARSILSCFVCVDRAHETEKNTLSTELHSLIDDIHQKSHSLGDAWLVATTSANLAAEDRKAALSLLSRAYERHPLMVGLDVIKSLEEETRQGFASGKNGVSIRYSTPVFNVLACTIDDKAALEGILVDLLVLAQHPGFKLKNGWAGLVMQAQLDPNHLVLKFADKIVEKFETYLGGEHIALSPLEKATVKSLSYAAFINSSATAPLLVGIFERDFDNNRLSHIDDSQIKIWRGAEGQLVVNVLDKKDKKLEDKNSKDYETLKWEEDIRKKQAKKNAPSRKLSKEEKELVDAQLSKESSVRKNVNKIVSELRRSCLLVQELVHGARLVDNGTDTWFSVAVSRLLSALRQEVFTELLPGDGVETFLDLSNVISERLGNIRYFTAVATLRSYGVHAIPENLTQEPLLDLISRVLFRIKFAANKKRFESLTLTFLLPLLSKVLEDGKNVSLKNASKPLVKSEFIEEDKEEEHLLLAMEIISSHAESFSDPAIPREHILEVLLSLLSVPSKARSSKECFLALCQNISISPNAEDLELILGTLMSPSEFVRATILEAIDDEFELSPYMKFSPQIFILMQDASESNRDTASFIWQFNKFEVNEELMISLLNFFAQTDSGLRLFVARAYANALRILTAKSEGFFEKYLGVLMDFYSSKARVPDPIVDEYGLVVMSSEAQKDHWEERSTAAIAFKEFSTFYPEDSECAVKFVEFLVNSGALGDRELLVRQELKEAGIEIIAHHGIRNVEKLIPVFERELAENNDVATKENVIILYGTLARHLPAEDSRVVVILERLLNTLNTPSEAVQQAVSACISPLVKLLGSQVEGYIEDTVQKLLNSRETMTSRRGAAWGLAGLVKGFGIGALSQFDIIRNLVDASEDKKDPVRRESVAFAFECLSKVLNKFFEPYVIEVLPNILKNLGDSVPEVREATANATKAIMANTTGFGVKKLIPVAVNNLDDISWRTKRGSVELLGNMAYLDPTQLSASLSNIVPEIVAVLNDSHKEVRKAADQSLNRFGEVIRNPEIQKLVPTLIKAISDPTKHTEEALDALIQTQFVHYIDGPSLALIIHVIHRGMRERSANTKRKSCKIVGNMAILVDSNDLVPYLQQLIDEVELAMVDPVPNTRATAARALGALVERLGEEQFPHLIPRLMSTLSDESRAGDRLGSAQALAEVISGLGLSKLDELLPNIMAGVTNYKTYVREGFMPLLLFLPVCFGAQFAPYISQIIQPILAGLADVDESISDTSLKAGKLIVKNYATKAIDLLLPELEKGMFDENERIRLSSVQLTSDLLFQVTGISSKNEFTDEEGDFNHEVTKQLVEVLGQDRRDRVLSALFVCRSDSSGVVRATTVDVWKAIVPNTSRTVKEILPTLTNIIVVHLASPSATLRHIAAQTLGDLVRRVGGNALSQLLPTLENSLIESNGSDSKQGVCIALCELTQSSSPENLVEYQAEIVNIIRSTLVDDDVVVREAAALSFDAFQEVFGKVAIDEVLPHLLNMLTSSEDSEYALLALQEIMSTKSEVIFPVLIPTLLQPPIDAFRARALGSLAQVAGSALYKRLSLIINALVNTLERNDVDDPTNIVLEDALDKVLSSVTDDEGMHPLLQQILSLMKDENKKKRVIVLQRLANFFKNTVLDMNIYIPEIISQSVVLFDDENPHVLQSVFDELTVLVKKQDKLMLEKLVKPAKQALQLVGRGDEELEVFKLPKGPSCILPIFLHGLMYGSNEDRELSAMAIADVVKRTPATNLKAFVTVITGPLIRVVGERFPSDVKAAILYALNVLFAKIPQFLRPFIPQLQRTFVKSLSDSSNETLRLRAAKALGSLIEYQPRVDPLVVELVAGARQATDSGVKTAMLKALLEVVSKAGSKLNENSKSSIINLVEEEILVVDNKLATAYAKLIGSLSEIMTKEEAANILNEKVLQVDLSSESGRFAILTLNSFLRDAPAHILQSEVVSELVNYLIVAANSNSPYISDNALTAIGKLLLLENEKKSPFSKRESETFFALGEQNIRALVEVLAKDMLVPASNSLDSRRLSLVVVRTLARFKFDECVKPYYDQLGPSVFSCLRDTIIPVKLAAEKAYLAIFRLVEEEDMCSFESWFKGLTGPSVKSSAGSEIQLRSIGDYTKRVGKRLASVERERIAAGGDPETVFSDRYEDESEIWAVGGVELVKDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHW4</accession>
    <name>C5DHW4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0E07656p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0E07656g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the importin beta family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553812.1">
        <property type="nucleotide sequence ID" value="XM_002553766.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHW4"/>
    <dbReference type="FunCoup" id="C5DHW4">
        <property type="interactions" value="253"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHW4"/>
    <dbReference type="GeneID" id="8291969"/>
    <dbReference type="KEGG" id="lth:KLTH0E07656g"/>
    <dbReference type="eggNOG" id="KOG2022">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_284018_0_0_1"/>
    <dbReference type="InParanoid" id="C5DHW4"/>
    <dbReference type="OMA" id="WMLQYMN"/>
    <dbReference type="OrthoDB" id="2016913at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051345">
        <property type="entry name" value="Importin_beta-like_NTR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12363:SF33">
        <property type="entry name" value="IMPORTIN-13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12363">
        <property type="entry name" value="TRANSPORTIN 3 AND IMPORTIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR23375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="968" mass="109241" checksum="3B7D2AF972A402B0" modified="2009-07-28" version="1">MLHLSRLTSIFVGDPAQNRSVLVVIKKLMSNAAQVFFRLNSGDGGSTPTTWKNPLETFVLLVSQPDVYSSASDDLFTHMLAQGVPYDDLIHFISSSDTLNILLLLFSEVIIEDLLKYQTSKLGASNLHNVVRETLYISSMSIINFNLQKLVAAFQSRTNTGPNVAENIFRCVNIWINYAIMVRTRSQGQIDLSETFENLIQLLCLYDSDQGFPYSETVVSILAEIFANDPTMLSFELRASIEEIFLGVRRGSGGTSGGHTWMLHFMNHLVVNQSFEELKELALCVVDFLQVSNLDVCNKLFTQSANTDSVNMDQYIKVLLQMTNFTLAPVLEEFFSVRMVDFWLDLCDGYNNLVRETLKPNTPIIASDLFGQVVQIYLPKVSLINKQRIIQEGEEESLLHEFDDFRGAVLDLMESMWSVLGNEKLTDILIGSVAQVSPKSAEDLFQIEAMCFCLNKLLSDMTLSESPGICATFGANENFLPNVLLLIQTSCQQKSPANGKEAQILSCDFVKTGSSILATVADYLKQNDKNLRDCMDVLFSSLETCSNADQVDTKFELLLTKCITSICETSRSELTSYLPTFTKIQHAMLQKSSKVSDFSKQGFTRCLGYIIQTYTSDGPESQAQYILQVIDTIKTEVTNSQEDRTQVLCLLNCLSELGSALIQPEEPEDPAYLAQLAQFQDFWQNDPLRLRVVILDLLESVLAQHGRDSEFVEAACLIMGKKLTLPDDEPHFLSFSMQELMEFLLRRCATCEPLTGLPYIIYLLEKVVNHHRASLTPQDFDFMLTKFFLGQHRDAVASDPDLTQNMISFVNSVMDTRPGLAVHCDHWATFVLPEFMRYLSSKERFTISAVTKFWTKVINNKKYSRHDEITIREQVLALGPQLTLQTTGALLSSQRSDLSYYSDLLRVLIARYPLQSKQWLSHTLPQLCENHRAHQLLVEKLFVTRGSRAAADAVLEWWLSCNGLPRLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>C5DID7</accession>
    <name>C5DID7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0E11704p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0E11704g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Ornithine decarboxylase (ODC) antizyme protein that negatively regulates ODC activity and intracellular polyamine biosynthesis in response to increased intracellular polyamine levels. Binds to ODC monomers, inhibiting the assembly of the functional ODC homodimer, and targets the monomers for ubiquitin-independent proteolytic destruction by the 26S proteasome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Interacts with ODC and thereby sterically blocks ODC homodimerization.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ODC antizyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553985.1">
        <property type="nucleotide sequence ID" value="XM_002553939.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DID7"/>
    <dbReference type="FunCoup" id="C5DID7">
        <property type="interactions" value="34"/>
    </dbReference>
    <dbReference type="GeneID" id="8292152"/>
    <dbReference type="KEGG" id="lth:KLTH0E11704g"/>
    <dbReference type="eggNOG" id="ENOG502RZPH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087076_0_0_1"/>
    <dbReference type="InParanoid" id="C5DID7"/>
    <dbReference type="OrthoDB" id="4033519at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008073">
        <property type="term" value="F:ornithine decarboxylase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0075523">
        <property type="term" value="P:viral translational frameshifting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002993">
        <property type="entry name" value="ODC_AZ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02100">
        <property type="entry name" value="ODC_AZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0688">Ribosomal frameshifting</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR23548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="196" mass="22325" checksum="4458BBB0CA58ED2E" modified="2009-07-28" version="1">MAARSGIRDTATTPRADKAALTELYWDAILLTESEFLDSGYHDYTGFQRQVVRQVGQALTTYERGHKAPFRSSSVALWRQIGLSYSLLYYPDWFQNPIWCKSDSTRLQVILSQDISQVVKQLLMSLLEYTATLDLAWLRLCVLRDVTGIKDLLRNLSWLGGRVVPNENRTAALDSLTALECATFSDEKYVIVEFEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5DII2</accession>
    <name>C5DII2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ATP synthase subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0E12716g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.</text>
    </comment>
    <comment type="function">
        <text evidence="9">Subunit b, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel. These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro. Part of the complex F(0) domain. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. In yeast, the dimeric form of ATP synthase consists of 17 polypeptides: alpha, beta, gamma, delta, epsilon, 4 (B), 5 (OSCP), 6 (A), 8, 9 (C), d, E (Tim11), f, g, h, i/j and k.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the eukaryotic ATPase B chain family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554030.1">
        <property type="nucleotide sequence ID" value="XM_002553984.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DII2"/>
    <dbReference type="FunCoup" id="C5DII2">
        <property type="interactions" value="504"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DII2"/>
    <dbReference type="GeneID" id="8292198"/>
    <dbReference type="KEGG" id="lth:KLTH0E12716g"/>
    <dbReference type="eggNOG" id="KOG3976">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077208_0_0_1"/>
    <dbReference type="InParanoid" id="C5DII2"/>
    <dbReference type="OMA" id="YTEWADG"/>
    <dbReference type="OrthoDB" id="67388at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.2210:FF:000002">
        <property type="entry name" value="ATP synthase subunit 4 mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.2210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008688">
        <property type="entry name" value="ATP_synth_Bsub_B/MI25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013837">
        <property type="entry name" value="ATP_synth_F0_suB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12733:SF3">
        <property type="entry name" value="ATP SYNTHASE F(0) COMPLEX SUBUNIT B1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12733">
        <property type="entry name" value="MITOCHONDRIAL ATP SYNTHASE B CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05405">
        <property type="entry name" value="Mt_ATP-synt_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161060">
        <property type="entry name" value="ATP synthase B chain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0138">CF(0)</keyword>
    <keyword evidence="4 9" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="6 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU368017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR23593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="236" mass="25704" checksum="FC6FB4A96C5F48C3" modified="2009-07-28" version="1">MSLRALTMKASTRPLVMLGMRQAPIGARYMSTPAPQDPKEKASSILSALPGNSALSKTGILATSAAAAVYAISNELYVINDETILLATFSGFVAIAVKFLAPAYKDYADARVKQVSDVLNASRTKHVDAVKQRIDSVSELQSVSDTTKVLFDVSKETVELEAKAFELKQKVDLASEAKSVLDSWVRYEASVRQLQQQQLAETVIKKVTSELSNSKFQDKVLQQSVADVEKLFASLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIY9</accession>
    <name>C5DIY9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0E16258p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0E16258g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554187.1">
        <property type="nucleotide sequence ID" value="XM_002554141.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIY9"/>
    <dbReference type="FunCoup" id="C5DIY9">
        <property type="interactions" value="120"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIY9"/>
    <dbReference type="GeneID" id="8292359"/>
    <dbReference type="KEGG" id="lth:KLTH0E16258g"/>
    <dbReference type="eggNOG" id="ENOG502RNIV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028751_0_0_1"/>
    <dbReference type="InParanoid" id="C5DIY9"/>
    <dbReference type="OMA" id="WTFDTWN"/>
    <dbReference type="OrthoDB" id="2527403at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018803">
        <property type="entry name" value="Ish1/Msc1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47372">
        <property type="entry name" value="DAUER UP-REGULATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47372:SF33">
        <property type="entry name" value="LATE EMBRYOGENESIS ABUNDANT (LEA) PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10281">
        <property type="entry name" value="Ish1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002947857" evidence="1">
        <location>
            <begin position="20"/>
            <end position="708"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="708" mass="79271" checksum="CA45CA917D1144E1" modified="2009-07-28" version="1">MKVTGVVLLASLAAADVASFYDKWSSSDLQQYLKDSKQALQETGEQNLDALKKQAQDVWNRQTQPTPWWKFWERKTKLADFTSNQEPISEWFFDSWSAKDLRKLLSKNKVKFDVEASRDELAQAAKKNFNSISSKLGASGLYPSESYFQNWDEYDLTSWLDEYKVPYDKAHAKKDELLSKVRENIYDASRYVDDERLNLLEQLDFANQQISSKAGKVKDEVFNSWSTKELGEWLRSHKVKLEDKASHDRKYLLNVAQKNKKLLKDDAQWYLETAQKKASPFFGKTEEAAASIWDQTIGKFKNWKDYLYHDDNVINDTFLIGVESWPKKRLRSFLDARGVSYSVFSTRASLLDLVKQHRNKPVNNLTESPAVADFFDGWSFENLKEWVKDKNDDITSSGVYKSASNKVSEIMGHAQKKGGEAADYAMDKGNNAAEHASKVAKEKGSDALEYAKDKGSDAADYAKVKGSDAAEYAKDRGSSAAEYVQEKGSEAATQASKVAKKKGAEASKLAKEKGKDAKEYAMEKGSDATDYAKSKGSDAAEYAKEKGADAAEYAKEKGADAAEYAKEKGADTAEYAKEKGADAADYAKDKSYSAAGYAKEKTSDAAAYAKEKGADAAAAAQAKGSELSDAVADKADEWYRLFSSWSMDDLKGYAKSFGIRTGPTTTREKLVHQVKENTQWFFGMKQDPMYKKVPKKVKQAMSNVVFRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJ16</accession>
    <name>C5DJ16_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">KLTH0E16984p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0E16984g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554214.1">
        <property type="nucleotide sequence ID" value="XM_002554168.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJ16"/>
    <dbReference type="FunCoup" id="C5DJ16">
        <property type="interactions" value="74"/>
    </dbReference>
    <dbReference type="GeneID" id="8292392"/>
    <dbReference type="KEGG" id="lth:KLTH0E16984g"/>
    <dbReference type="eggNOG" id="KOG0254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_11_5_1"/>
    <dbReference type="InParanoid" id="C5DJ16"/>
    <dbReference type="OMA" id="MTLIMEG"/>
    <dbReference type="OrthoDB" id="6612291at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000023">
        <property type="term" value="P:maltose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000254">
        <property type="entry name" value="MAL31p Maltose permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF5">
        <property type="entry name" value="ALPHA-GLUCOSIDES PERMEASE MPH2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0462">Maltose metabolism</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="102"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="152"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="182"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="282"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="369"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="403"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="430"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="463"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="497"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="12">
        <location>
            <begin position="105"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00026248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR23777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="539" mass="60285" checksum="812D8609CA4C77A5" modified="2009-07-28" version="1">MKGISSLVNRKNINSGSGSSEVDILPNDRGVNSIKMEDLKKTKSDVELYHMEHAPGATSPDDDVDNDNVVTEIMNATQDAQAADESERTMPLMKALKTYPKAAAWSILVSTTLIMEGYDTAILGAFYALPIFQHKFGSENHHTGEWEISVSWQIGLSLCYVAGEIVGLQITGYFVDFMGNRYTLIFALFTLAAFTFILYFCKSLGMIAVGEALCGMPWGCFQSLTVSYASEICPLALRYYLTTYSNLCWLYGQLFAAGIMKNSQNKYANSELGYKLPFALQWIWPLPMAIGIFLAPESPWWLIKKGKIDQARKSLERTLSGKSVEKDQLVMRELEKIKLTIDQEQKLKDSEGSYMDCIKDKVNRRRTRITCLTWVGQTTCGAALIGYSTYFYEKAGVSTETSFTFSIIQYCLGIAATFISWWLSKYVGRFDLYATGLAFQAVMFFIIGGLGCSDTKGAKMGSGALLMVVSFFYNLGIAPVVFCLVSELPSSRLRTKTIILARNSYNVIQIVTMVLILYQLNSEKWNWGAKSGFFWGHFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJ32</accession>
    <name>C5DJ32_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0F13090p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0F13090g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554758.1">
        <property type="nucleotide sequence ID" value="XM_002554712.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJ32"/>
    <dbReference type="STRING" id="559295.C5DJ32"/>
    <dbReference type="GeneID" id="8292982"/>
    <dbReference type="KEGG" id="lth:KLTH0F13090g"/>
    <dbReference type="eggNOG" id="KOG4209">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1787180_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJ32"/>
    <dbReference type="OrthoDB" id="4726at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF12">
        <property type="entry name" value="EUKARYOTIC INITIATION FACTOR 4B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="55"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR24321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="145" mass="16898" checksum="7314771C98D68BDE" modified="2009-07-28" version="1">MANREQDELLTYLNEWNMNTRKIQASTPHKVKSQSCKVVSKSENEISEQRKTDARSIFLRNIPVDISINLLEDHFKLYGTINRVTIFTNRKTFNLGYAYIEFEEDCSADQALDLNNSELCGHKIEVTKKRTNLPGIRRKPYKKNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJA5</accession>
    <name>C5DJA5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0F14850p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0F14850g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554831.1">
        <property type="nucleotide sequence ID" value="XM_002554785.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJA5"/>
    <dbReference type="FunCoup" id="C5DJA5">
        <property type="interactions" value="117"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJA5"/>
    <dbReference type="GeneID" id="8293060"/>
    <dbReference type="KEGG" id="lth:KLTH0F14850g"/>
    <dbReference type="eggNOG" id="ENOG502RZW2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098356_1_0_1"/>
    <dbReference type="InParanoid" id="C5DJA5"/>
    <dbReference type="OMA" id="AFMWFLW"/>
    <dbReference type="OrthoDB" id="5423111at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032126">
        <property type="term" value="C:eisosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070941">
        <property type="term" value="P:eisosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008253">
        <property type="entry name" value="Marvel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052649">
        <property type="entry name" value="NCE102-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28165">
        <property type="entry name" value="NON-CLASSICAL EXPORT PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28165:SF1">
        <property type="entry name" value="NON-CLASSICAL EXPORT PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01284">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="41"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="70"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="127"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="6">
        <location>
            <begin position="6"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR24394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="171" mass="18308" checksum="102042DBA08D318F" modified="2009-07-28" version="1">MLSILDNSLRALNFAFLVIILGLTGSLAATHKTHNNPQVNFAIFTAAFGLLFDSLYAIPANFVSALAWPLLIAVFDFLNFVFTFAAATALAVAIRTHSCTNQDYLDSNTVTQGSTDRCRKAQASVAFLYFSFFIFLAKLGLSLVNLISVGAFGAGSSRRTGNVGVPTISQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJB2</accession>
    <name>C5DJB2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0F15004p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0F15004g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554838.1">
        <property type="nucleotide sequence ID" value="XM_002554792.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJB2"/>
    <dbReference type="FunCoup" id="C5DJB2">
        <property type="interactions" value="170"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJB2"/>
    <dbReference type="GeneID" id="8293067"/>
    <dbReference type="KEGG" id="lth:KLTH0F15004g"/>
    <dbReference type="eggNOG" id="ENOG502S0Z0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028632_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJB2"/>
    <dbReference type="OMA" id="PLEFYPQ"/>
    <dbReference type="OrthoDB" id="4489171at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14307">
        <property type="entry name" value="UBA_RUP1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041970">
        <property type="entry name" value="Rup1_UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055335">
        <property type="entry name" value="Ucp6/RUP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39597">
        <property type="entry name" value="UBA DOMAIN-CONTAINING PROTEIN RUP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39597:SF1">
        <property type="entry name" value="UBA DOMAIN-CONTAINING PROTEIN RUP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBA" evidence="2">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR24401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="597" mass="67192" checksum="8048EE05D166AC55" modified="2009-07-28" version="1">MSENAIQSLLDMGITREVAIEALAKTNGDVENSVNYIFSGELPQQGPPPAPEETPEDREKEPESESELPENPEFIEEAPPSEDEATDQQGVAVSPREQIACQLKNRPEDPLVVRPASGNALFENYFALFCLAVGLGFPHHFLEPDFQDLTYGQDWYKGQFLKPAYRVKFENNDDVSIVPQETLTSQDNLILQPELLWQFQKFLAVQNSPQCRRKYVTARYLTKVLEPQVVEKLNNSDHLHEALPSFIKSLANDVELCPGAHSVKELFISTAYYKPVSEPEPIETLVSLLHFMPEEYDTNIYKMFNALLYPDSNEDAEMDDIQNSLGNLAPMITIVFDEMDESTEQATLPHGVDVPLEFYPQLYTEEAKNLLIKEVTKLSADSQTQARSILRNLNDLKSFQGKQIHSFLNSALDFITKDMSGVSEQSGVQELVDSLQEVKQQLGSKKTEKMAQYKAVSHRLNNELNLSHPELGIIQRAKDLGLIDEPYLLTSAVISASNYFIRQNNGQWFHVVGRSNSEHMDVTKTDASTVSSAIRFHTRCASESPLMFTYFKPSAIDTEAQVKEAVGHNKGAKLFAEQDQKHINTLSAPDEEDLIDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJQ0</accession>
    <name>C5DJQ0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0F18194p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0F18194g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the proteasome subunit S3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554976.1">
        <property type="nucleotide sequence ID" value="XM_002554930.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJQ0"/>
    <dbReference type="FunCoup" id="C5DJQ0">
        <property type="interactions" value="1256"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJQ0"/>
    <dbReference type="GeneID" id="8293211"/>
    <dbReference type="KEGG" id="lth:KLTH0F18194g"/>
    <dbReference type="eggNOG" id="KOG2581">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019858_1_2_1"/>
    <dbReference type="InParanoid" id="C5DJQ0"/>
    <dbReference type="OMA" id="AKLWFYI"/>
    <dbReference type="OrthoDB" id="1713558at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042176">
        <property type="term" value="P:regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050756">
        <property type="entry name" value="CSN3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013586">
        <property type="entry name" value="PSMD3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057985">
        <property type="entry name" value="TPR_PSMD3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10758:SF2">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10758">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 3/COP9 SIGNALOSOME COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08375">
        <property type="entry name" value="Rpn3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25573">
        <property type="entry name" value="TPR_PSMD3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00753">
        <property type="entry name" value="PAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0647">Proteasome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="4">
        <location>
            <begin position="253"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="465"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="465"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR24539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="499" mass="57153" checksum="644B2F55F94DCCBB" modified="2009-07-28" version="1">MTSDLMEVDSAPQTEVKTDSESTVDKVLHLLQDVSKTTTTLDSRFVWKSLRELSTLRSSLEKEDLAVLVNLLYPESSTFKVPLLKFINNNQKSKVDNAEEQRQQYPAAFYQLTGGNSVDVSGEVNSFLHLLVQLYLLDSNQVDHLKVFNLQVVIPKVLNFYNNRTLDLINAKLWFYICRAHELRGDAFDTSIRSEMVKFLKTATLKHDNETRAMLITFILRSFLQTGEVDAAADFIGKVEFPGSNDVSSPLEARYYFYLSKLSAIQLDYSTANEYVIAAIRKAPNTKKCIGFLQQANKLHCAIELLMGDIPELSFFKQKNMERSLLPYYHLSKAVKLGDLNKFTSAISTYKKELVHDGNYQLCVRLRSSVIKTGIRIISLTYKKISLKDICLKLRLDSEQTVEYMVSRAIRDGVIEAKINHEEGYIESSELLNVYATQQPQQLFDERIRFVNQLHDECVKAMRYPEDGNKDKNKSADHDQLDEMIDISDLDEDDLGDFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJY9</accession>
    <name>C5DJY9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">L-serine ammonia-lyase</fullName>
            <ecNumber evidence="5">4.3.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0F00440g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-serine = pyruvate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:19169"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="EC" id="4.3.1.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the serine/threonine dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.17" evidence="5"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554227.1">
        <property type="nucleotide sequence ID" value="XM_002554181.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJY9"/>
    <dbReference type="FunCoup" id="C5DJY9">
        <property type="interactions" value="557"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJY9"/>
    <dbReference type="GeneID" id="8292418"/>
    <dbReference type="KEGG" id="lth:KLTH0F00440g"/>
    <dbReference type="eggNOG" id="KOG1250">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021152_3_1_1"/>
    <dbReference type="InParanoid" id="C5DJY9"/>
    <dbReference type="OMA" id="AEQGCEH"/>
    <dbReference type="OrthoDB" id="7773036at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003941">
        <property type="term" value="F:L-serine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004794">
        <property type="term" value="F:threonine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006565">
        <property type="term" value="P:L-serine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006567">
        <property type="term" value="P:L-threonine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06448">
        <property type="entry name" value="L-Ser-dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000040">
        <property type="entry name" value="L-serine dehydratase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050147">
        <property type="entry name" value="Ser/Thr_Dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000634">
        <property type="entry name" value="Ser/Thr_deHydtase_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078:SF2">
        <property type="entry name" value="CATABOLIC L-SERINE_THREONINE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078">
        <property type="entry name" value="THREONINE DEHYDRATASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00165">
        <property type="entry name" value="DEHYDRATASE_SER_THR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="9" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="11">
        <location>
            <begin position="19"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR23790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="341" mass="37242" checksum="1FCCF11984CBC237" modified="2009-07-28" version="1">MKSAFVKTPLLAHYFAASSNRLPNQPQFMLKCEFLQPSGSFKSRGIGYMITKQAIKIRSEGNKLPHVFASSGGNAGLAAATTSRALSIPCSVVVPESTSNRMVQKIWNLGAEVHVYGRNWKEADKFLKESIISGHNPSRTQVIYAHPFDHPLIWEGHSSIVDEIIDSLKEQKIDLKNIKAIVCSVGGGGLYNGIIEGLSRHKLVHKIPIIGVESKGCEVFNSSLKLGKLTEFEDITSIATSIGTNSISKKTLENALRYHTKSVVLTDQEIISTCLRFADETNIIPEPACGAALHLGYYPDILYQALNSDFTPEDVIIMIGCGGSATSYNDLASLFSKKILQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKH0</accession>
    <name>C5DKH0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0F04598p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0F04598g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the U5 snRNP complex required for pre-mRNA splicing. Binds GTP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554408.1">
        <property type="nucleotide sequence ID" value="XM_002554362.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKH0"/>
    <dbReference type="FunCoup" id="C5DKH0">
        <property type="interactions" value="309"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKH0"/>
    <dbReference type="GeneID" id="8292603"/>
    <dbReference type="KEGG" id="lth:KLTH0F04598g"/>
    <dbReference type="eggNOG" id="KOG0468">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002794_11_2_1"/>
    <dbReference type="InParanoid" id="C5DKH0"/>
    <dbReference type="OMA" id="YIFRPIR"/>
    <dbReference type="OrthoDB" id="364892at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071007">
        <property type="term" value="C:U2-type catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030623">
        <property type="term" value="F:U5 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01683">
        <property type="entry name" value="EF2_IV_snRNP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.870:FF:000002">
        <property type="entry name" value="Translation elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000646">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.240:FF:000022">
        <property type="entry name" value="U5 snRNP-specific protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.870">
        <property type="entry name" value="Elongation Factor G (Translational Gtpase), domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005517">
        <property type="entry name" value="Transl_elong_EFG/EF2_IV"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908:SF6">
        <property type="entry name" value="116 KDA U5 SMALL NUCLEAR RIBONUCLEOPROTEIN COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908">
        <property type="entry name" value="TRANSLATION ELONGATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03764">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00889">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="9">
        <location>
            <begin position="116"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="16"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00055641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR23971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="938" mass="104875" checksum="A72477F497058B23" modified="2009-07-28" version="1">MDEELYDEFGNLIGGEAEDSDTQDESLVQEEESSALQIAEANPSTEIVHRETLATAFPDDVEILVETEDHQATTTPLVEPAEINVKKQALYTESIKHLPRVNYDRDYFSTLHEIPERTMNVTILGPLHSGKTSLVDLLILQAHKKLPGTSKNVREGWKPLKYMDNTKLEIERGISYKLNGFSFLATDLQQKSVALTVLDCPGHVNFIDEVAVSLAASDTCVIVVDVVEGVTSIVQQLIKQCEKHGLPIVFVLNKVDRLILELKLPPQDAYLKLANIVNQINSFSNGSYSPEQGNVLFSSAKLGFTFSIEEFVLYYYSKQLGMDKLEGFLKRMWGNIYFQDGRFSKVTNQSGLTTFSEFILIPLYKIFTHSLSNDPENLAVILKREFRVTLPREYFKIDPQPLLRKVLGLIFRDQAGFVGSVTSFGSFACEAAPLKKKFFDVAANENDTLAHVLKLMDYCGDVWALVRVYQGVLNKHAMIKVLDGGLPPEDDAPEVEIQDIALMGGRYVFPVEKAHEGQIVLVKGIDQYITKSGTLASSTSTIFPPIDYINDPVFKVVLQVLHPQELPLMLEGLKKVNKFYPGMFTCVEESGENVILGTGELYLDCLLYDLRTNYARIEIQVSSPLVKFSETCVGESFASIPVSGASGHLELSIAAQPLERELAEDLASGALAESEIQNKRQLSKKLREKYSWDSLAARNVWSLRSSNVLVDDTLPDETDKERLAEAKHSICQGFEWATREGPLAEEPIRGVHFRLLSVSMGDDVRAGQLIPLVRKACYVAMLTATPALLEPIYEACVLVHDDAADVVEELFSKRRGARVYNRVKIPATPLIELRGQIPVIDSIGFETDLRLATNGTGMCQLQFWNKIWRKVPGDVMDEDAVIPKLKPAPRDSMSRDFVMKTRRRKGLSSEGYATQDGPSLARYIEPELFQKLKDNNLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKJ8</accession>
    <name>C5DKJ8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0F05280p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0F05280g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554436.1">
        <property type="nucleotide sequence ID" value="XM_002554390.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKJ8"/>
    <dbReference type="FunCoup" id="C5DKJ8">
        <property type="interactions" value="105"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKJ8"/>
    <dbReference type="GeneID" id="8292634"/>
    <dbReference type="KEGG" id="lth:KLTH0F05280g"/>
    <dbReference type="eggNOG" id="KOG2425">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043823_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKJ8"/>
    <dbReference type="OMA" id="HWGTHER"/>
    <dbReference type="OrthoDB" id="10263222at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090730">
        <property type="term" value="C:Las1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000460">
        <property type="term" value="P:maturation of 5.8S rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000470">
        <property type="term" value="P:maturation of LSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007174">
        <property type="entry name" value="Las1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15002">
        <property type="entry name" value="RIBOSOMAL BIOGENESIS PROTEIN LAS1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15002:SF0">
        <property type="entry name" value="RIBOSOMAL BIOGENESIS PROTEIN LAS1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04031">
        <property type="entry name" value="Las1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="217"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="409"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="359"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="217"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR23999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="471" mass="54085" checksum="12039C5D0A97F28C" modified="2009-07-28" version="1">MVAHRLSHRAHQLMAQHEPRIVPWRYKSDLDGLKRAFYPKKFGTADRRREAVVKVRCWNTRGPYVPHTVESTAHLAETKMLDEQALLSGIPLQLSYTMAMIRFVNGLLDPTQQSQFAIPLHTLAERMGLPSWFVELRHCGTHERELPSLDMLRLASESALEWLWEHYWDADEEPAQEEEEQEQTPDVSAETVERVLRKASSIANLLTANGNMWKTQRVSSSFTGEQDPQQKRQKRAEKPEERLERFVLAAKDAWRSVKARPDVFVGALMRNYRSRQGPVLELLADRIQPFGFELCRWVCENYDAAIKQGHSPLRARFDAARLRALLGQVCDTTLDLKKVLAHWDRWAEMLRAHPNYVALKLLEGCEQQLQNAADKLRKRHAREVDELRALAQRLRACVVSSEFNMYQLAQPQPQDASPAESAPTVPQAQPVPSANDILNDLAQLKKTAQHRRAIRAWDPAPDWTPRPFGTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKK1</accession>
    <name>C5DKK1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0F05390p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0F05390g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554439.1">
        <property type="nucleotide sequence ID" value="XM_002554393.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKK1"/>
    <dbReference type="FunCoup" id="C5DKK1">
        <property type="interactions" value="32"/>
    </dbReference>
    <dbReference type="GeneID" id="8292637"/>
    <dbReference type="KEGG" id="lth:KLTH0F05390g"/>
    <dbReference type="eggNOG" id="ENOG502RYWP">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061864_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKK1"/>
    <dbReference type="OMA" id="KRINTHY"/>
    <dbReference type="OrthoDB" id="4051837at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR24002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="365" mass="40962" checksum="CFE6588FCD620DF8" modified="2009-07-28" version="1">MKRTFATTLSHLTKQKPADLRSTLKFLTQAGKGTTLTSLFENGKTEASHIKHIVDVLNNDLPDVELNKKRVDAHYDILMSRLKHVVEKSISGDDLSGARTAPGTSSQLSFQEKFARCHTSESLYSLLLELQVAHKLSRVAMAKIVMNRNFTHLKQVSENLPGFSHEVELAVMVGYRLRDADLNEIYKTYSSRWTDEWHNLTRIAQNLVWKSDHRLGGIAAVTRRMHEIKDWSSADIISLYQALYSVAHQLPVASSAELTKVQQLFVAALRALSRAVPTSEQAKKNCFAVVRASLENKLSAESSNSSEAGASVFQYRFMRRLDDLLQLSARDARLGPDVGTAIQGVLDSLHREEEQARSQMVLKFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKR8</accession>
    <name>C5DKR8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GTP-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0F06952g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">GTPase involved in activation of the TORC1 signaling pathway, which promotes growth and represses autophagy in nutrient-rich conditions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the GSE complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the GTR/RAG GTP-binding protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554506.1">
        <property type="nucleotide sequence ID" value="XM_002554460.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKR8"/>
    <dbReference type="FunCoup" id="C5DKR8">
        <property type="interactions" value="296"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKR8"/>
    <dbReference type="GeneID" id="8292708"/>
    <dbReference type="KEGG" id="lth:KLTH0F06952g"/>
    <dbReference type="eggNOG" id="KOG3886">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044099_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKR8"/>
    <dbReference type="OMA" id="QQKDHIF"/>
    <dbReference type="OrthoDB" id="10020193at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990131">
        <property type="term" value="C:Gtr1-Gtr2 GTPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010507">
        <property type="term" value="P:negative regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11384">
        <property type="entry name" value="RagA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.190:FF:000006">
        <property type="entry name" value="GTR1p Cytoplasmic GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000488">
        <property type="entry name" value="Small monomeric GTPase (Gtr1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006762">
        <property type="entry name" value="Gtr1_RagA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039397">
        <property type="entry name" value="RagA/B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11259">
        <property type="entry name" value="RAS-RELATED GTP BINDING RAG/GTR YEAST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11259:SF1">
        <property type="entry name" value="RAS-RELATED GTP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04670">
        <property type="entry name" value="Gtr1_RagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR24069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="299" mass="34435" checksum="55E307749C0AE7F3" modified="2009-07-28" version="1">MSSNNRKKLLLMGRSGSGKSSMRSIIFSNYSAFDTRRLGATIDVEHSHLRFLGNMTLNLWDCGGQDVFMENYFSQQKDHIFQMVQVLIHVFDVESQEVIKDIEIFTKALKQLKKYSPDAKIFVLLHKMDLVQRDKRQELFQIMMSKLQETSSAYGFPNLVGFPTSIWDESLYKAWSQIICSLIPNMATYQSNLKKLKSVMGAREIILFEKTTFLVICSSNTVSGELLDPKRFEKISNIMKNFKQSSMKLKSSFKTLVLNNTIYVSELSSNMMCFIVLNHSDDPQDLILENIKKAKAHFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKU9</accession>
    <name>C5DKU9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0F07700p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0F07700g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM14 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554537.1">
        <property type="nucleotide sequence ID" value="XM_002554491.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKU9"/>
    <dbReference type="FunCoup" id="C5DKU9">
        <property type="interactions" value="72"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKU9"/>
    <dbReference type="GeneID" id="8292742"/>
    <dbReference type="KEGG" id="lth:KLTH0F07700g"/>
    <dbReference type="eggNOG" id="KOG4267">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096652_3_1_1"/>
    <dbReference type="InParanoid" id="C5DKU9"/>
    <dbReference type="OMA" id="AYPAYIM"/>
    <dbReference type="OrthoDB" id="5620at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1740">
        <property type="entry name" value="Transmembrane protein 14-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005349">
        <property type="entry name" value="TMEM14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044890">
        <property type="entry name" value="TMEM14_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12668:SF53">
        <property type="entry name" value="TMEM14 PROTEIN HOMOLOG YJR085C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12668">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 14, 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03647">
        <property type="entry name" value="Tmemb_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="29"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="80"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR24100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="105" mass="11182" checksum="478E5DC49BC07578" modified="2009-07-28" version="1">MEHPAFTLAALTTMGGAMGYARKGSKPSLLAGLVFGGVYGYAGYLLHKNADGGLEIALGASSLMLITGIARGVPSRFKKPVPLVLTILGGLGTFYYYRKYKEFYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DL22</accession>
    <name>C5DL22_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0F09350p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0F09350g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554610.1">
        <property type="nucleotide sequence ID" value="XM_002554564.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DL22">
        <property type="interactions" value="184"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DL22"/>
    <dbReference type="GeneID" id="8292817"/>
    <dbReference type="KEGG" id="lth:KLTH0F09350g"/>
    <dbReference type="eggNOG" id="KOG0839">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005519_0_0_1"/>
    <dbReference type="InParanoid" id="C5DL22"/>
    <dbReference type="OMA" id="VTADRWM"/>
    <dbReference type="OrthoDB" id="241340at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016423">
        <property type="term" value="F:tRNA (guanine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18091">
        <property type="entry name" value="SpoU-like_TRM3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1280.10:FF:000022">
        <property type="entry name" value="Trm3p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045330">
        <property type="entry name" value="TRM3/TARBP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044748">
        <property type="entry name" value="Trm3/TARBP1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12029:SF11">
        <property type="entry name" value="METHYLTRANSFERASE TARBP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12029">
        <property type="entry name" value="RNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="tRNA/rRNA methyltransferase SpoU type" evidence="3">
        <location>
            <begin position="1246"/>
            <end position="1388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR24173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1396" mass="159489" checksum="1A974BB8E22C53F1" modified="2009-07-28" version="1">MSTNKLIGKYFPIEKQTELLQTLSTNEEFGEMFEVLGTMEFGKEALEVATQCTTEQVLKGFKGHNETEEWDVSKLRELAKLTSKLPNTFAALQEWIVGILDQNLQAPNDTNVKMLLEFLEQTHMFSEGQELPKSAKLDKQLVKLMQADDPQLAEISSKILKWKILDLSQKCMEDEEFDLFVWDELRKVFTCNTDTLKLKNGYLFILRFLMEDKISQRLHDFIVSDLFWESLKRGLEHDVHDLRKFSLSILKLTLQKLSPKDSFTNNWIQWKPELYAENIAAWKRFTTLYEIVALDTALNQFEAASADILDLFENKLLNPSWVLVIFSTGLKASMESVRRHTVRLMLRIKDKSVFQTSLNELSTVFLPAAMYAPYFSVIEKKCPYGELVSQFVAELVLHICEMEPKSGPFEMILAILSCLYQQRTSFEPAKIYIILGLYGALRQAKARSIGGSHIELIAKSFESESEDDVFEITLQTTYLKLLMHIEVDISPYTWIDALSRHVKAKGGSYRYIAPLIDDLIDVSVQCFDKNQIQDIFSDYDHEDSTFKTIAYLIFGTVPSAIEPSFLKELSKYGEVQTQFNENFSLLLSELISAKPPNSTYQGSHELSKLPVFGPSAWCTVDLHPLYQSLLNDFDAAKFKFFVCSYQQTVEHTMSTLDLNLDDLLKLYAFLKRHCQENVSKGFKYKDSIYGDFFMFLLSFLKCYALELNTSSKSNELDSILESLSSNVLRDNGNYQGNLAIIKVLHYLLSNYVTPRIQDFECEPQDDEAIVFQVVQILCSIWDSTSDERLVLNQKDLHLSFIECIFHPSVFFFALTYKDLELALAGTLSKYGKSIIQHSYSRRTLLPVLSQQINAFMEVYGKNLEWKQHNNDWLIVLMFEVFTQHQVNVNGFRLKPIIAKAFDEQIERLGGHSMGLYERVYKLPEVSARVFIISALLNANDEFKEKALDYMLNNDENLLKAKKSTDGPEELGRLLKWQLLLLTVKSVDHVTLSKYVEEKVLPSIITEASPLVRAYMEWISAIDLTQTYSEAHPLNNEDALFDMLKDHSRPLISITAERILFIALKGLSAGTFCDRLLQRFIAELILNCTSNKPLIRHFSNSLMLSFWPAFENLLKEGTLRNVLKNLYDNAQKTQVHGQFRLGDAIIWDADTDFTLTNIFGGVILKSTDHDVPYIESATFRRFLTCVDNVPVGQDQKHLWLKKRTRPKQEAEVSLQKDSPLQTKSGAWETVMDIDNQSSAVTVKRSPLIVIASLVDKAPNLGGICRLCDVLGAGLLTVHDIRIKNHPQFKSVAVTADRWMPMEAVTTQDITKFMRSKKQEGYTLIGLEQTDKSIQLDNKFQFPPKSLILLGTESEGIPGHLLSELDLCLEIKQSGVIRSMNIQTATAVIVHAYSAQHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLH3</accession>
    <name>C5DLH3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0G00726p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0G00726g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555071.1">
        <property type="nucleotide sequence ID" value="XM_002555025.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLH3"/>
    <dbReference type="FunCoup" id="C5DLH3">
        <property type="interactions" value="382"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLH3"/>
    <dbReference type="GeneID" id="8293329"/>
    <dbReference type="KEGG" id="lth:KLTH0G00726g"/>
    <dbReference type="eggNOG" id="KOG2402">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036626_1_0_1"/>
    <dbReference type="InParanoid" id="C5DLH3"/>
    <dbReference type="OMA" id="ISGCYAR"/>
    <dbReference type="OrthoDB" id="166375at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016593">
        <property type="term" value="C:Cdc73/Paf1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990269">
        <property type="term" value="F:RNA polymerase II C-terminal domain phosphoserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.200:FF:000005">
        <property type="entry name" value="Related to Pol II transcription elongation factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.200">
        <property type="entry name" value="Plus-3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004343">
        <property type="entry name" value="Plus-3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036128">
        <property type="entry name" value="Plus3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13115">
        <property type="entry name" value="RNA POLYMERASE-ASSOCIATED PROTEIN RTF1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13115:SF8">
        <property type="entry name" value="RNA POLYMERASE-ASSOCIATED PROTEIN RTF1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03126">
        <property type="entry name" value="Plus-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00719">
        <property type="entry name" value="Plus3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159042">
        <property type="entry name" value="Plus3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51360">
        <property type="entry name" value="PLUS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Plus3" evidence="7">
        <location>
            <begin position="242"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="441"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="501"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="73"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="90"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="123"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="163"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="188"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="226"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR24634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="561" mass="65770" checksum="58B539E995D4A4E6" modified="2009-07-28" version="1">MSDIDEDLLALAGASSGEEEEEDVQIGSSKKRSRSDHQSSSKRRIVDEDEEQDDDEEEGEEDDYNPEAASYDMDSEEDEDEEPNPFPLEGKYKNEEDRSYLESLPEMERETMLFERSQTMQKYQERRVLKERAKNIRDQQQKRQLQQEGKKTRSSTRSTRTTGHSDLKESRLSELKKQRAKKKGTYEYSEEEEEEGQNEEDEYGYQDQEDESEDDYEPMYKKGKTEDEEELKWAEDEDLDREPELGDFNRIKIGRSFVAKFCFYPEFNDMIKGCYGRVNVGVDKRSGQTLYRMVKIEKVFLQKPYNMGKFFTNQYFGVTQGKNRKVFQMNFFSDGAITQPEFERYLRSLETSEINKPSLYIINNKSNEINSFVSQPVTSKLTDELVRNRMIFNKKLSGTNAVLEKTILKDKLQYAKESGSERDVAKYSSQLRNLEKRLSSYEKHHENDQAGSKKLGALTSKNRRVNLDKGKNVEVTKKEDTPFDAKTDPFSRLKTRTKIYYQEIQREENEKAKELARQEQLQKDDLNLVEKERKLLLAKFKNLGGLEDIISNMDFQVSLDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLK5</accession>
    <name>C5DLK5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0G01430p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0G01430g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555103.1">
        <property type="nucleotide sequence ID" value="XM_002555057.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLK5"/>
    <dbReference type="GeneID" id="8293361"/>
    <dbReference type="KEGG" id="lth:KLTH0G01430g"/>
    <dbReference type="HOGENOM" id="CLU_1603018_0_0_1"/>
    <dbReference type="InParanoid" id="C5DLK5"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="30"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="166" mass="17903" checksum="3CEB3289D46B0A79" modified="2009-07-28" version="1">MPPSASPPAAPSAARLNGTPDTPTLPARKPPTSASPSFRAPASAAPRGARRAPCKREPFASALSCGAAPCSRDLPRCWAYSRGSSSDVATVSALYPRRRPVAKDLRPDKRIKCKNMRKAPPSSRHCSSLWSLCATFPRPLRSQRGILQFSRPRKNVVRRVPRAHGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLN8</accession>
    <name>C5DLN8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0G02244p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0G02244g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555136.1">
        <property type="nucleotide sequence ID" value="XM_002555090.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLN8"/>
    <dbReference type="FunCoup" id="C5DLN8">
        <property type="interactions" value="172"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLN8"/>
    <dbReference type="GeneID" id="8293398"/>
    <dbReference type="KEGG" id="lth:KLTH0G02244g"/>
    <dbReference type="eggNOG" id="ENOG502QVZD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_320281_0_0_1"/>
    <dbReference type="InParanoid" id="C5DLN8"/>
    <dbReference type="OMA" id="LEWFTPN"/>
    <dbReference type="OrthoDB" id="4032152at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11602">
        <property type="entry name" value="Ndc10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.20">
        <property type="entry name" value="Centromere DNA-binding protein complex CBF3 subunit, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038279">
        <property type="entry name" value="Ndc10_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031872">
        <property type="entry name" value="NDC10_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049055">
        <property type="entry name" value="NDC10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022210">
        <property type="entry name" value="TF_GCR1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12550">
        <property type="entry name" value="GCR1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16787">
        <property type="entry name" value="NDC10_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21400">
        <property type="entry name" value="Ndc10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NDC10 N-terminal" evidence="5">
        <location>
            <begin position="14"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Ndc10" evidence="4">
        <location>
            <begin position="139"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription activator GCR1-like" evidence="3">
        <location>
            <begin position="709"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="552"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="672"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="595"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="673"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="700"/>
            <end position="709"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF21400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR24699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="818" mass="94483" checksum="8AD83A08F47BD550" modified="2009-07-28" version="1">MPDVPSRPMDPHLHIKALVDTLAPRTAHQYKLYHTKFIQWCRDNKLLRRDPGADHPYRDIVVTATLVHCFVLSQYVCVSDSQFSASVLRKMISAFKFLHRVCCAYEPDYPYELDHDYLEAVARLHVSASAENPPFSPLHLVSVNMWTPHSNRLSEKYFKGGLERLRFLVDFHVQQYWHLPFADRARLKLGDLRFAADPGMLHVVRQGQHGADAPETVQQLALLPQRVPWICPLVSLAAYLYLRFYGAPKAYKGDGFPDLLGADEWAFLPMVRGKSLDKYPREETMTNYYAHVFRHCHLPYKRREYFYNKSVDYCQYPEIRRTELDQLQEIAPAQQRELFPHLIPLDFLRHMNHFPVYEPPSENHYADLANVPKSLLVQVFPEIEEYKRSSIPLGEQAQHFIDALQLLRSALVSALPLFYHFFPEHDLFKDPMFQNAEFQGYFHQKIEQLRATDQLQSYDPAAFGLVEAERDFAAPSQPPQALNVPPSADTQDLQIYLRDQTFRMVQFQTTSNFHLLLQSLSRIFEKLETKKSNREYIIHQLGSLEQTLQDQIAASKPQDVKTEEETSSSSTNGPKQETRESLPAGKLSRPAVFDSDAEDDDDYKEEDDNEDNDDEVDPNLQNELQALVSQVMDTRFKSAVEQQTAQIERHVQSLISAQVKEEVRKQLAHLLNQQSSTPTPQPSQQPVTIAPKRPREEQEPPSTAGESTFAMSPQLTSIEDIILEWFTPNPEQGNECVHTMNKKFGKTWRAGSQEATHLYKQRKLIIEFYICLVNQRQMDRYEAVAVCEKLRDNASIQEFSNMLKSWKKGHNNSFDGLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLP5</accession>
    <name>C5DLP5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0G02398p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0G02398g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555143.1">
        <property type="nucleotide sequence ID" value="XM_002555097.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLP5"/>
    <dbReference type="FunCoup" id="C5DLP5">
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    </dbReference>
    <dbReference type="STRING" id="559295.C5DLP5"/>
    <dbReference type="GeneID" id="8293405"/>
    <dbReference type="KEGG" id="lth:KLTH0G02398g"/>
    <dbReference type="eggNOG" id="KOG0152">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039649_0_0_1"/>
    <dbReference type="InParanoid" id="C5DLP5"/>
    <dbReference type="OMA" id="EPTKYHY"/>
    <dbReference type="OrthoDB" id="410044at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.440">
        <property type="entry name" value="FF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002713">
        <property type="entry name" value="FF_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036517">
        <property type="entry name" value="FF_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039726">
        <property type="entry name" value="Prp40-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11864">
        <property type="entry name" value="PRE-MRNA-PROCESSING PROTEIN PRP40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11864:SF30">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR URN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01846">
        <property type="entry name" value="FF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00441">
        <property type="entry name" value="FF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81698">
        <property type="entry name" value="FF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WW" evidence="2">
        <location>
            <begin position="4"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="2">
        <location>
            <begin position="59"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="178"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="284"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="159"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="180"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="192"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="293"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR24706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="462" mass="53218" checksum="D2AD89AE3A5848CC" modified="2009-07-28" version="1">MHPRKMPRYWREYKTPDGTKYYYNVNTKRSTTDKPDDFEESQSFRSKSRRVDPGVTFVVPLSNDWVLSVCENGQRFFYKSSTEESRWDIEDEESLRILSLVDKNKLVLLLATARGFDIGERDVYQELLEDINNLKQSRGVPEGSEVIEAAEEPSHTAGEPSNATEEPSQAVRTLISAYESSDEDGDSSDEINAEKEVHSDNEGSNSDIEDLNAASHQYNEATKQMFTSLFDEYDLDPYSTWNMQSKKICDNPLFYQVASDAERRELFEDWCVAKVKLEDVIEDPAHQAVSSDGDGDDDDTSTDELEPTRYHYLAHIVSKAEVAPSTLPRDLRSEQKQLFKQFKIKESLGKKQQDEFLSKLLFYYKNMTEEQRLEVLVKFLKTRRKLIMKGFRNIEQLESVLSQQDLPTSGFAIESQLLRLEDCIDIHGVSKALQDEVQYYVLGIKQKTTALKEFLKQLNSEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLX3</accession>
    <name>C5DLX3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 12">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="11 12">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0G04246g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Essential for the maintenance of cell size and integrity in response to osmotic stress.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 12">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 11">Belongs to the PPP phosphatase family. PP-Z subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="11 12"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555221.1">
        <property type="nucleotide sequence ID" value="XM_002555175.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLX3"/>
    <dbReference type="FunCoup" id="C5DLX3">
        <property type="interactions" value="380"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLX3"/>
    <dbReference type="GeneID" id="8293487"/>
    <dbReference type="KEGG" id="lth:KLTH0G04246g"/>
    <dbReference type="eggNOG" id="KOG0374">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004962_3_0_1"/>
    <dbReference type="InParanoid" id="C5DLX3"/>
    <dbReference type="OMA" id="SFQYYEN"/>
    <dbReference type="OrthoDB" id="1930084at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006883">
        <property type="term" value="P:intracellular sodium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07414">
        <property type="entry name" value="MPP_PP1_PPKL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000006">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050341">
        <property type="entry name" value="PP1_catalytic_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011159">
        <property type="entry name" value="PPPtase_PPZ/Ppq1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031675">
        <property type="entry name" value="STPPase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668">
        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668:SF484">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE PP-Z1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16891">
        <property type="entry name" value="STPPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000909">
        <property type="entry name" value="PPPtase_PPZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 11 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6 11" id="KW-0464">Manganese</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0519">Myristate</keyword>
    <keyword evidence="5 11" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="14">
        <location>
            <begin position="460"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="31"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="49"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="74"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="94"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="113"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="161"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="234"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="257"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="270"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="291"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="308"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00053877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR000909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR24784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="663" mass="72934" checksum="D7AFA3BEE7E17205" modified="2009-07-28" version="1">MGNTSSKVSSKKNKVSRDSAPPFARTDTSQSVRSGKSTRSVKSSKSRRSQSSQNAGASGSLDAAGPADPSRATAAADGSGSDAAIEQEPLRATGSFSRRNSVVSGLQPPLSKRGSSSSVFRESANGASTLQNKVHTATYASSTNGDLPPSMIQMAPKSPILKNSTSIGNLGPNTTYHENAVTDDENDENESHHHRRRSNDSRRYSQKSPVAGSRSNSSSSNRRKSGSHLELPDSSMTSVQPSRTSSHSSAGNHSRKSSFNSSSGNTAYSTPMNSPGVTKNSQDEYFEDSSEAQNDQSLQASRPRSRRNSIDEYPHEVSHSVDVDEDTNASITTKTIKRKKSLRPLDIDETIQKLLDAGYSGKRTKNVCLKNSEIAQICNQVREIFLSQPSLLELSPPVKIVGDVHGQYADLLRLFTKCGFPPSANYLFLGDYVDRGKQSLETILLLLCYKIKYPENFFLLRGNHECANVTRVYGFYDECKRRCNIKTWKIFIDTFNTLPLAAIVAGKIFCVHGGLSPVLNSMDEIRHVSRPTDVPDFGLINDLLWSDPTDSPHEWEDNERGVSYCYNKVAINKFLNKFGFDLVCRAHMVVEDGYEFFNDRSLVTVFSAPNYCGEFDNWGAVMSVSEGLLCSFELLDPLDSAALKQVMKKGRQERKLANQQQQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLY6</accession>
    <name>C5DLY6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">D-lactate dehydrogenase (cytochrome)</fullName>
            <ecNumber evidence="6">1.1.2.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">D-lactate ferricytochrome C oxidoreductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0G04532g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(R)-lactate + 2 Fe(III)-[cytochrome c] = 2 Fe(II)-[cytochrome c] + pyruvate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13521"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16004"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.1.2.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAD-binding oxidoreductase/transferase type 4 family.</text>
    </comment>
    <dbReference type="EC" id="1.1.2.4" evidence="6"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555234.1">
        <property type="nucleotide sequence ID" value="XM_002555188.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLY6"/>
    <dbReference type="STRING" id="559295.C5DLY6"/>
    <dbReference type="GeneID" id="8293500"/>
    <dbReference type="KEGG" id="lth:KLTH0G04532g"/>
    <dbReference type="eggNOG" id="KOG1231">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017779_3_3_1"/>
    <dbReference type="InParanoid" id="C5DLY6"/>
    <dbReference type="OMA" id="LMFGTDC"/>
    <dbReference type="OrthoDB" id="7786253at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004458">
        <property type="term" value="F:D-lactate dehydrogenase (cytochrome) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008720">
        <property type="term" value="F:D-lactate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903457">
        <property type="term" value="P:lactate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.45.10:FF:000001">
        <property type="entry name" value="D-lactate dehydrogenase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2740:FF:000001">
        <property type="entry name" value="D-lactate dehydrogenase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.45.10">
        <property type="entry name" value="Vanillyl-alcohol Oxidase, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004113">
        <property type="entry name" value="FAD-bd_oxidored_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016164">
        <property type="entry name" value="FAD-linked_Oxase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016171">
        <property type="entry name" value="Vanillyl_alc_oxidase_C-sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11748:SF117">
        <property type="entry name" value="AER321WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11748">
        <property type="entry name" value="D-LACTATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02913">
        <property type="entry name" value="FAD-oxidase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55103">
        <property type="entry name" value="FAD-linked oxidases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="42"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="10">
        <location>
            <begin position="134"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00051436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00083446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR24797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="572" mass="62748" checksum="8CC6744B4594D677" modified="2009-07-28" version="1">MFSRCRFVIKGGSRFSTKTSLTNASRSGIRLFSTSPISGPKFLGSAIGFALLSFGLGLAVSSLVIPKQDAQVQTFPLASTTPLEQLDSPRYGSEEDLEKCVDEIRRVVGDELVKNTPVEIDYHTDNGFNPSKPLPHQKPRYIVYPLSTEDVSKIMKIVNQYNIPVVPYSGGTSLEGHTYSTRPGIVLNTSKMNKILQVNVDDLDAVLQAGVGWQDLNEYLSKHEGMDNLMLGCDCGPGAHVCGMISTNASGIGATRYGSMASNVVSIKAVLADGTVIKTKLRPRKSSAGYNLTGLLVGSEGTLAIVTEVVVKLQVKPGYETVAVVQFPDVNDCTKSMASFFKKGIPLNAVELLDADMMRCVNYSDLVSKKFENLPTLFIKIGGLNKVIVDEYVKEVSAISKSNNCNTFEFARTAEEAEELFFARKNALYIMLDYGFNEISPDAKMWITDCAVPLSRLASTLEQLNDMMKEYPLHHMVLGHGDANLHYDIFYTPDQLEMCKEAVDRMAKLTIENEGTCSGEHGIGSGKRALLELELGKDTISMMRKLKLALDPKRLLNPDKVFKIDPLDASTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5DM11</accession>
    <name>C5DM11_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0G05082p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0G05082g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555259.1">
        <property type="nucleotide sequence ID" value="XM_002555213.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DM11"/>
    <dbReference type="FunCoup" id="C5DM11">
        <property type="interactions" value="731"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DM11"/>
    <dbReference type="GeneID" id="8293525"/>
    <dbReference type="KEGG" id="lth:KLTH0G05082g"/>
    <dbReference type="eggNOG" id="KOG1443">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022332_1_1_1"/>
    <dbReference type="InParanoid" id="C5DM11"/>
    <dbReference type="OMA" id="WLMKSFP"/>
    <dbReference type="OrthoDB" id="18894at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004853">
        <property type="entry name" value="Sugar_P_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03151">
        <property type="entry name" value="TPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="5"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="101"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="132"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="163"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="212"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="250"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="284"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="312"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="Sugar phosphate transporter" evidence="6">
        <location>
            <begin position="5"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR24822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="370" mass="41406" checksum="F4170D551D8CFA4D" modified="2009-07-28" version="1">MARTILGYIAGWYVCSIALSLYNKWMFDPERGLSVPYPILVTMMQQWILWGISWVYLKSKGIEQVVEQSRQQRWKFYLKFIIPAALATAGDIGLANVSFKFITLTIYTIVKSSSIAFVLLFGCLFRTEMFHWRLACIVAVMFAGVAMMVYRPRSDGGGPADEHIVLGVMLVLGSSMLSGLRWVYTQLVLRKVDANVDPSVGEKKKKSPVETIHQLTPVMGLALLVTTLAVEKPFPAVFHSPLLQIEDHTSALSLVRGFFLLLTPGVLVFLLVVCEFGILQHAQVLTLSVAGVCKEVITILASMLVLRETLSGFQNWLGMGVILLDVCYYNFYRFSQKKGASQESSDDASEVLLQEFELESNKQIPELQKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DM81</accession>
    <name>C5DM81_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Inositol-1-monophosphatase</fullName>
            <ecNumber evidence="10">3.1.3.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0G06710g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>a myo-inositol phosphate + H2O = myo-inositol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:24056"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:84139"/>
            <dbReference type="EC" id="3.1.3.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 9 10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 10">Polyol metabolism; myo-inositol biosynthesis; myo-inositol from D-glucose 6-phosphate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the inositol monophosphatase superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.25" evidence="10"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555329.1">
        <property type="nucleotide sequence ID" value="XM_002555283.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DM81"/>
    <dbReference type="FunCoup" id="C5DM81">
        <property type="interactions" value="463"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DM81"/>
    <dbReference type="GeneID" id="8293598"/>
    <dbReference type="KEGG" id="lth:KLTH0G06710g"/>
    <dbReference type="eggNOG" id="KOG2951">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044118_1_2_1"/>
    <dbReference type="InParanoid" id="C5DM81"/>
    <dbReference type="OMA" id="FNVMKPD"/>
    <dbReference type="OrthoDB" id="10254945at2759"/>
    <dbReference type="UniPathway" id="UPA00823">
        <property type="reaction ID" value="UER00788"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008934">
        <property type="term" value="F:inositol monophosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006021">
        <property type="term" value="P:inositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071545">
        <property type="term" value="P:inositol phosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01639">
        <property type="entry name" value="IMPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.540.10:FF:000013">
        <property type="entry name" value="Inositol-1-monophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.80:FF:000012">
        <property type="entry name" value="Inositol-1-monophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.10">
        <property type="entry name" value="Fructose-1,6-Bisphosphatase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033942">
        <property type="entry name" value="IMPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020583">
        <property type="entry name" value="Inositol_monoP_metal-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020550">
        <property type="entry name" value="Inositol_monophosphatase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20854">
        <property type="entry name" value="INOSITOL MONOPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20854:SF4">
        <property type="entry name" value="INOSITOL-1-MONOPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00377">
        <property type="entry name" value="IMPHPHTASES"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56655">
        <property type="entry name" value="Carbohydrate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00629">
        <property type="entry name" value="IMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00630">
        <property type="entry name" value="IMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0452">Lithium</keyword>
    <keyword evidence="8 9 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 9 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR600760-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR24892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="292" mass="32243" checksum="8528A33B69EE9DCF" modified="2009-07-28" version="1">MSLDLRSIEDYLCSLAKEKVGPIIKAKSGTQHVYDAKSGSRAVDIVTAIDKQVEKLIWTNVKEKYPDFKFVGEESFVPGETTITDEPTFIIDPIDGTTNFVHDFPFSCTSLGLALNKEPVVGVIYNPHLDLLVSASKNNGVRVNGKTFDFSAKTKEMGPLTLKTSVVALQPGSAREGSNFQTKMRTYEALLSCDEGFVHGFRNLGSSAMTLAYICLGYLDSYWDGGCYSWDVCAGWCILKETGGRIVGANPGEWDVPVDNRTYLAVRACESSKQQEQYIEDFWACVKGSLKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DM89</accession>
    <name>C5DM89_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0G06886p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0G06886g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555337.1">
        <property type="nucleotide sequence ID" value="XM_002555291.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DM89"/>
    <dbReference type="FunCoup" id="C5DM89">
        <property type="interactions" value="666"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DM89"/>
    <dbReference type="GeneID" id="8293606"/>
    <dbReference type="KEGG" id="lth:KLTH0G06886g"/>
    <dbReference type="eggNOG" id="KOG1685">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049748_0_0_1"/>
    <dbReference type="InParanoid" id="C5DM89"/>
    <dbReference type="OMA" id="SFYKPWK"/>
    <dbReference type="OrthoDB" id="10251727at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023674">
        <property type="entry name" value="Ribosomal_uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028364">
        <property type="entry name" value="Ribosomal_uL1/biogenesis"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00687">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56808">
        <property type="entry name" value="Ribosomal protein L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="13"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="408" mass="45155" checksum="79723404D254B64A" modified="2009-07-28" version="1">MAKSTPVKKRGGSKSTTPVSTPPVKTKKESRSGTPASTAGSTPVSTPSKKKKRAKKPATSVKVQEAAKPKEPETVVPKDRINKAVQELSKFTSKKQEDSTQLLDDDDELDKQVELIAVNTGSFTGNRKVFKPKQFKIEHSIFKPWKKASETSVKDFKTLLILRDQDAVKVSEDEIYDQLRDSGITIDTVIPGADLKTKYKSFEKRRAFIQDFSLILADDSVVTTLPKLLGGKAYEKVSTTPVPIRTNKKGVFSLTILSNSIKKVFEHSLPAKMPRGTTLSVHLGNLNWFTADELTQNILTVVEQLVKEFQVRSVFLKSNKSPVLPLYYNQAVLDDLAKQTKEDSKDTATRKVTIDGTELELSAFEAALMEIANPDELDKVFAGRINRAKKRAAESEEESAPDSKKAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMB4</accession>
    <name>C5DMB4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0G07458p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0G07458g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555362.1">
        <property type="nucleotide sequence ID" value="XM_002555316.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMB4"/>
    <dbReference type="FunCoup" id="C5DMB4">
        <property type="interactions" value="716"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMB4"/>
    <dbReference type="GeneID" id="8293632"/>
    <dbReference type="KEGG" id="lth:KLTH0G07458g"/>
    <dbReference type="eggNOG" id="KOG2863">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005893_1_0_1"/>
    <dbReference type="InParanoid" id="C5DMB4"/>
    <dbReference type="OMA" id="KWWFSAH"/>
    <dbReference type="OrthoDB" id="407609at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008419">
        <property type="term" value="F:RNA lariat debranching enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007708">
        <property type="entry name" value="DBR1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12849:SF0">
        <property type="entry name" value="LARIAT DEBRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12849">
        <property type="entry name" value="RNA LARIAT DEBRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05011">
        <property type="entry name" value="DBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="2">
        <location>
            <begin position="23"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Lariat debranching enzyme C-terminal" evidence="3">
        <location>
            <begin position="298"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="263"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="273"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR24925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="386" mass="44173" checksum="669899B744543674" modified="2009-07-28" version="1">MNVAIGVLSKKKECNYRAVMTHLRIAVQGCAHGELVNIYNHVARQYASEMPDILIVLGDFQSLRDEDDMDSIAVPDKYKKLGDFPKYFTGELEAPLLTIFIGGNHENMRGLAELPRGGFVAKNIYYMGYSGSIVVQGVRISGLSGIYKSHDFERPRASLSQIKAEGWGRHVRNMYHVRKTDVLPLFMLTQTDIMLSHDWPNGIAHHGDLKKLLKQKPFFKRDIFNNDLGSPVNWQLLRKLTPCWWLSAHLHVKFEAQVALNKRARNESTENTDSNEIDLGLDDDEDDGDDDANIETSEKTNFLALDKCGRNRKHMEIITVEADTSHPTFHPEAVQIYVDPEFVANQQFLEKNPPKSRLEHISFESLRERRGELGEIDWESYRLNYA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-05-03" modified="2025-06-18" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMC1</accession>
    <name>NOP9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName>Nucleolar protein 9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Pumilio domain-containing protein NOP9</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">NOP9</name>
        <name type="ordered locus">KLTH0G07612g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">RNA-binding nucleolar protein required for pre-rRNA processing. Involved in production of 18S rRNA and assembly of small ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NOP9 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555369.1">
        <property type="nucleotide sequence ID" value="XM_002555323.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMC1"/>
    <dbReference type="SMR" id="C5DMC1"/>
    <dbReference type="FunCoup" id="C5DMC1">
        <property type="interactions" value="992"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMC1"/>
    <dbReference type="GeneID" id="8293639"/>
    <dbReference type="KEGG" id="lth:KLTH0G07612g"/>
    <dbReference type="eggNOG" id="KOG2188">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008720_1_1_1"/>
    <dbReference type="InParanoid" id="C5DMC1"/>
    <dbReference type="OMA" id="HHLVRNF"/>
    <dbReference type="OrthoDB" id="392571at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000056">
        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040000">
        <property type="entry name" value="NOP9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13102">
        <property type="entry name" value="NUCLEOLAR PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13102:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22493">
        <property type="entry name" value="PUF_NOP9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0539">Nucleus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0690">Ribosome biogenesis</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <feature type="chain" id="PRO_0000407815" description="Nucleolar protein 9">
        <location>
            <begin position="1"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio 1">
        <location>
            <begin position="89"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio 2">
        <location>
            <begin position="125"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio 3">
        <location>
            <begin position="189"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio 4">
        <location>
            <begin position="289"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio 5">
        <location>
            <begin position="339"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio 6">
        <location>
            <begin position="376"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio 7">
        <location>
            <begin position="517"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio 8">
        <location>
            <begin position="555"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="637"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="651"/>
            <end position="670"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="670" mass="77088" checksum="DC7D9A3DBCD7130A" modified="2009-07-28" version="1">MGKPRGRKLLKKHHTEGEEASHVEPQPYDTNDTIPEKISSGSNGEPNTFFGVLDTQELEYFKQAESTLAVDTFENAEEKEQFISSVIEETKNKELKLATSQICSKLMERIILAGSNQQLKAIFRSFNGFFYGLACQKYSSHVLETLFVRGAAMVEKELLTPSFDEPGATEDGDDVYSPMEDLFLYMLNEITPSVRGMISHKYASHVFRLLILILSSKTLPSTTMSNSALRSKRSKIARKMIDLKDNEDFDKTYKTPDSFKVKLREILSSLYKELTGGVDLGSSNFKNISVTSMMKVRELCIDPVASPVLQLIIQVEGIFDRDRSFWHLIFSSSEEKNAKEEAFVEYLLSDPVGSHFLENVISFTRAKYTERLYKMYMRDRILKLSKRDLTGAFVVQALLKHLRQNDVKSILNDIVPELSLIVNSNMEFCISILDASIQHGDYLKEEIVLQLIKKFYPEGLEDRNILESCLSLNSSTLGNTRDDWPTAEERRRSLFLEKLIDYDDRFLNITIESLLALPEERLLQMCYHGVFSHVVEHVLQVKRVDKIKRKRLLNVFCTDIVNLSCNAYGSHLVDKLWDFTAQLPMYKERIATSMAGDSEKVKNSVYGRQAWKNWSMDKFLRKQFDWKRLIREQQQELFPDATPLQPGGDAGTKRKGDKPSYNRPEKKFRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMF4</accession>
    <name>C5DMF4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0G08426p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0G08426g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the XPG/RAD2 endonuclease family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555402.1">
        <property type="nucleotide sequence ID" value="XM_002555356.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMF4"/>
    <dbReference type="FunCoup" id="C5DMF4">
        <property type="interactions" value="415"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMF4"/>
    <dbReference type="GeneID" id="8293675"/>
    <dbReference type="KEGG" id="lth:KLTH0G08426g"/>
    <dbReference type="eggNOG" id="ENOG502QVHA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_378548_0_0_1"/>
    <dbReference type="InParanoid" id="C5DMF4"/>
    <dbReference type="OMA" id="RFYQTKV"/>
    <dbReference type="OrthoDB" id="17262at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09858">
        <property type="entry name" value="PIN_MKT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022039">
        <property type="entry name" value="MKT1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022040">
        <property type="entry name" value="MKT1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006086">
        <property type="entry name" value="XPG-I_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006084">
        <property type="entry name" value="XPG/Rad2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006085">
        <property type="entry name" value="XPG_DNA_repair_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081">
        <property type="entry name" value="FLAP ENDONUCLEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081:SF32">
        <property type="entry name" value="POST-TRANSCRIPTIONAL REGULATOR MKT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12246">
        <property type="entry name" value="MKT1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12247">
        <property type="entry name" value="MKT1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00752">
        <property type="entry name" value="XPG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00484">
        <property type="entry name" value="XPGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00485">
        <property type="entry name" value="XPGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="XPG N-terminal" evidence="4">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="XPG-I" evidence="3">
        <location>
            <begin position="184"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR24965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="804" mass="90600" checksum="E198C5747749A548" modified="2009-07-28" version="1">MPVKSLESFLFERGLVGTYPIESLKNATLGIDVDHYVSRLLTSKREQYLDAIGGFPTSLKLYLESDLQVFREFDITPIFVFSGSPVANQLEANGHYAAAAPEAAAAAINASNSGPMSSSATSTTRTAKEAILAQRHKGWTQWNNMHANNQSSYIDQPVPPSEPFKYSIPLDTKRFQSDLIDYFISLDIRFQVAPYSSWFQLTYLLQNEYIDAIYGPTELLMLKSVEKFILGMEFPNKEYRFVDRARVLSEINCSPEEFVDICMTVGNDLQPYTLSPLRMYPVQQLFEVALEMVLTSGTDFYAYQLMNPIKSESIQSVARYQKGISALKFMPVLKENGKVELYSPAERGEDRMTYSAGSPYSPASSTGSLESIPNDVHDVVAQRLPHEYYFYQSIGLVSGKLMDAITTGVYPDEPPLDGGSSESYRKLVKNSVELFKNKEINLLTQPINRYYQIKPIKHVKWFAPDDAVTLANRVTPPIFDRINHLIVKISQPEREFSLAQFLTMVNESGDLTKSFVSDKVLFPNSVPLNEKLATPFDVLATAFIRFLSLLGFFDFDSSQKTLKVTPWGRILLDMTKTGVDSESIEPVFVLLMFLKTGVLSLREETVPATPSALSKATLRSYSKESSYILVLTRILSIFRLQQKPSNYHGPIDRKTLVFRDHLDYVKENLNDLFEVTLVSSLAAGEFDRLSLDNTGWQKKIVAAMPFKLSTPSTVMAMIWQFYLQKFLHNGNSKSDALNLIASTFNTSKVVPNLDEELKRSLDFARQFGQVLGALSKANLCEKQDYELVVEAVEFAEKAFADERV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMG5</accession>
    <name>C5DMG5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0G08734p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0G08734g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555413.1">
        <property type="nucleotide sequence ID" value="XM_002555367.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMG5"/>
    <dbReference type="STRING" id="559295.C5DMG5"/>
    <dbReference type="GeneID" id="8293689"/>
    <dbReference type="KEGG" id="lth:KLTH0G08734g"/>
    <dbReference type="eggNOG" id="KOG1003">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104738_1_1_1"/>
    <dbReference type="InParanoid" id="C5DMG5"/>
    <dbReference type="OMA" id="EQWETKY"/>
    <dbReference type="OrthoDB" id="128924at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000001">
        <property type="entry name" value="Tropomyosin alpha-1 chain isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000533">
        <property type="entry name" value="Tropomyosin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19269">
        <property type="entry name" value="TROPOMYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00261">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="27"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="27"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="59"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="98"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR24976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="161" mass="19228" checksum="83644975248063E5" modified="2009-07-28" version="1">MDKIREKLSNLKLEAESWQEKYEEVKEQMKQLEQENTEKENQIKSLTTKNQQLEDEVEKLEGELSEHKQLSEDSHNLRSHNENFTKKNQQLEEELEESDTKLKETTDKLRETDLKAEGLERKVVSLEQERDDWEKKYEELTAKYDEAKKELDEIAASLENL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMR9</accession>
    <name>C5DMR9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 15">Chromatin-remodeling ATPase INO80</fullName>
            <ecNumber evidence="15">3.6.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ordered locus">KLTH0G11132g</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">ATPase component of the INO80 complex which remodels chromatin by shifting nucleosomes and is involved in DNA repair.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Component of the INO80 chromatin-remodeling complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="15">The DBINO region is involved in binding to DNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.-" evidence="15"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555517.1">
        <property type="nucleotide sequence ID" value="XM_002555471.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DMR9">
        <property type="interactions" value="1205"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMR9"/>
    <dbReference type="GeneID" id="8293796"/>
    <dbReference type="KEGG" id="lth:KLTH0G11132g"/>
    <dbReference type="eggNOG" id="KOG0388">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000315_26_2_1"/>
    <dbReference type="InParanoid" id="C5DMR9"/>
    <dbReference type="OMA" id="FWKKNER"/>
    <dbReference type="OrthoDB" id="372624at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051276">
        <property type="term" value="P:chromosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010557">
        <property type="term" value="P:positive regulation of macromolecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000022">
        <property type="entry name" value="Blast:Putative DNA helicase Ino80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001269">
        <property type="entry name" value="SNF2 family helicase/ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020838">
        <property type="entry name" value="DBINO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050520">
        <property type="entry name" value="INO80/SWR1_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45685:SF2">
        <property type="entry name" value="CHROMATIN-REMODELING ATPASE INO80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45685">
        <property type="entry name" value="HELICASE SRCAP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13892">
        <property type="entry name" value="DBINO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51413">
        <property type="entry name" value="DBINO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0010">Activator</keyword>
    <keyword evidence="7 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 15" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12 15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9 15" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="DBINO" evidence="19">
        <location>
            <begin position="372"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="17">
        <location>
            <begin position="611"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="18">
        <location>
            <begin position="1179"/>
            <end position="1333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="107"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="314"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="443"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="107"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="136"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="151"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="168"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="189"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="229"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="443"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU368001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CAR25080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1339" mass="155569" checksum="2805FFC69AE2FA40" modified="2009-07-28" version="1">MSLAALLNEDDANPSQRQSFVKDLNQEFRALGARDIKEQRYQDWKMLSYQEFELVTEWNNQSRELGSNGCEKLISNMDSTRREWEQFQEYAARKEEVIRQQRELRLKQQQEQEQERLQQEKERQETLEQERHQKKAQAAQAAQAAQSQNQHGDRSRVAIKSEPKDNKTLNGNSNPQSRSTDFDGHGDGAEDENEDDEEIDEDEDEEAEIEEEPEHDEEVLFPDDDDNDKDFYLEDEKGEFPSKSKKVDLSSERAKIRREIARMVNKNKNVKAKKRRFTNSTIIEYVSDEKLEVKLTLKQYHVRQLKRLLAENKRAQEEDANESTAKKRKVGGKGDEAQIPSSIEGLPTYGLKMTIKEARGIQRHYDTTYFTIWKDMARKDSAKVARLVQQTQSSRNVNFKKTSSLVAREARKWQLRNFRQLKDFQTKARRGVREMSSFWKKNEREERDLKKKAEKEALDQAKKEEEERENKRQARKLNFLITQTELYSHFIGRKIKTDEIEGSMADSNLKQANGGGHNIDLSKTQAAKTDFHSIDFDNEDDENLRLKAAQNASSALLATQAKAREFDSNPDEEDELNFQNPTSLGEITIDQPRLLTCTLKEYQLKGLNWLANLYDQGINGILADEMGLGKTVQSISVLAHLAENHNIWGPFIVVTPASTLHNWVNEISKFLPDFKILPYWGNGNDRKILRRFWDRKQFRYGKDAPFHVMVTSYQMVVSDAAYLQKMKWQYMILDEAQAIKSSQSSRWKNLLSFHCRNRLLLTGTPIQNSMQELWALLHFIMPSLFDSHDEFSDWFSKDIESHAQSNTKLNQQQLRRLHMILKPFMLRRIKKNVQSELGEKIEIDVMCDLTRRQNKLYQVLRSQMSAAYDAIENAAGNDEASSDQNIVNTVMQFRKVCNHPDLFEREDVRSPLAFTNFGKSGSLLREGDLIDVDYSARNSITYQIPRLVFDELIAPNFQNDSDVRNKILNHTFNIFHPSNSRSLCMTLSCLTGYSQGEIKDLSHQPIVQRALKLNEDKLSEGSKLLSVYDDKTQLRELNKNSFIVEDERRNLVTLSKSSTKGVLDSLLNIKQGVYEDEYMNSWQPAYHPAVSAPPINLEVSNSNYFSHEKHLELFDPFLCQALNRIPAQTQYELRTKFQLPVSQFPISECYPEALNKNFSSAISMPSMDRFITESTKLKKLDELLVDLKKGDHRVLIYFQMTKMMDLMEEYLTYRQYKHIRLDGSSKLEDRRDLVHDWQTKPDIFIFLLSTRAGGLGINLTAADTVIFYDSDWNPTIDSQAMDRAHRLGQTRQVTVYRLLIRGTIEERMRDRAKQKEHVQQVVMEGKTQDKDIQTVDASG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMV7</accession>
    <name>C5DMV7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0G12034p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0G12034g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555555.1">
        <property type="nucleotide sequence ID" value="XM_002555509.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMV7"/>
    <dbReference type="FunCoup" id="C5DMV7">
        <property type="interactions" value="54"/>
    </dbReference>
    <dbReference type="GeneID" id="8293837"/>
    <dbReference type="KEGG" id="lth:KLTH0G12034g"/>
    <dbReference type="eggNOG" id="KOG3511">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_994238_0_0_1"/>
    <dbReference type="InParanoid" id="C5DMV7"/>
    <dbReference type="OrthoDB" id="4066654at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110296">
        <property type="entry name" value="Oligoxyloglucan reducing end-specific cellobiohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950552" evidence="1">
        <location>
            <begin position="18"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR25118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="280" mass="31107" checksum="182B78CBDAF30391" modified="2009-07-28" version="1">MLASRLLALTCVGLTLAAGQPRVSLTDDLRVSAFAGSADLWRLNNGSVSVSTDGGANWATVDSLGHDIESFKIDENFPESRAFAIDSKRGRIFQSVDQGRAWTEMLSDASFWDNPEDEANPLASLWFSMQTNPYAREDVVVTLSQCLENEYVRRCNASKNAFISTDGEYFRRATEIAAPKGQLLDNRCRFFGAEAGPRVICQLRLSQQPQGEGYQGLNVKLFYSDDHGETFAALEEFEHRSVMSSMTFGGLDAVTTIEAKKSHQQSAYQLWLSRDGSHYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>C5DN05</accession>
    <name>C5DN05_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0G13156p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0G13156g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555603.1">
        <property type="nucleotide sequence ID" value="XM_002555557.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DN05"/>
    <dbReference type="FunCoup" id="C5DN05">
        <property type="interactions" value="12"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DN05"/>
    <dbReference type="GeneID" id="8293886"/>
    <dbReference type="KEGG" id="lth:KLTH0G13156g"/>
    <dbReference type="eggNOG" id="ENOG502SBHE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139325_0_0_1"/>
    <dbReference type="InParanoid" id="C5DN05"/>
    <dbReference type="OMA" id="CEYDEYI"/>
    <dbReference type="OrthoDB" id="5555533at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070096">
        <property type="term" value="P:mitochondrial outer membrane translocase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045040">
        <property type="term" value="P:protein insertion into mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037652">
        <property type="entry name" value="Mim2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28230">
        <property type="entry name" value="CHROMOSOME 1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28230:SF1">
        <property type="entry name" value="MITOCHONDRIAL IMPORT PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19117">
        <property type="entry name" value="Mim2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR25166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="108" mass="12821" checksum="E9D9C8BA70A53849" modified="2009-07-28" version="1">MSSVWDEIRRQEELSEQMLLQAAEAGGEAVEISGDEQEYDYDCEYDEYIVDSDEEDYEAQLLSAQQQWEESLEQLSQVFNWILLPLVGKYLGRRTALRVWKNVMQYIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DN33</accession>
    <name>C5DN33_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Actin-like protein ARP6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Actin-like protein arp6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0G13772g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the SWR1 complex which mediates the ATP-dependent exchange of histone H2A for the H2A variant HZT1 leading to transcriptional regulation of selected genes by chromatin remodeling. Involved in chromosome stability.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the actin family. ARP6 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555631.1">
        <property type="nucleotide sequence ID" value="XM_002555585.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DN33"/>
    <dbReference type="FunCoup" id="C5DN33">
        <property type="interactions" value="382"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DN33"/>
    <dbReference type="GeneID" id="8293914"/>
    <dbReference type="KEGG" id="lth:KLTH0G13772g"/>
    <dbReference type="eggNOG" id="KOG0680">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027965_1_1_1"/>
    <dbReference type="InParanoid" id="C5DN33"/>
    <dbReference type="OMA" id="FFEEYEC"/>
    <dbReference type="OrthoDB" id="6220758at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10210">
        <property type="entry name" value="ASKHA_NBD_Arp6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000040">
        <property type="entry name" value="Actin-like protein ARP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00073820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR25194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="420" mass="47923" checksum="3A37F99FD27319C0" modified="2009-07-28" version="1">MEEAPIVIDNGSYEIKFGTASSNEPFTALNSIAKDKYGNYHLSNQVRSIKDISSVTFNRPHKLGQLVSWELESQIWDYCLFNPDEFDGWSLDTTKNKHLIASESCMTLPELSKNMDQVVFEEYEYDSLFKAPVPVFVPFNAEGDTTHILVAKEDADVVASDHPSDNKDYRDYELVIDSGFNCTWVVPVIKGVPYYKAVKKLDIGGRFLNGFLREALSFRHYNVMDETILVNSIKEKCLFMSPNSYFDSFQSRLKTAKEYVLPDFSTSFQGYLRKPGQTLPEDSQTIILQDELFAVPETFFHPEIASLLNPGIIEAILESISMLPEALRPLLVGNVVVTGGNFNIPNFASRLATELQRQCPTDWSCRVSVPTGDARLYGWKCMQKFSETQAYRESRVSREEYYEHGVDWCTSKRFGYQAWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNB0</accession>
    <name>C5DNB0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0G15532p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0G15532g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555708.1">
        <property type="nucleotide sequence ID" value="XM_002555662.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNB0"/>
    <dbReference type="STRING" id="559295.C5DNB0"/>
    <dbReference type="GeneID" id="8293994"/>
    <dbReference type="KEGG" id="lth:KLTH0G15532g"/>
    <dbReference type="eggNOG" id="ENOG502RDU8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_660685_0_0_1"/>
    <dbReference type="InParanoid" id="C5DNB0"/>
    <dbReference type="OMA" id="DIPHEQD"/>
    <dbReference type="OrthoDB" id="2138282at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055343">
        <property type="entry name" value="CREG_beta-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37273:SF1">
        <property type="entry name" value="ADL397C-AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37273">
        <property type="entry name" value="CHROMOSOME 8, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13883">
        <property type="entry name" value="CREG_beta-barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002948850" evidence="2">
        <location>
            <begin position="20"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="CREG-like beta-barrel" evidence="3">
        <location>
            <begin position="27"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="199"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="355"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="243"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="274"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="362"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="388"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR25271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="416" mass="46415" checksum="70F77B3EA5B4F153" modified="2009-07-28" version="1">MKLFALATALAAGLQVAGAEREHKTLDAHSAARFARRLALKERDAHANTIDRETGDPVSSVEYVVGSDACHGVTTSQPGNILLLFVNASSTYQNWRDNSRFSLTLEKHHGRRPVPPLAAPRANFFGELKPVERSDALDQCFLRKHPDAEHWLPGSKHHDVDDGQWYELDVSRVNFVGGFGKHGFAGEISGDEYHNAVGNDTEWASRGHGGDDGDEESSESLFSIAHLRERIRGLLRADDGHKGERRAKGHHAKGGHAKGYHAEGRAKNHRAKGHAKDHAEYHAKDYAEDRAEDRAEDHAEDHAEDHAEDRHVKNHPKAQPEEQRGVARLSPDGVRQIGVALRGFHGEYYIQYDPELNGRQVGEQDARDEQDARDEQDIPHEQDASSKRREHKAKAKGKGHCKGAKKMHAKQRMHNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNC5</accession>
    <name>C5DNC5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">25S rRNA adenine-N(1) methyltransferase</fullName>
            <ecNumber evidence="4">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">BMT2</name>
        <name evidence="5" type="ordered locus">KLTH0G15862g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the N(1) position of an adenine present in helix 65 in 25S rRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
            <location evidence="4">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the BMT2 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="4"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555723.1">
        <property type="nucleotide sequence ID" value="XM_002555677.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNC5"/>
    <dbReference type="FunCoup" id="C5DNC5">
        <property type="interactions" value="114"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNC5"/>
    <dbReference type="GeneID" id="8294009"/>
    <dbReference type="KEGG" id="lth:KLTH0G15862g"/>
    <dbReference type="eggNOG" id="ENOG502R82D">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041583_1_0_1"/>
    <dbReference type="InParanoid" id="C5DNC5"/>
    <dbReference type="OMA" id="NNSRYMN"/>
    <dbReference type="OrthoDB" id="5954793at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016433">
        <property type="term" value="F:rRNA (adenine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03044">
        <property type="entry name" value="BMT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021867">
        <property type="entry name" value="Bmt2/SAMTOR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21008:SF1">
        <property type="entry name" value="25S RRNA (ADENINE(2142)-N(1))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21008">
        <property type="entry name" value="S-ADENOSYLMETHIONINE SENSOR UPSTREAM OF MTORC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11968">
        <property type="entry name" value="Bmt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="4">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR25286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="332" mass="38482" checksum="12B0FCD07D5A8BE0" modified="2009-07-28" version="1">MLSRRRKTVTGKLIVERPPAIKPSKARRIIRRFHLLINKRRIICNKIGIKLLEGDESTNSERISEKLRLNDTQSFYDEGWNAQNPSSEYEAQMLKIQRLDDDIQLFRILGYIMSEIHQRGGLQNYQLASSMGQDRNRGGDSSKILMNWFKTIRTPGHQYRALEIGSLSAENAISTSGVFDPVIRIDLNSSDPAKIKRQDFMKRPLPPSDAEKFDLISCSLVLNFVPTPQLRGAMLHRFQHFLRLDVEKTYIFLVLPLPCVSNSRYMSKTLLCDIMEYLGYKQSHAHESSKIIYMLFQRSFGQKVHEEQVVREFSKKVLIRDKANMNNFSIVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNJ2</accession>
    <name>C5DNJ2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0G17490p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0G17490g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555790.1">
        <property type="nucleotide sequence ID" value="XM_002555744.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNJ2"/>
    <dbReference type="FunCoup" id="C5DNJ2">
        <property type="interactions" value="94"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNJ2"/>
    <dbReference type="GeneID" id="8294083"/>
    <dbReference type="KEGG" id="lth:KLTH0G17490g"/>
    <dbReference type="eggNOG" id="ENOG502R9EV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_348554_0_0_1"/>
    <dbReference type="InParanoid" id="C5DNJ2"/>
    <dbReference type="OMA" id="ERGMYRY"/>
    <dbReference type="OrthoDB" id="9994419at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR25353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="726" mass="83496" checksum="B08139E177CE622C" modified="2009-07-28" version="1">MILPPEIVYEVLTYQFQDLMSNDHPNNPEKFHSNLRIFLRSNLTVNKTFYHVCKVLIYRYLNFTSARGFYKLLETLRQSPELVRYVQVADFEELTSIGLGRTGEMNKSIKNLTNETLWEFLWLTRGSLREFLASEHIQGDLDERIINFLLKPGTILSVVDFCGSSHDKFTETFTGAVNKLYRDGQPIDYNYQITSLGLNDCTTIASSTLGKLLATLPELQKLDLGHTSIDDRTIINSLPHLKGLTHLSLSMCSQLTPRGVLEFFSYHPAVTDVDNSTTLQWLNVQVHPHSSSWSDVQLMFLLKRLCRYGHNKTLQYLNIGGLPLHLTEDLSVTKTMYYWQCQDTLHFIKLNFPHLKSLSIKDNNVPVPRLVSFLSPLQSEEIAEQRLKFLNVVNNAYINRWSIQDPTILVHSKSLCAIELSFDPWQQIEASNSRHEINCRLIDNTTLLQDPSKSQPVTWKCYIGSVYGRRHWLYKTDEYLNRGDIDTLGYLTNYDSQGNKIIDIFKQPDFLKFAQTKIMLGCGIVRQSSARRNLTYRDLNAPISQFFDKNRTIARRPNTPPIRIPVRPPGEWRLIDNENPLQEGEVHGGNLLNDNTLHSPDTSDAPPNSRIGLFWDRSMSNLSQSMQAYESDLQYLNDPELQRRRSQLSLFRGNFIADKDSLGGPQHRLASASFHTDSSIRDTTICDGNEAPNMTMAYRQHLEVSAEYTVFGCIERGMYRYYSLKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNR2</accession>
    <name>C5DNR2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">KLTH0G19162p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0G19162g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CCDC90 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555860.1">
        <property type="nucleotide sequence ID" value="XM_002555814.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNR2"/>
    <dbReference type="FunCoup" id="C5DNR2">
        <property type="interactions" value="280"/>
    </dbReference>
    <dbReference type="GeneID" id="8294155"/>
    <dbReference type="KEGG" id="lth:KLTH0G19162g"/>
    <dbReference type="eggNOG" id="KOG3156">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063283_2_0_1"/>
    <dbReference type="InParanoid" id="C5DNR2"/>
    <dbReference type="OMA" id="MAYMRFR"/>
    <dbReference type="OrthoDB" id="889336at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000018">
        <property type="entry name" value="Mitochondrial protein FMP32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024461">
        <property type="entry name" value="CCDC90-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14360">
        <property type="entry name" value="PROTEIN FMP32, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14360:SF1">
        <property type="entry name" value="PROTEIN FMP32, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07798">
        <property type="entry name" value="CCDC90-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="184"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="105"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR25423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="207" mass="23906" checksum="50E95387ACC5E87A" modified="2009-07-28" version="1">MLRGLNQTFRPKRMLHGSRMLLDSLDPVHIRNTNRFRQLLVEKGEFAPQQANALVEIMSDALKGGVTHVSQDLASREKLIQLVYQQRVDFAKLRDQLLSADRSEFHSIQNEHESIRNDLEKLRTKLREEITKANAGFKLDLSLEKGRIREESSHHDIQIKEIDTRIDQEVSNMKMQIDSVKTQVMQWLIGVCTGTFALVLAYVRLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNR9</accession>
    <name>C5DNR9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0G19316p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0G19316g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555867.1">
        <property type="nucleotide sequence ID" value="XM_002555821.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNR9"/>
    <dbReference type="FunCoup" id="C5DNR9">
        <property type="interactions" value="215"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNR9"/>
    <dbReference type="GeneID" id="8294162"/>
    <dbReference type="KEGG" id="lth:KLTH0G19316g"/>
    <dbReference type="eggNOG" id="ENOG502QVDZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_493537_0_0_1"/>
    <dbReference type="InParanoid" id="C5DNR9"/>
    <dbReference type="OMA" id="YWQYKAG"/>
    <dbReference type="OrthoDB" id="5598844at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013933">
        <property type="entry name" value="CRC_Rsc7/Swp82"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08624">
        <property type="entry name" value="CRC_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="309"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="318"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR25430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="518" mass="57789" checksum="1C5DBAFBC3D86DD9" modified="2009-07-28" version="1">MASKDTSRGTVNYFTEELAKEHELYLLKHALLIYNDPGLSLGPLSEPPNRSPMLPTYVIRERIADTGDCYVIARGDQQGEAKINRKGDLLGSRNFLFPTFTLPSRPHAVYVLVKDLIKCLESALSSAEFLHQNAQLYGVKASPEELEFLKENRLCSSEEANDDICFVTAKSAFMLFGARVVVGGTRLVDDYWEQITKEQGFLPHHRVFAIKSKILKVLLTLKPSATSDETSKILDCDIPNEPSYQIVSEQPSWELRQEYARDLTQGEHLSVIVPGQNITGSLELSAQFKLPKYHGKTSLQAAIQAGTQDTPIGALPSPFNTSQPAPSETASKSDKRLLSGILYPLAGPKLDKNSGTQSSGSSSSHDVTLNVNGWKFEMPVRSDPSDTCTYSSKGLPIHDSWSIVERLKSLTPNEINEMQHIHDSVYVNTGLQKARRTRKTRWTKYWQYKAGVPIGTTQQNASAVMQKYLKELETHIEVVRTLDEANDREQVTTIRRVPNPNFLGHSNITGLKPPYISE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>C5E1Y6</accession>
    <name>C5E1Y6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0H00682p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0H00682g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555909.1">
        <property type="nucleotide sequence ID" value="XM_002555863.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E1Y6"/>
    <dbReference type="FunCoup" id="C5E1Y6">
        <property type="interactions" value="64"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E1Y6"/>
    <dbReference type="GeneID" id="8294221"/>
    <dbReference type="KEGG" id="lth:KLTH0H00682g"/>
    <dbReference type="eggNOG" id="ENOG502RXUV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091043_0_0_1"/>
    <dbReference type="InParanoid" id="C5E1Y6"/>
    <dbReference type="OMA" id="EWDDIEM"/>
    <dbReference type="OrthoDB" id="4035012at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000446">
        <property type="term" value="C:nucleoplasmic THO complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018557">
        <property type="entry name" value="THO_cplx_su_Thp2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09432">
        <property type="entry name" value="THP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR30047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="258" mass="29178" checksum="DB1B1D86BCB1253C" modified="2009-07-28" version="1">MSSKTVERLLDDLHKSLDVNYAHSSELIESLSVLSSDSTDDKMIEAIRALQKTFHSFTTSLADLRDENINAMESHNTKRKVEPFANVAKSRLSATFDENGNASTLLSYVDEVITGNNLNLEHINLIGRLSSVLTEMLPSELDDSTQNLELAQILEAYNTDSKGSEDTDCLKEKLLDWIDSIKMEKARYSLENQHILRDSLKALTMEVTRWRENYESIEGMMFGENANSISQMLHKVQRLRPQLNSGSGEGQEEDVKME</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>C5E223</accession>
    <name>C5E223_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">ATP-dependent RNA helicase DHH1</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">ATP-dependent RNA helicase dhh1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="ordered locus">KLTH0H01562g</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">P-body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the DEAD box helicase family. DDX6/DHH1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555946.1">
        <property type="nucleotide sequence ID" value="XM_002555900.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E223"/>
    <dbReference type="FunCoup" id="C5E223">
        <property type="interactions" value="1448"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E223"/>
    <dbReference type="GeneID" id="8294259"/>
    <dbReference type="KEGG" id="lth:KLTH0H01562g"/>
    <dbReference type="eggNOG" id="KOG0326">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_30_0_1"/>
    <dbReference type="InParanoid" id="C5E223"/>
    <dbReference type="OMA" id="TYEDRHT"/>
    <dbReference type="OrthoDB" id="10265785at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17940">
        <property type="entry name" value="DEADc_DDX6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000114">
        <property type="entry name" value="ATP-dependent RNA helicase DDX6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000364">
        <property type="entry name" value="ATP-dependent RNA helicase DDX6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47960">
        <property type="entry name" value="DEAD-BOX ATP-DEPENDENT RNA HELICASE 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 17" id="KW-0347">Helicase</keyword>
    <keyword evidence="6 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="5 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="10" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="21">
        <location>
            <begin position="29"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="19">
        <location>
            <begin position="60"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="20">
        <location>
            <begin position="240"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="464"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="16">
        <location>
            <begin position="29"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="472"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00040210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00040448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="CAR30084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="491" mass="55533" checksum="2415D2DCB7744F4C" modified="2009-07-28" version="1">MEDDWKSQLNIPKKDTRPQTDDVLNTRGNTFEDFYLKRELLMGIFEAGFEKPSPIQEEAIPIAIAGRDVLARAKNGTGKTAAFVVPTLEKVKPKVNKIQALIMVPTRELALQTSQVVRTLGKHCGISCMVTTGGTNLRDDILRLNEPVHILVGTPGRVLDLASRKVADLSECPLFVMDEADKMLSRDFKAIIEQILTFLPPVHQSLLFSATFPLTVKEFMVKHLHKPYEINLMDELTLKGITQYYAFVEERQKLHCLNTLFSKLQINQAIIFCNSTNRVELLAKKITDLGYSCYYSHARMKQSERNKVFHEFRQGKVRTLVCSDLLTRGIDIQAVNVVINFDFPKTAETYLHRIGRSGRFGHLGLAINLINWNDRFNLYKIEQELGTEIAAIPAQIDKSLYVAEDDSAVPVPFPLASLPNTMANVAGQQQQLPPQLTQPQFTAVPQQYAPQQQQYMPQQQYQYPPQAFMQGPPQSMEQAPSMQGHAPQQTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>C5E224</accession>
    <name>C5E224_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">mitogen-activated protein kinase kinase</fullName>
            <ecNumber evidence="8">2.7.12.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">KLTH0H01584g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.12.2" evidence="8"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555947.1">
        <property type="nucleotide sequence ID" value="XM_002555901.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E224"/>
    <dbReference type="FunCoup" id="C5E224">
        <property type="interactions" value="579"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E224"/>
    <dbReference type="GeneID" id="8294260"/>
    <dbReference type="KEGG" id="lth:KLTH0H01584g"/>
    <dbReference type="eggNOG" id="KOG0581">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_63_23_1"/>
    <dbReference type="InParanoid" id="C5E224"/>
    <dbReference type="OMA" id="EFRHWCK"/>
    <dbReference type="OrthoDB" id="10252354at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051286">
        <property type="term" value="C:cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004708">
        <property type="term" value="F:MAP kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071507">
        <property type="term" value="P:pheromone response MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000040">
        <property type="entry name" value="Dual specificity mitogen-activated protein kinase kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000921">
        <property type="entry name" value="Serine/threonine-protein kinase STE7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48013:SF9">
        <property type="entry name" value="DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48013">
        <property type="entry name" value="DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="128"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="83"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="86"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00051693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAR30085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="462" mass="51072" checksum="1B7ABA414505A61C" modified="2009-07-28" version="1">MSFASQDVSLRQKSRPGESGLFQPSSLKRKNLKKLTLGGAVGTGRGLRTDNMVPVSITGSSGSNGIEAPLGLNGIPNGDLRGYGSSGVSPTSDPSSAGITPTEEVPGLTSDLENLSLFQDTTIELQDLVQLGKIGSGNSGTVLKTLHVPDSRIIAKKSIPVENKQLVKSQLMRELSIMRNVKPHDNIVGFFGAFYTASTSNEIVILMEYMDCGSLDKIMSTYKAFVARGIQSPTENWFSEPVLSKISFAVLNGLLYLYRGYKIIHRDIKPSNVLINSKGCVKICDFGVSKKLINSIADTFVGTSTYMSPERIQGSVYSTKGDVWSLGLMIIELVTGEFPLGGHSDTPEGILDLLQRIVNEPPPRLPPQLPNGLQYSREMFDFVNRCCVKDERERSSLQELLCHDFIEKSRSQGDREFRHWCKRIKKWLKEDKMLRREEQERAKLVKRQLESAANAATAARHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-04-02" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>C5E260</accession>
    <name>C5E260_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0H02376p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0H02376g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555983.1">
        <property type="nucleotide sequence ID" value="XM_002555937.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E260"/>
    <dbReference type="GeneID" id="8294296"/>
    <dbReference type="KEGG" id="lth:KLTH0H02376g"/>
    <dbReference type="eggNOG" id="ENOG502SFR5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1156576_0_0_1"/>
    <dbReference type="InParanoid" id="C5E260"/>
    <dbReference type="OMA" id="AIHGTHP"/>
    <dbReference type="OrthoDB" id="4062164at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="138"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR30121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="240" mass="26120" checksum="D41F6788553A699C" modified="2009-07-28" version="1">MSVLERPLSHNSPVFVPSPECTARAYTLPANTTGVRQLVIRKQNGRVVAAKSQLIDRMEDALVRWRPPASCHAGSTAALKGKHPYQQELDTDVDLTSVPLHASLDSIRHYFDNLGHTISRPCSQRPPKTEQLAGSTQSPASSPASSASGMAVQIRQSVQSAGTRGTTPRAMAYDADPDNIEARPASYSFPIPAYYLPHTDACPVGESTRENNEEAHGCWAQWTTFWDELCDDLAALCSCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>C5E262</accession>
    <name>C5E262_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0H02420p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0H02420g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555985.1">
        <property type="nucleotide sequence ID" value="XM_002555939.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E262"/>
    <dbReference type="FunCoup" id="C5E262">
        <property type="interactions" value="11"/>
    </dbReference>
    <dbReference type="GeneID" id="8294298"/>
    <dbReference type="KEGG" id="lth:KLTH0H02420g"/>
    <dbReference type="eggNOG" id="KOG4539">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043838_0_1_1"/>
    <dbReference type="InParanoid" id="C5E262"/>
    <dbReference type="OMA" id="PGFYLTY"/>
    <dbReference type="OrthoDB" id="5562676at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018786">
        <property type="entry name" value="Mit_KHE1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28062">
        <property type="entry name" value="K+-H+ EXCHANGE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28062:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10173">
        <property type="entry name" value="Mit_KHE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="181"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR30123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="298" mass="34821" checksum="559AE5EC7FB8AD55" modified="2009-07-28" version="1">MSTAGPSGLGKRLLSQGLPPQRATVQKYVLDPVKLIAIPITNKRIFLYHKHSSEILNMNSRIVRYETRITEKARKIWSKLEKSPREINQKLVRMVNSQLDKTPWTEDSLNTVPSEGYILKTVMENQESRNLTYQEWFKNVDKLDVRPVCMYYPQSVMSERAVREQLKSLWDHGLNYHKKQMWLSILGLPLTLPVVLVPVVPNVPGFYLLYRAYRNFKAFCGAKHLKSLVENKSHKLVFTDLPQFSDILRSETEIPEEHLVLNEKNLERVLDMLQIHELRAPLEKAVLQERKRLDGHRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2A0</accession>
    <name>C5E2A0_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">dolichyl-phosphate beta-glucosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.117</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0H03256g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a di-trans,poly-cis-dolichyl phosphate + UDP-alpha-D-glucose = a di-trans,poly-cis-dolichyl beta-D-glucosyl phosphate + UDP</text>
            <dbReference type="Rhea" id="RHEA:15401"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19502"/>
            <dbReference type="ChEBI" id="CHEBI:57525"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.4.1.117"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:15402"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.117" evidence="4"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556023.1">
        <property type="nucleotide sequence ID" value="XM_002555977.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2A0"/>
    <dbReference type="FunCoup" id="C5E2A0">
        <property type="interactions" value="688"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2A0"/>
    <dbReference type="GeneID" id="8294336"/>
    <dbReference type="KEGG" id="lth:KLTH0H03256g"/>
    <dbReference type="eggNOG" id="KOG2977">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033536_9_1_1"/>
    <dbReference type="InParanoid" id="C5E2A0"/>
    <dbReference type="OMA" id="HMVNTDA"/>
    <dbReference type="OrthoDB" id="3784at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004581">
        <property type="term" value="F:dolichyl-phosphate beta-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04188">
        <property type="entry name" value="DPG_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000169">
        <property type="entry name" value="Dolichyl-phosphate beta-glucosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035518">
        <property type="entry name" value="DPG_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10859:SF91">
        <property type="entry name" value="DOLICHYL-PHOSPHATE BETA-GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10859">
        <property type="entry name" value="GLYCOSYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="14">
        <location>
            <begin position="80"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR30161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="332" mass="37058" checksum="06180EE938D30C25" modified="2009-07-28" version="1">MWDTFWSRVRELSVSTVLVTFAVAIACSLYLIVLLLSHSPRKPFDEELIFKTNGPRGHIVSGKLDPVTKGSSNKDGIELSVVVPSYNEIGRILVGLTDAVTFLQEHLEDKWEILVVDDGSSDGTSKYCLDLAANHFKLKPGQLRVVKLTQNRGKGGAVRHGLLHMRGKYGLFADADGASRFSDVAKLLDSIKKHEEKGHAAMAIGSRAHMVNTDAVVKRSFIRNLLMYGLHTLVFIFGIRSIKDTQCGFKLFNRSATVQIFPYLHTEGWIFDVEILILALRKKVPVEEVAISWHEVDGSKMDLARDSINMAKDLVIIRMAYLLGIYSDKVEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2N4</accession>
    <name>C5E2N4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0H06380p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0H06380g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556157.1">
        <property type="nucleotide sequence ID" value="XM_002556111.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2N4"/>
    <dbReference type="FunCoup" id="C5E2N4">
        <property type="interactions" value="696"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2N4"/>
    <dbReference type="GeneID" id="8294472"/>
    <dbReference type="KEGG" id="lth:KLTH0H06380g"/>
    <dbReference type="eggNOG" id="KOG0745">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014218_8_2_1"/>
    <dbReference type="InParanoid" id="C5E2N4"/>
    <dbReference type="OMA" id="HRSDFTN"/>
    <dbReference type="OrthoDB" id="1721884at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050052">
        <property type="entry name" value="ATP-dep_Clp_protease_ClpX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019489">
        <property type="entry name" value="Clp_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003745">
        <property type="entry name" value="PRK05342.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48102:SF7">
        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE ATP-BINDING SUBUNIT CLPX-LIKE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48102">
        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE ATP-BINDING SUBUNIT CLPX-LIKE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07724">
        <property type="entry name" value="AAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10431">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01086">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="3">
        <location>
            <begin position="126"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="Clp ATPase C-terminal" evidence="4">
        <location>
            <begin position="360"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR30295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="508" mass="56650" checksum="A011C20022506860" modified="2009-07-28" version="1">MIKTGLKSFQSFHRLSSLAGKVQENQLRNLPSPKELRKFLDNYVVGQTTGKKVLSVAVYNHYLRVNDKQKRIDAKLQRELLEKQQQEDDKDEPIYTGSNEAKVGFQNLQTQLARGMDPKDDDLELSKSNVLVVGPSGSGKTLLASTLARVLDVPIAITDCTQLTQAGYIGEDVEVCIERLLINADYDVAKAERGIIVLDELDKLAKPAASIGTKDVSGEGVQQALLKILEGHSIEVTVKRPINKKKDGQNNNQVSAKKDETFVVDTSNILFMLMGAFVGLDKHVVKRINRLKAISNGETIEDKSEDSKRLRFSNTIEKVELSNGKKVSALNLATPTDLVAYGLIPELIGRVPIITALEPLERTDLYHILREPKNALLNQYEYIFKQFGVKLCVTEKALMKVAHFALDEGTGARGLRGIMERLLLNVNYECPGSGISYVLVNEDTVKSLQLSEHSLSTHVNAKYYSRGERDKFIEDVHKEDSKQGRELDRLYGRTATLVREGNVDQCPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2Q2</accession>
    <name>C5E2Q2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Acyl-CoA desaturase</fullName>
            <ecNumber evidence="16">1.14.19.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ordered locus">KLTH0H06798g</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Stearoyl-CoA desaturase that utilizes O(2) and electrons from reduced cytochrome b5 to introduce the first double bond into saturated fatty acyl-CoA substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>octadecanoyl-CoA + 2 Fe(II)-[cytochrome b5] + O2 + 2 H(+) = (9Z)-octadecenoyl-CoA + 2 Fe(III)-[cytochrome b5] + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:19721"/>
            <dbReference type="Rhea" id="RHEA-COMP:10438"/>
            <dbReference type="Rhea" id="RHEA-COMP:10439"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
            <dbReference type="ChEBI" id="CHEBI:57394"/>
            <dbReference type="EC" id="1.14.19.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="16">Expected to bind 2 Fe(2+) ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the fatty acid desaturase type 1 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.19.1" evidence="16"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556175.1">
        <property type="nucleotide sequence ID" value="XM_002556129.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2Q2"/>
    <dbReference type="FunCoup" id="C5E2Q2">
        <property type="interactions" value="995"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2Q2"/>
    <dbReference type="GeneID" id="8294491"/>
    <dbReference type="KEGG" id="lth:KLTH0H06798g"/>
    <dbReference type="eggNOG" id="KOG0537">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1600">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027359_3_2_1"/>
    <dbReference type="InParanoid" id="C5E2Q2"/>
    <dbReference type="OMA" id="WAHKSYN"/>
    <dbReference type="OrthoDB" id="10260134at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004768">
        <property type="term" value="F:stearoyl-CoA 9-desaturase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006636">
        <property type="term" value="P:unsaturated fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03505">
        <property type="entry name" value="Delta9-FADS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.120.10:FF:000004">
        <property type="entry name" value="Acyl-CoA desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009160">
        <property type="entry name" value="Acyl-CoA_deSatase_haem/ster-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015876">
        <property type="entry name" value="Acyl-CoA_DS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001522">
        <property type="entry name" value="FADS-1_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11351">
        <property type="entry name" value="ACYL-COA DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11351:SF31">
        <property type="entry name" value="DESATURASE 1, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000345">
        <property type="entry name" value="OLE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00075">
        <property type="entry name" value="FACDDSATRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00476">
        <property type="entry name" value="FATTY_ACID_DESATUR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 16" id="KW-0249">Electron transport</keyword>
    <keyword evidence="15 16" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="8 16" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5 16" id="KW-0349">Heme</keyword>
    <keyword evidence="12 16" id="KW-0408">Iron</keyword>
    <keyword evidence="4 16" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="13 16" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 16" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="89"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="119"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="234"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="18">
        <location>
            <begin position="387"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR000345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAR30313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="488" mass="55775" checksum="E5DAF1EDBC5B155A" modified="2009-07-28" version="1">MADTKADKMEQVDLVKANILAAGANKESVRVVNGLGSLMGSKNMVNVDFDKDSNMDYLLRKDQEEKDRYKNSKHISEQPWTIHNWHQHLNWLNMVLVVGIPLAGWYFALSGKVPLHMNVLLFSIAYYVLGGVSITAGYHRLWSHRAYSARWPLRLFFAIFGAASVEGSIKWWGHSHRIHHRYTDTVRDPYDARRGLWYSHMGWMLLQPNPKYRARADIADLTDDWIVRFQHRHYIPIMAFSAFILPALLCKYLFNDFIGGLVYGGVLRVFAIQQATFCINSLAHYLGDQPFDDRRTPRDNWITALVTFGEGYHNFHHEFPTDYRNAIKWYQYDPTKVFIFLSSLVGLSFNLKKFSQNAIQQGLVQQQQKKIDRQRSHLNWGTPLTQLPVWDKQQFMENIKTNPGLVIISGIVHDVSNYITEHPGGETLIQAALGKDATKAFNGGVYLHSNAAHNVLATMRVAVVKEGVDAAAKFAARRGESLEGKKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2R3</accession>
    <name>C5E2R3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glutathione hydrolase</fullName>
            <ecNumber evidence="8">2.3.2.2</ecNumber>
            <ecNumber evidence="8">3.4.19.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Gamma-glutamyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Gamma-glutamyltranspeptidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0H07062g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Cleaves the gamma-glutamyl peptide bond of glutathione and glutathione conjugates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 8">
            <text>an N-terminal (5-L-glutamyl)-[peptide] + an alpha-amino acid = 5-L-glutamyl amino acid + an N-terminal L-alpha-aminoacyl-[peptide]</text>
            <dbReference type="Rhea" id="RHEA:23904"/>
            <dbReference type="Rhea" id="RHEA-COMP:9780"/>
            <dbReference type="Rhea" id="RHEA-COMP:9795"/>
            <dbReference type="ChEBI" id="CHEBI:77644"/>
            <dbReference type="ChEBI" id="CHEBI:78597"/>
            <dbReference type="ChEBI" id="CHEBI:78599"/>
            <dbReference type="ChEBI" id="CHEBI:78608"/>
            <dbReference type="EC" id="2.3.2.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>an S-substituted glutathione + H2O = an S-substituted L-cysteinylglycine + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:59468"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="ChEBI" id="CHEBI:143103"/>
            <dbReference type="EC" id="3.4.19.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 8">
            <text>glutathione + H2O = L-cysteinylglycine + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:28807"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:61694"/>
            <dbReference type="EC" id="3.4.19.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 8">Sulfur metabolism; glutathione metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the gamma-glutamyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.2" evidence="8"/>
    <dbReference type="EC" id="3.4.19.13" evidence="8"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556186.1">
        <property type="nucleotide sequence ID" value="XM_002556140.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2R3"/>
    <dbReference type="FunCoup" id="C5E2R3">
        <property type="interactions" value="203"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2R3"/>
    <dbReference type="MEROPS" id="T03.012"/>
    <dbReference type="GeneID" id="8294503"/>
    <dbReference type="KEGG" id="lth:KLTH0H07062g"/>
    <dbReference type="eggNOG" id="KOG2410">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014813_4_0_1"/>
    <dbReference type="InParanoid" id="C5E2R3"/>
    <dbReference type="OMA" id="ICGMGPP"/>
    <dbReference type="OrthoDB" id="1081007at2759"/>
    <dbReference type="UniPathway" id="UPA00204"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036374">
        <property type="term" value="F:glutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103068">
        <property type="term" value="F:leukotriene C4 gamma-glutamyl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006751">
        <property type="term" value="P:glutathione catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.40:FF:000001">
        <property type="entry name" value="Gamma-glutamyltranspeptidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043138">
        <property type="entry name" value="GGT_lsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000101">
        <property type="entry name" value="GGT_peptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043137">
        <property type="entry name" value="GGT_ssub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00066">
        <property type="entry name" value="g_glut_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11686">
        <property type="entry name" value="GAMMA GLUTAMYL TRANSPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11686:SF9">
        <property type="entry name" value="RE13973P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01019">
        <property type="entry name" value="G_glu_transpept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01210">
        <property type="entry name" value="GGTRANSPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950724" description="Glutathione hydrolase" evidence="9">
        <location>
            <begin position="17"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="419"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="437"/>
            <end position="439"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="461"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="489"/>
            <end position="490"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="512"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR600101-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR600101-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU368068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR30324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="609" mass="67125" checksum="106EE7CF3FD1042D" modified="2009-07-28" version="1">MRLAAVLTLLLETAVCMQHFFVESPDHNIDIKPLPRHPTLSPNSSQVLRTGRYGAASSDSEHCNKIAVEQVLKKFDGANAADAAVSVALCLGMKNFFSSGIGGGGYAVFVGKDQMSGETDSLFIDFREQAPALAHKNMFSQSPNASQIGGLAIAVPGELRGIYELFVKKGSGKVEWRDLLQPIIELGSNGWEVDEITAACLKIYEPYFVQHKKDWAFVLNEGQSRVLKTGESIKRPALSDTLKELASNGTVAPFYDPNHRIGRSMLRKIKESGGIITGNDLKSYYVNTSKPLYTKIRSGWQYFPNNDLEVFTSGGSSSGAALLSALKILDEFPAKKGGDFQPEQVYVLVECMKWMSSARSRLGGFSDPHELPQRILEVLDSNWTQNSVRLINDHLPGLKTLSNWTEYNPAYALTDPHGTTHFGVVDKFGNAVSFTSTVNLLCGSLVHDIETGVIFNNQMDDFSQPGRSNAFELAPSIYNFIEPGKRPLSSASPVVILDELGKPDLVIGASGGSRITPSIFQVITRLYWYEMPLLEAIAYPRVHHQLLPDVLEVESFPMLGKDTLEALKSMGHNLLEQAPKSVVNGIKRHRGEWHAVSDYWRKRGLSVAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2W7</accession>
    <name>C5E2W7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">KLTH0H08294p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0H08294g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556240.1">
        <property type="nucleotide sequence ID" value="XM_002556194.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5E2W7">
        <property type="interactions" value="244"/>
    </dbReference>
    <dbReference type="GeneID" id="8294558"/>
    <dbReference type="KEGG" id="lth:KLTH0H08294g"/>
    <dbReference type="eggNOG" id="KOG3511">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000700_0_0_1"/>
    <dbReference type="InParanoid" id="C5E2W7"/>
    <dbReference type="OMA" id="ECDYNYY"/>
    <dbReference type="OrthoDB" id="443634at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.270:FF:000005">
        <property type="entry name" value="Sortilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031777">
        <property type="entry name" value="Sortilin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031778">
        <property type="entry name" value="Sortilin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006581">
        <property type="entry name" value="VPS10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050310">
        <property type="entry name" value="VPS10-sortilin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12106">
        <property type="entry name" value="SORTILIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12106:SF27">
        <property type="entry name" value="SORTILIN-RELATED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15902">
        <property type="entry name" value="Sortilin-Vps10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15901">
        <property type="entry name" value="Sortilin_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00602">
        <property type="entry name" value="VPS10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110296">
        <property type="entry name" value="Oligoxyloglucan reducing end-specific cellobiohydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950740" evidence="9">
        <location>
            <begin position="18"/>
            <end position="1536"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1358"/>
            <end position="1377"/>
        </location>
    </feature>
    <feature type="domain" description="VPS10" evidence="10">
        <location>
            <begin position="40"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="domain" description="VPS10" evidence="10">
        <location>
            <begin position="724"/>
            <end position="1345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1491"/>
            <end position="1536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1508"/>
            <end position="1527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR30378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1536" mass="170711" checksum="ACBFD33D4889B277" modified="2009-07-28" version="1">MIAFKLLLLVWASIALAKWEPRVTSTKSSLSFTLQVFDDSTTLMTIDHGKLKISHDNGLNWDVLDQLGRRVSRVEVDRNYPKSRAFAVDAEGKVVYKTDDKGQHWEKLNFDSPIVGDENDAPLSMTAYFETNPLSDGDILVHVAGCDIVDGNFLHCSSSKRVFVSSNGKDFSLVESLKAESEEKDGEEVFSVFCRFDGFKPEDSEAGDAHGIVCEVDSMETKDSETVRSSKVFQSSDYGKTLQIPHGFEDLNIVSLIHVGDYNVITTVEDKYNPDSASQLWISRDGLNYEKAVIPTRTGRYTFVLKFGSSSKELALQNNAFTDSGEKTPSVLISTSNGTKFTPLTEAAAVGYYSLSGVGNQEGVVAYTVSTPNYSDESKESPDKAKISFDNGKTWSNLRIDDPDHEFDCDTSDPENCYLRPLWFLEDWRNSHVTTTPGISACVAEVIGDSGKPSSFENVKTFITGNGGYSWHKILDFPASVVFGDYGNIILAVPFSPDNDGDPAAEFYYSLDQGNTWSEYQFKEPLYFINFVPTALDGSGATFILQAATPTGRDDGRFLTVDFSDAFDGNKCTDSDMGEWYTNGGGCVSGSKHKFRRRKEDAKCLVKELFKDLEPTEETCDCTKSDYECSPEFSLNKDGECVLDRNLLEISGTCGGENKDRKIKLLPMVLERGNKCRAPLKIDTIEIDCGAYFEGGEPSPIKIEEHTLDFGLKSYQYFDTIADETLLLGSDNHEVYISYDSGQTTQKLDFQDKIIEVVFNQYFNTSAYLFGSSNKLYVTHDRARSFKEYKLPDAKQLGFPLTFHAKDVDTFIFYGAKDCDSLFSPNCHAVAYITKDGGKRFRELTQGALRCEFIGSINDDSADPDMIMCLVKDKGANERHVVTSSNFFENQKTVFENAIGFISAGEYTIIAVPHDNDELRAYVTVDGREFAEASLPAELNIDKQQAYTVLGSQEGAIFFHLTTEMKQGSEYGALMKSNSNGTSFVVLERAVNRGSSGFVDFEKALGLEGIIMINTVSNAEEVSSGSGVQKKLKSKISFNDGADWIYLSPPERDSEGKKYQCDSKSLSSCSLNLHGYTERKDERDTYSSGSALGYMIGVGNVGEYLLPKEECSTFITTDGGFTWKEIKKGSYQWEFGDRGSVIVLVKDQQKTDLITYSVDSGKTWNDYKFAGEELYVEDIITTPQDSAMRFLLIGKSSSISGKDTKTFTVDFTQSFDRQCQLDRDYQYRVFGDCLFGHQAEYLQKVNDNCYNGAAPLEGMKKIVKNCSCTRSDFECDYNYYKANDGTCKLVKGLEPADPSQICKKDSDLIEYFEITGYRKIPLSTCEGGLRLDVASKSHACPGKEKEFKNKYGVSGRHILYIMIAPVLIFIGAAWFVYERGIKRNGGLSRFGEIRLGDDDLVEENFTDRVVNSVVRVGVFGFSALAASKQLVQRNAVNAWQRLKLRIQGGRGGPSYSSLTHEQLLDEADELLAGHDEDANDLASFIDSEGDYDIGAEELPQTHSPAPYTDHEPSDHETSSHETADHNNENGGNSNEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>C5E306</accession>
    <name>C5E306_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0H09306p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0H09306g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556279.1">
        <property type="nucleotide sequence ID" value="XM_002556233.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E306"/>
    <dbReference type="FunCoup" id="C5E306">
        <property type="interactions" value="79"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E306"/>
    <dbReference type="GeneID" id="8294604"/>
    <dbReference type="KEGG" id="lth:KLTH0H09306g"/>
    <dbReference type="eggNOG" id="ENOG502SCZQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_189322_0_0_1"/>
    <dbReference type="InParanoid" id="C5E306"/>
    <dbReference type="OMA" id="TAYFRYT"/>
    <dbReference type="OrthoDB" id="4069335at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050839">
        <property type="term" value="F:cell adhesion molecule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000752">
        <property type="term" value="P:agglutination involved in conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014404">
        <property type="entry name" value="Aga2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17366">
        <property type="entry name" value="AGA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950742" evidence="1">
        <location>
            <begin position="21"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR30417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="92" mass="9827" checksum="2DA7CF641C769C52" modified="2009-07-28" version="1">MKFSSVVTAYLAIFPSMCAAATVSWASVSCESISTGGTLETTPYSSSTATILKNGKEMVGVYEFFQSVTYISDCNPSTRKTSAEPNPTITIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>C5E343</accession>
    <name>C5E343_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Serine hydroxymethyltransferase</fullName>
            <ecNumber evidence="13">2.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0H10252g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 13">Interconversion of serine and glycine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O = (6S)-5,6,7,8-tetrahydrofolate + L-serine</text>
            <dbReference type="Rhea" id="RHEA:15481"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="EC" id="2.1.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 12 13">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5 13">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 13">Belongs to the SHMT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.1" evidence="13"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556316.1">
        <property type="nucleotide sequence ID" value="XM_002556270.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E343"/>
    <dbReference type="FunCoup" id="C5E343">
        <property type="interactions" value="469"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E343"/>
    <dbReference type="GeneID" id="8294647"/>
    <dbReference type="KEGG" id="lth:KLTH0H10252g"/>
    <dbReference type="eggNOG" id="KOG2467">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022477_0_0_1"/>
    <dbReference type="InParanoid" id="C5E343"/>
    <dbReference type="OMA" id="TQPFFSQ"/>
    <dbReference type="OrthoDB" id="10265628at2759"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004372">
        <property type="term" value="F:glycine hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019264">
        <property type="term" value="P:glycine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00378">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000097">
        <property type="entry name" value="Serine hydroxymethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00051">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001085">
        <property type="entry name" value="Ser_HO-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049943">
        <property type="entry name" value="Ser_HO-MeTrfase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019798">
        <property type="entry name" value="Ser_HO-MeTrfase_PLP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039429">
        <property type="entry name" value="SHMT-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000586">
        <property type="entry name" value="PRK00011.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680:SF57">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00464">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000412">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00096">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7 13" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="9 12 13" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Serine hydroxymethyltransferase-like" evidence="14">
        <location>
            <begin position="37"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="12">
        <location>
            <position position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00006376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000412-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR30454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="493" mass="54291" checksum="E7728F42D02C7615" modified="2009-07-28" version="1">MISRIASRQLKPVLVYRRLYATAKSTANQVMVSKHVQEIDPEMHEILTNERHRQKHSVTLIPSENFTSKSVMDLLGSEMQNKYSEGYPGERYYGGNQFIDQAESLCQKRALDLYGLDPEKWGVNVQPLSGAPANLYAYSAILDVNERLMGLDLPHGGHLSHGYQLPSGTKISYISKYFQTMPYHVNIQTGLIDYEMLAQTSKLFRPKVIVAGTSAYSRTLDYKRFKEITDACGAYLMSDMAHISGLVAAGVLPSPFEYSDIVTTTTHKSLRGPRGAMIFYRKGVRKVTKKGKEIMYDLDKRINFSVFPGHQGGPHNHTISALAVALKQAATPEFKEYQTAVVANASVFGEELVKRGFQLVSGGTDTHLVLIDLSNIGIDGARLETILEKINIAANKNTIPGDKSALFPSGLRVGTPAMTTRGFGPEDFAKVAEYIDKAAKLAIGLKSQESSEAKDARSKLANFKQLCAESDEVAALANKVSEWVGEFPVPGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3E7</accession>
    <name>C5E3E7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptide hydrolase</fullName>
            <ecNumber evidence="3">3.4.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0H12782g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M28 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.-.-" evidence="3"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556420.1">
        <property type="nucleotide sequence ID" value="XM_002556374.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3E7"/>
    <dbReference type="FunCoup" id="C5E3E7">
        <property type="interactions" value="171"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3E7"/>
    <dbReference type="GeneID" id="8294761"/>
    <dbReference type="KEGG" id="lth:KLTH0H12782g"/>
    <dbReference type="eggNOG" id="KOG3946">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045003_1_1_1"/>
    <dbReference type="InParanoid" id="C5E3E7"/>
    <dbReference type="OMA" id="TPFPSFW"/>
    <dbReference type="OrthoDB" id="3907302at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016603">
        <property type="term" value="F:glutaminyl-peptide cyclotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040234">
        <property type="entry name" value="QC/QCL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12283">
        <property type="entry name" value="GLUTAMINYL-PEPTIDE CYCLOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12283:SF6">
        <property type="entry name" value="GLUTAMINYL-PEPTIDE CYCLOTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005125188" description="Peptide hydrolase" evidence="3">
        <location>
            <begin position="20"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M28" evidence="4">
        <location>
            <begin position="94"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR30558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="324" mass="36774" checksum="91C3D773F772546D" modified="2009-07-28" version="1">MKSLRAFLVVLTLIQASLGILFGKEFYDNSLDRKLSLDPSTSESLLIPFNITRVPGSSGSEKVQEFITKHFKTLQSDWLIEPLQFAENSINFTNLVFTLGKNASSYLMLAAHYDSKKSPAGFIGATDSAASCAILMYISEFLDSLETGINAGLEESFWQGSKGLKIVFFDGEEALNQWTSEDSIYGARNLASLWKRDGTIEKIEAMILLDLLGSKDHVTVPSYFEQSDMMYKKIVEIKTQNTDALPFKEELLDVSERKFVEAKGNYIEDDHTPFWQHGIPVLHLIPIPFPSYWHTIEDNFSNLDEVKIKTWAILLCEFLYDRFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3N9</accession>
    <name>C5E3N9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0H15136p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0H15136g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556512.1">
        <property type="nucleotide sequence ID" value="XM_002556466.1"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3N9"/>
    <dbReference type="GeneID" id="8294877"/>
    <dbReference type="KEGG" id="lth:KLTH0H15136g"/>
    <dbReference type="eggNOG" id="KOG0783">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1426">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005054_0_0_1"/>
    <dbReference type="InParanoid" id="C5E3N9"/>
    <dbReference type="OMA" id="FEFVLRY"/>
    <dbReference type="OrthoDB" id="1893551at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051625">
        <property type="entry name" value="Signaling_Regulatory_Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22872">
        <property type="entry name" value="BTK-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22872:SF2">
        <property type="entry name" value="INHIBITOR OF BRUTON TYROSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13540">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005668290" evidence="4">
        <location>
            <begin position="20"/>
            <end position="1486"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="297"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="349"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="5">
        <location>
            <begin position="922"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1144"/>
            <end position="1192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1334"/>
            <end position="1417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1440"/>
            <end position="1486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1145"/>
            <end position="1157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1168"/>
            <end position="1188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1356"/>
            <end position="1376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1440"/>
            <end position="1450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="1461"/>
            <end position="1471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR30650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1486" mass="167585" checksum="F1E22972D3062F78" modified="2009-07-28" version="1">MGSICVSCVYCFMISALHCCSVVSNENTDAGKRTLIIITAHVQSTRPSRNFTEAFRPYDALTYFNRPKIHSSSSSSMNGKKQTESHDVFGRDLSYLLSCHPGIEQLNKVDLYSREPESGYTPLHVCLRLGHLEKAFKLYERWKRQQSHFRASKENIWDLKDREGLTPIELYQCENDIWNYQNIPTAIYPERPTTGPSGGVSSIVQWKPRSRDGNASPLRRHTIEELINTYRKYRGGREFLTTGSNTHLQLGTGDSENRQELYKLNEYSLYPEDTCQKTRFSTAIMKRYHSLLVTAEGDVFTAGSGSRGRLGNGDTNASNVCHTKVSLDSERVKLIDSSDHHSIVLTTSGEIFCWGWNRYSQLGYSTPTSNVKKLDNSALENICSPKPKRVVSAPWKKNMSISVKFVACSKVHSCLIDDNNYLFSWGLNLGQMGTAKNYGENYDALHAGHKGWIVSPPCGTKLPAYVTNIRQLLCTEFATFILFEDNQLCVLNNSKILRFSIPKAMSKQQLSSNGFDIFVPTSLSKNNKVLKLKTAHSYGNNMCFLYGNGSVGILQSELINNSAHSWSKLPNLLPTTQYWVPYFSWNKCLDFDVGAKGQLALCTVSGKLFKCETLSGQKFHRQRSNKLISGKCILVSCDSLFSSFALIKDDIDMIPLGFPKQNLYKDIAAISPLSKWPIERFRRLKCEELRDKPDSGFLFDDFAKPGARVEHSDKESVILDVGNSLSTNNQADSLWRTFSSRWFKKTSFVQSEIFSNESNRNYSLFNSEGAHDRYDVLFVDGKTGQIVGACHRALLMARAPNFIKQITTFGSFTPKSNHSLSFVLKSDLCDNKGCTVIQMVSGNIDLRAICCALHYMYTDEIPELGFISETQERKHFEASAKQFMKALDLHLTTFRGDKLPFALAKLLKQSNAEYDLSGEFSPDVIIELTNGEQFHAHSFILRNRSAFFESALSNRWAKLTGNIKVLKMHQVSKDELFCIMKYIYGFGFEELFSQCEFGGYKEFVNFVLGLIQTSDEFMMTGLKHYLESILIDYIDSSTVMIILVNAHRLCSKALVIECCWFIQHNIGLLFCEGNFKIINEYFDSYLWRTIQDFFSRTRSMNSINFPTWYNENGNPSYTIRLYESNIKKFNELFMDPDNAFEPSFELNTQQKKPTTLRKNSDRRKSSSARKMSLSQDDLANVRRPSSSAGDVKKQGFNLNLSFMAKNSEEAIEDDSVIIGNGGFVTVAKSKRKPSKSSPATKSLEVSASKTVSNSANVNVAMFENVTRLDSQEARSQERALSRASMFPVLANAVLSTTSSEEISKVAPSSTSIPFSTAKKISQKERLKTITAENKQTPVAQKKPVWGGKSSKAIPNPASTTVTPSASKFPSLSQSLHHQVPKKKAPSKGLTSTGESCTIPLYLSNPNTNTVKPPRSLKETIEEERFAKWWAEESERVQQELKKQEAEEREALNNIYSSGQPSRKKNVKKQKPQRPVNRKQAQAKTST</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3R5</accession>
    <name>C5E3R5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0H15730p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0H15730g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556538.1">
        <property type="nucleotide sequence ID" value="XM_002556492.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3R5"/>
    <dbReference type="FunCoup" id="C5E3R5">
        <property type="interactions" value="258"/>
    </dbReference>
    <dbReference type="GeneID" id="8294904"/>
    <dbReference type="KEGG" id="lth:KLTH0H15730g"/>
    <dbReference type="eggNOG" id="KOG4488">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165034_4_1_1"/>
    <dbReference type="InParanoid" id="C5E3R5"/>
    <dbReference type="OMA" id="HAKKQTE"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007513">
        <property type="entry name" value="SERF-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04419">
        <property type="entry name" value="SERF-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Small EDRK-rich factor-like N-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR30676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="62" mass="7005" checksum="5CD5BD323FB0D420" modified="2009-07-28" version="1">MSRGNQRELARQKNLSKQKQQAKGKKEGDPKKRMAADADILRQKQAAADARKAEQNQSGQRK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-06-28" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3S4</accession>
    <name>YIM11_LACTC</name>
    <protein>
        <recommendedName>
            <fullName>Protein YIM1-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">YIM1-1</name>
        <name type="ordered locus">KLTH0H15928g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lipid droplet</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the YIM1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3S4"/>
    <dbReference type="SMR" id="C5E3S4"/>
    <dbReference type="FunCoup" id="C5E3S4">
        <property type="interactions" value="166"/>
    </dbReference>
    <dbReference type="KEGG" id="lth:KLTH0H15928g"/>
    <dbReference type="eggNOG" id="KOG1198">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_3_3_1"/>
    <dbReference type="InParanoid" id="C5E3S4"/>
    <dbReference type="OMA" id="IDWKLQD"/>
    <dbReference type="OrthoDB" id="3509362at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08247">
        <property type="entry name" value="AST1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050700">
        <property type="entry name" value="YIM1/Zinc_Alcohol_DH_Fams"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11695">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11695:SF294">
        <property type="entry name" value="RETICULON-4-INTERACTING PROTEIN 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13602">
        <property type="entry name" value="ADH_zinc_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0551">Lipid droplet</keyword>
    <keyword id="KW-0496">Mitochondrion</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000409677" description="Protein YIM1-1">
        <location>
            <begin position="1"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="360" mass="39962" checksum="119FE787F0383103" modified="2009-07-28" version="1">MSESVANRAVVFYNNKSLPIISSEELNLDSCYSSSEVVIRVHAAALNPVDFLLQSFAYSWLVGRGPKTFSRDYSGEVVRAGANVKDFKVGDKVSGLFQHLYGKQGTLCDYLILDPVKQPAISKLAAAGHAEYDDYVVNAAWPLVFGTAYQGLTNFGQKLGPDSKILVIGASTSVSNAFVQIAKNHLKVGTVVGICSKKSFDYNKKLGYDYLAAYDDGSVVDSVKDIIGKNLNNEKFDLIFDSVGNSDFFGCINDVLKDKGQNSQFVSLTGDKKMNYSSPRIWDSLPGLESLKRYGPFRKYNYNLFMLRSDSAFMKLGYEMISEKQYMPAIDSVYSFDDYAKAFERVKSNRSKGKVVIKIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-04-02" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3T8</accession>
    <name>C5E3T8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0H16236p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0H16236g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556561.1">
        <property type="nucleotide sequence ID" value="XM_002556515.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3T8"/>
    <dbReference type="GeneID" id="8294927"/>
    <dbReference type="KEGG" id="lth:KLTH0H16236g"/>
    <dbReference type="eggNOG" id="ENOG502SFX5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035753_0_0_1"/>
    <dbReference type="InParanoid" id="C5E3T8"/>
    <dbReference type="OMA" id="NWGEIAL"/>
    <dbReference type="OrthoDB" id="435881at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050613">
        <property type="entry name" value="Sec_Metabolite_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001">
        <property type="entry name" value="UNCHARACTERIZED TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="3">
        <location>
            <begin position="15"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR30699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="563" mass="63336" checksum="601DCDC99C5D89F3" modified="2009-07-28" version="1">MSLPARDAQGISIISCQQCRERKVRCSRHIPRCRSCDKREYVCRYPQTHRKTSTKLTKKRRSENTRFYGFSSVNKALFEVGMPFCNIDSELESKNAPSQMRRFMSSSVYDRCIKNPEIAVDAVRSIKDSIGCFLFSEAMNLTGLEETLLSNGNIDYQCLLLLYSAIVFSERFEKFSPEVTSVVNELNTVLDDCPDCPEKVVSLILLADYYHYKLKIETAWKCTFLATSIAYALGLHLSSSKVWSMLVLHDALLCSVVGRPSSIKWVNPKLVGRACNNWGEIALLLREANDILLVSYNWTIEKVISLDFRFNDLIERVKKDMKVSSAVNDARDTLSWYLKLCILTASRIKLIYPVCSRYEALKPQLDQNCSDLSQCLRGIFGLLTSSKLATKENPYYLRSQCFLSYCSIFQGLLLELHFCSTQVTSGSAGSLEKENNFPLPQGTSSAVSQPCSLTNISVLLQEYDRVSDSVKLCSFMLDVFTSFRVLISKKEGKGAYEESFNTSVSPIGLTEESISNCSSFSESPINVEPSGNSLSWITGDMADWITACFSGEPPTFYAPENLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-02-15" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>Q5K457</accession>
    <name>Q5K457_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small ribosomal subunit protein uS3m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Ribosomal protein VAR1, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">var1</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <geneLocation type="mitochondrion" evidence="8"/>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2005" name="FEBS Lett." volume="579" first="30" last="40">
            <title>The complete mitochondrial genome of the yeast Kluyveromyces thermotolerans.</title>
            <authorList>
                <person name="Talla E."/>
                <person name="Anthouard V."/>
                <person name="Bouchier C."/>
                <person name="Frangeul L."/>
                <person name="Dujon B."/>
            </authorList>
            <dbReference type="PubMed" id="15620687"/>
            <dbReference type="DOI" id="10.1016/j.febslet.2004.10.106"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uS3 family.</text>
    </comment>
    <dbReference type="EMBL" id="AJ634268">
        <property type="protein sequence ID" value="CAG25607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_184729.1">
        <property type="nucleotide sequence ID" value="NC_006626.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q5K457"/>
    <dbReference type="FunCoup" id="Q5K457">
        <property type="interactions" value="91"/>
    </dbReference>
    <dbReference type="STRING" id="559295.Q5K457"/>
    <dbReference type="GeneID" id="3238984"/>
    <dbReference type="InParanoid" id="Q5K457"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Mitochondrion"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007980">
        <property type="entry name" value="Ribosomal_uS3m_fun"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05316">
        <property type="entry name" value="VAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 8" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAG25607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="373" mass="43521" checksum="C57FA32685B51998" modified="2005-02-15" version="1">MNLNLLKILLNNNSSNISGELNKDMKKWLLKNILLELNKNRLNKGLNNKEVNKYMNELNNKGNKLQHLNTMNIWSTQIYNYNKNLEINTTILDKLVTKLLYKLTTLKLLKNNNVILKNILISKPIFEHTTNRVNIKFYYYYKSSGINNTNLNKINNYYLSIISKLMNLLNNDNNNLSKILSYYYNKKITIEPIKLSYTYLNSDIFSQSINKDVNKYNNGIKGQYSRLLNSNIPNLNDQIIAKNYINNINNINNIKYNNIINNLNNSSTHYIKSGVSNIYNSLNINNIPMNILMFKYLTGWSITLNGRLASSKAISRTNTYRVLVGTFRNKKYLWGHINNNYKLNYIPANHNISNLSNVNKNGKYNIKVKLNFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-02-15" modified="2025-06-18" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q5K466</accession>
    <name>Q5K466_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cytochrome c oxidase subunit 1</fullName>
            <ecNumber evidence="15">7.1.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">cox1</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <geneLocation type="mitochondrion" evidence="18"/>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2005" name="FEBS Lett." volume="579" first="30" last="40">
            <title>The complete mitochondrial genome of the yeast Kluyveromyces thermotolerans.</title>
            <authorList>
                <person name="Talla E."/>
                <person name="Anthouard V."/>
                <person name="Bouchier C."/>
                <person name="Frangeul L."/>
                <person name="Dujon B."/>
            </authorList>
            <dbReference type="PubMed" id="15620687"/>
            <dbReference type="DOI" id="10.1016/j.febslet.2004.10.106"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>4 Fe(II)-[cytochrome c] + O2 + 8 H(+)(in) = 4 Fe(III)-[cytochrome c] + 2 H2O + 4 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:11436"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="7.1.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 15">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Mitochondrion inner membrane</location>
            <topology evidence="15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the heme-copper respiratory oxidase family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.9" evidence="15"/>
    <dbReference type="EMBL" id="AJ634268">
        <property type="protein sequence ID" value="CAG25598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_184720.1">
        <property type="nucleotide sequence ID" value="NC_006626.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q5K466"/>
    <dbReference type="FunCoup" id="Q5K466">
        <property type="interactions" value="620"/>
    </dbReference>
    <dbReference type="STRING" id="559295.Q5K466"/>
    <dbReference type="GeneID" id="3239004"/>
    <dbReference type="InParanoid" id="Q5K466"/>
    <dbReference type="UniPathway" id="UPA00705"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Mitochondrion"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015990">
        <property type="term" value="P:electron transport coupled proton transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01663">
        <property type="entry name" value="Cyt_c_Oxidase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.210.10:FF:000001">
        <property type="entry name" value="Cytochrome c oxidase subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.210.10">
        <property type="entry name" value="Cytochrome c oxidase-like, subunit I domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023616">
        <property type="entry name" value="Cyt_c_oxase-like_su1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036927">
        <property type="entry name" value="Cyt_c_oxase-like_su1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000883">
        <property type="entry name" value="Cyt_C_Oxase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023615">
        <property type="entry name" value="Cyt_c_Oxase_su1_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033944">
        <property type="entry name" value="Cyt_c_oxase_su1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10422">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10422:SF18">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00115">
        <property type="entry name" value="COX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01165">
        <property type="entry name" value="CYCOXIDASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81442">
        <property type="entry name" value="Cytochrome c oxidase subunit I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50855">
        <property type="entry name" value="COX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00077">
        <property type="entry name" value="COX1_CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 15" id="KW-0186">Copper</keyword>
    <keyword evidence="10 15" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5 15" id="KW-0349">Heme</keyword>
    <keyword evidence="12 15" id="KW-0408">Iron</keyword>
    <keyword evidence="14 15 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15 18" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="15" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="7 15 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 15" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="16"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="58"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="105"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="147"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="184"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="244"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="274"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="303"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="338"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="371"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="414"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="452"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome oxidase subunit I profile" evidence="17">
        <location>
            <begin position="1"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAG25598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="534" mass="58669" checksum="1E2D224FBC1B2401" modified="2005-02-15" version="1">MVQRWLYSTNAKDIAILYFIFAIFCGMAGTAMSVIIRLELAAPGNQYLGGNHQLFNVLVVGHAVLMIFFLVMPALIGGFGNYLLPLMIGASDMSFARLNNISFWLLPPALVCLVTSTLVESGAGTGWTVYPPLSSIQAHSGPSVDLAIFALHLTSISSLLGAINFIVTTLNMRTNGMTMHKLPLFVWAVFITAFLLLLSLPVLSAGVTMILLDRNFNTSFFEVAGGGDPVLYQHLFWFFGHPEVYILIIPGFGIVSHIVSTYSKKPVFGEISMVYAMASIGLLGFLVWSHHMYIVGLDADTRAYFTSATMIIAIPTGIKIFSWLATIYGGSIRLATPMLFAIAFLFLFTVGGLTGVALANASLDVAFHDTYYVVAHFHYVLSMGAIFSLFAGYYYWSPQILGLYYNEKLAQIQFWLIFVGANTIFLPMHFLGINGMPRRIPDYPDAFAGWNYVASVGSIIATVSLFFFIYIVYDQLVNGLDNKATNKSVLYAKSPDFIESNEIFALNTVKSSSIEFLLTSPPAVHSYNTPAVQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBC9</accession>
    <name>C5DBC9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0A01496p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0A01496g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551528.1">
        <property type="nucleotide sequence ID" value="XM_002551482.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBC9"/>
    <dbReference type="FunCoup" id="C5DBC9">
        <property type="interactions" value="61"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBC9"/>
    <dbReference type="GeneID" id="8290320"/>
    <dbReference type="KEGG" id="lth:KLTH0A01496g"/>
    <dbReference type="eggNOG" id="ENOG502QTEW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018731_1_0_1"/>
    <dbReference type="InParanoid" id="C5DBC9"/>
    <dbReference type="OMA" id="YEILPCI"/>
    <dbReference type="OrthoDB" id="5405745at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024338">
        <property type="entry name" value="MID1/Yam8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39142:SF1">
        <property type="entry name" value="AEL197CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39142">
        <property type="entry name" value="MID1P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12929">
        <property type="entry name" value="Mid1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002948647" evidence="1">
        <location>
            <begin position="25"/>
            <end position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="528" mass="59025" checksum="1C417BA2C07946AF" modified="2009-07-28" version="1">MTNEMYWGLWAITWIAAFLGTVQGYRDFSVSDEEVQRASLLNSNLIQEWTPIESVITAGKRDYFTFQINSTYAHTMSKLDMLVFISGNLHSESARLSNSTELQLAVWYSFDQEVTSDTSKGSRALFSAGYMEALAATPLEDQDGNLTEYSNLYLVIEAYNVTSNSPLQLTSTTQDLSWNYTIGVSQYDLVFQWDVRSWVELVDSDADSALLVTGNVTSLTTTSANSSLYDVGLYELYLYSYDELIAFDKALPHSLRAVQDGPYLASSVNNSAVKIELSNSSTPEIVKSLTVRGGSVKEQFYITGLNHSTTYGVYLTKKISDNTEVLSDSGGILFSKATFTTMADDSCSLIFGLDFCDGVAYSVPTAKSAGTNKTVTSLIYDGIASSLYSNFSKALQMIPCDTEKDARFSPLRTCNDCANSYRNWLCAVTIPRCTTENSSYYIHRDKTQNRNDFLNQQIQPIHDYYEILPCIDMCYNLAADCPPDFQFSCPSKTSHSELLYLSYSVWEENDFHTCNYVGNSRNLHILNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBL3</accession>
    <name>C5DBL3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Casein kinase II subunit alpha</fullName>
            <shortName evidence="11">CK II alpha</shortName>
            <ecNumber evidence="11">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0A03542g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine. Phosphorylates YTA7 during S-phase to promote transcription of histones.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Heterotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="11"/>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551612.1">
        <property type="nucleotide sequence ID" value="XM_002551566.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBL3"/>
    <dbReference type="FunCoup" id="C5DBL3">
        <property type="interactions" value="1195"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBL3"/>
    <dbReference type="GeneID" id="8290412"/>
    <dbReference type="KEGG" id="lth:KLTH0A03542g"/>
    <dbReference type="eggNOG" id="KOG0668">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_70_4_1"/>
    <dbReference type="InParanoid" id="C5DBL3"/>
    <dbReference type="OMA" id="YHDVNTH"/>
    <dbReference type="OrthoDB" id="10254671at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032545">
        <property type="term" value="C:CURI complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005956">
        <property type="term" value="C:protein kinase CK2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034456">
        <property type="term" value="C:UTP-C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007535">
        <property type="term" value="P:donor selection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042790">
        <property type="term" value="P:nucleolar large rRNA transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060962">
        <property type="term" value="P:regulation of ribosomal protein gene transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006356">
        <property type="term" value="P:regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006359">
        <property type="term" value="P:regulation of transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14132">
        <property type="entry name" value="STKc_CK2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000459">
        <property type="entry name" value="Casein kinase II subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000088">
        <property type="entry name" value="Casein kinase II subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045216">
        <property type="entry name" value="CK2_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24054">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24054:SF0">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="40"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00053883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU369118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR21170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="378" mass="44847" checksum="5F434EDB38C20713" modified="2009-07-28" version="1">MKCRTWSEARVYTTANVRRGQAYWDYENTVIDWVPNNGTYEIETKVGRGKYSEVFQGVQLSTKSKIVIKMLKPVKKKKIKREISILTNLSNETNPPTAQPFNKDAYYENKKEEVLQFTRSEVYELAHDGHKNIIQLLDVVKDQVSRTPALIFEHVDNVDFRTLYPTFTDLDVRFYMFELLKALDYCHSMGIMHRDVKPHNVMIDHKRKKLRLIDWGLAEFYHPNMEYNVRVASRFFKGPELLVDYRMYDYSLDLWSFGTMLASMIFKKEPFFHGTSNTDQLVKIVRILGSDDFENYLNKYQITLPQEFHDIDQYIRRPWHRFVNDSNKHLSGNGDIIDLIDNLLRYDHQERLTAREAMQHSWFAPIRRGEYNGEPDAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBV0</accession>
    <name>C5DBV0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0A05544p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0A05544g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551699.1">
        <property type="nucleotide sequence ID" value="XM_002551653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBV0"/>
    <dbReference type="FunCoup" id="C5DBV0">
        <property type="interactions" value="155"/>
    </dbReference>
    <dbReference type="GeneID" id="8290503"/>
    <dbReference type="KEGG" id="lth:KLTH0A05544g"/>
    <dbReference type="eggNOG" id="ENOG502RIY9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025801_0_0_1"/>
    <dbReference type="InParanoid" id="C5DBV0"/>
    <dbReference type="OMA" id="CFAYANE"/>
    <dbReference type="OrthoDB" id="4067584at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031852">
        <property type="entry name" value="Vik1/Cik1_MT-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16796">
        <property type="entry name" value="Microtub_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Spindle pole body-associated protein Vik1/Cik1 microtubule binding" evidence="3">
        <location>
            <begin position="350"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="124"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="252"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR21257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="631" mass="73162" checksum="9DC1F2C5645E0F73" modified="2009-07-28" version="1">MTHTKIPQMSQTRIPGHKRAMSSPPGSATKRLHILEDVTNTNSIARLPGTKMSVAVDSNTRLMNKYYYGDPKVIEAVKVRERKIARDIQHFRKSIAEIDAETKLIQETNLPNLDYDMSKKNTLCNELRKELVQLTTSLDEKNGECELMESNWELTVKHLQLEHEVNLQKARNEIDQALCEARERWEEKLREMENFRPDSGIIEEIEELKEERLQEEKKLNALEASNKRACTMRDQELDIEFQKFLLEKQKPSEELREKCQVQREKNKQLTEEILCFENQLEESTRDCARLYEAITRIEKLIKACLEEREELLERRTKVEASFRETELTTQKVQTEASAVESKYNAQFDKMEKEQLRRRKMENSIDEMRAKVRCFAYIGDKVPPSCEIDYPAKKISMIGTNSGQSSVFSRIIPRKLIPEKDLIGQECQAYLDMCTAKGLNCNIISLPTDRQQHLRECFLQYFVNSNTHKLSVQYVALSEKMPSSDLLVDPEDSKGDELQLTIKEDAIEMSSRSVELESPQDITDAFSGLELHSRVGDISVMKLKVYNKSEDYFDAYFLEINDDQTIKNISNLSNKNRFVKTPITIILQTLLLKTKSLILCNLFDGRTSDPCLVLESVHQIGRLDVPRKLNER</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-04-02" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBX1</accession>
    <name>C5DBX1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0A06006p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0A06006g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551720.1">
        <property type="nucleotide sequence ID" value="XM_002551674.1"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBX1"/>
    <dbReference type="GeneID" id="8290524"/>
    <dbReference type="KEGG" id="lth:KLTH0A06006g"/>
    <dbReference type="eggNOG" id="ENOG502QZ1C">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004695_0_0_1"/>
    <dbReference type="InParanoid" id="C5DBX1"/>
    <dbReference type="OMA" id="GRSHIEY"/>
    <dbReference type="OrthoDB" id="3362494at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037508">
        <property type="entry name" value="Msb1/Mug8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012965">
        <property type="entry name" value="Msb1/Mug8_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28093">
        <property type="entry name" value="MORPHOGENESIS-RELATED PROTEIN MSB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28093:SF1">
        <property type="entry name" value="MORPHOGENESIS-RELATED PROTEIN MSB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08101">
        <property type="entry name" value="Msb1-Mug8_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Meiotically up-regulated protein Msb1/Mug8" evidence="2">
        <location>
            <begin position="169"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="12"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="587"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="699"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="992"/>
            <end position="1171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1183"/>
            <end position="1261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1321"/>
            <end position="1397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="617"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="637"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="702"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="719"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="817"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="855"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="897"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="952"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1001"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1056"/>
            <end position="1069"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1071"/>
            <end position="1086"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1131"/>
            <end position="1148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1149"/>
            <end position="1164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1222"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1342"/>
            <end position="1360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR21278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1449" mass="160631" checksum="7689CE4BC56DE61D" modified="2009-07-28" version="1">MIKIRAFTKKLSGGFDSPKAHKKRSVSEYDKKHESSRSARSSMRRRAISSTQNIKTELPPLPTQEYTLSTASTASQDDTLGSPARASRSGKHYIYDQEAYKKASSALAKPDPNYSILNKKNGINPTSAKDVMHYLVLHFKHTLIQQTTGESDKKMDSIKAAMEIFRPNLSHYVSHEVGETIHILESFFPLDGCSLTGDALKKEINEQIGTSKHKLILALRIIWSKLPLGVVPWESYLKFCTVESKQNFSKMCFENFLPQVLPDNDYKCCAFDFMDVLVSIISMVDLVVDKAAQMDLLFTAGQVCFVKTPELLNYIDHKDSENADCITLAKLYRARGEALYRLFVSYLNSLAEEGKIKNFYLIDNFRIDEYPPKPYRPVTQRALTLTIPQLWDPEENNFNELIRVAAKTQIRTYSSNHPFSKLENSFLDKFEENPYKIVSTLFSRSSKRYLYKFDPHFDAEYFKPLSKKYGTPSTLGPGDQQAVATWINSCKERGFNDFLSVLDDNNHGEGTLALGFSFPKVSQEESESLPPVRVSKVEISECFISSWKYETFLAKVHNTLVIKLTKRIGDCEWLVIAMDERVGNFGYVAPSKPNESAKERDWSTLSTPPLPSKNPASYHSASDSMSSVKTRPPPPSLLGEPSSSPLLDSATGVFSDRSSVRLSSRPTSDLTSVYSNVRHHTKNNSSFDQRKFSAFDVHESPLQHSSPRLSSQSEHVTKVGSHSSKVSSRVISVSSPLSEVRDDEETQPLRTEKKEATETPSVQHKSHSNKNDANESQLQQPKTGAEAAAKPEISRGLESPLKLDRVPASQGTAKVENSARVEEPLKSEQTEKVGQHAEAEKCAEAKTPVNAENPVKVEEPLKAEESLKFEKPEETEEPEKTEKPEKTGEALKANKPLKTEEPLKAEEPVKAKEVVENEEPVKATGDEKFVKAEEPSKLEKPYEVGRSAEINESVKTEEQPKIEEHAKAEEPVRGEELLKAKEFSKVKQLAQLDDEPKVETTIKSNDINENTVPLCGQSPTEKEETVQSSSPDENVEAKNALAESAANRTLHAEENYSLSKNGKNLTQLRLSKGSTDSDSSGETSSDQEISSLESAVEVPEVHGQKPGFGHSSETLTIGDNLAKNERKQKGLSESTQTTIDWAQQSEPSDSAKKPESESKADSKAEVTLQSPLAIKLKYEDSSLSVSTPVNALPPNGLPQLPMKVQNPSVASFADATVKPDSAESTMDTSETTQVQEEPKAKAKAATKNPEPYSGSIQKRNSRVVEELRKSKILSNDVLTEDLLSDLLENYDTLSTEPINSENGPTVFERVRVYFERQAANAEEAFSGSTDGDSPATRESHTESLNSKNGTWFSCNVTPPNRSGKRVKNPKNLQLHGTERAAGGPDGSGALSRTPSVAGRGLEKNGVWLELEDHPSSPAEMSSPTQAMFRHMMMKTKRSLRSLKKSSDAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBX6</accession>
    <name>C5DBX6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0A06138p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0A06138g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551725.1">
        <property type="nucleotide sequence ID" value="XM_002551679.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBX6"/>
    <dbReference type="FunCoup" id="C5DBX6">
        <property type="interactions" value="94"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBX6"/>
    <dbReference type="GeneID" id="8290530"/>
    <dbReference type="KEGG" id="lth:KLTH0A06138g"/>
    <dbReference type="eggNOG" id="ENOG502QT50">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079101_3_0_1"/>
    <dbReference type="InParanoid" id="C5DBX6"/>
    <dbReference type="OMA" id="GDSRWQT"/>
    <dbReference type="OrthoDB" id="1915122at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007667">
        <property type="entry name" value="Hypoxia_induced_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040153">
        <property type="entry name" value="Rcf2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28018">
        <property type="entry name" value="RESPIRATORY SUPERCOMPLEX FACTOR 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28018:SF3">
        <property type="entry name" value="RESPIRATORY SUPERCOMPLEX FACTOR 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04588">
        <property type="entry name" value="HIG_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51503">
        <property type="entry name" value="HIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="HIG1" evidence="6">
        <location>
            <begin position="89"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="199"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR21283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="221" mass="25063" checksum="607DD937657B7891" modified="2009-07-28" version="1">MKILTEEEIEAHHYHTLSGGVKGTVAGLVISGLLFRYAPVKFPKFQPKRMPWSLKTAMFITPPTLLASIGAEEASTSFDNMMYGSGSASRDAIEEHRRWKSLPLSEKLTEGASNHKYKIIVAAWAASMWGSWKFVDRDPIMTKTQKAVQARMYAQGLTVMLLLGSIGLSMYEEKLHPDAKKLDRSKRWERLLERAEEEEARAQKEAGFRSNEDRVKAKIFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DC05</accession>
    <name>C5DC05_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ubiquitin-like modifier HUB1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0A06820g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551754.1">
        <property type="nucleotide sequence ID" value="XM_002551708.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DC05"/>
    <dbReference type="FunCoup" id="C5DC05">
        <property type="interactions" value="370"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DC05"/>
    <dbReference type="GeneID" id="8290561"/>
    <dbReference type="KEGG" id="lth:KLTH0A06820g"/>
    <dbReference type="eggNOG" id="KOG3493">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_156193_2_0_1"/>
    <dbReference type="InParanoid" id="C5DC05"/>
    <dbReference type="OMA" id="GMSLEMQ"/>
    <dbReference type="OrthoDB" id="3881at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000052">
        <property type="entry name" value="Ubiquitin-like protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039732">
        <property type="entry name" value="Hub1/Ubl5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13042">
        <property type="entry name" value="UBIQUITIN-LIKE PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="3">
        <location>
            <begin position="2"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00014108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR21312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="73" mass="8396" checksum="FAD8C85ED73C5A71" modified="2009-07-28" version="1">MIEVEVNDRLGKKIRVKCEGEDTVGDFKKVLALQLGISWTKIVLQKGGAVLKDHITLDDYEVHDQTYLELYYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DC59</accession>
    <name>C5DC59_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Ornithine aminotransferase</fullName>
            <ecNumber evidence="4 9">2.6.1.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0B00220g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 2-oxocarboxylate + L-ornithine = L-glutamate 5-semialdehyde + an L-alpha-amino acid</text>
            <dbReference type="Rhea" id="RHEA:13877"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:58066"/>
            <dbReference type="ChEBI" id="CHEBI:59869"/>
            <dbReference type="EC" id="2.6.1.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-ornithine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.13" evidence="4 9"/>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551808.1">
        <property type="nucleotide sequence ID" value="XM_002551762.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DC59"/>
    <dbReference type="FunCoup" id="C5DC59">
        <property type="interactions" value="898"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DC59"/>
    <dbReference type="GeneID" id="8290626"/>
    <dbReference type="KEGG" id="lth:KLTH0B00220g"/>
    <dbReference type="eggNOG" id="KOG1402">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016922_10_3_1"/>
    <dbReference type="InParanoid" id="C5DC59"/>
    <dbReference type="OMA" id="VCEGNFH"/>
    <dbReference type="OrthoDB" id="10261433at2759"/>
    <dbReference type="UniPathway" id="UPA00098">
        <property type="reaction ID" value="UER00358"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004587">
        <property type="term" value="F:ornithine aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019544">
        <property type="term" value="P:L-arginine catabolic process to L-glutamate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010121">
        <property type="term" value="P:L-arginine catabolic process to proline via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000011">
        <property type="entry name" value="Ornithine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000152">
        <property type="entry name" value="Ornithine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049704">
        <property type="entry name" value="Aminotrans_3_PPA_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050103">
        <property type="entry name" value="Class-III_PLP-dep_AT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010164">
        <property type="entry name" value="Orn_aminotrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01885">
        <property type="entry name" value="Orn_aminotrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986">
        <property type="entry name" value="AMINOTRANSFERASE CLASS III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986:SF18">
        <property type="entry name" value="ORNITHINE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="7 8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR21369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="463" mass="50500" checksum="4FF0C239BCA10370" modified="2009-07-28" version="1">MILARGKVAGLRRVFHHGGAFLASSQAFKMSTLNTDLSSETTVQYEKAYSAHNYHPLPVVFQKAKGAKVWDPEGKQYLDFLSAYSAVNQGHCHPDIIAALVDQASKLTLSSRAFSNNVFSAFAKFITEYFRYESVLPMNTGAEAVETGLKLARRWGYMKKGIPENEAIILGAKDNFHGRTLGALSLSTDEEARKFFGPFLESITAKIPGTDGQYLRYGNIEDVELAFKNAGDRIAAILLEPIQGEAGIVVPPVEYLQKVQELCRKYNVLLICDEIQTGIARTGKMLCYEHSPNVKPDVILLGKAISGGILPVSCVLSSKEIMGCFEPGSHGSTYGGNPLSSRVAIAALEVVKKENLVERAKTLGEFFQSELKKLQQEAGGIISDVRGRGLLTAIVIDPSKSNGRTAWDLCLLMKDQGVLAKPTHDHIIRLAPPLVISKEDLMKGVEAIRASLKLLPNAAKSNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DC64</accession>
    <name>C5DC64_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0B00352p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0B00352g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551813.1">
        <property type="nucleotide sequence ID" value="XM_002551767.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DC64"/>
    <dbReference type="FunCoup" id="C5DC64">
        <property type="interactions" value="258"/>
    </dbReference>
    <dbReference type="GeneID" id="8290632"/>
    <dbReference type="KEGG" id="lth:KLTH0B00352g"/>
    <dbReference type="eggNOG" id="ENOG502RJ72">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015392_0_0_1"/>
    <dbReference type="InParanoid" id="C5DC64"/>
    <dbReference type="OMA" id="LAMMQRD"/>
    <dbReference type="OrthoDB" id="2943660at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050675">
        <property type="entry name" value="OAF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069">
        <property type="entry name" value="OLEATE-ACTIVATED TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069:SF29">
        <property type="entry name" value="OLEATE-ACTIVATED TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00755">
        <property type="entry name" value="AFLATOXINBRP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="8">
        <location>
            <begin position="18"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="72"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="202"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="72"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR21374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="934" mass="106148" checksum="117266018C8E8198" modified="2009-07-28" version="1">MEHQHPIKIQKRNRPSFVCQECRRRKIKCDKKRPRCSRCVDTGLPCTYLASGSRSAADWHISGDETSIWTGESVTTTESRTPGESGAATSEAHSEALGDEQLALPQASTRCRCSCGSCCGYGTTFAYADGSGRRAPTSASAHEIYVNFGNPEDTMVAHEQMRYLHKPFSALAMMQRDHFLRAFAGSVTGVILTGMHSAPANGNPESFTYDGKLNSRQDSNNEGRGTQEIPVNVFMEEIKKTNMLMNRRRAGNDLIKLVFFQNPWSFDDNVGSTSAYPAELVSLVTNIQDILPDVAALRFLLDCFYEAVYPLHPFLDVPSFEKVLSEVLKARSDGSQRYNIMLGTSALHNKLENLAILLVVLKISQAALNMQQDPLKSATAKKLDIFGHNNIKDDVIILAQKCLNALNFLRLPNENTFCCLLFLWIYQSYDLDDVNFSLPPHVALVLGTLSHLSVILGLHNDPSMYPRLVESSQFHSSFLNYRRKLWMGVVSVRTNELLLNGNSLESTHALMSSFLKRGREDKESYMSSVIQDEGQENSFDTRLHEFAHKEYELRRIWTKLNSACNSLDKPILLSEIEIIISRAEITLSQLFPLTDITEISSFSCPEKGTVKCAESKAEIDYNAIRACKTYANNLRGRIIILNISMALTVHFENLCRNEPDRYLEYYRKYLKLTFRRSLEIVKLLKKLPDNMEKNSLFSRYKYALTRITLDSVLRTLIPMCSILFKLSFAERQLKMSVKSERVLYEKDDHSDSSHSLQMIVNVKESLAQALRSLISLQSTNWGLGFLSCYRLVCMAKYVLHLVDIDMLPEVTNRFWEYEKNYKQVPESIANRIFLKWGLKFKESKAIKDDLFNPNVLGAMDSDLLESFKTILESLELWKLTENTPEVNDVSSLSDRNEQLSEEVAEDAQGFSLDILDYFKVFEDEYSGESFPSIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCE6</accession>
    <name>C5DCE6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0B02420p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0B02420g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551895.1">
        <property type="nucleotide sequence ID" value="XM_002551849.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCE6"/>
    <dbReference type="FunCoup" id="C5DCE6">
        <property type="interactions" value="15"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCE6"/>
    <dbReference type="GeneID" id="8290725"/>
    <dbReference type="KEGG" id="lth:KLTH0B02420g"/>
    <dbReference type="eggNOG" id="KOG3223">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_186256_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCE6"/>
    <dbReference type="OMA" id="KWDQGSR"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054413">
        <property type="entry name" value="LSO1/2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22048">
        <property type="entry name" value="LSO1_2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LSO1/LSO2" evidence="2">
        <location>
            <begin position="10"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR21457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="88" mass="9782" checksum="356F791F393B5068" modified="2009-07-28" version="1">MGKRFSESAAKKAAGNQRKREQAAAKGRAEREKAESEEAAKWEEGSKKPSTKKQLEEQKKQEKLRAKQEREELLAAEEAALGRGGKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCK4</accession>
    <name>C5DCK4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">Squalene monooxygenase</fullName>
            <ecNumber evidence="4 10">1.14.14.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0B03784g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the stereospecific oxidation of squalene to (S)-2,3-epoxysqualene, and is considered to be a rate-limiting enzyme in steroid biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>squalene + reduced [NADPH--hemoprotein reductase] + O2 = (S)-2,3-epoxysqualene + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25282"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15440"/>
            <dbReference type="ChEBI" id="CHEBI:15441"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="1.14.14.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Endoplasmic reticulum membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the squalene monooxygenase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.17" evidence="4 10"/>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551953.1">
        <property type="nucleotide sequence ID" value="XM_002551907.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCK4"/>
    <dbReference type="FunCoup" id="C5DCK4">
        <property type="interactions" value="205"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCK4"/>
    <dbReference type="GeneID" id="8290787"/>
    <dbReference type="KEGG" id="lth:KLTH0B03784g"/>
    <dbReference type="eggNOG" id="KOG1298">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026390_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCK4"/>
    <dbReference type="OMA" id="AKRTFYW"/>
    <dbReference type="OrthoDB" id="1678617at2759"/>
    <dbReference type="UniPathway" id="UPA00767">
        <property type="reaction ID" value="UER00752"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004506">
        <property type="term" value="F:squalene monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013698">
        <property type="entry name" value="Squalene_epoxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040125">
        <property type="entry name" value="Squalene_monox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10835">
        <property type="entry name" value="SQUALENE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10835:SF0">
        <property type="entry name" value="SQUALENE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08491">
        <property type="entry name" value="SE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 10" id="KW-0274">FAD</keyword>
    <keyword evidence="5 10" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0492">Microsome</keyword>
    <keyword evidence="8 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="443"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="473"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="Squalene epoxidase" evidence="11">
        <location>
            <begin position="202"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU367121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR21515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="497" mass="54990" checksum="61A13A5AFE2BCFBC" modified="2009-07-28" version="1">MSGGKATNTDLKNADASITYDAIIVGCGVIGPCVATGLARKGKKVLIVEREWSMPDRIVGELMQPGGVRALRSLGMVQSINDIEAIPVTGYTVFYNGEKVAIPYPYKADIAPVEKVPKLVRDGNDKVTEDKTIHVKDFEEDERERGVGFVHGKFLQNLRAIVAEEPNVTRLQGNVVEILKNGHNEVVGAKVDIDGRGKVEFKAHITFVCDGIFSKFRKELSTDHVPTVGSSFVGMSLFHAKMPSPNHGHVILGTEHMPILVYQISPEETRILCAYNSPKLPSDVKAWMTKKVQPYIPESLRPSFDEALAQGKFRSMPNSYLPGRQNNVIGMCVIGDALNMRHPLTGGGMTVGLNDVVLLMKKIGDLDFSDREVVLDELLDYHYERKSYDSVINVLSIALYSLFAADSKDLKVLQKGCFKYFKRGGDCVKLPVSFLSGVMPRPFLLTRVFFAVAFYAVYVNFQERGMAGFPVALIEAFSILFTAARVFTPYLYTEMIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCQ1</accession>
    <name>C5DCQ1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">proline--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Prolyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0B04906g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>tRNA(Pro) + L-proline + ATP = L-prolyl-tRNA(Pro) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:14305"/>
            <dbReference type="Rhea" id="RHEA-COMP:9700"/>
            <dbReference type="Rhea" id="RHEA-COMP:9702"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78532"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.15"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.15" evidence="2"/>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552000.1">
        <property type="nucleotide sequence ID" value="XM_002551954.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCQ1"/>
    <dbReference type="FunCoup" id="C5DCQ1">
        <property type="interactions" value="459"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCQ1"/>
    <dbReference type="GeneID" id="8290838"/>
    <dbReference type="KEGG" id="lth:KLTH0B04906g"/>
    <dbReference type="eggNOG" id="KOG2324">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016739_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCQ1"/>
    <dbReference type="OMA" id="NCDYAAN"/>
    <dbReference type="OrthoDB" id="10267474at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004827">
        <property type="term" value="F:proline-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006433">
        <property type="term" value="P:prolyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000156">
        <property type="entry name" value="YER087W-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002316">
        <property type="entry name" value="Pro-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004500">
        <property type="entry name" value="Pro-tRNA-synth_IIa_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050062">
        <property type="entry name" value="Pro-tRNA_synthetase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00409">
        <property type="entry name" value="proS_fam_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42753">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOME PROTEIN L39/PROLYL-TRNA LIGASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42753:SF2">
        <property type="entry name" value="PROLINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01046">
        <property type="entry name" value="TRNASYNTHPRO"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="10">
        <location>
            <begin position="54"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR21562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="568" mass="63549" checksum="9D9B3F4298E05445" modified="2009-07-28" version="1">MRPCLKTIVRNVHVYQTKSLTGDAVKASSTHNLLQSLGYIKQTQSGLVHWLPLGLRTLNKITNIIKHRMETDGKAEEVSLSLLSPKSLWVHTNRWDNGELFKLKDSKGSDYCLAPTCEEEITSLIGSYVTSYKDLPMTVYQVGRKYRDEKRPRGGLLRGREFLMKDAYSFDASKEEAVRTFESMNHTYDLIFKDLGVPFVSAWADSGDIGGDMSKEYHYTHESGEDTVMACDSCGHISNVEKCSSFPVEDNQYSGDVSVRYALSEDRDTLVCLYYPQNRVLNWNLVKAALDGDVDSSLRDTSNEKVLELFRANKSEDEMMLASVLRVMDCRLNSRSNYPDFPLNQYLKSNFSQLSDASIVDAEAGELCGSCEEGHLSSKRSIEVGHTFFLGQKYSEPLNAKFSTRENTEEYCEMGCYGIGVSRLVGAIAHVTRDDAGLRWPRAVAPYEISICAAKQDELVAEVEGLLSSWGREVWVNRDEKAGLGRKIAASHSLGIPVCVIVGKKSWPCVEIEVRAPLVGERWRAELAQHGEAWGWTVQESAPVGGAAHHRVRKEHLKDVVGVILTDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCS8</accession>
    <name>C5DCS8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">KLTH0B05500p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0B05500g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Tom22 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552027.1">
        <property type="nucleotide sequence ID" value="XM_002551981.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCS8"/>
    <dbReference type="FunCoup" id="C5DCS8">
        <property type="interactions" value="310"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCS8"/>
    <dbReference type="GeneID" id="8290865"/>
    <dbReference type="KEGG" id="lth:KLTH0B05500g"/>
    <dbReference type="eggNOG" id="KOG4111">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094333_1_0_1"/>
    <dbReference type="InParanoid" id="C5DCS8"/>
    <dbReference type="OMA" id="LVWIVTT"/>
    <dbReference type="OrthoDB" id="10016939at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22884">
        <property type="entry name" value="TOM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005683">
        <property type="entry name" value="Tom22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12504">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12504:SF0">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM22 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04281">
        <property type="entry name" value="Tom22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="37"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR21589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="145" mass="16110" checksum="559455522A26A731" modified="2009-07-28" version="1">MVELTEVREENISFEEEKVPSSSFSRQDTTAEHESSDEFSSDDEYDEEETFLERLVALKDIIPPRQRQTISNVFNVTVSAVRNTFSKSGNVAWAVTTSALLLGVPLSLSILAEQQLIEMEKSFDLQKDANEILVQGDATPAPAIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCU9</accession>
    <name>C5DCU9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0B05984p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0B05984g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552048.1">
        <property type="nucleotide sequence ID" value="XM_002552002.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCU9"/>
    <dbReference type="FunCoup" id="C5DCU9">
        <property type="interactions" value="39"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCU9"/>
    <dbReference type="GeneID" id="8290887"/>
    <dbReference type="KEGG" id="lth:KLTH0B05984g"/>
    <dbReference type="eggNOG" id="ENOG502QUAB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012105_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCU9"/>
    <dbReference type="OMA" id="FYHIDES"/>
    <dbReference type="OrthoDB" id="5865767at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37283">
        <property type="entry name" value="PH DOMAIN-CONTAINING PROTEIN YHR131C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37283:SF1">
        <property type="entry name" value="PH DOMAIN-CONTAINING PROTEIN YHR131C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="304"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="410"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="472"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="528"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="304"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="354"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="419"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="446"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="544"/>
            <end position="557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="722" mass="80774" checksum="CD18BE886DF2F5A1" modified="2009-07-28" version="1">MSEVANARKSKYIKHTSIGKTKSACSSTSISSTEGTGKIFEAIDKPLKDPMVKVKKSKSDPGRGAEKAGASEEDIFAWIDPQPAKPPSYKSANPNRRITYPVYETQDSGVLPAYSPSVEEVTVVSMKMEWLDPYNCSPSRSWKNFIMEINSTQLNFYLIDPSLTRNIKNYCNGKSHFGGESVDPDIEYEGHHSIFNSLASKGTYQFNKADQESISQRITKDKAKYLTNNRLFRTYSLQFAKFGIPTDYSRKTFVLRLRCETEQFMLSFSHVDDMIMWSMYLSIGIGVALDLDFRELPTYRTVPRRRRRRRRKRTNALTSSGGNDRSGSDRRSFSNSSTRNGVLELHYPYKKRPNSMSNPDSHSNSSSRRGSNESIKSRLKSFFGSDKKAPCPSFRGPYPRALSTVGLNAVTEDEEEEQVPSTSASASNSPQKTEIPVRPSLEQRSKSLGTLQSSSNADDYFGFNHRGEDARDIAVTTPGSPGSPGSEVSIGGATIASGELQLQSGYHNNVGLQNDLDELQQVIYEHNGEEGEETEDSPTFGNTDNDDEGEEEDDDDIDSLHPHTATAATSIYQEEGIFHDSEDDYYYVVDRGDAFRRRASSVTSNLSSIPYGSCEVKWHPPRKEMSRRRYIRDSLRCIRPLPEDEEWLGKVLICPTQAPAYETNNPPISGYILGGGKPGKSKAPKFRNFKTDKGVILNKCKNHFVKPYIVGPVGFLKTNARC</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCY2</accession>
    <name>C5DCY2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0B06754p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0B06754g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAH family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552081.1">
        <property type="nucleotide sequence ID" value="XM_002552035.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCY2"/>
    <dbReference type="FunCoup" id="C5DCY2">
        <property type="interactions" value="493"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCY2"/>
    <dbReference type="GeneID" id="8290920"/>
    <dbReference type="KEGG" id="lth:KLTH0B06754g"/>
    <dbReference type="eggNOG" id="KOG1535">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028458_5_0_1"/>
    <dbReference type="InParanoid" id="C5DCY2"/>
    <dbReference type="OMA" id="QSERICV"/>
    <dbReference type="OrthoDB" id="74910at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018773">
        <property type="term" value="F:acetylpyruvate hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.850.10">
        <property type="entry name" value="Fumarylacetoacetase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011234">
        <property type="entry name" value="Fumarylacetoacetase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036663">
        <property type="entry name" value="Fumarylacetoacetase_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11820">
        <property type="entry name" value="ACYLPYRUVASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11820:SF7">
        <property type="entry name" value="ACYLPYRUVASE FAHD1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01557">
        <property type="entry name" value="FAA_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56529">
        <property type="entry name" value="FAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950418" evidence="3">
        <location>
            <begin position="21"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="Fumarylacetoacetase-like C-terminal" evidence="4">
        <location>
            <begin position="84"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR21643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="334" mass="37207" checksum="FD57E541822A8203" modified="2009-07-28" version="1">MFKVFYILILWFYCFTCFRCAPIRPVGTGRWRLGILRGCTYLGIFSRLFGSSHKGSLTTTHQQKLGKGCHQSKMDYSYLKAARKIVCIGRNYAAHVKELNNPFPKQPFFFLKPSSSIVTPHDKQAPQETRQHAECSYRGLNADGTNPGAIMIPEGVKVHHEVELALIIGDYISNASPSAFGPKELRRAISGIALSLDLTARNVQDEAKRKGLPWSIGKGFDTFLPMSKFIPEARLGKNLDLQDAFRLKCTVNSITRQDGTTGLMLTPLHTIVQHISTMMSLEPGDIILTGTPAGVGELKPGDSVYSELFFNGEPLVNMQFDCVKRPGPYVYRET</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>C5DD29</accession>
    <name>C5DD29_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0B07832p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0B07832g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552128.1">
        <property type="nucleotide sequence ID" value="XM_002552082.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DD29"/>
    <dbReference type="FunCoup" id="C5DD29">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="GeneID" id="8290968"/>
    <dbReference type="KEGG" id="lth:KLTH0B07832g"/>
    <dbReference type="eggNOG" id="ENOG502S73A">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_177980_0_0_1"/>
    <dbReference type="InParanoid" id="C5DD29"/>
    <dbReference type="OMA" id="RILFWDE"/>
    <dbReference type="OrthoDB" id="4138121at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020301">
        <property type="entry name" value="Mrx7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10906">
        <property type="entry name" value="Mrx7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR21690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="86" mass="10492" checksum="8A7077FADBB5B628" modified="2009-07-28" version="1">MMRGSPRSIEEFLYRTLMNSEGFHRFVRRIYYKVNGLEEPMSGRPAAHVEQVFRPTRLQKFKAYRILFWDEMRAVVGLNRRFNRHL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-05-03" modified="2025-06-18" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>C5DD31</accession>
    <name>RRG9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName>Required for respiratory growth protein 9, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">RRG9</name>
        <name type="ordered locus">KLTH0B07876g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for respiratory activity and maintenance and expression of the mitochondrial genome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RRG9 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552130.1">
        <property type="nucleotide sequence ID" value="XM_002552084.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DD31"/>
    <dbReference type="SMR" id="C5DD31"/>
    <dbReference type="FunCoup" id="C5DD31">
        <property type="interactions" value="43"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DD31"/>
    <dbReference type="GeneID" id="8290970"/>
    <dbReference type="KEGG" id="lth:KLTH0B07876g"/>
    <dbReference type="eggNOG" id="ENOG502S7IA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100293_0_0_1"/>
    <dbReference type="InParanoid" id="C5DD31"/>
    <dbReference type="OMA" id="WREDTKI"/>
    <dbReference type="OrthoDB" id="5578174at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010487">
        <property type="entry name" value="NGRN/Rrg9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13475">
        <property type="entry name" value="NEUGRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13475:SF3">
        <property type="entry name" value="NEUGRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06413">
        <property type="entry name" value="Neugrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0496">Mitochondrion</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0809">Transit peptide</keyword>
    <feature type="transit peptide" description="Mitochondrion" evidence="2">
        <location>
            <begin position="1"/>
            <end status="unknown"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000407949" description="Required for respiratory growth protein 9, mitochondrial">
        <location>
            <begin status="unknown"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="166"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="223" mass="25812" checksum="795CEF2D53F11832" modified="2009-07-28" version="1" precursor="true">MKPVLQGALAWIYAGYKTASRGHLLPYTRHQKFHSSSLISEQAKRAKDLIKLVNSSSLSNSESQSLDWRSDLKVPEWKRQKLALKDKFKGQQWNPKKKLSREQMENVRLLKRHFPETSATELSERFQVSPEVIRRILKSKWQPNEEEQLQLQTRWKRRSERVNEILGSPEHQKLPPKKLVLGSGRTDTDLQVKSVRRTAVKAGRNSSSPKGKQKLNLLSKLIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEA0</accession>
    <name>C5DEA0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0C07480p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0C07480g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552549.1">
        <property type="nucleotide sequence ID" value="XM_002552503.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEA0"/>
    <dbReference type="FunCoup" id="C5DEA0">
        <property type="interactions" value="85"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEA0"/>
    <dbReference type="GeneID" id="8291420"/>
    <dbReference type="KEGG" id="lth:KLTH0C07480g"/>
    <dbReference type="eggNOG" id="ENOG502QW5G">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014489_1_0_1"/>
    <dbReference type="InParanoid" id="C5DEA0"/>
    <dbReference type="OMA" id="WYDISHQ"/>
    <dbReference type="OrthoDB" id="424974at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF49">
        <property type="entry name" value="LYSINE BIOSYNTHESIS REGULATORY PROTEIN LYS14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="4">
        <location>
            <begin position="148"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="21"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="184"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="23"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="61"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="87"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="117"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="211"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR22111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="775" mass="86532" checksum="8255DA06BA04CDE8" modified="2009-07-28" version="1">MQSFAKSMVLFNDADGAKEALVSGGSPTSYLSPSSISNESNQGQSVRDSIFTQQLSSERFSGNSYSSGPSSICAERSPAVDGIAGTGSVTPLSSTKNDSPNNLGMGIGTGTPDAESHQGSSATSTLASQPASSNSNGKTVKRKYSRNGCTECKRRRMKCDEGKPTCWQCARLNRECVYIIRTKNRKRRPKSTEASKESAQADALVRESNAPGLTTNPSGGSPFSNTSTDLHSTLHNPLNTSLPVENLSSSMPELPTVIPTNNVNAYDANLLIRNLNDIVNMKLNDSMIHDDLKSMDFSDLDFPDLTAIPQQSTYAAVPISFLVDSVMTFNMSLDSFKLGATHDKFLEVFYYDCLDSVAPFFQNQGNPLRDILLSFARGESYLLSAILAVGASISHRKSNNVEDEKSYCAYLSHCLGLLTEQFKEESNVFNKIEPIILTVLMLTWDCIYTMNSQWRSHLKGVTELFKKINSGSSSKVLNVAKCWFKVIETFASISTVLGGALVDETDLDVIFDPYDYQYIDSLKFLNIMTPLNEFNLLRGHKEDFDLVIKEVFKALNVIRHSEKKYFSEQEGIFDKNLDYLLWSNPNTDTFKQQLSYFKIQKILVEIDRQSEYVFIDKSGVIPTTSPSHPKNSKIMDNAIDLVKLHSGEEIAISWYDISHQTQVLSFLLIVLLKLLGIPKESIAIQQVVQKITSFFGFLDSENPPQNLRTCYCNFAILIAGLNAIDENTRKVIKSYYKLNGGRFQRLTEHNLNRLEKVWYGNEKTYKLEDQDVLTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEB7</accession>
    <name>C5DEB7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0C07876p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0C07876g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552566.1">
        <property type="nucleotide sequence ID" value="XM_002552520.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEB7"/>
    <dbReference type="FunCoup" id="C5DEB7">
        <property type="interactions" value="136"/>
    </dbReference>
    <dbReference type="GeneID" id="8291438"/>
    <dbReference type="KEGG" id="lth:KLTH0C07876g"/>
    <dbReference type="eggNOG" id="ENOG502S6BG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_460090_0_0_1"/>
    <dbReference type="InParanoid" id="C5DEB7"/>
    <dbReference type="OMA" id="ANKRICA"/>
    <dbReference type="OrthoDB" id="4035955at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="200"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="204"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="221"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="593" mass="66716" checksum="0DE46FD8279C532A" modified="2009-07-28" version="1">MAVRTIGPHQPLSTNCSSDVKRQEEKQAGSKRKQFRKWLHSLKEHAPSPDYRDEETVLIDIADSYLGFLDPLSYVPVLTTGEAAMLDCEKQSPSRTHSKRLREFLREHLKRRPSTHKDGRALQSRDQGPESSLNMSCSEANEAVVGDSPSQLDRPVCENEFTILKSEAVEGLAPIKQEDDSQGSRSADGLKEPCFILQQDEDAGQNSLKRTSDSPLADNSTESKRTCFEREPERNDDGFIDESSSVYSDVPSPEASICKTPPAVLTKPCEGNVTPSRGAGDDSNSAPLEDKLYVVIEKLKAAVHPRSSGSAKFEPADCSASSDKIASPVYEPTFIRVPTFRQSLRRMHARGLLEDVRNGTIKEEDLLKLAKREPESFNFRDAKFFEKDSEASGSSESSKSLESSRFYSVKFDKFSHLLMYDATKRFHSDRWGTLRSANLSTGSKGKAKPCPLLENAVYSKESIRSRKPILKCKSNTRAEVESLRAEDCDKVDVFAFLKFFEHHEAQRRSEELSLSRIREQQLTNYYANDHVYNSARSGSLKFAAVDFNRSKKATEINIGRELRGPDRRISSCPSMRDWDLGRKRRGDVSQHCY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEL0</accession>
    <name>C5DEL0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0C10120p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0C10120g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552659.1">
        <property type="nucleotide sequence ID" value="XM_002552613.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEL0"/>
    <dbReference type="GeneID" id="8291539"/>
    <dbReference type="KEGG" id="lth:KLTH0C10120g"/>
    <dbReference type="eggNOG" id="KOG0254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_22_1_1"/>
    <dbReference type="InParanoid" id="C5DEL0"/>
    <dbReference type="OMA" id="IVMARIN"/>
    <dbReference type="OrthoDB" id="10021397at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17502">
        <property type="entry name" value="MFS_Azr1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000373">
        <property type="entry name" value="Vacuolar basic amino acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1720.10">
        <property type="entry name" value="Multidrug resistance protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF198">
        <property type="entry name" value="AZOLE RESISTANCE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="41"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="79"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="110"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="134"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="167"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="195"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="263"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="295"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="341"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="383"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="407"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="437"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="482"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="45"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="603"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="604"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="639"/>
            <end position="651"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR22221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="651" mass="70105" checksum="6140729609AB4440" modified="2009-07-28" version="1">MLIYLFRRLRSAAAKTRTDTQAQTAPSHSFSLQDFSAKHALLALCLLSLVLTLFLAALDIVIVVTLYETIGEKFNDYNDVGWLVTGYSLSSALFTLLWGRMASLLGLKTCLMLSVLIFEIGSLIAAVSNSMGMLIAGRVVAGAGGSGIQSLVYIVGTSLVSERSRGMVIMVLTFAFVGSNAGGPILGGALNEHASWRWCFFINLPIGGAAAFMLFLCYNPSGKPLLSTLTSKLEAARDYRYSNVCTRKFWAHALDLGVFRFDIIGFALSSAGFVLLLLGLTFGGRKYSWSSGTTIAYLTVGPVLIVVFCLYDFLVLPVLARSLAYSSEVTPLVPWPVISKPGVFATSFASFFNFFAYNMQIIYLVQFYQVVHNESPTNASTHMWALSIPALVVIIVLGRINKKFGILKPVAVIGAACGTIGAGLLTLLKGSTTSGQSIGYCILAGAGFSAVMQSTLLSAQIQVDKTDPKFNQKFIEITTLNNFFRVLGISFGGIMGTLIYSTSLKNELATAGPNIPSFASTDELIAYRSKNFDGASSPLENIMSKSIQRVFYGSLGCQALAFLFTICMSNKRLDLEPNPPAVATFEPALNAAVSDQEKGFGITQGPTSSETQNLRVSDSSLAYNITGEKADENEEHYDSMTSRDSPVSAKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DER7</accession>
    <name>C5DER7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0C11528p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0C11528g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552716.1">
        <property type="nucleotide sequence ID" value="XM_002552670.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DER7"/>
    <dbReference type="FunCoup" id="C5DER7">
        <property type="interactions" value="674"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DER7"/>
    <dbReference type="GeneID" id="8291603"/>
    <dbReference type="KEGG" id="lth:KLTH0C11528g"/>
    <dbReference type="eggNOG" id="KOG1230">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008722_0_0_1"/>
    <dbReference type="InParanoid" id="C5DER7"/>
    <dbReference type="OMA" id="PSPRVGC"/>
    <dbReference type="OrthoDB" id="4447at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025183">
        <property type="entry name" value="DUF4110"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052588">
        <property type="entry name" value="Kelch_domain_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46063">
        <property type="entry name" value="KELCH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46063:SF1">
        <property type="entry name" value="KELCH DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13422">
        <property type="entry name" value="DUF4110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4110" evidence="2">
        <location>
            <begin position="554"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="494"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="499"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR22278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="642" mass="74448" checksum="FD1F6A5F9E81B785" modified="2009-07-28" version="1">MAKKNKKDKEAKKARAELKNKKNLEKAEVKDKKRSKKLNSEDDDDLDIDEILENFRKEQELFQEVVVTPVKRPSPRSNVCMVPNPSHGKRELLIFGGEYTNQTSSTTTFYNELFTYSLDTDQWKKITSRNAPMPRSSAAAAGHPSGVVLIHGGEFSSPKQNTFYHYSDSWLLDCSSKEWTKIDQKNGPSARSGHRMTVWKNFFVMHGGFRDLGTSTTYLNDCWLFDITTYKWKQVEFPPNHPVPDARSGHSLIPTQEGAIVWGGYCKIKAKKGLQKGKILTDCWYLRMKSDITGIRWERRKKQGFQPSPRVGCSMAHHKGRGILFGGVYDYEETEEGLSSEFFNDLLSYQVEANRWYSIKMRPQKKKIVAASKSAKSKDDELVGILNDILQKANLADEENEENESDSEIEKELHRLDEEELLGHQQKQYPVVAQLPHSRFNAATTVVDDTLFIFGGSWEYGERDFSIDSFYSIDLNRTDGVRVFWEDLEEIEKAKDQGLEEDEEEEEEEDDDDEDDEEETLDEELIAEMEDDKQEEEEQEEEEEEEEAGPEIPDPRPWLPHPKAFETLRAFYIRTGAEFLQWAISNNRSAKGKHLKKQSFDLSQDRWWERREQVRIEEDKLEEMGGVENIIERDSSHSTKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DF09</accession>
    <name>C5DF09_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Trimethylguanosine synthase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Cap-specific guanine-N(2) methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0D11330g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)-ribonucleoside in snRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(2),N(7)-trimethyl 5'-triphosphoguanosine)-ribonucleoside in snRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:78479"/>
            <dbReference type="Rhea" id="RHEA-COMP:19087"/>
            <dbReference type="Rhea" id="RHEA-COMP:19089"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:167623"/>
            <dbReference type="ChEBI" id="CHEBI:172880"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:78480"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(2),N(7)-trimethyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:78507"/>
            <dbReference type="Rhea" id="RHEA-COMP:19088"/>
            <dbReference type="Rhea" id="RHEA-COMP:19090"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:167623"/>
            <dbReference type="ChEBI" id="CHEBI:172880"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="3">
            <dbReference type="Rhea" id="RHEA:78508"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in snRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)-ribonucleoside in snRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:78471"/>
            <dbReference type="Rhea" id="RHEA-COMP:19085"/>
            <dbReference type="Rhea" id="RHEA-COMP:19087"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:156461"/>
            <dbReference type="ChEBI" id="CHEBI:172880"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:78472"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:78475"/>
            <dbReference type="Rhea" id="RHEA-COMP:19086"/>
            <dbReference type="Rhea" id="RHEA-COMP:19088"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:156461"/>
            <dbReference type="ChEBI" id="CHEBI:172880"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:78476"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyltransferase superfamily. Trimethylguanosine synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553202.1">
        <property type="nucleotide sequence ID" value="XM_002553156.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DF09"/>
    <dbReference type="FunCoup" id="C5DF09">
        <property type="interactions" value="137"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DF09"/>
    <dbReference type="GeneID" id="8295442"/>
    <dbReference type="KEGG" id="lth:KLTH0D11330g"/>
    <dbReference type="eggNOG" id="KOG2730">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029658_2_0_1"/>
    <dbReference type="InParanoid" id="C5DF09"/>
    <dbReference type="OMA" id="KALCIYY"/>
    <dbReference type="OrthoDB" id="194443at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071164">
        <property type="term" value="F:RNA cap trimethylguanosine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019012">
        <property type="entry name" value="RNA_cap_Gua-N2-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14741">
        <property type="entry name" value="S-ADENOSYLMETHIONINE-DEPENDENT METHYLTRANSFERASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14741:SF32">
        <property type="entry name" value="TRIMETHYLGUANOSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09445">
        <property type="entry name" value="Methyltransf_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="275"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00025783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00047418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR22764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="302" mass="34611" checksum="CCA424556F1727CE" modified="2009-07-28" version="1">MGRLNSSELEHFVHLGKRKYRRERRALKKLFRTNEHRVEPDVKVVDRTIFKYWKNRLTLFSRMRNRDIYMTRELWFSVTPESVAKFVARFIKSCLPEAKVVLDVYCGGGGNTIQLAQYFPKVYGVDNSLDHLYCAYRNAQAYGVEDRVWLKFGDWRVLSRKRRFEKVAVDCVFSSPPWGGPEYLRSEVYDLEAALQPQGLRETLAGLLRISENVVLFLPRNADLSQLSRVTRELLGEQALCKVLYVKQNGYMKGILCFWGARFYNYAGSTAEPSGAAQESSGVAEPCEVAPGADTLDYSVDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>C5DF56</accession>
    <name>C5DF56_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">tRNA dimethylallyltransferase</fullName>
            <ecNumber evidence="5 6">2.5.1.75</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0D12386g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>adenosine(37) in tRNA + dimethylallyl diphosphate = N(6)-dimethylallyladenosine(37) in tRNA + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:26482"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10375"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57623"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74415"/>
            <dbReference type="EC" id="2.5.1.75"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 7">Belongs to the IPP transferase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.75" evidence="5 6"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553249.1">
        <property type="nucleotide sequence ID" value="XM_002553203.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DF56"/>
    <dbReference type="FunCoup" id="C5DF56">
        <property type="interactions" value="1034"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DF56"/>
    <dbReference type="GeneID" id="8295489"/>
    <dbReference type="KEGG" id="lth:KLTH0D12386g"/>
    <dbReference type="eggNOG" id="KOG1384">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032616_2_3_1"/>
    <dbReference type="InParanoid" id="C5DF56"/>
    <dbReference type="OMA" id="VPHYLID"/>
    <dbReference type="OrthoDB" id="775260at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052381">
        <property type="term" value="F:tRNA dimethylallyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00185">
        <property type="entry name" value="IPP_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039657">
        <property type="entry name" value="Dimethylallyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030666">
        <property type="entry name" value="IPP_transferase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018022">
        <property type="entry name" value="IPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00174">
        <property type="entry name" value="miaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11088">
        <property type="entry name" value="TRNA DIMETHYLALLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11088:SF89">
        <property type="entry name" value="TRNA DIMETHYLALLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01715">
        <property type="entry name" value="IPPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039110">
        <property type="entry name" value="IPP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 6" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="353"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR039110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR22811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="436" mass="50597" checksum="8E2967DC5A5CD229" modified="2009-07-28" version="1">MLRWISQKLAMSAVPRPKVIVIAGTTGVGKSQLSIQLASRVSGEVINSDSMQVYKDIPIITNKHPIEERENVAHHVMNHVSWDEEYFLHRFEKECLNAIKDIHSRGKIPIIVGGTHYYLQILLEKRIEKKTRALLPEEQAILDSNEPHQLYSMLKEVDPDIASKYHPNDIRRVRRMLEIYFTTGQKPSQAFSEQKQSLVFDTLFLWVYSSPEVLEGRLDKRVDAMMESGALNEIRQLHEYYNSCNYSVDKCENGVWQVIGFKEFLPWLENADGSSFCDSVDRMKVRTRQYAKKQVKWIKKMLLPDVKSHLYALDATDLNHWDENVSQRAFSIADSFISNRIIEEPFVPPSLEDVVKNNDTESNSPKTAGDWSRQQCDICRDKDDNPLVAIGEKSWNIHLKSRRHRSNLSRIKKRENLELWKKKKAKEICADEKEAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFM4</accession>
    <name>C5DFM4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0D16302p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0D16302g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553417.1">
        <property type="nucleotide sequence ID" value="XM_002553371.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFM4"/>
    <dbReference type="FunCoup" id="C5DFM4">
        <property type="interactions" value="24"/>
    </dbReference>
    <dbReference type="GeneID" id="8295664"/>
    <dbReference type="KEGG" id="lth:KLTH0D16302g"/>
    <dbReference type="eggNOG" id="ENOG502QW7F">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_698432_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFM4"/>
    <dbReference type="OMA" id="MGESFVY"/>
    <dbReference type="OrthoDB" id="4073891at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="233"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="276"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="325"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="381" mass="42871" checksum="26222C72C6215808" modified="2009-07-28" version="1">MNLKTILVQKQTLICTWVILLTLVFVLLLLYLPSVSCTSVTGPRFCIPQYKVRFLDVLDEDRNLLQGLKDLLQLLSYVAVDLSSSSSMPATEDYDSINLVNTFDASNTFKINYLGFCKQQLTRSKIPAYCSNNRNGLEPVSALVRDVGIQFGIVASKNPHIMGESFVYVFKMGLSTLLASPEDFPGKESAISKYLSTRATNGGESLKSAGETFHRLEELAYWLSMMQRFCWTLGWAGLIEFFLCVIILISLLLAAPANLFGKWRKHMSWFEAMGTLLLKSLPILVLLVSFFDLLFSATWYISLKKASSHYGLTHKFYDLKLGEGFYINVCRLFFQCVILFQALRFTRNITLEVHSDEEHTEIGSPQKDLEAGSVLSSGTTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFN0</accession>
    <name>C5DFN0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0D16434p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0D16434g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553423.1">
        <property type="nucleotide sequence ID" value="XM_002553377.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFN0"/>
    <dbReference type="STRING" id="559295.C5DFN0"/>
    <dbReference type="GeneID" id="8295670"/>
    <dbReference type="KEGG" id="lth:KLTH0D16434g"/>
    <dbReference type="eggNOG" id="KOG3771">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_609830_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFN0"/>
    <dbReference type="OMA" id="FLGNCPE"/>
    <dbReference type="OrthoDB" id="10255128at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031097">
        <property type="term" value="C:medial cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990528">
        <property type="term" value="C:Rvs161p-Rvs167p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051666">
        <property type="term" value="P:actin cortical patch localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097320">
        <property type="term" value="P:plasma membrane tubulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00174">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046982">
        <property type="entry name" value="BIN3/RVS161-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47174">
        <property type="entry name" value="BRIDGING INTEGRATOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47174:SF1">
        <property type="entry name" value="REDUCED VIABILITY UPON STARVATION PROTEIN 167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="3">
        <location>
            <begin position="396"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR22985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="461" mass="52457" checksum="B8C7D094BDA2AF0A" modified="2009-07-28" version="1">MLYSKLASRGRVDQSARGPVEFRDFYQADEYVSRAPLLPAARESAEPHLRDRELERLTVKFREVQSHISTLSQCCVNYSKGVHAFVKHCVKINRQFEILLNSAQSWTTLCSTTSASKRGDYPTTSPLEPFLEKANDSDGLTFAFSEMNQSIRDPSRHQRRQEVALRLIEADLRIVKQCLVEPLLSIAEICKRVGRTLQRREVLAVAVKERMERLAWLENRTRGASPLDRRQQRKKARVARDLELEKLKLESLSTTIKVELKVLFKLFDAFVTTWAPNFFLTIFSIAYTMHRYLGRAGVDESITHQQALALLSPSEPGQFKQHSLKKRVAQTPSDISCGLPSIADIVASFHLDFDSVVSLLGNFRVTSFESLYQLTLSSATESAKSYFQDNAVRGTVPTLYATAIFGFSSTEPTHSDDLSFCRNDIIKVIKRNENGWWYGEVIRTKQRGYFPASCVQLEKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFN4</accession>
    <name>C5DFN4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">KLTH0D16544p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0D16544g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553427.1">
        <property type="nucleotide sequence ID" value="XM_002553381.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFN4"/>
    <dbReference type="FunCoup" id="C5DFN4">
        <property type="interactions" value="1172"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFN4"/>
    <dbReference type="GeneID" id="8295675"/>
    <dbReference type="KEGG" id="lth:KLTH0D16544g"/>
    <dbReference type="eggNOG" id="KOG1237">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004790_4_3_1"/>
    <dbReference type="InParanoid" id="C5DFN4"/>
    <dbReference type="OMA" id="LSECFCN"/>
    <dbReference type="OrthoDB" id="8904098at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071916">
        <property type="term" value="F:dipeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000085">
        <property type="entry name" value="MFS peptide transporter Ptr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000109">
        <property type="entry name" value="POT_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018456">
        <property type="entry name" value="PTR2_symporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00854">
        <property type="entry name" value="PTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01023">
        <property type="entry name" value="PTR2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0571">Peptide transport</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="118"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="150"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="177"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="237"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="262"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="432"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="476"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="509"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="542"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="18"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR22989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="626" mass="68851" checksum="6950CEC81C66393A" modified="2009-07-28" version="1">MSDHEKLSSVEKSQVAVLETDTYSEGGSGTPLSLPEPTEHERTTLRRVLEHPKWYVYFICLIEFSERASYYGTGDRLNNFIQQPLPAGGNGAGAPAHGAAGDSATAGALGMGLQTATALNLLLTFLAYCFPLVTGYIADRYWSRMRMLWIGVFVGIISHVIFIIGALPPVLGNGQAALAPCVIGIITLAIATSFVKPVLLPTLLSQYPHETDVVRTLASGERVLVDRDASLQRMTMTFYWSINIGSFLSVATSYAARDIGYWLAFLIPLILYLIMPAVLLVLGRYLRDEPPSGRSMLADCCKVLRVCFERGWWRRYRSGDFWNYARPSRLRQAGRAGWRRKGAGGFYAESLVQDTRVTLSASAIFLYYVIYNINDGGIGSIMNSQTASLTSNGVPNDLISNFNPLCIIVFMPVLDHVLYPVLRKMRWNFKPVYRIFCGFMIAALASAVGAIIQTVIYNTSPCGRYATECDKGVSPVSMWVVAVEYALTGLSECLAMTTGYEIAFERSPAHMKSFVMALFLFTSALSAALGEIVTPSLVDPHLVIPFGVLAGLGAFMAFVFLWRYWSLDKTMELERAERRALEAEEGAMPAITSVRSYVSGAEDVDHPEGLEHVLSVTSAAVVKDHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFU5</accession>
    <name>C5DFU5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0D18018p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0D18018g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR23050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553488.1">
        <property type="nucleotide sequence ID" value="XM_002553442.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFU5"/>
    <dbReference type="GeneID" id="8295740"/>
    <dbReference type="KEGG" id="lth:KLTH0D18018g"/>
    <dbReference type="eggNOG" id="ENOG502SEHY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_193142_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFU5"/>
    <dbReference type="OMA" id="CHTLIAR"/>
    <dbReference type="OrthoDB" id="4068933at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="85" mass="9603" checksum="41C489F1EFD65749" modified="2009-07-28" version="1">MALHNYIYLKHKSHDPECHTLIARDHDNRPLPQERALPQASRAATATSELPAEAENRTKSKAQTVKTDKEECCGDCQGKCDTLQW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DG46</accession>
    <name>C5DG46_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0D02332p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0D02332g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552826.1">
        <property type="nucleotide sequence ID" value="XM_002552780.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DG46"/>
    <dbReference type="FunCoup" id="C5DG46">
        <property type="interactions" value="135"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DG46"/>
    <dbReference type="GeneID" id="8295046"/>
    <dbReference type="KEGG" id="lth:KLTH0D02332g"/>
    <dbReference type="eggNOG" id="KOG1426">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005210_0_0_1"/>
    <dbReference type="InParanoid" id="C5DG46"/>
    <dbReference type="OMA" id="GWGANTK"/>
    <dbReference type="OrthoDB" id="5370059at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051210">
        <property type="entry name" value="Ub_ligase/GEF_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22870:SF155">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HERC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22870">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="323" mass="34692" checksum="B43A475EFFD576D8" modified="2009-07-28" version="1">MITPQSTFKSLSKPLKVTGGGNFTAILLENGELYVAGECTLVSREKTAGWCQIEGLFRDVACGWSHIIAVTCEQEVVTAGIGVKGELGLGSKNKTNQFERVLAVQSPGAQVFACFYNTYVQDGGKLYGWGSNTKCQVLSPKSKAVSCPALVYEGKISNVCVGKNSVCFVGDGLIRAQGAIAKYLPQMRSEPVLGEKLELRSMWTSITAFDHRNFRFFGPAGQMQDAITFCKQPPAYAVSCWGTGSEHGVMCCEDRVYCWGWGEHGNCGPTNATTAASLTSGINDQSNTKSGVNMVYITSHQEEVVSCFGGCATTWICIKKTGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>C5DG71</accession>
    <name>C5DG71_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0D02882p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0D02882g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552851.1">
        <property type="nucleotide sequence ID" value="XM_002552805.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DG71"/>
    <dbReference type="FunCoup" id="C5DG71">
        <property type="interactions" value="37"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DG71"/>
    <dbReference type="GeneID" id="8295071"/>
    <dbReference type="KEGG" id="lth:KLTH0D02882g"/>
    <dbReference type="eggNOG" id="ENOG502QTI4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065195_0_0_1"/>
    <dbReference type="InParanoid" id="C5DG71"/>
    <dbReference type="OMA" id="EYKRTIV"/>
    <dbReference type="OrthoDB" id="5599171at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071595">
        <property type="term" value="C:Nem1-Spo7 phosphatase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006998">
        <property type="term" value="P:nuclear envelope organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005605">
        <property type="entry name" value="Spo7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28249">
        <property type="entry name" value="SPORULATION-SPECIFIC PROTEIN SPO7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28249:SF1">
        <property type="entry name" value="SPORULATION-SPECIFIC PROTEIN SPO7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03907">
        <property type="entry name" value="Spo7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="121"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="55"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR22413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="313" mass="35873" checksum="28BF0EAFAC74B5B2" modified="2009-07-28" version="1">MDHSLELSTDKIGKLDEPQIEPGNALGNAFPVVKIEEYQDNSDETPIKVRRRGGKAQSKPSSPQPASLNISQGATRRRRSSSRTSSKNLKSTTDISPASMIFRNLLILEDDLRRQAREQKILKWQFTLFLSALVGIAAAAFYELYFSQESVKGIYKVMLEFVLIFILVTVVLFHLSGEYRRTIVIPRKFFTSTNKGIRQFNVKLVKVKSSIPDLCIDLVRWVCRKSAVVNLLLSRKCLPARIAEDSWLLRFWTSVALRSQPRIGAVDVKLILNPRAFSAEIREGWEIYRDEFWAREGARRRKKVNETSPRKDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>C5DG78</accession>
    <name>C5DG78_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">KLTH0D03036p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0D03036g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552858.1">
        <property type="nucleotide sequence ID" value="XM_002552812.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DG78"/>
    <dbReference type="FunCoup" id="C5DG78">
        <property type="interactions" value="1343"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DG78"/>
    <dbReference type="GeneID" id="8295078"/>
    <dbReference type="KEGG" id="lth:KLTH0D03036g"/>
    <dbReference type="eggNOG" id="KOG2294">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003924_0_0_1"/>
    <dbReference type="InParanoid" id="C5DG78"/>
    <dbReference type="OMA" id="IAVQRPM"/>
    <dbReference type="OrthoDB" id="5954824at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060962">
        <property type="term" value="P:regulation of ribosomal protein gene transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00059">
        <property type="entry name" value="FH_FOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22701">
        <property type="entry name" value="FHA_FKH1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.20:FF:000039">
        <property type="entry name" value="Forkhead transcription factor Fkh1/2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045178">
        <property type="entry name" value="Fhl1/FHA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030456">
        <property type="entry name" value="TF_fork_head_CS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21712">
        <property type="entry name" value="PRE-RRNA-PROCESSING PROTEIN FHL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21712:SF29">
        <property type="entry name" value="PRE-RRNA-PROCESSING PROTEIN FHL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00658">
        <property type="entry name" value="FORK_HEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="FHA" evidence="9">
        <location>
            <begin position="296"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="Fork-head" evidence="10">
        <location>
            <begin position="473"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="6">
        <location>
            <begin position="473"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="147"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="418"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="718"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="547"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="20"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="60"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="156"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="425"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="438"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="736"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="764"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="826"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="838"/>
            <end position="852"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR22420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="858" mass="93227" checksum="1C03B2B82C66A2EC" modified="2009-07-28" version="1">METSVDALTPSGPAAEDPAEERHNSDEHRSPKDEPENFGEPFAKKPSRSNSQVAADEAAAEAKRLIDDPNLKLEIPDSLDHEHFSKLLELDSKNDDDLFSSTDLLEHEIAEHPEKLANDIAETVPTDDATQHLHSLENLESLGKLSEEAGGDVHSNPGTESPSKIKNENQSPSVLVDPVLADDRAIHPDIYNSDPLSTGAGTREDTPASILEDKHELLDDQKQDASTKLGYVSLQPPLPENAVNKIEITQPIEPNSELPMTEFHDGANSETEQSKISAYARLDFQSFTFYVQTLQVIIGRRSENDFSHKVDVNLGPSKSISRRHAQIFYNFGTGRFELSIMGKNGAFVDDTFVERGITVQLKNKAKVQIGQIPFQFVLPDQEGKEKKAIEPVDAISLKTALNNNNKLAEISRSVTPIPIPSGESTELKDEELKDQPAKKKTPPKKKEKKEPKAPKPPKKIYTLEEIPPEYRTKPTGSYSNLITACLRKFSSPKGMSLSGIYAGIRELFPYYKYCPDGWQSSVRHNLSLNKSFRKVSKEGKGWLWGLNEEYIAEREKQKKKQAEAAAEKAKAAQLKLEQQQQQKAKKAAESGSMIKSVASIPARPKVKQPNISQTLAANRADRKATADANQRTMKYLQEQLMILTKDRKGLDKHVMASILTQALAMTINQVTQAAKSKGITGNPLTALIDKNPQHLNLILAAAVNAATVKVTNGSVKQLVSMPPPTVTAPQERRAASPSTSNIQPTSGGTSNLVASANPGPAATVKSSESFDPTSLSKFFQPKQPSRVPTPSPARSVLPLKRASSETESSSDDESTSGDESGSADSDASDGSDSDDDSSSSSGGSSKGSSSDEGSSDGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DG80</accession>
    <name>C5DG80_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0D03080p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0D03080g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552860.1">
        <property type="nucleotide sequence ID" value="XM_002552814.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DG80"/>
    <dbReference type="FunCoup" id="C5DG80">
        <property type="interactions" value="596"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DG80"/>
    <dbReference type="GeneID" id="8295080"/>
    <dbReference type="KEGG" id="lth:KLTH0D03080g"/>
    <dbReference type="eggNOG" id="KOG0661">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_176_1_1"/>
    <dbReference type="InParanoid" id="C5DG80"/>
    <dbReference type="OMA" id="MPAHKNL"/>
    <dbReference type="OrthoDB" id="2158884at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07830">
        <property type="entry name" value="STKc_MAK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000624">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="36"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="485"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="490"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR22422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="640" mass="72003" checksum="63D5AC145A227500" modified="2009-07-28" version="1">MGYLGRKHGLKSSTNVPPDVNNPPFYIPVRPIANRYTLMQELGNGSFGSVTLAKLAGSEGADKDARGQHDHKRLAASTRQHYHNTLMDDAVVPSIEQENYHNKQRGVLAIKTMMTRLPTLNDYTRVREVKFILSMPAHKNLVQIWELFIDDINYQLHIVMECMEQNLYQLMRARRKRVFSLPSLKSILSQILAGVRHIHAHNFFHRDLKPENILISPSSHYFSKEWILEGHYSDNYVVKIADYGLARHITNKSPYTAYVSTRWYRSPEILLRKGLYSRPLDIWAFGCVVVEVATFRPLFPGSDEMDQIWKILEVLGTPHTMPESTLSGYHPHGGLWEKAQVLASRLNLKFPYVEGVSIESIMDNPHLQPLCDVVKACLVWDPKKRATVDEIFCMPYFEEAKPWAEPKGLNDSNTDNCPLNDENLNKGLGLRHYWSGGLNRGSRHPHGENASLAPIEVDSNGAKIRGPKETEISFRQFLKESLLPLPMSKETSRGSDGNRLHNEAGPEDVADLPLSEDSVANISPVSSLNDAENVGVLEPPEVDDSFAAFFVSKSAEFEYPAGAEVAAGNLEPSYIHYGDSTDDRIIPHHRCNVLDDMSVDSSGSNRVPRTFAVPRISEECNVSAQASHNHSFSHTNSLTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-04-02" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DG83</accession>
    <name>C5DG83_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0D03146p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0D03146g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552863.1">
        <property type="nucleotide sequence ID" value="XM_002552817.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DG83"/>
    <dbReference type="STRING" id="559295.C5DG83"/>
    <dbReference type="GeneID" id="8295083"/>
    <dbReference type="KEGG" id="lth:KLTH0D03146g"/>
    <dbReference type="eggNOG" id="KOG1492">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_615494_0_0_1"/>
    <dbReference type="InParanoid" id="C5DG83"/>
    <dbReference type="OMA" id="KFVHDPR"/>
    <dbReference type="OrthoDB" id="410307at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46156">
        <property type="entry name" value="CCCH ZINGC FINGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46156:SF1">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="195"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="221"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="295"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="195"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="221"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="295"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="392"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="414"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="434"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR22425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="445" mass="50609" checksum="07FB847A8F68B911" modified="2009-07-28" version="1">MSSDIGKTELLAKLERLDQEINDYFDLTLDKARLRYKRVGRGNKVKVVDKIQEKRVAVQKWRDSVKTDYETAVTKFKRAWDLLQNNPELDLCAVTDNLGNSRIFQVHKLGAVLENVSLTNLEVSALKQHDTEFPRTLASMGASKEDYDGFLEWQVNILGRSATLYEQLPKVYWNSHKYQWSKNSYRICATAKNLGSYATYCKFFSKLGICTNANCKFVHDPRNIAACKDFLASDNCKYGSKCRLSHKLGNEYVTPHCKDFAQRSCKFERGAEQWTQSREQDSAVCCRYIHSTIVNSSYPVCRQFAHMGFCYRGLHCKFPHYLECPDSNYTSTCFLNHCKFPHIERKVLPDERAVALIDIPSSCLLLPPAREKDHRAPNSSWYGLRTKQQSINIKDGPGEKFTTEAVPALQLEPGSSTDDVSSSEVSSTGSSDISSDELDADFIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DG86</accession>
    <name>C5DG86_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0D03212p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0D03212g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552866.1">
        <property type="nucleotide sequence ID" value="XM_002552820.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DG86"/>
    <dbReference type="FunCoup" id="C5DG86">
        <property type="interactions" value="159"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DG86"/>
    <dbReference type="GeneID" id="8295086"/>
    <dbReference type="KEGG" id="lth:KLTH0D03212g"/>
    <dbReference type="eggNOG" id="KOG2354">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072845_1_0_1"/>
    <dbReference type="InParanoid" id="C5DG86"/>
    <dbReference type="OMA" id="NCHASIK"/>
    <dbReference type="OrthoDB" id="340681at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006886">
        <property type="entry name" value="RNA_pol_III_Rpc5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12069:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12069">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES III 80 KDA POLYPEPTIDE RNA POLYMERASE III SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04801">
        <property type="entry name" value="RPC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR22428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="248" mass="27864" checksum="837FA5FBB06347A7" modified="2009-07-28" version="1">MSEQIENQLFVTEDSGDVVMEDANLQESEEGDPIVREIPINLTNGPFPLHILQYPNKPKKNGNDPVLHPPVSQVRYKSKSSLWELDVPVNTEIFYDKNRAEDEWDSVAQQTLKGVGVQTEGQYVGMVVDEEVYLLPVQAVAQMRPFFKYIDTVAQSKRENEARGAQPSNPAARRAQVVTMSVKSSSEANQPRLGGALLAHKVADEEPPQDLEWIEGTFNQFRESVITEDSRTMMLPLGDENDYLSKAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DG93</accession>
    <name>C5DG93_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase</fullName>
            <ecNumber evidence="4 14">4.2.1.134</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0D03410g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the third of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid elongation. Thereby, it participates to the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a very-long-chain (3R)-3-hydroxyacyl-CoA = a very-long-chain (2E)-enoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:45812"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:83728"/>
            <dbReference type="ChEBI" id="CHEBI:85440"/>
            <dbReference type="EC" id="4.2.1.134"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Endoplasmic reticulum membrane</location>
            <topology evidence="14">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the very long-chain fatty acids dehydratase HACD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.134" evidence="4 14"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552873.1">
        <property type="nucleotide sequence ID" value="XM_002552827.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DG93"/>
    <dbReference type="FunCoup" id="C5DG93">
        <property type="interactions" value="577"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DG93"/>
    <dbReference type="GeneID" id="8295095"/>
    <dbReference type="KEGG" id="lth:KLTH0D03410g"/>
    <dbReference type="eggNOG" id="KOG3187">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034302_6_1_1"/>
    <dbReference type="InParanoid" id="C5DG93"/>
    <dbReference type="OMA" id="WSYILWQ"/>
    <dbReference type="OrthoDB" id="46988at2759"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102158">
        <property type="term" value="F:very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030497">
        <property type="term" value="P:fatty acid elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007482">
        <property type="entry name" value="Tyr_Pase-like_PTPLA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11035">
        <property type="entry name" value="VERY-LONG-CHAIN (3R)-3-HYDROXYACYL-COA DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11035:SF3">
        <property type="entry name" value="VERY-LONG-CHAIN (3R)-3-HYDROXYACYL-COA DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04387">
        <property type="entry name" value="PTPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11 14" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="7 14" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5 14" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9 14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="174"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00036671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR22435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="214" mass="24219" checksum="51216095DA83FCF1" modified="2009-07-28" version="1">MGKVSFVNPLALYNLFSAGLWGYLLYNAIVVYPKIGQPEFFYEIKSTLNAIQCGALIEVFNSLFGIVRAPLLTTFAQVASRLLLTIGVFQYLPEAENAHNKTFVSLVIAWSITEVVRYSFYYFNLTLTCGAPKLLVLLRYNLFWVLYPLGVASELLIIYSALPVGESKYSANVRWALIAAMLTYIPGFPMLFSHMVVQRKKIMRSIRSTTKKEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGB9</accession>
    <name>C5DGB9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0D04048p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0D04048g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF7 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552899.1">
        <property type="nucleotide sequence ID" value="XM_002552853.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGB9"/>
    <dbReference type="FunCoup" id="C5DGB9">
        <property type="interactions" value="572"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGB9"/>
    <dbReference type="GeneID" id="8295124"/>
    <dbReference type="KEGG" id="lth:KLTH0D04048g"/>
    <dbReference type="eggNOG" id="KOG2910">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086201_2_0_1"/>
    <dbReference type="InParanoid" id="C5DGB9"/>
    <dbReference type="OMA" id="RAKQPAM"/>
    <dbReference type="OrthoDB" id="441172at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000815">
        <property type="term" value="C:ESCRT III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005771">
        <property type="term" value="C:multivesicular body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006900">
        <property type="term" value="P:vesicle budding from membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761:SF5">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="163"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="204"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR22461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="214" mass="24219" checksum="09AC1DC10EF6C81D" modified="2009-07-28" version="1">MGQKGSKIEVTKTDKAILQIKLSKDELHRYTRRTEILIGREREKLKALLREDPKNGKKNPRARILLKKIHYQNHLLEQAADQLINLENLVSTVEFKLVEQQFMMGLKQGNEVLTKLNKEFSGAEDLMDSVADQIAYQDEVDQILSSSVVGGFEEELDRELQQLDQEVNGPAHEPAKAEPNLPSTEGLPALSEPEAEEPQQEPQSDGRQREAVLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGX5</accession>
    <name>C5DGX5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DNA polymerase</fullName>
            <ecNumber evidence="12">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">KLTH0D09064g</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="12"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553105.1">
        <property type="nucleotide sequence ID" value="XM_002553059.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DGX5">
        <property type="interactions" value="994"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGX5"/>
    <dbReference type="GeneID" id="8295342"/>
    <dbReference type="KEGG" id="lth:KLTH0D09064g"/>
    <dbReference type="eggNOG" id="KOG0970">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001718_1_0_1"/>
    <dbReference type="InParanoid" id="C5DGX5"/>
    <dbReference type="OMA" id="MTKMNVG"/>
    <dbReference type="OrthoDB" id="6755010at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005658">
        <property type="term" value="C:alpha DNA polymerase:primase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006273">
        <property type="term" value="P:lagging strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902975">
        <property type="term" value="P:mitotic DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05776">
        <property type="entry name" value="DNA_polB_alpha_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05532">
        <property type="entry name" value="POLBc_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.60:FF:000004">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3200.20:FF:000002">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000036">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3200.20">
        <property type="entry name" value="DNA Polymerase alpha, zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.60">
        <property type="entry name" value="DNA polymerase family B, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017964">
        <property type="entry name" value="DNA-dir_DNA_pol_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006133">
        <property type="entry name" value="DNA-dir_DNA_pol_B_exonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006134">
        <property type="entry name" value="DNA-dir_DNA_pol_B_multi_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024647">
        <property type="entry name" value="DNA_pol_a_cat_su_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042087">
        <property type="entry name" value="DNA_pol_B_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038256">
        <property type="entry name" value="Pol_alpha_znc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045846">
        <property type="entry name" value="POLBc_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015088">
        <property type="entry name" value="Znf_DNA-dir_DNA_pol_B_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00592">
        <property type="entry name" value="pol2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45861">
        <property type="entry name" value="DNA POLYMERASE ALPHA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45861:SF1">
        <property type="entry name" value="DNA POLYMERASE ALPHA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12254">
        <property type="entry name" value="DNA_pol_alpha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00136">
        <property type="entry name" value="DNA_pol_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03104">
        <property type="entry name" value="DNA_pol_B_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08996">
        <property type="entry name" value="zf-DNA_Pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00106">
        <property type="entry name" value="DNAPOLB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00116">
        <property type="entry name" value="DNA_POLYMERASE_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0235">DNA replication</keyword>
    <keyword evidence="10 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 12" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 12" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DNA polymerase alpha catalytic subunit N-terminal" evidence="17">
        <location>
            <begin position="10"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B exonuclease" evidence="15">
        <location>
            <begin position="457"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B multifunctional" evidence="14">
        <location>
            <begin position="767"/>
            <end position="1215"/>
        </location>
    </feature>
    <feature type="domain" description="Zinc finger DNA-directed DNA polymerase family B alpha" evidence="16">
        <location>
            <begin position="1257"/>
            <end position="1451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="68"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="195"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="235"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="279"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="800"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="69"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="200"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="279"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF03104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF08996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF12254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAR22667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1453" mass="163592" checksum="B60C9FCB56FAC484" modified="2009-07-28" version="1">MSAKNRIELLKKLQHARQNKSSKSLYEEDETDDKVYDLIDEEEFRSMKRQELLRDDFVVDDDGLGYVDRGVDEWDDRNRDQNYSSEEDDKPRERAAKGSKKIKNDGKGVKSSSGASIGDIIAAQHTKNAKVLSKAPKKFSLDEFDDILQEFDGNEASTAVGGLFGAKSHRVVPKLENNIKREANAAATPPTIKRMKIDEQSFSTPQGGANTSPLAGTKTKANLLDDRDLQAMIDDVQSSPTITKERSADIVKSGGQSSEEDSDEEITFRRRTIRSAAASQRSNVDIRSSVQSSPFVTAPPTPSGSSKLKEENPKSLSFIGKRYSRSDVISDNASSFKFYWLDFAEVDSTLLLFGKISTRENKLISAMIQILNLSRELFFLPREGKTASDVHGEIIPLLMEKYGLENIRAKPEIKKYAFELPGIPHESEYLKVLLPYRTPKNRNVTIPSDLEGDTFHHVFGGNTNIFESFVVQKKVMGPCWLEINNGDFSALSNASHCDIEVQVSQPSSIEPTSDKHVPNLNCASLAVQTIMSSKENKQEIVSITLSQYKNLAQDLPIPEDLAPNDTITLVRPPVGSSFPTGLTHLAKKNLTGNLRLFNNEKALLSCFCAMMKSSDPDVIIGHRLENVALDTILYRCFELKIPTFSCLGRRARKSWPDKFGKNPSMNQFYNRDILSGRLLCDISNEMGQSLTPKCQSWDLPEMYQVTCQKEQRALEINYQNPQYQNDVNSMIMALQENVSNALISAEIAFRIQILSLTKQLTVLAGNAWCQTLGGTRAGRNEYILLHEFERNNYIVPDKETKSMRNQRQQRAREEAGLDGGDEVQVSSKKAKYQGGLVFEPEKGLHKNYVLVMDFNSLYPSIIQEYNICFTTVERDPSNIDELPNVPSSEMAQGVLPRLLASLVQKRREVKKLLKTEPDPHKRTQCDIRQQALKLTANSMYGCLGYVNSRFYAKPLAMLVTNKGREILMNTRQLAESLALTVVYGDTDSVMIDTGSDSYMEAIKIGENFKKLVNERYRLLEIDTDNVFRKLLLHAKKKYAALTVVFDKSGAEKTLLEVKGLDMRRREYCPLSKEVSTHVLETILSDKEPETALQDVYEYLEDIREKVENNTIRVDKYKINTRLSKDPKAYPGGKNMPAVQAALRMREAGRVVKAGSVITFVITKQEGVEDDSSLSPAERARVLNEVMVKNNNLRPDPQFYLEKQIFAPVERLLERIESFDIVRLSESLGLDSRRFINRAGANGELNGSSSGSLQPLESSISDEERFKDVAALTFQCSGCQQRFPFGGIVASKRYQMSYSGLQCSSCQHTFAPLQMSAQLESAIRSHISMYYAGYLACDDPTCGNNTRQVSVFGKRCLNENCMGVMRYRYTDKQLYNQLLYFESLFDVDKNKKDAVKPLYKPDHPDAPEAKIPTSQLLALGEQNRELFQVMSSVVQKYLANCARRFVDMDAIFAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGY5</accession>
    <name>C5DGY5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="15">KLTH0D09284p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0D09284g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Microsome membrane</location>
            <topology evidence="11">Single-pass membrane protein</topology>
            <orientation evidence="11">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12 13">Belongs to the cytochrome b5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553115.1">
        <property type="nucleotide sequence ID" value="XM_002553069.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGY5"/>
    <dbReference type="FunCoup" id="C5DGY5">
        <property type="interactions" value="764"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGY5"/>
    <dbReference type="GeneID" id="8295352"/>
    <dbReference type="KEGG" id="lth:KLTH0D09284g"/>
    <dbReference type="eggNOG" id="KOG0537">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102602_3_2_1"/>
    <dbReference type="InParanoid" id="C5DGY5"/>
    <dbReference type="OMA" id="WIVIHND"/>
    <dbReference type="OrthoDB" id="260519at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016126">
        <property type="term" value="P:sterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.120.10:FF:000002">
        <property type="entry name" value="Cytochrome b5 type B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050668">
        <property type="entry name" value="Cytochrome_b5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19359">
        <property type="entry name" value="CYTOCHROME B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19359:SF150">
        <property type="entry name" value="CYTOCHROME B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00363">
        <property type="entry name" value="CYTOCHROMEB5"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3 13" id="KW-0349">Heme</keyword>
    <keyword evidence="9 13" id="KW-0408">Iron</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0492">Microsome</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="102"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="14">
        <location>
            <begin position="2"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR22677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="123" mass="13259" checksum="D6555CF582C9636E" modified="2009-07-28" version="1">MAKLYSYKEIAEHNTENDLWMIIDGKVYDCTKFMDEHPGGEEVLLDLGGQDATGPFADIGHSDDAVKMLEDLYVGDVDKDSEPIAVVKGDPASTTTGGEGNGVMILAIAAVALAVVYFYLNQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DH14</accession>
    <name>C5DH14_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0E00550p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0E00550g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553512.1">
        <property type="nucleotide sequence ID" value="XM_002553466.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DH14"/>
    <dbReference type="FunCoup" id="C5DH14">
        <property type="interactions" value="155"/>
    </dbReference>
    <dbReference type="GeneID" id="8291649"/>
    <dbReference type="KEGG" id="lth:KLTH0E00550g"/>
    <dbReference type="eggNOG" id="KOG1286">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007946_12_2_1"/>
    <dbReference type="InParanoid" id="C5DH14"/>
    <dbReference type="OMA" id="FIVWTGI"/>
    <dbReference type="OrthoDB" id="3900342at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF26">
        <property type="entry name" value="GENERAL AMINO ACID PERMEASE AGP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="75"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="113"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="155"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="185"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="226"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="260"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="316"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="362"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="388"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="430"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="467"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="6">
        <location>
            <begin position="50"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR23075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="523" mass="56994" checksum="2AFC8209701F9040" modified="2009-07-28" version="1">MPKENDKTHGSASIVSAESVDSTGYVVNAKGEVLLSNGLKPGLKNRMINLMAICGIIGPGVFVGMGSMLKSSGGAGLLAGFAIVGVLCMYMMLSVGELNSTFDFNFCKHGTRFVSPGFGAALALAYVVLWITNLISEYTSLTSICETYTDKVPTYGWFLIFWAFFTLFQLLDVTAYGESEYILGFLKLGFITAFYVFAIVYASGGVPNNKPDNPFGKHPLVDGFKGIANSFVFAGVFLSGIESVSIFASESRNPAKAIPVAVRSTVIRIFYVYFGISIAYGITVSPTDAALSDPHKSMKAPMTIALTNAGWVNGKYLITTIILVTCISSINSAIFLASRSLFTWSEMGMGPKIFTKTNKKGVPYVAVHFCHLFGFLSILSYSAGSSVAYNYVVNVAGVCAFIVWTSISFIHLRFRRGWVKQSKPLSDLGFVAPLFPWINIVGITLGTILVLVQGWSSFCPWDKKAFIDGYIMLPVFFLVWGLYDLVLLKTKIIKSSEMDFESDRKLDLDALKKETSVVIVEGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5DH40</accession>
    <name>C5DH40_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0E01144p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0E01144g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553538.1">
        <property type="nucleotide sequence ID" value="XM_002553492.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DH40"/>
    <dbReference type="GeneID" id="8291676"/>
    <dbReference type="KEGG" id="lth:KLTH0E01144g"/>
    <dbReference type="eggNOG" id="KOG1289">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004495_0_3_1"/>
    <dbReference type="InParanoid" id="C5DH40"/>
    <dbReference type="OMA" id="QFWAFAR"/>
    <dbReference type="OrthoDB" id="4476201at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649">
        <property type="entry name" value="AMINO-ACID PERMEASE BAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649:SF6">
        <property type="entry name" value="GABA-SPECIFIC PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="102"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="152"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="223"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="304"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="361"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="410"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="438"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="480"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="511"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR23101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="572" mass="62311" checksum="3AF5808F56395F97" modified="2009-07-28" version="1">MSASSSNKGTIKNRKSHVDVEVGQMRELGTITSAHGAPIRFVDARSATSDQQVLAELGYKEELKREFSSIQIFGVAFSIMGLLPSIASTLDGGLSAGPVGLGWGWFIAGGLILTIGIALGELSSAMPTAGAVYVSCYQWAPQRVRKCISYSVGFLDTLSLSASVCSIVYGLAQQILSAVLVSNPDFNATDPITYGVFAACIVCMAILTSLPSRITSKLQTISIISNCFLIVLFFIAIPIGVSRSDTLNRSFNGGDFIFGQVDNRSTWNKGWNWCIAGLMPAIWTIGAYDSCLHMAEEARDAVRAVPFGIVGSITVCWILGWLVCIVILACMNPDIDSVLDSQFGQAMTQIIYDALGKRWTLGFLSIFIICQFLMGASTVVANSRQFWAFARDDGFPFSNFFKKVDGLTGVPVRAVWGSSALSLALGCLCLVGETASGALFSLSVAGMYMALIFPITLRLTYGKKDFRPGPFYLGDFWSPIINWISVFFQAFVIIMMMFPSDSTVTPTTMNYTVVIGPGFWVLSLIYYFVWQRKFYKGPKSNLTDEEFQEYVGDDVIDAIMSNNEADYKPGDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DH57</accession>
    <name>C5DH57_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0E01518p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0E01518g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required for vesicular transport between the endoplasmic reticulum and the Golgi apparatus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the SNAP family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553555.1">
        <property type="nucleotide sequence ID" value="XM_002553509.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DH57"/>
    <dbReference type="FunCoup" id="C5DH57">
        <property type="interactions" value="947"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DH57"/>
    <dbReference type="GeneID" id="8291693"/>
    <dbReference type="KEGG" id="lth:KLTH0E01518g"/>
    <dbReference type="eggNOG" id="KOG1586">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046329_0_2_1"/>
    <dbReference type="InParanoid" id="C5DH57"/>
    <dbReference type="OMA" id="WSVKEYL"/>
    <dbReference type="OrthoDB" id="9984275at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005483">
        <property type="term" value="F:soluble NSF attachment protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035494">
        <property type="term" value="P:SNARE complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15832">
        <property type="entry name" value="SNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000049">
        <property type="entry name" value="Alpha-soluble NSF attachment protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000744">
        <property type="entry name" value="NSF_attach"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13768:SF8">
        <property type="entry name" value="ALPHA-SOLUBLE NSF ATTACHMENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13768">
        <property type="entry name" value="SOLUBLE NSF ATTACHMENT PROTEIN SNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14938">
        <property type="entry name" value="SNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00448">
        <property type="entry name" value="NSFATTACHMNT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR23118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="292" mass="32429" checksum="706C348B8A4CD420" modified="2009-07-28" version="1">MSDPQALVQKARKKAQPSSGFFKLFGASDSYKFEEAADLFVEAANLYKLRRELEPAGELFLEAASCQIKAGNDDEAGNTYVEAFKAFKAGASPSKACDALNRAIEIFTRRGQFRRGANFKFELAEILESDLHDFPHAIDAYEAAADWYAQDQALALANKAYLKCADLKALDARYIEACDVYRKVIANSVGNKLSQWSLKDYYLKTALCYLAADDQVAARRTESEALAEDSNFAGSREHDLLAALLEACSEGDAEKLSAKVYEFDRFSKLDKWKTTILLKIKDTIATADDDLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHR4</accession>
    <name>C5DHR4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cytochrome c oxidase subunit</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Cytochrome c oxidase polypeptide VIa</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0E06512g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the cytochrome c oxidase subunit 6A family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553762.1">
        <property type="nucleotide sequence ID" value="XM_002553716.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHR4"/>
    <dbReference type="FunCoup" id="C5DHR4">
        <property type="interactions" value="301"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHR4"/>
    <dbReference type="GeneID" id="8291917"/>
    <dbReference type="KEGG" id="lth:KLTH0E06512g"/>
    <dbReference type="eggNOG" id="KOG3469">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_122515_0_1_1"/>
    <dbReference type="InParanoid" id="C5DHR4"/>
    <dbReference type="OMA" id="KLPWMVD"/>
    <dbReference type="OrthoDB" id="5947505at2759"/>
    <dbReference type="UniPathway" id="UPA00705"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.95.10:FF:000001">
        <property type="entry name" value="Cytochrome c oxidase subunit 6A, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.95.10">
        <property type="entry name" value="Cytochrome c oxidase, subunit VIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001349">
        <property type="entry name" value="Cyt_c_oxidase_su6a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018507">
        <property type="entry name" value="Cyt_c_oxidase_su6a_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036418">
        <property type="entry name" value="Cyt_c_oxidase_su6a_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11504">
        <property type="entry name" value="CYTOCHROME C OXIDASE POLYPEPTIDE VIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11504:SF0">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02046">
        <property type="entry name" value="COX6A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000277">
        <property type="entry name" value="COX6A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81411">
        <property type="entry name" value="Mitochondrial cytochrome c oxidase subunit VIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01329">
        <property type="entry name" value="COX6A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 12" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="49"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR23325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="127" mass="14416" checksum="0956FAC7CDBA6E28" modified="2009-07-28" version="1">MLRQAIRRASSLPPHALKPAYGPGDAAAAKAFKEMAQNTKHHAKETSGLWLKISFFVAAPAIALAAVNTYFVEAEHAEHRKHLKHVPDSEWPAQYDYQNIRSKPFFWGDGDKTLFWNPIVNRHVQQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHS8</accession>
    <name>C5DHS8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0E06820p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0E06820g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553776.1">
        <property type="nucleotide sequence ID" value="XM_002553730.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHS8"/>
    <dbReference type="FunCoup" id="C5DHS8">
        <property type="interactions" value="1204"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHS8"/>
    <dbReference type="GeneID" id="8291931"/>
    <dbReference type="KEGG" id="lth:KLTH0E06820g"/>
    <dbReference type="eggNOG" id="KOG3117">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065858_0_0_1"/>
    <dbReference type="InParanoid" id="C5DHS8"/>
    <dbReference type="OMA" id="RHTKSER"/>
    <dbReference type="OrthoDB" id="203440at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13237:SF9">
        <property type="entry name" value="NEUROGUIDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13237">
        <property type="entry name" value="SOMETHING ABOUT SILENCING PROTEIN 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="315"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="207"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="363"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="372" mass="40546" checksum="CF62CF2B76B621AE" modified="2009-07-28" version="1">MSELGDVLREINASLAATSESLDSARRQEASAAHATGAARELSGGRELEKVSLLTLKNASLLAYVNSLMVVVGEKLGRTDASAKSGRAQSVEHRVVLERGVRPLEKKLAYQLDKLVRAYTRMEGEYEAAEKRALAAQDEAAAGRDSGDGASGDEAARGGRSRANADDDADEGSNSDDSDSDSETEAQAFRPNAAGLIGRSARDKHTAGAAPAAGTDDAPAEAYKPPRISAALPPQHHFEDKFDAQQHRDRSGKNRMQAMEEYVREVSEQPDWEASVGANIVDHGKGGVKTLRDTQREQRIKDYEEDNFTRVNVKGNKAERRRAKQKERAARANMLGGEDFSIFNSKRRLEDSTSRSAAKRPRSAWDRAKNRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHX2</accession>
    <name>C5DHX2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15 18">Delta(24(24(1)))-sterol reductase</fullName>
            <ecNumber evidence="15 18">1.3.1.71</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">C-24(28) sterol reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Sterol Delta(24(28))-reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ordered locus">KLTH0E07832g</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
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            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="20">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
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        <reaction evidence="16">
            <text>ergosterol + NADP(+) = ergosta-5,7,22,24(28)-tetraen-3beta-ol + NADPH + H(+)</text>
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            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
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            <dbReference type="Rhea" id="RHEA:18503"/>
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    </comment>
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        <text evidence="17">Steroid metabolism; ergosterol biosynthesis; ergosterol from zymosterol: step 5/5.</text>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <accession>C5DI23</accession>
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        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
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                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
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                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
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                <person name="Fairhead C."/>
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                <person name="Leh V."/>
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        </source>
    </evidence>
    <sequence length="928" mass="102418" checksum="699DF88BC383E28B" modified="2009-07-28" version="1">MNTGPQNLVRKEPNRAQLRSQLLNNDLGTIDLAQERPINQAAPSQYPMAPAYNNAYQPQQPQYGQPPFQPGRASYVQQGYQAADQRRPSLQSQSAGGKRSLQSNAISGLFKMRNGRSKAKDSDEEDDTFMTDGDSSILTFSDISSLRNNGGHKYGFGGAMDDTSPIIPTLITKGHEKMNNIEYRKHLAAQKKMTMNALSKQNKPMHQVPPQGGPRTMSLQHSYNPYNAQAPPRDNFNQGPPYARANSMMSGPPPQMRYPNARGTPPPPGAGAPRTMSLTTNRRNMANPQGGFRPQAAAFAARPTSPYIGGQPPMPYQGPRPSNMMVAPGGGYPIQDIKPAAPLPNENVTPHSSSSLVSSNSLASHRPSSQTPDSQNIDDPSTVSNLLSGSAQKLPRESPLKSEVNNTGKLNIIKLSDPQQALRDKEQFLQERERLIAEKEEELRVKEREAQEQKTRSATVEKQPPLNTYNTVSEQSPKFKLSRQRSDPQVTQPNSGRIGRRSQVQSMATFESSLSNDSPVKKKDDKTGLYMLDNATEGNAYFTASDLLNEEASRLHENLSDVTITRDETKPEKTASSSAGALNKGQDESEGKTAGMGKARKFFKRLSSSGAKSTNSVTNSRKSSMSSVRRVSSSESVIADRRASPEGKKSWSVKSDNTKRRSFHSLFSNSSIGLNSTEPLKPHTGLTNIEEKRGRDDADASKEQGVYQESAGPTSPVKPLRFDNANKRSDSNSEKSEDEADRTLEANSHDEENVGNEFVFDNSVSRPYKQTYSSESELEAASELNPKSKLRTISISGSQTSILNEQGTLMNEINLLSKELAESIARESRLEQQLAGKGKDSKENEALSITDFETELRKKSTKIVELIQQLNDERLRRYIAEEQIMLQEHGAKPSSVDLIHKIQIQNQQIVAKDEEISKLKEQLQRTQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DI29</accession>
    <name>C5DI29_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0E09240p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0E09240g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553877.1">
        <property type="nucleotide sequence ID" value="XM_002553831.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DI29"/>
    <dbReference type="FunCoup" id="C5DI29">
        <property type="interactions" value="421"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DI29"/>
    <dbReference type="GeneID" id="8292040"/>
    <dbReference type="KEGG" id="lth:KLTH0E09240g"/>
    <dbReference type="eggNOG" id="ENOG502QQT5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043834_0_0_1"/>
    <dbReference type="InParanoid" id="C5DI29"/>
    <dbReference type="OMA" id="PNWYSLP"/>
    <dbReference type="OrthoDB" id="5354116at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="303"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="344"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="304"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="348"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="516" mass="58454" checksum="719FF14F0569AA3A" modified="2009-07-28" version="1">MDHTVVFNNLQSVLQAASSKCKVIDEKFPSKFFEKDPARIFESYKKFLKPKLDGEGNVKNKDNLPVTTIAEKFSKNEYVKRPGAFYRLHHDIKLVCTLLIHFYPQGTRTYQMVDKFYKFATELLLRECYRYGISMVEESEEQYSDEKTLFNKQVSRDFIKISVGYSVPYAESYYISSGGLDLFSSTISKSQLDHRVTEPPNSSFVSNKIVPQAGNEIAPKLGFVAANVSNIPDPTLPPSEMMTKFLHPNWYALPTTVWLEYGDFKSWAPSFNESGTVLDAARRGTIWLEKTGYTSLWKLKNKHKDTLTREDSEEASNKETNNESTESVSAGTEALKHNDIAQPIGVSKESSPQKNVESEQNVGNTSNGASSIEIDEHQADDKETGENNPVSSPISSGSLKIKLENLYEWSPGNNISDEEIQVITEGNAHKLVTQTLLELKEMKRKRVKSKKVSKPAAKEMELYYRAQRLLREVMLAKKVESVPQITAKSFPVLQANYMGSVPVVRTIQTRKKKYKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5DI31</accession>
    <name>C5DI31_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0E09284p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0E09284g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553879.1">
        <property type="nucleotide sequence ID" value="XM_002553833.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DI31"/>
    <dbReference type="FunCoup" id="C5DI31">
        <property type="interactions" value="122"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DI31"/>
    <dbReference type="GeneID" id="8292042"/>
    <dbReference type="KEGG" id="lth:KLTH0E09284g"/>
    <dbReference type="eggNOG" id="ENOG502QQ5B">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002415_0_0_1"/>
    <dbReference type="InParanoid" id="C5DI31"/>
    <dbReference type="OMA" id="DRSNCKR"/>
    <dbReference type="OrthoDB" id="5529162at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070762">
        <property type="term" value="C:nuclear pore transmembrane ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006999">
        <property type="term" value="P:nuclear pore organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056541">
        <property type="entry name" value="Ig-like_POM152"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056542">
        <property type="entry name" value="Ig-like_POM152_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056543">
        <property type="entry name" value="Ig-like_POM152_9th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056544">
        <property type="entry name" value="Ig_POM152"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037701">
        <property type="entry name" value="Pom152"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056540">
        <property type="entry name" value="TMD_POM152"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28206">
        <property type="entry name" value="NUCLEOPORIN POM152"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28206:SF1">
        <property type="entry name" value="NUCLEOPORIN POM152"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24312">
        <property type="entry name" value="Ig-like_POM152"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24519">
        <property type="entry name" value="Ig-like_Pom152_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24527">
        <property type="entry name" value="Ig-like_Pom152_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23664">
        <property type="entry name" value="Ig_Pom152"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24097">
        <property type="entry name" value="TMD_POM152"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleoporin POM152 N-terminal transmembrane" evidence="2">
        <location>
            <begin position="53"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin POM152 first Ig-like" evidence="4">
        <location>
            <begin position="197"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin POM152 Ig-like" evidence="3">
        <location>
            <begin position="469"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin POM152 immunoglobulin-like" evidence="1">
        <location>
            <begin position="545"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin POM152 Ig-like" evidence="3">
        <location>
            <begin position="756"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin POM152 immunoglobulin-like" evidence="1">
        <location>
            <begin position="883"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin POM152 ninth Ig-like" evidence="5">
        <location>
            <begin position="1080"/>
            <end position="1152"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF24527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR23442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1269" mass="142060" checksum="410B2ACBB31948CE" modified="2009-07-28" version="1">MDFRHRFNDEKPSSARLRERSSSFARTAWHDSTHEMSASRKDDLPLISTEVLDIPSQRALVVAVFAIIQAYKLYDLILLKSGLPVAGVLLTSSRFNFISKYLVIDSLFLYFLPGLKIPKLTFRPVAVLLQIMAVTATTVFLSNEQNFPLLTFLVSAWTKFNAKELSLTGGSVSQRKLVDPASHFKGAHTIKILPENTAMLNPFHDSFCINLDGTSVDDIHIPLRINSTSELDFVQLEYHSLDSPAKELLNFTKKQLVEVPRSHELFQRDRASHHNSIRYFLLPVEEIGLYKVKQVIDSKKFHLRLYKSEVIIPHCPWGVIAGLGNFDRCVGDSNKVSIEVHGVPPMELEYTRSLNGESSKFVDSSLQPEYFDSPLMFNQKAYTAENLADLKWARSYPVTINLEALLKDDGVHSYTLQRVVDGFGNKMDFSGLSSSLLEKYGLSYSFKVHDLPKATLDSKFDHNSPTKRSLEIKFESSRDWDDAVPYKAAIEVINDSEERSILEYELKKPVTEIPIDQPGTYRLESVGSKFCSGIVVDKSNILVTQPIPPRLEVKSTPILDQCVGQVGLNFDLTFTGVPPFYYRTKIYKIEGKERKLHDAKKFTSQGTRNQFHYSPSSEGNYEIVFDQISNTLFTKAIPLSPVEDYTFKTSMRVKPDAAISSHYATQLCLGSRSKVPVAFKGEPPFSLEYDILETSTNKRVTYSLNDINSFNYDLQTPVFDVGGDYILSLVSVKDTSGCLVGLSSSDARIEVRRDVPSAGFNSFENHDVMLIKEGAFAELPLSLSGVAPFTVSYQHFDSKGEHMGTYDATFMSSYKAGLKVEKEGTYKLMSVKDRSCLGKISESKEFSVAFLEKPGFKVLEHNKLSKHDDMVFAKDDVCENFEETVDLVLSGSAPFVLSYELMSPSGQIASRSIQVATKFASIKLPNDRSGQYTLTVKGVSDSHYTEKDLAKIGFTTLDVVVKQHVRPLPAIGFAEPGKTFRTCSADVDNLSGLEKIALHVKSGRAPFSVSFSIYHESTSKTDYVTIDNVTKKNFDFSKLFKGLKLGTHIVTMGKVVDANGCTSESGSDKNHILISITDVPRISLAEPNMEFCVGDYVAYQLSGLAPFNIKYEFNGVPLKSKERTSQFVRLASEAGIISVLSLQDSASQCLVNFTRPGMEREFEALSLVVHPIPSVTVSRGDYIVEDIHEGDQAEIIFSFEGTPPFSLTYVRTEEVDDKRGKKRPQIVETHKVEDIYSYEYRTFTSLQGRYEAIAISDAYCFAKNNAYFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DI42</accession>
    <name>C5DI42_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0E09548p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0E09548g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553890.1">
        <property type="nucleotide sequence ID" value="XM_002553844.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DI42"/>
    <dbReference type="STRING" id="559295.C5DI42"/>
    <dbReference type="GeneID" id="8292054"/>
    <dbReference type="KEGG" id="lth:KLTH0E09548g"/>
    <dbReference type="eggNOG" id="KOG1601">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_607004_0_0_1"/>
    <dbReference type="InParanoid" id="C5DI42"/>
    <dbReference type="OrthoDB" id="2162994at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051140">
        <property type="entry name" value="GATA_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45658">
        <property type="entry name" value="GATA TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="6">
        <location>
            <begin position="407"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="77"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="296"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="81"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="98"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="335"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="352"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR23453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="503" mass="57014" checksum="CDFF11C3302DBD32" modified="2009-07-28" version="1">MSVATEKKSIYHTNEKAPEYASVSRPQFHAGSVCLTAYSPTQDQIVREAKCMGKTSRQPTRQSVPSLASLLRVSPSPLDLHAGHKEASTPDSERTISRRSSFSNTASRDQSMKARAINWPTEFQQEERAVDSRAPAYQYVSSQRPKLPTHGRKHSLDSLMTAAQTLEREVQKSMLSIENLDRTYQAKILGIIELKNAISGNLENWPITKGALHIDRHQSCLVDSLPLRSIEEMLQSSKKLVAEVTDLLAIKTRATPYTETAAPSNQSYTHQPQQQLILPSPAEMLSCRQLGTDHHASRKLSFSKQPLKASATRVGDNKVWHQSHMPHFDQGLNSEKGETHSSKEHSTHDQRPTQTAVHLTSKSPQRLRDKEQQAFRIEKPERLTTQIFALTKPSFDIPLESCMPRDLPETMECVHCSRKDTPEWRRGPYGNRTVCNACGLFYGKLVRRFGAHRANIMMHYRRRTAPEDRRVPNAFFVPESFLKELHQDVTFEEVPVRTSLLSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>C5DI78</accession>
    <name>C5DI78_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0E10362p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0E10362g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553926.1">
        <property type="nucleotide sequence ID" value="XM_002553880.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DI78"/>
    <dbReference type="STRING" id="559295.C5DI78"/>
    <dbReference type="GeneID" id="8292091"/>
    <dbReference type="KEGG" id="lth:KLTH0E10362g"/>
    <dbReference type="eggNOG" id="ENOG502S5B4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_858075_0_0_1"/>
    <dbReference type="InParanoid" id="C5DI78"/>
    <dbReference type="OrthoDB" id="4036644at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="125"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="314"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="332"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR23489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="341" mass="36372" checksum="16F53581AB59242C" modified="2009-07-28" version="1">MSRPPSAAMERRYSADARLDGDGDGCDALGGACAPPPAPPPAGASASVGAGLVAASAPPVVGYEPVPVRALYSTPAHSYQVLAGHVPSHMPGHMPGQPQGHIHSQSLHQFGAAASPASSASPGSVRRVGRRFWQPSEDIVLLDTVLAHRYEVPISPNVRHFWDSVSRQLFQEHALQRNTRQCRDRFNLLYARGVRNAACNVEPENARDALILKVARVFHATDQGHYRMSPGADTSTDTSMGPSAGILTAPAGATPQAGNSMDWNYMANSVSSLVASTRSLAAEVRDLSERFTQLSQQVRYIQVRLACAPERSATGQFGAPDLPYDSQFHNPPAYPPPGGRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DI87</accession>
    <name>C5DI87_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Reticulon-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0E10560g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Endoplasmic reticulum membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553935.1">
        <property type="nucleotide sequence ID" value="XM_002553889.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DI87"/>
    <dbReference type="FunCoup" id="C5DI87">
        <property type="interactions" value="287"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DI87"/>
    <dbReference type="GeneID" id="8292100"/>
    <dbReference type="KEGG" id="lth:KLTH0E10560g"/>
    <dbReference type="eggNOG" id="KOG1792">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050576_0_0_1"/>
    <dbReference type="InParanoid" id="C5DI87"/>
    <dbReference type="OMA" id="HYVNILR"/>
    <dbReference type="OrthoDB" id="567788at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009617">
        <property type="term" value="P:response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003388">
        <property type="entry name" value="Reticulon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045064">
        <property type="entry name" value="Reticulon-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10994">
        <property type="entry name" value="RETICULON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10994:SF193">
        <property type="entry name" value="RETICULON-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02453">
        <property type="entry name" value="Reticulon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50845">
        <property type="entry name" value="RETICULON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="127"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="Reticulon" evidence="8">
        <location>
            <begin position="3"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="252"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="254"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR23498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="310" mass="33029" checksum="D8B03BE1AB408A60" modified="2009-07-28" version="1">MSSNELLYWRNPVETGKVFGGILVSLLVMKKVNLITCLLKVMYTIMLTTASVEFLSKLFLGQGMVTKYGLQSCPDAVGFLKPRIEAALNAIPPRQAQLRKLVMAQSPKCTFKAAGILYVLHKLFSVVSLWTALFVGTILTFSLPLIYKSYQKEIDATVAQGVEAGKQHASALQSTVCEKASPYVKQLDEKLGPVSGFVKSKLPATRVGGSNITAESTTTKLAADVPFEKPAPAKTSASAFPAATSHIPTETPLASADAASAPATAKTSSADFPSVPSTDFSKIQATEQINETVKDATTGISHSTDPLKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-04-02" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIH9</accession>
    <name>C5DIH9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0E12650p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0E12650g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554027.1">
        <property type="nucleotide sequence ID" value="XM_002553981.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIH9"/>
    <dbReference type="GeneID" id="8292195"/>
    <dbReference type="KEGG" id="lth:KLTH0E12650g"/>
    <dbReference type="eggNOG" id="ENOG502SAYP">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1875803_0_0_1"/>
    <dbReference type="InParanoid" id="C5DIH9"/>
    <dbReference type="OMA" id="MAREGNT"/>
    <dbReference type="OrthoDB" id="4065597at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17242">
        <property type="entry name" value="DUF5315"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="136" mass="15751" checksum="580C2219BB9D721C" modified="2009-07-28" version="1">MHSSASSHLSSSSAHEKTKSAHTEPEDSSIPPIRTLPRQTHQIEAHPNVDYQDKLWTQIDVLDDVRRMAREQDTYEGFPPAFESQLQKLRGAHVTLLQAMRNRRDKQDADHENEDENEVEHKLVDDVMNCLQELRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DII4</accession>
    <name>C5DII4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Mediator of RNA polymerase II transcription subunit 8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">Mediator complex subunit 8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MED8</name>
        <name evidence="11" type="ordered locus">KLTH0E12760g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the Mediator complex subunit 8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554032.1">
        <property type="nucleotide sequence ID" value="XM_002553986.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DII4"/>
    <dbReference type="FunCoup" id="C5DII4">
        <property type="interactions" value="180"/>
    </dbReference>
    <dbReference type="GeneID" id="8292200"/>
    <dbReference type="KEGG" id="lth:KLTH0E12760g"/>
    <dbReference type="eggNOG" id="ENOG502S8U1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108151_0_0_1"/>
    <dbReference type="InParanoid" id="C5DII4"/>
    <dbReference type="OMA" id="PQWYSLQ"/>
    <dbReference type="OrthoDB" id="5329317at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019364">
        <property type="entry name" value="Mediatior_Med8_fun/met"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13074">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13074:SF9">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10232">
        <property type="entry name" value="Med8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0010">Activator</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="180"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="184"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR23595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="218" mass="24319" checksum="231A015E6F8BC666" modified="2009-07-28" version="1">MSQGPVPDTGVGVADSQADYDFSHVPAQALDAVRMRLAQLTHSQAKLKDEMARADLPQWHSLQAQVSIILTQLQSLTSTIHHFEETLDSTVVYPLANFPTTAHEGLLTTLLRKKNIPEVDEWINDAKETSGIDINTASHEDIKTALRSDEELTKWALDFLKQEHSNYSYHGFYTARELSAGASPDTQDTYQRAPSSKKPKKPFDANNVLKMIHQGTFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIK6</accession>
    <name>C5DIK6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Translocation protein SEC62</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0E13266g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SEC62 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554054.1">
        <property type="nucleotide sequence ID" value="XM_002554008.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIK6"/>
    <dbReference type="FunCoup" id="C5DIK6">
        <property type="interactions" value="155"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIK6"/>
    <dbReference type="GeneID" id="8292223"/>
    <dbReference type="KEGG" id="lth:KLTH0E13266g"/>
    <dbReference type="eggNOG" id="KOG2927">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040936_1_0_1"/>
    <dbReference type="InParanoid" id="C5DIK6"/>
    <dbReference type="OMA" id="FKPLYGW"/>
    <dbReference type="OrthoDB" id="200187at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031204">
        <property type="term" value="P:post-translational protein targeting to membrane, translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004728">
        <property type="entry name" value="Sec62"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011553">
        <property type="entry name" value="Sec62_asco"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00869">
        <property type="entry name" value="sec62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12443">
        <property type="entry name" value="TRANSLOCATION PROTEIN SEC62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12443:SF9">
        <property type="entry name" value="TRANSLOCATION PROTEIN SEC62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03839">
        <property type="entry name" value="Sec62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0811">Translocation</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="133"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="159"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR23617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="243" mass="28094" checksum="7CE191286992D9F9" modified="2009-07-28" version="1">MSNPASTVAIGSLLRRHKLLKQRQGLFQSRHVDFFRYKRFVRALNAREYQSKSANQPDLYPVVDNEEDARKVFIELIKAQLVVPCRKLHSAECKDHGLKPDKDYPNLLLSDKATLQPDEYYAWNFNPKTLTDYLLVLGIVAGILAFVCYPLWPSSMRRVVYYISLALLALIGLFFAVAILRFIIYLLSLAVCSEKGGFWLFPNLFEDCGVLESFKPLYGSGESECYSYIKKMKRRKRKMNKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIM5</accession>
    <name>C5DIM5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0E13684p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0E13684g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554073.1">
        <property type="nucleotide sequence ID" value="XM_002554027.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIM5"/>
    <dbReference type="FunCoup" id="C5DIM5">
        <property type="interactions" value="62"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIM5"/>
    <dbReference type="GeneID" id="8292242"/>
    <dbReference type="KEGG" id="lth:KLTH0E13684g"/>
    <dbReference type="eggNOG" id="KOG4690">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062297_1_0_1"/>
    <dbReference type="InParanoid" id="C5DIM5"/>
    <dbReference type="OMA" id="CVWEIYN"/>
    <dbReference type="OrthoDB" id="10064411at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019180">
        <property type="entry name" value="Oxidoreductase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039251">
        <property type="entry name" value="OXLD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21193">
        <property type="entry name" value="OXIDOREDUCTASE-LIKE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21193:SF3">
        <property type="entry name" value="OXIDOREDUCTASE-LIKE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09791">
        <property type="entry name" value="Oxidored-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxidoreductase-like" evidence="2">
        <location>
            <begin position="67"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR23636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="225" mass="25700" checksum="4281E94299AD8B6A" modified="2009-07-28" version="1">MIPTPRFCGFVRRSLKTTAKQLMTFEGSSLGVIGNDEEKMNNVFGGRIKGEPRRSTSRMIKRGSRDIAGIPVPERPIEPDNCCMSGCVNCVWEIYNEDLKEWKDRRKQAAAKLKGTDTKWPADFDPPLSFLDIRNVPSNLKTLKIKLDKQKQRGTSGLFPPREDKLPQSVLDAKRKRREERAESAQALGIQEDQNEGWDNIPVFIRVFAEFEKKKKEAKQSQKSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIY4</accession>
    <name>C5DIY4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0E16126g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554182.1">
        <property type="nucleotide sequence ID" value="XM_002554136.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIY4"/>
    <dbReference type="FunCoup" id="C5DIY4">
        <property type="interactions" value="418"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIY4"/>
    <dbReference type="GeneID" id="8292353"/>
    <dbReference type="KEGG" id="lth:KLTH0E16126g"/>
    <dbReference type="eggNOG" id="KOG0198">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006413_0_0_1"/>
    <dbReference type="InParanoid" id="C5DIY4"/>
    <dbReference type="OMA" id="KYHGFIQ"/>
    <dbReference type="OrthoDB" id="8693905at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050629">
        <property type="entry name" value="STE20/SPS1-PAK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012:SF26">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE DDB_G0283821-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="23"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR23745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="868" mass="98219" checksum="ADA20855DDF21788" modified="2009-07-28" version="1">MATRVNVTPAQKIPSASSNTHHYALKQVVGKGAYGIVYRAINRQTRKQVAIKVIEYDEEEELNEHMLEIDLLKNLKHENIVKYHGFIQKSHQLFILLEYCSQGSLRDLIKRGPVDEQECKTYIRQTLHGLKYLHEQGVIHRDIKAANLLLDAENVVKLADFGVSTRVNNLAMTYAGSPNWMAPEVMVGEGASTVSDIWSLGATVVEILTGNPPFHNLVNEAACYAIVHDAYIPPKTLSSTCRKFLRACFQKSMFKRPSAEELLNHEWLYETKQPILEKFKENAADDWGRDFIEVEVSPFKEHDVNNGQQNLIRLSQSCGQCNPHIIYSECSFTDIAWCLIDLCRRGQLNNVHEMFAYDLQYNSGRCRDLFIALGGISALLCHLGSTEKILNVFFIEHATDMIKCGILPHCKLLTSPQLILSTVSAYYEIVDFDSWCRWCSGLPNLGNAIAAELEKEDSLAEETLLRLSEAVSFPLEKEFVMKLMSLPLEKSSKLSYIIFKSFNNFLEKKEVASSLSDSYDSNILSTPHSTPMPSNSRIHNFPSFTGRTILPEGFTEWLLKLTPTASSDPHLLKNFIELCFNASHLNNTVLGEIVEHGGFFKLIRQLTEHFERDSKKKYTKTMLSIALNLCVELSQELTKPFLSCAVDVALRFLKIPEFITGGTEILLHCFLFAIHENFEVKEDSSDILIHCDQACIQIPRKALLRTFYSEEPKFGNYITKFTKLCSLPPCGTLATEIVSHPLFMEKTKTLFAVYRSSLIIQIDFLKFLKLILTRYAEYAPRSTKKDITSEALISSAILQRDSEPYHDVISSVADFLRSNWQTEGSLQGQVGTDSVLIRQLCYDISLLGAEKPLINPKRVDKSGFVVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJ38</accession>
    <name>C5DJ38_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">KLTH0F13222p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0F13222g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554764.1">
        <property type="nucleotide sequence ID" value="XM_002554718.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJ38"/>
    <dbReference type="FunCoup" id="C5DJ38">
        <property type="interactions" value="1172"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJ38"/>
    <dbReference type="GeneID" id="8292988"/>
    <dbReference type="KEGG" id="lth:KLTH0F13222g"/>
    <dbReference type="eggNOG" id="KOG2484">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011106_5_5_1"/>
    <dbReference type="InParanoid" id="C5DJ38"/>
    <dbReference type="OMA" id="FKLDGLW"/>
    <dbReference type="OrthoDB" id="10266128at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030684">
        <property type="term" value="C:preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd04178">
        <property type="entry name" value="Nucleostemin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1580.10:FF:000006">
        <property type="entry name" value="Nuclear GTP-binding protein NUG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000844">
        <property type="entry name" value="Nuclear GTP-binding protein NUG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030378">
        <property type="entry name" value="G_CP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014813">
        <property type="entry name" value="Gnl3_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023179">
        <property type="entry name" value="GTP-bd_ortho_bundle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050755">
        <property type="entry name" value="TRAFAC_YlqF/YawG_RiboMat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11089">
        <property type="entry name" value="GTP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11089:SF30">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN-LIKE 3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08701">
        <property type="entry name" value="GN3L_Grn1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51721">
        <property type="entry name" value="G_CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="CP-type G" evidence="10">
        <location>
            <begin position="165"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="459"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="62"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="21"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="459"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR24327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="520" mass="57120" checksum="CA0E30F729F49D6D" modified="2009-07-28" version="1">MKVRKRSSKRTSTRMREGIKKKAAAQHRKERKLAKKDPTWKSNKKRDPGIPASFPYKNKILEAIEVRKAQEAEEKLANLQRKLEEKQRAAESGEIIMDEMEDDEGGESDLTALVESAQQAAAEFNGENGVDQESSEPQEVVEYDIDFFAGDSESTTELEKSRKAYDKIFKTVVDASDVILYVLDARNPEGTRSRKVEQAVLQSQGKRLILILNKVDLIPPFVLEQWLAFLKSSFPTIPLRAAPGATNASSFNKKLSQSGTAAALLESLKTYANNSNFKRSIVVGVIGYPNVGKSSVINALTSRRGGFSKVCPVGNQAGVTTSLREVKIDNKLKILDSPGICFPSDTGKKTKKEQEAELALLNALPAKHIVDPYPAVLMLVKRLSKSEEMTEAFKRLYDLPPIPAADANSFTKQFLIHVARKRGRIGKGGIPNLSSAGISVLNDWRDGKILGWVLPNSSKDGSHNEASTGGSAIESKKAPQRVEQTSVVSEWSKEFDLDGLFASLDNAVANENQNGDADMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJ48</accession>
    <name>C5DJ48_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Actin cytoskeleton-regulatory complex protein PAN1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Actin cytoskeleton-regulatory complex protein pan1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ordered locus">KLTH0F13464g</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Actin patch</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PAN1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554774.1">
        <property type="nucleotide sequence ID" value="XM_002554728.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DJ48">
        <property type="interactions" value="80"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJ48"/>
    <dbReference type="GeneID" id="8292998"/>
    <dbReference type="KEGG" id="lth:KLTH0F13464g"/>
    <dbReference type="eggNOG" id="KOG0998">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006042_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJ48"/>
    <dbReference type="OMA" id="GMPGQWG"/>
    <dbReference type="OrthoDB" id="2015333at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
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</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJ51</accession>
    <name>C5DJ51_LACTC</name>
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            <fullName evidence="2">KLTH0F13552p</fullName>
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        <name evidence="2" type="ordered locus">KLTH0F13552g</name>
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        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
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        </source>
    </evidence>
    <sequence length="751" mass="79879" checksum="9D4AF728674CEB46" modified="2009-07-28" version="1">MALQDIKEEPEQTSAERQSVRDHSEMDDDSLREQLEAKQAVEPQPEAVLQPAVSGLGLSGEAVQAAFQTQHSPDSSSEALLPPLPDVYSVNASPTPPMSPGTPKSITDSPVVSVPRSGSDLNAHTQSSASPGSQIRRARAASLLDAAETAASGSLGSTGTADSVLSRTIDHESDINTSFDLSKALEIPQDSTVSGAGSNPNSNPISPTISSPKISFKKKFSSTLLPAATSGIAGSHTNGDTSANAAAVKAVVGSPEFSLHHLQRHGSVRSKGNTPELPRLSSSVEHHSGLGIKDGNLDNSEKRMLRGYSVDSSSSSSQSFYDNDDLAFRPPLSLLEADVGSLASLHDLKSPRSSLNYSEAADNANAKKIPLLKRASSAILRKTSLSNKNSSSQSPNLSSVSGLNPSNYHELRQTASFSTIPYDSPSSEMSALSSRNRKKLSLKTSASQNRCISNPEGLRNVASLPTPTAGTGVQTPVTNQSLGSKFRCGLTRIISGGGTERSRSNSGFNGSGAVHRSISGIERTPSSMLAESPLEESFGNPLGEGPFERLARGGQGGLKKSASFSHRSHISPSNNATVGSDLVAKKSQLSKKSTTHSAGSVTTVRNDSVFSGTSSDSSSNSSETDIITVDIDELTKALPVITVTEKFGARHVTPIQTQSNILLDRIYKDESDKALAGHAAEKPNRITLREYINVLMKQQQIEDERFAVLEKNFSNNGWCSQEDLNNIQQKRFIINKKWAERISYYQDRLEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJ79</accession>
    <name>C5DJ79_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0F14190p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0F14190g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the universal ribosomal protein uS12 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554805.1">
        <property type="nucleotide sequence ID" value="XM_002554759.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJ79"/>
    <dbReference type="FunCoup" id="C5DJ79">
        <property type="interactions" value="898"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJ79"/>
    <dbReference type="GeneID" id="8293031"/>
    <dbReference type="KEGG" id="lth:KLTH0F14190g"/>
    <dbReference type="eggNOG" id="KOG1749">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115574_0_1_1"/>
    <dbReference type="InParanoid" id="C5DJ79"/>
    <dbReference type="OMA" id="KFRWSQR"/>
    <dbReference type="OrthoDB" id="1713912at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03367">
        <property type="entry name" value="Ribosomal_S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000007">
        <property type="entry name" value="40S ribosomal protein S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006032">
        <property type="entry name" value="Ribosomal_uS12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005680">
        <property type="entry name" value="Ribosomal_uS12_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00982">
        <property type="entry name" value="uS12_E_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11652">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S12 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00164">
        <property type="entry name" value="Ribosom_S12_S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002133">
        <property type="entry name" value="Ribosomal_S12/S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00055">
        <property type="entry name" value="RIBOSOMAL_S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR24368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="147" mass="16287" checksum="7C76623E4E6991B4" modified="2009-07-28" version="1">MGKGKPRGLNAARKLRVHRRNNYSRWAEQSYKKRLLGTAFKSSPFGGSSHAKGIVLEKIGIESKQPNSAIRKCVRVQLIKNGKRVTAFVPNDGCLNFVDENDEVLLAGFGRKGKAKGDIPGVRFKVVKVSGVSLLALWKEKKEKPRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJ84</accession>
    <name>C5DJ84_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Conserved oligomeric Golgi complex subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Component of oligomeric Golgi complex 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0F14300g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554810.1">
        <property type="nucleotide sequence ID" value="XM_002554764.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJ84"/>
    <dbReference type="FunCoup" id="C5DJ84">
        <property type="interactions" value="79"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJ84"/>
    <dbReference type="GeneID" id="8293036"/>
    <dbReference type="KEGG" id="lth:KLTH0F14300g"/>
    <dbReference type="eggNOG" id="ENOG502RYA4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095072_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJ84"/>
    <dbReference type="OMA" id="YVHHLRN"/>
    <dbReference type="OrthoDB" id="4034328at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054494">
        <property type="entry name" value="COG2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024602">
        <property type="entry name" value="COG_su2_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06148">
        <property type="entry name" value="COG2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22431">
        <property type="entry name" value="COG2p_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Conserved oligomeric Golgi complex subunit 2 N-terminal" evidence="8">
        <location>
            <begin position="27"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="Conserved oligomeric Golgi complex subunit 2 C-terminal" evidence="9">
        <location>
            <begin position="98"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF06148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF22431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR24373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="248" mass="28481" checksum="4D9AD7FB94E7DCC8" modified="2009-07-28" version="1">MNLLDESWDLPVSKNVSRELFTAYAENDTFDEDSFLLENNFQYVPLNTLAKDLQELTQQMDKVMLETSSSSYNDYTKLCEQFSDSAEARPELQGVRMDIAKFLDQLRELKDTNISNTREVVTDTVDYLRTLDRLSETLQECKVLSSMMELGRKLCKRLDSLCQETDLEVSLCSDLVLQIHQISSKSHTLLLKMQEIESPLLTQIRIEHQSIADQFRACLHHLCDKCLERPTETEELGATLAQLLAQSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJ87</accession>
    <name>C5DJ87_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ammonium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0F14410g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ammonia transporter channel (TC 1.A.11.2) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554813.1">
        <property type="nucleotide sequence ID" value="XM_002554767.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJ87"/>
    <dbReference type="FunCoup" id="C5DJ87">
        <property type="interactions" value="389"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJ87"/>
    <dbReference type="GeneID" id="8293041"/>
    <dbReference type="KEGG" id="lth:KLTH0F14410g"/>
    <dbReference type="eggNOG" id="KOG0682">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_33_0_1"/>
    <dbReference type="InParanoid" id="C5DJ87"/>
    <dbReference type="OMA" id="FNAGSWL"/>
    <dbReference type="OrthoDB" id="534912at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008519">
        <property type="term" value="F:ammonium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019740">
        <property type="term" value="P:nitrogen utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3430.10:FF:000003">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3430.10">
        <property type="entry name" value="Ammonium transporter AmtB like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029020">
        <property type="entry name" value="Ammonium/urea_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001905">
        <property type="entry name" value="Ammonium_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018047">
        <property type="entry name" value="Ammonium_transpt_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00836">
        <property type="entry name" value="amt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43029:SF4">
        <property type="entry name" value="AMMONIUM TRANSPORTER MEP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43029">
        <property type="entry name" value="AMMONIUM TRANSPORTER MEP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111352">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01219">
        <property type="entry name" value="AMMONIUM_TRANSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0924">Ammonia transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="137"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="175"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="203"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="235"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="263"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="287"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="319"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="369"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="Ammonium transporter AmtB-like" evidence="9">
        <location>
            <begin position="15"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR24376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="453" mass="49835" checksum="A4A3F81EFDC66BFF" modified="2009-07-28" version="1">MGQLWEETYEPATVAFILVGAALVFVMVPGLGFLYSGLTRRKSALAQIWAVLMAALVGMVQWYFWGYSLAFSKTSHNHKFIGNLDSFGFRNVYGKASEDSEYPEIAFAAFQGLFMCVTLSIIAGCTAERGRLLPHMVFLFCFATLVYCPVTYWIWASDGWAYRWGVLDWAGGGPVEILSSAGGFVYSAFLGKRKESLLINFRPHNVSMVTLGTSLLWFGWLGFNSCTSLTPNLKSAYAFMNTNLSAAVGGMTWCLLDFRLEKKWSTVGMCSGIICGLVAATPSSGCITLYGSVIQGIVAGIVCNFSTKIKYYLKVDDSMDILAEHGIAGVVGLFFNALFSADWVVGMDGATEHGGGWITHNYKQMYKQIAYIGAVFGYGVAVTAIICFVIDKIPGLQLRASEEAEERGLDEDQIGEFAYDYVEVRRDYFSWGVNNKAESLSEDNDSHPQGEKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJE1</accession>
    <name>C5DJE1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0F15686p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0F15686g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554867.1">
        <property type="nucleotide sequence ID" value="XM_002554821.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJE1"/>
    <dbReference type="FunCoup" id="C5DJE1">
        <property type="interactions" value="241"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJE1"/>
    <dbReference type="GeneID" id="8293098"/>
    <dbReference type="KEGG" id="lth:KLTH0F15686g"/>
    <dbReference type="eggNOG" id="KOG3035">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051989_0_3_1"/>
    <dbReference type="InParanoid" id="C5DJE1"/>
    <dbReference type="OMA" id="VPIDRYK"/>
    <dbReference type="OrthoDB" id="671439at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="CDD" id="cd01838">
        <property type="entry name" value="Isoamyl_acetate_hydrolase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1110:FF:000022">
        <property type="entry name" value="Isoamyl acetate-hydrolyzing esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045136">
        <property type="entry name" value="Iah1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013830">
        <property type="entry name" value="SGNH_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14209">
        <property type="entry name" value="ISOAMYL ACETATE-HYDROLYZING ESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14209:SF19">
        <property type="entry name" value="ISOAMYL ACETATE-HYDROLYZING ESTERASE 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13472">
        <property type="entry name" value="Lipase_GDSL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SGNH hydrolase-type esterase" evidence="1">
        <location>
            <begin position="10"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="240" mass="27274" checksum="F274CCD07F3D7E18" modified="2009-07-28" version="1">MSLKYQKFLLFGDSITEFAFNTRMSEDKGDQFSLGAALANVYTRKLDVVQRGFSGYNTRWALKLLPRILESEPTDDIVLSTVFFGSNDAVHDGSQKVELPEFIQINIDLVRLLKSKGIKPILIGPALHDADKWKSLRPEEVAKGVVRSNENNKRYSDALQEVARTENVAFVNLIETFSNQGGSDWRSLLNDGLHFSGKGYEVFYNELLKTIRVAYPEWAPENVTYKYPNWRDVEPDGSNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJN2</accession>
    <name>C5DJN2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0F17798p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0F17798g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleoporin Nup133 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554958.1">
        <property type="nucleotide sequence ID" value="XM_002554912.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJN2"/>
    <dbReference type="FunCoup" id="C5DJN2">
        <property type="interactions" value="174"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJN2"/>
    <dbReference type="GeneID" id="8293193"/>
    <dbReference type="KEGG" id="lth:KLTH0F17798g"/>
    <dbReference type="eggNOG" id="KOG4121">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_274661_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJN2"/>
    <dbReference type="OMA" id="HVATLLW"/>
    <dbReference type="OrthoDB" id="103454at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031080">
        <property type="term" value="C:nuclear pore outer ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000972">
        <property type="term" value="P:transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014908">
        <property type="entry name" value="Nucleoporin_Nup133/Nup155_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037624">
        <property type="entry name" value="Nup133-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13405">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13405:SF11">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08801">
        <property type="entry name" value="Nucleoporin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117289">
        <property type="entry name" value="Nucleoporin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Nucleoporin Nup133/Nup155-like N-terminal" evidence="5">
        <location>
            <begin position="54"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR24521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1131" mass="128354" checksum="5A238BA314CCE056" modified="2009-07-28" version="1">MSSKPLFQLRKELDSSNEAASDTEVTQSFVEQFQVSNNIEPYVSDKFTNEVVLTENEKYTVSKLPSSLNFLPESPDLDGLVDAHSGNAIVNDNDNLYIWAYRSTQAQPSVSRIPLHEEHISQSSVPKVVFTWPAAMDDESAAQSSGVCIVNRKSGLVQFYEDVDTINHLSSLLSKSKCHELNLKLKDKETVEDVVNCEPAGILISTSKGRALFVTIRDFSGKPRLHLKAQLVKSHLGFFFSPNKFKKVVSIKPGHIPGKGERLAFILTSGGDFHVWNLSAVSNCHRKISINLYDQILESLQDLYPFAHSSLLLLDSHPLDTEGSAHMVLSSITNYDGACYYILTTIKIDESTNSFAIFSTYRLNTYVSAFSEENRPRLIVPLSSNQDASESTEPSITSVYTIFPDAVVLTQVSAKLDQTYSLRRKWEDIISFRDDVNIIGHGNDSESVYLISRGMGVLMVTTSKTGNTSGFHDVRFIKSHIQQAVYFSGLSSSPIDFNLSPDISLQREEIEEDLLASSNEILLSKSIYIPPKLKSLERHASMRVGLYRRLLAFTKSNFLNKISPSVKIELIESFEVLNASSQLLPYTEKSKETKEVWLRVLEANGVTEEDFMRHDLDKFPKVLSQFLQQLQLTLASTTHNKLWPQCIEMIISCLYRGALEDGEEYYRFGMFEMDKHELHSQLPWYVQHSIPEAVNNLLYGLVDYSKMSQDSEQHADQILSLVKALYYMSNQTSLWLQQDTKRADNNDCTSVSKLFKENRLSWNEMLCDIGRSSDSLHICEFYEDLPSLAQTLNTLPNEASEGLYTHYFQKFGYNFAAQLFTNYITHGRLQELYNKFGCQNSYLKKFFDENLQFAEVGWMEKILNEQYSEAADILTKISTDNKKAGNLDRDQTRLSVAKLCVLASEASYEFENLTMIQSGLDIIDGQKDLAAMLESGIKLHKRFEECSAVQKLYKRLSENISASNRLTLSEVVEMYTILNSKEGFFRALKLLSLVKVSMDFEKIKFLEIMIWRRCILFDNMRSLEETSSALFHTLKKSFDDQLYLSAHFTLPSLTALLDKTTVTSEYFAAEYDSRKDDVRGLFESVAEEQSEIMELSDELSPQIKSVIAASNDSSTQKCVINYETNQIEGLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJW7</accession>
    <name>C5DJW7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 9">6,7-dimethyl-8-ribityllumazine synthase</fullName>
            <shortName evidence="9">DMRL synthase</shortName>
            <ecNumber evidence="4 9">2.5.1.78</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0F19690g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 9">
            <text>(2S)-2-hydroxy-3-oxobutyl phosphate + 5-amino-6-(D-ribitylamino)uracil = 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:26152"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58201"/>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
            <dbReference type="EC" id="2.5.1.78"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homopentamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the DMRL synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.78" evidence="4 9"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555043.1">
        <property type="nucleotide sequence ID" value="XM_002554997.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJW7"/>
    <dbReference type="FunCoup" id="C5DJW7">
        <property type="interactions" value="345"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJW7"/>
    <dbReference type="GeneID" id="8293279"/>
    <dbReference type="KEGG" id="lth:KLTH0F19690g"/>
    <dbReference type="eggNOG" id="KOG3243">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089358_2_2_1"/>
    <dbReference type="InParanoid" id="C5DJW7"/>
    <dbReference type="OMA" id="CQGVTQG"/>
    <dbReference type="OrthoDB" id="2965at2759"/>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00404"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009349">
        <property type="term" value="C:riboflavin synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000906">
        <property type="term" value="F:6,7-dimethyl-8-ribityllumazine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd09209">
        <property type="entry name" value="Lumazine_synthase-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.960:FF:000007">
        <property type="entry name" value="6,7-dimethyl-8-ribityllumazine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.960">
        <property type="entry name" value="Lumazine/riboflavin synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00178">
        <property type="entry name" value="Lumazine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034964">
        <property type="entry name" value="LS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002180">
        <property type="entry name" value="LS/RS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036467">
        <property type="entry name" value="LS/RS_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00114">
        <property type="entry name" value="lumazine-synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21058:SF0">
        <property type="entry name" value="6,7-DIMETHYL-8-RIBITYLLUMAZINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21058">
        <property type="entry name" value="6,7-DIMETHYL-8-RIBITYLLUMAZINE SYNTHASE DMRL SYNTHASE LUMAZINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00885">
        <property type="entry name" value="DMRL_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52121">
        <property type="entry name" value="Lumazine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0686">Riboflavin biosynthesis</keyword>
    <keyword evidence="6 9" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00072606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR24606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="166" mass="18040" checksum="FA9B31AAB1B95660" modified="2009-07-28" version="1">MAVKGLGQIDQQYDGSKLRIGIIHARWNRVIIDALVKGAIARMSELGVKEENIIVETVPGSFELPFGSKKFVEQQSSKGTPLDVVIPIGVLIKGSTMHFEYISESTTNALMALQDKVDIPVIFGLLTCLTEEQAMARAGIDEAKTMHNHGEDWGAAAVEMAVKFGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJW8</accession>
    <name>C5DJW8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0F19712p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0F19712g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555044.1">
        <property type="nucleotide sequence ID" value="XM_002554998.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJW8"/>
    <dbReference type="FunCoup" id="C5DJW8">
        <property type="interactions" value="195"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJW8"/>
    <dbReference type="GeneID" id="8293280"/>
    <dbReference type="KEGG" id="lth:KLTH0F19712g"/>
    <dbReference type="eggNOG" id="ENOG502QQ4K">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020873_1_0_1"/>
    <dbReference type="InParanoid" id="C5DJW8"/>
    <dbReference type="OMA" id="TKLWGYE"/>
    <dbReference type="OrthoDB" id="21643at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="5"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="207"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="277"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR24607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="460" mass="53636" checksum="3CAD29168F13BAD1" modified="2009-07-28" version="1">MTTEKRLFVGNIFNDPEGSLKELYHRFQRFGKCISTEFERYDHFGYIDMEFDNEAAFLKLKQSFNGVKFKGNALKVDIAKMGWKERWEMEQEQGQRVEQVKEKQIRKSQWEHFKKLENINMSWTDRQQLIAGRLRRTPRGRAQLRNITFRVNQDGELKVYKCRKNKLWGYERKKGPRDLVCKFTDHHYWRDGNDHIVDRLDYSRAAPHWGPRRDTNIQTTEDDHSEEESFEKDKTKDVLANILGNFDFDKPLALEEDEGEDAASDYEYNALFEGNEASGRKTEVAAQPAAEKSPEASEDEGQEEFIPTFAPAGTEAESSTGGENNVEKLRSLFNPGHQGADFRLIDESDEDIDNEKSVPQTDVPYEEEELVLETHVDAEAPTHSSKGLFFPHFTSPFLHSQSQLAKLKPPTDHQELFSNWENHYWENRAAWTKEMKSKKRDAIRQLKRKNFKAKGNGILI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJY4</accession>
    <name>C5DJY4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 15">Dihydroorotate dehydrogenase (fumarate)</fullName>
            <ecNumber evidence="7 15">1.3.98.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14 15">Dihydroorotate oxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">KLTH0F00308g</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes the conversion of dihydroorotate to orotate with fumarate as the electron acceptor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>(S)-dihydroorotate + fumarate = orotate + succinate</text>
            <dbReference type="Rhea" id="RHEA:30059"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
            <dbReference type="ChEBI" id="CHEBI:30864"/>
            <dbReference type="EC" id="1.3.98.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 15">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 15">Pyrimidine metabolism; UMP biosynthesis via de novo pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 15">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 15">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 15">Belongs to the dihydroorotate dehydrogenase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.98.1" evidence="7 15"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554222.1">
        <property type="nucleotide sequence ID" value="XM_002554176.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJY4"/>
    <dbReference type="FunCoup" id="C5DJY4">
        <property type="interactions" value="753"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJY4"/>
    <dbReference type="GeneID" id="8292412"/>
    <dbReference type="KEGG" id="lth:KLTH0F00308g"/>
    <dbReference type="eggNOG" id="KOG1436">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042042_3_0_1"/>
    <dbReference type="InParanoid" id="C5DJY4"/>
    <dbReference type="OMA" id="FDFAHFD"/>
    <dbReference type="OrthoDB" id="14784at2759"/>
    <dbReference type="UniPathway" id="UPA00070"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990663">
        <property type="term" value="F:dihydroorotate dehydrogenase (fumarate) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04741">
        <property type="entry name" value="DHOD_1A_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000027">
        <property type="entry name" value="Dihydropyrimidine dehydrogenase [NADP(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.26.10">
        <property type="entry name" value="Dihydroorotate Dehydrogenase A, chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00224">
        <property type="entry name" value="DHO_dh_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050074">
        <property type="entry name" value="DHO_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033886">
        <property type="entry name" value="DHOD_1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023359">
        <property type="entry name" value="Dihydro_DH_chainA_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024920">
        <property type="entry name" value="Dihydroorotate_DH_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012135">
        <property type="entry name" value="Dihydroorotate_DH_1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005720">
        <property type="entry name" value="Dihydroorotate_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001295">
        <property type="entry name" value="Dihydroorotate_DH_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002702">
        <property type="entry name" value="PRK02506.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48109:SF1">
        <property type="entry name" value="DIHYDROOROTATE DEHYDROGENASE (FUMARATE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48109">
        <property type="entry name" value="DIHYDROOROTATE DEHYDROGENASE (QUINONE), MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01180">
        <property type="entry name" value="DHO_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000164">
        <property type="entry name" value="DHO_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00911">
        <property type="entry name" value="DHODEHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00912">
        <property type="entry name" value="DHODEHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 15" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="11 15" id="KW-0288">FMN</keyword>
    <keyword evidence="13 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12 15" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dihydroorotate dehydrogenase catalytic" evidence="16">
        <location>
            <begin position="5"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00011911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00021374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00031623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU364042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAR23785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="314" mass="35050" checksum="CCF165F8103C2057" modified="2009-07-28" version="1">MPASLKTEFLGQSFENPFMNASGVHCMSKRELDELKNSGAGSYITKSSTTLSREGNPAPRYHSVPLGSINSMGLPNEGFDYYLDYVLQYQKENPENVPPFFSVAGMSLNENLELLKKIQDSEYRGITELNLSCPNVPGKPQVAYDFEITKEILTKAFEFFKKPLGVKLPPYFDFAHFDIMAKILNEYPLAYVNCINSIGNGLYIDIEREAVVIKPKDGFGGLGGEYVKPTALANVRAFYTRLNPSIRIIGTGGIRTGQDAFEHILCGATMLQVGTELYKEGASIFERLGKELKEIMEKKGYNSIEEFRGKLNNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DK44</accession>
    <name>C5DK44_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0F01650p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0F01650g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554282.1">
        <property type="nucleotide sequence ID" value="XM_002554236.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DK44"/>
    <dbReference type="FunCoup" id="C5DK44">
        <property type="interactions" value="1230"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DK44"/>
    <dbReference type="GeneID" id="8292473"/>
    <dbReference type="KEGG" id="lth:KLTH0F01650g"/>
    <dbReference type="eggNOG" id="KOG1769">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148322_4_2_1"/>
    <dbReference type="InParanoid" id="C5DK44"/>
    <dbReference type="OMA" id="MKIYCAR"/>
    <dbReference type="OrthoDB" id="442921at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="CDD" id="cd16116">
        <property type="entry name" value="Ubl_Smt3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000155">
        <property type="entry name" value="Ubiquitin-like protein SMT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022617">
        <property type="entry name" value="Rad60/SUMO-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10562">
        <property type="entry name" value="SMALL UBIQUITIN-RELATED MODIFIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11976">
        <property type="entry name" value="Rad60-SLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="1">
        <location>
            <begin position="16"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="94" mass="10832" checksum="CF146EB6B175513C" modified="2009-07-28" version="1">MSEPQQESKPDIKPETHINLKVSDGSSEIFFKIKRTTPLRRLMEAFAKRQGKEMDSLRFLYDGVRIQADQTPEDLDMEDNDIIEAHREQIGGAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DL44</accession>
    <name>C5DL44_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0F09834p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0F09834g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554632.1">
        <property type="nucleotide sequence ID" value="XM_002554586.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DL44"/>
    <dbReference type="STRING" id="559295.C5DL44"/>
    <dbReference type="GeneID" id="8292839"/>
    <dbReference type="KEGG" id="lth:KLTH0F09834g"/>
    <dbReference type="eggNOG" id="ENOG502S549">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_502540_0_0_1"/>
    <dbReference type="InParanoid" id="C5DL44"/>
    <dbReference type="OMA" id="EWWQSLW"/>
    <dbReference type="OrthoDB" id="4036671at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="206"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="381"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="497"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="236"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="381"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="402"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="505"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="542" mass="59494" checksum="452D6BB783DA876D" modified="2009-07-28" version="1">MVSNGAGEQDDPSNMSESNQEPAHTGWSSMANGAAGAHTEENGNGEKRPTEEEPTFNQEILVSDAMAKNSRQLLYAHIYNYLIQHKYYETSRRLLDEADVPLSKSFPEERQKSGELLHAKMLMNSRDTFLCEWWQSLWTLHEYVESQPVEKISSSRLLREPVTPILPQRPPSAQGFVPANSPWMQNQHFMQGGLGYGRMTSNPGMPTNMGPPRGNDVKAGKAGVPPASPLRKTAASYPQAQTTPGVSHTPTALSGSGAGVPSQIGVAANQMPAAQSALTTPLYTNLSSAARSTASSTTPQPQAPAKTMMKTFAREYQQMGPLPQGVANTPVAPDNMMLYMQQQREMMTRYQGDDDMEKAGMNGAWSANQQRLHEQYQKNMYMMQQQQQSKRAQQSRPYPRQQELSKQAMLAQNAQTTQSSQSGAHQLRHGIQEHKSEPTSNQQDSAIHQHASPRQPPASAYPTAGDMVSESSAGAVPTMDQQYLNMMMMQYSPTGLSPGLAQPAVNMNETSNTGQSSGPTDKFRDNLASEFFPLQPSSSQHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DL52</accession>
    <name>C5DL52_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0F10032p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0F10032g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Preautophagosomal structure membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATG16 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554640.1">
        <property type="nucleotide sequence ID" value="XM_002554594.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DL52"/>
    <dbReference type="FunCoup" id="C5DL52">
        <property type="interactions" value="53"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DL52"/>
    <dbReference type="GeneID" id="8292848"/>
    <dbReference type="KEGG" id="lth:KLTH0F10032g"/>
    <dbReference type="eggNOG" id="ENOG502S6TV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_158825_0_0_1"/>
    <dbReference type="InParanoid" id="C5DL52"/>
    <dbReference type="OMA" id="QLRNKDY"/>
    <dbReference type="OrthoDB" id="8949486at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22887">
        <property type="entry name" value="Atg16_CCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013923">
        <property type="entry name" value="Autophagy-rel_prot_16_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08614">
        <property type="entry name" value="ATG16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0072">Autophagy</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Autophagy-related protein 16" evidence="5">
        <location>
            <begin position="34"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="35"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR24203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="121" mass="14090" checksum="8F86A5C3418D5664" modified="2009-07-28" version="1">MSGLLLERLQERDSIEKNFSELFSEPFVDQKAPSRKASEDQLAALKSELESKDREISDLKATLKLKSKDTEHLNDEIITLNIENNLLEERLNKTKAEYDEIVQRWLKKVQQEANVMNDTLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DL79</accession>
    <name>C5DL79_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NEDD8-activating enzyme E1 catalytic subunit</fullName>
            <ecNumber evidence="4">6.2.1.64</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0F10714g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalytic subunit of the dimeric E1 enzyme, which activates NEDD8.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>ATP + [NEDD8 protein] + [E1 NEDD8-activating enzyme]-L-cysteine = AMP + diphosphate + [E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine.</text>
            <dbReference type="EC" id="6.2.1.64"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein neddylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ubiquitin-activating E1 family. UBA3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.64" evidence="4"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554667.1">
        <property type="nucleotide sequence ID" value="XM_002554621.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DL79"/>
    <dbReference type="FunCoup" id="C5DL79">
        <property type="interactions" value="1270"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DL79"/>
    <dbReference type="GeneID" id="8292879"/>
    <dbReference type="KEGG" id="lth:KLTH0F10714g"/>
    <dbReference type="eggNOG" id="KOG2015">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013325_13_0_1"/>
    <dbReference type="InParanoid" id="C5DL79"/>
    <dbReference type="OMA" id="PYLENYM"/>
    <dbReference type="OrthoDB" id="10255449at2759"/>
    <dbReference type="UniPathway" id="UPA00885"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019781">
        <property type="term" value="F:NEDD8 activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045116">
        <property type="term" value="P:protein neddylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.80:FF:000002">
        <property type="entry name" value="SUMO-activating enzyme subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.520">
        <property type="entry name" value="Ubiquitin activating enzymes (Uba3). Chain: B, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023318">
        <property type="entry name" value="Ub_act_enz_dom_a_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF6">
        <property type="entry name" value="NEDD8-ACTIVATING ENZYME E1 CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="1 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="THIF-type NAD/FAD binding fold" evidence="5">
        <location>
            <begin position="3"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU368009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR24230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="313" mass="35026" checksum="B2E345B3B60D0A73" modified="2009-07-28" version="1">MADLADVSILVLGAGGLGCEILKNLAMQGIPDIHVVDMDTIELTNLNRQFLFRESDIGQSKALVAARFINEKNIMGLGGRPVVVTAHFQDLTLLDRKFIERFTLIVSGLDSIEARRFMNMQLVRITFESRFEKCIPFIDGGSEGLKGHCKTIIPGFSACYECSLDTLPAKIESYPLCTVSNNPRLPEHVIEFLMSVQWAQQHPDRDFDFDSKEDLQWLMDEAHARARQFNIDTSKLTPQFVLGVIKNIVPSVASTNAIIAAQCCTEVSKLLYNEYDIGEAPNFLVYNGDDGCFAYSFVHERVSTCLVCRESIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLF5</accession>
    <name>C5DLF5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Probable 26S proteasome regulatory subunit p27</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0F12738g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554743.1">
        <property type="nucleotide sequence ID" value="XM_002554697.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLF5"/>
    <dbReference type="FunCoup" id="C5DLF5">
        <property type="interactions" value="1135"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLF5"/>
    <dbReference type="GeneID" id="8292966"/>
    <dbReference type="KEGG" id="lth:KLTH0F12738g"/>
    <dbReference type="eggNOG" id="KOG3129">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073146_1_1_1"/>
    <dbReference type="InParanoid" id="C5DLF5"/>
    <dbReference type="OMA" id="DWGGRGM"/>
    <dbReference type="OrthoDB" id="72325at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070682">
        <property type="term" value="P:proteasome regulatory particle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000107">
        <property type="entry name" value="26S proteasome non-ATPase regulatory subunit 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040815">
        <property type="entry name" value="Nas2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035269">
        <property type="entry name" value="PSMD9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12651">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12651:SF1">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18265">
        <property type="entry name" value="Nas2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nas2 N-terminal" evidence="3">
        <location>
            <begin position="37"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00068021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF18265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR24306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="240" mass="26555" checksum="3041282C4ECC8DEA" modified="2009-07-28" version="1">MEQRNVFDVLSGGSENPNADAELRIQLSRLSELSLPEVFDLKSKLETQLSQLFDDLQHQGVNLESSLVTEDGFPRSDIDVLQVRLIRRGINILRNDLKAVIVRTQELMADQFQRLAAKTQSIGGSEVTEYRIPFAAVTEVVIDSPSHSAVSIQSYIVNSHRRRLLTFLQGLTKGDKIVKLGTIHAGNNKSLGAIGPLVQTNEEKPVTIRVLRNGTFLNLTLVPSKKWAGPGLLGCRLVHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLG8</accession>
    <name>C5DLG8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0G00616p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0G00616g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555066.1">
        <property type="nucleotide sequence ID" value="XM_002555020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLG8"/>
    <dbReference type="FunCoup" id="C5DLG8">
        <property type="interactions" value="18"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLG8"/>
    <dbReference type="GeneID" id="8293324"/>
    <dbReference type="KEGG" id="lth:KLTH0G00616g"/>
    <dbReference type="eggNOG" id="KOG4503">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107711_0_0_1"/>
    <dbReference type="InParanoid" id="C5DLG8"/>
    <dbReference type="OMA" id="FGSYRVY"/>
    <dbReference type="OrthoDB" id="5961at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006998">
        <property type="term" value="P:nuclear envelope organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040202">
        <property type="entry name" value="Brl1/Brr6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018767">
        <property type="entry name" value="Brl1/Brr6_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28136">
        <property type="entry name" value="NUCLEUS EXPORT PROTEIN BRR6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28136:SF5">
        <property type="entry name" value="NUCLEUS EXPORT PROTEIN BRR6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10104">
        <property type="entry name" value="Brr6_like_C_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01042">
        <property type="entry name" value="Brr6_like_C_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="34"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="148"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="Brl1/Brr6" evidence="3">
        <location>
            <begin position="34"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR24629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="193" mass="21777" checksum="FD5DB8C6F7A40DED" modified="2009-07-28" version="1">MYDQLVTSQKKRSVSSAASSSKESKRHWSDPKTLAAYIQLFVNTVIISGLLYLCVRFLRLVNRDVECKFRNKVALAAKAALECRANYETNRCEPEERAPALEAQCAEWAHCMSRSQELWDLQHSAVLWAETLAEIINGFFKPISIKSLIILLTFTFGVIIVSNIAFGSYRVHYYGALNSPQNSDLSSRGPRPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLN1</accession>
    <name>C5DLN1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0G02024p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0G02024g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555129.1">
        <property type="nucleotide sequence ID" value="XM_002555083.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLN1"/>
    <dbReference type="FunCoup" id="C5DLN1">
        <property type="interactions" value="64"/>
    </dbReference>
    <dbReference type="GeneID" id="8293388"/>
    <dbReference type="KEGG" id="lth:KLTH0G02024g"/>
    <dbReference type="eggNOG" id="ENOG502QPK1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_46_1_1"/>
    <dbReference type="InParanoid" id="C5DLN1"/>
    <dbReference type="OMA" id="YHAVMTW"/>
    <dbReference type="OrthoDB" id="5296287at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015355">
        <property type="term" value="F:secondary active monocarboxylate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035879">
        <property type="term" value="P:plasma membrane lactate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17316">
        <property type="entry name" value="MFS_SV2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508:SF10">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="120"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="146"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="170"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="204"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="238"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="296"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="336"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="364"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="389"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="463"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="81"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="522"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="529"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR24692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="542" mass="60094" checksum="4A01356F69AAB974" modified="2009-07-28" version="1">MTRNSFASSSNSVVETEGSKCCVERKPRAQHQVTWAEVQEYLRTRFTTLVPTRQKLREARSKYALNPFPALRHMNARQTQFFIVGFLAWTWDALDFFAVSLNMSAIAKDLDKSIKDMSNAITLVLLLRVIGAVIFGYLGDRYGRKLPYCISMFLIIVVQIGTGFVKTFPAFLGCRAIFGIVMGSIFGVASATALENAPEESRSILSGIFQEGYALGYLLGVIFKRAIVDNSPHGWRSFFWFSAGPPALFIVWRLLLPESEVYTRRMNESKRVAQEQEKTNSSQFWQNVKVALIQHWMTLVYLVILMAMFNFSSHGSQDLFPTMLTNQYNYSDDASTVTNSVANIGALVGGILVAHVSSFIGRRCAIVICCIGGGALLYPWGFVSNPKGLNPAVFFLQFFVQGAWGVVPIHLTELSPVEFRAFVTGVAYQMGNMISSASSTIEATIGERFPIDGKEGVYDYGKVMCIFMGCVFAGLLITTVLGPENKGGELLIDERVLDTRGALNNVGDFDLEHADTFSLPKPSVEQIADVERNSENSDRAPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLQ4</accession>
    <name>C5DLQ4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pre-mRNA-splicing factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0G02618g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in spliceosome maturation and the first step of pre-mRNA splicing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the SPP2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555152.1">
        <property type="nucleotide sequence ID" value="XM_002555106.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLQ4"/>
    <dbReference type="FunCoup" id="C5DLQ4">
        <property type="interactions" value="89"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLQ4"/>
    <dbReference type="GeneID" id="8293415"/>
    <dbReference type="KEGG" id="lth:KLTH0G02618g"/>
    <dbReference type="eggNOG" id="ENOG502S8BR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110336_0_0_1"/>
    <dbReference type="InParanoid" id="C5DLQ4"/>
    <dbReference type="OMA" id="SKIRITH"/>
    <dbReference type="OrthoDB" id="5577072at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045166">
        <property type="entry name" value="Spp2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026822">
        <property type="entry name" value="Spp2/MOS2_G-patch"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15818">
        <property type="entry name" value="G PATCH AND KOW-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15818:SF2">
        <property type="entry name" value="G-PATCH DOMAIN AND KOW MOTIFS-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12656">
        <property type="entry name" value="G-patch_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Spp2/MOS2 G-patch" evidence="6">
        <location>
            <begin position="121"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="11"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU369096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR24715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="218" mass="24043" checksum="5BAF970086D37061" modified="2009-07-28" version="1">MVGFSINLKAGKVSKKTASSEKKKKRANIFNDADANKVPKSKIRITHIDEHQNSAEPQLVIKPPPANKGWNVPIVDENGRDEESLGYGLNQSTSGARDLRRQHNREALALSRKPNSVVHLPTETAQEEYETVPVEEFGDALLRGMGWNGELENEVVAKSAGKSKPVLPHEQFRPSLLGLGAKASQNTVSEGINKSEPFLPIIKVSRRRGKPIEEQQLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLU4</accession>
    <name>C5DLU4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0G03608p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0G03608g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555192.1">
        <property type="nucleotide sequence ID" value="XM_002555146.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLU4"/>
    <dbReference type="STRING" id="559295.C5DLU4"/>
    <dbReference type="GeneID" id="8293458"/>
    <dbReference type="KEGG" id="lth:KLTH0G03608g"/>
    <dbReference type="eggNOG" id="KOG0817">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_950185_0_0_1"/>
    <dbReference type="InParanoid" id="C5DLU4"/>
    <dbReference type="OrthoDB" id="346910at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000062">
        <property type="term" value="F:fatty-acyl-CoA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000582">
        <property type="entry name" value="Acyl-CoA-binding_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035984">
        <property type="entry name" value="Acyl-CoA-binding_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23310">
        <property type="entry name" value="ACYL-COA-BINDING PROTEIN, ACBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23310:SF133">
        <property type="entry name" value="COA BINDING PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G12300)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00887">
        <property type="entry name" value="ACBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47027">
        <property type="entry name" value="Acyl-CoA binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51228">
        <property type="entry name" value="ACB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACB" evidence="2">
        <location>
            <begin position="6"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR24755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="293" mass="33510" checksum="98D73D51EC69FA09" modified="2009-07-28" version="1">MSADSVDQVFEKAISTIHTLSSLKGYNSLPRPPAEIRTELYALFKQSTEGDVDLVLQRPTVGSDLPEYSVALRKWEAWRTKTGLSKTEAKKQYIQKLISTMRSYATGTLAARELLADLEFLWSQVAHENVGDSEESNSMAMALQDDQQDYKSLRLRREIYETLFALNESKASSSRDLDVQNKRYKRDPDRGLPRFRKILRWVTIFVCRKLINLFKAMIFQGTVLLAALVLAKRLNLSPLSIDLNIPRNKNFNTTSKGSRYKALSYIRNGLGAAFEYVNRTLHLNLHHIYISVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLY3</accession>
    <name>C5DLY3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0G04466p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0G04466g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555231.1">
        <property type="nucleotide sequence ID" value="XM_002555185.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLY3"/>
    <dbReference type="FunCoup" id="C5DLY3">
        <property type="interactions" value="56"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLY3"/>
    <dbReference type="GeneID" id="8293497"/>
    <dbReference type="KEGG" id="lth:KLTH0G04466g"/>
    <dbReference type="eggNOG" id="ENOG502QU63">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035923_0_0_1"/>
    <dbReference type="InParanoid" id="C5DLY3"/>
    <dbReference type="OMA" id="LLRCQLF"/>
    <dbReference type="OrthoDB" id="4050598at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014803">
        <property type="entry name" value="DNA_repair_Nse5/Nse6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08691">
        <property type="entry name" value="Nse5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR24794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="541" mass="61621" checksum="DB1B90B02E0CA0BD" modified="2009-07-28" version="1">MSILSHGSEKVGVPYNVGLTTESLSVFEVLNSLCVVENYDQLLYYLEARRSALAVPPADVLIVMMTLSVIPDFGAATLEAEMDPYNVGRASLATRSIKLLRHFTDILLSEHAEEENHYSLALLRSQFFIMLDQLSLRGITSTRVRERDFTKRRKTNSGVLLEASPSIFRDDQSPPLLSFKNPYPSYVSCLEHRPEVLKNMPITSKLSQEGEFWNCVGWALYASLSDDSHIYERSKVWFAVLSVLFDAFEMRNKYSIDSETDPTANNAARLSFIERSPLVALFRSLNSQGLIQAFCDILFLGCSYELAGKLRVHCVYPKELMQVDTYVPKLLARTSCKFTQSMSFRHRLLSLFLTMLRSIPEGVEFSKPCLSEAGFVRHLGKVLSSFEDTSQLKQFFSVTNPYEFLDYMPLFAQAAANESLKAFDANYTLSLVEKLANNDLVIEELGNVFEAGFPPLDENYDSRPEQFYSSIEKCDIFLLVILRYWEYIHGAQLKMTRMRCAIRALSVSDQKRLEFAKLRGWDVLHVPSLHDHFLSVLSISR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5DM04</accession>
    <name>C5DM04_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">KLTH0G04928p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0G04928g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus inner membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555252.1">
        <property type="nucleotide sequence ID" value="XM_002555206.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DM04"/>
    <dbReference type="FunCoup" id="C5DM04">
        <property type="interactions" value="62"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DM04"/>
    <dbReference type="GeneID" id="8293518"/>
    <dbReference type="KEGG" id="lth:KLTH0G04928g"/>
    <dbReference type="eggNOG" id="ENOG502QVG5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010838_0_0_1"/>
    <dbReference type="InParanoid" id="C5DM04"/>
    <dbReference type="OMA" id="KWECGEL"/>
    <dbReference type="OrthoDB" id="2503928at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005637">
        <property type="term" value="C:nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071763">
        <property type="term" value="P:nuclear membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12935">
        <property type="entry name" value="LEM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1180">
        <property type="entry name" value="MAN1, winged-helix domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025856">
        <property type="entry name" value="HeH/LEM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044780">
        <property type="entry name" value="Heh2/Src1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018996">
        <property type="entry name" value="Man1/Src1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041885">
        <property type="entry name" value="MAN1_winged_helix_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47808">
        <property type="entry name" value="INNER NUCLEAR MEMBRANE PROTEIN HEH2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47808:SF2">
        <property type="entry name" value="LEM DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12949">
        <property type="entry name" value="HeH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09402">
        <property type="entry name" value="MSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="408"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="623"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="domain" description="HeH/LEM" evidence="10">
        <location>
            <begin position="12"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="Man1/Src1-like C-terminal" evidence="9">
        <location>
            <begin position="415"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="53"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="53"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="73"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="98"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="114"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="130"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="149"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="160"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="215"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="237"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="250"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="273"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="294"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="333"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="354"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF09402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF12949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR24815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="742" mass="83807" checksum="03582525156475B3" modified="2009-07-28" version="1">MEEDYLGESFEPKSLRVAQLRRVLVEHGVRVQSHLKKAELCELFEKHIKAKREQLLRERESGQVVKIEKATDSSFSHEASSEKSGSPFSDVNDFQNDENARPRKRKIEEESTGRKKSGKKSKKRASAEPARAKKQESKDNTSSPLVNKLRSKSPSKKSPLKPLAIEAFESSSSSSSDAASSSSDDLDFSLRRKAASPDLSKLKVSPEFAKKLAKASSSQGSNRSQSSRGASRESEMETTPQRLSASADKSSVRESSEPKDRSFKDLPSFAPEGKVKGESKEEEPSGASTPTRGPSAQSSHQSSPHLSVPDFKEVAGDAGSEGPSSPDKKFSAEEDETVESSEAEEDEESISVENLSEQQDESNTSKSRSSIKTNIKEAKESAKEFLLEKAKEHPVPQRAWLRALGKGIWNLSVFLMVMIPILFGLWYREERIQIGYCGHEINLPTFENTNDSPLLIALENFLDNHRPQCLPCPENAICYPYMKIKCRPDYVVTQSKWSLQGIFPVSDYCAKDTKRQKLIAEVVRKSLELLRTKNANVKCGEGANDFESGITEEDLYEIFFESRAPSISDEDDIPAESHDLSQQEEHIQPLRKDRLFRSTSKKYIGLKCKFEKEVHQTYQRFKYVIWIAFALTVIIKTVTFKLRSHFKRQQRTEALTQQVVAKLQKSAKESSESRPPYMSTVQLRDVLLSDVVDLKLKNKLWNSVIKKLEHNNTNVKSRLMELHGEIMKCWEWVGPLQEEDQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMC7</accession>
    <name>C5DMC7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0G07788p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0G07788g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555375.1">
        <property type="nucleotide sequence ID" value="XM_002555329.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMC7"/>
    <dbReference type="FunCoup" id="C5DMC7">
        <property type="interactions" value="153"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMC7"/>
    <dbReference type="GeneID" id="8293646"/>
    <dbReference type="KEGG" id="lth:KLTH0G07788g"/>
    <dbReference type="eggNOG" id="KOG1882">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022457_1_3_1"/>
    <dbReference type="InParanoid" id="C5DMC7"/>
    <dbReference type="OMA" id="KCYVMDL"/>
    <dbReference type="OrthoDB" id="444265at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050923">
        <property type="entry name" value="Cell_Proc_Reg/RNA_Proc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23308">
        <property type="entry name" value="NUCLEAR INHIBITOR OF PROTEIN PHOSPHATASE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FHA" evidence="1">
        <location>
            <begin position="111"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="212" mass="24466" checksum="740532C51FBE376F" modified="2009-07-28" version="1">MRQQELGKRGLDLETAGALQPKRFRSQRASDLPRKAILPIFGPSGLLELESHSKKGIQLQHVEPHDSISPNAYFEKYKIPIRKQTHFQALLYSEKDEAYHEKFDLLERSNYLVGRRVREDPESEDEQENKETVLADIPIREETCSKQHCVIQFREREGILKAYVIDLDSSNGTLLNDVALPRARYVELKNEDVLKFSADESDTCYFLIFVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMD4</accession>
    <name>C5DMD4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0G07942p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0G07942g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 15 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555382.1">
        <property type="nucleotide sequence ID" value="XM_002555336.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMD4"/>
    <dbReference type="FunCoup" id="C5DMD4">
        <property type="interactions" value="54"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMD4"/>
    <dbReference type="GeneID" id="8293653"/>
    <dbReference type="KEGG" id="lth:KLTH0G07942g"/>
    <dbReference type="eggNOG" id="KOG4472">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024327_4_1_1"/>
    <dbReference type="InParanoid" id="C5DMD4"/>
    <dbReference type="OMA" id="HWYSCTP"/>
    <dbReference type="OrthoDB" id="439943at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000032">
        <property type="term" value="P:cell wall mannoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000051">
        <property type="entry name" value="Alpha-1,2-mannosyltransferase (Ktr4)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002685">
        <property type="entry name" value="Glyco_trans_15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121">
        <property type="entry name" value="ALPHA-1,2 MANNOSYLTRANSFERASE KTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121:SF6">
        <property type="entry name" value="ALPHA-1,2 MANNOSYLTRANSFERASE KTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01793">
        <property type="entry name" value="Glyco_transf_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018153">
        <property type="entry name" value="Glyco_trans_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR018153-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR24945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="387" mass="44933" checksum="133EB386107772AE" modified="2009-07-28" version="1">MQLNGKDGARRALGAACIVAITLWVLVRGASIGGSSAQRTSTVVKHTDGFKYRKVAQPKYTGPKEKATFVTLARNQDLYSLLGSIASIEDRFNSKFQYDWIFLNDEEFSEEFKRVTTAMVSGQTKYGLVPEEHWSYPSWIDKGKAAAVREEMREKKIIYGDSESYRHMCRYESGFFYRHELLQDYDWYWRVEPDTRIYCDVDYDVFKFMRDNGKKYGFTISLKEYVGTIETLWNTTLEFTEKNPSLVHPNNMMDFISDDGGLSYNLCHFWSNFEVGWLDLWRGPAYSAYFDHLDHAGGFFYERWGDAPVHSIGAALFLDRSEIHHFGDLGYFHIPFHACPIDLNTRLENRCNCDPNQDFTWKGYSCTSKFYTVSGKDRPAGWQKYAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMG1</accession>
    <name>C5DMG1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA mismatch repair protein PMS1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0G08624g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA mismatch repair MutL/HexB family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555409.1">
        <property type="nucleotide sequence ID" value="XM_002555363.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMG1"/>
    <dbReference type="FunCoup" id="C5DMG1">
        <property type="interactions" value="792"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMG1"/>
    <dbReference type="GeneID" id="8293684"/>
    <dbReference type="KEGG" id="lth:KLTH0G08624g"/>
    <dbReference type="eggNOG" id="KOG1978">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004131_0_2_1"/>
    <dbReference type="InParanoid" id="C5DMG1"/>
    <dbReference type="OMA" id="SFNNVQY"/>
    <dbReference type="OrthoDB" id="10263226at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032389">
        <property type="term" value="C:MutLalpha complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000710">
        <property type="term" value="P:meiotic mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16926">
        <property type="entry name" value="HATPase_MutL-MLH-PMS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03484">
        <property type="entry name" value="MutL_Trans_hPMS_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.100:FF:000001">
        <property type="entry name" value="Mismatch repair endonuclease pms1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000014">
        <property type="entry name" value="Mismatch repair endonuclease pms1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1540.20">
        <property type="entry name" value="MutL, C-terminal domain, dimerisation subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.100">
        <property type="entry name" value="MutL, C-terminal domain, regulatory subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014762">
        <property type="entry name" value="DNA_mismatch_repair_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013507">
        <property type="entry name" value="DNA_mismatch_S5_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002099">
        <property type="entry name" value="MutL/Mlh/PMS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038973">
        <property type="entry name" value="MutL/Mlh/Pms-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014790">
        <property type="entry name" value="MutL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042120">
        <property type="entry name" value="MutL_C_dimsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042121">
        <property type="entry name" value="MutL_C_regsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037198">
        <property type="entry name" value="MutL_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00585">
        <property type="entry name" value="mutl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MLH, PMS, MUTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073:SF52">
        <property type="entry name" value="MISMATCH REPAIR ENDONUCLEASE PMS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01119">
        <property type="entry name" value="DNA_mis_repair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13589">
        <property type="entry name" value="HATPase_c_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08676">
        <property type="entry name" value="MutL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01340">
        <property type="entry name" value="DNA_mis_repair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00853">
        <property type="entry name" value="MutL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118116">
        <property type="entry name" value="DNA mismatch repair protein MutL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00058">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair protein S5" evidence="6">
        <location>
            <begin position="216"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="MutL C-terminal dimerisation" evidence="5">
        <location>
            <begin position="709"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="372"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="566"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="372"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="403"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00070941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR24972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="906" mass="101669" checksum="304CB94ACD7798CE" modified="2009-07-28" version="1">MSSKIAAINDADVHRITSGQVIIDLASAVKELLDNSIDSGADQVVCTFKNYGLESLECSDNGSGVPEDSYESLALKHYTSKISSFEDVSQVTTLGFRGEALSSLAAIASLVVTTTTQPPKAARLEYNFKGELVKKTVTSRNKGTTVHVSQLFNNLPVRKKEFTRNHKRQFSKCIMLLQAYTIIQERMKISVWHITGNGRRSLVLSSTKDRGIPKRIIGVFGSSAMQGLSDIHLTLQISPKRSFSSQLSGAEVSSGSPEYIIVVSGYISRNTFGCGRTAKDRQFIYINQRPVIYPSLAKSCNEVYRTHNNVQYPVFVLNFELSPEFIDVNVTPDKRTVLLHAESSVIDAFREALADYYSEQEMVLPISNTHTVKVDNDNTEPKVAKPGVMSQELDGEYNNDVGEDSRQTLDGRIDTGPYRENGPKTESPESEEREALFDLSNAKVSNKMIEKSLPQPNLFVDEPDSPSENNATTAKAPAPEAIVKEVIGTDNEERTAKRAADYELNINKKQKTLDPFVNPSQEQDSDLYLRGENSISQEPITLQIGDKKIEEKAILTRDNRLLFSSNPKAPQSCSCSSDNEESDSESLNQSAITNVIGSERAPSDTGAENSYPIIRPNADSYARKAPKSSWESAAIQKSSNDCVSLTTAITVRLQSIKKSFSHIYQLMESRKGTQHETHYQKNDKLDDFEEGERYLTLSVSKSDFKKMEIVGQFNLGFILVTRRKSGKFDLFIIDQHASDEKYNFEKLQKNTVFKSQKLLAPQIVEMSIIDELVMMDNLEVFEKNGFKLEIDEEQPQGCRVKVVSLPVSRKTLFDMNDLHELIHLVKESDGLSKDSIRCSKIRAMHAMRACRSSIMVGRPLVKKSMLRVVRNLSELDKPWNCPHGRPTMRHLMELRDWDSFNEDYKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DML6</accession>
    <name>C5DML6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase</fullName>
            <ecNumber evidence="3 12">2.4.1.131</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0G09922g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. Catalyzes, on the cytoplasmic face of the endoplasmic reticulum, the addition of the fourth and fifth mannose residues to the dolichol-linked oligosaccharide chain, to produce Man(5)GlcNAc(2)-PP-dolichol core oligosaccharide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>an alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + 2 GDP-alpha-D-mannose = an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + 2 GDP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:29523"/>
            <dbReference type="Rhea" id="RHEA-COMP:19515"/>
            <dbReference type="Rhea" id="RHEA-COMP:19516"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57527"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:132511"/>
            <dbReference type="ChEBI" id="CHEBI:132515"/>
            <dbReference type="EC" id="2.4.1.131"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11 12">
            <dbReference type="Rhea" id="RHEA:29524"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.131" evidence="3 12"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555464.1">
        <property type="nucleotide sequence ID" value="XM_002555418.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DML6"/>
    <dbReference type="FunCoup" id="C5DML6">
        <property type="interactions" value="437"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DML6"/>
    <dbReference type="GeneID" id="8293742"/>
    <dbReference type="KEGG" id="lth:KLTH0G09922g"/>
    <dbReference type="eggNOG" id="KOG1387">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017896_1_1_1"/>
    <dbReference type="InParanoid" id="C5DML6"/>
    <dbReference type="OMA" id="WKHFTLI"/>
    <dbReference type="OrthoDB" id="2276068at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004377">
        <property type="term" value="F:GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03806">
        <property type="entry name" value="GT4_ALG11-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038013">
        <property type="entry name" value="ALG11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031814">
        <property type="entry name" value="ALG11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45919">
        <property type="entry name" value="GDP-MAN:MAN(3)GLCNAC(2)-PP-DOL ALPHA-1,2-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45919:SF1">
        <property type="entry name" value="GDP-MAN:MAN(3)GLCNAC(2)-PP-DOL ALPHA-1,2-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15924">
        <property type="entry name" value="ALG11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="ALG11 mannosyltransferase N-terminal" evidence="14">
        <location>
            <begin position="119"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="13">
        <location>
            <begin position="345"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU367051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF15924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR25027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="573" mass="65268" checksum="37847AA9F4B30B35" modified="2009-07-28" version="1">MSINEVLAIIATALAASVFIAKVLAGFLPHFFRAPPAQWRLRVNKAMMRAENSPNKVPALDFGWKNGSVRRQMIRASYQPSAYTNKMSTQKLVVGTEDRANRESFVNRLAHNLRTFDRKIYGYFHPYCNAGGGGEKVLWKAVETTLQKDPNGVVAIYTGDNDVSGEEILSNVIKRFEYNLDASRIVFIFLTKRDFVDPKKWPRLTLLGQAFGSAVLAAEALFKLTPDVWCDTMGYPFTYPIVHFLVNVPVVTYTHYPVISSDMLQKLVLTPGFKNNLKLRLKYYYWKIFMLLYTFTGKFVTIATTNSTWTQNHISRIWPQKSKRIIYPPCSTENLAFGEDGSKWTRKNQAVIIAQFRPEKRHNLIIRSFGSALKKASAQELRAMPKLIFIGSTRGQNDRNYVETLKTLSYETLGISNDLIEFKTDCEYEEMKKHLRTSSYGINAMWNEHFGIAVVEYIASGLIPLVHASAGPLLDIVVPWDSTQQKQANNATEDNRTGFFFRDPSDPDFSSQHNSEYPTLESLFIKIMSLTNDEKLSITRRGKNCVLNKFSDQKFAEQWRSVLEDIPPADSAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMP8</accession>
    <name>C5DMP8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0G10648p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0G10648g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555496.1">
        <property type="nucleotide sequence ID" value="XM_002555450.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMP8"/>
    <dbReference type="FunCoup" id="C5DMP8">
        <property type="interactions" value="49"/>
    </dbReference>
    <dbReference type="GeneID" id="8293774"/>
    <dbReference type="KEGG" id="lth:KLTH0G10648g"/>
    <dbReference type="eggNOG" id="ENOG502S412">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130042_0_0_1"/>
    <dbReference type="InParanoid" id="C5DMP8"/>
    <dbReference type="OMA" id="RYGRVWP"/>
    <dbReference type="OrthoDB" id="5554402at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019419">
        <property type="entry name" value="AIM19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28177">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 19, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28177:SF1">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 19, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10315">
        <property type="entry name" value="Aim19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR25059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="142" mass="14785" checksum="5161C71D2C5212A5" modified="2009-07-28" version="1">MSASDVRELSSTPYPALANSLMLLATPVVSPAANAPARQAAQGAGWSRFGSFWSRPKLVGPSTKTALLFGAAQAVGSWIIYDGDLESGSGFLGAWSALYLIVGGRGSISALRYGRVWPLALSAAALSNTLLYARRFISGGFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMW0</accession>
    <name>C5DMW0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0G12100p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0G12100g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555558.1">
        <property type="nucleotide sequence ID" value="XM_002555512.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMW0"/>
    <dbReference type="FunCoup" id="C5DMW0">
        <property type="interactions" value="38"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMW0"/>
    <dbReference type="GeneID" id="8293840"/>
    <dbReference type="KEGG" id="lth:KLTH0G12100g"/>
    <dbReference type="eggNOG" id="KOG1677">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060370_0_0_1"/>
    <dbReference type="InParanoid" id="C5DMW0"/>
    <dbReference type="OMA" id="KPCINWS"/>
    <dbReference type="OrthoDB" id="410307at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000001">
        <property type="entry name" value="zinc finger CCCH domain-containing protein 15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000018">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045877">
        <property type="entry name" value="ZFP36-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547">
        <property type="entry name" value="CCCH ZINC FINGER/TIS11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547:SF18">
        <property type="entry name" value="PROTEIN TIS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="176"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="214"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="176"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="214"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="50"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="145"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="58"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR25121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="279" mass="30475" checksum="19F7B722CB0984FA" modified="2009-07-28" version="1">MQAHKLYPGSVSSQSTQTSSVFSAEEEDYDSRIKEIEAYYMRTLLAEDGDGDGGAGAGASAESPTKSTAAQAKPAPGAWASGVWNQAALVSPATAGTASGAGRALEVEINPQYMPATGVMPLTSENLKLLQNPKLAPHKLECVAEKPARKQQSSRKPGEQPAQQQGQPHEKCNKVLYKTELCESFSTKGSCKYGHNCQFAHGLQELKFKERNNKFRTKPCVNWMRTGSCPYGQRCCFKHGSDQDIKVYLQAGHIPRGRDTEPARRNMHADVQALQKMAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>C5DN27</accession>
    <name>C5DN27_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0G13640p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0G13640g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555625.1">
        <property type="nucleotide sequence ID" value="XM_002555579.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DN27"/>
    <dbReference type="FunCoup" id="C5DN27">
        <property type="interactions" value="143"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DN27"/>
    <dbReference type="GeneID" id="8293908"/>
    <dbReference type="KEGG" id="lth:KLTH0G13640g"/>
    <dbReference type="eggNOG" id="KOG0712">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017633_10_0_1"/>
    <dbReference type="InParanoid" id="C5DN27"/>
    <dbReference type="OMA" id="THCFKHL"/>
    <dbReference type="OrthoDB" id="550424at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072655">
        <property type="term" value="P:establishment of protein localization to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10747">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10719">
        <property type="entry name" value="DnaJ_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.230.10:FF:000001">
        <property type="entry name" value="DnaJ subfamily A member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000003">
        <property type="entry name" value="DnaJ subfamily A member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.230.10">
        <property type="entry name" value="Heat shock protein DnaJ, cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.20">
        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044713">
        <property type="entry name" value="DNJA1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001305">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43888">
        <property type="entry name" value="DNAJ-LIKE-2, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00684">
        <property type="entry name" value="DnaJ_CXXCXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="DnaJ/Hsp40 cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51188">
        <property type="entry name" value="ZF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="1 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="J" evidence="7">
        <location>
            <begin position="2"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="CR-type" evidence="8">
        <location>
            <begin position="130"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CR-type" evidence="6">
        <location>
            <begin position="130"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR25188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="414" mass="46221" checksum="6396FBEC3337287D" modified="2009-07-28" version="1">MDPYETLGVAPDATQDEIKKAYRRLALQHHPDKVSDESLRDESEIKFKEVAAAYELLSNEEKRQNYDTYGDTNGFSGDYSGFQDGDFASFFGNFASNRYQDEYEAQSPPSPRTDDAHVPIRLTMQELYNGKTIRFQAKRNVVCNRCEGTGVRRRARHASACSQCEGHGVKQRLRRLGPGFVTREIVQCDKCKGLGKNLRSEDMCKKCHGKRVTPESKNLNVYIPRGSRHGDRVVLKGEADEEPGKIPGDLVFDIAENSASSSLERRGSDLYAKITISLSDALTGFEKEVCTTLDGRLLKLKVPLGQVVRPGNFVRIDNEGWPLDDQGSKFGDMYVQIDIEFPPDHWFSERSDVKSLQNLLPQAVSSSATSDFNASASDPANTETPLKFSIVKSAEELPQYLSEESEPGPQCAQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DN38</accession>
    <name>C5DN38_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transmembrane 9 superfamily member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0G13882g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the nonaspanin (TM9SF) (TC 9.A.2) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555636.1">
        <property type="nucleotide sequence ID" value="XM_002555590.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DN38"/>
    <dbReference type="FunCoup" id="C5DN38">
        <property type="interactions" value="1015"/>
    </dbReference>
    <dbReference type="GeneID" id="8293919"/>
    <dbReference type="KEGG" id="lth:KLTH0G13882g"/>
    <dbReference type="eggNOG" id="KOG1278">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010714_4_1_1"/>
    <dbReference type="InParanoid" id="C5DN38"/>
    <dbReference type="OMA" id="TILITYH"/>
    <dbReference type="OrthoDB" id="1666796at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072657">
        <property type="term" value="P:protein localization to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004240">
        <property type="entry name" value="EMP70"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766:SF111">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02990">
        <property type="entry name" value="EMP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007360974" description="Transmembrane 9 superfamily member" evidence="7">
        <location>
            <begin position="19"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="325"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="387"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="426"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="458"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="489"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="541"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="580"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="618"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="647"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="180"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR25199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="687" mass="77703" checksum="F4A84E453BDCE950" modified="2009-07-28" version="1">MVSAILLLSILWASQVSGFYLPGVAPTTYHEGDAIPLLVNHLTPTMYYQHADEDGNDLGDKESLLYSYDYYYPKFHFCRPEKLEKQRESLGSIIFGDRIYNSPFQIEMLKNKECASLCSESIPADDAKFINKLITNGFFQNWLVDGLPAARKTTDVRTKSEFYTPGFELGYVGIGGSELRMNGQGGSQDGDDSGSHGSTKEVSESDYLDAPPAKRSLAKRKQVSNTKELVKQLETPYFANHFEIEVQYHDRGNGNYRVVGVIVNPYSIKRESPDTCDKTGELLKLSETEATEVHFSYSVKFTPSETVWATRWDKYLHVYDPKIQWFSLINFSLVVVFLSTVMIHRLYVTLTDDLSRYNQINLDDDFQEETGWKLIHGDVFRTPERSLILSVLVGSGAQLFLMAACTIGFALLGLLSPSSRGSLTTVMFILYALFGSFGSYTSMATYKFFGGPYWKVNMLLTPILVPGLLFCVMLALNFFLVVVESAGAIPFGTMCVIVLLWFLFSIPLSVAGSLIARKKCKWDEHPTKTKQIPRQIPFQPWYLKTVPASLIAGIFPFGSIAVELYFIYSSLWFNKIFYMFGFLFVSFLLLTLTTSLITVLLTYYSLCLENWKWQWRGFWIGGAGCALYMFIHAILFTKFRLGGFTTIVLYVGYSLVMSLLSCLITGTVGFLSSLWFVRRIYSSVKVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>C5DN48</accession>
    <name>C5DN48_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0G14124p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0G14124g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555646.1">
        <property type="nucleotide sequence ID" value="XM_002555600.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DN48"/>
    <dbReference type="FunCoup" id="C5DN48">
        <property type="interactions" value="80"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DN48"/>
    <dbReference type="GeneID" id="8293930"/>
    <dbReference type="KEGG" id="lth:KLTH0G14124g"/>
    <dbReference type="eggNOG" id="ENOG502S5A7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120579_0_0_1"/>
    <dbReference type="InParanoid" id="C5DN48"/>
    <dbReference type="OMA" id="MDYSDAF"/>
    <dbReference type="OrthoDB" id="423534at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013714">
        <property type="entry name" value="Golgi_TVP15"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28128">
        <property type="entry name" value="GOLGI APPARATUS MEMBRANE PROTEIN TVP15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28128:SF1">
        <property type="entry name" value="GOLGI APPARATUS MEMBRANE PROTEIN TVP15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08507">
        <property type="entry name" value="COPI_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="34"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="66"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="94"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR25209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="139" mass="15370" checksum="B045D2F42777363A" modified="2009-07-28" version="1">MSGVPPQFFRIAHLAAGSLATVSALSQTSYILKNFPAFLMALYSLVLAIPMIYLEFRVPPNLYRYASFYFSFFGRGLCLILLSLLLSYGGVFKILVSILLFLSGLVYVILEFVPSIQEPENFRPEGSSIAVGEDGDDII</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>C5DN59</accession>
    <name>C5DN59_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="23">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="28" type="ordered locus">KLTH0G14388g</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="28 29" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="29">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15 23">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-L-serine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38567"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:38568"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phosphocholine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38583"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:38584"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66132"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:66133"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a beta-D-glucosyl-(1&lt;-&gt;1')-N-acylsphing-4-enine(out) + ATP + H2O = a beta-D-glucosyl-(1&lt;-&gt;1')-N-acylsphing-4-enine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66036"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:22801"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:66037"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 22">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="23">Membrane</location>
            <topology evidence="23">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 23">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="23"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555657.1">
        <property type="nucleotide sequence ID" value="XM_002555611.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DN59">
        <property type="interactions" value="68"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DN59"/>
    <dbReference type="GeneID" id="8293942"/>
    <dbReference type="KEGG" id="lth:KLTH0G14388g"/>
    <dbReference type="eggNOG" id="KOG0206">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000846_0_1_1"/>
    <dbReference type="InParanoid" id="C5DN59"/>
    <dbReference type="OMA" id="QALRCGR"/>
    <dbReference type="OrthoDB" id="377733at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070867">
        <property type="term" value="C:mating projection tip membrane"/>
        <property type="evidence" value="ECO:0007669"/>
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        </source>
    </evidence>
    <sequence length="1568" mass="176811" checksum="71263187B2A181BC" modified="2009-07-28" version="1">MSEHQDPKIPRPSYEAADNQSPASPFHDRFQFENAENVTLDNSMLDRSGNEPSTAPSSASRGGSIMRKQGQKKRTANRIMFADGDKPMEYEEFSRESDEDDAHSFTGTPKIANSATFGGSDSLDPGSADVKPSSDQGLKRMRWGTKRTKKGTPSVGRAKTLRWAKKNFHNPFEDDRKTDTDDESGAEYDPTNRSSELRFLYYNMPLPEEYLDEEGKPATDYARNKIRTTKYTPLSFFPKNIALQFKNVANVYFLVLIILGFVDMFGVTNPGLQTVPLIVIVVLTAIKDAVEDSQRTILDMEVNNTATHILSGVENPNVSEDHVSAWRRFKKANSRVLFKLIEFCKSTFSKEGRRAAAQRKREEHHAKSARTPRNSLETFDSYRMSGTYGRESLEYDNIGEIVTSDEVTVIDRSMPARTDCKFEKNYWKNVHVGDIIRIHNDDEIPADVLLLSSSDSDGGCYVETKNLDGETNLKVRQSLRCTHRIRNSRDVTRTKFWLESEGPHANLYSYQGNLKWVDSEDGKLKNEPVNINNLLLRGCSLRNTKWAMGLVIFTGAETKIMLNAGSTPTKRSRISRELNFSVVMNFLLLFILCLVAGIVNGIYYRKSGVSRDYFEFGTVAGSPAANGVVSFWVAVILYQSLVPISLYISVEIIKTAQAAFIYGDVLLYNEKLDYPCTPKSWNISDDLGQIEYIFSDKTGTLTQNVMEFKKCTINGISYGRAYTEALAGLRKRQGIDVEKEALEEREAIANDKKVMIKDLEALNPTAEIDPEEITFISKEFVQDLSGANGDYQKGCNEHFMLALALCHSVLVEKSKKNPDKLELKAQSPDEAALVGTAKEVGFAFAGKTKSGLIVEIQGVKKEFEILNILEFNSTRKRMSCIIKLQGTAPGSQPRALLICKGADSVIYSRLKRTGGANEETLLEKTALHLEQYATEGLRTLCIGQRELSWKEYEEWNRQYEIAAASLTEREEEMEKVADSIERNLTLLGGTAIEDRLQDGVPDSIAILGEAGIKLWVLTGDKVETAINIGFSCNLLNSDMELLVIKASGDDVDEVGSPYEIVDSMIKKHLNDKFGLSGSLDELEAAKNEHKPPTGNYGVVIDGEALKLALENDDISRRFLILCKNCRAVLCCRVSPAQKAAVVKLVKESLDVMTLAIGDGSNDVAMIQSADVGIGIAGEEGRQAVMSSDYAIGQFRYLTRLLLVHGRWSYKRLAEMIPSFFYKNAIFTLSLFWYGIYSNYDGAYLFEFTYLMFYNLAFTSLPVIFMGIMDQDVSDVVSLLVPQLYRAGIMRSEWNQTKFWGYMFDGLYQSVICFFFPYLVYYKTGLVTPNGLGLDHRYWVGIIVTTIAALSCNLYVLIHQYRWDWFSSLFIFLSIIIVFGWTGIWSSSTNSGEFYKSAARVYGSPMFWAVMFVGILFCLLPRFTFDVFQKLFFPRDIDIIREFWKEGHFDQYPPDYDPTDPNRPQINKATTTLHSHRIEEGSDFARVAGAGNNMSRDTVYTEEIPMNFINSAESSPEQFHRKSIARMSLERTREQMRASNELDARYSVERARISHELPGVTRAESLMRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DN92</accession>
    <name>C5DN92_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0G15114p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0G15114g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555690.1">
        <property type="nucleotide sequence ID" value="XM_002555644.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DN92"/>
    <dbReference type="FunCoup" id="C5DN92">
        <property type="interactions" value="224"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DN92"/>
    <dbReference type="GeneID" id="8293975"/>
    <dbReference type="KEGG" id="lth:KLTH0G15114g"/>
    <dbReference type="eggNOG" id="KOG0016">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_6_2_1"/>
    <dbReference type="InParanoid" id="C5DN92"/>
    <dbReference type="OMA" id="FQAIMDF"/>
    <dbReference type="OrthoDB" id="2018133at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004165">
        <property type="term" value="F:delta(3)-delta(2)-enoyl-CoA isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000048">
        <property type="entry name" value="3,2-trans-enoyl-CoA isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051053">
        <property type="entry name" value="ECH/Chromodomain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43684">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43684:SF1">
        <property type="entry name" value="ENOYL-COA DELTA ISOMERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR25253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="278" mass="31156" checksum="7C6C8FCCAD0F49A9" modified="2009-07-28" version="1">MSDLTRMKSSKMSYRIDGLFFVITFTDPQTLNSLSGTDFLYLGHLLEIADRCESTYFTVLQSTGRFFSSGADVSNIEKAQAEGKAQPSGMLGKWLSEFVSRNLFVTHTFATHSKVLVCCLNGPAVGLTAAIVMLCDIVFSMNDKVYLLFPFANLALVTEGALSVTLPLKLGYNTANDILMFAKPVKFDKLIERVIVRNYNLTDAETFNAQVLTDLREMTSGLYPQSFTGIKKLLKRSLDTQMKRANVEEVNDALAFWVEGLPQQRFREIGSKKRKHKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DND5</accession>
    <name>C5DND5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Anaphase-promoting complex subunit 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0G16082g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555733.1">
        <property type="nucleotide sequence ID" value="XM_002555687.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DND5"/>
    <dbReference type="FunCoup" id="C5DND5">
        <property type="interactions" value="337"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DND5"/>
    <dbReference type="GeneID" id="8294019"/>
    <dbReference type="KEGG" id="lth:KLTH0G16082g"/>
    <dbReference type="eggNOG" id="KOG1493">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115512_0_0_1"/>
    <dbReference type="InParanoid" id="C5DND5"/>
    <dbReference type="OMA" id="CIVKWTK"/>
    <dbReference type="OrthoDB" id="1681166at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097602">
        <property type="term" value="F:cullin family protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16456">
        <property type="entry name" value="RING-H2_APC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051031">
        <property type="entry name" value="RING-box_E3_Ubiquitin_Ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024991">
        <property type="entry name" value="RING-H2_APC11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11210">
        <property type="entry name" value="RING BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12861">
        <property type="entry name" value="zf-ANAPC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="45"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR25296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="135" mass="15150" checksum="3F2D87AD8FB9EF45" modified="2009-07-28" version="1">MQLEFKKVYPVFSWSWDIPGDDDADREIGDEDLCGICRVSYNGTCPGCKYPGDNCPLVIGECNHNFHVHCIQQWLATPTAKGLCPMCRQQFSLKRGVAINEGQVEKLANLLLQPRPAALAADDQDMMMDQEFAVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNE1</accession>
    <name>C5DNE1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0G16214p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0G16214g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TCP11 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555739.1">
        <property type="nucleotide sequence ID" value="XM_002555693.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNE1"/>
    <dbReference type="FunCoup" id="C5DNE1">
        <property type="interactions" value="48"/>
    </dbReference>
    <dbReference type="GeneID" id="8294025"/>
    <dbReference type="KEGG" id="lth:KLTH0G16214g"/>
    <dbReference type="eggNOG" id="KOG1981">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009875_0_0_1"/>
    <dbReference type="InParanoid" id="C5DNE1"/>
    <dbReference type="OMA" id="NIYRHLY"/>
    <dbReference type="OrthoDB" id="276323at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010737">
        <property type="term" value="P:protein kinase A signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008862">
        <property type="entry name" value="Tcp11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12832:SF11">
        <property type="entry name" value="LD23868P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12832">
        <property type="entry name" value="TESTIS-SPECIFIC PROTEIN PBS13 T-COMPLEX 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05794">
        <property type="entry name" value="Tcp11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="171"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="248"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="75"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="95"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="249"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="275"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR25302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="866" mass="97462" checksum="6E248C721BDB1447" modified="2009-07-28" version="1">MEHTRTQTNTPPTEAVALHQEPRKGSRGPSEPKTSSGSQAGPAATAPVCPGREATKPLECDYLGDVRTSPDHGSITPSQQTELDDLSSQRSGDESTLSTQQLQLQGTQHQGPSDHSMQVPLRDVQSRPPHLKHSLASQGFLQRANDVIFKPFDKETGSFMAQPGDLSANATAESVTAVGPASQKDSNDNNNESHPTAAVCTDINFTQLASFKAAASKNNKFRSHSVPSILHSSLRSIGISTNCGSFTSQQQQQQQQQQQQQQQQQQQQPTMHMNRGIKKKKPSKHSASLRHALLMNQRIQFSSNEMTLQDRIKYMESYPGPMPLPPINLQCLKEIDLQEIVKNPQLRHDIIFDPLLQFRPNLDGERGLKKKQISDKYWYDVENEIFVYSAKPDAFRFDQTRLVPLFNSLKEVLITIVPQKEVHTVQNVLDTELLVQELLRGSLMMTSFADWLAQLFKHHCAPMRDPWVDKMSLKFKESEDEKSIPKLVEGLRLVFQILEAMKLDIANHQIRILRPALLSNAVEFEKQYFQSLMSSNRVDLKSSFKWFSDKCVEYHKSSTSPSSRLKASEVYRLCIRGIISLLSCRKMVREYPTSLSFDHARLILLRADIRQIVCLLVCRLLFKQLVANDTSMDKLTKDHITNSYTNKKLKEEIVSIITDEHGNCRWTKNTMSIAIHLCKTIQDLRQDFLLQRNLGDVTLAHSKSGNSATTQAPQLDNAQIDFAKSWLSKQTQPLSEVYGVLEARVFKQLENSIFNKSGCTQDGSIKQDFIHLCSTMTSNGSSSANVPSVSNSSASIRSLNSSLPVKEALGVERRPGTPQQSTGLASVDLEEFESLFCHLYTLVNFHWSVFGCLYMDFLGDKMEEVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNE9</accession>
    <name>C5DNE9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Alkyl transferase</fullName>
            <ecNumber evidence="4">2.5.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0G16390g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the UPP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.-" evidence="4"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555747.1">
        <property type="nucleotide sequence ID" value="XM_002555701.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNE9"/>
    <dbReference type="FunCoup" id="C5DNE9">
        <property type="interactions" value="420"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNE9"/>
    <dbReference type="GeneID" id="8294033"/>
    <dbReference type="KEGG" id="lth:KLTH0G16390g"/>
    <dbReference type="eggNOG" id="KOG1602">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038505_0_0_1"/>
    <dbReference type="InParanoid" id="C5DNE9"/>
    <dbReference type="OMA" id="FDRRDLW"/>
    <dbReference type="OrthoDB" id="4173905at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904423">
        <property type="term" value="C:dehydrodolichyl diphosphate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045547">
        <property type="term" value="F:ditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyl diphosphate specific] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016094">
        <property type="term" value="P:polyprenol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00475">
        <property type="entry name" value="Cis_IPPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1180.10:FF:000005">
        <property type="entry name" value="Alkyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1180.10">
        <property type="entry name" value="Decaprenyl diphosphate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01139">
        <property type="entry name" value="ISPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001441">
        <property type="entry name" value="UPP_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018520">
        <property type="entry name" value="UPP_synth-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036424">
        <property type="entry name" value="UPP_synth-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00055">
        <property type="entry name" value="uppS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291:SF43">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE COMPLEX SUBUNIT DHDDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01255">
        <property type="entry name" value="Prenyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64005">
        <property type="entry name" value="Undecaprenyl diphosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01066">
        <property type="entry name" value="UPP_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR25310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="284" mass="31634" checksum="5B69834B9568F70B" modified="2009-07-28" version="1">MTESSSFPGASQVLTLCKNIFSRIIKASDNVPQHVGLIMDGNRRWAKLKHVEIKEGHNAGFHSMSRALELCYEAGVSTATVFAFSIENFKRSSAEVDSLMNLARSGIKQVVQNGEMAQKFGIKINVIGDRKLLPDDVLREVEAAETITKDNKRAVLNICFPYTGRDELLHSIKEVVQATQLGDLASSDINEAAIDRHLYTGGSPPVDLLIRTSGVTRLSDFLIWQVSRRDVVIEFLDCLWPDFGSRQMAWILLKFAFSKSYATGETDPEDYDVEARGSPLKKHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNF5</accession>
    <name>C5DNF5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0G16544p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0G16544g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RMD1/sif2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555753.1">
        <property type="nucleotide sequence ID" value="XM_002555707.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNF5"/>
    <dbReference type="FunCoup" id="C5DNF5">
        <property type="interactions" value="127"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNF5"/>
    <dbReference type="GeneID" id="8294040"/>
    <dbReference type="KEGG" id="lth:KLTH0G16544g"/>
    <dbReference type="eggNOG" id="KOG2861">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011220_1_1_1"/>
    <dbReference type="InParanoid" id="C5DNF5"/>
    <dbReference type="OMA" id="HHTHPKL"/>
    <dbReference type="OrthoDB" id="18302at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003734">
        <property type="entry name" value="DUF155"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051624">
        <property type="entry name" value="RMD1/Sad1-interacting"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16255">
        <property type="entry name" value="REQUIRED FOR MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16255:SF15">
        <property type="entry name" value="SPORULATION PROTEIN RMD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02582">
        <property type="entry name" value="DUF155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="379"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="DUF155" evidence="4">
        <location>
            <begin position="183"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR25316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="408" mass="46580" checksum="616CCB64C4AAAB37" modified="2009-07-28" version="1">MSRPEREPLLAATGGAGRSKNSPSADIPIVQGNKLNAPKASKIGAQRTSRTAQKLKLLPEEPFNRPEDLLLGNADRDVYSQVNRITDTPARRDAEKLGKGQRHLLPRVTAYCTSSAYNMKELVRWLKGCKKAHHTHPKLFDECLFTPFIYTDWRGDKRFKDEDVIRLDDEGGEINVSDKQPDLFIFEYGVIVMWGFTEREEKAFLNDVEKFEKEKLAEEDVQVEQFNYYITQSYQPRIYNDFITLRDGSNYMVKLSISHAIAQSVKISLFEELVDNTIEDTQDIPQQIASSGKVSMGKADIMKSIGELFILRININLHGSVLDSPEIMWSEPQLEPIYHATRGYLEINQRVALLNQRLEVISDLLQMLKEQLGHSHEEYLEFIIIVLVGVEVLISLINIVVDLLSEAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNP1</accession>
    <name>C5DNP1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0G18700p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0G18700g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555839.1">
        <property type="nucleotide sequence ID" value="XM_002555793.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNP1"/>
    <dbReference type="FunCoup" id="C5DNP1">
        <property type="interactions" value="52"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNP1"/>
    <dbReference type="GeneID" id="8294134"/>
    <dbReference type="KEGG" id="lth:KLTH0G18700g"/>
    <dbReference type="eggNOG" id="ENOG502QR34">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028833_0_0_1"/>
    <dbReference type="InParanoid" id="C5DNP1"/>
    <dbReference type="OMA" id="DMRYQVH"/>
    <dbReference type="OrthoDB" id="6419443at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021463">
        <property type="entry name" value="Methyltransf_34"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11312">
        <property type="entry name" value="Methyltransf_34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR25402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="318" mass="35771" checksum="B23EC6F93EEE8C64" modified="2009-07-28" version="1">MAKDATKTLPTHHTASLPPQHIVDLFKVCFDPELYKNEQDVLSAAIQSVKTDLYNRDYPAAFNSFEKRTAYCTRWSPSRAIAYASLFSQLRPVREVIKCEKESSSSSNVLCVGGGAGGELIGIASIFTPSIDFNAKYATTKSAVQSKRLTVKLVDIADWSAVVDRLKGTMRDVWLFDHSSDLDVQSLNLNVLKTGASDLGVPSLDLITLLFTTNELFMEDKAGSIRFFQMLNKECSSGCYLLIAESAGSYSHITVGTKKFPIQFLIDTLLLGKRGEESEGQWELVDQNDSIWYRAKDDMDYPLKVENMRFFYRLYRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2B3</accession>
    <name>C5E2B3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">KLTH0H03564p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0H03564g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transient receptor potential (TRP) ion channel family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556036.1">
        <property type="nucleotide sequence ID" value="XM_002555990.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2B3"/>
    <dbReference type="FunCoup" id="C5E2B3">
        <property type="interactions" value="82"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2B3"/>
    <dbReference type="GeneID" id="8294349"/>
    <dbReference type="KEGG" id="lth:KLTH0H03564g"/>
    <dbReference type="eggNOG" id="ENOG502QSVZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010226_0_0_1"/>
    <dbReference type="InParanoid" id="C5E2B3"/>
    <dbReference type="OMA" id="SIMGWVF"/>
    <dbReference type="OrthoDB" id="5212126at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009272">
        <property type="term" value="P:fungal-type cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010308">
        <property type="entry name" value="TRP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040241">
        <property type="entry name" value="TRP_Flc/Pkd2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032800">
        <property type="entry name" value="TRP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31145:SF4">
        <property type="entry name" value="FLAVIN CARRIER PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31145">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_7G01610)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06011">
        <property type="entry name" value="TRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14558">
        <property type="entry name" value="TRP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01320">
        <property type="entry name" value="TRP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950718" evidence="9">
        <location>
            <begin position="20"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="329"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="379"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="405"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="468"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="493"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="523"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="555"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="domain" description="ML-like" evidence="10">
        <location>
            <begin position="21"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="645"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="687"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="713"/>
            <end position="724"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR30174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="818" mass="91745" checksum="5FBE466714098AE9" modified="2009-07-28" version="1">MRIFSAILLALNIAHLVVAKRKLVASSLVACMQNSQLTASGFSVVFDPDDRSLHYSLDMVTEINDYIYAEVDVYAYGFKIISRNIDLCSLSWKQFCPLRPGNVQVESIEYISESLAKQIPGIAYQVPDIDAFVRMNIYKSTDKSQTLACIQAFFSNGKTVSQTGVKWATAVIAGIGLLCSAVLSTFGNSNAASHISANTMSLFLYFQSVAVVSMEHVHRMPPIAAAWSENIIWSMGLIRISFMQRIFRWFVQSTGGNPSLYLTSKTISVLVQRSLEGIQRGLESIRAPPVFKRNADTIIGNSNVMILRGIKRLAYTSNIENTSVVCTGFTFFVLCGYFLAGFIIASKYAIDLCIKAGWIRNTRFWAFRHNWRVILKGSLLRYIYIGFTQLTLLSFWEFTQNDSPALIVIAVLFLIQSLGLMLWAAYRTISFARLSVEQRKNPAAILYGDQRVLDKYGFFYTMFSAKRYWWNCVILVYILLKSIFISFCQASGKTQALAIFIIDLAYFVVLIKFQPYFDRPTNIVNILITTVTLVNSFLFLFFSDLFGQPGRVSSIMGWVFFIMNAAFSLILLILILIFVCMVIFSKNPDLRFKPAKDDRTSFQRVSDSHGKINKSVAAELMALGNTAKNHDENWEEELYNQQKLQKEQSVSGLDYSDEKLTGSRSNSDDQEDATRLTLAEKLKRKLSFKRTKSTKSTSSRARKLANTDDAPVTSLSREPSTSSAPVVKRDYPGVHSRQQSESKNGLIGSNAVENSKLETLAQEDDAPLVDTDNYQAPHSGSVEFGRDESMDSVNVAATANATSSTDLFTNDGSYYNRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2I0</accession>
    <name>C5E2I0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0H05104p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0H05104g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556103.1">
        <property type="nucleotide sequence ID" value="XM_002556057.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2I0"/>
    <dbReference type="FunCoup" id="C5E2I0">
        <property type="interactions" value="893"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2I0"/>
    <dbReference type="GeneID" id="8294417"/>
    <dbReference type="KEGG" id="lth:KLTH0H05104g"/>
    <dbReference type="eggNOG" id="KOG1299">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013933_3_1_1"/>
    <dbReference type="InParanoid" id="C5E2I0"/>
    <dbReference type="OMA" id="VHQLNNA"/>
    <dbReference type="OrthoDB" id="10266265at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.830.10">
        <property type="entry name" value="Syntaxin Binding Protein 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043154">
        <property type="entry name" value="Sec-1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043127">
        <property type="entry name" value="Sec-1-like_dom3a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027482">
        <property type="entry name" value="Sec1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005715">
        <property type="entry name" value="VPS45_Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR30241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="579" mass="65930" checksum="9FC2E8E6F27AC4DB" modified="2009-07-28" version="1">MNLFQVSDFYIDRIVNSKSKSVLNGSLDKPRIKALLLDKDTTGTISMCTTQSELLEHEVYLIDTLENQNRDVMRHLKCLVYAKPTDETIEHLVKELQDPKYGEYQIFFNNTVAKTQLERLAESDDLEVVSKVEEVFQDYQVLNQDLFSLDVRSSKLFSHQMIWEPSGLADTTHSLISLLLSLKVKPLIRYEAGSRLTSKLAKEVMQEIHKNERALFDFPVMDSPPQLLILDRHQDPLTPLLQPWTYQSMINEYIGIKRNVVDLSKVPDIDDSLEQVVLSSKQDPFFHDTMYLNFGELGDKVKQYVSSYKDKTKSNAQINTIEDIKQFIGKFPEFKKLSGNVSKHMAIVSELDRQLQVQHIWDVSEVEQNISAHMGDSQVYQDLKGVLSDERVSGFHKLKLACIYSLRNATSPHMNEISATLAANVSPEELNFFHHFNSVFQTRTKSANTGKEREDLISELAKKFNQKGQHKSDNVFMQHVPDLSAILSDLSKNKLPEDRFPFLEKPGARTFCQDVIIFHVGGTTFEEARIVHEFNSGMAGDGGGKIRTILGGTTILNTREFLNDCREVGQGSTELTDLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2R8</accession>
    <name>C5E2R8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0H07172p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0H07172g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556191.1">
        <property type="nucleotide sequence ID" value="XM_002556145.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2R8"/>
    <dbReference type="FunCoup" id="C5E2R8">
        <property type="interactions" value="106"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2R8"/>
    <dbReference type="GeneID" id="8294508"/>
    <dbReference type="KEGG" id="lth:KLTH0H07172g"/>
    <dbReference type="eggNOG" id="KOG3105">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009537_0_0_1"/>
    <dbReference type="InParanoid" id="C5E2R8"/>
    <dbReference type="OMA" id="IWIILDD"/>
    <dbReference type="OrthoDB" id="125347at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050863">
        <property type="entry name" value="CenT-Element_Derived"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004875">
        <property type="entry name" value="DDE_SF_endonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006600">
        <property type="entry name" value="HTH_CenpB_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19303:SF73">
        <property type="entry name" value="PROTEIN PDC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19303">
        <property type="entry name" value="TRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03184">
        <property type="entry name" value="DDE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03221">
        <property type="entry name" value="HTH_Tnp_Tc5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00674">
        <property type="entry name" value="CENPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51253">
        <property type="entry name" value="HTH_CENPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH CENPB-type" evidence="3">
        <location>
            <begin position="65"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="585"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="588"/>
            <end position="611"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR30329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="796" mass="90681" checksum="8E3E8F8A99ED43AE" modified="2009-07-28" version="1">MGLLSVEQKYNIILMAERHPKWTQLELARWAYEAYQLSSCPSQGTISRLLARKSVFMNSKEHEKDANRLRKPNNILVRRILQEWVSQSIWNGIPISPPIIQDTAQSVWHRIPAEHREGNGSFSYKWITHFLSKMDVNVSSLDEEPPKPPKVWTFEERGTLKEFLCKIPARNLFTLDETFLAYNLPLDYAQYETNQVQRRIEVVTVMLCANLDGSEKMNPLVIGKYENYRSFRNHFSSEPPEGASQLSLGNKMAKKFSLTYYNNRKSWLTSNLFHDWLVWWDKRLVVDNRKIYIVLDDSCSHRIINLRLKNIKLIYTSANSRFLPFNWGVLDEFKTHYRVQQYEALTELQEKIEKKTGQKKILTYDQSLLKMSNAFKLIKVAWDAIPADTIKANWKSSGILPQHMIQLDESVSMAFKKNEALEKKLYEVSERFHCMKQWDYDMLLDLNIENKNTNFLSSEELVQSAIVDEWEPEEKQVSLEDDYGTNGFSLEAFAQVGEVGNDTFGHVEHLSHNPTETLEKNLLVSDVIDRPSEFFPSSVLPINDDQHIENVTGPPRSNSTQIYPAVSAPPAATGYLDDVFSTLPHEPSSANPATSINTHVSSTLPATDSPSPNGIPAADILGIETPALNLGNEFDLDSVNDYLGQSSSNPHEPTFGNQRLVILTTLQTNIDIAKSMGNILKHAEFREVGLSDRSLNELRFSYNNCLKKIRKAKQALSGADKRLRQQRLERQIINQEQSIVSTAASPSSKIHRPVGISPSLEIENSNFFTNFPSNASLDIPPHIQSGTENSSTRRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2S0</accession>
    <name>C5E2S0_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Conserved oligomeric Golgi complex subunit 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Component of oligomeric Golgi complex 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0H07216g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the COG3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556193.1">
        <property type="nucleotide sequence ID" value="XM_002556147.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2S0"/>
    <dbReference type="FunCoup" id="C5E2S0">
        <property type="interactions" value="779"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2S0"/>
    <dbReference type="GeneID" id="8294510"/>
    <dbReference type="KEGG" id="lth:KLTH0H07216g"/>
    <dbReference type="eggNOG" id="KOG2604">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011639_2_0_1"/>
    <dbReference type="InParanoid" id="C5E2S0"/>
    <dbReference type="OMA" id="DEFELWG"/>
    <dbReference type="OrthoDB" id="296793at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017119">
        <property type="term" value="C:Golgi transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032258">
        <property type="term" value="P:cytoplasm to vacuole targeting by the Cvt pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048685">
        <property type="entry name" value="COG3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048320">
        <property type="entry name" value="COG3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007265">
        <property type="entry name" value="COG_su3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13302">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX COMPONENT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13302:SF8">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20671">
        <property type="entry name" value="COG3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04136">
        <property type="entry name" value="COG3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Conserved oligomeric Golgi complex subunit 3 N-terminal" evidence="9">
        <location>
            <begin position="67"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="Conserved oligomeric Golgi complex subunit 3 C-terminal" evidence="10">
        <location>
            <begin position="231"/>
            <end position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF20671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR30331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="732" mass="84184" checksum="9CEF0AE53BD36A5C" modified="2009-07-28" version="1">MTRSRGNSLVQSIATHAPSGKYLSPSLQDDELLGKLRRIGTSYKQIEVRAQPEARCKENKYSKYDQYVTGVDVKVSEYQTVIDQAEKINKQLALTVDKFCAISSETRDFTESTYELFEKFRNLDDLHKSIREYLSYFEALDPIVRGLHHFSSPNIVRKNSFKSKLSKIDESLQFLCEHKEFKDAEAYRIKFKQCMVRCCSLMATYLSNCLRSMFEEVVKSLEGISQSATRDALLYNKFSSIAADFYSVSRELSSRYSSKHHQTYHEELNSILQDCYNEYFRIRIKLLGPLISSQLENKEAQQKNFSLVKDIQDNLLFFTQLCEDEYSLIVQFFPEKEGKKSFNEWLFHLCEPLHDHVREKVLRESDVASLCDAVTLLNKYYQFEENSEEYDAQFRSVQLDKIFEPLLQDVQYRLIFRAQIYVETSIIAYRPTRDAFSINHRKGGLSKIEKGDGIVKSFLDSISESKDEVEEMHSCYPPVTRAIALLSKIYQMVNSSIFDTLAHHIVHDCVESLRAALRLVRESEDNLDVNLSYLKNLLMLRKQVQNFDIQYVCNETYVDFSGLRDFLRSLAHGGLSAASNSVFSLAREGAPKVVKNMVDARSELSVELRNAIKELTETAAHEIVGGCLNCSGVLISDNTQLRKNVEVILPRIYNQMCIFIKDHEVRIHLIDAIQDLVIRAYADYYEDVTQQAENGKIAKEDVSEIMYVDVFADLFNNVASQLEKGGASNSIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2U7</accession>
    <name>C5E2U7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">RNA polymerase II subunit B1 CTD phosphatase RPAP2 homolog</fullName>
            <ecNumber evidence="12">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0H07832g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Putative RNA polymerase II subunit B1 C-terminal domain (CTD) phosphatase involved in RNA polymerase II transcription regulation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 12">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 12">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11 12">Belongs to the RPAP2 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="12"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556220.1">
        <property type="nucleotide sequence ID" value="XM_002556174.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2U7"/>
    <dbReference type="FunCoup" id="C5E2U7">
        <property type="interactions" value="365"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2U7"/>
    <dbReference type="GeneID" id="8294538"/>
    <dbReference type="KEGG" id="lth:KLTH0H07832g"/>
    <dbReference type="eggNOG" id="KOG4780">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086709_0_0_1"/>
    <dbReference type="InParanoid" id="C5E2U7"/>
    <dbReference type="OMA" id="CCKEHYQ"/>
    <dbReference type="OrthoDB" id="2590500at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043175">
        <property type="term" value="F:RNA polymerase core enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008420">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039693">
        <property type="entry name" value="Rtr1/RPAP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007308">
        <property type="entry name" value="Rtr1/RPAP2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038534">
        <property type="entry name" value="Rtr1/RPAP2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14732">
        <property type="entry name" value="RNA POLYMERASE II SUBUNIT B1 CTD PHOSPHATASE RPAP2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14732:SF0">
        <property type="entry name" value="RNA POLYMERASE II SUBUNIT B1 CTD PHOSPHATASE RPAP2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04181">
        <property type="entry name" value="RPAP2_Rtr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51479">
        <property type="entry name" value="ZF_RTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7 12" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RTR1-type" evidence="13">
        <location>
            <begin position="50"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU367080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR30358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="224" mass="26032" checksum="890A97B43AA0A540" modified="2009-07-28" version="1">MSGVNDIKELVLKPYQSHRQLSIKEADTISLELLDLFSQSNCKDKETLKYIANFLTVETYHDLIDERNLNKRCGYPVCNRSQNRVRDPYGGNMMAKKFLQQNNPYAYLSSYCSKFHYRCSQFYEIQLTDEALFVRIGIHLDNQEQNGSQKHEITLLEDLMQSEQPDQQDLMSIVNGIGQLNIEQSQPSSREELEKDLSDWLSQIKIVENESPSIMGDILKEEPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2X9</accession>
    <name>C5E2X9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13 15">DNA replication licensing factor MCM4</fullName>
            <ecNumber evidence="3 15">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">KLTH0H08690g</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 15">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Component of the MCM2-7 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the MCM family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="3 15"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556252.1">
        <property type="nucleotide sequence ID" value="XM_002556206.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2X9"/>
    <dbReference type="FunCoup" id="C5E2X9">
        <property type="interactions" value="1112"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2X9"/>
    <dbReference type="GeneID" id="8294576"/>
    <dbReference type="KEGG" id="lth:KLTH0H08690g"/>
    <dbReference type="eggNOG" id="KOG0478">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000995_7_1_1"/>
    <dbReference type="InParanoid" id="C5E2X9"/>
    <dbReference type="OMA" id="AFFKCNV"/>
    <dbReference type="OrthoDB" id="10251574at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071162">
        <property type="term" value="C:CMG complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097373">
        <property type="term" value="C:MCM core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005656">
        <property type="term" value="C:nuclear pre-replicative complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902975">
        <property type="term" value="P:mitotic DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006267">
        <property type="term" value="P:pre-replicative complex assembly involved in nuclear cell cycle DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006279">
        <property type="term" value="P:premeiotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17755">
        <property type="entry name" value="MCM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.10:FF:000003">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1640.10:FF:000011">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000217">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1640.10">
        <property type="entry name" value="mini-chromosome maintenance (MCM) complex, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008047">
        <property type="entry name" value="MCM_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018525">
        <property type="entry name" value="MCM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027925">
        <property type="entry name" value="MCM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF66">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14551">
        <property type="entry name" value="MCM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21128">
        <property type="entry name" value="WHD_MCM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01660">
        <property type="entry name" value="MCMPROTEIN4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00847">
        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 15" id="KW-0235">DNA replication</keyword>
    <keyword evidence="10 14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 15" id="KW-0347">Helicase</keyword>
    <keyword evidence="7 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="17">
        <location>
            <begin position="494"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="34"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="61"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="77"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="133"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00073498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU368062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAR30390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="909" mass="101346" checksum="DBB503007CF1E098" modified="2009-07-28" version="1">MSDNRSSPAHDGGSSPMAAPPSSLGGSSPPLFYNPSSSSQPDTYESQQRQHAVGSSPFRYPSSSQGHPHATSDTFASQRRSQRSSGFGRFTDQSSEPDLLSSSLSGHASQRSSQRRSDLHASDLSSPRRIVDLDSQPAPSSSSDPVSEAAEPLRIIWGTNVSIQECATNFRNFLMSFKYKYRKVLDQNEDLINDTEDQELYYVKRLNQMMEMGSLNLNLDARNLLSFKPTEKLYYQLMSYPQEIISIMDQTVKDCMVSLVVDSNAESTLDDVESKFYKIRPYNIETKKGMRELNPNDIDKLISVKGLVLRSTPIIPDMKVAFFKCNICDHTTVVEIDRGVIQEPARCPRVACNQQNSMTLVHNRCSFADKQVIKLQETPDTVPDGQTPHSVSLCVYDELVDSCRAGDRIEITGIFRSIPVRASSTQRALRSLYKTYLDVVHVKKVAHDRLGADTSTVEQELMQNELIHADVQEVPKVSDDQIRKIHAVAARDDVYEVLARSIAPSIFELDDIKKGILLQLFGGTNKTFTKGGRYRGDINILLCGDPSTAKSQILQYVHKIAPRGVYASGKGSSAVGLTAYVTRDIDTKQLVLESGALVLSDGGVCCIDEFDKMSDSTRSVLHEVMEQQTISIAKAGIITTLNARTSILASANPIGSRYNPNLPVTENIDLPPPLLSRFDLVYLVLDKVDEATDRDLAKHLTNLYLEDAPANETEGDVLPVELLTTYINYAKQQYAPVITEQAKTELVRAYVTMRKMGDDSRSDEKRITATTRQLESMIRLSEAHAKMRLSQLVELQDVQEAVRLIKSAIKDYATDPKTGKIDMNLVQTGKSVIQRKLQEDLSREMVNILRERPNNTISYNELVRSINEQSQDKVDVTDISEALSRLQQEDKVVVLGEGVRRSIRLNGRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2Z9</accession>
    <name>C5E2Z9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0H09152p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0H09152g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SNAP-25 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556272.1">
        <property type="nucleotide sequence ID" value="XM_002556226.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2Z9"/>
    <dbReference type="FunCoup" id="C5E2Z9">
        <property type="interactions" value="93"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2Z9"/>
    <dbReference type="GeneID" id="8294597"/>
    <dbReference type="KEGG" id="lth:KLTH0H09152g"/>
    <dbReference type="eggNOG" id="KOG3065">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020823_1_0_1"/>
    <dbReference type="InParanoid" id="C5E2Z9"/>
    <dbReference type="OMA" id="LDINVHM"/>
    <dbReference type="OrthoDB" id="18679at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15857">
        <property type="entry name" value="SNARE_SEC9C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15886">
        <property type="entry name" value="SNARE_SEC9N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000048">
        <property type="entry name" value="Protein transport protein SEC9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19305">
        <property type="entry name" value="SYNAPTOSOMAL ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19305:SF9">
        <property type="entry name" value="SYNAPTOSOMAL-ASSOCIATED PROTEIN 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="4">
        <location>
            <begin position="505"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="59"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="529"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="137"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="147"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="197"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR30410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="568" mass="63673" checksum="079F1533329E6B03" modified="2009-07-28" version="1">MGIKRFFKIKPPEEDTPEVNRDNLIEQGIPTKNVNRTKKEKFAAYGKFAKDKAEDKFYAPPGYENFGKSNPPVLDDLNKSEVDSDAGNAYMREPSKNDFDPYARNNFTNSSAAADPYGKQPYDPYGKQPSDPYGKQSSDPYSSYSSNAYGNQPSGRSSGSATPYADPYTQGRGPSAPALNAGNPYGQRPGNPYAGHSSFSSSTAGSAQPSQNPYSMMKSDPYSAGASSTNVPAPQKPNPYVSREASRSSRPSAAPEDDQFDFETSAAAPKIRNPDLNDDEIDLNATLQDEGDDLNGSIHENYAGELGQTQLQQPDDTRGYQTFEDVQRQNQELQQQQEDEEVDEIKQQIRFTKQSSVASTRNTLKMAQEAEMAGTNTLGMLGHQSEKLNNVERNLDLMKMQNRVADDRVAELKKLNRNILAVHVSNPFTSKRRLREAEERIRNQRIEDKLMQKQTNGRLHQSTNRIEGALNGASQDHDTIRQRYQNQAILEKAKRYQFENDEEDDEMELEIDKNLDKVGQISGRLKRLAIAAGDEIDAQQNRVQNIEEDTDNMDIKIHLNTTRLTQIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5E327</accession>
    <name>C5E327_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">KLTH0H09768p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0H09768g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556300.1">
        <property type="nucleotide sequence ID" value="XM_002556254.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E327"/>
    <dbReference type="FunCoup" id="C5E327">
        <property type="interactions" value="346"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E327"/>
    <dbReference type="GeneID" id="8294625"/>
    <dbReference type="KEGG" id="lth:KLTH0H09768g"/>
    <dbReference type="eggNOG" id="KOG0092">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041217_10_2_1"/>
    <dbReference type="InParanoid" id="C5E327"/>
    <dbReference type="OMA" id="DEEGLMW"/>
    <dbReference type="OrthoDB" id="63533at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016050">
        <property type="term" value="P:vesicle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01860">
        <property type="entry name" value="Rab5_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000464">
        <property type="entry name" value="GTP-binding protein ypt5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0636">Prenylation</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="200"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="218"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR30438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="229" mass="25661" checksum="FDB82199E3B6DEA5" modified="2009-07-28" version="1">MGWVKSPIHKRAHQNKRYNTATMLQFKLVLLGDSSVGKSSIVHRFVKDSFDEFRESTIGAAFLSQTIKLEKHPDVTIKFEIWDTAGQERYKSLAPMYYRNANAALIVYDVTQPGSLVKAQSWVEELKNKVGDQDLVICLAGNKVDICDEDATAREVQREDAQLYAQEQGLLFYETSAKTGAGVSAIFQEIGERVYQKKGMEVPSSTQARKPLNVELQRPSTNDSTSCCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3D1</accession>
    <name>C5E3D1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0H12342p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0H12342g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the acetolactate synthase small subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556404.1">
        <property type="nucleotide sequence ID" value="XM_002556358.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3D1"/>
    <dbReference type="FunCoup" id="C5E3D1">
        <property type="interactions" value="367"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3D1"/>
    <dbReference type="GeneID" id="8294741"/>
    <dbReference type="KEGG" id="lth:KLTH0H12342g"/>
    <dbReference type="eggNOG" id="KOG2663">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055003_0_0_1"/>
    <dbReference type="InParanoid" id="C5E3D1"/>
    <dbReference type="OMA" id="CARSGMM"/>
    <dbReference type="OrthoDB" id="2013116at2759"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00055"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00059"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005948">
        <property type="term" value="C:acetolactate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990610">
        <property type="term" value="F:acetolactate synthase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04878">
        <property type="entry name" value="ACT_AHAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.260:FF:000001">
        <property type="entry name" value="Acetolactate synthase, small subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1150">
        <property type="entry name" value="ACT-like. Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004789">
        <property type="entry name" value="Acetalactate_synth_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027271">
        <property type="entry name" value="Acetolactate_synth/TF_NikR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019455">
        <property type="entry name" value="Acetolactate_synth_ssu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039557">
        <property type="entry name" value="AHAS_ACT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054480">
        <property type="entry name" value="AHAS_small-like_ACT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053050">
        <property type="entry name" value="ALS_regulatory_subunit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00119">
        <property type="entry name" value="acolac_sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31242">
        <property type="entry name" value="ACETOLACTATE SYNTHASE SMALL SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31242:SF2">
        <property type="entry name" value="ACETOLACTATE SYNTHASE SMALL SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22629">
        <property type="entry name" value="ACT_AHAS_ss"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10369">
        <property type="entry name" value="ALS_ss_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACT" evidence="6">
        <location>
            <begin position="73"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR30542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="301" mass="33301" checksum="92218BB50F3F0D09" modified="2009-07-28" version="1">MIRTFLRSKAAERVLVRHSSSSTSALTYKQLHKNRISPPLPTLETPSWSAKSAVSSILYETPSPSRLPRKQHVLNCLVQNEPGVLSRISGTLAARGFNIDSLVVCNTEVKDLSRMTIVLQGQDGVIEQARRQIEDLVPVYAVLDYSNADIIKRELVLARVSLLGAEYFEDLIHHHEQTSSEATEGKNETVAQIRSKKFHPSNLPLSEALRLKHEHLASITNLVGNFGGRVVDISETNCIVELSAKPSRISAFLKLVEPFGVLEVARSGMMALPRTPLELESENELENQISEIVDLSQLPPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBA1</accession>
    <name>C5DBA1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0A00858p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0A00858g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551500.1">
        <property type="nucleotide sequence ID" value="XM_002551454.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBA1"/>
    <dbReference type="FunCoup" id="C5DBA1">
        <property type="interactions" value="70"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBA1"/>
    <dbReference type="GeneID" id="8290291"/>
    <dbReference type="KEGG" id="lth:KLTH0A00858g"/>
    <dbReference type="eggNOG" id="ENOG502QT9V">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026579_0_0_1"/>
    <dbReference type="InParanoid" id="C5DBA1"/>
    <dbReference type="OMA" id="GDYWLPT"/>
    <dbReference type="OrthoDB" id="3647690at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070823">
        <property type="term" value="C:HDA1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038609">
        <property type="entry name" value="HDA1_su2/3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021006">
        <property type="entry name" value="Hda2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026216">
        <property type="entry name" value="HDA3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11496">
        <property type="entry name" value="HDA2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02093">
        <property type="entry name" value="HDA1SUBUNIT3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="617"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="637"/>
            <end position="647"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="647" mass="73550" checksum="2D3D167A7146982C" modified="2009-07-28" version="1">MDLAKILDTAPEPAIVDAQTMGVSGDVSGDYWLPAPMCLYQKELSEQIVSLHYSDILKYYETEDYNTDVVLHSMQTMCLNSEYVATHPYLLIDHCVPKSLITKDIPNHLAETSGKFAVLKDLITLIQDYETETAIVCRSGRTMDLLEALLLGNRVNIKRYRSPSIKTKQKARKHSCTCHLFPSDGFDETSGLPVDPKIYFDLLLCLDPTVDTSMPHIQQILTQARPSSGPGKQAPIVRLTTINAIDHCELYHSKLLDPKSREYLVRVSAAVVVLRDVVGTLPPDLRPIYSQGLKYLVEWLEDPSIPWPLPDIYPIKTFTPMDVEKSLLTEVRFNQANENLAHVFNNGKKRGRKPNSAYSKEGEPISYYQSKRLKNDYSTNPLKQNMAQLTGIATINDGGSVNYHLSGGLLTHKLIQSIGEAYYNLDRQSAELQNFETKEQNQVHRLELVMKEHEDIKNQLEPAIKAQDYNREESTRLLAVVEEEHKKVASSMEELDTYTETLREKSPDFSKLIDCLLEIAKVQEEIEKQQKLKVSRNNEHTYMTEELRRANEALAGFQAEKAPIAEETQQLEQKIEEKLAEDRIQSQKSQERIQKLMAQINDEKAILETLDKQVASMTEQLKKTPPSKVRSSNNAQRGKHRKHSGNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBV9</accession>
    <name>C5DBV9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Phospholipid:diacylglycerol acyltransferase</fullName>
            <ecNumber evidence="9">2.3.1.158</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Triacylglycerol synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0A05742g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a glycerophospholipid + a 1,2-diacyl-sn-glycerol = a monoacylglycerophospholipid + a triacyl-sn-glycerol</text>
            <dbReference type="Rhea" id="RHEA:14057"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:64615"/>
            <dbReference type="ChEBI" id="CHEBI:136912"/>
            <dbReference type="ChEBI" id="CHEBI:136913"/>
            <dbReference type="EC" id="2.3.1.158"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.158" evidence="9"/>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551708.1">
        <property type="nucleotide sequence ID" value="XM_002551662.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBV9"/>
    <dbReference type="FunCoup" id="C5DBV9">
        <property type="interactions" value="268"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBV9"/>
    <dbReference type="GeneID" id="8290512"/>
    <dbReference type="KEGG" id="lth:KLTH0A05742g"/>
    <dbReference type="eggNOG" id="KOG2369">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016065_1_0_1"/>
    <dbReference type="InParanoid" id="C5DBV9"/>
    <dbReference type="OMA" id="FYFLKWV"/>
    <dbReference type="OrthoDB" id="190846at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097038">
        <property type="term" value="C:perinuclear endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046027">
        <property type="term" value="F:phospholipid:diacylglycerol acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019915">
        <property type="term" value="P:lipid storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000160">
        <property type="entry name" value="Phospholipid:diacylglycerol acyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003386">
        <property type="entry name" value="LACT/PDAT_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11440">
        <property type="entry name" value="LECITHIN-CHOLESTEROL ACYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02450">
        <property type="entry name" value="LCAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="17"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="31"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00051335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00066405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00071325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00076553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR21266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="633" mass="71522" checksum="2EF53241FEAB8EF9" modified="2009-07-28" version="1">MGVKNRRKRQESSPESVTDKGQEKRHGAAASHRHVHKKSKKSSQESKKTWRESRRIVFSLGAILGLLIAVYAGAYGGKTNNTLFDNFVNFDSLQDYVDDWKEILPQGLMSLISDAQRTTSPKDLTESFAVGKQLHKDMNLTDKHPVIMVPGVISTGIESWGLYGDEECSSEAHFRKRLWGSFYMLKTMVLDKVCWLRHVMLDPETGLDPANFTLRAAQGFEAADFFMAGYWIWNKVLQNLGAIGYDPNKMATAAYDWRLAYLDLERRDSYFSKLKQKIEMDYKLTGEKTVLVGHSMGSQVVFYFLKWVEAEGPLYGNGGVGWVEKYVDSFVNVAGTLLGAPKTVPALISGEMKDTIQLNALAMYGLEKFFSRKERVDMIQTWGGVPSMLPKGGSMIWGTNEVSVEDNTHNNSHSFGEFIRFERESQSVFSQRSFSMDDSIDLLLRLSPTWLQKRIKDQYSFGVATSEKQMRENELHHTYWSNPLEVPLPDAPSLKIYCIYGVGNPTERAYVYKEDPEHPDLNITIDYESSNPVSFTDGDGTVPVVTHAMCQKWAQGRSPYNPSNASVKIIEIKHQPERFDIRGGAKSAEHVDILGSAELNEYVLKIAGGKGDSIEPRELTNLSQWVRDMPFPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBX5</accession>
    <name>C5DBX5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0A06094p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0A06094g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551724.1">
        <property type="nucleotide sequence ID" value="XM_002551678.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBX5"/>
    <dbReference type="FunCoup" id="C5DBX5">
        <property type="interactions" value="28"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBX5"/>
    <dbReference type="MEROPS" id="S08.A50"/>
    <dbReference type="GeneID" id="8290528"/>
    <dbReference type="KEGG" id="lth:KLTH0A06094g"/>
    <dbReference type="eggNOG" id="KOG1153">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011263_1_0_1"/>
    <dbReference type="InParanoid" id="C5DBX5"/>
    <dbReference type="OMA" id="ANACWSS"/>
    <dbReference type="OrthoDB" id="206201at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030435">
        <property type="term" value="P:sporulation resulting in formation of a cellular spore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd04077">
        <property type="entry name" value="Peptidases_S8_PCSK9_ProteinaseK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000007">
        <property type="entry name" value="Subtilisin-like serine protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034193">
        <property type="entry name" value="PCSK9_ProteinaseK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050131">
        <property type="entry name" value="Peptidase_S8_subtilisin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806">
        <property type="entry name" value="PEPTIDASE S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806:SF13">
        <property type="entry name" value="SUBTILASE-TYPE PROTEINASE RRT12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5 6" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0720">Serine protease</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002947753" evidence="8">
        <location>
            <begin position="20"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="9">
        <location>
            <begin position="182"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="102"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="104"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="184"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="215"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR21282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="496" mass="53282" checksum="D056EBA87E777C8C" modified="2009-07-28" version="1">MRTSLIGALVATSASLAQAEEFLVRLKAPRTLAKAVESRISDGILDVFSEGKILRTFSFGKFEGLTVDFPLSLVSKLRGNPLIAEIVPNVEFNVFDAVEGLEGWGDDEDEDGDYDDDNEEGEDEGDDDVLSIVAQKKAPRHLARISRRTALPYDPENISVPATNFSYYYDDEFSGTCVNAYVIDTGIYKEHPQLEGRAKFGADLVGEGPGDFNGHGTHVAGLIGSRKFGVAKDVSLIEVKALNAKGQGNLTSVISAIEFTVNHCRKSRRGCVANLSLGSFRNTVLNQAVEAALEAGVVIVVAAGNSNANACWSSPASSLSAITVGAFDDRTDTIARFSNWGNCVDIFAPGVKIESLSNVPPFKPVAFSGTSMASPIVSGMVANLLDSGIHPFDIKTELIDLATDSVFHKRTLLFKPGTPNRVAFTGIDKTDDVYEDAVYPSVDIDRLVKDLQSYNSDGHVAEDDATLPLGEIKIKKRSQIVTTLAPPHLSPHSQLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBZ2</accession>
    <name>C5DBZ2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0A06534p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0A06534g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551741.1">
        <property type="nucleotide sequence ID" value="XM_002551695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBZ2"/>
    <dbReference type="FunCoup" id="C5DBZ2">
        <property type="interactions" value="442"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBZ2"/>
    <dbReference type="GeneID" id="8290548"/>
    <dbReference type="KEGG" id="lth:KLTH0A06534g"/>
    <dbReference type="eggNOG" id="KOG2294">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020432_0_0_1"/>
    <dbReference type="InParanoid" id="C5DBZ2"/>
    <dbReference type="OMA" id="DVYSVWK"/>
    <dbReference type="OrthoDB" id="5954824at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00059">
        <property type="entry name" value="FH_FOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000260">
        <property type="entry name" value="Forkhead transcription factor (Sep1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050211">
        <property type="entry name" value="FOX_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018122">
        <property type="entry name" value="TF_fork_head_CS_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030456">
        <property type="entry name" value="TF_fork_head_CS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11829:SF343">
        <property type="entry name" value="FORK-HEAD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11829">
        <property type="entry name" value="FORKHEAD BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00657">
        <property type="entry name" value="FORK_HEAD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00658">
        <property type="entry name" value="FORK_HEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fork-head" evidence="6">
        <location>
            <begin position="108"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="4">
        <location>
            <begin position="108"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="525"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="72"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="525"/>
            <end position="536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR21299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="558" mass="62099" checksum="E281DC441062C1FF" modified="2009-07-28" version="1">MEASAGTAVVGVEHSKRRRDSADAAEPPTLTPPHSTLRKAGTPVKRGKNQHPLSPELSSPIRPHKTRKSKSDTPLITTRSSNSQTLEELIEAFTDRKRLLLLQDPNKKPPFSYAMLIGLAILQSTEVRLTLSQIYQWITYHFPYYKLGDYGWQNSIRHNLSLNEAFVKGEKAPDGKGHYWQVKSGCEGKFFKSGQTDDEVRTKLRKLCSDVLAVTSNPRSPEPELLGKSLYDEGTTTEVERRRLGGRKLDDVYALDISSDEDESFQEQSINNLSRVASSPVGLREALLPFEQASANKEAINQDAFIGELSPAIPYSENDAGASETKNETAFPSLRKSYSADCPQQELWSAPSLTLSPKKDAKKYTCSFNANFEASPHTRTPKTGPLLEGFGSPGSELEGVDFTGPLDLLKTPQLKHANNHEFSYTPRGNTSMKKWRTPTVLFQDFSRSPVLMNSAGPPMMVIDDCVSEEVSCRKPDPFQLGKFCSLPLCESQTHSHKFFENIRYSSGLFGVDVCSVWKRAVEGSRETADRSGKETLLDTPFQGTSRRASTSKEDRVYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBZ9</accession>
    <name>C5DBZ9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0A06688p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0A06688g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the SIMIBI class G3E GTPase family. ZNG1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551748.1">
        <property type="nucleotide sequence ID" value="XM_002551702.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBZ9"/>
    <dbReference type="FunCoup" id="C5DBZ9">
        <property type="interactions" value="322"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBZ9"/>
    <dbReference type="GeneID" id="8290555"/>
    <dbReference type="KEGG" id="lth:KLTH0A06688g"/>
    <dbReference type="eggNOG" id="KOG2743">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017452_6_0_1"/>
    <dbReference type="InParanoid" id="C5DBZ9"/>
    <dbReference type="OMA" id="GHSHMDP"/>
    <dbReference type="OrthoDB" id="258627at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03112">
        <property type="entry name" value="CobW-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1220.10">
        <property type="entry name" value="CobW-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036627">
        <property type="entry name" value="CobW-likC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011629">
        <property type="entry name" value="CobW-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003495">
        <property type="entry name" value="CobW/HypB/UreG_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051316">
        <property type="entry name" value="Zinc-reg_GTPase_activator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13748">
        <property type="entry name" value="COBW-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13748:SF31">
        <property type="entry name" value="ZINC-REGULATED GTPASE METALLOPROTEIN ACTIVATOR 1A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02492">
        <property type="entry name" value="cobW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07683">
        <property type="entry name" value="CobW_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00833">
        <property type="entry name" value="CobW_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90002">
        <property type="entry name" value="Hypothetical protein YjiA, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CobW C-terminal" evidence="8">
        <location>
            <begin position="307"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR21306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="410" mass="45055" checksum="B10F49A2C8CDAC77" modified="2009-07-28" version="1">MSALKDFSYKEDEDGDIPSLIEGNEENLSDILNSIKSDGGGTIVSDKKIELANTQIEEAGSSEAPRVPVTIITGYLGSGKSTLLEKIALKGSDKKIAVILNEFGDSSEIEKSMTIRNGENSYEEWLDLGNGCLCCSLQDVGVKAIESLIARCPGKIDYIILETSGIADPRPIAKMFWQDDGLYSNVYLDGIVTVLDAENILTCLDDVSPATHWHGEKVVTDDNITIAHLQVALADCILINKIDRLGAAANQDAVREIENRVRQINAAASLHHTSFGEVSLDKVLDLHAFESVKFEGSSDSTMHDPRIRTVTLTFRALKDEDEFQRFLDGFLRTVHWKGFGHDLEGDWEIQRTKGLIIVGDNCKVVQGVRETFDVLPGKRLDSAVSCKMVFIGKGISREALLQILKTVIEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCA1</accession>
    <name>C5DCA1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">3-hydroxyanthranilate 3,4-dioxygenase</fullName>
            <ecNumber evidence="9">1.13.11.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">3-hydroxyanthranilate oxygenase</fullName>
            <shortName evidence="9">3-HAO</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">3-hydroxyanthranilic acid dioxygenase</fullName>
            <shortName evidence="9">HAD</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Biosynthesis of nicotinic acid protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">BNA1</name>
        <name evidence="10" type="ordered locus">KLTH0B01364g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 9">Catalyzes the oxidative ring opening of 3-hydroxyanthranilate to 2-amino-3-carboxymuconate semialdehyde, which spontaneously cyclizes to quinolinate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>3-hydroxyanthranilate + O2 = (2Z,4Z)-2-amino-3-carboxymuconate 6-semialdehyde</text>
            <dbReference type="Rhea" id="RHEA:17953"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:36559"/>
            <dbReference type="ChEBI" id="CHEBI:77612"/>
            <dbReference type="EC" id="1.13.11.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="9">Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from L-kynurenine: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the 3-HAO family.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.6" evidence="9"/>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551850.1">
        <property type="nucleotide sequence ID" value="XM_002551804.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCA1"/>
    <dbReference type="FunCoup" id="C5DCA1">
        <property type="interactions" value="179"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCA1"/>
    <dbReference type="GeneID" id="8290677"/>
    <dbReference type="KEGG" id="lth:KLTH0B01364g"/>
    <dbReference type="eggNOG" id="KOG3995">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095765_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCA1"/>
    <dbReference type="OMA" id="KPPVGNQ"/>
    <dbReference type="OrthoDB" id="204928at2759"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00330"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000334">
        <property type="term" value="F:3-hydroxyanthranilate 3,4-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034354">
        <property type="term" value="P:'de novo' NAD+ biosynthetic process from L-tryptophan"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043420">
        <property type="term" value="P:anthranilate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006569">
        <property type="term" value="P:L-tryptophan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019805">
        <property type="term" value="P:quinolinate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06123">
        <property type="entry name" value="cupin_HAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000093">
        <property type="entry name" value="3-hydroxyanthranilate 3,4-dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00825">
        <property type="entry name" value="3_HAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010329">
        <property type="entry name" value="3hydroanth_dOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03037">
        <property type="entry name" value="anthran_nbaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15497">
        <property type="entry name" value="3-HYDROXYANTHRANILATE 3,4-DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15497:SF1">
        <property type="entry name" value="3-HYDROXYANTHRANILATE 3,4-DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06052">
        <property type="entry name" value="3-HAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="8 9" id="KW-0408">Iron</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 9" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>O2</name>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR21412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="191" mass="22081" checksum="684138AAA02136CD" modified="2009-07-28" version="1">MSSINQVKAYLHSFQMLNTTPINIEKWLQENGHLLQPPVNNFCLHRGGFTVMIVGGPNERTDYHVNPTPEWFHQKKGFMTLRVVDETLQGNERFKDVTIEEGDSFLLPANVPHNPVRYANTVGIVVEQDRPEGQHDMLRWYCSNCREVVCQFDFQMLDLGTQVKEGIQKFDSDKEYRICKKCGTENFSSPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCA9</accession>
    <name>C5DCA9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0B01562p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0B01562g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551858.1">
        <property type="nucleotide sequence ID" value="XM_002551812.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCA9"/>
    <dbReference type="FunCoup" id="C5DCA9">
        <property type="interactions" value="210"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCA9"/>
    <dbReference type="GeneID" id="8290686"/>
    <dbReference type="KEGG" id="lth:KLTH0B01562g"/>
    <dbReference type="eggNOG" id="KOG3772">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107716_1_0_1"/>
    <dbReference type="InParanoid" id="C5DCA9"/>
    <dbReference type="OMA" id="RGGFTQW"/>
    <dbReference type="OrthoDB" id="102559at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10828:SF38">
        <property type="entry name" value="ARSENICAL-RESISTANCE PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10828">
        <property type="entry name" value="M-PHASE INDUCER PHOSPHATASE DUAL SPECIFICITY PHOSPHATASE CDC25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="1">
        <location>
            <begin position="28"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="149" mass="16630" checksum="892B6256CCD32DFC" modified="2009-07-28" version="1">MNSRSVAAIRYVDAAELFQWIKRGTTGAGEPFQVIDVRGSDYIGGHIVNGWNYPYRELRDGGARMEELMQRLRSAAGAAGAQPINCVFHCAQSQQRGPSAAMKFLRSVADEDLARMRVCVLRGGFNHWQAEYGEDASVTEAYVPDLWRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCD9</accession>
    <name>C5DCD9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0B02266p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0B02266g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551888.1">
        <property type="nucleotide sequence ID" value="XM_002551842.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCD9"/>
    <dbReference type="FunCoup" id="C5DCD9">
        <property type="interactions" value="156"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCD9"/>
    <dbReference type="GeneID" id="8290718"/>
    <dbReference type="KEGG" id="lth:KLTH0B02266g"/>
    <dbReference type="eggNOG" id="ENOG502QVMS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_842157_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCD9"/>
    <dbReference type="OMA" id="KYAMRQF"/>
    <dbReference type="OrthoDB" id="10052321at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021036">
        <property type="entry name" value="Ribosomal_mS45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28158">
        <property type="entry name" value="37S RIBOSOMAL PROTEIN S35, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28158:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12298">
        <property type="entry name" value="Bot1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="342" mass="38867" checksum="EEF0B5331D30F4F2" modified="2009-07-28" version="1">MLSPARSGPALQQVQLQQVRFISRRRTAYPFYAFKRLGKQHPKKHDSNLKYAMRQFLGPKNFKGEYVYNKYYGVPQNHEPNYIMPEIERGQSLKDNVTGEQLAIQTDGSIAPARVGAGRPRDPVSGKRKLQPFPSNRHCLTNRVLGEELKLQVHDDIQNKHLSAQEVSQKYGLKIPRVEAAVKLVELEQKWEKYNRIAPSLKSMSSVLYKMFPLFVPRVEVSRENLSEIPVPAKALRSRFMTIAESEPFGPIDAAKALELEPAAETLEKLATLGEHSAHHASRSSAHKEKVIYGELLKGEKSLFKFENKHVGKVGFRYGSGNRDSKKDRKIGFNELGKMVYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCM1</accession>
    <name>C5DCM1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0B04224p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0B04224g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551970.1">
        <property type="nucleotide sequence ID" value="XM_002551924.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCM1"/>
    <dbReference type="FunCoup" id="C5DCM1">
        <property type="interactions" value="79"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCM1"/>
    <dbReference type="GeneID" id="8290807"/>
    <dbReference type="KEGG" id="lth:KLTH0B04224g"/>
    <dbReference type="eggNOG" id="ENOG502S56F">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116422_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCM1"/>
    <dbReference type="OMA" id="ECRANRQ"/>
    <dbReference type="OrthoDB" id="4031914at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR21532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="202" mass="23867" checksum="B57E578C72B0BF3A" modified="2009-07-28" version="1">MENSADSFEYLLHLTKGLSTECRSTRQGTERIEHLVKRLAKLTQTSYEELSKNPGPQVLQKYRELGRESERDRLERENYALIYQIERQEYVCRRIWSLIDQVEDLLESIKKFVVEQQGHRLRTENEFLETVVHSRMANLQVNTEDLVEAKNASRTKLDLLTRELKDLCKQIDWNKMSDSEDATLLSRKVSEVESKYKLKLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCN8</accession>
    <name>C5DCN8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 13">Nuclear fusion protein KAR5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12 13">Karyogamy protein 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0B04598g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 13">Required for nuclear membrane fusion during karyogamy.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Nucleus membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the KAR5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551987.1">
        <property type="nucleotide sequence ID" value="XM_002551941.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCN8"/>
    <dbReference type="FunCoup" id="C5DCN8">
        <property type="interactions" value="44"/>
    </dbReference>
    <dbReference type="GeneID" id="8290824"/>
    <dbReference type="KEGG" id="lth:KLTH0B04598g"/>
    <dbReference type="eggNOG" id="ENOG502QVCQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042075_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCN8"/>
    <dbReference type="OMA" id="LSICEFQ"/>
    <dbReference type="OrthoDB" id="5311848at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000742">
        <property type="term" value="P:karyogamy involved in conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048288">
        <property type="term" value="P:nuclear membrane fusion involved in karyogamy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007292">
        <property type="entry name" value="Nuclear_fusion_Kar5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28012">
        <property type="entry name" value="NUCLEAR FUSION PROTEIN KAR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28012:SF1">
        <property type="entry name" value="NUCLEAR FUSION PROTEIN KAR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04163">
        <property type="entry name" value="Tht1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 13" id="KW-0415">Karyogamy</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13 14" id="KW-0732">Signal</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002948666" description="Nuclear fusion protein KAR5" evidence="14">
        <location>
            <begin position="21"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="491"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU368082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR21549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="509" mass="58132" checksum="938043B1A4C22BC1" modified="2009-07-28" version="1">MGASVIVVILWAFYFRLGNSDRLEIAGYKLLSLDDSDSLTDGLERIVELNFPLYKSSCAMNALRDFLPKCIKHGIDTIDPKQRVNAAIKLSFCEFQESGLDELPESCQKSTQDGLKKCLDEMKSSAQWWTTYSGNYQRLPTLCYESSLPFEKEQLLDVFLNVTRMYANFNEVLDLKLQRRFEEYEAAAEKNLFKVERIFEEYFSDFDNSYSARKAMFFQDFEHYQKDVLLAFDKNVKVVQREVGSFESDIWQEVIALKQYIQEMNAELNASGPRDQIKSLKVESLIQIQALRDSIRLASEDSISLLKSKDKSFNDFYSKTTEQLSKLDEALGNTYMDALVVIQDLRGLVQNSITPLLREDIEKPLSQFSQNLAQNLEYLDENFCAKAELWVRSFDGTFQRVHADLNLTAQEVLHINEDLGNFTTTFESHLKALNISVKVFSASMSSLARLLRVVPPAARPFLAIALLRYCPTLLIRFFGSLFENVTQVSRVCAVLIALISGCALGFNLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCQ2</accession>
    <name>C5DCQ2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein transport protein Sec61 subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0B04928g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Necessary for protein translocation in the endoplasmic reticulum.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the SEC61-beta family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552001.1">
        <property type="nucleotide sequence ID" value="XM_002551955.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCQ2"/>
    <dbReference type="FunCoup" id="C5DCQ2">
        <property type="interactions" value="160"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCQ2"/>
    <dbReference type="GeneID" id="8290839"/>
    <dbReference type="KEGG" id="lth:KLTH0B04928g"/>
    <dbReference type="eggNOG" id="KOG3457">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_133423_1_1_1"/>
    <dbReference type="InParanoid" id="C5DCQ2"/>
    <dbReference type="OMA" id="SSGMWRF"/>
    <dbReference type="OrthoDB" id="5401193at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005784">
        <property type="term" value="C:Sec61 translocon complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030671">
        <property type="entry name" value="Sec61-beta/Sbh"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016482">
        <property type="entry name" value="SecG/Sec61-beta/Sbh"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13509">
        <property type="entry name" value="SEC61 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03911">
        <property type="entry name" value="Sec61_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006398">
        <property type="entry name" value="Sec61_beta_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="28"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR006398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR21563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="89" mass="9584" checksum="141F98F785AB6B5B" modified="2009-07-28" version="1">MSTPTPPGGQRTLQKRRQAQSVKEKQQKQTPASTRQAGFGGSSNSVLKLYTDEANGLRVDPLVVLFLAVGFIFSVVALHVVAKVTGKLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDA6</accession>
    <name>C5DDA6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0B09614p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0B09614g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552205.1">
        <property type="nucleotide sequence ID" value="XM_002552159.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDA6"/>
    <dbReference type="FunCoup" id="C5DDA6">
        <property type="interactions" value="311"/>
    </dbReference>
    <dbReference type="GeneID" id="8291048"/>
    <dbReference type="KEGG" id="lth:KLTH0B09614g"/>
    <dbReference type="eggNOG" id="ENOG502S2VH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070379_1_0_1"/>
    <dbReference type="InParanoid" id="C5DDA6"/>
    <dbReference type="OMA" id="QQFQDIH"/>
    <dbReference type="OrthoDB" id="551302at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007062">
        <property type="entry name" value="PPI-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12398:SF20">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY INHIBITOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12398">
        <property type="entry name" value="PROTEIN PHOSPHATASE INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04979">
        <property type="entry name" value="IPP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="147"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="168"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="205" mass="23154" checksum="32908C5D416D086C" modified="2009-07-28" version="1">MVGILKNPLPDRNGESEEPESISEFRKQVYKNTQLNAKLTSKAQNNGKSTSKANAASPKRPVVPRDTLSLKHQHDTLSQAEEEKLKWNQQNLAENDIAKLEYKDINIDEPKTPYQGAVDPTGEYYRDDDDELESLSLGEPVVEMPQSDSDNSSSSGPSDAEPSAEESAEAKHRRFEELRKKHYNVKEALKAQRQNLDDEELEDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDF2</accession>
    <name>C5DDF2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Uridylate kinase</fullName>
            <shortName evidence="9">UK</shortName>
            <ecNumber evidence="9">2.7.4.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">ATP:UMP phosphotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Deoxycytidylate kinase</fullName>
            <shortName evidence="9">CK</shortName>
            <shortName evidence="9">dCMP kinase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Uridine monophosphate kinase</fullName>
            <shortName evidence="9">UMP kinase</shortName>
            <shortName evidence="9">UMPK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">URA6</name>
        <name evidence="11" type="ordered locus">KLTH0C00528g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the phosphorylation of pyrimidine nucleoside monophosphates at the expense of ATP. Plays an important role in de novo pyrimidine nucleotide biosynthesis. Has preference for UMP and dUMP as phosphate acceptors, but can also use CMP, dCMP and AMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>UMP + ATP = UDP + ADP</text>
            <dbReference type="Rhea" id="RHEA:24400"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="9">Binds 1 Mg(2+) ion per monomer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Nucleus</location>
        </subcellularLocation>
        <text evidence="9">Predominantly cytoplasmic.</text>
    </comment>
    <comment type="domain">
        <text evidence="9">Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon ATP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent ATP hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the adenylate kinase family. UMP-CMP kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.14" evidence="9"/>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552251.1">
        <property type="nucleotide sequence ID" value="XM_002552205.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDF2"/>
    <dbReference type="FunCoup" id="C5DDF2">
        <property type="interactions" value="710"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDF2"/>
    <dbReference type="GeneID" id="8291109"/>
    <dbReference type="KEGG" id="lth:KLTH0C00528g"/>
    <dbReference type="eggNOG" id="KOG3079">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032354_0_0_1"/>
    <dbReference type="InParanoid" id="C5DDF2"/>
    <dbReference type="OMA" id="EQTMPVI"/>
    <dbReference type="OrthoDB" id="442176at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033862">
        <property type="term" value="F:UMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006221">
        <property type="term" value="P:pyrimidine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01428">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000315">
        <property type="entry name" value="Adenylate kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00235">
        <property type="entry name" value="Adenylate_kinase_Adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03172">
        <property type="entry name" value="Adenylate_kinase_UMP_CMP_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000850">
        <property type="entry name" value="Adenylat/UMP-CMP_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033690">
        <property type="entry name" value="Adenylat_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006266">
        <property type="entry name" value="UMP_CMP_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057781">
        <property type="entry name" value="YKL023C-A-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01359">
        <property type="entry name" value="UMP_CMP_kin_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23359">
        <property type="entry name" value="NUCLEOTIDE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00406">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23521">
        <property type="entry name" value="YKL023C-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00094">
        <property type="entry name" value="ADENYLTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00113">
        <property type="entry name" value="ADENYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 9 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6 9" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9 10" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="NMPbind" evidence="9">
        <location>
            <begin position="123"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="LID" evidence="9">
        <location>
            <begin position="218"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="103"/>
            <end position="108"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>a ribonucleoside 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="151"/>
            <end position="153"/>
        </location>
        <ligand>
            <name>a ribonucleoside 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="181"/>
            <end position="184"/>
        </location>
        <ligand>
            <name>a ribonucleoside 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>a ribonucleoside 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>a ribonucleoside 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>a ribonucleoside 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR21813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="282" mass="31637" checksum="90C8EBA263719174" modified="2009-07-28" version="1">MLRTAFRARIGPQTALRTVKTSQLLLPRAARFYSSNGGSQQPKSRGKLLVLMGALALGSTLFSINYQKNPPVAFVEEENDKKTQRPAFSEKEVSVIFVLGGPGAGKGTQCANLVRDFQFVHLSAGDLLRAEQNRSNSKYGELIKHYIKEGLIVPQEVTVALLKQAIQESYEKGQKRFLVDGFPRKMDQAVTFEEEIVPSKFTLFFDCPESVMLKRLLERGKTSGRSDDNVESIKKRFKTFIDTSMPVVKHFEEQGKVVTVSCDHDMNEVYNQVKAAVKDKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDJ1</accession>
    <name>C5DDJ1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0C01408p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0C01408g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552290.1">
        <property type="nucleotide sequence ID" value="XM_002552244.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDJ1"/>
    <dbReference type="GeneID" id="8291148"/>
    <dbReference type="KEGG" id="lth:KLTH0C01408g"/>
    <dbReference type="eggNOG" id="ENOG502RY5S">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_469339_0_0_1"/>
    <dbReference type="InParanoid" id="C5DDJ1"/>
    <dbReference type="OMA" id="TIENWIP"/>
    <dbReference type="OrthoDB" id="4036607at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR21852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="517" mass="57218" checksum="4F9F6B71308EFC70" modified="2009-07-28" version="1">MKMSNYENMNMLVKSEGPVRNGLLSLPIEVVEMIVQQLTASPKTTVVDYYHLLQALGETNGPVAALLRSKIAVRDLTRGRCDLAKVLGGSKGSIKGDDTIPSQTSFYVVAIDEDALELAPGATALPAPYGDIQLSLVFHSDRQVPLTEIHEKFRRRFPQDCYRVQHLIVTSSRCQTLEFRANDGNLLDTRLYSERDPMFAQVTYLNASRIVIAATALTEFISGVARGTKTSMPELCYGCNSHLANTLKIHSMLEPPLPMVTRVPVNFPLVESVAFSGSDGSPGSLNVIDLYAFCRIREQDAKLTLNSLLSVYSLRHWNLPSVKRFTGHRLKLSDGSSDVHQRSRSARETITRIRTIAQQESPDGLLHVNAQLVPDGVVHTTIENWIPMEAKFGGRAGRDCSPLICFRNSSLKSLRLGLAMKPEGTLVVEGLYLPSLEKLDVTSYSDDTPRNEVRLPSYSITFSAWNDLDKCRGLGFSSAEFNGHSIVQNLNLKQVFPRLDVANTFRQKFSSSSVLAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDJ2</accession>
    <name>C5DDJ2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0C01430p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0C01430g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552291.1">
        <property type="nucleotide sequence ID" value="XM_002552245.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDJ2"/>
    <dbReference type="FunCoup" id="C5DDJ2">
        <property type="interactions" value="37"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDJ2"/>
    <dbReference type="GeneID" id="8291149"/>
    <dbReference type="KEGG" id="lth:KLTH0C01430g"/>
    <dbReference type="eggNOG" id="ENOG502S8JP">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078821_0_0_1"/>
    <dbReference type="InParanoid" id="C5DDJ2"/>
    <dbReference type="OMA" id="NENSTIM"/>
    <dbReference type="OrthoDB" id="4035272at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056940">
        <property type="entry name" value="PEX18_PEX21_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25098">
        <property type="entry name" value="PEX18_PEX21_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="PEX18/PEX21 C-terminal" evidence="7">
        <location>
            <begin position="185"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="157"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="161"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF25098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR21853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="276" mass="30311" checksum="DAD764E23E710E48" modified="2009-07-28" version="1">MSLCQAGANPISRLAGKSEQLRGVGRLHDARPGVGGVRDGREAQFRGAGASAQPMAQPVPHPMAAAPAAADAWVNQFSNMHIKDPLSFSSEYQRLYSSYEQPQAVRPAQPQTLHAVQPQYQRQFYPSSYSQSLSQQFSQSFTADNSALFDSEFDQIEQEEAAQQQQQQQQQGPAAQDTATAVPALDDEQAKFQQAATEIYARLSPGPQTPPEMEAKLHNPKLQNSKFLGLMRRISDGVVTLQNDQNSQKYTQLYSPTTGEVVGNEYFPVVDAILEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDK4</accession>
    <name>C5DDK4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0C01716p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0C01716g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552303.1">
        <property type="nucleotide sequence ID" value="XM_002552257.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDK4"/>
    <dbReference type="GeneID" id="8291162"/>
    <dbReference type="KEGG" id="lth:KLTH0C01716g"/>
    <dbReference type="HOGENOM" id="CLU_744086_0_0_1"/>
    <dbReference type="InParanoid" id="C5DDK4"/>
    <dbReference type="OrthoDB" id="10348446at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR21865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="372" mass="42840" checksum="40964957C49A808B" modified="2009-07-28" version="1">MFFDPNEMNGIQNGRIEPLGLEAYSVEATTPVKPLDGLLFSSVENCCINYLEGHNQPLYQIFSPNDKTYEDEKARSLGNLSRKSSWESAEPKTCFLVSRSETFDHYSPYETTANGDLEIDGLNDRLLIRESNIRSVSKLDMTLVPLKIEFEDWNPFRYSKKVSKYCSDSSETRQFLKIRKVMQNNVIRLYFQLESSSGSQAFNEGEMRISLINRRVNPLTFDNSSLNKHNYDDSEDQLIDMAFLTVSEFISVIKFILKDLDKGSPELRFKLKRDFPASSSYDPYLPNRIRSNIREFLKGKTLRGFVPNDFAEKLLPSQIDFMIGLFAQISNYETEKPTRNNSQLSVVKLEDFEKAVKKCVSFYFFALPNGQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDQ7</accession>
    <name>C5DDQ7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0C02970p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0C02970g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552356.1">
        <property type="nucleotide sequence ID" value="XM_002552310.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDQ7"/>
    <dbReference type="FunCoup" id="C5DDQ7">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDQ7"/>
    <dbReference type="GeneID" id="8291218"/>
    <dbReference type="KEGG" id="lth:KLTH0C02970g"/>
    <dbReference type="eggNOG" id="ENOG502QVFG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036942_1_0_1"/>
    <dbReference type="InParanoid" id="C5DDQ7"/>
    <dbReference type="OMA" id="PYIPFRI"/>
    <dbReference type="OrthoDB" id="2128042at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015079">
        <property type="term" value="F:potassium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031606">
        <property type="entry name" value="Kch1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36424:SF1">
        <property type="entry name" value="LOW AFFINITY K(+) TRANSPORTER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36424">
        <property type="entry name" value="PHEROMONE-REGULATED MEMBRANE PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16944">
        <property type="entry name" value="KCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="34"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="81"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="212"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="425"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="491"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="442"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR21918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="516" mass="58260" checksum="50FD6D1470B80B93" modified="2009-07-28" version="1">MFRNDWKLSINSKTFDALDCSLFRNFKFMTVVNYAFFVLFLTAMKIALFISDVYTCIKLLAFNSWSNNVVPPYIPFRISKWLFSGCILASIVLIAWEIINGIRIYRTRNISLTYVNNFSRGAYSLSQYNKFCVYNRISPSGAYQKIAFFTFFELKDCLRLLVADTPRQVINGLTLWSVVVTVDQSSDLGRVESFSGLVAKIKTIAQTNHEEAVLLSFMLFSFVIWAFFITKFVAACLCSIYVYYRIFNERNASGLKEYVCVTVSEHIDYLVDKYQRRKQFNMVNQSHGLSSSSLLQTDDLEAGPWKSAAHTNVESLELEDRSESRVNSSTANLLAKEFAAPVAPRLNKWASVDSLPTIGTADPVQAASPIANMHQTAPQKRTAFDPIARSETFESSRPVKENPFAPRLINANTFNSVKTSNTVPSYYFDRDASPETPGAPEASRDEPDPAQRRQRPPPPAPGKVNTPEQAYFGEFAASSDSLAQRNASVLARRDRIEREDYEFYTQSRHASGGARP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDU4</accession>
    <name>C5DDU4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">homocysteine S-methyltransferase</fullName>
            <ecNumber evidence="10">2.1.1.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0C03850g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Homocysteine S-methyltransferase involved in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio. Also converts S-methylmethionine (SMM) to methionine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>S-methyl-L-methionine + L-homocysteine = 2 L-methionine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:26337"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
            <dbReference type="ChEBI" id="CHEBI:58252"/>
            <dbReference type="EC" id="2.1.1.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.1.1.10" evidence="10"/>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552393.1">
        <property type="nucleotide sequence ID" value="XM_002552347.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDU4"/>
    <dbReference type="FunCoup" id="C5DDU4">
        <property type="interactions" value="227"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDU4"/>
    <dbReference type="GeneID" id="8291257"/>
    <dbReference type="KEGG" id="lth:KLTH0C03850g"/>
    <dbReference type="eggNOG" id="KOG1579">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004914_3_2_1"/>
    <dbReference type="InParanoid" id="C5DDU4"/>
    <dbReference type="OMA" id="TECYEAQ"/>
    <dbReference type="OrthoDB" id="261426at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008898">
        <property type="term" value="F:S-adenosylmethionine-homocysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.330:FF:000005">
        <property type="entry name" value="AdoMet-homocysteine methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.330">
        <property type="entry name" value="Homocysteine-binding-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017226">
        <property type="entry name" value="BHMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003726">
        <property type="entry name" value="HCY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036589">
        <property type="entry name" value="HCY_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11103:SF10">
        <property type="entry name" value="HOMOCYSTEINE S-METHYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11103">
        <property type="entry name" value="SLR1189 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02574">
        <property type="entry name" value="S-methyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037505">
        <property type="entry name" value="Betaine_HMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82282">
        <property type="entry name" value="Homocysteine S-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50970">
        <property type="entry name" value="HCY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="3 13" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 13" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Hcy-binding" evidence="14">
        <location>
            <begin position="6"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="308"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00052655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00053380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR21955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="329" mass="36809" checksum="F0E1FBA18A4BDEF2" modified="2009-07-28" version="1">MPRPSVKEYLASNPEEVLVLDGGQGTELENRGIKVANPVWSTIPFISDSFWTANDSSSKDRQIVKEMYEDFLKAGARVLMTVTYQASFKSVSENTSITTLEEYDALLSRIVSFSRSCIGDDKWLVGCIGPWGAHNCSEFTGDYGSEPEKIDYLQYFKPQLDNFTVNDDLDLIGFETIPNIHELRAILSWDTTILPKPFYIGLSVHEHGVLRDGTTMHEVAELIKSLGDKINPNFVLLGINCVSYNHSPEILRSLHQEIPELPLIAYPNSGEVYDTVKKIWNPKGDHTLTWDQVVKSYIDSGARIIGGCCRTSPKDIAAINAAVNKYRPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DE22</accession>
    <name>C5DE22_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">alpha,alpha-trehalase</fullName>
            <ecNumber evidence="5">3.2.1.28</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0C05676g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>alpha,alpha-trehalose + H2O = alpha-D-glucose + beta-D-glucose</text>
            <dbReference type="Rhea" id="RHEA:32675"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15903"/>
            <dbReference type="ChEBI" id="CHEBI:16551"/>
            <dbReference type="ChEBI" id="CHEBI:17925"/>
            <dbReference type="EC" id="3.2.1.28"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyl hydrolase 65 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.28" evidence="5"/>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552471.1">
        <property type="nucleotide sequence ID" value="XM_002552425.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DE22"/>
    <dbReference type="FunCoup" id="C5DE22">
        <property type="interactions" value="79"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DE22"/>
    <dbReference type="GeneID" id="8291339"/>
    <dbReference type="KEGG" id="lth:KLTH0C05676g"/>
    <dbReference type="eggNOG" id="KOG4125">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006285_4_0_1"/>
    <dbReference type="InParanoid" id="C5DE22"/>
    <dbReference type="OMA" id="DIALAHW"/>
    <dbReference type="OrthoDB" id="200349at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004555">
        <property type="term" value="F:alpha,alpha-trehalase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015976">
        <property type="term" value="P:carbon utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005993">
        <property type="term" value="P:trehalose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000032">
        <property type="entry name" value="Vacuolar acid trehalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.40">
        <property type="entry name" value="Glycoside hydrolase, family 65, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037018">
        <property type="entry name" value="GH65_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005195">
        <property type="entry name" value="Glyco_hydro_65_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005196">
        <property type="entry name" value="Glyco_hydro_65_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11051">
        <property type="entry name" value="GLYCOSYL HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11051:SF8">
        <property type="entry name" value="PROTEIN-GLUCOSYLGALACTOSYLHYDROXYLYSINE GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03632">
        <property type="entry name" value="Glyco_hydro_65m"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03636">
        <property type="entry name" value="Glyco_hydro_65N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0574">Periplasm</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 65 N-terminal" evidence="12">
        <location>
            <begin position="104"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 65 central catalytic" evidence="11">
        <location>
            <begin position="434"/>
            <end position="654"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF03636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR22033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1152" mass="129460" checksum="7163E1BC46DB2AEC" modified="2009-07-28" version="1">MSVRHKTRKHTATSLIARSLPGLRAIFVILATCVAAAGWRWLLTGELPRKPHLPCKVFRTHRHHDTRIEWRRRSSESLYELLTDSQDTYYDEDNWTVGTVFFSENTYSRQPYVANGFLGSRIPNVGFGYAPDTYNIWTPDPSIPGALNNGWPLRNQRFAGAFVSDFYSLQPHLNSTNFPELDADGYSTILSAIPQWTDMKISVKNDTVRFTAQDVLPQHVSNYHQNLSLLTGTVTTELDWMGLLHIKSTVLAHRILHPLGVLKLELSLIPDSGLKTLDLQVSDIFNHSTSRRTFLRDLGYDSRGIYMEVEPDNVPYSSAALYSSLRFDGPAEADGCIFQLSDMNTTVSRKNFLTLSCEKPSITIQKYVAVVSTEYDRYNKTASNMEAAKDIVHSQCGQYDLITQAHHNEWVSLYDNASIEIPSDSLLELSAKSSLFHLLANTRRFNISSSRGLPLPSSGLSSDSYGGMIFWDADIWILPALLPFFPDIARHMVDYRKNTHEQARLNAQQYGYPGALYPWTSGRYANCTSTGPCVNYEYHINIDIAMASFLVYLNGAPGVDDEYLRETTWPLVRDAAQFFTAYVKFNSSLDAYETHNMTDPDEYANFINNGAFTNAGIKTLLKWATDVGNHLQEEIDPRWVEVSDKMFIPIAESNITLEYSGMNASVEIKQADVMLMTYPLGYITDETILDNAIRNLYYYSERQSPSGPAMTYPVFVAAAAGLLNHGCSSQSYLYKSVLPYLRAPFAQFSEQSDDNFLTNGFTQPAFPFLTGNGGYLQSLLFGLTGLRYSYEVDKQTKKITRLLKFNPIKLPLLQGGIAINNFKYMGQVLDIIIGDEKATIVHKIGDEPINLKLIDRSFIHDRDVTGPHMTHVHEGEEVPVQWITLQPGQNLTVDLFRPLLNIDGNLIEGKQVSNFTAGVPGDVIYSVIDGNNFTHWQPLDKNVCAKILIDLGPGNEQNLTGGMILWGRRPARNISVAMVPYKGDIEQALQKASSVMESSGPDKRVEILGYTAPASELASRLSETEDRFFEEDYRLLLSSQRLDIEQLSKLSPESSLLKSKFVTILHNLQISPSEPYAEENQRGSKIVIPRSNSTHFTFDYSNYTSDKAFEHPVSKESGASWPPPRFVLVCIQGTYDDDDDPKGATIKEIALM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEC5</accession>
    <name>C5DEC5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0C08074p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0C08074g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. CAR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552574.1">
        <property type="nucleotide sequence ID" value="XM_002552528.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEC5"/>
    <dbReference type="FunCoup" id="C5DEC5">
        <property type="interactions" value="33"/>
    </dbReference>
    <dbReference type="GeneID" id="8291447"/>
    <dbReference type="KEGG" id="lth:KLTH0C08074g"/>
    <dbReference type="eggNOG" id="KOG0255">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008455_8_5_1"/>
    <dbReference type="InParanoid" id="C5DEC5"/>
    <dbReference type="OMA" id="KNDIGHE"/>
    <dbReference type="OrthoDB" id="3936150at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015203">
        <property type="term" value="F:polyamine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010509">
        <property type="term" value="P:intracellular polyamine homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17323">
        <property type="entry name" value="MFS_Tpo1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1720.10:FF:000009">
        <property type="entry name" value="MFS multidrug transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1720.10">
        <property type="entry name" value="Multidrug resistance protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF5">
        <property type="entry name" value="QUINIDINE RESISTANCE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="160"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="185"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="222"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="250"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="423"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="465"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="508"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="540"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="569"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="602"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="94"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="295"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="295"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR22136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="642" mass="71294" checksum="4830B013E272DD11" modified="2009-07-28" version="1">MMSGVQRAEQDADDLSLHTNSSLETSVGDEIGRPQTLHHNELSTSGTHLSKKSTLRDKSLMVPRKQRRGILANLSVVPEYKDARNYPGKLKAGIIFLIAYAAMMGPMGTSIIYPSINPISEDYKTTTMMVNVSVGIYLLSLGVLPLWWSSLSELRGRRTVYVLSFSLLCAFCVGSALAPNIQAFIVFRMLCGACSASVQSVGAGTVADLFKPEDRGKMLGYYYLGALMAPLLAPIIGSLLVSRWSWRSTQWFMVILAGVNVLLLTLLLPETLRSQDSKSAIAAILAERRNGAKAAAGATTDSASDASNAIKDKDEPEASNYDAEVQRVYSRASEAAQYPRDEEEHFGDTMVAQITRVQTRDPKLEMRMREGELKRWKTNLENELDRMETEKTSDKAEVLDLPKRSWSTTLYIYLIKPLKSLHFLRYPPVTLAITFSAISFAILYFVNMTLEYSYSRSPYNFKPLYVGLLYIPNSVTYVIASIYGGKWVDNLLRQYKKKHGTLAPEARISWNVVTAVVTFPISLLIFGWCLDKKQHWVTPLVGTALFGYASMMTIGATVAYLVDSLPGRGATGVALNNLIRQAFAATAVFVTEPMLEGMGPGWAFTMLAFIVIGASSVLVVLKMKGDYWRENYDLQKLYDKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEM5</accession>
    <name>C5DEM5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0C10450p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0C10450g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552674.1">
        <property type="nucleotide sequence ID" value="XM_002552628.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEM5"/>
    <dbReference type="STRING" id="559295.C5DEM5"/>
    <dbReference type="GeneID" id="8291554"/>
    <dbReference type="KEGG" id="lth:KLTH0C10450g"/>
    <dbReference type="eggNOG" id="KOG2743">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017452_2_1_1"/>
    <dbReference type="InParanoid" id="C5DEM5"/>
    <dbReference type="OMA" id="WSQAGPN"/>
    <dbReference type="OrthoDB" id="272672at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="CDD" id="cd03112">
        <property type="entry name" value="CobW-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011629">
        <property type="entry name" value="CobW-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003495">
        <property type="entry name" value="CobW/HypB/UreG_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051927">
        <property type="entry name" value="Zn_Chap_cDPG_Synth"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43603">
        <property type="entry name" value="COBW DOMAIN-CONTAINING PROTEIN DDB_G0274527"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43603:SF1">
        <property type="entry name" value="ZINC-REGULATED GTPASE METALLOPROTEIN ACTIVATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02492">
        <property type="entry name" value="cobW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07683">
        <property type="entry name" value="CobW_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00833">
        <property type="entry name" value="CobW_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90002">
        <property type="entry name" value="Hypothetical protein YjiA, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobW C-terminal" evidence="2">
        <location>
            <begin position="299"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="332"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="523"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="334"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="371"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="525"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR22236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="566" mass="63594" checksum="E50439D9EFB6D738" modified="2009-07-28" version="1">MTSDKKVSKAIPITLLSGFLGSGKTTLLEKILTTNHGFKIAVIINDMSELNIDSSLVQAHNVCQKEEKLIELQNGCICCTLRGDLLEELVGIGERGEFDYIVIESTGIAEPMQVAETFSVEFSDILLETPGGIPENEEKLLRKVSDMGGLQAIAKLDTCVTVIDALNFLSNIQTTEFLADRWGENGQGEAERTITDLMVDQIEFSDVIIVNKKSMVSKKKLKEIKKMVKILNPVAKVIATDYCNVPLSSILDTKLHDFEKASLSAGWLQSINEMTVRDGFGNKGSSVLTPKPETEEYGVNNFVYRRRRPFHPERLYDLIRDKFFVIEQAGAAGMDEEEEDDEEESEEQSSESEADSEMEAEVDEGARDGEEKEEEEGEEYTEEEINKNRANSPFGKLLRSKGFFWLATRFVVRGEWSSAGPMLTLNGGLPWFAVSGLEDVPEEALELVKKDYEGEFGDRRNELVFIGLDINEKLLSEALDSCLLTDKEYKRFKNITSKHNALVADEKLSKVWDDGFEDWIIMGAGDEEEEDREQEEEQEEEEEEEGKERSQKSVPMEISTSKGISV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEP7</accession>
    <name>C5DEP7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Palmitoyltransferase PFA4</fullName>
            <ecNumber evidence="11">2.3.1.225</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Protein S-acyltransferase</fullName>
            <shortName evidence="11">PAT</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Protein fatty acyltransferase 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">PFA4</name>
        <name evidence="14" type="ordered locus">KLTH0C10978g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Mediates the reversible addition of palmitate to target proteins, thereby regulating their membrane association and biological function.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Endoplasmic reticulum membrane</location>
            <topology evidence="11">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11 12">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the DHHC palmitoyltransferase family. PFA4 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="11"/>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552696.1">
        <property type="nucleotide sequence ID" value="XM_002552650.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEP7"/>
    <dbReference type="FunCoup" id="C5DEP7">
        <property type="interactions" value="41"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEP7"/>
    <dbReference type="GeneID" id="8291578"/>
    <dbReference type="KEGG" id="lth:KLTH0C10978g"/>
    <dbReference type="eggNOG" id="KOG1314">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027721_8_0_1"/>
    <dbReference type="InParanoid" id="C5DEP7"/>
    <dbReference type="OMA" id="TMNCVGY"/>
    <dbReference type="OrthoDB" id="331948at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03199">
        <property type="entry name" value="DHHC_PAT_PFA4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033682">
        <property type="entry name" value="PFA4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12246">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11 12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6 11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0564">Palmitate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11 12">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11 12">
        <location>
            <begin position="41"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11 12">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11 12">
        <location>
            <begin position="163"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="13">
        <location>
            <begin position="77"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="active site" description="S-palmitoyl cysteine intermediate" evidence="11">
        <location>
            <position position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR22258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="377" mass="43797" checksum="68E95C9601C36E8D" modified="2009-07-28" version="1">MPVELKWPWLGIAVPSFLIAYIGYSSHYFILQNFASTYQQLWFEFSLTLIWVSYAFAIFKNPGRPPPNFEPPIHQWKNYCKKCDNYKPERTHHCKTCKQCVLSMDHHCPWTMNCVGHNNFPHFVRFLTWVVLTTSYLGLLLTQRAFDHWASRNTRKFVHTSELCFLFVNAPLNAFILLTVSLLVFRCVKNQFLNGMTQIESWEKDRIENLFFNERLVPQLIDNLKELFPEDTFDEGAAQKLLSSKQLELEDIINFPYDVDILENMKSSMGPIYSWCLPCGRPRDEGTRFVTNDLSDYDIKASIEDKLLSLPWPPDGGRQKSQRSADIASTLETFSSGGEATLRNRSFDSRISVPRTEWHNDWGENLSDFGVDVDAET</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEQ2</accession>
    <name>C5DEQ2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0C11154p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0C11154g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="2">Binds 1 Fe cation per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the pirin family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552701.1">
        <property type="nucleotide sequence ID" value="XM_002552655.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEQ2"/>
    <dbReference type="GeneID" id="8291586"/>
    <dbReference type="KEGG" id="lth:KLTH0C11154g"/>
    <dbReference type="eggNOG" id="ENOG502QQ5A">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045717_0_2_1"/>
    <dbReference type="InParanoid" id="C5DEQ2"/>
    <dbReference type="OMA" id="TPWHPHR"/>
    <dbReference type="OrthoDB" id="198735at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02247">
        <property type="entry name" value="cupin_pirin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02909">
        <property type="entry name" value="cupin_pirin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012093">
        <property type="entry name" value="Pirin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008778">
        <property type="entry name" value="Pirin_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003829">
        <property type="entry name" value="Pirin_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13903:SF8">
        <property type="entry name" value="PIRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13903">
        <property type="entry name" value="PIRIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02678">
        <property type="entry name" value="Pirin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05726">
        <property type="entry name" value="Pirin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006232">
        <property type="entry name" value="Pirin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pirin N-terminal" evidence="4">
        <location>
            <begin position="22"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Pirin C-terminal" evidence="5">
        <location>
            <begin position="179"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR006232-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR22263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="305" mass="33730" checksum="AD99B9687A586861" modified="2009-07-28" version="1">MNKPRSILKYFTALEQSEGVGAKVRRSIGSQELRNFTPFLMLDHFNVSPPAGFPDHPHHGQETITYVLGGMIAHEDFTGSRGVLRPGDLQFMTAGKGIIHSEIPVELESGAPSVGLQLWVDLPMHLKDCEPRYRDLLNNEIPSVQPKAGLEVRVISGESYGVGSMSELAYTPVHFYHYITRKPGTTFEQHFPSNFNVFMYVLKGSVIVNGKTFAQYSSVFFNTDGSRVEGSGSSEESEFILIGGEVLDQPILQYGPFVETSKQNMYEVIGNYQSGTNGFERAQGWSSSIARGVVFSDVAKIESCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>C5DF29</accession>
    <name>C5DF29_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0D11770p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0D11770g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553222.1">
        <property type="nucleotide sequence ID" value="XM_002553176.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DF29"/>
    <dbReference type="FunCoup" id="C5DF29">
        <property type="interactions" value="508"/>
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    <dbReference type="STRING" id="559295.C5DF29"/>
    <dbReference type="GeneID" id="8295462"/>
    <dbReference type="KEGG" id="lth:KLTH0D11770g"/>
    <dbReference type="eggNOG" id="KOG0721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014210_0_0_1"/>
    <dbReference type="InParanoid" id="C5DF29"/>
    <dbReference type="OMA" id="ETWPFFL"/>
    <dbReference type="OrthoDB" id="1734229at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
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        <property type="term" value="C:Sec62/Sec63 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:protein transmembrane transporter activity"/>
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    </dbReference>
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        <property type="term" value="F:RNA binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:post-translational protein targeting to endoplasmic reticulum membrane"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SEC63 isoform 5"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="SEC63 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TRANSLOCATION PROTEIN SEC63 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158702">
        <property type="entry name" value="Sec63 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="14"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="212"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="3">
        <location>
            <begin position="115"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="603"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="612"/>
            <end position="652"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR22784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="652" mass="73682" checksum="78809085F4A628CF" modified="2009-07-28" version="1">MTQSYDYDEGSETWPFFVLAVLCVVLVPLTLMQAYEIIAAKDEDSSSQSSPLEKLSEKYTGDQVKRFRAKFAPKKSKLLARRNLVIIAGWCMVAFLINKIGSNGAIKDAATLLFDPYALLDISASSTDREIRSAYRKLSVKFHPDKLSKDLSADERTVLEEQYVMITKAYKALTDEITRENYLRYGHPDGPQATSHGIALPKFLVDSSASTLMVLGYIALLGFILPFFVSRWWSKTQSFTKKGIHARSASYFADRLFNFKPSQVVTTGLILNWLSHAAEFKLLYPDLTQETFESLLYDHINRRHSGELETAKLRIVAKTHALLFGLLDIATTFRNLEISTVAIDTFKSIVQATPLTSHSQILQLPNVDAKRFEEGSVDDIRTLGKLFTFEDEKIGKILGIEDETKLRETLSVAANIPHLKLLRAEFTVPGESVVTPLSTPHISVKVLVRSAKHKFFTPNKIPDELYKEEQDFDSLRDPFASLSNQPALPRTFAPHFPAKRSGCWCALVVLQKDGKVIQTPMLVDRLSFANLNKDFDKRTVKDLEKEFNPEEWEIGTIKIPFSQPAPDQKGDYYFRVIVKSTEYFGADLDFTLPMMVRELSEVEGAEKIADDSASDEDDSDLEDDEEDSNDELEDDEDSDYTDIDTDTEVEEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>C5DF72</accession>
    <name>C5DF72_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0D12738p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0D12738g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553265.1">
        <property type="nucleotide sequence ID" value="XM_002553219.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DF72"/>
    <dbReference type="FunCoup" id="C5DF72">
        <property type="interactions" value="299"/>
    </dbReference>
    <dbReference type="GeneID" id="8295505"/>
    <dbReference type="KEGG" id="lth:KLTH0D12738g"/>
    <dbReference type="eggNOG" id="KOG2551">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051938_2_2_1"/>
    <dbReference type="InParanoid" id="C5DF72"/>
    <dbReference type="OMA" id="EEPRGWW"/>
    <dbReference type="OrthoDB" id="2094269at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005645">
        <property type="entry name" value="FSH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050593">
        <property type="entry name" value="LovG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48070">
        <property type="entry name" value="ESTERASE OVCA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48070:SF6">
        <property type="entry name" value="ESTERASE OVCA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03959">
        <property type="entry name" value="FSH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine hydrolase" evidence="2">
        <location>
            <begin position="3"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR22827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="255" mass="28303" checksum="DA2BC0A0F1650AC1" modified="2009-07-28" version="1">MAIKVLMLHGYAQSGPIFYAKSSGLRKSLAKLGYELYYPSAPSRIPSVDISSVDDMASVFNTNAGTENIFGWWLKDPHDQYKVPQSTTDFLHDYIVENGPFDGIAGFSQGAGYAGYLCTNVRQLLGLTAEQQPDFKFFISFSGFRMHPEWFQEQYNRHPITVPSLHVMGELDTVVESSRVMRLYNSCSEGTRTLLKHPGGHFVPNGKGFVAKVANWLQMAGEAPKQKCDNEAQQNPEQPDLGDDLLDAIDGLGKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>C5DF85</accession>
    <name>C5DF85_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0D13068p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0D13068g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553278.1">
        <property type="nucleotide sequence ID" value="XM_002553232.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DF85"/>
    <dbReference type="GeneID" id="8295520"/>
    <dbReference type="KEGG" id="lth:KLTH0D13068g"/>
    <dbReference type="HOGENOM" id="CLU_1454669_0_0_1"/>
    <dbReference type="InParanoid" id="C5DF85"/>
    <dbReference type="OMA" id="LEMPWIG"/>
    <dbReference type="OrthoDB" id="1259151at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR22840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="183" mass="20358" checksum="D69D63EDA701AB5E" modified="2009-07-28" version="1">MSINSLTMQDSGVLPFLPLDGESTPYGAVLTSTAWYPLMGPGLKPNKKYEGAIRMNFKLFVTSHRLVFVNCSQGAEEEVKNIVVLYRQLVLPAGVSQPLTLEMPWIGSNYLRFAFRVLPEQTGQNGQRLNSIYTWLCDVAMEKGAPSLKDAFQLHDAIRQALADRTSAEGEAHQEEPLPLYTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>C5DF95</accession>
    <name>C5DF95_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0D13310p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0D13310g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553288.1">
        <property type="nucleotide sequence ID" value="XM_002553242.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DF95"/>
    <dbReference type="FunCoup" id="C5DF95">
        <property type="interactions" value="325"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DF95"/>
    <dbReference type="GeneID" id="8295530"/>
    <dbReference type="KEGG" id="lth:KLTH0D13310g"/>
    <dbReference type="eggNOG" id="KOG0873">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047036_5_0_1"/>
    <dbReference type="InParanoid" id="C5DF95"/>
    <dbReference type="OMA" id="EIMFYYA"/>
    <dbReference type="OrthoDB" id="1658724at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050307">
        <property type="entry name" value="Sterol_Desaturase_Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11863">
        <property type="entry name" value="STEROL DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="128"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="208"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid hydroxylase" evidence="7">
        <location>
            <begin position="147"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="287"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR22850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="309" mass="36442" checksum="86DAB029F7C7FDC9" modified="2009-07-28" version="1">MSAVFNNATLATTLAQGKSYMQTLHNVRAFQPQLNTMEQYWAAWYSYMNNDVLATGLMFFLLHEFMYFARCLPWAVIDQIPYFRRWKLQPTKIPSAKEQWYCLKSVLLSHFLVEAIPIWTFHPMCQKLGISVSVPFPSIKTMVSQIVLFFVLEDMWHYWAHRLFHYGVFYKYIHKQHHRYAAPFGLAAEYAHPVETLSLGFGTVGMPILYVMYTGNLHLFTLCLWITLRLFQAVDSHSGYDFPWSLNKFLPLWAGAEHHDLHHHYFIGNYASSFRWWDYTLDTEAGPEAKAEREERMKRKAEQRAKKAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFC6</accession>
    <name>C5DFC6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0D14036p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0D14036g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CENP-I/CTF3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553319.1">
        <property type="nucleotide sequence ID" value="XM_002553273.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFC6"/>
    <dbReference type="FunCoup" id="C5DFC6">
        <property type="interactions" value="94"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFC6"/>
    <dbReference type="GeneID" id="8295563"/>
    <dbReference type="KEGG" id="lth:KLTH0D14036g"/>
    <dbReference type="eggNOG" id="ENOG502QS8Q">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022668_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFC6"/>
    <dbReference type="OMA" id="NYLWRNK"/>
    <dbReference type="OrthoDB" id="6347512at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000939">
        <property type="term" value="C:inner kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034080">
        <property type="term" value="P:CENP-A containing chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000070">
        <property type="term" value="P:mitotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22647">
        <property type="entry name" value="CTF3_NTD_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012485">
        <property type="entry name" value="CENP-I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48208">
        <property type="entry name" value="CENTROMERE PROTEIN I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48208:SF2">
        <property type="entry name" value="CENTROMERE PROTEIN I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07778">
        <property type="entry name" value="CENP-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0137">Centromere</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR22881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="693" mass="80373" checksum="6E8979278629EBDF" modified="2009-07-28" version="1">MNTVDESFKELISYSKHSPLEVSACLSRIHSTVCTEGVSASVLDDVIVFLCESPLISVSTKVYLIHEALLPNGPVQSSTVFTILSHLGVRSFTNPHKRETHPDIQIELCKWLVQVYVLVDDVTVFSKTYSIWFQLWQFDYLQKWVTYLLFWTTTAEAVRPWRIQWLLETSLNTGYSNSKALATLLLEKFNIARPSQAIVDAISSVHCNRRRLKSLEFDSYDESFLSNWSKVLDHSRCISMPAFFDLISDHRSQIHLVATESKTERLQLSQAVPLNGVETVEKLVSSYYNITPPKNVEEVLPNGDRTVMIYLALLERRNPFWDRCLKWCELRLKSEVFASRNDPERTLETMKIVMLTLALYQTDPSISDEVRAENRITNLLRQMATQSRFSYIALLLAPPMTYVETTMARELTERLKLGSKLSAFYERCRHMLYFLWASIGTLVDKSLLRKLSSDFEETLRLINEDLSSCSSNRHVNMTLRLLIKVFSFILLRYKHMPDWDITSFYKLLEVLIITNDPLALCSIVEFFSKAREYVQEHSKDRSLVEIHNNAVLDVTNYLWRNKFKNNASFIGVPSDFIQKIIESLYSEDSEMSLKSWLTITSIPATSYCSLQILRSFERATASKISFTRLLTDKSYEAFKKQVSSEDWLDTIPTYYDLKLTILARMRFDRTYQNIPEFLFTFLRSLTGTQKSIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFH2</accession>
    <name>C5DFH2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Fatty acid synthase subunit beta</fullName>
            <ecNumber evidence="21">2.3.1.86</ecNumber>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="21">3-hydroxyacyl-[acyl-carrier-protein] dehydratase</fullName>
                <ecNumber evidence="21">4.2.1.59</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="21">Enoyl-[acyl-carrier-protein] reductase [NADH]</fullName>
                <ecNumber evidence="21">1.3.1.9</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="21">[Acyl-carrier-protein] acetyltransferase</fullName>
                <ecNumber evidence="21">2.3.1.38</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="21">[Acyl-carrier-protein] malonyltransferase</fullName>
                <ecNumber evidence="21">2.3.1.39</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="21">S-acyl fatty acid synthase thioesterase</fullName>
                <ecNumber evidence="21">3.1.2.14</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="24" type="ordered locus">KLTH0D15070g</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="20 21">Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. The beta subunit contains domains for: [acyl-carrier-protein] acetyltransferase and malonyltransferase, S-acyl fatty acid synthase thioesterase, enoyl-[acyl-carrier-protein] reductase, and 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17 21">
            <text>(9Z)-octadecenoyl-[ACP] + H2O = (9Z)-octadecenoate + holo-[ACP] + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15057"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9924"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78783"/>
            <dbReference type="EC" id="3.1.2.14"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 21">
            <text>a (3R)-hydroxyacyl-[ACP] = a (2E)-enoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:13097"/>
            <dbReference type="Rhea" id="RHEA-COMP:9925"/>
            <dbReference type="Rhea" id="RHEA-COMP:9945"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78784"/>
            <dbReference type="ChEBI" id="CHEBI:78827"/>
            <dbReference type="EC" id="4.2.1.59"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18 21">
            <text>a 2,3-saturated acyl-[ACP] + NAD(+) = a (2E)-enoyl-[ACP] + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10240"/>
            <dbReference type="Rhea" id="RHEA-COMP:9925"/>
            <dbReference type="Rhea" id="RHEA-COMP:9926"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:78784"/>
            <dbReference type="ChEBI" id="CHEBI:78785"/>
            <dbReference type="EC" id="1.3.1.9"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 21">
            <text>acetyl-CoA + n malonyl-CoA + 2n NADPH + 4n H(+) = a long-chain-acyl-CoA + n CoA + n CO2 + 2n NADP(+).</text>
            <dbReference type="EC" id="2.3.1.86"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19 21">
            <text>holo-[ACP] + acetyl-CoA = acetyl-[ACP] + CoA</text>
            <dbReference type="Rhea" id="RHEA:41788"/>
            <dbReference type="Rhea" id="RHEA-COMP:9621"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78446"/>
            <dbReference type="EC" id="2.3.1.38"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 21">
            <text>holo-[ACP] + malonyl-CoA = malonyl-[ACP] + CoA</text>
            <dbReference type="Rhea" id="RHEA:41792"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="EC" id="2.3.1.39"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="14 21">[Alpha(6)beta(6)] hexamers of two multifunctional subunits (alpha and beta).</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 21">Belongs to the fungal fatty acid synthetase subunit beta family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.86" evidence="21"/>
    <dbReference type="EC" id="4.2.1.59" evidence="21"/>
    <dbReference type="EC" id="1.3.1.9" evidence="21"/>
    <dbReference type="EC" id="2.3.1.38" evidence="21"/>
    <dbReference type="EC" id="2.3.1.39" evidence="21"/>
    <dbReference type="EC" id="3.1.2.14" evidence="21"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553365.1">
        <property type="nucleotide sequence ID" value="XM_002553319.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DFH2">
        <property type="interactions" value="746"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFH2"/>
    <dbReference type="GeneID" id="8295609"/>
    <dbReference type="KEGG" id="lth:KLTH0D15070g"/>
    <dbReference type="eggNOG" id="ENOG502QQJX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000114_5_0_1"/>
    <dbReference type="InParanoid" id="C5DFH2"/>
    <dbReference type="OMA" id="HFMDNYG"/>
    <dbReference type="OrthoDB" id="5417908at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005835">
        <property type="term" value="C:fatty acid synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019171">
        <property type="term" value="F:(3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004313">
        <property type="term" value="F:[acyl-carrier-protein] S-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004314">
        <property type="term" value="F:[acyl-carrier-protein] S-malonyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004318">
        <property type="term" value="F:enoyl-[acyl-carrier-protein] reductase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004312">
        <property type="term" value="F:fatty acid synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016297">
        <property type="term" value="F:fatty acyl-[ACP] hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004321">
        <property type="term" value="F:fatty-acyl-CoA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03447">
        <property type="entry name" value="FAS_MaoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.120:FF:000001">
        <property type="entry name" value="Fatty acid synthase beta subunit dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.930.70:FF:000001">
        <property type="entry name" value="Fatty acid synthase beta subunit dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.10:FF:000017">
        <property type="entry name" value="Fatty acid synthase beta subunit dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000078">
        <property type="entry name" value="Fatty acid synthase beta subunit dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1120.100:FF:000001">
        <property type="entry name" value="Fatty acid synthase beta subunit dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.366.10:FF:000006">
        <property type="entry name" value="Fatty acid synthase beta subunit dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.366.10:FF:000007">
        <property type="entry name" value="Fatty acid synthase beta subunit dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.10:FF:000015">
        <property type="entry name" value="Fatty acid synthase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000169">
        <property type="entry name" value="Fatty acid synthase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2430:FF:000001">
        <property type="entry name" value="Fatty acid synthase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.366.10:FF:000003">
        <property type="entry name" value="Fatty acid synthase subunit beta dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1400">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1850">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.60.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.240.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.366.10">
        <property type="entry name" value="Malonyl-Coenzyme A Acyl Carrier Protein, domain 2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001227">
        <property type="entry name" value="Ac_transferase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014043">
        <property type="entry name" value="Acyl_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039569">
        <property type="entry name" value="FAS1-like_DH_region"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016452">
        <property type="entry name" value="Fas1/AflB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013565">
        <property type="entry name" value="Fas1/AflB-like_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041099">
        <property type="entry name" value="FAS1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040883">
        <property type="entry name" value="FAS_meander"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003965">
        <property type="entry name" value="Fatty_acid_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050830">
        <property type="entry name" value="Fungal_FAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002539">
        <property type="entry name" value="MaoC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032088">
        <property type="entry name" value="SAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10982:SF21">
        <property type="entry name" value="FATTY ACID SYNTHASE SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10982">
        <property type="entry name" value="MALONYL COA-ACYL CARRIER PROTEIN TRANSACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00698">
        <property type="entry name" value="Acyl_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08354">
        <property type="entry name" value="Fas1-AflB-like_hel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13452">
        <property type="entry name" value="FAS1_DH_region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22235">
        <property type="entry name" value="FAS1_thioest_ins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17951">
        <property type="entry name" value="FAS_meander"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17828">
        <property type="entry name" value="FAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01575">
        <property type="entry name" value="MaoC_dehydratas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16073">
        <property type="entry name" value="SAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005562">
        <property type="entry name" value="FAS_yeast_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01483">
        <property type="entry name" value="FASYNTHASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00827">
        <property type="entry name" value="PKS_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 21" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="6 21" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5 21" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 21" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="10 21" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 21" id="KW-0456">Lyase</keyword>
    <keyword evidence="13 21" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="9 21" id="KW-0520">NAD</keyword>
    <keyword evidence="7 21" id="KW-0521">NADP</keyword>
    <keyword evidence="8 21" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 21" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Malonyl-CoA:ACP transacylase (MAT)" evidence="23">
        <location>
            <begin position="1665"/>
            <end position="2018"/>
        </location>
    </feature>
    <feature type="active site" description="For acetyltransferase activity" evidence="22">
        <location>
            <position position="277"/>
        </location>
    </feature>
    <feature type="active site" description="For malonyltransferase activity" evidence="22">
        <location>
            <position position="1808"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00033756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00058855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRNR" id="PIRNR005562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR005562-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="SMART" id="SM00827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="CAR22927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="2050" mass="228422" checksum="06EBA56579F1C082" modified="2009-07-28" version="1">MSSSNSTRALTLSHGSIEHVLPVPTAIYFQGSQLQDQFSKQLPAASEGFVADNEPSSPAELLAKFLGYVASLVDPSTQGQFDDVLKISLAEFETSYLQGNDVHTLAAKLLADEDVYPTTLDKVKELIKNYFNARVAAQKPFTRSDSALIKSAAKKDSQLVAIFGGQGNTDDYFEELRELYQTYHSIISDVVETAASKLAELVRSTDDTEKVYTQGFDVLQWLQHPEKTPDNTYLLSIPISCPLIGVIQLVHYAVTARVLGLTPGQLREHLVGATGHSQGLATAVAIAEADSWESFEESLNKTITLLFFIGVRCYQAYPNTSLPPSMLEDSAENGEGTPSPMLSISNLTKDQVQKYVDQTNAHLPTEKHIVISLVNGARNLVVTGPPQSLYGLNLTLRKAKAPAGLDQARIPHSERKLKFFNRFLPIASPFHSHLLEPANEFIKQDLKDAGLEFKASDLKIPVYDTFDGKDLRSYSGSIAERITLCITKLPVNWELTTRFHATHILDFGPGGSSGLGVLTHRNKDGTGVRVIVAGALEQNLDDEFGFKQEIFDISSEGLKYSPNWLKEYHPKLVKTKAGKVYVDTKFSKLLGRAPLLVPGMTPCTVSPDFVASTINAGYTIELAGGGYFTPQHMIGAIDSVVSQIKKGYGLAINLIYVNPRMLQWGIPMIKELREKGYPIHSLTIGAGVPSLEVASEYIETLGLTYLGLKPGSIDAISQVITIAKAHPNFPIVLQWTGGRGGGHHSFEDFHALMLQMYSKIRRHSNIILVAGSGFGSAEDTYPYLTGEWSKKFNYPPMPFDGFLFGSRVMIAKEAKTSPDAKKAIAACTGVPDEQWEQTYKKPTGGIVTVRSEMGEPIHKLATRGVMLWKELDETIFNLPKNKLQPALDAKKDYIIGKLNADFQKPWFATVNGQVRDLTDMNYQEVAERMVELMYLKSQNKWIDVSWRNFTGDFLRRVEERFTKKKTASIIQSYTMLEEPEKVLTAIFDAYPAAKTQFLNAQDVDYFLTLCQRPTQKPVPFVPVLDHRFEFFFKKDSLWQSEYLEAVVDQDVGRTCILHGPVAAQFTNKVDEPIQSIMDGVHNGHIQKLLKDYYNSDESKIPVVEYFGGEDPETVKFDSAETDGKIIYRASASTDKVEWFKLLAGNTRNWRHAFFSTPRVVQDSLYAENPVQKVFKPTANSVVQISNPEDASKTTITLLEPVQGALKPTVSLRLVKENLMELQLIENRTIDGTPVSLPLLYKYQPDDGFAPIAEVMDGRNDRIKELYWKLWIDEPFNLDFDPKNPIQGGEVTITAKDIAEFTHAIGNKCEDFVSRPGRKLLAPMDFAIVVGWRAIIKAIFPKTVDGDLLKLVHMSNGYRMIPGAEPLQEGDVISSNAVIKSVVNQPSGKIVEVIGTLSRKGSPVMEVTSSFFYRGKYEDFENTFQKVVEPVYQVQIKSAKDVAVLRSKEWFQLDDEDVDLLGKTLTFETETAVTFKNETVFNSVKCEGKIKMELPTKENVEIGVVDYEAGESHGNPVIDYLNRHGSTLEQKVNLENAIPIAVKDSQAPGTNEPYARVSGDLNPIHVSRHFADYANLPGTITHGMYSSAAVRALVETWAADNVSSRVRAYSCQFVGMVLPNTPLKTSIQHVGMINGRKLIKFETKNDLEEVVMVGEAEVEQPVSTFVFTGQGSQEQGMGMDLYEKSEVAQVVWDRADAHFKKTYGFSILEIVRKNPTEMTIFFGGEKGRKIKSNYTQMIFETIVDGELKTERIFKDITEETTSYTFKSPTGLLSATQFTQPALTLMEKASFEDLKAKGLIPADAAFAGHSLGEYAALASLADVMSIESLVEVVFYRGMTMQVAVPRDDSGRSNYGMVAVNPGRVSPTFNQDALKFVVKCVDKRTGWLVEIVNYNVENQQYVAAGDLRALDTLTNVLNFVKVQKIDIVKLQASMSLEDVESHLNEIIDEVSKKSTAKPQPIELERGFACIPLRGISVPFHSSYLRNGVKPFKNFLKKNIVKENVKADRLIGKYIPNLTAKPFQITKEYFQDVFELTGSEKVKEILENWEKYQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFX5</accession>
    <name>C5DFX5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0D00726p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0D00726g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552755.1">
        <property type="nucleotide sequence ID" value="XM_002552709.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFX5"/>
    <dbReference type="FunCoup" id="C5DFX5">
        <property type="interactions" value="200"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFX5"/>
    <dbReference type="GeneID" id="8294975"/>
    <dbReference type="KEGG" id="lth:KLTH0D00726g"/>
    <dbReference type="eggNOG" id="ENOG502RUKY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023935_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFX5"/>
    <dbReference type="OMA" id="TQFPTPY"/>
    <dbReference type="OrthoDB" id="4063682at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="136"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="366"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="409"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="136"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="287"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="313"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="432"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="452"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="470" mass="51354" checksum="6C29A37FE12F9E86" modified="2009-07-28" version="1">MEPSNDEEAFFRVISENMKYAFTSPIATTTQFPTPYSQAHNNTSSHTQETERARTHSESQSIVENSMLAESNGASSLSDVNVQPSSVLQLGNEFMLTSPEQFREFLFESPAGINLLHRTPAKTPLRFFNSSAIVSSAQSQSHQSQQNQGHLPQQATNGPLQNQITPLRNIDVNLMFNSRNKQTNNTSSPSKRYLSLTPYGKRVLSDIGTPYAKLLASSNSALVDFQRARKDVQKSVGPQGSDLRSRGNSNTNKGLSGDLDVGSGHHKASLVSGSDDEIESGADDCGSSPTTIQLNSSVTKSTRDKLGSIGSIPENNITNSDLGPSSEDEHETNIDERLFEMEKLPLSPTPKPSKRNDLDVTRIKIPELPKMGSFKSERSSSVPNTAKKTTRKQPKFQIIVTDANSFNSTRGTVMGGDAVGKKRKPALKRSQSEIVSTTSASSSRGKTKRQKLSQVHNFSLDLNSRYPDSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DG53</accession>
    <name>C5DG53_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0D02486g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552833.1">
        <property type="nucleotide sequence ID" value="XM_002552787.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DG53"/>
    <dbReference type="FunCoup" id="C5DG53">
        <property type="interactions" value="457"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DG53"/>
    <dbReference type="GeneID" id="8295053"/>
    <dbReference type="KEGG" id="lth:KLTH0D02486g"/>
    <dbReference type="eggNOG" id="KOG1152">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004134_1_0_1"/>
    <dbReference type="InParanoid" id="C5DG53"/>
    <dbReference type="OMA" id="MDESECR"/>
    <dbReference type="OrthoDB" id="10252171at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045719">
        <property type="term" value="P:negative regulation of glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060917">
        <property type="term" value="P:regulation of (1-&gt;6)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14004">
        <property type="entry name" value="STKc_PASK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000320">
        <property type="entry name" value="Serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000314">
        <property type="entry name" value="Serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF51">
        <property type="entry name" value="PAS DOMAIN-CONTAINING SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="963"/>
            <end position="1221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="147"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="195"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="537"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="834"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="916"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="29"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="539"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="856"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="916"/>
            <end position="934"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR22395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1223" mass="135744" checksum="2C346C7AFD7448B0" modified="2009-07-28" version="1">MPCTSPPLSPTKNEQVEREAVRMAAQNKVKADETDEKQHANNSGGMGDYKPTGALGGAANLGPLSTQPLSSSDSDVRELLTFPNESTHAYSYNPLSPNSLAVRLRILKRSLEIMIRNPTLLQENRSGRHLRVETPNGTQKEFRFSTDSAISPKKRPQFSRSYSHEGVTPLSTSKLRNASSAALSALVTNAKGPSVAELSESSQPWTRPIAPFHSSQTSPRSAAGRFASPYQRVASAEGPSRAQRRSLDANADPNSMPQMNEVEPFYKTGNNTSAELIDAKRSELESLLLLLNETLENNSSEKASDLHMISLLNFNKLSLDMDHEEKDKDDASQHSSRYEQTLKRSLLESLAQPFFESSNTASTAGFDESGTLQGQDSFILPDSSFADMRTAPVSNRLLHTFVSSKNSSPQAIFTCLQQYPWQFKSANDLACLTFGISQNALKALTLLDLIHTDSRNFVLHKIMSTEGQELVFTGEIVAIVQPGESSNDLVWSSVWAKRRDGMIFCVFEKVPCDFMDVLLDLDDFSVEHISGGAGLKGLKQQTSNRGNQFSSVEKEKGGKKTVKFPNEVHDVADISISLKKLIEDVESGKVRSVDDDLLPMSIRVSNTINNVRYFTLNHLSLNIPCAISSSILENEMKLKIHSLPYQAGIFILDSNNFSMISFNKSISKNMFGMHASELVGQSICKVIPSFAELVSFIKIKYPALDITLQKNRGLVLTEHFFRKMQAEMSNNTESFFESIGIDAIHRDGSVLKVDIQLRVINSSNSLLWITHSRDVVFENYKTNPSQLLMLKESEVALVGSGTSSRSSMKAPSGTIHVDDLPNLKDSLKLDGSTVGSFDSASGDSRDNMEEPPDTPTEDKKQQDSDKLEMHKLGDQISAKFDVAKNYVQDKASFVKDKNFKLDENLIMSLKSLSPNKGNGSLASTSPPVPNSSSEEAGGQGEVFLSTPEPNIGSLKHVKKFSEFEVLQKMGEGAYGKVDLCLHKKDRYIVVIKLIFKERILVDTWVRDRKLGTIPSEIQIMATLNTKPHENILMLLDFFEDDEYYYIETPIHGTSGSVDLFDLIELKTDMTEHEAKLIFKQIVSGIKHLHDNGIVHRDIKDENVIVDSNGFVKIIDFGSAAYVKSGPFDVFVGTIDYAAPEVLGGEPYEGKPQDIWAIGVLLYTIIFKENPFYNIDEILDADLRVDSNHRVSDECIALIRKILNRPISKRPSISKINDDKWLEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DG59</accession>
    <name>C5DG59_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0D02618p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0D02618g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552839.1">
        <property type="nucleotide sequence ID" value="XM_002552793.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DG59"/>
    <dbReference type="FunCoup" id="C5DG59">
        <property type="interactions" value="80"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DG59"/>
    <dbReference type="GeneID" id="8295059"/>
    <dbReference type="KEGG" id="lth:KLTH0D02618g"/>
    <dbReference type="eggNOG" id="ENOG502RZK4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053570_2_1_1"/>
    <dbReference type="InParanoid" id="C5DG59"/>
    <dbReference type="OMA" id="ELMGTRH"/>
    <dbReference type="OrthoDB" id="244190at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15859">
        <property type="entry name" value="SNARE_SYN8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF423">
        <property type="entry name" value="SYNTAXIN-8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="252"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="2">
        <location>
            <begin position="176"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR22401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="271" mass="30444" checksum="C3F25DEDB293E18B" modified="2009-07-28" version="1">MEVLKISYGVEKLQGLVDERRRLVSVLKMKPSTNDTINMKRQMNAVLDSLQAAANAATSEDFVQLNALIEKYNDAVADIPDDSLDKELYKFASIDKPPETAISKLPEPKKVRFKDDVLEYDESTQDAPTAFAPYSDDVSDEERLEHDKTRLFQTGSSESTGLDGLAVAPQLSNQELFIQQQQQLLEQDSHLDNLSRSVHSSHVLSLDIHHEVADQNDGVLQDLESLVNNSGRNLDRAKKRLRIFENTARENGPCFIIVVLFVILIVLLIIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DG74</accession>
    <name>C5DG74_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">mRNA 3'-end-processing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0D02948g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the cleavage factor I (CF I) involved in pre-mRNA 3'-end processing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the CPSF4/YTH1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552854.1">
        <property type="nucleotide sequence ID" value="XM_002552808.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DG74"/>
    <dbReference type="FunCoup" id="C5DG74">
        <property type="interactions" value="101"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DG74"/>
    <dbReference type="GeneID" id="8295074"/>
    <dbReference type="KEGG" id="lth:KLTH0D02948g"/>
    <dbReference type="eggNOG" id="KOG1040">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024513_1_2_1"/>
    <dbReference type="InParanoid" id="C5DG74"/>
    <dbReference type="OMA" id="SLVCKHY"/>
    <dbReference type="OrthoDB" id="1914176at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000012">
        <property type="entry name" value="cleavage and polyadenylation specificity factor subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045348">
        <property type="entry name" value="CPSF4/Yth1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23102:SF24">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23102">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR SUBUNIT 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 11" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 10 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="12">
        <location>
            <begin position="28"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="12">
        <location>
            <begin position="61"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="12">
        <location>
            <begin position="89"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="12">
        <location>
            <begin position="118"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="12">
        <location>
            <begin position="147"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="10">
        <location>
            <begin position="28"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="10">
        <location>
            <begin position="61"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="10">
        <location>
            <begin position="89"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="10">
        <location>
            <begin position="118"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="10">
        <location>
            <begin position="147"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU369008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR22416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="210" mass="24661" checksum="D2F552B4BB91A05F" modified="2009-07-28" version="1">MSRIHPDTSNYPFRFEPFLRQEYSFSLDPDRPVCEFYNPREGPSSCPNGNSCPNKHVLPIFQNKIVCKHWLRGLCKKNDQCEYLHEYNLRKMPECVFFSKNGYCTQSPECQYLHINPSSKIQECEDYRMGFCPAGAQCKKRHIKKTMCPRYITGFCPLGRLDCEMEHPPFVIPNELSKLRIKNDDEINTKKQDEEKERRLNAIINGEIIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5DG82</accession>
    <name>C5DG82_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitochondrial import inner membrane translocase subunit TIM16</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Mitochondrial import inner membrane translocase subunit tim16</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12 13">Presequence translocated-associated motor subunit PAM16</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0D03124g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. In the complex, it is required to regulate activity of mtHSP70 (SSC1) via its interaction with PAM18/TIM14. May act by positioning PAM18/TIM14 in juxtaposition to mtHSP70 at the translocon to maximize ATPase stimulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TIM16/PAM16 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552862.1">
        <property type="nucleotide sequence ID" value="XM_002552816.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DG82"/>
    <dbReference type="FunCoup" id="C5DG82">
        <property type="interactions" value="272"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DG82"/>
    <dbReference type="GeneID" id="8295082"/>
    <dbReference type="KEGG" id="lth:KLTH0D03124g"/>
    <dbReference type="eggNOG" id="KOG3442">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101461_0_1_1"/>
    <dbReference type="InParanoid" id="C5DG82"/>
    <dbReference type="OMA" id="RMFKIND"/>
    <dbReference type="OrthoDB" id="10262892at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000006">
        <property type="entry name" value="Import inner membrane translocase subunit TIM16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005341">
        <property type="entry name" value="Tim16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12388">
        <property type="entry name" value="MITOCHONDRIA ASSOCIATED GRANULOCYTE MACROPHAGE CSF SIGNALING MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12388:SF0">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03656">
        <property type="entry name" value="Pam16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="116"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR22424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="135" mass="14871" checksum="3ECF8B57D9E48C10" modified="2009-07-28" version="1">MAHRALFQVIFTGAQVFGRAFSEAYKQAAVQATKTGANAARAGAAKAEYGGITLDESCKILNIENEADMASPEKIEERFKYLFDVNDKEKGGSFYLQSKIYRAAERLKYELAEKQQVDKAAEGQKGPEKPPSAEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGB8</accession>
    <name>C5DGB8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 17">Delta-aminolevulinic acid dehydratase</fullName>
            <ecNumber evidence="5 17">4.2.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ordered locus">KLTH0D04026g</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 17">
            <text>2 5-aminolevulinate = porphobilinogen + 2 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58126"/>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <dbReference type="EC" id="4.2.1.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 17">Homooctamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the ALAD family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.24" evidence="5 17"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552898.1">
        <property type="nucleotide sequence ID" value="XM_002552852.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGB8"/>
    <dbReference type="FunCoup" id="C5DGB8">
        <property type="interactions" value="670"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGB8"/>
    <dbReference type="GeneID" id="8295123"/>
    <dbReference type="KEGG" id="lth:KLTH0D04026g"/>
    <dbReference type="eggNOG" id="KOG2794">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035731_0_1_1"/>
    <dbReference type="InParanoid" id="C5DGB8"/>
    <dbReference type="OMA" id="YQMDYAN"/>
    <dbReference type="OrthoDB" id="1530at2759"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00318"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004655">
        <property type="term" value="F:porphobilinogen synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000048">
        <property type="entry name" value="Delta-aminolevulinic acid dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001731">
        <property type="entry name" value="ALAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030656">
        <property type="entry name" value="ALAD_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006762">
        <property type="entry name" value="PRK09283.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11458">
        <property type="entry name" value="DELTA-AMINOLEVULINIC ACID DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11458:SF0">
        <property type="entry name" value="DELTA-AMINOLEVULINIC ACID DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00490">
        <property type="entry name" value="ALAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001415">
        <property type="entry name" value="Porphbilin_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00144">
        <property type="entry name" value="DALDHYDRTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01004">
        <property type="entry name" value="ALAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00169">
        <property type="entry name" value="D_ALA_DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="10 17" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 17" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 16" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="14">
        <location>
            <position position="210"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="14">
        <location>
            <position position="263"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="329"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00020771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU004161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAR22460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="340" mass="37544" checksum="7BF8A481FA2B4060" modified="2009-07-28" version="1">MHTAEFLDAHPTEISSILAGGYNHPLLREWQNERQLTKNMFIFPLFISDDADEETPIDSLPNIKRFGVNRLAQYLAPLIEKGLKAVILFGVPMKEGCKDQYGTAADDPEGPVIQGINLLRREFPDLYIICDVCLCEYTSHGHCGVLYDDGSINREESVRRIAAVAVNYAKAGAHSIAPSDMIDGRIRDIKYGLIKAGLAHKTFVMSYAAKFSGNLYGPFRDAACSSPSSGDRKCYQLPSGGRGLARRALRRDLDEGTDGIIVKPSTFYLDIMADASEIARDVPICAYHVSGEYAMLHAAAEKGVVDFKTIAFESHQGFLRAGARLIISYLTPEFLEWLGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGG1</accession>
    <name>C5DGG1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">Glucose-6-phosphate 1-epimerase</fullName>
            <ecNumber evidence="3 5">5.1.3.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0D05060g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the interconversion between the alpha and beta anomers from at least three hexose 6-phosphate sugars (Glc6P, Gal6P, and Man6P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>alpha-D-glucose 6-phosphate = beta-D-glucose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:16249"/>
            <dbReference type="ChEBI" id="CHEBI:58225"/>
            <dbReference type="ChEBI" id="CHEBI:58247"/>
            <dbReference type="EC" id="5.1.3.15"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the glucose-6-phosphate 1-epimerase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.15" evidence="3 5"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552941.1">
        <property type="nucleotide sequence ID" value="XM_002552895.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGG1"/>
    <dbReference type="FunCoup" id="C5DGG1">
        <property type="interactions" value="300"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGG1"/>
    <dbReference type="GeneID" id="8295169"/>
    <dbReference type="KEGG" id="lth:KLTH0D05060g"/>
    <dbReference type="eggNOG" id="KOG1594">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048345_1_0_1"/>
    <dbReference type="InParanoid" id="C5DGG1"/>
    <dbReference type="OMA" id="TQALHSY"/>
    <dbReference type="OrthoDB" id="1659429at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047938">
        <property type="term" value="F:glucose-6-phosphate 1-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09020">
        <property type="entry name" value="D-hex-6-P-epi_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008183">
        <property type="entry name" value="Aldose_1/G6P_1-epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025532">
        <property type="entry name" value="G6P_1-epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014718">
        <property type="entry name" value="GH-type_carb-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11122">
        <property type="entry name" value="APOSPORY-ASSOCIATED PROTEIN C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11122:SF13">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01263">
        <property type="entry name" value="Aldose_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016020">
        <property type="entry name" value="PHexose_mutarotase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="6">
        <location>
            <position position="159"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="264"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR016020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR016020-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR016020-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR22503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="296" mass="33679" checksum="E11C5F2238AB3D69" modified="2009-07-28" version="1">MSVEETDTEVILTHPENSKTFVKILKYGATVYSWSLEGQEQLWLSSAAKLDGSKPVRGGIPLVFPVFGKNEDDEFLSKLPQHGLARNSTWEFLGQVSANPPTVQFGLGPELANPELTKLWPMDYSLILTVELGSGHLKTHIEVSNTSSSQPLKFNWLFHTYLKIADIEDTLVSNLTGAKLYDQMLQESYVDRQPVVSFHEELDRIYQRVQEDRLIQVIDKGHPIHTVKRHNLPDVVTWNPWVEKSKGMADFEPKSGFKEMVCVESGHVHSLLELQPGQSWTAYQLLYKDELNYQVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGI3</accession>
    <name>C5DGI3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">KLTH0D05544p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0D05544g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the peptidase M18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552963.1">
        <property type="nucleotide sequence ID" value="XM_002552917.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGI3"/>
    <dbReference type="FunCoup" id="C5DGI3">
        <property type="interactions" value="249"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGI3"/>
    <dbReference type="MEROPS" id="M18.001"/>
    <dbReference type="GeneID" id="8295191"/>
    <dbReference type="KEGG" id="lth:KLTH0D05544g"/>
    <dbReference type="eggNOG" id="KOG2596">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019532_3_0_1"/>
    <dbReference type="InParanoid" id="C5DGI3"/>
    <dbReference type="OMA" id="DWPIAKI"/>
    <dbReference type="OrthoDB" id="9880441at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05658">
        <property type="entry name" value="M18_DAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.250.10:FF:000001">
        <property type="entry name" value="Aspartyl aminopeptidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.250.10">
        <property type="entry name" value="Aminopeptidase i, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001948">
        <property type="entry name" value="Peptidase_M18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023358">
        <property type="entry name" value="Peptidase_M18_dom2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28570">
        <property type="entry name" value="ASPARTYL AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28570:SF4">
        <property type="entry name" value="VACUOLAR AMINOPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02127">
        <property type="entry name" value="Peptidase_M18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00932">
        <property type="entry name" value="AMINO1PTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101821">
        <property type="entry name" value="Aminopeptidase/glucanase lid domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4 9" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="232"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR22525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="511" mass="55775" checksum="741082920098E7B6" modified="2009-07-28" version="1">MSDATQEALAQIKKTLELLALQKPTTGEASQPAAVAHNSSQKYRGKDYFEESSKSYINFTYESPTIYHVVEYFSRKLDEAGFSYVSEKSTWSEVKSGKYYTIRNGTNLVAFILGSDWKYETGVGAIGSHIDALCAKLKPVSTKKDVEGFQLLGVAPYGGTLNTYWFDRDLGIGGRVLVRDPDSGKIESRLINSAPHPIAKIPSLAVHFGEPANGPFDKEDQAVPVIGYSGTGDDDEEGDSEDEKRSPLFGKHPIGLLRYIASLAKVKVSQLVQVDLDLFDVQKGTLGGLKNDFLFAPRLDDRLCSFSAVNSLIDFANDGPVPRDAFSIVALFDDEEVGSLSRQGARGGLLESVVQRVVANLHGDSPELLRTTFANSLIISADVNHMFNPNFSSVYLEHHRPKPNVGVTLSLDSNLHMSTDVVGLAVAEELARLNGDKLQYFHIKNNSRSGGTIGPFISSQTGARTIDLGIAQLSMHSIRAATGSKDIGLAIKFFKGFFANWRSVYDKFGDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGJ5</accession>
    <name>C5DGJ5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0D05808p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0D05808g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552975.1">
        <property type="nucleotide sequence ID" value="XM_002552929.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGJ5"/>
    <dbReference type="FunCoup" id="C5DGJ5">
        <property type="interactions" value="96"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGJ5"/>
    <dbReference type="GeneID" id="8295203"/>
    <dbReference type="KEGG" id="lth:KLTH0D05808g"/>
    <dbReference type="eggNOG" id="KOG3508">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002973_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGJ5"/>
    <dbReference type="OMA" id="WFVGLNY"/>
    <dbReference type="OrthoDB" id="775356at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00030">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05137">
        <property type="entry name" value="RasGAP_CLA2_BUD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.506.10">
        <property type="entry name" value="GTPase Activation - p120gap, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039360">
        <property type="entry name" value="Ras_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023152">
        <property type="entry name" value="RasGAP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001936">
        <property type="entry name" value="RasGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194:SF60">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEIN RASKOL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00616">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00323">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00509">
        <property type="entry name" value="RAS_GTPASE_ACTIV_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50018">
        <property type="entry name" value="RAS_GTPASE_ACTIV_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ras-GAP" evidence="3">
        <location>
            <begin position="525"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="997"/>
            <end position="1053"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="22"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1005"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1040"/>
            <end position="1050"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR22537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1060" mass="120161" checksum="65B3EC8B4B0C6445" modified="2009-07-28" version="1">MGTSQFNISEPTPLREGPFSNGYASSGSDQFSGMNSTESLLISNIQSNRGQFKGEVQWCSNLSLDDWKTHFLQIDERGALCHALNEEGIADLRVVTEKPASSNKPILKHLQSCKVQLLDSSMGNLSVIKVETYYSTIYLRATEKKTFLSLFSALIFWKSLKDRGIFNKISVIQPIFSRQQKPIDLLVCQFNIYGPIPRNKNVPLKSGLATPPPIAGVSVIDEMGWYSAMGRLSSDGRLDLLLQSDGSVLWSLDISSLLRSEIRILDPSLFQNDNFLFLGVVRRLRDQLKISSNIPFYSSTTKTQVNSQAIYLQFPLRIDLEDWFVALNTFAQVESVSLLGTDKSNELRISNRFKVSILEADFSGINLMSYDHERHQEILPRLYSDISIWDHCWARTSIVTDAQTPFWREEFDFNFSVKTSPVCVAVKIALGDDGEYSSSDKTLGVIEITQEMINDGSLSAETRIPLFDAENKHFQLGTLCIKVASSLNAILPSVNFTKLDSMLSRMSLTKMTEYLNDISIADYLKLEDLSVVFLDIFQALNRTNDWFQALIEKEFSNIDNSISKNTNKNLSSANIYSSLFRGNSILTKSIERYFNRIGQEYLDKAIGGTIRKVVSSDMCLELDPSRIREIDAQRKSEILEGNYSRLLKVAEELWSRISSTSNDLPIGIKEQLKAFRKNLEIICKNDELKSTLNCISGFLFLRFFCPVILNPKLFHIVENHPEEGPKRTLTLLTKIILNLSTLTLFGKKEPWMTKMNEFILKHEGELTDYIDKVTDKKLDFSPKALKLSNSVARPRVALNESCLNDLPTNPYLIDRYLRETELISVLTIFRRDEEKSMTVPKTVSMNRLSKEPSSSFPSPIIDQTKATIGELEFEKLTENNAEVFGEDLMKYLNIDKANDNVGSESPTVAGFTIEEKDLTKTLEQESLLLYHRLEHLTNVLSNYEYPNEIILGKTEFALFLVDNLYMDRNNIVELDLSGSFAKQNGLSKLCSDSSLFMKDSDRHQSPPNSGQISRARTVSQRPNLGFSSSKSNPLSKKKGKQSENKSDNKQGFKLLRFFRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGK4</accession>
    <name>C5DGK4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">Ribosome assembly factor mrt4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0D06050g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">Component of the ribosome assembly machinery. Nuclear paralog of the ribosomal protein P0, it binds pre-60S subunits at an early stage of assembly in the nucleolus, and is replaced by P0 in cytoplasmic pre-60S subunits and mature 80S ribosomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 7">Associates with the pre-60S ribosomal particle.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
            <location evidence="7">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the universal ribosomal protein uL10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552984.1">
        <property type="nucleotide sequence ID" value="XM_002552938.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGK4"/>
    <dbReference type="FunCoup" id="C5DGK4">
        <property type="interactions" value="1175"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGK4"/>
    <dbReference type="GeneID" id="8295214"/>
    <dbReference type="KEGG" id="lth:KLTH0D06050g"/>
    <dbReference type="eggNOG" id="KOG0816">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071690_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGK4"/>
    <dbReference type="OMA" id="LEWAENY"/>
    <dbReference type="OrthoDB" id="10262308at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05796">
        <property type="entry name" value="Ribosomal_P0_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1730:FF:000005">
        <property type="entry name" value="Ribosome assembly factor mrt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.105.20:FF:000003">
        <property type="entry name" value="Ribosome assembly factor mrt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.105.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033867">
        <property type="entry name" value="Mrt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001790">
        <property type="entry name" value="Ribosomal_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040637">
        <property type="entry name" value="Ribosomal_uL10-like_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043164">
        <property type="entry name" value="Ribosomal_uL10-like_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043141">
        <property type="entry name" value="Ribosomal_uL10-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051742">
        <property type="entry name" value="Ribosome_Assembly_uL10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45841:SF1">
        <property type="entry name" value="MRNA TURNOVER PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45841">
        <property type="entry name" value="MRNA TURNOVER PROTEIN 4 MRTO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00466">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17777">
        <property type="entry name" value="RL10P_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160369">
        <property type="entry name" value="Ribosomal protein L10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL10-like insertion" evidence="8">
        <location>
            <begin position="130"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR22546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="238" mass="27050" checksum="40EF3EB5AEAE075E" modified="2009-07-28" version="1">MPRSKRSKLVTLAQTDKKGRENKERIFDEVREALDTFRFVWVLHLDDVRTPVLQEIRSAWVGSKLIMGKRKVLQKSLGEKREEEYKENIFALSKLCSGVTGLLFTDEEPQVVQEYFKSYVRADYSRPKSKAPLTFEIPAGIIYSRGGQIPAEDDVPMVHSLEPTLRNKFMIPTKIKAGKITIESPYLVCTEGETLDVRQALILKQFGVAAAEFRVKVAAYYDNETSSVETVNINIESA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGT4</accession>
    <name>C5DGT4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0D08074p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0D08074g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. TrmE GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553064.1">
        <property type="nucleotide sequence ID" value="XM_002553018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGT4"/>
    <dbReference type="FunCoup" id="C5DGT4">
        <property type="interactions" value="483"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGT4"/>
    <dbReference type="GeneID" id="8295297"/>
    <dbReference type="KEGG" id="lth:KLTH0D08074g"/>
    <dbReference type="eggNOG" id="KOG1191">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019624_3_1_1"/>
    <dbReference type="InParanoid" id="C5DGT4"/>
    <dbReference type="OMA" id="EFHCHGG"/>
    <dbReference type="OrthoDB" id="188276at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04164">
        <property type="entry name" value="trmE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14858">
        <property type="entry name" value="TrmE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.120">
        <property type="entry name" value="Probable tRNA modification gtpase trme, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.430">
        <property type="entry name" value="tRNA modification GTPase MnmE domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00379">
        <property type="entry name" value="GTPase_MnmE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031168">
        <property type="entry name" value="G_TrmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018948">
        <property type="entry name" value="GTP-bd_TrmE_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004520">
        <property type="entry name" value="GTPase_MnmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027368">
        <property type="entry name" value="MnmE_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025867">
        <property type="entry name" value="MnmE_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027266">
        <property type="entry name" value="TrmE/GcvT-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00450">
        <property type="entry name" value="mnmE_trmE_thdF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003661">
        <property type="entry name" value="PRK05291.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42714">
        <property type="entry name" value="TRNA MODIFICATION GTPASE GTPBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42714:SF2">
        <property type="entry name" value="TRNA MODIFICATION GTPASE GTPBP3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12631">
        <property type="entry name" value="MnmE_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10396">
        <property type="entry name" value="TrmE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116878">
        <property type="entry name" value="TrmE connector domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51709">
        <property type="entry name" value="G_TRME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="TrmE-type G" evidence="6">
        <location>
            <begin position="248"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR22626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="497" mass="54374" checksum="C63B0825F4EC4FF1" modified="2009-07-28" version="1">MQRGLYASYRLYSSVPATAFHPTIYALSTSPGQRSAIAVVRVTGSHCKHIYYQLTGAQSPPVPRRCSLRNLYHAAGAKKNLLDSSLVLFFENPKSFTGEDMLELHLHGGRAVIKSVLGAIQSLGDQKKGLDIRYAQPGEFSQRAFQNGRMDLTQAEGVADLIDAETETQRRSALQSFRGQNKALFDGWRSQIVSGIAQLTAIIDFGEDAEIEDTQAILDSVRRNMMNLDKEIKLFVLKIRRSSILQNGVKVALLGSPNAGKSSLLNCITNDDTSIVSDTPGTTRDAIDVPIDVDGYKVVLCDTAGIRSESEDQIEIQGIKRAKAKGSESDLVILVIDPTKTPLVTEDLQRFVKEQIPHNQVIIAVNKTDLVDTKGLKNVRQEVYQIFNGDFPIKSVSCTNFEGIENLVKELSHVFQGLSGTSHDSDPLIVSNRTEEILTKDVMFGLQEFLSFPGTEDVVMACENLAYAAEGIGKITGASVGVEEVLGVVFSRFCVGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>C5DH58</accession>
    <name>C5DH58_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="20">KLTH0E01540p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="ordered locus">KLTH0E01540g</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ferric reductase (FRE) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553556.1">
        <property type="nucleotide sequence ID" value="XM_002553510.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DH58"/>
    <dbReference type="GeneID" id="8291694"/>
    <dbReference type="KEGG" id="lth:KLTH0E01540g"/>
    <dbReference type="eggNOG" id="KOG0039">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010365_4_0_1"/>
    <dbReference type="InParanoid" id="C5DH58"/>
    <dbReference type="OMA" id="GWSYRTF"/>
    <dbReference type="OrthoDB" id="4494341at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000293">
        <property type="term" value="F:ferric-chelate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015677">
        <property type="term" value="P:copper ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06186">
        <property type="entry name" value="NOX_Duox_like_FAD_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013112">
        <property type="entry name" value="FAD-bd_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013121">
        <property type="entry name" value="Fe_red_NAD-bd_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051410">
        <property type="entry name" value="Ferric/Cupric_Reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361:SF9">
        <property type="entry name" value="FERRIC REDUCTASE TRANSMEMBRANE COMPONENT 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361">
        <property type="entry name" value="FERRIC/CUPRIC REDUCTASE TRANSMEMBRANE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08022">
        <property type="entry name" value="FAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08030">
        <property type="entry name" value="NAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00052">
        <property type="entry name" value="Ferric_Reductase_Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01168">
        <property type="entry name" value="Ferric_reductase_subgroup_(FRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0349">Heme</keyword>
    <keyword evidence="14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13" id="KW-0408">Iron</keyword>
    <keyword evidence="15 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0521">NADP</keyword>
    <keyword evidence="12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="18" id="KW-0732">Signal</keyword>
    <keyword evidence="7 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002948733" evidence="18">
        <location>
            <begin position="19"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="156"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="232"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="272"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="311"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="345"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="374"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="19">
        <location>
            <begin position="408"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CAR23119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="711" mass="81544" checksum="95D0743AE38EEE6B" modified="2009-07-28" version="1">MKFLFTAVLATVMTAVSAELGYQYDEVDYVGFSCKRALSVTAKFCKVDTATSYDCYCEEPNAIASFFSCTNENLKSSKNRKAMEDFFQFRCPNITDSEMRSVTQNLSSSLVNTSLISGFNKTKPVTYPVYANKTAYQISYDSYKTRYSQFRMGQHYGAALVGYWGIIMVCGMLSNLFCKFAPNLALSLNKKSSQLAPVRLYRKHISIPATFRSKHTTRNFVKGLIPTRLESLIIFGFVVLCVIFHAVNFHSVPNNILFTTKPLEISRHVGDRSAMLANYLMTLTFLLAGRNQIFITLTGWKQSTFMTYHKWVGRMLFCSVLVHTISMFTYSLMRDYYTYWAAEEWWKYGAVAVVAGGIIAIQSFSWFREWNYELFLYVHIATAIAFLVGAWRHIHYFHYAEWAYATAAVWCFDRFVRLVRIFMFGVKTAKVGIVSEETLVITVNKGSWWPFFPGAFGYLHIMKLNIFWQSHPFTVVKTDNNELRFFIKIKRGATKKLYNQLLKEPNHESEIKVALEGPYGDKKPVEAYDQVLLYSGGNGIPGPFAYAKELGEISKEAKSKFVKLYWVIRHWNSLDWFLDELMILQRFSNVQTIIYVTRADDGKVGEKLAHHFDQGESESSEGEKGSNVVATTISDYAETMQKALPHIEFRKGRPDIAALVEQDIDECHDQNAAIITCGHNEMCDRIRQTVAVKVGDHKTSRIDFFEELQTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHD3</accession>
    <name>C5DHD3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0E03432p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0E03432g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553631.1">
        <property type="nucleotide sequence ID" value="XM_002553585.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHD3"/>
    <dbReference type="GeneID" id="8291779"/>
    <dbReference type="KEGG" id="lth:KLTH0E03432g"/>
    <dbReference type="eggNOG" id="ENOG502S7Z4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_402279_0_0_1"/>
    <dbReference type="InParanoid" id="C5DHD3"/>
    <dbReference type="OMA" id="ESTMVPR"/>
    <dbReference type="OrthoDB" id="8059989at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051092">
        <property type="entry name" value="FYVE_RhoGEF_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12673">
        <property type="entry name" value="FACIOGENITAL DYSPLASIA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12673:SF159">
        <property type="entry name" value="LD03170P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="2">
        <location>
            <begin position="56"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR23194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="733" mass="83014" checksum="6E8D5DD05B0E080F" modified="2009-07-28" version="1">MATQMESPLLDPLSGVDRESQSSNMASPVQFIKEYIERTLNTPPGFPVSVAAEHYKRQRLVEEIVESETEYVQMLKILRNCFLDVLSINDYIPMKVLNNVVGQILAHHEELCRCLQAVVDGSVKRFDETTELNWSDLSLTSWDHLEVCQQVAEILSTHAVQAKQYCSYLYYYNQISHLLARMGRESEDGLLFAILFRIELCLPKYYSHTSHQGYKRDFSFRAMIQMPINRIAKYRLFLESLVYLTETPSITDLRRLDETKCQALIHSIKTSLGSMYSNLQKVDEANCEKLPDPALVLVYNRLTFSSPDERIPVEAMGNCYTRGCLAVAWTCMDEAAHSAGSHRKSSARYFNNFHRAKRSLRIESEQLGVFLFKSYLIFAEIPSFKLGTASYEWPIKFAIRLASCRLMDARPAVQRALQGGMKDCGIYSEYENAIKLQFEYDFKLWEILLLCWDSQELEAWRKELSLFINEINGPHTFGVDPLRETVSQSALESTLHFSDSTTFPDQMLPFMVYNENLSGRFDRTRAFKRGLSTRAGQCYEGQTVEVAVEFRNADVDMSPSSTWFLRGSSSRVNCTTLASSSTCAFVEASTGVSSTPDAEAISINERCREQRDSSAPCAVLSRWRRGEIERAMRDVWSSHLNLQAADIITTVAGASTGTSIYDDTLGSLRALLRTASDNCVAVTGTQSTPVKSSCAQPPAPPARAATRWSIKRVFSRRRRGSQSVVRHQNALKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHG2</accession>
    <name>C5DHG2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0E04092p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0E04092g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF9 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553660.1">
        <property type="nucleotide sequence ID" value="XM_002553614.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHG2"/>
    <dbReference type="FunCoup" id="C5DHG2">
        <property type="interactions" value="597"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHG2"/>
    <dbReference type="GeneID" id="8291809"/>
    <dbReference type="KEGG" id="lth:KLTH0E04092g"/>
    <dbReference type="eggNOG" id="KOG3334">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068315_3_1_1"/>
    <dbReference type="InParanoid" id="C5DHG2"/>
    <dbReference type="OMA" id="PHDAQVM"/>
    <dbReference type="OrthoDB" id="341924at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07979">
        <property type="entry name" value="HFD_TAF9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000064">
        <property type="entry name" value="Transcription initiation factor TFIID subunit 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003162">
        <property type="entry name" value="TFIID-31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051431">
        <property type="entry name" value="TFIID_subunit_9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48068">
        <property type="entry name" value="TAF9 RNA POLYMERASE II, TATA BOX-BINDING PROTEIN (TBP)-ASSOCIATED FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48068:SF4">
        <property type="entry name" value="TATA-BOX BINDING PROTEIN ASSOCIATED FACTOR 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02291">
        <property type="entry name" value="TFIID-31kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR23223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="146" mass="16575" checksum="47991B0D088FE0CC" modified="2009-07-28" version="1">MTDDAQEEVPRDVRLLHLLLASQAIQHYEDQVPLQLMDFAHRYTRGVLKDAMVYGDYAAGDAAAELSVEDIRLAIAARTQYQFKPTAPKELLLQLAAERNKKALPQVVNMWGTRLPPEKYCLTSREWRLEEERDEQGHAVGDGAAR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-05-31" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHJ9</accession>
    <name>STS1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName>Tethering factor for nuclear proteasome STS1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">STS1</name>
        <name type="ordered locus">KLTH0E04906g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in ubiquitin-mediated protein degradation. Regulatory factor in the ubiquitin/proteasome pathway that controls the turnover of proteasome substrates. Targets proteasomes to the nucleus and facilitates the degradation of nuclear proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Binds the proteasome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cut8/STS1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553697.1">
        <property type="nucleotide sequence ID" value="XM_002553651.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHJ9"/>
    <dbReference type="SMR" id="C5DHJ9"/>
    <dbReference type="FunCoup" id="C5DHJ9">
        <property type="interactions" value="19"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHJ9"/>
    <dbReference type="GeneID" id="8291846"/>
    <dbReference type="KEGG" id="lth:KLTH0E04906g"/>
    <dbReference type="eggNOG" id="ENOG502RNK4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054606_1_0_1"/>
    <dbReference type="InParanoid" id="C5DHJ9"/>
    <dbReference type="OMA" id="DYTPHFL"/>
    <dbReference type="OrthoDB" id="10061064at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071630">
        <property type="term" value="P:nuclear protein quality control by the ubiquitin-proteasome system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031144">
        <property type="term" value="P:proteasome localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1590">
        <property type="entry name" value="Tethering factor for nuclear proteasome Cut8/Sts1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013868">
        <property type="entry name" value="Cut8/Sts1_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038422">
        <property type="entry name" value="Cut8/Sts1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28032">
        <property type="entry name" value="FI02826P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28032:SF1">
        <property type="entry name" value="FI02826P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08559">
        <property type="entry name" value="Cut8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0539">Nucleus</keyword>
    <keyword id="KW-0653">Protein transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000409413" description="Tethering factor for nuclear proteasome STS1">
        <location>
            <begin position="1"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="299" mass="33458" checksum="C2919D5151F6FF34" modified="2009-07-28" version="1">MSQSVGFNWGFKPSTAENTSSAISTCKVTKPRVKRRFAGEEAASGGSPTYNNRPRAVAKRRTAVSGIQGQPLPLSRALELMDRTQLQSTLLELVKLHPEIQSTFMALQPPSRGLDQYVTLLRGKLEQVYANLPYSRLRDRLPDDTGEGLNDYTFVRVKSFLLEFLNCLVDCLLESIPPQVQNPLHSLKLLDTATGLLSQLPHFSTASNNYYKNVCYEQVAEIWSTVIRQATQDISFLGATSTVLDWLQILQKHNEKSQGKLQRPLSLLQTFQESLEPVQAPAGISQDQQQPSNLWNNIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHK5</accession>
    <name>C5DHK5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 13">Deoxyuridine 5'-triphosphate nucleotidohydrolase</fullName>
            <shortName evidence="13">dUTPase</shortName>
            <ecNumber evidence="5 13">3.6.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 13">dUTP pyrophosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0E05038g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Involved in nucleotide metabolism via production of dUMP, the immediate precursor of thymidine nucleotides, and decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>dUTP + H2O = dUMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10248"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61555"/>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <dbReference type="EC" id="3.6.1.23"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:10249"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 13">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the dUTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.23" evidence="5 13"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553703.1">
        <property type="nucleotide sequence ID" value="XM_002553657.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHK5"/>
    <dbReference type="FunCoup" id="C5DHK5">
        <property type="interactions" value="1219"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHK5"/>
    <dbReference type="GeneID" id="8291852"/>
    <dbReference type="KEGG" id="lth:KLTH0E05038g"/>
    <dbReference type="eggNOG" id="KOG3370">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068508_2_1_1"/>
    <dbReference type="InParanoid" id="C5DHK5"/>
    <dbReference type="OMA" id="GREFHTQ"/>
    <dbReference type="OrthoDB" id="419889at2759"/>
    <dbReference type="UniPathway" id="UPA00610">
        <property type="reaction ID" value="UER00666"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004170">
        <property type="term" value="F:dUTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006226">
        <property type="term" value="P:dUMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046081">
        <property type="term" value="P:dUTP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07557">
        <property type="entry name" value="trimeric_dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.40.10:FF:000007">
        <property type="entry name" value="dUTP pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008181">
        <property type="entry name" value="dUTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029054">
        <property type="entry name" value="dUTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036157">
        <property type="entry name" value="dUTPase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033704">
        <property type="entry name" value="dUTPase_trimeric"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00576">
        <property type="entry name" value="dut"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001862">
        <property type="entry name" value="PRK00601.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11241">
        <property type="entry name" value="DEOXYURIDINE 5'-TRIPHOSPHATE NUCLEOTIDOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11241:SF0">
        <property type="entry name" value="DEOXYURIDINE 5'-TRIPHOSPHATE NUCLEOTIDOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00692">
        <property type="entry name" value="dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51283">
        <property type="entry name" value="dUTPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 13" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="dUTPase-like" evidence="14">
        <location>
            <begin position="27"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00021732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU367024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR23266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="156" mass="16353" checksum="316CBBEC47EC3B4C" modified="2009-07-28" version="1">MTNIDAPARKQQKLNNSLLIQLRSPDAIAPTKGSASAAGYDIYASQGSVIPARGQGLVSTDVSFTVPVGTYGRIAPRSGLAVKHGINTGAGVVDRDYTGEVKIVLFNHADKDFEVRKGDRVAQLILEKIVDDAEIVVVDSLEESARGAGGFGSTGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHP8</accession>
    <name>C5DHP8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0E06072p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0E06072g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553746.1">
        <property type="nucleotide sequence ID" value="XM_002553700.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHP8"/>
    <dbReference type="FunCoup" id="C5DHP8">
        <property type="interactions" value="216"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHP8"/>
    <dbReference type="GeneID" id="8291899"/>
    <dbReference type="KEGG" id="lth:KLTH0E06072g"/>
    <dbReference type="eggNOG" id="ENOG502S1YQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051557_0_0_1"/>
    <dbReference type="InParanoid" id="C5DHP8"/>
    <dbReference type="OMA" id="APPDYWE"/>
    <dbReference type="OrthoDB" id="4034416at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="CDD" id="cd22571">
        <property type="entry name" value="SNF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059172">
        <property type="entry name" value="SNF6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="209"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="209"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="302" mass="34746" checksum="F54E04E7D902C27D" modified="2009-07-28" version="1">MPPRKRRTHNLKGLRGSQDQQQSHSSYFATRLKPEQVGALVHDESDTISYRAYLVQNFIRSSEMMDALMTQNVPLPKIKAPPIFRNPNVEEMKRAVVSEREQLDAIESHLQSYAIDLPDGYHQLKELSMQIDLSQDVSDNSIQAAESAYKAQTGKYTQDDRIVVHKDRFPQLRGDHSKAPPDYWERRLDILASQQQEALRLKIMQEEEEELKRRTEEENRLRQKQEEQRLYEDPFQQQATHAQPMAGPVTANPGIPDPQPAQQPSDQPPHAMLGSIFGDMNGENFNNGFEDDEFADLDTAFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHV8</accession>
    <name>C5DHV8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0E07524p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0E07524g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553806.1">
        <property type="nucleotide sequence ID" value="XM_002553760.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHV8"/>
    <dbReference type="FunCoup" id="C5DHV8">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHV8"/>
    <dbReference type="GeneID" id="8291963"/>
    <dbReference type="KEGG" id="lth:KLTH0E07524g"/>
    <dbReference type="eggNOG" id="ENOG502RZ92">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055503_0_0_1"/>
    <dbReference type="InParanoid" id="C5DHV8"/>
    <dbReference type="OMA" id="EYHDELC"/>
    <dbReference type="OrthoDB" id="5378975at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="240" mass="27331" checksum="30772D453FAF683B" modified="2009-07-28" version="1">MSDSDSDSDFGDFAGVPEEESPVPEILSPVEQLDRALGQREEIQYVNQPHSLDELIKGERPRVVYEHLVLLETQLRPFSWRNSKLRSLLLQTLQIADEPELPKAHKPLDSFLYSQLEPRLGEEGLDYAAFLSRVCGDKFSANNKSPVQKIESLEAKDLEQLDTEALRRTHDELLDAVLAVCQEIGGTAAVRSELEADKSMYEGLVTNLVGHTQRLRRDEIADFNKKHRRGSKYGKFKWVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHY0</accession>
    <name>C5DHY0_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Calcium-transporting ATPase</fullName>
            <ecNumber evidence="17">7.2.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ordered locus">KLTH0E08052g</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Catalyzes the hydrolysis of ATP coupled with the transport of calcium.</text>
    </comment>
    <comment type="function">
        <text evidence="16">This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium. Transports the calcium to the vacuole and participates in the control of the cytosolic free calcium.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 17">
            <text>Ca(2+)(in) + ATP + H2O = Ca(2+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18105"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Membrane</location>
            <topology evidence="17">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.10" evidence="17"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553828.1">
        <property type="nucleotide sequence ID" value="XM_002553782.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHY0"/>
    <dbReference type="FunCoup" id="C5DHY0">
        <property type="interactions" value="486"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHY0"/>
    <dbReference type="GeneID" id="8291987"/>
    <dbReference type="KEGG" id="lth:KLTH0E08052g"/>
    <dbReference type="eggNOG" id="KOG0204">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002360_9_3_1"/>
    <dbReference type="InParanoid" id="C5DHY0"/>
    <dbReference type="OMA" id="YRMYVKG"/>
    <dbReference type="OrthoDB" id="3352408at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005388">
        <property type="term" value="F:P-type calcium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02081">
        <property type="entry name" value="P-type_ATPase_Ca_PMCA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000028">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000018">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006408">
        <property type="entry name" value="P-type_ATPase_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01517">
        <property type="entry name" value="ATPase-IIB_Ca"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093:SF369">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093">
        <property type="entry name" value="CATION TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 17" id="KW-0106">Calcium</keyword>
    <keyword evidence="4 17" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="13 17" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 17" id="KW-0813">Transport</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="110"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="331"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="372"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="888"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="912"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1049"/>
            <end position="1073"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1080"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="19">
        <location>
            <begin position="48"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="495"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00059328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU361146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CAR23391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1238" mass="136011" checksum="68DAEB9EEA8B6332" modified="2009-07-28" version="1">MGEQSPSSKVDDASEHADSIKFRSSEFKLKPDQLAELHDPKSMAAFVNLFDEDPNNLYTFLGTDKGSGLNIADEDVTETERYKIYGDNRIPQRKPKSFLELAWEAFQDRIMILLTVAAVISFALGLYETLGQPPEYDSEGKEITKVDWVEGVAIMIAVLVVILVGSANDYQKELQFSKLNNKKNDREIIVLRNGDEHLISIHDILVGDIIVLQTGDVVPADCVLVKGSCECDESALTGESQTIKKASIDVACSKYKHLSANDAAIDIGVRAAEKVPDPMLISGSKLLSGLGRAVVTSVGVNSVHGRTLMSLKVENETTPLQERLDSLANSISVYGSVAALILFIVLFIRFLVNLKKGGHLSGLSPAQKGSRFMNIFIVAITIIVVAVPEGLPLAVTLALAFATTRMAKDGNLVRVLRACETMGSGTAVCSDKTGTLTENRMTVVKGFLGTTHFDETEETSEVVEPEDHIISKDCPQATKKDLLVNITLNSTAFENKDDDEGMESNENPFHKPRKTLFPWSRNNRKERHTTANELIENAPDQDREPFIGSKTETALLSFARKHLDMQDLQNYREHPENLDVAEVVQVIPFESVRKWGGVVLKYNDGTYRFFIKGAAELILKRCKQKRTSDGKSGSISEEDFESASRTITGLAADALRAISLAHRDFLNCQSWPPEELQDPADPSLALPDRLFGDEVSFLGSHTDPSGVPKEGLVLDAIVGIQDPLRKGVRVAVEQCQKAGVTVRMVTGDNILTATAIARNCSILQGGFADDPDSSMEGPKFRKLATKERRRIVPKLRVLARSSPEDKRILVETLKEMGDVVAVTGDGTNDAPALKLADVGFSMGISGTEVAREASDIILMTDDFSAIVNAIKWGRCVSTSIKKFIQFQLTVNITAVVLTFVSAVASAEETSVLTAVQLLWVNLIMDTLAALALATDKPDENILDRKPRGREAPLITVSTWKMILGQSTLQLIVTFVLHFAGKQIFFPHKAHITGHEQQQLNAMTFNTFVWLQFFKLIVTRKLDEADGISNVRDRITASNLNFFQDLFRNYYFLVIILLIGGFQVLIMFVGGAAFSIAKQTGAMWATAIICGMLSLPVGVIIRIIPDEWVLKVFPSRFVKMILYVLGFKFLRKSGNKNEEEESLLDSNSDAKLVGSTAFERAKTEMLAFKDHVGLDNNHLNPFQAYKKWRHSSSSSAGSDSEESHYYIASATMVPTLVGGAVGGFSHITMKDGPSDKKAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHY6</accession>
    <name>C5DHY6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0E08184p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0E08184g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553834.1">
        <property type="nucleotide sequence ID" value="XM_002553788.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHY6"/>
    <dbReference type="FunCoup" id="C5DHY6">
        <property type="interactions" value="1040"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHY6"/>
    <dbReference type="GeneID" id="8291993"/>
    <dbReference type="KEGG" id="lth:KLTH0E08184g"/>
    <dbReference type="eggNOG" id="KOG1273">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032142_0_0_1"/>
    <dbReference type="InParanoid" id="C5DHY6"/>
    <dbReference type="OMA" id="DYEDDIM"/>
    <dbReference type="OrthoDB" id="196858at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037850">
        <property type="entry name" value="RBBP5/Swd1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44040">
        <property type="entry name" value="RETINOBLASTOMA-BINDING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44040:SF1">
        <property type="entry name" value="RETINOBLASTOMA-BINDING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="69"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR23397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="437" mass="50209" checksum="425D40E8D94DDABF" modified="2009-07-28" version="1">MANLLLQDPFSVLKEYPERLTQTFETPFHTCCVQFSYAGDYLAMGCSNGAVMIYDMDTTKPIAMLGGTSGAHVRSIQSIAWSRSGRYVLTSSRDWFVKLWDMKTPGEPFRQVKFGSSPWTCQWTFLSEWKCVVTVYEETHAFVVDFAEDVPHARELLASTELQEPEEEGDQKERDYGYTLVVCSHPKHTSVIITGTSKGWLKAYLVDGEKTELVCSYRFAPTNAKHIIMSQSGDRMAINSSDRTIRQYLVTVSDDLKEINFTLEHKYQDVINKLQWNSIFFSSNSAEYLVASTHGSSAHDLYLWETSTGTLVRVLEGTDEELLDIDWNFYNMCIASNGLETGNVYIWSLVISPKWSALAPDFEEVEENIEYREKEDEFDQVGQLEQQQEMDQAEEVRIDLRCRERYDVRGNEIAVDRFVVPLDYESILVLKNWHKNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5DI32</accession>
    <name>C5DI32_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0E09306p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0E09306g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553880.1">
        <property type="nucleotide sequence ID" value="XM_002553834.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DI32"/>
    <dbReference type="FunCoup" id="C5DI32">
        <property type="interactions" value="168"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DI32"/>
    <dbReference type="GeneID" id="8292043"/>
    <dbReference type="KEGG" id="lth:KLTH0E09306g"/>
    <dbReference type="eggNOG" id="KOG3085">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1469337_0_0_1"/>
    <dbReference type="InParanoid" id="C5DI32"/>
    <dbReference type="OrthoDB" id="444127at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051828">
        <property type="entry name" value="HAD-like_hydrolase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46191">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46191:SF2">
        <property type="entry name" value="HALOACID DEHALOGENASE-LIKE HYDROLASE DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR23443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="207" mass="23823" checksum="0827FC45742DB4DF" modified="2009-07-28" version="1">MVDEILHVFEGFGAYTVYPDLLELLELIQQEHSEVVLGVVSNTDPIMYKLLENIGLKPYFKNHVYLSYDLELSKPDPVFFEAVLHDVVKQHPELLKNSTLEELKTDCWHVGDEENNDLNCPASAGWNAVLLDRMNKYNHFSESFERAQRSLHQLYTDKIDNDSGKSWQLSMKQVDAIQLSERRFVISNFRTLSQLLFPSTSNGPSSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5DI62</accession>
    <name>C5DI62_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0E09988p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0E09988g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553910.1">
        <property type="nucleotide sequence ID" value="XM_002553864.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DI62"/>
    <dbReference type="FunCoup" id="C5DI62">
        <property type="interactions" value="133"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DI62"/>
    <dbReference type="GeneID" id="8292074"/>
    <dbReference type="KEGG" id="lth:KLTH0E09988g"/>
    <dbReference type="eggNOG" id="ENOG502QQE8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_698596_0_0_1"/>
    <dbReference type="InParanoid" id="C5DI62"/>
    <dbReference type="OMA" id="LFFRYWY"/>
    <dbReference type="OrthoDB" id="25002at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="358"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="384" mass="44611" checksum="53B847B6011DB47A" modified="2009-07-28" version="1">MAQVGAKSPATTADGFNSRILWPDMIKTPDNKWVCTCKEIVDRLGTDPHVAGELKRRMEKCLMYFYVMKKQLKLFDHTYTAACILFFRYWFVFGLPPALTDCIHLSQALLVTACKTVENNRPIDVYVKGTCEFLLRDIPTSRGRQNIEKLRWELRDKLVGYEKRILCQMGFDLNLHNPKELIEEIFSGFFRYNRDFQMSEGFTKVFPKIIQDARNFIIQAGTQPVSLLCDGFTFTALSLVFCGLQYKKHVDSSFKFPKNFFTDRFPFRVTTQLFVELLTDYRVLEENFFDLKSNKGGKLQVSLEEMESLLDEDPPSDETLVSDYSTYVYEDIKDGVVREELLKHIEARVNELTERTIAQSETTEKRTIDNLSSAENPNKKQKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIK8</accession>
    <name>C5DIK8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">alcohol O-acetyltransferase</fullName>
            <ecNumber evidence="8">2.3.1.84</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Alcohol O-acetyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0E13310g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Displays enzymatic activity both for medium-chain fatty acid (MCFA) ethyl ester synthesis and hydrolysis (esterase activity). MCFA are toxic for yeast and this enzyme could thus be involved in their detoxification by esterification.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>an aliphatic alcohol + acetyl-CoA = an acetyl ester + CoA</text>
            <dbReference type="Rhea" id="RHEA:17229"/>
            <dbReference type="ChEBI" id="CHEBI:2571"/>
            <dbReference type="ChEBI" id="CHEBI:47622"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.1.84"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily. AB hydrolase 4 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.84" evidence="8"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554056.1">
        <property type="nucleotide sequence ID" value="XM_002554010.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIK8"/>
    <dbReference type="FunCoup" id="C5DIK8">
        <property type="interactions" value="350"/>
    </dbReference>
    <dbReference type="GeneID" id="8292225"/>
    <dbReference type="KEGG" id="lth:KLTH0E13310g"/>
    <dbReference type="eggNOG" id="KOG1838">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032487_1_0_1"/>
    <dbReference type="InParanoid" id="C5DIK8"/>
    <dbReference type="OMA" id="HTMDIRE"/>
    <dbReference type="OrthoDB" id="5954035at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008126">
        <property type="term" value="F:acetylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004026">
        <property type="term" value="F:alcohol O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051792">
        <property type="term" value="P:medium-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051793">
        <property type="term" value="P:medium-chain fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000137">
        <property type="entry name" value="EEB1p Acyl-coenzymeA:ethanol O-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050960">
        <property type="entry name" value="AB_hydrolase_4_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012020">
        <property type="entry name" value="ABHD4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10794">
        <property type="entry name" value="ABHYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10794:SF44">
        <property type="entry name" value="MEDIUM-CHAIN FATTY ACID ETHYL ESTER SYNTHASE_ESTERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005211">
        <property type="entry name" value="Ab_hydro_YheT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0719">Serine esterase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="11">
        <location>
            <begin position="155"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="10">
        <location>
            <position position="238"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="10">
        <location>
            <position position="387"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="10">
        <location>
            <position position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00050620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00054277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00066969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00080774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR005211-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR23619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="444" mass="50067" checksum="7DC0A2747BE703BB" modified="2009-07-28" version="1">MPLPIFNPFHWGYHGTIEQVSNPNGTVALTLKDEKKPVQFSDFVSREIPGLKDKAKFEVNPLLFTGYLQTLYLGGADFSKSFPVYYGREIVKFSDGGICTADWVMKSWKSKYGADTSSFKTDEQATHPENWPRLHPRTRFLEESEKKDVHNSEKPLVVVLHGLAGGSHEPIIRSLTQDLSNAGDSKFDVVVLNCRGCARSKITTRKLFYAVFTSDIREFIAREKARHPSRKIYAVGFSFGATMLGHYLGEEGEKAPIEAASFLCNPWDLYQSALKMNQDWWSRNLFSKNIAQFLIRLVKVNIKELEFKEGDVMPAEPASLEHPSFCVFTSKNLRKAREFGSTAEFDNLFTAPCLGFDNAMDYYKACGSIHQLPNIKVPSLIINSKDDPVVGEDSIPYKCAKESDNLVLCVSDLGGHLAFLDKKYNSWATSKIAAFFDKFEELVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIR3</accession>
    <name>C5DIR3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GTP cyclohydrolase II</fullName>
            <ecNumber evidence="3">3.5.4.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0E14542g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>GTP + 4 H2O = 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + formate + 2 phosphate + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:23704"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58614"/>
            <dbReference type="EC" id="3.5.4.25"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; riboflavin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GTP cyclohydrolase II family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.25" evidence="3"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554111.1">
        <property type="nucleotide sequence ID" value="XM_002554065.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIR3"/>
    <dbReference type="FunCoup" id="C5DIR3">
        <property type="interactions" value="158"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIR3"/>
    <dbReference type="GeneID" id="8292281"/>
    <dbReference type="KEGG" id="lth:KLTH0E14542g"/>
    <dbReference type="eggNOG" id="KOG1284">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020273_2_4_1"/>
    <dbReference type="InParanoid" id="C5DIR3"/>
    <dbReference type="OMA" id="RLPNVEC"/>
    <dbReference type="OrthoDB" id="5569761at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003935">
        <property type="term" value="F:GTP cyclohydrolase II activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00641">
        <property type="entry name" value="GTP_cyclohydro2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10990">
        <property type="entry name" value="GTP cyclohydrolase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032677">
        <property type="entry name" value="GTP_cyclohydro_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000926">
        <property type="entry name" value="RibA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036144">
        <property type="entry name" value="RibA-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001591">
        <property type="entry name" value="PRK00393.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00505">
        <property type="entry name" value="ribA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21327">
        <property type="entry name" value="GTP CYCLOHYDROLASE II-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21327:SF29">
        <property type="entry name" value="GTP CYCLOHYDROLASE-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00925">
        <property type="entry name" value="GTP_cyclohydro2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142695">
        <property type="entry name" value="RibA-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0686">Riboflavin biosynthesis</keyword>
    <feature type="domain" description="GTP cyclohydrolase II" evidence="9">
        <location>
            <begin position="109"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR23674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="306" mass="34292" checksum="4BEB6ADD73766F3A" modified="2009-07-28" version="1">MDTQGSLPIVQCVARARIPTTTGPDIFLHLYANDRDNKEHLAIVFGEDIRSRSLFKRRKNETQQERMVRGAYVGKLFPGRTVADVDENLGLELQFDNDGELRVEEKTTWAKKDTLVRIHSECYTGETAWSARCDCGEQFDRAGKLIALDREGDIVGGSGRGVIVYLRQEGRGIGLGEKLKAYNLQDLGADTVQANLMLQHPADGRDFSLGRAILIDLGIERVRLLTNNPDKVSQVEHPPQLHCVERVPMIPLSWTSDEDGIHSSEIEGYLRTKIERMGHLLQKPLKLHTMENVPASSTTITTHTII</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIS5</accession>
    <name>C5DIS5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0E14806p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0E14806g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the acetyltransferase family. MAK3 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554123.1">
        <property type="nucleotide sequence ID" value="XM_002554077.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIS5"/>
    <dbReference type="FunCoup" id="C5DIS5">
        <property type="interactions" value="482"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIS5"/>
    <dbReference type="GeneID" id="8292293"/>
    <dbReference type="KEGG" id="lth:KLTH0E14806g"/>
    <dbReference type="eggNOG" id="KOG3139">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_0_3_1"/>
    <dbReference type="InParanoid" id="C5DIS5"/>
    <dbReference type="OMA" id="LCIFARH"/>
    <dbReference type="OrthoDB" id="249099at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031417">
        <property type="term" value="C:NatC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004596">
        <property type="term" value="F:protein-N-terminal amino-acid acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000091">
        <property type="entry name" value="Peptide alpha-N-acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044542">
        <property type="entry name" value="NAA30-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45896">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45896:SF1">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="4">
        <location>
            <begin position="3"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR23686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="174" mass="20355" checksum="D4DEE440AB770BCA" modified="2009-07-28" version="1">MGIRYQPLNISNANELKHIQRLIDADLSEPYSIYVYRYFLNQWPELCFLAFSNDTENPVGCVVCKYETHRGARLRGYIGMLAVDQKYRRRGIAKKLVEQAIQKMQEIGCDEIMLETEVENTTALQLYEGMGFIRLKRMFRYYLNQGDAFKLILPLTERSCTRTTFLLQPETAFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIV4</accession>
    <name>C5DIV4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phospholipase</fullName>
            <ecNumber evidence="7">3.1.4.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0E15466g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1,2-diacyl-sn-glycero-3-phosphate + choline + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14445"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="3.1.4.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the phospholipase D family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.4" evidence="7"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554152.1">
        <property type="nucleotide sequence ID" value="XM_002554106.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DIV4">
        <property type="interactions" value="160"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIV4"/>
    <dbReference type="GeneID" id="8292323"/>
    <dbReference type="KEGG" id="lth:KLTH0E15466g"/>
    <dbReference type="eggNOG" id="KOG1329">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000690_3_0_1"/>
    <dbReference type="InParanoid" id="C5DIV4"/>
    <dbReference type="OMA" id="DSLWTKH"/>
    <dbReference type="OrthoDB" id="14911at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004630">
        <property type="term" value="F:phospholipase D activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009395">
        <property type="term" value="P:phospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01254">
        <property type="entry name" value="PH_PLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09138">
        <property type="entry name" value="PLDc_vPLD1_2_yPLD_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09141">
        <property type="entry name" value="PLDc_vPLD1_2_yPLD_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.870.10:FF:000011">
        <property type="entry name" value="Phospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025202">
        <property type="entry name" value="PLD-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016555">
        <property type="entry name" value="PLipase_D_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015679">
        <property type="entry name" value="PLipase_D_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18896:SF76">
        <property type="entry name" value="PHOSPHOLIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18896">
        <property type="entry name" value="PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00614">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13091">
        <property type="entry name" value="PLDc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009376">
        <property type="entry name" value="Phospholipase_D_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00155">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 7" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PLD phosphodiesterase" evidence="9">
        <location>
            <begin position="677"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="9">
        <location>
            <begin position="977"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="125"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="314"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1134"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1441"/>
            <end position="1479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="39"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="53"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="67"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="326"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1149"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1441"/>
            <end position="1451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1458"/>
            <end position="1473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR009376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR23715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1547" mass="178896" checksum="E00AFACF24AE26A3" modified="2009-07-28" version="1">MAPHTEQFHDAQDHTADYSVQGGVVPGISEARELHEPEAQSDPEQPEEDFDEQQQRAHDAEAHFKNLSFQGVPNSTMNGHRTPASGEEKDEQATWLKRMNRQDSFSGWRKEVGQAFRRFSKLTSRKNGRGEHARNARRSIAQAPDAEDEYTQNLASDLIDTLISGCPAALFASCSFLRDEHGVRRAPLLLAMLGVKVSPSASGETLDPQLVRQSSHTSATSVSNARHDIQNYDVKFKLELEYGVGYNRMKWTVIKSYKDLASLHSKLKLMSFQQNAINKLYIDHHRFQQLNLPHFPRFYDHSRKEGSAALDQVLEDDEASNSPEESQLREAPRRGNESLASGRTSNHFEIKKFRMKHLQDLLADQDDTNKPIHVRIERYLKLLNLALCLRPQANRLFQFYELSPIGNLLSYENGYQGKQGYMVVRSTASTQGWRVSHFRFRDWRAMVERHTNKWFLVRHSYILYVSDLHSATPLDVFLVDADFKIRVPGELEVSVDDYQDFNPLEDNSKKLSTKLMITLENSERKMQMIVKSEFQLKQWIASMSKMVKSTIWSQKKRFDSFAPIRRNCFCKFLVDGRDYFWALSEALSMAQDVIYIHDWWLSPELYMRRPFKGNQEYRIDRILKERAEKGVKIFVVVYRNVGSTVGTDSSWTKHSMLSLHPNIHLIRSPNQWLQNTYFWAHHEKMTVIDHSIAFMGGIDLCYGRYDTPEHVLRDDSPELQEQIFPGKDYSNARVCDFYDLDKPFESMYDRKEIPRMPWHDVHTMIVGEPARDMSRHFVQRWNYLLRQKRPSRPTPLLTPPSDFTEEEMKRSPLFQSLADRSTCEVQVLRSAGNWSLGLKKTEHSIQNAYLKLIETSNHYVYMENQFFVTSSSWDGVVVENKIGDALVDRIIKANSEGKTWKAFIVIPLMPGFDSPIDKPEGSSVRVIMQCQYQSISRGETSIFARLKKLDINPLQYIQFFSLRKWSTLGPNDRLVTEQLYVHGKVLIVDDRACIIGSANVNERSMLGNRDSEVAALIRDTDLVETRMGDQPYLAGRFAWELRQRLMREHLGCDVDIVEVVERKFGKLEQLAELNYKTLHTLDGDDASDREAKLASALIEVGYREVMNTESSEAWKQKHKFTVGSNGIDNPLETYNEKDEFSGPVDTENTDSKTELPQRKEEENLASCLNHYHSFNNRAGEANLGIQEKKPISTDSRLVNNEMHADDVKGLGRDGWKKVSKKFKQNVTEKLRAWADKVLSTGLRGNRSRGYDTFLPDREDVESYLQDSSVTDIEKWDMLKRICYLQHLSYKVERKKRTESDNKYSSSSMHSVSSVEGFANESARTHEKHAGILQKQELDDEIIDDMLLQLLPPIGGYKSRQKAFLKLKFIDPYAFEDPLSDLFYEDMWFAIALRNTMLFRLVFHCQPDNSVQTWRDYKDFNRMQEEFNSTQDTLIELEQQNIPGKPQPQVSNTEDDSSFKTAPNSTNAKNNASQHDSRARSSALKMKLSSSVLYGANQRIFDKHTARRILERIHGHLVVFPTDWLAKEVESKNWFYNADRLPPIDIYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJB5</accession>
    <name>C5DJB5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 9">Ubiquitin-activating enzyme E1-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">KLTH0F15070g</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1 9">Protein modification; protein sumoylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Heterodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the ubiquitin-activating E1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554841.1">
        <property type="nucleotide sequence ID" value="XM_002554795.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJB5"/>
    <dbReference type="FunCoup" id="C5DJB5">
        <property type="interactions" value="2072"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJB5"/>
    <dbReference type="GeneID" id="8293070"/>
    <dbReference type="KEGG" id="lth:KLTH0F15070g"/>
    <dbReference type="eggNOG" id="KOG2013">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013325_7_3_1"/>
    <dbReference type="InParanoid" id="C5DJB5"/>
    <dbReference type="OMA" id="PGKTECF"/>
    <dbReference type="OrthoDB" id="10255449at2759"/>
    <dbReference type="UniPathway" id="UPA00886"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031510">
        <property type="term" value="C:SUMO activating enzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019948">
        <property type="term" value="F:SUMO activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.80:FF:000004">
        <property type="entry name" value="Ubiquitin-activating enzyme E1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.520">
        <property type="entry name" value="Ubiquitin activating enzymes (Uba3). Chain: B, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.80">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, inactive adenylation domain, subdomain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.20">
        <property type="entry name" value="Ubiquitin-like 2 activating enzyme e1b. Chain: B, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042449">
        <property type="entry name" value="Ub-E1_IAD_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023318">
        <property type="entry name" value="Ub_act_enz_dom_a_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030661">
        <property type="entry name" value="Uba2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019572">
        <property type="entry name" value="UBA_E1_SCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033127">
        <property type="entry name" value="UBQ-activ_enz_E1_Cys_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF5">
        <property type="entry name" value="SUMO-ACTIVATING ENZYME SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10585">
        <property type="entry name" value="UBA_E1_SCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039133">
        <property type="entry name" value="SUMO_E1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00865">
        <property type="entry name" value="UBIQUITIN_ACTIVAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 9 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="6 9 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="THIF-type NAD/FAD binding fold" evidence="15">
        <location>
            <begin position="7"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-activating enzyme SCCH" evidence="16">
        <location>
            <begin position="297"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="545"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="587"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="10 13">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="27"/>
            <end position="32"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="59"/>
            <end position="62"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="120"/>
            <end position="125"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="427"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="430"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00073512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR039133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR039133-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR039133-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR039133-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF10585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAR24404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="598" mass="66791" checksum="159AB861D0539EB1" modified="2009-07-28" version="1">MANASAVRLLGQENYERLRSSRVLLVGAGGIGCELLKNLVLMGFGEIHIVDLDIIDLSNLNRQFLFRQRDIKQAKATTAARAIEHVSNSKLVAHQANIMDVNQFPLAWFSQFSIFFNALDNLEARRYVNQMAQYLRKPLLESGTAGFDGYIQPIIPGATECFDCTTKETPKTFPVCTIRSTPSQPIHCIVWAKNFLFSQLFASSGSMSADEDLGTDNVEEIERIRQETNELHELQELIRSGDKTRIRDVFEKVFVKDIEKLLAIEELWKAREKPTPLYNFKFDEKINKNLNTVWTIQEQVNAFVLATEKLMQRLSSEKQIEFDKDDPDTLLFVAAAANIRASVFKLPLKSVFDIKQIAGGIIPAIATTNAIIAGLSSLASLRVLNLLKNQPKANPTELNMAFTAKASNMSNNRYLSNPQLGPPNPRCPVCSIARGVLTLSQNDLDTMRLSELVGAVQEKYSYSGEVSVLDKSTQRLLADFDFEDLLNKTLAEVKVARGTILLISDEQDSEESIRAPVELYIEQGEPEGINLPDIEVPLIKPAVVEPDETNSEEGVSMPGEIILDEDDKNPKKRHLDDASETPAKKALKTEEEHVVILD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2023-06-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJE2</accession>
    <name>C5DJE2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0F15708p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0F15708g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554868.1">
        <property type="nucleotide sequence ID" value="XM_002554822.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJE2"/>
    <dbReference type="GeneID" id="8293099"/>
    <dbReference type="KEGG" id="lth:KLTH0F15708g"/>
    <dbReference type="HOGENOM" id="CLU_2558683_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJE2"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR24431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="82" mass="9414" checksum="ECC8BA2D06E87816" modified="2009-07-28" version="1">MCARNKNTNKGTFYTQEQSVWAPDLIQLLCLELDFRLKVSQADEKSFPFSNGAYSLAYGDTSTRAKLRETGIWKQNRSLLFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJS8</accession>
    <name>C5DJS8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 15">tRNA:m(4)X modification enzyme TRM13</fullName>
            <ecNumber evidence="3 15">2.1.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">KLTH0F18832g</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 15">tRNA methylase which 2'-O-methylates cytidine(4) in tRNA(Pro) and tRNA(Gly)(GCC), and adenosine(4) in tRNA(His).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>adenosine(4) in tRNA(His) + S-adenosyl-L-methionine = 2'-O-methyladenosine(4) in tRNA(His) + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43196"/>
            <dbReference type="Rhea" id="RHEA-COMP:10401"/>
            <dbReference type="Rhea" id="RHEA-COMP:10402"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74477"/>
            <dbReference type="EC" id="2.1.1.225"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 15">
            <text>cytidine(4) in tRNA(Gly)(GCC) + S-adenosyl-L-methionine = 2'-O-methylcytidine(4) in tRNA(Gly)(GCC) + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43192"/>
            <dbReference type="Rhea" id="RHEA-COMP:10399"/>
            <dbReference type="Rhea" id="RHEA-COMP:10400"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74495"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.225"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 15">
            <text>cytidine(4) in tRNA(Pro) + S-adenosyl-L-methionine = 2'-O-methylcytidine(4) in tRNA(Pro) + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32767"/>
            <dbReference type="Rhea" id="RHEA-COMP:10397"/>
            <dbReference type="Rhea" id="RHEA-COMP:10398"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74495"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.225"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the methyltransferase TRM13 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.225" evidence="3 15"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555004.1">
        <property type="nucleotide sequence ID" value="XM_002554958.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJS8"/>
    <dbReference type="FunCoup" id="C5DJS8">
        <property type="interactions" value="500"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJS8"/>
    <dbReference type="GeneID" id="8293240"/>
    <dbReference type="KEGG" id="lth:KLTH0F18832g"/>
    <dbReference type="eggNOG" id="KOG2811">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027610_1_0_1"/>
    <dbReference type="InParanoid" id="C5DJS8"/>
    <dbReference type="OMA" id="HRCSWRS"/>
    <dbReference type="OrthoDB" id="258806at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106050">
        <property type="term" value="F:tRNA 2'-O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007871">
        <property type="entry name" value="Methyltransferase_TRM13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039044">
        <property type="entry name" value="Trm13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022776">
        <property type="entry name" value="TRM13/UPF0224_CHHC_Znf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021721">
        <property type="entry name" value="Znf_CCCH-type_TRM13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12998">
        <property type="entry name" value="TRNA:M(4)X MODIFICATION ENZYME TRM13 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12998:SF0">
        <property type="entry name" value="TRNA:M(4)X MODIFICATION ENZYME TRM13 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05206">
        <property type="entry name" value="TRM13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11722">
        <property type="entry name" value="zf-TRM13_CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05253">
        <property type="entry name" value="zf-U11-48K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51800">
        <property type="entry name" value="ZF_CHHC_U11_48K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 15" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 15" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 15" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="11 15" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CHHC U11-48K-type" evidence="17">
        <location>
            <begin position="102"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="155"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="9"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU367103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAR24567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="548" mass="59283" checksum="8C3EEF82505467BB" modified="2009-07-28" version="1">MGGLPWPPERNRPEMTDREAGEAKEARDASAAAERRARPVKRACPAGTAAVPAAAQRLQCEFYLEKKQRRCGMSRRPGVAYCSEHLNQLQGAAQPPSAKGRRVPCPLDPRHTVWEKDVARHTRKCNTTKQAARPRAAEEPWFRADYNADPERGAAEVGAAGAADPADLAAAEPAAADPGNANANAAADPDDQAAVLAAIPVLRRYAAAGLAAALPLVAKHNAAVESRRFPQLQGSRKHACQQSSLIQHMLDAGLFPRDARDPLTFLEFGCGRAELSRYVNQSVFAHQTALDPARAPEPPRFVLVDRASNRMKFDKKFRDDLDELYAADRPAQQPAYAHVLRKKIDIKDLVVDRLLEARAAGADADAAADVAADVDAAAAGADAGDCVAISKHLCGVATDLTLRCCVNSDALHRRARRLRGMVIAMCCRHVCDAKQYVNRAYVEPLAQESGLAYAAFFRALKKIATWAVCGRRAGVADAEVGGHFTELSVAERERLGECARRVIDEGRARFLRERGYRVSLVRYVEPAVSLENVAMVVVRESAGAGEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJT0</accession>
    <name>C5DJT0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0F18876p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0F18876g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the BI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555006.1">
        <property type="nucleotide sequence ID" value="XM_002554960.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJT0"/>
    <dbReference type="FunCoup" id="C5DJT0">
        <property type="interactions" value="645"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJT0"/>
    <dbReference type="GeneID" id="8293242"/>
    <dbReference type="KEGG" id="lth:KLTH0F18876g"/>
    <dbReference type="eggNOG" id="KOG2322">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058671_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJT0"/>
    <dbReference type="OMA" id="FGVMSLY"/>
    <dbReference type="OrthoDB" id="7933078at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006214">
        <property type="entry name" value="Bax_inhibitor_1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23291">
        <property type="entry name" value="BAX INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23291:SF50">
        <property type="entry name" value="PROTEIN LIFEGUARD 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01027">
        <property type="entry name" value="Bax1-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="197"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="230"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="260"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="294"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="14"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR24569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="318" mass="35281" checksum="53DEC27E780C32BA" modified="2009-07-28" version="1">MKDSADTSLPAPPAYTDAAAPSKGTGPSAATAAAASPPPASPSYYQTDETRDFIPDDFKFSTTVVSCEPVVRRWFMRKVYTLLSCQILFSFAFSAWVSHTPGVQAFILRKQWLFFLAMFGGLVSCVWLTLAPRAEDVAQEPLLTPTGDGASADPARAPARAPWYCLSYRGQLALLGFFTFCEAYTLSLVTVAYNSQLILSAMLITGVVVVAVTAMALSDRFESTVTHSSSIYYWLNIALWLLIGVGFSSLFFGMNSTIDLIYSWLGATVFTIYLFIDTQMIFRKVYPDEEIRCAMMLYLDIINLFLYILRILGRNRDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5DK94</accession>
    <name>C5DK94_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">KLTH0F02794p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0F02794g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Endomembrane system</location>
            <topology evidence="5">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554332.1">
        <property type="nucleotide sequence ID" value="XM_002554286.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DK94"/>
    <dbReference type="FunCoup" id="C5DK94">
        <property type="interactions" value="20"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DK94"/>
    <dbReference type="GeneID" id="8293302"/>
    <dbReference type="KEGG" id="lth:KLTH0F02794g"/>
    <dbReference type="eggNOG" id="ENOG502QU87">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001720_0_0_1"/>
    <dbReference type="InParanoid" id="C5DK94"/>
    <dbReference type="OMA" id="TKVEWLW"/>
    <dbReference type="OrthoDB" id="10070851at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd13280">
        <property type="entry name" value="PH_SIP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042067">
        <property type="entry name" value="Sip3_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031968">
        <property type="entry name" value="VASt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14248">
        <property type="entry name" value="CYCLIN Y, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16746">
        <property type="entry name" value="BAR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16016">
        <property type="entry name" value="VASt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51778">
        <property type="entry name" value="VAST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1039"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1071"/>
            <end position="1095"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="9">
        <location>
            <begin position="309"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="VASt" evidence="10">
        <location>
            <begin position="783"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="556"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="250"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="571"/>
            <end position="580"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR23895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1232" mass="140892" checksum="09220A6B48B49F36" modified="2009-07-28" version="1">MAVEKTPKGPSHLLKLISVSFKEASLDSPSFRASTNFYHVQIESFESWIENVLRFYKHKFLPSLDDFKQISDTFFAQALPPADYLYNGVVENQMYTPMILEAFHSDLCSLSNRLLVLLRGDPESFLSHVLKISTDIIEPYREKRKNFDYYQSKHDTLLAKYQSTKISGPNVEPSSMREDAFQLFEARKSYLEASLDLAWEISQIQEALDVAISGVVGTLVRRDPMKNTIMNTPKGGDQAMDLYSRHSRWHLQMVENNASLLEDMKRAKEQVQAFTIKQLEPSRDLRDYNIKGINQASLLDHSSQGSKKSTEKSGWLFMKTQVGKPARTVWVRRWCFVKKSVFGMFLLSPAETAVEETDKFGVLLMNVRYDPNEERKFCFEAQIAGAEPGTNNGRSNTAERISLVFQTESLSDLKSWLQVFDEAKKHVLSLEKDDSEYELSFSRIPPIFFEFACSSSNQTDQQLTSFDPNGLYGKSILQLMKAAFSETGSIIFPQDSPFETPLLKTPISTKLTKLSVISSYYLVNDGVPNAITANFWGSVNWSEFCFNTRKVDGNNYKERQSPVPEAHTPQAERTSPTWSPMNYPDFYQRGMRNADLQFRSLFFPTAIQRNLERSDELLLLSFPCSWSPNTKQGFSGMCYITARNIYFYMNSMGFICLLNRSLEDIVSVEEVKIEGDNKAGILKLYDMNELSMSCSIFFDNPELIATKIRQLLQNGTTEELKSEKEILSLFASIDRDFDDKSRPSTPSTNLKGGEEATAVESLFRPLENPLNELRKRQQSFQSRYCSVFKMDFNISPKGLMHVLFGDKSSAFPSCYLLADRSGSGNTTTAWQVRDSGDLKEAVRIVHFKIGHTQTFFPPEGNGSGDSKNVAYLDMLQRMKLAKEGEYYEVDQESGHIKVPFAKAFRVLSKYIIISRSDPNPRKSKGLDKASSCSLYLYYDVAFSDEMTGARFRNLNVFDKFLKTLVLHTLRYESVLTRNVVQQYLDKIGKHGRVLKAIRLCGQLALVQGSEKTAHLGDNGKTNVARYSRSSLLRFMMRTILFYTVNVIFAVTRMFFQFIYAVLTNARMINKAVLLALALSIIANFSLLGKTTWSYWSVKAADKLFKSYVDIENKPMERALYIDDLDLLSDSLTNVQDACLEKFLESTREVESKYRQTRHELALKRNELLVELKILNTMEKEMVHGEYVTFLLNEMQNCRTVEKDMPDVWANDSQLHDHCSRCSSEYKSIQHLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKD6</accession>
    <name>C5DKD6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cell division control protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0F03784g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the CDC6/cdc18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554374.1">
        <property type="nucleotide sequence ID" value="XM_002554328.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKD6"/>
    <dbReference type="FunCoup" id="C5DKD6">
        <property type="interactions" value="1192"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKD6"/>
    <dbReference type="GeneID" id="8292568"/>
    <dbReference type="KEGG" id="lth:KLTH0F03784g"/>
    <dbReference type="eggNOG" id="KOG2227">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012774_2_1_1"/>
    <dbReference type="InParanoid" id="C5DKD6"/>
    <dbReference type="OMA" id="WPTDEVY"/>
    <dbReference type="OrthoDB" id="1926878at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033314">
        <property type="term" value="P:mitotic DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049945">
        <property type="entry name" value="AAA_22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016314">
        <property type="entry name" value="Cdc6/18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015163">
        <property type="entry name" value="Cdc6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054425">
        <property type="entry name" value="Cdc6_ORC1-like_ATPase_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050311">
        <property type="entry name" value="ORC1/CDC6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763:SF26">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13401">
        <property type="entry name" value="AAA_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22606">
        <property type="entry name" value="Cdc6-ORC-like_ATPase_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09079">
        <property type="entry name" value="WHD_Cdc6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001767">
        <property type="entry name" value="Cdc6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01074">
        <property type="entry name" value="Cdc6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="8">
        <location>
            <begin position="114"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="Cdc6 C-terminal" evidence="9">
        <location>
            <begin position="402"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR001767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR23937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="499" mass="55840" checksum="993078B60580D53F" modified="2009-07-28" version="1">MQTQMLESVVLGSRKRLAFEVCEDPVTVKRFQGLVTPPGSPVKKCKTKEVSTLGSPIRLHFGASSVYSRTKAALQRCADVSQQGTVNLGACLPTRGEQYDRIMAFLNSVVGSGHGDSLYITGPPGTGKTAQLDLVVREKFQTLVLEAPQLQQDYALRNTSFFTTKSGHHESVAIVSINCIAVNSAESIFAKIYHSCSGDSARTKVRNADDLQKFMQAHPQTTFIVILDEMDKLVTSTLQDANATKHIFDLFLMAKLPHLKFVLIGIANSLDMKDRFLSRLNLKHELMPQTVTFTPYTAEQMFEIVMQKLKSLDFDPIIQPIAIRFAAKKCSGNTGDLRKVFDILRSSIEVLELDSIKNRHTLNETTAKVTLAHVAKVFSTLHNNFSTKSRISKLNMQQRLVLCSLVHRERSDVFQTQTSLDEAYDYYSKLLNRKDSISPLKRDEFFEICNALETCGVVNIVQGKSSGKTKHIVKFIKANVDKKEFEDEISKLASLKTYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKF7</accession>
    <name>C5DKF7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">YL17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0F04312g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="4">Component of the large ribosomal subunit (LSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes). uL22 is associated with the polypeptide exit tunnel.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uL22 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554395.1">
        <property type="nucleotide sequence ID" value="XM_002554349.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKF7"/>
    <dbReference type="FunCoup" id="C5DKF7">
        <property type="interactions" value="1144"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKF7"/>
    <dbReference type="GeneID" id="8292590"/>
    <dbReference type="KEGG" id="lth:KLTH0F04312g"/>
    <dbReference type="eggNOG" id="KOG3353">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083987_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKF7"/>
    <dbReference type="OMA" id="NTYETAR"/>
    <dbReference type="OrthoDB" id="10254664at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000448">
        <property type="term" value="P:cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00336">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.470.10:FF:000010">
        <property type="entry name" value="60S ribosomal protein L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.10">
        <property type="entry name" value="Ribosomal protein L22/L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001063">
        <property type="entry name" value="Ribosomal_uL22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018260">
        <property type="entry name" value="Ribosomal_uL22_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005721">
        <property type="entry name" value="Ribosomal_uL22_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036394">
        <property type="entry name" value="Ribosomal_uL22_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01038">
        <property type="entry name" value="uL22_arch_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11593">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11593:SF10">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00237">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54843">
        <property type="entry name" value="Ribosomal protein L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00464">
        <property type="entry name" value="RIBOSOMAL_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="161"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00062111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00082773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR23958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="184" mass="20678" checksum="DEBAC547721C99C4" modified="2009-07-28" version="1">MARYGATSTNPAKSAAARGSYLRVSFKNTRETAQAINGWGLERAQKYLDQVLDHQRAIPFRRFNSSIGRTGQGKEFGVTKARWPAKSVKFVQDLLQNAAANAEAKGLDSTKLFVSHIQVNKAPKQRRRTFRAHGRINKYESSPSHIELVLTEKEEAVEKAEEKKVVRLSSRQRGRLAAQKRITA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKU7</accession>
    <name>C5DKU7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sulfhydryl oxidase</fullName>
            <ecNumber evidence="6">1.8.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0F07656g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2</text>
            <dbReference type="Rhea" id="RHEA:17357"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:16520"/>
            <dbReference type="ChEBI" id="CHEBI:17412"/>
            <dbReference type="EC" id="1.8.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="1.8.3.2" evidence="6"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554535.1">
        <property type="nucleotide sequence ID" value="XM_002554489.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKU7"/>
    <dbReference type="FunCoup" id="C5DKU7">
        <property type="interactions" value="31"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKU7"/>
    <dbReference type="GeneID" id="8292740"/>
    <dbReference type="KEGG" id="lth:KLTH0F07656g"/>
    <dbReference type="eggNOG" id="KOG3355">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070631_2_2_1"/>
    <dbReference type="InParanoid" id="C5DKU7"/>
    <dbReference type="OMA" id="MIDKEWR"/>
    <dbReference type="OrthoDB" id="59470at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016971">
        <property type="term" value="F:flavin-dependent sulfhydryl oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.310:FF:000002">
        <property type="entry name" value="Sulfhydryl oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.310">
        <property type="entry name" value="ERV/ALR sulfhydryl oxidase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039799">
        <property type="entry name" value="ALR/ERV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036774">
        <property type="entry name" value="ERV/ALR_sulphydryl_oxid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017905">
        <property type="entry name" value="ERV/ALR_sulphydryl_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12645">
        <property type="entry name" value="ALR/ERV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12645:SF1">
        <property type="entry name" value="FAD-LINKED SULFHYDRYL OXIDASE ERV2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04777">
        <property type="entry name" value="Evr1_Alr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69000">
        <property type="entry name" value="FAD-dependent thiol oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51324">
        <property type="entry name" value="ERV_ALR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 6" id="KW-0274">FAD</keyword>
    <keyword evidence="2 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950416" description="Sulfhydryl oxidase" evidence="7">
        <location>
            <begin position="32"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="ERV/ALR sulfhydryl oxidase" evidence="8">
        <location>
            <begin position="68"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU371123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR24098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="201" mass="23007" checksum="25A6328A5BDFFB47" modified="2009-07-28" version="1">MIHRQRLVSILAAVSLLSLWFFFSSNKLSYSTPNDPVVDLDTNTNTEHTDPQGQNKQAKLKFQSIMPSMPDQKAKQELGRASWKYFHTVLSRFPDEPTQEERDKLAQFLQLYAELYPCGECSYHFVEMLKKWPPQTSSRTAAALWGCLMHNKVNVFLKKDEYDCSKILEDYDCGCGGEDGKINDQLKMNKVSLQKEGKQGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKZ8</accession>
    <name>C5DKZ8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0F08800p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0F08800g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554586.1">
        <property type="nucleotide sequence ID" value="XM_002554540.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKZ8"/>
    <dbReference type="FunCoup" id="C5DKZ8">
        <property type="interactions" value="568"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKZ8"/>
    <dbReference type="GeneID" id="8292792"/>
    <dbReference type="KEGG" id="lth:KLTH0F08800g"/>
    <dbReference type="eggNOG" id="KOG3662">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011607_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKZ8"/>
    <dbReference type="OMA" id="LHCMKYP"/>
    <dbReference type="OrthoDB" id="5977743at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000093">
        <property type="entry name" value="Cell division cycle-related protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033308">
        <property type="entry name" value="PGAP5/Cdc1/Ted1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13315">
        <property type="entry name" value="METALLO PHOSPHOESTERASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13315:SF4">
        <property type="entry name" value="METALLOPHOSPHOESTERASE, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="381"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="455"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="7">
        <location>
            <begin position="124"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR24149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="477" mass="55669" checksum="33BF86809DFC84F0" modified="2009-07-28" version="1">MPYRRRDKESAAPFSSAGGERNKVLRPKEKNGSSIWGKLCWKYLISFLCLWVCLIQYYERRVIKRAVNRCQWSKWENWPSGASPHRMALLADPQIMDAHSYPSRPWIVNYFTQQLLDNYHARNWKHLHYHLDPDSTFFLGDLFDGGRRWNDSDWFKEYERFNKIFPKKPNRLTVMSLPGNHDIGFGDTVIEDSLTRFKFYFGDPSSAWEVGNHTIVLLDTISLSDTKNENVSAVPRAFLDSFASIPKKHPRIMLTHVPLYRDPISQTCGSKRESQKPFPMMKGVQYQTVIDHELSQEVLQTISPSLVFSGDDHDYCHISHTYLLPIGQQTAEEITVKSCAMNMGISKPAIQLVSLYNPDDTFASETYHTDICYLPDPYKPIKVYVFTYILTTILFFWMHFHPSSFNRLVGSTYDRKTEDLANVLPIANKKSDSRSSRQELDELFSTHKKLMFERFALNMALMTLMIFLVFVFYYKQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>C5DL21</accession>
    <name>C5DL21_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Autophagy-related protein 20</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0F09328g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Preautophagosomal structure membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the sorting nexin family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554609.1">
        <property type="nucleotide sequence ID" value="XM_002554563.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DL21"/>
    <dbReference type="FunCoup" id="C5DL21">
        <property type="interactions" value="167"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DL21"/>
    <dbReference type="GeneID" id="8292816"/>
    <dbReference type="KEGG" id="lth:KLTH0F09328g"/>
    <dbReference type="eggNOG" id="KOG2273">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014456_2_1_1"/>
    <dbReference type="InParanoid" id="C5DL21"/>
    <dbReference type="OMA" id="ICNTDIT"/>
    <dbReference type="OrthoDB" id="289314at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06867">
        <property type="entry name" value="PX_SNX41_42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044106">
        <property type="entry name" value="PX_Snx41/Atg20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051079">
        <property type="entry name" value="Sorting_Nexin_Autophagy"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46979:SF1">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46979">
        <property type="entry name" value="SORTING NEXIN-41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0072">Autophagy</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0967">Endosome</keyword>
    <keyword evidence="8" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PX" evidence="13">
        <location>
            <begin position="141"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="22"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="369"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="22"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="42"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="73"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="109"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="125"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR24172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="616" mass="69663" checksum="CBCACB7DBC466518" modified="2009-07-28" version="1">MGDSSESKPDSVGDSSILVDIKRTLENDEDKPSNGADGQELTAKNANLDLSNTTHAGLMRTEILEQDNPFTNKRPEIASDTSSLKENRLLENPNKQYQSRTDVDEDTEPLNTQPRSHSSGSGSMDIKRPEPSTREADTGLGDKVYISEASKASEGQGRTYIAYTIKYKASTVKRRYSDFESLRKILIKLFPMTLIPPIPEKQSITSYGKSMAGSKSSYLLPSEAAGSVDLSLSVINGSVNTNDEKLIRHRIRMLTSFLNRLLKNEEITKTSIITDFLDPNNTNWNDVITSSATISSLPKSVLLCNPLDPTNTTRAHIFLPIPSSSTQLLLNKEILATTAHDEADDGFGKIEQEYRNYEHVMEAGFYKYNKRITKNFHALRQEMKELSEAFAEFSNDQKRGAELAEQLSHLSNAFDESALELESIVGKLYYNINEPLSEAVHMAGAAQELLRYRRLKSVQKTILIRTLLYRKAQVKKLQERESEARKVDHMVDEQVGQQDHINLKHPQNRTYSGKFMTKINKIASMVKETVSYQDPDPTTAIENLEKEISQLSESLEMSEKDLGIISTTIKDYELPKFSKERNEEVSDIMKNYTKYLKSFAERNLKLWQDVKQQQGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DL40</accession>
    <name>C5DL40_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Non-structural maintenance of chromosomes element 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0F09746g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the SMC5-SMC6 complex, that promotes sister chromatid alignment after DNA damage and facilitates double-stranded DNA breaks (DSBs) repair via homologous recombination between sister chromatids.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the SMC5-SMC6 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the NSE4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554628.1">
        <property type="nucleotide sequence ID" value="XM_002554582.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DL40"/>
    <dbReference type="FunCoup" id="C5DL40">
        <property type="interactions" value="32"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DL40"/>
    <dbReference type="GeneID" id="8292835"/>
    <dbReference type="KEGG" id="lth:KLTH0F09746g"/>
    <dbReference type="eggNOG" id="KOG2866">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041037_5_1_1"/>
    <dbReference type="InParanoid" id="C5DL40"/>
    <dbReference type="OMA" id="IFQMDMP"/>
    <dbReference type="OrthoDB" id="361242at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030915">
        <property type="term" value="C:Smc5-Smc6 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027786">
        <property type="entry name" value="Nse4/EID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014854">
        <property type="entry name" value="Nse4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029225">
        <property type="entry name" value="Nse4_Nse3-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16140">
        <property type="entry name" value="NON-STRUCTURAL MAINTENANCE OF CHROMOSOMES ELEMENT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16140:SF0">
        <property type="entry name" value="NON-STRUCTURAL MAINTENANCE OF CHROMOSOMES ELEMENT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15412">
        <property type="entry name" value="Nse4-Nse3_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08743">
        <property type="entry name" value="Nse4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4 7" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="5 7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nse4/EID protein Nse3/MAGE-binding" evidence="10">
        <location>
            <begin position="97"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="Non-structural maintenance of chromosome element 4 C-terminal" evidence="9">
        <location>
            <begin position="287"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="152"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF15412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR24191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="383" mass="44046" checksum="9BBC8FAB34929A7C" modified="2009-07-28" version="1">MTRQREEDDERGNLSSKRRRTEVPQAEAEQASALEEDLEFEILQGYRDVDKDVSKDRVEVARNGDIAIALDRLKAIDTLFDRMVSVNSNRNSLLAQDSKAALNVSELAQLSVRNLKLDESQRVLGISDVINYSKRFMLKEYFNANGIKQERAKVNSSDDAEEEDSSNAQDDSATNQDVYAQGRQQRSLLDQFDQYNRFTQFNWFKMGMLFRTISKTPLTVDHLLGPFAAEKKIRSSSQRIRTTDIVGEAVTAERVTKSSLNSTQEETTPEQVKKCFKVLIEKNGLKQINLFKFIIHPKSFAKSVENLFYTSFLIKGGKLVLEEDEDGFPAIRPKEGLPKNPHDREVEIQRRNDARQNHIIFQLDMATWRRLIQRFDITSPFIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>C5DL49</accession>
    <name>C5DL49_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">DNA polymerase</fullName>
            <ecNumber evidence="21">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="ordered locus">KLTH0F09966g</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="20">Catalytic component of DNA polymerase delta (DNA polymerase III) which participates in chromosomal DNA replication. Required during synthesis of the lagging DNA strands at the replication fork, binds at/or near replication origins and moves along DNA with the replication fork. Participates in leading strand synthesis during replication initiation and termination. Has 3'-5' proofreading exonuclease activity that corrects errors arising during DNA replication.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 21">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 21">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 21">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="21"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554637.1">
        <property type="nucleotide sequence ID" value="XM_002554591.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DL49"/>
    <dbReference type="FunCoup" id="C5DL49">
        <property type="interactions" value="816"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DL49"/>
    <dbReference type="GeneID" id="8292845"/>
    <dbReference type="KEGG" id="lth:KLTH0F09966g"/>
    <dbReference type="eggNOG" id="KOG0969">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000203_2_0_1"/>
    <dbReference type="InParanoid" id="C5DL49"/>
    <dbReference type="OMA" id="CNNCRPR"/>
    <dbReference type="OrthoDB" id="2414538at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043625">
        <property type="term" value="C:delta DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008296">
        <property type="term" value="F:3'-5'-DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006287">
        <property type="term" value="P:base-excision repair, gap-filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045004">
        <property type="term" value="P:DNA replication proofreading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006297">
        <property type="term" value="P:nucleotide-excision repair, DNA gap filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05777">
        <property type="entry name" value="DNA_polB_delta_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05533">
        <property type="entry name" value="POLBc_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.690:FF:000001">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.730:FF:000004">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.730:FF:000006">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000004">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.730">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.60">
        <property type="entry name" value="DNA polymerase family B, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.690">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017964">
        <property type="entry name" value="DNA-dir_DNA_pol_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006133">
        <property type="entry name" value="DNA-dir_DNA_pol_B_exonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006134">
        <property type="entry name" value="DNA-dir_DNA_pol_B_multi_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042087">
        <property type="entry name" value="DNA_pol_B_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050240">
        <property type="entry name" value="DNA_pol_type-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025687">
        <property type="entry name" value="Znf-C4pol"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00592">
        <property type="entry name" value="pol2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10322">
        <property type="entry name" value="DNA POLYMERASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10322:SF23">
        <property type="entry name" value="DNA POLYMERASE DELTA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00136">
        <property type="entry name" value="DNA_pol_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03104">
        <property type="entry name" value="DNA_pol_B_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14260">
        <property type="entry name" value="zf-C4pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00106">
        <property type="entry name" value="DNAPOLB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00116">
        <property type="entry name" value="DNA_POLYMERASE_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 21" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 21" id="KW-0235">DNA replication</keyword>
    <keyword evidence="17 21" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="14 21" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="13" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15 21" id="KW-0408">Iron</keyword>
    <keyword evidence="16 21" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 21" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6 21" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="18 21" id="KW-0539">Nucleus</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 21" id="KW-0808">Transferase</keyword>
    <keyword evidence="12 21" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 21" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DNA-directed DNA polymerase family B exonuclease" evidence="24">
        <location>
            <begin position="247"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B multifunctional" evidence="23">
        <location>
            <begin position="544"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="domain" description="C4-type zinc-finger of DNA polymerase delta" evidence="25">
        <location>
            <begin position="1007"/>
            <end position="1078"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="8"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="29"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="62"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00055225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU000442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF00136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF03104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF14260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EMBL" id="CAR24200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1095" mass="123581" checksum="0D90D52540BCD0CB" modified="2009-07-28" version="1">MGTQSLKRPGEGEPKDPESVERRMKPQSFDHSSLSEPVSTIEIIPSDKFKKQKTVGYKPKDSTNASQTLTPTQFEEQLSSMDHDTVNAQEHGQEKTWSRESLPSDFSSQTHSISFQQLDAEQSFIPGYVDQNTSAVVRFFGVTDQGHSVLCSVTGFRHYLYVPAPAGFTQQDIHGFLEYLNSSYDNTVDEIEITARQSIWGYSGTAKLPFLKIYTNNPNAINKLRTGFERGHINYNDSWFSGGTTSYDNIAYPLRLMIDCGITGMSWITLPEGKYSMTPPNQKVSTCQLEVRINFKDLIARPAEGEWSHSAPLRILSFDIECSGRVGVFPEPDVDPVIQIANVVSVAGSSKSFIRNVFTVNSCSPITGSQIFEHKTENEMLKHWRQFVVETDPDVIIGYNTANFDLPYLIDRARALKIEEFPYFGRLSNVKQEVKESVFSSKAYGTRTSKNINIDGRLQLDLFQFVQREYKLRSYTLNAVSAHFLGEQKEDVHYSIISSLQNGDSETRRRLAVYCLKDAFLPLRLMEKLMALVNYTEMARVTGVPFSYLLSRGQQIKVVSQLFRKCLQIDTVIPNMQSQGSDEQYEGATVIEPIRGYYDLPIATLDFNSLYPSIMMAHNLCYTTLASKTTVETLKLKKDEEYIITPNGDYFVTSKVRSGILPEILYELINARKRAKNDLKAEKDPFKRDVLNGRQLALKISANSVYGFTGATVGKLPCLAISSSVTSFGRTMIEKTKEAVEEKYSLKNGFKHDAVVVYGDTDSVMVKFGTHDLAEAMELGTEAAAYVSSLFKNPINLEFEKAYFPYLLINKKRYAGLYWTNPNKYDKLDQKGLASVRRDSCPLVSIVMNKVLKKILIDRDVDGALKFIKEIIEEILHNRADISKLIISKTLAPNYTNPQPHAVLTERMKKRDGTGPNVGDRVDYVVIGGNDKLYNRAEDPLYVLEHNIQIDSKYYLTNQLQNPIISIIAPIIGEKQANSMFVVKSIKISTGNAKGGLMGFIKKVQTCKGCKGPLKKGEGPLCKNCSSRSGELYVRALYDVRDLEEKFARLWTQCQRCSGSLHNEVLCSNKNCDIFYMRVKAKKELQEKVEELGKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DL94</accession>
    <name>C5DL94_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0F11066p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0F11066g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="7">Interacts with G-actin; ADP-actin form.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the actin-binding proteins ADF family. Twinfilin subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554682.1">
        <property type="nucleotide sequence ID" value="XM_002554636.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DL94"/>
    <dbReference type="FunCoup" id="C5DL94">
        <property type="interactions" value="416"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DL94"/>
    <dbReference type="GeneID" id="8292895"/>
    <dbReference type="KEGG" id="lth:KLTH0F11066g"/>
    <dbReference type="eggNOG" id="KOG1747">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031995_0_2_1"/>
    <dbReference type="InParanoid" id="C5DL94"/>
    <dbReference type="OMA" id="YLFKHTH"/>
    <dbReference type="OrthoDB" id="10006997at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003785">
        <property type="term" value="F:actin monomer binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030042">
        <property type="term" value="P:actin filament depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051016">
        <property type="term" value="P:barbed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11284">
        <property type="entry name" value="ADF_Twf-C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11285">
        <property type="entry name" value="ADF_Twf-N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002108">
        <property type="entry name" value="ADF-H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028458">
        <property type="entry name" value="Twinfilin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13759">
        <property type="entry name" value="TWINFILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13759:SF1">
        <property type="entry name" value="TWINFILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00241">
        <property type="entry name" value="Cofilin_ADF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00102">
        <property type="entry name" value="ADF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51263">
        <property type="entry name" value="ADF_H"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ADF-H" evidence="9">
        <location>
            <begin position="5"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="ADF-H" evidence="9">
        <location>
            <begin position="167"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="290"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="312"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR24245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="321" mass="35239" checksum="FFAF7A79545CC428" modified="2009-07-28" version="1">MSNQSGITADESLLGSFGSLANNESPSDVVVAEISSDNTTVKLCTTLSNAAELEIFAKREERPLYVFIRDSGRIVFISYVPESAPVRSKMLYASTKNTVLRQVGSNHISKQLLASLPEELSPGFWSSDASDEPAPLTEAEQISARISSEQRIESARGGRQLVSQTGGTSHTLSFKIASGEPIPSLLEQHNFVSFKINLEKEQVEVLSTAQLPGAREVPRSLSQNHPTYNIYKNNGKLHFIYSCPSGSKVKERMLYASNKSGFVKHLKEMDHLTMETVLEIGDSDELEISMLEKSSPATSEHGNPAPGQLKFNRPKRPGRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLJ6</accession>
    <name>C5DLJ6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Dolichyl-diphosphooligosaccharide-protein glycosyltransferase subunit OST5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0G01232g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the oligosaccharyltransferase (OST) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the OST5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555094.1">
        <property type="nucleotide sequence ID" value="XM_002555048.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLJ6"/>
    <dbReference type="FunCoup" id="C5DLJ6">
        <property type="interactions" value="126"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLJ6"/>
    <dbReference type="GeneID" id="8293352"/>
    <dbReference type="KEGG" id="lth:KLTH0G01232g"/>
    <dbReference type="eggNOG" id="ENOG502SD2H">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_183917_0_0_1"/>
    <dbReference type="InParanoid" id="C5DLJ6"/>
    <dbReference type="OMA" id="CATINIM"/>
    <dbReference type="OrthoDB" id="4047914at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008250">
        <property type="term" value="C:oligosaccharyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007915">
        <property type="entry name" value="TMEM258/Ost5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05251">
        <property type="entry name" value="Ost5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR24657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="89" mass="9528" checksum="60CB091417F52512" modified="2009-07-28" version="1">MSSYQEVYKLYHQASEFEGVVALESQPMYGTITAVLALVFIALALSSISKAAGLPLGLQFVQYSFLSLAGSAFFGLATIFLTNSFGVYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLW5</accession>
    <name>C5DLW5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">KLTH0G04070p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0G04070g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555213.1">
        <property type="nucleotide sequence ID" value="XM_002555167.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLW5"/>
    <dbReference type="FunCoup" id="C5DLW5">
        <property type="interactions" value="1176"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLW5"/>
    <dbReference type="GeneID" id="8293479"/>
    <dbReference type="KEGG" id="lth:KLTH0G04070g"/>
    <dbReference type="eggNOG" id="KOG1691">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066963_3_0_1"/>
    <dbReference type="InParanoid" id="C5DLW5"/>
    <dbReference type="OMA" id="DVFEACF"/>
    <dbReference type="OrthoDB" id="759142at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015720">
        <property type="entry name" value="Emp24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
        <property type="entry name" value="TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950537" evidence="9">
        <location>
            <begin position="19"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="10">
        <location>
            <begin position="31"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR24776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="209" mass="23697" checksum="2CD13B283C0DA39A" modified="2009-07-28" version="1">MQFSILPLLCVLFSTCLALHFDITASSNPEPVCVRDFINEGQLVVVRIETDGSVGDGQVLNLVIRDSNGNEPRRSKDIAGTTNVAFHAARSGAFDICFENHLQVHGRSLKRSVELDVETGSQARDWNKIQSSEKLKPVEVELRRIEELTDEIVDELGYLKAREERLRDTNESTNRRVRNFSLMVICVLVSLGAWQINYLHNYFKAKHII</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLZ1</accession>
    <name>C5DLZ1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL4m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0G04642g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555239.1">
        <property type="nucleotide sequence ID" value="XM_002555193.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLZ1"/>
    <dbReference type="FunCoup" id="C5DLZ1">
        <property type="interactions" value="375"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLZ1"/>
    <dbReference type="GeneID" id="8293505"/>
    <dbReference type="KEGG" id="lth:KLTH0G04642g"/>
    <dbReference type="eggNOG" id="KOG1624">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041575_4_2_1"/>
    <dbReference type="InParanoid" id="C5DLZ1"/>
    <dbReference type="OMA" id="KTFGPHP"/>
    <dbReference type="OrthoDB" id="275876at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002136">
        <property type="entry name" value="Ribosomal_uL4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013005">
        <property type="entry name" value="Ribosomal_uL4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023574">
        <property type="entry name" value="Ribosomal_uL4_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10746">
        <property type="entry name" value="50S RIBOSOMAL PROTEIN L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10746:SF6">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL4M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00573">
        <property type="entry name" value="Ribosomal_L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52166">
        <property type="entry name" value="Ribosomal protein L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="67"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR24802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="254" mass="28409" checksum="DA2011A82A8CA096" modified="2009-07-28" version="1">MRGIRMQSSQSKALSGAALPPAFTLATLRSFPSLEPLTFVPVPASVLDAPLRRDILWKAVVYENDNKRVGSSNPPGRSENGYSRRKLLPQKGSGKARAGDANSPTRHNGARALARSAPNDYTTKLPQKVYSQAFINALSYQYRRGNLFIIGGTHSLCETHDLDVNELEVLPTSLENDDGELVFEKFLQEHELQKQNLLFVINAPRGGLFRYSEPFKENVNIVQKEFVDVNDILKARRIFIELEALEYLAVTHSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DM01</accession>
    <name>C5DM01_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA repair and recombination protein RAD52</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0G04862g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in DNA double-strand break (DSB) repair and recombination. Promotes the annealing of complementary single-stranded DNA and by stimulation of the RAD51 recombinase.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RAD52 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555249.1">
        <property type="nucleotide sequence ID" value="XM_002555203.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DM01"/>
    <dbReference type="FunCoup" id="C5DM01">
        <property type="interactions" value="324"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DM01"/>
    <dbReference type="GeneID" id="8293515"/>
    <dbReference type="KEGG" id="lth:KLTH0G04862g"/>
    <dbReference type="eggNOG" id="KOG4141">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011431_3_2_1"/>
    <dbReference type="InParanoid" id="C5DM01"/>
    <dbReference type="OMA" id="MFSDDFQ"/>
    <dbReference type="OrthoDB" id="206565at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000730">
        <property type="term" value="P:DNA recombinase assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045002">
        <property type="term" value="P:double-strand break repair via single-strand annealing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006312">
        <property type="term" value="P:mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.80:FF:000001">
        <property type="entry name" value="DNA repair protein RAD52 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.80">
        <property type="entry name" value="DNA repair protein Rad52/59/22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004585">
        <property type="entry name" value="DNA_recomb/repair_Rad52"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041247">
        <property type="entry name" value="Rad52_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007232">
        <property type="entry name" value="Rad52_Rad59_Rad22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042525">
        <property type="entry name" value="Rad52_Rad59_Rad22_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00607">
        <property type="entry name" value="rad52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12132">
        <property type="entry name" value="DNA REPAIR AND RECOMBINATION PROTEIN RAD52, RAD59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12132:SF1">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD52 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04098">
        <property type="entry name" value="Rad52_Rad22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="196"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="386"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="432"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="432"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="466"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00041062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR24812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="479" mass="51818" checksum="0F4082F52A2B53F4" modified="2009-07-28" version="1">MIEKSLIHVGIKSARPIGVSQAAMDASKTNGSSTEDIQAKLDKKLGPEYISKRVGFGANRVAYIEGWKAINLANQIFGYNGWSTEVKSITIDFMDERQGKFCIGCTAIIRVTLSDGTFREDIGYGTVENERRKSSAFERAKKSAVTDALKRSLRAFGNALGNCLYDKSFLAKIDKVKFDPPDFDEGNLYRATDEFAENSRAGDSSRGAPTGLPVGTPRSDHEARGPPYKRRNINQTPNVVPLPAPARELQPQHAQQPQPPSDQDPDELLDDSFMFSDELQDDDIIAAGEAQPTTPALDRPVESHPNPQAQVQTPVAFVTAKAAPNLQNKAPVPPGSVFDPKFRAQSIRHTVDQTTSAHVKTSVLRERGLPNNRSAIYNQYAPKGKLIGDTNTAQDSSVPPRERQPILPAVASTATTTNITHHEASAKEVQNNGYANAGQPNGTSVHAGNVIPPAVSSPAATVRSVGKPKAHHPNVRNPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>C5DM02</accession>
    <name>C5DM02_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.26</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">HECT-type E3 ubiquitin transferase TOM1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0G04884g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the UPL family. TOM1/PTR1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="4"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555250.1">
        <property type="nucleotide sequence ID" value="XM_002555204.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DM02">
        <property type="interactions" value="1179"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DM02"/>
    <dbReference type="GeneID" id="8293516"/>
    <dbReference type="KEGG" id="lth:KLTH0G04884g"/>
    <dbReference type="eggNOG" id="KOG0939">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000215_0_1_1"/>
    <dbReference type="InParanoid" id="C5DM02"/>
    <dbReference type="OMA" id="DCHFSRE"/>
    <dbReference type="OrthoDB" id="8068875at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000004">
        <property type="entry name" value="E3 ubiquitin-protein ligase HUWE1, variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase NEDD4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1750.10:FF:000003">
        <property type="entry name" value="E3 ubiquitin-protein ligase UPL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010309">
        <property type="entry name" value="E3_Ub_ligase_DUF908"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010314">
        <property type="entry name" value="E3_Ub_ligase_DUF913"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050409">
        <property type="entry name" value="E3_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025527">
        <property type="entry name" value="HUWE1/Rev1_UBM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254:SF67">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HUWE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254">
        <property type="entry name" value="HECT DOMAIN UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06012">
        <property type="entry name" value="DUF908"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06025">
        <property type="entry name" value="DUF913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14377">
        <property type="entry name" value="UBM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <keyword evidence="7 12" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="HECT" evidence="14">
        <location>
            <begin position="2918"/>
            <end position="3254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1881"/>
            <end position="1903"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1947"/>
            <end position="2016"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="2050"/>
            <end position="2103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="2288"/>
            <end position="2319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="2412"/>
            <end position="2438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="1882"/>
            <end position="1893"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="2054"/>
            <end position="2073"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="2093"/>
            <end position="2103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="2288"/>
            <end position="2301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="2302"/>
            <end position="2316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="2412"/>
            <end position="2431"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="12">
        <location>
            <position position="3221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00076267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR24813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="3254" mass="370631" checksum="6CF69CABFD26F78B" modified="2009-07-28" version="1">MVTLTKLEKFRKEEKAKGFKPLLDDLTQCSEAEFVKKLTNIITWDRSRDDLYIWIPVLNRIDDILNRLLAHYNYKSTNRKTQPVKLIIMSAEDETVTKELLWFTCRLLNNTCNRSLYSSLDVMTNLLNCPNYVVKLGAMKVAAIVGEQQVIARQKIDSKNLLSNKELKRKTLDLALALPSSTTDDRMDHFALVDLFFDKKQYPTKWSKIDFSYYAAAPKVYLTQGALNKTGTGSVKRFIMNAEELSSLSLQQVFDKGMAEIPAESWFDFSLQATVAKAFSDSTYENLQLRNLIIQTKFNAIALANVIYIPAQVSSQLFEVDPYAFNNLTDFISLSEQKLPKDLRVDALFSLECISLKHIWCSDIVRNLGGNMSHGLLFQILRYIAKLLRDNNSKELDEEYNVRFFYLVSNLADVKTLQESMLAAGLIPNLLEIISIKDCAYKRTLASATHLLEVFIDDADATTEFIGNNGFNILIGCVNEEVNFALEHPEFGEPPKYPVVYYSISFRQLGYIRSLLKLVLKLLKTDSGDRIRNLIDSPILLSLNQILENRPVFGYTLVTYALDVVQTILNTEPTIYSVLVESKTIPYIIDNFKHFLGPSGELLCLLPEVISALSLNNDGLKQVKEKGLINCLFEIMTTPELAKIMSWDDSAIDLGTSIDELARHYPELKPVIEDCFYKTVKALPSIISFSHPYVYRSTISNGEFYHSRDETVVDNEEGGSEIESGEVQVPAAVLDCFSAVFYGMTLENVSWASLAEKVDIKDLLKVIIIERPPYDYVGSQTLLNFSDILKHFDDERRNYALPALLSLLSEKLDGVKDFLEYNFEKPYVLSTPYEVLDETLRKLTVINVLLYVITDIYINIASLFPIRVLQIMEFFEQNGFNLIVKLRHLFQRSALEELYMRNRLPASVTAETTMRPIGNTPPLTIYASKPSKNDPKDDKTSAKFKNTLQDRQLFIKMQSWTSMLFRCFLRLTHARKMTVEASDRALEIRIFDTTVNEIVKMMDLKYLLSHLGYFLVVFNFNTFVFTFPNVTTSVGGTVQTIPALLFYQAGGFKIYYEAIKLLFANLQKFNDVENIEKFDYIKDSEDILTASALINALSFFNKCIQMETMENIRNTKEYYPYDEVYYNLTSALIIPIKVLSLGLVDELVNEFSILEQGTRRLPYSVFKQLLTMIKNIFNSTAEGEDHDLYELRWDLMPPSHRKIEMLKSCGIEEDIAKNYLEEQKDDLPIHVKPDVFSDGEWETYKALRETGKWKTDLSLLEPQYKNFSTASDLSEMRSSFYANGFEDRILQLLPMYPKLINAISHMILQLLQEAEEPPKKVLGKLLQMMRGIDLSDASVLAPVIHLFGILLNDKAMYEKSEDFIHEFVQYLQQSLRPEFVNSAWFSKALYVYEIIFAKSEAPRLDPLPDGIEEGVNTPCPPLFKAFTISSDAKSKIFDSLIRVHEISDFYSALAISRILILYTRDEHYSHEVPQSGILSKILKTIGIFQKSEKINYLESSFLLLARRCFETSEIVSSLIQYELSRAFTTRAIGDSKENSRDLTGLVSEKANVVMRDPASFVNCLCETARFEDFVRPDKLENLIVRRYLSERDEEMSDANSQADGKLSLSNRTGIVHLLLSQLMAAYKKDWTSEPVKEESVEDTKKKDDVDVARNPVCAYMIFLLKVLIELVGSYKQSKLEFLTFNKRNMYVESPKPRTTALNFVLYQLLDTGGRDQDKHYVERKEVIGKLARDLVVSFVSSVQDKEVQKQDPKIVNPDMTYIRKFTIEAVTKALKSVAKSPKTLEANVSKLYGWLHLVTSFLVSERSYLHSVLDSNKTYVDKYQIGKLMLDMNVPSTIADCMASLDLNYPFSKKLFNKSVEPLNAINEIRSRFSDLFKLENTDEDDEVEDESEKEEHSDMFRNSALGMYDVEDIEEDDDDDASLIGEDEDIAFVEGEDGEIEVVFSEDERGHHHGGSSSSDGMNDSDQIDDSEGSMDYGLDGESLDMDIASGESSNSDIGNGGDGIESDDSSYYTDHEGPLEYVHIDDGAPAPVIDVELDSSELAESDWESRLSDLSGSDWESGLSELSGSEDGSSEENFNDETHPGSRWLADEVDMDDDSDEEGRGVFTGIQHVFSPEPQYFRVHGENHRGHRGPHHHSSRRADFMMATPMSLSTGSRGTRGILMNPLGPSGLEEVENGILNQLTSTGSSSRPRNELPHLSDILFSGEFFDEKSSVGIVLKSTTARWSDIFDMFYDSKIYANNVIPTIIGRIFQPSSDLYVRKREEALEKEKAEKLQREENLRKQSEFKKRKLEEMHTDGSDEGENEEEEDEEEGQEHLPPIYVNIGGEEVNIAGTDIDPEFLDALPDDMRAEVFAQHIRERRAEALDQTVDSREIDADFLESIPENLREEILEQEAAESRFSRLMGSLNDEENAESSGDVGEENDADESPDSKKKKPEKIYFSPLVDRSGISAIMRSLFISQPYLAREVYHELFYRLCSSKQNRSDIMNILMLILTEGINDHQSLERIYNLISNRALGIAKSTSARMLPLDCTPLSVANQTIEVLQNLLETDNRLKFFFITEHENLLINKTPLKNKKDIFTKNMKWPINYLFSLLDKKIITDETVLLDLLTRILQIITKAISAIAKNENSQKKFTVPDIETKHLEAVVSIVKLDACNTKVFQQTLNIMTHLSVIKGAQDIFTKELCTLAIQTVEVLKTDLSNLDSEIGEVEIGTEINYETFQKFTVPSSEQAKLLKVLTAIDYIHTNKKKAGDIDVEKLMGLYDQIKLGGVWTSLGHCLEKFDEKPALSSSANILLPLIESLMVVCKHSKTRDSRDVLKYQAKKSDFSEVAAENIFFAFTDLHKKLLNQMIRSNPKLMSGPFALLVKNPKILDFDNKRYYFIAKLRSGAQEKPKLSISVRREQVFLDSYRSLFFKSNEDIKNSKLEITFKGEAGVDAGGITREWYQVLSRQMFNPDYALFLPVASDKTTFHPNRTSGINPEHLSFFKFIGMIIGKAICDQCFLDCHFSREVYKNILSKPVALKDMESLDLDYYKSLIWILENDITDIIEETFSVETDDYGEHKVIDLIEDGHNVPVTEQNKQEYVKKIVEYKLHTSVKEQMDNFLLGFYAIIPKDLVSIFDEQELELLISGLPDIDVDDWKNNTNYVNYTPTCKQISYFWRAVRSFDSEERAKLLQFVTGTSKVPLSGFKELTGVNGISKFSIHRDYGSTERLPSSHTCFNQLDLPAYDSYETLRGSLLLAINEGHEGFGIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>C5DM14</accession>
    <name>C5DM14_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">sterol 14alpha-demethylase</fullName>
            <ecNumber evidence="17">1.14.14.154</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Cytochrome P450 51</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Cytochrome P450-14DM</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Cytochrome P450-LIA1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Sterol 14-alpha demethylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="33" type="ordered locus">KLTH0G05148g</name>
    </gene>
    <organism evidence="33 34">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="33 34" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="34">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>32-hydroxylanosterol + reduced [NADPH--hemoprotein reductase] + O2 = 32-oxolanosterol + oxidized [NADPH--hemoprotein reductase] + 2 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:75107"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:166681"/>
            <dbReference type="ChEBI" id="CHEBI:166806"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:75108"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>32-oxolanosterol + reduced [NADPH--hemoprotein reductase] + O2 = 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + oxidized [NADPH--hemoprotein reductase] + H2O + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:75111"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:17813"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:166681"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:75112"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>a 14alpha-formyl steroid + reduced [NADPH--hemoprotein reductase] + O2 = a Delta(14) steroid + formate + oxidized [NADPH--hemoprotein reductase] + H2O + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:68068"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:138031"/>
            <dbReference type="ChEBI" id="CHEBI:176902"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="29">
            <dbReference type="Rhea" id="RHEA:68069"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>a 14alpha-hydroxymethyl steroid + reduced [NADPH--hemoprotein reductase] + O2 = a 14alpha-formyl steroid + oxidized [NADPH--hemoprotein reductase] + 2 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:68064"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:176901"/>
            <dbReference type="ChEBI" id="CHEBI:176902"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:68065"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>a 14alpha-methyl steroid + 3 reduced [NADPH--hemoprotein reductase] + 3 O2 = a Delta(14) steroid + formate + 3 oxidized [NADPH--hemoprotein reductase] + 4 H2O + 4 H(+)</text>
            <dbReference type="Rhea" id="RHEA:54028"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:138029"/>
            <dbReference type="ChEBI" id="CHEBI:138031"/>
            <dbReference type="EC" id="1.14.14.154"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:54029"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>a 14alpha-methyl steroid + reduced [NADPH--hemoprotein reductase] + O2 = a 14alpha-hydroxymethyl steroid + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:68060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:138029"/>
            <dbReference type="ChEBI" id="CHEBI:176901"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:68061"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>lanosterol + 3 reduced [NADPH--hemoprotein reductase] + 3 O2 = 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + 3 oxidized [NADPH--hemoprotein reductase] + 4 H2O + 4 H(+)</text>
            <dbReference type="Rhea" id="RHEA:25286"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:16521"/>
            <dbReference type="ChEBI" id="CHEBI:17813"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="1.14.14.154"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:25287"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>lanosterol + reduced [NADPH--hemoprotein reductase] + O2 = 32-hydroxylanosterol + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:75103"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16521"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:166806"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:75104"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 30">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="16">Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 1/6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 31">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.154" evidence="17"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555262.1">
        <property type="nucleotide sequence ID" value="XM_002555216.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DM14"/>
    <dbReference type="FunCoup" id="C5DM14">
        <property type="interactions" value="624"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DM14"/>
    <dbReference type="GeneID" id="8293528"/>
    <dbReference type="KEGG" id="lth:KLTH0G05148g"/>
    <dbReference type="eggNOG" id="KOG0684">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_15_0_1"/>
    <dbReference type="InParanoid" id="C5DM14"/>
    <dbReference type="OMA" id="HWFPFVG"/>
    <dbReference type="OrthoDB" id="1055148at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008398">
        <property type="term" value="F:sterol 14-demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11042">
        <property type="entry name" value="CYP51-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000033">
        <property type="entry name" value="14-alpha sterol demethylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050529">
        <property type="entry name" value="CYP450_sterol_14alpha_dmase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24304:SF2">
        <property type="entry name" value="24-HYDROXYCHOLESTEROL 7-ALPHA-HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24304">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 30 31" id="KW-0349">Heme</keyword>
    <keyword evidence="9 30 31" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13 32" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 30 31" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 31" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="8 31" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="34" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="15" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="10" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="14" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="32" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="32" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="32">
        <location>
            <begin position="26"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="32">
        <location>
            <begin position="61"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="30">
        <location>
            <position position="475"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00037887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00038974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00042513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00042983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00043106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00043156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00047587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00047670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00047702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00048479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00048866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00049163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00049450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="PIRSR" id="PIRSR602403-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="EMBL" id="CAR24825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="536" mass="61441" checksum="8962A408E279180C" modified="2009-07-28" version="1">MESGSSSVVEKFVGKGIEAAKVALTWFLALPIAQQASLLIAAPFLYVITWQILYSMRRDRVPLVFYWIPWVGSAVSYGMRPYEFFAECQKKYGDVFSFMLLGRVMTVYLGPKGHEFVLNAKLADVSAEAAYTHLTTPVFGKGVIYDCPNSRLMEQKKFVKGALSTDAFRSYVPMISEEILQYFRDSKNFEMNKHNSGAINVMETQPEMTIFTASRSLLGKEMRQMLNTDFAYLYSDLDKGFKPINFVFSHLPLEHYRKRDHAQRTISGTYMKLIQKRKQNNDIKDRDLIDSLLKNSTYKDGTPMTDQEIANLLIGVLMGGQHTSAATSAWLLLHLAERPDLQEELYKEQMEVLNNGKDELTYEALQKMPLLNQTIKETLRMHHPLHSIFRRVERNLSVPNTPYVIPRGHFVLVSPGYCHLQDEYFPKANTFDPHRWDNDYQSSYATGEEVDYGFGKISKGVSSPYLPFGGGRHRCIGEHFAYCQLGVILSTYIRTVKWKFTPNMHGVPKPDFQSMVTLPLCPAEVEWEKRNPQQTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMA5</accession>
    <name>C5DMA5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0G07238p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0G07238g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Mitochondrial ribosome (mitoribosome) assembly factor. Binds at the interface of the head and body domains of the mitochondrial small ribosomal subunit (mt-SSU), occluding the mRNA channel and preventing compaction of the head domain towards the body. Probable inactive methyltransferase: retains the characteristic folding and ability to bind S-adenosyl-L-methionine, but it probably lost its methyltransferase activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555353.1">
        <property type="nucleotide sequence ID" value="XM_002555307.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMA5"/>
    <dbReference type="FunCoup" id="C5DMA5">
        <property type="interactions" value="336"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMA5"/>
    <dbReference type="GeneID" id="8293622"/>
    <dbReference type="KEGG" id="lth:KLTH0G07238g"/>
    <dbReference type="eggNOG" id="KOG2539">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024759_0_0_1"/>
    <dbReference type="InParanoid" id="C5DMA5"/>
    <dbReference type="OMA" id="HRKCPLQ"/>
    <dbReference type="OrthoDB" id="421327at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052571">
        <property type="entry name" value="Mt_RNA_Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015324">
        <property type="entry name" value="Ribosomal_Rsm22-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016522">
        <property type="entry name" value="RSM22_mit_bud"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13184">
        <property type="entry name" value="37S RIBOSOMAL PROTEIN S22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13184:SF5">
        <property type="entry name" value="METHYLTRANSFERASE-LIKE PROTEIN 17, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09243">
        <property type="entry name" value="Rsm22"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007797">
        <property type="entry name" value="RSM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="423"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00045681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR24916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="707" mass="79715" checksum="1FC607CD2165A7AB" modified="2009-07-28" version="1">MSLRLALVQSCRAHISYSSMVRFRLAFPTSYTRILKRFKGTGASFSRQDLDVFTMDKQGGGTRGAAGFVVEDNQLGNEEVVDRDELLRNEYVSSFRKGQGTLPPGTNSREARLLPETITGRAPRDQIELNPTVSQAIRNNILSLHMPGNLRRSAGSGFVELKQSKVHRAARTNMEVDSHIASLFLQNYGSIFQSLSELKKRLGDKFKPDRILDVGYGPATGIIALNDLMGPDFRPYSKEAVILGHIEMQKRAKILLSRQANEAPNDPNIYSDAEAAETDQIEVDNDLVGEVMTKKIKTVTKLRDSVPSSKSYDLIILTHQLLKAEEKFPIEVDMNIEWYLKMLAPGGHIILIERGNPMGFEIIARARQVMLRPENFAEEHGKIPRPWTKGISTEVFNKREVGSSHETPQKDLSTDFFERAPEDEEVSGAKKVQESSQPGEPSGESYFLKVVAPCPHHKKCPLQIGKPQYYDYGEGRDLKFCNFQKSVLRPKYAIELKKGRVLATPWQNNAEAVGIKGMAKPGTGRPHGRNFEILSYSYLIMERSPADDETVQRINMERSQQQDKYGVGSLGDNTPSTWPRIIRQPLKRKGHVVMDMCGASGQLEKWTVPRSFSKEIYHDARKAMKGDLWGLEAKTKTRGMGDLNVGRLEKVEGEKIREAKRQMKRKDREMKQAVDNVDYGDPLSYEETVDTMAAMYGSEFEKSHKKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMD9</accession>
    <name>C5DMD9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribose-5-phosphate isomerase</fullName>
            <ecNumber evidence="4">5.3.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">D-ribose-5-phosphate ketol-isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Phosphoriboisomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0G08052g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>aldehydo-D-ribose 5-phosphate = D-ribulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:14657"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="EC" id="5.3.1.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate degradation; pentose phosphate pathway; D-ribose 5-phosphate from D-ribulose 5-phosphate (non-oxidative stage): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ribose 5-phosphate isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.6" evidence="4"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555387.1">
        <property type="nucleotide sequence ID" value="XM_002555341.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMD9"/>
    <dbReference type="FunCoup" id="C5DMD9">
        <property type="interactions" value="963"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMD9"/>
    <dbReference type="GeneID" id="8293658"/>
    <dbReference type="KEGG" id="lth:KLTH0G08052g"/>
    <dbReference type="eggNOG" id="KOG3075">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056590_0_2_1"/>
    <dbReference type="InParanoid" id="C5DMD9"/>
    <dbReference type="OMA" id="ACHVQEK"/>
    <dbReference type="OrthoDB" id="1555531at2759"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00412"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004751">
        <property type="term" value="F:ribose-5-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006014">
        <property type="term" value="P:D-ribose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009052">
        <property type="term" value="P:pentose-phosphate shunt, non-oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01398">
        <property type="entry name" value="RPI_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1360:FF:000014">
        <property type="entry name" value="Ribose 5-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.260:FF:000053">
        <property type="entry name" value="Ribose-5-phosphate isomerase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004788">
        <property type="entry name" value="Ribose5P_isomerase_type_A"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00021">
        <property type="entry name" value="rpiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11934">
        <property type="entry name" value="RIBOSE-5-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11934:SF0">
        <property type="entry name" value="RIBOSE-5-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06026">
        <property type="entry name" value="Rib_5-P_isom_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75445">
        <property type="entry name" value="D-ribose-5-phosphate isomerase (RpiA), lid domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR24950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="275" mass="30290" checksum="7B48B36714D4A32A" modified="2009-07-28" version="1">MFRNLKSLVHTSVRRFAAKMTSIPDVKNLPTLHSTLEEAKRVAAYRAVDENLDYANHRVIGVGSGSTVIYVAERLGQYLLDPQFSIYASQFICIPTGFQSKELILTNGLRLGSVEQYPVIDIVFDGADEVDVNLQLIKGGGGCLFQEKLVSTSASKFVVVADYRKRSPKYLGTNWVKGVPIEVVPTSYIRVLNDLKTKLKCKNAVLRQGAPAKAGPVVTDNCNFIIDAHFGEIEHPEKLHQDIKMLVGVVETGLFIDNASKAYFGNPDGSVEMLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMJ6</accession>
    <name>C5DMJ6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0G09482p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0G09482g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the actin family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555444.1">
        <property type="nucleotide sequence ID" value="XM_002555398.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMJ6"/>
    <dbReference type="FunCoup" id="C5DMJ6">
        <property type="interactions" value="788"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMJ6"/>
    <dbReference type="GeneID" id="8293722"/>
    <dbReference type="KEGG" id="lth:KLTH0G09482g"/>
    <dbReference type="eggNOG" id="KOG0681">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008246_1_0_1"/>
    <dbReference type="InParanoid" id="C5DMJ6"/>
    <dbReference type="OMA" id="YPFTEHV"/>
    <dbReference type="OrthoDB" id="7340501at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="CDD" id="cd10211">
        <property type="entry name" value="ASKHA_NBD_Arp5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000018">
        <property type="entry name" value="Chromatin remodeling complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000058">
        <property type="entry name" value="Putative actin-related protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="298"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="424"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="493"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="19"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR25007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="745" mass="85656" checksum="98764078419F26CB" modified="2009-07-28" version="1">MPRDSSLPPLKIHTLSEPDASDTPEPFDDAADYDPNVPIAIDFGSSEVRAGFGNQDQPSHIFTNRISRWRDRRKNRNLTFIGNDTNLDLSVRSQAKSPYDGAIVSNWDYTEEIMEYTFHHLGVNGNDGVSNPILMTEKLATLQSQRENWYQLLFECFGVPQVTFGIDSLFSFYANNDATASGLVINSGNEDTHVIPVMNGKGVLSEAKRINWGGRQSVEYLSNLLALKYPYFPIKPSTHQFQSLYEDFCYVSVDYQDEIRKILTLEELETKNIVVEAPFAETVQPEKSEEELRLQAEKRRETGRRLQEQAKQRRQEKLAQKEEEYEYYTQLKEQMKDQSKKAVLSTLQSAGFDDEQDFKKYLVGLEKTLKRAQVLEAGEEEEVSLADKFDLVDVPDEQLNEEQIKEKRKQRLLKANFDARVKAKEEKLKKEQEEEEARQKDEAWRSEDLQGWIKDKRQKLTALIKTRKEKLKIKNDMKDRKSHAAQKRMKNIATLAEERSRSSAKRSRQQSTVDNDPNDTFGANDEDWLVYNDITSNPEALDQAIEEEYNVIVGLEQQLLEYDPNFTEEDTLDAQYDWRNSTLHLFLRGPRPHDSENINEQHQMHLNVERIRVPEVIFQPSMAGLDQAGVTEICETNFLKKFGSTRSQLSEGSKKLAANVFLTGGNMRLPGVRERIVREFTGMLPMGSNLNVRMASDSAVDAWRGMAKLSQAGSQYKGSYITRKEYQELGADYIKEHNLGNVLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMJ8</accession>
    <name>C5DMJ8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Fatty acid synthase subunit alpha</fullName>
            <ecNumber evidence="8">2.3.1.86</ecNumber>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="8">3-oxoacyl-[acyl-carrier-protein] reductase</fullName>
                <ecNumber evidence="8">1.1.1.100</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="8">3-oxoacyl-[acyl-carrier-protein] synthase</fullName>
                <ecNumber evidence="8">2.3.1.41</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0G09526g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a (3R)-hydroxyacyl-[ACP] + NADP(+) = a 3-oxoacyl-[ACP] + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17397"/>
            <dbReference type="Rhea" id="RHEA-COMP:9916"/>
            <dbReference type="Rhea" id="RHEA-COMP:9945"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78776"/>
            <dbReference type="ChEBI" id="CHEBI:78827"/>
            <dbReference type="EC" id="1.1.1.100"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>a fatty acyl-[ACP] + malonyl-[ACP] + H(+) = a 3-oxoacyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:22836"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9916"/>
            <dbReference type="Rhea" id="RHEA-COMP:14125"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78776"/>
            <dbReference type="ChEBI" id="CHEBI:138651"/>
            <dbReference type="EC" id="2.3.1.41"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the thiolase-like superfamily. Fungal fatty acid synthetase subunit alpha family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.86" evidence="8"/>
    <dbReference type="EC" id="1.1.1.100" evidence="8"/>
    <dbReference type="EC" id="2.3.1.41" evidence="8"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555446.1">
        <property type="nucleotide sequence ID" value="XM_002555400.1"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMJ8"/>
    <dbReference type="GeneID" id="8293724"/>
    <dbReference type="KEGG" id="lth:KLTH0G09526g"/>
    <dbReference type="eggNOG" id="ENOG502SMKZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000114_0_0_1"/>
    <dbReference type="InParanoid" id="C5DMJ8"/>
    <dbReference type="OMA" id="QPILANM"/>
    <dbReference type="OrthoDB" id="4251012at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005835">
        <property type="term" value="C:fatty acid synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004316">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004312">
        <property type="term" value="F:fatty acid synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004321">
        <property type="term" value="F:fatty-acyl-CoA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008897">
        <property type="term" value="F:holo-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042759">
        <property type="term" value="P:long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00828">
        <property type="entry name" value="elong_cond_enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.25.70:FF:000001">
        <property type="entry name" value="Fatty acid synthase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040899">
        <property type="entry name" value="Fas_alpha_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047224">
        <property type="entry name" value="FAS_alpha_su_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026025">
        <property type="entry name" value="FAS_alpha_yeast"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041550">
        <property type="entry name" value="FASI_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050830">
        <property type="entry name" value="Fungal_FAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018201">
        <property type="entry name" value="Ketoacyl_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10982:SF21">
        <property type="entry name" value="FATTY ACID SYNTHASE SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10982">
        <property type="entry name" value="MALONYL COA-ACYL CARRIER PROTEIN TRANSACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18325">
        <property type="entry name" value="Fas_alpha_ACP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18314">
        <property type="entry name" value="FAS_I_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000454">
        <property type="entry name" value="FAS_yeast_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00606">
        <property type="entry name" value="KS3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52004">
        <property type="entry name" value="KS3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="6" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="2 8 10" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ketosynthase family 3 (KS3)" evidence="13">
        <location>
            <begin position="1131"/>
            <end position="1656"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="121"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="632"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="357"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="125"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="active site" description="For beta-ketoacyl synthase activity" evidence="9">
        <location>
            <position position="1315"/>
        </location>
    </feature>
    <feature type="modified residue" description="O-(pantetheine 4'-phosphoryl)serine" evidence="10">
        <location>
            <position position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000454-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000454-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS52004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR25009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1738" mass="192884" checksum="67A3C0A12CAC2BE2" modified="2009-07-28" version="1">MSPENQDELCHVLLTELLAYQFAWPVRWVETQEVLLNERQTERYIEIGPQPILANMAKRTLKQNENYENATTISTRREVFSVAGDMDKICYRYDPPAQSVEKTDAPEENAQGKINDGTEIPRAQEISTSCNSSGATSGRSFSTQPELTTELVVQSVVANKIAGYSIETLPLSHTLKELCKGKSTLQNEIKGDLYKELPGLQDAQLEDMSLESICKEVESPKTLGPVTLGEVTKFIPRKFAGKLGTVSAVKEYLSRSWGVNNSAPLLLFIASRDLKTRFSSEEETQQFIDNSCVEFAKFKKIDYSLESAKTNDVGAGCSAETDEKPKTVDIEVYNECNSDLMQLQKEQHNVMGQHLKVTDAQCEIEILKSKLEAMEGKLSAIGEEFGLDYIEKSLTRSFSPLKIRVYDSAWNWGKQSVLQLLHAALLNPQKAVEILSPNEIHNLVNRADLSIIKIIQHKLETEPFSLSVKRRFEGIIQACLRDISSQPVYSATQFTSYEPLGSEHATMQHVRSYDPSQYIKEMSVGTTKSSAETSIVSTKLDSDMSSYYKIEKELFQVYSKIIQYSLASNNSPHNIRAQFETIYEQLLIFLRNSDHVASFFRGIINEAVFSVNKSLITTKDDEQDIIVSDCEISSSDDEDDVPPQPVRQTHQPNVSIPTGVVPFLHLKRRSHVSGEWIYDKALTQMYLNNLLRIGKAGVSFSDKRALILVSDAKDNLSLEVINALLQAGTVVVVATQRYDHETTSAFQKSYQKYGASGSKLIVLPLNISSKIDVFRFSEYLFSNIGDIDFLLPMNVKATPGSILDYSSEAELTHRSSSVNLLRLLGCFVKEKRVHAMETRPTHVLLPLSPNHANIYEESNFKDTFLALVLQKWYDEDWSTELSICGCVYGWTNDDGGDLVARGLEKMGVRTFANNEMALNMLGLLSYNVVECSQESPLIADLNGGLQTLPNISSVVSKLQKEMATSVEIREEIEEQARITRELRGVKEPEVQFVEPKGNVRSDFPQMLPYHEVRAKFNGEQLSGLLDLSQVVVVTGFAEIGPWGSARTRWEMEKSGEFSLEGCIELACVMGLIEYQSSAERSGWVDCKSGLPVEEVDIKQRYEAQILEHTGIRIIEPELFQGYDPKRKQLLHEVIITHDLSPIQMDAASAQQYKLQHGELVEVYPIAETGNSECHVKFLKGCSLMIPKALRLDRFVAGQVPTGWDPARYGIGEDITSQVDQVTLYALIATAEALISSGITDPYEMYQYVHVSEVGNCSGSGIGGMNSLRAMRQERLKDTDVQNDIIQETFVNASAAWVNMLLLSSSGPIKTPVGACATALESLDNAVETIRSGQAKVCIAGGYDDLEGHVSHEFGNMGATVNSDKELQSGRDPREMCRPATSTRAGFLEAHGAGIQVLMSAEVAIEMGVPIYGIVAMTSTASDKIGRSLPAPGKGILTCARQQPRARSARSAKLDARYRKRQLQARLREIDDWAQNELAAGAPDVGPAEARLVQQLAHRQAADAKKHWGNYFWHDNPRVSPLQGALATFGLTVDDLTVASCHGTSTKANELNESQILDTIMQHLGRTPGNPLLAVFQKHLTGHPKGAAGAWMANGLLQLMQDRVVPGNRNADNIDQGFRRFEHLLYPSENILVADVKAACLTSFGFGQKGAMAVLVNANYVLAALSESEYTHYAARVRAREARAQRRLADALVHHTTCRFKDQAPFSAAHEEAVYLDPFARADDELRVRPLRDQARGPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMP1</accession>
    <name>C5DMP1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kish</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0G10472g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">Involved in the early part of the secretory pathway.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Golgi apparatus membrane</location>
            <topology evidence="2 9">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the KISH family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555489.1">
        <property type="nucleotide sequence ID" value="XM_002555443.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMP1"/>
    <dbReference type="FunCoup" id="C5DMP1">
        <property type="interactions" value="221"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMP1"/>
    <dbReference type="GeneID" id="8293767"/>
    <dbReference type="KEGG" id="lth:KLTH0G10472g"/>
    <dbReference type="eggNOG" id="KOG3808">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_152663_1_0_1"/>
    <dbReference type="InParanoid" id="C5DMP1"/>
    <dbReference type="OMA" id="ICSCTYV"/>
    <dbReference type="OrthoDB" id="10034655at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051523">
        <property type="entry name" value="KISH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009653">
        <property type="entry name" value="Ksh1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13229">
        <property type="entry name" value="PROTEIN KISH-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06842">
        <property type="entry name" value="DUF1242"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005125172" description="Protein kish" evidence="9">
        <location>
            <begin position="27"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU910717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR25052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="72" mass="8242" checksum="8ADC4CF2EB28F808" modified="2009-07-28" version="1">MSALFNFQALLQVLLLLICSCTYIHSQWPSLLDRYKEHSMLGAFWKCARIGERASPYVSIACIIMAVNQFQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMR6</accession>
    <name>C5DMR6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">holo-[acyl-carrier-protein] synthase</fullName>
            <ecNumber evidence="1">2.7.8.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0G11066g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.8.7" evidence="1"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555514.1">
        <property type="nucleotide sequence ID" value="XM_002555468.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMR6"/>
    <dbReference type="FunCoup" id="C5DMR6">
        <property type="interactions" value="43"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMR6"/>
    <dbReference type="GeneID" id="8293793"/>
    <dbReference type="KEGG" id="lth:KLTH0G11066g"/>
    <dbReference type="eggNOG" id="KOG0945">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075352_0_0_1"/>
    <dbReference type="InParanoid" id="C5DMR6"/>
    <dbReference type="OMA" id="PWAGIFV"/>
    <dbReference type="OrthoDB" id="26719at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008897">
        <property type="term" value="F:holo-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.20">
        <property type="entry name" value="4'-phosphopantetheinyl transferase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008278">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037143">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050559">
        <property type="entry name" value="P-Pant_transferase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12215:SF10">
        <property type="entry name" value="L-AMINOADIPATE-SEMIALDEHYDE DEHYDROGENASE-PHOSPHOPANTETHEINYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12215">
        <property type="entry name" value="PHOSPHOPANTETHEINE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01648">
        <property type="entry name" value="ACPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56214">
        <property type="entry name" value="4'-phosphopantetheinyl transferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="4'-phosphopantetheinyl transferase" evidence="3">
        <location>
            <begin position="176"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR25077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="320" mass="35887" checksum="086A5A96E759A7F6" modified="2009-07-28" version="1">MQSLTRKVLYGLQLSVGPFCEIPSKVLCLINKLSLGYILSIVFDQSRRTPVGFNQMSKEWLLSAGTGLPAFVLVCDDHRGILHDDFEFEMGMRYLPLEKQQRILSRKQRDARNTALCNQILQIAGASAACGIHWRDLKFKMSKLGKPYVESANPCIFNLSNSCGKVAMFVKRSHGSIGIDLASTKDCENWGPGYLRLFQDIFSSDELQCLEQTQQGLQRDRLFIYYWSLKEAYTKLTGTGLNCCLSAINLGALEPLEADKTAKIVRTINGSPITFKSKWIDNLTVLSVCEDSGNSTAEHDELPLYELEVADILSYLDSMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMS7</accession>
    <name>C5DMS7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0G11308p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0G11308g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555525.1">
        <property type="nucleotide sequence ID" value="XM_002555479.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMS7"/>
    <dbReference type="FunCoup" id="C5DMS7">
        <property type="interactions" value="58"/>
    </dbReference>
    <dbReference type="GeneID" id="8293804"/>
    <dbReference type="KEGG" id="lth:KLTH0G11308g"/>
    <dbReference type="eggNOG" id="KOG4532">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027079_0_0_1"/>
    <dbReference type="InParanoid" id="C5DMS7"/>
    <dbReference type="OMA" id="INSWARR"/>
    <dbReference type="OrthoDB" id="418169at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019417">
        <property type="entry name" value="DUF2415"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011044">
        <property type="entry name" value="Quino_amine_DH_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43991:SF9">
        <property type="entry name" value="DUF2415 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43991">
        <property type="entry name" value="WD REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_8G05640)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10313">
        <property type="entry name" value="DUF2415"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50969">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2415" evidence="2">
        <location>
            <begin position="386"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="146"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="486"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="491"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="516"/>
            <end position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR25088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="684" mass="77898" checksum="C79488CCB5D64A9A" modified="2009-07-28" version="1">MTISEDSGSLDFEDDGSRGSDKVFQNYLMPTLKLYDTRVSINHWQLRDCVKHSSTEPGKIYYIYEHSIRVLDTRPHRQEPGSRVRTKPFGERKRRNSSPKWPVNSQLHSPSQEVVHLDFKSRCFQENSGILVSGGLIGPDDAGNWGHYARHPPSRSGSESNTLNTSNRVSPMRMTSSTVLSDHSSYSNPDSWKGVVSIYNEENDACVTYRLGQYINNCVSINRRNSQQYDLFACNNDAHLYQCDISNRGVELTRRYSDLKFPLNNATLSHDGKSLITSGDSCKFAIYRQSQVTGFYSLKYDFQPEWGSSFIRSERIPRYAMPDRSGFVDHIYEVPGGDHGFYTSFSENDMMFATVFQNGTCCVYDTRKLETPLAELSSTRKHTQNGAFRVCKFSKGLDDLLFISEHHGRVHVIDTRNFMNHQVIMIPDRNDSSTEQKITAPAGRRSSLPVELNDPHVTFATSIPVNELQPKLLPYPKLVNCMDNSIENDQTENKPTEETPALRRERRRSEFRVRRYSTSNNHPTSSLESIDPSILDTRYSEQNYRAVDNYNYVGASSRGGEYSYERLLQSREDTESGILFDNEIDFAEAYSDGSDSTTQAVVQRSQQRIPNQFGPRVTQYTGGTDRTRIGSNPAVSSVEENSISGIDWMDDEEGSSLIIGTDYGIMKWNINSWARRSFPSYDFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMT2</accession>
    <name>C5DMT2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Exocyst complex component SEC5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0G11418g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the exocyst complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the SEC5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555530.1">
        <property type="nucleotide sequence ID" value="XM_002555484.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMT2"/>
    <dbReference type="FunCoup" id="C5DMT2">
        <property type="interactions" value="345"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMT2"/>
    <dbReference type="GeneID" id="8293809"/>
    <dbReference type="KEGG" id="lth:KLTH0G11418g"/>
    <dbReference type="eggNOG" id="KOG2347">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_339546_0_0_1"/>
    <dbReference type="InParanoid" id="C5DMT2"/>
    <dbReference type="OMA" id="MSAKWKS"/>
    <dbReference type="OrthoDB" id="26242at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029175">
        <property type="entry name" value="EXOC2/Sec5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039481">
        <property type="entry name" value="EXOC2/Sec5_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13043:SF1">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13043">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT SEC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15469">
        <property type="entry name" value="Sec5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex component EXOC2/Sec5 N-terminal" evidence="5">
        <location>
            <begin position="78"/>
            <end position="896"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU365069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF15469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR25093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="897" mass="103381" checksum="139F290B5443DDCE" modified="2009-07-28" version="1">MGDSAFTFGARELCDFYQLKDLDPKVSWAEDSSVVYSLSQPQNADSIDSSYVVLNELLRQEHEHSTERNDTIETAGLSDPLNSSTRLEDLLDHYRIPQDERYKYYINSKKFNSKLYLKQLHAQDSFKDLSLSLDHLDQSLQAQSEDLKQLVQRNFVKYVRSKNNLDRIYEQFNKFSLGESRDFGTDDLGETVDESIREITIKVKPILDISTKRRNAQTTIAFLQDHKQFFDAPKKLKHCLIEKDFANLVVEYNNAHSTFKDLQRRGFTFPILTKIWDDIENTICHYREVIWDSLVNLVAGETQEQILPLISKLLDLNYAGNPIIEWINTKLDIFERKIGEVCSQMFTKIIQSQKKIVESALNEKVDLTFYLSIKNLADKLNDGQVSRFGIVGSSFLKNPGLCDTVVVVEMWLLISKFINLLSKESSDFVEFWGHVEKFLDGTYQTSLMNDKRKDDILGSNTQTRDDYGQFLEMKNEQIRCIRRRGEDFLKRLCENLSNFFNASQDTLSTGSIPEKETGIPLDYGFLPPNSNCLSCLRYLPRIVEPILKFTTELAQLNVSSSSIEFLRETNALVLERSISAISATKLRDISSFHTLEDWSMYRTSGSEEYGITQFPEVVQTFQNLSITTVRNMLYSYEKLPVLNGIYVVTHPSRKMLAGIEVQQIVSMEAVLESILKEAAKEKENPRNPRTILTLTNLQYIKGSVFPQVLRYFDESFETQLSRKNLEIFTLLAKMETSILGNYLSDLKVTLRDILEERFYEINWAAYNSNSFRVGDYIIEALMVLVRVHSECFKIAPQLIGRILRESQVFISKYLFEAFKPYAGNLSSDGLLQVVVDLQFFLRVLSNMLETTTEATINATLQNCFENDLDRMQRCIKETEPIVSANLEKTSVQFASFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5E1Z9</accession>
    <name>C5E1Z9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0H00968p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0H00968g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555922.1">
        <property type="nucleotide sequence ID" value="XM_002555876.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E1Z9"/>
    <dbReference type="FunCoup" id="C5E1Z9">
        <property type="interactions" value="296"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E1Z9"/>
    <dbReference type="GeneID" id="8294234"/>
    <dbReference type="KEGG" id="lth:KLTH0H00968g"/>
    <dbReference type="eggNOG" id="ENOG502RIXM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015493_1_1_1"/>
    <dbReference type="InParanoid" id="C5E1Z9"/>
    <dbReference type="OMA" id="MPGTIDP"/>
    <dbReference type="OrthoDB" id="5419315at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF7">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="4">
        <location>
            <begin position="26"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="90"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="426"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="426"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="448"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR30060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="623" mass="69173" checksum="9ED9FBF88018E610" modified="2009-07-28" version="1">MAEAPQPRNNAISVTACAKRTHRKTGCVPCKIRKKRCSEHKPVCTDCQRLGFYCVYLPEKCPKEKVQYYREKVEKQLLEKKTTLDRRKASGVVQAEAQSSSINEVVPEESGGTSVSNDEKEGQHVVSNDGLDESQRLLDVTNVAELLGADGGSDSLIPGLLSLSGSPLSPRTLASTPAPSLSDPVLAELDDTALHLYQYYRDRLALIVSIAPSQQNHYRKIFLPMAHQNEGVMYGLLAWAARHLSLTVDENDTALDGSAAPSSLVAGPLTDPRYTELANKYTLFSLQRLSYRNSGFLMSLAQILVLCGAEICQGDVTRWQTLLQCATSMIKEHAPDGDIGALLAKSELASSLADTQTTRWLLSNFVYHDIMGSDQTHFPMSQYARVLQNPMNPLESPVDPLHGVNRPVFQILGQVKNLARQVKQELRQELQRSGDLDEKDETRDPRDSASPTAAPAPSRETMAIAQELQGALYRISPSESDLAWYDNFAEIGSDIRPLAETLFSVFRTAALILLKTAVLRQTTDSYEIRFLVSQLSDELDCVLGSQLEGGLCFPLFICGINAHDRAQRISVEIKFADFIKRYKFRNVERALVVMRQLWESADNGVTQDWFDIVDSVGWDLNFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5E232</accession>
    <name>C5E232_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 20">Autophagy-related protein 9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ordered locus">KLTH0H01760g</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="20">Phospholipid scramblase involved in autophagy. Cycles between the preautophagosomal structure/phagophore assembly site (PAS) and the cytoplasmic vesicle pool and supplies membrane for the growing autophagosome. Lipid scramblase activity plays a key role in preautophagosomal structure/phagophore assembly by distributing the phospholipids that arrive through ATG2 from the cytoplasmic to the luminal leaflet of the bilayer, thereby driving autophagosomal membrane expansion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate)(in) = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate)(out)</text>
            <dbReference type="Rhea" id="RHEA:67920"/>
            <dbReference type="ChEBI" id="CHEBI:58088"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) = a 1,2-diacyl-sn-glycero-3-phospho-L-serine(out)</text>
            <dbReference type="Rhea" id="RHEA:38663"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine(in) = a 1,2-diacyl-sn-glycero-3-phosphocholine(out)</text>
            <dbReference type="Rhea" id="RHEA:38571"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out)</text>
            <dbReference type="Rhea" id="RHEA:38895"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Golgi apparatus membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3 20">Preautophagosomal structure membrane</location>
            <topology evidence="3 20">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 20">Belongs to the ATG9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555955.1">
        <property type="nucleotide sequence ID" value="XM_002555909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E232"/>
    <dbReference type="FunCoup" id="C5E232">
        <property type="interactions" value="273"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E232"/>
    <dbReference type="GeneID" id="8294268"/>
    <dbReference type="KEGG" id="lth:KLTH0H01760g"/>
    <dbReference type="eggNOG" id="KOG2173">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006200_1_0_1"/>
    <dbReference type="InParanoid" id="C5E232"/>
    <dbReference type="OMA" id="IAEWKFR"/>
    <dbReference type="OrthoDB" id="2020634at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034497">
        <property type="term" value="P:protein localization to phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007241">
        <property type="entry name" value="Autophagy-rel_prot_9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13038">
        <property type="entry name" value="APG9 AUTOPHAGY 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13038:SF10">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04109">
        <property type="entry name" value="ATG9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 20" id="KW-0072">Autophagy</keyword>
    <keyword evidence="15" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="13 20" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="14 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7 20" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="486"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="571"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="677"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="92"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="748"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="826"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="26"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="68"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="92"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="835"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="852"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="867"/>
            <end position="876"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00024479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00024615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00024621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00024631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU364027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="CAR30093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="921" mass="105040" checksum="EB8F1BB19449CD78" modified="2009-07-28" version="1">MDGSSSKYHEPERNTFLSRVFGPHSTEGNSMGNTEMTQIQASEQSFVQSGGMRMIESDQGSSSDDDASFEREEDDSRVRAFHGIQSVVLDADSDAGSSSGAGSIASVQKLGQGADSDSEYQERRPGAEEDNNDEDSPLFRQNLKSLDFKSPSPVPAGPSVGFGPEVSDRRAFKSKFIDKHTQFLKLGKDEAEAESFLFRKNSAWDEENQQPKAQTNVPRIFGNFAAIGNTPAARLHTLSPRERALWKWANVENLDIFLQEVYNYYLGNGFYCICIEKALNIATLLFVVFISTFMGCCIDYSKLPHSTKISEVYVHKCYSTQISGTTKVLLWGFYIFVALKVAQFYFDVRALRDIHNFYSFLLNISDKELQTVSWQKVIKQIMLLKDQNAVTANVAEVKAKNRIDAHDVANRIMRRENYLIALFNNDILDLTLPIPLYRTSTLTKTLEWNLNLCIIGFAFNESGYLKQSFLKSSQRSYLEEELRKRFMLAGFLNIILSPFLVAYFILLYFFRYFNEYKTSPSALSSRQYTPIAEWKFREYNELYHLFQKRLGLSVEVANNYIDQFPKEKTNIIMKFIAFVSGSFVAILALLTVFDPENFLNFEITKDKSVLFYISVFGTVWAICKNSVSGQYRVFDPEESLREVVSYLHYKPKEWEGKYHTEDVKQDFCKLFNLRVILLLRELASLVMTPFILWFSLPKSSGKIVDFMREVSVYVDGLGYVCKYATFEVQGRHHDAKSRASRIIKNHQNFDAPQGYDESEHSSASDSEDDDKSVNKMMRSYMYFLDSYQNNDNEVGKHQLPKSGRNGKGPGNVDWLNTNYSWKRQFQPGQRPGDFQGNNVGYSSSMPTGTKDPVTKRSSEFKADSRSTGRGQTRFDSSLGESFINSVPLGEYRHLDRAEEVPGGNGVLGLLNQYYKKSDVGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5E270</accession>
    <name>C5E270_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S ribosomal protein L36</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0H02596g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL36 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555993.1">
        <property type="nucleotide sequence ID" value="XM_002555947.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E270"/>
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        <property type="interactions" value="981"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E270"/>
    <dbReference type="GeneID" id="8294306"/>
    <dbReference type="KEGG" id="lth:KLTH0H02596g"/>
    <dbReference type="eggNOG" id="KOG3452">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140672_1_0_1"/>
    <dbReference type="InParanoid" id="C5E270"/>
    <dbReference type="OMA" id="NKGHKTE"/>
    <dbReference type="OrthoDB" id="9616667at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1760:FF:000003">
        <property type="entry name" value="60S ribosomal protein L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1760">
        <property type="entry name" value="60S ribosomal protein L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000509">
        <property type="entry name" value="Ribosomal_eL36"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038097">
        <property type="entry name" value="Ribosomal_eL36_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10114">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01158">
        <property type="entry name" value="Ribosomal_L36e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01190">
        <property type="entry name" value="RIBOSOMAL_L36E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR30131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="100" mass="11080" checksum="6ECD94673D8EBD43" modified="2009-07-28" version="1">MAVKSGIATGLNKGKKVTQMTPAPKISYRKGASSNRTKFVRSLVKEVAGLAPYERRLIDLIRNAGEKRARKVAKKRLGTFGRAKAKVEEMNDIITASRRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2D4</accession>
    <name>C5E2D4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase</fullName>
            <ecNumber evidence="4">4.1.3.44</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">tRNA wybutosine-synthesizing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ordered locus">KLTH0H04026g</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>N(1)-methylguanosine(37) in tRNA(Phe) + pyruvate + S-adenosyl-L-methionine = 4-demethylwyosine(37) in tRNA(Phe) + 5'-deoxyadenosine + L-methionine + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:36347"/>
            <dbReference type="Rhea" id="RHEA-COMP:10164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10165"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64315"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="EC" id="4.1.3.44"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">tRNA modification; wybutosine-tRNA(Phe) biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TYW1 family.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.44" evidence="4"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556057.1">
        <property type="nucleotide sequence ID" value="XM_002556011.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2D4"/>
    <dbReference type="FunCoup" id="C5E2D4">
        <property type="interactions" value="690"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2D4"/>
    <dbReference type="GeneID" id="8294370"/>
    <dbReference type="KEGG" id="lth:KLTH0H04026g"/>
    <dbReference type="eggNOG" id="KOG1160">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007952_1_2_1"/>
    <dbReference type="InParanoid" id="C5E2D4"/>
    <dbReference type="OMA" id="FHVNGKW"/>
    <dbReference type="OrthoDB" id="271553at2759"/>
    <dbReference type="UniPathway" id="UPA00375"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102521">
        <property type="term" value="F:tRNA-4-demethylwyosine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031591">
        <property type="term" value="P:wybutosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000196">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013917">
        <property type="entry name" value="tRNA_wybutosine-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034556">
        <property type="entry name" value="tRNA_wybutosine-synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13930">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT TRNA 4-DEMETHYLWYOSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13930:SF0">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT TRNA 4-DEMETHYLWYOSINE SYNTHASE TYW1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00258">
        <property type="entry name" value="Flavodoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08608">
        <property type="entry name" value="Wyosine_form"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00284">
        <property type="entry name" value="tRNA_wybutosine-synthesizing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01071">
        <property type="entry name" value="tRNA_wybutosine-synthesizing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="12" id="KW-0456">Lyase</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="Flavodoxin-like" evidence="18">
        <location>
            <begin position="179"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="19">
        <location>
            <begin position="436"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="759"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="762"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="773"/>
            <end position="784"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00071388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00077859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CAR30195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="784" mass="87107" checksum="9874C512A8493942" modified="2009-07-28" version="1">MELSTKLTDHLVVCAVVAVYLISGGRLSIGISVAVLYALAIHDSGRKVEVSEAGIIQAKSGVAGKPEKKTCCGGKGGSSCCSSGSGGKKGGCCGGSCASKKRAEEKPASTGPVFESSASEPIAIDFTHAFKPARSSKPKSGAKKMPRFISRTAGSAKKDTGFFKKALTVSNEKLSNSQIYVFYSSLQGAAERSAKTVLETLSGMPELKYEPKLLNLDELSDFDDYFVNVPCSYALYILVLPSYDSDSPLDFFLQSLQENASDFRIDKYVLRKLVGYAVLGIGDSESWPEKFCYQARQADLLLAKQGGRRVFPVGEICMKFGGDPKVSEWSKLLAETLCDDEPILYEYDETLDDEEPQDPEDELTKEPLIDLEDVGKSSELQDVSSVKKMIVKDSPTFKNLTKQGYTVVGSHSGVKICRWTKNDLRGRGSCYKHSLFNIVSSRCMELTPSLACSSKCIFCWRHGTNPVSKTWRWEVDDPEYILENALQGHYAKIKQMRGVPGVIAERFAKAFKVRHCALSLVGEPIMYPFISKFVSLLHQKEISSFLVCNAQHPEPLRDLGKVTQLYVSIDAPTKNELKKVDRPLYRDFWERLMECLDILRTTQSHQRTVFRMTLVKGFNMADVSAYADLVQRGLPCFIEVKGATFCGTSDGNGNPLTMQNIPFYEECAKFVKSLAAELQRRGLGYDVAAEHAHSNCILIASTKFKINNEWYTHIDFDRFFELLNSGKDFNHMEYLQKTPEWALFGNGGFAPGNTRFYKKKRGQEAQAKEEQGLQENVSTQPINA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2D8</accession>
    <name>C5E2D8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0H04114p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0H04114g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycerophosphoryl diester phosphodiesterase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556061.1">
        <property type="nucleotide sequence ID" value="XM_002556015.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2D8"/>
    <dbReference type="FunCoup" id="C5E2D8">
        <property type="interactions" value="123"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2D8"/>
    <dbReference type="GeneID" id="8294374"/>
    <dbReference type="KEGG" id="lth:KLTH0H04114g"/>
    <dbReference type="eggNOG" id="KOG2258">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030006_1_2_1"/>
    <dbReference type="InParanoid" id="C5E2D8"/>
    <dbReference type="OMA" id="RALPECW"/>
    <dbReference type="OrthoDB" id="1058301at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034479">
        <property type="term" value="F:phosphatidylglycerol phospholipase C activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046475">
        <property type="term" value="P:glycerophospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08570">
        <property type="entry name" value="GDPD_YPL206cp_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052271">
        <property type="entry name" value="GDPD-Related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42758">
        <property type="entry name" value="PHOSPHATIDYLGLYCEROL PHOSPHOLIPASE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42758:SF2">
        <property type="entry name" value="PHOSPHATIDYLGLYCEROL PHOSPHOLIPASE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="GP-PDE" evidence="8">
        <location>
            <begin position="4"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR30199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="321" mass="37996" checksum="FBC79C1B66F53EB3" modified="2009-07-28" version="1">MATAKIIGHRAYRGYYPENTILAFNKAKEANVDVIETDLQMSKDGVVVINHDQDTQRCWDKNYVISETNWDALQSLNCKDARFQDQKMPSLKQVLRWIVDNPSMKLMLDIKFTNDQEIMLKSVAEMLSTKEDLLFWRERVIWGLWTVDWFRYGMETGVIKDFQVACISLSLEVAKSFVEYSRELNSPHFRLSGVSMHFVSTWSKTFQKKWLHYFQQNNVEIFVWTINHAVDFKYCNALPISGFVTDFPVEARGAIGQYERSKTTFKRPFFISREGFRFYGFLVIYRLTIEFLFSPWAQYKVFGNFSFSLLFIKFMRIIHFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2G8</accession>
    <name>C5E2G8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">KLTH0H04840p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0H04840g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556091.1">
        <property type="nucleotide sequence ID" value="XM_002556045.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2G8"/>
    <dbReference type="STRING" id="559295.C5E2G8"/>
    <dbReference type="ESTHER" id="lactc-c5e2g8">
        <property type="family name" value="Acidic_Lipase"/>
    </dbReference>
    <dbReference type="GeneID" id="8294405"/>
    <dbReference type="KEGG" id="lth:KLTH0H04840g"/>
    <dbReference type="eggNOG" id="KOG2624">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010974_5_0_1"/>
    <dbReference type="InParanoid" id="C5E2G8"/>
    <dbReference type="OMA" id="PHGLGME"/>
    <dbReference type="OrthoDB" id="9974421at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000095">
        <property type="entry name" value="Triglyceride lipase-cholesterol esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025483">
        <property type="entry name" value="Lipase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11005">
        <property type="entry name" value="LYSOSOMAL ACID LIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000862">
        <property type="entry name" value="Steryl_ester_lip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="12">
        <location>
            <begin position="106"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="11">
        <location>
            <position position="201"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="11">
        <location>
            <position position="374"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="11">
        <location>
            <position position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000862-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR30229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="443" mass="51361" checksum="F78250CB96ACA615" modified="2009-07-28" version="1">MCGRMESLWNFCLSLCLRIPMWIYNHCILPLICVLPFFQKYKTFNYSYHNRLVKEVVHANDILDIAMAHGFDVQEHLVRTQDGYLLTVHRVLGKKSEVYRSQAKKPVVYFHHGLLTNSELFLLGQTSDKCLPFLLVERGYDVWLGNNRGNKYSRKHINLSSRSAKFWDFSLDEYAIFDIPNTISYILNLTGLEKLTYIGFSQGSSQAFAAFSINPQLRDKIQLFVGLSPAMIPRSLSHPVAKFLVTCPPELLYFIFGTRAILPSVVFWQRLFGPINYMKIVDWSLVYLFNWKSEHISSAQKQVGYTHMFSPSSVKSVAHWFQIIHNKRFQMFQEDISKIYSSVPSLHRKLHRCAPFPVQVIENMPMMLLYGDQDILIDMETTKQNLVRNLSDLTLVELPGYEHMDTLWAENVVEEVFNPILKKIDQIHAEKPYQNGLEVTASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2J8</accession>
    <name>C5E2J8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="17">KLTH0H05566p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">KLTH0H05566g</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family. ISWI subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556121.1">
        <property type="nucleotide sequence ID" value="XM_002556075.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2J8"/>
    <dbReference type="FunCoup" id="C5E2J8">
        <property type="interactions" value="1223"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2J8"/>
    <dbReference type="GeneID" id="8294436"/>
    <dbReference type="KEGG" id="lth:KLTH0H05566g"/>
    <dbReference type="eggNOG" id="KOG0385">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000315_0_2_1"/>
    <dbReference type="InParanoid" id="C5E2J8"/>
    <dbReference type="OMA" id="EFQFRES"/>
    <dbReference type="OrthoDB" id="5857104at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:ATP hydrolysis activity"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0140658">
        <property type="term" value="F:ATP-dependent chromatin remodeler activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
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        <property type="term" value="F:nucleosome binding"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0034728">
        <property type="term" value="P:nucleosome organization"/>
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    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17997">
        <property type="entry name" value="DEXHc_SMARCA1_SMARCA5"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
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    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000082">
        <property type="entry name" value="ISWI chromatin remodeling complex ATPase ISW1"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="3.40.50.10810:FF:000002">
        <property type="entry name" value="ISWI chromatin-remodeling complex ATPase CHR11 isoform A"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="1.10.10.60:FF:000234">
        <property type="entry name" value="ISWI chromatin-remodeling complex ATPase ISW2"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1190">
        <property type="entry name" value="iswi atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.30">
        <property type="entry name" value="ISWI, HAND domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Helicase_ATP-bd"/>
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    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044754">
        <property type="entry name" value="Isw1/2_DEXHc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015194">
        <property type="entry name" value="ISWI_HAND-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036306">
        <property type="entry name" value="ISWI_HAND-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
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    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
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    <dbReference type="InterPro" id="IPR015195">
        <property type="entry name" value="SLIDE"/>
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    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
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        <property type="entry name" value="SNF2/RAD54-like_C"/>
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        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SWI_SNF-RELATED MATRIX-ASSOCIATED ACTIN-DEPENDENT REGULATOR OF CHROMATIN SUBFAMILY A MEMBER 5"/>
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    </dbReference>
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    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101224">
        <property type="entry name" value="HAND domain of the nucleosome remodeling ATPase ISWI"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="148"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="15">
        <location>
            <begin position="446"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="16">
        <location>
            <begin position="825"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="78"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="623"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="727"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1017"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="880"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="103"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="624"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1017"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1074"/>
            <end position="1086"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAR30259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1106" mass="126816" checksum="F552BE498156C98D" modified="2009-07-28" version="1">MDDQEYLEKLKPFQEGLPPSDPESNKKRYLIKGQQKSEFDLEGTIKRFQHLLNLSGLFRHFIERKAAKDEKFQKVLNILDNPSGRKGSKGSSHQDKRRRKTETEEDQELLKDEEGEEQDQVGYQFRESPPFVNGQLRPYQIQGLNWLVSLHQSNLAGILADEMGLGKTLQTISFIGYMRYVEKKRGPFVVIAPKSTLNNWLREINRWTPEVNAFILQGDKEERAKLVSNKLMACDFDIVVASYEIIIKEKSSFKKIDWEYIIIDEAHRIKNEESMLSQVLREFTSRNRLLITGTPLQNNLHELWALLNFLLPDVFSDSQAFDDWFSSESSEEDKGTIVKQLHTVLQPFLLRRLKNEVETSLLPKKELNLYIGMSAMQKRWYKQILEKDLDAVNGANGSKESKTRLLNIMMQLRKCCNHPYLFDGAEPGPPYTTDEHLVYNSAKLKVLDKLLRKFKEEGSRVLIFSQMSRLLDILEDYCFFRNYEYCRIDGSTAHEDRIEAIDEYNAPDSKKFVFLLTTRAGGLGINLTTADVVVLYDSDWNPQADLQAMDRAHRIGQKKQVKVFRLVTDNSVEEKILERATQKLRLDQLVIQQSRNGVANKEAKKGDSKDALLSMIQHGAADVFSSNSGDSSTKGTPQPGSTDDVDGDIDLDNLLANSEDKTQSLNQKYEALGLDDLQKFNQDSAYEWNGQDFKKRANKDIISTAWINPSKRERKENYNIDGYYKDVLNPGRSTTPAQPKMPRPQPFHSHQLLSPQLKVLYEKERMWNTKKNNYVPTLEDVKTTYGEIEDEEEKQAKLNMLLLAVANAQPLTEEEEKQKAQLESEGYTNWNKNDFRKFIAACGKYGRNSIKAIAFEFAPHKTEEEVRDYAKAFWTNIQRVEDYERYLKNIEAEEDRIRRTKLQQEALRRKIAQCSNPLFDLTLKHPPSNSNKRVFSEEEDRFLLLMLFKYGIDRENVYELVRDEIRDSPLFELDFFFQSRTPIELNRRAITLLQCLEKEFNAGVVLDDGMKRRLAEEDENAKNMAEEVKEGAAEAGSEAAPGPGGADVAGKAMEPAPANGELNGKRPREEEDTAATAVAAAMTSTEPGPADKPSVNGDAKKPKTEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2U6</accession>
    <name>C5E2U6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0H07810p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0H07810g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAPT1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556219.1">
        <property type="nucleotide sequence ID" value="XM_002556173.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2U6"/>
    <dbReference type="FunCoup" id="C5E2U6">
        <property type="interactions" value="287"/>
    </dbReference>
    <dbReference type="GeneID" id="8294537"/>
    <dbReference type="KEGG" id="lth:KLTH0H07810g"/>
    <dbReference type="eggNOG" id="KOG2490">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003655_1_1_1"/>
    <dbReference type="InParanoid" id="C5E2U6"/>
    <dbReference type="OMA" id="YHDVRGQ"/>
    <dbReference type="OrthoDB" id="5376140at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008010">
        <property type="entry name" value="Tatp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13317">
        <property type="entry name" value="TRANSMEMBRANE ANTERIOR POSTERIOR TRANSFORMATION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13317:SF4">
        <property type="entry name" value="TRANSMEMBRANE ANTERIOR POSTERIOR TRANSFORMATION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05346">
        <property type="entry name" value="DUF747"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="110"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="181"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="290"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="350"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="381"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="458"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="30"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="458"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR30357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="498" mass="56919" checksum="63A765F6B8865817" modified="2009-07-28" version="1">MTKRISKPKKPKRSVHWKYIKEILLAEFEVKDAKRKDQSAEDDQREAEEAEHEMEQILNMLRLFLHMEKLMLFSLLACLNAFLYYFTTFPARLLYNFVGRKKLRQADREFNLLALIALASLVLFRVDTSRVYHRIKGQSAIKLYMMFGVLEMCDKMLSSVGQSLMVIVQTKRTRQTKLQKALLNMCALGYLSCHGYILMYETIALNVAVNSYSNSLLTLLLSMQFAEIKASVFKRFDKEGLFQITISDIIERFQMVILLTIIALRNLVATSTSFSTLIPDSWSWNSTSSIVVGILCGPVLTVVGSEILVDWIKHAYITKFNRIRPQMYDTYLRIISHDHMCNLQKLQIRLGLPVPALVVLFIVMIRPSLKIGLFEVSTSVAGSVAMVLMAFVCLFLTKFVLYLVLARWSQAMQANGLTGSTDTKVTEEMYVPGTPSSGMGKIDEQLRAQLHATDAQQIDTKQKQASVPPSSNEIRAQRDSKRNLKNVTRYKMVSKRIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2W1</accession>
    <name>C5E2W1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">succinate-semialdehyde dehydrogenase [NAD(P)(+)]</fullName>
            <ecNumber evidence="6">1.2.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0H08162g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>succinate semialdehyde + NAD(+) + H2O = succinate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13217"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57706"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.2.1.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>succinate semialdehyde + NADP(+) + H2O = succinate + NADPH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13213"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57706"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.2.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid degradation; 4-aminobutanoate degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.16" evidence="6"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556234.1">
        <property type="nucleotide sequence ID" value="XM_002556188.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2W1"/>
    <dbReference type="STRING" id="559295.C5E2W1"/>
    <dbReference type="GeneID" id="8294552"/>
    <dbReference type="KEGG" id="lth:KLTH0H08162g"/>
    <dbReference type="eggNOG" id="KOG2451">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_5_3_1"/>
    <dbReference type="InParanoid" id="C5E2W1"/>
    <dbReference type="OMA" id="ARIMTCE"/>
    <dbReference type="OrthoDB" id="310895at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004777">
        <property type="term" value="F:succinate-semialdehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009450">
        <property type="term" value="P:gamma-aminobutyric acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07103">
        <property type="entry name" value="ALDH_F5_SSADH_GabD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000004">
        <property type="entry name" value="Succinate-semialdehyde dehydrogenase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000005">
        <property type="entry name" value="Succinate-semialdehyde dehydrogenase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050740">
        <property type="entry name" value="Aldehyde_DH_Superfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43353">
        <property type="entry name" value="SUCCINATE-SEMIALDEHYDE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43353:SF5">
        <property type="entry name" value="SUCCINATE-SEMIALDEHYDE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="9">
        <location>
            <begin position="39"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00050387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00052698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00067047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR30372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="506" mass="55036" checksum="520ADD7FECE66EEA" modified="2009-07-28" version="1">MLRFKQSLIRPKTLFKRFASTSFEDPELFRTKALVNGKWLSGDDQFPVINPSTSEEISTITNCGVADYNEAIGHAHRAFKKFKETSPRERSQVLYNIYDLMSEHKNDLARLITMENGKTFKDSLTEIDYSASYFRWFAEEAPRIYGHVIPSSSSLRKQIITLRQPLGVVGIMAPWNFPSAMIARKLAPVIATGNACVIKPPHESPMSALALGWLAEQAGLSDGVCNVLPTTHTAAIGEYICSHDQVRKITFTGSTKVGKLLMKQCANGNIIKKVSMELGGNAPFIVFDDADIDKAVNGLISCKFRQLGQTCICANRVFVHESIYGDFATRLVAAIESGFHLGDGFSPKVTHGPLINEGAVQKVSELVEDAKNKGANVLTGGVRASSLGPRFYHPTVLSGVDETMSIFHTEVFGPVASLIGFKTTEEVIERANKTNVGLAGYFYSSNIQTVLDVASELEVGMVGVNSSAISDAAMPFGGIKNSGLGREGSSWGVDDYTDLKSIVLEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2X4</accession>
    <name>C5E2X4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0H08514p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0H08514g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Bud neck</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556247.1">
        <property type="nucleotide sequence ID" value="XM_002556201.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2X4"/>
    <dbReference type="FunCoup" id="C5E2X4">
        <property type="interactions" value="562"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2X4"/>
    <dbReference type="GeneID" id="8294568"/>
    <dbReference type="KEGG" id="lth:KLTH0H08514g"/>
    <dbReference type="eggNOG" id="KOG2655">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017718_8_0_1"/>
    <dbReference type="InParanoid" id="C5E2X4"/>
    <dbReference type="OMA" id="RSNPMGS"/>
    <dbReference type="OrthoDB" id="416553at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032161">
        <property type="term" value="C:cleavage apparatus septin structure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031105">
        <property type="term" value="C:septin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005940">
        <property type="term" value="C:septin ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000281">
        <property type="term" value="P:mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01850">
        <property type="entry name" value="CDC_Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000162">
        <property type="entry name" value="septin-7 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016491">
        <property type="entry name" value="Septin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006698">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="9">
        <location>
            <begin position="95"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="473"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="476"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR30385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="494" mass="55868" checksum="582BD8D1392EC1CA" modified="2009-07-28" version="1">MQAGEPIEELKSAASTENEFGSVSADVKEENMPDAIEPASSVNGHGSDVASEGEVQQKGQVLPKQPELRVLHHKISGYVGFANLPKQWHRKSIRRGFSLNLLCVGQGGLGKSTLINTLFNKQLYDLEGKPTEDLAALKLEDSENLSESLERPQGEEQPSVKIETISTDIEENGVKLALTVVDAPGFGDAIDNTDSWVPIVREIDSRFDQYLDAENRIDRDVIQDHRIHACLYFIEPTGHCLAPLDLEFCKAVHQKCNLIPVIAKSDILTDEEIEQFKHTIKTQLDNAGVELFEPPQYSLDDKDTVARCAALCTRMPFAVVGSTSEVSTPDGRRVRGRSYPWGVIEVDNESHSDFVHLRDLLIRLYLMELREKTENVLFERYRSEKLMSLGIKQDNSVFKEYDPELKQKEEKQLHEAKLAKLEAEMKAVFQQKVNEKEKKLQKSEAELFARHKEMKDKLLKQLKALEDKKHQLEMSYANQSANSPAQTKKKGFLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2023-06-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>C5E395</accession>
    <name>C5E395_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0H11462p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0H11462g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556368.1">
        <property type="nucleotide sequence ID" value="XM_002556322.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E395"/>
    <dbReference type="GeneID" id="8294701"/>
    <dbReference type="KEGG" id="lth:KLTH0H11462g"/>
    <dbReference type="HOGENOM" id="CLU_1235214_0_0_1"/>
    <dbReference type="InParanoid" id="C5E395"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR30506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="224" mass="24843" checksum="3649329E492A6FC4" modified="2009-07-28" version="1">MPPLYFRLYDLSSGREANRSAGFWLLGELAQQLHARCRGYQATNWLARLVAAVPQWASGAHSPYVPRSGSFTVRRICICPSRPAPAESTGDTRARGNGLQETHVYAAAAWANAYVRTHACVLHSQHARGIRVTPSPVQTEAGNRDSQGLLDRKRHSHVTTNTITWTNGPTQPYFFRAAARSGARVSSCVIVLHFSLYSPFLHGGWRTGEKIMYIKVETSYVCDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-04-02" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5E396</accession>
    <name>C5E396_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0H11484p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0H11484g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556369.1">
        <property type="nucleotide sequence ID" value="XM_002556323.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E396"/>
    <dbReference type="STRING" id="559295.C5E396"/>
    <dbReference type="GeneID" id="8294702"/>
    <dbReference type="KEGG" id="lth:KLTH0H11484g"/>
    <dbReference type="eggNOG" id="KOG1181">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_310808_0_0_1"/>
    <dbReference type="InParanoid" id="C5E396"/>
    <dbReference type="OMA" id="WHAGAER"/>
    <dbReference type="OrthoDB" id="9932926at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="816"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="162"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="227"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="273"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="303"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="330"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="374"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="403"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="525"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="559"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="590"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="617"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="654"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="698"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="743"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="758"/>
            <end position="768"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR30507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="946" mass="99347" checksum="DE3BBCD3BC5E5EF6" modified="2009-07-28" version="1">MDGIEDFMLTQDQPTTSEDKATMPLSPPEEQASQRPESHAAAVAPAPDHKTCAGEPQRTIEAAGALASGGENEAIEVEETNVDSDNNSRLRTKPSDLGRGHQDGDAAVAEAVAEPSPNIELGASAQGSIDAHADEVPKHEEPSALNPVGETPDRKPQRKQSLKREEHSKDESEHEGPDETSAGSRVASCGEIAKKNYQGGATIKGGERSGAAPLKAESKPNNNRNGRVPDKKDEADKTDDADLKNGTLIERKKNNAGLDEDLQPAGTDAAAENLEKASGEIKEDASGKCEKGAESADEDASVIEQQTQTQSANKAVGSGAAADSTPDLRGSAKGEEREEHERALDGGDAPNAHDTVEAREPANGELAMGEEAVELTSAEPTETAEAVEPTSAEPTETAEQAEEAEHPAQRAESRGAELSHRPKTSDVRESREPEVPEAGDAELVGRKEAHAAQQDDEGLAGRDGQAAHALQDKQGIQATIALQGEHENPAGAAGLASDADPSEQKHNDRANLGAETAAAQTENPEPNKARGTDRPETLRHTERKEATCTDPAQTTQCDSGSAVLGAAAASRNRDAQQPGFRAATHTKDKEDEDATTEDQAQETGPEANSDLISDLLSELHLEPQPDPRSHVVAQTEPEAGLDHNKQTQASRPKQSPTASSSADQSSDPENQLNATDGAADDVAFDADGSAEPPAARVSAANSESASPAQSSTSEPAAVPSGSNTPPTHVKPGPAVPESINNDQRAEVTKQDVPDAEHQQTSVDSSGNHAKSGPAPAHPDAPSPLSAARDSDAPAQSQSRALADEIDDLLRGLEASSGVADKSSAEKGAARSPSVGGDKNKSISAISAISDILASEPVYLYTSLAGGGFHMPSRTNRLAHILSANRVDFSYRDLGTDDEARRVWKRFGRGRSLPAVVRGRDDIIGNWEEMDQANEDYRVRELIYETL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3D8</accession>
    <name>C5E3D8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0H12562p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0H12562g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556411.1">
        <property type="nucleotide sequence ID" value="XM_002556365.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3D8"/>
    <dbReference type="STRING" id="559295.C5E3D8"/>
    <dbReference type="GeneID" id="8294751"/>
    <dbReference type="KEGG" id="lth:KLTH0H12562g"/>
    <dbReference type="eggNOG" id="KOG2130">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016785_1_2_1"/>
    <dbReference type="InParanoid" id="C5E3D8"/>
    <dbReference type="OMA" id="WPAYKNW"/>
    <dbReference type="OrthoDB" id="424465at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000987">
        <property type="term" value="F:cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041667">
        <property type="entry name" value="Cupin_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050910">
        <property type="entry name" value="JMJD6_ArgDemeth/LysHydrox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12480">
        <property type="entry name" value="ARGININE DEMETHYLASE AND LYSYL-HYDROXYLASE JMJD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12480:SF21">
        <property type="entry name" value="JMJC DOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13621">
        <property type="entry name" value="Cupin_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="JmjC" evidence="1">
        <location>
            <begin position="277"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR30549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="577" mass="65928" checksum="0AB1BFD266B127D8" modified="2009-07-28" version="1">MAVDCKRAKKIPNYRIDAHTIAPIHPLGVKPSGNIYLHNNLKELDLSRKRQLGHLAALSEEILALALSFIDSPSDLVSLGHSSRVLYAYTYNEELWRKLYIDEFMRLEAKGGNVPNSSGYHPYGCRKWRGSWRKTLLKLDEEALIQVNDLVFSDFLYRPYQCSKIDYEYLFKRVIAFEENSSALRHTLNPEFGVERIKEADLTLETFERSYCSKPFILCGEGDEKRWPLWDLEYLVGRFGDINFRQEAVEWKLSYYANYSRNNNDESPLYLFDCSSEAIQTLKGEYMAPKIFTYDLFKAFEGQEINCRPDHRWLIVGKAGSGSTFHKDPNQTSAWNAGLTGKKLWMMLPPDVKPPGVSTDKEEEEVTSPVGVGEWVLSGYYNDAVKLAQEGKCQIAVTFPGECIYVPSGWWHTVINLTDSVALTENFVPAPILSKVLLFFKNKKKQISGFHLQDTVNAMKRFLSAHPMKSDSSPNYEVIENFLEKCRHHALNNEDCGLIDLDLEPPIFEFFVELVRESKYGKHTERALADMHELEVNAARQEARTNPPATKTSEAWTNLAAESGSNFSFGFSFEETA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3E0</accession>
    <name>C5E3E0_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Citrate synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0H12606g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the citrate synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556413.1">
        <property type="nucleotide sequence ID" value="XM_002556367.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3E0"/>
    <dbReference type="FunCoup" id="C5E3E0">
        <property type="interactions" value="1010"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3E0"/>
    <dbReference type="GeneID" id="8294753"/>
    <dbReference type="KEGG" id="lth:KLTH0H12606g"/>
    <dbReference type="eggNOG" id="KOG2617">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022049_2_1_1"/>
    <dbReference type="InParanoid" id="C5E3E0"/>
    <dbReference type="OMA" id="VLEWLFK"/>
    <dbReference type="OrthoDB" id="8017587at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046912">
        <property type="term" value="F:acyltransferase activity, acyl groups converted into alkyl on transfer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006101">
        <property type="term" value="P:citrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.230.10:FF:000001">
        <property type="entry name" value="Citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.580.10:FF:000001">
        <property type="entry name" value="Citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.580.10">
        <property type="entry name" value="Citrate Synthase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.230.10">
        <property type="entry name" value="Cytochrome P450-Terp, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016142">
        <property type="entry name" value="Citrate_synth-like_lrg_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016143">
        <property type="entry name" value="Citrate_synth-like_sm_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002020">
        <property type="entry name" value="Citrate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019810">
        <property type="entry name" value="Citrate_synthase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010109">
        <property type="entry name" value="Citrate_synthase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036969">
        <property type="entry name" value="Citrate_synthase_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01793">
        <property type="entry name" value="cit_synth_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007128">
        <property type="entry name" value="PRK09569.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11739">
        <property type="entry name" value="CITRATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11739:SF8">
        <property type="entry name" value="CITRATE SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00285">
        <property type="entry name" value="Citrate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00143">
        <property type="entry name" value="CITRTSNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48256">
        <property type="entry name" value="Citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00480">
        <property type="entry name" value="CITRATE_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="active site" evidence="3">
        <location>
            <position position="310"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="356"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR610109-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR30551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="477" mass="52637" checksum="ACE17C65297BC7B2" modified="2009-07-28" version="1">MSVLASTRNTLARGLLQQSNSGRASVLLTVGGARLYSNGEKTLKERFAEIIPAKAEQIKQLRQEHGSTVIGEVVLNQAYGGMRGIKGLVWEGSVLDPDEGIRFRNRTIPDIQKELPKGAGGTEPLPEALFWLLLTGETPTESQVKALSADLASRSELPEHVSQLLDSLPKDLHPMAQFSIAVTALESESKFSKAYAQGVSKKDYWNYAFEDSMDLIGKLPVIASKIYRNVFKDGKLGSVDPNADFGKNLANLLGFKNDEFVELMRLYLTIHADHEGGNVSAHTTHLVGSALSSPYLSFSAGLNGLAGPLHGRANQEVLEWLFKLREEVKGDYSKETIEKYLWDTLNSGRVVPGYGHAVLRKTDPRYTAQREFALRHFPDYDLFKLVSTIYEVAPGVLTKHGKTKNPWPNVDSHSGVLLQYYGLTEASFYTVLFGVSRALGVLAQLIIDRAVGQPIERPKSFSTEKYAELVKSINAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3E2</accession>
    <name>C5E3E2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sterol regulatory element-binding protein cleavage-activating protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0H12672g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat SCAP family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556415.1">
        <property type="nucleotide sequence ID" value="XM_002556369.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3E2"/>
    <dbReference type="STRING" id="559295.C5E3E2"/>
    <dbReference type="GeneID" id="8294756"/>
    <dbReference type="KEGG" id="lth:KLTH0H12672g"/>
    <dbReference type="eggNOG" id="KOG1933">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_264915_0_0_1"/>
    <dbReference type="InParanoid" id="C5E3E2"/>
    <dbReference type="OMA" id="EFQFRPM"/>
    <dbReference type="OrthoDB" id="1914839at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032936">
        <property type="term" value="C:SREBP-SCAP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045540">
        <property type="term" value="P:regulation of cholesterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032933">
        <property type="term" value="P:SREBP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008202">
        <property type="term" value="P:steroid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030225">
        <property type="entry name" value="SCAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000731">
        <property type="entry name" value="SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46378">
        <property type="entry name" value="STEROL REGULATORY ELEMENT-BINDING PROTEIN CLEAVAGE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46378:SF1">
        <property type="entry name" value="STEROL REGULATORY ELEMENT-BINDING PROTEIN CLEAVAGE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50156">
        <property type="entry name" value="SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="234"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="264"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="297"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="379"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="566"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="14">
        <location>
            <begin position="230"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR30553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1211" mass="134935" checksum="412DC6CEB30908EF" modified="2009-07-28" version="1">MSAILQRITPQANFFNLSRLAVRYPVISIFLPLLILTIIAYPTLSSAWSGDITSRQYLSRVEFEPFFTGSSANLSLVQVWIKHLNEDNILKKSSLIESLALQNRLLRNTAGSDLVEVLQSPFQLWNNSLQVLQKDRSPLKTINYGANSIPKLLLRRVLKVNGFVTSAENMVINILTPGDKQDALKSILAQNVAELQSLSNVTGFKIATSSMLSNGNAPQVSFRFDILPMSRLDFLVSGTLCFLLILQLLRAYKSIVWVKHKYGIALAMLAEVTVGTAASATITGFFFKGYGENNPTFLGWLPITLISTSGLLRLLKETAGSSVTDSRKSYPMVASKEEPGRKGTKKYLVASAQVNASVFRTTILAIATSTFLFPFNRQLSFFFSAALLNTFILQLTFFTSVLSLDYRMLSSNDVLFLKNSNEIDARSLDEEFYGHNVNLKAYVKSLAIGWDTMLSKPLYFCMPYFFYINLKFRSVRSSSSLVYKFVHGQFSKAVSFSSARSPALIDKKSVSQLLFQKHGKGTFNVAFLPSDTIFALKGDVPDTNSLSGSYLGALQTPFVSSYKFDFYFFIEFIITVVLLSSLALLVLQIMLSRVETPIKSEDDPEVFNAVDEMQSRKETGSIMKADETAFHTKELSHGGHTLDIVSIPTSDSPFIFSLGIDRKLFVWSPLSNPVPPPTEIPLSKQLWPVARTVTSSNGNLTAILSEGGKVACWSRRKMCFLWSIKLDHPGKTVLELFFRKRTIPAFMKNRPTGNTSTSAGKIRDQIGVTSLSMGRKDSNVSVSSMKSSSSLANVFDARYNDKEAASIHDEDDGTELIFVTTDGSLTCIDISGKPVVSKVISSPVELASCKKLVTPRVNDRLVFCDQRGKIYVSTVVNNKWRTRELHIIRDSFNRGQKLMTPATLKRPSEVLGNGLYSPNSGSEIFENITLLLIPFVGMLVLARGNKAELVDAQTGTSIRSFTLSDVEPKTLKVFHDQPTHCRFCGSASVASLSIAYTEKSTKTLVMHTFKLESRTKTSICLRVERDPREIRCLGLESVVEKKHYMEEADCWNVTDNNLIIGLKRRNKLNQAENNSHAIVRSVSEGVRTGDCKLRNRVESRSMKHSASLEYNIHEIWEGWTMTVNGKVQYYKIPAGVNGLLTNRIGPVEKFGAKAIVAAFGNIMKLFYLGREELILATDGTNTNEDDTGLNFVNKRRKRLSERKTSNIYTQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3J6</accession>
    <name>C5E3J6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">2-hydroxyacyl-CoA lyase</fullName>
            <ecNumber evidence="9">4.1.2.63</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0H14102g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 2-hydroxy-3-methyl fatty acyl-CoA = a 2-methyl-branched fatty aldehyde + formyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:25375"/>
            <dbReference type="ChEBI" id="CHEBI:49188"/>
            <dbReference type="ChEBI" id="CHEBI:57376"/>
            <dbReference type="ChEBI" id="CHEBI:58783"/>
            <dbReference type="EC" id="4.1.2.63"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:25376"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>an (R)-2-hydroxy-long-chain-fatty acyl-CoA = a long-chain fatty aldehyde + formyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:67444"/>
            <dbReference type="ChEBI" id="CHEBI:17176"/>
            <dbReference type="ChEBI" id="CHEBI:57376"/>
            <dbReference type="ChEBI" id="CHEBI:170012"/>
            <dbReference type="EC" id="4.1.2.63"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:67445"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the TPP enzyme family.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.63" evidence="9"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556469.1">
        <property type="nucleotide sequence ID" value="XM_002556423.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3J6"/>
    <dbReference type="FunCoup" id="C5E3J6">
        <property type="interactions" value="441"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3J6"/>
    <dbReference type="GeneID" id="8294831"/>
    <dbReference type="KEGG" id="lth:KLTH0H14102g"/>
    <dbReference type="eggNOG" id="KOG1185">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013748_3_3_1"/>
    <dbReference type="InParanoid" id="C5E3J6"/>
    <dbReference type="OMA" id="PGPYGCL"/>
    <dbReference type="OrthoDB" id="10006023at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106359">
        <property type="term" value="F:2-hydroxyacyl-CoA lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001561">
        <property type="term" value="P:fatty acid alpha-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02004">
        <property type="entry name" value="TPP_BZL_OCoD_HPCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07035">
        <property type="entry name" value="TPP_PYR_POX_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045025">
        <property type="entry name" value="HACL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000399">
        <property type="entry name" value="TPP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43710">
        <property type="entry name" value="2-HYDROXYACYL-COA LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43710:SF2">
        <property type="entry name" value="2-HYDROXYACYL-COA LYASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00187">
        <property type="entry name" value="TPP_ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme N-terminal TPP-binding" evidence="14">
        <location>
            <begin position="6"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme central" evidence="12">
        <location>
            <begin position="188"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="13">
        <location>
            <begin position="381"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR30607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="551" mass="59077" checksum="0D316A3ECA5FAD5C" modified="2009-07-28" version="1">MSAFPEYLSELLHGYGITAVFGIVGIPIVELADAMISHGKVKFYGFRNEQAASYAASAYGYLTGRPGVLLVVGGPGVIHALAGVYNSISNKWPLIVLAGSSEDSNRGGFQELDQIALLSKYVKYSSRLSNSNVDQSFYDALRQAVLGTPGVSYLDIPANVIDQEVEKNESFSRRHIAKISYQPTDAALQRVAKLLNANRDKNILCVIGKGATENAQQIRPFVDEFQLPFLSTPMAKGVVPDSHALNVSSARSLALKSADIVLVLGARLNWILHFGEAPKWKNDAIFIQIDTTAEELGHNNGAGTLFSLCGDIGTTVAKLTPLLAGFSGRGLFGSLETRIQQNEKRLRARENEPSPALTYNKVYATIRKQIVDKDTIIVSEGANTMDVARISFPTDYPKLRMDAGTNATMGVGLGYAIAAKIAHPQKNVIAIQGDSAFGFSGMELETASRYKLGITVVVMNNSGIYHGEDPAAESSPNTAPSTALSQECRYDLVAEGLGCRGFVARTQGEVSAAFAAAIKVAADGRPALINVMIEPGPQGKLSFGWQKKAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3L8</accession>
    <name>C5E3L8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Inositol-pentakisphosphate 2-kinase</fullName>
            <ecNumber evidence="3 9">2.7.1.158</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0H14630g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Has kinase activity and phosphorylates inositol-1,3,4,5,6-pentakisphosphate (Ins(1,3,4,5,6)P5) to produce 1,2,3,4,5,6-hexakisphosphate (InsP6), also known as phytate.</text>
    </comment>
    <comment type="function">
        <text evidence="9">Phosphorylates Ins(1,3,4,5,6)P5 at position 2 to form Ins(1,2,3,4,5,6)P6 (InsP6 or phytate).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>1D-myo-inositol 1,3,4,5,6-pentakisphosphate + ATP = 1D-myo-inositol hexakisphosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20313"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57733"/>
            <dbReference type="ChEBI" id="CHEBI:58130"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.158"/>
        </reaction>
    </comment>
    <comment type="domain">
        <text evidence="9">The EXKPK motif is conserved in inositol-pentakisphosphate 2-kinases of both family 1 and 2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IPK1 type 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.158" evidence="3 9"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556491.1">
        <property type="nucleotide sequence ID" value="XM_002556445.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3L8"/>
    <dbReference type="FunCoup" id="C5E3L8">
        <property type="interactions" value="49"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3L8"/>
    <dbReference type="GeneID" id="8294854"/>
    <dbReference type="KEGG" id="lth:KLTH0H14630g"/>
    <dbReference type="eggNOG" id="ENOG502S05I">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046294_0_0_1"/>
    <dbReference type="InParanoid" id="C5E3L8"/>
    <dbReference type="OMA" id="FIELRCK"/>
    <dbReference type="OrthoDB" id="272370at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035299">
        <property type="term" value="F:inositol-1,3,4,5,6-pentakisphosphate 2-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032958">
        <property type="term" value="P:inositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009286">
        <property type="entry name" value="Ins_P5_2-kin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14456">
        <property type="entry name" value="INOSITOL POLYPHOSPHATE KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14456:SF2">
        <property type="entry name" value="INOSITOL-PENTAKISPHOSPHATE 2-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06090">
        <property type="entry name" value="Ins_P5_2-kin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR30629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="288" mass="32820" checksum="3C78419229ABB09E" modified="2009-07-28" version="1">MVSTKSASCARLINSGNANAIIQLPGANYLIRCCVRFDSVDKNNKYTLENIEYINTQVRPLLGDCVVSLEPEFLKLDDLLLILRNFVPNCDTDEVVALKMEDLTRCIDGTVNVDHFTKIHSTRDHRTIVWEFKPKWLGNSESSHSLCRNCTHNKLKKRDIRYCYAMALKDPADVVSQCFSRQKVPEQFLRDLRAYLGSEESILVKLAAAQKKLAVVAPRPSELNSESDVTTELALLMTLRDVSCFIRWEASGAISAKIIDVDLKPSSKWRHWATEQRKVHTYCEPVYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3P5</accession>
    <name>C5E3P5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0H15268p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0H15268g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556518.1">
        <property type="nucleotide sequence ID" value="XM_002556472.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3P5"/>
    <dbReference type="FunCoup" id="C5E3P5">
        <property type="interactions" value="588"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3P5"/>
    <dbReference type="GeneID" id="8294883"/>
    <dbReference type="KEGG" id="lth:KLTH0H15268g"/>
    <dbReference type="eggNOG" id="KOG1441">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019048_4_1_1"/>
    <dbReference type="InParanoid" id="C5E3P5"/>
    <dbReference type="OMA" id="FWYTVSS"/>
    <dbReference type="OrthoDB" id="1588579at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004853">
        <property type="entry name" value="Sugar_P_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03151">
        <property type="entry name" value="TPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="119"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="240"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="272"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="330"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="Sugar phosphate transporter" evidence="7">
        <location>
            <begin position="84"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR30656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="448" mass="49854" checksum="21A778EED0E53D0F" modified="2009-07-28" version="1">MITTQSTTQSTTTKRRNSVHTNLFDPKLYQVPTLPQGYHKSPNSAPRSSIPEKPIAGNENLLQQKVDHLIPEQLRGYLPVVNARVTSLCCVWYITSSISSNLSKAILSTFPHPVALTELQFLISGALCLLFVSLANWLSSDKLKKSSASKTLLSFPEGILPSYLDGDFKKCVLRKFLTPCKLALTTTLPMGLFQLVGHISSHKATSLIPVSLVHSVKALSPIMTVCFYRFFKGKHYNRMTYLTLVPLICGVIITCWSTHGHKKPSESANEKTFVLGLFFALLSMGIFVSQNIFAKGILTVKKQRGILPSKAEKLSNMNEKEFSPFQIDKLTILFYCSCIGFILTLPFFISDGLINFNSDKSFLNDFCLKTLFLVLIHGATHFFQAMLAFQLIGMLSPVNYSIANIMKRIVIIAVALAWESSLSLRQVLGLSMTVVGLYGYDKWGLSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5DB98</accession>
    <name>C5DB98_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">KLTH0A00792p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0A00792g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PIGU family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551497.1">
        <property type="nucleotide sequence ID" value="XM_002551451.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DB98"/>
    <dbReference type="FunCoup" id="C5DB98">
        <property type="interactions" value="714"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DB98"/>
    <dbReference type="GeneID" id="8290288"/>
    <dbReference type="KEGG" id="lth:KLTH0A00792g"/>
    <dbReference type="eggNOG" id="KOG2552">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030193_0_1_1"/>
    <dbReference type="InParanoid" id="C5DB98"/>
    <dbReference type="OMA" id="ALWHLWI"/>
    <dbReference type="OrthoDB" id="549017at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042765">
        <property type="term" value="C:GPI-anchor transamidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016255">
        <property type="term" value="P:attachment of GPI anchor to protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009600">
        <property type="entry name" value="PIG-U"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13121">
        <property type="entry name" value="GPI TRANSAMIDASE COMPONENT PIG-U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13121:SF0">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL GLYCAN ANCHOR BIOSYNTHESIS CLASS U PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06728">
        <property type="entry name" value="PIG-U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950297" evidence="10">
        <location>
            <begin position="21"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="159"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="247"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="313"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="347"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR21055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="390" mass="43699" checksum="59124067168057DB" modified="2009-07-28" version="1">MNLRELQVVFACLASRFGAAAVFPLLQQQLDRSVEFSTPVTSFRSLQEGVFLLQNDFPLYSGGVMHHAPLLVALMSTFNSNTLISLLFTCADSAIAYQLIAMAKCFQNLHLPAWVPGALYVINPLVLLSSVSRSTVVFSNLAISTALLSALQGKAVLSAASIAVAAYLSLYPLLLLVPILTLLPKDRFKTVAITFTTLAGLLLSSYKINDENWNYLFSTYGVIITFSKIIPNLGLWWYFFVELFEMFIPFFKAVFNLFIVSCIIPFSLRFHKQPFYAFILCLCWITLTKSYPTLGEGGFLLSFVPFFRPLFGYFRYSVISTLLFIHVSVLSPVFYHLWVDLGSGNSNFFYAISLVYALGLATVLVDLVWAMLRIEYDKGKPNLNLKLTPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBD2</accession>
    <name>C5DBD2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0A01584p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0A01584g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551531.1">
        <property type="nucleotide sequence ID" value="XM_002551485.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBD2"/>
    <dbReference type="FunCoup" id="C5DBD2">
        <property type="interactions" value="271"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBD2"/>
    <dbReference type="GeneID" id="8290324"/>
    <dbReference type="KEGG" id="lth:KLTH0A01584g"/>
    <dbReference type="eggNOG" id="KOG1674">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018149_0_2_1"/>
    <dbReference type="InParanoid" id="C5DBD2"/>
    <dbReference type="OMA" id="HEEYIQH"/>
    <dbReference type="OrthoDB" id="10250320at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20557">
        <property type="entry name" value="CYCLIN_ScPCL1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013922">
        <property type="entry name" value="Cyclin_PHO80-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012104">
        <property type="entry name" value="PHO85_cyclin_1/2/9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615:SF114">
        <property type="entry name" value="PHO85 CYCLIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016511">
        <property type="entry name" value="Cyclin_Pcl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin N-terminal" evidence="1">
        <location>
            <begin position="81"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="281" mass="31273" checksum="15616DFAA5F67426" modified="2009-07-28" version="1">MRRCGMWAGGACGGQSTLSITASNENSVKMSDTKVLKILFKSPVTDDMIRYLTNTTLSVLPCAREPRQAYPSPPSSPGAASKRALPSLMTFITRLVRYTNVYTSTLLTATCYLEKLRRLLPRDAHGLPSTGHRIFLACLILSAKYHNDSSPQNKHWAAYTDGLFCLDDVNLMERQLLQLLDWDLRVEEQELCDALEPLLEPIRHDLARSRKAMARNYYHQRNMSTCSSTSTLVSTPSLPSLASMKSLTAMKSLPSLPRLPSKASLYAPLYSQCDYSSATLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBI2</accession>
    <name>C5DBI2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0A02838p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0A02838g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551581.1">
        <property type="nucleotide sequence ID" value="XM_002551535.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBI2"/>
    <dbReference type="STRING" id="559295.C5DBI2"/>
    <dbReference type="GeneID" id="8290380"/>
    <dbReference type="KEGG" id="lth:KLTH0A02838g"/>
    <dbReference type="HOGENOM" id="CLU_943562_0_0_1"/>
    <dbReference type="InParanoid" id="C5DBI2"/>
    <dbReference type="OrthoDB" id="4036276at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="295" mass="32135" checksum="44B6EC6CA8A8E445" modified="2009-07-28" version="1">MHEIPKGPREPGEPAEPTEPVEPGALGEHAGHALRERDALRLRVRALEEQLGSQSATEPQAPSPAGHAQELLHLLARDASASAPASLPASHRFHALPLLDPGSRLSCLQEFLPLLQVRSLGPPATVVSFGTQLQLTLEWATQSARVTSLRVLEVSPPQAKLALRPLVTHCERALNVSYLLLGCYEYSRLHAARSDLWQEIARASRRWLVQQRSESCLRVSSLPAPAAAAATAPSLAVFVHYRIDFESVPLPQSLLSVELFNGPSTVPHANHVCNALIQEYGLRHGLQEFLRAVLS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-11-03" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBT1</accession>
    <name>GET2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Golgi to ER traffic protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">GET2</name>
        <name type="ordered locus">KLTH0A05126g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Together with GET1, acts as a membrane receptor for soluble GET3, which recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. The GET complex cooperates with the HDEL receptor ERD2 to mediate the ATP-dependent retrieval of resident ER proteins that contain a C-terminal H-D-E-L retention signal from the Golgi to the ER.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the Golgi to ER traffic (GET) complex, which is composed of GET1, GET2 and GET3. Within the complex, GET1 and GET2 form a heterotetramer which is stabilized by phosphatidylinositol binding and which binds to the GET3 homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GET2 family.</text>
    </comment>
    <comment type="sequence caution" evidence="3">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="CAR21238" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21238.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551680.1">
        <property type="nucleotide sequence ID" value="XM_002551634.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBT1"/>
    <dbReference type="SMR" id="C5DBT1"/>
    <dbReference type="FunCoup" id="C5DBT1">
        <property type="interactions" value="69"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBT1"/>
    <dbReference type="GeneID" id="8290484"/>
    <dbReference type="KEGG" id="lth:KLTH0A05126g"/>
    <dbReference type="eggNOG" id="ENOG502QW0H">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066477_0_0_1"/>
    <dbReference type="InParanoid" id="C5DBT1"/>
    <dbReference type="OrthoDB" id="4097053at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043529">
        <property type="term" value="C:GET complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045048">
        <property type="term" value="P:protein insertion into ER membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03114">
        <property type="entry name" value="Get2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014802">
        <property type="entry name" value="GET2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028143">
        <property type="entry name" value="Get2/sif1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28263">
        <property type="entry name" value="GOLGI TO ER TRAFFIC PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28263:SF1">
        <property type="entry name" value="GOLGI TO ER TRAFFIC PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08690">
        <property type="entry name" value="GET2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword id="KW-0931">ER-Golgi transport</keyword>
    <keyword id="KW-0333">Golgi apparatus</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000388633" description="Golgi to ER traffic protein 2">
        <location>
            <begin position="1"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="1">
        <location>
            <begin position="1"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="topological domain" description="Lumenal" evidence="1">
        <location>
            <begin position="194"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="215"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="1">
        <location>
            <begin position="235"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="282"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="topological domain" description="Lumenal" evidence="1">
        <location>
            <begin position="303"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="34"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="68"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="78"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="91"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="306" mass="33900" checksum="87C836D26F67EF6B" modified="2009-11-03" version="2">MSEISDAEKRRILREKRQQKFNKGGASSRLAKITGQTENSFLSTESPLDSRESTYPAQETKAPAGNEDSTKQMDELLAKATSKTTSKASSPPGSAEQQNGNPELDLFAQIAKLQQNANNETVSTDPSGTPDIFAQLMASMQQDEAKGGSPGATAQQPIDPAIVEAHNIAVNKLKSYTILVKWLFFLLPYLYYITHSARDPFQHNAVNYVLDRSNFFTVFTTFEIVALSVYYQLLMSAEKSHNVNTLDNNSKILKLVSMVPPGLVPIPNLRGKVAQALQYWDVVSMYLTDLCFAIVLAGLFQYYHSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBV8</accession>
    <name>C5DBV8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Autophagy-related protein 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 11">Autophagy-related E2-like conjugation enzyme ATG3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0A05720g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt) and autophagy. Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8. The ATG12-ATG5 conjugate plays a role of an E3 and promotes the transfer of ATG8 from ATG3 to phosphatidylethanolamine (PE). This step is required for the membrane association of ATG8. The formation of the ATG8-phosphatidylethanolamine conjugate is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). The ATG8-PE conjugate mediates tethering between adjacent membranes and stimulates membrane hemifusion, leading to expansion of the autophagosomal membrane during autophagy.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATG3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551707.1">
        <property type="nucleotide sequence ID" value="XM_002551661.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBV8"/>
    <dbReference type="FunCoup" id="C5DBV8">
        <property type="interactions" value="1128"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBV8"/>
    <dbReference type="GeneID" id="8290511"/>
    <dbReference type="KEGG" id="lth:KLTH0A05720g"/>
    <dbReference type="eggNOG" id="KOG2981">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027518_2_0_1"/>
    <dbReference type="InParanoid" id="C5DBV8"/>
    <dbReference type="OMA" id="HCPTWSW"/>
    <dbReference type="OrthoDB" id="1584384at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019776">
        <property type="term" value="F:Atg8-family ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061723">
        <property type="term" value="P:glycophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044804">
        <property type="term" value="P:nucleophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1460.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007135">
        <property type="entry name" value="Atg3/Atg10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12866">
        <property type="entry name" value="UBIQUITIN-LIKE-CONJUGATING ENZYME ATG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12866:SF2">
        <property type="entry name" value="UBIQUITIN-LIKE-CONJUGATING ENZYME ATG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03987">
        <property type="entry name" value="Autophagy_act_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0072">Autophagy</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="231"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="233"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR21265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="294" mass="33655" checksum="328807165BDC6E97" modified="2009-07-28" version="1">MIRSKLASWREYLTPVTHTSTFQSSGQITPEEFTQAGDYLCHMFPTWEWNSTSKDVSYRDFLPKEKQFLVTRKVPCSARAEQLTTVEGAEEGDNADGWVVEGQVKTRSTEEHANADIEDMLEGMDLEEVEQDDDDMVAIAPNDRRFYDLYITYSTSYRVPKIYLVGFNGDGSPLTPEQMFEDISPDYRTKTATIERLPFQHTHVTSVSIHPCKHANVMRVLMERIREVKQRKNASLANTTNPSSGRGKEGDEDWEDIQNDVEGGLRVDQYLIVFLKFVSSVTPGIEHDYTMEGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DC84</accession>
    <name>C5DC84_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0B00946p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0B00946g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551833.1">
        <property type="nucleotide sequence ID" value="XM_002551787.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DC84"/>
    <dbReference type="FunCoup" id="C5DC84">
        <property type="interactions" value="1432"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DC84"/>
    <dbReference type="GeneID" id="8290659"/>
    <dbReference type="KEGG" id="lth:KLTH0B00946g"/>
    <dbReference type="eggNOG" id="KOG1889">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003016_7_4_1"/>
    <dbReference type="InParanoid" id="C5DC84"/>
    <dbReference type="OMA" id="ITKAQPV"/>
    <dbReference type="OrthoDB" id="405996at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034593">
        <property type="term" value="F:phosphatidylinositol bisphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043812">
        <property type="term" value="F:phosphatidylinositol-4-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002013">
        <property type="entry name" value="SAC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45662">
        <property type="entry name" value="PHOSPHATIDYLINOSITIDE PHOSPHATASE SAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45662:SF2">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-3-PHOSPHATASE SAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02383">
        <property type="entry name" value="Syja_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50275">
        <property type="entry name" value="SAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="522"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="555"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="domain" description="SAC" evidence="2">
        <location>
            <begin position="115"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR21395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="623" mass="70945" checksum="CAB4F5CD7662C660" modified="2009-07-28" version="1">MAGPLLYSHLGEKIVFKLATPSSEKDVVLSLGPSGQDISLESPEIFPTRGKIVKSAALIGCIKLKLNSYAIFAHRVEEFGVLAGHRIFKVVEHTILPLNKGGQRDKDEQQYLDLLEMQLSSATLFYSYTYDLTNSAQRNEKLDGSSWKTADTRFFWNHYVTHSLRTLAEKCPAAGDFIQPMIFGYAHFIETALNNVPITIGLVTRRSRFRAGTRYFRRGIDSDGNVANFNETEQFLLVKKPEGGNSEFFSFLQTRGSVPVYWGEINNLKYKPNLVLGENSSLDATAKHFDQQKSLYGENYLVNLVNQKGHELPVKNAYESAVNALNDPMIHYVYFDFHHECRKMKWYRVKLLIDHLQKMGLDPEDTFHKIVSPDGKTVDVVREQKSVVRTNCMDCLDRTNVVQSVLASWVLQHEFQIANVVPEGTLWELDLKLLAKFQNFWADNADAVSFSYSGTGALKTDFTRTGKRTRKGAFADFMNSVSRYYQNNLSDGPRQDSFDLFHGNFLPFENSFQSPFPDRRPLSIQIVPTVICAAITVIAATVFFPKGHFFSTKNLSFFFVSSAIVILSLRFIFRNGEQYVNWPKFNNLGFLTAVQTHNKERTFKGIRYVQSPKFSKPGELKRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCB7</accession>
    <name>C5DCB7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ribosomal RNA-processing protein 41</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0B01738g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="6">Component of the RNA exosome complex. Specifically part of the catalytically inactive RNA exosome core complex (Exo-9) which may associate with the catalytic subunits RRP6 and DIS3 in cytoplasmic- and nuclear-specific RNA exosome complex forms. Exo-9 is formed by a hexameric base ring of RNase PH domain-containing subunits and a cap ring consisting of CSL4, RRP4 and RRP40.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase PH family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551866.1">
        <property type="nucleotide sequence ID" value="XM_002551820.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCB7"/>
    <dbReference type="FunCoup" id="C5DCB7">
        <property type="interactions" value="923"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCB7"/>
    <dbReference type="GeneID" id="8290694"/>
    <dbReference type="KEGG" id="lth:KLTH0B01738g"/>
    <dbReference type="eggNOG" id="KOG1068">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063514_0_1_1"/>
    <dbReference type="InParanoid" id="C5DCB7"/>
    <dbReference type="OMA" id="ECRINTH"/>
    <dbReference type="OrthoDB" id="437922at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071028">
        <property type="term" value="P:nuclear mRNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071051">
        <property type="term" value="P:poly(A)-dependent snoRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11370">
        <property type="entry name" value="RNase_PH_RRP41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.70:FF:000004">
        <property type="entry name" value="Exosome complex component Rrp41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015847">
        <property type="entry name" value="ExoRNase_PH_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050080">
        <property type="entry name" value="RNase_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953:SF0">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03725">
        <property type="entry name" value="RNase_PH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="Ribonuclease PH domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0271">Exosome</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="8">
        <location>
            <begin position="21"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="9">
        <location>
            <begin position="156"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00063066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00077929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR21428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="245" mass="27432" checksum="F71E89E76DDA21BE" modified="2009-07-28" version="1">MSRLEIYSPEGLRVDGRRWNELRRLECSINTHANASDGSSYLEQGNNKVITLVTGPQEPKLRSQMNVTKATISVALNITRFSKIERSKSSHKNERRVLEMQTALVRTFEKNVMLHLYPRTQIEIQIHVLQQDGGLMSSLINGITLALIDAGIAMYDYISGVSIGLYDTTPLLDLNSVEENAMSSVTLGVVGKSEKLSLLLVEDKIPLDRMESVLAIGIAGSHRIRELMDKELRKHGERRLANMST</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCF1</accession>
    <name>C5DCF1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0B02530p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0B02530g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in ribosomal function.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551900.1">
        <property type="nucleotide sequence ID" value="XM_002551854.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCF1"/>
    <dbReference type="FunCoup" id="C5DCF1">
        <property type="interactions" value="1128"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCF1"/>
    <dbReference type="GeneID" id="8290730"/>
    <dbReference type="KEGG" id="lth:KLTH0B02530g"/>
    <dbReference type="eggNOG" id="KOG1486">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044997_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCF1"/>
    <dbReference type="OMA" id="DVCDQVH"/>
    <dbReference type="OrthoDB" id="603at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903833">
        <property type="term" value="P:positive regulation of cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01896">
        <property type="entry name" value="DRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001436">
        <property type="entry name" value="Developmentally-regulated GTP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000003">
        <property type="entry name" value="Developmentally-regulated GTP-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1070">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045001">
        <property type="entry name" value="DRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031662">
        <property type="entry name" value="GTP-binding_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006074">
        <property type="entry name" value="GTP1-OBG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43127">
        <property type="entry name" value="DEVELOPMENTALLY-REGULATED GTP-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16897">
        <property type="entry name" value="MMR_HSR1_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="TGS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00905">
        <property type="entry name" value="GTP1_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="4">
        <location>
            <begin position="64"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="TGS" evidence="5">
        <location>
            <begin position="292"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00059727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR21462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="368" mass="40909" checksum="F7E88B5858E7193D" modified="2009-07-28" version="1">MGVIEKIKAIEEEMARTQKNKATEYHLGLLKGKLARFRQQLLAEETAPTGGGGTGFEVAKSGDARVVLIGYPSVGKSSLLGKVTTTKSEIAHYAFTTLTSVPGVLKYQGAEVQIVDLPGIIYGASQGKGRGRQVVATARTADLILMVLDATKGAHQRESLEKELEAVGIRLNKEKPNIYYKKKETGGVKVTFTSPSRANLTEEAIKMILKDYRVHNAEVLVRDDKCTIDDFIDIINDQHCNYIKCLYAYNKIDAVSLEEVDRLAREPNTVVMSCENDLGLDDVVDEIWYQLNLSRVYTKKRGVKPDFSDPLVVRNASTIGDMCHSIHRDFKEKFKYALVWGSSAKHSPQKCGLTHKLHDEDVVSLFTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCG1</accession>
    <name>C5DCG1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0B02772p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0B02772g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551910.1">
        <property type="nucleotide sequence ID" value="XM_002551864.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCG1"/>
    <dbReference type="GeneID" id="8290741"/>
    <dbReference type="KEGG" id="lth:KLTH0B02772g"/>
    <dbReference type="eggNOG" id="ENOG502QR5Q">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003690_4_2_1"/>
    <dbReference type="InParanoid" id="C5DCG1"/>
    <dbReference type="OMA" id="YTSLYFM"/>
    <dbReference type="OrthoDB" id="449263at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000224">
        <property type="term" value="F:peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006516">
        <property type="term" value="P:glycoprotein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.60:FF:000002">
        <property type="entry name" value="Glycosyl hydrolase family 92"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.60">
        <property type="entry name" value="alpha-1,2-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1610.10">
        <property type="entry name" value="alpha-1,2-mannosidases domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2080.10">
        <property type="entry name" value="GH92 mannosidase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014718">
        <property type="entry name" value="GH-type_carb-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005887">
        <property type="entry name" value="GH92_a_mannosidase_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041371">
        <property type="entry name" value="GH92_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012939">
        <property type="entry name" value="Glyco_hydro_92"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050883">
        <property type="entry name" value="PNGase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01180">
        <property type="entry name" value="aman2_put"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12143:SF38">
        <property type="entry name" value="ALPHA-1,2-MANNOSIDASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_5G10520)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12143">
        <property type="entry name" value="PEPTIDE N-GLYCANASE PNGASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07971">
        <property type="entry name" value="Glyco_hydro_92"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17678">
        <property type="entry name" value="Glyco_hydro_92N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950417" evidence="2">
        <location>
            <begin position="20"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 92 N-terminal" evidence="4">
        <location>
            <begin position="81"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 92" evidence="3">
        <location>
            <begin position="340"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="842"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="850"/>
            <end position="863"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF17678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR21472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="863" mass="97734" checksum="004CA33BCDD2BE8C" modified="2009-07-28" version="1">MNVLLSFLALSKFFLLAIAVPVRDDDLYTEMVEAQLDEEFEEQVERTEVRSSGFESSPGATGRALFGLSDGLFEEFPFLNYVDVFYGTEGGGHMFPGTTVPFGMCKMGVDVLHPHSTDAYSGYHSTGDVCGISMLHESGTGGSPTYGVVSQLPLMASSASDVDTSRQIAFPRSKPDAGHIGYYRVSLSNRVTIEFSSNSRSGLYKYTFPTKHQQHHMSPIVMVNVTQHLHSVKRPWWTQNFEGGYIKVDKSYKSYRGKAYFSGGWSDPGTWSVSFYGVFDKPAKRVRAFHGSKAYNRLRLNLAKSLNSNMGMLFEFEKGTKVLYSHVGVSFSETDGINVARQNLALNYPEENMFDLHWSVRSTAEAWDNEVFSKVNIDESKEDPIIVEKMYNALYGSHLMPSDKSGREAPWPTNEPYYDDWFTLWDTFRCLHPLLNILNTKRSADMTRSLIEIWRKEGYMPDGRSASRSGRTQGGSNADIVLADAFVKGVGEGVDWQDGFSAMRSNADKSPEYILDKAAPDSTNKYGRGALNDWLELGYITRRFSRSVTRTMEYAYNDFALSVVAEGLGYFELAKQYLKRSSNWQNIWNFEATTPEYDYSGFIQPKDSNGNFIEKKYNPLSCFGCYWRDDEYEGKPIEYGWAVPHDIQSLKEFIGPDELFMRRLDDMFDLYGDGIADIGNEPSFLTPYLFNFINAQYRTSETLGYLIDHKFLLGPKGLPGNSDAGAMQAWLWFGLVGFYPIAGTDIYLITSPKITELEIQLEGNGKALIQAHNLYAEDADSEYDYDKAHSSFTRNLYISSVELNGKLINRNWFTHEELFGSNGGLLVFTMTDEPTFWDADGEYPPSRGHFQRDDIERPNRLVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCG6</accession>
    <name>C5DCG6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0B02904p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0B02904g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551915.1">
        <property type="nucleotide sequence ID" value="XM_002551869.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCG6"/>
    <dbReference type="GeneID" id="8290747"/>
    <dbReference type="KEGG" id="lth:KLTH0B02904g"/>
    <dbReference type="HOGENOM" id="CLU_1240337_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCG6"/>
    <dbReference type="OMA" id="LEYANPW"/>
    <dbReference type="OrthoDB" id="4035186at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="205" mass="23054" checksum="1816CD664B9AC82B" modified="2009-07-28" version="1">MTFRLIMVDPSPSSKEHLDFSTEKPESVKQHDARGSSKRRKDLSVVMHTFEKGRVEKVSPPCRGICTKKKNHKNAAAAKPSEKRCKKLEGVQDEEKMNSSCVIPPKPLTFVTSVASDYSLEYANAWQSSLEFNSKKAQRPLFRITKYKGRKGCEKVESSTELLGTIDPNFCFSYNSLNSSKEKGAKTPPMNFSTAPVFKVLLNDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCK9</accession>
    <name>C5DCK9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0B03894p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0B03894g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551958.1">
        <property type="nucleotide sequence ID" value="XM_002551912.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCK9"/>
    <dbReference type="GeneID" id="8290792"/>
    <dbReference type="KEGG" id="lth:KLTH0B03894g"/>
    <dbReference type="eggNOG" id="KOG0255">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008455_11_5_1"/>
    <dbReference type="InParanoid" id="C5DCK9"/>
    <dbReference type="OMA" id="PMMLSCW"/>
    <dbReference type="OrthoDB" id="6770063at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17323">
        <property type="entry name" value="MFS_Tpo1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000011">
        <property type="entry name" value="MFS multidrug transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF48">
        <property type="entry name" value="MULTIDRUG TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_5G02700)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="162"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="202"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="227"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="260"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="290"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="354"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="404"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="445"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="472"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="516"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="540"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="136"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="576"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="40"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="82"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="595"/>
            <end position="606"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR21520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="606" mass="67896" checksum="F3C116EC1628DA5E" modified="2009-07-28" version="1">MASISSSELESIQSHAQEYTPHQEKVSGDGATGAQTHRMMSKESDEAELRKLLTAHSRGVQKRLSRLESNTHGIGPVEPPLDLERRETRPAPDSTFHEQDKWKYPIDEETGMRIVEFIDDDSEDPRNWPTRQKWNYTVLLGIICFDVAMMSAIITGDMDSPAAYFGVSMEVMCLCVSLMVWGFGLGPMLFAPLSEEFGRNPVYLVTLFIAVVFIVPCGAAKNIGTLLAFRFLDGLAFSAPMCLIGGSLSDMWRNKERGVAMSLFSAAPFLGPVMGPIVGSLLSVKCSWRWVYWFMVIFSGCLYAVMAVFLPETHHQTLLKKRAKKLRKLTGDPSYRAICEIKVRSMKEVAQETLFRPLVLLTELIVFLITMYMSVIYGLLYMFFFAYPMVYTEGKHYGAIKTSIMFIPVGAGVIGSTVAAPFLNKDYVKRAQRYLDRGEVPPAELRLIPMMIGCWFVPAGLFAYAWSSYPRLSWAGPCFSGFACGFGFNMLYNPANNYIVDSYQHYAASGLAAKTFVRSMWGGAVPLFTIQMYRRLNYEWASTLMAFISLACCAIPYLFYFYGARIRQKSKYAYSPSVDTTNTTRTDIENQAAGSHSSNSEGSSKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCL2</accession>
    <name>C5DCL2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0B03982p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0B03982g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551961.1">
        <property type="nucleotide sequence ID" value="XM_002551915.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCL2"/>
    <dbReference type="GeneID" id="8290796"/>
    <dbReference type="KEGG" id="lth:KLTH0B03982g"/>
    <dbReference type="HOGENOM" id="CLU_1299920_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCL2"/>
    <dbReference type="OrthoDB" id="10404390at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="162"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="212" mass="22487" checksum="C75C6FA84F0DC2CA" modified="2009-07-28" version="1">MPCSFILYVPYTPCVRALRAPYTSRVLSDALLSELSGSSDVRPWLRSRLNTCSISVSLYIHLMPAYPTTPSAPNPARYPKNCEGGVSALCSWLALCDMLVRRLTAILQEAGLIMWCAGVLVCYCALAGSAGPGRYVRAASPPLYTAATGLCAPTPVPRDAEQSALAGSNAGGRARRNLKAAPQTGRQGRHNGAIYSGSIHKGYVETGTDAHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCW1</accession>
    <name>C5DCW1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0B06248p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0B06248g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552060.1">
        <property type="nucleotide sequence ID" value="XM_002552014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCW1"/>
    <dbReference type="GeneID" id="8290899"/>
    <dbReference type="KEGG" id="lth:KLTH0B06248g"/>
    <dbReference type="HOGENOM" id="CLU_1518131_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCW1"/>
    <dbReference type="OrthoDB" id="4035669at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="44"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="177" mass="19051" checksum="EC5F0568D9E23117" modified="2009-07-28" version="1">MTSFGTYRTMEDPELLFQFSTPLVGSSRTLPTGISAGEPSSRWPGFDASPRAAAADDEERDGDAATLQGSGASICDEAATLYSHTVKRDTPAESGARSEAPWVNAAAIGSGYAPHPLKSGTASVYRSNAALLSRFNNALSKSFSSDQLHQIERMVDEFWLRESALTEDMFEDSSDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDF8</accession>
    <name>C5DDF8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein farnesyltransferase/geranylgeranyltransferase type-1 subunit alpha</fullName>
            <ecNumber evidence="4">2.5.1.58</ecNumber>
            <ecNumber evidence="3">2.5.1.59</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">CAAX farnesyltransferase subunit alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">FTase-alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Ras proteins prenyltransferase subunit alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Type I protein geranyl-geranyltransferase subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0C00660g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein prenyltransferase subunit alpha family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.58" evidence="4"/>
    <dbReference type="EC" id="2.5.1.59" evidence="3"/>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552257.1">
        <property type="nucleotide sequence ID" value="XM_002552211.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDF8"/>
    <dbReference type="FunCoup" id="C5DDF8">
        <property type="interactions" value="191"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDF8"/>
    <dbReference type="GeneID" id="8291115"/>
    <dbReference type="KEGG" id="lth:KLTH0C00660g"/>
    <dbReference type="eggNOG" id="KOG0530">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026582_1_0_1"/>
    <dbReference type="InParanoid" id="C5DDF8"/>
    <dbReference type="OMA" id="WAIRTFN"/>
    <dbReference type="OrthoDB" id="272289at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005953">
        <property type="term" value="C:CAAX-protein geranylgeranyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005965">
        <property type="term" value="C:protein farnesyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004662">
        <property type="term" value="F:CAAX-protein geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004660">
        <property type="term" value="F:protein farnesyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.120">
        <property type="entry name" value="Protein prenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002088">
        <property type="entry name" value="Prenyl_trans_a"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11129">
        <property type="entry name" value="PROTEIN FARNESYLTRANSFERASE ALPHA SUBUNIT/RAB GERANYLGERANYL TRANSFERASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11129:SF1">
        <property type="entry name" value="PROTEIN FARNESYLTRANSFERASE_GERANYLGERANYLTRANSFERASE TYPE-1 SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01239">
        <property type="entry name" value="PPTA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48439">
        <property type="entry name" value="Protein prenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51147">
        <property type="entry name" value="PFTA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0637">Prenyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00042436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00043086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00043219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR21819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="315" mass="36450" checksum="9157C1DC8950DCB5" modified="2009-07-28" version="1">MSDAPEFQYDDIEPLPISGGQEGELCQIMYDGEYRTVMGIARALLQKLEFSERALHATSQAIDLVPAFYTMWNYRFQIVKALYGTDGAELNRELDWLDEFTLNNPKNYQIWSYRQALLRLHPSPEFQRELPILQSMIDDDTKNYHVWSYRRWCVLFFGDFTNELQFASSLIARDVYNNSAWCHRMFVLKNQVPKGPPAEALTGELHYTKKQIELAPQNISSWNYLRGLYDEFLDGKADPAIMEFALSFTPGIFAADASDTGPQIQSSYALEFLARAYAKNEDTFAQARKAYQALSDHFDPIRKNFWAYKIRSLTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDJ8</accession>
    <name>C5DDJ8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MICOS complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0C01562g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the mitochondrial contact site and cristae organizing system (MICOS) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552297.1">
        <property type="nucleotide sequence ID" value="XM_002552251.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDJ8"/>
    <dbReference type="FunCoup" id="C5DDJ8">
        <property type="interactions" value="46"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDJ8"/>
    <dbReference type="GeneID" id="8291155"/>
    <dbReference type="KEGG" id="lth:KLTH0C01562g"/>
    <dbReference type="eggNOG" id="ENOG502S70K">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086433_1_0_1"/>
    <dbReference type="InParanoid" id="C5DDJ8"/>
    <dbReference type="OMA" id="QMIFNES"/>
    <dbReference type="OrthoDB" id="2399148at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061617">
        <property type="term" value="C:MICOS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044284">
        <property type="term" value="C:mitochondrial crista junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042407">
        <property type="term" value="P:cristae formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019166">
        <property type="entry name" value="MIC26/MIC27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033181">
        <property type="entry name" value="Mic26_fungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28268">
        <property type="entry name" value="MICOS SUBUNIT MIC26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28268:SF1">
        <property type="entry name" value="MICOS SUBUNIT MIC26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09769">
        <property type="entry name" value="ApoO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="1" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="230" mass="25274" checksum="9C91CBF5EAB2F25A" modified="2009-07-28" version="1">MAPNFYRPVELVSEGVVPPETGSIVSSEAKEAAPSHVTKQSVSHSATEILGDNKLLDGISVRSPTYLNKFFSSWRLNLSGLIENTCDTIDEKSRKYYSYERKLTTTVANLHSDPRELLLPGMTYSLVAAMSGSVLTRNRNILARIMAPLALGACCFSYVLPVTSSNTFGLLFDLEKQAFPRFAQNQRALYNTCKNTVHQTIAVAGRTSDAISGSFSKSMHLVKEWTGLNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDK9</accession>
    <name>C5DDK9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0C01826p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0C01826g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552308.1">
        <property type="nucleotide sequence ID" value="XM_002552262.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDK9"/>
    <dbReference type="FunCoup" id="C5DDK9">
        <property type="interactions" value="33"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDK9"/>
    <dbReference type="GeneID" id="8291167"/>
    <dbReference type="KEGG" id="lth:KLTH0C01826g"/>
    <dbReference type="eggNOG" id="ENOG502QUW8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034954_0_0_1"/>
    <dbReference type="InParanoid" id="C5DDK9"/>
    <dbReference type="OMA" id="WRLIFIQ"/>
    <dbReference type="OrthoDB" id="74314at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052816">
        <property type="entry name" value="Peroxisomal_Membrane_PEX28-32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010482">
        <property type="entry name" value="TECPR1-like_DysF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28304:SF1">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28304">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06398">
        <property type="entry name" value="Pex24p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="214"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="350"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="TECPR1-like DysF" evidence="8">
        <location>
            <begin position="174"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="107"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="116"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF06398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR21870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="542" mass="62244" checksum="0D32E8202CC1E52F" modified="2009-07-28" version="1">MTEMPVTRGRRDSIIGGFMARKYANKNHAVPEEDDDSTFKRVSNRDLIHGVASSFFEAAYQRLKSSKASDGITIDEDYDQFAQFVPSESSDGFWKDENFRQEYELGSELGSSQTSEPEKMAEKPKSERTRGTQEHFIDILLDKMISTILPENFPEREHFSQRLNDPDRKRRQKLSATILASNLKILTTKLGSIFELQDSVIRLVAWRNPSGTITMLIMVTMICFNPIYLAVIPLLYVLYGLMVPGYMHRHPPRRTNFLSRKQYGRSLIVSLTSGGKRTHNYLNEDIREYDYNLEADPQDVKKAHNIKQSMEFVVNLRDLQNSMSAMVSLSNSIERFVYGTAGFKDEHRSTVLFLAGLGALLLSGIISPFINWSALLAALAWLSMVAIHPKVRPKLAQVVKKEQLDRGKEALKRTERYDVILDEQPEVRVIEIFEIFKKEALSNNWSFVKYSSSVFDPRDSFRKAQVLPPGVDELECVCPPLTWSFDANSVWEIDYNVGGWSTERCLNLQIDDQFLVDDAFKRRRLTRKVLRYASPARKPSYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDS2</accession>
    <name>C5DDS2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Crh-like protein</fullName>
            <ecNumber evidence="14">3.2.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ordered locus">KLTH0C03344g</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1-&gt;4)-beta-linkages in chitin and chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.14"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the glycosyl hydrolase 16 family. CRH1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.2.-.-" evidence="14"/>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552371.1">
        <property type="nucleotide sequence ID" value="XM_002552325.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDS2"/>
    <dbReference type="FunCoup" id="C5DDS2">
        <property type="interactions" value="440"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDS2"/>
    <dbReference type="GeneID" id="8291235"/>
    <dbReference type="KEGG" id="lth:KLTH0C03344g"/>
    <dbReference type="eggNOG" id="ENOG502QVQI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040459_0_0_1"/>
    <dbReference type="InParanoid" id="C5DDS2"/>
    <dbReference type="OMA" id="WNATANQ"/>
    <dbReference type="OrthoDB" id="4781at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008843">
        <property type="term" value="F:endochitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06923">
        <property type="entry name" value="ChtBD1_GH16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000159">
        <property type="entry name" value="Glycosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017168">
        <property type="entry name" value="CHR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050546">
        <property type="entry name" value="Glycosyl_Hydrlase_16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963:SF22">
        <property type="entry name" value="GLYCOSIDASE CRH2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963">
        <property type="entry name" value="GLYCOSYL HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00722">
        <property type="entry name" value="Glyco_hydro_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037299">
        <property type="entry name" value="Glycosidase_CRH1_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="7 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 17" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002947794" description="Crh-like protein" evidence="17">
        <location>
            <begin position="22"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="18">
        <location>
            <begin position="67"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="343"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="415"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="356"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="15">
        <location>
            <position position="170"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="15">
        <location>
            <position position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR037299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR037299-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAR21933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="467" mass="49866" checksum="50A61747606CA193" modified="2009-07-28" version="1">MAGSKLFFLSAISALTSVVAAATTEKVVYCNETLAACPEDKPCCSQYGVCGSGSYCLGGCNPRYSHNITACMPMPVCKDFQTVFSNYSGDLQSQYTYLGDAEKHDWIYEGYTMDYDKEDALVLAMPKNSGGTVLSSTRYVWYGKVGARLKTSHDAGVVTAFIMFSSVQDEIDYEYVGNNLTQVQTNYYFEGVQNWTHSTNVSTTDTFDNYHLYEVDWHEDHIDWLIDGEVSRTLLKNSTFNATSGEYMFPQTPARIQISIWPGGNATNPVGTRSWAGGEINWDSSDIQDYGYYYALLQGVNVTCYDPPAGTVKNGSVSYEYISSENFTQNYVAITNGTTVMGSADDTGYNPEDGKSSSSSSSSSSSSPAASKSTQKGSSSTDKSSSTESGLKVATGSSSVSSAKQSGFVQNINSASSSATGKSSATSTGSSATSTGGAAGLSYAPISNNGLMHIAMGLFGAAFAFLY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2010-05-18" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDS4</accession>
    <name>ENOPH_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Enolase-phosphatase E1</fullName>
            <ecNumber evidence="1">3.1.3.77</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">2,3-diketo-5-methylthio-1-phosphopentane phosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">UTR4</name>
        <name type="ordered locus">KLTH0C03388g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-methylsulfanyl-2,3-dioxopentyl phosphate + H2O = 1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:49252"/>
            <dbReference type="ChEBI" id="CHEBI:58828"/>
            <dbReference type="EC" id="3.1.3.77"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 3/6.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 4/6.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HAD-like hydrolase superfamily. MasA/MtnC family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="CAR21935" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.77" evidence="1"/>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21935.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552373.1">
        <property type="nucleotide sequence ID" value="XM_002552327.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDS4"/>
    <dbReference type="SMR" id="C5DDS4"/>
    <dbReference type="FunCoup" id="C5DDS4">
        <property type="interactions" value="685"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDS4"/>
    <dbReference type="GeneID" id="8291237"/>
    <dbReference type="KEGG" id="lth:KLTH0C03388g"/>
    <dbReference type="eggNOG" id="KOG2630">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023273_1_1_1"/>
    <dbReference type="InParanoid" id="C5DDS4"/>
    <dbReference type="OrthoDB" id="272500at2759"/>
    <dbReference type="UniPathway" id="UPA00904">
        <property type="reaction ID" value="UER00876"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00904">
        <property type="reaction ID" value="UER00877"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043874">
        <property type="term" value="F:acireductone synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01629">
        <property type="entry name" value="HAD_EP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03117">
        <property type="entry name" value="Salvage_MtnC_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023943">
        <property type="entry name" value="Enolase-ppase_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027511">
        <property type="entry name" value="ENOPH1_eukaryotes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01691">
        <property type="entry name" value="enolase-ppase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20371">
        <property type="entry name" value="ENOLASE-PHOSPHATASE E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20371:SF1">
        <property type="entry name" value="ENOLASE-PHOSPHATASE E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01133">
        <property type="entry name" value="C1.5.4:_Enolase-phosphatase_Li"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0486">Methionine biosynthesis</keyword>
    <keyword id="KW-0539">Nucleus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000394001" description="Enolase-phosphatase E1">
        <location>
            <begin position="1"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="112"/>
            <end position="113"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="217" mass="24241" checksum="D7C8F3E1E9CCD170" modified="2010-05-18" version="2">MAYAAVLLDIEGTVCPISFVKESLFPFFLKQVDTLCSSQDLQVQRLLTQFQVDDVAGHIRSLVARDVKDPILKQLQGLVWENGYKDGQIKAPVYDDAIKFIKNSDVPVYIYSSGSVKAQKLLFQYVESRSNTLDLRPFIKGYFDINTSGVKTQSESYARIANNVGIKPNGVLFISDNPFELDAAKITGMQTMLAVRPGNSEVRDALKYNPVTNFDDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDW7</accession>
    <name>C5DDW7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">KLTH0C04378p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0C04378g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat HIR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552416.1">
        <property type="nucleotide sequence ID" value="XM_002552370.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDW7"/>
    <dbReference type="FunCoup" id="C5DDW7">
        <property type="interactions" value="885"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDW7"/>
    <dbReference type="GeneID" id="8291281"/>
    <dbReference type="KEGG" id="lth:KLTH0C04378g"/>
    <dbReference type="eggNOG" id="KOG1009">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010127_0_0_1"/>
    <dbReference type="InParanoid" id="C5DDW7"/>
    <dbReference type="OMA" id="QIYWHES"/>
    <dbReference type="OrthoDB" id="71227at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033186">
        <property type="term" value="C:CAF-1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006335">
        <property type="term" value="P:DNA replication-dependent chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055410">
        <property type="entry name" value="Beta-prop_CAF1B_HIR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045145">
        <property type="entry name" value="PTHR15271"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15271">
        <property type="entry name" value="CHROMATIN ASSEMBLY FACTOR 1 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15271:SF4">
        <property type="entry name" value="CHROMATIN ASSEMBLY FACTOR 1 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24105">
        <property type="entry name" value="Beta-prop_CAF1B_HIR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 9" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="CAF1B/HIR1 beta-propeller" evidence="11">
        <location>
            <begin position="6"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="65"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="130"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="447"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="463"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="491"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF24105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR21978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="501" mass="55983" checksum="1873CB71F2A27305" modified="2009-07-28" version="1">MEASNLQIYWHESQPIYSLCFQSTGPPKARRLVTAGGDNKIRVWQLNFEERNAFRVDSIDFLSSLTQHEQAVNVVRFNSTDDILATAGDDGQLLLWKKNDSMAKEFGVDEEEFADFKESWHVWKRLRPATTGGASEIYDLCWSPDDNYIVTGSMDNSVRVFDVNEGSCVASAVDHNHYVQGVAWDPQNEFILSQSADRSVHLHKLVRDAGGAISGLKFSSKITKGDLPCRKAPNSTELELDNVKSAFLFHNETLPSFFRRLAISPCGNLVCVPAGIFKNCDSQSESGNGNDELANAVYLFTRAYLEKHNNRPSLVIPFLKKPALVISFNPHLYQLSKQDHPCVKLPYKLLFAVATSDEVLIYDTETAKPVCIVGNLHYTPITDLSWSKDGAMLMISSTDGFCSYISFKNEMLGNLYDNEVQKGIQTNEAISTAPIKKPSIVNILPIKRKSSPRDKNPSEQITSAQVESKVQVQQVSNISKPSDSHDETKPTKRRVKPTLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-04-02" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDY1</accession>
    <name>C5DDY1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0C04730p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0C04730g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552430.1">
        <property type="nucleotide sequence ID" value="XM_002552384.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDY1"/>
    <dbReference type="STRING" id="559295.C5DDY1"/>
    <dbReference type="GeneID" id="8291297"/>
    <dbReference type="KEGG" id="lth:KLTH0C04730g"/>
    <dbReference type="eggNOG" id="ENOG502SDHJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1230131_0_0_1"/>
    <dbReference type="InParanoid" id="C5DDY1"/>
    <dbReference type="OMA" id="PLEWDEN"/>
    <dbReference type="OrthoDB" id="4036638at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020401">
        <property type="entry name" value="mRNA_transport_factor_GFD1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17331">
        <property type="entry name" value="GFD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="30"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="136"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="224" mass="24756" checksum="BC38DA2C4D2DE824" modified="2009-07-28" version="1">MSLNSSIWASPSTDSEDRSALDSKTNGLGSSMWASASVSSTQRKSRRKSSSCNRKKEPSRTNKQEKPSKPSTGNALAARLGMLDLNKPLDSPQKQEGPKKNRRASPPQEKKSSNSLAARLGMVEDSESSGDSDSEDVTRPSTSDKKSIFDRIKPKRETKNDSSLERGSGAGKQAAKHGSKMELLKQKVEEQKRIKETNQHKSQQTELLKDFLNDDASFNWEDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DE26</accession>
    <name>C5DE26_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Condensin complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0C05764g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the CND2 (condensin subunit 2) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552475.1">
        <property type="nucleotide sequence ID" value="XM_002552429.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DE26"/>
    <dbReference type="FunCoup" id="C5DE26">
        <property type="interactions" value="917"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DE26"/>
    <dbReference type="GeneID" id="8291343"/>
    <dbReference type="KEGG" id="lth:KLTH0C05764g"/>
    <dbReference type="eggNOG" id="KOG2328">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010510_0_1_1"/>
    <dbReference type="InParanoid" id="C5DE26"/>
    <dbReference type="OMA" id="GREHWKV"/>
    <dbReference type="OrthoDB" id="362021at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000796">
        <property type="term" value="C:condensin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022816">
        <property type="entry name" value="Condensin_barren_su2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13108">
        <property type="entry name" value="CONDENSIN COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13108:SF9">
        <property type="entry name" value="CONDENSIN COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05786">
        <property type="entry name" value="Cnd2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017126">
        <property type="entry name" value="Condensin_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7 11" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 11" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="8 11" id="KW-0498">Mitosis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="240"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="374"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="410"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="470"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="655"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="291"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="481"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR017126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR22037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="792" mass="88544" checksum="0B0D6D32CD4340B4" modified="2009-07-28" version="1">MALNVLYQAVLVRGFGNTPSSIRTIMADTIVADDDDRGLFTNKSTMMANFEQWIKMATDNKINSRNSWNFALIDYFHDLNVLKDAEDNINFQKASATLDGCVKIYSSRVDSVANETGILLSGLAQRKNQQQQGDDESGAEGAKTIENVVEGGVTIDPSTGLPISNEVDTTSRKRNYNRVLETTLVDFETLRMRELDQELSIDPLFKKALADFDEGGAKSLLLNTLHIDSSARVVFDATASHSQRNSGKPDLVEQHETSNISGEMDEDSVVSDSSHEEAEGSVLGGSGPVEQASNSHDSNMSTNHGSDHETIDVEDEILALGMDFIDFKLINEAEICPSMKSLKDVVEDINKAKSFIDDVNSKFDNFLTEKDLQSAIPDPQEDEGASYDLHDDGSLGADAGLYEDAGAMEDDQIDDPVGDHSGATDGDRSTVDPITAVMDRDVMAYFDETMKKSWRGREHWKVRNFKDRFLPKTNDNATNDTHGDAQGSEKGRDTNKPKKPPKQKFTLDFFNFNNIEEQIFDNKRKANIEMPQKLRTNPTRYLLPDDYQFSTQKVTRLFIKPQQHMSFFSHRKSKADNQRPGGILKFDEIDAHEDSQVAELADQNFWAQNYQMREQEKNVMEEEIDRPDMTAGLQNPFDDGEVDFNQAFGSEALDEFDSGSIDPPPGGSAGLTQPFGMPEGKVTYSRVSKKVDVKRLKDNIWKSISTTLQAQQKENSEQQVNEEDAAGRSTLKFTDIAENISTMYNGERKKDLSTSFCFICLLHLANEHGFEINPTEDFTDLAVEFQAEKDNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEF9</accession>
    <name>C5DEF9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA-5-taurinomethyluridine 2-sulfurtransferase</fullName>
            <ecNumber evidence="3">2.8.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0C08844g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the 2-thiolation of uridine at the wobble position (U34) of mitochondrial tRNA(Lys), tRNA(Glu) and tRNA(Gln). Required for the formation of 5-taurinomethyl-2-thiouridine (tm5s2U) of mitochondrial tRNA(Lys), tRNA(Glu), and tRNA(Gln) at the wobble position. ATP is required to activate the C2 atom of the wobble base.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>5-taurinomethyluridine(34) in tRNA + S-sulfanyl-L-cysteinyl-[protein] + AH2 + ATP = 5-taurinomethyl-2-thiouridine(34) in tRNA + L-cysteinyl-[protein] + A + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:47040"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11726"/>
            <dbReference type="Rhea" id="RHEA-COMP:11732"/>
            <dbReference type="Rhea" id="RHEA-COMP:11733"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61963"/>
            <dbReference type="ChEBI" id="CHEBI:87171"/>
            <dbReference type="ChEBI" id="CHEBI:87172"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.8.1.14"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MnmA/TRMU family.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.14" evidence="3"/>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552608.1">
        <property type="nucleotide sequence ID" value="XM_002552562.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEF9"/>
    <dbReference type="FunCoup" id="C5DEF9">
        <property type="interactions" value="399"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEF9"/>
    <dbReference type="GeneID" id="8291482"/>
    <dbReference type="KEGG" id="lth:KLTH0C08844g"/>
    <dbReference type="eggNOG" id="KOG2805">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035188_1_2_1"/>
    <dbReference type="InParanoid" id="C5DEF9"/>
    <dbReference type="OMA" id="PFYVWDL"/>
    <dbReference type="OrthoDB" id="3685at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016783">
        <property type="term" value="F:sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002143">
        <property type="term" value="P:tRNA wobble position uridine thiolation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01998">
        <property type="entry name" value="MnmA_TRMU-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.280:FF:000001">
        <property type="entry name" value="tRNA-specific 2-thiouridylase MnmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000115">
        <property type="entry name" value="tRNA-specific 2-thiouridylase MnmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.280">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004506">
        <property type="entry name" value="MnmA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046885">
        <property type="entry name" value="MnmA-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046884">
        <property type="entry name" value="MnmA-like_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023382">
        <property type="entry name" value="MnmA-like_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001138">
        <property type="entry name" value="PRK00143.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00420">
        <property type="entry name" value="trmU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11933:SF5">
        <property type="entry name" value="MITOCHONDRIAL TRNA-SPECIFIC 2-THIOURIDYLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11933">
        <property type="entry name" value="TRNA 5-METHYLAMINOMETHYL-2-THIOURIDYLATE -METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03054">
        <property type="entry name" value="tRNA_Me_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20258">
        <property type="entry name" value="tRNA_Me_trans_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20259">
        <property type="entry name" value="tRNA_Me_trans_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="4" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="tRNA-specific 2-thiouridylase MnmA-like central" evidence="13">
        <location>
            <begin position="246"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA-specific 2-thiouridylase MnmA-like C-terminal" evidence="12">
        <location>
            <begin position="355"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF20258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF20259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR22170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="426" mass="47801" checksum="C323682AE38F01DC" modified="2009-07-28" version="1">MACLKPFEQLAKFAASQCRQVPGYQPMKLPSKFDNIVIAMSSGVDSSVAAAMFSGFPNACGIYMQNWTETRSEREPCHERDWKDVQKVGAHLNLPVEKVNFEQDYWLDVFEPMLRDYEQGVTPNPDIGCNRFIKFGKLRAHLDASLGTGNYWLVTGHYSRVLQTESKALGLNSWHLLRAFYAPKDQSYYLSQVPSAALPALCLPMGVLTKPEVREMAHDLRLPTAHKPDSQGICFVANSQHGKISRFLEQYIPSHAGEIITLDPITGERTEWGRHTGLWNYTIGQKVGISMPQGDPRYQGAWFVADKRVDTNEIVICKGRDNPSLYKNRVVLKDFSPLGNLNDVQEAVKNNLAGGAIHIQYRSLQEPAKVSRCCWETTEAGESRLVMTLEQKQRAMAPGQYCTIYHKERILGSGTILASDAVEANK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-04-02" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>C5DET2</accession>
    <name>C5DET2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0C11946p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0C11946g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552731.1">
        <property type="nucleotide sequence ID" value="XM_002552685.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DET2"/>
    <dbReference type="STRING" id="559295.C5DET2"/>
    <dbReference type="GeneID" id="8291622"/>
    <dbReference type="KEGG" id="lth:KLTH0C11946g"/>
    <dbReference type="eggNOG" id="ENOG502RQMA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_615495_0_0_1"/>
    <dbReference type="InParanoid" id="C5DET2"/>
    <dbReference type="OrthoDB" id="4036194at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950352" evidence="2">
        <location>
            <begin position="19"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="292"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="336"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="301"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="336"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="407"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="435"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR22293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="445" mass="45262" checksum="B615CF8E8938FF09" modified="2009-07-28" version="1">MVRKAHLPLLASIASACATCPSISDVNVLSADDGSSSFWGTGLGAYIELTLSATEKGECWFSAPSNVFGNFPACPFSWDYGTVNSAGGNNYSIQFNQIPHGGVAKLGFLTSLAEGYKSSITSGKTDTYSFVTSSGEHKSSVSFNDFPSDQVYCGSCGTQQEGNSLSFDVSVPAAIIESAFTINIEKQQGYKFQTGGIKLYYTQSSNSFDEASNEQLVPSSSITDSSTEDKISIAFNGNLPSGCSGLRLSYDVAVLEEMTYYSSVCNVEFSSFSQQLTAYVYTKNHMGSIETPAVSKGPGCGSQTSSSSGYSSSTVTTLSPSTGFTSSSTSAAVTSATSSSGCDDGCDDGSGSSSSSSSPAAPTSSSGCDSGCDDNTATSSATPSTSTSSSSSSGTSCDSGCDENSGSGSGSGSGHGSGHYAGHVGRHNWGHNSGHKGGQGSGCKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEZ8</accession>
    <name>C5DEZ8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0D11088p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0D11088g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553191.1">
        <property type="nucleotide sequence ID" value="XM_002553145.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEZ8"/>
    <dbReference type="FunCoup" id="C5DEZ8">
        <property type="interactions" value="46"/>
    </dbReference>
    <dbReference type="GeneID" id="8295431"/>
    <dbReference type="KEGG" id="lth:KLTH0D11088g"/>
    <dbReference type="eggNOG" id="ENOG502RYS8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046455_1_0_1"/>
    <dbReference type="InParanoid" id="C5DEZ8"/>
    <dbReference type="OMA" id="HINVEWA"/>
    <dbReference type="OrthoDB" id="4073963at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR22753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="422" mass="47242" checksum="E7C26D79882BD6D2" modified="2009-07-28" version="1">MAPRESLPDCWTTKIQGADIDNGSMRVRPSRLVSVSSMLGSGVGASSRSSSRWKQEAHDLFGLKRLARAYFRGEEHYTKEDVPDYGVMRFPPKGTRLRKNQTSSATLIKPNCHAPQTTDGSMKPLLPQKKHCYSVSMGSLIEGRDVNVKPLKTPVAVESGENVSQFGDIKGLKKSTLNSSALLNKHHVNAAFLQNSHQDSENGEERVVEILELPQDVGIQSLLSQVYGGPLEKVNVSKDQRGAPARGARLHFLFNEDAKSFISYGLSNNFRINGHIVKPQWAPKSTCSLSNMNFDEVKDMVSANACAGRQEGTRARRCIVVKKTGCTKSPRSRQLHSNRQNLCSFDYKDLRKDFEQFGEISEISPMISRKLCVSVSFYDVRSAINALESFGEPDSFMHKKYNRDWTMAYGRDTTDRACYHMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>C5DF14</accession>
    <name>C5DF14_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">leucine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Leucyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0D11440g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>tRNA(Leu) + L-leucine + ATP = L-leucyl-tRNA(Leu) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11688"/>
            <dbReference type="Rhea" id="RHEA-COMP:9613"/>
            <dbReference type="Rhea" id="RHEA-COMP:9622"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78494"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.4" evidence="2"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553207.1">
        <property type="nucleotide sequence ID" value="XM_002553161.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DF14"/>
    <dbReference type="FunCoup" id="C5DF14">
        <property type="interactions" value="1256"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DF14"/>
    <dbReference type="GeneID" id="8295447"/>
    <dbReference type="KEGG" id="lth:KLTH0D11440g"/>
    <dbReference type="eggNOG" id="KOG0437">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004174_1_1_1"/>
    <dbReference type="InParanoid" id="C5DF14"/>
    <dbReference type="OMA" id="KFIEWQF"/>
    <dbReference type="OrthoDB" id="10249672at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004823">
        <property type="term" value="F:leucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006429">
        <property type="term" value="P:leucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07959">
        <property type="entry name" value="Anticodon_Ia_Leu_AEc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.740.10:FF:000001">
        <property type="entry name" value="Leucine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="entry name" value="Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004493">
        <property type="entry name" value="Leu-tRNA-synth_Ia_arc/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055416">
        <property type="entry name" value="RBD_LARS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00395">
        <property type="entry name" value="leuS_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008957">
        <property type="entry name" value="PRK12300.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45794:SF1">
        <property type="entry name" value="LEUCINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45794">
        <property type="entry name" value="LEUCYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24810">
        <property type="entry name" value="RBD_LARS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="10">
        <location>
            <begin position="56"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="10">
        <location>
            <begin position="210"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding" evidence="11">
        <location>
            <begin position="817"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine--tRNA ligase RagD-binding" evidence="12">
        <location>
            <begin position="961"/>
            <end position="1015"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF24810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR22769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1098" mass="125140" checksum="F68DE150C5956448" modified="2009-07-28" version="1">MSSDTGSKGLVLENTARRDALIAIEKKYQKLWAEEHVFEIDAPSIEEESVSIDSEELQRKYPKFMSSMAYPYMNGVMHAGHCFTLSKVEFSVGFERMNGKRALFPLGFHCTGMPILACADKLKREAELFGSDFSKAPAEEEEDEAAAAKEEVKTESEDVTKFKAKKSKAAAKKGRGKFQFEIMLQLGIPHEEVVKFADPQFWLTYFPPLCQSDCSAFGSRIDWRRSFITTDMNPYYDAFIRWQMNKLKELGKIKFGERYTIYSEKDGQACMDHDRQSGEGVTPQEYVGIKIETLEFAEAAKKVVESSDALDKSKRFYFVAATLRPETMYGQTCCFVSPKINYGIFDAGDAYYITTERAFKNMSYQKLTPKRGYYKPLVTISGKDFIGSKIRAPLSAYDELRILPMETVIATKGTGVVTCVPSNSPDDYMTTRDLQNKSQFYGIDPEWVKHEPVPIIRSERYGDLTAQKICEELKIRSPKDTVPLAEAKKIAYKEDFYNGTMIYGKFKGEKVEVAKGKLKAELIANNEAFVYNEPESLVVSRSGDDCIVSLEDQWYVDYGEQTWKEQAIECLNQMNTFAPEVKNAFEGCLEWLKNWAVCRTYGLGTKLPWDEKYLVESLSDSTIYQAFYTIAHLLFKDYYGNEIGPLGIEANQMTPAVFDYIFQHTDNVETDIPIESLQKLRREFEYFYPLDVSISGKDLITNHLTFFIYTHVALFPKKFWPRGIRANGHLMLNNAKMSKSTGNFMTLKQIVEKFGADASRIALADAGDTIEDANLDETNANAAILRLYNLKEWADEVVKNLDGLRSGPITEFFDVSFENELNALVEETYKQYELTNYKSALKTGLFDLQTARDYYRESCEVMHRDLVLRYIEFQVLVLAPIAPHFAEYIWREVLGNTKSVQITRFPRATKPVDAGALSALEYLRNLQRSIRESEGQALKKKKGKGSDVDITKPAKMTLLISESFPEWQSEYIELVRKLFESQELDDNKKIKQMVNPKDMKRAMPFISMLKQRLVAEPVETVFNRELPFDEVATIKATVENVKKCCQVIKCQEFQFISFPYGAKVGKDIFTGEQVDIPNAAKVVENAIPGNPGIIIANI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>C5DF36</accession>
    <name>C5DF36_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cell division control protein 31</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0D11924g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553229.1">
        <property type="nucleotide sequence ID" value="XM_002553183.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DF36"/>
    <dbReference type="FunCoup" id="C5DF36">
        <property type="interactions" value="1000"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DF36"/>
    <dbReference type="GeneID" id="8295469"/>
    <dbReference type="KEGG" id="lth:KLTH0D11924g"/>
    <dbReference type="eggNOG" id="KOG0028">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061288_18_1_1"/>
    <dbReference type="InParanoid" id="C5DF36"/>
    <dbReference type="OMA" id="EFFMIMK"/>
    <dbReference type="OrthoDB" id="343296at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005825">
        <property type="term" value="C:half bridge of spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030474">
        <property type="term" value="P:spindle pole body duplication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000352">
        <property type="entry name" value="Cell division control protein 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000077">
        <property type="entry name" value="Centrin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF48">
        <property type="entry name" value="CENTRIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="35"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="108"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="144"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00070017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR22791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="176" mass="20570" checksum="5A431BD0E3F16E54" modified="2009-07-28" version="1">MSHRRTTPRGSLNGAGSYKPSNSENSVLQRELLEENKQEIFEAFSLFDMNNDGFLDYHEFKVAARALGFEMSKTELLELLEKYDRDGRRLIHYDDFFLVMGERILERDPLDEIRRAFKLFDDDNTGKISLKNLRRVAKELGENLTDDELRAMIDEFDLDNDGEISEREFIAICTDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFL7</accession>
    <name>C5DFL7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">ATP-dependent DNA helicase PIF1</fullName>
            <ecNumber evidence="16">5.6.2.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">DNA 5'-3' helicase PIF1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">DNA repair and recombination helicase PIF1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">PIF1</name>
        <name evidence="20" type="ordered locus">KLTH0D16148g</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of both mitochondrial and nuclear genome stability. Efficiently unwinds G-quadruplex (G4) DNA structures and forked RNA-DNA hybrids. Resolves G4 structures, preventing replication pausing and double-strand breaks (DSBs) at G4 motifs. Involved in the maintenance of telomeric DNA. Inhibits telomere elongation, de novo telomere formation and telomere addition to DSBs via catalytic inhibition of telomerase. Reduces the processivity of telomerase by displacing active telomerase from DNA ends. Releases telomerase by unwinding the short telomerase RNA/telomeric DNA hybrid that is the intermediate in the telomerase reaction. Involved in the maintenance of ribosomal (rDNA). Required for efficient fork arrest at the replicaion fork barrier within rDNA. Involved in the maintenance of mitochondrial (mtDNA). Required to maintain mtDNA under conditions that introduce dsDNA breaks in mtDNA, either preventing or repairing dsDNA breaks. May inhibit replication progression to allow time for repair. May have a general role in chromosomal replication by affecting Okazaki fragment maturation. May have a role in conjunction with DNA2 helicase/nuclease in 5'-flap extension during Okazaki fragment processing.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 16">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="16">Monomer. Interacts with telomerase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Matrix side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the helicase family. PIF1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.3" evidence="16"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553410.1">
        <property type="nucleotide sequence ID" value="XM_002553364.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFL7"/>
    <dbReference type="FunCoup" id="C5DFL7">
        <property type="interactions" value="870"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFL7"/>
    <dbReference type="GeneID" id="8295657"/>
    <dbReference type="KEGG" id="lth:KLTH0D16148g"/>
    <dbReference type="eggNOG" id="KOG0987">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001613_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFL7"/>
    <dbReference type="OMA" id="VQPEDWA"/>
    <dbReference type="OrthoDB" id="432234at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051880">
        <property type="term" value="F:G-quadruplex DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160225">
        <property type="term" value="F:G-quadruplex unwinding activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010521">
        <property type="term" value="F:telomerase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032211">
        <property type="term" value="P:negative regulation of telomere maintenance via telomerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18037">
        <property type="entry name" value="DEXSc_Pif1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18809">
        <property type="entry name" value="SF1_C_RecD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001226">
        <property type="entry name" value="ATP-dependent DNA helicase PIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03176">
        <property type="entry name" value="PIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010285">
        <property type="entry name" value="DNA_helicase_pif1-like_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049163">
        <property type="entry name" value="Pif1-like_2B_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051055">
        <property type="entry name" value="PIF1_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048293">
        <property type="entry name" value="PIF1_RRM3_pfh1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47642">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47642:SF5">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05970">
        <property type="entry name" value="PIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21530">
        <property type="entry name" value="Pif1_2B_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 16" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11 16" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="12 16" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9 16" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 16" id="KW-0347">Helicase</keyword>
    <keyword evidence="6 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13 16" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10 16" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14 16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA helicase Pif1-like DEAD-box helicase" evidence="18">
        <location>
            <begin position="292"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="domain" description="DNA helicase Pif1-like 2B" evidence="19">
        <location>
            <begin position="555"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="DNA-binding region" evidence="16">
        <location>
            <begin position="778"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="209"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="837"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="209"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="313"/>
            <end position="320"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF05970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF21530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CAR22972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="899" mass="101318" checksum="AF8A1D9DC5FA29B3" modified="2009-07-28" version="1">MKIASVALRLSNIHNRRLPLNFYRYHMKTFATSPIAQRGPIKRAKTWDSKELCQLLSDSDGWDDLIDSPVKQPVETLGQSRFEMSPCRIKEQDEDDDDDDILLLSENWVQKRGKTCDINSTHRVNSVPQTKDTTALTSSHSKQLDQFLRRASSKRDPTVELPKTSFFKLTEPKPVKDALYAPAVRTAQSGNTEQNLKSEAEEPFSSILNQPTAGSQLSTSPSFEDPDCPPLSLSMEEEEEGSVTNGRKRSSVRSRNHELVFLTQKAEREGSEELASRTNSTLTKKLVQGLVLSEEQESVIELAKERYNIFYTGSAGTGKSVLLRVLIKTLRRMYGPGSVAVTASTGLAACNIGGITVHSFAGFGLGNGDSKILLKKVRRSKKHVKRWASIKALVIDEVSMIDGDLLDKLDYVARSIRKDQSPFGGIQVILSGDFFQLPPVNKNQGIETKFAFHADAWKEAIDATIMLQKVFRQQGDSEFVEMLNEMRMGKISLETELEFKKLSRPLPDDDIIPAELYSTRNEVERANMSRLKNLPGECHTYHAIDGGNLRDQETKEKLLSNFLAPKELRLKVGAQVMMIKNIDDTLVNGSLGKIIDFIDKDTYMFYERVRSYPDIDIEELELMRQGKIPMGESSEEEEMNQRVIRKKTLKESFCGTDAVVTGERLGESIFDFLLANFDKLSASQRLNIERKKKLLEEVQASSSGRKLPLVRFLTPDGTSRCVLVQPEDWAVEDELQKPIVSRVQLPLMLAWALSIHKSQGQTLPKVKVDLRRIFEKGQAYVALSRAVSRDGLQVLNFNKAKVQAHGSVMEFYQTLTSAHEAKKRFSSTKGKQQALYFPQRAAQPAKRTNSNTKEERQDRITSMLMRKNKSSMADAKLKDTGLMSFSYQSDLQCDQNQNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFT1</accession>
    <name>C5DFT1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0D17688p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0D17688g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR23036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553474.1">
        <property type="nucleotide sequence ID" value="XM_002553428.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFT1"/>
    <dbReference type="FunCoup" id="C5DFT1">
        <property type="interactions" value="542"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFT1"/>
    <dbReference type="GeneID" id="8295725"/>
    <dbReference type="KEGG" id="lth:KLTH0D17688g"/>
    <dbReference type="eggNOG" id="KOG4673">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018551_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFT1"/>
    <dbReference type="OMA" id="NWESIEF"/>
    <dbReference type="OrthoDB" id="74178at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052602">
        <property type="entry name" value="Growth_transcription_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022092">
        <property type="entry name" value="TMF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022091">
        <property type="entry name" value="TMF_TATA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46515:SF1">
        <property type="entry name" value="TATA ELEMENT MODULATORY FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46515">
        <property type="entry name" value="TATA ELEMENT MODULATORY FACTOR TMF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12329">
        <property type="entry name" value="TMF_DNA_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12325">
        <property type="entry name" value="TMF_TATA_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TATA element modulatory factor 1 TATA binding" evidence="6">
        <location>
            <begin position="561"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="54"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="224"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="463"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="545"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="373"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="571"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="549"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR23036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="680" mass="77261" checksum="922AA065D3C64D4A" modified="2009-07-28" version="1">MIMEYQCLVDQGCRHHGISSYEPSCHQSLALTLALMSNSNKKLSIEERLSLAARAKSRRKGKKPDSPTPSRATSDLNIEAKGSESPDLVQPAGREEEIHVELAKDGPSSACELDAILPSDYRDLPAETVVDILKPYLRKHVESLAPRGQPSGSSLMKLIREKDNIIEELREEGENLSKLELRNNSTIKELRDKLKATEKSLDSKKKLLDAKSAANALLENRLEEMQSQRKELERQLDSTTSERDRATKDTEELKTEVLSKLKKSLEDAESQSAQSQLQVRRLRDQNEEISIKASLKYQTLDETSRSEITRLETKLEEMRIKLYQFSSTQPKGGAVENSASGSKLLSKYNALENQLKASRGNWSSIESSFRQRTYELEAKLESTQRALDAAEEALGAAKKNQTSLKSAISFYKAEKSSLEKEVSRLTDITQSLEIDLRNVREDLKLWQEKYRIQKLELEEHLVKPKKPLDSPREDNQKARPERPSNGEQKIERISDWEIQDIENLQESFENENETSGLGSQYSLGEFSQNEEIEFSSSLRKSSLASLDLTPSQQQKTVVKPSQNSQMNAQMVSRLGSQIRRLETELASLKDSNSRLLKEKQNINDTIVKLMEENSNANKTKSQLKESLAHSDALEKKLNATLQLLGERSERVEELENDVDDLKELMQMQVQQMVELQERVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFT3</accession>
    <name>C5DFT3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0D17732p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0D17732g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR23038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553476.1">
        <property type="nucleotide sequence ID" value="XM_002553430.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFT3"/>
    <dbReference type="FunCoup" id="C5DFT3">
        <property type="interactions" value="396"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFT3"/>
    <dbReference type="GeneID" id="8295727"/>
    <dbReference type="KEGG" id="lth:KLTH0D17732g"/>
    <dbReference type="eggNOG" id="ENOG502QSYR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037435_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFT3"/>
    <dbReference type="OMA" id="RFAFQAN"/>
    <dbReference type="OrthoDB" id="30195at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="342"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="345"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="551" mass="61950" checksum="CBA398478F2E1DB6" modified="2009-07-28" version="1">MTANSELISSFSPDAQNFAFKSNSLQRKYVDLYPLSNINDYKVTSSLVNHIDYDSGDLKLDDLKYIAWCSSSARGGATSSKRRNAGVESSNGDVPSSVPESYFVNVFKGGKIVIFSPTGKDIINIIQNRHEILGVGSTGPIIWILDEEKTVKEFDYRSSKPLKTFHLNDGKEEDIAHFELLDVQGKPTIAIFTDSLLYVIDPSKRRPSTTLKINVDRGRRCIPGPTGRIIVAGSEEIQVIELKTGSVVCRRPFLASEIKFSNGKVYAQSMAGELHILRAEDLEATCLIKCQLSKILHFTFVDSSVIIAWLNVNEPRFEHISLKQTTEEDEIIFQLEDSSNVHNARPKEETGGAALDNKESRSKVSKDKQRDILASLILLLDEREQSDKILENIRCEVWSENLIKNAVVSDLSEQHVRLVFDVISKEIASNPWSASRNYALWFKWLLTFYHVILLSGQDNSSNKKLKQVKSSLRTSSDVYSQLLAMKGKLEMLSEQAKLREEFSRTSLEDEITQSQGGENFEEKELVYANGEGDEYVDALEYAEKPPQINGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>C5DG03</accession>
    <name>C5DG03_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0D01364p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0D01364g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552783.1">
        <property type="nucleotide sequence ID" value="XM_002552737.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DG03"/>
    <dbReference type="FunCoup" id="C5DG03">
        <property type="interactions" value="26"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DG03"/>
    <dbReference type="GeneID" id="8295003"/>
    <dbReference type="KEGG" id="lth:KLTH0D01364g"/>
    <dbReference type="eggNOG" id="ENOG502QZN7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061844_0_0_1"/>
    <dbReference type="InParanoid" id="C5DG03"/>
    <dbReference type="OMA" id="DFIWYSI"/>
    <dbReference type="OrthoDB" id="2567806at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005675">
        <property type="term" value="C:transcription factor TFIIH holo complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031349">
        <property type="entry name" value="Tfb6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37781:SF1">
        <property type="entry name" value="ADR380WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37781">
        <property type="entry name" value="TFIIH COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17110">
        <property type="entry name" value="TFB6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="293" mass="33875" checksum="CE2716EA86C5AE3B" modified="2009-07-28" version="1">MIKTPDTPEHTGANEELNVDEIHEIDENDVEDLDLGYGNEDEQQNDRSSLKREASTDDDIVFDQKDEFVPQVNISSPFSSTANITRLRDSHSSAQGRLSMSQQSKFVSYCDDRLMEIQRKFVQSRGLAEENGYRELSPLLQDLKSLLDFIWYSIEGIPNTELLLKQNLDDMLARQFANTRSTNFGQTYYLIRVADDFLDYLTKFDLTHLKVEEQQKTVAKVFKFLMILDKVFAKCLEGLIPGNLKMNGTEMVRISGIAERTRMVLPQYLEQQKIHGYHYEVSKVYEETLERCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>C5DG69</accession>
    <name>C5DG69_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0D02838p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0D02838g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552849.1">
        <property type="nucleotide sequence ID" value="XM_002552803.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DG69"/>
    <dbReference type="FunCoup" id="C5DG69">
        <property type="interactions" value="146"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DG69"/>
    <dbReference type="GeneID" id="8295069"/>
    <dbReference type="KEGG" id="lth:KLTH0D02838g"/>
    <dbReference type="eggNOG" id="ENOG502QWM7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008595_1_0_1"/>
    <dbReference type="InParanoid" id="C5DG69"/>
    <dbReference type="OMA" id="MQKMCDC"/>
    <dbReference type="OrthoDB" id="4097064at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000812">
        <property type="term" value="C:Swr1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140849">
        <property type="term" value="F:ATP-dependent H2AZ histone chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037651">
        <property type="entry name" value="Swc3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058986">
        <property type="entry name" value="Swc3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057558">
        <property type="entry name" value="Swc3_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28108">
        <property type="entry name" value="SWR1-COMPLEX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28108:SF1">
        <property type="entry name" value="SWR1-COMPLEX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24707">
        <property type="entry name" value="Swc3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26242">
        <property type="entry name" value="Swc3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SWR1-complex protein 3" evidence="2">
        <location>
            <begin position="41"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="Swc3 C-terminal" evidence="3">
        <location>
            <begin position="392"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="165"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="300"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="457"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="165"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="236"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="310"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="346"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="463"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF26242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR22411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="584" mass="66693" checksum="E661508A66E223B9" modified="2009-07-28" version="1">MSRSSRLRTRTVGAERSRSSKRGKSEEELDTYGLVEENGGRPFQLIEGLPCSIELPQYASTLTHPLSVKDSAVLYSSMLSSRRTWISSEMFDMLWSKQYLSPSEKERLEQEGIDPESVDASAAREKMHKLCDCAMAGGPHAFSVRLFILKNDEIEKKWNDAIEQKRRNKADRKNRELEEKKKKQEERKQQQWIKKQEKEHGTSTAADSSRNSGSTDGAKKPVKRRSRKEDTTQQRSRSHTPRQRKSVPQSTEDQKMITNLNLMAQKNPELNSLMIVVAGGRASTDQVEEFKKYIEKARKMPVPPGWKPTANASSSTPQKLKSGGSDSSLDRADSVAAPARAAKSLGASQVKSEETAQLSSETNSAGGTAEPRKKRKYTKRKNVDTPVEPEDKSMQLTTFQQKYMHNADIVFEYVENPNKRFSLPKDAILEPLEDGESYLMSWILIHNRKELEKYKEKRKKTLDKKAKPEKTAEVSTEDSANKEEEDDYDIFEDPKAPDPLFTPMTVKFSNIHKKFQPIMTNSVNPAEKVRKRMSNIIERGTRLSGYNLWFQLDAYDDSGFAESLRCDLKDYESGFRSKRHRKQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGF1</accession>
    <name>C5DGF1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">KLTH0D04818g</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552931.1">
        <property type="nucleotide sequence ID" value="XM_002552885.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGF1"/>
    <dbReference type="FunCoup" id="C5DGF1">
        <property type="interactions" value="221"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGF1"/>
    <dbReference type="GeneID" id="8295158"/>
    <dbReference type="KEGG" id="lth:KLTH0D04818g"/>
    <dbReference type="eggNOG" id="KOG0598">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_120_2_1"/>
    <dbReference type="InParanoid" id="C5DGF1"/>
    <dbReference type="OMA" id="SWKRLLM"/>
    <dbReference type="OrthoDB" id="63267at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070941">
        <property type="term" value="P:eisosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060237">
        <property type="term" value="P:regulation of fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006665">
        <property type="term" value="P:sphingolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11651">
        <property type="entry name" value="YPK1_N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000008">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000048">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="358"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="17">
        <location>
            <begin position="614"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="13"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="14"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="81"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="391"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAR22493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="691" mass="77792" checksum="99D28067533A8C02" modified="2009-07-28" version="1">MYSWKLASKFKFGRSKEEKEKEKEREKNKDKEKHGLFHHKSHRSESPYMEPPRGSVDRKETIVPSSSSIAPVRLSSDVNKSSATNSSASTGRTSMSTSGDQDTVEDKNAASSFVGDKSSGKDSLIEEPRFSGIMTIKVYGGEGFMLPVSITSNVQILKKLLQSGVINQASDDANVDELISSLSQLQVADPGDDENIIDGETAARYIPATITLPTSTTLNPLLYFTLEFDNTVATIEPESGTISNLTFNKISTFDVTRKLNFLKMDVFARIPSILLPSKAWQQMNSSDPYIKDILDRINSNQDIHVGSCNLPLTLDFDSASTFRLYNHQWISLEQGNGKLNISVDYKPATNRALSIDDFDLLKVIGKGSFGKVMQVRKKDTNKVYALKAIRKSYIVSKSEVTHTLAERTVLARVDNPFIVPLKFSFQSPDKLYIVLAFINGGELFYHLQREGRFSLSRARFYTAELLCALETLHNLDVIYRDLKPENILLDHQGHIALCDFGLCKLNMKGQEKTNTFCGTPEYLAPELLLGQGYSKVVDWWTLGVLLYEMMTGLPPYYDEDVPKMYKKILQEPLRFPDGFDKDAKDLLIGLLSRDPKRRLGFNGAEEIKSHPFFSQLNWKRLWMKGYIPPYKPPVMNSLDTSNFDQEFTKELPVDSVVNDFLSESVQQQFGGWTYVGSEQLGSSMAPTKSIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGP0</accession>
    <name>C5DGP0_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Golgi apparatus membrane protein TVP18</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Golgi apparatus membrane protein tvp18</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0D06864g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Golgi membrane protein involved in vesicular trafficking.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TVP18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553020.1">
        <property type="nucleotide sequence ID" value="XM_002552974.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGP0"/>
    <dbReference type="FunCoup" id="C5DGP0">
        <property type="interactions" value="70"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGP0"/>
    <dbReference type="GeneID" id="8295251"/>
    <dbReference type="KEGG" id="lth:KLTH0D06864g"/>
    <dbReference type="eggNOG" id="ENOG502S3AC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118698_1_0_1"/>
    <dbReference type="InParanoid" id="C5DGP0"/>
    <dbReference type="OMA" id="IYAQWLG"/>
    <dbReference type="OrthoDB" id="5591789at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019365">
        <property type="entry name" value="TVP18/Ca-channel_flower"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13314">
        <property type="entry name" value="CALCIUM CHANNEL FLOWER HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13314:SF2">
        <property type="entry name" value="CALCIUM CHANNEL FLOWER HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10233">
        <property type="entry name" value="Cg6151-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01077">
        <property type="entry name" value="Cg6151-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="29"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="53"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="99"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR22582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="167" mass="18418" checksum="3F57EF9C79590555" modified="2009-07-28" version="1">MALNIKQFINVAGLVRDLKSFNFSVYGQWFGYINIFLCVALGIANLFHVSAVIVFGIIAIVQGLVILFVEVPFLLRICPLSDNFIAFIKRFETNGKRTLFYVGMAAVQWCSLAVMATSLIAVAIGLTISAASYGVAFFKHQQFQGTSVIKSPADDDFPHEAVVREML</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGS6</accession>
    <name>C5DGS6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0D07898p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0D07898g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553056.1">
        <property type="nucleotide sequence ID" value="XM_002553010.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGS6"/>
    <dbReference type="FunCoup" id="C5DGS6">
        <property type="interactions" value="121"/>
    </dbReference>
    <dbReference type="GeneID" id="8295289"/>
    <dbReference type="KEGG" id="lth:KLTH0D07898g"/>
    <dbReference type="eggNOG" id="ENOG502RBGV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012010_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGS6"/>
    <dbReference type="OMA" id="GWNMSGG"/>
    <dbReference type="OrthoDB" id="4064873at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051711">
        <property type="entry name" value="Stress_Response_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47540">
        <property type="entry name" value="THIAMINE REPRESSIBLE GENES REGULATORY PROTEIN THI5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47540:SF2">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="8">
        <location>
            <begin position="31"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR22618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="672" mass="75210" checksum="1CCAC711DB06DF65" modified="2009-07-28" version="1">MCLDHHQQDDNARVATLQQDTGRPRIRVRKACGTCKRRKVKCNGQQPCAGCAKHGSVCHYKVEPVTRPASAALKAGAPGPAGTLPALSQVPAPSDFATGPAPASPWQRFSPGKYRFHRRHQNLVPYYLGQALISSLPPALVQKYALRAPRLQFYGWNMSGGHYLKQGSTFGAPQGTVWRWDLAVELQRQILEKCASFFFQHVNRFVSIVHEQAFWQQFRGGFLDGSDGKNGSSDLFEAILNLIAAIALRFSYSGAGASVCEALTAAEVTWLDLHLTRHGRHLEETLFERAYAVTTRLSFEWESFELIQAWLLAAVYLRTCHRQVSCWQALSRAVQMCNGMSLYLNRFPEKHTAYDECRARNCYWACFVLDRLISFQMGRFPELAGPGPDMGSPDTAVDTWFSAESVALYKLAAIVTTCQRRYGQELTAAETQELGARLDEWHGDCEEVLADPGLCARQVLLTYLDVRIALEIRGLFQLLDVGSDTVRHALPLDAVTLVDLASRVLDQFEAIVESGLFFRPWWLNLSLLFTCSVICLTLVQGGLQLARAQALLSRSFHIWHFIEGARPPNPPGMARECLWCLKMLNHMFCQRLTTSAEQLESIIGVDPGDDSVNQNKFRQFGKVDMNRANDDGAALLQSGSDTISPADDSNNLVAHLQWFDQWLDVDNLQTMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGY7</accession>
    <name>C5DGY7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ATP-dependent RNA helicase DBP2</fullName>
            <ecNumber evidence="3">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">ATP-dependent RNA helicase dbp2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">KLTH0D09328g</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="3"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553117.1">
        <property type="nucleotide sequence ID" value="XM_002553071.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGY7"/>
    <dbReference type="FunCoup" id="C5DGY7">
        <property type="interactions" value="1315"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGY7"/>
    <dbReference type="GeneID" id="8295354"/>
    <dbReference type="KEGG" id="lth:KLTH0D09328g"/>
    <dbReference type="eggNOG" id="KOG0331">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_16_9_1"/>
    <dbReference type="InParanoid" id="C5DGY7"/>
    <dbReference type="OMA" id="STMPKFE"/>
    <dbReference type="OrthoDB" id="196131at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010467">
        <property type="term" value="P:gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17966">
        <property type="entry name" value="DEADc_DDX5_DDX17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000008">
        <property type="entry name" value="ATP-dependent RNA helicase RhlB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000079">
        <property type="entry name" value="probable ATP-dependent RNA helicase DDX17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 13" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0866">Nonsense-mediated mRNA decay</keyword>
    <keyword evidence="4 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="17">
        <location>
            <begin position="111"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="15">
        <location>
            <begin position="142"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="16">
        <location>
            <begin position="345"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="500"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="12">
        <location>
            <begin position="111"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="20"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="503"/>
            <end position="536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAR22679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="551" mass="61006" checksum="49F9BBED098145FB" modified="2009-07-28" version="1">MTYGSRDNQFNRDNYGSRGGDFRGGRPSDRNSYGRDSYGGGRGGFSRPPPSQPMELVAPDWDQELPNLPKFEKNFYVEHESVRNRSDAEVEEFRKANEMSILGKDIPKPITTFDEAGFPEYVLSEVKAEGFEKPTGIQCQGWPMALSGRDMIGVAATGSGKTLSYCLPGIVHINAQPLLSPGDGPIVLVLAPTRELAVQIQTECSKFGKSSRIRNTCVYGGVPRGHQIRELTRGAEIVIATPGRLIDMLEIGKTNLKRVTYLVLDEADRMLDMGFEPQIRKIVDQIRPDRQTLMWSATWPKEVQQLARDYLHDPIQVNVGSLELAASHNIAQIVEVVSDMEKRDRLLKHLETASEDKDSKILIFASTKRTCDEITRYLRQDGWPALAIHGDKAQNERDWVLQEFRTGNSPIMVATDVAARGIDVKGINFVINYDMPGNIEDYVHRIGRTGRAGATGTAISFFTEANKGMGAQLISIMREANQQIPPELMKYDRRQYGGHPRYGRGGYGGGRGGRGGYGRGGRGGGRGYGGGRGGGFQKPRDSGWGNRNINY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>C5DH19</accession>
    <name>C5DH19_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">acetyl-CoA C-acyltransferase</fullName>
            <ecNumber evidence="10">2.3.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">KLTH0E00682g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>an acyl-CoA + acetyl-CoA = a 3-oxoacyl-CoA + CoA</text>
            <dbReference type="Rhea" id="RHEA:21564"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:90726"/>
            <dbReference type="EC" id="2.3.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; fatty acid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the thiolase-like superfamily. Thiolase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.16" evidence="10"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="RefSeq" id="XP_002553517.1">
        <property type="nucleotide sequence ID" value="XM_002553471.1"/>
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    <dbReference type="AlphaFoldDB" id="C5DH19"/>
    <dbReference type="FunCoup" id="C5DH19">
        <property type="interactions" value="363"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DH19"/>
    <dbReference type="GeneID" id="8291655"/>
    <dbReference type="KEGG" id="lth:KLTH0E00682g"/>
    <dbReference type="eggNOG" id="KOG1389">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031026_1_1_1"/>
    <dbReference type="InParanoid" id="C5DH19"/>
    <dbReference type="OMA" id="HAGEQSM"/>
    <dbReference type="OrthoDB" id="5404651at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003988">
        <property type="term" value="F:acetyl-CoA C-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010124">
        <property type="term" value="P:phenylacetate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00751">
        <property type="entry name" value="thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050215">
        <property type="entry name" value="Thiolase-like_sf_Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020615">
        <property type="entry name" value="Thiolase_acyl_enz_int_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020610">
        <property type="entry name" value="Thiolase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020613">
        <property type="entry name" value="Thiolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01930">
        <property type="entry name" value="AcCoA-C-Actrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43853">
        <property type="entry name" value="3-KETOACYL-COA THIOLASE, PEROXISOMAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43853:SF8">
        <property type="entry name" value="3-KETOACYL-COA THIOLASE, PEROXISOMAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02803">
        <property type="entry name" value="Thiolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00098">
        <property type="entry name" value="THIOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00737">
        <property type="entry name" value="THIOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00099">
        <property type="entry name" value="THIOLASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Thiolase N-terminal" evidence="14">
        <location>
            <begin position="28"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase C-terminal" evidence="15">
        <location>
            <begin position="287"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="12">
        <location>
            <position position="117"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="365"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000429-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAR23080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="407" mass="43063" checksum="10C6D2F5F8215B25" modified="2009-07-28" version="1">MSERLTQIKDHLISAVSPSVQDQRADDVVIIAAHRSPIARGFKGGFKDVNSDYLVHQFLQEFFKKVPSSLSENKQLIEEVACGNVLNPGAGATEHRAACLAAGIPFSTPFVALNRQCSSGLVAVNDIANKIKVGQIGIGLALGAESMSKNYGPKALGNVSAEMKQNKDAKKCMVPMGITNENVSHKFHVSRQSQDTFAAESHRKAERAISEGLFEDEILPINLPDGTTISTDEGPRKGVTADNLAQLKPAFIPEKGTTTAGNASQISDGVAAVLLARRSIAESMELPILGKYVACQVIGVPPEIMGVGPAYAIPKVLKDCGLAIEKVDVFEINEAFAGQALYCINKLQIPLAKVNPRGGAIALGHPLGCTGARQVATLLRELERGQVGIVSMCVGTGMGAAAVFVRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>C5DH23</accession>
    <name>C5DH23_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0E00770p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0E00770g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553521.1">
        <property type="nucleotide sequence ID" value="XM_002553475.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DH23"/>
    <dbReference type="FunCoup" id="C5DH23">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="GeneID" id="8291659"/>
    <dbReference type="KEGG" id="lth:KLTH0E00770g"/>
    <dbReference type="eggNOG" id="ENOG502S6Z8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_151392_1_1_1"/>
    <dbReference type="InParanoid" id="C5DH23"/>
    <dbReference type="OMA" id="NKEVGKW"/>
    <dbReference type="OrthoDB" id="16824at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013726">
        <property type="entry name" value="Mitofissin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28075">
        <property type="entry name" value="CHROMOSOME 16, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28075:SF1">
        <property type="entry name" value="DUF1748-DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08520">
        <property type="entry name" value="Mitofissin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950372" evidence="1">
        <location>
            <begin position="23"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="73" mass="7943" checksum="E59CE54D57C51E73" modified="2009-07-28" version="1">MTILGKAFHISFDLVLVSACLAGIKRNTGLTPKVDVIGDPHVKGYVSKYLNVGESCYDYTVATLGSSNYFVRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>C5DH64</accession>
    <name>C5DH64_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0E01694p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0E01694g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553562.1">
        <property type="nucleotide sequence ID" value="XM_002553516.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DH64"/>
    <dbReference type="FunCoup" id="C5DH64">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="GeneID" id="8291701"/>
    <dbReference type="KEGG" id="lth:KLTH0E01694g"/>
    <dbReference type="eggNOG" id="ENOG502S8TE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_144922_0_0_1"/>
    <dbReference type="InParanoid" id="C5DH64"/>
    <dbReference type="OMA" id="ICTMFAS"/>
    <dbReference type="OrthoDB" id="63581at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28008">
        <property type="entry name" value="DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G10980)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28008:SF1">
        <property type="entry name" value="DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G10980)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="128" mass="14100" checksum="C9ECAD368C18DD55" modified="2009-07-28" version="1">MPLINKKYFSTLLASSVLALFLGLTTDSKLTYFTSDHDKAAHFVVFFLESWLFTKSLIPRRIKLLSHTVDKYILSLLVCAVGAGVGSEFAQAVLSRGRRQFDSLDIVCNVCGGTLGVAVAGHAEFLWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>C5DH79</accession>
    <name>C5DH79_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 11">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="7 11">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0E02024g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7 10 11">Belongs to the peptidase C12 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="7 11"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553577.1">
        <property type="nucleotide sequence ID" value="XM_002553531.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DH79"/>
    <dbReference type="STRING" id="559295.C5DH79"/>
    <dbReference type="GeneID" id="8291716"/>
    <dbReference type="KEGG" id="lth:KLTH0E02024g"/>
    <dbReference type="eggNOG" id="KOG2778">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018316_1_0_1"/>
    <dbReference type="InParanoid" id="C5DH79"/>
    <dbReference type="OMA" id="YIQYEIQ"/>
    <dbReference type="OrthoDB" id="1924260at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.532.10">
        <property type="entry name" value="Peptidase C12, ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001578">
        <property type="entry name" value="Peptidase_C12_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036959">
        <property type="entry name" value="Peptidase_C12_UCH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017390">
        <property type="entry name" value="Ubiquitinyl_hydrolase_UCH37"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10589">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10589:SF16">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE ISOZYME L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01088">
        <property type="entry name" value="Peptidase_C12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038120">
        <property type="entry name" value="Ubiquitinyl_hydrolase_UCH37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00707">
        <property type="entry name" value="UBCTHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52048">
        <property type="entry name" value="UCH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7 10" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7 10" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="4 7 10" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UCH catalytic" evidence="12">
        <location>
            <begin position="2"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8 10">
        <location>
            <position position="86"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8 10">
        <location>
            <position position="169"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="10">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <feature type="site" description="Important for enzyme activity" evidence="9 10">
        <location>
            <position position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR038120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR038120-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR038120-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS52048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR23140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="319" mass="36311" checksum="7F472E5362AD0847" modified="2009-07-28" version="1">MSWNTIESDAGVFTQLIRDLGVSGVQFAEIPMLDSLEELAPSLYGVIFLFKYNSSDYAGNEPVQGAYDFDYPDSLFFANQTIQNACGTLAVLNTLLSLSNDFPEEIRLGDTLSEFISFTAAFKDPVLRGESVSNSDHIRNVHNSFSTPEPFVLDEKTPDDGRKAAEVFHFVSFVEHEGVLYELDGLRPTPIVHREYSNDSRDRLTFAQNIISVLQKRMQLCVNSGGKFSVIAIHRDAIERLEDLRQQGAITDQDYQLECSRELQKRETWAKELSMRRQNLYGLIFELLKQVSSSMTDEEFADHIKIAQKTTLERLKRRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHF4</accession>
    <name>C5DHF4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0E03916p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0E03916g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553652.1">
        <property type="nucleotide sequence ID" value="XM_002553606.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHF4"/>
    <dbReference type="FunCoup" id="C5DHF4">
        <property type="interactions" value="97"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHF4"/>
    <dbReference type="GeneID" id="8291801"/>
    <dbReference type="KEGG" id="lth:KLTH0E03916g"/>
    <dbReference type="eggNOG" id="KOG1842">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026440_0_0_1"/>
    <dbReference type="InParanoid" id="C5DHF4"/>
    <dbReference type="OMA" id="RKCGKLY"/>
    <dbReference type="OrthoDB" id="166134at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098588">
        <property type="term" value="C:bounding membrane of organelle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15761">
        <property type="entry name" value="FYVE1_Vac1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15737">
        <property type="entry name" value="FYVE2_Vac1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.20">
        <property type="entry name" value="Rabenosyn, Rab binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052727">
        <property type="entry name" value="Rab4/Rab5_effector"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021565">
        <property type="entry name" value="Rbsn_Rab-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036531">
        <property type="entry name" value="Rbsn_Rab-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13510">
        <property type="entry name" value="FYVE-FINGER-CONTAINING RAB5 EFFECTOR PROTEIN RABENOSYN-5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13510:SF44">
        <property type="entry name" value="RABENOSYN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11464">
        <property type="entry name" value="Rbsn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140125">
        <property type="entry name" value="Rabenosyn-5 Rab-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FYVE-type" evidence="7">
        <location>
            <begin position="233"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="427"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="452"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="427"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR23215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="519" mass="58811" checksum="D47834F62C8A112F" modified="2009-07-28" version="1">MNAVDGPGYLDEPQKSGSELNCPVCAQTLHDLAKLNEHLDRIHGFGEVSNEDADSVNTQLTRSRNVVENSSKAAKANRFELKRDHWIKPVMGKSRCRTCSKSLTQARQAINCRRCGEVYCKNHCNLIIKLNSNAEYNPLTGEWCKCCINCFGNRAGYNEFGAVCNKTSMFKELRGSKVEDKQLRKLQLENRFIRLANGIQQINNQSSKSMLASIRKYTEINKLERSVVPWTPDEAAFACSLCSKPFTLALRKHHCRLCGKVVCNESQTNCSNQLPLMSLIRAASDLPISTEFVDNNAEDVQIRICSACVRILFIKRKFTRELSLPEPSILRIYENMRTTADVILSILPQVLNQAEEFSATPNAPDVALLTKMRRKLLDSFALYDRLTKQMLKIKPKNPAEERIQRAIVFRASMLIQEHMLPLRSLTNAASTQNSSHETTPESERSMNQGYGNNLSIKEVKEYRDQLMVLKEQKFLVENMIASASKQRKFDEAETLSGNLRELNEQVEMIARALGEEGFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHM9</accession>
    <name>C5DHM9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glycerol kinase</fullName>
            <ecNumber evidence="3">2.7.1.30</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">ATP:glycerol 3-phosphotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0E05654g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the FGGY kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.30" evidence="3"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553727.1">
        <property type="nucleotide sequence ID" value="XM_002553681.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHM9"/>
    <dbReference type="FunCoup" id="C5DHM9">
        <property type="interactions" value="381"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHM9"/>
    <dbReference type="GeneID" id="8291880"/>
    <dbReference type="KEGG" id="lth:KLTH0E05654g"/>
    <dbReference type="eggNOG" id="KOG2517">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009281_2_2_1"/>
    <dbReference type="InParanoid" id="C5DHM9"/>
    <dbReference type="OMA" id="FMLMNIG"/>
    <dbReference type="OrthoDB" id="5422795at2759"/>
    <dbReference type="UniPathway" id="UPA00618">
        <property type="reaction ID" value="UER00672"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004370">
        <property type="term" value="F:glycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019563">
        <property type="term" value="P:glycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046167">
        <property type="term" value="P:glycerol-3-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006641">
        <property type="term" value="P:triglyceride metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000108">
        <property type="entry name" value="Glycerol kinase, glycosomal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018483">
        <property type="entry name" value="Carb_kinase_FGGY_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018485">
        <property type="entry name" value="FGGY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018484">
        <property type="entry name" value="FGGY_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005999">
        <property type="entry name" value="Glycerol_kin"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01311">
        <property type="entry name" value="glycerol_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10196:SF69">
        <property type="entry name" value="GLYCEROL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10196">
        <property type="entry name" value="SUGAR KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02782">
        <property type="entry name" value="FGGY_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00370">
        <property type="entry name" value="FGGY_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00933">
        <property type="entry name" value="FGGY_KINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00445">
        <property type="entry name" value="FGGY_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0319">Glycerol metabolism</keyword>
    <keyword evidence="6 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase FGGY N-terminal" evidence="12">
        <location>
            <begin position="123"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate kinase FGGY C-terminal" evidence="13">
        <location>
            <begin position="382"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="48"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00043149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR23290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="686" mass="75010" checksum="C623F43C6191359D" modified="2009-07-28" version="1">MTAAAPTGATEEPSEVPLVASIDVGTTSSRVILFNMLGQEVSKHQIEYSTSASREKYAPVGKRRHSVDETTAAAAGGGSDSRRASRASPPSPAQPIFSAEGIAIEANEYLEIEDLERPSVPTLSFPRAGWVECNPLGILAHVVQCFAANLVSVDRVNGSRVSNGLAPYRIRCVGIANMRETTLVWSRKTGEPVVPYGIVWNDTRTSAIVTEKRNSTPQAVQDRLRAKTGLPLYSTYFSCSKLRWLLDNEPAVRACYDAGDLMFGTVDTWLIYHLTSNRAFVSDVTNASRTGFMNLDTLVYDDELLNYWDLARDKVNLPRIVSSAEYYGDFALPECVRTNLTPVAWSLLTDFCARTPAVPIQGCLGDQSASLVGQLAFKPGAAKCTYGTGCFLLYNTGTKKLISSHGALTTFGYWFPHLENEEHAQPHFALEGSVAVAGAVVQWLRDNLRLIPKAEDIGPLASRVSDTGGVVFVPAFSGLFAPYWDASARATIMGMSQHTTASHLARAAVEGVCFQARAILKAMSSDAAGEGRKDRDFLEEVADGTYEKAPLSMLAVDGGMSRSKEVMQIQADILGPCVKVRTSASSECTALGAAIAAALAYEKREDRVLWKDLKDVKKWVMYGGTEPGNRPPVDEESFRTRIFRSKSIDTERRRKWKLWEAAIERSRGWMNIVEQSTHDNATAKNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-04-02" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHQ0</accession>
    <name>C5DHQ0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0E06116p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0E06116g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553748.1">
        <property type="nucleotide sequence ID" value="XM_002553702.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHQ0"/>
    <dbReference type="GeneID" id="8291901"/>
    <dbReference type="KEGG" id="lth:KLTH0E06116g"/>
    <dbReference type="eggNOG" id="ENOG502SRFB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_339491_0_0_1"/>
    <dbReference type="InParanoid" id="C5DHQ0"/>
    <dbReference type="OMA" id="CVYELPP"/>
    <dbReference type="OrthoDB" id="4034754at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050613">
        <property type="entry name" value="Sec_Metabolite_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001:SF88">
        <property type="entry name" value="TRANSCRIPTION FACTOR PDR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001">
        <property type="entry name" value="UNCHARACTERIZED TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="3">
        <location>
            <begin position="20"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR23311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="837" mass="93597" checksum="E1094A258EBB7A62" modified="2009-07-28" version="1">MTSNPPAVKAPREKPAAMFTCKSCRQRKRKCSREKPACQRCVRLAIPCVYELPPKHLIRSVSTGTIEGVGADSAGGAGSAGSASASASTSVSVDVSGRSSACSTAGAAGSGLLRDFPAGFMHGSALASLPTLEPTGSLDTDTRISGQPLKVHFHTTLDLDSHLHKDPFLLEFAASCVACGPQLARNPQSSGLGHAWFSLTVQCHDLLVPRLNSLKTPSPVPSLLAPGTPRTRAFQGPRSEDFQASCAALIADISSALPSMQAIETYKVQFYQNVYTAVPVLNIQTFERSFQDLVVGDSCGAARLKFGSADHHPKITHLVILLMAIRMGYVCLCLYNDRVERDALLKKQTELWLRQNAVPRDVLLLVKRCLELLDVYENPSEDILCCLIYLRQILLLERSDGWSLIGVTNAELLEATVYLASRMNLYSNAIDTMTEGATTVQEKLYRRKLWLSICSISLHEHTLRGGEPFVKARQLRQFSDHYQVFDDYRAISLRELPLDDQIELDYHIMLLKNHQFMEVLCDIEESHPSNVEFLDNKVHENERLALFFKDGFPDADPAYKIDVNLPLYLLCNSGHIPVRIGSVKQIAVFQTRFALLIKQLSNSSLLMFYYEKEYKFSNAVDSLYNFERHLFDSMKILLQLFEYYRDYAFGRLAEVTPSAMRYILANTMSPIFNRTTLVIHGIILRFVYLQERADFERDLEKAPLLQKIVSQLRALVVTSSDCMKMEFLPPKSLDLNRNFIRLLDARVLLISTKQYAVLDDIDLEKQHDAMQICGPGYADYYNNAVTQLSLNSLGKFLQLLESTTFNKKSDSPMNIFSNIASYSGASLDANENTSSFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHU9</accession>
    <name>C5DHU9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 12">Mannosyl-oligosaccharide glucosidase</fullName>
            <ecNumber evidence="11 12">3.2.1.106</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Glucosidase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">KLTH0E07304g</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Cleaves the distal alpha 1,2-linked glucose residue from the Glc(3)Man(9)GlcNAc(2) oligosaccharide precursor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N(4)-(alpha-D-Glc-(1-&gt;2)-alpha-D-Glc-(1-&gt;3)-alpha-D-Glc-(1-&gt;3)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] + H2O = N(4)-(alpha-D-Glc-(1-&gt;3)-alpha-D-Glc-(1-&gt;3)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] + beta-D-glucose</text>
            <dbReference type="Rhea" id="RHEA:55988"/>
            <dbReference type="Rhea" id="RHEA-COMP:12806"/>
            <dbReference type="Rhea" id="RHEA-COMP:14355"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15903"/>
            <dbReference type="ChEBI" id="CHEBI:59082"/>
            <dbReference type="ChEBI" id="CHEBI:132537"/>
            <dbReference type="EC" id="3.2.1.106"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="13">Glycan metabolism; N-glycan degradation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Endoplasmic reticulum membrane</location>
            <topology evidence="1 12">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the glycosyl hydrolase 63 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.106" evidence="11 12"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553797.1">
        <property type="nucleotide sequence ID" value="XM_002553751.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHU9"/>
    <dbReference type="FunCoup" id="C5DHU9">
        <property type="interactions" value="628"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHU9"/>
    <dbReference type="GeneID" id="8291953"/>
    <dbReference type="KEGG" id="lth:KLTH0E07304g"/>
    <dbReference type="eggNOG" id="KOG2161">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007380_2_0_1"/>
    <dbReference type="InParanoid" id="C5DHU9"/>
    <dbReference type="OMA" id="FNWYNTT"/>
    <dbReference type="OrthoDB" id="410058at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004573">
        <property type="term" value="F:Glc3Man9GlcNAc2 oligosaccharide glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009311">
        <property type="term" value="P:oligosaccharide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.110">
        <property type="entry name" value="Glycosyl hydrolase family 63, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031335">
        <property type="entry name" value="Glyco_hydro_63_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031631">
        <property type="entry name" value="Glyco_hydro_63N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038518">
        <property type="entry name" value="Glyco_hydro_63N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004888">
        <property type="entry name" value="Glycoside_hydrolase_63"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10412">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10412:SF11">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03200">
        <property type="entry name" value="Glyco_hydro_63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16923">
        <property type="entry name" value="Glyco_hydro_63N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 12" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002947847" description="Mannosyl-oligosaccharide glucosidase" evidence="14">
        <location>
            <begin position="19"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 63 N-terminal" evidence="16">
        <location>
            <begin position="38"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 63 C-terminal" evidence="15">
        <location>
            <begin position="305"/>
            <end position="798"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU368089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU369107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF03200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF16923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAR23360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="804" mass="91617" checksum="69E00FE7A47142D8" modified="2009-07-28" version="1">MLLRLLVVFALTLFEAHAETQGLFSDDVEEFERSTNQSLLWAPYRSNCYFGVRPRFINENPLMMGLMWFDASHAQGISSLRHFVEQNDRLEKYGYELYDPRIGGKEAIIDRENNLNLTIHFVKSKDGENWAVRVSGSPLDSSKLSASSLVVYLSQNGPGGRLESEHGKTSTEKRIELKGSAPELGDYEITIEDISGQHYKRGPALGPDIDGSKTSHMSLTVPGDQVWKARDIFQSLLTDSIQNIITSGIPMDALSIPSALMIRNIHNFPPGNFHFVQKTFDLAEPFEFDIIFNNKASRQPIGSSAEVSNLISWSTNELKARFDSKFDIKDSSLKQFAQETLANLLGGIGYFHGTQLVDRSTEFDEEQFNKIELRAPREEGPIQLFSSVPSRAFFPRGFYWDEGFHMLQVMEYDFDLVLEILQSWFLLIEDDSGWVGRELILGSEARSKVPEEFQVQNPNIANPPTLLLCFSEMLTRAQEFQDSIAGGAEESSITPSSTEELLKRPELLMSFAEKVYPKLLKHYEWWTGSQRGFTEEYSEVLQDGFHPNEAFRWVGRTFTHCLPSGLDDYPRAQPPDVAELHLDALSWAGIMSRSMKQIADVLNMADEVDKFSKIEQNIIENLDTIHWNADQKCYCDVTIDDDFDELRRFVCHEGYVTLLPFALKLVPRDSDKLQHYVELMGDTDKLFSDFGVLSLSKQDEYFETGEAYWRGPVWINMNYLLLDSLLYYFQGGGENQDTATAERARAVYSDLRKNLIANMHRVWSEKGYVYENYNQRTGNGSGVQHFTGWSALIVNIMGRLPVTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIH3</accession>
    <name>C5DIH3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0E12518p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0E12518g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002553975.1"/>
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    <dbReference type="STRING" id="559295.C5DIH3"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014957_0_1_1"/>
    <dbReference type="InParanoid" id="C5DIH3"/>
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        <property type="component" value="Chromosome E"/>
    </dbReference>
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        <property type="term" value="C:catalytic complex"/>
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        <property type="term" value="C:cell cortex"/>
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        <property type="term" value="C:site of polarized growth"/>
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        <property type="term" value="F:molecular adaptor activity"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="F:phosphatidylinositol binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:positive regulation of cellular component organization"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="PX_Bem1p"/>
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        <property type="entry name" value="SH3_Bem1p_1"/>
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        <property type="entry name" value="SH3_Bem1p_2"/>
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        <property type="entry name" value="Protein kinase activator Bem1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
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        <property type="entry name" value="NADPH_Oxidase/PX-Domain"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
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    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR15706:SF2">
        <property type="entry name" value="SH3 AND PX DOMAIN-CONTAINING PROTEIN 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15706">
        <property type="entry name" value="SH3 MULTIPLE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="61"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="152"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="PX" evidence="6">
        <location>
            <begin position="281"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="PB1" evidence="7">
        <location>
            <begin position="479"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="423"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="15"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="437"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR23584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="552" mass="62055" checksum="2355B54F9C5703B4" modified="2009-07-28" version="1">MLKGFKRSGRDSGTKSRITSADISAPTADNNNVVKHIKTVPLRSASSASNNSGKGPDKIMPVEKVIKALYNYQAQSPKELSFVKGEFFVVQAEDKEWYDAFNPQDQRRGMVPKSYFESFARGGPEFNNGNGVPLTQHQQQLPVNSSFISQRSGSLYAIVLYDFRAEKSDELTTYAGENLFICAHHNYEWFIAKPIGRLGGPGLVPVGFVSIIDIDTGYATGNETSEDIASVNLPTVQEWKNNVAKYKASNISLSSPEIQKQRSNSHGSSLQQQYLPSRSSYLNSAITHAAVDSFSLEGDKFWFNVICELPDGRTRSLKRYYEDFYDLQVKLLDAFPAEAGKLRDVRGQWTKRIMPYIPGPVPYVTDSITKKRKEDLNIYVRDLIALPMHISQCDMVRSLFTIRNNGFDKEFTQEPTMTLNQFPATYEDPQHSRAGSRKNDDSTLTGDDLKVYEMMNDLSLKNSKPHSRPPSALPPQLKPTKIKFYYKDDIFALLLDANITFIELRDKISPRIESPSFKLFVRLNDSLGEEVTGDAQVSEVIQGKLKIAVQDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIL1</accession>
    <name>C5DIL1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0E13376p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0E13376g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MGR2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554059.1">
        <property type="nucleotide sequence ID" value="XM_002554013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIL1"/>
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        <property type="interactions" value="266"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIL1"/>
    <dbReference type="GeneID" id="8292228"/>
    <dbReference type="KEGG" id="lth:KLTH0E13376g"/>
    <dbReference type="eggNOG" id="KOG4096">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_142435_0_0_1"/>
    <dbReference type="InParanoid" id="C5DIL1"/>
    <dbReference type="OMA" id="PSNWEKF"/>
    <dbReference type="OrthoDB" id="5409308at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018450">
        <property type="entry name" value="Romo1/Mgr2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28525">
        <property type="entry name" value="REACTIVE OXYGEN SPECIES MODULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28525:SF1">
        <property type="entry name" value="REACTIVE OXYGEN SPECIES MODULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10247">
        <property type="entry name" value="Romo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01378">
        <property type="entry name" value="Romo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="56"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR23622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="127" mass="13963" checksum="2922A2AC1054903C" modified="2009-07-28" version="1">MPPVKQVYSQQQPSNWEKFKMGLMMGTSVGVCTGILFGGFAILTQGPGPNGYIRTLGQYIAGSAATFGLFMSIGSIIRSEELAPVGTITEGNLNTLQRRAKFEAWRAQAAYGLYRKRATELKEFKDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIM8</accession>
    <name>C5DIM8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0E13750p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0E13750g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554076.1">
        <property type="nucleotide sequence ID" value="XM_002554030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIM8"/>
    <dbReference type="FunCoup" id="C5DIM8">
        <property type="interactions" value="35"/>
    </dbReference>
    <dbReference type="GeneID" id="8292245"/>
    <dbReference type="KEGG" id="lth:KLTH0E13750g"/>
    <dbReference type="eggNOG" id="ENOG502S12S">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125620_0_0_1"/>
    <dbReference type="InParanoid" id="C5DIM8"/>
    <dbReference type="OMA" id="EYMIERC"/>
    <dbReference type="OrthoDB" id="4082971at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="180" mass="20690" checksum="83729786CA9E109F" modified="2009-07-28" version="1">MLRNQVYKQTSMIARDFAKLQISVPTAADGEKYSSTASGRPNQSVTKPVSLDSSTGEVLVRKSTGKTKVRKGQTAEEFERQCAQFFDVERGPVWTPVGWMTTRDPLLTLDTDPSSDLKIKQVRQKLASHCHLLYYRKQYADCARLCERLLSRFEALDNRKKIQREIDELHYMLERCRAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIT1</accession>
    <name>C5DIT1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0E14938p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0E14938g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554129.1">
        <property type="nucleotide sequence ID" value="XM_002554083.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIT1"/>
    <dbReference type="GeneID" id="8292299"/>
    <dbReference type="KEGG" id="lth:KLTH0E14938g"/>
    <dbReference type="HOGENOM" id="CLU_637892_0_0_1"/>
    <dbReference type="InParanoid" id="C5DIT1"/>
    <dbReference type="OrthoDB" id="4033174at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="273"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="224"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="333"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="41"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR23692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="430" mass="49391" checksum="647CBE4765AF2FDE" modified="2009-07-28" version="1">MESGKRQSINSPARPAPRETSCSETLLNDGDDDAQANSTEIDNHDMETGRDVSPSPRNLVGTTSHDEMQQEQLYLEYFHSAIITEELGLIKHLKRAHGKMIKAIQELIRVKGYRSADSGKEYRFKWTDNATYYLLKYCLEIGPFYGFEHLKRPSLTKLEQSSLISLKWSCVWYDLLMTLKFENSIIPTESQIKSKCRYLLDNAHPKLFAVTTGDERNPFSFTGVDKVDNILEMLTRLKNDAEQRKVEIQKLETDRQNNKRTLGQMLCSNSIPSVTEGLSNSDSEEPSSIDDINQPNSITMRRNKRAGRLLNFKEIAQYSLSNSALVGDLQGTLKESIEAAAVEERKYKDKEQQRKDQELALKQKKLEISLQHNQHMLELELERQKREQFDHDAKKIDTLLKMMDRAKSLEGDKSPSSEALQKLLHNFVSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJ18</accession>
    <name>C5DJ18_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0E17138p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0E17138g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554216.1">
        <property type="nucleotide sequence ID" value="XM_002554170.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJ18"/>
    <dbReference type="STRING" id="559295.C5DJ18"/>
    <dbReference type="GeneID" id="8292398"/>
    <dbReference type="KEGG" id="lth:KLTH0E17138g"/>
    <dbReference type="eggNOG" id="KOG0065">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_35_2_1"/>
    <dbReference type="InParanoid" id="C5DJ18"/>
    <dbReference type="OMA" id="PQITEGY"/>
    <dbReference type="OrthoDB" id="245989at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19241">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="133"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="172"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="223"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="254"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="374"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="ABC-2 type transporter transmembrane" evidence="7">
        <location>
            <begin position="83"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR23779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="404" mass="46336" checksum="96718C6AC9D379AD" modified="2009-07-28" version="1">MIDYFEKNGAHQCPKDVNPAEWMLEVIGAAPGSHADQDYHEVWKASKECKATQAELEWMEKELVKKSQDNFERGEFASSLLFQYFFVTKRLFQQYWRTPSYLWSKAILTLFSQIFIGFTFFKADRSLQGLQNQMLSVFMFTVVFNPTVQQYLPTYISQRDLYEARERPSKTFSWIAFILSQITVEIPWNFTIGTLGFSCYYYPVSFYRNASFANQLHERGALIWLFCTAFYVFTGSMAQLCVASQEVAQSAGHIASLLFVLSLSFCGVMVTPNNMPGFWKFMYRVSPLTYFIDGVLSTGIANSKVECSDYEFVTFTPRSGQNCGEYMALYIDAAGTGYIKDSGATVKCSFCPASSTNAFLKMVSSNYSHRWRNYGIFLCYICFNIFASVFLYWLARVPKRKAGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJ21</accession>
    <name>C5DJ21_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0F12826p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0F12826g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein disulfide isomerase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554747.1">
        <property type="nucleotide sequence ID" value="XM_002554701.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJ21"/>
    <dbReference type="FunCoup" id="C5DJ21">
        <property type="interactions" value="129"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJ21"/>
    <dbReference type="GeneID" id="8292970"/>
    <dbReference type="KEGG" id="lth:KLTH0F12826g"/>
    <dbReference type="eggNOG" id="KOG0191">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024937_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJ21"/>
    <dbReference type="OMA" id="RQVNCVE"/>
    <dbReference type="OrthoDB" id="72053at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02961">
        <property type="entry name" value="PDI_a_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051063">
        <property type="entry name" value="PDI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45672">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE C17H9.14C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45672:SF3">
        <property type="entry name" value="THIOREDOXIN DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="674"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="4">
        <location>
            <begin position="40"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR24310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="726" mass="83180" checksum="8FDCB9EA73E6D6E5" modified="2009-07-28" version="1">MKLLRIGFLQVRITALHKNETLIKGAVKMRLKQLLLSLVLCTRLVVPLDDAESVDEDPFPQPVSAAGFREAISKHLQLVEFYSPFCHHCKKLAPVWEKTWKSFREEGEKLNISLIQVNCVENGDLCAEERIQAFPSIKLYGPSGFIKDFPNSWKRNEKTISNFARQEAMNLDNFDTSLLYSKSKSLDGPEFLELIAGNGNLPNLVSFWPSEDLKNVDDNSFEFENCDECHGFQKIWTLLSNKLEPEGFVTSHFNCAANKKICQELGLENLAKLTNHRSDRKPQVALVLPNKTVNNLFFYHKYDDSVQSYADFATRTYSNAQIPETNMKSLGQLIEEPLELKKGQKSSDANIYVVFKYESETVVQEDFEILEQLIEPISRIPNAFLYKSSDDMMGLSRRLFNQMYNVINYEDNEEQKKPNEEFFVMSTLTQYPTFFLFKQGNLVPTVFHGYSTTEMRNLNFILKWLSENSLPLLNEVTSENFDSLLGYHSELYNTMAVQLLDTSSESEYSRSMTYVENFLTSAFDYEATRSDFLYETVLNKRLMKEEAVRSLKNKNAPSTEIVKKMRDEIVHNDDHRALFAYLDLASHKALLTQAGLNAYDRTYKNGDVLIIDKVTGSYYYDRDQVGNMLTTTSPSQLKNTLARLNFPTKFSRSALAKIPIQNLNRGLTSILRPVLHPGLAGYALACVAFLFAFLRISKAYRKSVIGRKYRSKRDVTGILGKPKPIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJ24</accession>
    <name>C5DJ24_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0F12892p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0F12892g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554750.1">
        <property type="nucleotide sequence ID" value="XM_002554704.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJ24"/>
    <dbReference type="FunCoup" id="C5DJ24">
        <property type="interactions" value="46"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJ24"/>
    <dbReference type="GeneID" id="8292973"/>
    <dbReference type="KEGG" id="lth:KLTH0F12892g"/>
    <dbReference type="eggNOG" id="ENOG502S95F">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_855219_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJ24"/>
    <dbReference type="OMA" id="HICITEP"/>
    <dbReference type="OrthoDB" id="4036527at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="184"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="238"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="323" mass="36287" checksum="11E5E622BC587B53" modified="2009-07-28" version="1">MRSIRKSGESTCPKKRRNYQFISIKQTSSFEALKTLPGLAPSKQLTPDAIFSVVQRELKQVKLDSLKINECVLHDISSEAAQTDKIEHQLQVSMRKLHRHYRRIIKRREVLENSTSRNIECCSAAVTKIEVLLESLKELSDPLVSLIYKKDARLPLKNRLSSDQGINRQHYPLLFEALKKYESNHKSQKNNQSAIPAQSRAPSCKSDTDLSALKEAPGQKNEASCEPKSGPESATPPCENNNSLHSSETSPVPEISSFTNQHTHLGTAKASIPEFMLMPQFRKASRASVITTYEQACHPCQVEDQEVFGKLAGSLHKNLDIRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJD0</accession>
    <name>C5DJD0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0F15444p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0F15444g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554856.1">
        <property type="nucleotide sequence ID" value="XM_002554810.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJD0"/>
    <dbReference type="FunCoup" id="C5DJD0">
        <property type="interactions" value="18"/>
    </dbReference>
    <dbReference type="GeneID" id="8293087"/>
    <dbReference type="KEGG" id="lth:KLTH0F15444g"/>
    <dbReference type="eggNOG" id="ENOG502S9CE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_164897_1_0_1"/>
    <dbReference type="InParanoid" id="C5DJD0"/>
    <dbReference type="OMA" id="AQVKPGF"/>
    <dbReference type="OrthoDB" id="3999982at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="104" mass="11894" checksum="972AC139E7A341B6" modified="2009-07-28" version="1">MPSPNPFQNIGRNLLYLIGGLAVSVYMTKKLTRANKVTQFDRMQEKAKNEEEYYKNLAHVKPGFPISADDSKETRKSEFEGSGLSYLSRKGGDRLGFFDRRNKD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-06-28" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJH5</accession>
    <name>BL1S4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName>Biogenesis of lysosome-related organelles complex 1 subunit CNL1</fullName>
            <shortName>BLOC-1 subunit CNL1</shortName>
        </recommendedName>
        <alternativeName>
            <fullName>CNO-like protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">CLN1</name>
        <name type="ordered locus">KLTH0F16478g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the biogenesis of lysosome-related organelles complex-1 (BLOC-1), a complex that is involved in endosomal cargo sorting.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the biogenesis of lysosome-related organelles complex-1 (BLOC-1).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="1">Punctate pattern.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the BLOC1S4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJH5"/>
    <dbReference type="SMR" id="C5DJH5"/>
    <dbReference type="FunCoup" id="C5DJH5">
        <property type="interactions" value="36"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJH5"/>
    <dbReference type="KEGG" id="lth:KLTH0F16478g"/>
    <dbReference type="eggNOG" id="ENOG502S4DQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_141728_1_0_1"/>
    <dbReference type="InParanoid" id="C5DJH5"/>
    <dbReference type="OMA" id="HFDMLDQ"/>
    <dbReference type="OrthoDB" id="5424991at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031083">
        <property type="term" value="C:BLOC-1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd24144">
        <property type="entry name" value="BLOC1_CNL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034455">
        <property type="entry name" value="CNL1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39145">
        <property type="entry name" value="BIOGENESIS OF LYSOSOME-RELATED ORGANELLES COMPLEX 1 SUBUNIT CNL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39145:SF1">
        <property type="entry name" value="BIOGENESIS OF LYSOSOME-RELATED ORGANELLES COMPLEX 1 SUBUNIT CNL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0175">Coiled coil</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000410644" description="Biogenesis of lysosome-related organelles complex 1 subunit CNL1">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="73"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="131" mass="15183" checksum="CB36296D13C4F5A4" modified="2009-07-28" version="1">MSAPDSNSGHAHDSAQNEGAAEGTRDPFGIDRLSVDYDYLLYRIQDHVTSIQLSTTEICRQQNQLVEQGIIGDAIDINIEEMRRILQKCEELETHFDMLDQIDSIVQTFRPRLDDIVREHRELTRNTERRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJL1</accession>
    <name>C5DJL1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0F17314p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0F17314g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HFCD (homooligomeric flavin containing Cys decarboxylase) superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554937.1">
        <property type="nucleotide sequence ID" value="XM_002554891.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJL1"/>
    <dbReference type="FunCoup" id="C5DJL1">
        <property type="interactions" value="66"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJL1"/>
    <dbReference type="GeneID" id="8293171"/>
    <dbReference type="KEGG" id="lth:KLTH0F17314g"/>
    <dbReference type="eggNOG" id="KOG0672">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014402_1_0_1"/>
    <dbReference type="InParanoid" id="C5DJL1"/>
    <dbReference type="OMA" id="WNPSYPI"/>
    <dbReference type="OrthoDB" id="1532798at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071513">
        <property type="term" value="C:phosphopantothenoylcysteine decarboxylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004633">
        <property type="term" value="F:phosphopantothenoylcysteine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1950">
        <property type="entry name" value="Flavin prenyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036551">
        <property type="entry name" value="Flavin_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003382">
        <property type="entry name" value="Flavoprotein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14359">
        <property type="entry name" value="HOMO-OLIGOMERIC FLAVIN CONTAINING CYS DECARBOXYLASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14359:SF17">
        <property type="entry name" value="PHOSPHOPANTOTHENOYLCYSTEINE DECARBOXYLASE SUBUNIT SIS2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02441">
        <property type="entry name" value="Flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52507">
        <property type="entry name" value="Homo-oligomeric flavin-containing Cys decarboxylases, HFCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavoprotein" evidence="3">
        <location>
            <begin position="316"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="100"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="364"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="564"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="100"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="195"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="214"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="232"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="296"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="374"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="568"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="583"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="596"/>
            <end position="630"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR24500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="658" mass="70423" checksum="CAE85F1D25FA1AC7" modified="2009-07-28" version="1">MSEQNTNSEKTPGIVSPRFSNAANKANPDGSMGPPPVVAPGANSPVSQCSASPGTCATKSIMNVSGTSGAVVSNTPEPGLKRVPTVTFSDSKHLTLKQQAVANAANQQAKTSGAEENDKGDATSPKAKPVTSPSAGIPNSGSRLPSPPPLTSTDSTASAQRQGTAFGGAGRHDGKKGNIISKLLHSADILHHSSSEQNTSGEEAPRSSSVTSEPEPKKDDHPHFVVDDALHSPSFRSRSNSASPMPQVPSPSFTLGEKRQDSALRQPEATIAESLDESGELPSEASNFHQIAPNIPKRETGKNSEPRLPQDDGKLHVLFGATGSLSVLKIKAMIKKLEEIYGRDKISIQLILTQAAAQFFANRSRKPRIESRTASESTAPTMSSNPAAGEQAKPLATAPSAAAHNHNVTVGGGPPGGTPTMAPKIELPAHIQVWTDQDEWDVWKQRTDPVLHIELRRWADILVVAPLTANTLSKVALGLCDNLLTSVVRAWNPMFPIFLAPSMVSSSYNSTITKRHLKMIKEEMPWITVFKPSEKVMGIHGDIGLGGMMDANEIVDKVVMKLGGYPKDEEEEDDDEEEEDDDSKSARQKEERRADGEEDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDVPNPTAKIPLQNSANSGVSHPELVPQISR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJN0</accession>
    <name>C5DJN0_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Methylenetetrahydrofolate dehydrogenase [NAD(+)]</fullName>
            <ecNumber evidence="12">1.5.1.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">KLTH0F17754g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes oxidation of cytoplasmic one-carbon units for purine biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.15" evidence="12"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554956.1">
        <property type="nucleotide sequence ID" value="XM_002554910.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJN0"/>
    <dbReference type="FunCoup" id="C5DJN0">
        <property type="interactions" value="307"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJN0"/>
    <dbReference type="GeneID" id="8293191"/>
    <dbReference type="KEGG" id="lth:KLTH0F17754g"/>
    <dbReference type="eggNOG" id="KOG0089">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031413_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJN0"/>
    <dbReference type="OMA" id="CKVITAE"/>
    <dbReference type="OrthoDB" id="41403at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004487">
        <property type="term" value="F:methylenetetrahydrofolate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004488">
        <property type="term" value="F:methylenetetrahydrofolate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01079">
        <property type="entry name" value="NAD_bind_m-THF_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000255">
        <property type="entry name" value="Methylenetetrahydrofolate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10860:FF:000012">
        <property type="entry name" value="Methylenetetrahydrofolate dehydrogenase [NAD(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035812">
        <property type="entry name" value="m-THF_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000672">
        <property type="entry name" value="THF_DH/CycHdrlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020630">
        <property type="entry name" value="THF_DH/CycHdrlase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020631">
        <property type="entry name" value="THF_DH/CycHdrlase_NAD-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099">
        <property type="entry name" value="C-1-TETRAHYDROFOLATE SYNTHASE, CYTOPLASMIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099:SF3">
        <property type="entry name" value="METHYLENETETRAHYDROFOLATE DEHYDROGENASE [NAD(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00763">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02882">
        <property type="entry name" value="THF_DHG_CYH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00085">
        <property type="entry name" value="THFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tetrahydrofolate dehydrogenase/cyclohydrolase catalytic" evidence="14">
        <location>
            <begin position="11"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Tetrahydrofolate dehydrogenase/cyclohydrolase NAD(P)-binding" evidence="15">
        <location>
            <begin position="149"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00053076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00061364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00066980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00074830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAR24519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="320" mass="36272" checksum="C584CA0DE1B924D1" modified="2009-07-28" version="1">MSTKPGRTILASSVSNTYADEITSKVESLRKERPNGPLLVGFLANNDPAAEMYANWTSKTCESMGFRYELRRVEEKDFLEEAIIEANRDNAVDGIMVYFPVFGNAQDQYLQQVVSREKDVEGLNHVYYQNMYHNIRYLDDEQQLKSILPCTPLAVVKIMEYLKIYNSLLPQGNRLYGKKCVVVNRSEIVGRPLSALLANDGAIVYSVDINNIQKFTRGEGLKFQKHQVEDLGPFSNEMLKECCADADVIITGVPSESYKFPTEYIKEGAVCINFSSHKNFDESVKSKASLYVPSTGKVTISMLLRNMLRLVENRQKMNLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJS7</accession>
    <name>C5DJS7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0F18810p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0F18810g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555003.1">
        <property type="nucleotide sequence ID" value="XM_002554957.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJS7"/>
    <dbReference type="FunCoup" id="C5DJS7">
        <property type="interactions" value="145"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJS7"/>
    <dbReference type="GeneID" id="8293239"/>
    <dbReference type="KEGG" id="lth:KLTH0F18810g"/>
    <dbReference type="eggNOG" id="KOG0087">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030444_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJS7"/>
    <dbReference type="OMA" id="WHQVVER"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001447">
        <property type="entry name" value="Ras-related protein Rab-1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="173"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="359"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="412"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="368"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR24566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="448" mass="49196" checksum="272812E3BD5F4B3E" modified="2009-07-28" version="1">MSDSRRKRYSINFSAPASPASPSLGAFPFAEQAPAVRPASARAHSARPSSAYGLIGGASSGGSAATAATAAGQSFYRIPNDDQRNVSLDSLADSSSIKLLLIGDATVGKTAMILSYCNELPTRSQVSLFAKDSARAPSLQARRHKRIQNLKTIEKRKRYSLNDYEELFTHPAAARDAPSLSEDPDAAGLSPDAPPREDPNEIIIDTRSTIGVDIRTNLVNIDHRYFKVTMWDTAGQERYRNAMIPSLYKGSHGILLSYDICSRKSFHNCLDYWLQEVLNCCAGDKKLRLYLVGNKIDLYKVRQVTHEDVLRLIAEAEQKHGVKIAGNFEVSCKWHQVVERTFNIIIKDLAEHGCYEDDLHRHGDSPRPTHTRASTSTRTPTPTPTQAQAQAHSCDSCGPVAIPADVPMEIERRGSTIVRRRSKEPPKTIDISKPLDPGDSSGRAACCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJT2</accession>
    <name>C5DJT2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit protein uS11m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0F18920g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uS11 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555008.1">
        <property type="nucleotide sequence ID" value="XM_002554962.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJT2"/>
    <dbReference type="FunCoup" id="C5DJT2">
        <property type="interactions" value="235"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJT2"/>
    <dbReference type="GeneID" id="8293244"/>
    <dbReference type="KEGG" id="lth:KLTH0F18920g"/>
    <dbReference type="eggNOG" id="ENOG502S752">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072439_4_0_1"/>
    <dbReference type="InParanoid" id="C5DJT2"/>
    <dbReference type="OMA" id="FHRHNTL"/>
    <dbReference type="OrthoDB" id="1654884at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.80:FF:000011">
        <property type="entry name" value="37S ribosomal protein S18, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.80">
        <property type="entry name" value="Ribosomal protein S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01310">
        <property type="entry name" value="Ribosomal_uS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001971">
        <property type="entry name" value="Ribosomal_uS11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036967">
        <property type="entry name" value="Ribosomal_uS11_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11759">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S14/30S RIBOSOMAL PROTEIN S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00070326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR24571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="207" mass="23028" checksum="009766485F07954F" modified="2009-07-28" version="1">MIKSVFSTACRPFSTSARALNESPVSFAGIAQAINASNPVSNVISPGEEPGSKSSKKRGEHTVKYILNCLFTKNNTHFTYSAVVEDLNYLKNNADASYNDKFLYYLKLPQKVKFRVSTGNLGFRKAARGEYEAAFQTSARLFQMIHQKKLLDRNIEIVLKDFGKGREAFTAALNGKEGTAVRRNVVRVSDATPIKFGGVRAPRQRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJT9</accession>
    <name>C5DJT9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0F19074p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0F19074g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555015.1">
        <property type="nucleotide sequence ID" value="XM_002554969.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJT9"/>
    <dbReference type="FunCoup" id="C5DJT9">
        <property type="interactions" value="94"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJT9"/>
    <dbReference type="GeneID" id="8293251"/>
    <dbReference type="KEGG" id="lth:KLTH0F19074g"/>
    <dbReference type="eggNOG" id="ENOG502QTPK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_409412_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJT9"/>
    <dbReference type="OMA" id="FKIPLWY"/>
    <dbReference type="OrthoDB" id="4032719at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="46"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="180"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="210"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR24578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="729" mass="83766" checksum="CCE86072C017ADFE" modified="2009-07-28" version="1">MKRLQLFGRGKVDSRESYRPLPSTFKSNAKSDTSQRVSNDLKRNEASGLLQLPSKVLDQILNLLDTQTLLSLCLVHSKLYNIISNNFLYERIVLKDKVALLKFAALIHCESHTIDALTQEREGSVSQNLRFLVRSLEFVNPSYQDSLLKYSKFCGKDQSTTIGGSYRISNVSTIKDMQGLARGRSGEPNSHAGSSGVSRERSHSSESRSWQSTNRSPSSLREKTYTPLGSFFESSSTSSISRMIEKYENHTYIELMLDIIDFCPNVTSIILSDIQHGFKVPLWYSVLNDGSKDFFKRIIKGQQSMNRDDLMSFQLSSQWISDYEEKYHTFPRFKRLELRGCDDKPPVILRSNMLSCFGVFEELSLKNMALDAESLDTPFEYVPFYMKIGDKGLLDLHFPVTTLLIDRCEIIPGNGIMKLMCSYFHRVKNLKLLSLRSKYDLILTRCFPNLAHVTIDCNSNCFKYERVVGDAYYHKEVNCDLPDDRSVAETLVDSTVTQELIIPPATTPIVLAMNGGYIKRSSGVGGRKPGIISELQEQYFTNMKVPPFHYFYHYYKSIWDRIPNRNVSIKIVNIPFTNVFPLPPDIYWRQLESYADDDSQTLCGNSRNLEANDESQWWNERIAQCRNDATAASINEENDGQNRSDGYNLWNNYQNAHIFKDIPNVNVWLFLNSLSNFKSVEIEMLKKWLFCTPRTRYDWELLLKPILNGKVPVTVRDHDGFVLYKYGAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKJ4</accession>
    <name>C5DKJ4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Presequence translocated-associated motor subunit PAM17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0F05192g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the PAM complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Mitochondrion inner membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the PAM17 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554432.1">
        <property type="nucleotide sequence ID" value="XM_002554386.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKJ4"/>
    <dbReference type="FunCoup" id="C5DKJ4">
        <property type="interactions" value="67"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKJ4"/>
    <dbReference type="GeneID" id="8292630"/>
    <dbReference type="KEGG" id="lth:KLTH0F05192g"/>
    <dbReference type="eggNOG" id="ENOG502S1B1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068297_2_0_1"/>
    <dbReference type="InParanoid" id="C5DKJ4"/>
    <dbReference type="OMA" id="MIFGFDP"/>
    <dbReference type="OrthoDB" id="5970083at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001405">
        <property type="term" value="C:PAM complex, Tim23 associated import motor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013875">
        <property type="entry name" value="Pam17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28021">
        <property type="entry name" value="PRESEQUENCE TRANSLOCATED-ASSOCIATED MOTOR SUBUNIT PAM17, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28021:SF1">
        <property type="entry name" value="PRESEQUENCE TRANSLOCATED-ASSOCIATED MOTOR SUBUNIT PAM17, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08566">
        <property type="entry name" value="Pam17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 12" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="6 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="9 12" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="102"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU367146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR23995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="195" mass="22061" checksum="9FD226902B412A62" modified="2009-07-28" version="1">MLSARVIAYTRPAAKLASRNLSTVQPRFSEQSKSTDAKNEAQTNGLFTWTDFFQLRKQERRINLGSSIVTAFLSTNASWAYLSTMQIDPMQTIMGFDPLVVVSAALMASGAFGYLLGPIFGTTVFKMKQKTNLANYNKKTKDFLRHVITNRVDSSSQSFSNPVPDYYGEKIGSVAEYRQWLRDCHAFRRKAKEFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKR2</accession>
    <name>C5DKR2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">NADH-cytochrome b5 reductase</fullName>
            <ecNumber evidence="12">1.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0F06798g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>2 Fe(III)-[cytochrome b5] + NADH = 2 Fe(II)-[cytochrome b5] + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46680"/>
            <dbReference type="Rhea" id="RHEA-COMP:10438"/>
            <dbReference type="Rhea" id="RHEA-COMP:10439"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11 12">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.6.2.2" evidence="12"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554500.1">
        <property type="nucleotide sequence ID" value="XM_002554454.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKR2"/>
    <dbReference type="FunCoup" id="C5DKR2">
        <property type="interactions" value="118"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKR2"/>
    <dbReference type="GeneID" id="8292701"/>
    <dbReference type="KEGG" id="lth:KLTH0F06798g"/>
    <dbReference type="eggNOG" id="KOG0534">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003827_9_0_1"/>
    <dbReference type="InParanoid" id="C5DKR2"/>
    <dbReference type="OMA" id="CLDPENW"/>
    <dbReference type="OrthoDB" id="432685at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090524">
        <property type="term" value="F:cytochrome-b5 reductase activity, acting on NADH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06183">
        <property type="entry name" value="cyt_b5_reduct_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000069">
        <property type="entry name" value="NADH-cytochrome b5 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.80:FF:000009">
        <property type="entry name" value="NADH-cytochrome b5 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001834">
        <property type="entry name" value="CBR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008333">
        <property type="entry name" value="Cbr1-like_FAD-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19370">
        <property type="entry name" value="NADH-CYTOCHROME B5 REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19370:SF143">
        <property type="entry name" value="PLASMA MEMBRANE-ASSOCIATED COENZYME Q6 REDUCTASE PGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00970">
        <property type="entry name" value="FAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00406">
        <property type="entry name" value="CYTB5RDTASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11 12" id="KW-0274">FAD</keyword>
    <keyword evidence="4 11 12" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 12" id="KW-0520">NAD</keyword>
    <keyword evidence="8 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="40"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="14">
        <location>
            <begin position="66"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601834-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR24063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="308" mass="35043" checksum="8AF30934CFC3A2F2" modified="2009-07-28" version="1">MSDKTKLLDEPIHGIYIPIGLLFVGLAIACFTIKDYTFLWILPFAGLLIAARFTIAYRRKRSVFPNKWSSLELEDQTLISKNTAMYRFKMRTPLEALNFPVGHHLAVRVPVDGKELVRYYTPVSPRYQPGHFDIIVKSYADGQVSKYFAGLKPGATVDFKGPVGRFNYVTNSYKHIGMIAGGSGITPMLQILNEIITTPEDFTNVSLIYANETENDILLKDELDEIAEKYPNFEVNYVLNKPSSNWRGDVGYVTKEHMEKYLPPFSSDSRLVMSGRPEMIRMLLDYAEELGWPRGIEKSKGDDQVFAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKS0</accession>
    <name>C5DKS0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0F06996p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0F06996g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554508.1">
        <property type="nucleotide sequence ID" value="XM_002554462.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKS0"/>
    <dbReference type="FunCoup" id="C5DKS0">
        <property type="interactions" value="1781"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKS0"/>
    <dbReference type="GeneID" id="8292710"/>
    <dbReference type="KEGG" id="lth:KLTH0F06996g"/>
    <dbReference type="eggNOG" id="KOG0102">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005965_2_1_1"/>
    <dbReference type="InParanoid" id="C5DKS0"/>
    <dbReference type="OMA" id="MGTDWKI"/>
    <dbReference type="OrthoDB" id="2401965at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11734">
        <property type="entry name" value="ASKHA_NBD_HSP70_Ssc1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000014">
        <property type="entry name" value="Chaperone protein DnaK HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.30:FF:000003">
        <property type="entry name" value="Heat shock protein 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.10:FF:000007">
        <property type="entry name" value="Heat shock protein, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000004">
        <property type="entry name" value="Molecular chaperone DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000003">
        <property type="entry name" value="Molecular chaperone DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00332">
        <property type="entry name" value="DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012725">
        <property type="entry name" value="Chaperone_DnaK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001413">
        <property type="entry name" value="PRK00290.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02350">
        <property type="entry name" value="prok_dnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="557"/>
            <end position="591"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR24071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="647" mass="69818" checksum="5FE1AF8743F6F6F7" modified="2009-07-28" version="1">MLSAKSVLRNSANPLRSFVRMNSNKVQGQVIGIDLGTTNSAVALMEGKVPKIIENAEGARTTPSVVAFTKDGERLVGIPAKRQAVVNPENTLFATKRLIGRRFEDIEVQRDIKQVPYKIVKHSNGDAWLEARGQTYSPAQIGGFVLNKMKETAEAYLGKNVKNAVVTVPAYFNDSQRQATKDAGQIVGLNVLRVVNEPTAAALAYGLEKSDSKVVAVFDLGGGTFDISILDIDNGVFEVKSTNGDTHLGGEDFDIFLLREIVANFKKESGINLENDRMAIQRIREAAEKAKIELSSTVSTEVNLPFITADASGPKHINMKFTRAQFESLTEPLVKRTVDPVKKALKDASLATSDVSDVILVGGMSRMPKVVETVKSLFGKEPSKAVNPDEAVAIGAAIQGAVLAGEVTDVLLLDVTPLSLGIETLGGVFTRLIPRNTTIPSKKSQIFSTAAAGQTSVEIRVFQGERELVRDNKLIGNFTLSGIPPAPKGVPQIEVSFDIDADGIINVSARDKASNKDASITVAGSSGLSDSEIEQMVSDAEKYREQDEARKSAIESANKADQLANDTESSLKEFEAKVDKAEAQKVKDQITSLREIVARVQSGEEVNADDLKTKTEELQTSSMKLFEQMYKNDSSNNASSEGGEPKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>C5DL29</accession>
    <name>C5DL29_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">[RNA-polymerase]-subunit kinase</fullName>
            <ecNumber evidence="3">2.7.11.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">KLTH0F09504g</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.23" evidence="3"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554617.1">
        <property type="nucleotide sequence ID" value="XM_002554571.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DL29"/>
    <dbReference type="FunCoup" id="C5DL29">
        <property type="interactions" value="1166"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DL29"/>
    <dbReference type="GeneID" id="8292824"/>
    <dbReference type="KEGG" id="lth:KLTH0F09504g"/>
    <dbReference type="eggNOG" id="KOG0659">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_181_1_1"/>
    <dbReference type="InParanoid" id="C5DL29"/>
    <dbReference type="OMA" id="CLESEYF"/>
    <dbReference type="OrthoDB" id="1732493at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070985">
        <property type="term" value="C:transcription factor TFIIK complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008353">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07841">
        <property type="entry name" value="STKc_CDK7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000554">
        <property type="entry name" value="CMGC/CDK/CDK7 protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000097">
        <property type="entry name" value="Putative cyclin-dependent kinase 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037770">
        <property type="entry name" value="CDK7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF0">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 16" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="7"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="129"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="13"/>
            <end position="21"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14 15">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR637770-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR637770-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAR24180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="308" mass="35250" checksum="4639CE955688AB08" modified="2009-07-28" version="1">MISQAEYSKEKKVGEGTYAVVYVGTKQSSNRKVAIKEIKTSGFKDGLDMSAIREVKYLQEMQHINVIELIDVYMAQSNLNLVLEFLPADLEMIIKDTAILFTQADIKSWLLMTLRGVHHCHRNFILHRDLKPNNLLLAPDGQLKLADFGLARNMGSPQDFLTSNVVTRWYRAPELLFGARHYTGAIDMWSVGVIFAELMLRIPYLPGKDDIDQIDVTFRALGTPTDKDWPEISTFGTYNKIQFYPPPSREELRRRFIAATENALDLMSGMLIMNPHKRWDPIQCLTNQYFVELPEPTTPADLPQLNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>C5DL41</accession>
    <name>C5DL41_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N(6)-L-threonylcarbamoyladenine synthase</fullName>
            <ecNumber evidence="1">2.3.1.234</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">QRI7</name>
        <name evidence="9" type="ordered locus">KLTH0F09768g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in mitochondrial tRNAs that read codons beginning with adenine. Probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. Involved in mitochondrial genome maintenance.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>L-threonylcarbamoyladenylate + adenosine(37) in tRNA = N(6)-L-threonylcarbamoyladenosine(37) in tRNA + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:37059"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10163"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74418"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.3.1.234"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="7">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the KAE1 / TsaD family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.234" evidence="1"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554629.1">
        <property type="nucleotide sequence ID" value="XM_002554583.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DL41"/>
    <dbReference type="FunCoup" id="C5DL41">
        <property type="interactions" value="433"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DL41"/>
    <dbReference type="GeneID" id="8292836"/>
    <dbReference type="KEGG" id="lth:KLTH0F09768g"/>
    <dbReference type="eggNOG" id="KOG2707">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023208_4_1_1"/>
    <dbReference type="InParanoid" id="C5DL41"/>
    <dbReference type="OMA" id="NAAMIGC"/>
    <dbReference type="OrthoDB" id="10259622at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061711">
        <property type="term" value="F:tRNA N(6)-L-threonylcarbamoyladenine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072670">
        <property type="term" value="P:mitochondrial tRNA threonylcarbamoyladenosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01445">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000905">
        <property type="entry name" value="Gcp-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017861">
        <property type="entry name" value="KAE1/TsaD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017860">
        <property type="entry name" value="Peptidase_M22_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022450">
        <property type="entry name" value="TsaD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00329">
        <property type="entry name" value="gcp_kae1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735">
        <property type="entry name" value="TRNA N6-ADENOSINE THREONYLCARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735:SF6">
        <property type="entry name" value="TRNA N6-ADENOSINE THREONYLCARBAMOYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00814">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00789">
        <property type="entry name" value="OSIALOPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01016">
        <property type="entry name" value="GLYCOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Gcp-like" evidence="8">
        <location>
            <begin position="64"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR24192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="391" mass="43533" checksum="7BEB5B7B0EEA38D9" modified="2009-07-28" version="1">MFRSRTIRWLTSLGGRVSKVCSRRNYRVLAIETSCDDTCVAVIDRKSRQEPPVLLAHLKETLDSEKHGGIVPTKAHLHHQIKVGSLVQQALRQTSSERSIDLVCVTRGPGMPGSLSGGLDFAKGLAVAWDKPLVGVHHMLGHLLISRMHTNGSSPRYPFVSLLVSGGHTIFVHSKSISEHEILCNTIDIAIGDSLDKCAREIGIKGNMIAKNMEKFINENLEEAQSQTVDMSLPNPLRNQNGRMNVQAFSFAPFLTAVRTAMAKNKMEKCTKETRSMAYQIQEALFEHLITKLKLVISLRPDIFECTRHLVCSGGVGANRRLQQKLRDELSFQFQEFHYPEPSLCTDNAVMIGWAGIEIFETLGIKTELGVTPVKKWPLSDLLNVPGWIST</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2010-10-05" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>C5DL76</accession>
    <name>AIM3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName>Altered inheritance of mitochondria protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">AIM3</name>
        <name type="ordered locus">KLTH0F10648g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane raft</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <text evidence="1">Localizes within detergent-insoluble glycolipid-enriched membranes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the AIM3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554664.1">
        <property type="nucleotide sequence ID" value="XM_002554618.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DL76"/>
    <dbReference type="FunCoup" id="C5DL76">
        <property type="interactions" value="55"/>
    </dbReference>
    <dbReference type="GeneID" id="8292876"/>
    <dbReference type="KEGG" id="lth:KLTH0F10648g"/>
    <dbReference type="eggNOG" id="ENOG502S02E">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_324433_0_0_1"/>
    <dbReference type="InParanoid" id="C5DL76"/>
    <dbReference type="OMA" id="DNPFRRY"/>
    <dbReference type="OrthoDB" id="3973404at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051016">
        <property type="term" value="P:barbed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031370">
        <property type="entry name" value="Aim3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17096">
        <property type="entry name" value="AIM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000399602" description="Altered inheritance of mitochondria protein 3">
        <location>
            <begin position="1"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="39"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="338"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="393"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="799"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="52"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="104"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="406"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="422"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="525"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="638"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="654"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="718"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="731"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="753"/>
            <end position="766"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="842" mass="89574" checksum="9A7238E3FC032A9A" modified="2009-07-28" version="1">MSDFWDRNKGSIMSGLATAGKQGYKGTKFVAKTGYKAGKQHYNSSKNKREGNSNNSSSSDLTISSPPPLTADVSNFPPPPVKPGQNQYHGSSRGGSGNVPPAATSRTSSTLPSSYSSEQNPIIPQIGNPQAVPYQVPAPVPQYTTADSQAPHIASRAATIVPGTQLPISSHPNNTAPATYNTRPVPLPHRAPDNNAAAGLVADPQLLSNTSLPRATRQLLTIDTASIATQNPNISQEAFAVPPANTMASPFPQTTHASYLAASQSPVQPESIASNKVSEPTPAVPAVPTRTYVRAPAALPVQPLPQEERQAMPQAMPQVMPQAMPQVMPQAMPQVMPQAMAQTSQPFPEENGVSDVQELKSPTSATTVKPYSWKDSEELKESLRIKIQNVDISSLPTPPVHRNRSESPSSETSSLRSKIKSRSQSPSGLKSPTQSTAVNLKTTETGKAMSTSRQTPQQTGRSSREGEGANNGIAGRYDSSVQVNFPPPPKPSRSETQTPANRDMRSSSSPPVKPTTAPRAPPRLPSRSVPSQPPVTSSVPKLSSTANGKADRENIKPKSAVSGAYNYDIKVDFQPPPKPFRKDKESSQQAPRSVPSFEKPSKYGPPPASNAETAQGRSIRAPPQIPSRAASLDPPPAYDSSHSHAPSAPPASNVELQSLSASSIQAGAEKTVKVAGSTIQIPKTKKAPPIKKALPGSLQALDTHKSNPEASAHLTEKGKKRPPKPAKKQSLMEQPNKTASNGGNSRGNFDQELEARLKSRSFHEKPAAVPSLVEHSNSFISSGKIKAAAPPVKPKIPIRLASNEALQPNEQAEVKDDSDGDNPFRRYLKNAVPSENDRLHKN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2010-11-30" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLA7</accession>
    <name>ASA1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName>ASTRA-associated protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">ASA1</name>
        <name type="ordered locus">KLTH0F11440g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the ASTRA complex involved in chromatin remodeling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the ASTRA chromatin remodeling machinery complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat ASA1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554695.1">
        <property type="nucleotide sequence ID" value="XM_002554649.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLA7"/>
    <dbReference type="FunCoup" id="C5DLA7">
        <property type="interactions" value="65"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLA7"/>
    <dbReference type="GeneID" id="8292908"/>
    <dbReference type="KEGG" id="lth:KLTH0F11440g"/>
    <dbReference type="eggNOG" id="ENOG502QU4T">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045414_1_0_1"/>
    <dbReference type="InParanoid" id="C5DLA7"/>
    <dbReference type="OMA" id="LVCCNTQ"/>
    <dbReference type="OrthoDB" id="7668193at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19854:SF1">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN SUBUNIT BETA-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19854">
        <property type="entry name" value="TRANSDUCIN BETA-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0156">Chromatin regulator</keyword>
    <keyword id="KW-0539">Nucleus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0853">WD repeat</keyword>
    <feature type="chain" id="PRO_0000402212" description="ASTRA-associated protein 1">
        <location>
            <begin position="1"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="repeat" description="WD 1">
        <location>
            <begin position="13"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="repeat" description="WD 2">
        <location>
            <begin position="58"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="repeat" description="WD 3">
        <location>
            <begin position="111"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="repeat" description="WD 4">
        <location>
            <begin position="234"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="416" mass="47017" checksum="643317DD75ABE2CB" modified="2009-07-28" version="1">MQGHQPAAYTLRKHETEVTCLKVVSWEPYPVLASGDAKGELYLWNLVTRRPFAGYKLETKAQIIYIKWLNNYLIVLSKDHTLRFLQLHNETAVAIRGWAGNQFELQSFKIVYEVPVNTLNFANVVVTHMRESQYRLWCCNTMDSESIDVYEFDVKEQRSLRRLVSGINLYKAIIKAGNWSTGESLDKTGIVMCFLEHNGVVYLGYECGFVLGLQLVERSGENPTIVVSYVSSVHYPEPVLSLCYNEREEVILSSSTNDAIGVHALQAHSVNVSESEIAFFSIEEGIAIKRDLTLINKSIHLPTSKIGHLGCVDGRLIAVDWSGRTLVCEKDETKFCFSKQRSNVLVDESSAGTFDQGKAPRCHIKATSMACIGKQTLRELRGIRRGEKRRTELCANRSWCFTGYEDGSVIMQSFET</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLE4</accession>
    <name>C5DLE4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0F12364p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0F12364g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554732.1">
        <property type="nucleotide sequence ID" value="XM_002554686.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLE4"/>
    <dbReference type="FunCoup" id="C5DLE4">
        <property type="interactions" value="157"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLE4"/>
    <dbReference type="GeneID" id="8292950"/>
    <dbReference type="KEGG" id="lth:KLTH0F12364g"/>
    <dbReference type="eggNOG" id="ENOG502QPU6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003477_3_0_1"/>
    <dbReference type="InParanoid" id="C5DLE4"/>
    <dbReference type="OMA" id="WEKCKIM"/>
    <dbReference type="OrthoDB" id="10249045at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005740">
        <property type="term" value="C:mitochondrial envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000964">
        <property type="term" value="P:mitochondrial RNA 5'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013943">
        <property type="entry name" value="Pet127"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31014">
        <property type="entry name" value="MITOCHONDRIAL TRANSLATION SYSTEM COMPONENT PET127-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31014:SF0">
        <property type="entry name" value="MITOCHONDRIAL TRANSLATION SYSTEM COMPONENT PET127-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08634">
        <property type="entry name" value="Pet127"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="763" mass="87293" checksum="006547C3E7FE9B8F" modified="2009-07-28" version="1">MLSPSRCLTLHRCRFNTSVSLANLKGFSTDSGGEVGKELEHALNLSNEISKTSEQKLHSTQAGEDQEATRTKASGKASLKYVKEVDRMLNRERGPTRSQGQRILETANKILQRKEGSAKTFKAQFCVQPSGISFEPIKATGGADAKPPKLCHELDRVLFQPMNFHNLQDARSGTYNFSKWLENIIKVDDFDFEAVSEFVTASKDKKMLELTQDHNKRYYSSTSSMTGILSHMHFLISNFRKTNMSMISKHFAERSAGFTWGAQLPAVVIMKRMKQEKEVYSIDSDKSSDRELILSLLGHALETVLTTEEKQFKQVYDKSSRTDASVRTPPAGSYHYAKIQDFVLRSQLDAYHDKLPGTGVFDLKTRAVAAVRHDIAYVEKNDNYTGYQIQQTLGKFESFERELFELIRSTMLKYSLQARIGCMDGIFVAYHNISKMFGFQYLPFEEMDHILHSYKCRSFENVLEGRSEAFKAIFGEEDFIVKHDYANFERKISSAIADSEFKISMSVLNNLLRLVESELPDKSYSCRLVFKAEKKRVFDKSGNSRKVPVLNVIVAPLTEEQTNEFQNTDLRKECLQDNGKTNNYIQRMRQLNEKTAKTLIGFEVFVEHEVQHHPDTIKQPRFADKKSTVLDEKAKRFVKAATQKNYYEDLAEWKHVQFFHPLDILKWKPKFEVNQITNEDKLRKLYLAYLDDRLSALRGQSTSDKPPKASRDIISERIKNFMSGESASNGERGATADKTEVSQLQAVLRAYSKKGELAVKHSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLR2</accession>
    <name>C5DLR2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0G02794p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0G02794g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555160.1">
        <property type="nucleotide sequence ID" value="XM_002555114.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLR2"/>
    <dbReference type="FunCoup" id="C5DLR2">
        <property type="interactions" value="127"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLR2"/>
    <dbReference type="GeneID" id="8293423"/>
    <dbReference type="KEGG" id="lth:KLTH0G02794g"/>
    <dbReference type="eggNOG" id="ENOG502QRN9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092544_0_0_1"/>
    <dbReference type="InParanoid" id="C5DLR2"/>
    <dbReference type="OMA" id="IMKTGNY"/>
    <dbReference type="OrthoDB" id="9973266at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004422">
        <property type="term" value="F:hypoxanthine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032263">
        <property type="term" value="P:GMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046100">
        <property type="term" value="P:hypoxanthine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032264">
        <property type="term" value="P:IMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032265">
        <property type="term" value="P:XMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000033">
        <property type="entry name" value="Xanthine phosphoribosyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43363">
        <property type="entry name" value="HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43363:SF1">
        <property type="entry name" value="HYPOXANTHINE-GUANINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="3">
        <location>
            <begin position="13"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR24723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="214" mass="24453" checksum="B690E5B320E26038" modified="2009-07-28" version="1">MSLEKQYISYNNVHQLCQEAAVKIKNFKPDLIIAIGGGGFIPARILRTFLKEENVPTVRIFAIILSLYEDLASESSMEEKPGVTVNRTQWIDYEQCKLDLVGKNVLIVDEVDDTRTTLHYALNELEKDAAEQAKAKGIDLEQQPEMKTNFGIFVLHDKVKAKKADLPADLLNDENRYFAARKVPDTWYAYPWESKDIVYHTKMAIEQGNDVFLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLS3</accession>
    <name>C5DLS3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">IMP-specific 5'-nucleotidase 1</fullName>
            <ecNumber evidence="12">3.1.3.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0G03036g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">IMP-specific 5'-nucleotidase involved in IMP (inositol monophosphate) degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>IMP + H2O = inosine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:27718"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17596"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="EC" id="3.1.3.99"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3 12">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the ISN1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.-" evidence="12"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555171.1">
        <property type="nucleotide sequence ID" value="XM_002555125.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLS3"/>
    <dbReference type="FunCoup" id="C5DLS3">
        <property type="interactions" value="107"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLS3"/>
    <dbReference type="GeneID" id="8293434"/>
    <dbReference type="KEGG" id="lth:KLTH0G03036g"/>
    <dbReference type="eggNOG" id="ENOG502QR24">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031816_1_0_1"/>
    <dbReference type="InParanoid" id="C5DLS3"/>
    <dbReference type="OMA" id="WGVLACQ"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008253">
        <property type="term" value="F:5'-nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006190">
        <property type="term" value="P:inosine salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071590">
        <property type="term" value="P:nicotinamide riboside biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071592">
        <property type="term" value="P:nicotinic acid riboside biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009453">
        <property type="entry name" value="ISN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28213">
        <property type="entry name" value="IMP-SPECIFIC 5'-NUCLEOTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28213:SF1">
        <property type="entry name" value="IMP-SPECIFIC 5'-NUCLEOTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06437">
        <property type="entry name" value="ISN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028836">
        <property type="entry name" value="ISN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 12" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR028836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR24734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="432" mass="49166" checksum="CBD3C2B3A99A33FA" modified="2009-07-28" version="1">MPSRYRVEYQLKSHRKDELIEWIKGLLAVPFVLHSASHDPAARQILRQRYAEVFVAVEALIDEQVSVGKLARQSEDREFLVGRTRLAQLVPSIGPFFTRLPLRQAFLHEDHKRAISARTMVSPSFNDIRHILNSAQILQMRECHDVRLVTFDGDVTLYEDGGSLTATSKVVPFLLSLLRSGICVGIVTAAGYDEAQKYMERLFGLMTSLKDDTSISADFKRNLVVLGGESNYLFRYEPSQHELVSIENKAWIKPPMCHWSESDMTETLDLAESLLRDFRTTLNLPEESTIIRKPRAVGIVPGNRWNPETKRTIRVKMDREQLEEMVLTLQHRLEDFAPARRIQFSCFDGGSDVWCDIGGKDLGVAALQHYFDPYNPIKPCQTMHIGDQFAPMGSANDFKARLSGCTLWISSPQETVEVLRCLTAGFDHYFQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLT2</accession>
    <name>C5DLT2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 12">Protein-S-isoprenylcysteine O-methyltransferase</fullName>
            <ecNumber evidence="3 12">2.1.1.100</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0G03234g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>[protein]-C-terminal S-[(2E,6E)-farnesyl]-L-cysteine + S-adenosyl-L-methionine = [protein]-C-terminal S-[(2E,6E)-farnesyl]-L-cysteine methyl ester + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:21672"/>
            <dbReference type="Rhea" id="RHEA-COMP:12125"/>
            <dbReference type="Rhea" id="RHEA-COMP:12126"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90510"/>
            <dbReference type="ChEBI" id="CHEBI:90511"/>
            <dbReference type="EC" id="2.1.1.100"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Endoplasmic reticulum membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the class VI-like SAM-binding methyltransferase superfamily. Isoprenylcysteine carboxyl methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.100" evidence="3 12"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555180.1">
        <property type="nucleotide sequence ID" value="XM_002555134.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLT2"/>
    <dbReference type="FunCoup" id="C5DLT2">
        <property type="interactions" value="521"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLT2"/>
    <dbReference type="GeneID" id="8293443"/>
    <dbReference type="KEGG" id="lth:KLTH0G03234g"/>
    <dbReference type="eggNOG" id="KOG2628">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065200_0_2_1"/>
    <dbReference type="InParanoid" id="C5DLT2"/>
    <dbReference type="OMA" id="IKREEAY"/>
    <dbReference type="OrthoDB" id="422086at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004671">
        <property type="term" value="F:protein C-terminal S-isoprenylcysteine carboxyl O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007323">
        <property type="term" value="P:peptide pheromone maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019236">
        <property type="term" value="P:response to pheromone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1630:FF:000018">
        <property type="entry name" value="Protein-S-isoprenylcysteine O-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007269">
        <property type="entry name" value="ICMT_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025770">
        <property type="entry name" value="PPMT_MeTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12714">
        <property type="entry name" value="PROTEIN-S ISOPRENYLCYSTEINE O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12714:SF9">
        <property type="entry name" value="PROTEIN-S-ISOPRENYLCYSTEINE O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04140">
        <property type="entry name" value="ICMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51564">
        <property type="entry name" value="SAM_ICMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 12" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-0589">Pheromone response</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="113"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="140"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="202"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR24743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="264" mass="30211" checksum="B864E99C6E2077F8" modified="2009-07-28" version="1">MIEEDSGEPKSRRRSLPQDYDGDDVGDVGVIIKGQVYPYIEKNPLDSISLTGFLLGGTVGLSIGLLPHSFFKNFNIYLVAVSVFHFLEFYTTAKYNPGKVTSDSFLFNNGKAYTFSFVFAVSEALLESIFFDWKTRFSSWYHTTAAIIGLMLMLLGQYIRTSAMITAGRSFSHQVKTSQNPDHVLITTGIYAQLRHPSYFGYFWWALGTQLWLMNPVSFALFAYVLWKFFHSRINYEERYLNSFFGEEYTKYTQNVGVGIPFIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLX1</accession>
    <name>C5DLX1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0G04202p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0G04202g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF11 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555219.1">
        <property type="nucleotide sequence ID" value="XM_002555173.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLX1"/>
    <dbReference type="FunCoup" id="C5DLX1">
        <property type="interactions" value="264"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLX1"/>
    <dbReference type="GeneID" id="8293485"/>
    <dbReference type="KEGG" id="lth:KLTH0G04202g"/>
    <dbReference type="eggNOG" id="KOG3219">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048787_0_0_1"/>
    <dbReference type="InParanoid" id="C5DLX1"/>
    <dbReference type="OMA" id="GNGWMFR"/>
    <dbReference type="OrthoDB" id="28335at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08048">
        <property type="entry name" value="HFD_TAF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045127">
        <property type="entry name" value="TAF11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006809">
        <property type="entry name" value="TAFII28_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13218:SF8">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13218">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04719">
        <property type="entry name" value="TAFII28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TAFII28-like protein" evidence="7">
        <location>
            <begin position="144"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR24782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="355" mass="40593" checksum="CD74BA3EBF491AFD" modified="2009-07-28" version="1">MVEPQGPLDTVPEENYPPLLTQNNYWNTKQLINQVISEDQEYVTWKLKDVRTGGTMNAYLNDFMKNEYDQEDGSSAEDIADAHTAEAPLQRVRKRNGGPTGRVSKASPINSVPKNVQFPRDIYDSQLAALPQPRELDQSEQLNLLLTNLDEEQMNRFEVFRRTSLAKNNIKKISSIITNQTVAANINLLLAGVGKIFIGEIVEKAQEVKKRKLAALMASRFRDKQITAYRLKKILKKLTLMVEGPDASYDDSVDENESDLCEDDEDENLVNEVNTYNRLIRSKINSEASRLGIIRHYNKLVKSFNALDVSVEKFTNCPLLPEHIREAWRLYRAENDTVPQATWRTQGEGNGWMFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLY9</accession>
    <name>C5DLY9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transcriptional adapter 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0G04598g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555237.1">
        <property type="nucleotide sequence ID" value="XM_002555191.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLY9"/>
    <dbReference type="FunCoup" id="C5DLY9">
        <property type="interactions" value="738"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLY9"/>
    <dbReference type="GeneID" id="8293503"/>
    <dbReference type="KEGG" id="lth:KLTH0G04598g"/>
    <dbReference type="eggNOG" id="KOG0457">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018273_3_0_1"/>
    <dbReference type="InParanoid" id="C5DLY9"/>
    <dbReference type="OMA" id="YNGNHRP"/>
    <dbReference type="OrthoDB" id="270417at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070461">
        <property type="term" value="C:SAGA-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02335">
        <property type="entry name" value="ZZ_ADA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000087">
        <property type="entry name" value="Transcriptional adapter 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000115">
        <property type="entry name" value="Transcriptional adapter 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.90:FF:000008">
        <property type="entry name" value="Transcriptional adapter 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041983">
        <property type="entry name" value="ADA2-like_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016827">
        <property type="entry name" value="Ada2/TADA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007526">
        <property type="entry name" value="SWIRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055141">
        <property type="entry name" value="TADA2A_B-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000433">
        <property type="entry name" value="Znf_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043145">
        <property type="entry name" value="Znf_ZZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12374:SF20">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTER 2-ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12374">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTOR 2 ADA2 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04433">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22941">
        <property type="entry name" value="TADA2A-like_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00569">
        <property type="entry name" value="ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF025024">
        <property type="entry name" value="Transcriptional_adaptor_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00291">
        <property type="entry name" value="ZnF_ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50934">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01357">
        <property type="entry name" value="ZF_ZZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50135">
        <property type="entry name" value="ZF_ZZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZZ-type" evidence="11">
        <location>
            <begin position="2"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="13">
        <location>
            <begin position="60"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="10">
        <location>
            <begin position="65"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="SWIRM" evidence="12">
        <location>
            <begin position="350"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR025024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR24800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="435" mass="50405" checksum="DD9490737B689DAC" modified="2009-07-28" version="1">MSNKFHCDVCSSDCTNRVRISCAECPEYDLCVPCFSQGLYNGNHRPFHNYKIIETNSYPILCEDWGADEELALIKGSQTLGLGNWQDVADHIGNRTKEEVDEHYTKYYLNSPYYPIPDITQNIDIPQEQFLNDRRRRIERFRGQPLQPPRKPIASVPSCHEVQGFMPGRLDFETEFENEAEGPVKDMVFEPDDQPLDIEVKLIILDIYNSRLTTRAEKKRLLFENGLMEYRKLQGIDKKRSKESKDLFNTLKAYARIMTPRDFEEFSKDVLEELRCRSRIHQLQEWRSNGITTLEAGLKYERDKQARIMTLERFGSSLHSSSNGNGNGRHRVSSAHRANADYGQNYNEAASRKKALTTGDIQHGADFGLLSGEEQQLCIQLKILPKPYLAIKEVMFRELLRAGGNMKKKTCRELLNIDPAKANRIYDFFQSQRWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DM94</accession>
    <name>C5DM94_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0G06996p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0G06996g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555342.1">
        <property type="nucleotide sequence ID" value="XM_002555296.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DM94"/>
    <dbReference type="FunCoup" id="C5DM94">
        <property type="interactions" value="171"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DM94"/>
    <dbReference type="GeneID" id="8293611"/>
    <dbReference type="KEGG" id="lth:KLTH0G06996g"/>
    <dbReference type="eggNOG" id="KOG3033">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048756_0_0_1"/>
    <dbReference type="InParanoid" id="C5DM94"/>
    <dbReference type="OMA" id="AHWTNLS"/>
    <dbReference type="OrthoDB" id="75169at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.10">
        <property type="entry name" value="Diaminopimelate Epimerase, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003719">
        <property type="entry name" value="Phenazine_PhzF-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00654">
        <property type="entry name" value="PhzF_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13774:SF32">
        <property type="entry name" value="ANTISENSE-ENHANCING SEQUENCE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13774">
        <property type="entry name" value="PHENAZINE BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02567">
        <property type="entry name" value="PhzC-PhzF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016184">
        <property type="entry name" value="PhzC_PhzF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54506">
        <property type="entry name" value="Diaminopimelate epimerase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="1">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR016184-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="290" mass="31960" checksum="E0EDA460A6518B7E" modified="2009-07-28" version="1">MTITVPFKQVDVFTSKLFKGNPVAVVNFLDVEESSITQDTLQSIARWTNLSETTFLFKATDPKSDYKLRIFTPMCELPFAGHPTVGSCQAYLEFSGRDAVGNIYQQCGVGVVELTVEDKKISLKGPPPKVMEVPSAETSDYEGSLSAEFFQKPMLLDVGPKWIVGLMRDPQSCYELEPDFSKMKASTIKYGAIGLVVAGKYPGEDKYEMRAFAPGDGVLEDPVCGSGSLALAAYLQQLNNYESNYEFEIYQGRRLYRDGKVSAKIEHLGGEVEYFIGGEAITIVNGEISV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2023-06-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMA3</accession>
    <name>C5DMA3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0G07194p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0G07194g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555351.1">
        <property type="nucleotide sequence ID" value="XM_002555305.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMA3"/>
    <dbReference type="GeneID" id="8293620"/>
    <dbReference type="KEGG" id="lth:KLTH0G07194g"/>
    <dbReference type="HOGENOM" id="CLU_1540328_0_0_1"/>
    <dbReference type="InParanoid" id="C5DMA3"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR24914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="174" mass="18787" checksum="BDCBC418A93DB939" modified="2009-07-28" version="1">MPARNRQNVCKVALVATPEMCVCVRVCEAILAAQSELRTRMRRATKALCNHVTCAVSMPRTMQRTHAALAFAEHLRPLSALPPLMQVTVVGVCVCRVAVLPMCGAPDGATDEGSDRNCSITILPKLSYLGLGSCLDSCLGSCLGSCLSFVFYVSLRLQVIGNIPNFLQCQNQEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMH5</accession>
    <name>C5DMH5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Acetyltransferase component of pyruvate dehydrogenase complex</fullName>
            <ecNumber evidence="7">2.3.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0G08998g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>N(6)-[(R)-dihydrolipoyl]-L-lysyl-[protein] + acetyl-CoA = N(6)-[(R)-S(8)-acetyldihydrolipoyl]-L-lysyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:17017"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="Rhea" id="RHEA-COMP:10478"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="ChEBI" id="CHEBI:83111"/>
            <dbReference type="EC" id="2.3.1.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
        <text evidence="7">Binds 1 lipoyl cofactor covalently.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Eukaryotic pyruvate dehydrogenase (PDH) complexes are organized as a core consisting of the oligomeric dihydrolipoamide acetyl-transferase (E2), around which are arranged multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide dehydrogenase (E3) and protein X (E3BP) bound by non-covalent bonds.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the 2-oxoacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.12" evidence="7"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555423.1">
        <property type="nucleotide sequence ID" value="XM_002555377.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMH5"/>
    <dbReference type="FunCoup" id="C5DMH5">
        <property type="interactions" value="1195"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMH5"/>
    <dbReference type="GeneID" id="8293700"/>
    <dbReference type="KEGG" id="lth:KLTH0G08998g"/>
    <dbReference type="eggNOG" id="KOG0557">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016733_10_2_1"/>
    <dbReference type="InParanoid" id="C5DMH5"/>
    <dbReference type="OMA" id="TMEFESF"/>
    <dbReference type="OrthoDB" id="537444at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045254">
        <property type="term" value="C:pyruvate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004742">
        <property type="term" value="F:dihydrolipoyllysine-residue acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006086">
        <property type="term" value="P:pyruvate decarboxylation to acetyl-CoA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06849">
        <property type="entry name" value="lipoyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000010">
        <property type="entry name" value="Acetyltransferase component of pyruvate dehydrogenase complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000003">
        <property type="entry name" value="Acetyltransferase component of pyruvate dehydrogenase complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.320.10">
        <property type="entry name" value="E3-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001078">
        <property type="entry name" value="2-oxoacid_DH_actylTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045257">
        <property type="entry name" value="E2/Pdx1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036625">
        <property type="entry name" value="E3-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006257">
        <property type="entry name" value="LAT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004167">
        <property type="entry name" value="PSBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01349">
        <property type="entry name" value="PDHac_trf_mito"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23151">
        <property type="entry name" value="DIHYDROLIPOAMIDE ACETYL/SUCCINYL-TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23151:SF90">
        <property type="entry name" value="DIHYDROLIPOYLLYSINE-RESIDUE ACETYLTRANSFERASE COMPONENT OF PYRUVATE DEHYDROGENASE COMPLEX, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00198">
        <property type="entry name" value="2-oxoacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02817">
        <property type="entry name" value="E3_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47005">
        <property type="entry name" value="Peripheral subunit-binding domain of 2-oxo acid dehydrogenase complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51826">
        <property type="entry name" value="PSBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3 7" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="9">
        <location>
            <begin position="33"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="Peripheral subunit-binding (PSBD)" evidence="10">
        <location>
            <begin position="173"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="126"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="126"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="155"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00065810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR24986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="471" mass="50030" checksum="714F30A24CBA5E8D" modified="2009-07-28" version="1">MSSFIRTVPRIRSAVLRSARLQLRTYASSYPPHTVIGMPALSPTMTQGNIAVWNKQVGDKLEPGEAIAEIETDKAQMDFEFQEDGFLAKILEPAGAKDLPVGKPIAVYVEEEGDVAAFKDFKVEETAPAKSEKPVADAKPAEDNKSAKDDKPAKKPSASKQAASGKPAGDRIFASPLAKNIALQHGVALKSVTGTGPHGRITKSDVEEFLSKSPAASAGGSAAAGTAAAAAAPAGPGAQYEDIPISNMRKIIGDRLLESTSGTPAYIVSSQISVSKLLKLRQSLNASGKDQYKLSVNDMLIKAVTVAAQRVPDANAYWLANEGVIRKFKNVDVSVAVATPNGLLTPIVKNAHAKGLVSISSEIKELGKRAKINKLKPEEFQGGTICISNLGMNNAVSMFTSIINPPQSTILAIGTVQRVPVEDAGAEYGFIFEDKINITGTFDHRTIDGARAGDFMRELKKVVENPLEMLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMK7</accession>
    <name>C5DMK7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0G09724p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0G09724g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555455.1">
        <property type="nucleotide sequence ID" value="XM_002555409.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMK7"/>
    <dbReference type="FunCoup" id="C5DMK7">
        <property type="interactions" value="179"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMK7"/>
    <dbReference type="GeneID" id="8293733"/>
    <dbReference type="KEGG" id="lth:KLTH0G09724g"/>
    <dbReference type="eggNOG" id="KOG0504">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006901_0_0_1"/>
    <dbReference type="InParanoid" id="C5DMK7"/>
    <dbReference type="OMA" id="PLHFSGE"/>
    <dbReference type="OrthoDB" id="194358at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0085020">
        <property type="term" value="P:protein K6-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171:SF8">
        <property type="entry name" value="BRCA1-ASSOCIATED RING DOMAIN PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="343"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="376"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="411"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="581"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="287"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="480"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="649"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="799"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="31"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="62"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="120"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="133"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="147"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="207"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="220"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="253"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="649"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="678"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="703"/>
            <end position="712"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR25018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1073" mass="119374" checksum="9781919B9F07D6CB" modified="2009-07-28" version="1">MSDGSRSSSNEPGLPTVKKRSLSNYLSNIQNRREELGRVVKKEEDKAKHGAATSGANSTKEAGVKEDAPKHSDSLGVKKTENEMRDELREKSMLEASRDLGNTDSSAGQEKVSKGAPKPPGDENGGKEENKNGSGSDQSLSTTRAPSESKDNCTVKNKSEDPKSETVGEASEAEKDSRKNANEDSSDVKTAPKGSIIKSELIQLIEEPEKEKPREKNEEIESELSEVDSDAPTEPASPPKPRLGRLIRGDQLKSPTKSNSRYFSTGNSEESELSDIEDLNHLPISSSILHGDSSPHKPSHIGLNSSPVNLVHSKPKPTTAVKSHVAISKTTKTRKSGVHRDAGGRTRLQIACDKGKHDVAKRLIQEGYDVNDQDNAGNSPLHEAALNGHLDIVKLLLSNGADVNIQSHEPVKDTPLIDAAANGHLKVVKLLLKYNADPTISNAKGLTAFESIDEDSDFDSEERKLVRDLKYELRKAATEFGNNPGRQARSSSKNGFSESERSSSNARMEEEFFWTDISSKAGREKLLRASREGKLSYVGAYLENGGRVDFKSFTEAVRSGHEDITSLFLAFGAQPNLVLRDGQTPLMMSLGRGHIGTAKLLLEAGANPLAKDKKGKSVLSYAKNSELGLVSEDEVQLIENAIRKERRSNEEFWNRESPGSERTEKSQQQEEQDESTKASNQSPQKARLLSTKSTVPQKRPHSYESQSNTNISDVKEPSPEVDAGSRNPFIQDSFEGYAGNRRSPRDGSLSPGTVLPMSDPPRSQTSTPAYPGSVKAVETTEEREQRLKAEESYRQKRLLNKKKKEQEFLQKIAEDERKREEEIARQDAERAKKLEEEKAKQAEREIQKRTEAELERRRKIRAQYPLGLRAIDFDRKDEFQRFLPIYYVTIDGLRHVLDLQVCILLKDESFLAKCENGRDIPQAQKEQIWNIFKFIFLYGGRKSTDKAFGSQALSGIDRLSFKDLAELDNAERQKFANLPLRWIPWNSVSFPEGTEIIKEAAESQMIEVSLEQHTTSWLGAQTNSTNASHYNSHQVPLSTAIRQIPQEVYGDLPPRLRNRSSILKVLKGPRPSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMP9</accession>
    <name>C5DMP9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Histone H3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0G10670g</name>
        <name evidence="14" type="ordered locus">KLTH0G16258g</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
            <strain evidence="13">CBS 6340</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2009-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Genoscope - CEA"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CBS 6340</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="10">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the histone H3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555497.1">
        <property type="nucleotide sequence ID" value="XM_002555451.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMP9"/>
    <dbReference type="SMR" id="C5DMP9"/>
    <dbReference type="FunCoup" id="C5DMP9">
        <property type="interactions" value="1071"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMP9"/>
    <dbReference type="GeneID" id="8293775"/>
    <dbReference type="KEGG" id="lth:KLTH0G10670g"/>
    <dbReference type="KEGG" id="lth:KLTH0G16258g"/>
    <dbReference type="eggNOG" id="KOG1745">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078295_4_0_1"/>
    <dbReference type="InParanoid" id="C5DMP9"/>
    <dbReference type="OMA" id="HIFAEMA"/>
    <dbReference type="OrthoDB" id="4030662at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22911">
        <property type="entry name" value="HFD_H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000010">
        <property type="entry name" value="Histone H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000164">
        <property type="entry name" value="Histone_H3/CENP-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11426">
        <property type="entry name" value="HISTONE H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00622">
        <property type="entry name" value="HISTONEH3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00428">
        <property type="entry name" value="H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00322">
        <property type="entry name" value="HISTONE_H3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00959">
        <property type="entry name" value="HISTONE_H3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="9 10" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="12">
        <location>
            <begin position="44"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR25060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR25304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="136" mass="15356" checksum="A6115FEB480AC67A" modified="2009-07-28" version="1">MARTKQTARKSTGGKAPRKQLASKAARKSAPSTGGVKKPHRYKPGTVALREIRRFQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAIGALQESVEAYLVSLFEDTNLAAIHAKRVTIQKKDIKLARRLRGERS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMR1</accession>
    <name>C5DMR1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0G10956p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0G10956g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555509.1">
        <property type="nucleotide sequence ID" value="XM_002555463.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMR1"/>
    <dbReference type="FunCoup" id="C5DMR1">
        <property type="interactions" value="88"/>
    </dbReference>
    <dbReference type="GeneID" id="8293788"/>
    <dbReference type="KEGG" id="lth:KLTH0G10956g"/>
    <dbReference type="eggNOG" id="ENOG502SA5C">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_150136_0_0_1"/>
    <dbReference type="InParanoid" id="C5DMR1"/>
    <dbReference type="OMA" id="MDIFAYF"/>
    <dbReference type="OrthoDB" id="4035964at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR25072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="143" mass="16629" checksum="42D6F260EBE9B932" modified="2009-07-28" version="1">MDFYAFLVLFVIIVAFVLLLPLLSGTFAYKFRKPAARAGKTDHEHLSRTQKLKEKLEFSKDENPIKFQLRDNASTSSRNKPFEVDSKTGLKKRTIGKYSADPNEFDYDLTELIEEDVLEEKKENELRYQQFAGREQEESEALV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMS2</accession>
    <name>C5DMS2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Acetyl-coenzyme A synthetase</fullName>
            <ecNumber evidence="6">6.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0G11198g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>acetate + ATP + CoA = acetyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23176"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.1" evidence="6"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555520.1">
        <property type="nucleotide sequence ID" value="XM_002555474.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMS2"/>
    <dbReference type="FunCoup" id="C5DMS2">
        <property type="interactions" value="795"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMS2"/>
    <dbReference type="GeneID" id="8293799"/>
    <dbReference type="KEGG" id="lth:KLTH0G11198g"/>
    <dbReference type="eggNOG" id="KOG1175">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_3_6_1"/>
    <dbReference type="InParanoid" id="C5DMS2"/>
    <dbReference type="OMA" id="TVHTKKI"/>
    <dbReference type="OrthoDB" id="1706066at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003987">
        <property type="term" value="F:acetate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019427">
        <property type="term" value="P:acetyl-CoA biosynthetic process from acetate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05966">
        <property type="entry name" value="ACS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000001">
        <property type="entry name" value="Acetyl-coenzyme A synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011904">
        <property type="entry name" value="Ac_CoA_lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032387">
        <property type="entry name" value="ACAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02188">
        <property type="entry name" value="Ac_CoA_lig_AcsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001208">
        <property type="entry name" value="PRK00174.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095:SF245">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16177">
        <property type="entry name" value="ACAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 6" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acetyl-coenzyme A synthetase N-terminal" evidence="9">
        <location>
            <begin position="39"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="7">
        <location>
            <begin position="97"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="8">
        <location>
            <begin position="546"/>
            <end position="636"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR25083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="682" mass="74662" checksum="857566AC00B3A3FF" modified="2009-07-28" version="1">MTKQHKVVHEAHNVELREAPEHFYKSQPAPGHVTSMDQYREMYEQSIQDPEGFFGPMAREHLHWDRPFTKVCAGSLENGDASWFLNGELNASYNCVDRHAFANPSKPALIYEADDEKDNRVVTFGELLRQVSEVAGVLKSWGVKKGDTVAVYLPMIAEAVVAMLAVARLGAIHSVVFAGFSAGSLKERVVDAGSRVVITCDEGRRGGKTVHTKKIVDEGLAGVDSVSHILVFQRTGSAGIPMKPGRDFWWHEETAKHRGYLPPTPVNAEDPLFLLYTSGSTGSPKGVVHSTAGYLLGAALTTKYVFDIHPQDVLFTAGDVGWITGHTYALYGPLALGTATIIFESTPAYPDYGRYWRIIERHKATHFYVAPTALRLIKRVGEAEISKYDTSSLRVLGSVGEPISPDLWEWYHDKVGNKNCVICDTMWQTESGSHLIAPLAGAVGTKPGSATVPFFGVDACLIDPVSGEELKGNDVEGVLAVKSPWPSMARSVWNNHSRYIETYLKPYPGFYFTGDGAGRDHDGYYWIRGRVDDVVNVSGHRLSTAEIEAALGEHDGVSESAVVGITDELTGQAVIAFVSLKDGYLQENASEGDMAHITPDNLRRELVLQVRGEIGPFAAPKSVVVVNDLPKTRSGKIMRRVLRKVASNEADQLGDMSTLANPDCVPSIISAVENQFLIQKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMU4</accession>
    <name>C5DMU4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0G11682p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0G11682g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555542.1">
        <property type="nucleotide sequence ID" value="XM_002555496.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMU4"/>
    <dbReference type="FunCoup" id="C5DMU4">
        <property type="interactions" value="210"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMU4"/>
    <dbReference type="GeneID" id="8293821"/>
    <dbReference type="KEGG" id="lth:KLTH0G11682g"/>
    <dbReference type="eggNOG" id="ENOG502RZ32">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069841_1_0_1"/>
    <dbReference type="InParanoid" id="C5DMU4"/>
    <dbReference type="OMA" id="PFYLTHM"/>
    <dbReference type="OrthoDB" id="19092at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000283">
        <property type="entry name" value="NAP1-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="66"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="122"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="153"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="40"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="128"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="163"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR25105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="187" mass="21257" checksum="63D616025BBB9AF2" modified="2009-07-28" version="1">MDHHQECSSADEVAEAGYISIKDFAYDKSNPLHFGYFGESSDDEEGSEECESDSRRQSIVLPSEYIVNRRAVALYDFVPENDNELELREGDVLFIGYRHGQGWLVAENAERTRTGLVPEEYVSLTEPGDGDDDDSESGEERPRPFYLTHMIAQSMNNSRGGQNDEEWEDIDDLESHLNEHLRISNSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMW2</accession>
    <name>C5DMW2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0G12144p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0G12144g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555560.1">
        <property type="nucleotide sequence ID" value="XM_002555514.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DMW2">
        <property type="interactions" value="227"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMW2"/>
    <dbReference type="GeneID" id="8293842"/>
    <dbReference type="KEGG" id="lth:KLTH0G12144g"/>
    <dbReference type="eggNOG" id="ENOG502QRR7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_225471_0_0_1"/>
    <dbReference type="InParanoid" id="C5DMW2"/>
    <dbReference type="OMA" id="EQPNEQH"/>
    <dbReference type="OrthoDB" id="2149224at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032065">
        <property type="term" value="P:maintenance of protein location in cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13365">
        <property type="entry name" value="PH_PLC_plant-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053005">
        <property type="entry name" value="Nuclear_Pos-Cytoskel_Interact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024774">
        <property type="entry name" value="PH_dom-Mcp5-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28190">
        <property type="entry name" value="NUCLEAR MIGRATION PROTEIN NUM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28190:SF1">
        <property type="entry name" value="NUCLEAR MIGRATION PROTEIN NUM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12814">
        <property type="entry name" value="Mcp5_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="3413"/>
            <end position="3525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="313"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2684"/>
            <end position="2705"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2858"/>
            <end position="2883"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2916"/>
            <end position="2948"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2993"/>
            <end position="3035"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="3314"/>
            <end position="3338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="3556"/>
            <end position="3582"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="108"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="328"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="353"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="3556"/>
            <end position="3570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="3571"/>
            <end position="3582"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR25123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="3582" mass="401916" checksum="A19AED30494A58E9" modified="2009-07-28" version="1">MGLPKEEREKPPEADLKTQLEDIQRRIDHETEEALSKRASKRRAPFSNILSQRDKRTSMPVFAGSSTHNNVANSTSSKDVNFVVGLSENLLLECRRLQADNESKASSLRSLQQDYQALKATFEKLSRRLHGTEKESAVLKDSNWELETKLQQMSQQLKLLRDASEKSQKELRAQLDTYNVTRSELEELSLDRLGLKKELESVHQNYLKEIADLKKNVSDLNEENDELQQKLDSMNNQILQLRKDMSNSTSPTLSSAGANAEPVTLAKNITKVSENVRDLAFDSSSQSSVADLESANQVIETLRKQISKLKSARSHTFTNKQGAHDQESSTLSSSPLPLTGTLTSNKHMTPTKSIKRHKSKNHERRHSIQSSDDFNFSISSECIRAQTGTDDEGLESDVSDTLDIVRNPILDELDTSKYEIDPEEFRRYAEKNNLIVLHKSEFDVINGPHKNSGDRNQNDIIKELKNQGYKVFQQAEFETLCDSLISQKPSHCELESLAASADKKLISHDEYTNLKNPPLPWLLDQVTAAGYAAIKANEYQVIQNRAENPNLEILKAGLQRHGLMPVKEELFQKMVHPDVNSISVYAKTMGYELVDTKSWASIKEIAHNPTLDFLSDAAKGRGQVVKPVEEYEALSHPSLEAVSNLASEYGYVTFPKEDFDKIKNFDIQWAKEQASSHNFVLVSKQEYQKLQHPSMEELKTKSLALDNILVPKEVHKELSEPSISKLKELAAEKDFAVISSTELRQLEKCVESPDEGQIRLGAKKLKLTVLHDSQYLSMQDELKNPKFDVIQKCIESSTMDGFTDWVGREFDSVLIKKDEHEKLINLSRNPTLEHLSSKASDRGYCVVEEKELSELKRRLACDDLDILSSKCLEHGKTLVDKEFYDKLYEEHENPSIDYLRSNAKLVNYELIESGKLSSIRMQITSPPFIFLEEKIKEMGYVPIKKDDYSILKRTVELPDRKYLEDKAGQLGLVLIAKRDHETMKKTFEAPDASFLEEKAGQLDFILVPKKDHENLKKTVEAPNVSFLEEKAGQLDFVLVPKNDHATMKKTFEAPDASFLEEKAGQLDFILVPKKDHETLKKTVEAPDVSFLEEKAGQLDFVLVPKKDHETLKKTVETPDASFLEEKAGELGFVLVPKTDHESMKKDNAAMKKAVETPDASYLEEKAGQLDFILVPKKDHETLKKTVETPDVSFLEEKAGQLDFDLVPKKDHATMKKIVEMPDASFLEKKAGELDFVLVPKKDHETLKKAVETPDASYLAAKAGLLGCIIIPKNDHRAMKKTVETPDFSYLEAKAKLLGCVVISKKDHETMKTDNAAMKKTVETPDVSFLEEKAGQLDFVLVPKKDHAAMKKTVKTPDASYLEEKAGQLDFVLVPKKDHETMKKDHAAMKKTVETPDASYLEEKAGQLDFVLVPKKDHETMKKDHAAMKKAVETPDASYLEEKAGQLDFVLVPKKDHETLKKTVETPDASYLEEKAGQLDFVLVPKKDHETMKKDHAAMKKTVETPDASYLEEKAGQLDFVLVPKKDHETMKKDHAAMKKTVETPDVSFLEEKAGQLDFALVPKKDHETLKNTIEMPDASYLEEKAGQLDFILVPKKDHETLKKTVETPDVSFLEEKAGQLDFDLVPKKDHAAMKKTVKTPDASYLEEKAGQLDFVLVPKKDHETLKNLVESPSVSYLREKSEGLDFILIAATELQKLQESLMKPSLSYLKEKAEALMFTLTSQEDYNKMVEKIETPDIQYLESKALGHKLIPVQEYERLKSFEGTTAIDLCRSKCEDAGFAVTERKDYDTLLQKATEPSVQELNTMATERNRILLTIDEFEKMKKDHDSPPKNYLLSHLETYNLCAISQKDYKSLISAASDDAALGKVIELGYKVFTLEELAKLENEIIDNAKFSDIEQRLRNEAMAIISIADLKELKRPLLEKIDIDTISSYCEQNGLLVLTTVEHDNLKNATTNLTFDELQDLAASSGSKLVPLTDYNELLEKAISPTLSFLEQEARKIGMVLVSKASYDREQKELHHPSLEILINHANEIDKVVVDKNIYETLKALSESPSTEFLKEKCQEMNYCFLPNSEYLLLSETIQKPSQDYLSSKAAALDKVLVDISELEDMKRRDCSPSVDDLKAKASTMGMNLLSEADYEVFETIRSPSIQYLLSKSDELGYAMIKNEDFEALQSVRERMSIDELNSIAAKHAYTVVSATELKKLQSELDSPSEEFLSTKSETVGKVLVEKSDYDTDRETLDLAKNPTADFVSALGSKIDHVVLPSTQYRKMENDLENPPIGPLTSYLEGQGLKLVSQKDYSDLVSLTQSPDISYLESSAKQFERILVPVSNYSDLLKTRDDPELDFLLEKIKKHPEYVALEKNKFDLLEKNAVNPPLELLKVNATSHGKVLVDVEAWECLEHLNENPTDEFLEKHALKNNKVLIDKKKFEDLKEESLNPSFDVLERNIKASNQVLLSEEDYEKLKNSSEAPPIDLLEKKLESLDLISLTKVQYDRLKTNSEYSSLEKVEQVAQRTGNTVVELPQRNVLNSNDSTHPQSEDHDFLILTHENRVPKLSSLMDLSENQLSEFCQKFSLLPVTKTEYDLISTELLPSDLKQFVTSSLSNSETASKQSSTDQRNGILFGTLQKIMKSIDFCIVDSANDDFGAHKGSSILVQGARRDSRDPFSGRLTEQGCSRSLIKIESQSPKSASGHIMASQDEGKHSDSLPVKGVVLLKPEDFDLLLSSSDNGVVNMALNGDSVTFESETGETMRVIASFLPNSGLTFSVEELKEAALKSGYQLVPHGALGSSQEEMDARQRRESLLSCSSQASLPSVANSRSSYRSLNYTSTNESEYYDAQQSFAESISFDNSSATFSVGPSEYEDARDQTLSEDGEPLSRTSTVRGGHLSQGFSLSQVSNFAKDLGYILVKEDDDTVHGRSSGMSKPIGSLAAPSLSEGSGNEARSNQEDFESISNYSLSDDMLRAQAARFGMTIITCEELAEYESLKKNMAESPKLPATSSSVLRSTPEAENNEGPDYQNQEIVERETTDDDTDFDFSKERPNLQYLIDAASILGFKLVQKESVPSVSEKVSAVVEKDESGFQNPVEGCPRRVSVTDYYPSKEELIERAHEYGLVALNAEHFAQIKEELAGSARKLTLDDIMIKSAEFGLVPIQRAQFEQIKQELSNPTLTKDQVIASAIDFGLVAVDKEELKRLSHFSDSRATDIEDDDLTSSGIGGSEVTDNSEDRREIYNLAKKLGLMCIPENKFVVTTTASVIDTNNVVVLPSSYYENLLAKEQEALKMTTNDELQAEAKRRGLHMGVKSSAHASSMASPFGQAKISRQNTIKSTGSSDSNSRRSLAEAAAAAAYNDYEMFSTRTREHSRSASTLKPTALGMDVDIPHIRHTSFDGGISLATVASLSEPSIIPALTQTVIGEYLHKYYRRLGAFSQISSRHERYFWVHPYTMTLYWSTNNPVLENPASNKTRAAAILGIESVEDSNPYPAGLYHKSILITTESRPIKITCATRQRHNIWFNSLRYLLQRNMDGINLDDTATDPNDANKIYQLPGETAKLTNQRLSSTRRLTSSGSANRPSSTRTLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>C5DN04</accession>
    <name>C5DN04_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0G13134p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0G13134g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555602.1">
        <property type="nucleotide sequence ID" value="XM_002555556.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DN04"/>
    <dbReference type="GeneID" id="8293885"/>
    <dbReference type="KEGG" id="lth:KLTH0G13134g"/>
    <dbReference type="eggNOG" id="ENOG502SCNR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136303_0_0_1"/>
    <dbReference type="InParanoid" id="C5DN04"/>
    <dbReference type="OMA" id="FKCLFQL"/>
    <dbReference type="OrthoDB" id="4032406at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR25165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="157" mass="18518" checksum="A425CEF892F663D1" modified="2009-07-28" version="1">MGKDLALKELEFLEHFLRINRSQQPVFNSFVLQKEQLRQCNIQLWSFRTLDKFTALYQLHDVLQDTKVSDLTLYALLEKLNLLFAKGPDFEESMVMDSKLLTIALIEVLIRICRIISCDSTDSKVRHSLRKSILLSIHVQFTREYALKLWEQIEDQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>C5DN42</accession>
    <name>C5DN42_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0G13992p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0G13992g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TDE1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555640.1">
        <property type="nucleotide sequence ID" value="XM_002555594.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DN42"/>
    <dbReference type="FunCoup" id="C5DN42">
        <property type="interactions" value="546"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DN42"/>
    <dbReference type="GeneID" id="8293924"/>
    <dbReference type="KEGG" id="lth:KLTH0G13992g"/>
    <dbReference type="eggNOG" id="KOG2592">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029574_2_0_1"/>
    <dbReference type="InParanoid" id="C5DN42"/>
    <dbReference type="OMA" id="DKHCNPL"/>
    <dbReference type="OrthoDB" id="5963193at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005016">
        <property type="entry name" value="TDE1/TMS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10383">
        <property type="entry name" value="SERINE INCORPORATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10383:SF9">
        <property type="entry name" value="SERINE INCORPORATOR, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03348">
        <property type="entry name" value="Serinc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="83"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="127"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="196"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="225"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="258"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="298"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="399"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="437"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR25203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="470" mass="51764" checksum="6BFF302229ACF59D" modified="2009-07-28" version="1">MGAIISLPLTSAASFAASFFGSLSSSILVSSFKSLSGGSSSLATRLIYAALLLFNSLFSWISMSANYSFPWFKKSCTSTGECGFFTVHRLNFALGLLHLMLSSALVNVRSTTEKRAALQNSWWSLKLLAYGALVGLSFALPNKFFVFFSKWISVPSGSLFILVGLVLLVDFAHEWAEKCIQHVELEDENSSKWQKFLVAGTSIMYVGAMAMTTVMYVLFCHNNCTMNQVAATVNLLLSIITTGLSIHPKVQEYNPRCGLAQSSMVAIYGTYLTMSALASEPDDRQCNPFVRSSKTRKFSAVLGSLFTFVAIAYTTTRAAANSAFNSNEQSIFLDGGDEVGYEGVSQSRNQLRSEAIRQAVEEGALPESALYDTAWAETSEGNGVSGDDERIATKYNYSLFHVIFFLATQWIAILLTINVTKDDVGDFIPVGRTYFYSWVKIVSAWICYGLYGWTLVAPMVLPERFGYEEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DN94</accession>
    <name>C5DN94_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0G15158p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0G15158g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the prokaryotic/mitochondrial release factor family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555692.1">
        <property type="nucleotide sequence ID" value="XM_002555646.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DN94"/>
    <dbReference type="FunCoup" id="C5DN94">
        <property type="interactions" value="61"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DN94"/>
    <dbReference type="GeneID" id="8293977"/>
    <dbReference type="KEGG" id="lth:KLTH0G15158g"/>
    <dbReference type="eggNOG" id="KOG2726">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089470_4_0_1"/>
    <dbReference type="InParanoid" id="C5DN94"/>
    <dbReference type="OMA" id="FMHGGRG"/>
    <dbReference type="OrthoDB" id="277888at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003747">
        <property type="term" value="F:translation release factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000065">
        <property type="entry name" value="Peptidyl-tRNA hydrolase domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052405">
        <property type="entry name" value="Mito_Transl_Release_Factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000352">
        <property type="entry name" value="Pep_chain_release_fac_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045853">
        <property type="entry name" value="Pep_chain_release_fac_I_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46203:SF1">
        <property type="entry name" value="MITOCHONDRIAL TRANSLATION RELEASE FACTOR IN RESCUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46203">
        <property type="entry name" value="PROBABLE PEPTIDE CHAIN RELEASE FACTOR C12ORF65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00472">
        <property type="entry name" value="RF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75620">
        <property type="entry name" value="Release factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Prokaryotic-type class I peptide chain release factors" evidence="6">
        <location>
            <begin position="33"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="112"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="119"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="131"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR25255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="155" mass="17802" checksum="2211012C20BA945D" modified="2009-07-28" version="1">MKALLRCGFHTIAARSIKKNKLPPRPKLSTQMETELEEKFLHGGRGPGGQKINKCNSKVQLKHLPSGIVVECQETRSRDQNRKLAREKLALRIAQWQGGGGPVAREVALHEWERQGKRSKERKSKDKHVKHQEVRRSAEMQKLQEEEDLLRSLLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNA3</accession>
    <name>C5DNA3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Condensin complex subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0G15378g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the CND1 (condensin subunit 1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555701.1">
        <property type="nucleotide sequence ID" value="XM_002555655.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNA3"/>
    <dbReference type="FunCoup" id="C5DNA3">
        <property type="interactions" value="651"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNA3"/>
    <dbReference type="GeneID" id="8293987"/>
    <dbReference type="KEGG" id="lth:KLTH0G15378g"/>
    <dbReference type="eggNOG" id="KOG0414">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001867_1_0_1"/>
    <dbReference type="InParanoid" id="C5DNA3"/>
    <dbReference type="OMA" id="CPLEKLW"/>
    <dbReference type="OrthoDB" id="436262at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000779">
        <property type="term" value="C:condensed chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000796">
        <property type="term" value="C:condensin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010032">
        <property type="term" value="P:meiotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000272">
        <property type="entry name" value="Condensin complex subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026971">
        <property type="entry name" value="CND1/NCAPD3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032682">
        <property type="entry name" value="Cnd1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007673">
        <property type="entry name" value="Condensin_cplx_su1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024324">
        <property type="entry name" value="Condensin_cplx_su1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14222">
        <property type="entry name" value="CONDENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14222:SF2">
        <property type="entry name" value="CONDENSIN COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12717">
        <property type="entry name" value="Cnd1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12922">
        <property type="entry name" value="Cnd1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017127">
        <property type="entry name" value="Condensin_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5 10" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 10" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="6 10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Condensin complex subunit 1 N-terminal" evidence="14">
        <location>
            <begin position="68"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="Condensin complex subunit 1 C-terminal" evidence="13">
        <location>
            <begin position="964"/>
            <end position="1124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="477"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="852"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="434"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="481"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR017127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF12717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF12922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR25264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1148" mass="128727" checksum="89BDB73FD36BBDD6" modified="2009-07-28" version="1">MPDFSLSEQLTKFQTSDKYGYPAVENPSSELNTVTDALAVSPELIDDNEETLESLMDLAHGFSQLQPQFQTQLTYLVSSSFGNLAQAIDGSFSGGSTNAELMAMVPQWKRQLEEYGYITHVLLHFLQNDIAAAASQNTMAKAGARTNTASSASSTSFKRTSNQIEIILSCVLKVFNLNISRMFPTTPERDLFIGLFTRPLYVLIETEQVLKMPAIKLFIIKIIGTAVKNHGQSSSTQNAILTSLTYFVHLNNFSAELLQAINDDFDFPQLTEDILRDISNKEFNSKDVKGPKSIATFLVKLSELIPRIVLRQMTLIVKLLNNSSFTLRCAVVEACGNIVIEISSKREDLELCKQSVDVLLELLEERFADSNPYVRTKAIQACLKICDLPIKFKKHRSNLVSLAVRSLQDRSSLVRRNSVKLLTRLLLTHQFDALHGTQLDLSEWETRLKEAEELLARTVEESQSDSGNALDNIIEKSVREGDEDSSEESDEDQVSRLEREAEDVPTTNSNAIFKMKLTIQYYKDAVSFIKSIHEGIERSCQLLFSRNRNEVLEVMDFFVLTDAFNVKLSKLGVRKMLHLVWMKGSNDEGTSIVAHLISCYNQLFLTPPEGFNFREKAAYIAKNLIRLTENTSAADLASLERLLGLIYEANFIDQDAINVLWAIYSSCSGEKDAFDEKQVHGSIIVLGMLSLADHQIALKGLDALLKVGLGEPGKKDLILLKYTCVAIQRMVPRSEKSVPAGIPRESEAVLKLHARITEFVDTSDYYPACEEAIKAIFAISSSPDLVGTEIIREKTMMTFGRKEDESHANNVASRTGSLSQLLFIVGQVAINAIVYLEKCETEFKRRKIDAEAHKHRGASDQPGTEAAEEHQQELEMIGGTNEDDFSDAIAFIKENELLFGEKSLLAKFGPLVEEIVSNNTKFADKMLQRTAVLCLEKFMCVSSKYCENNLPLLITIMEKSPDPIIRSNAILGLGDMAVCFNNLVDENTDFLYRRLHDGDLMVQKTCLMTVTFLILAGQVKVKGQLGQMAKCLENPDQGISDMCKLFFTELATKDNAIYNGFIDIFSSLSNDEDLGRTSFKRILKFLLSFIEKERHQKQLSEKLLVRLQKSETQKQWDDVAFVLNSLPFKSEKANAMLTEGFKMVTARN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNA7</accession>
    <name>C5DNA7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0G15466p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0G15466g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555705.1">
        <property type="nucleotide sequence ID" value="XM_002555659.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNA7"/>
    <dbReference type="FunCoup" id="C5DNA7">
        <property type="interactions" value="246"/>
    </dbReference>
    <dbReference type="GeneID" id="8293991"/>
    <dbReference type="KEGG" id="lth:KLTH0G15466g"/>
    <dbReference type="eggNOG" id="KOG1994">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046724_0_0_1"/>
    <dbReference type="InParanoid" id="C5DNA7"/>
    <dbReference type="OMA" id="DYMNMVI"/>
    <dbReference type="OrthoDB" id="786951at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025239">
        <property type="entry name" value="DUF4187"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000467">
        <property type="entry name" value="G_patch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039249">
        <property type="entry name" value="GPATCH11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21032">
        <property type="entry name" value="G PATCH DOMAIN-CONTAINING PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21032:SF0">
        <property type="entry name" value="G PATCH DOMAIN-CONTAINING PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13821">
        <property type="entry name" value="DUF4187"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01585">
        <property type="entry name" value="G-patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01173">
        <property type="entry name" value="DUF4187"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00443">
        <property type="entry name" value="G_patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50174">
        <property type="entry name" value="G_PATCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G-patch" evidence="2">
        <location>
            <begin position="86"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="207"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="207"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="234"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR25268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="322" mass="35653" checksum="15466789BB024ADD" modified="2009-07-28" version="1">MKRELAESDNDEKPPLPPKRARPLSSAERQERQSDAESSEEPDYLTMALPDEVPEHEFPGPEGPKAPSSDFPGPKKDHAHQKAAALMPKGFAMMNKMGFKMGGALGKGSGTAITEPIAVARNTKRQGIKRTSSLISLDEPSLEDVTSYRKRVQDNGGRARKARVLHRMQKTAFELSGDIERFTVDSDPRDYNCLWREYVVELKRKLKPKTSEEAPHLHELSTSVSKKLEEEVEANSSDSSSPEATPSASASASPGPTIAKTTGSSEDDELAIFQELPLDQQITGINIHLRVELNYCFYCGVQFESEKDLFEHCPGSTEEDHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNB5</accession>
    <name>C5DNB5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0G15642p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0G15642g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TAF9 family. CENP-S/MHF1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555713.1">
        <property type="nucleotide sequence ID" value="XM_002555667.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNB5"/>
    <dbReference type="FunCoup" id="C5DNB5">
        <property type="interactions" value="73"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNB5"/>
    <dbReference type="GeneID" id="8293999"/>
    <dbReference type="KEGG" id="lth:KLTH0G15642g"/>
    <dbReference type="eggNOG" id="ENOG502S7WI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100369_3_0_1"/>
    <dbReference type="InParanoid" id="C5DNB5"/>
    <dbReference type="OMA" id="WTQIENV"/>
    <dbReference type="OrthoDB" id="1872155at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071821">
        <property type="term" value="C:FANCM-MHF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031297">
        <property type="term" value="P:replication fork processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22919">
        <property type="entry name" value="HFD_CENP-S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029003">
        <property type="entry name" value="CENP-S/Mhf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22980:SF0">
        <property type="entry name" value="CENTROMERE PROTEIN S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22980">
        <property type="entry name" value="CORTISTATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15630">
        <property type="entry name" value="CENP-S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR25276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="118" mass="13459" checksum="105CF6607DC9C8F1" modified="2009-07-28" version="1">MSEQNSTSDSHMSEEQKQQLIPQLKGKLWYCLEQLVKKELPSDISYSPKFINALVELCFTQLVDIGGDLEAFARHAGRETIVVEDLMLRLRNSSDLQQLLQQKLEENTAVGPARRADR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNJ8</accession>
    <name>C5DNJ8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Dolichyldiphosphatase</fullName>
            <ecNumber evidence="6">3.6.1.43</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0G17622g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Required for efficient N-glycosylation. Necessary for maintaining optimal levels of dolichol-linked oligosaccharides. Hydrolyzes dolichyl pyrophosphate at a very high rate and dolichyl monophosphate at a much lower rate. Does not act on phosphatidate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a di-trans,poly-cis-dolichyl diphosphate + H2O = a di-trans,poly-cis-dolichyl phosphate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14385"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19506"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57497"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="EC" id="3.6.1.43"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Endoplasmic reticulum membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the dolichyldiphosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.43" evidence="6"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555796.1">
        <property type="nucleotide sequence ID" value="XM_002555750.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNJ8"/>
    <dbReference type="FunCoup" id="C5DNJ8">
        <property type="interactions" value="391"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNJ8"/>
    <dbReference type="GeneID" id="8294089"/>
    <dbReference type="KEGG" id="lth:KLTH0G17622g"/>
    <dbReference type="eggNOG" id="KOG3146">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074922_1_2_1"/>
    <dbReference type="InParanoid" id="C5DNJ8"/>
    <dbReference type="OMA" id="LTVYQHE"/>
    <dbReference type="OrthoDB" id="302705at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047874">
        <property type="term" value="F:dolichyldiphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03382">
        <property type="entry name" value="PAP2_dolichyldiphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039667">
        <property type="entry name" value="Dolichyldiphosphatase_PAP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11247:SF1">
        <property type="entry name" value="DOLICHYLDIPHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11247">
        <property type="entry name" value="PALMITOYL-PROTEIN THIOESTERASE/DOLICHYLDIPHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="7">
        <location>
            <begin position="58"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR25359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="239" mass="27141" checksum="56F3DC0CF30729D8" modified="2009-07-28" version="1">MNVSAVTLDPILIPFDDTYILYDPNDVISYVLVYYSLLPIGILIFYFSWFISSRELEAVIIAGGQVVNEALNNIIKNIIKEPRPASIGSSFQKDTIRSAYGMPSAHSQFMGFFVMYWSLKVVLHWRGLSNARKLSSILAMGFTTVMVAVSRIYLGYHTAFQVCVGVALGGFFGSMYYMAVGIVRYMGLIDWILGWPFCKRLWVKDSFNTNSLSLHDEYEAWMHRYSQARGKSNLAKKDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNL5</accession>
    <name>C5DNL5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0G18106p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0G18106g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555813.1">
        <property type="nucleotide sequence ID" value="XM_002555767.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNL5"/>
    <dbReference type="FunCoup" id="C5DNL5">
        <property type="interactions" value="65"/>
    </dbReference>
    <dbReference type="GeneID" id="8294190"/>
    <dbReference type="KEGG" id="lth:KLTH0G18106g"/>
    <dbReference type="eggNOG" id="ENOG502QRJ1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074989_0_0_1"/>
    <dbReference type="InParanoid" id="C5DNL5"/>
    <dbReference type="OMA" id="WLRLNFS"/>
    <dbReference type="OrthoDB" id="5581259at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071786">
        <property type="term" value="P:endoplasmic reticulum tubular network organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061024">
        <property type="term" value="P:membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051645">
        <property type="entry name" value="PER33/POM33_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12703:SF3">
        <property type="entry name" value="ABR032WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12703">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="23"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="123"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="170"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="194"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR25376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="265" mass="31042" checksum="397E80B3131CA2E4" modified="2009-07-28" version="1">MAVQKIIRKKPKAKVDPLAKQKIIWTAGHGLTLACGAIYAVFYLFQCLTFYRYRSWKTLFLIARPPINKPRTWLKWLWRLFPQISYRLSVIGVFLSYGVTSYQNWNALNPSWYDLLSKENFQSILIACLWLFSRATIFKLIPFMLTSYLHLTVKENESEEKESSAQNTQLLHVIAYSELIVAGILFLDTISFKGASGFVLALYLAIYWLRLNFSPYAQVTLLRLVVKLDKKVPPKFESQWKQVKEFLYLRMDESKKKRAEIAKPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNP3</accession>
    <name>C5DNP3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DNA-directed RNA polymerase</fullName>
            <ecNumber evidence="10">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0G18744g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the phage and mitochondrial RNA polymerase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="10"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555841.1">
        <property type="nucleotide sequence ID" value="XM_002555795.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DNP3">
        <property type="interactions" value="304"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNP3"/>
    <dbReference type="GeneID" id="8294136"/>
    <dbReference type="KEGG" id="lth:KLTH0G18744g"/>
    <dbReference type="eggNOG" id="KOG1038">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003364_1_0_1"/>
    <dbReference type="InParanoid" id="C5DNP3"/>
    <dbReference type="OMA" id="KWFEVDM"/>
    <dbReference type="OrthoDB" id="276422at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034245">
        <property type="term" value="C:mitochondrial DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001018">
        <property type="term" value="F:mitochondrial promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006390">
        <property type="term" value="P:mitochondrial transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1320.10:FF:000005">
        <property type="entry name" value="DNA-directed RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000041">
        <property type="entry name" value="DNA-directed RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.280:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1320.10">
        <property type="entry name" value="DNA-directed RNA polymerase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024075">
        <property type="entry name" value="DNA-dir_RNA_pol_helix_hairp_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046950">
        <property type="entry name" value="DNA-dir_Rpol_C_phage-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002092">
        <property type="entry name" value="DNA-dir_Rpol_phage-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037159">
        <property type="entry name" value="RNA_POL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029262">
        <property type="entry name" value="RPOL_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10102">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10102:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00940">
        <property type="entry name" value="RNA_pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14700">
        <property type="entry name" value="RPOL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01311">
        <property type="entry name" value="RPOL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00900">
        <property type="entry name" value="RNA_POL_PHAGE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00489">
        <property type="entry name" value="RNA_POL_PHAGE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 10" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase N-terminal" evidence="11">
        <location>
            <begin position="352"/>
            <end position="672"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR25404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1289" mass="145974" checksum="8DB3C8E07781D20C" modified="2009-07-28" version="1">MLRFSRSCSRMLVSKRLTSQLRCVSQTAFLRKPSGNIDGLSGATGREDIGFLKSSSVSMSPHFGPFDHSHAVVSAAESRDIMNLWSLLEACLHSGYMERAFSILKSLYALKSHQAYFIDDYNKFLMKYAETLHDIKDLDKKLFHDIQETFPACEYNDKTLAILIHHALKLSSSAEAKLEHCKIYFKMAVSGQRSILSNVDVLTVPNYKSLVHDLKLVSLNELPAAVRPLIESGNIVTGEMLSSQQSSEINAKALSHGEVALDRNIEPLDKDAKELLAVDTIGMKVVRHTLLGLSFTEDQKKQITSFNFDTSSNLLNFDQEKESIDFFEMFRNLKSEEERRSFESALDEFNQDRQHQLENRATDAAKERWRHDFEEAKSRGDLSIQKKLNIKLWEWFNLMLPLVKEEVRRCAAVANKTAQDTSLSPQEKKQNKARQEYGPYLTLVDPEKMCVITILELLKLNSTGGIIEGMRTARAVISVGKAIEMEFRAEMLLKNESHVFKDVNKKSHEFKLMVQRARATFRSIKIEESKVLWPQEARAKIGSILISMLIHVAKVKVEGVDPVTNEKIKGEAPAFSHGYQYHNGSKLGVLKIHRSLIHQLNGERLVAAVQPQLLPMLVKPRAWNSWNSGGYYYTQSTLIRSKDSPEQVAYLKAVSDAGAIDNVYRGLNVLGETPWTVNRKMFNVMSQVWNSGKPFLDIPGIQEKLTLTPQPPRDADPSVHREWKLKNKELANEFSGNRSARCDANYKLEIARAFLGEKFYFPHNLDFRGRAYPLSPHFNHLGNDMSRGLMTFWHGKRLGSTGLRWLKVHLSNLFGFDKAPLEDRVRFAEDHIDDIRDSAEHPLDGKCWWKEADKPWQALATCMELNEALKMSNPDDFVSHQPVHQDGTCNGLQHYAALGGDIEGATQVNLVPSDRPQDVYSHVAKLVVARLEKAAANGDETAALLKDKIKRKVVKQTVMTNVYGVTYIGATHQIDKQLSTLFNDHKRSYELSKYLTKHVFASVRELFEGAHLIQDWLGECAKRISKSIRLDVDEKTLKNGNKPDFMSSVIWTTPLGLPIVQPYRENGKKQVATNLQTVFISDPFAINPINARRQKAGFPPNFIHSLDASHMLLSAAKCGEKGLDFAAVHDSYWTHACDVDTMNVVLREQFIKLHEVDLIERLKDEFDERYKNYLQILKIDKKSEVAMKVTDLKKKLSQKLGRPFTLADEIYMEKKRIQLLSSEDASEQAEGANMVTTVSLVAGAGDISNLEKTSDASCLVVLAPLTLPQIPPKGDFDVRELEKSKYFFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>C5E249</accession>
    <name>C5E249_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein transport protein SEC31</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Protein transport protein sec31</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ordered locus">KLTH0H02134g</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">COPII-coated vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat SEC31 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555972.1">
        <property type="nucleotide sequence ID" value="XM_002555926.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5E249">
        <property type="interactions" value="772"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E249"/>
    <dbReference type="GeneID" id="8294285"/>
    <dbReference type="KEGG" id="lth:KLTH0H02134g"/>
    <dbReference type="eggNOG" id="KOG0307">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003033_2_0_1"/>
    <dbReference type="InParanoid" id="C5E249"/>
    <dbReference type="OMA" id="AQWAFGG"/>
    <dbReference type="OrthoDB" id="542917at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007029">
        <property type="term" value="P:endoplasmic reticulum organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.980">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.400">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1600">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.940.10">
        <property type="entry name" value="Functional domain of the splicing factor Prp18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024298">
        <property type="entry name" value="Sec16_Sec23-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021614">
        <property type="entry name" value="Sec31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040251">
        <property type="entry name" value="SEC31-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009917">
        <property type="entry name" value="SRA1/Sec31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13923">
        <property type="entry name" value="SEC31-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13923:SF11">
        <property type="entry name" value="SECRETORY 31, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11549">
        <property type="entry name" value="Sec31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07304">
        <property type="entry name" value="SRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12931">
        <property type="entry name" value="TPR_Sec16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47938">
        <property type="entry name" value="Functional domain of the splicing factor Prp18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <keyword evidence="7 15" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="100"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="247"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="Sec16 Sec23-binding" evidence="18">
        <location>
            <begin position="508"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="domain" description="SRA1/Sec31" evidence="17">
        <location>
            <begin position="1179"/>
            <end position="1292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="764"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="923"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="977"/>
            <end position="1033"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1075"/>
            <end position="1129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1146"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="808"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="847"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="923"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="940"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1000"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1163"/>
            <end position="1178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF07304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF12931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAR30110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1299" mass="139008" checksum="0615732D4C1B61D9" modified="2009-07-28" version="1">MVKLAEYPRTATFAWSYDRSPLLATGTASGTIDADFSSESTLEIWSLLSPHEATPQASITADAKFNDLDWSHDNAIIAGALENGVVEFFAPEEPRSVAKLAQHTTPVKTVTFNARQHNVLCSGGSKGEIYIWDANKIETPGYAPFVPGTAMTPMDEIYSLAWNQNQAHVFASAGSSGYASIWDLKAKKEVIHLSYASPVTGQRNQLSIVEWHPNNSTRIATASGNDSDPSILIWDLRNANVPMQVLSQPHTKGVLSLDWCKQDETLLLSSGRDNVCVLWNPEEGQSLTQYPTRGNWCFKTKFAPQTPDLFASASFDNKIEVQTLQNVTCSLDTDATATKQRESETEFWNNVSVQDANEKPIVKKLQAPAWYGNKSQAAQWAFGGKLVQITSDKKGVKISKPAIPGAEKNVLLDKALETKDFNPIINKRLVQSIDATNEEDWNLLEKLSLDGKDAFLSEALAFDDEDTDETKEKDVKDDGGDFFSSLNEKFVPEGSFRLDASDKLNDLFKSLVRGDKKSAISSALEQDLLLESLLIALDSDDQTLKQKVKNAYFTKHGKTSSLARVLHCVSEGDADDLVKNIEVDQWKYAVKAIDSYTSDLSKKNELFIALGDRLSDAGNRQDALLLYLSAQSLDKVASIWLKEFVGLESKLESKKETVYEAHLECLTEFVERFTVFISLISDDQHQSLANEELIAKFLEFVNLTSSNGDFDLALKFLEILPGDNEDVVTEKQRVLIASNKLPSAATKSQKPRYAGGNVLSGTSVAGLGLTNDRTPGGVKMPPAQPMQTVNPSAPFAPSTPFNQQTPLRSRAASFAAPAPELLKSNPYAPASTQATTGSKYAPPPITSPSSAPRGSSVASSVPITHKNAGTYAPAVAQPSVAGAVPPNPYAPSAGAYGNFSGQPAYSQPLKPVANVIGASAPGANSMSPPLAPPASNAASGQTPHLNKNANDGWNDLPLNVKEKPTRAKAVSVAPVSVAPSSAAPGPQVNFGSIPPPPLSRVTSSANLQTPVPQRVPSKTYTPPNGSASPASVNPYAPPLASAAPAPVVPSNPYAPSAVSTGQPVPGNPYAPAAAMPSNPYASPPPNGAPQPHLGKSYGPGSGLNGLSAPPASQKAPIGPPPKHMKRRTHASNDIDSANAILESVQKRSEAPTPQVGGAPPTSIPKNQPLSEVTSSGTTPAPLAAEQGSSSGIPVEQQPIVDFLSSELGRVTPLIPQEYTKQLKDCNKRLRILFGHLERQDLLTQPTVDKLHSIVALMKERRYADAMQVHVDIATNHAQEAGNWLTGVKRLIGIAEVTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2B9</accession>
    <name>C5E2B9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small COPII coat GTPase SAR1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Small COPII coat GTPase sar1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="ordered locus">KLTH0H03696g</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">COPII-coated vesicle membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 21">Belongs to the small GTPase superfamily. SAR1 family.</text>
    </comment>
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        <property type="protein sequence ID" value="CAR30180.1"/>
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    <sequence length="188" mass="21248" checksum="47636AB2EC00040A" modified="2009-07-28" version="1">MWDLFGWFRDILSSLGLWNKHGKLLFLGLDNAGKTTLLHMLKNDRLATLQPTWHPTSEELAIGNIKFTTFDLGGHIQARRLWKDYFPEVNGIVFLVDAADPERLNEARVELDALFNIAELKDVPFVILGNKIDSPNAVSETELRSALGLLNTTGGARIEGQRPVEVFMCSVVMKNGYLEAFQWLSQYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2M1</accession>
    <name>C5E2M1_LACTC</name>
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        <recommendedName>
            <fullName evidence="2">SWR1-complex protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0H06072g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Component of the SWR1 complex which mediates the ATP-dependent exchange of histone H2A for the H2A variant HZT1 leading to transcriptional regulation of selected genes by chromatin remodeling. Involved in chromosome stability.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SWC5 family.</text>
    </comment>
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    <proteinExistence type="inferred from homology"/>
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        </source>
    </evidence>
    <sequence length="300" mass="34657" checksum="270DF01883DD1EB0" modified="2009-07-28" version="1">MPNYLSQKLLRSTAMSKDNEEVQDTKGVLFNDEEDYNEEEDLDFDPSKEEGAQPNESDNDADGDTGDDDKNYEETNPDYSNIESGQGGLVRTRRARELEDELARRQKYEHIKQEGISQEAKSVWDELQQESTKRLHKSTSIMSQTKDSLGEGLTEELILIDRKYEFAGEVMHEKKWVPKSSAEAQEFLQSTKFTKNPDPSTKTKSAVNDQGLKLRRPLKRPAILEQIIAGALKPKLTTLEKSKLDWATYVDKENLNEELILHNKDGYLAKQDFLNRVETFKDDKYREMRKIQLQQRISDT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2010-10-05" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2Q0</accession>
    <name>GEP7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName>Genetic interactor of prohibitin 7, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">GEP7</name>
        <name type="ordered locus">KLTH0H06754g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in respiratory growth and required for cell survival in the absence of prohibitins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the GEP7 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556173.1">
        <property type="nucleotide sequence ID" value="XM_002556127.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2Q0"/>
    <dbReference type="FunCoup" id="C5E2Q0">
        <property type="interactions" value="19"/>
    </dbReference>
    <dbReference type="GeneID" id="8294489"/>
    <dbReference type="KEGG" id="lth:KLTH0H06754g"/>
    <dbReference type="eggNOG" id="ENOG502S4Y0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094335_0_0_1"/>
    <dbReference type="InParanoid" id="C5E2Q0"/>
    <dbReference type="OMA" id="DAMIHRE"/>
    <dbReference type="OrthoDB" id="4069787at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0496">Mitochondrion</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0809">Transit peptide</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="transit peptide" description="Mitochondrion" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000399739" description="Genetic interactor of prohibitin 7, mitochondrial">
        <location>
            <begin position="22"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="236"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="264"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="277"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="302" mass="34726" checksum="7C0D5B275E9306D7" modified="2009-07-28" version="1" precursor="true">MLSQYLRKHHLLSRRHQFMRCASQSRSPPRSLLQRQAKRRGEAEAVAPSQLIVTSLKDIFSTFQPSGFTQEDDELEAVKQREDAMQRLENGELRELLLHKFGARRIPSTTETGNSVGDLRIPPRNINQAFHNLTTQERELIEVFQSLGTPSMNWRDVPLVSKQLQFYISFGSYGPREGITFLGSKPEDFIWSKTSRRLLPGQTVRKLPKDATTNTWTCIPSRKANFERMKKGLDPGTRIIAWLGILIVMIASVRDYKQRRDSEATVKVSEFTEQETSEPQAAQQDTAPISKTPKSWYQFWKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2R1</accession>
    <name>C5E2R1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0H07018p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0H07018g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556184.1">
        <property type="nucleotide sequence ID" value="XM_002556138.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2R1"/>
    <dbReference type="GeneID" id="8294501"/>
    <dbReference type="KEGG" id="lth:KLTH0H07018g"/>
    <dbReference type="HOGENOM" id="CLU_301289_0_0_1"/>
    <dbReference type="InParanoid" id="C5E2R1"/>
    <dbReference type="OMA" id="SHIAECA"/>
    <dbReference type="OrthoDB" id="4036114at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR30322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="992" mass="111883" checksum="FBE8CF34DB72EB34" modified="2009-07-28" version="1">MQHETLESGSPPISYLKDLKNGNEVLIVEGDIVHLYRTKDSSFDTSRVTELKNSALHLSFRLVNPKSDFFLVLAFDLSITVFDIPGFKKVAKKRLKINEQDVDPRKTLRFLDSDSEQLYFSLSEKQLVSINKSDLLSESPFAKFISVHDTPYRIENAFCLNPNKGFFCLAKRDLIQNVLAFEVIHANTFRHRNNHSRYDRLLQEYTAAMDCGSSGELGTLVEMITLEVPKTVVFLFSRGVIIFNNKVCVFENKYDENTRESLCLDGLIPGASLYQGEEGLRLSILSTKGETISTTIDLRKPKSLQWNSVTLNSFLKKPLDSMLCYKCLGHGQILLISKREGMVFADYQKERILYISRYKRPTYLDALPLDFGFAQVTTVVACGGYNSTLGFVSFFQKSVSANTFKVIEFKHFGTTRAIRDFWFTTKGIVSASSIRGSDKFLLHVSFHNKTLTRPSTRCALQLKNVGDEYIILDNELDLYLVKGKTTFKLASMAKFSSWNVCYMSAFQGSGSSSSLFIAISDGKDVYMLENGVIVAEYSFEDANINDICLKDCSSSKGTVLIVTNRAGCVTILNFSFETHSLSISLQVCLGENRAFRLCDIKMVESGTTEHQDSAFFVYSSSKVFVFNLSRRCFYKWIPKFDIKLIKYQGNSKYVVLTMDDSFVTLTYSGALSAEWTTEELVSKDWVYTKMVQLRNPRYILLASQTTNFKSKVSIFDTESLNTIDEYILPDQGVIKGVITLEKPYAQEILISFAGCSPESDCLLVLSALRNKLFKSGTYKTRGASAALTQRGETIACLGTTVLLFKLQRCLKKRVWDFKLLMRPFEGSGPWSVDCAFVSEEILCVLDRAKGICFFDLATKTPSLKCSIQSLAPDFANDRGHFDLLFSTLIEDTKQSEFYKINGPRTAKIRPLLDPIEKTARQLSIVVACGSQLLVYPCHPRYFKNSQNHSKCKLPCASGRIPFEDVVMSTRRTDANQFLVCTTGRLLITSLNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5E378</accession>
    <name>C5E378_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0H11022p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0H11022g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the WD repeat UTP18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556351.1">
        <property type="nucleotide sequence ID" value="XM_002556305.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E378"/>
    <dbReference type="FunCoup" id="C5E378">
        <property type="interactions" value="1155"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E378"/>
    <dbReference type="GeneID" id="8294682"/>
    <dbReference type="KEGG" id="lth:KLTH0H11022g"/>
    <dbReference type="eggNOG" id="KOG2055">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011055_1_0_1"/>
    <dbReference type="InParanoid" id="C5E378"/>
    <dbReference type="OMA" id="DLNRATY"/>
    <dbReference type="OrthoDB" id="1935146at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034388">
        <property type="term" value="C:Pwp2p-containing subcomplex of 90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000660">
        <property type="entry name" value="U3 snoRNP protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045161">
        <property type="entry name" value="Utp18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18359:SF0">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 18 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18359">
        <property type="entry name" value="WD-REPEAT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="3 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="241"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="552"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="41"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="172"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="45"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="102"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="179"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR30489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="582" mass="64983" checksum="9DBD31F8F9AA027F" modified="2009-07-28" version="1">MSAPVVHSQPVPPPDDEELMLSKLVFGDTSDFNENLSQLRSLDTEFGDADDVEMIEANSEEENDEDPEHNNMDAIQDDQLFFVDDGMTGEEQDKESAELGNGSDSASESDTGSEYYSEDSAVWSDSEDERVEVAFTTSNRTKKLRSSYSEGRIKGRDYVSRLRHQFEKIYPRPKWADSESSEEGPGSDDDSDREDREEVINGDLNALSRILQSTYNYRDTSSRLLPPSSLDITRLKDANAAHPSRAAVQSLSFHPSKPLLLTGGYDRTLRVYHIDGKNNHLVTSLYLTGTPIQTCSFYVSPSHQEQRIFTAGRRRYMHSWDISGTTPQQSRIEKISRMYGHEATQRSFEKFKLAHVCAQSGKAHGVILLQGNNGWVNVLHATTGVWMIGCKIEGVLVDFCVDYKPISNNKFQTILIMVNTYGEIWEFDLAKQGEVLRRWKDDGSVGVTTVQTGGGNNSTQLLPFKKIRHNRWLAIGSESGYVHVYDRNSSSAEPVAKLDQLTTPISSLAFSPDGQILCMASRATKDALRLVHLPTGKVFSNWPTSGTPLGKVTSVAFSPRGEMLAAGNEQGKVRLWRLNHYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3C8</accession>
    <name>C5E3C8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0H12276p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0H12276g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556401.1">
        <property type="nucleotide sequence ID" value="XM_002556355.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5E3C8">
        <property type="interactions" value="175"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3C8"/>
    <dbReference type="GeneID" id="8294738"/>
    <dbReference type="KEGG" id="lth:KLTH0H12276g"/>
    <dbReference type="eggNOG" id="ENOG502QSNK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001458_0_0_1"/>
    <dbReference type="InParanoid" id="C5E3C8"/>
    <dbReference type="OMA" id="VELQYKW"/>
    <dbReference type="OrthoDB" id="4066896at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057454">
        <property type="entry name" value="Bud3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021895">
        <property type="entry name" value="Bud3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12015">
        <property type="entry name" value="Bud3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25351">
        <property type="entry name" value="PH_BUD3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="2">
        <location>
            <begin position="263"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="781"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="863"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1201"/>
            <end position="1220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1251"/>
            <end position="1290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1321"/>
            <end position="1347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1366"/>
            <end position="1404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1447"/>
            <end position="1467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1489"/>
            <end position="1514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1529"/>
            <end position="1606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="781"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="884"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="913"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1203"/>
            <end position="1215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1251"/>
            <end position="1260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1278"/>
            <end position="1287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1322"/>
            <end position="1336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1375"/>
            <end position="1391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1547"/>
            <end position="1556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1595"/>
            <end position="1606"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR30539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1606" mass="179806" checksum="7A6F54C6F6CD4B06" modified="2009-07-28" version="1">MHNQELRNELSSLSSDPGGKPQERSGIKLRSNLFAGKAPNQWVLDRTESEWVESIFPDAAVFRGFDELLWGYFIIAVYKEPMPGKLSSLVVDKMGVTHFNSVDISRESRFHPAIENLTAEHQKSNVRKCIAASMLQKFSNLTPEMVAKIEPNMKYDYDPTVAGDLADSCQLVGFCSPADVGKSLGRLGFLQDRYVGSALLDVVYENKQETIDSNNELVFHLGEQLEQLFNPLTEYSPEQTESVYKPPEDDRDIEEEGSLIASICNELLQVQTNFTLVLVEFLQKFLIPLRIKASNEEIDALSIPKLNRLFPPTIDEVIRINCIFLDALKAATPHGSKEVLNACSVTIPYFYKAYTRHEAATKNFSKDIKLFMAKFRSVIPSCDVYTELRIETIMKGPQEIILKLKLILERLWSTKKWGNYDLKLAEARYSKVIEIIDSFGNVEQVLSSYSTRVFTPSGKILSELAKGWPVELQYKWLKRRVVGVFDVTDSNDATQKNILVIFSDFIVILKVLDDESYHDSNAGKKPLLSDILMNSLINEVPLPPKIPQLQVLCYSYIDNVFASTFDDKFIRIDYFDEEGANSIALQIISSSATASQVSDLVLKAKILEKDTAFHLFRYSNEELQIFSTAHEMRAYSTENIRSRFALFLNIEPSFSPIKQYNLAAAFFASISERKGVKINLVTIEGAKEEFDLALGELAEFLVQKLAELYPTYYSTASSPLLPQLLAINEQLVRRIGRHFHDSEHVGTSSQVAHSHIGTEAQASELHTKSFGTLTTFRSYTDDLKDSAPRSNDRQEISNRSGKQSKAHTQREGEQPANNGKAHRVNDKKAKRNSIISIFTNLFSRRKGSQYRTEHLDKVNDKGVGEDLLKGKFGGTEKSAVTRPASANKSQSRPQQKRLASVIHRPSLLQGAADPKHNTAKFKEDKETERRESNKKVVDQEVKVVPDSIAGASYDSGSHINSNPGVIEVQNRSSRLAALDYQEGKGVTVHDTSVLESDLASDKSACFRAENGTSMYALQEAGRESKVFNADLYGDVLMQEKGFESPDFLGHDPSKSNITQQAVGAQQQAKGIDVGKLVNEDQNSTTTPKILKTTQDKQKAANCNIVTSENEEPDFSPTKKDIFPEIQKLAIKNVLFDRSPSFRELFDSMRTVLDESDERSNWKILSSDPSVKEVAFSESKKTNETHAFKSLVPSNRPVADLKFSSSSEAQEEVSSVQHDDLETSEPLRLFGNISGLSSNIKADIAYRPSEIESTDKHKDVSGRSPFKVINHSPPKIIPLQSTPSISSKSGERFKTSDAFLSDISYSSLSGNVEPRLLELSFSSKEETDSKNRHESSLQKRGGKPPFNEQVFDLNVQQSANLFEKGNTTKIEFPDSNLKSTPANPSSALSSKKTAIPDSGPETNNVKQTCDATAEKNDFHEDQMSRCDLLDDPEFSTFHMTFDGLDEVQDSSDVPNFSKDNERTSTQKQIHSPEPLFYRFPATAKSNDTFFSCHDENSKSNPSGRQAAHHLTAEQDDPIWISPSKLEIFDTSMHPDVGSSKPRVQQDYYKNESEHKQSEFSPKSRRAPEEQALLSDSSYAYLGPLLADDNKSDESIDSQSPVRLQFHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3G8</accession>
    <name>C5E3G8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Ribosomal RNA-processing protein 44</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">KLTH0H13420g</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the RNR ribonuclease family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556441.1">
        <property type="nucleotide sequence ID" value="XM_002556395.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3G8"/>
    <dbReference type="FunCoup" id="C5E3G8">
        <property type="interactions" value="1216"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3G8"/>
    <dbReference type="GeneID" id="8294800"/>
    <dbReference type="KEGG" id="lth:KLTH0H13420g"/>
    <dbReference type="eggNOG" id="KOG2102">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002333_5_0_1"/>
    <dbReference type="InParanoid" id="C5E3G8"/>
    <dbReference type="OMA" id="GQVMRNN"/>
    <dbReference type="OrthoDB" id="372421at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071034">
        <property type="term" value="P:CUT catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071031">
        <property type="term" value="P:nuclear mRNA surveillance of mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09862">
        <property type="entry name" value="PIN_Rrp44-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000309">
        <property type="entry name" value="Exosome complex exonuclease DIS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000010">
        <property type="entry name" value="Exosome complex exonuclease DIS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.700:FF:000001">
        <property type="entry name" value="Exosome complex exonuclease exoribonuclease (Rrp44)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.690">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041505">
        <property type="entry name" value="Dis3_CSD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001900">
        <property type="entry name" value="RNase_II/R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022966">
        <property type="entry name" value="RNase_II/R_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050180">
        <property type="entry name" value="RNR_Ribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033771">
        <property type="entry name" value="Rrp44_CSD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033770">
        <property type="entry name" value="RRP44_S1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23355:SF35">
        <property type="entry name" value="EXOSOME COMPLEX EXONUCLEASE RRP44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23355">
        <property type="entry name" value="RIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17849">
        <property type="entry name" value="OB_Dis3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13638">
        <property type="entry name" value="PIN_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00773">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17216">
        <property type="entry name" value="Rrp44_CSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17215">
        <property type="entry name" value="Rrp44_S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00955">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01175">
        <property type="entry name" value="RIBONUCLEASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="11" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="10" id="KW-0271">Exosome</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="PIN" evidence="16">
        <location>
            <begin position="80"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="RNB" evidence="17">
        <location>
            <begin position="516"/>
            <end position="849"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00077930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAR30579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="986" mass="111725" checksum="84756A6B0987FC0F" modified="2009-07-28" version="1">MAIQRKRLSDGLTVTQKVFVRSRGGGATKIVREHYLRTDIPCLSKACKKCVEIAVPGPDNELPKFVLSAEPGELSTVGKHYVVLDANIVLQAIDLLENPTCFTDVIVPQIVLDEVRNRSYPVYTRLRTLCRDSDDKKRFTVFHNEFSEFTYVERSNDETINDRNDRAIRRTCVWFSEHLKPYGIHVVLVTNDRLNRELTEKDGIIAKSLLEYVDLLPNADIIKDALPNLEVNRSKDKDVEAKSEFKFPEYYSTARVMGGIRSGVLYQGSVQISEYNFLEGFVSLPNFSKPVLILGQKNLNRAFNGDQVVVELLPQGEWKAPSTVAIDSEHFDVNDNADEDEEENVEAEVISDKQRRLLAQDAILAQKSSKTQPTARVVSITRRSWRQYVGQIAPTSIDLQTGGTQNAFVILMDKCLPKIRIRTRRAKELLNKRIVIAVDSWPDTYKYPLGHFVRELGEIESVQAETEALLLEHDVEYRPFSKKVLDCLPAEGDDWKAPVDLSSPEAIAKDHLLPKRRDLRDKLICSIDPPGCVDIDDALHAKKLPNGNWEVGVHIADVTHFVKANTPLDAEGASRGTSVYLVDKRIDMLPMLLGTNLCSLKPYVDRFAFSVLWELDENANIVNVDFTKSVIKSREAFSYEQAQLRIDDESQKDELTQGMRALLQLSIKLKQKRLDAGALNLASPEVKVHMDSETSDPGEVEIKKLLATNSLVEEFMLLANISVARRIYEAFPQTAMLRRHAAPPATNFETLNEMLQLRKGMSISLESSKALANSLDRCVDLEDPYFNTLVRIMSTRCMMAAQYFYAGAYSYPDFRHYGLAVDIYTHFTSPIRRYCDVVAHRQLAAAIGYEPLDLNHRDKNKMDFMCRNINKKHRNAQFAGRASIEYYVGQVMRNNESVETGYVIRVFHNGIVVLVPKFGVEGLIRLENLTDDEPSAQFKAEEFKLSFTPKGAEQIKDVYIFDKVSVQVKSVLDPVTSKRKAQLLLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3H1</accession>
    <name>C5E3H1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Translation initiation factor IF-2, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0H13530g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556444.1">
        <property type="nucleotide sequence ID" value="XM_002556398.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3H1"/>
    <dbReference type="FunCoup" id="C5E3H1">
        <property type="interactions" value="529"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3H1"/>
    <dbReference type="GeneID" id="8294805"/>
    <dbReference type="KEGG" id="lth:KLTH0H13530g"/>
    <dbReference type="eggNOG" id="KOG1145">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006301_10_2_1"/>
    <dbReference type="InParanoid" id="C5E3H1"/>
    <dbReference type="OMA" id="RKNPWMN"/>
    <dbReference type="OrthoDB" id="361630at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01887">
        <property type="entry name" value="IF2_eIF5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03702">
        <property type="entry name" value="IF2_mtIF2_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03692">
        <property type="entry name" value="mtIF2_IVc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000126">
        <property type="entry name" value="Mitochondrial translation initiation factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000008">
        <property type="entry name" value="Translation initiation factor IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10050:FF:000001">
        <property type="entry name" value="Translation initiation factor IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000019">
        <property type="entry name" value="Translation initiation factor IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10050">
        <property type="entry name" value="Translation initiation factor IF- 2, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053905">
        <property type="entry name" value="EF-G-like_DII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044145">
        <property type="entry name" value="IF2_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006847">
        <property type="entry name" value="IF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000178">
        <property type="entry name" value="TF_IF2_bacterial-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015760">
        <property type="entry name" value="TIF_IF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023115">
        <property type="entry name" value="TIF_IF2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036925">
        <property type="entry name" value="TIF_IF2_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00487">
        <property type="entry name" value="IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381:SF20">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR IF-2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR IF-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22042">
        <property type="entry name" value="EF-G_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11987">
        <property type="entry name" value="IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04760">
        <property type="entry name" value="IF2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52156">
        <property type="entry name" value="Initiation factor IF2/eIF5b, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Tr-type G" evidence="12">
        <location>
            <begin position="134"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="440"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR30582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="672" mass="74607" checksum="557DDF210C84784C" modified="2009-07-28" version="1">MLRRLSIVHVTCRRLIPSVQQSPSLSWALGSTRNYAKRTSSRKNDLKPVTFKIPNYTSVSDLSNMTNIKIEKLIRDLTKMGFTNVTHNYILSREYVELVLSSYNYKVEEQTSVLTPETVYDELKSPVNPKNLMKRPPIVTIMGHVDHGKTTILDYLRKSSIVSKEHGGITQHVGAFQVETPISKRRITFLDTPGHAAFLKMRERGATITDIVILVISAEDSLMPQTLEALKHARNSGNQMVVAITKIDRFSNPKQREAAIEKVNLDMMNHEIPIERIGGDVQVVPISARTGENMELLEESIVLLSDVMDLKAETGAQATLEGYILESEMKKTTGNVSTILITKGELRKGKVLICGNTYCKVRSIKNENGQEVAKATPAQAVEITGWKDLPGAGDEVIEVKNEATAKKYVAKRVALIQTLKESENVDRLNEERATKVFSKLKKENENEDESLNSETGANAKDEGPKKVNFIVKGDVSGSVEAIVESIASLGNEEVRCNIVTASVGIPNENDMKMARITGSKLLCFNLGSLPHDIINNKDDIEVAQFNVIYKLIEDVTETLTDNLKPIYETKQVASAEIRDIFNFSVRKKTIKIAGCKVLSGQVSRNATVKIIRGADEKVVYDGTISSLKQGKDDASVVKKNNECGITFGNDFESYEPGDKVVVYEKVKVPRYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3H4</accession>
    <name>C5E3H4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0H13596p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0H13596g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556447.1">
        <property type="nucleotide sequence ID" value="XM_002556401.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3H4"/>
    <dbReference type="FunCoup" id="C5E3H4">
        <property type="interactions" value="100"/>
    </dbReference>
    <dbReference type="GeneID" id="8294808"/>
    <dbReference type="KEGG" id="lth:KLTH0H13596g"/>
    <dbReference type="HOGENOM" id="CLU_1461558_0_0_1"/>
    <dbReference type="InParanoid" id="C5E3H4"/>
    <dbReference type="OMA" id="CQNTHPE"/>
    <dbReference type="OrthoDB" id="4058540at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="134"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="167"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR30585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="201" mass="22853" checksum="02B23CE07CF81BC1" modified="2009-07-28" version="1">MSGNTDSGYSSSSEDFTKLEEAPKYRETPDLLNFGSSETSDKTKRANPLLRRRSTNYSDALEIERRLPAAVANDLSIIEGRGTKPMNEQFFKRDLQEKSENLRDGDAFYRYDCAQAGPKFVSAQDSSRDLDLMSQSQSQPRSQPRSQRPVNTNGITFCGEGNKSDVVDQSSSQRPSLYQRSSSKFEDYKKDIYDRLHLFNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3I9</accession>
    <name>C5E3I9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Enhancer of mRNA-decapping protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0H13948g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">P-body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EDC3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556462.1">
        <property type="nucleotide sequence ID" value="XM_002556416.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3I9"/>
    <dbReference type="FunCoup" id="C5E3I9">
        <property type="interactions" value="186"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3I9"/>
    <dbReference type="GeneID" id="8294824"/>
    <dbReference type="KEGG" id="lth:KLTH0H13948g"/>
    <dbReference type="eggNOG" id="KOG2585">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037134_0_0_1"/>
    <dbReference type="InParanoid" id="C5E3I9"/>
    <dbReference type="OMA" id="AMFDKKS"/>
    <dbReference type="OrthoDB" id="10030313at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031087">
        <property type="term" value="P:deadenylation-independent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033962">
        <property type="term" value="P:P-body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22576">
        <property type="entry name" value="Edc3_Lsm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10260">
        <property type="entry name" value="YjeF N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025762">
        <property type="entry name" value="DFDF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019050">
        <property type="entry name" value="FDF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004443">
        <property type="entry name" value="YjeF_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036652">
        <property type="entry name" value="YjeF_N_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13612">
        <property type="entry name" value="ENHANCER OF MRNA-DECAPPING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13612:SF0">
        <property type="entry name" value="ENHANCER OF MRNA-DECAPPING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09532">
        <property type="entry name" value="FDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03853">
        <property type="entry name" value="YjeF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01199">
        <property type="entry name" value="FDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64153">
        <property type="entry name" value="YjeF N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51512">
        <property type="entry name" value="DFDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51385">
        <property type="entry name" value="YJEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DFDF" evidence="7">
        <location>
            <begin position="104"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="YjeF N-terminal" evidence="6">
        <location>
            <begin position="246"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="62"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="69"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR30600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="497" mass="54212" checksum="769B731A237A310F" modified="2009-07-28" version="1">MSQFSGFGIEVELKDGKIITGRVAKANPKSLTLSDVTFSDGGTSQVFKVKASRLKDLKVVSTPKQGKQGVARNSSAGSVSGAAGAAAPAAARLASAGSNTDWQDDDAAAIKQQDDFDFQENLRMFNKEDVFAKLKEQDKVEQKSRLVSHNIKDRKFETDELVIPDAKQDNWDSLEEDSTHTEYLPITKSINITHLLQSAGDNEAKNDQVLSQLQRALTRPGSISKPSGFVTTTTGVSVPCATPVQLLEIERVCSDSFGFTSKMAMEHWAVHMSLFVKQKLGGRVRLHAQNTNPQPLVVILASDNRCGARALALGRVLCQSSLVRVLVLFTNTPEDPEVNEQWQLYQKSGGKTVESLQSLKSNLEKLNSPVEIVLDAMQGFDLNLQDLCSDAADEARLEDIINWCNAQQAVWSLDIPSGLDAGSALPNFSAHVNANSVICLCWPLTGAYNLRSQFPNHAPEIHLVDLGVPQAVYSVRSSLRKFQIADLFVTEGMVQLT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1995-11-01" modified="2026-01-28" version="131" xmlns="https://uniprot.org/uniprot">
    <accession>P43376</accession>
    <accession>Q5K459</accession>
    <name>COX2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName>Cytochrome c oxidase subunit 2</fullName>
            <ecNumber>7.1.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Cytochrome c oxidase polypeptide II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">COX2</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <geneLocation type="mitochondrion"/>
    <reference key="1">
        <citation type="journal article" date="1994" name="J. Mol. Evol." volume="38" first="593" last="601">
            <title>The structure of the small mitochondrial DNA of Kluyveromyces thermotolerans is likely to reflect the ancestral gene order in fungi.</title>
            <authorList>
                <person name="Clark-Walker G.D."/>
                <person name="Weiller G.F."/>
            </authorList>
            <dbReference type="PubMed" id="8083884"/>
            <dbReference type="DOI" id="10.1007/BF00175879"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>CBS 2803</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="FEBS Lett." volume="579" first="30" last="40">
            <title>The complete mitochondrial genome of the yeast Kluyveromyces thermotolerans.</title>
            <authorList>
                <person name="Talla E."/>
                <person name="Anthouard V."/>
                <person name="Bouchier C."/>
                <person name="Frangeul L."/>
                <person name="Dujon B."/>
            </authorList>
            <dbReference type="PubMed" id="15620687"/>
            <dbReference type="DOI" id="10.1016/j.febslet.2004.10.106"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>4 Fe(II)-[cytochrome c] + O2 + 8 H(+)(in) = 4 Fe(III)-[cytochrome c] + 2 H2O + 4 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:11436"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="7.1.1.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="2">
            <dbReference type="Rhea" id="RHEA:11437"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="2">Binds a dinuclear copper A center per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the cytochrome c oxidase (complex IV, CIV), a multisubunit enzyme composed of a catalytic core of 3 subunits and several supernumerary subunits. The complex exists as a monomer or a dimer and forms supercomplexes (SCs) in the inner mitochondrial membrane with ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="1">The signal sequence of COX2 is processed by IMP1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cytochrome c oxidase subunit 2 family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.9"/>
    <dbReference type="EMBL" id="X69431">
        <property type="protein sequence ID" value="CAA49206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AJ634268">
        <property type="protein sequence ID" value="CAG25605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S45322">
        <property type="entry name" value="S45322"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_184727.1">
        <property type="nucleotide sequence ID" value="NC_006626.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P43376"/>
    <dbReference type="SMR" id="P43376"/>
    <dbReference type="FunCoup" id="P43376">
        <property type="interactions" value="246"/>
    </dbReference>
    <dbReference type="STRING" id="559295.P43376"/>
    <dbReference type="GeneID" id="3239005"/>
    <dbReference type="InParanoid" id="P43376"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Mitochondrion"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd13912">
        <property type="entry name" value="CcO_II_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.90:FF:000004">
        <property type="entry name" value="Cytochrome c oxidase subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000001">
        <property type="entry name" value="Cytochrome c oxidase subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045187">
        <property type="entry name" value="CcO_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002429">
        <property type="entry name" value="CcO_II-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034210">
        <property type="entry name" value="CcO_II_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001505">
        <property type="entry name" value="Copper_CuA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014222">
        <property type="entry name" value="Cyt_c_oxidase_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011759">
        <property type="entry name" value="Cyt_c_oxidase_su2_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036257">
        <property type="entry name" value="Cyt_c_oxidase_su2_TM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02866">
        <property type="entry name" value="CoxB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22888:SF9">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22888">
        <property type="entry name" value="CYTOCHROME C OXIDASE, SUBUNIT II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00116">
        <property type="entry name" value="COX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02790">
        <property type="entry name" value="COX2_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01166">
        <property type="entry name" value="CYCOXIDASEII"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81464">
        <property type="entry name" value="Cytochrome c oxidase subunit II-like, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00078">
        <property type="entry name" value="COX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50857">
        <property type="entry name" value="COX2_CUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50999">
        <property type="entry name" value="COX2_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0186">Copper</keyword>
    <keyword id="KW-0249">Electron transport</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0496">Mitochondrion</keyword>
    <keyword id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0679">Respiratory chain</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000006040" description="Cytochrome c oxidase subunit 2">
        <location>
            <begin position="16"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="topological domain" description="Mitochondrial intermembrane" evidence="3">
        <location>
            <begin position="16"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="topological domain" description="Mitochondrial matrix" evidence="3">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="83"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="topological domain" description="Mitochondrial intermembrane" evidence="3">
        <location>
            <begin position="104"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
            <label>A1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
            <label>A1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
            <label>A2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
            <label>A2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>ligand shared with subunit 1</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
            <label>A1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
            <label>A2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
            <label>A2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
            <label>A1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="P00410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3"/>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="251" mass="28476" checksum="02A2A93B463996F2" modified="1995-11-01" version="1" precursor="true">MLNLLYNQIFNVILNDVPTPYNTYFQDSATPNQEGILELHDNIMFYLLVILGLVSWLLFTITRTYSKNPIAYKYIKHGQTIEIIWTIFPAVILLIIAFPSFILLYLCDEVISPAMTIKAIGLQWYWKYEYSDFINDSGETVEFESYVIPEDLLEDGQLRLLDTDTSVVVPVDTHIRFVVTAADVIHDFAIPSLGIKVDAAPGRLNQVSALIQREGVFYGQCSELCGTAHSAMPIKIEAVSLPAFLEWLNEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBA9</accession>
    <name>C5DBA9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0A01034p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0A01034g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551508.1">
        <property type="nucleotide sequence ID" value="XM_002551462.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBA9"/>
    <dbReference type="GeneID" id="8290299"/>
    <dbReference type="KEGG" id="lth:KLTH0A01034g"/>
    <dbReference type="eggNOG" id="KOG2533">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_0_1_1"/>
    <dbReference type="InParanoid" id="C5DBA9"/>
    <dbReference type="OMA" id="GFTTMMQ"/>
    <dbReference type="OrthoDB" id="2985014at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000013">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000057">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF46">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="126"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="152"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="186"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="219"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="290"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="331"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="355"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="380"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="415"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="448"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="60"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR21066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="506" mass="56438" checksum="43D7348075FBC0E5" modified="2009-07-28" version="1">MSEAKLEALATVGTSEKASTVSVTSRRSYNGDELSEIQQDLAQKHGVKQRKLEWKIDICVVPALVLLYFAAFLDRINISNARLYGMEEDLGLHGNQFNVALTVFFVPYIVFELVANYLAKLFKPHWCFSVSMFMFGCSTIGCGFVKSFGGLITVRVFLGVAESATFGLIFYILSSYYTPRQAQRRFSCFFSSVTLAGAAGGSIARQIYTGIGNKHLANWRWIFIIEGAATVFIAFLLFFITPDFPETAKFLKPNEKAFLKEKLQLYSRVESGFEVKFNWRDYLPLFKEPIFYANALAYLGLLVPSYGYAYFATSIIKLLGYTAVSAQQHSVYPWLAAFGYINIVAYLSDRLQNRWCFSVSSSIIGIAGLAMVLGSSAPHVRYGGCFVASLGLYGATPVVVCWTSINLGGHLRKSFGTAFQVGLGNIGGIIATFLFLQKDEPKYTSGLSVSIGFVGLSIISQLFIVFYCSFMNKKKMTLTYIERFEELPIEDRVLKGDLNPNFKYLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBC5</accession>
    <name>C5DBC5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0A01408p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0A01408g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Bud neck</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551524.1">
        <property type="nucleotide sequence ID" value="XM_002551478.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBC5"/>
    <dbReference type="FunCoup" id="C5DBC5">
        <property type="interactions" value="232"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBC5"/>
    <dbReference type="GeneID" id="8290316"/>
    <dbReference type="KEGG" id="lth:KLTH0A01408g"/>
    <dbReference type="eggNOG" id="KOG2058">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005350_12_2_1"/>
    <dbReference type="InParanoid" id="C5DBC5"/>
    <dbReference type="OMA" id="YFARGQE"/>
    <dbReference type="OrthoDB" id="294251at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005934">
        <property type="term" value="C:cellular bud tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000131">
        <property type="term" value="C:incipient cellular bud site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000133">
        <property type="term" value="C:polarisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070649">
        <property type="term" value="P:formin-nucleated actin cable assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000057">
        <property type="entry name" value="GTPase-activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000036">
        <property type="entry name" value="GTPase-activating protein GYP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF9">
        <property type="entry name" value="GTPASE ACTIVATING PROTEIN AND CENTROSOME-ASSOCIATED, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="6">
        <location>
            <begin position="200"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="29"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR21082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="566" mass="65219" checksum="6B4B04276F947C11" modified="2009-07-28" version="1">MTKLSDVHDYGSSKTKSRNGGLSGYLASKSPSVPNLTQTFTTRSMKETSPERAGYQQFGSPLVEEPTNLSVIDLYGDEDDEDADDDEDADDDEAEGSQSTSGVIADLPVSSTESPSLEAPNAFGAAKDLSQYDRYGFKKQTAHVSQEEYHMWWDQYSRYCIRRKRKWQDLLEKSGLAPSNDVPDRFPPRSEKLKRYVRKGIPAEWRGQAWWYFARGQEKLNKNAGVYDKLLEGLNNKKDKNNCKDLEVIERDLHRTFPENIHFQKDPKEGQEPLMIQSLRRVLLAFSVYNPKIGYCQSMNFLAGLLLLFLDEEKSFWMLVIITSRYLPGVHNVNLEGVNVDQGVLMLCVKEYLPDFWQRIIPKEQSRSNEFLYKLPPITLCTASWFMSCFVGVVPIETTLRIWDCLFYEESHVLFKISLSIIKICEQELAGSRLAHRGLRARTHGRDDDDIEVFQVIQTVPKKLLDPQDIFDRIIFKRRVPLNNLNQEEIDRCRKYVTSQRLKFKNCAEIMGDRTSAKASAKRDLEIGPDFVNNALSSEVYGFRRSLTGAHWNNSIKEKVRQIRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBI5</accession>
    <name>C5DBI5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Efficient mitochondria targeting-associated protein 19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0A02904g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM97/sigma-2 receptor family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551584.1">
        <property type="nucleotide sequence ID" value="XM_002551538.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBI5"/>
    <dbReference type="FunCoup" id="C5DBI5">
        <property type="interactions" value="48"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBI5"/>
    <dbReference type="GeneID" id="8290383"/>
    <dbReference type="KEGG" id="lth:KLTH0A02904g"/>
    <dbReference type="eggNOG" id="ENOG502S75H">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086812_4_0_1"/>
    <dbReference type="InParanoid" id="C5DBI5"/>
    <dbReference type="OMA" id="EFKDPMV"/>
    <dbReference type="OrthoDB" id="433124at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033118">
        <property type="entry name" value="EXPERA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051987">
        <property type="entry name" value="Sigma-2_receptor-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016964">
        <property type="entry name" value="Sigma2_recept"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31204">
        <property type="entry name" value="SIGMA INTRACELLULAR RECEPTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31204:SF1">
        <property type="entry name" value="SIGMA INTRACELLULAR RECEPTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05241">
        <property type="entry name" value="EBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF031032">
        <property type="entry name" value="TMP_97_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51751">
        <property type="entry name" value="EXPERA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="9"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="110"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="149"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="EXPERA" evidence="9">
        <location>
            <begin position="6"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR031032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR21142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="168" mass="19992" checksum="7FAC895F5467AF74" modified="2009-07-28" version="1">MLSNGLQKFYYYYFIIHIFTTILIDSSVILPAKFQFTQPLVEWHIAQNNDFLLFEKPDWLWYFVLIECVGQLPGFFWFAGKFRQLWSLKESQTKADKMAIRDCEKSLSKWLRVYGWNASVTTLICLWTVWTRGYYPSGEFLPMNVQDKIKLMAIYVPYVLIPLRLCLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBP2</accession>
    <name>C5DBP2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0A04246p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0A04246g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PET117 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551641.1">
        <property type="nucleotide sequence ID" value="XM_002551595.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBP2"/>
    <dbReference type="FunCoup" id="C5DBP2">
        <property type="interactions" value="36"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBP2"/>
    <dbReference type="GeneID" id="8290444"/>
    <dbReference type="KEGG" id="lth:KLTH0A04246g"/>
    <dbReference type="eggNOG" id="ENOG502S49F">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_161486_0_0_1"/>
    <dbReference type="InParanoid" id="C5DBP2"/>
    <dbReference type="OMA" id="VHYVQEL"/>
    <dbReference type="OrthoDB" id="76305at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031568">
        <property type="entry name" value="Pet117"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28163">
        <property type="entry name" value="PROTEIN PET117 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28163:SF1">
        <property type="entry name" value="PROTEIN PET117 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15786">
        <property type="entry name" value="PET117"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="52"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="52"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="67"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="77"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="110"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR21199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="122" mass="13769" checksum="21650203B2365030" modified="2009-07-28" version="1">MKSQYIRRTNIFRHTMSTASKITLALSCAVTAATVVGVHYVQELERETLHQGPIKDAKRMAERKAEQSAGAGAVSAESERKQHFNRSEHELQQELRKKYEAMQPLSGEVLTKDGERAARDEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBQ2</accession>
    <name>C5DBQ2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">Isocitrate lyase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0A04466g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle, which operates as an anaplerotic route for replenishing the tricarboxylic acid cycle. Required for growth on ethanol or acetate, but dispensable when fermentable carbon sources are available. Also acts on 2-methylisocitrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = pyruvate + succinate</text>
            <dbReference type="Rhea" id="RHEA:16809"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57429"/>
            <dbReference type="EC" id="4.1.3.30"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>D-threo-isocitrate = glyoxylate + succinate</text>
            <dbReference type="Rhea" id="RHEA:13245"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="EC" id="4.1.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="13">Can also use Mn(2+) ion.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551651.1">
        <property type="nucleotide sequence ID" value="XM_002551605.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBQ2"/>
    <dbReference type="FunCoup" id="C5DBQ2">
        <property type="interactions" value="204"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBQ2"/>
    <dbReference type="GeneID" id="8290454"/>
    <dbReference type="KEGG" id="lth:KLTH0A04466g"/>
    <dbReference type="eggNOG" id="KOG1260">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019214_2_2_1"/>
    <dbReference type="InParanoid" id="C5DBQ2"/>
    <dbReference type="OMA" id="YVSGWQV"/>
    <dbReference type="OrthoDB" id="4078635at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004451">
        <property type="term" value="F:isocitrate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046421">
        <property type="term" value="F:methylisocitrate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006097">
        <property type="term" value="P:glyoxylate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00377">
        <property type="entry name" value="ICL_PEPM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.850:FF:000001">
        <property type="entry name" value="Isocitrate lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.850">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039556">
        <property type="entry name" value="ICL/PEPM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006254">
        <property type="entry name" value="Isocitrate_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018523">
        <property type="entry name" value="Isocitrate_lyase_ph_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01346">
        <property type="entry name" value="isocit_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21631:SF3">
        <property type="entry name" value="BIFUNCTIONAL GLYOXYLATE CYCLE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21631">
        <property type="entry name" value="ISOCITRATE LYASE/MALATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00463">
        <property type="entry name" value="ICL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001362">
        <property type="entry name" value="Isocit_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00161">
        <property type="entry name" value="ISOCITRATE_LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0329">Glyoxylate bypass</keyword>
    <keyword evidence="7 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="217"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="106"/>
            <end position="108"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="218"/>
            <end position="219"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="438"/>
            <end position="442"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="472"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00057462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR001362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001362-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR001362-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR001362-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR21209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="556" mass="62339" checksum="4E46C1BAACB82BDB" modified="2009-07-28" version="1">MTVPTTQNLDSFESLQRQLEQQTAEIEKWWSQPRWKHTKRIYSAREIAARRGTMPMAPHASSTMAQKLYKLLEEHHENRTVARTFGALDPVHVTQMAKYLDTVYVSGWQCSSTASTSNEPGPDLADYPMDTVPNKVDHLFKAQLFHDRKQWEDRSRCKSQAELDKLGPATDYLRPIVADADAGHGGVTAVFKLTKMFIEKGAAGIHMEDQTSTNKKCGHMAGRCVIPVQEHISRLVTIRMCADIMHSDLVLVARTDSEEATLLSSTIDPRDHYFIVGATNPDVEVSLAETLEAAIMKGVSAADLVATEERWCAKAGLKLFHEAFADEVRKHETSNQEQIIKEFNETVGPLAGFSLREMQAVAKKLLGKPLHFDWDLPRAREGLYRYRGGTQSSIMRARAFAPYADLVWMECNSPDYEQAKAFAEGVKAKFPDQWLAYNLSPSFNWTKTMDAKQLRTFIDRLGDLGYIWQFITLAGLHTSALAVNAFSKDFAERGMQSYAETVQQKEIDQGVDILKHQKWSGAEYIDSLLKLAHGGTSATAAMGLGVTEDQFHENGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBY4</accession>
    <name>C5DBY4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0A06314p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0A06314g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551733.1">
        <property type="nucleotide sequence ID" value="XM_002551687.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBY4"/>
    <dbReference type="FunCoup" id="C5DBY4">
        <property type="interactions" value="852"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBY4"/>
    <dbReference type="GeneID" id="8290538"/>
    <dbReference type="KEGG" id="lth:KLTH0A06314g"/>
    <dbReference type="eggNOG" id="KOG2570">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032070_0_0_1"/>
    <dbReference type="InParanoid" id="C5DBY4"/>
    <dbReference type="OMA" id="NFRCNEP"/>
    <dbReference type="OrthoDB" id="10263741at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="CDD" id="cd10568">
        <property type="entry name" value="SWIB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.245.10">
        <property type="entry name" value="SWIB/MDM2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036885">
        <property type="entry name" value="SWIB_MDM2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13844">
        <property type="entry name" value="SWI/SNF-RELATED MATRIX-ASSOCIATED ACTIN-DEPENDENT REGULATOR OF CHROMATIN SUBFAMILY D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47592">
        <property type="entry name" value="SWIB/MDM2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="440" mass="49707" checksum="0B1449BC8551E30A" modified="2009-07-28" version="1">MSKPRKQSIQPQANQRTTPLPITQPTDAYIPPFLVEQVPELKSYKQLLEAESKIDIYISRKKIDLYQSVSQWNSLKQEAQEKQYLRIFVSNIAENQAWQTNDANAQPSWTLRIEGRLLNDLDVNDPQRPKFSSFLQGIAVDFTRPQNADAGAQDQQQPQPLVPSIVEWHADPSALAEFDGLDVKRDGAENVKCRIAIQPRGVTGEWIQFSPELSSIIGMSRGTLHEAVYSLYKYILINDLLTSEDDVQAPGNPSSNSTNGERTITKIDKYLAALFPDERKTMRLAEIPSLVNRHISPLPPIRIDYTIRVDKASTYGETVIDIEVPAVSEDEVAREGMSLLTELNQLSTTLEPQMQALNQELNILQLQLNASANKYQFFSKLSKDPVPMLQEYMDSSSRALKVLSGDDGFNEDTVRRAQFYKDNEAMLFENLSVLLSNGRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>C5DC21</accession>
    <name>C5DC21_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0A07172p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0A07172g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551770.1">
        <property type="nucleotide sequence ID" value="XM_002551724.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DC21"/>
    <dbReference type="FunCoup" id="C5DC21">
        <property type="interactions" value="13"/>
    </dbReference>
    <dbReference type="GeneID" id="8290577"/>
    <dbReference type="KEGG" id="lth:KLTH0A07172g"/>
    <dbReference type="eggNOG" id="ENOG502RZCP">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081665_0_0_1"/>
    <dbReference type="InParanoid" id="C5DC21"/>
    <dbReference type="OMA" id="RFYIVNR"/>
    <dbReference type="OrthoDB" id="4083656at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR21328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="276" mass="30200" checksum="C2AC0BAEFADCF49E" modified="2009-07-28" version="1">MFRPAALRFVPRTTWRGAAHSTAGLPFSSKLAALIAKTSPEQIYTYSVPRLVKFGSWTLSGVFLVYGVSFADWSLTSSLELYGEEVQQPPSSWWKHPKLLLAARVAGSALLTLIPLTLSCVAIYAPSRIVTAVSYVPHGACKLTRGALLTGKPVSRVADLSHVARNQKAKVYTGVGPQGTDDRASFAFLLTDSHRPAWDRFYIVNRSGRFWAQDGRIFDALFGGESARSLECPSATQTTGPPPATSPVQRMIEVEQTRSKIHDHASRVKSIVMKPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCH1</accession>
    <name>C5DCH1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0B03014p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0B03014g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551920.1">
        <property type="nucleotide sequence ID" value="XM_002551874.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCH1"/>
    <dbReference type="FunCoup" id="C5DCH1">
        <property type="interactions" value="26"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCH1"/>
    <dbReference type="GeneID" id="8290752"/>
    <dbReference type="KEGG" id="lth:KLTH0B03014g"/>
    <dbReference type="eggNOG" id="KOG1437">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008441_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCH1"/>
    <dbReference type="OMA" id="FCSSNKR"/>
    <dbReference type="OrthoDB" id="286301at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.180.10">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050904">
        <property type="entry name" value="Adhesion/Biosynth-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036378">
        <property type="entry name" value="FAS1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000782">
        <property type="entry name" value="FAS1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10900:SF125">
        <property type="entry name" value="FAS1 DOMAIN-CONTAINING PROTEIN YLR001C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10900">
        <property type="entry name" value="PERIOSTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02469">
        <property type="entry name" value="Fasciclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82153">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50213">
        <property type="entry name" value="FAS1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950415" evidence="3">
        <location>
            <begin position="23"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="752"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="4">
        <location>
            <begin position="34"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="4">
        <location>
            <begin position="159"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="4">
        <location>
            <begin position="601"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="787"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="799"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="837"/>
            <end position="857"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR21482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="857" mass="96673" checksum="813BC8111FFDB581" modified="2009-07-28" version="1">MINIIWLAISVLWLKLLPFSTATPKSGDDDDFPFTTVVDYLWQNAEFSTFLRVLQKDGLIPYLNELDNFTIFAPINSAFAQSNQNTVLDHYLLQNHVLDFEKYGSGTHVLTLNDSRPHLIEKRTDGAWKINGVSVLKEILKPSMQNAVLYSITSLISGTLELEDLIEKSANISSFSMLLDRVVFGNLQVFDNKTLIIPKDSAFNASFNDIELEYLLGPTDTAISNPHLLKTYKNRKKDQNFLFERLLVDGILGGDINKTDALNLDNELLNFESHENGTVISVNNTFSALSNQLYTKGIAHMFSKLDFLPSHLSFTAEKYLIGLGADRFVEEIYMRKLDHLINGAHDRNLTIFVSVESSNEGSGFSKSSLLYHFVDDQVDLTDYALPTSSKRLYDTMFCSSNKRLGGECQRLKIERSSRGKQRVLINGKYCLKSLEPIKVGNTSIYLLENDVTLPADLLSSIDPFSGCSKSLHFLDELNLLELRPNNQGYTVLLPCFDSWDYFDLNLDYLEHNITALNLIMKNYILNGLIYTDSKEQTLETRNFYNELIQVEILGRESDSSQFAMNLSTVAGPVTLKNGADTFFDRGVLHPMERVFFPKSLDITLLNLLETTKSFEFLSFLENFPEFDNIVHQGKPSSILVPTSQSLLLDDISLDSSRLKEFLELHIIPANSTEALLNCQSNIQTLHGEYLQCRESSSNTHLLRFKSGLDKEVRVLRKGCASTDNNTCVFVIDQPLSLSWLDQARYQIGLPGIAFAVGMLLGVLGIFSLLSCTLMLFMRRRNVEDRGSGQVYNEQEGLLSSQPQPQQPYSSMNMSTPPPTAAQRNSSKNDTNSRSRIFENSYSMHSSRNPVSIQQNRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCH7</accession>
    <name>C5DCH7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0B03168p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0B03168g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ORC3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551926.1">
        <property type="nucleotide sequence ID" value="XM_002551880.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCH7"/>
    <dbReference type="FunCoup" id="C5DCH7">
        <property type="interactions" value="974"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCH7"/>
    <dbReference type="GeneID" id="8290759"/>
    <dbReference type="KEGG" id="lth:KLTH0B03168g"/>
    <dbReference type="eggNOG" id="KOG2538">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_403318_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCH7"/>
    <dbReference type="OMA" id="CPTFMFF"/>
    <dbReference type="OrthoDB" id="10265211at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031261">
        <property type="term" value="C:DNA replication preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005664">
        <property type="term" value="C:nuclear origin of replication recognition complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005656">
        <property type="term" value="C:nuclear pre-replicative complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20704">
        <property type="entry name" value="Orc3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020795">
        <property type="entry name" value="ORC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045667">
        <property type="entry name" value="ORC3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040855">
        <property type="entry name" value="ORC_WH_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12748">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12748:SF0">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07034">
        <property type="entry name" value="ORC3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18137">
        <property type="entry name" value="WHD_ORC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Origin recognition complex subunit 3 N-terminal" evidence="6">
        <location>
            <begin position="4"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="Origin recognition complex subunit 3 winged helix C-terminal" evidence="7">
        <location>
            <begin position="483"/>
            <end position="627"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF18137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR21488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="630" mass="73300" checksum="D7ADD9B4644AC709" modified="2009-07-28" version="1">MSLNEFAEAQKTHYTVCPKVKRRRISCEKGIDPFVKLLAGKESMQMVNKRWVLYHQLHSQFHAQVEEIVHRVSIDMYREISSVLSKDTSKRTSPSFNCLFLLGSDSTTTIELHEQNSKTMQAIIDLTPKESPNVRMMLRRSMFKLLSATEAPLRVKTDEKESFAPEGDMGELPRTSPLGISYDLSLVQNFRKLFGKRLKLIFNFKDVDSFNSQVLDDFIILLKSALNYKDVEICLVLNINTNLSNIQKNLRQSTIRLLKKDFTVIDLSHNKGHKYANQTFQSFLNTVDGKLNLSAEFIKFILDKMSNNATHNLQLLIKILDYSLMSYFFQNPFSVFIDPTNVVNFDENYLPPLLKCPTFMTFVEGMVHAHAPSEEIMALLDNKNSTLEEFFTEFLVRENPINDHLKLVVDVLENQLGVFNYNLIDLYYHMLTGKLHEFLQRWPECQQFEDILKFEPVDIIFQELFTLDNSKELFSQSLFPFLRTNLENNLINSDRMLPPAKRLPGIKDPFEAEFNSSVDLPLCQLFSLYREASSIINLYDFFLAFKETLPRSEIANFVNYSVENKESDLRHEDREKLSILLKEKGLDNLLDKMMLVWFVQSISEFQHMGLLKTQSNKSYEVVEKCIWRGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCM8</accession>
    <name>C5DCM8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0B04378p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0B04378g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551977.1">
        <property type="nucleotide sequence ID" value="XM_002551931.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCM8"/>
    <dbReference type="FunCoup" id="C5DCM8">
        <property type="interactions" value="36"/>
    </dbReference>
    <dbReference type="GeneID" id="8290814"/>
    <dbReference type="KEGG" id="lth:KLTH0B04378g"/>
    <dbReference type="eggNOG" id="ENOG502QWER">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_647344_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCM8"/>
    <dbReference type="OMA" id="QRGGPEY"/>
    <dbReference type="OrthoDB" id="4032461at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="380" mass="44093" checksum="6A24049140004A4E" modified="2009-07-28" version="1">MKESIGPTCRCSKCNSLLKASPKATAKKPIRYRFNFMKHMDLRQSNYRTITSISYLTEKYGEKKAENIARFVRCIHKQINSGVNRISDLQASSILPWTRVRLFLLLVTLSQRGGSDYWMGKEGEMKEPARPSEPRERKPQDKSKPYGTLIEEIMRQNINDNYREADHDENYVFSSIWANFMEGLINHFLEKVIIPSSERKVCQQLYKPMMKIISLYNEYNELMEKSERNGFLPPNQEPPAIISSDEATSIEDGKLQTAQRLLWQARQDIPKTISKELTLLSEMYSTLSADEQDFELDEFVCSAEEYIELEYLPSLIEVLFANGGTLNFWKIMIVLEPFFYYIEEVADEESPCYDGDSAKPDPRVVTLEKICEVAAEQEWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2023-02-22" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCP2</accession>
    <name>C5DCP2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0B04686p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0B04686g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551991.1">
        <property type="nucleotide sequence ID" value="XM_002551945.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCP2"/>
    <dbReference type="GeneID" id="8290828"/>
    <dbReference type="KEGG" id="lth:KLTH0B04686g"/>
    <dbReference type="HOGENOM" id="CLU_2250625_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCP2"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR21553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="104" mass="11772" checksum="5773F34015915922" modified="2009-07-28" version="1">MRYAFYEEHAPQLRSSRTTIVSNEDTWLSYKDAILDNINAPEKSQFENAYARELSRLESVGTWDATAPRPAHSLAPEAIIDTNFEFNTNSNGEKCCRVVQEGVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCZ7</accession>
    <name>C5DCZ7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0B07106g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552096.1">
        <property type="nucleotide sequence ID" value="XM_002552050.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCZ7"/>
    <dbReference type="FunCoup" id="C5DCZ7">
        <property type="interactions" value="322"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCZ7"/>
    <dbReference type="GeneID" id="8290936"/>
    <dbReference type="KEGG" id="lth:KLTH0B07106g"/>
    <dbReference type="eggNOG" id="KOG0590">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_82_4_1"/>
    <dbReference type="InParanoid" id="C5DCZ7"/>
    <dbReference type="OMA" id="LQVMEYC"/>
    <dbReference type="OrthoDB" id="6513151at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13994">
        <property type="entry name" value="STKc_HAL4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000714">
        <property type="entry name" value="Nitrogen permease regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000919">
        <property type="entry name" value="NPR1p Protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343:SF113">
        <property type="entry name" value="NITROGEN PERMEASE REACTIVATOR PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343">
        <property type="entry name" value="SERINE/THREONINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="396"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="81"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="250"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="339"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="624"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="732"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="44"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="105"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="126"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="137"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="157"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="251"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="288"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="343"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="652"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="425"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR21658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="751" mass="80621" checksum="681FFD5C025F414D" modified="2009-07-28" version="1">MSSLTKLLQEKRKNEGGLSSGLAQTRSQMSSAGTAAGTSGGTGSAASEELSSSVGSNVLMNQEAFSSSFFNANSAHTAALSESYKDRDRPAPLTIESRGGNARLSSQGFQPGSQPGPQSGPRPVPSLSSSIPYSVPNSNANGSSGSNAAGGAGSGGFHSSNGSGVSSASSSWLESPGVMPSNVSAIDSNVISSPKVDTVEPRFVISKQKFQKASMDNAANSYSNAQNGSISRSSSISSQLGNLFFTKNGKELSSSQNSQSRDIPTNSAQSAAYGSSIPKPMRARRSSLYGSSRQPSNSYQDTFIGSPSSLHEQPPNQYMPRSRHSSIADLKRFFKKGQSVNMSVSPGTPPVSSGGNISSHNSNASSFGPGSYSSNNGDTIYTSSASSTLPYSKRYTKTGEVLGAGAGGSVRLVKRLQDNKVFAVKEFRTKFESEAKRDYVKKITSEYCIGTTLRHPNIIETVEIVYDNNRILQVMEYCDYDLFAIVMSNKMSYEEICCCFKQILTGVQYLHSMGLAHRDLKLDNCVINKQGVVKLIDFGAAAVFSYPFSKTLVESSGIVGSDPYLAPEVCIFSKYDPRPVDVWSIAIIFACMVLKKFPWKIPKLKDNSFKLFCSGRNCDSLSALVTRTPDPPSYDSATNTDENDKPKAEAPVSNNPANPENPNIGPQRLLHSLPEESQHIIGRMVELAPACRVSIDDIMKDPWVESIDVCEVVEEGYSTKVVSGVDHKHTQVDQSEAHIAGLEKKKKKQNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-04-02" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>C5DD14</accession>
    <name>C5DD14_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0B07502p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0B07502g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552113.1">
        <property type="nucleotide sequence ID" value="XM_002552067.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DD14"/>
    <dbReference type="GeneID" id="8290953"/>
    <dbReference type="KEGG" id="lth:KLTH0B07502g"/>
    <dbReference type="HOGENOM" id="CLU_299969_0_0_1"/>
    <dbReference type="InParanoid" id="C5DD14"/>
    <dbReference type="OMA" id="HAGRQKH"/>
    <dbReference type="OrthoDB" id="4036528at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="180"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="222"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="257"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="298"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="329"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="367"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="390"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="414"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="503"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="519"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="573"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="662"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="724"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="740"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="799"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="810"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="858"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="904"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="943"/>
            <end position="977"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="999" mass="107444" checksum="BBCFB605FB8A5E79" modified="2009-07-28" version="1">MVMIRNSHKDESSTTQDYESSRQNVSAQGNTSTTATRDTSSGSSNNPFAPQRAVRGIKAFDVGSEHNSTTQEHSSQTQEYGSHQSQHSQRQAPVSSSSGGNGQPSDDSSRGAQQPHFHRADAPVAGVPGVRNHQSGPSLEESSHSQGHGGFLSSTGHHERHEQGSTHQAKSQEIYGHQSGAPSTSAQGNDDSLRGFQHTSGHQHGTEQAVLGTAPVSHSKHTTGVTSGANLEQETGARNPQQGHTSNESAGHDTNRPSDNSYSKSTETRAPQTGPRTTEKGTSFSTTLPIGALGQERSTSSPTNPTESSDQHKRGVFRLKSSKSGTETSGKESEGRKNSEGRKNSLIQEAIGLKNKINEQRKHLGRKNSSGESGQKTSSTGTASGAPEPGIRHTQDRDSSLSSSNREHKPSIPGSFTTEPESGGQSYGTQFSGTTAAAGESRTSGTADTSRHQRQGLSEPQEISETEGRPHAHTQKQSLPAHYISANEVTGPGAQIIAAPSGGQKQQPGNEQEQLLGSEQHRLLSSHQDRQTGGERGQHLASEHSQHLGSEHNRQLGSEHAQPTHHHQNLGTEHNRSSGRDTSRGHEMGKVESGLTGIPERSHDVGADVPQGEGLNLSRDEKAGYEEYNPSGKGSMDAEARRRSSLAESNDSYNERNRTKSRSMSLGTGPTAGVSKTQSDQSNSPTSPTEGNKEPSTLTGTIKDAWDAVTGKRHTSESEPQQKQAKESTGKRSSDVENEGFSSRQNENNTGIGGKPLTRSGGEYATGVGESHLTHERPEKPSSAMKGSRSTKPEGETHGYSQRKNQGESANKHLGDLEGRKTKENRSSGSHHDDVDPKTRSSETSHPQMETRYERHTTTSPSQSPQEGIQRYEEENVYAKEVVNMPSLVDPSQPTYGSGDRNRTSTSETTSYVTHGSQGKRNVSVGSAGGHEQMTEPSQMPVEKNEHSYEERIPRSERERFDKLKEEHIENRNKNEPHLQPAKAAASAAYPPKTQQGAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5DD47</accession>
    <name>C5DD47_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 12">RNA polymerase II subunit A C-terminal domain phosphatase SSU72</fullName>
            <shortName evidence="12">CTD phosphatase SSU72</shortName>
            <ecNumber evidence="4 12">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0B08250g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the cleavage and polyadenylation factor (CPF) complex, which plays a key role in polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with cleavage factors including the CFIA complex and NAB4/CFIB. SSU72 is required for 3'-end formation of snoRNAs.</text>
    </comment>
    <comment type="function">
        <text evidence="1 12">Processively dephosphorylates Ser-5 of the heptad repeats YSPTSPS in the C-terminal domain of the largest RNA polymerase II subunit (RPB1).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 15">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the cleavage and polyadenylation factor (CPF) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the SSU72 phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="4 12"/>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552146.1">
        <property type="nucleotide sequence ID" value="XM_002552100.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DD47"/>
    <dbReference type="FunCoup" id="C5DD47">
        <property type="interactions" value="1021"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DD47"/>
    <dbReference type="GeneID" id="8290986"/>
    <dbReference type="KEGG" id="lth:KLTH0B08250g"/>
    <dbReference type="eggNOG" id="KOG2424">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062463_0_1_1"/>
    <dbReference type="InParanoid" id="C5DD47"/>
    <dbReference type="OMA" id="TQPNVYQ"/>
    <dbReference type="OrthoDB" id="57957at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008420">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000039">
        <property type="entry name" value="RNA polymerase II subunit A C-terminal domain phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000189">
        <property type="entry name" value="SSU72p Phosphatase and transcription/RNA-processing factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006811">
        <property type="entry name" value="RNA_pol_II_suA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20383">
        <property type="entry name" value="RNA POLYMERASE II SUBUNIT A C-TERMINAL DOMAIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04722">
        <property type="entry name" value="Ssu72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 12" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8 12" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU369031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR21708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="206" mass="23573" checksum="1ECC75472514E377" modified="2009-07-28" version="1">MSNEPTKSLKFCTVCASNNNRSMESHRVLREAGYDVSSYGTGSAVRLPGLSEDKPNVYAFGTPYNDIYNDLRSQSVERYRSNGLLEMLDRNRKLKKAPEKWQEGFKVFDFVFTCEERCFDAVCEDLMNRGGQLNKIVHVININIRDDNENAKIGSKAILKLADMLSQETVRCEKTGEPLEDCIISLLAQWQEMYPQLPLLYSPAYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>C5DD64</accession>
    <name>C5DD64_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0B08646p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0B08646g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552163.1">
        <property type="nucleotide sequence ID" value="XM_002552117.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DD64"/>
    <dbReference type="FunCoup" id="C5DD64">
        <property type="interactions" value="50"/>
    </dbReference>
    <dbReference type="GeneID" id="8291004"/>
    <dbReference type="KEGG" id="lth:KLTH0B08646g"/>
    <dbReference type="eggNOG" id="ENOG502R1XI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_356837_0_0_1"/>
    <dbReference type="InParanoid" id="C5DD64"/>
    <dbReference type="OMA" id="TFWSEDY"/>
    <dbReference type="OrthoDB" id="2155291at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="640"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="663"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="691"/>
            <end position="703"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="737" mass="82890" checksum="B11100109C8F6CD4" modified="2009-07-28" version="1">MTTEASRATFWSEDYTSGIAVLLGNIDNDVSELQEFIEDVETFDNKTVSPAIKSLDSLVRSIPSDTDESLCNKLKQQRKGLLAEILEKLQIANRSNLETACLSQLQALLFEFEEYQQESKELIEKNYGLYSKHLSLAKRARVDVTTRAQRIREASQKPLLRSEETPLSSEEERDYANESSIQFPLTLDAKLSFSSEESFFGFCNKLKKLTKTSKRLIYIPGVSNEYFSSSALFESLKRIEPRMDASLYNLERLGQTLINNEIISPYNGILGAKVSFARDGYYVWGENSSPVIRPAEEKPLRRADVFNGLFKKIGRSSGSSEQIETTEELGLRQRELVDREDHFFQECQNLDYWRTQLELELQKCMNQYHKMLRHKIQILNKASLQFSSLLKEALSTQSSLTPVGADQEIRRIYRANYGIVGHYVPQPGVVFAKHNQLGELTGVAMFHADLQEFPTNELGVAIILHILLEYLWGCDALEVLQAWQLPLDLRRVSRLRRECIVEFKETDGSHSASLANILASKPRPLADVVGLLQSWLLELPDSLIPMICYEEISRTQSIEGMSMAPAEHLVNLAAVCDHFRWLVQRCGGEKVRTAFDSRTDFPLYHVFARTRTRNPQEAPVVSGVVHEILCKDESAERLREMAKNSNARTDARAEARADLQAGSSKHSSPPSTSRLATPAVFVPRPLKAAHSDASSSRPSSKRISGLDLAVVGQSDSESETEPKESKEPSTLAAGVAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>C5DD77</accession>
    <name>C5DD77_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">methionine--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Methionyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">KLTH0B08976g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>tRNA(Met) + L-methionine + ATP = L-methionyl-tRNA(Met) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13481"/>
            <dbReference type="Rhea" id="RHEA-COMP:9667"/>
            <dbReference type="Rhea" id="RHEA-COMP:9698"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78530"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.10" evidence="3"/>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552176.1">
        <property type="nucleotide sequence ID" value="XM_002552130.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DD77"/>
    <dbReference type="FunCoup" id="C5DD77">
        <property type="interactions" value="1128"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DD77"/>
    <dbReference type="GeneID" id="8291019"/>
    <dbReference type="KEGG" id="lth:KLTH0B08976g"/>
    <dbReference type="eggNOG" id="KOG1247">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009710_4_1_1"/>
    <dbReference type="InParanoid" id="C5DD77"/>
    <dbReference type="OMA" id="HLNTTEY"/>
    <dbReference type="OrthoDB" id="5844513at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017102">
        <property type="term" value="C:methionyl glutamyl tRNA synthetase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004825">
        <property type="term" value="F:methionine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006431">
        <property type="term" value="P:methionyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07957">
        <property type="entry name" value="Anticodon_Ia_Met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10290">
        <property type="entry name" value="GST_C_MetRS_N_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00814">
        <property type="entry name" value="MetRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.20:FF:000001">
        <property type="entry name" value="Methionine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000037">
        <property type="entry name" value="Methionyl-tRNA synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.20">
        <property type="entry name" value="Methionyl-tRNA synthetase, Zn-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00098">
        <property type="entry name" value="Met_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041872">
        <property type="entry name" value="Anticodon_Met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018285">
        <property type="entry name" value="Met-tRNA-synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023458">
        <property type="entry name" value="Met-tRNA_ligase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014758">
        <property type="entry name" value="Met-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015413">
        <property type="entry name" value="Methionyl/Leucyl_tRNA_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033911">
        <property type="entry name" value="MetRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029038">
        <property type="entry name" value="MetRS_Zn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00398">
        <property type="entry name" value="metG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001100">
        <property type="entry name" value="PRK00133.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45765">
        <property type="entry name" value="METHIONINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45765:SF1">
        <property type="entry name" value="METHIONINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19303">
        <property type="entry name" value="Anticodon_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09635">
        <property type="entry name" value="MetRS-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09334">
        <property type="entry name" value="tRNA-synt_1g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01041">
        <property type="entry name" value="TRNASYNTHMET"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57770">
        <property type="entry name" value="Methionyl-tRNA synthetase (MetRS), Zn-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methionyl-tRNA synthetase N-terminal heteromerisation" evidence="14">
        <location>
            <begin position="11"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl/Leucyl tRNA synthetase" evidence="13">
        <location>
            <begin position="198"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl-tRNA synthetase anticodon-binding" evidence="15">
        <location>
            <begin position="614"/>
            <end position="750"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF09334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF09635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF19303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAR21738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="751" mass="85357" checksum="292CD1A1478E4B1D" modified="2009-07-28" version="1">MAFNLSFDRSKDHSYALQLANNIKLALAQELAGKEIKISVNDESVKPELTVASTDFRLFNTNAILRYVLNDFEGLESSETHFAVSSLEAMLYHPSAPQEHVKENVSKALENYLQELREPFCATNLITFANAYALSPESLESKFANLPASLSKALSAAKSYTPRLSSSFKRTGAVKINSNFAVKKQDAEILPIEGERNILITSALPYVNNVPHLGNIVGSVLSADIYARYCKARNYNTLFVCGTDEYGTATETKAIEEGCTPRELCDKYHKIHSDVYKWFQIGFDYFGRTTTEKQTEIAQDIFNKLNNNGYLEEHSMKQLYCEEHKAYLADRYVEGECPKCHYEDARGDQCDKCGGLLDPFELINPRCKLDNSKPIPRNSDHIFISLDKLEPRIKEWVEKSSREGGWSKNSRTITQSWLKEGLQPRCITRDLVWGTPVPLEKYKDKVLYVWFDATIGYVSITANYTDGWKKWWKDPENVQLYQFMGKDNVPFHSVIFPGSQLGTNENWTMLHHLSTTEYLQYEGGKFSKSRGVGVFGNNAKDTGVSPSVWRYYLASVRPESSDSHFSWSDFVARNNSELLANLGNFVNRLVKFVNAKYNGVVPEYDQKQLADFVKLEKDINSILSSYVSEMEQSHERRGLEIAMSLSARGNLFLQENKLDNSLFSEFPAKSDAVVGVGLNIVYSVASVIYPFMPEAAETIYKMLNAPAKKIDDKFYLCIEGGHNINKAEYLFKRIDEKEIENWRSKYGGQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDA3</accession>
    <name>C5DDA3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0B09548p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0B09548g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ELP6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552202.1">
        <property type="nucleotide sequence ID" value="XM_002552156.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDA3"/>
    <dbReference type="FunCoup" id="C5DDA3">
        <property type="interactions" value="323"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDA3"/>
    <dbReference type="GeneID" id="8291045"/>
    <dbReference type="KEGG" id="lth:KLTH0B09548g"/>
    <dbReference type="eggNOG" id="ENOG502QSI3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086730_0_0_1"/>
    <dbReference type="InParanoid" id="C5DDA3"/>
    <dbReference type="OMA" id="EYVYHIT"/>
    <dbReference type="OrthoDB" id="9995306at2759"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033588">
        <property type="term" value="C:elongator holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19495">
        <property type="entry name" value="Elp6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018627">
        <property type="entry name" value="ELP6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16184">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16184:SF6">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR21764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="275" mass="30547" checksum="2FDE3B76F005F5F8" modified="2009-07-28" version="1">MSMAQKQDLVIFSDHSVLSRELFSKDLHHVLFVTHKLGTSPSWLINALVETQVLGFPATLNESHAAAVNRASNRKITDSKCATVASFIHNRGFFTSALKKLKVDAECYNIIDYMTDFVLKNYGKPASKVLSSLLEELSNESATSELLILEQPELLVHLLNITSDQLHLELINPLIKKFPLLVVVSYTEAYENVPSDSNEKLSSDMIEQIRFCNSCFFKSAAVLGLKPLATGHASDVSGTLTITRGGASLSHLAVHVVENEYLFLTQKDTTKLFYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDC3</accession>
    <name>C5DDC3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0B10054p</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="9">KLTH0E00462p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0B10054g</name>
        <name evidence="9" type="ordered locus">KLTH0E00462g</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
            <strain evidence="8">CBS 6340</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2009-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Genoscope - CEA"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CBS 6340</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552222.1">
        <property type="nucleotide sequence ID" value="XM_002552176.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553509.1">
        <property type="nucleotide sequence ID" value="XM_002553463.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDC3"/>
    <dbReference type="FunCoup" id="C5DDC3">
        <property type="interactions" value="211"/>
    </dbReference>
    <dbReference type="GeneID" id="8291068"/>
    <dbReference type="GeneID" id="8291645"/>
    <dbReference type="KEGG" id="lth:KLTH0B10054g"/>
    <dbReference type="KEGG" id="lth:KLTH0E00462g"/>
    <dbReference type="eggNOG" id="KOG2533">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_0_1_1"/>
    <dbReference type="InParanoid" id="C5DDC3"/>
    <dbReference type="OrthoDB" id="2985014at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17327">
        <property type="entry name" value="MFS_FEN2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000013">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000018">
        <property type="entry name" value="MFS transporter permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF24">
        <property type="entry name" value="NICOTINIC ACID PLASMA MEMBRANE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="64"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="102"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="155"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="190"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="225"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="295"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="331"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="357"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="386"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="450"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="64"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR21784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR23072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="503" mass="56263" checksum="4912B84F69DD539E" modified="2009-07-28" version="1">MAIGLVKTVQMETTHSVATKDPIKVSIHDDAELSEELAEHVIEEEYYIDPQDESALTRKLDTRLLPMLAFMYFLSALDRSNIGNAYTSGMKEDLRLTSHQYSNAVSVFYSTYLAAELPAVWLLKIAKPRYYMSILVFCWSVITLSSGFVKSYHSLLATRILLGTFEGGFFPAMTLLITMMYKPKEQAKRIAFFFGSSALSGAFGGLIATGLASVNNAGGLEGWRWLYIIEGLISVCASFWLFFGLPDNLDKMKFLNERELSLMQIREKQRIQYMGADPAFSWGQVWLAFKDFKTYFAFVIQFCQDIILYGFSTFLTAILKLGLGYSSREAQYMSVPVYILAAIVFMVSAYVSDRLQVRGPVFFCYNILGATGYIILLAVDNNAVKYFACYLITFSLYTGTGLNITWLTNNVAPHYKRATALGLNQTLGNLSGAIVGQVYTKSPYVFGNSFSLGCIVLSNILTAVQIAWLVKLNKDKAAILAGTKKDTKKERIGDEALDFKYCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDD7</accession>
    <name>C5DDD7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">KLTH0B10450p</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="12">KLTH0D00110p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0B10450g</name>
        <name evidence="12" type="ordered locus">KLTH0D00110g</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
            <strain evidence="11">CBS 6340</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2009-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Genoscope - CEA"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CBS 6340</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552236.1">
        <property type="nucleotide sequence ID" value="XM_002552190.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552733.1">
        <property type="nucleotide sequence ID" value="XM_002552687.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDD7"/>
    <dbReference type="FunCoup" id="C5DDD7">
        <property type="interactions" value="49"/>
    </dbReference>
    <dbReference type="GeneID" id="8291086"/>
    <dbReference type="GeneID" id="8294947"/>
    <dbReference type="KEGG" id="lth:KLTH0B10450g"/>
    <dbReference type="KEGG" id="lth:KLTH0D00110g"/>
    <dbReference type="eggNOG" id="KOG0254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012970_2_1_1"/>
    <dbReference type="InParanoid" id="C5DDD7"/>
    <dbReference type="OrthoDB" id="2241241at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015343">
        <property type="term" value="F:siderophore-iron transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17322">
        <property type="entry name" value="MFS_ARN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000197">
        <property type="entry name" value="Siderophore iron transporter 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF92">
        <property type="entry name" value="GLUTATHIONE EXCHANGER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="165"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="229"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="288"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="320"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="357"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="397"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="424"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="451"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="488"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="561"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="74"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="29"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR21798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR22295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="620" mass="69665" checksum="9BC5531E7BF3BF52" modified="2009-07-28" version="1">MPEYQQGGPSLGSESTPNENYGKANVVQTEKEFSHDSKNEPELGDKLQITKRIVIRKAELMANQYDGWLLKSMFLFSAFICAFSYGLDSIIRSIYMTYAMNDYQTHSLLSTVSVISLTISAVAQIFFAGLSDVFGRLSMFIVAIMFYVVGTVIQSQAYDVQRYAAGSIFYYIGLVGSMFQVTLMLSDCSSLKWRLLYNFVPAWPALVTVWISGNVVHVANPEKNWSWGIAMWAFIFPATCLPIIGCLLHMRWKLRNNSEWKELQEEKTYFQTHGLVQTLKQLFWKLDVVGVLLFSVSTGCILIPLTIAGGASTHWRSAKVIAPLVLGSLLVPFFIYWESKLALVPLAPFKMLKDRGIWAPLLITFLICFIYMMAAGYLYTILLVAGNESSLSATRITSLYSFVAAVFSPVVGIFVARFSRLKPFAVFGCSLYFVTMALFYHFRGGTDTGKGVIGAMVVWGITSCFFNYPINISMQTVTSHENMATVTAFGLTIFRIGGAVGAAVSGAIWTQSLYPRLLKALGDPTLALEAYNMPLSFMLKHEWGTPARDAMIEAYRYVQKYEVLVGLIFVAPMFLLTFFLRDPPLTEDYGQKLEDDEYVKHDDPITDWIAERFSSFKKHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDF3</accession>
    <name>C5DDF3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0C00550p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0C00550g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552252.1">
        <property type="nucleotide sequence ID" value="XM_002552206.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDF3"/>
    <dbReference type="GeneID" id="8291110"/>
    <dbReference type="KEGG" id="lth:KLTH0C00550g"/>
    <dbReference type="HOGENOM" id="CLU_970003_0_0_1"/>
    <dbReference type="InParanoid" id="C5DDF3"/>
    <dbReference type="OrthoDB" id="10462168at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="145"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="153"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="216"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="240"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="266"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="287" mass="31792" checksum="AF004ABD059DDDD2" modified="2009-07-28" version="1">MDDGCLIDFLLEDDFATMNADSSCLDSKWLVEKDCICDDRVIKRPNRKTKSRTKSKRRSLCKELLDESIDELVNLIIKDTKLSLTIGSEPNQRTKVPQTVSSSKKKSRREGWTNKIGDDQGLLTVFGGEQLAGEFAFNLLDVPEPDVTTESNKKKKVKRNRTRNGASKKAESHPIEPTDQSYAATKGHGHSSAPNSNAEKDAAVKKGKNKKKKRKGQADDENKLKGNAKDSTKSNSQPRAESRDRESQRKDKSPINSSSKSGPPVHGKRSSRKKTKSASSSNNDQSC</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-05-03" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDK8</accession>
    <name>MTC6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName>Maintenance of telomere capping protein 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">MTC6</name>
        <name type="ordered locus">KLTH0C01804g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in telomere capping.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MTC6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552307.1">
        <property type="nucleotide sequence ID" value="XM_002552261.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDK8"/>
    <dbReference type="FunCoup" id="C5DDK8">
        <property type="interactions" value="25"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDK8"/>
    <dbReference type="GlyCosmos" id="C5DDK8">
        <property type="glycosylation" value="11 sites, No reported glycans"/>
    </dbReference>
    <dbReference type="GeneID" id="8291166"/>
    <dbReference type="KEGG" id="lth:KLTH0C01804g"/>
    <dbReference type="eggNOG" id="ENOG502QVFP">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033723_0_0_1"/>
    <dbReference type="InParanoid" id="C5DDK8"/>
    <dbReference type="OMA" id="WGTIDPQ"/>
    <dbReference type="OrthoDB" id="5573651at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051008">
        <property type="entry name" value="Telomere_Capping_Maintenance"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057530">
        <property type="entry name" value="TIM-barrel_MTC6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35518:SF2">
        <property type="entry name" value="MAINTENANCE OF TELOMERE CAPPING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35518">
        <property type="entry name" value="MAINTENANCE OF TELOMOERE CAPPING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25506">
        <property type="entry name" value="TIM-barrel_MTC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000407779" description="Maintenance of telomere capping protein 6">
        <location>
            <begin position="20"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="2">
        <location>
            <begin position="20"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="451"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="2">
        <location>
            <begin position="472"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="2">
        <location>
            <position position="43"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="2">
        <location>
            <position position="66"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="2">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="2">
        <location>
            <position position="140"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="2">
        <location>
            <position position="152"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="2">
        <location>
            <position position="227"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="2">
        <location>
            <position position="252"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="2">
        <location>
            <position position="312"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="2">
        <location>
            <position position="320"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="2">
        <location>
            <position position="338"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="2">
        <location>
            <position position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="503" mass="56101" checksum="B41F59AD2FCB8B0E" modified="2009-07-28" version="1" precursor="true">MNRVVYGLLLTLVLNVVRGQSFWPALSSDSLIALRSQRDVLSNISIDKVPMVGVQLNEVAFRGTSNETESLEILASLLNVGVQAYMMDIEFDESYNLTISGSDTSLGTTLSTFKRFISSSNNYLNADMLVLLLRLKQNTNTSTKGAPSNFPNITAILDLYLGSSTLYTPSQLAYDRSSGNVAPSYGNLNSPDWPSLNYFLYSIQKRAVVFYVDTASSDALQSPAIFNASNLNYETSNTPIVCPLTNNAQVLNTSSLSWRFLQKDYSPSDIREYTMCGYSPIIDNKYNPNSINTISNVLENSLLWSWASNEPNTSDDTRSNSTSLVARRCAVVHYTKSNSSSYWTVANCYDRKRALCKRDGNDFEWAVTQEGASYFSHHDQDGNGFCPDNFSLSLPQTALQEKSLDNYLSQLSPEGWEIWIDLNSVSVPDCWVPDGPYASCPYQKEVSTRNFVSMITPVSVFAAVTILMLIMLSWRRVPIQDNRKRWKRVINSHLGSKAEGVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDQ3</accession>
    <name>C5DDQ3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0C02860p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0C02860g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552352.1">
        <property type="nucleotide sequence ID" value="XM_002552306.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDQ3"/>
    <dbReference type="FunCoup" id="C5DDQ3">
        <property type="interactions" value="103"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDQ3"/>
    <dbReference type="GeneID" id="8291213"/>
    <dbReference type="KEGG" id="lth:KLTH0C02860g"/>
    <dbReference type="eggNOG" id="ENOG502S27I">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1619339_0_0_1"/>
    <dbReference type="InParanoid" id="C5DDQ3"/>
    <dbReference type="OMA" id="QYNTVKF"/>
    <dbReference type="OrthoDB" id="18412at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd23024">
        <property type="entry name" value="zf-HIT_ZNHIT2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040722">
        <property type="entry name" value="Hit1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007529">
        <property type="entry name" value="Znf_HIT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18268">
        <property type="entry name" value="Hit1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04438">
        <property type="entry name" value="zf-HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51083">
        <property type="entry name" value="ZF_HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="HIT-type" evidence="3">
        <location>
            <begin position="5"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="33"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="33"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR21914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="155" mass="17506" checksum="39A93A0CC4C837E6" modified="2009-07-28" version="1">MSAKCEICNQEPSKYKCPKCAVRYCSLSCFKDQEKHVHSEKEAEEKKHVSDSEGPEAKSDTGRLPTEQFDELYQNTKEIQELLSYNTVKFHLAKVYRILNIGVGALGSSDTQTSAEVKQQLAVDYLNTLRYGGVHYNEAIEEFCQVSLAKLSNES</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDZ3</accession>
    <name>C5DDZ3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 14">Uroporphyrinogen decarboxylase</fullName>
            <ecNumber evidence="4 14">4.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">KLTH0C04994g</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the sequential decarboxylation of four acetate groups of uroporphyrinogen-III (octacarboxyporphyrin) to yield coproporphyrinogen-III (tetracarboxyporphyrin) with the formation of intermediate hepta-, hexa- and penta-carboxylate porphyrinogens in the heme biosynthesis pathway. Acts on a number of porphyrinogens, but only coproporphyrinogen III can ultimately be converted to heme.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>uroporphyrinogen I + 4 H(+) = coproporphyrinogen I + 4 CO2</text>
            <dbReference type="Rhea" id="RHEA:31239"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:62626"/>
            <dbReference type="ChEBI" id="CHEBI:62631"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 14">
            <text>uroporphyrinogen III + 4 H(+) = coproporphyrinogen III + 4 CO2</text>
            <dbReference type="Rhea" id="RHEA:19865"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57308"/>
            <dbReference type="ChEBI" id="CHEBI:57309"/>
            <dbReference type="EC" id="4.1.1.37"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 4/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the uroporphyrinogen decarboxylase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.37" evidence="4 14"/>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552442.1">
        <property type="nucleotide sequence ID" value="XM_002552396.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDZ3"/>
    <dbReference type="FunCoup" id="C5DDZ3">
        <property type="interactions" value="968"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDZ3"/>
    <dbReference type="GeneID" id="8291309"/>
    <dbReference type="KEGG" id="lth:KLTH0C04994g"/>
    <dbReference type="eggNOG" id="KOG2872">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040933_0_0_1"/>
    <dbReference type="InParanoid" id="C5DDZ3"/>
    <dbReference type="OMA" id="LWLMRQA"/>
    <dbReference type="OrthoDB" id="339900at2759"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00321"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004853">
        <property type="term" value="F:uroporphyrinogen decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00717">
        <property type="entry name" value="URO-D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.210:FF:000004">
        <property type="entry name" value="Uroporphyrinogen decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00218">
        <property type="entry name" value="URO_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038071">
        <property type="entry name" value="UROD/MetE-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006361">
        <property type="entry name" value="Uroporphyrinogen_deCO2ase_HemE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000257">
        <property type="entry name" value="Uroporphyrinogen_deCOase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01464">
        <property type="entry name" value="hemE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21091">
        <property type="entry name" value="METHYLTETRAHYDROFOLATE:HOMOCYSTEINE METHYLTRANSFERASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21091:SF169">
        <property type="entry name" value="UROPORPHYRINOGEN DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01208">
        <property type="entry name" value="URO-D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51726">
        <property type="entry name" value="UROD/MetE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00906">
        <property type="entry name" value="UROD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00907">
        <property type="entry name" value="UROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 14" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="8" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="9 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="10 14" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Uroporphyrinogen decarboxylase (URO-D)" evidence="16">
        <location>
            <begin position="30"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="Uroporphyrinogen decarboxylase (URO-D)" evidence="17">
        <location>
            <begin position="153"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00052550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00058098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS00906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS00907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAR22004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="368" mass="41880" checksum="4F3662E31311C171" modified="2009-07-28" version="1">MQLLDRSKFPELKNDLILRAALGQPVERPPCWIMRQAGRYLPEYHKVKDGRDFFETCRDAEIASEITIQPVRRYAGLLDAAIIFSDILVIPQALGMRVEMLEGKGPHFPELIRTEEDMKKVLDYHTDVLKELDWAFRAITVTRHKLGGQVPLLGFCGGPWTLLVYMTEGGGSRLFRFAKQWLNESPELCHKLLQKITDVAVEFLAQQVVAGAQMLQVFESWGGELGPRDFDEFSLPYIRQISKRLPHRLKELGIEEQIPLTIFSKGSWYALDKLCDSGYNAVSLDWLWDPKDAVKVNAGRVTLQGNLDPGVIYGTDEVISKRVEEMITGFGGGKKQYIVNFGHGTHPFMDPEKIKFFLQECHRVGSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>C5DE32</accession>
    <name>C5DE32_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0C05896p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0C05896g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552481.1">
        <property type="nucleotide sequence ID" value="XM_002552435.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DE32"/>
    <dbReference type="GeneID" id="8291349"/>
    <dbReference type="KEGG" id="lth:KLTH0C05896g"/>
    <dbReference type="HOGENOM" id="CLU_1023326_0_0_1"/>
    <dbReference type="InParanoid" id="C5DE32"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="170"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="272" mass="29395" checksum="4412B69F3F9A5A6A" modified="2009-07-28" version="1">MSCLRFNVLLCDPLALPGSFNVSVPTYLSCYFTGPVLTLRRLRFPVQASNVFPISVRALPGHGLCAGAGARRARASACAYRSAYSPRRRARTARGSQPSTMPDACLGPASSPPTNTLRPARAPRRAVSSSSAATHAKKDPLKPPKSFSTPEKSEHQKPTPTPTHQGHSHHVTEQSHVRRPRPDHVILRIVLCASLAARSHMPVRIRATGPTERGRSWARQEGEGIRPPFDIHASGRAGSASSLKRAIVDSNSVGLLQRLTSVEGRQDPKALH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-05-03" modified="2025-06-18" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>C5DE35</accession>
    <name>MAP2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methionine aminopeptidase 2</fullName>
            <shortName evidence="1">MAP 2</shortName>
            <shortName evidence="1">MetAP 2</shortName>
            <ecNumber evidence="1">3.4.11.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Peptidase M</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">MAP2</name>
        <name type="ordered locus">KLTH0C05962g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.</text>
            <dbReference type="EC" id="3.4.11.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="1">Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe(2+)-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M24A family. Methionine aminopeptidase eukaryotic type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.18" evidence="1"/>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552484.1">
        <property type="nucleotide sequence ID" value="XM_002552438.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DE35"/>
    <dbReference type="SMR" id="C5DE35"/>
    <dbReference type="FunCoup" id="C5DE35">
        <property type="interactions" value="1228"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DE35"/>
    <dbReference type="MEROPS" id="M24.002"/>
    <dbReference type="GeneID" id="8291352"/>
    <dbReference type="KEGG" id="lth:KLTH0C05962g"/>
    <dbReference type="eggNOG" id="KOG2775">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015857_7_1_1"/>
    <dbReference type="InParanoid" id="C5DE35"/>
    <dbReference type="OMA" id="PFAKRWL"/>
    <dbReference type="OrthoDB" id="7848262at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004239">
        <property type="term" value="F:initiator methionyl aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01088">
        <property type="entry name" value="MetAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03175">
        <property type="entry name" value="MetAP_2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050247">
        <property type="entry name" value="Met_Aminopeptidase_Type2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001714">
        <property type="entry name" value="Pept_M24_MAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002468">
        <property type="entry name" value="Pept_M24A_MAP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018349">
        <property type="entry name" value="Pept_M24A_MAP2_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00501">
        <property type="entry name" value="met_pdase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45777">
        <property type="entry name" value="METHIONINE AMINOPEPTIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45777:SF2">
        <property type="entry name" value="METHIONINE AMINOPEPTIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00599">
        <property type="entry name" value="MAPEPTIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01202">
        <property type="entry name" value="MAP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0031">Aminopeptidase</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000407654" description="Methionine aminopeptidase 2">
        <location>
            <begin position="1"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="305"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="401"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="401"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="420" mass="47002" checksum="9F4BB358F38D7178" modified="2009-07-28" version="1">MSDAIAKDAVNTSSEKEPVSATPELKTSGSPDAAVSSGDKKKKKKKKKTNNIKNIALLYPDEKYPEGEWMDYHQDFNLQRTTDEEKRYLTRDAENQQRWNDMRKGAEIHRRVRKNLQNKLKPDMTLTEVVNIVENATRKFTGVDENGDHVDRPKSQGVGFPTGVSLNHCAAHFTPNAGDQTVLRYEDVMKVDIGVQVNGYIVDSAWTVAFDPKYDNLLQAVREATNTGVREAGIDVRLTDIGEAIQEVMESYEVELNGKTHQVKPCRNLCGHNIAPYRIHGGKSVPIVKNGDQTKMEEGEHFAIETFGSTGRGFVVPGGEVSHYAKSAEADGLPAPSLSRAKSLLKTIDENFGTLPFCRRYLDRLGEDKYLFALNSLVKHGIVQDYPPLNDVQGSYTAQFEHTILLHPHRKEVVSRGEDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEE4</accession>
    <name>C5DEE4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Succinate dehydrogenase assembly factor 2, mitochondrial</fullName>
            <shortName evidence="4">SDH assembly factor 2</shortName>
            <shortName evidence="4">SDHAF2</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0C08514g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Plays an essential role in the assembly of succinate dehydrogenase (SDH), an enzyme complex (also referred to as respiratory complex II) that is a component of both the tricarboxylic acid (TCA) cycle and the mitochondrial electron transport chain, and which couples the oxidation of succinate to fumarate with the reduction of ubiquinone (coenzyme Q) to ubiquinol. Required for flavinylation (covalent attachment of FAD) of the flavoprotein subunit of the SDH catalytic dimer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Interacts with the flavoprotein subunit within the SDH catalytic dimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SDHAF2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552593.1">
        <property type="nucleotide sequence ID" value="XM_002552547.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEE4"/>
    <dbReference type="FunCoup" id="C5DEE4">
        <property type="interactions" value="338"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEE4"/>
    <dbReference type="GeneID" id="8291467"/>
    <dbReference type="KEGG" id="lth:KLTH0C08514g"/>
    <dbReference type="eggNOG" id="KOG3326">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103054_0_1_1"/>
    <dbReference type="InParanoid" id="C5DEE4"/>
    <dbReference type="OMA" id="YGKPQNP"/>
    <dbReference type="OrthoDB" id="284292at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006121">
        <property type="term" value="P:mitochondrial electron transport, succinate to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034553">
        <property type="term" value="P:mitochondrial respiratory chain complex II assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.250:FF:000002">
        <property type="entry name" value="Succinate dehydrogenase assembly factor 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.250">
        <property type="entry name" value="Flavinator of succinate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03057">
        <property type="entry name" value="SDHAF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005631">
        <property type="entry name" value="SDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036714">
        <property type="entry name" value="SDH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028882">
        <property type="entry name" value="SDHAF2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12469">
        <property type="entry name" value="PROTEIN EMI5 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12469:SF2">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE ASSEMBLY FACTOR 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03937">
        <property type="entry name" value="Sdh5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109910">
        <property type="entry name" value="YgfY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2 4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR22155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="159" mass="18544" checksum="5BDF73A4480ED644" modified="2009-07-28" version="1">MTMLTRTLYQALRTPALYRKAALPRMAALYSSQGGEPVVISEKDEVRVRLEPIKRAGETLENKRARLVYQSRKRGILETDLLLSRFAAKYLSSMTPEELEEYDALLDELDWDIYYWATKNYAITPLPERFKDSKLLAKLQDFSENKNKEILRMPNLHSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEV9</accession>
    <name>C5DEV9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0D10186p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0D10186g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat G protein beta family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553152.1">
        <property type="nucleotide sequence ID" value="XM_002553106.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEV9"/>
    <dbReference type="FunCoup" id="C5DEV9">
        <property type="interactions" value="501"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEV9"/>
    <dbReference type="GeneID" id="8295391"/>
    <dbReference type="KEGG" id="lth:KLTH0D10186g"/>
    <dbReference type="eggNOG" id="KOG0286">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_57_34_1"/>
    <dbReference type="InParanoid" id="C5DEV9"/>
    <dbReference type="OMA" id="PLDSQWV"/>
    <dbReference type="OrthoDB" id="10255630at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016346">
        <property type="entry name" value="G-protein_beta_1-5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001632">
        <property type="entry name" value="WD40_G-protein_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19850">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN BETA G PROTEIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25391">
        <property type="entry name" value="WD40_Gbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002394">
        <property type="entry name" value="GN-bd_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00319">
        <property type="entry name" value="GPROTEINB"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="81"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="170"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="211"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="385"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR22714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="421" mass="45765" checksum="90E3F0A5B9099E50" modified="2009-07-28" version="1">MIVKNGTSYEHSHHNQALPLAVDVENSVKDQIALARSDTRQLQDLITRVKAKVRDANLAQMSKNVPSLRGGSVNLASHLTLNGHNNKISDFAWSSDSQSILSASQDGFMIIWDAALGFKKNAIPLDSQWVLTCAINASGNLVASAGLTNNCTIYRISQENRIQQQIVSIFKGHTCYVSQVEFFGGNEVITASGDMTCALWDIPKAKRVAEFSDHLGDVLALALPPPKTQPEKTVFASGGSDGYVYIWDTRMRTAGQSFFVSESDVSTLKFFNNGNAILTGTDDGIARMFDLRSDCVIAAYSLSQGLSQRTNSPGKQHSEFSLEYGLSPGKNSFTRATRSANSTYLDNQGIVSLDFSGSGRLIHACYTDYGCVIWDTLKAEIVGKLEGHSSRISGVKTSPDGMAVCTGSWDATLKLWSPEYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEY7</accession>
    <name>C5DEY7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0D10824p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0D10824g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the OSBP family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553180.1">
        <property type="nucleotide sequence ID" value="XM_002553134.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEY7"/>
    <dbReference type="FunCoup" id="C5DEY7">
        <property type="interactions" value="129"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEY7"/>
    <dbReference type="GeneID" id="8295419"/>
    <dbReference type="KEGG" id="lth:KLTH0D10824g"/>
    <dbReference type="eggNOG" id="KOG2210">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012334_0_0_1"/>
    <dbReference type="InParanoid" id="C5DEY7"/>
    <dbReference type="OMA" id="SSYWTEH"/>
    <dbReference type="OrthoDB" id="14833at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008142">
        <property type="term" value="F:oxysterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120015">
        <property type="term" value="F:sterol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030011">
        <property type="term" value="P:maintenance of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.120:FF:000010">
        <property type="entry name" value="Oxysterol-binding protein homolog 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2720">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037239">
        <property type="entry name" value="OSBP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000648">
        <property type="entry name" value="Oxysterol-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018494">
        <property type="entry name" value="Oxysterol-bd_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972:SF184">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN HOMOLOG 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01237">
        <property type="entry name" value="Oxysterol_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144000">
        <property type="entry name" value="Oxysterol-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01013">
        <property type="entry name" value="OSBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR22742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="417" mass="46830" checksum="398ABF239231F9CE" modified="2009-07-28" version="1">MSGYAASSTWTSFLKSVASFNGDLSTLTAPPFILSPTSLSEYSQYWGEHPDLFLGPNFITEPDAEGTSAEQLRMLAVTKWFISTLKSQYCSRNESMGTEKKPLNPFLGELFVGKWENKEHPDFGETVLLSEQVSHHPPVTAYTIINDKNKTSLQGYNQVKASISTASLSVKQFGHALLEYGKFNEQYLITLPPLHIEGMLVASPFVELEGKAYIQSSSGLLCVVEFSGRGYFSGKKNSFKARIFQKPSDASDKEKALYTITGQWSGTSTISKGKSKKGEELFYDASKLRVEHLHVKPIEEQHSLESRKAWKKVADAVKGGDFSQIHQEKSALENAQRDLRKQEEAEGTKWKRRWFVLEDYTAGINDKFTSLASNLKLSTKNVPSGTLVGDKEDKKEHSAAHWRFKRELWDSEKEIKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>C5DF69</accession>
    <name>C5DF69_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 13">Phosphomevalonate kinase</fullName>
            <ecNumber evidence="3 13">2.7.4.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0D12672g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(R)-5-phosphomevalonate + ATP = (R)-5-diphosphomevalonate + ADP</text>
            <dbReference type="Rhea" id="RHEA:16341"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57557"/>
            <dbReference type="ChEBI" id="CHEBI:58146"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:16342"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 13">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the GHMP kinase family. Mevalonate kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.2" evidence="3 13"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553262.1">
        <property type="nucleotide sequence ID" value="XM_002553216.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DF69"/>
    <dbReference type="FunCoup" id="C5DF69">
        <property type="interactions" value="134"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DF69"/>
    <dbReference type="GeneID" id="8295502"/>
    <dbReference type="KEGG" id="lth:KLTH0D12672g"/>
    <dbReference type="eggNOG" id="KOG4519">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022059_1_0_1"/>
    <dbReference type="InParanoid" id="C5DF69"/>
    <dbReference type="OMA" id="LVIHRTM"/>
    <dbReference type="OrthoDB" id="10262935at2759"/>
    <dbReference type="UniPathway" id="UPA00057">
        <property type="reaction ID" value="UER00099"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004631">
        <property type="term" value="F:phosphomevalonate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010142">
        <property type="term" value="P:farnesyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019287">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000101">
        <property type="entry name" value="Phosphomevalonate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016005">
        <property type="entry name" value="Erg8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035102">
        <property type="entry name" value="Phosphomevalonate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01219">
        <property type="entry name" value="Pmev_kin_ERG8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31814">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31814:SF2">
        <property type="entry name" value="PHOSPHOMEVALONATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017288">
        <property type="entry name" value="PMK_GHMP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 13" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="10 13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 13" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="11 13" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="5 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="14">
        <location>
            <begin position="141"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR017288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR22824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="445" mass="49261" checksum="44CAB2ECE7C07977" modified="2009-07-28" version="1">MVNRAFSAPGKALLVGGYLVLDPQYRSYVVALSSRMHAVVSQAAPCDEKSFKVTIKSSQFNSDEWNYMIAGEAPFEVRELDGRKNPFAEKVVEVIMNYCQPSPTETGDILVEVYSDPGYHSVGTSMLKRNSYKEFRFHDETITKVPKTGLGSSAGLVTVLVSALLSIFKPSLNVRAEKDLVTIHNLSQVAHCQAQGKVGSGFDVAAAVFGSIMYQRFDPELINNLPAANSIHYAQALRKLVDETDWKFEHENITLPPGFRLIMGDVNNGSETTKLVAKVKAWYDLNYPRSLDIYQKINEGNVSFIEGIGKLASFSKESPSEYNCMLQSLNSGADFETYDIIKQIRQAVDQVRAKFRLITDESGADIEPPVQTQLLDQSLNLKGVLTGMVPGAGGFDAISLIVTDNSDIPSQTRNDGKFSSVTWLNLRQQNTGVKEEIPEHYDKFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFA3</accession>
    <name>C5DFA3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0D13486p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0D13486g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553296.1">
        <property type="nucleotide sequence ID" value="XM_002553250.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFA3"/>
    <dbReference type="FunCoup" id="C5DFA3">
        <property type="interactions" value="75"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFA3"/>
    <dbReference type="GeneID" id="8295538"/>
    <dbReference type="KEGG" id="lth:KLTH0D13486g"/>
    <dbReference type="eggNOG" id="KOG3780">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018982_3_0_1"/>
    <dbReference type="InParanoid" id="C5DFA3"/>
    <dbReference type="OMA" id="KGNYNLP"/>
    <dbReference type="OrthoDB" id="2333384at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070086">
        <property type="term" value="P:ubiquitin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050357">
        <property type="entry name" value="Arrestin_domain-protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188">
        <property type="entry name" value="ARRESTIN DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188:SF62">
        <property type="entry name" value="ARRESTIN-RELATED TRAFFICKING ADAPTER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01017">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="2">
        <location>
            <begin position="259"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="561"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="609"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="561"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="618"/>
            <end position="630"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR22858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="653" mass="71848" checksum="36ECDD84E4276FB1" modified="2009-07-28" version="1">MSMFSLGKLSSSSTQPIYFDVRLKSSYKNIVLIQGTPLEASPIPISGHIVFSVPETISVKKIALKLAGTFKLEFLQVGHHKNSSVASIVKERRTIFECVWDNLLVSPIGRITVGDPSHCESQPELPSGATPRPHLIQSLSTPTINNLWKPKKSNSAIGLDKSSDKSFTSLFDGISTGPGHTFVIQKGNYELPFKVSLPPNIAETIEGLQAGSVLYKFESQMDRGGFKNAFTKHKYVRIFRTLSPNNLAVGEEMSVGKSWPDRLQYEISIPSRAIPVGGVTPVTVSLYPFQKGYKLYKIEVSLIQYYAFKDQNEQMYDDENIVFHKVMKDFKDLGGCDGSVDNTIVDKVVLNSSVHLPANLKQVTQDCDIGGDAIRVRHKLSIKITLKKRVGEEDKKTEIKANIPVFLYISPHVPVEGRLVLLDNTGKVHFRSGEGVPLFEKQILNTTLASSTSLNNLQRSDDGSLSVNYFPEEDTEAPPTYAKHVYDQVFDADNDISQLEALRSTSTTPLAKCTLRAPLDSQIRSLSLENLNRVPSYEQVVDDVEDEPALPICELTPSYEVSNPLSQPCSTPNSAENSRPTSPEPANIASRKSQGTLHGGLAHFVRAQIPDRAKSPVHSRQAQDSSSHTAGSHLHNLLGHSKSGDKVPSSHKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFA8</accession>
    <name>C5DFA8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Elongation of fatty acids protein</fullName>
            <ecNumber evidence="10">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0D13596g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>an acyl-CoA + malonyl-CoA + H(+) = a 3-oxoacyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:50252"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:90726"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:50253"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the ELO family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="10"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553301.1">
        <property type="nucleotide sequence ID" value="XM_002553255.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFA8"/>
    <dbReference type="FunCoup" id="C5DFA8">
        <property type="interactions" value="505"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFA8"/>
    <dbReference type="GeneID" id="8295543"/>
    <dbReference type="KEGG" id="lth:KLTH0D13596g"/>
    <dbReference type="eggNOG" id="KOG3071">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048483_6_1_1"/>
    <dbReference type="InParanoid" id="C5DFA8"/>
    <dbReference type="OMA" id="CRFPMGW"/>
    <dbReference type="OrthoDB" id="434092at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009922">
        <property type="term" value="F:fatty acid elongase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034625">
        <property type="term" value="P:fatty acid elongation, monounsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034626">
        <property type="term" value="P:fatty acid elongation, polyunsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019367">
        <property type="term" value="P:fatty acid elongation, saturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030457">
        <property type="entry name" value="ELO_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002076">
        <property type="entry name" value="ELO_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157:SF157">
        <property type="entry name" value="ELONGATION OF FATTY ACIDS PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157">
        <property type="entry name" value="FATTY ACID ACYL TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01151">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01188">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5 10" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 10" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="71"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="104"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="179"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="206"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="246"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="283"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="327"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="335"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR22863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="346" mass="39498" checksum="09D27ABE09D395B9" modified="2009-07-28" version="1">MEQVQTQAQSFAAELLQKVESVPWISVHTPSIEHPFGLELWPIFSKVFEKYAGYPAEDFQFIHNKTFMANGYQAVGVIIVYYIVIFGGQALLRAANAPPLKLGFLFQLHNLVLTSVSLVLLLLMVEQLVPMVWSQGLLWSICHKNAFAPKLVTLYYLNYLTKYLELIDTVFLILKRKKLLFLHTYHHGATALLCYTQLTGRTSVEWVPITLNLGVHVIMYWYYFLSARGIRVWWKEWVTRFQIIQFLIDLVFVYFATYTFYAYKYFNGILPNKGTCYGTQAAAAYGYLILTSYLVLFISFYIQSYRKGASKKRAATNAAAKIATEATTAATTGKQSHATKATSRKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFH6</accession>
    <name>C5DFH6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0D15158p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0D15158g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553369.1">
        <property type="nucleotide sequence ID" value="XM_002553323.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFH6"/>
    <dbReference type="FunCoup" id="C5DFH6">
        <property type="interactions" value="187"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFH6"/>
    <dbReference type="GeneID" id="8295613"/>
    <dbReference type="KEGG" id="lth:KLTH0D15158g"/>
    <dbReference type="eggNOG" id="ENOG502RZK7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128326_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFH6"/>
    <dbReference type="OMA" id="FDCHLIP"/>
    <dbReference type="OrthoDB" id="25408at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019488">
        <property type="entry name" value="Nucl_pore_RNA_shuttling_Mtr2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10429">
        <property type="entry name" value="Mtr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="103"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="171" mass="18327" checksum="C54070F71601679F" modified="2009-07-28" version="1">MNGQSQAAENFVKRIFTFLDETDSQQLAPLVQWFQPAGCKIIVNASAFPQPAGFLETWQRCVVQTQHVLTGVDFHVIPGTGSAVCSVNGKVRFDESGRDKAGQDAAVPAPAGAGVGPTGPAGKHRPLWGPYFGLSLQLVVDERALGGDFNGVISSFNYTMVYRPEDSLMVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFH8</accession>
    <name>C5DFH8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0D15202p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0D15202g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553371.1">
        <property type="nucleotide sequence ID" value="XM_002553325.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFH8"/>
    <dbReference type="FunCoup" id="C5DFH8">
        <property type="interactions" value="48"/>
    </dbReference>
    <dbReference type="GeneID" id="8295615"/>
    <dbReference type="KEGG" id="lth:KLTH0D15202g"/>
    <dbReference type="eggNOG" id="ENOG502QVQ9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_402815_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFH8"/>
    <dbReference type="OMA" id="DFKPYMD"/>
    <dbReference type="OrthoDB" id="4068213at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051285">
        <property type="term" value="C:cell cortex of cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009571">
        <property type="entry name" value="SUR7/Rim9-like_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052413">
        <property type="entry name" value="SUR7_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28019">
        <property type="entry name" value="CELL MEMBRANE PROTEIN YLR413W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28019:SF2">
        <property type="entry name" value="CELL MEMBRANE PROTEIN YLR413W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06687">
        <property type="entry name" value="SUR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002947818" evidence="3">
        <location>
            <begin position="28"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="545"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="588"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="631"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="661"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="679"/>
            <end position="693"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR22933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="693" mass="72618" checksum="25924E9676CFAA5E" modified="2009-07-28" version="1">MLKRVPLAVGVLFTLTAFILTIVATAGSNSNHKPINNVYLGEADISRINVTKVIPETGPVLGVLAGLMNGAASGSASGNASAQEAQIEAAANTVIEALKRVAQSPAALQPLMTLLGDSSNVSQTVGALAQVAKSNMSLASSSGSSQQITMVTQLLQTSSNASETLQGLAGMLQTANATSAAQQRAVLGVLGDSSNATASVDALVQLDNQTAASRAQLSPVLAIFQYSSNTTGTLSALGSIMSANISSSTAETLLSALKDSGSSMQQTLSMLAGATSDDSQRASILALELLFNSSSDSQQTLSTLTSLIQSNVTTSPTARNSFAALSSLFSHSSNKTLVLSSVSLLANSSSTASSAQLTALQELLESSTNTNTTLSVLQQVAASSNSSSAAASAQSLTPIASLLAGAKNSTLTLQSALQLTQAMAANSTLFQPLLSILSTAKMGSTDITRDTLDTMMPVIMDNLNVNSHYRLAIFTLCRGLSDGKMRKCNSPHAVQNFVLRDILYDELENSDFKPYMQALNVQKSDMYLEGSLQKKQDSYVPAVRAVLAFNLLAIILSFFLMLAMLAVFFMKSGAGKREKLTLLTSRVLAFLVTLFTLLSGAITAAFVGIIKRDTKKDDYNVSFATGSAFQGLIWTAFVLALITFVLLFFVRPKAEQKDLDLGEFQPAAPTSSSDDPEPLTEKSTKDAPEQHVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFI0</accession>
    <name>C5DFI0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">KLTH0D15246p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0D15246g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. ABCD family. Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553373.1">
        <property type="nucleotide sequence ID" value="XM_002553327.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFI0"/>
    <dbReference type="FunCoup" id="C5DFI0">
        <property type="interactions" value="333"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFI0"/>
    <dbReference type="GeneID" id="8295617"/>
    <dbReference type="KEGG" id="lth:KLTH0D15246g"/>
    <dbReference type="eggNOG" id="KOG0064">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007587_5_0_1"/>
    <dbReference type="InParanoid" id="C5DFI0"/>
    <dbReference type="OMA" id="CHRTSLW"/>
    <dbReference type="OrthoDB" id="422637at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005324">
        <property type="term" value="F:long-chain fatty acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015910">
        <property type="term" value="P:long-chain fatty acid import into peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042760">
        <property type="term" value="P:very long-chain fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03223">
        <property type="entry name" value="ABCD_peroxisomal_ALDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050835">
        <property type="entry name" value="ABC_transporter_sub-D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384">
        <property type="entry name" value="ATP-BINDING CASSETTE, SUB-FAMILY D MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384:SF69">
        <property type="entry name" value="PEROXISOMAL LONG-CHAIN FATTY ACID IMPORT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06472">
        <property type="entry name" value="ABC_membrane_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="119"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="480"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="33"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="34"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="79"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR22935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="798" mass="91123" checksum="B401A7C864AE71D9" modified="2009-07-28" version="1">MLQFYKRHRLRILKTTYVLLLLTMFKSMQARPRIARDGDRLGQQRDKPPRAKPGSLLEITTESETDTESEPSIRAQSSRSDRDDENATERDAGSAGSEKSSPTRHNFMFMLIIKNRKCMALFLFQAILLVMRTVLSLRVATLDGLLVSRLVKAEYRKFLQVLLGQWMVLGIPASIINSLIHYTTRLCAVSVNRVISKHLLDRYMSSHHTFYSVNSIPHGGKNLVAYNASAAVSEDTEELARSDLSVQYLTRDVGAFSYNLSVLLNQLLKPTLDLILCSFKLVSSANNSMMGEGTLALGIIVHVSNMLLKMIQPNFTKLSIQRTHLEGVFRSLHSKIHSSNEEIALLKGQSTELWNLDYKFYQLAIFLNRELRLHSVYDFATTFIIKYTWGAAGLALCSIPIFFQKEHSNQDITAEFITNRRLLLTASSSVGRFVQLKKSIQQLRGVSMRLRQFDQRLDLPPEFAELEQGDSLIDYNDSKIQFINIPLVTPTHQILIPELNFELNHGDHLLIIGPNGCGKSSLFRVLGGLWPIMKSFTHPERESKLIMPPRIGPNGEKPIFYLPQKPFMGNKFTFREQLIYPDTMEKYDARYEGDFSKGDHDLTEILHVLELDDLLTENMSIIMANSTEDSNTIPEVTNQQAFDVIRNWSEELSIGVQQRLAMARMYYHKPRFAVLDECTSAVSPQMEQKMYSHAQGLGISLISVCHRTTLWHFHNYLLKFDGKGDCEFGKFDPEQRLHDEDKLNELNTKLEKDVPLWNRRLQELIIAKKSNVLRKSQTNLNSLSSSKNLVLNSTPANR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFK9</accession>
    <name>C5DFK9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0D15972p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0D15972g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TSR2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553402.1">
        <property type="nucleotide sequence ID" value="XM_002553356.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFK9"/>
    <dbReference type="FunCoup" id="C5DFK9">
        <property type="interactions" value="208"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFK9"/>
    <dbReference type="GeneID" id="8295649"/>
    <dbReference type="KEGG" id="lth:KLTH0D15972g"/>
    <dbReference type="eggNOG" id="KOG4032">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074896_0_1_1"/>
    <dbReference type="InParanoid" id="C5DFK9"/>
    <dbReference type="OMA" id="QSNWGGP"/>
    <dbReference type="OrthoDB" id="263560at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019398">
        <property type="entry name" value="Pre-rRNA_process_TSR2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21250">
        <property type="entry name" value="PRE-RRNA-PROCESSING PROTEIN TSR2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10273">
        <property type="entry name" value="WGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="142"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="149"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR22964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="209" mass="23501" checksum="C8762107F12F6D37" modified="2009-07-28" version="1">MSSNIDEAAYVEAGNGQACLAFKDEKQEARFELGVSMMVYKWDALSIAVENGWGGPESAEKRDWVTAVIVDLFKSEKIVDVTLIEETLLYAMADEFDTNVEDDSALPIAAGIIEIYKQCDAQDFSTVEKLYLDWTEKQKSKGSKRIVQVEEDPLNPDASDSEEEEEEVDGEFEDDDSMAVDEVPEINTEPVVDEDGFELVQKKGRRRHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFQ2</accession>
    <name>C5DFQ2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0D16984p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0D16984g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR23007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553445.1">
        <property type="nucleotide sequence ID" value="XM_002553399.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFQ2"/>
    <dbReference type="GeneID" id="8295694"/>
    <dbReference type="KEGG" id="lth:KLTH0D16984g"/>
    <dbReference type="eggNOG" id="ENOG502RTN8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048223_1_0_1"/>
    <dbReference type="InParanoid" id="C5DFQ2"/>
    <dbReference type="OMA" id="YHRSDEN"/>
    <dbReference type="OrthoDB" id="4689212at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11307">
        <property type="entry name" value="M35_Asp_f2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029482">
        <property type="entry name" value="HRXXH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039124">
        <property type="entry name" value="PRA1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39399">
        <property type="entry name" value="PROTEIN ZPS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39399:SF1">
        <property type="entry name" value="PROTEIN ZPS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13933">
        <property type="entry name" value="HRXXH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="Putative peptidase" evidence="2">
        <location>
            <begin position="20"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR23007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="261" mass="28870" checksum="1B2719EF6810ACF5" modified="2009-07-28" version="1">MKPTAYRARHGIALSATFIVVAILVISMVFGILADRKFNEKSGQTPNNPTVAATAWDDPVLPELYKTCNGTHLETLKSAFEETWNVTSFARSRLLAYGTNDRIYQRWFGDGSLYTVLGTIEALAEVPKKDVLFRCDDVDGLCAANPSYYAGHHRLNATQETVICDYFYTAKKPVSEICSNGTLAEVAPSKYMGIDMVHRYFHVPTVNLDGYIGEHTEEVDEILSMASKNSSFAVRNVDSYLYYLADVYSSAIVPGGCLGDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFT5</accession>
    <name>C5DFT5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0D17776p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0D17776g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR23040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553478.1">
        <property type="nucleotide sequence ID" value="XM_002553432.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFT5"/>
    <dbReference type="FunCoup" id="C5DFT5">
        <property type="interactions" value="26"/>
    </dbReference>
    <dbReference type="GeneID" id="8295729"/>
    <dbReference type="KEGG" id="lth:KLTH0D17776g"/>
    <dbReference type="HOGENOM" id="CLU_959993_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFT5"/>
    <dbReference type="OMA" id="HTCHCAR"/>
    <dbReference type="OrthoDB" id="4035759at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="187"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR23040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="290" mass="32428" checksum="D3F74F4F986F7E01" modified="2009-07-28" version="1">MHNRSILQNSLLFGAPVALIAGILSQFFMPSAEWLLAHGQAGEFSPAVPTTVELENVVHTCHCARQVLSWHDVLVFLKNELLRVNPQVALLAFNLMLILLQMLSSRKMPRAHDEDNAEADLEGEDDETSKFMSFVPKYDSRERALLQFQKLKSKPLNFKYGSDDSYSVRDSSDDAVSLERLLRKFSRRGRSSEVNSTKLKKSQPPETPSSAQTSTIDLSIAVMTPQLTNSVGRSHCTSRTSSFSKRELKNCLVGTPESQSMSFHQPSPAKSSVLSTKVTQEQVYSQPFIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFX6</accession>
    <name>C5DFX6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0D00748p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0D00748g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552756.1">
        <property type="nucleotide sequence ID" value="XM_002552710.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFX6"/>
    <dbReference type="FunCoup" id="C5DFX6">
        <property type="interactions" value="36"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFX6"/>
    <dbReference type="GeneID" id="8294976"/>
    <dbReference type="KEGG" id="lth:KLTH0D00748g"/>
    <dbReference type="eggNOG" id="ENOG502QV98">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015198_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFX6"/>
    <dbReference type="OMA" id="GHRMESI"/>
    <dbReference type="OrthoDB" id="10251809at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011043">
        <property type="entry name" value="Gal_Oxase/kelch_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23244:SF471">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN SUBUNIT BETA 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23244">
        <property type="entry name" value="KELCH REPEAT DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50965">
        <property type="entry name" value="Galactose oxidase, central domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="431"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="445"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="820" mass="91005" checksum="14FC81439B71CBA2" modified="2009-07-28" version="1">MQSGVSSFSGSSDNSQLSLNLQNNAPVAGNYGVPVWTKDHYKSQYKAPPYLSNYVPGLRDASAQVSAERRKLDNEKALIAYYSSKIHVSRHDSIISGCSNPSVGSDNSRRSARMKHALKSTHKTFGDHGSPLRCAHDESKYSPEVFSKGYLNYCSKLPLPVLESTKLLKRHNMWIPMTRWDIRDKNSESEGPSLSHHDGNKEEDSRLFTDVKIHPADYPSNQNTFVGSMTIPPLFGEMKLPPFAYQCTVDLDDNIYTLGGLTSSYLYSDEAPDLSSFHVDGVPNLPPPLLDNVVNNPCMVNNHDLYVISSSSSRVQKPTTTGQVPPPLLCMTGSVLTKRHIFYYGGFEIKTETTVDEQTGKFFLKKRAYLHNTAYILDVVTFKFTKVELVAQPTKFNAYPATVPRFGHAQVSVQLSPTTTLKCAACSASSSSESKTDESKPENIGASSPISQPLDLRSQSETSLTAEKVINTKLSNSLNSGVFTVIVMGGYRQINEDEYETLNDLWKLEVTVTARGKRNYFKFADTALATPFARVLNEHEGGTWPGARAFQACEVYDTELLKTYSSKPKLLENLRANFCIEPETLSPGVMSPGEKAPSLTRRSELYENHHIHAYGDSTCSGAWNHKSNANTQFTGKTLLVHGGSDKEHVYGDMWWFDLDNEEWTLVPTYVKRTDSKCHSNEVQLPLVGHTLVRIASNAVLTGGLSQEDVDKLWSRNKTQSDPNPALPPSLTGALHLLDLSSLVLKPFLLGTDPNEIQRARNIGWPEVLRFICAPGMHKNGFVYLVGGMMFNISKVEELYLRGTMTVFILPIITTPKVVTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>C5DG34</accession>
    <name>C5DG34_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">KLTH0D02068p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0D02068g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552814.1">
        <property type="nucleotide sequence ID" value="XM_002552768.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DG34">
        <property type="interactions" value="708"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DG34"/>
    <dbReference type="GeneID" id="8295034"/>
    <dbReference type="KEGG" id="lth:KLTH0D02068g"/>
    <dbReference type="eggNOG" id="KOG0160">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000192_9_0_1"/>
    <dbReference type="InParanoid" id="C5DG34"/>
    <dbReference type="OMA" id="EIMFDDR"/>
    <dbReference type="OrthoDB" id="6108017at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15480">
        <property type="entry name" value="fMyo2p_CBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01380">
        <property type="entry name" value="MYSc_Myo5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.190">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002710">
        <property type="entry name" value="Dilute_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046943">
        <property type="entry name" value="Fungal_Myo2/2A_CBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036103">
        <property type="entry name" value="MYSc_Myo5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004009">
        <property type="entry name" value="SH3_Myosin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF706">
        <property type="entry name" value="DILUTE CLASS UNCONVENTIONAL MYOSIN, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01843">
        <property type="entry name" value="DIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM01132">
        <property type="entry name" value="DIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50084">
        <property type="entry name" value="Myosin S1 fragment, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51126">
        <property type="entry name" value="DILUTE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51844">
        <property type="entry name" value="SH3_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="4 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 9" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6 9" id="KW-0518">Myosin</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Myosin N-terminal SH3-like" evidence="13">
        <location>
            <begin position="4"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="Myosin motor" evidence="12">
        <location>
            <begin position="71"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="domain" description="Dilute" evidence="11">
        <location>
            <begin position="1207"/>
            <end position="1482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="9">
        <location>
            <begin position="647"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="981"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="982"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="165"/>
            <end position="172"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR22376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1556" mass="178068" checksum="1E0530CEA6E4E12A" modified="2009-07-28" version="1">MSFEVGTRCWYPDKNEGWVGSEVTKLSQPADGKYLLELTLENGESVEIETNTIAEGQDDRLPLLRNPPILEAIEDLTSLSYLNEPAVLHAIKARYGQLNIYTYSGIVLIATNPFDRVEQLYSQDMIQAYAGRRKGELEPHLFAIAEEAYRLMKNDKQNQTIVVSGESGAGKTVSAKYIMRYFASVEQANNDNTSSDHQLEMSETEQRILATNPIMEAFGNAKTTRNDNSSRFGKYLEILFDQNTSIIGARIRTYLLERSRLVFQPQTERNYHIFYQLLEGLSESEKAELHLTKVEDYHYLNQGGDYRIKGVDDAAEYRTTIDALKMVGFAQDTQHQLFKILAALLHIGSIEVKKTRNDSSLSSDEPNLQIACDLLGIDAYGFSKWITKKQITTRSEKIVSNLSFNQAIVARDSVAKFIFSALFDWLVENINTVLCNPEVSNQVSSFIGVLDIYGFEHFEKNSFEQFCINYANEKLQQEFNQHVFKLEQEEYMNEQIEWSFIEFNDNQPCIDLIENKLGILSLLDEESRLPAGSDESWTQKLYQTLDKPPTNSVFSKPRFGQTKFVVSHYALDVAYDVDGFIEKNRDTVSDGHLEVLKASKNETLLAILDTIDNNAAALAKKQEANKKPGPARMVKKPTLGSMFKQSLIDLMTTIDSTNVHYIRCIKPNEDKEAWKFDNLMVLSQLRACGVLETIRISCAGFPSRWTYNEFVLRYHILIPSENWTKIFTSEATEDDIRDLCKQILDVTVKDSTKYQLGNTKIFFKAGMLAYLEKLRGTKMHNACVMIQKKIKGVYYRNKYLAIQSAIHKSQAAVAGALCRQRVDYEIKTLAATSLQSLLRAHTQRKHLKNTFCSIIRVQSLVRRRITQKELLERREFDAAIAIQKKIRGFVPRKHFNTTRGSSVRIQSLVRRKLAQKQLKQLKADAKSVNHLQEVSYKLENKVIQLTQSLADKVKENREMTSRIEELQKSLSESANIKTLLESQKEEHSRDLQEQKNSHDAELANKRAELEQAKEEIAAAKQEIDSLMTKQEELRNDVRLKIENLNKAQQEYADAQTQNSDLKNEVKSLKDEISRLQATIRSGVSANTALAHTPTKNRRFSTHSSLADGMSPRQLNVISMNNGMEDDVRSTASALSQINDELYKILEDTKSLNTEIVEGLLKGFKIPETGVAAELTRKEVLYPARIMIIVLSDMWRLGLTKQSESFLAEAMSTIQKLVTGLKGEDMICHGAFWLTNVRELYSFVVFAQESILNDDSYNSGLNEDEYKEYVTLVTELKDDFESLSYNIYNIWLKKLQKDLEKKAVSAVVMSQSLPGFIANESSQFLPKLFTQSNYYKMDDILTFFNNIYWSMKTYHVEQEVFREVIITLLKYVDSICFNDLIMRRNFLSWKRGLQLNYNVTRLEEWCKSHHIPEGTDCLQHMLQASKLLQLKKANLDDIDIIWEICSSLKPAQIQKLITQYAVADYEVPIPQEILTFVANRVKSESSLSSNGKSQTHSSDIFLHVENGPFEDPFSLIETRRFGKIEAYIPAWMNLPLTRRVVELVTQHVTVQEVERAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>C5DG36</accession>
    <name>C5DG36_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0D02112p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0D02112g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552816.1">
        <property type="nucleotide sequence ID" value="XM_002552770.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DG36"/>
    <dbReference type="FunCoup" id="C5DG36">
        <property type="interactions" value="75"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DG36"/>
    <dbReference type="GeneID" id="8295036"/>
    <dbReference type="KEGG" id="lth:KLTH0D02112g"/>
    <dbReference type="eggNOG" id="ENOG502S0N2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091781_0_0_1"/>
    <dbReference type="InParanoid" id="C5DG36"/>
    <dbReference type="OMA" id="HKWELDI"/>
    <dbReference type="OrthoDB" id="4034365at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070336">
        <property type="term" value="F:flap-structured DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000736">
        <property type="term" value="P:double-strand break repair via single-strand annealing, removal of nonhomologous ends"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021624">
        <property type="entry name" value="Saw1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11561">
        <property type="entry name" value="Saw1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR22378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="283" mass="32007" checksum="F5509A9C72F76565" modified="2009-07-28" version="1">MVQVQSGVVLPIRIFINRQQVAKTIQDSSTSFETPLLPNNSIIRLKSPLIRVYLSNTDARDLCNDIKKEILLIIYELTSKDVHDTVTGKLKIGNCVDIKEAFGKTRAFSFLYDSDPDRCNLTTLERIGKHQYKLHYDKNWELDIFITDLRKLARIRSVLLARMSWQGSVFPGAAIPVGDLSGTRILRRCREEIMSKKSPTDEPSILLESDDEGALLDTAAVDSSTSTTTRPLKEADLDEDKKPAFKFIHSPMVNLGNCIDIHVLKRPKRARTNRSQAHLDVET</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGK7</accession>
    <name>C5DGK7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">KLTH0D06116p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0D06116g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sphingosine N-acyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552987.1">
        <property type="nucleotide sequence ID" value="XM_002552941.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGK7"/>
    <dbReference type="FunCoup" id="C5DGK7">
        <property type="interactions" value="487"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGK7"/>
    <dbReference type="GeneID" id="8295217"/>
    <dbReference type="KEGG" id="lth:KLTH0D06116g"/>
    <dbReference type="eggNOG" id="KOG1607">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028277_4_0_1"/>
    <dbReference type="InParanoid" id="C5DGK7"/>
    <dbReference type="OMA" id="LCRLCML"/>
    <dbReference type="OrthoDB" id="3053196at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050291">
        <property type="term" value="F:sphingosine N-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016439">
        <property type="entry name" value="Lag1/Lac1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006634">
        <property type="entry name" value="TLC-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12560:SF11">
        <property type="entry name" value="CERAMIDE SYNTHASE LAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12560">
        <property type="entry name" value="LONGEVITY ASSURANCE FACTOR 1 LAG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03798">
        <property type="entry name" value="TRAM_LAG1_CLN8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00724">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50922">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="78"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="123"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="174"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="216"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="246"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="294"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="345"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="TLC" evidence="12">
        <location>
            <begin position="162"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="383"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="396"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR22549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="421" mass="48755" checksum="9BD02C0822688F4F" modified="2009-07-28" version="1">MSSHLQKPVVRTRRTSSVGAINLGDNKVPGLSTMKESEQSKTAALARMRALSEASKGDVDLLKKFYLTYREMSYRHTWLTPLVILLAVYGAYFLSGNRTASNPLHPFVAISYQVGDSDMYGKGIGDLCFVFYYMVFFTFLREFLMECVLRPLASALGVTGEHKTKRLMEQAYSIIYYGISGPFGLYIMYHTDLWLFRTAPMYATYPDLTNQYLYKVFYLGQAAFWTQQACVLVLQLEKPRKDFKELVFHHIVTLALIWLSYVFHFTKMGLAVYITMDVSDFFLSLSKTLNYLDSALTAPVFFVFVASWTYLRHYINLKILWSVLTEFRTVGDYTLNFATQQYKCWISLPIVFVLISALQLVNLYWLFLVMRILYRLLFKGIQKDERSESESEETVQVDKHENDENEKTMEGNPDPALKKEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGL9</accession>
    <name>C5DGL9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0D06402p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0D06402g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) = a 1,2-diacyl-sn-glycero-3-phospho-L-serine(out)</text>
            <dbReference type="Rhea" id="RHEA:38663"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:38664"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the OSBP family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552999.1">
        <property type="nucleotide sequence ID" value="XM_002552953.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGL9"/>
    <dbReference type="FunCoup" id="C5DGL9">
        <property type="interactions" value="1385"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGL9"/>
    <dbReference type="GeneID" id="8295230"/>
    <dbReference type="KEGG" id="lth:KLTH0D06402g"/>
    <dbReference type="eggNOG" id="KOG2210">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012334_4_2_1"/>
    <dbReference type="InParanoid" id="C5DGL9"/>
    <dbReference type="OMA" id="VHTHKKD"/>
    <dbReference type="OrthoDB" id="14833at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070273">
        <property type="term" value="F:phosphatidylinositol-4-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005548">
        <property type="term" value="F:phospholipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120015">
        <property type="term" value="F:sterol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030011">
        <property type="term" value="P:maintenance of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016125">
        <property type="term" value="P:sterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.120:FF:000007">
        <property type="entry name" value="Oxysterol binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.2720:FF:000001">
        <property type="entry name" value="Oxysterol-binding OBPalpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2720">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037239">
        <property type="entry name" value="OSBP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000648">
        <property type="entry name" value="Oxysterol-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018494">
        <property type="entry name" value="Oxysterol-bd_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972:SF102">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01237">
        <property type="entry name" value="Oxysterol_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144000">
        <property type="entry name" value="Oxysterol-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01013">
        <property type="entry name" value="OSBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="4" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00050362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR22561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="433" mass="49084" checksum="FAD688EAF7E80481" modified="2009-07-28" version="1">MPLNKLKTRSAAVPGEPSSATNASSRSLVDTDDIDENDESGQSILLGIISQLKPGCDLSRITLPTFILEKKSMLERITNQLQFPDILLEAHQEKDDLMRFVGVVKWYLSGWHIAPKAVKKPLNPVLGEHFTCYWDLPNKQQAFYFSEQTSHHPPKSSYFYMIPESNIRVDGTTVPKSKFLGNSTAAMMEGLTVLQFLDIQDSEGHPEKFTLTQPNMYARGILFGKMRIELGDHMVIKSSQHKVDIEFKTKGFISGTYDAIEGTIKDQAGNEYFEISGKWNDIIYIRDLQKKTPKTVLFDASKAAPLKPLVRPLEEQGNFESRKLWKKVTDALAARDHATATEEKFKIEDYQRTLAKKRAESGSPFLPKLFRPASRQSDDLEFYIYKDIPEGGSHEDQIRSILEIAPVLPGQKFTEKFEVPGQQKHKVQKQNST</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGP8</accession>
    <name>C5DGP8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0D07062p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0D07062g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="journal article" date="2016" name="Cell Rep." volume="14" first="2108" last="2115">
            <title>Crystal Structure of the Cohesin Gatekeeper Pds5 and in Complex with Kleisin Scc1.</title>
            <authorList>
                <person name="Lee B.G."/>
                <person name="Roig M.B."/>
                <person name="Jansma M."/>
                <person name="Petela N."/>
                <person name="Metson J."/>
                <person name="Nasmyth K."/>
                <person name="Lowe J."/>
            </authorList>
            <dbReference type="PubMed" id="26923598"/>
            <dbReference type="DOI" id="10.1016/j.celrep.2016.02.020"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (3.20 ANGSTROMS) OF 76-1109</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553028.1">
        <property type="nucleotide sequence ID" value="XM_002552982.1"/>
    </dbReference>
    <dbReference type="PDB" id="5F0N">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.20 A"/>
        <property type="chains" value="A=76-1109"/>
    </dbReference>
    <dbReference type="PDB" id="5F0O">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.50 A"/>
        <property type="chains" value="A=80-1109"/>
    </dbReference>
    <dbReference type="PDBsum" id="5F0N"/>
    <dbReference type="PDBsum" id="5F0O"/>
    <dbReference type="SMR" id="C5DGP8"/>
    <dbReference type="FunCoup" id="C5DGP8">
        <property type="interactions" value="964"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGP8"/>
    <dbReference type="GeneID" id="8295259"/>
    <dbReference type="KEGG" id="lth:KLTH0D07062g"/>
    <dbReference type="eggNOG" id="KOG1525">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002562_1_0_1"/>
    <dbReference type="InParanoid" id="C5DGP8"/>
    <dbReference type="OMA" id="YPPAYNM"/>
    <dbReference type="OrthoDB" id="200660at2759"/>
    <dbReference type="EvolutionaryTrace" id="C5DGP8"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19953">
        <property type="entry name" value="PDS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039776">
        <property type="entry name" value="Pds5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12663">
        <property type="entry name" value="ANDROGEN INDUCED INHIBITOR OF PROLIFERATION AS3 / PDS5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12663:SF0">
        <property type="entry name" value="PRECOCIOUS DISSOCIATION OF SISTERS 5, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20168">
        <property type="entry name" value="PDS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="10 11" id="KW-0002">3D-structure</keyword>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0498">Mitosis</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1113"/>
            <end position="1136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1217"/>
            <end position="1292"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="53"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="1114"/>
            <end position="1130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1217"/>
            <end position="1227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="1249"/>
            <end position="1258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR22590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="10">
        <source>
            <dbReference type="PDB" id="5F0N"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="11">
        <source>
            <dbReference type="PDB" id="5F0O"/>
        </source>
    </evidence>
    <sequence length="1292" mass="146998" checksum="25FD9987AFD47C16" modified="2009-07-28" version="1">MLTRLVSDALHVIKLNRPEQAGNTADYSLMVGSKSRLESDFSIISTIKNNIPTQQLLIRLERLHEELSNLKQESVDLRSLERYRADLIDRKILRNKDHGVRAFAACCLSDILRLYAPDAPYTDKELTEIFRLFLAQLKLLQEPENGYLTQQTYLINNLLEYRSIVILTDLPSSSQLVEELFNIFYSPTNSTIQGNMFTAIGGILGEVISECDSLPMSALKMVFNKFLSHKRAESLDGINYKKDPGFEISLIICQTYSNRLGRHFIKFYSEIMYEVLGESDIHEKGIASSAYKTLVKIGNLTSELWKYAPELVGSVTGLLYQLLCSDNELFRESATKCVSKMLGTHSLINFAVAHSDTYKIWLSKMADISPHVRQAWVSEIPSILMSRSDLSDDISKGLAKALIDSDHTVRLSAIQTFHEVPVKRLWECLPNAAVFAGLVHLTRETRRDLRDECIDAVARIYTESIESIPKTNENKEIWGVVETIPSACFNLYYINDLEINMKVDLLTFEKFLPLGLSNEEFVQRLLTLLQGFNEKAFSSFYAFNRRQDQMSTVLWKFIEFCEETNSQSPAASLSDTKLIKTVEWISSGFPSHLNVEQILLAFRELNDRRLYRLIKVAVAETSKHLTVRNAVSELFKRLEEPELFRKKNIKIESRFTRDNFSTVFRVLIYRAAPIIFNISNLPSFLNTSDSSNEDEKALKRQLIDNISIIKPGIFKDQVKNLVTIITEGETTGPGNTLSLAEAMRTVYKISKDKREHLDIEENTFFFQKLEDYAKEGNPLEAKYAIKLLGLAPNAAEYLSEVATAILPLDLKSKHFASNVLVLAEITKMQPQLLEKDSTEIVGLLIKDVLLSNDVVGDEDDQQAWFSDEDIYTGKADALSAKVFSLKLFANKIKVMAPDAHADEMTHAFTERTLKLFFYLVASGGELVSESNTDNYPTPANYQNKLRCCAGLHILKITKIASLSRFIKPQDISKLMNLVEDESLEVRSSFIGRLKDFLGDGSISIKFLPLVFFTAYEPDQALRTSTKMWINYTLSKENFRKGTFFERALPRLIHFIAHHPDVAEGLRLRETAFLTGLTTAIDYLVFYADSVLKASNLALLYYLAGRVRQYRDAVTEEDNEGEDEDEDEETDQTSHNSSSNIYIISELAQLVLGEFKEHHEWTLSSYPGKLNLPSDLFEPFESIDEARKNTFETYLRTEDTRSLRKVIAAKVSRLYRRTNVHKPLVQKRKNPESSSRPRKRAQASVHSDAELEGESEDDSYVPSRSRRGAVGETRKSSRPKKAVDYADDESEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>C5DH88</accession>
    <name>C5DH88_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Putative transcription factor kapC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0E02222g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Putative transcription factor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the bZIP family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553586.1">
        <property type="nucleotide sequence ID" value="XM_002553540.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DH88"/>
    <dbReference type="STRING" id="559295.C5DH88"/>
    <dbReference type="GeneID" id="8291725"/>
    <dbReference type="KEGG" id="lth:KLTH0E02222g"/>
    <dbReference type="eggNOG" id="ENOG502SC5V">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1053982_0_0_1"/>
    <dbReference type="InParanoid" id="C5DH88"/>
    <dbReference type="OMA" id="AKEYDRM"/>
    <dbReference type="OrthoDB" id="2593073at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14688">
        <property type="entry name" value="bZIP_YAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050936">
        <property type="entry name" value="AP-1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40621">
        <property type="entry name" value="TRANSCRIPTION FACTOR KAPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40621:SF11">
        <property type="entry name" value="TRANSCRIPTION FACTOR KAPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="10">
        <location>
            <begin position="183"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="128"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="26"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="140"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS00036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR23149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="264" mass="27445" checksum="62C198F47123E05C" modified="2009-07-28" version="1">MSNQSLPQMQGVQVLPSLNMRGGGAVNAAPGTGGATAQQQQQQQQQQHQHQQQLQAAVQPPQPQLQTGLVPHGALSAQGQHPGGLALPHPGQPLSMPYIPQVGPHLGLSLGALGGVSGVSGAVNGVRVTSSSATPQPPGAGAGPAAGAAGGAAADARASEGEHLDNSTGQLIGKSGKPLRNTKRAAQNRNAQKAFRQRRERYIKDLEVKAKEYDRMDAQTAALVQENESLKRYVLELEQRLGVRRAASTVGLPHEAHPAIKPPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHH2</accession>
    <name>C5DHH2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">E3 ubiquitin-protein ligase PEP5</fullName>
            <ecNumber evidence="9">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0E04312g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex. Component of the class C core vacuole/endosome tethering (CORVET) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endomembrane system</location>
            <topology evidence="8">Peripheral membrane protein</topology>
            <orientation evidence="8">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Vacuole membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
            <orientation evidence="9">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the VPS11 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="9"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553670.1">
        <property type="nucleotide sequence ID" value="XM_002553624.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHH2"/>
    <dbReference type="FunCoup" id="C5DHH2">
        <property type="interactions" value="656"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHH2"/>
    <dbReference type="GeneID" id="8291819"/>
    <dbReference type="KEGG" id="lth:KLTH0E04312g"/>
    <dbReference type="eggNOG" id="KOG2114">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001287_0_0_1"/>
    <dbReference type="InParanoid" id="C5DHH2"/>
    <dbReference type="OMA" id="ENENECP"/>
    <dbReference type="OrthoDB" id="26184at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033263">
        <property type="term" value="C:CORVET complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030897">
        <property type="term" value="C:HOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048284">
        <property type="term" value="P:organelle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007033">
        <property type="term" value="P:vacuole organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006904">
        <property type="term" value="P:vesicle docking involved in exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057308">
        <property type="entry name" value="CHCR_PEP5_VPS11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000547">
        <property type="entry name" value="Clathrin_H-chain/VPS_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057307">
        <property type="entry name" value="PEP5_VPS11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016528">
        <property type="entry name" value="VPS11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024763">
        <property type="entry name" value="VPS11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23323">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23323:SF24">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 11 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23341">
        <property type="entry name" value="PEP5_VPS11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23356">
        <property type="entry name" value="TPR_PEP5_VPS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12451">
        <property type="entry name" value="VPS11_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17122">
        <property type="entry name" value="zf-C3H2C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007860">
        <property type="entry name" value="VPS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50236">
        <property type="entry name" value="CHCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0926">Vacuole</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="repeat" description="CHCR" evidence="11">
        <location>
            <begin position="405"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="13">
        <location>
            <begin position="928"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="881"/>
            <end position="915"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR007860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR23233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1031" mass="117185" checksum="982E4DF64A226A17" modified="2009-07-28" version="1">MSFQSWRQFQFFENTPVRDPKIGSDSPLFSDPTLSAACPALEDKLFIVVQSRILKLVNLKSLEVELEFEAYSEGFQVTYLRFVADNYIVSAAEQLGRPCSIKIWKLDKQPKNEFDYHSLIEVKNGKNTFPLSAISIWHDLTCLAIGFVNGRIVLIRGDIIHDRGSRQRVIYDDPNKEPITGLMFDRDCRTCFASTTSGIFLFNTTGRNNGKPDTILNGSAGLDLNCSCLSCDGQEFICCSAKSLDFYKSNGEKRSLVMELPLIKRIYAVDQHHILILLGVQTSNNTALNVANITTTNNRVIVLDIQNKLIAMNTLISGSVLDIFSNVFSGDPSVLLLTSDGTMHKINEKPIKEKLQIVEQKELFPVALDLAQQCNLSAIEIEKIRKKFAEHLYKINLKTEAIEQYLQCLNVTETSEVIAKFGINSTSDNSSVSDLSYYLLSMMKKGMSSSDHVTLYLIALIKLKNEEGIEQFVTHFSRSGQYLEEEEPEEDWKQDDESYYFSDTELFDLDLILRLLQDFGFFLQSFKLARKFSKDPAIVVEILIDGLKDPHSALRYIKSLPVDDTLRVLVAFSKKLLELLPNDTNALLIELFTGKYQPLKYESVSDPVVSRREKFDKNVFYSYNSFIEYMKNVSTSKEQDITSSVPTYQPPKPTLIFTSFINQPFEFVVFLEACLGSYNEFHGFDHDKQLILTTLYDIYLTLAKDDEKGRQQEWRDKAHTVYKESTKLATTSTASVDSSSAGRLNKAVDNSLMMLISQVNNFEAALEGDDLQDELEFAKFGKLDLTYKFRSISLTNDAKTSLKFIEKFGEQEPALYSMGLSHFISSKSMMKEIGGEAVFREKILNKVLEKDLMPLLDILQVLGSSNVASFGLVQQILIEHIKSESQEIKNNKKLIQSYESELEEKESKLQSLTDDKSPAQIKLKNQTCNICQTALSAPLVFFKCDHIYHQRCLNEEESSESNNRFFRCPQCVVDFEASENMKRAQSETQIDTELLNLAMNDKDNKDRFKIVTDFIGRGGVESSFIRVQELD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHJ6</accession>
    <name>C5DHJ6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0E04840p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0E04840g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553694.1">
        <property type="nucleotide sequence ID" value="XM_002553648.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHJ6"/>
    <dbReference type="FunCoup" id="C5DHJ6">
        <property type="interactions" value="228"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHJ6"/>
    <dbReference type="GeneID" id="8291843"/>
    <dbReference type="KEGG" id="lth:KLTH0E04840g"/>
    <dbReference type="eggNOG" id="ENOG502S72Q">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112518_1_0_1"/>
    <dbReference type="InParanoid" id="C5DHJ6"/>
    <dbReference type="OMA" id="VYERNYC"/>
    <dbReference type="OrthoDB" id="417450at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.120">
        <property type="entry name" value="BAG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036533">
        <property type="entry name" value="BAG_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003103">
        <property type="entry name" value="BAG_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02179">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00264">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63491">
        <property type="entry name" value="BAG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51035">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="37"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="BAG" evidence="3">
        <location>
            <begin position="114"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR23257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="178" mass="20710" checksum="A6F59FE71F86B19B" modified="2009-07-28" version="1">MDMVRSWLQSPTVKPTKDYVTSSLEPWVRKLRNDNMAMIYTATAVGALLLVLVFKPSKSSKPKKSHKSKGKKAKNPERSVQRKPLTLEEQIEGVHQKFVNEYKEGLDRLAKNYDAKSEKDQYQLNYYNEMLLKLVIELDGVDLVEVEPELKAKLKSRRKQVIHEIQSNLKKLDQLKRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHL4</accession>
    <name>C5DHL4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Mediator of RNA polymerase II transcription subunit 18</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7 8">Mediator complex subunit 18</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">MED18</name>
        <name evidence="9" type="ordered locus">KLTH0E05324g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Mediator complex subunit 18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHL4"/>
    <dbReference type="FunCoup" id="C5DHL4">
        <property type="interactions" value="151"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHL4"/>
    <dbReference type="KEGG" id="lth:KLTH0E05324g"/>
    <dbReference type="eggNOG" id="ENOG502RXWG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058255_1_0_1"/>
    <dbReference type="InParanoid" id="C5DHL4"/>
    <dbReference type="OMA" id="PDRKCMD"/>
    <dbReference type="OrthoDB" id="5348092at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.320.10">
        <property type="entry name" value="Hypothetical Protein Pfu-838710-001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019095">
        <property type="entry name" value="Mediator_Med18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13321:SF2">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13321">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION, SUBUNIT 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09637">
        <property type="entry name" value="Med18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR23275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="275" mass="30101" checksum="9E18083AD9CE43BA" modified="2009-07-28" version="1">MMLQLSLVSSIDDASYDLFVSTLSNLCGNPPVVFGNFTAMLKPNPAFDIEQVNSKNQLVEQSRIKLCRGLPLQEVEADNAKRDFRLLKKYQNDEVQGLDLQIIQDMLHGSRDLDEMEVDGGSNKGGSGAQDVWSLSISDIPTAGSGRKVSTQAITETTVLASGGSSPSIVSFLSELGYVLDFQLLVLGIKFNMNNGVFLELSKIWRLNDGRTEQITKGGFLVKAFVNVAKSTDIKSINSGTTSLLALQKELSGYVELEIPDRKSMDSRLENKNDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHT7</accession>
    <name>C5DHT7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0E07018p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0E07018g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553785.1">
        <property type="nucleotide sequence ID" value="XM_002553739.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHT7"/>
    <dbReference type="FunCoup" id="C5DHT7">
        <property type="interactions" value="57"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHT7"/>
    <dbReference type="GeneID" id="8291940"/>
    <dbReference type="KEGG" id="lth:KLTH0E07018g"/>
    <dbReference type="eggNOG" id="ENOG502S084">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064983_0_0_1"/>
    <dbReference type="InParanoid" id="C5DHT7"/>
    <dbReference type="OMA" id="PPYEREY"/>
    <dbReference type="OrthoDB" id="5801062at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="166"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="362" mass="41126" checksum="A05DC1594CD57D14" modified="2009-07-28" version="1">MEATTRDSPNETELFKTIIEGWDLSQLLKLQVDLAKEIQRRVSGQQNQAADTATKTETQRAKIGGHQQLGQNQPELKYENERSDEFSLKNFTRKSLDKGDDEKATSEAKSSQIGPLDEVSIPGTLDMQVKEIDFSSPLKATDQPNATLQKFSPIKRPLAVISDSEGEIEWSETENSDANFSGSKKQRGEASKLDFNTNPATKKPWIFEDFMANETSASSRKIHSNYESAKVSKFRSAVGQPISNQKLVLHPDRGFEMVEESLREADKAFEGEVQFDNLRQRSDSPPGFGRLDFPNTQESLIDRMKSREILYNKTKARFLEAVRSDLRPDERLFLFRNPSLNEAVNSEDFQWNQSTLDIFSRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHZ6</accession>
    <name>C5DHZ6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 13">Telomerase reverse transcriptase</fullName>
            <ecNumber evidence="2 13">2.7.7.49</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Telomerase catalytic subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0E08470g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. It elongates telomeres. It is a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.49"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Chromosome</location>
            <location evidence="13">Telomere</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 13">Belongs to the reverse transcriptase family. Telomerase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.49" evidence="2 13"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553844.1">
        <property type="nucleotide sequence ID" value="XM_002553798.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHZ6"/>
    <dbReference type="FunCoup" id="C5DHZ6">
        <property type="interactions" value="338"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHZ6"/>
    <dbReference type="GeneID" id="8292005"/>
    <dbReference type="KEGG" id="lth:KLTH0E08470g"/>
    <dbReference type="eggNOG" id="KOG1005">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012565_0_0_1"/>
    <dbReference type="InParanoid" id="C5DHZ6"/>
    <dbReference type="OMA" id="CYDSIPR"/>
    <dbReference type="OrthoDB" id="289721at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000333">
        <property type="term" value="C:telomerase catalytic core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003720">
        <property type="term" value="F:telomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070034">
        <property type="term" value="F:telomerase RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042162">
        <property type="term" value="F:telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007004">
        <property type="term" value="P:telomere maintenance via telomerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01648">
        <property type="entry name" value="TERT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021891">
        <property type="entry name" value="Telomerase_RBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003545">
        <property type="entry name" value="Telomerase_RT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12066">
        <property type="entry name" value="TELOMERASE REVERSE TRANSCRIPTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12066:SF0">
        <property type="entry name" value="TELOMERASE REVERSE TRANSCRIPTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12009">
        <property type="entry name" value="Telomerase_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01365">
        <property type="entry name" value="TELOMERASERT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00975">
        <property type="entry name" value="Telomerase_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 13" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 13" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 13" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="9 13" id="KW-0779">Telomere</keyword>
    <keyword evidence="5 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="14">
        <location>
            <begin position="403"/>
            <end position="704"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU365061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR23407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="854" mass="97569" checksum="E637482FACF21C8C" modified="2009-07-28" version="1">MKTLCEFIWRSAESSNDPQLSKHISEIWEKTDESFREATLHYFVTANCKCWDVPSIPTNDQSSVVDQCVAFLVRKKVFNNVITFGYRAADHSQVASLLHCQSTNLNVTRLKRGIWKTVYGVLGNKRFVELMINHSIFEFGGTAYTQILGIVPREQEKLGSAQCRSSNKAPEYLDSTLYLYKNKGNYSPSKAIPCSPEALWDQMFQIDWKDFELSHHSQLAGVRQLLGQIIKNHQKIKYHHILNNICPRTDMQDDPNNNLCQTDIKLVSRFLVVIFEKTFPLMFFGSRYNRSKILSKIRPLLKLKLHGRMPFSIITEGFRTSDVVWLGKCSKTDPRAALLLQDLITQLVEWVFRRFIPSVISSVFHCCEVSATREIVFFRFDVWKAMADPFLCAYVRDNLKKKSECCCHEAAWKMNHGCLRLVPKKKKKQFRVIFVPLRGCKTEKIAQHEEFIRKTIKPAICVLQFLASETSPLAKKLSSPSDIPSSICLFKETLKKTYGCVPKLYFIKFDIESCYDSIPACKAVKVLEKRLANHSEFFIRSQSYFDPVKRTLKRFSTINAQSTPCEGGVAIDKVQTVHFTKHEILTIIKSEIEKSAIIHEGECYTRKKGIFQGTSLSAAIVDLFYSDLMENETVFSKRNRSESLVLRLADDFLVISISKSYIESVERTVCAGFQEYNAYSNQDKILTHASPRRNEYVRFCAMDLNLQSLEVLKHTETLSTTCAFSSSTRTLYHNLSSNFKKVLSNDLIDPNLNSSDTILKQVFFACINVAESFCLSYVGNPAPLHGFIGFIRKLAEIAEVSCERAFPNQGISSDARKAVFSVFVNFLKDKNMNVQNTSRAIQALLQLTKTINSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHZ9</accession>
    <name>C5DHZ9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SWR1-complex protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0E08536g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the SWR1 complex which mediates the ATP-dependent exchange of histone H2A for the H2A variant HZT1 leading to transcriptional regulation of selected genes by chromatin remodeling. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of selected genes principally by acetylation of nucleosomal histone H4 and H2A. The NuA4 complex is also involved in DNA repair.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SWC4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553847.1">
        <property type="nucleotide sequence ID" value="XM_002553801.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHZ9"/>
    <dbReference type="FunCoup" id="C5DHZ9">
        <property type="interactions" value="1053"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHZ9"/>
    <dbReference type="GeneID" id="8292008"/>
    <dbReference type="KEGG" id="lth:KLTH0E08536g"/>
    <dbReference type="eggNOG" id="KOG2656">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018539_4_0_1"/>
    <dbReference type="InParanoid" id="C5DHZ9"/>
    <dbReference type="OMA" id="GNTTMYQ"/>
    <dbReference type="OrthoDB" id="19740at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000812">
        <property type="term" value="C:Swr1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032563">
        <property type="entry name" value="DAMP1_SANT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027109">
        <property type="entry name" value="Swc4/Dmap1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12855:SF10">
        <property type="entry name" value="DNA METHYLTRANSFERASE 1-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12855">
        <property type="entry name" value="DNA METHYLTRANSFERASE 1-ASSOCIATED PROTEIN 1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16282">
        <property type="entry name" value="SANT_DAMP1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="DAMP1 SANT/Myb-like" evidence="11">
        <location>
            <begin position="108"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="322"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="11"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR23410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="458" mass="52600" checksum="340DFCF82C4A8AAB" modified="2009-07-28" version="1">MASSDIFDVLNIQNPRTSNPGSSAGTPVPSRTPKPQVTGIQRELYNLLGDNTPPVAVQKGSKFKDKLRAIAKPSPWTLAEYEVKPSVKLLHWVKGSKELVEQQPQKSSFSKYNTKLTIPAFSEEEYLSFMGYTTPGSNPLKEWDYEEVQHLFDLCRKYDLRWFIIQDRYEGKLFGTLEDLKDAFYTVCHRYFLAKEPANPLVASLNFSKEKELERKRYLQRLLSRSAAEIAEEEALIVESKKFEMAAKKTLSERETLLRLLDSPNSDKPVSQFLTSQGMTQLYNSLLSDKSRKRKHDQVVPENPWMKQQQQFAQQKQHMQHLQELKSSNSHIQNQVKKTKKQKQEMQIAVKRKADSEYAEQLLQNFTAEERKSLGVQCHGEKLPPGAFLRSSKISTFKAATQSKVAATLQELGLPTRPAMPTMEVINRHEMLLSRITNLLDLKKQLDKLEAEKDIRKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DI48</accession>
    <name>C5DI48_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0E09680p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0E09680g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553896.1">
        <property type="nucleotide sequence ID" value="XM_002553850.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DI48"/>
    <dbReference type="STRING" id="559295.C5DI48"/>
    <dbReference type="GeneID" id="8292060"/>
    <dbReference type="KEGG" id="lth:KLTH0E09680g"/>
    <dbReference type="eggNOG" id="KOG1502">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_9_2_1"/>
    <dbReference type="InParanoid" id="C5DI48"/>
    <dbReference type="OMA" id="CMERKLW"/>
    <dbReference type="OrthoDB" id="2735536at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05227">
        <property type="entry name" value="AR_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF564">
        <property type="entry name" value="STEROL-4-ALPHA-CARBOXYLATE 3-DEHYDROGENASE, DECARBOXYLATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="3">
        <location>
            <begin position="5"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR23459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="360" mass="40243" checksum="F6AC9CF85D6F1646" modified="2009-07-28" version="1">MDEKVLVTGCNGFIALHVLDVLLSEKYHVIGTARSQDKADQVKESFKKLYPYAQLEVEIVPDITKSGAFNDVFKKYPDIQHVLHTASNFSFGHDQSTEEAYLIPATQGTKNILETSLTLGKKVKRFVITSSFASIMDFDHEGDANFIHTEATWNPTTWEKAKGNELSAYIASKKLAEESAWTFMKKHESEVPFTLTTVCPPYVWGPQMFDSSVKRGVLNTSAELINQALKTTPDFKGPFDTPNGIGCDVRDVALLHVLPLRNEKLAGKRLFPVNGTGVKQHNYKNARFNMQRVLDVLNKKFPELRGKISKGGIEDNEKALAAGFYYNNDETCKLTGIEFKPLEVTIHDAAKQILEFGSSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5DI52</accession>
    <name>C5DI52_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0E09768p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0E09768g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAPP small subunits family. BET3 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553900.1">
        <property type="nucleotide sequence ID" value="XM_002553854.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DI52"/>
    <dbReference type="FunCoup" id="C5DI52">
        <property type="interactions" value="76"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DI52"/>
    <dbReference type="GeneID" id="8292064"/>
    <dbReference type="KEGG" id="lth:KLTH0E09768g"/>
    <dbReference type="eggNOG" id="KOG3316">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076409_2_0_1"/>
    <dbReference type="InParanoid" id="C5DI52"/>
    <dbReference type="OMA" id="PYLEIPC"/>
    <dbReference type="OrthoDB" id="941624at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030008">
        <property type="term" value="C:TRAPP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14944">
        <property type="entry name" value="TRAPPC6A_Trs33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1380.20">
        <property type="entry name" value="Trafficking protein particle complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007194">
        <property type="entry name" value="TRAPP_component"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037992">
        <property type="entry name" value="TRAPPC6/Trs33"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12817:SF0">
        <property type="entry name" value="GEO08327P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12817">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04051">
        <property type="entry name" value="TRAPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="Ligand-binding domain in the NO signalling and Golgi transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="252" mass="28431" checksum="B0FFDBB07A123C39" modified="2009-07-28" version="1">MGDSGLASEVNEHQRLQLQFQLFQESLPKVDETIYRMLLNEAVPLAISVEKSLTHHKEDATGQLEAELDSKLALESQQGDASHKLLKDYLAADQDQQTRIKTRLENIGFQLGQKLSQLLIFSNNPNLSFKDMDLLSVMKFVCRDVWKQVFGKQIDNLKTNHRGTFYLLDYNYKPIEPFTLEEDLSDQEQKLIEPFLHVPCGMIRGVLASLGFEKEGQVTCQATLVDPPDSKQAGAGTFTKSVSFNVQVLVGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DI54</accession>
    <name>C5DI54_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0E09812p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0E09812g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553902.1">
        <property type="nucleotide sequence ID" value="XM_002553856.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DI54"/>
    <dbReference type="FunCoup" id="C5DI54">
        <property type="interactions" value="66"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DI54"/>
    <dbReference type="GeneID" id="8292066"/>
    <dbReference type="KEGG" id="lth:KLTH0E09812g"/>
    <dbReference type="eggNOG" id="ENOG502QQW9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028296_0_0_1"/>
    <dbReference type="InParanoid" id="C5DI54"/>
    <dbReference type="OMA" id="ISRYCYW"/>
    <dbReference type="OrthoDB" id="164921at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000322">
        <property type="entry name" value="Integral membrane protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019431">
        <property type="entry name" value="DUF2417"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052370">
        <property type="entry name" value="Meta-cleavage_hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43139">
        <property type="entry name" value="SI:DKEY-122A22.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43139:SF52">
        <property type="entry name" value="SI:DKEY-122A22.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10329">
        <property type="entry name" value="DUF2417"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="95"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="130"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="163"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="191"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="251"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="324"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR23465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="604" mass="69071" checksum="6CE698E6257A55FA" modified="2009-07-28" version="1">MSAAEPNHGVSSGERNNLTGQGAEDRQAQQGLPGTYGHRANENEPLLRETQAKPSHRSIGQVPTDGHLPNEIEGSQGRQFKTRQKYRVFVQDSKIILNVLIFINLVWLITTFIMDYFFNIGFLHFSKRLTSFNDLCLIFVSIVANSVNLWSNRVGLYSTLDQGLNVVLFFMTLFNLFLTLIIGYTRRRLGFVGAFTYIWAALTFFAGAVLDWYLYYYFKEFHAAEEEIEESPSPSGSRHTMKEWVSIGARNFVKCAIFVFYVFFTLNTLLYTIDVGRVTRGVKDVSAKASASYDAFHWVDKEHTYQLHITCYGDFSATDEDRQPVILFDHGSLDTAFLSATWIQELYHLNRVERYCTYERPGYGLSDSAPAPISIAMVADALSYALIKDAKIKGPFTTVGYDIGGLFTQVFTAKNLDKVESMLLVESWHEDILLKNYLQRLLPPDNDSKDPDDISKLPIEIKRHNGFKLWWQGIWSTLGIKLQTSWLLAHHGSKERILGRDMQYQGKFIRAKFLESVTSSLLSYKDVLNSKEKLKDVKTSVASSNQLIKKSPQWGNWQRDITRISSKTQEWKILNGGHEVYKFGSAKQELQDVLLRLIGDKDRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DI89</accession>
    <name>C5DI89_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Porphobilinogen deaminase</fullName>
            <ecNumber evidence="4">2.5.1.61</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Hydroxymethylbilane synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Pre-uroporphyrinogen synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0E10604g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>dipyrromethane</name>
            <dbReference type="ChEBI" id="CHEBI:60342"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 2/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HMBS family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.61" evidence="4"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553937.1">
        <property type="nucleotide sequence ID" value="XM_002553891.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DI89"/>
    <dbReference type="FunCoup" id="C5DI89">
        <property type="interactions" value="690"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DI89"/>
    <dbReference type="GeneID" id="8292102"/>
    <dbReference type="KEGG" id="lth:KLTH0E10604g"/>
    <dbReference type="eggNOG" id="KOG2892">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019704_0_2_1"/>
    <dbReference type="InParanoid" id="C5DI89"/>
    <dbReference type="OMA" id="LWQANHI"/>
    <dbReference type="OrthoDB" id="564646at2759"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00319"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004418">
        <property type="term" value="F:hydroxymethylbilane synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd13645">
        <property type="entry name" value="PBP2_HuPBGD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.40:FF:000002">
        <property type="entry name" value="Porphobilinogen deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000005">
        <property type="entry name" value="Porphobilinogen deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.40">
        <property type="entry name" value="Porphobilinogen deaminase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000860">
        <property type="entry name" value="HemC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022419">
        <property type="entry name" value="Porphobilin_deaminase_cofac_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022417">
        <property type="entry name" value="Porphobilin_deaminase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022418">
        <property type="entry name" value="Porphobilinogen_deaminase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036803">
        <property type="entry name" value="Porphobilinogen_deaminase_C_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00212">
        <property type="entry name" value="hemC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11557">
        <property type="entry name" value="PORPHOBILINOGEN DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11557:SF0">
        <property type="entry name" value="PORPHOBILINOGEN DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01379">
        <property type="entry name" value="Porphobil_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03900">
        <property type="entry name" value="Porphobil_deamC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001438">
        <property type="entry name" value="4pyrrol_synth_OHMeBilane_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00151">
        <property type="entry name" value="PORPHBDMNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54782">
        <property type="entry name" value="Porphobilinogen deaminase (hydroxymethylbilane synthase), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00533">
        <property type="entry name" value="PORPHOBILINOGEN_DEAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="8" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Porphobilinogen deaminase N-terminal" evidence="11">
        <location>
            <begin position="14"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Porphobilinogen deaminase C-terminal" evidence="12">
        <location>
            <begin position="243"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF03900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR23500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="336" mass="37491" checksum="DEF3982FACB2A880" modified="2009-07-28" version="1">MTEEHSAKSEHQIVRIGGRKSKLAVIQSHEVKDLIEREFSQYSCTVRALKTLGDQVQSKPLYSFGGKAVWTKELEDLLYEKDKEKRIDLIVHSLKDMPTLLPDGFLVGAIIKRVDPTDCIIMAKESPYKTLDDLPDGSVVGTSSIRRSAQLKRKYPKLVFESVRGNIETRLSKLDDPITPFSCIVLATAGLLRVKLAHRITQRLDSSVMYHAVGQGALGIEIRSDDPWIAGILQKINDKETAICCFAERSLMRTLEGGCSVPIGVESNYDDTTKQLCLKAVVVSVDGTEAVEDEYTMTIEDMRQDAIICGQQLAHKMIKNGAKDILDRINLDKIDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIH5</accession>
    <name>C5DIH5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0E12562p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0E12562g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554023.1">
        <property type="nucleotide sequence ID" value="XM_002553977.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIH5"/>
    <dbReference type="FunCoup" id="C5DIH5">
        <property type="interactions" value="54"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIH5"/>
    <dbReference type="GeneID" id="8292191"/>
    <dbReference type="KEGG" id="lth:KLTH0E12562g"/>
    <dbReference type="eggNOG" id="KOG0740">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_15_3_1"/>
    <dbReference type="InParanoid" id="C5DIH5"/>
    <dbReference type="OMA" id="YETRLYH"/>
    <dbReference type="OrthoDB" id="10251136at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19509">
        <property type="entry name" value="RecA-like_VPS4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000022">
        <property type="entry name" value="Fidgetin like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000093">
        <property type="entry name" value="Fidgetin-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050304">
        <property type="entry name" value="MT-severing_AAA_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015415">
        <property type="entry name" value="Spast_Vps4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074:SF81">
        <property type="entry name" value="26S PROTEASOME SUBUNIT YTA6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074">
        <property type="entry name" value="AAA DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09336">
        <property type="entry name" value="Vps4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="464"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="170"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="197"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="294"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="216"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="346"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="366"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR23586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="715" mass="80271" checksum="18A8FC311857DC35" modified="2009-07-28" version="1">MPHAKFVVPANLTLRQTLNLLLSIVDNQYRNCQELREKATGDPAILKRLHKALSEALAYLNEGFTQIENFYKVKSVLSLKSPDVLNIVNDLRILDHDLRENQKATEDQLVSGLDEKNQPLSVSNSHFSLSKLLKKTKISSGRKEELRRSEEERKAKEVEELKKQRKLEEARALVEKESQDRKAEEEQARALEMAVKRQVEKEMATRASLERTSTAQRDKSQKTEFEKPQRAFERTSLDVKRSPRRSADQMRRRSLDGGARSKNGSAGLHVRQGSDTGLSAMGNVSKAANLAWSQMKPPLGKPNARVKKPVVLTDGGQSHTTKTRYEYIKPTIKRPSINVPRKASAPRRENSEGAKSREALARSKSPRSPESPAQRSFSPSARSEKSMTPDLDLSPQEKRLKHVMETLEGVDPEACQHIINDILVMGEKVYWDDIAGLNSAKNSLKETVVYPFLRPDLFKGLREPISGMLLFGPPGTGKSMIGKAVATESRSTFFSISASSLLSKYLGESEKLVRALFYLARRLSPSIIFIDEIDSLLTSRSDNENESSRRIKTEVLIQWSSLSSATAREREEGDIESGRVLVLAATNLPWAIDEAARRRFTRRLYIPLPEHETRVAHLKKLLLHQKNNLSDSDFDVIGTLTEGYSGSDITALAKDAAMEPIRELGDRLIDVDFSKIRGINLQDFERAMLTVKKSVSPDSLQKFETWASNFGSQGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIQ7</accession>
    <name>C5DIQ7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0E14410p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0E14410g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554105.1">
        <property type="nucleotide sequence ID" value="XM_002554059.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIQ7"/>
    <dbReference type="FunCoup" id="C5DIQ7">
        <property type="interactions" value="111"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIQ7"/>
    <dbReference type="GeneID" id="8292275"/>
    <dbReference type="KEGG" id="lth:KLTH0E14410g"/>
    <dbReference type="eggNOG" id="ENOG502S5RC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125052_1_0_1"/>
    <dbReference type="InParanoid" id="C5DIQ7"/>
    <dbReference type="OMA" id="GWRDARH"/>
    <dbReference type="OrthoDB" id="4087970at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019434">
        <property type="entry name" value="DUF2423"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28219">
        <property type="entry name" value="UPF0642 PROTEIN YBL028C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28219:SF1">
        <property type="entry name" value="UPF0642 PROTEIN YBL028C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10338">
        <property type="entry name" value="YBL028C_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2423" evidence="3">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="66"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="39"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="18"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="85"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR23668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="108" mass="12592" checksum="868E251F11711E0A" modified="2009-07-28" version="1">MAKSLRAKSQLKAKSVKRKNEFQKAADARSQRLADKMKEDLIRQQIEELKSKNSELKDEDAKLALQDEAMDQDAKKVSTSGWRDARHHNYKRNKKLNKSRKKGSFTKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIT9</accession>
    <name>C5DIT9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Exocyst complex component EXO84</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0E15136g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Secretory vesicle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EXO84 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554137.1">
        <property type="nucleotide sequence ID" value="XM_002554091.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIT9"/>
    <dbReference type="FunCoup" id="C5DIT9">
        <property type="interactions" value="202"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIT9"/>
    <dbReference type="GeneID" id="8292308"/>
    <dbReference type="KEGG" id="lth:KLTH0E15136g"/>
    <dbReference type="eggNOG" id="KOG2215">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014732_0_0_1"/>
    <dbReference type="InParanoid" id="C5DIT9"/>
    <dbReference type="OMA" id="AAWLPNR"/>
    <dbReference type="OrthoDB" id="642193at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030133">
        <property type="term" value="C:transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1220">
        <property type="entry name" value="Exo84p, C-terminal helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1210">
        <property type="entry name" value="Exo84p, N-terminal helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033961">
        <property type="entry name" value="Exo84"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032403">
        <property type="entry name" value="Exo84_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042561">
        <property type="entry name" value="Exo84_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042560">
        <property type="entry name" value="Exo84_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21426">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21426:SF12">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16528">
        <property type="entry name" value="Exo84_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25345">
        <property type="entry name" value="PH_EXO84"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08700">
        <property type="entry name" value="VPS51_Exo84_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst component Exo84 C-terminal" evidence="9">
        <location>
            <begin position="477"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="228"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="415"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="155"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="37"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR23700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="694" mass="78913" checksum="D8D5BB02E3F65488" modified="2009-07-28" version="1">MVELSLRKARNNWKQISSPTKTKTRSRSPANGKFKSNPYQNLTVPSNYSELPQIGTKERNRVGTSMQRRLSFHNPKYVPPSDYSSVPLPQLGSSSLLNVESSNMQARSRSNSTLLRPQQAEVRGDQSLRQILSDSSFKAKEYVAHTLGNASALEIDRFTSELNDLSLEVSDEIKENINRSYAQVLQVNKDLDVASIELKQLRSSISDLEGVMGELESMAEKKLQLERGQANKARPEQASLMPPTKTPKARDRTSVLMLEKMWNNELAALFKSVEGAQKFISPLSGRHILMEAADWYEINAATLKPLRAVHIFVLNDMIMVAARNQDKKQHDLIACHCHALRDTNIDPQKDNRIGLYFSNRNQCLIQTQKTREYDRFVSIVRGAKDDLNVISQAEKENARRLRDSFSYLQATQQTPGRGLTLSPTKGHTRNSSLGNNTARSLNDSSDNYLLQTIAATMQPQTRFSGVELSRGAFGRFDNDVEDFDIVFARHDYREAIHKIESLNKFIQALAKESNQDNYMVLNLLQLKVTYRNQLLTNKLTQLASTETFDFSKLVQYVQHLLLLGYSHDALELFLQNRSKYIHELILKVGSTHNPVIYLTQISVIRFQTLKKVVSSVQELFKNTDTNFSSALVSWCDEEVDKHFALINKQFLNLEAIPPSSIKSSRRQIDDLKSVGLDFVYKLDDFIRRNSQKIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIW0</accession>
    <name>C5DIW0_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">DASH complex subunit SPC34</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Outer kinetochore protein SPC34</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ordered locus">KLTH0E15598g</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
            <location evidence="3">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DASH complex SPC34 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554158.1">
        <property type="nucleotide sequence ID" value="XM_002554112.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIW0"/>
    <dbReference type="FunCoup" id="C5DIW0">
        <property type="interactions" value="47"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIW0"/>
    <dbReference type="GeneID" id="8292329"/>
    <dbReference type="KEGG" id="lth:KLTH0E15598g"/>
    <dbReference type="eggNOG" id="ENOG502QSS0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_970457_0_0_1"/>
    <dbReference type="InParanoid" id="C5DIW0"/>
    <dbReference type="OMA" id="LIRDCNP"/>
    <dbReference type="OrthoDB" id="10016597at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042729">
        <property type="term" value="C:DASH complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005876">
        <property type="term" value="C:spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008608">
        <property type="term" value="P:attachment of spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013966">
        <property type="entry name" value="Spc34"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08657">
        <property type="entry name" value="DASH_Spc34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="16" id="KW-0137">Centromere</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="10" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="12 19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="8" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9" id="KW-0498">Mitosis</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="211"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00044112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00044346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CAR23721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="274" mass="31616" checksum="9F739481757D4D1D" modified="2009-07-28" version="1">MSGDLDDCLDLIAQSSESISTLYFKPPGIFHNAIVPDPNASYADIITKIIRDGDPREEISLYKLDKEGLPKRKDDKMGVFDFLTEYEANLKRNRRIGSVEETPIIHVPKEFYLKQHEQEAKRIRKQEAFILDSVKTDGVLEVLLKRFESDQQVRDLIYALQNGTVITGEDEGGASNRRQTLFVEDFPVELIFKVLNEIVTNWPVSEYQKNYSELLQRFNGIDNEIRQLRSQIKDQEARLSGRQKNSGVARLIEKETQEVQELQRKLGALREQQS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2010-10-05" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJ31</accession>
    <name>IRC22_LACTC</name>
    <protein>
        <recommendedName>
            <fullName>Increased recombination centers protein 22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">IRC22</name>
        <name type="ordered locus">KLTH0F13046g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text>Is probably involved in a pathway contributing to genomic integrity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the IRC22 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554757.1">
        <property type="nucleotide sequence ID" value="XM_002554711.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJ31"/>
    <dbReference type="FunCoup" id="C5DJ31">
        <property type="interactions" value="39"/>
    </dbReference>
    <dbReference type="GeneID" id="8292980"/>
    <dbReference type="KEGG" id="lth:KLTH0F13046g"/>
    <dbReference type="eggNOG" id="ENOG502S3VP">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078554_1_0_1"/>
    <dbReference type="InParanoid" id="C5DJ31"/>
    <dbReference type="OMA" id="WLPETYK"/>
    <dbReference type="OrthoDB" id="1926781at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005595">
        <property type="entry name" value="TRAP_alpha"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03896">
        <property type="entry name" value="TRAP_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000399080" description="Increased recombination centers protein 22">
        <location>
            <begin position="20"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="topological domain" description="Lumenal" evidence="2">
        <location>
            <begin position="20"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="166"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="2">
        <location>
            <begin position="187"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="193"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="221" mass="24497" checksum="855D8EEBFBE9C8C3" modified="2009-07-28" version="1" precursor="true">MKFSGIQVSILALLRVCLAEADSGEGNQEALPHANFDITYECLERDVGGLSSFMEFENGDNATLQYKFTNKEDLNVSVVGLGGRIYDVQKNEDAANISASAIGPLDVDVNGTTEFQHVIKLVLKEGDYVLSPDVYVQKENETMRVVTNPSLIRILPPPMSFFNPKFMLVQMVLGALIAAFSYFTFFKPSASYGRDAKKLKPDGSVSRMDSSWLPENHQKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJD4</accession>
    <name>C5DJD4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">Phosphoribosylaminoimidazole carboxylase</fullName>
            <ecNumber evidence="4 11">4.1.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0F15532g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + H(+) = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + CO2</text>
            <dbReference type="Rhea" id="RHEA:10792"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:77657"/>
            <dbReference type="ChEBI" id="CHEBI:137981"/>
            <dbReference type="EC" id="4.1.1.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">In the C-terminal section; belongs to the AIR carboxylase family. Class I subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.21" evidence="4 11"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554860.1">
        <property type="nucleotide sequence ID" value="XM_002554814.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJD4"/>
    <dbReference type="FunCoup" id="C5DJD4">
        <property type="interactions" value="202"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJD4"/>
    <dbReference type="GeneID" id="8293091"/>
    <dbReference type="KEGG" id="lth:KLTH0F15532g"/>
    <dbReference type="eggNOG" id="KOG2835">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011534_2_1_1"/>
    <dbReference type="InParanoid" id="C5DJD4"/>
    <dbReference type="OMA" id="ITFDHEH"/>
    <dbReference type="OrthoDB" id="15425at2759"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00130"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004638">
        <property type="term" value="F:phosphoribosylaminoimidazole carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006144">
        <property type="term" value="P:purine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1970:FF:000013">
        <property type="entry name" value="Phosphoribosylaminoimidazole carboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000030">
        <property type="entry name" value="Phosphoribosylaminoimidazole carboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000037">
        <property type="entry name" value="Phosphoribosylaminoimidazole carboxylase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01929">
        <property type="entry name" value="PurE_classI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01928">
        <property type="entry name" value="PurK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016301">
        <property type="entry name" value="Ade2_fungi/plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003135">
        <property type="entry name" value="ATP-grasp_carboxylate-amine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033747">
        <property type="entry name" value="PurE_ClassI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000031">
        <property type="entry name" value="PurE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005875">
        <property type="entry name" value="PurK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040686">
        <property type="entry name" value="PurK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054350">
        <property type="entry name" value="PurT/PurK_preATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004679">
        <property type="entry name" value="PRK06019.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01162">
        <property type="entry name" value="purE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01161">
        <property type="entry name" value="purK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11609:SF5">
        <property type="entry name" value="PHOSPHORIBOSYLAMINOIMIDAZOLE CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11609">
        <property type="entry name" value="PURINE BIOSYNTHESIS PROTEIN 6/7, PUR6/7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00731">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02222">
        <property type="entry name" value="ATP-grasp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17769">
        <property type="entry name" value="PurK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22660">
        <property type="entry name" value="RS_preATP-grasp-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001340">
        <property type="entry name" value="AIR_carboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01001">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52255">
        <property type="entry name" value="N5-CAIR mutase (phosphoribosylaminoimidazole carboxylase, PurE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 11" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="10 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="6 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 11" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="13">
        <location>
            <begin position="110"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR001340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR24423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="573" mass="62590" checksum="E4A11260E9E53AF4" modified="2009-07-28" version="1">MDSRTVGILGGGQLGRMIVEAASRLNVKTIILDAPNSPAKQINALTQHVDGSFSNPSDIEEIASKCDVLTVEIEHVDVPTLKKLQKTHPKLEIYPSPEAIELIQDKFTQKEHLIKNEIDVAESVAIESPSVESLAQVGDKFGYPYMLKSRTLAYDGRGNFVVKSKDSIPQALETLNNRPLYAEKWAPFTKELAVMIVRSIDGKVFSYPVVETIHRENICHLCFAPARVPDSIQLKAKLLAENAIKSFPGCGIFGVEMFYLENGQLLINEIAPRPHNSGHYTIDACVTSQFEAHVRAILGLPLPRNFSSLSTSNTNAIMLNVLGDKEKNKELQTCERALQTPGASVYLYGKESRPQRKMGHINIIGSSMEHCERQLRYISGESDELPSSVADNLAPEIPGTSKKPLVGVIMGSDSDLPVMSAACEILNKFEVPYEVTIVSAHRTPHRMSNYAVEAAKRGLKAIIAGAGGAAHLPGMVAAMTPLPVIGVPVKGSTLDGVDSLHSIVQMPRGIPVATVAINNSTNAGLLAVRLLGAYDFTYQEKMQEFLLKQEEEVLVKAEKLENLGYQNYLDGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJG8</accession>
    <name>C5DJG8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0F16324p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0F16324g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554894.1">
        <property type="nucleotide sequence ID" value="XM_002554848.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJG8"/>
    <dbReference type="FunCoup" id="C5DJG8">
        <property type="interactions" value="249"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJG8"/>
    <dbReference type="GeneID" id="8293127"/>
    <dbReference type="KEGG" id="lth:KLTH0F16324g"/>
    <dbReference type="eggNOG" id="ENOG502S1WJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023122_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJG8"/>
    <dbReference type="OMA" id="HDIREPG"/>
    <dbReference type="OrthoDB" id="4703at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000127">
        <property type="term" value="C:transcription factor TFIIIC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006383">
        <property type="term" value="P:transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052416">
        <property type="entry name" value="GTF3C_component"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15052:SF2">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR 3C POLYPEPTIDE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15052">
        <property type="entry name" value="RNA POLYMERASE III TRANSCRIPTION INITIATION FACTOR COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="43"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="75"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="126"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="140"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR24457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="683" mass="76169" checksum="55C6311CB078DA72" modified="2009-07-28" version="1">MARPKGSSGPNEGGKKVRTLDSFNGVKVSSVNRSAAALAVLNSEIASTRTRPRRSASQKASQSLAEMIDPHVGSLDDDEDFEPPGEQGDVEGAEAEEEYEERDDLEDDELDNPIEKPSTRAKKTKSTSAAAGVPKPRATGAKRGRKPKKAASVNEEIKALTPTSKLDNKHRIIRGLKDLTSARDKIERIYGLNEQKLLGLAKVKEGFEAGPFDFDLQTIQEDSKYFVDSSPPWSKENIAHAIRLKKAKFDLMEESDLNYLFPVRQSELRLIIGDLDTAIATGQKVEFPVLPCGKRKGFIYSTGALVTDIAWLPRDLNGELFLAVSMSKYFDDPADPHLRLFSKEEHISCVTIFRLDPNTLSFEKYQTIVHQFGETWGLKWHEAYQDDQSLGLLAACCQDGTVKFIKVNLVQNYEIIMLQEASLEISIPQGLISCFDFTSSLSIICGFQNGYVAEFELGSNLPSFYYKVHDSYIISIVTAYSQYEDIIINTISVDGFTCAFNPKSIRTTKSIVGRARGGNNTPAVYCPQLYCVAYSDGVNSVKAYPPRAVFATHQICMHDNTVSSLAASKRHPYLLSGSADGALMINNMARRFLTGIKNNTTVYKYLKLWQWDYSLKEDKYRLDPNYEVYNFSVNEVSKAKVDPHGINISSVKWNETTKFGKFFAFVNNAGMMVVEELGSEQQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJK0</accession>
    <name>C5DJK0_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">ATP-dependent RNA helicase DBP5</fullName>
            <ecNumber evidence="4">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">ATP-dependent RNA helicase dbp5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="ordered locus">KLTH0F17050g</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the DEAD box helicase family. DDX19/DBP5 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="4"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554926.1">
        <property type="nucleotide sequence ID" value="XM_002554880.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJK0"/>
    <dbReference type="FunCoup" id="C5DJK0">
        <property type="interactions" value="835"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJK0"/>
    <dbReference type="GeneID" id="8293159"/>
    <dbReference type="KEGG" id="lth:KLTH0F17050g"/>
    <dbReference type="eggNOG" id="KOG0332">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_1_0_1"/>
    <dbReference type="InParanoid" id="C5DJK0"/>
    <dbReference type="OMA" id="IAAETRW"/>
    <dbReference type="OrthoDB" id="10265785at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17963">
        <property type="entry name" value="DEADc_DDX19_DDX25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
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        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
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    <sequence length="478" mass="53196" checksum="E606A5E73FD21C22" modified="2009-07-28" version="1">MTDTSKKADAADLLASLNIESKKDQAPDAKTVEQKEQSAPEEQGVPKDETADQKKKEQDSNLVKSEYEVKVKLADLQGDPNSPLYSIKSFEELGLAPELLKGLYAMKFQKPSKIQERALPLLLHNPPRNMIAQSQSGTGKTAAFSLTMLSRVDPAVPQTQAICLAPARELARQTLEVIQEMGKFTKISSQLIVPDSFEKNKPVNAQIVVGTPGTVLDLMRRKMIQLGQVKVFVLDEADNMLDKQGLGDQCLRVKKFLPKSAQLVLFSATFDDGVRNYARKVVPDANSLELQRNEVNVAAIKQLFMDCTDENNKFEVLTELYGLLTIGSSIIFVQTKQTANVLYAKLKQAGHQVSILHGDLQASERDRLIDDFREGRSKVLITTNVLARGIDIPTVSMVVNYDLPTTFQGKADPSTYIHRIGRTGRFGRTGVAISFVHDKKSFEVLTAIQKYFGDIEMTRVPTDDWDEVEKIVKKVLKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
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    <name>C5DJR6_LACTC</name>
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            <fullName evidence="7">KLTH0F18546p</fullName>
        </submittedName>
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        <name evidence="7" type="ordered locus">KLTH0F18546g</name>
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        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_002554946.1"/>
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    <dbReference type="OMA" id="PNYFAKP"/>
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        <property type="component" value="Chromosome F"/>
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        <property type="entry name" value="FMP27/BLTP2/Hobbit_GFWDK_RBG"/>
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        <property type="entry name" value="FMP27_SW_RBG"/>
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        <property type="entry name" value="FMP27_WPPW_RBG"/>
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        <property type="entry name" value="Hobbit"/>
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        <property type="entry name" value="ANTIGEN MLAA-22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN FMP27, MITOCHONDRIAL"/>
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        <property type="entry name" value="Fmp27_WPPW"/>
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    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
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            <end position="45"/>
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            <begin position="1148"/>
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            <begin position="1842"/>
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            <begin position="1095"/>
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            <begin position="2474"/>
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            <begin position="2491"/>
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            <begin position="2627"/>
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        </source>
    </evidence>
    <sequence length="2777" mass="317179" checksum="E485D92F83F084D1" modified="2009-07-28" version="1">MSWEQTTDFALKHKYIILVFVEAAVGWAVFQWILFKLLGISVGIIDPLRLRVRSLRFREILRIRFVYYSLWNHGIVVHGLQLNLPYKRQVKESAKSNKPSPKNYFSVPRWAQWLIIRFLNLFKEYNFVLEKIEINGVYVELLTAKLVGNREHMRVSIYMKDVTKTDIMKCDGTLLEFSGRINFQHPILPLEDMSIDIKLRDVSISYDQLTKSIESAYQQKAAERSEAPANESHKDHFQTNMQPVTTGESIEYIKSLLLKASDFVTMFDRFYVALDTVCLADIPFTRHPELRKTADHLKFKVDASNVTLSINRLKVGSPGFKLLFSPGDSPLSLKSTISSIAFSSSRVEDDKALSSKPYNFCEIPSVSLYGDTNLLSLRSLEEDGNPLNTVLKLVGHVSSPVVDFEISQLSFMKAFKDNLKVFQSLLSDDITVPVVESRYEQTLKRKQAVASYFQHILPMIESKVTIEDPMILISDDCELLVQKCSILSIQTRSTKYALGGEQKKEEVFYKLRNSVEMTDYNFTYQDKVRKFEHQILAIDSVSMLSFSQLIPIPLVGCTINVDVFNLDLSELSTLMALNKIFRKTNSKMLLVEDEYFDDLYCKFSEMLEAAAQKGSKLDFQCNETDDCPTSLLFKPLPSFFDGIRGCVCGVSITVGARSVFMSKDVLTDLRPQSPEDLVNGELRKMSHSVEKVSFYLSSSNSAAPSMNDVGGSDDSTLAESAKMFSYDELGLEDSNSSFSSSKDCVWLFRCVFQDMVTTLYSEKRTKRQALCAKTICKLPEFEASVYPSGPLDEKGEVGSNKILTRFGFSECEMMFSLMTLFLVFSAIHTFRQTFASDVNKHGRDSKAKKHLALDLRKPSQKSPLSRLIKTDTLDVFQVDFNAKALNMVIILPNGVKTRLEMFQTSLGWANISEISLQGHFLRFCVESPQITNTWVRMATVVNFKIEGDIYHIFEQIQKPPEGTPKPSVVLSNEMWHFNIPHGFAMCRIIDNITTTAKSIKQMVHSLKTSERDSVIHPHVSNPVLFPVITLKSYRWIFSVEDDAFESQLGMIFQVGLEEQRSRIEKYNLFERSIAKELSTRQKGLERRETTLVNLKEKSDQDHAPEDKAPSRNNSESMLPRQGVEWATQESDRYKEYHQFLENAETRAKEFYALQKQISLSWIRRIQSFKMKERIDFSKNFEYLWGKINPNCFPPGANKKIVAFLSSPPLMNMIIERINVTISQPSFGIEKVPDFINDVGKGVPRETKYSIMVPMHLNAEFGEIRSHLKDYPLPFVHIPDFTDSQSDADFVPIHIHGDIIISEDKLWSEKELRTLFVPLVPSATLENDDELYSILVPRTLTAIKTYTSLEFELNSEETTMVTWNGSYSPAIQQVMQCFENFSKPPIDPSPKLGFWDKIRETFHARVAVKWNRGGQLNVALKGGKSPYMMGGESAGFIVGLKGKVAVGCNKSDDSKKFVSLASDEIFFSIPNYFAKPLFSWSSSSKNFFFFPSYDNTNLQQHAFFYHLIDLPDANSLSQDMKVMRDSFFEKTAIRLTGGITLNVGIVFERMKNGRDCRTLESKPHWETRLCNPVFVKELSKHDSFGGFRSDFIHLSFTLLSSNENAYNVLQLTPGGFKTFFGWWKSFSGILPVRRGPLFGMDSMSPKFGLHLYTISYHADVAPLFISHIHNVFDLNSDARKKDGQSTDFVGLKAKTEHFVMDLHQRREVMHEYKPVLETTKKNSKLAFNGGDVSTFEIDVRTVRAQFKESSSVDRESSLFEISDDDMTWFDVADFKEVHCDSLEDRIPSVSIDSLVYSPKFVYRKHASYGDKYQVDFETCEKIEPFQNSNYHDCSLHDNVRSPVYLIDNRLKALEAKRKEVENQINEISTESGKNKRDLSLKLGKIKEAISQVSKLLTDFEEFEDYDVTEQGDVSASNTSNEKDNDYAECEWLTKKSFSKTFEHRFFIFCMFLKWNETVRDTVYSYVHLIDLNREFAYITNHKAVQKLEDFLRDNADENIKKLSTNETSKHEKTPSLMNESVRSLDANEDILSVFEEGLRTLNAGFDYITHDNHVVQFIAPQIQLTTTRKSEVCTMITAPSIKLKTIGFDTNTTENENNSDVFMNRYSVALLKANVFVFHKDNFENYHDVFFSSEGYDQLKGDNWQPWLGVELCFDSKALVNDALIRDLSGIFRFDRVFSFANISSSGDTKLENRMICQLPRTIISSNSRQYLALYDVVTHLLIYVEPKSAHLRKEVEKLMLSYDSTNIAHLKKVAMDLQQKVVALNLIEKELTFRRPILEADGIDDLKTIRKNKNESLIGLYILMKVLNTGSQEQAANDQRMLWDLEAKEIILHMLHEDGGPFLDVALAKSFFQRIQSSYGYNSNRVVIGIAQIFNLDRDVLFHSLMSPSEGRKGELDHGLSKKPMIELEWEIERPVGGMKVIKNAISAFQGLDINVEQETLSKIVKWVFPSEIEQYIDGPQKQASAKESDDDVTSSMSGQQSLTIGEINDDPQSSQPDPQDVNEMFKRSSDYMVIDRMVVNSFVLTISYRGHGAKRLINVTHFGFLFPHLTIINQTMRMVDLVMVLKQVILKSLLKHAGKFLGNKLKKHNVRVDSPGPQLQQLSSYRTFTNVDDLKVKGPQKKTVSEHQSSTQSPSENQSNDQGYAVPEKNAHEGYVHQEKAIEKPPAKGQDASEEPQSFSNIEEEASKHDQKSLKIGQVSGLSVHENKKHHARTQEIEQNQQEQTAIKDRASSGKELDWLQNHNTSQNKKRSSTVQGEKHNREKEQVPIEKSHSLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJR8</accession>
    <name>C5DJR8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">GTP-binding protein RHO1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0F18590g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554994.1">
        <property type="nucleotide sequence ID" value="XM_002554948.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJR8"/>
    <dbReference type="FunCoup" id="C5DJR8">
        <property type="interactions" value="832"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJR8"/>
    <dbReference type="GeneID" id="8293229"/>
    <dbReference type="KEGG" id="lth:KLTH0F18590g"/>
    <dbReference type="eggNOG" id="KOG0393">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041217_21_2_1"/>
    <dbReference type="InParanoid" id="C5DJR8"/>
    <dbReference type="OMA" id="EERPQMA"/>
    <dbReference type="OrthoDB" id="8830751at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006075">
        <property type="term" value="P:(1-&gt;3)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01870">
        <property type="entry name" value="RhoA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000329">
        <property type="entry name" value="GTP-binding protein rhoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0636">Prenylation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00067971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR24557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="208" mass="22666" checksum="EEF38BD70B31F26B" modified="2009-07-28" version="1">MSFQAGSGIRKKLVIVGDGACGKTCLLIVFSKGQFPEVYVPTVFENYVADVEVDGRRVELALWDTAGQEDYDRLRPLSYPDSNVVLICFSIDLPDSLDNVQEKWIAEVLHFCQGVPIILVGCKVDLRDNAQTVETLRAIGQQPVSASEAQEVANKIGAVSYYECSAKTGYGVRDVFEAATRAALSGKATLKQSKAGAKQKKKKKCVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJS2</accession>
    <name>C5DJS2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Mediator of RNA polymerase II transcription subunit 10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">Mediator complex subunit 10</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MED10</name>
        <name evidence="10" type="ordered locus">KLTH0F18678g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the Mediator complex subunit 10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJS2"/>
    <dbReference type="FunCoup" id="C5DJS2">
        <property type="interactions" value="626"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJS2"/>
    <dbReference type="KEGG" id="lth:KLTH0F18678g"/>
    <dbReference type="eggNOG" id="KOG3046">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096169_1_0_1"/>
    <dbReference type="InParanoid" id="C5DJS2"/>
    <dbReference type="OMA" id="QYQRAKM"/>
    <dbReference type="OrthoDB" id="337270at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019145">
        <property type="entry name" value="Mediator_Med10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13345">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13345:SF13">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09748">
        <property type="entry name" value="Med10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0010">Activator</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR24561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="151" mass="17388" checksum="2C46A0537DECE145" modified="2009-07-28" version="1">MPPADVNKMQEDLSLSEQQLRSIIESFVELGVSVYDFPGTLEATQGMMTNLKRNVDRLRQLNKQSNDPESQLNNFNVPIEVAQYIEDGRNPDVYTRDFVEAIRRSNQYQRAKMNALGQLRNKLAEKIIAEFPDMQNNVTDILARTRREPSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJX4</accession>
    <name>C5DJX4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0F19844p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0F19844g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555050.1">
        <property type="nucleotide sequence ID" value="XM_002555004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJX4"/>
    <dbReference type="STRING" id="559295.C5DJX4"/>
    <dbReference type="GeneID" id="8293286"/>
    <dbReference type="KEGG" id="lth:KLTH0F19844g"/>
    <dbReference type="eggNOG" id="KOG2348">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010778_2_1_1"/>
    <dbReference type="InParanoid" id="C5DJX4"/>
    <dbReference type="OMA" id="AWTWPAT"/>
    <dbReference type="OrthoDB" id="6132759at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015489">
        <property type="term" value="F:putrescine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015606">
        <property type="term" value="F:spermidine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015204">
        <property type="term" value="F:urea transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11476">
        <property type="entry name" value="SLC5sbd_DUR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031155">
        <property type="entry name" value="DUR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46154">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46154:SF4">
        <property type="entry name" value="UREA ACTIVE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="54"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="156"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="192"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="249"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="291"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="398"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="423"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="451"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="491"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="559"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="587"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="640"/>
            <end position="665"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR24613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="665" mass="71256" checksum="2E7AFB738969757F" modified="2009-07-28" version="1">MEPVLSQGYGYGFALGIGAGFALLMVIISKLLSKYMGQVQNSERFSTASRNVESGLIASSTVSAWTWPATLLLSGTWAYVHGVSGAWLYAVGGTVQITLFAFLALQIKLHAPNAHTVSETLYVRFGKTGHILYLCYCAATNAMVSSLLLLGGSQAFAAATGMHTVAASFLLPLNVVVYTALGGLKATFISDWVHTVIIYVIILTTSYTVYCNSSLIGSPGKMWDLLKSAQEVFPSASGTSYLSFKDKDMILLTWSVMLGGFSSVFGDPGYSQRAIASSSRSVFSGYMMGSICWMVIPFALGSSAGLACRALLTHPKSVTYPNALSAEEVGAGMPLIYGLASIFGKSGAAAGLLMLFLSVTSATSAELIAFSSITTYDLYRTYLNPRASGQQLVRASHLSVISFGIFMAVLAVIFNYINVTVSWLLSFVGIVLNPEAAAVTLSLFWPRMTKASLLIGMPLGTVTGIVCWIGATYSYADGVINRDTLMTTEATFIGNIVSLFSCAIYIVGISLIWPSGKFDMATWKEQIQTADDMDSEELEALVVTQDDARLLRTQSWLSLLINVFLLIGVYVVLTCALYGWGGDLSKGSFTAFMIVMLVWLMLAALYIILTPLWQGRKAIATISLLVCGKAAPVVASSAASMRGSESLNKSEPKDESFAVQTQEVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJY5</accession>
    <name>C5DJY5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">KLTH0F00330p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0F00330g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554223.1">
        <property type="nucleotide sequence ID" value="XM_002554177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJY5"/>
    <dbReference type="GeneID" id="8292413"/>
    <dbReference type="KEGG" id="lth:KLTH0F00330g"/>
    <dbReference type="eggNOG" id="KOG0758">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015166_16_2_1"/>
    <dbReference type="InParanoid" id="C5DJY5"/>
    <dbReference type="OMA" id="ATFVTFE"/>
    <dbReference type="OrthoDB" id="409586at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000064">
        <property type="term" value="F:L-ornithine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990575">
        <property type="term" value="P:mitochondrial L-ornithine transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000163">
        <property type="entry name" value="Mitochondrial carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624:SF51">
        <property type="entry name" value="CARRIER PROTEIN YMC2, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="24"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="116"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="213"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR23786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="303" mass="33568" checksum="3ACFC6D5BDC8C54A" modified="2009-07-28" version="1">MSDELTMPQVVDDLADKSDFHRATKDVLAGISGGIAQVLVGQPFDITKVRLQTSSTSTTAFKVIKDLIKNEGVSAFYKGTTTPLIGVGLCVSSQFGTNEAMKRYFHRKNNFQSPTLRLSEYYVCGFVSGSANAFLATPIEHVRIRLQVQTKSLAEAEYRGAIDCIKKLLAEGKLMRGFTPTISRTSHGFGVYFLSYETMICGERKRGVARENIPAWKLCLFGALSGSLLWMMVYPFDVIKSVMQTDKLRKPVYGNNVVEVAKNIYRERGAGAFLKGFGPTMLRSLPVNGATFTTFEMAMRLMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKG5</accession>
    <name>C5DKG5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0F04488p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0F04488g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554403.1">
        <property type="nucleotide sequence ID" value="XM_002554357.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKG5"/>
    <dbReference type="FunCoup" id="C5DKG5">
        <property type="interactions" value="39"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKG5"/>
    <dbReference type="GeneID" id="8292598"/>
    <dbReference type="KEGG" id="lth:KLTH0F04488g"/>
    <dbReference type="eggNOG" id="ENOG502S6IX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114202_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKG5"/>
    <dbReference type="OMA" id="YWQPHEL"/>
    <dbReference type="OrthoDB" id="4035013at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="167"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR23966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="195" mass="22294" checksum="BEB7660FC2B504B4" modified="2009-07-28" version="1">MARLASPLTVVEYALPPFERQTKSYVCECDSCRICVEYRFLLPRLFFGGFILPTCWILNIALFVYMQEIVEHAVKFPQLAPEDFPTLFELEMYQKRVPIAPEKIRHQRAAENAGSEETKPGQAPVTNLAATDPLGKLLLDSRLEFLTVVSGDVLASHAENRYYFQSWLWRTAFSLTGYILLLTFVIVALRKSQNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DL08</accession>
    <name>C5DL08_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0F09020p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0F09020g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554596.1">
        <property type="nucleotide sequence ID" value="XM_002554550.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DL08"/>
    <dbReference type="FunCoup" id="C5DL08">
        <property type="interactions" value="329"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DL08"/>
    <dbReference type="GeneID" id="8292802"/>
    <dbReference type="KEGG" id="lth:KLTH0F09020g"/>
    <dbReference type="eggNOG" id="KOG0381">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076155_0_0_1"/>
    <dbReference type="InParanoid" id="C5DL08"/>
    <dbReference type="OMA" id="DKWKQAY"/>
    <dbReference type="OrthoDB" id="5550281at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050342">
        <property type="entry name" value="HMGB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112:SF24">
        <property type="entry name" value="HIGH MOBILITY GROUP PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112">
        <property type="entry name" value="HIGH MOBILITY GROUP PROTEIN DSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="4">
        <location>
            <begin position="108"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="2">
        <location>
            <begin position="108"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="186"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="214"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="233"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR24159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="250" mass="28110" checksum="1C9520DFF68A7A2C" modified="2009-07-28" version="1">MSEPSIKLRTAKDTLVSSLFELSKSANQTASSIIDFYNAVGEDEDEKLEAFTTLTEALQKLTSATNQVQVVSSELTNPLEDDKEVIASIASPVKAPARKRAERDPNAPKKPLTVFFAYSAYVRQALREERQKAGLPPLSSTEITQEISKKWKEMSDVEKDKWKQAYNVELQNYHLEKLKYLEDKKNGVAPSQDAPSSAPVPIPDYLQTELNEFEPPKEKRPHEDEGNGGSSEKKKKKKKKDKKKDKHSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLD4</accession>
    <name>C5DLD4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-directed RNA polymerases I and III subunit RPAC1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">DNA-directed RNA polymerases I and III 40 kDa polypeptide</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0F12056g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the archaeal Rpo3/eukaryotic RPB3 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554722.1">
        <property type="nucleotide sequence ID" value="XM_002554676.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLD4"/>
    <dbReference type="FunCoup" id="C5DLD4">
        <property type="interactions" value="1114"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLD4"/>
    <dbReference type="GeneID" id="8292936"/>
    <dbReference type="KEGG" id="lth:KLTH0F12056g"/>
    <dbReference type="eggNOG" id="KOG1521">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038421_0_1_1"/>
    <dbReference type="InParanoid" id="C5DLD4"/>
    <dbReference type="OMA" id="KKKCRAF"/>
    <dbReference type="OrthoDB" id="270173at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006383">
        <property type="term" value="P:transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07032">
        <property type="entry name" value="RNAP_I_II_AC40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.120.12:FF:000003">
        <property type="entry name" value="Dna-directed rna polymerases i and iii subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.10:FF:000005">
        <property type="entry name" value="Dna-directed rna polymerases i and iii subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.120.12">
        <property type="entry name" value="DNA-directed RNA polymerase, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.10">
        <property type="entry name" value="RNA polymerase, RBP11-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00320">
        <property type="entry name" value="RNApol_arch_Rpo3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011262">
        <property type="entry name" value="DNA-dir_RNA_pol_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011263">
        <property type="entry name" value="DNA-dir_RNA_pol_RpoA/D/Rpb3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022842">
        <property type="entry name" value="RNAP_Rpo3/Rpb3/RPAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033901">
        <property type="entry name" value="RNAPI/III_AC40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036643">
        <property type="entry name" value="RNApol_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050518">
        <property type="entry name" value="Rpo3/RPB3_RNA_Pol_subunit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001988">
        <property type="entry name" value="PRK00783.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11800">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11800:SF13">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01000">
        <property type="entry name" value="RNA_pol_A_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01193">
        <property type="entry name" value="RNA_pol_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00662">
        <property type="entry name" value="RPOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56553">
        <property type="entry name" value="Insert subdomain of RNA polymerase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="RBP11-like subunits of RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase RpoA/D/Rpb3-type" evidence="8">
        <location>
            <begin position="67"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00081520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR24285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="350" mass="39509" checksum="4C0FC21DE203AC62" modified="2009-07-28" version="1">MSTRSIGRQRIHYTNTMSNIVGIEYNRVTNTTSTDFPGFEKDQDAAWNLERFKNTFDVNITSLDDREANFDLVNVDASIANAFRRIMIAEVPSVAAEYVYFFNNTSVIQDEVLAHRIGLTPLKVDPDMLSWVDTSVPEAERFTDENTIVLTLNVRCTRNPDAPKDCTDPKILYRNAHIYARDLKFEPQGKQRETFAKNPVVPADPDILLAKLRPGQEISLRAHCVLGIGSDHAKFSPVSTASYRLMPHINITQPITGESAQRFAKCFSPGVIGINDNGEAFVKDARHDTVSREVLRHEEFEGKVKLGRVKNHFIFNVESAGAMTPEEIFFKSVRILKNKAEYLKNCPIAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLH2</accession>
    <name>C5DLH2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0G00704p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0G00704g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RMD1/sif2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555070.1">
        <property type="nucleotide sequence ID" value="XM_002555024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLH2"/>
    <dbReference type="FunCoup" id="C5DLH2">
        <property type="interactions" value="206"/>
    </dbReference>
    <dbReference type="GeneID" id="8293328"/>
    <dbReference type="KEGG" id="lth:KLTH0G00704g"/>
    <dbReference type="eggNOG" id="KOG2861">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011220_3_0_1"/>
    <dbReference type="InParanoid" id="C5DLH2"/>
    <dbReference type="OMA" id="FHFEYDM"/>
    <dbReference type="OrthoDB" id="18302at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003734">
        <property type="entry name" value="DUF155"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051624">
        <property type="entry name" value="RMD1/Sad1-interacting"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16255">
        <property type="entry name" value="REQUIRED FOR MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16255:SF4">
        <property type="entry name" value="SPORULATION PROTEIN RMD8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02582">
        <property type="entry name" value="DUF155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="582"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="domain" description="DUF155" evidence="4">
        <location>
            <begin position="379"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="295"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="318"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="346"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR24633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="615" mass="70029" checksum="2FEC3F3A08247089" modified="2009-07-28" version="1">MQSSLGFQEGPGRRTSMSRRSPSILVTDARSSKKRIGAPFAGHAAGLRGGRAAREKSDTYQGFSASSRGSMRGNSASGSDLGPMRRSRAKLRQQGKFSDISIDNILSDVSDVPSGRREERLSISSHEMAPFAHHSSPGQYTKLINPLPPRTSKTSKKLVLIPEDSDRKNNALQMERRRPLKYNRHDPDGQENYDEEMSRITAYNVAEGFEMSSTSDFLRYTHDVSPRLYDECLYVPYCLPLLPGKDGFRIKSNVSKKTIGGKTLIDRLIDKSEQRDHHYEYYSGVETVEDMNNNFELSDTAGKDNERNDSLPTPDHLPNNSSNNTASFDPSEPQFFAEEGRDTDQSKNISAVGNRSQNPYMESQSLENYGSSKQQHAEIFIFSYGVVVFWNFTEIQEKNILGDIAFAEHDNMIIRPLDEQDIETEQFHFEYDMNVERPRIFNDIVTLRSGDHIIKLTLSHSIAQSTKLSRFESRISPILGTVSKLPKRLALYGTLGLKREQLLKKSGKLFKLRVDVNLSSSVLDTPEFFWSFEPSLHPLYLAMREYLEIDQRVQVLNDRCKVFLEFFDICVDSVAERNMARITWWFIAVIFVSVLVSICEIIVRSFIIRQLKNSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLI7</accession>
    <name>C5DLI7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0G01034p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0G01034g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555085.1">
        <property type="nucleotide sequence ID" value="XM_002555039.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLI7"/>
    <dbReference type="FunCoup" id="C5DLI7">
        <property type="interactions" value="937"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLI7"/>
    <dbReference type="GeneID" id="8293343"/>
    <dbReference type="KEGG" id="lth:KLTH0G01034g"/>
    <dbReference type="eggNOG" id="KOG3943">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039352_2_2_1"/>
    <dbReference type="InParanoid" id="C5DLI7"/>
    <dbReference type="OMA" id="MNEKACV"/>
    <dbReference type="OrthoDB" id="367221at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11717">
        <property type="entry name" value="THUMP_THUMPD1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2300.10:FF:000001">
        <property type="entry name" value="THUMP domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2300.10">
        <property type="entry name" value="THUMP superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004114">
        <property type="entry name" value="THUMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040183">
        <property type="entry name" value="THUMPD1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13452">
        <property type="entry name" value="THUMP DOMAIN CONTAINING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13452:SF10">
        <property type="entry name" value="THUMP DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02926">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00981">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143437">
        <property type="entry name" value="THUMP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51165">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="THUMP" evidence="2">
        <location>
            <begin position="130"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR24648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="276" mass="31683" checksum="BAE68F90D8727295" modified="2009-07-28" version="1">MLLTSAQKYKFSTGIIEPCVSGIYATCARKHEKQAAQELTQLFQEKLEEWYGDELRELGHGESDDESDNDASVEDKIKKELEALHAKNNGPKSKEILRFVDLNCECVVFCKTRKPIDPEKFVHQLMQEFANPKESQKRTRYVQKLTPVTFSCNASLPELTKLSQRVLKPHFHEEGTKPLKFAIEVTRRNFNTLDKMDIIKEVARVAGNGGQLPHKVDLKNYDKLILVECFKNNIGMSVVNSEYRDDFKKYNIQQIFESKIKAQAANATQDVKLEPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLI8</accession>
    <name>C5DLI8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">ER membrane protein complex subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0G01056g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the EMC4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555086.1">
        <property type="nucleotide sequence ID" value="XM_002555040.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLI8"/>
    <dbReference type="FunCoup" id="C5DLI8">
        <property type="interactions" value="791"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLI8"/>
    <dbReference type="GeneID" id="8293344"/>
    <dbReference type="KEGG" id="lth:KLTH0G01056g"/>
    <dbReference type="eggNOG" id="KOG3318">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098404_1_3_1"/>
    <dbReference type="InParanoid" id="C5DLI8"/>
    <dbReference type="OMA" id="QQTFKVI"/>
    <dbReference type="OrthoDB" id="369569at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009445">
        <property type="entry name" value="TMEM85/Emc4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19315">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06417">
        <property type="entry name" value="EMC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017207">
        <property type="entry name" value="UCP017207_TM-p85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="14"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="38"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR017207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR24649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="181" mass="19758" checksum="8BE42C3E887CAD21" modified="2009-07-28" version="1">MSDIIPEWAHTLCDPNHPKKAPAVNSKSLPSPPGFKSLSGSSSKDTKASLASGQRQDVAALIVQKAWQIAFQPAKSIPMNMIMSYMSGTSLQIISIMTALMFVSNPVKSIANMRQTFKPVMGNAEAQPQVVVAMVMFVFFQIILMGIGIHKLNSMGLIPNTRSDWLLLEQPAVYKGKSYAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLM5</accession>
    <name>C5DLM5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">FACT complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">KLTH0G01870g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the FACT complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the peptidase M24 family. SPT16 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555123.1">
        <property type="nucleotide sequence ID" value="XM_002555077.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLM5"/>
    <dbReference type="FunCoup" id="C5DLM5">
        <property type="interactions" value="1518"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLM5"/>
    <dbReference type="GeneID" id="8293381"/>
    <dbReference type="KEGG" id="lth:KLTH0G01870g"/>
    <dbReference type="eggNOG" id="KOG1189">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004627_1_0_1"/>
    <dbReference type="InParanoid" id="C5DLM5"/>
    <dbReference type="OMA" id="YHINTIP"/>
    <dbReference type="OrthoDB" id="10251642at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035101">
        <property type="term" value="C:FACT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034728">
        <property type="term" value="P:nucleosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01091">
        <property type="entry name" value="CDC68-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.150:FF:000002">
        <property type="entry name" value="FACT complex subunit SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.210:FF:000001">
        <property type="entry name" value="FACT complex subunit spt16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000017">
        <property type="entry name" value="FACT complex subunit SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.350.10:FF:000006">
        <property type="entry name" value="FACT complex subunit SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.230.10:FF:000005">
        <property type="entry name" value="FACT complex subunit spt16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.210">
        <property type="entry name" value="FACT complex subunit Spt16p/Cdc68p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029148">
        <property type="entry name" value="FACT-SPT16_Nlobe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056595">
        <property type="entry name" value="Fact-SPT16_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048969">
        <property type="entry name" value="FACT_SPT16_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013953">
        <property type="entry name" value="FACT_SPT16_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013719">
        <property type="entry name" value="RTT106/SPT16-like_middle_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040258">
        <property type="entry name" value="Spt16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033825">
        <property type="entry name" value="Spt16_M24"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13980">
        <property type="entry name" value="CDC68 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13980:SF15">
        <property type="entry name" value="FACT COMPLEX SUBUNIT SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14826">
        <property type="entry name" value="FACT-Spt16_Nlob"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24824">
        <property type="entry name" value="PH_SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08512">
        <property type="entry name" value="Rttp106-like_middle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08644">
        <property type="entry name" value="SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21091">
        <property type="entry name" value="SPT16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01285">
        <property type="entry name" value="FACT-Spt16_Nlob"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01287">
        <property type="entry name" value="Rtt106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01286">
        <property type="entry name" value="SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 10" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8 10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 10" id="KW-0235">DNA replication</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="FACT complex subunit SPT16 N-terminal lobe" evidence="13">
        <location>
            <begin position="6"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="FACT complex subunit SPT16 middle" evidence="14">
        <location>
            <begin position="552"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="domain" description="Histone chaperone RTT106/FACT complex subunit SPT16-like middle" evidence="15">
        <location>
            <begin position="832"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="454"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="956"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="634"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="480"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="956"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1017"/>
            <end position="1032"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU367052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM01287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAR24686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1032" mass="117227" checksum="181863A992485FD6" modified="2009-07-28" version="1">MSDVEINFKVFQKRLTQLHSEFRNFEGSPQSLLFVVGSSDEEDPYKKSTILHNWLLGYEFPATLIAFVPDKVIIVTGAGKAKHLLGAVDLFKDSAFKLELWQRKAKDDAHNDKLFADVIELLKTAGKTVGTPVSDKYQGKFIAQWTPHWEGATGKGEFETVDIALGLSQVWEKKDDQEVAFLKVSSKGTDLFMNLLSDEMVTAVDEELKISNAKLSDKIENKIDDPKFLKKLANQMAPLCPSGEKFDINLLDWAYSPIIQSGSKFDLKVSARSNNDQLHGNGCILASCGIRYKSYCSNVTRTFLIDPTEEMVSNYEFLVSLQQEIINSFLKIGKTPKEVYESTVEYVKSHKPHLLSGLTKNVGSLIGLEFRDSQFVLNSKNEQRKIGEQDCFNVSLGFNNLKDSKTGVNYAVQIADTVLISSDGKAAALTDYTKLKSQISFYFNNEEEEVTTKVKKENAAGSPPIPMKSDGNSKILRSKLRGESRVQEDSQKEQIRKENQRKLHEKLQKNGLIRFSAADANGADDQPQHQFKKYESYIRETQIPNNVRDLRVHVDWKNQTIILPIYGRPVPFHINCYKNGSKNEEGEYTYLRLNFHSPGAGGISKKAEELPYEDSPDYQFVRSISLRSKDGDRMNEVFKQIADLKKEATKREQERKLLADVVEQAKLLENKSGRTKRLDQIFARPSPDAKRVPGTVFIHENGIRYQSPLRTDSRIDILFSNIKNLIFQPCKGELIVIIHVHLKNPILMGKKKIQDVQFYREASDMAVDETGNGRRSNMKFRRYGDEDELEQEQEERRKRAALDKEFRYFAEAIADASDGLVDLDTTFRELGFQGVPSRSAVYCMPTRDCLVQLVEPPFLVINLSEVEICVFERVQFGLKNFDMVFVYKDFSKPVTHINTVPIEDLELLKSWLTDVDIPYAVSTINLKWSTIMKSMQEDPHQFFLEGGWSFLVAGSDDEMSDESEEEISEYEASDEDPSDESAYSEEDGGSEDDFSDGGSEDASADESEEEGEDWDELEKKAAKADRENRQNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DM83</accession>
    <name>C5DM83_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0G06754p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0G06754g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555331.1">
        <property type="nucleotide sequence ID" value="XM_002555285.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DM83"/>
    <dbReference type="FunCoup" id="C5DM83">
        <property type="interactions" value="43"/>
    </dbReference>
    <dbReference type="GeneID" id="8293600"/>
    <dbReference type="KEGG" id="lth:KLTH0G06754g"/>
    <dbReference type="eggNOG" id="KOG0255">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008455_11_5_1"/>
    <dbReference type="InParanoid" id="C5DM83"/>
    <dbReference type="OMA" id="MYHKLGD"/>
    <dbReference type="OrthoDB" id="9986881at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140115">
        <property type="term" value="P:export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042908">
        <property type="term" value="P:xenobiotic transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17323">
        <property type="entry name" value="MFS_Tpo1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000082">
        <property type="entry name" value="MFS multidrug transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF7">
        <property type="entry name" value="DRUG_PROTON ANTIPORTER YHK8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="40"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="133"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="277"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="311"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="352"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="379"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="410"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="445"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="38"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR24894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="480" mass="53442" checksum="67DD859243CFB11D" modified="2009-07-28" version="1">MTSVSESELETEAKKEQFEVVLEPDDPENVETQMRNANKWFVALLVTVTSADITMTSSCWSLAQADIRRRFHISRELSVLGLSLYIFGMGVGPLLLSPLSELYGRKPTFISSLSLSICFLFLTAWGPNIGAVFVGRFLSGFFGSAFLSVAGGVISDMFSKHQIGIPMTIYTTAAFTGPAIGPLISGALSKHSYRWTFVVMIIESALFLALFVVAFKETYKPVLLLRKAKRLREETGDDRYCAPLERIRRETSLPKAILLSASRPFGLLTRDPMMGVLCFYTGLTLAIIYMFFIAFPLVFGSLYSFTTMELGVSYMGMLVGIVLVAPTSLWFQRRYAQKVINSGGQQVPELRFEPLFYGAFLTPIGLMIFAWTSYSHVHWIAPIIGTAVFGAGVFFVFTGVFNYTVDAFRLYSASAMACNSFVRSMMAGVFPLFTTQMYEGMGVNWASFLLAMVSIAMIPVPFLFTRYGAYLRSKSPYAWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMH3</accession>
    <name>C5DMH3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">lysine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0G08954g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.1.1.6" evidence="1"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555421.1">
        <property type="nucleotide sequence ID" value="XM_002555375.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMH3"/>
    <dbReference type="FunCoup" id="C5DMH3">
        <property type="interactions" value="110"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMH3"/>
    <dbReference type="GeneID" id="8294193"/>
    <dbReference type="KEGG" id="lth:KLTH0G08954g"/>
    <dbReference type="eggNOG" id="KOG1885">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008255_6_0_1"/>
    <dbReference type="InParanoid" id="C5DMH3"/>
    <dbReference type="OMA" id="MKWGMPP"/>
    <dbReference type="OrthoDB" id="21243at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004824">
        <property type="term" value="F:lysine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070154">
        <property type="term" value="P:mitochondrial lysyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002313">
        <property type="entry name" value="Lys-tRNA-ligase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018149">
        <property type="entry name" value="Lys-tRNA-synth_II_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00499">
        <property type="entry name" value="lysS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42918:SF5">
        <property type="entry name" value="LYSINE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42918">
        <property type="entry name" value="LYSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00982">
        <property type="entry name" value="TRNASYNTHLYS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="6">
        <location>
            <begin position="192"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR24984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="524" mass="58071" checksum="063D9AB6E303CB43" modified="2009-07-28" version="1">MLRPASPLARSLARGLSTALDFARRNEAIAARARDFYPSLSRVAPATATVAGFVRKYCDLEHDRPDLREVLRARVDSVRVAGKNMCFVDVSSARCSTQLILNYNVMAAHAAPGAAALSRQQFFQHVQDLRPGDHVQACGFPGRSQRERTLSLKCTQLTTLLAPALRALPPRLSDPAKRNQNRVLDYLVNGHDALRVRHSVLSALREFLDARGFLEAETPVLSPKANGAAAEPFTTRTNATNAALQLRVAPELWLKRLVVAGVDRVYELGRVFRNEGVDASHNPEFTTLEFYQTYASMEDLIALAEQLLLHVLERADTARARELKAVLLANGGRFARVEFLPTLQRETGIDFSQLDLADPRALAAALEARGIELDTADRSPQQILNALCARYIEDRHCRGSLPTLIFHHPTVMSPLAKGNPDDDMLTTKRFEVFIGGKEYINAYEEENCPQAQLQKFEAQQQAQEAYGDRESLAIDHAYVDAMKWGMPPIGGFGVGIDRLCMLLTGDTRIERVLSFGTIDDIGKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5DML0</accession>
    <name>C5DML0_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Conserved oligomeric Golgi complex subunit 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0G09790g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555458.1">
        <property type="nucleotide sequence ID" value="XM_002555412.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DML0"/>
    <dbReference type="FunCoup" id="C5DML0">
        <property type="interactions" value="55"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DML0"/>
    <dbReference type="GeneID" id="8293736"/>
    <dbReference type="KEGG" id="lth:KLTH0G09790g"/>
    <dbReference type="eggNOG" id="ENOG502QQP3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060138_0_0_1"/>
    <dbReference type="InParanoid" id="C5DML0"/>
    <dbReference type="OMA" id="YFWRTLA"/>
    <dbReference type="OrthoDB" id="18786at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017119">
        <property type="term" value="C:Golgi transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019465">
        <property type="entry name" value="Cog5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048485">
        <property type="entry name" value="COG5_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049176">
        <property type="entry name" value="COG5_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13228">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX COMPONENT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13228:SF3">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20649">
        <property type="entry name" value="COG5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10392">
        <property type="entry name" value="COG5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Conserved oligomeric Golgi complex subunit 5 N-terminal" evidence="5">
        <location>
            <begin position="9"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="Conserved oligomeric Golgi complex subunit 5 helical" evidence="6">
        <location>
            <begin position="180"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF10392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF20649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR25021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="387" mass="43450" checksum="1BBDE120AB1EA506" modified="2009-07-28" version="1">MSDPLAAFEDYLTPDFNASRTCNKLLKASNPDLQCEELDLVTPLKKVKYHIEEIERRNDDVIKANPSHLIESFDSKKIAQVQTRDALSSSLEYLSMSYKRLDAEVLEPYQKCLLLRSALSKIHQTSSILRDVSIFLHLLRQVTGFGTLDQTDPSAHQKALILASIHSQIQTELSSNPNLGALELVKKYDVETILPSRRNTVRYLSDRLFSDCANNLEVQSKNLDVTQLAKALYELSQKDFITVIDRVLLSKITNSTQTLSKTITSIKTLPAALETVMKNGRDVRALGRALNEAASTKSTLLKDYLSHKKHSCISEMFWSRVSKNFKREFEISFNRGGPVGKSLAANSSVILESIINAMKDSTEKENGESNLEKMLDSVAILNLHTTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMR8</accession>
    <name>C5DMR8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Mediator of RNA polymerase II transcription subunit 5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">Mediator complex subunit 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MED5</name>
        <name evidence="10" type="ordered locus">KLTH0G11110g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the Mediator complex subunit 5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMR8"/>
    <dbReference type="FunCoup" id="C5DMR8">
        <property type="interactions" value="246"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMR8"/>
    <dbReference type="KEGG" id="lth:KLTH0G11110g"/>
    <dbReference type="eggNOG" id="ENOG502R1HB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_281615_0_0_1"/>
    <dbReference type="InParanoid" id="C5DMR8"/>
    <dbReference type="OMA" id="FTCFAQF"/>
    <dbReference type="OrthoDB" id="5322661at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014801">
        <property type="entry name" value="Mediator_Med5_fun"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35784">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35784:SF1">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08689">
        <property type="entry name" value="Med5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0010">Activator</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR25079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1075" mass="120619" checksum="5618396271E3F6BC" modified="2009-07-28" version="1">MDENQDPIYSLALNCSRRRLPSNLFVNFYNELVNEKYGSMINSGEETDADIKQTNVCEKISSSLLRLLDAEKHFLIVDYVVEVLFINYNKELVKELLPQLYSIKDSSQLVLFLSKASAFFLRLTDKLVIDQICGDLGNVIVPSILSSSFNTQGEELIVAIAKFLRNVLSLSSNPIAISDPKVGDRVRELLNCLSKINRLLYKKVAGDFDSLLRLGGSAPPTAREERHGVSSPSIISPRYLESPINVTKQPQSLIPSSVNHQTVKLARYCKNLWLNSRIMGWRTNDVAFVTKYSKIEAEIMKKTSPSQLNVEAAMKDLIETAFTSFAQFVSNKQYHQANSAFNLLERQWNIFITKQLPLHIMENASDMPHIVINALENIDHKVVKALKSYASDKDDAKNRGEDLFDDLPNKNTDIRHDFLKSLIMIGLQPAAVLNDFLREDQVVDTKSLPTNDTVVIKNAQGVKETVDNFSQFIKDTIEDLDAESMFEATDGSLGNADNGIMQILRKFETLAATKQKDLANVFYDLFRESVSSFDCKTFSKTCCLMCFNMSHSLTTMFAFVNPQKFLAVAIEFVDQIWESSVRKASAGLNDESDFEPNTEFVCFTYALIFIVQVVGTYNVSLIEALSTSSSDPQSSFIVNYITKLGDIAPALKLNSTLDSSEVLKSWVRDLFINGSISDSLMKSANVKDLAVLIPFIFKESLSAVESGSIRDISALVGGFEYFLQPFLIVGLIGIVFWTEQYLTTLRSKDLAKDLLESIFDMLNSVLNPSTLNDESRPLHTLILRLNTVQLLKATRAFQTQTQSSYGTYSSDSEGSTKVDSLISHLEQVARSSILYNVDSSVINSEIGYQRKDINYTPFAITADNSINSILTNQVNSFWNLHSSTYYNIDYLFALIDIITPQRFLEDMLVVLQHKTTDAALPNSRTKGSDGENLFYLDYFFYFLVSHDVMKAENKTDILQYMSSTAESDLIFRKPAHEFQPSAKVEQPSDEDFDMLFGEDTSIPGNESEANINEIKADEISPINPEFLRCSFAAVLHCSLLANKAALESGHTSEVAVDQLTQLHDKYIEILKSSTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMS3</accession>
    <name>C5DMS3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0G11220p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0G11220g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555521.1">
        <property type="nucleotide sequence ID" value="XM_002555475.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMS3"/>
    <dbReference type="FunCoup" id="C5DMS3">
        <property type="interactions" value="71"/>
    </dbReference>
    <dbReference type="GeneID" id="8293800"/>
    <dbReference type="KEGG" id="lth:KLTH0G11220g"/>
    <dbReference type="eggNOG" id="ENOG502QU6H">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021924_0_1_1"/>
    <dbReference type="InParanoid" id="C5DMS3"/>
    <dbReference type="OMA" id="GVAYCCI"/>
    <dbReference type="OrthoDB" id="435607at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040254">
        <property type="entry name" value="Ecm3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004776">
        <property type="entry name" value="Mem_transp_PIN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31274:SF1">
        <property type="entry name" value="AGL149CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31274">
        <property type="entry name" value="PROTEIN ECM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03547">
        <property type="entry name" value="Mem_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="103"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="141"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="430"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="522"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="553"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="589"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="175"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="366"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="180"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="192"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="264"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="312"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR25084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="619" mass="68012" checksum="16BBCACB27E5D30F" modified="2009-07-28" version="1">MSIGVGVAIYAAIKPVLKIYVIILVGYLLARYNLATVETSRGVSNMVVNAILPCLTFNKIVGNISDKDIKEVGVLVLTAFLIFAVGGVGALITKWLTNSPKQWYWGLLFAGIFPNISDLPIAYVQSMSNGSVFTSGQVDRGVAYCCIFLCTQSFLMMNFGMFRLVGLDFREPSKDEEQLSENSDNSSHLNSKLREGEFTKTPENDVIADKQGPASFHGDRQPQMLASDKGSVDMPRSLYGQDENSSQSEADLGDEPQHFLNTVSTGSSLSSSSGATASDVFRSGSRHSGSENSHSRAGVPISISDTIHRMTTARSTASALSIGSQRKSRGRQRRLSQTAKDVINEYSAVQKIKTGEMDLSRPLSLTQEFGSGNAFPSDKSDDEDEEYDGSLPAEGDRRASYTSSSQLSRRRSKKWKRSLASFVERYKLGWLVYIMVNFCRPASLGALLGIIFCMIPWVKALFVHTDVHMHQAPDGQPVLNFLMDFTAYIGNACVPLGLLLLGGTLARLEIGSLPKGFWKTTVAMTAFRLVILPIVGIAWANKLYDINWIENDVAKFVVILTWAMPSATAQVYFTAFYTPLEGEHLQMDCLSVFFLSQYAVLFITLSIVVSYALKVDLKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>C5DN87</accession>
    <name>C5DN87_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">40S ribosomal protein S30</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0G15004g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS30 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555685.1">
        <property type="nucleotide sequence ID" value="XM_002555639.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DN87"/>
    <dbReference type="FunCoup" id="C5DN87">
        <property type="interactions" value="463"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DN87"/>
    <dbReference type="GeneID" id="8293970"/>
    <dbReference type="KEGG" id="lth:KLTH0G15004g"/>
    <dbReference type="eggNOG" id="KOG0009">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010412_5_1_1"/>
    <dbReference type="InParanoid" id="C5DN87"/>
    <dbReference type="OMA" id="YNTQNVP"/>
    <dbReference type="OrthoDB" id="199599at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006846">
        <property type="entry name" value="Ribosomal_eS30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12650">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S30/UBIQUITIN-LIKE PROTEIN FUBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12650:SF15">
        <property type="entry name" value="RIBOSOMAL PROTEIN S30, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04758">
        <property type="entry name" value="Ribosomal_S30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR25248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="62" mass="6977" checksum="76695DC66C30CC0E" modified="2009-07-28" version="1">MAKVHGSLARAGKVKSQTPKVEKQEKPKLPKGRAKKRMLYTRRFVNVTLTNGKRKMNPSPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNM0</accession>
    <name>C5DNM0_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8">Eukaryotic translation initiation factor 2A</fullName>
            <shortName evidence="8">eIF-2A</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0G18238g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40S subunits in a GTP-dependent manner.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the WD repeat EIF2A family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555818.1">
        <property type="nucleotide sequence ID" value="XM_002555772.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNM0"/>
    <dbReference type="FunCoup" id="C5DNM0">
        <property type="interactions" value="1279"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNM0"/>
    <dbReference type="GeneID" id="8294113"/>
    <dbReference type="KEGG" id="lth:KLTH0G18238g"/>
    <dbReference type="eggNOG" id="KOG2315">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013809_0_1_1"/>
    <dbReference type="InParanoid" id="C5DNM0"/>
    <dbReference type="OMA" id="RCCAYSP"/>
    <dbReference type="OrthoDB" id="2194683at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000596">
        <property type="entry name" value="Eukaryotic translation initiation factor 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011387">
        <property type="entry name" value="TIF2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013979">
        <property type="entry name" value="TIF_beta_prop-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13227">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13227:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08662">
        <property type="entry name" value="eIF2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017222">
        <property type="entry name" value="eIF2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="7 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 8" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Translation initiation factor beta propellor-like" evidence="10">
        <location>
            <begin position="217"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="461"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="482"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="546"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="563"/>
            <end position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR017222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR25381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="626" mass="69332" checksum="5D6561E35F17BDD0" modified="2009-07-28" version="1">MSTQLFAKTSTEINLFQGYPSFDELTRDEAETIQVSLLSPCGRFMALATADAVQIFTGMEFSNRLLRVALKDVYDMQFSPSGNFLSTWERPSIAEPDHKNVKIWDLNVETPPEVPRYAYQAKVQSGWVLQFSKLDNFALKQFGKEIRIVKLDGEESTFNFDKPYSKLVPEQPISSFLISPAEFPTICTFSPEKSGKPAVLSIYAIAEGVTDKKIASKTFFKADSCQLKWNQQGNAVLCVAITDFDSSNKSYYGENTLYLLSFQGVNGALGGKSVRVPLSKEGPIHDFTWSPTSRQFGVIYGYMPATITFFDLRGNVVHSLPEQPKNTMLFSPSGRYILIAGFGNLQGSVEVLDRHDKFNCISKFDAANTSVCKWSPGGEFILTATTSPRLRVDNGLKIWHVSGTLCFVKEYKELLKIDWRHKVSQNLEKSHVIAKNEDPASDNFSDPKLGKIEVKIHPSVLEYNAKRQKNGSSDGAKDKPTGAYRPPHARRAGGAASVPGAAVKPSQRVIPGMAPPPGAVPGVAKESKAAAKNKKKRESKKKETTPDATQPDADSQAANQPRVNKEASPEEKKIRSLLKKLRAIESLKQKLAAGESLEDTQLLKIQTEDKVCKELEFLGWKNEDAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNT3</accession>
    <name>C5DNT3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0G19646p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0G19646g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555881.1">
        <property type="nucleotide sequence ID" value="XM_002555835.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNT3"/>
    <dbReference type="STRING" id="559295.C5DNT3"/>
    <dbReference type="GeneID" id="8294177"/>
    <dbReference type="KEGG" id="lth:KLTH0G19646g"/>
    <dbReference type="eggNOG" id="KOG0259">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_4_4_1"/>
    <dbReference type="InParanoid" id="C5DNT3"/>
    <dbReference type="OMA" id="IMAARDY"/>
    <dbReference type="OrthoDB" id="7042322at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43510">
        <property type="entry name" value="AMINOTRANSFERASE FUNCTION, HYPOTHETICAL (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43510:SF1">
        <property type="entry name" value="AMINOTRANSFERASE FUNCTION, HYPOTHETICAL (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="1">
        <location>
            <begin position="48"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR25444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="379" mass="42324" checksum="AAFCF076909C282F" modified="2009-07-28" version="1">MPFQEDFLIEQFMDKHENDIKYNMGETCCYSLNLNELSSLTGESFEFDQNMRLTYGSIWGTPVLRDLVAKIHSSDEIQLTADNVLITNGAIGANFLVHYTAAGPGDHIICVDPTYQQLSSVPSMFGADVELLHLEAEDGYTPNIQKLKSKVRANTKYIVLNNPNNPLGSVISTEKMTEIIDYAERNEIFIVCDEVYSPLFHSSEQPKSACQLSNRCIVTGSMSKAYSAAGVRLGWIVSRDKDFLKQAASRRDFNTISVSIIDDYISQYILKHRNAVLKRNFDLCRKNLMIMKDFINSSNGKFSFVHEPEGGSVCLLRIAGVDDTDSFAVKLATEFKVLCAPGECFGVPGTLRIGYGNSTEDLVEGLKILKLAYDKTYTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>C5E1X0</accession>
    <name>C5E1X0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">KLTH0H00308p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0H00308g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555893.1">
        <property type="nucleotide sequence ID" value="XM_002555847.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E1X0"/>
    <dbReference type="GeneID" id="8294204"/>
    <dbReference type="KEGG" id="lth:KLTH0H00308g"/>
    <dbReference type="eggNOG" id="KOG0254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_30_1_1"/>
    <dbReference type="InParanoid" id="C5E1X0"/>
    <dbReference type="OMA" id="PRWLYKM"/>
    <dbReference type="OrthoDB" id="6612291at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005536">
        <property type="term" value="F:D-glucose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010255">
        <property type="term" value="P:glucose mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17356">
        <property type="entry name" value="MFS_HXT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000115">
        <property type="entry name" value="High-affinity glucose transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF17">
        <property type="entry name" value="HEXOSE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="21"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="120"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="153"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="187"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="276"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="310"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="339"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="371"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="406"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="441"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="11">
        <location>
            <begin position="24"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR30031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="510" mass="55310" checksum="8C4B4B0DDE8B5268" modified="2009-07-28" version="1">MGFLQKVFLAEEDIQGSKFRAIFIGLFVAFGGVLFGYDTGTISGILAMKYVKEHFTHRGSFTAGETSLITSILSAGTFVGAISAPLVSDTMGRRFGLIASTIVFSIGVILQVAATGQDLLIVGRVIAGLGVGILSAIVPLYQSEAAPKWIRGAVVSCYQGAITVGLLLAACVNQGTHARTDSGSYRIPIAIQFAWALILFVGMIVLPESPRFYVKQGKIEKAGNALSRLRGLPADHPIVEGELEEILANFNYEKSLGSTTVWDCFKRGNHQLKRMLIGLAIQALQQLTGINFIFYYGTQFFQNSGIKNPFIIQVIMNVVNVVMTWPGVLLVELAGRRNLLLWGAVGMCVSELIVASVGTALPDSTAANKTLIAFSCTFIASFAATWGPLAWVVVGEIYPLRVRGKSVALCAASNWLFNFAIAYATPYLVDQEHANLQSKVFFIWGGCTFLCFLFVYFFIYETKGLTLEQIDELFETCTSAPDSKNFIPSSGYAHESPDAEKAVVEAVEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5E1Y2</accession>
    <name>C5E1Y2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">60S ribosomal export protein NMD3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0H00594g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Acts as an adapter for the XPO1/CRM1-mediated export of the 60S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the NMD3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555905.1">
        <property type="nucleotide sequence ID" value="XM_002555859.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E1Y2"/>
    <dbReference type="FunCoup" id="C5E1Y2">
        <property type="interactions" value="1449"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E1Y2"/>
    <dbReference type="GeneID" id="8294217"/>
    <dbReference type="KEGG" id="lth:KLTH0H00594g"/>
    <dbReference type="eggNOG" id="KOG2613">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027444_2_0_1"/>
    <dbReference type="InParanoid" id="C5E1Y2"/>
    <dbReference type="OMA" id="VILVRKH"/>
    <dbReference type="OrthoDB" id="203821at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039768">
        <property type="entry name" value="Nmd3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007064">
        <property type="entry name" value="Nmd3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048899">
        <property type="entry name" value="NMD_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048898">
        <property type="entry name" value="OB_NMD3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12746:SF2">
        <property type="entry name" value="60S RIBOSOMAL EXPORT PROTEIN NMD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12746">
        <property type="entry name" value="NONSENSE-MEDIATED MRNA DECAY PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04981">
        <property type="entry name" value="NMD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21193">
        <property type="entry name" value="NMD_SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21192">
        <property type="entry name" value="OB_NMD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Nmd3 N-terminal" evidence="10">
        <location>
            <begin position="21"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="60S ribosomal export protein NMD3 SH3" evidence="12">
        <location>
            <begin position="253"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="60S ribosomal export protein NMD3 OB-fold" evidence="11">
        <location>
            <begin position="317"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="463"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="434"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="478"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF21192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF21193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR30043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="512" mass="58286" checksum="03629E3145CDADD6" modified="2009-07-28" version="1">MNYTPLDQSAFNNHQAATVLCCNCGTPMDGSSGLVMCYDCIKLTVDITEGIPRESNVSFCRNCERFLQPPGQWVRAELESRELLALCLRRLKGLNKVRLVDASFIWTEPHSRRIRVKLTVQGEAMANTIIQQTFEVEYVVVAMQCADCARSYTTNTWRATVQIRQKVSHKRTFLYLEQLILKHNAHVDTINIQEAKDGLDFFYAQKNHAVKMLDFLNAVVPIKSKKSEELVSQDTHTGASTYKFSYSVEIVPLCRDDLVVLPKKLAKSLGNISQFVLCSKISNTIQFLDPMTLQTSDLSPSVYWRSPFDSLADVSQLVEFIVLDVDPTGQTKGKWVLADITVARASDLGSNDQVYYVRSHLGAICHPGDSVMGYFIANSNYNSDLFDALNMEHVPDVVLVKKQYIRKTRKNRSWKLKRMAREHKDIEASQDYSSRQQKQEMERAEKDYELFLQELEEDAEMRQAVNLYKSAQQAPQDGEMDEDEEEDAPQIDIDELLDELDEMTLDEGAPPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>C5E1Y5</accession>
    <name>C5E1Y5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0H00660p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0H00660g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555908.1">
        <property type="nucleotide sequence ID" value="XM_002555862.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E1Y5"/>
    <dbReference type="FunCoup" id="C5E1Y5">
        <property type="interactions" value="538"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E1Y5"/>
    <dbReference type="GeneID" id="8294220"/>
    <dbReference type="KEGG" id="lth:KLTH0H00660g"/>
    <dbReference type="eggNOG" id="KOG1489">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011747_2_6_1"/>
    <dbReference type="InParanoid" id="C5E1Y5"/>
    <dbReference type="OMA" id="PRVGHWE"/>
    <dbReference type="OrthoDB" id="347018at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01898">
        <property type="entry name" value="Obg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.210.12:FF:000001">
        <property type="entry name" value="GTPase Obg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.210.12">
        <property type="entry name" value="GTP1/OBG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014100">
        <property type="entry name" value="GTP-bd_Obg/CgtA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006169">
        <property type="entry name" value="GTP1_OBG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036726">
        <property type="entry name" value="GTP1_OBG_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045086">
        <property type="entry name" value="OBG_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11702">
        <property type="entry name" value="DEVELOPMENTALLY REGULATED GTP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11702:SF31">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOME-ASSOCIATED GTPASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01018">
        <property type="entry name" value="GTP1_OBG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002401">
        <property type="entry name" value="GTP_bd_Obg/CgtA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82051">
        <property type="entry name" value="Obg GTP-binding protein N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51883">
        <property type="entry name" value="OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Obg" evidence="6">
        <location>
            <begin position="104"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="OBG-type G" evidence="5">
        <location>
            <begin position="360"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR30046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="536" mass="59127" checksum="7587F6B7E55DF1F2" modified="2009-07-28" version="1">MVLRRPIQRASLRVRFNSTAPDNAPKASDNEIWLRKLSNNRKLAHAPEVSQKIQPASSSQDHRNRFEIISTPPDTSFIGVKAPLSEFTSLSHIQSNNQRHAQAKKFVDVRILKCRSGQGGDGAVSFFRDAGRAIGPPDGGDGGDGGSVFVQAIEGLNSLAKLKTSYIAEDGANGAAKQLDGARGKDVLISVPVGTVVKWCLNPSAVRSFIEKFKNRGTVNLRQLLEQNKVSLQCTGRFEMDQKPSFIQLFRESYEAGEGWHFKGKDEEYHLEKNWFKQLKEKVSIYDLELASSELSADKFPLLGIDLDKPSDRPICLVKGGKGGLGNMHFLTNLIRNPRFSKMGRSGLEQFFMFELKSLADIGLVGLPNAGKSTILNKISKARPKIGHWEFTTTHPSIGTFNVGAEGFGFTVADIPGIIKDASKDKGMGLEFLRHIQRSKGWTIVVSLEREDPLADLQLLISELGGMEEVARKNVLVVCNKADIEPHNPQSLDKFLDVQGFCETQNWDIIPISALKGENIDLLIEKMARCAETSAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5E1Z6</accession>
    <name>C5E1Z6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein SDA1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0H00902g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Required for 60S pre-ribosomal subunits export to the cytoplasm.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
            <location evidence="6">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the SDA1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555919.1">
        <property type="nucleotide sequence ID" value="XM_002555873.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E1Z6"/>
    <dbReference type="FunCoup" id="C5E1Z6">
        <property type="interactions" value="1203"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E1Z6"/>
    <dbReference type="GeneID" id="8294231"/>
    <dbReference type="KEGG" id="lth:KLTH0H00902g"/>
    <dbReference type="eggNOG" id="KOG2229">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009161_2_1_1"/>
    <dbReference type="InParanoid" id="C5E1Z6"/>
    <dbReference type="OMA" id="AMYKTYK"/>
    <dbReference type="OrthoDB" id="2196187at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027312">
        <property type="entry name" value="Sda1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048292">
        <property type="entry name" value="SDA1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007949">
        <property type="entry name" value="SDA1_MD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012977">
        <property type="entry name" value="SDA1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12730">
        <property type="entry name" value="HSDA/SDA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12730:SF0">
        <property type="entry name" value="PROTEIN SDA1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21638">
        <property type="entry name" value="SDA1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05285">
        <property type="entry name" value="SDA1_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08158">
        <property type="entry name" value="SDA1_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4 6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="2 6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SDA1 N-terminal" evidence="9">
        <location>
            <begin position="70"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="SDA1 middle" evidence="8">
        <location>
            <begin position="552"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="domain" description="SDA1 C-terminal" evidence="10">
        <location>
            <begin position="681"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="475"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="522"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="638"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="487"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="524"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="563"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="652"/>
            <end position="687"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF05285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF21638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR30057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="730" mass="82586" checksum="54B125088C4F54FF" modified="2009-07-28" version="1">MVRKSRAAILPTNVILLQNLVKRDPQSYREEFLQQYSHYESLRDIFMLNAMENQVADDSNTSQFVELVGFVSQVCSCFPEETANFPNELRQLVLEYHKSLPFEVREKIITSLTMLRNKGVISAETLIQTMFPLLIAYSSHSNALGPNSHAKEVRKLVYSTLISLLKNCNTGSKNQKLNKSTQAICFNLLEQPDSQGVWASRLTRELWRRGIWDDSRAVEIMTQAALHDDVKIAYSGVLFFLGADREREENFEDNSDDEDIDVDALKHKMQINKKSGKRGKKLEAAVKSVKKKKAGAKTPQGFLNFSAIHLLRDPQGFADKLFTKQLSSKNNNRFDIDQKIALMQLLSRLIGTHKLMVLGIYSFFLKYLTPKQRDVTKIMASAAQACHDLVPPETISVMVRKIADEFVSDGVASEVAAAGLNTIREICSRAPLAIDETLLQDLTEYKGSKAKGVSIAAKSLVSLYREVAPEMLRRKDRGKVASMEMQDASRKGKESRRPKFGEESHVQGIAGIELLAQWKKDNEKVDDEEGDDKNWEVAEDSDSDADDDDGEWVTMESDKEYDVDMGDSDDEDAKAAKKAEAAEEDEPAAPAEKIDAETAFRDLASTRVLTPADFAKLQELRTEQGVARLMGVRNEETVDSDRLVGPVKHKNTKEERLQSVLEGREGREKFGSRKKSRGTEHSTTNREKQRKKNFVMMIHKRSVKGKQKMSLRDRQKVLRQHITKQKKRGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>C5E283</accession>
    <name>C5E283_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 10">Signal recognition particle subunit SRP68</fullName>
            <shortName evidence="10">SRP68</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0H02882g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the signal recognition particle (SRP) complex, a ribonucleoprotein complex that mediates the cotranslational targeting of secretory and membrane proteins to the endoplasmic reticulum (ER). The SRP complex interacts with the signal sequence in nascent secretory and membrane proteins and directs them to the membrane of the ER.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the SRP68 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556006.1">
        <property type="nucleotide sequence ID" value="XM_002555960.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E283"/>
    <dbReference type="FunCoup" id="C5E283">
        <property type="interactions" value="1007"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E283"/>
    <dbReference type="GeneID" id="8294319"/>
    <dbReference type="KEGG" id="lth:KLTH0H02882g"/>
    <dbReference type="eggNOG" id="KOG2460">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018649_2_1_1"/>
    <dbReference type="InParanoid" id="C5E283"/>
    <dbReference type="OMA" id="LAYIKYN"/>
    <dbReference type="OrthoDB" id="10255118at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005786">
        <property type="term" value="C:signal recognition particle, endoplasmic reticulum targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030942">
        <property type="term" value="F:endoplasmic reticulum signal peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005047">
        <property type="term" value="F:signal recognition particle binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15481">
        <property type="entry name" value="SRP68-RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3450.40">
        <property type="entry name" value="Signal recognition particle, SRP68 subunit, RNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026258">
        <property type="entry name" value="SRP68"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034652">
        <property type="entry name" value="SRP68-RBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038253">
        <property type="entry name" value="SRP68_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12860">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 68 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12860:SF0">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE SUBUNIT SRP68"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16969">
        <property type="entry name" value="SRP68"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038995">
        <property type="entry name" value="SRP68"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 10" id="KW-0733">Signal recognition particle</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="548"/>
            <end position="585"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR038995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR30144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="585" mass="66475" checksum="54E7D5629E331FC5" modified="2009-07-28" version="1">MSYYSPLGATFGVRIDRFLETAEDFDKYHQKLNRKLQKLRHHCRLTTKDTKRYSTKEKYSKITSDDYDKNKLFGTLMLLHAERDLALVESLKLKARLRGAMKKSEKKVVMTRLRKACKTSEQLTVLTQNEGDDFTRAEYAIYHKLCIVSYFSMCKPSKTRYTNKIAKELSLAIASLHYLQSKEKVSDEVASSIISQFEYLLRQHADAEVFTTSEVRNFIAKQVRQSSDDVLVQLITRSGYEAPIEDSVMGSEKSNLKEVQWRRFSAKVRDSKVAELISEATQIEPTDLAQFSNKLAKWQEAADAHSVFIAQKERDDEDEDVGEDDQILLTYIKYNSHFTTIQRDDIVFQKLWAQWSKSANKSKNLKLTKFKEIERIVHNLATYLQEVMDLPGVYSDDALMANLKLATTYYQLHLTAGCLASFYQSKQKYLEALALYIDGHKTLEVAAGEAVLQEPLPGNILTPEKVAALEDFIKVSWRGVISLAEYEKIASPGGKPSLEPSLIEKLGNPIVPRDVSLTNLFPTRPIVRPVGSKPTLFDLAFNYIGSDSQTPQNEPLIPEESRSVPKSNDAQEAPKKKGLFGLFGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2B6</accession>
    <name>C5E2B6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">rRNA methyltransferase 2, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0H03630g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556039.1">
        <property type="nucleotide sequence ID" value="XM_002555993.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2B6"/>
    <dbReference type="FunCoup" id="C5E2B6">
        <property type="interactions" value="31"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2B6"/>
    <dbReference type="GeneID" id="8294352"/>
    <dbReference type="KEGG" id="lth:KLTH0H03630g"/>
    <dbReference type="eggNOG" id="KOG4589">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009422_2_0_1"/>
    <dbReference type="InParanoid" id="C5E2B6"/>
    <dbReference type="OMA" id="WSQVAVN"/>
    <dbReference type="OrthoDB" id="20105at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008650">
        <property type="term" value="F:rRNA (uridine-2'-O-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01547">
        <property type="entry name" value="RNA_methyltr_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050082">
        <property type="entry name" value="RNA_methyltr_RlmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002877">
        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015507">
        <property type="entry name" value="rRNA-MeTfrase_E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920">
        <property type="entry name" value="RIBOSOMAL RNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920:SF18">
        <property type="entry name" value="RRNA METHYLTRANSFERASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01728">
        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005461">
        <property type="entry name" value="23S_rRNA_mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribosomal RNA methyltransferase FtsJ" evidence="8">
        <location>
            <begin position="54"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00041184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR005461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR30177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="310" mass="35223" checksum="B452C0D7F4CA4DB6" modified="2009-07-28" version="1">MTSPMLCALPKFLCQGQCLRWRVAVRFNSSAKSRWLDRQKGDSFTREAKVQGLRSRAAFKLMHLDDKYHLFRKGSSQKVLDLGYAPGAWSQVALDRTGPNGVILGVDILPCAPPRGVSAIQGNILSKKTHELIRLFFSKHFQLYQEDQLHKDNGYFQHMLEQELEGLIPDGSKTGLQIDRSKVEKTPIDVIVSDMYEPWPQTTGFWNNITNSAYDRMANTTGVAIKDHYMSMDLCDAALVTAIDLMKPGGSFVCKLYTGKEDKLLESRLKKAFENVHRFKPSASRNESKELYFIGLKKKHKVDKLKVFSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2F3</accession>
    <name>C5E2F3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Delta 8-(E)-sphingolipid desaturase</fullName>
            <ecNumber evidence="6">1.14.19.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ordered locus">KLTH0H04488g</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Delta(8)-fatty-acid desaturase which introduces a double bond at the 8-position in the long-chain base (LCB) of ceramides. Required for the formation of the di-unsaturated sphingoid base (E,E)-sphinga-4,8-dienine during glucosylceramide (GluCer) biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>an N-acylsphing-4-enine + 2 Fe(II)-[cytochrome b5] + O2 + 2 H(+) = a (4E,8E)-4-sphinga-4,8-dienine ceramide + 2 Fe(III)-[cytochrome b5] + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:46280"/>
            <dbReference type="Rhea" id="RHEA-COMP:10438"/>
            <dbReference type="Rhea" id="RHEA-COMP:10439"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:52639"/>
            <dbReference type="ChEBI" id="CHEBI:85953"/>
            <dbReference type="EC" id="1.14.19.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the fatty acid desaturase type 1 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.19.18" evidence="6"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556076.1">
        <property type="nucleotide sequence ID" value="XM_002556030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2F3"/>
    <dbReference type="STRING" id="559295.C5E2F3"/>
    <dbReference type="GeneID" id="8294389"/>
    <dbReference type="KEGG" id="lth:KLTH0H04488g"/>
    <dbReference type="eggNOG" id="KOG4232">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016265_3_1_1"/>
    <dbReference type="InParanoid" id="C5E2F3"/>
    <dbReference type="OMA" id="SYMFLRM"/>
    <dbReference type="OrthoDB" id="260091at2759"/>
    <dbReference type="UniPathway" id="UPA00222"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016717">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006665">
        <property type="term" value="P:sphingolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03506">
        <property type="entry name" value="Delta6-FADS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012171">
        <property type="entry name" value="Fatty_acid_desaturase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19353:SF30">
        <property type="entry name" value="DELTA 8-(E)-SPHINGOLIPID DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19353">
        <property type="entry name" value="FATTY ACID DESATURASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015921">
        <property type="entry name" value="FA_sphinglp_des"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0349">Heme</keyword>
    <keyword evidence="14" id="KW-0408">Iron</keyword>
    <keyword evidence="15" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="16 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="9 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="221"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="276"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="353"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="390"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="421"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="20">
        <location>
            <begin position="2"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="91"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="545"/>
            <end position="574"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00016939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="CAR30214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="574" mass="66748" checksum="043CCECE408E9863" modified="2009-07-28" version="1">MTTILERSEIEERICRGQAIVIYDGNVLRLDKWIKYHPGGDKAIHHMIGRDATDEMKAYHCDETVSTFLKWRIGRVSGRWHNFLPPIQGGVFKSREDQEGSGPSCEPESEWRDVDTSDAFQDISKTKRMCGEPDVKIYPKIPQGVVPTLDLKLAFEKKVVTDPQDITENYDNEQVKHDLLTLPSLDAETQDWLSQEYNEMHQEVIQAGLYKCNYFRYAKELLRIGALFGLSYFLLFHKNQKFWSAVSMGAAWQQLVFIAHDAGHIGITHNYQADNIIGMVIASWIGGLSLGWWKRNHNVHHLVTNDPIHDPDIQHLPFFAVSTRLFNNIYSTYYERFLWFDKFAQKVIPLQRFLYYPILAFGRFNLYRLSWMHVLLGQGPRRGKAAWFRYFELAGLIFFNYWFFYLIVICRLHTGWERFQYIMVSHITTMLVHVQITLSHFAMSTSDLGVAEGFPMRQLRTSMDVDCPRWLDFLHGGLQFQVVHHLFPRLPRHNLRDAQPFVIKFCEKVGLTYSIYGFKKGNGVVLGRLEEIAQQAKTMLKCAQTMEQEANSRPKPNISAPIDHTCSSKRHKLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2H1</accession>
    <name>C5E2H1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0H04906p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0H04906g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556094.1">
        <property type="nucleotide sequence ID" value="XM_002556048.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2H1"/>
    <dbReference type="FunCoup" id="C5E2H1">
        <property type="interactions" value="166"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2H1"/>
    <dbReference type="GeneID" id="8294408"/>
    <dbReference type="KEGG" id="lth:KLTH0H04906g"/>
    <dbReference type="eggNOG" id="KOG0118">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017390_1_0_1"/>
    <dbReference type="InParanoid" id="C5E2H1"/>
    <dbReference type="OMA" id="YGRDRCA"/>
    <dbReference type="OrthoDB" id="6407164at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051252">
        <property type="term" value="P:regulation of RNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12521">
        <property type="entry name" value="RRM3_MRN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000047">
        <property type="entry name" value="Differentiation 1 negative regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000064">
        <property type="entry name" value="Differentiation 1 negative regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000120">
        <property type="entry name" value="Negative regulator of differentiation 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000628">
        <property type="entry name" value="RNA binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039171">
        <property type="entry name" value="Cwc2/Slt11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14089">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR RBM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14089:SF8">
        <property type="entry name" value="RNA-BINDING PROTEIN MRN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="148"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="239"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="388"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="479"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="90"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="95"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR30232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="569" mass="62555" checksum="E7F5BD5C50A1BCA8" modified="2009-07-28" version="1">MSSHYHQQFMNTPMYAYDVPSAGLESIDDGLYRSLSPTSQVAYQNDRQAVWPNAGYQSNGAGYLEQHQMGYPPQSGILEQQPTFGVIPNETDNASSQISTGSSSYSSTPRNSKFVVAAAAAAAAANAPLSHKPLQLNNPRDFTSAPSRTVYLGNIPPNLEVRELLDHVRSGVVEDVRILREKMCAFISFLDESSALLFHSDAILKRLNIGGRDIKVGWGKPTPIDPVVTAAVSNDGATRNVYIGRLNTDRDLAPQFGADPRDLIATEEKLRADLDFYGEIESVKIIVEKGIAFVHFSSIFSAIKVVSSLATVNPYYSNKKIFYGKDRCAFVTKTQQHNAAQFLGLQPGMEHLVQYTDRDFISNTLLQQSAAAAAIATAAGGANNLGNRTIYLGNLPKDVKIEEICNAVRGGLLQSIKLLTDRHVCFVTFIDPTAAAQLYAMTSLHGLTIHNKRCKIGWGKHSGALSNALALAVSHGASRNIYLGNIDFKADSERDVPVFTEQNLRKLFEEYGEVEQINFLPERSCCFVNFTNISNAILAIDKIKSNEQFKDLKINFGKDRCGNVPRQYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2H3</accession>
    <name>C5E2H3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0H04950p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">NBP35</name>
        <name evidence="10" type="ordered locus">KLTH0H04950g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The NBP35-CFD1 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. Required for biogenesis and export of both ribosomal subunits, which may reflect a role in assembly of the Fe/S clusters in RLI1, a protein which performs rRNA processing and ribosome export.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the Mrp/NBP35 ATP-binding proteins family. NUBP1/NBP35 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556096.1">
        <property type="nucleotide sequence ID" value="XM_002556050.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2H3"/>
    <dbReference type="FunCoup" id="C5E2H3">
        <property type="interactions" value="612"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2H3"/>
    <dbReference type="GeneID" id="8294410"/>
    <dbReference type="KEGG" id="lth:KLTH0H04950g"/>
    <dbReference type="eggNOG" id="KOG3022">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024839_0_1_1"/>
    <dbReference type="InParanoid" id="C5E2H3"/>
    <dbReference type="OMA" id="VSGCPMR"/>
    <dbReference type="OrthoDB" id="1741334at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904564">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly scaffold complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140663">
        <property type="term" value="F:ATP-dependent FeS chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02037">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000427">
        <property type="entry name" value="Cytosolic Fe-S cluster assembly factor NUBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02040">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03038">
        <property type="entry name" value="NUBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000808">
        <property type="entry name" value="Mrp-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019591">
        <property type="entry name" value="Mrp/NBP35_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028601">
        <property type="entry name" value="NUBP1/Nbp35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033756">
        <property type="entry name" value="YlxH/NBP35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23264:SF35">
        <property type="entry name" value="CYTOSOLIC FE-S CLUSTER ASSEMBLY FACTOR NUBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23264">
        <property type="entry name" value="NUCLEOTIDE-BINDING PROTEIN NBP35 YEAST -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10609">
        <property type="entry name" value="ParA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01215">
        <property type="entry name" value="MRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 8" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0408">Iron</keyword>
    <keyword evidence="7 8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="13"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
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    <feature type="binding site" evidence="8">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="75"/>
            <end position="82"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>ligand shared with heterodimeric partner</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>ligand shared with heterodimeric partner</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR30234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="323" mass="34889" checksum="08D5AA3AC96FE6F9" modified="2009-07-28" version="1">MTEVSQILPPDYQLKEAEPEHCPGPESEKAGQGDACAGCANKEICESLPKGPDPDIPLITENLSQIKHKVLVLSGKGGVGKSTFTTMLCWALSSDEDLQIGAMDLDICGPSLPRMLGCVNETVHESNTGWTPVYVADNLATMSIQYMLPEDDSAIIWRGSKKNALIKKFLKDVDWDALDYLIVDTPPGTSDEHISINKFMKESGIDGALVVTTPQEVALLDVRKEIDFCRKAGIKILGLVENMSGFVCPSCKGESQIFKPTTGGGEKLCEELNIPFLGSVPLDPRVGKSCDEGQSFLDQHPESPASLAILDVVEKLRDAVGDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2L4</accession>
    <name>C5E2L4_LACTC</name>
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        <submittedName>
            <fullName evidence="3">KLTH0H05918p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0H05918g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="CAR30275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome H"/>
    </dbReference>
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        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14373">
        <property type="entry name" value="CUE_Cue3p_like"/>
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    </dbReference>
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        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
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    </dbReference>
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        <property type="entry name" value="ASCC2"/>
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        <property type="entry name" value="CUE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041808">
        <property type="entry name" value="Cue3_CUE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21494:SF0">
        <property type="entry name" value="ACTIVATING SIGNAL COINTEGRATOR 1 COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ACTIVATING SIGNAL COINTEGRATOR 1 COMPLEX SUBUNIT 2 ASC-1 COMPLEX SUBUNIT P100"/>
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    </dbReference>
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        <property type="entry name" value="CUE"/>
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    </dbReference>
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        <location>
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            <end position="353"/>
        </location>
    </feature>
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        <location>
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            <end position="599"/>
        </location>
    </feature>
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            <end position="538"/>
        </location>
    </feature>
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            <end position="550"/>
        </location>
    </feature>
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            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="574"/>
            <end position="599"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR30275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="599" mass="68898" checksum="4834A7AC81F050D0" modified="2009-07-28" version="1">MNLNRYQRVIEINGGDKISLPIVKFPPFKLRAAMVEKDPVIWLHLLETYNQYFTYLMSEGHLEQLEDSTYENLCMFIKSYLKELSNEEGKLLSLGMNTNVCEQLQVLRSWVLTVIKTCGLLHLQISCDVLWDFVRLYVKENPGTVRLLIDGSLKPAINTQKAQINRIYQLHQHLKQLIETSKFTRVDLKALENLLSAGDIHTKPFTNQFLTTKWAEILESWFSSDLKPLRDIAKKIGILSYLTASEDRIVELISESNVFDLESLHFHPLMGSLLINEQFQAHFSGLKAKLPFLRALLTKNNNKAPTISVEDVQTIIEVFPHLTVGQVERRLQAHDGDVERTINSFFESPTETDKAPLIDEVTRNKQEEMKNTNFESELGKADRFSKPDEVTTRDHVPDEVRNKTLTRALALLYQADEDEKDDTYDDAEVQRDALGNLDSDDRQSANGNNYDKIEGHLWELLKENKELFDRSNRNSKARKELKTSTSWSDEQIEGWARMLERSPKRAQILEERYMFRGNVRTGKRAFVQNKNESAAAQNKTERNNSVREAKGNSVAPKSTQQNGASDKKKQNARKEKHKSARANHNRKSGHDKKISRVSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2M7</accession>
    <name>C5E2M7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">KLTH0H06226p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0H06226g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ZC3H14 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556150.1">
        <property type="nucleotide sequence ID" value="XM_002556104.1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="STRING" id="559295.C5E2M7"/>
    <dbReference type="GeneID" id="8294465"/>
    <dbReference type="KEGG" id="lth:KLTH0H06226g"/>
    <dbReference type="eggNOG" id="KOG3702">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037973_1_0_1"/>
    <dbReference type="InParanoid" id="C5E2M7"/>
    <dbReference type="OMA" id="HAHPTKV"/>
    <dbReference type="OrthoDB" id="438553at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043488">
        <property type="term" value="P:regulation of mRNA stability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.40:FF:000003">
        <property type="entry name" value="Nuclear polyadenylated RNA-binding protein NAB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.40:FF:000005">
        <property type="entry name" value="Polyadenylated RNA binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.40">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.40">
        <property type="entry name" value="Nuclear abundant poly(A) RNA-bind protein 2, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040366">
        <property type="entry name" value="Nab2/ZC3H14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043094">
        <property type="entry name" value="Nab2/ZC3H14_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021083">
        <property type="entry name" value="Nab2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049017">
        <property type="entry name" value="Nab2_Znf4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041044">
        <property type="entry name" value="Nab2p_Zf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048410">
        <property type="entry name" value="Znf-CCCH_2-like_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14738">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14738:SF29">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11517">
        <property type="entry name" value="Nab2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21803">
        <property type="entry name" value="Nab2-zf4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18260">
        <property type="entry name" value="Nab2p_Zf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14608">
        <property type="entry name" value="zf-CCCH_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21457">
        <property type="entry name" value="zf-CCCH_2-like_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 8" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="10">
        <location>
            <begin position="234"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="8">
        <location>
            <begin position="234"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="97"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="170"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="457"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="108"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="459"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR30288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="510" mass="55990" checksum="4BCB169153C5CBD0" modified="2009-07-28" version="1">MSDPKEEIFNNLKVVIAENLKTLPNFNEDVNYVAEYIVLLMSNGGNLESVVQELTSLFDTVPPEAFHNVVQNAYKVLGYLQGGDSLEVAVSKLTGGSNAPAGQEQHNQQAMFSQEQQQQQQSQQQPQHQAHQQQPQQQAAVPSIFQTAPPATAAQPQSAFAGLVEFSPAPSGDISKSGGAQRGGGVGKASTRGGRNERPGRGGKNGVGKRNNYQNNNALAMALGMDDPNTNVVQKKQGRCKLFPRCPLGRQCPHAHPTQACREYPNCPNPPGTCNYLHPNEDVELMKEIEKTREEFREKRAAVAASRAKPINTGIVLCKFGNLCSNPQCPFGHPTPANEDAKVITFAWCPENLKCENPTCDRAHSSHSKIKEVAPIAARKPSRPPVEKSLEQCKFGQNCTNKRCKFRHARSHIMCREGANCTRIDCLFGHPINEDCKFGVECRNIYCLYRHPEGRQLPDKSQQAQSNSNNTWVNPNLGTGNSSSNERPFAVPENQVIEAAPAQEQDAMMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2W8</accession>
    <name>C5E2W8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Mitogen-activated protein kinase</fullName>
            <ecNumber evidence="10">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0H08316g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="10">Activated by threonine and tyrosine phosphorylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="10"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556241.1">
        <property type="nucleotide sequence ID" value="XM_002556195.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2W8"/>
    <dbReference type="FunCoup" id="C5E2W8">
        <property type="interactions" value="742"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2W8"/>
    <dbReference type="GeneID" id="8294559"/>
    <dbReference type="KEGG" id="lth:KLTH0H08316g"/>
    <dbReference type="eggNOG" id="KOG0660">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_181_1_1"/>
    <dbReference type="InParanoid" id="C5E2W8"/>
    <dbReference type="OMA" id="IFDIQRP"/>
    <dbReference type="OrthoDB" id="192887at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009272">
        <property type="term" value="P:fungal-type cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000040">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000073">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 9" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="13"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR30379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="355" mass="40348" checksum="EC604FCEE7D68AD3" modified="2009-07-28" version="1">MGKKIVFNISSEFQLQQMLGEGAYGVVCSARHLPTGQMVAIKKIEPFDRPLFCLRTLREIKLLRCFQHENIISIFDIQRPASFETFSEVYIIQELMDTDLHRVITTQTLSDDHVQYFVYQILRALKCLHGAEVIHRDLKPSNLLVNANCDLKLCDFGLARCAGGGTKPGDAGHMTEYVATRWYRAPEVMLTAAEYTFAMDVWSCGCILAELLMRRPLFPGKDYHHQLLLIFQVIGSPTAGDLGCVRSKRARQYLNTLPRYAGDGNLRSLVPDASAEALDLLEQMLVFDPRRRITASQALKHPYLQAYHDPQDEPATAKVDPALFHFDERKDKLGAGDLKRLLWDEIFGKRERTDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5E383</accession>
    <name>C5E383_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0H11154p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0H11154g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556356.1">
        <property type="nucleotide sequence ID" value="XM_002556310.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E383"/>
    <dbReference type="FunCoup" id="C5E383">
        <property type="interactions" value="174"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E383"/>
    <dbReference type="GeneID" id="8294944"/>
    <dbReference type="KEGG" id="lth:KLTH0H11154g"/>
    <dbReference type="eggNOG" id="KOG1450">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010730_2_0_1"/>
    <dbReference type="InParanoid" id="C5E383"/>
    <dbReference type="OMA" id="DSGWQAR"/>
    <dbReference type="OrthoDB" id="437889at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005933">
        <property type="term" value="C:cellular bud"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000063">
        <property type="entry name" value="Rho GTPase activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050729">
        <property type="entry name" value="Rho-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23176:SF128">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN RGD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23176">
        <property type="entry name" value="RHO/RAC/CDC GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00611">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00055">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-BAR" evidence="6">
        <location>
            <begin position="49"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="5">
        <location>
            <begin position="511"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="349"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="384"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="149"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="351"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="384"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="442"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR30494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="705" mass="78161" checksum="E06657BDC707C864" modified="2009-07-28" version="1">MSVSRSSSRTDQTSEANSALQPSKSPASGSNETPSAGSTGVQPDLLGKPEITRVLDSDVAINALLSRLKQSLLTCEEFTKFVRKKYLLEDVHAQELGKTYKNFFPDSSASSSLQKSIHTVLEFDGKLSQVKHSYVKALHTMSDELGALLLTMTKMRKGLKENSRRLEKEVADAIHMAEKAKSRYNSLCQDWEKLRMADPTKTKLTLRGSKTTREQEEDLLRKIDSADLDFKQKVDHSTSLRNTFLSRERPKIVSELKDLILELDTAMAIQLQKYTIWTETLILNSGVTVTPFDATKSMKSIAASTSNELDLYNFLNKYNQGSRSNNFVNKNLIPVEYKQYPSMAKPVMAKNGQTRSGNHTSSSQNTFSVNTNMNSLPKRIVSTQHESPFDSTPTPRTGFSPVQNGSPAGSPNSHPGNASRGNLAQTTPSHQKAVSSMLDVADSQTPRSSQSGTRPDGPDSDFATLDPGNGTRSTSLTTSMITSISERPVSHIQSAATLPPGINRNFKTFGVALESLLEYEQDMVPAIVRQCIYVVDKFGLDLEGIYRKPANVLDVSKLREEIDKDPSNISMIVPPKNHTDSDIYLAGSLLKAFFANLPESLLPPSMTSEIKTCLTIADPVTRKNYMHGLIYKLPDGQYWTLRSLVFHLKRIIEHEPTNRMNLKSVCIIWGPTLIAPNDTDINDVNYQIQAMEVLIDVADQAFEPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3K1</accession>
    <name>C5E3K1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="2">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0H14234g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="2"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556474.1">
        <property type="nucleotide sequence ID" value="XM_002556428.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3K1"/>
    <dbReference type="FunCoup" id="C5E3K1">
        <property type="interactions" value="82"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3K1"/>
    <dbReference type="GeneID" id="8294836"/>
    <dbReference type="KEGG" id="lth:KLTH0H14234g"/>
    <dbReference type="eggNOG" id="KOG0546">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012062_37_0_1"/>
    <dbReference type="InParanoid" id="C5E3K1"/>
    <dbReference type="OMA" id="QFFITTY"/>
    <dbReference type="OrthoDB" id="407558at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016018">
        <property type="term" value="F:cyclosporin A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF561">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0697">Rotamase</keyword>
    <keyword evidence="5" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="6">
        <location>
            <begin position="7"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="5">
        <location>
            <begin position="332"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR30612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="395" mass="44764" checksum="5CF914344CA89E29" modified="2009-07-28" version="1">MSRSNVYMDIKIGDEKVGRIVIELFDDKAPKSSKNFYHLCKGDAQIGSRQLWYKNNFFHRVIQSFMVQAGDIIYGSKGPEKSDDIGKGGCSIFGKKDELDLDSTLETVGGVRCCGIFEDENLGDFTEPFMVAMANMGEADTNSSQFFITTCSSPHLNNKHSVIGRVVYGKSVVRSIEHTAVDSDGFPTTPVLIEDCGEWNETLGIPLYNASNNTIGGDVFEEYPDDDLNFDKDSAEKAYEASCIIKDSGSLLFKSKDYQNAYFKYRKALKYVNEFIPDIDMDKVFYEKFTELKAKLYLNLILVMFNQKKYEDAIKYSVYLLEIPEASGLDRAKAYYRSGNCHLMKKRFDKALADFNACKEHNPNDEAVDKKIGEAQNKLEESKERTRKSIAKFFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3L6</accession>
    <name>C5E3L6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">pyridoxal kinase</fullName>
            <ecNumber evidence="2">2.7.1.35</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0H14586g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the pyridoxine kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.35" evidence="2"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556489.1">
        <property type="nucleotide sequence ID" value="XM_002556443.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3L6"/>
    <dbReference type="FunCoup" id="C5E3L6">
        <property type="interactions" value="318"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3L6"/>
    <dbReference type="GeneID" id="8294852"/>
    <dbReference type="KEGG" id="lth:KLTH0H14586g"/>
    <dbReference type="eggNOG" id="KOG2599">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046496_1_0_1"/>
    <dbReference type="InParanoid" id="C5E3L6"/>
    <dbReference type="OMA" id="HTQYGQW"/>
    <dbReference type="OrthoDB" id="2104723at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008478">
        <property type="term" value="F:pyridoxal kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009443">
        <property type="term" value="P:pyridoxal 5'-phosphate salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01173">
        <property type="entry name" value="pyridoxal_pyridoxamine_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013749">
        <property type="entry name" value="PM/HMP-P_kinase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004625">
        <property type="entry name" value="PyrdxlKinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00687">
        <property type="entry name" value="pyridox_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10534">
        <property type="entry name" value="PYRIDOXAL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10534:SF2">
        <property type="entry name" value="PYRIDOXAL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08543">
        <property type="entry name" value="Phos_pyr_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyridoxamine kinase/Phosphomethylpyrimidine kinase" evidence="7">
        <location>
            <begin position="37"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR30627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="304" mass="34057" checksum="F7833B9CB6136AB2" modified="2009-07-28" version="1">MPRLLATQSHVVHGYVGNKAATFPLQCMGWDVDCLNSVQFSNHTGYGRDRVFGTCTSAQQLESVFEGLAAFGDYDAVLSGYLPNRESVACMARNYKKMKERQPELIWLLDPVMGDDGQLYVNEDVIPEYEALVTSSLVDVITPNQFELEILYGKHISDLASLRRALEKLHETVPVIVVTSCSSELFGDPSHLYCVASTRGHPATVYRVPVIESYFTGVGDLFSALLLDRLYNTRQDSGSSHLDINQVLDVVQKVLATTLQHSPANIKAAMGSPIEMKLMELRIVESRDHYVQFECPRNFIYRQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3R2</accession>
    <name>C5E3R2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0H15664p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0H15664g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556535.1">
        <property type="nucleotide sequence ID" value="XM_002556489.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3R2"/>
    <dbReference type="FunCoup" id="C5E3R2">
        <property type="interactions" value="111"/>
    </dbReference>
    <dbReference type="GeneID" id="8294901"/>
    <dbReference type="KEGG" id="lth:KLTH0H15664g"/>
    <dbReference type="eggNOG" id="ENOG502S148">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069850_0_0_1"/>
    <dbReference type="InParanoid" id="C5E3R2"/>
    <dbReference type="OMA" id="NDCNTTE"/>
    <dbReference type="OrthoDB" id="3981131at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="212"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="256"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="25"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="224"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="271"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR30673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="334" mass="37227" checksum="35EB590542B8438F" modified="2009-07-28" version="1">MAKIGELPNDALVDTSVRRQDSELSRSSCLSPSPDDSLNEESQPIIEKAKSLIDTLKTERILDSSVHPSSPNANTSSGRDLGVRSHSESPRASDALSKDTESKREIVQSLESLVQLASKAREQAKQLRFKNFMLTAGLRDTNSRFEVETSLTKQQFERIRCQLVMECQELQEKVHLQDLKLSKYKETIIEKNKEINKLGRFLNDSGLGKSLHVRNTPQSVTKKAHLDRTSKAQRKDSGMLATLGLLASQVLGDKSEHSVNVDNTESDISHDSFSNQTQGNKAQAAKPFLSTPILPKITRTSFTSPHDDTSSEHQFQIPKLRSFSTLEDSLKDPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3T5</accession>
    <name>C5E3T5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0H16170p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0H16170g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556558.1">
        <property type="nucleotide sequence ID" value="XM_002556512.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3T5"/>
    <dbReference type="STRING" id="559295.C5E3T5"/>
    <dbReference type="GeneID" id="8294924"/>
    <dbReference type="KEGG" id="lth:KLTH0H16170g"/>
    <dbReference type="HOGENOM" id="CLU_441497_0_0_1"/>
    <dbReference type="InParanoid" id="C5E3T5"/>
    <dbReference type="OMA" id="FHETEGV"/>
    <dbReference type="OrthoDB" id="5069333at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050675">
        <property type="entry name" value="OAF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069">
        <property type="entry name" value="OLEATE-ACTIVATED TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069:SF12">
        <property type="entry name" value="TRANSCRIPTION FACTOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="7">
        <location>
            <begin position="14"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR30696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="619" mass="71377" checksum="307FEDB8EEF4EC3B" modified="2009-07-28" version="1">MHNKQTKRNRRLHVCDACRIRKLKCDKAKPNCSRCAKHGLECIYNPYRQKEEPETVVALKKELLELRAQLVANSPASCTSEVSKPKISWDIFVTPVKVGGVWRSYYSPLSDVALFARDPFLRGLNADLFEQETAFHETQFNLKTQDLTETDPRTNIKRLLPDNEVFMKSKTIFYTQLYQHYTFFQKDLFESLFEEVILKGNLDNEHAPFVLMSSLLISCLTNALSVPAEVIFHYADQLKIITLPSEHNLSCLLYLKIYSGDMKLCDSLYDTLSSSIRNMTMNLGLFASRLPNENANIKRNLWMGAVALLEPTSFDQFFVKKFEFPNSEHTHTLWYEYNFHLKLSQVWRCVLTSSSQLEFTIAQLKSYYKKKVPNNADKFLRASCELSLIAIELNILWMFCMQNELQNRPFDEPLLVLLEEKFTGIHSLLTKDFCNLNKDTIRIKSLGFTLKTFIVYAIGMSLRQQKRDSHPIHKGLHEGFNFREMVSEVFLLWKNDAPWYRTLIRLWDFHETEGVLENFQQNLTDESQSLLSVTKASESTEEFRFGQLESPILETKTSAVSLSSSSSCSGSSSAFYEASDLINFDDAIDPMLAADDSCLEIDFFSCPASQEFKLDVNTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3T7</accession>
    <name>C5E3T7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0H16214p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0H16214g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556560.1">
        <property type="nucleotide sequence ID" value="XM_002556514.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3T7"/>
    <dbReference type="STRING" id="559295.C5E3T7"/>
    <dbReference type="GeneID" id="8294926"/>
    <dbReference type="KEGG" id="lth:KLTH0H16214g"/>
    <dbReference type="eggNOG" id="KOG0254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_29_0_1"/>
    <dbReference type="InParanoid" id="C5E3T7"/>
    <dbReference type="OMA" id="GMFGPVM"/>
    <dbReference type="OrthoDB" id="440755at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY MULTIDRUG TRANSPORTER MFSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718:SF9">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY MULTIDRUG TRANSPORTER MFSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="30"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="98"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="157"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="188"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="232"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="265"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="321"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="354"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="386"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="413"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="498"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="33"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="526"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="533"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR30698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="551" mass="60107" checksum="45CA6A106F100C96" modified="2009-07-28" version="1">MANMHTTAAPKLSEGIYNLRRRCQNSKYQLIINMALIFLGCTFDLLNVAGVISVIEDLGETYGLEYTTASWALTSYAITFAGLIAFMGRLGDIIGNSVLFTISGFFFSLFCLLCAVVTNFPAFAVLRALQGIAAAGLVPCGYALIPTLADPEDVQLFFAVVSCGFSSTIGIGLIIGGAFALTNVGYKGIFYLSFAGMLLVSIAAFFFTYRIEEHKFAEQDYEGSIHRPGKVASLDFVGSLIFISGSVLIVVGLTEGGESWKRPVAYVPFTIGMIFFCGFFAWNCSYQNLVIFLKKLGCDTSRYLEKVQVLIPRDVLFMTNFIPITLGFLCNGGCLYSNIYIIDQYSQYVDKNSPLLAGVKLVPLIITMVIGNTICASKSVKLRPRIGVALGFLSMVCGSIILIQLHLVEENLYWKIMFCSQILVGFGAAIFYPYALKLVVGDAPSGAKGIASGVTQTFAQLGIEMTFSVMVSVLGNITQVKSRPDSFERFRDGFQKCTYFTLASGAFGLVLTTFFVRGPRFSNGSPKDATALENGSYSQTESNCEKKEQQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-04-02" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>C5DB87</accession>
    <name>C5DB87_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0A00550p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0A00550g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551486.1">
        <property type="nucleotide sequence ID" value="XM_002551440.1"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DB87"/>
    <dbReference type="GeneID" id="8290277"/>
    <dbReference type="KEGG" id="lth:KLTH0A00550g"/>
    <dbReference type="eggNOG" id="ENOG502S1H2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_262460_0_0_1"/>
    <dbReference type="InParanoid" id="C5DB87"/>
    <dbReference type="OMA" id="GGIGQSH"/>
    <dbReference type="OrthoDB" id="4070545at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018789">
        <property type="entry name" value="Flo11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10182">
        <property type="entry name" value="Flo11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01213">
        <property type="entry name" value="Flo11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51824">
        <property type="entry name" value="FLO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950398" evidence="2">
        <location>
            <begin position="20"/>
            <end position="1288"/>
        </location>
    </feature>
    <feature type="domain" description="Flo11" evidence="3">
        <location>
            <begin position="36"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="227"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="433"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1080"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1199"/>
            <end position="1243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="227"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="385"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="433"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="457"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="467"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="723"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="740"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="763"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="841"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="864"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="888"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="911"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="962"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1199"/>
            <end position="1242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR21044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1288" mass="127939" checksum="FED911546FE82BDD" modified="2009-07-28" version="1">MRCIWALSALTALTSTAVGSPLQKRCSNPRQNTGPKESQATCTEITNGCPNLDFTWKPKNTGQMLYDISMKKVQWVKDNIYEITVHVQGEKNIAISCIDGLKVIGINGPDGGTFWLYDRNNNIAKVDSPTDYDATFRVYGQEDTSNCRVWMPNFQIQYDYNNQCDVYGNSKSFGLITGCNNYDNQGHSKTDAPGFYWSYQCSGKSSCGASSASSVFSTTQLTSATSKTCTKNKPTTTSCTKNKPTTTSCTKSKTQTTSESSSTETTETYTKPSCTKSKTQTTPETPSTETTETHTKPSCTKSKFSKTSCTKSKTQTTPETPSTETTETHTKPSCTKSKFSKTSCTKSKTQTTPETPSTETTETHTKPSCTKSKFSKTSCTKKSKSTTPLTPSTAPGTTVPSTTYYPSSSAPVSESSSTCQTVVTSTVSGSTTVYTTTCSTPVSETTTPETPVGPSTAPGTTIPGTRSCSTSPGTTVPGTTYYPSSSSSGFGNSSVPVTSAPSSSAPVSEFSSTPVSSAPSSSAPVSESSSTCQTVVTSTVSGSTTVYTTTCSTPVSETSSTPETTAPSSSAPVSEFSSTPVSSAPSSSAPVSEFSSTPVSSAPSSSAPVSEFSSTPVSSAPSSSAPVSESSSTCQTVVTSTVSGSTTVYTTTCSTPVSETSSTPVSSAPSSSAPVSSVPHSTPYYHNTTTAAGTGSTSYPVSLSSTPVTSGSGTPATSGSVPATSGSVPATSGTPATSGSVPATSETTPATSGTPATSGSVPATSETTPATSGSVPATSGTPATSGSVPATSETTPATSGSVPATSGTPATSGSVPATSETTPATSGSIPATSGTPATSGSVPATSETTPATSGTPATSGSVPATSETTPATSGSVPATSGTPATSGSVPATSETTPATSGTPATSGSVPATSETTPATSGSVPATSGTPATSGSVPATSETTPATSGSVPATSGTPATSGSVPATSETTPATSGTPATSGSVPATSGSIPTTSTCQTIVKSAVSGTTTIFTTTCPESTLPTTTGPAQTESVVTVTESGTVTSYTTTCPITEVEPTTTGPDNAKTVLVSSVSGTVTSYTTTLPASESQPATTAPASSESVVVSSASGTVTSYTTTLPASESQPATTAPASSESVVVSSASGTPVTESIVTDTVSGTTTIYTTTCPVSSAVETTSPGPANSEIVTTSTISGTETVYTTTAPLSSAQKTEAASPSGSQTKSVESSPAYSGSYTTPGASGSYVTQTPSSSLEVQSSSSVAAVSTTAGVSTFEGAANKLRAGLLLAIPLALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBF1</accession>
    <name>C5DBF1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Type 1 phosphatases regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0A02090g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Regulator of type 1 phosphatases which maintains protein phosphatase activity under strict control.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the YPI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551550.1">
        <property type="nucleotide sequence ID" value="XM_002551504.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBF1"/>
    <dbReference type="FunCoup" id="C5DBF1">
        <property type="interactions" value="176"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBF1"/>
    <dbReference type="GeneID" id="8290346"/>
    <dbReference type="KEGG" id="lth:KLTH0A02090g"/>
    <dbReference type="eggNOG" id="KOG4102">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098333_3_0_1"/>
    <dbReference type="InParanoid" id="C5DBF1"/>
    <dbReference type="OMA" id="RRHIQWA"/>
    <dbReference type="OrthoDB" id="307488at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008157">
        <property type="term" value="F:protein phosphatase 1 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004865">
        <property type="term" value="F:protein serine/threonine phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011107">
        <property type="entry name" value="PPI_Ypi1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20835:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PPP1R11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20835">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PPP1R11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07491">
        <property type="entry name" value="PPI_Ypi1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="89"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="53"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="102"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="116"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="128"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="157"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU367162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR21108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="166" mass="18674" checksum="94B92E9FBCB7F631" modified="2009-07-28" version="1">MRAGKNAGSSRTRALDSTTTSTVQESAPVLQLRGSPNDQREGATHTPAATGSQEKREVRWKEDVVDNEHMNKKKSKVCCIFHPQQNFEDMDDECDHCGHSDSSSSSSSSSSSSESESEKDLDRESRHNARKERRKRKLAQKRSTSPNAYEIQPDYSQNKDKQTNNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBJ9</accession>
    <name>C5DBJ9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">GPI inositol-deacylase</fullName>
            <ecNumber evidence="10">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0A03234g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Involved in inositol deacylation of GPI-anchored proteins which plays important roles in the quality control and ER-associated degradation of GPI-anchored proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the GPI inositol-deacylase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="10"/>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551598.1">
        <property type="nucleotide sequence ID" value="XM_002551552.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBJ9"/>
    <dbReference type="FunCoup" id="C5DBJ9">
        <property type="interactions" value="64"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBJ9"/>
    <dbReference type="GeneID" id="8290398"/>
    <dbReference type="KEGG" id="lth:KLTH0A03234g"/>
    <dbReference type="eggNOG" id="KOG3724">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006103_0_0_1"/>
    <dbReference type="InParanoid" id="C5DBJ9"/>
    <dbReference type="OMA" id="WVRNLAV"/>
    <dbReference type="OrthoDB" id="348976at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050185">
        <property type="term" value="F:phosphatidylinositol deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006505">
        <property type="term" value="P:GPI anchor metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012908">
        <property type="entry name" value="PGAP1-ab_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039529">
        <property type="entry name" value="PGAP1/BST1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056824">
        <property type="entry name" value="PGAP1_TMD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15495:SF7">
        <property type="entry name" value="GPI INOSITOL-DEACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15495">
        <property type="entry name" value="NEGATIVE REGULATOR OF VESICLE FORMATION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07819">
        <property type="entry name" value="PGAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25141">
        <property type="entry name" value="PGAP1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25140">
        <property type="entry name" value="PGAP1_TMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="67"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="714"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="755"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="810"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="875"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="928"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="990"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1015"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="domain" description="GPI inositol-deacylase PGAP1-like alpha/beta" evidence="12">
        <location>
            <begin position="146"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="GPI inositol-deacylase transmembrane" evidence="13">
        <location>
            <begin position="716"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="32"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF07819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF25140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR21156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1035" mass="117448" checksum="351309A069E668FD" modified="2009-07-28" version="1">MGLRRSVNALAHKGVYFAGRYLKMGEHKISNSAGSEVGGAQTKRKRSGLKSVTGDALRNQKSSGDKVPLRIITLGFLLVLLVVAASFFKKFTGADSPKCRSIYMYPSYARIDGFDERFTKLAKKYHLYLYREQGKDREPLENNLIQLDGVPVLFIPGNAGSFKQARSIAAASADMYFDEPELIDNHWTKNLDFFTADFNEDFTAFHGRSLLDQAEYLNDAIKYILSLYSANEDRTEPQPQSVLVLGHSMGGVVARVMPTLKNFVPESINSYITLSAPHTASPVTFDGDMLKIYESTSKFWKSQFSDNSSFFSQNVSLISITGGVLDTTLPADLTLVEDIIPYSNGFTTYTTTIPQVWTPIDHLAIVWCDQLRKVLAKLLLETVDVHSSYKSRPLKERMRFYRKSLISGLEDYAVQDLKIDDRSESSYKPFELPDDFEEMVTGEKLILTRETAEAHRLLNLFKIPTNKKDYQFSLLTSMAPPEVFFCQNNIVRARIDDKLEDTKFECVSAKDDFTRIPRSTKDSLFPSDSSVGEPMSPFYLLKINGAVLSRFEFVAFSAPEGFVHDDDFLIAEMKATNSTRSLGDNPLTVLLKSLIKRKVRIDTSFLISTISFPNLWSSVISYRLKTTTIGENLIFEPLIRQFIELPFETKWHVNLKQGPQDVNFHNVAPFIPSNSSNDRSLKLDFISPPGCEMTMSLEINWALTMKMLLIRFRLTIAAFTVGLVSLVAFLQYYQYNQTGQFLAFDAALKLLLSKYWVLILALASLLTPLYSFFCLEKLSNITKFADRTRPTMSASHSWASNTFFLGIDELFLWWLGPVFFLMTVSSVYLLFRLITMVEAAARVAFSKVLPFRRTVEVSSIANEASSNRQFNLRQFAGALILVLGVVFYIPYQFAFIILTVVQSLFCLKIAVSAEEGIKRNVVNYNNSVLLLMLLLLPINAPIVMVFMRNFAIRWETPFRSHHNCLAILPIILLIESNARSRIPLRSLTNNLVSLAILGWLLHMSLYSFIFGTRNLYWLHYLFNSLCAMLFLKTFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBM2</accession>
    <name>C5DBM2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0A03740p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0A03740g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551621.1">
        <property type="nucleotide sequence ID" value="XM_002551575.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBM2"/>
    <dbReference type="FunCoup" id="C5DBM2">
        <property type="interactions" value="203"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBM2"/>
    <dbReference type="GeneID" id="8290421"/>
    <dbReference type="KEGG" id="lth:KLTH0A03740g"/>
    <dbReference type="eggNOG" id="KOG0691">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025145_3_1_1"/>
    <dbReference type="InParanoid" id="C5DBM2"/>
    <dbReference type="OMA" id="DMKIESF"/>
    <dbReference type="OrthoDB" id="552049at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016558">
        <property type="term" value="P:protein import into peroxisome matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000028">
        <property type="entry name" value="DnaJ domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026894">
        <property type="entry name" value="DnaJ_X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052814">
        <property type="entry name" value="Peroxisomal_DnaJ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45006">
        <property type="entry name" value="DNAJ-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45006:SF2">
        <property type="entry name" value="PROTEIN CAJ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14308">
        <property type="entry name" value="DnaJ-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="3">
        <location>
            <begin position="6"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="122"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="133"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR21179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="395" mass="44477" checksum="9C16A9E08F2A97C7" modified="2009-07-28" version="1">MVKDTEYYDLLGIQPDASATEIKKAYRKKAMLTHPDKHPDDPEAQAKFQAIGQAYQVLSDPELRSRYDEFGKEDAVPQQGFEDAAEFFSTIFGGDAFQDWIGDFAFLKNLTKGAEIMGEDGEEAGTAAENSEDPSKDVVQHDGKTAKPKSSDNKLTKEQRAKLVEMENERRAEKKKQVEDLVRKLETRIEQYVAAVQNKHLDEFDAKLNQEIEDLKLESFGLELLQLIAKVYKTKANNFLASQKTYGFSKLFTGVRDKTKTAKSAWGILSSAMDAQSAMKELEKLDVETMDEYERAEVEKLITGKVLGTAWVMSKFEAQGKLKDVCDKILGDKNVPSKQRVVKAKALLYMANKFASAQRSPDEAEDARVFEELIFDAQGKKKKADHKRKDSTVKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBP9</accession>
    <name>C5DBP9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0A04400p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0A04400g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the SAP domain-containing ribonucleoprotein family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551648.1">
        <property type="nucleotide sequence ID" value="XM_002551602.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBP9"/>
    <dbReference type="FunCoup" id="C5DBP9">
        <property type="interactions" value="49"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBP9"/>
    <dbReference type="GeneID" id="8290451"/>
    <dbReference type="KEGG" id="lth:KLTH0A04400g"/>
    <dbReference type="eggNOG" id="ENOG502SARN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088651_1_0_1"/>
    <dbReference type="InParanoid" id="C5DBP9"/>
    <dbReference type="OMA" id="WSEKDNA"/>
    <dbReference type="OrthoDB" id="445357at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.30">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003034">
        <property type="entry name" value="SAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036361">
        <property type="entry name" value="SAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052240">
        <property type="entry name" value="SAP_domain_ribonucleoprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040746">
        <property type="entry name" value="THO1_MOS11_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46551">
        <property type="entry name" value="SAP DOMAIN-CONTAINING RIBONUCLEOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46551:SF1">
        <property type="entry name" value="SAP DOMAIN-CONTAINING RIBONUCLEOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02037">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18592">
        <property type="entry name" value="Tho1_MOS11_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00513">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68906">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50800">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAP" evidence="4">
        <location>
            <begin position="7"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="148"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="11"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="42"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="66"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="87"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="175"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00046328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR21206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="197" mass="21146" checksum="35F500359AB39433" modified="2009-07-28" version="1">MAPMAAYAKRTVPELRKELESRQLSHDGNKPDLIARLEAHDAAQAAVEPPTAATAQEEPAPEPEAQPETQPEAQPAPESAPEPAPESAPAQPAQEHQASPAPRKPSPEEAKALALADLELKLRRAKKFADDQSTIDSLQRQIARVHKFGVDPTTQLARDLGLGSGPASAAPKSANRPRSRRRKANPQSKSTKHARSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBU9</accession>
    <name>C5DBU9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">KLTH0A05522p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0A05522g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the adrenodoxin/putidaredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551698.1">
        <property type="nucleotide sequence ID" value="XM_002551652.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBU9"/>
    <dbReference type="FunCoup" id="C5DBU9">
        <property type="interactions" value="478"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBU9"/>
    <dbReference type="GeneID" id="8290502"/>
    <dbReference type="KEGG" id="lth:KLTH0A05522g"/>
    <dbReference type="eggNOG" id="KOG3309">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082632_0_1_1"/>
    <dbReference type="InParanoid" id="C5DBU9"/>
    <dbReference type="OMA" id="QSPWQGY"/>
    <dbReference type="OrthoDB" id="268593at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140647">
        <property type="term" value="P:P450-containing electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000013">
        <property type="entry name" value="Adrenodoxin, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001055">
        <property type="entry name" value="Adrenodoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018298">
        <property type="entry name" value="Adrenodoxin_Fe-S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23426:SF65">
        <property type="entry name" value="FERREDOXIN-2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23426">
        <property type="entry name" value="FERREDOXIN/ADRENODOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00111">
        <property type="entry name" value="Fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00355">
        <property type="entry name" value="ADRENODOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00814">
        <property type="entry name" value="ADX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="11">
        <location>
            <begin position="51"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR21256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="162" mass="17650" checksum="0BFA0AF03EE5F25D" modified="2009-07-28" version="1">MFRIAGLSKSLLRGPLAVGRANIMVPKYAGFAQSALRFHGHVHKPKPGEELHVTFILKDGSQRQYEVSAGDTLLDIAQANNLEMEGACGGSCACSTCHVIVDPDYYDALEEPDDDENDMLDLAYGLTETSRLGCQIKMSKDIDGIRVALPAMTRNVSNTDFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBZ6</accession>
    <name>C5DBZ6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Putative lipase ATG15</fullName>
            <ecNumber evidence="6">3.1.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Autophagy-related protein 15</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Putative lipase atg15</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="ordered locus">KLTH0A06622g</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Lipase which is essential for lysis of subvacuolar cytoplasm to vacuole targeted bodies and intravacuolar autophagic bodies. Involved in the lysis of intravacuolar multivesicular body (MVB) vesicles. The intravacuolar membrane disintegration by ATG15 is critical to life span extension.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12044"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17855"/>
            <dbReference type="ChEBI" id="CHEBI:18035"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="EC" id="3.1.1.3"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Binds to both phosphatidylinositol (PI) and phosphatidylinositol 3,5-bisphosphate (PIP2).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endosome</location>
            <location evidence="3">Multivesicular body membrane</location>
            <topology evidence="3">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Prevacuolar compartment membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.3" evidence="6"/>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551745.1">
        <property type="nucleotide sequence ID" value="XM_002551699.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBZ6"/>
    <dbReference type="FunCoup" id="C5DBZ6">
        <property type="interactions" value="83"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBZ6"/>
    <dbReference type="GeneID" id="8290552"/>
    <dbReference type="KEGG" id="lth:KLTH0A06622g"/>
    <dbReference type="eggNOG" id="KOG4540">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028295_0_2_1"/>
    <dbReference type="InParanoid" id="C5DBZ6"/>
    <dbReference type="OMA" id="CHDCYNW"/>
    <dbReference type="OrthoDB" id="58570at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032585">
        <property type="term" value="C:multivesicular body membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005775">
        <property type="term" value="C:vacuolar lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004620">
        <property type="term" value="F:phospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034496">
        <property type="term" value="P:multivesicular body membrane disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046461">
        <property type="term" value="P:neutral lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006660">
        <property type="term" value="P:phosphatidylserine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00519">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050805">
        <property type="entry name" value="ATG15_Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002921">
        <property type="entry name" value="Fungal_lipase-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47175">
        <property type="entry name" value="LIPASE ATG15-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47175:SF2">
        <property type="entry name" value="LIPASE ATG15-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01764">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0072">Autophagy</keyword>
    <keyword evidence="10" id="KW-0967">Endosome</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="16" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="17 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="9 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 22" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="Fungal lipase-type" evidence="23">
        <location>
            <begin position="296"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="476"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="479"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00018542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00019241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00024663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00029828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF01764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="CAR21303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="515" mass="58162" checksum="3E8F2FADEFDD6B45" modified="2009-07-28" version="1">MDDTKSLKRRTRFRIFKSHLGLASFVLATICAVLLWYKKHEYTSVSIRDRGSSQSSFRLTRTYRHGISQDPYMQVLDIDEQVLQAGQERFMEHVSMLSDEEAQDDDMWTSKPEYSTSNPLDFEFRLASEPLRMKRMLHRDPQFVESYLDFSHENPHMAAKVNLEWIDKTVLVPNVSDKQTVISLALMSSDAYVRLPHTGDWRNLTEPWNHTHNSGHGWENDGLRGHLFVNSEQDIVVMAVKGTSARGLAGSGEDETTVNDQINDNLLFSCCCARISYLWTTVCDCYMKSYTCDETCLEQELSRKDRYYKAVLNFYKDVVAEYPNSTIWVTGHSLGGALASLLGRTYGLPVVAFEAPGELLATRRLHLPMPPGYPDYLEGIWHFGNTADPIFMGTCNGASSSCSIAGYAMETSCHTGKVCVYDVVKDKGWHVSMLNHRIHKVIDGVIDSYEGPARCIPPGPCHDCYNWKYVQKGHSSSVSQTRSPKTTSTSTVPPEPTITSSCVGRNWIGICTEYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5DC01</accession>
    <name>C5DC01_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein mL49</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0A06732g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mL49 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551750.1">
        <property type="nucleotide sequence ID" value="XM_002551704.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DC01"/>
    <dbReference type="FunCoup" id="C5DC01">
        <property type="interactions" value="171"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DC01"/>
    <dbReference type="GeneID" id="8290557"/>
    <dbReference type="KEGG" id="lth:KLTH0A06732g"/>
    <dbReference type="eggNOG" id="KOG4034">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_132729_1_0_1"/>
    <dbReference type="InParanoid" id="C5DC01"/>
    <dbReference type="OMA" id="PHISKKA"/>
    <dbReference type="OrthoDB" id="19439at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.780.10">
        <property type="entry name" value="SUI1-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007740">
        <property type="entry name" value="Ribosomal_mL49"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13477:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13477">
        <property type="entry name" value="MITOCHONDRIAL 39S RIBOSOMAL PROTEIN L49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05046">
        <property type="entry name" value="Img2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR21308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="172" mass="19239" checksum="A6ADAF7BBF8E675F" modified="2009-07-28" version="1">MLVRFGLRTARHQGGLVCSGLQTIRPAMRLASSSLDINSGKLDDGRELSALTEQEINDLSTAFEEQTFSVFPRLQDVKPEELVGFAKFGVQSYFIERSATGNLPVYTDFKRGGKVVTEIRKIHGDPVQLRNDLQARLSHLPKDSFKVMMQAKKIIIEGDVVRQVKEALSSKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>C5DC35</accession>
    <name>C5DC35_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA mismatch repair protein MSH3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4 12">DNA mismatch repair protein MSH3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">MutS protein homolog 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0A07480g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA mismatch repair MutS family. MSH3 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551784.1">
        <property type="nucleotide sequence ID" value="XM_002551738.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DC35"/>
    <dbReference type="FunCoup" id="C5DC35">
        <property type="interactions" value="872"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DC35"/>
    <dbReference type="GeneID" id="8290591"/>
    <dbReference type="KEGG" id="lth:KLTH0A07480g"/>
    <dbReference type="eggNOG" id="KOG0218">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002472_0_2_1"/>
    <dbReference type="InParanoid" id="C5DC35"/>
    <dbReference type="OMA" id="INMHAAR"/>
    <dbReference type="OrthoDB" id="121051at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
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            <begin position="882"/>
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            <begin position="1"/>
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    <sequence length="1035" mass="117725" checksum="8A3420A97C2C4BB8" modified="2009-07-28" version="1">MSYQPAISKFFKSNSKNSAKRSASGNGNTGSPSPQPVTGSRSQSSGFWKDTARKSGDGTAEKHEANAADPARAVSKVAKAALSEYSFTRAKSTEDAPSRRGIQESDDFSEKVQSALRKRASGTLDREADQDSSTDAAAIEPASKKSRKSDKLTPLDQQVKDLKLQHMDKILAVRVGYKYKFFAMDAVIVSKILQIMLISGKLTLDDSHPCDRSYKQLAYCSIPDNRLQVHLQRLLRHNLKVGVVEQAETQAIKRAHGTSSGVFRRKVDKVFTKATFSINETFNRAEANSTGELSTIWALKAAEDEHFFHFWLLSVQLSSGEVIHDKFSDRKKMSAIELDKRISYLSPIEVVSRGPLPEVIVSFIKKRQSEVQFYDLSTSEPEAPQGSHLGGIKLPEELLELRATLRHYLENYDTHKILDILHNYKPFDTKTNMILSPNTLESLEIFENQTDLTTRGSLMWILDHTRTPYGYRLLREWISRPLTDKNEINKRLDAIECARKEITSIFMEALSNMLKDTPDFLKNLNRISYGQTSRQEVYVFLKHLDKIAKHFISHHYYLQEQVFDTGGRVHQRSTLLTSVLQQLDDALRKINVTHLLNMINVEGALDKNKEKSCNEFFDLNNYDNAEVIISKLRDIDSVKEELQRELERIRRVLRRPRFFFKDEIEYLIEVRNTQLNGLPTDWVKVASTKTLSRFRTPETTKLVNQLEYHKNLLTYVCDEEFKKFLVRISADYAQLRDIINKLATFDCILSISAASFNRNYVRPKFSENSRQINIKNGRNPIIESLDVNYVANDVHMKEDANKLMIITGPNMGGKSSFIRQVALIGILAQIGSFVPADSAEIPIFNNVFTRIGAHDNLIKGDSTFKVEMLEMLDILKNCNENSLLLLDEVGRGTGTTDGVSISYAIIKHFTSMETKCPFILFITHYPILGSITSPLLDNYHMSFIEERRPGERWPTAVFLYKLTRGLASDSYGLNVARLANIPTDIINKAFEISEQAKFEMEGDKHLRFISAVKAALLDKGSSYKEKLLRLLEIEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCE3</accession>
    <name>C5DCE3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0B02354p</fullName>
        </submittedName>
    </protein>
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        <name evidence="5" type="ordered locus">KLTH0B02354g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
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    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_002551846.1"/>
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        <property type="component" value="Chromosome B"/>
    </dbReference>
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        <property type="entry name" value="SH3_Bbc1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="AIM3_BBC1_C"/>
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        <property type="entry name" value="Mti1_SH3"/>
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        <property type="entry name" value="SH3-like_dom_sf"/>
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        <property type="entry name" value="SH3_domain"/>
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        <property type="entry name" value="RHO-TYPE GUANINE NUCLEOTIDE EXCHANGE FACTOR, ISOFORM F"/>
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        <property type="entry name" value="RHO-TYPE GUANINE NUCLEOTIDE EXCHANGE FACTOR, ISOFORM F"/>
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    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
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            <begin position="5"/>
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        </location>
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            <begin position="323"/>
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        <location>
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            <begin position="394"/>
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        <location>
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            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
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        </source>
    </evidence>
    <sequence length="1214" mass="131478" checksum="266743DF84EBECBC" modified="2009-07-28" version="1">MEEPEPPFTVIAQFPYTSEHDDDLNFEKGSLITVQSIEDDEWYFGEYQDESGTYKEGIFPRSFVKLAGDEEPSKPVEDPLDRVPSPTQNPGAVASSERNSIPAQEEDVYSSEQEEDPKNSAEHNAASQAASVPVPVPVPVPAPITSSVKQEKANQPLQAEAGETKKAPVPIFPASFPNFGGVESGLDKSPVAPQASSADMPRDSEEDQPKMSLKQRIAMLQEQQRLQQEREQELVQKKLEKKKKQERHEAAVIEASSTEALVIGEEQPEHPQEDSVPSTPEESAGPQLPSSTTVANDESTSVRRDTSEQEAAEGTQATIGGAEQERQLKDHETDDAESEGGHAAAEEEDEEARRAALRERMAKLAGAGRMGMPGSFNPFGIPIAPPEATAAKTKKPEETSKGDKEVSNLPRAVPVMPFADPNALPMFKNKALDTSEKEALSDSSEVPKESAGISDPRDEENDQGSGHVVEEVISKNGASKSAHEYAKLAAPPPPLDTPGMEADAEEEAGMVENNYFGKDARHTTVQPTQSETATPVKSQTPTQSPPIEMNRSNSKSDLASDSDGYLSSVEDISETGPKFPKDIPHLSKHAVKNAFSSTRSLSSGIESTGDGTEANLGQNKEGGFDVVKAAQDTRLDTATLNEYVPPHSNYLTNQHGEANIRAPPVPSAPPVPLRPSVDRVLPTDGDGERPSEAFGASTSREAPPVPTGGSAPPIPVEKRAPPVPPVGGVPPIPLEKSTPQIPSSRGAPQVPTDKGVPPIPTNKGAPPVPTGKQPLPIPSEPISRSTQHSATDRGHPDVPPVPSVPHAVNPIPSENLTESNSKIGSISSEADTPNQFASSTQKTPKEAPPPPPPIATKNENILEEGPKSPPPPPPPSVSQPSRRHNSVSSVGREKRNSGIPEVPRNIPPIPVATASHAPPSSAPSPSKSIPESFKNTEHSVPSSQAPSGPAGKEMKRSQTFEANLENQRPCIKFDAAEEWWLTKSIPAGLIGNNRLKYLWEMDENRIEKRGKEQWVMRDFYFLFEDYSQLHVTVTFNEANPQLSVKFWEEFIPRPSAGHDLEKFASKIGYKIFEQANQLINTSSRNFVPKLIEGSREPAIPPIASRTFGVPLLTYSPESTLDEDALKLIRPGDILAVRRGKFQSHGKLLQKTTHEIGMDAVPFAGVITEYDFSKHKFRVIEEHNGKIKQSSYRVHDMKSGKLKVYRVVGRSYVGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCH4</accession>
    <name>C5DCH4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">proteasome endopeptidase complex</fullName>
            <ecNumber evidence="3">3.4.25.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0B03102g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of peptide bonds with very broad specificity.</text>
            <dbReference type="EC" id="3.4.25.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.4.25.1" evidence="3"/>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551923.1">
        <property type="nucleotide sequence ID" value="XM_002551877.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCH4"/>
    <dbReference type="FunCoup" id="C5DCH4">
        <property type="interactions" value="1053"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCH4"/>
    <dbReference type="MEROPS" id="T01.010"/>
    <dbReference type="GeneID" id="8290756"/>
    <dbReference type="KEGG" id="lth:KLTH0B03102g"/>
    <dbReference type="eggNOG" id="KOG0174">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035750_5_2_1"/>
    <dbReference type="InParanoid" id="C5DCH4"/>
    <dbReference type="OMA" id="TFIYGYC"/>
    <dbReference type="OrthoDB" id="7854943at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010499">
        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03762">
        <property type="entry name" value="proteasome_beta_type_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000010">
        <property type="entry name" value="Proteasome subunit beta type-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000243">
        <property type="entry name" value="Pept_T1A_subB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF0">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE SUBUNIT HSLV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00141">
        <property type="entry name" value="PROTEASOME"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-0647">Proteasome</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0888">Threonine protease</keyword>
    <keyword evidence="9" id="KW-0865">Zymogen</keyword>
    <feature type="active site" description="Nucleophile" evidence="12">
        <location>
            <position position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00026071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR600243-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR21485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="215" mass="23570" checksum="81770A3B6BC05CDB" modified="2009-07-28" version="1">MNGIQVDINHLKKGEVSLGTSIMAVTFRDGVILGADSRTTTGAYIANRVTDKLTRVHEKVWCCRSGSAADTQAVADIVQHYLELYTAQFGEPSTKTAASIFKTLCYENKDNLTAGIIVAGYDEKNKGEVYSIPLGGSVHKQKYAIAGSGSTFIYGYCDKNYKENMSKDETVSFMKNSLSQAIKWDGSSGGVIRMVVLTKDGAERLIFYPEDYENL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCK5</accession>
    <name>C5DCK5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0B03806p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0B03806g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551954.1">
        <property type="nucleotide sequence ID" value="XM_002551908.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCK5"/>
    <dbReference type="FunCoup" id="C5DCK5">
        <property type="interactions" value="51"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCK5"/>
    <dbReference type="GeneID" id="8290788"/>
    <dbReference type="KEGG" id="lth:KLTH0B03806g"/>
    <dbReference type="eggNOG" id="ENOG502QTAU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043707_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCK5"/>
    <dbReference type="OrthoDB" id="3979966at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052058">
        <property type="entry name" value="Alcohol_O-acetyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010828">
        <property type="entry name" value="Atf2/Sli1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28037">
        <property type="entry name" value="ALCOHOL O-ACETYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28037:SF1">
        <property type="entry name" value="ALCOHOL O-ACETYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07247">
        <property type="entry name" value="AATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR21516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="522" mass="59496" checksum="15584E7DCCBA904B" modified="2009-07-28" version="1">MTTSQADTKLEELEKRGHARRLGNLENYFALGQRQDLYSNFGMFCELDRACSENELAEALRGMCLEYPLLLHTVLEKKEAQDVNFYQTSEYLSKPWPQHDYIRVLQRVRFADVLLNDQEEYAEIVNAALKEFASNGGQYSSEVFELINKVRIPYCHNSRPNWRIMCFPEEGNAQSREWRKILLLSNHCSSDGMSSANFFHDLQDHLNNLPPSLPQADVIFDYSQDHETLGKLPAPIETQISYVGPKSYFAQLVGNQVLREYFGYKSPTPPIPRVNEPGGNDFYSYFLKITPSEVAAVKKKLKNKLDPSCTLTPFFQACWFAALYKSGIVFSKSFSQQLSNIMVAMNTAQLLPEDKQLKKQYRYGANVGGSHYNYQISSFNVADKPEAFWKLVRYYQDVFVDAKRKKHFLYPLGALMIDSIYKTKNIDLAVTNSILGKSRLGTMLSNVGYFPQKARATVGGFHIQDLIFAQTTGSFRFTFDINLCATDIGGLNITACVAEGALPTREDWKKLCELFKTIILES</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCL1</accession>
    <name>C5DCL1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0B03938p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0B03938g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ribonucleoside diphosphate reductase small chain family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551960.1">
        <property type="nucleotide sequence ID" value="XM_002551914.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCL1"/>
    <dbReference type="FunCoup" id="C5DCL1">
        <property type="interactions" value="1455"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCL1"/>
    <dbReference type="GeneID" id="8290794"/>
    <dbReference type="KEGG" id="lth:KLTH0B03938g"/>
    <dbReference type="eggNOG" id="KOG1567">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035339_2_1_1"/>
    <dbReference type="InParanoid" id="C5DCL1"/>
    <dbReference type="OMA" id="SNPFPWM"/>
    <dbReference type="OrthoDB" id="10248373at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009263">
        <property type="term" value="P:deoxyribonucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01049">
        <property type="entry name" value="RNRR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.620.20">
        <property type="entry name" value="Ribonucleotide Reductase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012348">
        <property type="entry name" value="RNR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033909">
        <property type="entry name" value="RNR_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030475">
        <property type="entry name" value="RNR_small_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000358">
        <property type="entry name" value="RNR_small_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23409">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE SMALL CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23409:SF18">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE SUBUNIT M2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00268">
        <property type="entry name" value="Ribonuc_red_sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00368">
        <property type="entry name" value="RIBORED_SMALL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="403" mass="46123" checksum="D20BB1BC6E8FCB66" modified="2009-07-28" version="1">MVNAKETPSKAAASALSDLELKDSKNNLSGRLGAVPESAGEDAAALELKLSQAAEQHRQFLKSHHVHRHQLKEMEKEEPLLMENKRRFVLFPIKYHEIWQAYKRAEASFWTAEEIDLSKDLHDWNNRMNENERFFISRVIAFFAASDGIVNENLVENFSAEVQSPEAKCFYGFQIMMENIHSETYSLLIDTYIKDPKESDFLFNAIETIPQIKEKAEWAIRWIQDEDALFAERLVAFAAVEGVFFSGSFAAIFWLKKKGLMPGLTFSNELICRDEGLHTDFACLLFAHLKNKPDPAIIERIITEAVEIEQRYFRDALPVSLLGMNADLMNQYVEFVADRLLVALGNQKFYNVTNPFDFMENISLAGKTNFFEKRVSDYQKAGVMAKSTKAETEGGEFAFDEDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCN2</accession>
    <name>C5DCN2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">mRNA 3'-end-processing protein RNA14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0B04466g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the cleavage factor IA (CFIA) complex, which is involved in the endonucleolytic cleavage during polyadenylation-dependent pre-mRNA 3'-end formation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="4">Nucleus and/or cytoplasm.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551981.1">
        <property type="nucleotide sequence ID" value="XM_002551935.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCN2"/>
    <dbReference type="FunCoup" id="C5DCN2">
        <property type="interactions" value="1267"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCN2"/>
    <dbReference type="GeneID" id="8290818"/>
    <dbReference type="KEGG" id="lth:KLTH0B04466g"/>
    <dbReference type="eggNOG" id="KOG1914">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007630_0_1_1"/>
    <dbReference type="InParanoid" id="C5DCN2"/>
    <dbReference type="OMA" id="PKRQYFK"/>
    <dbReference type="OrthoDB" id="26282at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0180010">
        <property type="term" value="P:co-transcriptional mRNA 3'-end processing, cleavage and polyadenylation pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1660">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045243">
        <property type="entry name" value="Rna14-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008847">
        <property type="entry name" value="Suf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19980:SF0">
        <property type="entry name" value="CLEAVAGE STIMULATION FACTOR SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19980">
        <property type="entry name" value="RNA CLEAVAGE STIMULATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05843">
        <property type="entry name" value="Suf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="2 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Suppressor of forked" evidence="5">
        <location>
            <begin position="29"/>
            <end position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00026188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU369035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR21543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="654" mass="76637" checksum="00DCF2F6C7B43C8D" modified="2009-07-28" version="1">MSATPEIVKNGSAGFIRPTKRQIRPEEDVEGRLRDVIEERPGDICTYLELARLLQSRDQQEEARGVFDQLHERFPLFSPLWTVEVGYDLERDEFSHAGSLLTRCLSGTLENNDLGLWFMYLDFVRRKNNLITGGEEARGVVLKAFDAVATKCASWEPRSSAFWNEYLSFLEHWKPVSKWEEQQRIDLTRSLYKRMLCIPFDGLERSWNKYTQWEQEVNSLTARKFIGELSANYMKARSLYREWNNITKGIKRVLPSRLSQCSKQTIPQPGEYQVEQIHLWMDWIKWELQNKLDLPDEFHSQRVDYVYKQAIQHLLFSPEIWYNYSMYASPENAREILIQGLKASPGSTTLTFKLSEHFELQNEVEQMQKCFERCIDHLVLEYHIMSDEGQETYSQRQKITFVYCIYMNAMKRVSGLSSARKVFGKCRKLKDLLTHEIYIENAYLEFHNNNDHKTACKVLELGLKYFSSSGEYVNKYLDFLILINQDGLIKPLFESSLDRIIDLDELQRIYKKVINYESKFGNLTNAYSIENRFFEKFPQLEKIEVFTDRYQVQNANLIKSLELTYLPSDNDFRFGSFNETNGGLKRSRGLSQSGDSAAQKRHKSQDFVPENVLDLLRLLPKRQYFKTAVLDAEKLTRYLIENVNIPSSDNGTAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCU6</accession>
    <name>C5DCU6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">uracil phosphoribosyltransferase</fullName>
            <ecNumber evidence="4">2.4.2.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0B05918g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uracil: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UPRTase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.9" evidence="4"/>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552045.1">
        <property type="nucleotide sequence ID" value="XM_002551999.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCU6"/>
    <dbReference type="FunCoup" id="C5DCU6">
        <property type="interactions" value="775"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCU6"/>
    <dbReference type="GeneID" id="8290884"/>
    <dbReference type="KEGG" id="lth:KLTH0B05918g"/>
    <dbReference type="eggNOG" id="KOG4203">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067096_1_1_1"/>
    <dbReference type="InParanoid" id="C5DCU6"/>
    <dbReference type="OMA" id="KHKIGLM"/>
    <dbReference type="OrthoDB" id="106623at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004845">
        <property type="term" value="F:uracil phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008655">
        <property type="term" value="P:pyrimidine-containing compound salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000023">
        <property type="entry name" value="Probable uracil phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001097">
        <property type="entry name" value="PRK00129.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14681">
        <property type="entry name" value="UPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="10">
        <location>
            <begin position="13"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF14681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR21607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="216" mass="24450" checksum="4AB6C69B8396223D" modified="2009-07-28" version="1">MSTEPFKNVIMLPQTNQLTGLYTIIRDQNTKRPDFIFYADRIIRLLVEEGLNHLPVQPRTVETSTNQKYEGVSFLGKICGVSIVRAGESMEQGLRECCRSVRIGKILIQRDEETAQPKLFFEKLPEDIAERYVFLLDPMLATGGSAAMATEVLIKRGVKPERIFFLNLICSKEGVDYYHSKFPGVKIVTGAVDPCLDENKYLVPGLGDFGDRYYCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCV2</accession>
    <name>C5DCV2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0B06050p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0B06050g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552051.1">
        <property type="nucleotide sequence ID" value="XM_002552005.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCV2"/>
    <dbReference type="FunCoup" id="C5DCV2">
        <property type="interactions" value="24"/>
    </dbReference>
    <dbReference type="GeneID" id="8290890"/>
    <dbReference type="KEGG" id="lth:KLTH0B06050g"/>
    <dbReference type="eggNOG" id="ENOG502S2MY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116423_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCV2"/>
    <dbReference type="OMA" id="YQIKICW"/>
    <dbReference type="OrthoDB" id="4036106at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031501">
        <property type="term" value="C:mannosyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000030">
        <property type="term" value="F:mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019433">
        <property type="entry name" value="GPI_ManTrfase_II_coact_Pga1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28022">
        <property type="entry name" value="GPI MANNOSYLTRANSFERASE 2 SUBUNIT PGA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28022:SF1">
        <property type="entry name" value="GPI MANNOSYLTRANSFERASE 2 SUBUNIT PGA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10333">
        <property type="entry name" value="Pga1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950420" evidence="2">
        <location>
            <begin position="20"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR21613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="185" mass="20822" checksum="140B8CCE9DB2305D" modified="2009-07-28" version="1">MRIVPFFAALAFYICFSLANTESIVFKVPDTFSPAPQPIADENEPLISLEGTNRALKTFDAPVDSQFDIELRDLSPGDTFFAKICWTAVDPIDITSLGYSIKPDYTARGGHWQDAPLRFFLSFEAKSNSYPLFSKPTVPINISVSAMRMGVPVDLHATIIFMTLLTGLIFIINRHYKLYNRFKNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCW7</accession>
    <name>C5DCW7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">asparaginase</fullName>
            <ecNumber evidence="1">3.5.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0B06380g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.1.1" evidence="1"/>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552066.1">
        <property type="nucleotide sequence ID" value="XM_002552020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCW7"/>
    <dbReference type="FunCoup" id="C5DCW7">
        <property type="interactions" value="161"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCW7"/>
    <dbReference type="GeneID" id="8290905"/>
    <dbReference type="KEGG" id="lth:KLTH0B06380g"/>
    <dbReference type="eggNOG" id="KOG0503">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046466_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCW7"/>
    <dbReference type="OMA" id="KWFFDAS"/>
    <dbReference type="OrthoDB" id="4070114at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004067">
        <property type="term" value="F:asparaginase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009066">
        <property type="term" value="P:aspartate family amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036152">
        <property type="entry name" value="Asp/glu_Ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006034">
        <property type="entry name" value="Asparaginase/glutaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027473">
        <property type="entry name" value="L-asparaginase_C"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001220">
        <property type="entry name" value="L-ASNase_gatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500176">
        <property type="entry name" value="L_ASNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00870">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53774">
        <property type="entry name" value="Glutaminase/Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51732">
        <property type="entry name" value="ASN_GLN_ASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950421" description="asparaginase" evidence="2">
        <location>
            <begin position="17"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR21628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="345" mass="35385" checksum="947ED6BC4AB13A90" modified="2009-07-28" version="1">MRASLILSAICAAGAASPIAAVGDDTTYVVEAGPASLTPWNVWPSWSNASNVSNYSNLSNATAQVAAPKLEVIVTGGQYTMSNWSSEVHVTRLFNQSTLNATQLARVAQSVEGALDGGEYTGVVVLGAGRALEALGFYLSVVVDTPENVVVASALEDGVATAWSAWSWFRGALVVDGGVIYSGAAYSPSAPGWGALGSVIAGAPRYWFTPAWPTLLAPWGSLRVNFTNFTDIVPAPAANATNSSSSMTVPVVFDGYYDPSIVSSLSTAIAGLVVVSSGNSTASDLTSSNIPVVFTSDDGATILPEDVPAGAIAGGDLSPLKAQLLLSIALSKDVKETSSLQQLFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>C5DD52</accession>
    <name>C5DD52_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein URE2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0B08360g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Plays an important role in the cellular response to the nitrogen source.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the GST superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552151.1">
        <property type="nucleotide sequence ID" value="XM_002552105.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DD52"/>
    <dbReference type="FunCoup" id="C5DD52">
        <property type="interactions" value="766"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DD52"/>
    <dbReference type="GeneID" id="8290991"/>
    <dbReference type="KEGG" id="lth:KLTH0B08360g"/>
    <dbReference type="eggNOG" id="KOG0867">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011226_14_1_1"/>
    <dbReference type="InParanoid" id="C5DD52"/>
    <dbReference type="OMA" id="YEPHRID"/>
    <dbReference type="OrthoDB" id="422574at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042128">
        <property type="term" value="P:nitrate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006808">
        <property type="term" value="P:regulation of nitrogen utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10293">
        <property type="entry name" value="GST_C_Ure2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03048">
        <property type="entry name" value="GST_N_Ure2p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000034">
        <property type="entry name" value="Transcriptional regulator URE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017298">
        <property type="entry name" value="Ure2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051:SF3">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR URE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037861">
        <property type="entry name" value="Prion_URE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 3" id="KW-0534">Nitrate assimilation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="6">
        <location>
            <begin position="117"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="7">
        <location>
            <begin position="210"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="51"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>glutathione</name>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>glutathione</name>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="169"/>
            <end position="170"/>
        </location>
        <ligand>
            <name>glutathione</name>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="185"/>
            <end position="186"/>
        </location>
        <ligand>
            <name>glutathione</name>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR037861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR037861-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR21713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="359" mass="40922" checksum="6F8571645F36FDF2" modified="2009-07-28" version="1">MVGEKQTSVGGSVSAAVSNLSSALRQVNLGNSNTTTDQSNINIDFHNRMNRQQLMGEAQRNNLNAQQAEELLRQQEAHQQQQLQQAQQQQQQQQQYVGPAQIDSSRISKFFQNQPEEGYTIFSHRSAPNGFKVSIVLSELNLQYNTIFLDFNIGEHRAPDFVAINPNARVPALIDHSMDNLAIWESGAIILHLVNRYYRETGEPLLWSENMGEQAHITAWLFFQTSGHAPMIGQALHFRYFHSQKIQSAVDRYTDEVRRVYGVVEMALAERREALIMELDTENAAAYSAGTTPLSRSRFFDYPVWLVGDKITVADLSFVPWNNVVDRIGINIKLEFPEVYKWTKHMMRRPAVIKALRGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5DD70</accession>
    <name>C5DD70_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0B08800p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0B08800g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat RAPTOR family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552169.1">
        <property type="nucleotide sequence ID" value="XM_002552123.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DD70">
        <property type="interactions" value="917"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DD70"/>
    <dbReference type="GeneID" id="8291011"/>
    <dbReference type="KEGG" id="lth:KLTH0B08800g"/>
    <dbReference type="eggNOG" id="KOG1517">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001136_0_2_1"/>
    <dbReference type="InParanoid" id="C5DD70"/>
    <dbReference type="OMA" id="TEVCTND"/>
    <dbReference type="OrthoDB" id="10262360at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031931">
        <property type="term" value="C:TORC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071230">
        <property type="term" value="P:cellular response to amino acid stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030307">
        <property type="term" value="P:positive regulation of cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031929">
        <property type="term" value="P:TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004083">
        <property type="entry name" value="Raptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029347">
        <property type="entry name" value="Raptor_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12848">
        <property type="entry name" value="REGULATORY-ASSOCIATED PROTEIN OF MTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12848:SF16">
        <property type="entry name" value="REGULATORY-ASSOCIATED PROTEIN OF MTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14538">
        <property type="entry name" value="Raptor_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01547">
        <property type="entry name" value="YEAST176DUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM01302">
        <property type="entry name" value="Raptor_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Raptor N-terminal CASPase-like" evidence="6">
        <location>
            <begin position="79"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="322"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1052"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="804"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1060"/>
            <end position="1071"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR21731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1515" mass="171204" checksum="B62C7EEA83E550CA" modified="2009-07-28" version="1">MPTTYGPQPLRKLNTETRHGFGDQYSSEQYLENLENDFMFYFDDKRHRTNGNPTSPEVKAKDESHYYQPIPDWKIVKERQKTVSAALLLCLNLGVDPPDVAKTHPCARLESWVDPLNFQDSKKAIEQIGKNLQSQYETLSLRTRYKQSLDPCVEDVKRFCNSLRRASKDERILFHYNGHGVPQPTASGEMWVFNRGYTQYIPVSLYDLQTWLGAPCIYVYDCNSAGNIVANFQTFVQKRIKDDQDGNHDPAAPSPTQAYTECIQLASCRANELLPMKPELPADLFTCCLTCPIKISVEIFLMQSPLRESKYSVLFQDYDSRTHRSGEQSSSAKPNVTIPGKLSDRRTPLGELNWIFTAITDTIAWTSLPRPLFKKLFRHDLMVAALFRNFLLAKRIMPWFNCHPVSDPELPDSIADHPMWKSWDLAIDEVLTKLVDDLQNVPLASADLELQPSLQEQQVSDTSIDDPLHSKAAAGTLSTMSLANHQAGSLSQVSQHLSQQQLLQHQHQQQQLQQQQQQQQFTGFFEQNLTAFELWLKYGSNTRHPPEQLPIVLQVLLSQVHRVRALVLLSRFLDLGPWAVYLSLSIGIFPYVLKLLQSPAPELRPILVFIWARIMSIDYKNTQAELIKEKGYLYFVKILVPELGMHPQPGSAPQLQRTISGGGLTMNASTSIGTQRYQFYQNFQNANNPGVAMANNTTDEQKAMAMFVLSSFLRDFSLGQKYCSSLELLGKICYYVETSDVPLLRQWCIVLMGQLYLKNPFNKYLCMTCGFLDKIYTSMKDPIPEIRCAALLAFSMFISEKNDAEMALQMQQEMQQQAQQLQVQLQTLQGGQMQRQQAQAERQLQQLQASLSQMRTMDSRKLKQDEIKMTIEALGLVNDGSPLVRKELIISLSRIVHRYINFFFVIAFNELCDEISQLESTGDSAALESKQSIAHGSIFNTVWKSLLILAEDPYLENKTLAQQVVDYVFTKLNVHSQLNEVVLLMELYLVKHTSCNATGHGATTVSILPTAKRPLSLSANKREATSNSETPKSGIFKRSISLNGLLKALGWEQDQEDSSQETPSNRSFGRLQGNNLLTVPYGVSPSATTPRYKKPKGPIDMPLKSSFLDYSREYFQEPQMKKQEADEPGSVEYTSRLWRRNRNESIIQRTQAQKRLALYGDWSNKIGTLDNKTQPKLLKFSQFENYIACSDDRDNISIFDWVDKSKLSTFSNGNPFGTKITDMSYLNEDDVPLLLAGSSDGIVKIYKNFQDLEELELVSSWRGLTDLLLTPRSTGLLTEWQQSRGSLLVTGDAKIIRVWDALTETIEVDIPAKSSSLITSITSDQLSGHMFVTGFSDGSLRAYDRRVDPRDSMVRLWRSGDGKSNFGINNIHLQRGGYRELVSGTENGLVELWDLRYQNSIGKFKDEGDKSSSMTTMTSMKVHEHAPVIATGTKSLKIWTTSGDLLSSFKNNTPAHAGGVAGTLATGISASRISSSFISTMAFHPHEMILAAANSHNSSINIYECYEGKTDYLYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDB8</accession>
    <name>C5DDB8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pre-mRNA-splicing factor CWC22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0B09900g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CWC22 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552217.1">
        <property type="nucleotide sequence ID" value="XM_002552171.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDB8"/>
    <dbReference type="FunCoup" id="C5DDB8">
        <property type="interactions" value="976"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDB8"/>
    <dbReference type="GeneID" id="8291061"/>
    <dbReference type="KEGG" id="lth:KLTH0B09900g"/>
    <dbReference type="eggNOG" id="KOG2140">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006308_3_4_1"/>
    <dbReference type="InParanoid" id="C5DDB8"/>
    <dbReference type="OMA" id="ILTEDMR"/>
    <dbReference type="OrthoDB" id="3938623at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050781">
        <property type="entry name" value="CWC22_splicing_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003891">
        <property type="entry name" value="Initiation_fac_eIF4g_MI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003890">
        <property type="entry name" value="MIF4G-like_typ-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18034">
        <property type="entry name" value="CELL CYCLE CONTROL PROTEIN CWF22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18034:SF3">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR CWC22 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02847">
        <property type="entry name" value="MA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00544">
        <property type="entry name" value="MA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00543">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51366">
        <property type="entry name" value="MI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MI" evidence="8">
        <location>
            <begin position="286"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="497"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="497"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="521"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="557"/>
            <end position="588"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR21779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="596" mass="68519" checksum="894D3140632100B1" modified="2009-07-28" version="1">MSQEDQDVVILQKESWDHLKSYIKQCFRSLTRSSVVEEYRKLFAVNILWGERLVVSSVLQKQSAGDSSEILAALTSLLNAHIPQLGELLIEETTSRFLNAFKTHNSKGCYAMAALIAELFNYEVLHEILILQILHLLMEGVNVDSACLAMFVLSKCGRQLMLVAKTAHDLAFESLRKFFQENKVERAVYRRFEDLFDLRKRNYPQLSKRIHLPDHETVTHTFLLDLESSTPSSDESLEEFRYDRNFMEKESEIETLRSKLFALEGTDATPDEQRPAEDKTQSRELEMKKKIYLTLKGSLSGDEAAHKILKYKFPDSEKKAVVDILVTACSQETTYSKFYGIVAERLCSSHKSWKAAFGQSFHDNYSAVDEFQANQVRNMGKFWGHILASDYLGFEVFESVHMNANETSSAGRVYLKFLFQELVLDLSIDTLQARLDEPYIQPFLVNLFPKDDAEKSRFSINYFTAIGLGRLTEGMRQALADMEKKVVALQDTEAESGSSLFSRRGSSSSPKTKAYIQGERASSETDNSHSLERSYRRPQDEEFSQTRYSESLTRSRTSVRRRSRTPTRRRSRTPTRRRSRTPARRRSRSPNTRFGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDD9</accession>
    <name>C5DDD9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0B10494p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0B10494g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552238.1">
        <property type="nucleotide sequence ID" value="XM_002552192.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDD9"/>
    <dbReference type="GeneID" id="8291088"/>
    <dbReference type="KEGG" id="lth:KLTH0B10494g"/>
    <dbReference type="eggNOG" id="ENOG502QTSQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018249_1_0_1"/>
    <dbReference type="InParanoid" id="C5DDD9"/>
    <dbReference type="OMA" id="HAYMDSG"/>
    <dbReference type="OrthoDB" id="5427350at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053143">
        <property type="entry name" value="Arylsulfate_ST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039535">
        <property type="entry name" value="ASST-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35340:SF9">
        <property type="entry name" value="ASST-DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35340">
        <property type="entry name" value="PQQ ENZYME REPEAT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14269">
        <property type="entry name" value="Arylsulfotran_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950428" evidence="1">
        <location>
            <begin position="19"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="533" mass="59990" checksum="E06E081C555A02AF" modified="2009-07-28" version="1">MKLKCFCTISLAASFVAADWQFKSRPDLSPPILNIAIPANQNTSPGYLFAAPFSGYADNKNHGPRQAAPYIFTDKGELVWSGFSYFSIWATNFQKGRLNGEDILFSFEGSHNPDYGHGHGHVTILDSHYQTIKELRAGRHKIIDKHEFHIIDEKTGLVQIYQPVPRDLTLYAKSEEQQWIVDAIFQEIDIESGEVLFEWRSLDWISPDESILPINEGQAGSGFNSSDAWDYFHINSVDKNEEGDYLLSARDAAALYKINGTSGEVIWKLGGLPELSSDFKIDFQFSFQHHARFISRNGSKEVISFYDNSAHGTENKDGKTVSYAETSSGKVIEVDNKEFTAHLLREFLPPDGLLSKSQGSTQVLSDGHVIVNWGSKGAVTEYNSKDEAVFHAYFDSDLLGENVENYRAFKFNWIGEPDEDISLYSEISADGEASIYVSWNGDTKTQKWRFFELDEEGEKQIIGATTRKGFETAFKTNKIKLSNKVLVEALGERGEVLGVSDPTLSQKEIIPAGGKKVSTKNSFSNYFEWRMWQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-04-02" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDU9</accession>
    <name>C5DDU9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0C03960p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0C03960g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552398.1">
        <property type="nucleotide sequence ID" value="XM_002552352.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDU9"/>
    <dbReference type="GeneID" id="8291262"/>
    <dbReference type="KEGG" id="lth:KLTH0C03960g"/>
    <dbReference type="eggNOG" id="ENOG502SC3B">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1245567_0_0_1"/>
    <dbReference type="InParanoid" id="C5DDU9"/>
    <dbReference type="OrthoDB" id="10436976at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="165"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="26"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="206"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="222" mass="23141" checksum="F2D5E402E6C49FE3" modified="2009-07-28" version="1">MAKTLAQGRKPGSGRKPGKGKTLAQGRKPGSGRKPGSKGQVTKKSAAANAMALAAEPVGPVELSLSESPGAPQIQKLNQVNRLPSVHDDFTHVTGDLRAHSSRIPSIEMFHAGRMAHMGATPPASSPPPPPLPLQLQPSMSSFLPLQPWAASAAPSSLLPGFPAFMPPTLPQQQPATRPGAMQGSSLDPMRATKLFATDRFHGSPDPDSLSSFASSSHSSRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>C5DE68</accession>
    <name>C5DE68_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ribose-phosphate diphosphokinase</fullName>
            <ecNumber evidence="4">2.7.6.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0C06710g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">5-phosphoribose 1-diphosphate synthase involved in nucleotide, histidine, and tryptophan biosynthesis. Active in heteromultimeric complexes with other 5-phosphoribose 1-diphosphate synthases (PRS2, PRS3, PRS4 and PRS5).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>D-ribose 5-phosphate + ATP = 5-phospho-alpha-D-ribose 1-diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15609"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ribose-phosphate pyrophosphokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.1" evidence="4"/>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552517.1">
        <property type="nucleotide sequence ID" value="XM_002552471.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DE68"/>
    <dbReference type="FunCoup" id="C5DE68">
        <property type="interactions" value="575"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DE68"/>
    <dbReference type="GeneID" id="8291385"/>
    <dbReference type="KEGG" id="lth:KLTH0C06710g"/>
    <dbReference type="eggNOG" id="KOG1448">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033546_4_0_1"/>
    <dbReference type="InParanoid" id="C5DE68"/>
    <dbReference type="OMA" id="IHNCESI"/>
    <dbReference type="OrthoDB" id="413572at2759"/>
    <dbReference type="UniPathway" id="UPA00087">
        <property type="reaction ID" value="UER00172"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002189">
        <property type="term" value="C:ribose phosphate diphosphokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004749">
        <property type="term" value="F:ribose phosphate diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006015">
        <property type="term" value="P:5-phosphoribose 1-diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009156">
        <property type="term" value="P:ribonucleoside monophosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000031">
        <property type="entry name" value="Probable PRS4-ribose-phosphate pyrophosphokinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000005">
        <property type="entry name" value="Ribose-phosphate pyrophosphokinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000842">
        <property type="entry name" value="PRib_PP_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029099">
        <property type="entry name" value="Pribosyltran_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005946">
        <property type="entry name" value="Rib-P_diPkinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002320">
        <property type="entry name" value="PRK01259.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01251">
        <property type="entry name" value="ribP_PPkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210">
        <property type="entry name" value="RIBOSE-PHOSPHATE DIPHOSPHOKINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210:SF32">
        <property type="entry name" value="RIBOSE-PHOSPHATE PYROPHOSPHOKINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14572">
        <property type="entry name" value="Pribosyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13793">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01400">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00114">
        <property type="entry name" value="PRPP_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribose-phosphate pyrophosphokinase N-terminal" evidence="13">
        <location>
            <begin position="25"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF13793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR22079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="337" mass="36828" checksum="5426B8220325ACCE" modified="2009-07-28" version="1">MSSGGDKNLLDIDAPKPFWMSSNSIKLLSGNSHPELAEKIARVLGLSLSKIGVYQYSNKETSVTIGESIRDEDVYIIQTGSGQQEINDFLMELLIMIHACKTASARRITAVIPNFPYARQDKKDKSRAPITAKLVANLLETAGCDHVITMDLHASQIQGFFHIPVDNLYAEPSALNYIKTTTDIENAILVSPDAGGAKRVASIADKLDLNFALIHKERQKANEISRMVLVGDVRGKSCILIDDMADTCGTLVKASDMLFDNGAKEVVALVTHGIFSGSAREKLKNSKLSRIVCTNTMPVDLQLDIFDQIDISPTLAEAIRRLHNGESVSYLFTHAPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-04-02" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEG6</accession>
    <name>C5DEG6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0C08998p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0C08998g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552615.1">
        <property type="nucleotide sequence ID" value="XM_002552569.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEG6"/>
    <dbReference type="GeneID" id="8291489"/>
    <dbReference type="KEGG" id="lth:KLTH0C08998g"/>
    <dbReference type="eggNOG" id="ENOG502QSZT">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038732_9_0_1"/>
    <dbReference type="InParanoid" id="C5DEG6"/>
    <dbReference type="OMA" id="ISIWVQV"/>
    <dbReference type="OrthoDB" id="2349068at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018580">
        <property type="term" value="F:nitronate monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04730">
        <property type="entry name" value="NPD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004136">
        <property type="entry name" value="NMO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32332">
        <property type="entry name" value="2-NITROPROPANE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32332:SF34">
        <property type="entry name" value="2-NITROPROPANE DIOXYGENASE FAMILY, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03060">
        <property type="entry name" value="NMO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51412">
        <property type="entry name" value="Inosine monophosphate dehydrogenase (IMPDH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2" id="KW-0288">FMN</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR22177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="353" mass="37294" checksum="7F215108327BE0DF" modified="2009-07-28" version="1">MSSSLRSIYPWIRSPLVACSPMLRISQPALAVAVSHAGGLGFVAGGYDTDELALNMKNAVGLLRGLDPPVPTQTGVLPIGIGFVLWGCDLERSVQVIQQFVPAAVWLFAAPSNEQYALWASRIRDATQSRTQIWIQVAGVKEGLDVVRAAQPDVLVAQGNDAGGHGMKRSASVISLVPELRDSLDAHGFGRTTVLAAGGIVDGRGAAAALVLGADGVVMGTRFLASHEAVVAKGYQQELLRAEDGGVSTVRSRLFDRVKETNGWPDDYDGRAFVNRTFLDAQNGMPVEQNISLHKQEVAHGDTDGGVKARTIRYAGTGVGLIHDVVPAAEIVNTVAAEVEMSLAKAIKKLNAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEH7</accession>
    <name>C5DEH7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0C09328p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0C09328g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552626.1">
        <property type="nucleotide sequence ID" value="XM_002552580.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEH7"/>
    <dbReference type="GeneID" id="8291504"/>
    <dbReference type="KEGG" id="lth:KLTH0C09328g"/>
    <dbReference type="eggNOG" id="ENOG502S4WZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070629_0_0_1"/>
    <dbReference type="InParanoid" id="C5DEH7"/>
    <dbReference type="OMA" id="CIIPRIC"/>
    <dbReference type="OrthoDB" id="4038005at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR22188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="330" mass="37654" checksum="58B44B750E8C8542" modified="2009-07-28" version="1">MNDSTYRVSFSEDSDDSQDDTVVPFITLVRNRNVLPPSEFVDSIANDNLKNCHNLVFLLWLLNSPVHLNHEFLRSDRPFLLFSNCYSASGSHNYEEYLQIWPRFAKTGGDSADSFEVSVFPQALQFTKLDQFRMDKLTHQYTQQFGRILNSYSLQSSQQSSHYLRANLHALLQSYVYDTLFDLFFKWKSWSQITGRGFGLVSFSSLVGKFWDSYMCLQFDDSLQSLASTLNSKSDCKNFTQQPETDFTGCKSWRIRYAVWVDTANGIMLLINGVNGGNIGVVPAQGLDLTPRNVEQNISKLLLFVNMCLIECLSWELNGKTETGLYIKGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEJ4</accession>
    <name>C5DEJ4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tryptophan--tRNA ligase, cytoplasmic</fullName>
            <ecNumber evidence="3">6.1.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Tryptophanyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0C09768g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.2" evidence="3"/>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552643.1">
        <property type="nucleotide sequence ID" value="XM_002552597.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEJ4"/>
    <dbReference type="FunCoup" id="C5DEJ4">
        <property type="interactions" value="1118"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEJ4"/>
    <dbReference type="GeneID" id="8291523"/>
    <dbReference type="KEGG" id="lth:KLTH0C09768g"/>
    <dbReference type="eggNOG" id="KOG2145">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032621_0_1_1"/>
    <dbReference type="InParanoid" id="C5DEJ4"/>
    <dbReference type="OMA" id="SIYHRFM"/>
    <dbReference type="OrthoDB" id="10261385at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004830">
        <property type="term" value="F:tryptophan-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006436">
        <property type="term" value="P:tryptophanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00806">
        <property type="entry name" value="TrpRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.240.10:FF:000003">
        <property type="entry name" value="Tryptophan--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000033">
        <property type="entry name" value="tryptophan--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.240.10">
        <property type="entry name" value="Tyrosyl-Transfer RNA Synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002306">
        <property type="entry name" value="Trp-tRNA-ligase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00233">
        <property type="entry name" value="trpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10055:SF1">
        <property type="entry name" value="TRYPTOPHAN--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10055">
        <property type="entry name" value="TRYPTOPHANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01039">
        <property type="entry name" value="TRNASYNTHTRP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR22205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="426" mass="48416" checksum="08B94AF7267C3010" modified="2009-07-28" version="1">MTEQISPAAASEKLADLSVKEQNITPWDVEGAVDENGQSQDIDYDKLIKQFGTKAVGEETLQRFEQVTGHKPHHFLRKGLFFSERDFNKILDLYEQGKPFFLYTGRGPSSDSMHLGHMVPFVFTKWLQDVFDAPLVIELTDDEKFLFKQKLTIEDVKRFARQNASDIISVGFNPQKTFIFSDLAYMGGGFYETVVRVSRQITGSTAKAVFGFNDSDCIGKFHFASIQIASAFPSSYPDVLGIPNKTPCLIPCAIDQDPYFRVCRDVAEKLKFSKPALIHSKFFPALQGSTTKMSASDDTTAIFMTDTPKQIQKKINKYAFSGGQVSIEDHRKLGGNPDVDVAFQYLSFFKDDDEFLEDVYNKYKSGELLSGEMKKLCIDVLQEFVKEFQERKARVTDEVIDQFMQPHKLVWGQQERLVPVKPKEKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-09-22" modified="2025-06-18" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEL5</accession>
    <name>SEY1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein SEY1</fullName>
            <ecNumber evidence="1">3.6.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">SEY1</name>
        <name type="ordered locus">KLTH0C10230g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Cooperates with the reticulon proteins and tubule-shaping DP1 family proteins to generate and maintain the structure of the tubular endoplasmic reticulum network. Has GTPase activity, which is required for its function in ER organization.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <text evidence="1">Enriched in the cortical ER. Concentrated in punctae along the ER tubules.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class dynamin-like GTPase superfamily. GB1/RHD3 GTPase family. RHD3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.-" evidence="1"/>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552664.1">
        <property type="nucleotide sequence ID" value="XM_002552618.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEL5"/>
    <dbReference type="SMR" id="C5DEL5"/>
    <dbReference type="FunCoup" id="C5DEL5">
        <property type="interactions" value="68"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEL5"/>
    <dbReference type="GeneID" id="8291544"/>
    <dbReference type="KEGG" id="lth:KLTH0C10230g"/>
    <dbReference type="eggNOG" id="KOG2203">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011270_0_0_1"/>
    <dbReference type="InParanoid" id="C5DEL5"/>
    <dbReference type="OMA" id="PIIKMTE"/>
    <dbReference type="OrthoDB" id="1597724at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016320">
        <property type="term" value="P:endoplasmic reticulum membrane fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01851">
        <property type="entry name" value="GBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000727">
        <property type="entry name" value="Protein SEY1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03109">
        <property type="entry name" value="Sey1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030386">
        <property type="entry name" value="G_GB1_RHD3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008803">
        <property type="entry name" value="RHD3/Sey1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046758">
        <property type="entry name" value="Sey1/RHD3-like_3HB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45923">
        <property type="entry name" value="PROTEIN SEY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45923:SF2">
        <property type="entry name" value="PROTEIN SEY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05879">
        <property type="entry name" value="RHD3_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20428">
        <property type="entry name" value="Sey1_3HB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51715">
        <property type="entry name" value="G_GB1_RHD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000384984" description="Protein SEY1">
        <location>
            <begin position="1"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="1">
        <location>
            <begin position="1"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="680"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="topological domain" description="Lumenal" evidence="1">
        <location>
            <begin position="701"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="704"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="1">
        <location>
            <begin position="725"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="domain" description="GB1/RHD3-type G" evidence="2">
        <location>
            <begin position="35"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="752"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="45"/>
            <end position="52"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="783" mass="89504" checksum="8E9FD17E8B38D49A" modified="2009-07-28" version="1">MGETEEAIQLINEEKEFNSSTLQYFQKCVKERDVGVDYHVISVFGSQSSGKSTLLNALFHTQFDTMNAKVKRQQTTKGIWLGHTTRIATLENSLTPVKDLFVLDVEGSDGAERGEDQDFERKAALFAIAISEVLIVNMWEQQVGLYQGNNMALLKTVFEVNLSLFGHQNDHKVLLLFVIRDHVGVTPLSSLQETLEAELNAIWSDLAKPEGCENSSLYDFFDLKFTALAHKVLQPDLFMENVKELGNKFSSETPGKSFFKPEYHHNLPLDGWTMYAGNCWNQIETNKDLDLPTQQILVAKFKTEEISNQALALLLENYPVDLHCRELSDLAGKLQELKSLCLQEYDTYASRYAKPVYMDNRRDLSHKIENKFKETISKFLDVQKEDLLSQVEKDLTERSRSTPFVTKMKEARKKAFDAFEELLLQFTKLELVASLSDENEAFSIALDEACSNQSRKQLKQSVNRISKSVSSGIKEDIFFLLAHPELDLWDKVMEKFNEIVSDSTERYAVDDEKFDFQVGLSEEENQKVYKKVRSNAWTNLYEFVHGYLNEDNVVSILRDRFENKFRYDEHDSPRLWRNEEEIDNAFRVAKEHALEVLNVLSLASNSDHVEIVPDVPLSSGLDDEELEGEYEDEEGIYHEKGFAHILTASQRERAVQKFKRQANTTVIEAKRSIIRTTTHIPLYVYALLVVLGWNEFMIVIRNPLFVTLLVILSVTAYFVHKLNLWAPILALVNSAVSETKALARNKLKVILDDDSSSSSKASKEPTEEYEMQDLAKSSTTGTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>C5DF25</accession>
    <name>C5DF25_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">DNA polymerase</fullName>
            <ecNumber evidence="20">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="ordered locus">KLTH0D11682g</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="28">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18 20">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 20">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="19">Forms DNA polymerase zeta with REV7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 20">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 20">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="20"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553218.1">
        <property type="nucleotide sequence ID" value="XM_002553172.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DF25">
        <property type="interactions" value="725"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DF25"/>
    <dbReference type="GeneID" id="8295458"/>
    <dbReference type="KEGG" id="lth:KLTH0D11682g"/>
    <dbReference type="eggNOG" id="KOG0968">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000203_3_1_1"/>
    <dbReference type="InParanoid" id="C5DF25"/>
    <dbReference type="OMA" id="GRNKMGF"/>
    <dbReference type="OrthoDB" id="2414538at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016035">
        <property type="term" value="C:zeta DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:nucleotide binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042276">
        <property type="term" value="P:error-prone translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05778">
        <property type="entry name" value="DNA_polB_zeta_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05534">
        <property type="entry name" value="POLBc_zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.60:FF:000007">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.690:FF:000002">
        <property type="entry name" value="DNA polymerase zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.60">
        <property type="entry name" value="DNA polymerase family B, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.342.10">
        <property type="entry name" value="DNA Polymerase, chain B, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.690">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017964">
        <property type="entry name" value="DNA-dir_DNA_pol_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006133">
        <property type="entry name" value="DNA-dir_DNA_pol_B_exonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006134">
        <property type="entry name" value="DNA-dir_DNA_pol_B_multi_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042087">
        <property type="entry name" value="DNA_pol_B_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056435">
        <property type="entry name" value="DPOD/Z_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030559">
        <property type="entry name" value="PolZ_Rev3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056447">
        <property type="entry name" value="REV3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025687">
        <property type="entry name" value="Znf-C4pol"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45812">
        <property type="entry name" value="DNA POLYMERASE ZETA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45812:SF1">
        <property type="entry name" value="DNA POLYMERASE ZETA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00136">
        <property type="entry name" value="DNA_pol_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03104">
        <property type="entry name" value="DNA_pol_B_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24055">
        <property type="entry name" value="POL3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24065">
        <property type="entry name" value="REV3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14260">
        <property type="entry name" value="zf-C4pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00106">
        <property type="entry name" value="DNAPOLB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00116">
        <property type="entry name" value="DNA_POLYMERASE_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 20" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="16" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7 20" id="KW-0235">DNA replication</keyword>
    <keyword evidence="15 20" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="12 20" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="13 20" id="KW-0408">Iron</keyword>
    <keyword evidence="14 20" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8 20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 20" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="17 20" id="KW-0539">Nucleus</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="11 20" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 20" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DNA polymerase zeta catalytic subunit N-terminal" evidence="26">
        <location>
            <begin position="20"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="DNA polymerase delta/zeta catalytic subunit N-terminal" evidence="25">
        <location>
            <begin position="73"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B exonuclease" evidence="23">
        <location>
            <begin position="660"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B multifunctional" evidence="22">
        <location>
            <begin position="910"/>
            <end position="1360"/>
        </location>
    </feature>
    <feature type="domain" description="C4-type zinc-finger of DNA polymerase delta" evidence="24">
        <location>
            <begin position="1395"/>
            <end position="1475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="410"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="410"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="435"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="462"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
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            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
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            <dbReference type="ARBA" id="ARBA00023242"/>
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    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00066055"/>
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    <evidence type="ECO:0000256" key="20">
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            <dbReference type="RuleBase" id="RU000442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF00136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
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            <dbReference type="Pfam" id="PF03104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF14260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF24055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="Pfam" id="PF24065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EMBL" id="CAR22780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1500" mass="171399" checksum="A774178F331B76F0" modified="2009-07-28" version="1">MSSGAPESGSFSTLAEDLIHIQINSYDSYQEVPGAFDRLSGNSLPNRQFNHVPVIRVYGSLPSGHNVLCHIHGAFPYIYVPYDGEKGDSAQTIQSRCTQLHSCLETKLKESYNRKGSAEEEEGKEKEKKDGFPENLKFVADVSPIRAVPFYGYHVGYSVFYKISLLSSATTTKTAEFLREGKATGKRSQVFEAHIPFLLQFSADYNLFGCSWLKLSKCFFRSPLLNPAHSQDLLQRNESLDEFLGRFYRKGENESGDDCERIGNSLLEIDAVPQFINNRNEIDFRDIHGTLFESSQIGPDETLFIPSTKNLWTQTTHLRKQFGLDPYQPIPEQHRFSRLPEWQSHKELLSFLQKAKDRTIPKSKSFSESVSKSMFSAARHLDLPHESLCQLWPDSHPRVGLDLSHRVQNQNTNRESFNSDSTSNDIVYSSDSETASEREMEMEQFHNTENTKEGKKTETEARLQSSAPLNPQKDTGAVDSFNDQILTQSMAHKRPSKGEGLLNQRPKKLIRPVYAQSISAPSSGFRYKKLSLEFYNILQDLEDRGIPKIDYMDPYFRSPEDLKKKDYIYSGRKFSLNSTHVTCRRPIEFANRTVRLESTSDERQPKNSWKYAIAPPTYSTVHDYILHTCKVNYRSQIDFKTMSNERGYKFKSNNSLKGLNSGDHNPLSHLTLEIFATSKANRLPDPNFDPVQLIFWKIDPDSFPIDIGISTEGIMAYHEKDDAEFISHLNSASWSTPVAFFQSEEEMINGVADLVLLLDPDIISGFELHSSSWGYLIERLESAYERDLCEEIARVKYKTFNKRKDVWGFSHDSGIHIAGRHTLNIWRLLRSGLNLLKYTLENITFHVLHERLPHFSPSALTSLWESRSNITALTTLISYWKKRVNINMKLLHSQQTINQTIEQARLIGIDFYSVISRGSQYKVESVLMRLCKCEHFLVLSPSKTQVRDQKALECIPLVMEPESAFYKSPLVVLDFQSLYPSIMIGYNYCYSTIVGTVHEYNSKKTAIGASKVKFARGLLHELEDHLTISPNGVMYLKESVRKSMLAKMLTDILHTRLMIKATMSDLKSKNETVAKLLNNKQVALKLLANVTYGYTSASFSGRMPCSDVADSIVQTGRETLEKAISLIESHSQWGAKVVYGDTDSLFVYFPGKSREEAFKFGKEMAKAITTCNPFPITLKFEKVYHPCLLVSKKRYVGYSFEKEDQNEPKFDAKGIETVRRDGHPAQQKIVEKSLRILFDSKDLSAVKSYVQDQFRKIMKGQVSVQDFCFAREVKLGSYKSEKTAPPGAVVAAKKKAADHRAGPQYRERIPYVVVKGRPGQILRERCVPPEMFLSNDSLELDATYYITKTLLPPLQRFFNLIGVDVSQWYVEMPRFQDIGNYQDGNLPEFMRTTSCFNCKKEIREKTKDLCAECFENKSQTITNLLQEDVSIRKHFKSILTVCRACCQPFTKNSLDNLNKIVTQCDAQDCPVYYSRVKYKGYMSTARSAQLRDILKGLDHW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>C5DF53</accession>
    <name>C5DF53_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0D12298p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0D12298g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553246.1">
        <property type="nucleotide sequence ID" value="XM_002553200.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DF53"/>
    <dbReference type="FunCoup" id="C5DF53">
        <property type="interactions" value="67"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DF53"/>
    <dbReference type="GeneID" id="8295486"/>
    <dbReference type="KEGG" id="lth:KLTH0D12298g"/>
    <dbReference type="eggNOG" id="KOG2798">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030612_3_0_1"/>
    <dbReference type="InParanoid" id="C5DF53"/>
    <dbReference type="OMA" id="SMWQGYY"/>
    <dbReference type="OrthoDB" id="978at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012901">
        <property type="entry name" value="CARME"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12303:SF11">
        <property type="entry name" value="AER338CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12303">
        <property type="entry name" value="CARNOSINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07942">
        <property type="entry name" value="CARME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01296">
        <property type="entry name" value="N2227"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002948706" evidence="1">
        <location>
            <begin position="25"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="431" mass="49445" checksum="CD31B2320E586D43" modified="2009-07-28" version="1">MTLSLSSFILALSVVLMLLKNNKTFKQFLANKLQKSFSKLNRHIPISSNFHLSQSKYFEKSVGLVEVYDALLQLQEYGVRAFRQNAVLFHRASSLNSIHLEQLRKLNYFEKLEGINEAVNANQKTINSIIEFTLTQLLNRNPIDNVDGRELLSACNGLGYDYQENGTLKRRVTHTKTLSPKSNQSRVNEAISHVCRDWHPSYGQERKPLVDFITARLKSSRFQGRTLVVVPGSGAGGIAHEVALAFPEFDVHSVELSTLMYLCNEFALGYDQQVSIKPFAQHFSGQLDTGNQVRDYRIDLSKVKRPSNLHVHLGDFCEFKPCEEYDQIIVASAYFIDTAENLFSYFDAIENLKSSCSELQWINVGPLKYGTQPKVQLNDKELTKLRKERKWKDLYHKCDPKDLNGYLTDEKSLYQGFYGLVKFHSTYKERK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DF71</accession>
    <name>C5DF71_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0D12716p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0D12716g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC43A transporter (TC 2.A.1.44) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553264.1">
        <property type="nucleotide sequence ID" value="XM_002553218.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DF71"/>
    <dbReference type="FunCoup" id="C5DF71">
        <property type="interactions" value="66"/>
    </dbReference>
    <dbReference type="GeneID" id="8295504"/>
    <dbReference type="KEGG" id="lth:KLTH0D12716g"/>
    <dbReference type="eggNOG" id="ENOG502QRYG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014401_1_1_1"/>
    <dbReference type="InParanoid" id="C5DF71"/>
    <dbReference type="OMA" id="CNILQQV"/>
    <dbReference type="OrthoDB" id="330047at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052599">
        <property type="entry name" value="SLC43A_AATransporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20772">
        <property type="entry name" value="PROTEIN FMP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20772:SF2">
        <property type="entry name" value="PROTEIN FMP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="100"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="161"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="194"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="310"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="349"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="378"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="404"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="428"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="470"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR22826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="522" mass="57310" checksum="D5DE317739B310D7" modified="2009-07-28" version="1">MKESSIKLIQIACAIIWCLFAAGPIFGFAALKPILVDQGVYSELCRPEGNAFQTTKVNDPSVPCTAQDLKLNFMFTVGAGTTNVAALLVGWVLDLKGPRVCGFVGSALLALGSLSLAWSHKISLLDPYITGYTLLAIGGPFVFISTFQLANSFPKRSGTILALITGAFDTSSALFFFYRLSYQNWTPDLTLKKFFTGYLIVPVFISACQLLVMPKESYKSLGAVQKLEVEGLDMEGNLPEGEDGSHIIPDENERQSLLSAHSSSIRPVLSNSRRKSVWENYVEAKLEKKTNGIFGVLHGLPAKKQIKSPWFVLMLAFTVVCMLRINYFVATVRSQEEYLLGSHKKAEKMNAIFDVALPLGGVVSIPFIGLVLDNCSTLTAMATVAVISVTIGVLGLLHSFTLNLLGILMLVAYRPFYYTVVSDYCAKVFGFDTFGTVYGLLMCISGILNMSQSLLDKTTHKTFKMNPTPVNLILVSLTVITSSSLLYFVRRESKARLERMHTLQQLGMTQESSEDSQLEYGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DF86</accession>
    <name>C5DF86_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0D13090p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0D13090g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF7 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553279.1">
        <property type="nucleotide sequence ID" value="XM_002553233.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DF86"/>
    <dbReference type="FunCoup" id="C5DF86">
        <property type="interactions" value="259"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DF86"/>
    <dbReference type="GeneID" id="8295521"/>
    <dbReference type="KEGG" id="lth:KLTH0D13090g"/>
    <dbReference type="eggNOG" id="KOG4011">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016434_2_0_1"/>
    <dbReference type="InParanoid" id="C5DF86"/>
    <dbReference type="OMA" id="KWEKMQN"/>
    <dbReference type="OrthoDB" id="153872at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08047">
        <property type="entry name" value="TAF7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037817">
        <property type="entry name" value="TAF7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006751">
        <property type="entry name" value="TAFII55_prot_cons_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12228:SF0">
        <property type="entry name" value="TATA-BOX BINDING PROTEIN ASSOCIATED FACTOR 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12228">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID 55 KD SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04658">
        <property type="entry name" value="TAFII55_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01370">
        <property type="entry name" value="TAFII55_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TAFII55 protein conserved region" evidence="7">
        <location>
            <begin position="114"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="321"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="371"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="468"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="377"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="415"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="476"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="491"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR22841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="554" mass="62547" checksum="351CBADFAD2CB355" modified="2009-07-28" version="1">MPKIKIRKPREPEPNEPEPQLKKIKIKAKGGQDANKERAGGGLKVRLSRREDSPEQQKSTPGTMKLKINLGGKDKSESTASTTPKAPRFRIKPVRVPGEGYDSEASDIEDDPLIEEGIILRVLPDVQTEFVKSCIESGDFSGLSLKWKGERHAVVKINGLQYGAVLVNLPTVIEVNKSVDRKNLLKTFDVSQMLLCVRTIEHEEDVFSLVPPDTEDLVTKHFTEYKSEIRECKKTLYRGFNGGPLTDAEASNIDQIVRKGYDYKHGLTPPLYNVRNRRFRRRLTSQEIDYVEKTVELLLSQDAETEGFSYDLVDEDILAQRSTSNEEQRQNSSDNGLDLFGDDDHEDLEMELEQALQEDRQGADQIANTNQIPAVEETGDDVEQDNGDDDDEEDEDEEGEDEADEDVVPSTAKPAVDEERQHSELLRDELEELESTLQQNRQKLEKATNPLLKSRFIESIKKLEKEVELKRKQVKSNQEPSSSHPESLAPNDEEGDEEEEEEDDEEVEDDDDDEDEEDAAAAPSNTPGRKAGANEELDQDDMDMMMLFGGEGDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFA2</accession>
    <name>C5DFA2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0D13464p</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="6">KLTH0F03872p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0D13464g</name>
        <name evidence="6" type="ordered locus">KLTH0F03872g</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
            <strain evidence="5">CBS 6340</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2009-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Genoscope - CEA"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CBS 6340</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553295.1">
        <property type="nucleotide sequence ID" value="XM_002553249.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFA2"/>
    <dbReference type="FunCoup" id="C5DFA2">
        <property type="interactions" value="1226"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFA2"/>
    <dbReference type="GeneID" id="8295537"/>
    <dbReference type="KEGG" id="lth:KLTH0D13464g"/>
    <dbReference type="KEGG" id="lth:KLTH0F03872g"/>
    <dbReference type="eggNOG" id="KOG1754">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098428_1_1_1"/>
    <dbReference type="InParanoid" id="C5DFA2"/>
    <dbReference type="OMA" id="LPAKNFG"/>
    <dbReference type="OrthoDB" id="10250260at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.30:FF:000001">
        <property type="entry name" value="40S ribosomal protein S15a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S15a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01302_A">
        <property type="entry name" value="Ribosomal_uS8_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000630">
        <property type="entry name" value="Ribosomal_uS8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047863">
        <property type="entry name" value="Ribosomal_uS8_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035987">
        <property type="entry name" value="Ribosomal_uS8_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003115">
        <property type="entry name" value="PRK04034.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11758">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S15A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00410">
        <property type="entry name" value="Ribosomal_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56047">
        <property type="entry name" value="Ribosomal protein S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00053">
        <property type="entry name" value="RIBOSOMAL_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR22857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR23941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="130" mass="14759" checksum="E404E3EF33C6CEF6" modified="2009-07-28" version="1">MARASVLADALNSINNAEKTGKRQVLIRPSSKVIIKYLQVMQKHGYIGEFEYIDDHRSGKIVVQLNGRLNKCGVISPRFNVKFNDIEKWTENLLPARQFGYIILTTSAGIMDHEEARRKHVAGKILGFVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFF3</accession>
    <name>C5DFF3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">KLTH0D14630p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0D14630g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the helicase family. SKI2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553346.1">
        <property type="nucleotide sequence ID" value="XM_002553300.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFF3"/>
    <dbReference type="FunCoup" id="C5DFF3">
        <property type="interactions" value="1001"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFF3"/>
    <dbReference type="GeneID" id="8295590"/>
    <dbReference type="KEGG" id="lth:KLTH0D14630g"/>
    <dbReference type="eggNOG" id="KOG0947">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002902_1_4_1"/>
    <dbReference type="InParanoid" id="C5DFF3"/>
    <dbReference type="OMA" id="DHVNIIM"/>
    <dbReference type="OrthoDB" id="64767at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055087">
        <property type="term" value="C:Ski complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070478">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000354">
        <property type="entry name" value="ATP-dependent RNA helicase SKI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000987">
        <property type="entry name" value="DEAD/DEAH box RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3380.30:FF:000001">
        <property type="entry name" value="Ski2 ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048392">
        <property type="entry name" value="MTR4-like_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050699">
        <property type="entry name" value="RNA-DNA_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016438">
        <property type="entry name" value="SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012961">
        <property type="entry name" value="Ski2/MTR4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048727">
        <property type="entry name" value="Ski2_beta-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040801">
        <property type="entry name" value="Ski2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12131">
        <property type="entry name" value="ATP-DEPENDENT RNA AND DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12131:SF1">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE SUPV3L1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08148">
        <property type="entry name" value="DSHCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21408">
        <property type="entry name" value="MTR4-like_stalk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21409">
        <property type="entry name" value="Ski2_beta-barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17911">
        <property type="entry name" value="Ski2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005198">
        <property type="entry name" value="Antiviral_helicase_SKI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01142">
        <property type="entry name" value="DSHCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="328"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="592"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="529"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="215"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="529"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="548"/>
            <end position="574"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR22908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1267" mass="143743" checksum="0253156B8AC117D2" modified="2009-07-28" version="1">MAMEAKNLDLEELFQSLKEITNEGSEKFENVRTKRAVDDASQEIDREDLIGKFLQPSNTLPWGLLDLFQTVPEMNFMGDPEKSKDADYDYKEFLQIPELLKRTSYHFKRKGIDGKIDSYTESVNLSEIANANASNSLSISRKINTENKSVRGSTGQLPFTPGGLTMSAFGQASDASAMASATKLLHRDEQGLFDIPQGLERGIIPQEISMDGSNQIEELNALNEIDNELENLQEISENRRKEDSKLEKDASEAILGNAAQDSDVEIDDLLPVGIDFGRTIRKAKNDLGRKEWAHVVDLNHKIENFDELVPSPARTWPFELDVFQKEAVYHLEQGDSVFVAAHTSAGKTVVAEYAIAMAKRNMTKTIYTSPIKALSNQKFRDFKEDFEDVDVGLITGDVQINPEANCLIMTTEILRSMLYRGADLIRDVEFVIFDEVHYVNDQDRGVVWEEVIIMLPQHVKFILLSATVPNTYEFANWIGRTKQKNIYVISTPKRPVPLEINIWAKDRLVPVINEKREFLEANFKAHKELVSGKSGPPDRKNEKPGASRGRGGSARGGGAGRGGAGRGGSRGAGAVGSNRSDFFKRSGPNKKTWTNLVNHLKSKDLLPAVIFVFSKKRCEDYADWLDGINFCTAKERSQIHMFVEKSVTRLKKEDRDLPQIIKMKSLVERGIAVHHGGLLPIVKELIELLFAKGLIRVLFATETFAMGLNLPTRTVVFSEIQKHDGNSLRNLNPGEFTQMAGRAGRRGLDKTGTVIVMSYTSPLEQASFKEVALGVPTRLQSQFRLTYNMILNLLRIEALRVEEMIKYSFSENSKQTLLPEHENKIKSLQSQLDSIEFYECPHCEKDLDKALDATIRYKECTKDLMNELSSGSSVFRVLNVGRLILFRDLNDCAKLGFVFRNLPKEGQVVVMTITNPSTLENGQPNHLPYLSTIPDFVKNNFKPFEKIEFFMENISMDSIEIISAFTLDVPMTDIMKQEPEVIDKFKSEVSLIIRISRKLRETKYEKKGSLKIHQAVLDREKIKTELASYECSRCPNFDVHFLPKYKEFEIQRDIKSLYHLMSDQNLSLLPDYEQRLSVLKDAGFIDQNHNVLLKGRVACEINSGYELVLTELILDNFLGDFEPEEIVALLSVFVYEGRTREEEPLVTTPRLTKGKARIQEIYKAMLNVYEKHQVPLTKDEAEFLEKKRFALMNVVYEWARGLSFKEIMEMSVESEGTIVRVITRLDEICREVKTASIIIGNSNLHMKMSQAQELIKRDIVFAASLYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFK4</accession>
    <name>C5DFK4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Coronin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0D15840g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="7">Binds to F-actin.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the WD repeat coronin family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553397.1">
        <property type="nucleotide sequence ID" value="XM_002553351.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFK4"/>
    <dbReference type="FunCoup" id="C5DFK4">
        <property type="interactions" value="380"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFK4"/>
    <dbReference type="GeneID" id="8295644"/>
    <dbReference type="KEGG" id="lth:KLTH0D15840g"/>
    <dbReference type="eggNOG" id="KOG0303">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026859_3_2_1"/>
    <dbReference type="InParanoid" id="C5DFK4"/>
    <dbReference type="OMA" id="NFQDDIY"/>
    <dbReference type="OrthoDB" id="1850764at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000197">
        <property type="entry name" value="Coronin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015505">
        <property type="entry name" value="Coronin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015048">
        <property type="entry name" value="DUF1899"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10856">
        <property type="entry name" value="CORONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10856:SF0">
        <property type="entry name" value="CORONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08953">
        <property type="entry name" value="DUF1899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16300">
        <property type="entry name" value="WD40_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM01166">
        <property type="entry name" value="DUF1899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01167">
        <property type="entry name" value="DUF1900"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 8 9" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="DUF1899" evidence="11">
        <location>
            <begin position="4"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="77"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="136"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="178"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="407"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="416"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="434"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="487"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="529"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="613"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="630"/>
            <end position="644"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00062568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU280818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR22959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="697" mass="76668" checksum="9EF05DF2849E92D3" modified="2009-07-28" version="1">MSGKFVRASKYRHVYGQVAKKERQYENIRLTNNAWDSNLIKVNPKFISVNWNSSGGGAFAVIPLNEVGKAPDQVPLFRGHTAHVLDTDFDPFNDNIIASGSDDGKIGIWEIPEDYSFHHYTNSEGEPKDLQPKKFLTGHGRKVGHVLFHPTARNVLASSSLDHTVRIWDVESGKELFTLKHPDMVTSMSFSYDGNHLATVSRDKKLRVWDIREEKIVSEGQAHTGAKNQRVVWLGNSDRLATTGFSKLSDRQIGIWDAFGLEKGDLGGFYTVDQSSGILMPFFDDSNKILYLVGKGDGNIRYYEFQNDELFELSEFQSIDPQRGFAAAPRRAVSVKDNEVLKAYKTVNDHCIEPISFYVPRKSDSFQEDIYPDAPSGTAALTADEWVSKKPVDGPVLFNMSSLYNGSEPVLKPTEKTSSPSAADKASPSVPAAKPEKDSSKEKNDKSPTKEEQTEVAEASQTQSSSVAREVGALGSSSSTLSSDANDALKEDKSVDKLLQKSSELDSVNGAEDPSKETSGWDDEDDEIETLKFEQTGKKAADKTETSVKDLKTENKCEVEKPKSEVEGSELKPETPALKPKEQEVRQQKQENGSPATLQRKPEAAETKTAAAGSQNASNQENPAEKKTAAAKATSTKAEQASSAPKSLGLKQSVEKLSDLVLELDRVVADLRQSSLDKDNRLKALEAKIEQLVKAEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFK7</accession>
    <name>C5DFK7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14 20">Inosine-5'-monophosphate dehydrogenase</fullName>
            <shortName evidence="14">IMP dehydrogenase</shortName>
            <shortName evidence="14">IMPD</shortName>
            <shortName evidence="14">IMPDH</shortName>
            <ecNumber evidence="14 20">1.1.1.205</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ordered locus">KLTH0D15906g</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 14 20">
            <text>IMP + NAD(+) + H2O = XMP + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11708"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="EC" id="1.1.1.205"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="14">Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH.</text>
    </comment>
    <comment type="pathway">
        <text evidence="14 20">Purine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Homotetramer. Seems to be able to form heterotetramers composed from more than 1 of the 3 IMPDH gene products (IMD2-4).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14 19">Belongs to the IMPDH/GMPR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.205" evidence="14 20"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553400.1">
        <property type="nucleotide sequence ID" value="XM_002553354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFK7"/>
    <dbReference type="FunCoup" id="C5DFK7">
        <property type="interactions" value="1415"/>
    </dbReference>
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    <dbReference type="GeneID" id="8295647"/>
    <dbReference type="KEGG" id="lth:KLTH0D15906g"/>
    <dbReference type="eggNOG" id="KOG2550">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022552_2_1_1"/>
    <dbReference type="InParanoid" id="C5DFK7"/>
    <dbReference type="OMA" id="MGYCGAK"/>
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    <dbReference type="UniPathway" id="UPA00601">
        <property type="reaction ID" value="UER00295"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003938">
        <property type="term" value="F:IMP dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="F:nucleotide binding"/>
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        <property type="term" value="P:GMP biosynthetic process"/>
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        <property type="term" value="P:GTP biosynthetic process"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="CBS_pair_IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00381">
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        <property type="entry name" value="Chromosome 19 SCAF14664, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005990">
        <property type="entry name" value="IMP_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015875">
        <property type="entry name" value="IMP_DH/GMP_Rdtase_CS"/>
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    <dbReference type="InterPro" id="IPR001093">
        <property type="entry name" value="IMP_DH_GMPRt"/>
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        <property type="entry name" value="IMP_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="INOSINE-5'-MONOPHOSPHATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11911">
        <property type="entry name" value="INOSINE-5-MONOPHOSPHATE DEHYDROGENASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00478">
        <property type="entry name" value="IMPDH"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01240">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51412">
        <property type="entry name" value="Inosine monophosphate dehydrogenase (IMPDH)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00487">
        <property type="entry name" value="IMP_DH_GMP_RED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 18" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 14 20" id="KW-0332">GMP biosynthesis</keyword>
    <keyword evidence="5 14 20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 14 16" id="KW-0520">NAD</keyword>
    <keyword evidence="9 14 19" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8 14 17" id="KW-0630">Potassium</keyword>
    <keyword evidence="7 14 20" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="21">
        <location>
            <begin position="120"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="21">
        <location>
            <begin position="183"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="active site" description="Thioimidate intermediate" evidence="14 15">
        <location>
            <position position="334"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14 15">
        <location>
            <position position="436"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14 16">
        <location>
            <begin position="277"/>
            <end position="279"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14 16">
        <location>
            <begin position="327"/>
            <end position="329"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="14 17">
        <location>
            <position position="329"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
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        <location>
            <position position="331"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
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        <location>
            <position position="332"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
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        <location>
            <position position="334"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
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        <location>
            <begin position="367"/>
            <end position="369"/>
        </location>
        <ligand>
            <name>IMP</name>
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        <location>
            <begin position="390"/>
            <end position="391"/>
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        <ligand>
            <name>IMP</name>
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        <location>
            <begin position="414"/>
            <end position="418"/>
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        <ligand>
            <name>IMP</name>
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        <location>
            <position position="448"/>
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        <ligand>
            <name>IMP</name>
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        <location>
            <position position="503"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
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        <source>
            <dbReference type="ARBA" id="ARBA00001958"/>
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        <source>
            <dbReference type="RuleBase" id="RU003928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="CAR22962.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="522" mass="56403" checksum="833A5AAF01961BEE" modified="2009-07-28" version="1">MSFKDCSKALEYLKSYEEKDGLSAEQLMDSNIRGGLTYNDFLILPGKIDFPSSVVNLQTKITKKITLNTPFVSSPMDTVTESEMAIQMALLGGIGIIHHNCSPEQQAAMVRKVKKFENGFINSPIVVSPTTTVAEAKAMRAKFGFCGFPVTESGSLPSKLIGIVTSRDIQFIEDDTLTIAEIMTKDLVTANVGVTLSEGNEILKNTKKGKLPIIDKDGNLVSMLSRTDLMKNQSYPLASKSATTKQLLCGASIGTIDADKDRLSLMVKAGLDVVVVDSSQGNSIFQLNMIKWIKETYPDLQIIAGNVVTREQAASLIQAGCDGLRIGMGSGSICITQEVMACGRPQGTAVYNVTKFANQFGVPCMADGGIGNIGHIVKALALGASCVMMGGMLAGTTESPGEYFFQDGKRLKTYRGMGSVDAMQKTDKKGNASTSRYFSESDKVFVAQGVSGAVVDKGSINKFIPYLYNGLQHSCQDIGVRSLTELREQVDNSTIRFEFRTASAQLEGGIQNLHSYEKRLHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFN2</accession>
    <name>C5DFN2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0D16500p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0D16500g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="CAR22987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002553379.1"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="HOGENOM" id="CLU_112567_1_1_1"/>
    <dbReference type="InParanoid" id="C5DFN2"/>
    <dbReference type="OMA" id="ISVCFVT"/>
    <dbReference type="OrthoDB" id="5138418at2759"/>
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        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33303:SF2">
        <property type="entry name" value="COA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33303">
        <property type="entry name" value="CYTOPLASMIC PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13380">
        <property type="entry name" value="CoA_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CoA-binding" evidence="1">
        <location>
            <begin position="16"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="168" mass="18549" checksum="A7A4E92A2A1C159B" modified="2009-07-28" version="1">MVKQNLNAFFGPQRLYFVCGKVYKDSSYANRLVHWFVQHKLPVVPITPNGGEVDLQADSATKDVTSTKVLPISKSISEGLQGFPHKNLIDGISVCFVTPPKITLSILKQLETEKAPIRSVWFQPGSWDMKCVKSAEDELRIEGSKIINDCILVNGDSSFTPSSLPGKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DG39</accession>
    <name>C5DG39_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein MAK16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0D02178g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the MAK16 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552819.1">
        <property type="nucleotide sequence ID" value="XM_002552773.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DG39"/>
    <dbReference type="FunCoup" id="C5DG39">
        <property type="interactions" value="1059"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DG39"/>
    <dbReference type="GeneID" id="8295039"/>
    <dbReference type="KEGG" id="lth:KLTH0D02178g"/>
    <dbReference type="eggNOG" id="KOG3064">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050888_0_1_1"/>
    <dbReference type="InParanoid" id="C5DG39"/>
    <dbReference type="OMA" id="DKGQNFC"/>
    <dbReference type="OrthoDB" id="10251342at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000460">
        <property type="term" value="P:maturation of 5.8S rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000470">
        <property type="term" value="P:maturation of LSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.110:FF:000001">
        <property type="entry name" value="Protein MAK16 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006958">
        <property type="entry name" value="Mak16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029004">
        <property type="entry name" value="Ribosomal_eL28/Mak16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23405">
        <property type="entry name" value="MAINTENANCE OF KILLER 16 MAK16 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23405:SF4">
        <property type="entry name" value="PROTEIN MAK16 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04874">
        <property type="entry name" value="Mak16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01778">
        <property type="entry name" value="Ribosomal_L28e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003352">
        <property type="entry name" value="MAK16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosomal eL28/Mak16" evidence="6">
        <location>
            <begin position="5"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="193"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="196"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="240"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="270"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR003352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR22381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="302" mass="35395" checksum="0C320C42C7973E43" modified="2009-07-28" version="1">MSDEIIWQVINQHFCAYKLKTDKGQNFCRNEYNVTGLCNRQSCPLANAKYATVKNVDGRLYLYMKTAERAHTPAKMWERIRLSKNYAKALKQVDDHLLYWNKFLIHKCKQRLTRLTQVAVTERRMELREEERHYVGIAPKVKRRDENRERKALAAAKIEKAIEKELLDRLKSGAYGDKPLNVDEKIWRKVLGHVEDEQEDDEEEYDDEEEDESDAEIEYVEDDGDEELVDVEDIERWLDDPEASDSDSFEEDEMSDSSEDEPESGFTSGKGKKGTSAARKKRPRVEIEYEEETQPAHRELAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>C5DG40</accession>
    <name>C5DG40_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Guanine nucleotide exchange factor LTE1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0D02200g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Bud</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the LTE1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552820.1">
        <property type="nucleotide sequence ID" value="XM_002552774.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DG40">
        <property type="interactions" value="258"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DG40"/>
    <dbReference type="GeneID" id="8295040"/>
    <dbReference type="KEGG" id="lth:KLTH0D02200g"/>
    <dbReference type="eggNOG" id="KOG3417">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004883_0_0_1"/>
    <dbReference type="InParanoid" id="C5DG40"/>
    <dbReference type="OMA" id="PFILMYD"/>
    <dbReference type="OrthoDB" id="10254377at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005933">
        <property type="term" value="C:cellular bud"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00155">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06224">
        <property type="entry name" value="REM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.840.10:FF:000019">
        <property type="entry name" value="Guanine nucleotide exchange factor LTE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008937">
        <property type="entry name" value="Ras-like_GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000651">
        <property type="entry name" value="Ras-like_Gua-exchang_fac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019804">
        <property type="entry name" value="Ras_G-nucl-exch_fac_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113:SF363">
        <property type="entry name" value="PROTEIN SON OF SEVENLESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00618">
        <property type="entry name" value="RasGEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00229">
        <property type="entry name" value="RasGEFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00720">
        <property type="entry name" value="RASGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50212">
        <property type="entry name" value="RASGEF_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-terminal Ras-GEF" evidence="13">
        <location>
            <begin position="38"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GEF" evidence="12">
        <location>
            <begin position="1131"/>
            <end position="1373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="602"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="636"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="881"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="943"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="602"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="964"/>
            <end position="975"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00070837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR22382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1379" mass="156204" checksum="D8CE4E59E485315A" modified="2009-07-28" version="1">MVPEDLQDEIEQGGRIFGTDDYYPMPSEEKLSFSKRGSSTLIIKTDISALIVKLTSPMDPIDYPFLSDFFLSYRKFLAPQELLKLLVSRLQWAFNESLSADSNKQQIGQIASVRTFVLIRHWILNYFAQDFLTDDALRSQFVNSVNSLSWGASDVAPKSICGIVINIKKSWGFCLRLMWEGLSFEDQPTSANEWLEFKIKDVATHLSTKNGKRLSFYALQSSQNPEFRNRSVLSLYVGKENFQLPEHNARVNTKQGAKIKQRTASMFLFPQDNLSSAVLKVSNDKVGNSDEVSQNAPNSSGVSEVIKDLQYPPSPSIDKVIPPTPAKKVEFILRSSYFPSEILSEEEANTSSTSQKLKKEPHLHSYNKGMMGLLSKWKKNHTLRSANQKINQESPSAEFENLIKLVFSITSLENKQYSSEELKSVVSSKFDILSARTIDEVEYFLAVENDLLSKIRKQDHFTTVEGGADEQTQERDAETVSAMDNLNLYKTVSSIASSVISLSKTLQFQHAVSPSAAARERRRVRSTDGLLGNKSVLNFTDALLTSDVNTRILENDAPRKLVFHGHSTTRERHTNSELKFRYSNMADAVIRLNHSTPSVMQTSALTKSTNGTRSRGSPTKGVRISSSITEIDDTFSKHNESTPQAEEVSNAGSHISRPLKRKESLDNLREFNFEENFDQKNGSQSKLDHKINHNELQSDADDDNSSFFTTLDDMSMSRNSKHMTPASSAAQKSVTSSGRISIIRSRGPSVSNAVTISTTNLVAKDGAFLKLDEELLNNQEEIRNLEERTSALFFDKVEEEGAKNIRSSIATSVSTQLLFSSKQSSPNKIEKNDLLDNDEFPQGNKLIRLSSIPSMHSIVSGDSFSSFHTINSFEQNSRADISHKLTVDTPSTGAKPEKNAPNDSLLVPDAEGSSGNKYLFSPDTDSLDFASPEKNMEDLKNKFISPITSETTSFNEDDLSRDNEGEEGEPTEEQQLDNGYQEKPHNEHHRRSITPIVSPYKANDSRLNRFAKLTDESLHGDPVNVALMKLEGTFSKTDQNKEQSDSDMERLKERRLTGLGARERNSFLIERRRQVKSEVPFTPCTKDFSYERDQKVTNDQITELLQNYKIQDSRLNISNAEQHVPFILMYDSKSIAEQLTLIEREILNEIDWKDLLDLRVKRKVLPVTSWLQLLLQNEQLSGIDLAIARFNLTVDWIISEIVLTRDNKLRRNTIQRFIHVAEHCKLFQNYNTLMQIVLALSSLVVQSYRDAWRLVEPGDLLSWESLKNVPSLEKNYYNIRMLLNDIEPIKGCIPFIVVYLSDLTLNSEKSNWITPHQVLNYSKFQTNVQIVKNFIQRAQWSKFYNISVNDELLSKCVYISCLTHEEIDHLKSQQTKSAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGM7</accession>
    <name>C5DGM7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0D06578p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0D06578g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553007.1">
        <property type="nucleotide sequence ID" value="XM_002552961.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGM7"/>
    <dbReference type="FunCoup" id="C5DGM7">
        <property type="interactions" value="519"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGM7"/>
    <dbReference type="GeneID" id="8295238"/>
    <dbReference type="KEGG" id="lth:KLTH0D06578g"/>
    <dbReference type="eggNOG" id="KOG1649">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011216_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGM7"/>
    <dbReference type="OMA" id="EQVHMYH"/>
    <dbReference type="OrthoDB" id="515064at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000228">
        <property type="term" value="C:nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006939">
        <property type="entry name" value="SNF5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10019">
        <property type="entry name" value="SNF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04855">
        <property type="entry name" value="SNF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="65"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="194"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="257"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="664"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="65"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="103"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="194"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="268"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="672"/>
            <end position="682"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR22569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="783" mass="89637" checksum="C3ECCCE33877D117" modified="2009-07-28" version="1">MNNLQTNPFANIGTTSFSLSQVPQHILQNLTPVQLQMIQQKHQQLLMERQALMQQQQQQQQQQQQQQQQQQQQPQHHQNQQHPSMSQRHQPGELPQHQRQHQHQQQQQQHHLHQQQQQHQLKQQQQQQQLQQQQLQQQQQQQQQQQQQQQQQQQQQQQQQQLQQQQITANAGQSTALPQQLNLAQRAQILKAKQQQLDTNQQQAASTPTGVPALNLPPQIAQLPPHVQQRVLNNLKQQAAAKNNPAAVAAITAAQQQLAGRMSNHQSPTPPSAQFPQQPIPQTPGLPTPPVPQASVPLPKFQTIFQDPPERQMSSHSHWSDKIERGELDIKEPDSNLLIYEQTLKRDRENGEQLIKERTGYEPFSKYGFSHKEYLTRLLQDLNYIKEVKAARMKSITATTQGLVSKSIWGDGYSGYGNGFSNTVTSVSTGQTGPDGRQSTHYNVGEIYQQAMYESHEELVPVRLEFDAERDNFSLRDVFVWNKNEKIIKIEELVTEMLRDYRFARNDQFFETVVQSIKEQINEFQPDPFLENEGDRFGGDDLRIKIQLDIVVGQNQLMDAFEWDISNPSNSPEEFAETLSQELELPGEFSTAIAHSIREQVHMYHKSLSLTCYQFDGSTVEDEDIRSRLLPLVTLNEVFRNSMNTKVFTPSLLKVSHAELERLDRDKDRDTRRKRRQGRFNRRGVGPGTAVTLNDGSLPDLSDVPKTFRIPVPSTILPGGVDLGPSVNSYRTESSSEYMARPPEPALEQPNPCCRIIDHNPGQYLLVRIKLPSKTDGAISQGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGW3</accession>
    <name>C5DGW3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Superoxide dismutase 1 copper chaperone</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0D08778g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29036"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CCS1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553093.1">
        <property type="nucleotide sequence ID" value="XM_002553047.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGW3"/>
    <dbReference type="FunCoup" id="C5DGW3">
        <property type="interactions" value="308"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGW3"/>
    <dbReference type="GeneID" id="8295329"/>
    <dbReference type="KEGG" id="lth:KLTH0D08778g"/>
    <dbReference type="eggNOG" id="KOG4656">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056632_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGW3"/>
    <dbReference type="OMA" id="KNVWEER"/>
    <dbReference type="OrthoDB" id="666972at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006801">
        <property type="term" value="P:superoxide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.200">
        <property type="entry name" value="Superoxide dismutase, copper/zinc binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036423">
        <property type="entry name" value="SOD-like_Cu/Zn_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49329">
        <property type="entry name" value="Cu,Zn superoxide dismutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMA" evidence="5">
        <location>
            <begin position="9"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR22655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="244" mass="26159" checksum="02F9D2C14C2D46F3" modified="2009-07-28" version="1">MTSPTSEDSFEATYAVDMHCNGCVEDIKSCLAGIPNLSLDFHVPKKLLAVKGTAAPSVIISTLQKCGRDAIIRGTGKPNSAAVSILETFEPVADSDTPVRGLARIVGVSDTKTWVDITLNGLPQPGKYYASVRECGDISKGAQSTGGPVYKFDEPIDCSSPSDLGKNLYSGQAFLSAPLRVWELVGRSFMVTSDPQHRAGHGYEICGVLARSAGVWENDKQVCACSGKSVWQERKDALEHNIQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DH26</accession>
    <name>C5DH26_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Glycerol-3-phosphate dehydrogenase</fullName>
            <ecNumber evidence="11">1.1.5.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0E00836g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>a quinone + sn-glycerol 3-phosphate = dihydroxyacetone phosphate + a quinol</text>
            <dbReference type="Rhea" id="RHEA:18977"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.1.5.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Polyol metabolism; glycerol degradation via glycerol kinase pathway; glycerone phosphate from sn-glycerol 3-phosphate (anaerobic route): step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.5.3" evidence="11"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553524.1">
        <property type="nucleotide sequence ID" value="XM_002553478.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DH26"/>
    <dbReference type="FunCoup" id="C5DH26">
        <property type="interactions" value="691"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DH26"/>
    <dbReference type="GeneID" id="8291662"/>
    <dbReference type="KEGG" id="lth:KLTH0E00836g"/>
    <dbReference type="eggNOG" id="KOG0042">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015740_4_1_1"/>
    <dbReference type="InParanoid" id="C5DH26"/>
    <dbReference type="OMA" id="PHIVKPM"/>
    <dbReference type="OrthoDB" id="264015at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004368">
        <property type="term" value="F:glycerol-3-phosphate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006072">
        <property type="term" value="P:glycerol-3-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.870:FF:000005">
        <property type="entry name" value="Glycerol-3-phosphate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.9.10:FF:000037">
        <property type="entry name" value="Glycerol-3-phosphate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.870">
        <property type="entry name" value="Alpha-glycerophosphate oxidase, cap domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031656">
        <property type="entry name" value="DAO_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038299">
        <property type="entry name" value="DAO_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000447">
        <property type="entry name" value="G3P_DH_FAD-dep"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11985">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11985:SF15">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16901">
        <property type="entry name" value="DAO_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01001">
        <property type="entry name" value="FADG3PDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00977">
        <property type="entry name" value="FAD_G3PDH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00978">
        <property type="entry name" value="FAD_G3PDH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0274">FAD</keyword>
    <keyword evidence="5 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="12">
        <location>
            <begin position="66"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-glycerophosphate oxidase C-terminal" evidence="13">
        <location>
            <begin position="485"/>
            <end position="624"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF16901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR23087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="641" mass="71093" checksum="925DDF4FD0672F71" modified="2009-07-28" version="1">MFARTVSRRVLAGATLAAGGYAAFALQRDRSAHNEVHVSIPLKAEAPAIPSRSELLDKMAKTDQFDVLVIGGGATGTGCAVDGATRGLNVALVEMNDFASGTSSKSTKMAHGGVRYLEKAFWELSKAQLDLVIEALNERGHMLNTAPHLCKVLPIMIPVYNYWQVPYFYVGCKMYDLFAGSQNLKSSYMMTARRASEVAPMLDASILKAGLVYHDGSFNDSRMNAALAVTAIERGATVLNYMEVTRLLKNEKTGRVEGAMARDRETGKEFRINAKVVVNSTGPFSDRILQMDNAKDGLPRNDLVQFANEGHDSIGSRVAVSNPRMVVPSAGVHIVLPAFYCPKQIGLLDAKTSDGRVMFFLPWQGKVLAGTTDIPMKQVPENPTATEADIQDILKELQHYIKFPVKREDVLSAWAGIRPLVRDPRTLKEGDDVSSTQGLVRNHFLFTSDNGLVTIAGGKWTTYREMAEETIDEVVKQGHFQAKPCITKKIKLAGAEGWDPNFVAMLAQEYHLSSKMAEHLANNYGTRSPIICEMFRRDEKNQLPVTFGGRENVTVYKNVNFDSFRYPFTIAELKYSVKYEYTRTALDFLMRRTRFGFLDAKQALEAVKGTVSIMGDELGWDENKRLSEIQQASDFIKTFGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHD4</accession>
    <name>C5DHD4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0E03454p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0E03454g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553632.1">
        <property type="nucleotide sequence ID" value="XM_002553586.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHD4"/>
    <dbReference type="FunCoup" id="C5DHD4">
        <property type="interactions" value="185"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHD4"/>
    <dbReference type="GeneID" id="8291780"/>
    <dbReference type="KEGG" id="lth:KLTH0E03454g"/>
    <dbReference type="eggNOG" id="ENOG502S9SB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_137480_1_1_1"/>
    <dbReference type="InParanoid" id="C5DHD4"/>
    <dbReference type="OMA" id="HYKGKDD"/>
    <dbReference type="OrthoDB" id="2567806at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1250.10">
        <property type="entry name" value="Ribosome maturation protein SBDS, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036786">
        <property type="entry name" value="Ribosome_mat_SBDS_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019783">
        <property type="entry name" value="SDO1/SBDS_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01172">
        <property type="entry name" value="SBDS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89895">
        <property type="entry name" value="FYSH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosome maturation protein SDO1/SBDS N-terminal" evidence="2">
        <location>
            <begin position="3"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR23195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="108" mass="11919" checksum="75C49EE900960B5F" modified="2009-07-28" version="1">MAATKYFYKGEETDLVIFVESQELADQYLKDPTIGKLTEAVGVFKIFTNQQGEGAEGELGEASKAQIENEFGKGIKIEEAIARILQEGSPNARGDVNKDKFQSRNDSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHI1</accession>
    <name>C5DHI1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">KLTH0E04510g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553679.1">
        <property type="nucleotide sequence ID" value="XM_002553633.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHI1"/>
    <dbReference type="FunCoup" id="C5DHI1">
        <property type="interactions" value="585"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHI1"/>
    <dbReference type="GeneID" id="8291828"/>
    <dbReference type="KEGG" id="lth:KLTH0E04510g"/>
    <dbReference type="eggNOG" id="KOG1609">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006729_0_0_1"/>
    <dbReference type="InParanoid" id="C5DHI1"/>
    <dbReference type="OMA" id="ALYFQYD"/>
    <dbReference type="OrthoDB" id="1108038at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16702">
        <property type="entry name" value="RING_CH-C4HC3_MARCH6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13145:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MARCHF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13145">
        <property type="entry name" value="SSM4 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="183"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="415"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="441"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="482"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="522"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="556"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="619"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="678"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="906"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1057"/>
            <end position="1076"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1102"/>
            <end position="1120"/>
        </location>
    </feature>
    <feature type="domain" description="RING-CH-type" evidence="15">
        <location>
            <begin position="14"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="267"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="368"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="277"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="302"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="315"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAR23242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1257" mass="143530" checksum="2B4D45362B2DEE79" modified="2009-07-28" version="1">MGVAAEESSGENEHSAALNASCRICRGENTSDSPLFHPCKCRGSIKYIHESCLLEWVASKNVDLARPGANIKCDICHYSIQFKTLYDENMPDRIPVGLVIRTTLGSVFKEAQAVIRIVMATTLLVVGIPLAWNTLGKLIAMLLFGGSLPIQGDFWKSLLYGFEATVPENPSKLNIALQLLKNYRFSLTQIVFLTVIHIALYFQYDMVVREPIFREMVYHNIGPRFNGEELATSLHNAMQFIRANEERLRAQRAVPLQEDLLRGQDLGFGNEGIQAGNDEHNDDRHNSTHRNRGSEDGIFYNGEDDEDDDDYEPNEAVASEESIASSSSSDHEIDDEERQQLDENNGVFNFTMNHGRDNDLEFMVQNAQQNAEREQHEEPAVGPGRNDGAADPEEAWNQERPAIFGVQLRFRNLPFYFLAATAFLAIYLYLTYAIPTFLGNLLLTIYSKVFVWAFRGFIKLVEISRTPTLYKELSRRFPRISSLNLWLNTKAMQIFSFISYVYTNYVDYNNKTSTVAQSIPALATYSTLLGILCASTGIMSRGYGPKNGMKNSNLRFVFHLFFAIRCSLKVFLLFGIELVGFPVLAGLMIDFSLISPCLTSHNKFLLIGGLDIWSPAKWVLYWVIGTFYMCWFAKYVAMIRKYIIRPGVLFFIRSSDDPNIRILHDSLIHPMRVQISRLLLSIMLYALFIVIGFGFHTRILFPLILKSKLLPFETAQDELFFYGIAAILHQPMRITEANKSFNLYVRQYWTKAFDLSCKKLRLSSFILNKDISTERGYIVYRNLFYRFFFSEKAKWSNPDLYVDPKTPAQADELFKANLEIHAYFVPDGTLMRVPANDLISRNYVQTLFVPVTKENKLLKPLDVESIKERNKENAGDYSYLDDQSTEFDAYTVVYTPPNFRSRFSTLLLLIWVFASVLCMTLALFFNFVGRLMLVAVLSPFSGNEKARGYLLAYRKFSSVSLWPIAIGAWITMRGTELFHKFKMTRVVHDEYAVHPEAGDGAENDGGVREPENLEPAADFEENEEGANAPLPQDRLQWQGRLLFILLGKMQAIKYCIVLYYNFAFVSLFVEIFILRIEKPWKIDTFFTPNSWAEFGTVVSERLVTFGLFFTWFVLASIIDARKSIQRMLQLGQDERLKSLKILVIDLVKECVLTAGLSFALQMALCTYEYLLKGPPSYESVLSAYSFLRTVRPSLSSSEVPWTIPHKLFFVIPPVLSGTFYLPKIFKSVSDFIKKSVVNTREQVYGRGKTLTNLPEGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHJ4</accession>
    <name>C5DHJ4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">assimilatory sulfite reductase (NADPH)</fullName>
            <ecNumber evidence="4">1.8.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">KLTH0E04796g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">This enzyme catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>hydrogen sulfide + 3 NADP(+) + 3 H2O = sulfite + 3 NADPH + 4 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13801"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17359"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.8.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sulfur metabolism; hydrogen sulfide biosynthesis; hydrogen sulfide from sulfite (NADPH route): step 1/1.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.2" evidence="4"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553692.1">
        <property type="nucleotide sequence ID" value="XM_002553646.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHJ4"/>
    <dbReference type="FunCoup" id="C5DHJ4">
        <property type="interactions" value="494"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHJ4"/>
    <dbReference type="GeneID" id="8291841"/>
    <dbReference type="KEGG" id="lth:KLTH0E04796g"/>
    <dbReference type="eggNOG" id="KOG1158">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003662_1_0_1"/>
    <dbReference type="InParanoid" id="C5DHJ4"/>
    <dbReference type="OMA" id="DYDRYIF"/>
    <dbReference type="OrthoDB" id="1856718at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004783">
        <property type="term" value="F:sulfite reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd06207">
        <property type="entry name" value="CyPoR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.990.10:FF:000010">
        <property type="entry name" value="Sulfite reductase [NADPH] flavoprotein component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.80:FF:000011">
        <property type="entry name" value="Sulfite reductase flavoprotein component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.990.10">
        <property type="entry name" value="NADPH-cytochrome p450 Reductase, Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.920.10">
        <property type="entry name" value="Pyruvate-ferredoxin oxidoreductase, PFOR, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003097">
        <property type="entry name" value="CysJ-like_FAD-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023173">
        <property type="entry name" value="NADPH_Cyt_P450_Rdtase_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002869">
        <property type="entry name" value="Pyrv_flavodox_OxRed_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384">
        <property type="entry name" value="NITRIC OXIDE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384:SF109">
        <property type="entry name" value="SULFITE REDUCTASE [NADPH] FLAVOPROTEIN COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00667">
        <property type="entry name" value="FAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53323">
        <property type="entry name" value="Pyruvate-ferredoxin oxidoreductase, PFOR, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0288">FMN</keyword>
    <keyword evidence="9" id="KW-0521">NADP</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FAD-binding FR-type" evidence="15">
        <location>
            <begin position="649"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="583"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="611"/>
            <end position="620"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00052219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00059320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAR23255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1036" mass="114706" checksum="9A34A79F97DE514B" modified="2009-07-28" version="1">MGLEFSTNPFGIPSNWKESNAYTTPVTAISSVLAGNLESVFSYKTFSEPDLLDGALKKWSSKGYTTLQFEELQLRSGAGLAPLGFARGSGHSSLYGIITPGYGLSYFANAFTREAETKNRFLFNVGALEYNEESGQVASDFVTPVKIAASMGLPVVSPTSVKEVKHTALLAIALAKFGKPIGAVNLFDGANYAKTVLNIDEQESQDIIGELSSSLVVGSTFDQILDKFNEVSSAKLHNFDYHGDENAETVFVTFGCVESELFSTSLRGNTNNVGHIAIRIPLPFDTEKFVAQIPKSAKNLVVIGQSLDGQSPTTLKSQISAALFFQKAKTVKVSEYLYLPSFVWSADAVKQVVSTFAPEFSSYVSHQAENFIYWASDSSVNLDVASRLVHALSLVDNREVSFRTKFDNNANGGVFQAQFSSGKQSGSHVFSNIDSANVAVVENLSILNAFDVAKTVDNNGVVLIITEKSIKDKDLSDSKVLAEELKIPISFLRQAVDKNLLLAFLDTETIGDKEETKGRTASFVTQAAFWKYAYKLSVAESVRRIWSSAGSDIELLAAVLSETITTAFEVGLHEVPAEKLSKSIPEVSPEKDEEGPLPSLLTETCFKPNPRKSEEHSEVELSSKASVAQQLSFKEAFATESSLRPDLPMKNFIVKVKENRRVTPDDYDRYIFHIEFDITGTGLTYGIGEALGVHARNNQPLVREFLESYGLSENDIIQVPSKEDHDVLESKTVLKAFTENLDLFGKPPKAFYQSLIDFASNEDEKRKLEELVSPEGAVLLKKYQDVEYYTYADIFKLFPSARPGITDLVRIISPLKRREYSIASSQRVHPNEVHLLIVVVDWIDNKGRKRFGQSSKYLAELPVGAELVVSVKPSVMKLPPSPQQPVIMSGLGTGLAPFKAIVEEKFWQKQQGHDIGKVFLFLGSRHKREEYLYGELWEAYKDAGIVTHIGAAFSRDQPHKIYIQDRIREALKELQTALIDEKGSFYLCGPTWPVPDITKALQDIIKADADERGEKVNLDEAIEELKETSRYILEVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHZ5</accession>
    <name>C5DHZ5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0E08448p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0E08448g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytidine and deoxycytidylate deaminase family. ADAT3 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553843.1">
        <property type="nucleotide sequence ID" value="XM_002553797.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHZ5"/>
    <dbReference type="FunCoup" id="C5DHZ5">
        <property type="interactions" value="44"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHZ5"/>
    <dbReference type="GeneID" id="8292004"/>
    <dbReference type="KEGG" id="lth:KLTH0E08448g"/>
    <dbReference type="eggNOG" id="KOG2771">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013817_2_0_1"/>
    <dbReference type="InParanoid" id="C5DHZ5"/>
    <dbReference type="OMA" id="YWPLVWR"/>
    <dbReference type="OrthoDB" id="3180714at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052717">
        <property type="term" value="F:tRNA-specific adenosine-34 deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01285">
        <property type="entry name" value="nucleoside_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11079">
        <property type="entry name" value="CYTOSINE DEAMINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11079:SF156">
        <property type="entry name" value="INACTIVE TRNA-SPECIFIC ADENOSINE DEAMINASE-LIKE PROTEIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00383">
        <property type="entry name" value="dCMP_cyt_deam_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="3">
        <location>
            <begin position="167"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR23406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="323" mass="37281" checksum="C64603F1F968857C" modified="2009-07-28" version="1">MVKKKQNPLRINYQEGIIEEKLWQIRDRANINEPKLVNVWTVDIKIGESRSFVDFIRQSVQKMEPVTFQHIKRIKKCDENNECLTVIVCSHFMIKEKDDFMHFMQEAPFSFQNVKGSSVVPQNGPPTKSLSMEWSNLYWPLVWRGNPNDQILNSYVFDMNFIEATLKQISELSLAEQASGKQAAVVSAFVNPKNNEVIFVSDHRHVCSPLDHSIMRGIRSVAELEHQKKQDHTNKGESETYLCLDFDVYTTHEPCSMCSMALIHSRIKRCIYLTPMNKTGCLESESGDGYCMHNNHSLNSKYEAFRWIGDEYPAAQIESNVCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>C5DI01</accession>
    <name>C5DI01_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Pre-mRNA-splicing factor CWC24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">KLTH0E08580g</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
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        <text evidence="1 14">Involved in pre-mRNA splicing.</text>
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    <comment type="subunit">
        <text evidence="14">Associated with the spliceosome.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Nucleus</location>
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    <comment type="similarity">
        <text evidence="3 14">Belongs to the CWC24 family.</text>
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        <property type="protein sequence ID" value="CAR23412.1"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ZF_RING_2"/>
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    </dbReference>
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        <location>
            <begin position="131"/>
            <end position="159"/>
        </location>
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        <location>
            <begin position="190"/>
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        <location>
            <begin position="131"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
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            <begin position="28"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="70"/>
            <end position="81"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00009161"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00020647"/>
        </source>
    </evidence>
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    </evidence>
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        <source>
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        <source>
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            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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            <dbReference type="RuleBase" id="RU367110"/>
        </source>
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            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAR23412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
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    </evidence>
    <sequence length="250" mass="28161" checksum="76A3D1058F9C58D1" modified="2009-07-28" version="1">MFKKRAIKCDQASSKRQKFTEDDVTETPVCSKNTRTTESLAYQAGPSSLQNKITNNDENPKKDTLKRSRSISPPQETSIPSSKPEDFGQTEASHEHRAMSPIEPASKSVGAISALGPDKKHPNLRTTLYMDYQPDICKDYKQTGYCGYGDSCKFLHARDDFKSGWKLNQDWKLEPTEEESKELEKIPFKCLICKGDYKNPVMTKCKHYFCNSCFIARTKKTTKCAVCNDDTYGVAKTAKGLQQILKNQGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-04-02" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>C5DI21</accession>
    <name>C5DI21_LACTC</name>
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        <submittedName>
            <fullName evidence="2">KLTH0E09064p</fullName>
        </submittedName>
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        <name evidence="2" type="ordered locus">KLTH0E09064g</name>
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        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
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        <name type="synonym">Kluyveromyces thermotolerans</name>
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        <lineage>
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            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
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                <person name="Talla E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
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    </reference>
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        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="1">
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            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="159" mass="15536" checksum="1A3DBAA7C2D7BAFB" modified="2009-07-28" version="1">MAKPYIRARFAHLLKSYLEVSNIIDHRSCVSTHHFSHQTPKMASSSTSAVSTNTNLVSAAAVAASSVSASASSGASSAKSSASSAASSVKSSVTSGASSVSSSASKSASSSKSSSSSSASSSSSSGSNAITAVANPLSWKYGAVIGATMLGSFILGSGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>C5DI39</accession>
    <name>C5DI39_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0E09482p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0E09482g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
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        <name type="synonym">Kluyveromyces thermotolerans</name>
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        <lineage>
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            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
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                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002553841.1"/>
    </dbReference>
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    <dbReference type="FunCoup" id="C5DI39">
        <property type="interactions" value="69"/>
    </dbReference>
    <dbReference type="GeneID" id="8292051"/>
    <dbReference type="KEGG" id="lth:KLTH0E09482g"/>
    <dbReference type="eggNOG" id="ENOG502QRC6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101860_0_0_1"/>
    <dbReference type="InParanoid" id="C5DI39"/>
    <dbReference type="OMA" id="FEDRDKF"/>
    <dbReference type="OrthoDB" id="4041975at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="243" mass="28656" checksum="1CE3B524181A49D4" modified="2009-07-28" version="1">MWNRVVSHLSNLEDVAINYLRRFPCMAEYTYDPISARITLFLLVVGTMAFINELYITIEMTFLQKETYAELNKAPLNVEDLRNHRMVMDDEFHGREWWDEKSGIVIEEFESRERFFAKPVHVAHVYVECNIIDGETGSDLLSRPFQFHIEFSPEEYENEKRPEFGCKLIVLRRKLYHFFKETELYQELVAHNSKKDESVRFTVSSGVKIYNAAKELLPTTIDDIQLCFLKIETGDSIRCDFVV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-04-05" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIF1</accession>
    <name>EXO5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName>Exonuclease V, mitochondrial</fullName>
            <shortName>Exo V</shortName>
            <ecNumber>3.1.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Defects in morphology protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">EXO5</name>
        <name type="synonym">DEM1</name>
        <name type="ordered locus">KLTH0E12012g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Single strand DNA specific 5'exonuclease involved in mitochondrial DNA replication and recombination. Releases dinucleotides as main products of catalysis. Has the capacity to slide across 5'double-stranded DNA or 5'RNA sequences and resumes cutting two nucleotides downstream of the double-stranded-to-single-stranded junction or RNA-to-DNA junction, respectively.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the EXO5 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553999.1">
        <property type="nucleotide sequence ID" value="XM_002553953.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIF1"/>
    <dbReference type="FunCoup" id="C5DIF1">
        <property type="interactions" value="28"/>
    </dbReference>
    <dbReference type="GeneID" id="8292166"/>
    <dbReference type="KEGG" id="lth:KLTH0E12012g"/>
    <dbReference type="eggNOG" id="ENOG502QR0P">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019985_0_0_1"/>
    <dbReference type="InParanoid" id="C5DIF1"/>
    <dbReference type="OMA" id="LQVMYYR"/>
    <dbReference type="OrthoDB" id="354769at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045145">
        <property type="term" value="F:single-stranded DNA 5'-3' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019190">
        <property type="entry name" value="EXOV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14464">
        <property type="entry name" value="EXONUCLEASE V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14464:SF4">
        <property type="entry name" value="EXONUCLEASE V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09810">
        <property type="entry name" value="Exo5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0269">Exonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0496">Mitochondrion</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0809">Transit peptide</keyword>
    <feature type="transit peptide" description="Mitochondrion" evidence="2">
        <location>
            <begin position="1"/>
            <end status="unknown"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000406688" description="Exonuclease V, mitochondrial">
        <location>
            <begin status="unknown"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="511"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="514"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="520"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="552" mass="63638" checksum="A9D5A4B4B376ACD8" modified="2009-07-28" version="1" precursor="true">MTLKRFSLFARNIHLPKKTNAASVESNGSLAPTDEELEVINSLPPFQHALQLSSQSATKAKRSYLETKLPKVQKLFKSSRDPAYLAYDFPQGLSNPYVDAHARPVTNPVTGDTKYRGTPRLSVTKLLTKRWCELRETYDIYSRIPIFEHSQVKQGRKEHQRLEEEVHAPPANIEEFEANFEVDIPDDPFHSLVEDWYLSIVRLVTLFQKGQAREILCHGYLSSRTSGFVRGKVKDDNDVLVSGIIDHLILRQRRAKNNVPSSLTQVLSESVDINLAQFLNLLPSLVQQASKDFEIVVSDVKTRSTKTIPRQQTVVKASKMQVMYYRFLLEDLGRDAETTYQKLLINAQRRGFNVDQPINPAKIINLLEIDPMIRGDMERLQRGDQLGFKPFDSEAELNTSQPVYTLEEFDEKITDLRTRQKYETLFTTWNTPVTLRYFAARLALMYENVGPLLSQNLMIEYYCGDVNFHNISFSFDAEELERQSFDSAQFWFGKRAIEPIKPNMKNLQTYCKYCDYESVCLWRKEGRQACKTLGQELVRLYNGNSPNAITRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIK1</accession>
    <name>C5DIK1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0E13156p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0E13156g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the MEF2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554049.1">
        <property type="nucleotide sequence ID" value="XM_002554003.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIK1"/>
    <dbReference type="FunCoup" id="C5DIK1">
        <property type="interactions" value="733"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIK1"/>
    <dbReference type="GeneID" id="8292218"/>
    <dbReference type="KEGG" id="lth:KLTH0E13156g"/>
    <dbReference type="eggNOG" id="KOG0014">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022725_0_0_1"/>
    <dbReference type="InParanoid" id="C5DIK1"/>
    <dbReference type="OMA" id="VQRQFHN"/>
    <dbReference type="OrthoDB" id="1898716at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008301">
        <property type="term" value="F:DNA binding, bending"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033554">
        <property type="term" value="P:cellular response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00265">
        <property type="entry name" value="MADS_MEF2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1810.10:FF:000013">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050142">
        <property type="entry name" value="MADS-box/MEF2_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033896">
        <property type="entry name" value="MEF2-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48019">
        <property type="entry name" value="SERUM RESPONSE FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00404">
        <property type="entry name" value="MADSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00350">
        <property type="entry name" value="MADS_BOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="8">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="100"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="249"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="518"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="571"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="105"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="144"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="158"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="174"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="188"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="249"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="282"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="330"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="364"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="571"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="583"/>
            <end position="599"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR23612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="629" mass="66673" checksum="69C2F8992AF289CB" modified="2009-07-28" version="1">MGRRKIAIEPILDERNRTVTFIKRKAGLFKKAHELAVLCQADVAVIILGANNTFYEFSSVDANDLIRHYQNDELPHDVKGPANYGNYTKKDRVVIHGKNRKRGGAAHAAAARASRSASHSIEGSRPGTGAHGVAPDSSSGGGTAAATALGSKRGAGSDSHDGHDDHDHDNDHDRDDDDDDEDDDDENSNSNNGADASDAQASGPRAPHQQQFPKRPKTAAAASNPGFNPLQQQVQRQFHNLYAVASGSERTSNFSTQPPSSFSRSTLPNIYPHGPGGVAAATASNPSPGRNFAQTQSFRSNSIPLPFDPSPRDSMQVPSSTAAPVHIDRSYSISHSRSSTNTTATNRPALRVQIPSSTGVAIKSEPSSASSPSHPASTSQFTRRNDGHIELPHPGVSKVTTPVSASGAAPNIGFDRLDKTHGVLPSASSIGGAGAGAGPGFLFNGLPSALNASPSIQQYFATPLQHSSAGGNGLMATSNGVSHPHGYIMHKQIHMAQQQKQQQQQQQQQQQQQQQQQQQQQQQQQADSASGPMSGLLPSKFANDMMMPSPNMSMSMFQDWGFQRAAAQNNAAVGTGGQPPTDSNASSSAAPQAQNNGSSGLTPYINVNQTPLTNKYFVFEEPNSEKNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIK9</accession>
    <name>C5DIK9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase</fullName>
            <ecNumber evidence="4">3.5.1.52</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Peptide:N-glycanase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0E13332g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of an N(4)-(acetyl-beta-D-glucosaminyl)asparagine residue in which the glucosamine residue may be further glycosylated, to yield a (substituted) N-acetyl-beta-D-glucosaminylamine and a peptide containing an aspartate residue.</text>
            <dbReference type="EC" id="3.5.1.52"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the transglutaminase-like superfamily. PNGase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.52" evidence="4"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554057.1">
        <property type="nucleotide sequence ID" value="XM_002554011.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIK9"/>
    <dbReference type="FunCoup" id="C5DIK9">
        <property type="interactions" value="119"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIK9"/>
    <dbReference type="GeneID" id="8292226"/>
    <dbReference type="KEGG" id="lth:KLTH0E13332g"/>
    <dbReference type="eggNOG" id="KOG0909">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031058_0_1_1"/>
    <dbReference type="InParanoid" id="C5DIK9"/>
    <dbReference type="OMA" id="AWDKPRL"/>
    <dbReference type="OrthoDB" id="409136at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000224">
        <property type="term" value="F:peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006516">
        <property type="term" value="P:glycoprotein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.620.30:FF:000004">
        <property type="entry name" value="Peptidase (PNG1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.620.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050883">
        <property type="entry name" value="PNGase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002931">
        <property type="entry name" value="Transglutaminase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12143">
        <property type="entry name" value="PEPTIDE N-GLYCANASE PNGASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12143:SF19">
        <property type="entry name" value="PEPTIDE-N(4)-(N-ACETYL-BETA-GLUCOSAMINYL)ASPARAGINE AMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01841">
        <property type="entry name" value="Transglut_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00460">
        <property type="entry name" value="TGc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Transglutaminase-like" evidence="10">
        <location>
            <begin position="196"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="335"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="344"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR23620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="373" mass="43918" checksum="D445642D55BA839E" modified="2009-07-28" version="1">MACLAEYNKVNKVIKLNHQIQEYLNTKTMDRQLDFRQIAARFLDLYKQKVVRTTRNISDSQRFQSLIRSNEFARNIFSLSQNLCSRYENDIWQARVLDCIDLETIYSNVEAMQGSEDDYLDRLVKELLRYFKRDFFKWCDKPECSNCCDSEHQTVVGHQGPNAEERRFDCGVVETYKCQSCGSITRFPRYNDPVKLLETRKGRCGEWCNLFMLFLKAFGIESRYVWNREDHVWCEFYSTHLKRWVHVDSCEQSFDEPHIYSNNWGKHMSYAIAFGRGGCSEVSKRYIVKNQLPRDQISEDDLQFLLSFITKRLRMNLSDSEIYKLSCRDEQERLQLMKGDSTDRQATGSSSVPQGRQSGSASWKSTRGEDGKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIL6</accession>
    <name>C5DIL6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">3-methyl-2-oxobutanoate hydroxymethyltransferase</fullName>
            <ecNumber evidence="3 6">2.1.2.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0E13486g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is transferred onto alpha-ketoisovalerate to form ketopantoate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = 2-dehydropantoate + (6S)-5,6,7,8-tetrahydrofolate</text>
            <dbReference type="Rhea" id="RHEA:11824"/>
            <dbReference type="ChEBI" id="CHEBI:11561"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="EC" id="2.1.2.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the PanB family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.11" evidence="3 6"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554064.1">
        <property type="nucleotide sequence ID" value="XM_002554018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIL6"/>
    <dbReference type="FunCoup" id="C5DIL6">
        <property type="interactions" value="173"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIL6"/>
    <dbReference type="GeneID" id="8292233"/>
    <dbReference type="KEGG" id="lth:KLTH0E13486g"/>
    <dbReference type="eggNOG" id="KOG2949">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036645_0_1_1"/>
    <dbReference type="InParanoid" id="C5DIL6"/>
    <dbReference type="OMA" id="VLVWTDM"/>
    <dbReference type="OrthoDB" id="425211at2759"/>
    <dbReference type="UniPathway" id="UPA00028">
        <property type="reaction ID" value="UER00003"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003864">
        <property type="term" value="F:3-methyl-2-oxobutanoate hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015940">
        <property type="term" value="P:pantothenate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd06557">
        <property type="entry name" value="KPHMT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.60:FF:000003">
        <property type="entry name" value="3-methyl-2-oxobutanoate hydroxymethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00156">
        <property type="entry name" value="PanB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003700">
        <property type="entry name" value="Pantoate_hydroxy_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00222">
        <property type="entry name" value="panB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001452">
        <property type="entry name" value="PRK00311.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20881">
        <property type="entry name" value="3-METHYL-2-OXOBUTANOATE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20881:SF0">
        <property type="entry name" value="3-METHYL-2-OXOBUTANOATE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02548">
        <property type="entry name" value="Pantoate_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000388">
        <property type="entry name" value="Pantoate_hydroxy_MeTrfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0566">Pantothenate biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR23627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="312" mass="34014" checksum="8D8C30B70D9F3D41" modified="2009-07-28" version="1">MIRNAVHHLHRSYSSYLSPVVRPKTLADLFSKHVSREPITMITAYDYISGSWAQAANCDMILVGDSLAMCALGYESTTEISLEEFCFHVQAVSRSPGDAFIVVDMPFGSFESSIEKGVETAISLMKCSPKVGALKLEVGSHSLSTHNPDYSLKLAAELCSRGIPVMGHIGLTPQRVHSLGGYKVQGSRSAQDAAAIFETARQLKKLGCFSILVECVPHKVSQHITETLDIPTIGIGAGSGTSGQVLVQSDILGMLPGRVPKLAQKYTDLHAQSVDALKHYVADVTLHKFPDNDKNGFKIKEDVWREFLEIVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIU0</accession>
    <name>C5DIU0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0E15158p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0E15158g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554138.1">
        <property type="nucleotide sequence ID" value="XM_002554092.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIU0"/>
    <dbReference type="FunCoup" id="C5DIU0">
        <property type="interactions" value="658"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIU0"/>
    <dbReference type="GeneID" id="8292309"/>
    <dbReference type="KEGG" id="lth:KLTH0E15158g"/>
    <dbReference type="eggNOG" id="KOG0273">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007609_1_1_1"/>
    <dbReference type="InParanoid" id="C5DIU0"/>
    <dbReference type="OMA" id="KWNKCGN"/>
    <dbReference type="OrthoDB" id="1367865at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034967">
        <property type="term" value="C:Set3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.960.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024977">
        <property type="entry name" value="Apc4-like_WD40_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045183">
        <property type="entry name" value="Ebi-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22846:SF2">
        <property type="entry name" value="F-BOX-LIKE_WD REPEAT-CONTAINING PROTEIN EBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22846">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12894">
        <property type="entry name" value="ANAPC4_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08513">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Anaphase-promoting complex subunit 4-like WD40" evidence="6">
        <location>
            <begin position="201"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="201"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="242"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="330"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR23701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="509" mass="55663" checksum="0D12470FC0C07CF9" modified="2009-07-28" version="1">MSLTSEELNYLIWRYLQESGKEVSALALQEESRVLEFESKFGERVPIGTLVNFVQKGILYAESELMVRYDRESMSLDPGLNARDFNLAQALEVDKERVPELKAVQRFALEDQVNAGTHSPGIDSSDKIGGEDSSKFIKTLHESLRLPQSTVCQWNPKNTSILSWGGPESTATIATFEISDGKPRVLQETKCQHPLGISASTGKLTNDITCLEWSPSGESIVTGVENGELRLWTSDGKLQNVFNFHKSGITTIKWNEDSTHFLTCDVENVAIVWNSLTGTALQHISLKESGATESLGVDASWVGPEKFVIPGLQGAIALCGIGESRPLGKLNGHTKTITALDYNYETKMLLSGSDDTTLRVWRSGALSSSNCFMGNTLGITSAFWLDQDRVISTSFDGSVRVWSQSTNALLGLSMVDGIPIFCGSLSPDKRIFAIGKMDGEVCLYHIGKLLELLNDKSINDSTHPTPIPICGDYQHNIEGSYVNGIAWNASSTHLSISYSSSDTELIFVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIW6</accession>
    <name>C5DIW6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0E15730p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0E15730g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554164.1">
        <property type="nucleotide sequence ID" value="XM_002554118.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIW6"/>
    <dbReference type="FunCoup" id="C5DIW6">
        <property type="interactions" value="69"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIW6"/>
    <dbReference type="GeneID" id="8292335"/>
    <dbReference type="KEGG" id="lth:KLTH0E15730g"/>
    <dbReference type="eggNOG" id="ENOG502R1X7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031057_0_0_1"/>
    <dbReference type="InParanoid" id="C5DIW6"/>
    <dbReference type="OMA" id="GWLNICL"/>
    <dbReference type="OrthoDB" id="2357318at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055334">
        <property type="entry name" value="PEX8-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39214">
        <property type="entry name" value="MICROBODY (PEROXISOME) BIOGENESIS PROTEIN PEROXIN 8 (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39214:SF1">
        <property type="entry name" value="MICROBODY (PEROXISOME) BIOGENESIS PROTEIN PEROXIN 8 (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR23727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="574" mass="65283" checksum="CC9A95746FA4548E" modified="2009-07-28" version="1">MALLARQSQADQAVDYIINALSKPNFHGDIKAILNNLVYYVPRVRSKVNLDRLLHAMFCSKAWETALQNDLISLQESSEAIFSWKLEISEPVISVNEFYQAWDSAVTDCNMWSAGQIAIIGGALKAQNKFKSLQTRYFLDERGSVVEIYTRWKRQYFLPLWRQIFMRSVRTPKRLEQLALILSAVFSPGDSAFVPCDPLCEVLIGLSIAYIQDPSRGEPSVARNISNIAKTLEAALQYASKKVTSRSLKLICTATFELSLRELNNPKSVYSSQAYSNQLLTVVLIFRGCVTQPVIPDIWYLQIIVSLYYMNFILQDFGRVGFESYEFIYEVCTTAIKMKPESYVNSLEVMRGNLWPSVMNNAVNDSRALFLLTFVESTVADMTIDAKLLHSILVPTLNHFVRSPNTVICESAHAAQLALFSNHVSPDCLQKWKCGNCLEYLDLSTNQFLAGFLSSSQVVHVYTSIVREAAFLHPYNDDLVREILHFTYLRILNSWKMGPEIVTTLIECLVVQIPHASQKYIIDWLDNCLSLINSCGQGKEKLLDHLWLQLTSNEITDDAFSWWYENVVRASSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJ73</accession>
    <name>C5DJ73_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0F14058p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0F14058g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554799.1">
        <property type="nucleotide sequence ID" value="XM_002554753.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJ73"/>
    <dbReference type="STRING" id="559295.C5DJ73"/>
    <dbReference type="GeneID" id="8293025"/>
    <dbReference type="KEGG" id="lth:KLTH0F14058g"/>
    <dbReference type="eggNOG" id="KOG0234">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_756645_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJ73"/>
    <dbReference type="OMA" id="CRLFNAG"/>
    <dbReference type="OrthoDB" id="267323at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003873">
        <property type="term" value="F:6-phosphofructo-2-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004331">
        <property type="term" value="F:fructose-2,6-bisphosphate 2-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006003">
        <property type="term" value="P:fructose 2,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006000">
        <property type="term" value="P:fructose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003094">
        <property type="entry name" value="6Pfruct_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013079">
        <property type="entry name" value="6Phosfructo_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606:SF44">
        <property type="entry name" value="6-PHOSPHOFRUCTO 2-KINASE_FRUCTOSE 2,6-BISPHOSPHATASE LONG FORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606">
        <property type="entry name" value="6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01591">
        <property type="entry name" value="6PF2K"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00991">
        <property type="entry name" value="6PFRUCTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="6-phosphofructo-2-kinase" evidence="4">
        <location>
            <begin position="3"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="6-phosphofructo-2-kinase" evidence="4">
        <location>
            <begin position="104"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="317"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR24362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="366" mass="40254" checksum="9D213AD997B3070B" modified="2009-07-28" version="1">MTEKAVIVMVGLPARGKSYIAQRIVSYYRSAYGDGGGCRLFNAGKERRFLAADAQDAPPHPALYRTVTHQPHQPRHPGDSAEIDDDLSGAEAEVGTGPAPPQTLFDTQNDDAVRARDQIALATVQKMCVWLQSDNAHRVAIFDATNTTAARRAMVVDTIRLHTSGQTPVIFIESICTDAEVIHQNIVHKVRHSPDYLEDPDKHWCYLDFKTRLENYERIYEPCDLAAEFPQQEFSESLALLRVVNQGAKIQLQGQASPAHESTLMCLLHGIYHNTLDAATFLPLALDTITKARSACSSTSSSLFSLSSLMLSSNATEEERVRESVDEAAASDPKPPNRALEQELELILSGAASRAEQGSLRRDAND</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJU7</accession>
    <name>C5DJU7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Mediator of RNA polymerase II transcription subunit 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0F19250g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Mediator complex subunit 7 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555023.1">
        <property type="nucleotide sequence ID" value="XM_002554977.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJU7"/>
    <dbReference type="FunCoup" id="C5DJU7">
        <property type="interactions" value="822"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJU7"/>
    <dbReference type="GeneID" id="8293259"/>
    <dbReference type="KEGG" id="lth:KLTH0F19250g"/>
    <dbReference type="eggNOG" id="KOG0570">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065214_0_1_1"/>
    <dbReference type="InParanoid" id="C5DJU7"/>
    <dbReference type="OMA" id="IHDSYSM"/>
    <dbReference type="OrthoDB" id="10253553at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037212">
        <property type="entry name" value="Med7/Med21-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009244">
        <property type="entry name" value="Mediatior_Med7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044888">
        <property type="entry name" value="Mediatior_Med7_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21428">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21428:SF11">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05983">
        <property type="entry name" value="Med7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140718">
        <property type="entry name" value="Mediator hinge subcomplex-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0010">Activator</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="192"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="192"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR24586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="220" mass="24817" checksum="52A3179CC37747A5" modified="2009-07-28" version="1">MSVGGANEIAALYPPPPPYIKYFTDENLTKLEAQRKSGEASEADEDLEFLIPPPVPAEGHYRAFGSVWHVKDELPDLSSMGMTQLYQGSADGTASSYQSKIQELHKLLRSLLLNFLELTGILSINPERFAEKVEHIQTILVNFHHLLNEYRPHQSRESLIMLLEAQLEHKRSEIQHIEQVCSDVRAKLRRLVDGDGPKNEDAPSEPEPQSHVQKEAESLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJZ8</accession>
    <name>C5DJZ8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0F00638p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0F00638g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554236.1">
        <property type="nucleotide sequence ID" value="XM_002554190.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJZ8"/>
    <dbReference type="GeneID" id="8292427"/>
    <dbReference type="KEGG" id="lth:KLTH0F00638g"/>
    <dbReference type="eggNOG" id="KOG0475">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003181_2_0_1"/>
    <dbReference type="InParanoid" id="C5DJZ8"/>
    <dbReference type="OMA" id="NSHIDND"/>
    <dbReference type="OrthoDB" id="44789at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005247">
        <property type="term" value="F:voltage-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03684">
        <property type="entry name" value="ClC_3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3080.10:FF:000030">
        <property type="entry name" value="Chloride channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3080.10">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014743">
        <property type="entry name" value="Cl-channel_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001807">
        <property type="entry name" value="ClC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45711">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45711:SF3">
        <property type="entry name" value="CLC CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00654">
        <property type="entry name" value="Voltage_CLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00762">
        <property type="entry name" value="CLCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81340">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0868">Chloride</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="196"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="267"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="385"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="463"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="502"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="561"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="587"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="637"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="668"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="89"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="31"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="96"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR23799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="911" mass="101114" checksum="D8A75E3CF44C2588" modified="2009-07-28" version="1">MPFKNAAAHNDSNTFHSAMKGSSRRIRGRSSQRGGVSRAASVNLRLEGLPGPGVINEVVQGTESPHPHSKALSQLTQISSIRSPIRNAGPQDYFHINSQGSSPTKYGSTDQGRNGQESEASLYSESTQPPYLFPKFRNLTETLNSETLLPRNYYDDFTSVDWVKDYLYDVWQKQQMSALRGFTGKLKRVYGNAQDWVLITVVAFSCSLLAFFIDKAEELLVDLKRGYCKSNFFFNQQQCCASYTCANWKLWPEALRFLHGGVLRSDFMVYTFLSLILALSAAKVTLTTKYINPLAFQTDKKIFKTMYHAYGSGVPEVKTILSGFIIRKFLGTYTLFSKSIALVLAIASGLSLGKEGPYVHLATCVGNIVSRQFAKFKLNGIERRVILSASAAAGVTLAFGSPLGGVLFSLEEVSYFLPGNQLFKTFFSAIMANLFLRLLNPYGTGKAVLFEVKYSSDWQTTELFLVTIIGAIGGVFGAYFCKFASFWGSWFRSKSFIREKHLREVALIALLTAALTFPNSYTNISVAELLANLASPCFSPDDFTGKHGLCPVDRKSFPSEIVPLSYALIVKIVLTSITFGVKVPAGIYVPSMVIGALFGRIFATWFEHLASLYPNFFVFTQICNKSGLEGVCIDMGIYAMISAGAFMAGVTRMNVTLAIIIFELTSSYNYVVPISIAIAVSNLVAHVIEPQSLYEMLMRKNDLPFLDNRKVRDFRNHDGLREIVSTLKQGNGNIYVNLSEQKAFSSAVLREHLSNLQKEGLVDGCIPIINNGKLAGVLPAPELELALDKLKCFADEFKLTQDLKVRLLEVDTSIPSRGGDKNFTSPIESFPNLTSQEEWLAVNEILEKLCDLTPIVEKFPIMLDLNSSPSLVELIFTKLGNRFISVLDGDEFVGLVHRKHFIDYCRDKAIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DK93</accession>
    <name>C5DK93_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0F02750p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0F02750g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554331.1">
        <property type="nucleotide sequence ID" value="XM_002554285.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DK93"/>
    <dbReference type="FunCoup" id="C5DK93">
        <property type="interactions" value="53"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DK93"/>
    <dbReference type="GeneID" id="8292523"/>
    <dbReference type="KEGG" id="lth:KLTH0F02750g"/>
    <dbReference type="eggNOG" id="ENOG502QR7Q">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_357169_0_0_1"/>
    <dbReference type="InParanoid" id="C5DK93"/>
    <dbReference type="OMA" id="NHLKDIM"/>
    <dbReference type="OrthoDB" id="534815at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990423">
        <property type="term" value="C:RZZ complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1440">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2230">
        <property type="entry name" value="Retrograde transport protein Dsl1, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021876">
        <property type="entry name" value="Dsl1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021875">
        <property type="entry name" value="Dsl1_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038442">
        <property type="entry name" value="Dsl1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046362">
        <property type="entry name" value="Zw10/DSL1_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12205">
        <property type="entry name" value="CENTROMERE/KINETOCHORE PROTEIN ZW10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12205:SF0">
        <property type="entry name" value="CENTROMERE_KINETOCHORE PROTEIN ZW10 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11989">
        <property type="entry name" value="Dsl1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11988">
        <property type="entry name" value="Dsl1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Retrograde transport protein Dsl1 N-terminal" evidence="2">
        <location>
            <begin position="23"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="Retrograde transport protein Dsl1 C-terminal" evidence="3">
        <location>
            <begin position="550"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="392"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF11989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR23894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="740" mass="84219" checksum="FFF0DD60BAE120D4" modified="2009-07-28" version="1">MAQACGIIDQELHCISQQLKRILNDQDAILAGIESDPNLKMEGSARREEPSDVQAILNQDVQLSKKLGALKELNLVANLIDEFKANFELWELENCYYSLQNLHKRLEKVDMSLFPIQFQGLLKVHIDSLHVDLLSRIDKVMRQFWDISARSVTFNQKIAVDRDDVLLEYDSFSLFLKSCFFSQDHFEPTSWFVATMNMGDAKDKVTNLLATAFNEWVRLQTVQEKLKQFLFSANVCLSLKGCTLGASENPGNLDDTLKSFEALVMFMKDVTTAEDTAVILAKLGNTLLTEIVKFVKQNSSHLFAPGTNYKNSVVSLNTMLTELSHKGGKSWQYAGRELDNLINDDSVINNLMLDKLLQEQISAIRKQFASGDWKNKRAIKVKARKTSLSVLSGERSKAHKTGTGTSDDEWAWDHDNVSAEVGEDEDGWGSETEIALDSKEAHDPIVLSKKDAEGENDWQNAWDEAASLGDDFDDTMKVTQVPDLFKQIFTDFEAGCEKIGQNKISAVVDYKRNVLLTSFMAMCMCRYEEWWQLYHDVDFILSESADARKLYRLHELNSHFVNTHVENLKKTARKIMGQQLQNFQASDKSPNWGITIESLLPYIETAALPALLKLRNPRILSGFLEYLYADCLVNEILSWRMISEKNSENLAELINLLLTGTDVRQVETDPKIVHSREKLEIIGRVLTAHLTDIMDMFSNGDFYLFSTDEVVQWIVMLFADTGLRRECIDEIRTIREEQGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKC8</accession>
    <name>C5DKC8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Aconitate hydratase, mitochondrial</fullName>
            <shortName evidence="9">Aconitase</shortName>
            <ecNumber evidence="9">4.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0F03586g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="9">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the aconitase/IPM isomerase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.-" evidence="9"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554366.1">
        <property type="nucleotide sequence ID" value="XM_002554320.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKC8"/>
    <dbReference type="FunCoup" id="C5DKC8">
        <property type="interactions" value="293"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKC8"/>
    <dbReference type="GeneID" id="8292559"/>
    <dbReference type="KEGG" id="lth:KLTH0F03586g"/>
    <dbReference type="eggNOG" id="KOG0453">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006714_2_2_1"/>
    <dbReference type="InParanoid" id="C5DKC8"/>
    <dbReference type="OMA" id="KWPETFG"/>
    <dbReference type="OrthoDB" id="2224430at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003994">
        <property type="term" value="F:aconitate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.19.10:FF:000002">
        <property type="entry name" value="Aconitate hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.499.10:FF:000003">
        <property type="entry name" value="Aconitate hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.499.10:FF:000004">
        <property type="entry name" value="Aconitate hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1060.10:FF:000001">
        <property type="entry name" value="Aconitate hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1060.10">
        <property type="entry name" value="Aconitase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="entry name" value="Aconitase, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="entry name" value="Aconitase, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050926">
        <property type="entry name" value="Aconitase/IPM_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015932">
        <property type="entry name" value="Aconitase_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006248">
        <property type="entry name" value="Aconitase_mito-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01340">
        <property type="entry name" value="aconitase_mito"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005558">
        <property type="entry name" value="PRK07229.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43160">
        <property type="entry name" value="ACONITATE HYDRATASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43160:SF2">
        <property type="entry name" value="HOMOCITRATE DEHYDRATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="Aconitase iron-sulfur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52016">
        <property type="entry name" value="LeuD/IlvD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01244">
        <property type="entry name" value="ACONITASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5 9" id="KW-0408">Iron</keyword>
    <keyword evidence="6 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha" evidence="10">
        <location>
            <begin position="58"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="domain" description="Aconitase A/isopropylmalate dehydratase small subunit swivel" evidence="11">
        <location>
            <begin position="585"/>
            <end position="713"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR23929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="787" mass="85675" checksum="E1761982A58B4998" modified="2009-07-28" version="1">MLSARSVSRRHLATQVAANFANVPKEFQSRVPPYAKLIANLEKVKAITNNSPLTLAEKILYSHLCDPEESIKSSDLRDIRGLEYLKLHPDRVAMQDASAQMALLQFMTTGLAQTAVPASIHCDHLIVGRDGESQDLSSSIATNKEVFDFLQSCADKYGIQFWGPGSGIIHQIVLENFSAPGLMMLGTDSHTPNAGGLGAIAIGVGGADAVDALTGTPWELKAPKILGVKLTGSMQGWTSPKDVITKLAGILTVRGGTGYIVEYFGDGVKTLSCTGMATICNMGAEIGATTSTFPYQEAHSRYLKATGRSVMADAADVALNQYNFLKADSGAEYDKVIEINLSELEPHVNGPFTPDLSTPISEFGAKCTKENWPQKISAGLIGSCTNSSYQDMARAADLVRQASQAGLKPRIPFFVTPGSEQIRATLEKDGLVKTFEDNGAIVLANACGPCIGQWDRQDVMKTSKETNSIFTSFNRNFRARNDGNRNTMNFLTSPEMVTAMIYSGDAQFNPMRDTIKLDDGSDFKFKAPSGDELPQTGFVSGRKEFYPPTDPTPDASVEIKVSPTSDRLQLLEPFKPWDGKELKTNVLMKVEGKCTTDHISAAGVWLKYKGHLENISNNTLIGAQNKETGEVNKAYDFDGTAYGIPELMIKWKQEERPWTVVAEHNYGEGSAREHAALSPKFLGGQILLVKSFARIHETNLKKQGMLPLTFANEEDYDRISSGDVLETVGLVEMIEKEGANGGLLDVKITKPSGESFVIKTKHTMSKDQVEFFKAGSAINYIGEVRRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKE5</accession>
    <name>C5DKE5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0F04026p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0F04026g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554383.1">
        <property type="nucleotide sequence ID" value="XM_002554337.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKE5"/>
    <dbReference type="FunCoup" id="C5DKE5">
        <property type="interactions" value="280"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKE5"/>
    <dbReference type="GeneID" id="8292578"/>
    <dbReference type="KEGG" id="lth:KLTH0F04026g"/>
    <dbReference type="eggNOG" id="KOG1502">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_9_2_1"/>
    <dbReference type="InParanoid" id="C5DKE5"/>
    <dbReference type="OMA" id="KNEECWA"/>
    <dbReference type="OrthoDB" id="2735536at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05227">
        <property type="entry name" value="AR_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000191">
        <property type="entry name" value="Methylglyoxal reductase (NADPH-dependent)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF564">
        <property type="entry name" value="STEROL-4-ALPHA-CARBOXYLATE 3-DEHYDROGENASE, DECARBOXYLATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="3">
        <location>
            <begin position="3"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR23946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="341" mass="37880" checksum="4CE333D3ADF647E7" modified="2009-07-28" version="1">MPVLVTGCTGFIAQYIVRDLLEQNYEVIGTVRSKEKGDTIERLFNNDPSLTLEVVEDIAAPDAFDNVFEKYGSKIKVILHTASPFHFNATDYENEMLKPALHGTRSVLESIKKYGSKTVERVVVTSSIAAIKDMEKVANADFVYTEESWNPITWEESKLNSQNAYRGSKKFAEKAAWDFWEENKNIVKFELTTVNPSMIFGPQLFDENAKGVLNTSAEVINSFLKSGPDADISALKNDFVDVRDVSKAHLLAFQKTDTVGKRLGLSSGQFNGQDLARILNERFPQLQGTIPQVSGDGANKTEPYAKFDNSNTKEILGFPFRGLEETLSDTVAQILKAQGGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKF3</accession>
    <name>C5DKF3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0F04202p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0F04202g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 34 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554391.1">
        <property type="nucleotide sequence ID" value="XM_002554345.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKF3"/>
    <dbReference type="FunCoup" id="C5DKF3">
        <property type="interactions" value="117"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKF3"/>
    <dbReference type="GeneID" id="8292586"/>
    <dbReference type="KEGG" id="lth:KLTH0F04202g"/>
    <dbReference type="eggNOG" id="KOG4748">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021434_1_0_1"/>
    <dbReference type="InParanoid" id="C5DKF3"/>
    <dbReference type="OMA" id="IKTYNHF"/>
    <dbReference type="OrthoDB" id="205108at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000136">
        <property type="term" value="C:mannan polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000009">
        <property type="term" value="F:alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008630">
        <property type="entry name" value="Glyco_trans_34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31306">
        <property type="entry name" value="ALPHA-1,6-MANNOSYLTRANSFERASE MNN11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31306:SF10">
        <property type="entry name" value="ALPHA-1,6-MANNOSYLTRANSFERASE MNN11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05637">
        <property type="entry name" value="Glyco_transf_34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR23954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="414" mass="47537" checksum="551C93DAB4165552" modified="2009-07-28" version="1">MALKPKLKPMSTSNSLQKRSMQFKNVFVDSKKRRKQAFTLSVFVGIALVLYLVSGLFGRGRPTVNKYPPVHGHYINEIATTKPLIFPAVENAAQLKEMNMRSLHILRIDMDGNKKYVLKETDQPITEKERKQMTDPHEIIKRDYLDHGKLVFRKGTDSPEVVIVTLVDFDNYDPQTLIKVVQNRVNYAQRHRYGVYVRWAQEFIPLLEKQNLKESYEYLKPMIMRAAFHAFPRAKYFWFLDQDALIMRMDLSLQNHLLDPKILQLAILKNVPVVKDSNIKTYNHFLPGNAEIIFPQTNGGYLDSSSFIAASGMYSKAMFDYLSDPLVRDFPWQNFAHCVSHALQWHPSILAKTALIVPKTIASVYDPAKKADEKPGVDAFHYTAGDLVASFYGCKDRGSCIKDIDTFYDKVQTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKL7</accession>
    <name>C5DKL7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0F05742p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0F05742g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the type 2 lipid phosphate phosphatase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554455.1">
        <property type="nucleotide sequence ID" value="XM_002554409.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKL7"/>
    <dbReference type="FunCoup" id="C5DKL7">
        <property type="interactions" value="404"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKL7"/>
    <dbReference type="GeneID" id="8292653"/>
    <dbReference type="KEGG" id="lth:KLTH0F05742g"/>
    <dbReference type="eggNOG" id="KOG2822">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019266_1_1_1"/>
    <dbReference type="InParanoid" id="C5DKL7"/>
    <dbReference type="OMA" id="GRWEYPY"/>
    <dbReference type="OrthoDB" id="301434at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042392">
        <property type="term" value="F:sphingosine-1-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03388">
        <property type="entry name" value="PAP2_SPPase1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969:SF28">
        <property type="entry name" value="DIHYDROSPHINGOSINE 1-PHOSPHATE PHOSPHATASE LCB3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969">
        <property type="entry name" value="SPHINGOSINE-1-PHOSPHATE PHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="155"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="183"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="213"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="247"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="309"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="384"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="9">
        <location>
            <begin position="111"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR24018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="407" mass="46212" checksum="981E4CCD65EE69E3" modified="2009-07-28" version="1">MTRQRALSVSSEASLDSKIEVTTYKEKHAFADPGNHSRDHFKRRMSPVRFAMRDWLLQFTKHQSEGLYKLQCKFRCPALDKYFAYTSLMGSHMFYVIMVPMPRWLGFSVMCRDLVYILGYSIYLSGFLKDYWCLPRPKSPPLTRVALSPYTTKEYGAPSSHTANATGVTLLLLWYVYRLENASISWKLAAIAGALFYYFTLTLGRIYCGMHGLLDIVSGALIGALCFFVRWAAVTYTHIDTANIQEWGWWFPVASVSLGLVLLFNHAKPVDHCPCFEDSVAFVGVISGLESSDWLLWHVGRSADLHIPYSWSEFGVLLTLRRIAVGVLMVLIWKSVVGKTLIYKLLNLVWEDDRGRYKLEHIDNPSEEVPLFTGRSRTELLGRFLIYAGVAGTVALASPPLFRLLGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKM4</accession>
    <name>C5DKM4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Potassium transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0F05896g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the TrkH potassium transport family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554462.1">
        <property type="nucleotide sequence ID" value="XM_002554416.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKM4"/>
    <dbReference type="FunCoup" id="C5DKM4">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKM4"/>
    <dbReference type="GeneID" id="8292660"/>
    <dbReference type="KEGG" id="lth:KLTH0F05896g"/>
    <dbReference type="eggNOG" id="KOG1341">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005947_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKM4"/>
    <dbReference type="OMA" id="RMTNDGI"/>
    <dbReference type="OrthoDB" id="9999863at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140107">
        <property type="term" value="F:high-affinity potassium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003445">
        <property type="entry name" value="Cat_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004773">
        <property type="entry name" value="K/Na_transp_Trk1/HKT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015958">
        <property type="entry name" value="Trk1_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051143">
        <property type="entry name" value="TrkH_K-transport"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00934">
        <property type="entry name" value="2a38euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31064:SF30">
        <property type="entry name" value="HIGH-AFFINITY POTASSIUM TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31064">
        <property type="entry name" value="POTASSIUM TRANSPORT PROTEIN DDB_G0292412-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02386">
        <property type="entry name" value="TrkH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002450">
        <property type="entry name" value="K+_transpter_TRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0630">Potassium</keyword>
    <keyword evidence="4 10" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="50"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="107"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="635"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="674"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="703"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="766"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="831"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="906"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="934"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="197"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="287"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1007"/>
            <end position="1146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="323"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="338"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="359"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="397"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="427"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="458"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1007"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1057"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1073"/>
            <end position="1090"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1100"/>
            <end position="1119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1121"/>
            <end position="1130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR002450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR24025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1146" mass="129555" checksum="75229E1A326F4487" modified="2009-07-28" version="1">MQAWKTLSRRPTLASINITYHKTIGHRLRDIIEYVGNVTHPFVKHIFPNFIAVHYFYIITLSIIGSILIYPIKNFAYIDILFMATGAATQGGLNTINVNDMTLYQQIVLYLICCLATPIWIHGVLAFVRLYWFERYFDGIRDWSKKNFKVRRTKTLIQREMTRTATEAQRRHKTAGRANNDFQSKLFSGQMIKREEQGGVKENYEMNSLKDAPTGGSEHKDPHTSSSTGTTKSDQEDSPAIKFGDMPKPHKSPKKPADIEMFSGRRRSEDITPADMFRSIAMLQNRHNEEEEEHDGPALVIRGPAERRQGSDPSWASVPHSGNIEETKDKDSRKPATHSINSPSSAARSPSFHKEADYSPTQWSSQNESRPRTPSSIQFNIGMVPSSKPGNPQYHSSNRKKLNTRIMKKLTPRNRLRKRLRKTASNTDEDGNDADFEQDSLTSGTDHDQDEESLASFGLSSQHSSQAPNMEKVQSTLAVPSGDATGGSKFYKRSLTMEAPGNSDWDVLTKSPSFQKMIYNKWKDERRRKKGVIHSAYRRPSLAHSTSELDSGFNLGHDARTMTDEALFGNNHDEEYFGTTFEEPTDRPRLKRMSTNYLSWQPKIGRNSTFVGMNEIQRAELGGVEYRAIKLLCKLLLLYYIGFHVMGMVMLLPWITQMKHYAKLVREDGVAPAWWGFFTAMSSFNDLGLTLTPDSMVSFNKAIYPLIVMMWFIIIGNTGFPVFLRFIIWVMFKLSPELSLIKESLGFLLDHPRRCFTLLFPSAPTWWLLFILVTLNAIDLILFIVLDLNSQVVEGLTSGFKVLNGLFQAVSTRTAGFTILNLSKLHPSIQVSYMLMMYVSVLPLAISIRRTNVYEEQSLGIYDNGGDDAHHKDSSSDSQSEDERNLEKSAKSFIGAHLRRQLSFDLWFLFLGLFIICISEGGKIQDTNKPDFTVFQVLFEIVSAYGTVGLSLGYPNTDQSFSGQFNTFSKVIIIGMLIRGRHRGLPYSLDRAIILPSQSLERRDQLQDLKQEQHTTEAKDPIVDYLRRQSRSAKGQLRSLLSRRKRPTSDESVPLRSFSNHENLRQPSASNVSNLSPAPRSSPRSQSLSQGDAETYGGEESSSISSDFQDMAASGSSIGPRNEEVPHFSDEPPTALEGPRAFEPNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKN9</accession>
    <name>C5DKN9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0F06248p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0F06248g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554477.1">
        <property type="nucleotide sequence ID" value="XM_002554431.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKN9"/>
    <dbReference type="FunCoup" id="C5DKN9">
        <property type="interactions" value="213"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKN9"/>
    <dbReference type="GeneID" id="8292676"/>
    <dbReference type="KEGG" id="lth:KLTH0F06248g"/>
    <dbReference type="eggNOG" id="ENOG502QSU5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076919_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKN9"/>
    <dbReference type="OMA" id="ELWGVQF"/>
    <dbReference type="OrthoDB" id="4082764at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024297">
        <property type="entry name" value="Pho86"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11124">
        <property type="entry name" value="Pho86"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="301" mass="33563" checksum="74593A5426ABB303" modified="2009-07-28" version="1">MAKKTQPQMVEQMDASLHEPINKDAPLTIYGSKLKPELATAALNLSIDFLKQQQSLANQHVMWHRKTLGTAFLCAFVYLAPRATFPKGSKSVMGTVVQFVLMNKAQLVYFAVVTILAASFIFTFLARFTEDLFKRKISQVVDTNGVAVFGVDLTELVKGDIKDKSKLDNTQVIVYRETPIALASVVENSLMSSNDALVMGVSTIGSRRVYLQSGIIEDLLDWALIRTKNIQKTGKFEAGNSMKLLVEVYSFDKVIKKTLKSKGFSLIKAVKLEESRLLGGLFGVKKELWGVQFHFDSKKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKZ7</accession>
    <name>C5DKZ7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0F08778p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0F08778g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SEC10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554585.1">
        <property type="nucleotide sequence ID" value="XM_002554539.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKZ7"/>
    <dbReference type="FunCoup" id="C5DKZ7">
        <property type="interactions" value="839"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKZ7"/>
    <dbReference type="GeneID" id="8292791"/>
    <dbReference type="KEGG" id="lth:KLTH0F08778g"/>
    <dbReference type="eggNOG" id="KOG3745">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008002_1_0_1"/>
    <dbReference type="InParanoid" id="C5DKZ7"/>
    <dbReference type="OMA" id="PLCKHHY"/>
    <dbReference type="OrthoDB" id="125856at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009976">
        <property type="entry name" value="Sec10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048627">
        <property type="entry name" value="Sec10_HB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048625">
        <property type="entry name" value="Sec10_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12100:SF0">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12100">
        <property type="entry name" value="SEC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07393">
        <property type="entry name" value="Sec10_HB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20667">
        <property type="entry name" value="Sec10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex component Sec10 N-terminal" evidence="6">
        <location>
            <begin position="54"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="Exocyst complex component Sec10-like alpha-helical bundle" evidence="5">
        <location>
            <begin position="178"/>
            <end position="806"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF20667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR24148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="816" mass="92509" checksum="5D2E08193EF12BAC" modified="2009-07-28" version="1">MNSLYELDPKFRELLTLDNFLGGLTVHEFVHKISNENSLKDRAAHAGSWENVDPKPYIRTFESTVKELKRLSADADSRKGQLEQEVAKYELLHSQQVLQLSGNLRSIVKDTGDLDSKLTDVTQVVSPLGEKLEKAIKRKNMYIKSVELISHYSSFYSQGSSENLEKLRVSENWNPRARAAILVKNLLALARKVETKSLPKTLTTTAAIEKYSENMENELLAEFNSAYRESNFDQLNEIAIILNHYNNGLNVIQSFINQHEYFVDTGEEEPLFDESFKSKITDMNYHGICYDKSMVTTLDEIESLIKNESKIVKKVFENRSAFVLQLFIQRIFAQKIEPRVGDLLNASLSLSNLAFVRMLYALHSLIGQFVKDLTEFFQFLSLDTEGDLVPTLERCFSDLFAKIIYDRSKYFDLEKRSLETALSLKTSTFSIAHDKEIFARALSNKLVNGATFSQDIDLQDYNASSNSKLSQINNFLKSHLERDKRPTYSDAPFQNLTEGGNANDSMRGATATDFSLQSIDSMLKCAIESIARVMELVPSKANEYTFEIVETVLMGTIGSYVEAGLEVAYTEMAEQDISKCADVNLSYLEYVSKASEMLSLISASVKTVVLPLFVNAPDVKKGIISLANHYIKKCELLINIMIEETIHGYTQRFAFSLSKQKKKDFLPKGQDILDQDTLPASEIVKDLHSLYSQISLYLKNENLKSFLRQVGNTLYRFLLEHYKKFQVSSVGGIIVTKDIIGIQTAVEEWSVEEILGDFTTLRELANLFTVQPEELSSLTSEGRLALVDRSTISAYIAKREDFNQESFVSKFKMHLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DL27</accession>
    <name>C5DL27_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0F09460p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0F09460g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the putative lipase ROG1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554615.1">
        <property type="nucleotide sequence ID" value="XM_002554569.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DL27"/>
    <dbReference type="FunCoup" id="C5DL27">
        <property type="interactions" value="36"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DL27"/>
    <dbReference type="GeneID" id="8292822"/>
    <dbReference type="KEGG" id="lth:KLTH0F09460g"/>
    <dbReference type="eggNOG" id="KOG4372">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007367_1_0_1"/>
    <dbReference type="InParanoid" id="C5DL27"/>
    <dbReference type="OMA" id="CFITADQ"/>
    <dbReference type="OrthoDB" id="5368485at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007751">
        <property type="entry name" value="DUF676_lipase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044294">
        <property type="entry name" value="Lipase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016445">
        <property type="entry name" value="Rog1_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482:SF62">
        <property type="entry name" value="LIPASE ROG1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482">
        <property type="entry name" value="LIPASE ROG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05057">
        <property type="entry name" value="DUF676"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005412">
        <property type="entry name" value="UCP005412_abhydr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF676" evidence="4">
        <location>
            <begin position="184"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="575"/>
            <end position="601"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR24178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="668" mass="75420" checksum="396478878F2AAF3B" modified="2009-07-28" version="1">MTQDGILYHAKSSVKVGEVERYVITYQLYDEDEGLPADLSLNSLWLKVRNLENFTFRAAYLMGPYILYCDVRTEDYHHSQRLFVSADQPKFEPMLQPQQDFIAELSLHNLKKTYVWVVDVVSQIIFTNTTQIGFEVSIGSSKKCLSKSSDCDPMVGSCSPKLNVTKLNTLDIWNLPNQLSTHASQKEHLVLLTHGLHSNVPADLFYLKEQIEKCQKYYADEQIVVKGYDKNVCKTEKGIKYLGTRMAEYIVNELYHDRVVKISFIGHSLGGLVQTFAIAYISVKYPWFFQKVEPINFITLASPLLGIVTDNPAYVNILLSFGIVGKTGQDLGLKESGKDGKPLLYCLPGEPTKRILKMFKRRTLYANAVNDGIVPLYSASLLFADYEGVVNQLKGMPEFQDKLRTTAPGDYIKRNFLSPLTKAISIWAPQKFPEETESKLPKVSVIESATSVLIPPLPDKAYVMDPSSRENVILHDRIYSEKDIPPRNSQSEKDLIESENVLLKAFTMSSEKHNQTLEEDIARKWHEGLVWRKVIVGLKPDAHNNIIVRRRFANAYGWPIIDHLTQNHFNGAESETAAHGEAQDRGTSSSPVQENDENKDIGWLDGISKPSMFDVGPTGMISTVGEIIDSLSKNNFLSKKSTTVHDLPAGPFEQQDLFSYEMEEKDLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLC6</accession>
    <name>C5DLC6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">General negative regulator of transcription subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0F11880g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the CNOT2/3/5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554714.1">
        <property type="nucleotide sequence ID" value="XM_002554668.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLC6"/>
    <dbReference type="FunCoup" id="C5DLC6">
        <property type="interactions" value="184"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLC6"/>
    <dbReference type="GeneID" id="8292928"/>
    <dbReference type="KEGG" id="lth:KLTH0F11880g"/>
    <dbReference type="eggNOG" id="KOG2150">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013819_3_0_1"/>
    <dbReference type="InParanoid" id="C5DLC6"/>
    <dbReference type="OMA" id="FIFYHYQ"/>
    <dbReference type="OrthoDB" id="293823at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
        <property type="term" value="C:CCR4-NOT core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000289">
        <property type="term" value="P:nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1020">
        <property type="entry name" value="CCR4-NOT complex subunit 2/3/5, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038635">
        <property type="entry name" value="CCR4-NOT_su2/3/5_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012270">
        <property type="entry name" value="CCR4-NOT_su3/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040168">
        <property type="entry name" value="Not2/3/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007282">
        <property type="entry name" value="NOT2/3/5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007207">
        <property type="entry name" value="Not_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23326">
        <property type="entry name" value="CCR4 NOT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04153">
        <property type="entry name" value="NOT2_3_5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04065">
        <property type="entry name" value="Not3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005290">
        <property type="entry name" value="NOT_su_3_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0010">Activator</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0678">Repressor</keyword>
    <keyword evidence="8 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CCR4-Not complex component Not N-terminal" evidence="13">
        <location>
            <begin position="2"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="NOT2/NOT3/NOT5 C-terminal" evidence="14">
        <location>
            <begin position="408"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="218"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="3"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="128"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="244"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="287"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="316"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR005290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF04153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR24277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="563" mass="65624" checksum="E718A08C7F3F6B5A" modified="2009-07-28" version="1">MSQRKLQQDIEKLLKKVKEGLQDFEDVYDKFQSTDASNTSYREKLESDLKREIKKLQKHRDQIKTWLSKEDVKDKQQVLMENRRLIESGMERFKSVEKMMKTKQFSTEALTNPDIIKDPRELKKRDQFLFVQDCLEELQKQLESYEAQDNEEQCERHAFHIANLENILKQLQNNELEPDTVEEYQEDIQYYVENNDDPDFIEYDTIYEDMDCEIQPGEEAKDLEPSSKPVKASKAERSPRKKGSSGAALPPSASNSLTDNQNSPSAVASPSTSSTGLASLPAKRDTPPQSQAPGQSQGQNSNEEQHPDHNSDLTPQQNSSLDQDQNRSQNQNQQQQNVNQTQPQTEAQPPRDMSKQIDEMLAQDRSQNSAFQNPLFHPGLDYWLESKRTLLQPYETMPAEMASQLESSLLNCPDSLDADTPHLYHNVLSLPHPTSIFFPNEPIRLVSNEPVDPTKTHPKHNDIYSRTSLARIMSKFDLDTLFFIFYHYQGTYEQFLSARELSIRGWQFNKVNRCWFYKEVEKLPPGMEQSEEISWRYFDYQKSWLARRCGAEFVYREEEFERI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLT8</accession>
    <name>C5DLT8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0G03432p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0G03432g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the purine-cytosine permease (2.A.39) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555186.1">
        <property type="nucleotide sequence ID" value="XM_002555140.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLT8"/>
    <dbReference type="FunCoup" id="C5DLT8">
        <property type="interactions" value="633"/>
    </dbReference>
    <dbReference type="GeneID" id="8294192"/>
    <dbReference type="KEGG" id="lth:KLTH0G03432g"/>
    <dbReference type="eggNOG" id="KOG2466">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021555_2_2_1"/>
    <dbReference type="InParanoid" id="C5DLT8"/>
    <dbReference type="OMA" id="WGSFWPI"/>
    <dbReference type="OrthoDB" id="2018619at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015205">
        <property type="term" value="F:nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11482">
        <property type="entry name" value="SLC-NCS1sbd_NRT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.4160.10:FF:000001">
        <property type="entry name" value="Uracil permease, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4160.10">
        <property type="entry name" value="Hydantoin permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012681">
        <property type="entry name" value="NCS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001248">
        <property type="entry name" value="Pur-cyt_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045225">
        <property type="entry name" value="Uracil/uridine/allantoin_perm"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00800">
        <property type="entry name" value="ncs1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30618:SF2">
        <property type="entry name" value="ALLANTOIN PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30618">
        <property type="entry name" value="NCS1 FAMILY PURINE/PYRIMIDINE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02133">
        <property type="entry name" value="Transp_cyt_pur"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="204"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="230"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="276"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="316"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="363"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="404"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="434"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="517"/>
            <end position="536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR24749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="604" mass="67920" checksum="539E189AD42B642C" modified="2009-07-28" version="1">MNQKSKTGDKSDAIISNSEQSSDVGQVHDKSENWWTRILTFLEVQKSTREETIKETFLYNFDLRPVEKKRRVWSWFNFVYFWLADCFNINTWQVAATGLQLGLNWWETWLTVWIGYAIPALLIVAIARIGSSYHVSFPIAARASFGVFFSLWPVINRVVMAIVWYSVQTWIAASPISLMLKSIFGYNLPNRIPDHISTPNATTYEFMCFFIFWVCQLPFVYVHPHQIRHLFTVKAALIPFAAFGFLIWALVKSDGKIALDSLVEGPQLTSSERGWAFMRSVLVCIGNFAALIINAPDFSRFSKTKLSATWSQALSVPFFFSITSLIGIIVTSAAYKLYSVNYWSPLDVLDRFLGDGFTRGERAGVFLISLIFAIAQLGTNISANSLSAGTDMTALLPKYINIRRGGFICAALALCITPWNLMSSSNKFTSALSAYAIFLSCIAAVMVADYYVVRRGKIEIQHLFCANKEVSIYMYGNRFGINWRALVAYVVGIAPNMPGFVSTVGDNIHVSAGALRLYYLNYLVGFSISFVVYVGLCRFFPVPGAPVANCLQKSEWMELYPEVEYFEEDREAFFADYSLGVVSSRLGEGDKGDITSAFAVRSQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLU5</accession>
    <name>C5DLU5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">L15</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">YL23</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0G03630g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the large ribosomal subunit (LSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes).</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the universal ribosomal protein uL11 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555193.1">
        <property type="nucleotide sequence ID" value="XM_002555147.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLU5"/>
    <dbReference type="FunCoup" id="C5DLU5">
        <property type="interactions" value="1113"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLU5"/>
    <dbReference type="GeneID" id="8293459"/>
    <dbReference type="KEGG" id="lth:KLTH0G03630g"/>
    <dbReference type="eggNOG" id="KOG0886">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074237_5_0_1"/>
    <dbReference type="InParanoid" id="C5DLU5"/>
    <dbReference type="OMA" id="QPPHDVI"/>
    <dbReference type="OrthoDB" id="1478556at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00349">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.250:FF:000002">
        <property type="entry name" value="60S ribosomal protein L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1550.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.250">
        <property type="entry name" value="Ribosomal protein L11, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1550.10">
        <property type="entry name" value="Ribosomal protein L11/L12, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00736">
        <property type="entry name" value="Ribosomal_uL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000911">
        <property type="entry name" value="Ribosomal_uL11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020783">
        <property type="entry name" value="Ribosomal_uL11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036769">
        <property type="entry name" value="Ribosomal_uL11_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020785">
        <property type="entry name" value="Ribosomal_uL11_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020784">
        <property type="entry name" value="Ribosomal_uL11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036796">
        <property type="entry name" value="Ribosomal_uL11_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00298">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03946">
        <property type="entry name" value="Ribosomal_L11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00649">
        <property type="entry name" value="RL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54747">
        <property type="entry name" value="Ribosomal L11/L12e N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46906">
        <property type="entry name" value="Ribosomal protein L11, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00359">
        <property type="entry name" value="RIBOSOMAL_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 9" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL11 N-terminal" evidence="11">
        <location>
            <begin position="14"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL11 C-terminal" evidence="10">
        <location>
            <begin position="74"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00064933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00080111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00082757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR24756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="165" mass="17808" checksum="2A48F5C5B23D36EA" modified="2009-07-28" version="1">MPPKFDPNEVKFLYLRAVGGEVGASAALAPKIGPLGLSPKKVGEDIAKATKEFKGIKVTVQLRIQNRQATASVVPSASSLVITALKEPPRDRKKEKNVKHSGNLQLEDIIEIAKQMKDKSFGKNLASVAKEILGTAQSVGCRVNYKNPHDIIEAIDAGEIEIPEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLX4</accession>
    <name>C5DLX4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphatidylinositol N-acetylglucosaminyltransferase subunit GPI19</fullName>
            <ecNumber evidence="7">2.4.1.198</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0G04268g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Part of the complex catalyzing the transfer of N-acetylglucosamine from UDP-N-acetylglucosamine to phosphatidylinositol, the first step of GPI biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + UDP-N-acetyl-alpha-D-glucosamine = a 6-(N-acetyl-alpha-D-glucosaminyl)-1-(1,2-diacyl-sn-glycero-3-phospho)-1D-myo-inositol + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14789"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57265"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="EC" id="2.4.1.198"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the phosphatidylinositol N-acetylglucosaminyltransferase (GPI-GlcNAc transferase) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the GPI19 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.198" evidence="7"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555222.1">
        <property type="nucleotide sequence ID" value="XM_002555176.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLX4"/>
    <dbReference type="FunCoup" id="C5DLX4">
        <property type="interactions" value="51"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLX4"/>
    <dbReference type="GeneID" id="8293488"/>
    <dbReference type="KEGG" id="lth:KLTH0G04268g"/>
    <dbReference type="eggNOG" id="KOG2257">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081616_4_0_1"/>
    <dbReference type="InParanoid" id="C5DLX4"/>
    <dbReference type="OMA" id="RYWIICI"/>
    <dbReference type="OrthoDB" id="690928at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017176">
        <property type="term" value="F:phosphatidylinositol N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052263">
        <property type="entry name" value="GPI_Anchor_Biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013717">
        <property type="entry name" value="PIG-P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016542">
        <property type="entry name" value="PIG-P_GPI19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46346">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46346:SF1">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08510">
        <property type="entry name" value="PIG-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF008765">
        <property type="entry name" value="PIG-P_GPI19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3 7" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="6 7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="64"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="PIG-P" evidence="9">
        <location>
            <begin position="8"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR008765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR24785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="146" mass="16993" checksum="67DA6BB5541B3BA5" modified="2009-07-28" version="1">MVKGVPRREYFGFSQFIFTTCILLLTFVWSLAPTLSAFKTEDASIRNEIITFTQELVGLLPQRYWIIVFECVVLMAMLFTYLGLWMYNEDVLTAPLDDMRTITDSRANVVKFASHQEFLDKYAFRESSGVMDLPITEVCRVLYEKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>C5DM03</accession>
    <name>C5DM03_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0G04906p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0G04906g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555251.1">
        <property type="nucleotide sequence ID" value="XM_002555205.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DM03"/>
    <dbReference type="GeneID" id="8293517"/>
    <dbReference type="KEGG" id="lth:KLTH0G04906g"/>
    <dbReference type="HOGENOM" id="CLU_2606453_0_0_1"/>
    <dbReference type="InParanoid" id="C5DM03"/>
    <dbReference type="OMA" id="MEWSESI"/>
    <dbReference type="OrthoDB" id="4032961at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="79" mass="8872" checksum="2725FFB46476573A" modified="2009-07-28" version="1">MSDARDFLRVTKNAGARHVVSGALGFVVGVSLASFVAVRIVDRTRKELEWTDSMTELTRSVRNLETHVKNLEHDYAGRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DM41</accession>
    <name>C5DM41_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0G05786p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0G05786g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555289.1">
        <property type="nucleotide sequence ID" value="XM_002555243.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DM41"/>
    <dbReference type="FunCoup" id="C5DM41">
        <property type="interactions" value="152"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DM41"/>
    <dbReference type="GeneID" id="8293556"/>
    <dbReference type="KEGG" id="lth:KLTH0G05786g"/>
    <dbReference type="eggNOG" id="KOG1577">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_0_1_1"/>
    <dbReference type="InParanoid" id="C5DM41"/>
    <dbReference type="OMA" id="PWCMRQE"/>
    <dbReference type="OrthoDB" id="416253at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19071">
        <property type="entry name" value="AKR_AKR1-5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000002">
        <property type="entry name" value="2,5-diketo-D-gluconic acid reductase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827">
        <property type="entry name" value="2,5-DIKETO-D-GLUCONIC ACID REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827:SF13">
        <property type="entry name" value="ALDO_KETO REDUCTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00798">
        <property type="entry name" value="ALDOKETO_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00063">
        <property type="entry name" value="ALDOKETO_REDUCTASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="5">
        <location>
            <begin position="22"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="4">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR24852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="286" mass="32667" checksum="0EAD97EBA0A06118" modified="2009-07-28" version="1">MSSTSVPRAYKLSSGYVLPSLGLGTYDIPRAETAEVVYQALKAGYRHLDTAVLYGNEREVGEGVEKWLKEDPKNHRRHDVFYTTKLWNSQNGYSNAKRAIQECLDKVRPLGYIDMLLIHSPLSGTRRRLETYKAMQEAVDAGIVRSIGVSNYGKHHIDELLAWEELKHKPVVNQIEISPWVMRQDLADYCKSKGLVVEAFSPLTHGYKIKHPNVVRVAEEVGRNTGQVLIRWSLQHGYVPLPKSRSVERLKGNLEVYDFELSPEQITFLDQPQAYEPTDWECTNEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5DM88</accession>
    <name>C5DM88_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Transcription elongation factor 1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0G06864g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Transcription elongation factor implicated in the maintenance of proper chromatin structure in actively transcribed regions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the ELOF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555336.1">
        <property type="nucleotide sequence ID" value="XM_002555290.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DM88"/>
    <dbReference type="FunCoup" id="C5DM88">
        <property type="interactions" value="705"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DM88"/>
    <dbReference type="GeneID" id="8293605"/>
    <dbReference type="KEGG" id="lth:KLTH0G06864g"/>
    <dbReference type="eggNOG" id="KOG3214">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105983_0_3_1"/>
    <dbReference type="InParanoid" id="C5DM88"/>
    <dbReference type="OMA" id="HEKSITC"/>
    <dbReference type="OrthoDB" id="445983at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008023">
        <property type="term" value="C:transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.190:FF:000001">
        <property type="entry name" value="Transcription elongation factor 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007808">
        <property type="entry name" value="Elf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038567">
        <property type="entry name" value="T_Elf1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20934">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20934:SF0">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05129">
        <property type="entry name" value="Zn_ribbon_Elf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="82"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="88"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="115"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR24899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="147" mass="16232" checksum="A5888DCBFC2FD349" modified="2009-07-28" version="1">MGKRKKSSRGPAKRVVQKLDLTFNCLFCNHEKSITCTLDKKNGIGSLSCKVCGQNFQTHINALSQPVDVYSDWFDAVEEVNAGKVSESEGESDSASDYESDSDAEQVDKRRAQIDSDEEEISEDEDAIGSSKRGRGKIVDSDEDEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMH0</accession>
    <name>C5DMH0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0G08844p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0G08844g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555418.1">
        <property type="nucleotide sequence ID" value="XM_002555372.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMH0"/>
    <dbReference type="FunCoup" id="C5DMH0">
        <property type="interactions" value="199"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMH0"/>
    <dbReference type="GeneID" id="8293694"/>
    <dbReference type="KEGG" id="lth:KLTH0G08844g"/>
    <dbReference type="eggNOG" id="ENOG502RFNU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023053_0_0_1"/>
    <dbReference type="InParanoid" id="C5DMH0"/>
    <dbReference type="OMA" id="MFLPNNI"/>
    <dbReference type="OrthoDB" id="5054775at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031930">
        <property type="term" value="P:mitochondria-nucleus signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006808">
        <property type="term" value="P:regulation of nitrogen utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013860">
        <property type="entry name" value="AreA_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053043">
        <property type="entry name" value="Ras-cAMP_regulatory"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28014">
        <property type="entry name" value="NEGATIVE REGULATOR OF RAS-CAMP PATHWAY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28014:SF1">
        <property type="entry name" value="NEGATIVE REGULATOR OF RAS-CAMP PATHWAY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08550">
        <property type="entry name" value="GATA_AreA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitrogen regulatory protein areA GATA-like" evidence="2">
        <location>
            <begin position="60"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="191"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="258"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="457"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="540"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="118"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="199"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="230"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="343"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR24981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="582" mass="64650" checksum="157E80443798E1CD" modified="2009-07-28" version="1">MMEETRSKSASGKHQPSKSKEPSNDPQSNSEADLSRQKFLKVAPSLFTPEKLPLFDSLDLYTALIRSSKALEQGERLHNLSWRIMNKALLKDHELNKSKKRDGVRNLYQVLNPANSGQFQRQQQPRQIPTPPRTHQRTASEMQPMQVMSAMQGTASEAKTSPEQPRFPHIHHQKLPVRATSDLQIKRGLHRAQDPRPGHQPQQPHLQQLHVQHAQSKHAPNKQGARFTLGNDSSVSSPESSQMILASPDATVFAGKREGSAQANSMDASDAQKRKKNHKRVAQPTRSAGSLFTPRPDDGPTASALPTKAISHDHIPKSLFGRKPTAHHKPAAPSSENKVFFSSDDDESDWNSLSDDSELYDDEEDDQYYQKQWDKLMFSRSEQSKGGFHAGRNTTPPPADKHDPKRSLLSGLFLNELANKAPTPPIMTPLTAQHPTLGESSVVAVGDVTPASLRASTSSFFPEGTQEKAPEPSAPSAIPPRYPRSRGSFSSIISDSTLQRYAHQSNAPPTAQTILPTALSTHMFLPNNVHQQRMARSVLSRSALSERRESIDIPSKNRNNSFLKTRMEISEEEEFARAKTGR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-07-27" modified="2025-06-18" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMU9</accession>
    <name>RKM5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosomal lysine N-methyltransferase 5</fullName>
            <ecNumber evidence="1">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">RKM5</name>
        <name type="ordered locus">KLTH0G11792g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">S-adenosyl-L-methionine-dependent protein-lysine N-methyltransferase that methylates 60S ribosomal protein L1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class I-like SAM-binding methyltransferase superfamily. RKM5 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555547.1">
        <property type="nucleotide sequence ID" value="XM_002555501.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMU9"/>
    <dbReference type="SMR" id="C5DMU9"/>
    <dbReference type="FunCoup" id="C5DMU9">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMU9"/>
    <dbReference type="GeneID" id="8293826"/>
    <dbReference type="KEGG" id="lth:KLTH0G11792g"/>
    <dbReference type="eggNOG" id="KOG1018">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051532_0_0_1"/>
    <dbReference type="InParanoid" id="C5DMU9"/>
    <dbReference type="OMA" id="ACDTIYN"/>
    <dbReference type="OrthoDB" id="2529286at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614:SF109">
        <property type="entry name" value="RIBOSOMAL LYSINE N-METHYLTRANSFERASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000411043" description="Ribosomal lysine N-methyltransferase 5">
        <location>
            <begin position="1"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="155"/>
            <end position="157"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q12367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q9H867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="334" mass="37244" checksum="F59CEF392A98F780" modified="2009-07-28" version="1">MTAKLKLLDEDGIYEHLFERYSLLQKHANELKQDLGIVSRSSGDLEVSFEPQPGSHVKEHFNFEIAQSLSSLNSSRDNNNSTTGYVVWSTTPFFLQWLLYSPSGAIFGKGGTIEVEGDASHSAYELPAIFGSRTVDTDESSDVPAAPQHIIVELGAGIAGMLCVALANYVDKYVCTDQKGLLNGLKRNIKHNIDELRLRNMESSTLDFEISRRTALKTELDVLDLDWESFGLKSSNFHTLITPAGPSTVCILSMDVVYNEFLIAPYLRTLKKLLQTYEKSGNTSFAILGIQLRDQDVVEMFLSTAVVQFELKVCAIVDSEIDKTRFGLYYITTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMV5</accession>
    <name>C5DMV5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0G11968p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0G11968g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555553.1">
        <property type="nucleotide sequence ID" value="XM_002555507.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMV5"/>
    <dbReference type="FunCoup" id="C5DMV5">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="GeneID" id="8293834"/>
    <dbReference type="KEGG" id="lth:KLTH0G11968g"/>
    <dbReference type="eggNOG" id="KOG3511">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_553283_0_0_1"/>
    <dbReference type="InParanoid" id="C5DMV5"/>
    <dbReference type="OrthoDB" id="4066654at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006892">
        <property type="term" value="P:post-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031778">
        <property type="entry name" value="Sortilin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006581">
        <property type="entry name" value="VPS10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050310">
        <property type="entry name" value="VPS10-sortilin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12106">
        <property type="entry name" value="SORTILIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12106:SF27">
        <property type="entry name" value="SORTILIN-RELATED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15902">
        <property type="entry name" value="Sortilin-Vps10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00602">
        <property type="entry name" value="VPS10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110296">
        <property type="entry name" value="Oligoxyloglucan reducing end-specific cellobiohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <feature type="domain" description="VPS10" evidence="4">
        <location>
            <begin position="6"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR25116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="493" mass="54236" checksum="3A8D1BCEDC1492C3" modified="2009-07-28" version="1">MQVHPSLVTGEGEESNSDGQRFFSIKSNLSLKQDLLLIVTNFRNVPSSGIVSETERKVFSSSDGENFRVARFYSFDANFHCNYFGSGNQNKIICQSPAFQRMEANTLMDVEGRILYSSDGGQTLQKIHHFDNLHIVSSHVSGDFHIVLTKNSPVKPAREFQIWVSRDLQSYELVSNALPIVEEPGTIKPLANSSRVLLRVPTSTESGKREVSLMLSNSNLSAFAPVLTFPSLKETHFSIENANGEIAVVTFTPGLSGPDKSVASRSNITFDEGKSWSKLCLEDFEAVQFGCSDAEPEACSLEVSRTIEIMGTYGRESTFPISAFVGYIAKQSDEGASVSEPMTFASKNGGKSWKKLLDFSAKLVVGGSGNIIDAFPDRRHVDSVPIFYSTDQGSTWSEVVLEESARHYLLIPTSTDGSSSVFGVVCYNPERGSANFYILNFSERTGQENSPDHGAGYIKGFEIDNQEVLQSLPDRQNGFLSGILEHLNQRLFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNA8</accession>
    <name>C5DNA8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0G15488p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0G15488g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="6">Homodimer. Forms heterodimer with DCS2; the interaction inhibits the DCS1 scavenger decapping activity during post-diauxic growth.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">P-body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Perinuclear region</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HIT family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555706.1">
        <property type="nucleotide sequence ID" value="XM_002555660.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNA8"/>
    <dbReference type="FunCoup" id="C5DNA8">
        <property type="interactions" value="828"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNA8"/>
    <dbReference type="GeneID" id="8293992"/>
    <dbReference type="KEGG" id="lth:KLTH0G15488g"/>
    <dbReference type="eggNOG" id="KOG3969">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041045_0_1_1"/>
    <dbReference type="InParanoid" id="C5DNA8"/>
    <dbReference type="OMA" id="RAYFHYQ"/>
    <dbReference type="OrthoDB" id="10264956at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000340">
        <property type="term" value="F:RNA 7-methylguanosine cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007584">
        <property type="term" value="P:response to nutrient"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006970">
        <property type="term" value="P:response to osmotic stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.40:FF:000002">
        <property type="entry name" value="m7GpppX diphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.428.10:FF:000015">
        <property type="entry name" value="m7GpppX diphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.40">
        <property type="entry name" value="Scavenger mRNA decapping enzyme, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008594">
        <property type="entry name" value="DcpS/DCS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019808">
        <property type="entry name" value="Histidine_triad_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011145">
        <property type="entry name" value="Scavenger_mRNA_decap_enz_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12978">
        <property type="entry name" value="HISTIDINE TRIAD HIT PROTEIN MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12978:SF0">
        <property type="entry name" value="M7GPPPX DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05652">
        <property type="entry name" value="DcpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11969">
        <property type="entry name" value="DcpS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028973">
        <property type="entry name" value="Scavenger_mRNA_decap_enz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102860">
        <property type="entry name" value="mRNA decapping enzyme DcpS N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00892">
        <property type="entry name" value="HIT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="274"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="265"/>
            <end position="276"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR028973-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR028973-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR25269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="364" mass="41801" checksum="764096F14914FC41" modified="2009-07-28" version="1">MSAPTAFGSEQPLRLQQLLSRFKFRRVLDSNPQTKVISLLGRIDGKDAIVTMEKMHFSYETNVFKKEEGRNTPVLHNCEAEFSCLKSIEEMREIASNDIYYWGTSVLKQDLAANPTAKINLIWPATPVHIRKFEAQSFHVIRETPAIYRAVVEPYVQEMCSENRLKWVHNILYNGAESERVVYKDFAEQEVPEGFLVLPDMKWDGVNLDSLYLVAIAFRSDIRSLRDLRPEHKPWLVKVLNKIRTVIPGCYNYAIRPDELRIFVHYQPSYYHFHIHIVNVKHSGLGDGISAGKAILLEDIIEQLNFLGSEGFMGRTITYVLGENHDLWKRGMEAAVLDQLEKDGIPKLPKAVNDFSVENLPGLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNB6</accession>
    <name>C5DNB6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein uS9m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">37S ribosomal protein S9, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0G15664g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uS9 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555714.1">
        <property type="nucleotide sequence ID" value="XM_002555668.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNB6"/>
    <dbReference type="FunCoup" id="C5DNB6">
        <property type="interactions" value="224"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNB6"/>
    <dbReference type="GeneID" id="8294000"/>
    <dbReference type="KEGG" id="lth:KLTH0G15664g"/>
    <dbReference type="eggNOG" id="KOG1697">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036531_0_0_1"/>
    <dbReference type="InParanoid" id="C5DNB6"/>
    <dbReference type="OMA" id="RESAMWA"/>
    <dbReference type="OrthoDB" id="10254627at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000001">
        <property type="entry name" value="30S ribosomal protein S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000754">
        <property type="entry name" value="Ribosomal_uS9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023035">
        <property type="entry name" value="Ribosomal_uS9_bac/plastid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020574">
        <property type="entry name" value="Ribosomal_uS9_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001099">
        <property type="entry name" value="PRK00132.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569">
        <property type="entry name" value="RIBOSOMAL PROTEIN S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US9M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00380">
        <property type="entry name" value="Ribosomal_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00360">
        <property type="entry name" value="RIBOSOMAL_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="260"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00039318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00042623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR25277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="279" mass="31596" checksum="9582136015D6B37B" modified="2009-07-28" version="1">MFSRLCNAARLAVSHGGRHCRNFSNYAHLSKDYTLQQTRIVPKLTTFYSANPHHEDRIDRLESLLRKYIKLPTVQMTPSEKPAWLSFSEYALIGGGTRLKPIQYQQLVNLLNRLQAIDPELINDEITSELSQYFKKTKLQSSQISIKTLDEFGRSVAVGRRKASTSKVYLVRGSGEVLVNGRQLNDYFVKMKDRESVAYPLKVVEGAGKYNAFVTTSGGGVTGQAEATMHAIAKALLVFNPLLKPRLQKAGVLTRDYRHVERKKPGKKKARKMPTWVKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNB9</accession>
    <name>C5DNB9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Glyceraldehyde-3-phosphate dehydrogenase</fullName>
            <ecNumber evidence="14">1.2.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">KLTH0G15730g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = (2R)-3-phospho-glyceroyl phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10300"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="1.2.1.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 14">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.12" evidence="14"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555717.1">
        <property type="nucleotide sequence ID" value="XM_002555671.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNB9"/>
    <dbReference type="FunCoup" id="C5DNB9">
        <property type="interactions" value="1420"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNB9"/>
    <dbReference type="GeneID" id="8294003"/>
    <dbReference type="KEGG" id="lth:KLTH0G15730g"/>
    <dbReference type="eggNOG" id="KOG0657">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030140_0_3_1"/>
    <dbReference type="InParanoid" id="C5DNB9"/>
    <dbReference type="OMA" id="YGYTCNM"/>
    <dbReference type="OrthoDB" id="1152826at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00184"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030312">
        <property type="term" value="C:external encapsulating structure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004365">
        <property type="term" value="F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd18126">
        <property type="entry name" value="GAPDH_I_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05214">
        <property type="entry name" value="GAPDH_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000001">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000020">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020831">
        <property type="entry name" value="GlycerAld/Erythrose_P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020830">
        <property type="entry name" value="GlycerAld_3-P_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020829">
        <property type="entry name" value="GlycerAld_3-P_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020828">
        <property type="entry name" value="GlycerAld_3-P_DH_NAD(P)-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006424">
        <property type="entry name" value="Glyceraldehyde-3-P_DH_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01534">
        <property type="entry name" value="GAPDH-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10836">
        <property type="entry name" value="GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10836:SF76">
        <property type="entry name" value="GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02800">
        <property type="entry name" value="Gp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00044">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000149">
        <property type="entry name" value="GAP_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00078">
        <property type="entry name" value="G3PDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00846">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00071">
        <property type="entry name" value="GAPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 14" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="7 11 14" id="KW-0520">NAD</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyceraldehyde 3-phosphate dehydrogenase NAD(P) binding" evidence="15">
        <location>
            <begin position="3"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="9">
        <location>
            <position position="150"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="12"/>
            <end position="13"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="149"/>
            <end position="151"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="209"/>
            <end position="210"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="314"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="site" description="Activates thiol group during catalysis" evidence="12">
        <location>
            <position position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAR25280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="332" mass="35403" checksum="9C2E861695E8AB5F" modified="2009-07-28" version="1">MVTTVAINGFGRIGRLVLRIALSRKDLKVAAINDPFIAVEYAAYMFKYDSTHGRYAGEVSHEGSNLIIDGKKIAVFQEKDPAQLPWGKLGVDIAIDSTGVFKELDSAQKHIDAGAKKVVITAPSSTAPMFVVGVNEKEYKGQNIVSNASCTTNCLAPLAKVINDEFGIEEGLMTTVHSLTATQKTVDGPSHKDWRGGRTASGNIIPSSTGAAKAVGKVLPVLQGKLTGMAFRVPTVDVSVVDLTVKLAKETTYDQIKAAVKKASEGSMKGVLGYTEEAVVSTDFVGDSHSSIFDASAGIQLSPKFVKLVSWYDNEYGYSTRVVDLVEHVASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNJ6</accession>
    <name>C5DNJ6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0G17578p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0G17578g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ACBP family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555794.1">
        <property type="nucleotide sequence ID" value="XM_002555748.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNJ6"/>
    <dbReference type="FunCoup" id="C5DNJ6">
        <property type="interactions" value="437"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNJ6"/>
    <dbReference type="GeneID" id="8294087"/>
    <dbReference type="KEGG" id="lth:KLTH0G17578g"/>
    <dbReference type="eggNOG" id="KOG0817">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118853_4_1_1"/>
    <dbReference type="InParanoid" id="C5DNJ6"/>
    <dbReference type="OMA" id="RYKFEAW"/>
    <dbReference type="OrthoDB" id="346910at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000062">
        <property type="term" value="F:fatty-acyl-CoA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000010">
        <property type="entry name" value="Acyl-CoA-binding domain-containing protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022408">
        <property type="entry name" value="Acyl-CoA-binding_prot_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000582">
        <property type="entry name" value="Acyl-CoA-binding_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035984">
        <property type="entry name" value="Acyl-CoA-binding_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23310:SF62">
        <property type="entry name" value="ACYL-COA BINDING PROTEIN 1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23310">
        <property type="entry name" value="ACYL-COA-BINDING PROTEIN, ACBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00887">
        <property type="entry name" value="ACBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00689">
        <property type="entry name" value="ACOABINDINGP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47027">
        <property type="entry name" value="Acyl-CoA binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00880">
        <property type="entry name" value="ACB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51228">
        <property type="entry name" value="ACB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACB" evidence="3">
        <location>
            <begin position="2"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR25357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="86" mass="9804" checksum="8573F10568B2E2F6" modified="2009-07-28" version="1">MVSATFEQKAEAVKNLATTPSNDELLQLYGLYKQATVGDNSSPRPSALNFKDKYKWDAWEALKGTSQEEAEQKYIELVDQLQQKYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2023-06-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNT6</accession>
    <name>C5DNT6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0G19734p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0G19734g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555884.1">
        <property type="nucleotide sequence ID" value="XM_002555838.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNT6"/>
    <dbReference type="GeneID" id="8294181"/>
    <dbReference type="KEGG" id="lth:KLTH0G19734g"/>
    <dbReference type="HOGENOM" id="CLU_2590181_0_0_1"/>
    <dbReference type="InParanoid" id="C5DNT6"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR25447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="80" mass="9230" checksum="418B8004A32DE981" modified="2009-07-28" version="1">MPAFEVSSKILLNLPIMPSKPLKKTPKVYVTSLVQRTRLQEYQPFTNPILSCLELNFMKLENCLQNCMLSRKLGALRFSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5E265</accession>
    <name>C5E265_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">homocitrate synthase</fullName>
            <ecNumber evidence="6">2.3.3.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">KLTH0H02486g</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the aldol-type condensation of 2-oxoglutarate with acetyl-CoA to yield homocitrate. Carries out the first step of the alpha-aminoadipate (AAA) lysine biosynthesis pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>acetyl-CoA + 2-oxoglutarate + H2O = (2R)-homocitrate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12929"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:58884"/>
            <dbReference type="EC" id="2.3.3.14"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:12930"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-alpha-aminoadipate from 2-oxoglutarate: step 1/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the alpha-IPM synthase/homocitrate synthase family. Homocitrate synthase LYS20/LYS21 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.14" evidence="6"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555988.1">
        <property type="nucleotide sequence ID" value="XM_002555942.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E265"/>
    <dbReference type="FunCoup" id="C5E265">
        <property type="interactions" value="609"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E265"/>
    <dbReference type="GeneID" id="8294301"/>
    <dbReference type="KEGG" id="lth:KLTH0H02486g"/>
    <dbReference type="eggNOG" id="KOG2367">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022158_2_2_1"/>
    <dbReference type="InParanoid" id="C5E265"/>
    <dbReference type="OMA" id="NTMRMLV"/>
    <dbReference type="OrthoDB" id="2015253at2759"/>
    <dbReference type="UniPathway" id="UPA00033">
        <property type="reaction ID" value="UER00028"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004410">
        <property type="term" value="F:homocitrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd07948">
        <property type="entry name" value="DRE_TIM_HCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.260:FF:000002">
        <property type="entry name" value="Homocitrate synthase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000032">
        <property type="entry name" value="Homocitrate synthase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02222">
        <property type="entry name" value="Homocitr_synth_fung_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050073">
        <property type="entry name" value="2-IPM_HCS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002034">
        <property type="entry name" value="AIPM/Hcit_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048253">
        <property type="entry name" value="DRE_TIM_HCS_fun_bact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011872">
        <property type="entry name" value="Homocitrate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054691">
        <property type="entry name" value="LeuA/HCS_post-cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02146">
        <property type="entry name" value="LysS_fung_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10277:SF48">
        <property type="entry name" value="HOMOCITRATE SYNTHASE, CYTOSOLIC ISOZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10277">
        <property type="entry name" value="HOMOCITRATE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22617">
        <property type="entry name" value="HCS_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00815">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00816">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0464">Manganese</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="16">
        <location>
            <begin position="36"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="414"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAR30126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="440" mass="48557" checksum="34CF8D3BFEC95382" modified="2009-07-28" version="1">MSNSGFQQVTESTTQVERPNPYGPNPADYLSNVANFQLIDSTLREGEQFANAFFTTEKKIEIAKALDDFGVDYIELTSPVASEQSRSDCEAICKLGLKAKILTHIRCHMDDAKVAVETGVDGVDVVIGTSKFLRQYSHGKDMNYIAKSAIEVIEFVKSKGIEIRFSSEDSFRSDLVDLLNIYKTVDSIGVNRVGIADTVGCANPRQVYELLRTLKTVVHCDVECHFHNDTGCAIANAYTALEGGARLIDVAVLGIGERNGITPLGGLMARMIVAAPEYVKSKYKLHKIRDIENLVAEAVEVNIPFNNPITGFCAFTHKAGIHAKAILANPSTYEILNPHDFGLKRYIHFANRLTGWNAIKSRVDQLNLNMSDEQCKEVTMKIKKLGDIRPLSIDDVDSIIKDFHSEITTPQLRNMDAQGSDDLNNLDISAPAAKKAKKSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5E276</accession>
    <name>C5E276_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0H02728p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0H02728g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Allantoate permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555999.1">
        <property type="nucleotide sequence ID" value="XM_002555953.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E276"/>
    <dbReference type="FunCoup" id="C5E276">
        <property type="interactions" value="41"/>
    </dbReference>
    <dbReference type="GeneID" id="8294312"/>
    <dbReference type="KEGG" id="lth:KLTH0H02728g"/>
    <dbReference type="eggNOG" id="KOG2533">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_4_2_1"/>
    <dbReference type="InParanoid" id="C5E276"/>
    <dbReference type="OMA" id="WNIFCWN"/>
    <dbReference type="OrthoDB" id="3639251at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17327">
        <property type="entry name" value="MFS_FEN2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000065">
        <property type="entry name" value="Putative MFS pantothenate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF15">
        <property type="entry name" value="TRANSPORTER SEO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="130"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="180"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="204"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="234"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="266"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="300"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="373"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="409"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="438"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="466"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="498"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="532"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="39"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="54"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR30137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="584" mass="67351" checksum="B119BD41BC773DF2" modified="2009-07-28" version="1">MEVVKTSLTNFSKEWVVEPYARLKWGFIPVRRIVDENEVESEEEHSEQVVEEAFSDKQVSDDRKTELEVEQKSSVSNSGGAGEMEYRDEANRKWWSFFNEQEYRMPQKRANSAKWWQWFGSDVPAQEKKLLLKLDILLAFYSCMAYWVKYLDTANLNNAYVSGLKEELNFKGNELVETQAMYTVGNIVFQLPFIFFLDKVPLNYVLPALDLCWSILTIGASQVDSVPHLKAIRFFIGVFEAPSYLAYQYLFGSFYKHDEMVRRSAFYYLGQYAGILSASGIQSAIYAAMDGKHGLSGWRWNFIVDGLISVVVGLIGFYSLPGDPHNCYSIFLTDDEIRLARKRLRENNTDKSDFSSKVLDKAVWKRIFTNWRIYVLTLWNIFCWNNNNGTSGAYLLWVKSLKKYSVAKVNQLGMITPALGMVYLFGTGVFADKLHSRWGAIIVTQIFNFIGNVILAVWAVPESAKWFAFMLQYFGWAMAPVLYSWQNDICRRDSQARAITLVVMNMLAQTTTAFMSVLVWKTTEAPRYLKGYTWTAVCAFCLSAWTILVLWLYKRDEKQHAEENGIIIYNSKTGENYPPEKQNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2E1</accession>
    <name>C5E2E1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">cAMP-dependent protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0H04180g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.11"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.11" evidence="2"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556064.1">
        <property type="nucleotide sequence ID" value="XM_002556018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2E1"/>
    <dbReference type="FunCoup" id="C5E2E1">
        <property type="interactions" value="893"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2E1"/>
    <dbReference type="GeneID" id="8294377"/>
    <dbReference type="KEGG" id="lth:KLTH0H04180g"/>
    <dbReference type="eggNOG" id="KOG0616">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_63_5_1"/>
    <dbReference type="InParanoid" id="C5E2E1"/>
    <dbReference type="OMA" id="NDPFFDW"/>
    <dbReference type="OrthoDB" id="63267at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004691">
        <property type="term" value="F:cAMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010607">
        <property type="term" value="P:negative regulation of cytoplasmic mRNA processing body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010737">
        <property type="term" value="P:protein kinase A signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05580">
        <property type="entry name" value="STKc_PKA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000005">
        <property type="entry name" value="cAMP-dependent protein kinase catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000005">
        <property type="entry name" value="cAMP-dependent protein kinase catalytic subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353:SF153">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE CATALYTIC SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="43"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="14">
        <location>
            <begin position="298"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR30202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="353" mass="40777" checksum="81E36823ACAB70E3" modified="2009-07-28" version="1">MEYQNEGYGHSVVPDRPGMGERQRSLLPQRSAVSKGKYSLNDFQILRTLGTGSFGRVHLVRSVHNGRYYAIKVLKKEQVIRMKQIEHTNDERRMLKLVDHPFLIRMWGTFQDSRNLFMVMDYIEGGELFSLLRKSQRFPNPVAKFYAAEVILALEYLHAHDIIYRDLKPENILLDRNGHIKITDFGFAKEVDTVTWTLCGTPDYIAPEVIATKPYNKSVDWWSLGILIFEMLAGYTPFYDATPMKTYEKILQGKVAYPPFFHPDVVDLLSKLITADLTRRLGNLQSGPQDVKSHPWFSEVIWEKLLAKDIETPYEPPITAGVGDSSLFDQYPEEQLDYGSQGDDPFMQYFIDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2I4</accession>
    <name>C5E2I4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0H05236p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0H05236g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556107.1">
        <property type="nucleotide sequence ID" value="XM_002556061.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2I4"/>
    <dbReference type="STRING" id="559295.C5E2I4"/>
    <dbReference type="GeneID" id="8294422"/>
    <dbReference type="KEGG" id="lth:KLTH0H05236g"/>
    <dbReference type="eggNOG" id="KOG0773">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068284_0_0_1"/>
    <dbReference type="InParanoid" id="C5E2I4"/>
    <dbReference type="OrthoDB" id="10056939at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050224">
        <property type="entry name" value="TALE_homeobox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11850">
        <property type="entry name" value="HOMEOBOX PROTEIN TRANSCRIPTION FACTORS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 4" id="KW-0371">Homeobox</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="6">
        <location>
            <begin position="100"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="4">
        <location>
            <begin position="102"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="54"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="63"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR30245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="234" mass="25933" checksum="4AE32EC4FA2A3F75" modified="2009-07-28" version="1">MNSNAQCFAQLEQGVKLPSIRSLLDSIGAEQEGGAETRRPLPALALPALASTLPERQQQPDNQLSQPRNQQPGQQPNNLQLSHPHNQQPSQQAPSAARRAAAPPRRNNLPKETVEILNAWLASHLNNPYPSPQEKRELLVQTGLSKVQLSNWFINVRRRKVFSDYYQMSRARRAGAPDEATDAVGAAGAPTATDADLELRFQAAPLTRRKKLIDRLDELKKASQDSARQCRGPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2N5</accession>
    <name>C5E2N5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0H06402p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0H06402g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556158.1">
        <property type="nucleotide sequence ID" value="XM_002556112.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2N5"/>
    <dbReference type="FunCoup" id="C5E2N5">
        <property type="interactions" value="257"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2N5"/>
    <dbReference type="GeneID" id="8294473"/>
    <dbReference type="KEGG" id="lth:KLTH0H06402g"/>
    <dbReference type="eggNOG" id="ENOG502QRP4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011913_0_0_1"/>
    <dbReference type="InParanoid" id="C5E2N5"/>
    <dbReference type="OMA" id="HIHPTKH"/>
    <dbReference type="OrthoDB" id="10256176at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="2">
        <location>
            <begin position="557"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="320"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="428"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="42"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="327"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="345"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="433"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="488"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR30296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="752" mass="83129" checksum="FBA9833C6C0D34A6" modified="2009-07-28" version="1">MEGKKKQHSKGELECTDGSRRNSGFVPGVGKALGVSDSSKNTKSPSWTSSISTITEELPAPKVSEAMTRVPSAASAVSYPYCSSVPEEPGAKPCPTRARWWNTLPKIGETVHSHKHSLNAVVLNVNGTPREIIYDPRYNPLFSKLEIFYMFNAQTPLSDCYTYAKTRLASFVKFLEVYHSSRQYASACLPFNVSVAANATAGGYPSYTAGVDYHEIGEIVQLWYLQSLEFMIDNNSFLFSNEIVDYLVNYEPQKNASIKRELSCSGPPPSALQETIIRADILLIRCTFEEELGWQLALDEPNWNIVDLFVDLSFLGRLTTEDEGEDSSGNATSTTADVDGDTTLSPPSSASSSPATSATTSSSDVSYGKVSESVMREPVRSGNDIFVDPQTDVPETSSARACHVIVQDCVGRDLDKLAAEMRAKGINETCRPSISQNSSQESFSSQKQGQSGKKDQSTKSAKSSTGLGISNFFRRKNSHASGDKGDNPLSSKRAPQSDSVKMNLTIQNSYLDDYYASTLANFRKLVLPSHCMFTRREAPKPFKRENGASKKQEANSYQKEFLQIKIPLKDESFPVIICPDVWFSLEFKKWKGLINELFRCIRPGGFLETSACNLTNVNDGYDIEKSSKEFPTLLEMKALNDVISMEAVKAGLQVFPMRHLVGALKAAGFVNIKHSVFSLKRGDLRHNMGFLFEFLAIRNYDYQLRNDLLSSEVKPPGTNPASFPLRYVEEHMGKADEDAGVVRLVLITAQKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2P3</accession>
    <name>C5E2P3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 18">Pyruvate carboxylase</fullName>
            <ecNumber evidence="7 18">6.4.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="ordered locus">KLTH0H06600g</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="28">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.</text>
    </comment>
    <comment type="function">
        <text evidence="3">Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17 18">
            <text>hydrogencarbonate + pyruvate + ATP = oxaloacetate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20844"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.4.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 18">
            <name>biotin</name>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="6.4.1.1" evidence="7 18"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556166.1">
        <property type="nucleotide sequence ID" value="XM_002556120.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2P3"/>
    <dbReference type="FunCoup" id="C5E2P3">
        <property type="interactions" value="754"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2P3"/>
    <dbReference type="GeneID" id="8294482"/>
    <dbReference type="KEGG" id="lth:KLTH0H06600g"/>
    <dbReference type="eggNOG" id="KOG0369">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000395_0_1_1"/>
    <dbReference type="InParanoid" id="C5E2P3"/>
    <dbReference type="OMA" id="AEACICY"/>
    <dbReference type="OrthoDB" id="196847at2759"/>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004736">
        <property type="term" value="F:pyruvate carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd06850">
        <property type="entry name" value="biotinyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07937">
        <property type="entry name" value="DRE_TIM_PC_TC_5S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000003">
        <property type="entry name" value="Acetyl-CoA carboxylase biotin carboxyl carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000018">
        <property type="entry name" value="Biotin carboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000010">
        <property type="entry name" value="Propionyl-CoA carboxylase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000600">
        <property type="entry name" value="Pyruvate carboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.600.10:FF:000007">
        <property type="entry name" value="Pyruvate carboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000033">
        <property type="entry name" value="Pyruvate carboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000012">
        <property type="entry name" value="Pyruvate carboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.600.10">
        <property type="entry name" value="pyruvate carboxylase f1077a mutant domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005481">
        <property type="entry name" value="BC-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001882">
        <property type="entry name" value="Biotin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011764">
        <property type="entry name" value="Biotin_carboxylation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005482">
        <property type="entry name" value="Biotin_COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003379">
        <property type="entry name" value="Carboxylase_cons_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055268">
        <property type="entry name" value="PCB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005930">
        <property type="entry name" value="Pyruv_COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006761">
        <property type="entry name" value="PRK09282.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009554">
        <property type="entry name" value="PRK12999.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01235">
        <property type="entry name" value="pyruv_carbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43778">
        <property type="entry name" value="PYRUVATE CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43778:SF2">
        <property type="entry name" value="PYRUVATE CARBOXYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02785">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00289">
        <property type="entry name" value="Biotin_carb_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02436">
        <property type="entry name" value="PYC_OADA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001594">
        <property type="entry name" value="Pyruv_carbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00878">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89000">
        <property type="entry name" value="post-HMGL domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50979">
        <property type="entry name" value="BC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00188">
        <property type="entry name" value="BIOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="14 18 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15 18" id="KW-0092">Biotin</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="10 18" id="KW-0436">Ligase</keyword>
    <keyword evidence="11 21" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="12 18 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Biotin carboxylation" evidence="25">
        <location>
            <begin position="16"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="24">
        <location>
            <begin position="138"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="26">
        <location>
            <begin position="555"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoyl-binding" evidence="23">
        <location>
            <begin position="1092"/>
            <end position="1167"/>
        </location>
    </feature>
    <feature type="active site" evidence="19">
        <location>
            <position position="310"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="564"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="636"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="via carbamate group" evidence="21">
        <location>
            <position position="732"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="762"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="764"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="896"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-carboxylysine" evidence="22">
        <location>
            <position position="732"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-biotinyllysine" evidence="22">
        <location>
            <position position="1133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRNR" id="PIRNR001594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR001594-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR001594-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR001594-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR001594-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EMBL" id="CAR30304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1174" mass="128924" checksum="D5B9EB62401BF9E2" modified="2009-07-28" version="1">MSKLAGLRDSSNLLGEKNKLLVANRGEIPIRIFRSAHELSMKTVAIYSHEDRLSMHRLKADEAYMIGKEGKYTPVGAYLAIDEILKIAKEHNVDFIHPGYGFLSENSEFAQKVEAAGITWIGPSAEVIESVGDKVSARNLAAKADVPVVPGTPGPIDSVEEAQAFVEEYGYPVIIKAAYGGGGRGMRVVREGDDIADAFQRATSEAKTAFGNGTCFIERFLNKPKHIEVQLLADGYGNVVHLFERDCSVQRRHQKVVEVAPAKTLPKDVRDAILTDAVKLAKVAGYKNAGTAEFLVDDQNRHYFIEINPRIQVEHTITEEITGIDIVAAQIQIAAGASLQELGLLQDKIITRGFAIQCRITTEDPAKNFQPDTGRIEVYRSAGGNGVRLDGGNAYAGSIISPHYDSMLVKCSCSGSTYEIVRRKMLRALIEFRIRGVKTNIPFLLTLLTNPVFIDGSYWTTFIDDTPELFKMVSSQNRAQKLLHYLADLAVNGSSIKGQMGLPKLHTQPSVPTLHDSNGEVIDVLTTPPPSGWRQVLLEQGPAQFAKAVRHYKGTLLMDTTWRDAHQSLLATRVRTYDLAAIAPTTAHALSGAFALECWGGATFDVAMRFLHEDPWERLRILRKLVPNIPFQMLLRGANGVAYSSLPDNAIDHFVEQAKENGVDIFRVFDALNDLEQLKVGVDAVKKANGVVEATICYSGDMLQPGKKYNLDYYLEITDKIVQMGTHILGIKDMAGTLKPSAAKLLIGSIRAKYPDLPIHVHTHDSAGTAVASMAACAFAGADVVDVATNSMSGMTSQPSINALLASLDGEIDTGVNVNMVRELDAYWAQMRLLYSCFEADLKGPDPEVYEHEIPGGQLTNLLFQAQQLGLGEKWAETKRAYRDANYLLGDLVKVTPTSKVVGDLAQFMVSNKLTPDDVRRLASSLDFPDSVMDFFEGLIGQPYGGFPEPLRSDVLKNKRRKLTCRPGLELAPFDLAGIKEDLQDRFGDIDECDVASYNMYPKVYEDFRKMRETYGDLSVLPTKNFLSPPTVGEEIVVTIEKGKTLIIKPQAIGELNKETGLREVYFDLNGELRKVSVIDKSQKVDTLSKPKADAHDPFQIGAPMAGVIIEVKVHKGSLVKKGQPIAVLSAMKMEMVISSPTDGQVKDVLVKDGENVDASDLLVFLEESVPPKE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-06-28" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2Q7</accession>
    <name>HRI1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName>Protein HRI1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">HRI1</name>
        <name type="ordered locus">KLTH0H06930g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HRI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556180.1">
        <property type="nucleotide sequence ID" value="XM_002556134.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2Q7"/>
    <dbReference type="SMR" id="C5E2Q7"/>
    <dbReference type="FunCoup" id="C5E2Q7">
        <property type="interactions" value="32"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2Q7"/>
    <dbReference type="GeneID" id="8294497"/>
    <dbReference type="KEGG" id="lth:KLTH0H06930g"/>
    <dbReference type="eggNOG" id="ENOG502QTYD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097607_0_0_1"/>
    <dbReference type="InParanoid" id="C5E2Q7"/>
    <dbReference type="OMA" id="GEVNTTW"/>
    <dbReference type="OrthoDB" id="4045395at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd11693">
        <property type="entry name" value="HRI1_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11692">
        <property type="entry name" value="HRI1_N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.310">
        <property type="entry name" value="Protein HRI1, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.320">
        <property type="entry name" value="Protein HRI1, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031818">
        <property type="entry name" value="Hri1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038744">
        <property type="entry name" value="Hri1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043047">
        <property type="entry name" value="Hri1_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16815">
        <property type="entry name" value="HRI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0539">Nucleus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000410811" description="Protein HRI1">
        <location>
            <begin position="1"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="261" mass="29287" checksum="105A483EFBAE5B65" modified="2009-07-28" version="1">MCSEHPLAVKWQSKIIAMVSLNKRCTFQVGENPADERTLTLSSASNGGYYISLRPFVEPKGSESELPYEWAFAGSPKSLEAKKVDELRDDINFNFDFDTNVHLNAENTHRGVVETHWKKWESGLVEERGQVFPFGPDKQAVSFFELWQPIDATRDEFVHISEKSVDQSAARSVVLALDDDNYRGLVITVGKWTQGILFKKAEHTAKGINFLRACESADGKITTLLKYGVDFDRFPSTFVGQKDAELQGAAGAFWKVVESNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2X3</accession>
    <name>C5E2X3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0H08492p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0H08492g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NASP family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556246.1">
        <property type="nucleotide sequence ID" value="XM_002556200.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2X3"/>
    <dbReference type="STRING" id="559295.C5E2X3"/>
    <dbReference type="GeneID" id="8294567"/>
    <dbReference type="KEGG" id="lth:KLTH0H08492g"/>
    <dbReference type="eggNOG" id="KOG4563">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028900_0_0_1"/>
    <dbReference type="InParanoid" id="C5E2X3"/>
    <dbReference type="OMA" id="IAECHYK"/>
    <dbReference type="OrthoDB" id="5587616at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034080">
        <property type="term" value="P:CENP-A containing chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006335">
        <property type="term" value="P:DNA replication-dependent chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051730">
        <property type="entry name" value="NASP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019544">
        <property type="entry name" value="Tetratricopeptide_SHNi-TPR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15081:SF1">
        <property type="entry name" value="NUCLEAR AUTOANTIGENIC SPERM PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15081">
        <property type="entry name" value="NUCLEAR AUTOANTIGENIC SPERM PROTEIN NASP -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10516">
        <property type="entry name" value="SHNi-TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="Tetratricopeptide SHNi-TPR" evidence="9">
        <location>
            <begin position="170"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="6">
        <location>
            <begin position="170"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="69"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="295"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="236"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="119"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="131"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="322"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF10516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR30384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="333" mass="37272" checksum="607B64C9381E551B" modified="2009-07-28" version="1">MGGREEIKHLLAEAAKFYAARDYEKSTNLYSEVNALHDTLTGTSSADYLFLYGKSLFQLALSQSEVFGKDEETAGGESDSDEEESSKKHELYQFSETLAEGDEIEKGDDAEQDDEEVEGEHPGENEHEGAGDEGQEEEPSDFENAWEILELARSVYEKQPQEPETLRKLSETYDILGEISLETENFPQAKQDFEKCLELRQAVYGTVDATHRLIIESYYKLSLVLEFDPNDAKDCQENLSKAVELLEKRIKEKGAEGGDDGLLEELKLKLKELKTTEASLNAIKHQSMAQIKEALGLPSDSKSAPAAVNDLTSMVKKRKSKPEKDTASKKQKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2Z4</accession>
    <name>C5E2Z4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Ras-related protein RSR1</fullName>
            <ecNumber evidence="3">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0H09042g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Ras family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="3"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556267.1">
        <property type="nucleotide sequence ID" value="XM_002556221.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2Z4"/>
    <dbReference type="FunCoup" id="C5E2Z4">
        <property type="interactions" value="285"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2Z4"/>
    <dbReference type="GeneID" id="8294592"/>
    <dbReference type="KEGG" id="lth:KLTH0H09042g"/>
    <dbReference type="eggNOG" id="KOG0395">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041217_9_0_1"/>
    <dbReference type="InParanoid" id="C5E2Z4"/>
    <dbReference type="OMA" id="MPLREFK"/>
    <dbReference type="OrthoDB" id="5976022at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000114">
        <property type="term" value="P:regulation of establishment of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04177">
        <property type="entry name" value="RSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000631">
        <property type="entry name" value="Ras small monomeric GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041841">
        <property type="entry name" value="Rsr1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020849">
        <property type="entry name" value="Small_GTPase_Ras-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24070">
        <property type="entry name" value="RAS, DI-RAS, AND RHEB FAMILY MEMBERS OF SMALL GTPASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0636">Prenylation</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="190"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="192"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="227"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00072720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR30405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="242" mass="26669" checksum="15020BF5EAE5FEE8" modified="2009-07-28" version="1">MRDYKLVVLGAGGVGKSCLTVQFVQGVYLDTYDPTIEDSYRKTMEIDNKVFDLEILDTAGVAQFTAMRELYIKSGMGFLLVYSVTDRQSLEELIELREQVLRIKDSTRVPMVLVGNKADLQDERVISVEEGIEMSSNWGKVPFYETSALLRSNVDEVFVDAVRQIIRNEIEAQNSSTAALGHMSRKNTGLGAASANAADPSAAQDTPKKQVTLNRPATAKQAPGKPSTKRKTKKTKTSCVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5E340</accession>
    <name>C5E340_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">DNA replication complex GINS protein PSF2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0H10164g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="6">Component of the GINS complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the GINS2/PSF2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556313.1">
        <property type="nucleotide sequence ID" value="XM_002556267.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E340"/>
    <dbReference type="FunCoup" id="C5E340">
        <property type="interactions" value="732"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E340"/>
    <dbReference type="GeneID" id="8294643"/>
    <dbReference type="KEGG" id="lth:KLTH0H10164g"/>
    <dbReference type="eggNOG" id="KOG4071">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078274_1_1_1"/>
    <dbReference type="InParanoid" id="C5E340"/>
    <dbReference type="OMA" id="DSLNCMY"/>
    <dbReference type="OrthoDB" id="1938138at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000811">
        <property type="term" value="C:GINS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11712">
        <property type="entry name" value="GINS_A_psf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21694">
        <property type="entry name" value="GINS_B_Psf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1020:FF:000001">
        <property type="entry name" value="DNA replication complex GINS protein PSF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021151">
        <property type="entry name" value="GINS_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036224">
        <property type="entry name" value="GINS_bundle-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007257">
        <property type="entry name" value="GINS_Psf2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056784">
        <property type="entry name" value="PSF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12772">
        <property type="entry name" value="DNA REPLICATION COMPLEX GINS PROTEIN PSF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12772:SF0">
        <property type="entry name" value="DNA REPLICATION COMPLEX GINS PROTEIN PSF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25005">
        <property type="entry name" value="PSF2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05916">
        <property type="entry name" value="Sld5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028998">
        <property type="entry name" value="GINS_Psf2_subgr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158573">
        <property type="entry name" value="GINS helical bundle-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160059">
        <property type="entry name" value="PriA/YqbF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA replication complex GINS protein PSF2 N-terminal" evidence="8">
        <location>
            <begin position="60"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="GINS subunit" evidence="7">
        <location>
            <begin position="91"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR028998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF25005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR30451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="209" mass="24251" checksum="2DE84109399C4331" modified="2009-07-28" version="1">MSLPWNIQETFSPEEIQFIVENEPIKIFPRFTTRVSLRGDRAKPAHTRWKLVTADDHPLNNLVAIQTTEVALWMALLLKQQGKCSIVAPGWLRLSQLQKYIDYELKHAERFSELPWNWLVVAQLLFSKAADDLHDPVHLLRGKIQDLREIRLGKIGQGLKHLNESHLQLDNLSLLEINELRPFVLGVMNKLKQVHSSISEERGSEDYYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5E370</accession>
    <name>C5E370_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cysteine synthase 1</fullName>
            <ecNumber evidence="5">2.5.1.47</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">O-acetylserine (thiol)-lyase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">O-acetylserine sulfhydrylase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">O-succinylserine sulfhydrylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ordered locus">KLTH0H10846g</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the conversion of O-succinyl-L-serine into cysteine, the last step in the cysteine biosynthesis pathway. Can also use O-acetyl-L-serine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate</text>
            <dbReference type="Rhea" id="RHEA:14829"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:58340"/>
            <dbReference type="EC" id="2.5.1.47"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>O-succinyl-L-serine + hydrogen sulfide = L-cysteine + succinate</text>
            <dbReference type="Rhea" id="RHEA:53816"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:136856"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cysteine synthase/cystathionine beta-synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.47" evidence="5"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556343.1">
        <property type="nucleotide sequence ID" value="XM_002556297.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E370"/>
    <dbReference type="STRING" id="559295.C5E370"/>
    <dbReference type="GeneID" id="8294674"/>
    <dbReference type="KEGG" id="lth:KLTH0H10846g"/>
    <dbReference type="eggNOG" id="KOG1481">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021018_1_0_1"/>
    <dbReference type="InParanoid" id="C5E370"/>
    <dbReference type="OMA" id="VVTVFWD"/>
    <dbReference type="OrthoDB" id="10259545at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004124">
        <property type="term" value="F:cysteine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01561">
        <property type="entry name" value="CBS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000011">
        <property type="entry name" value="Cysteine synthase (o-acetylserine)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050214">
        <property type="entry name" value="Cys_Synth/Cystath_Beta-Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001216">
        <property type="entry name" value="P-phosphate_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007989">
        <property type="entry name" value="PRK10717.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10314">
        <property type="entry name" value="CYSTATHIONINE BETA-SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00901">
        <property type="entry name" value="CYS_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0198">Cysteine biosynthesis</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="19">
        <location>
            <begin position="14"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00050981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00058228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00072087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00078262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00079147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00081847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CAR30481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="349" mass="37927" checksum="EE9914B5B5C4B3BE" modified="2009-07-28" version="1">MTQFPVLASKGLVDAVGKTPLIKLNTLSKELGRNIFGKAEFQNPGGSVKDRAALYLIEQAEKSGQIDPSRPATIVEGSAGNTAIGLAHIARSKGYKSIFYMPNTQSVSKINLLKYLGAEVYPVPVCPISDPMNFNNRARDHAKRLDNAIWTDQFDNPANWKSHYATTGPEILQQLQSAGLSCDAFTCSTGTGGTFTGVAKYLKEATNGQSKLVVADPPGSVIYSYIKTGEMNREGSSFTEGIGQGRITKNLRESIDITDDAVFVPDEESIVMLFRLLDEEGLFIGGTTALNVVAACKVAKTLPEGSNISTILCDSGHKYADRVFSKKWLREKKLYDAIPDSLKRYAILD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>C5E372</accession>
    <name>C5E372_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0H10890p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0H10890g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556345.1">
        <property type="nucleotide sequence ID" value="XM_002556299.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E372"/>
    <dbReference type="FunCoup" id="C5E372">
        <property type="interactions" value="238"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E372"/>
    <dbReference type="GeneID" id="8294676"/>
    <dbReference type="KEGG" id="lth:KLTH0H10890g"/>
    <dbReference type="eggNOG" id="KOG1658">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086758_1_0_1"/>
    <dbReference type="InParanoid" id="C5E372"/>
    <dbReference type="OMA" id="AKCDPEH"/>
    <dbReference type="OrthoDB" id="636685at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008623">
        <property type="term" value="C:CHRAC"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22929">
        <property type="entry name" value="HFD_POLE4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050568">
        <property type="entry name" value="Transcr_DNA_Rep_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10252:SF54">
        <property type="entry name" value="CHROMATIN ACCESSIBILITY COMPLEX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10252">
        <property type="entry name" value="HISTONE-LIKE TRANSCRIPTION FACTOR CCAAT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription factor CBF/NF-Y/archaeal histone" evidence="4">
        <location>
            <begin position="16"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="123"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="136"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="174"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR30483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="201" mass="21809" checksum="E44171C8C657D9F2" modified="2009-07-28" version="1">MEHSSSLEAVQKAAPRLPISKVKRIAKCDPEHMLTSQSATAAVAFATELFVQVFSEEGFARSQLERPAGARLTLRYDDLSACVASSDRFAFLADVVPETQSLVSLTCENRVRYTTLAPGQTVLPFRAKEEPANSDGDGEEEPDEQPEAELDAEIDEDGDNHVEAEIDVYTQNDADLEPELDAEADTDIKPTADPEAGAVVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3G2</accession>
    <name>C5E3G2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">KLTH0H13288p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0H13288g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the syntaxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556435.1">
        <property type="nucleotide sequence ID" value="XM_002556389.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3G2"/>
    <dbReference type="FunCoup" id="C5E3G2">
        <property type="interactions" value="809"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3G2"/>
    <dbReference type="GeneID" id="8294794"/>
    <dbReference type="KEGG" id="lth:KLTH0H13288g"/>
    <dbReference type="eggNOG" id="KOG0809">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="10251371at2759"/>
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        <property type="component" value="Chromosome H"/>
    </dbReference>
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        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:SNAP receptor activity"/>
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        <property type="term" value="F:SNARE binding"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15845">
        <property type="entry name" value="SNARE_syntaxin16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006012">
        <property type="entry name" value="Syntaxin/epimorphin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF83">
        <property type="entry name" value="SYNTAXIN-16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00914">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="300"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="12">
        <location>
            <begin position="226"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="322"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="322"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="342"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR30573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="356" mass="41151" checksum="CF3F386A4FB28605" modified="2009-07-28" version="1">MFRDRTNLFLSYRRTFPRNRYNNSDALTSFDLEENGVEESYPMIDSKKDVPTLSGQFLNLTQEVDRNLDEAGTKMLQLTKLYRKNALPGFEDKVSDEQEIEEHSYQIIKMFQQSYAIIKSLQSIQTAQTFRGQSLRKGDLVVVDNLQKHYASKIQSSSNKFRMLQNNYLKFLNKDDFKPLPRASTDGDALLVLEEEETNAATQQEIDSYSRQTLQRQTQRQSQGQSTQFLQREEEITQLARGVLEVSTIFREMQNLVIDQGTIIDRIDYNLENTVLELKGAQRELDRATVYQSRTQKCKVILLLSLVVITLFFFVMLKPHHHSESSKKDPPQPKQNSSQGEGKGEISDGDTKPKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3J0</accession>
    <name>C5E3J0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0H13970p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0H13970g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556463.1">
        <property type="nucleotide sequence ID" value="XM_002556417.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3J0"/>
    <dbReference type="FunCoup" id="C5E3J0">
        <property type="interactions" value="179"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3J0"/>
    <dbReference type="GeneID" id="8294825"/>
    <dbReference type="KEGG" id="lth:KLTH0H13970g"/>
    <dbReference type="eggNOG" id="KOG0395">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041217_9_8_1"/>
    <dbReference type="InParanoid" id="C5E3J0"/>
    <dbReference type="OMA" id="HQGHGKT"/>
    <dbReference type="OrthoDB" id="5976022at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001447">
        <property type="entry name" value="Ras-related protein Rab-1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020849">
        <property type="entry name" value="Small_GTPase_Ras-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24070">
        <property type="entry name" value="RAS, DI-RAS, AND RHEB FAMILY MEMBERS OF SMALL GTPASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR30601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="192" mass="20906" checksum="C90BF6B1C6C42B61" modified="2009-07-28" version="1">MEDPNTRMSKHRKIAVMGARNVGKSSLTVQFVESRFVESYYPTIENQFTKQVVLGKTAYTLEICDTAGQDEFSLINTKSLMGVKGVAIVYSCVNRASFEIVELIRDKILDQLGLDNVPTVIVANKIDLRDSGINGGRVDSREGAELAARLGAGFVECSAKLNIGVEDAVLQLLKRIEGIDGDLSDASKCSIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3K7</accession>
    <name>C5E3K7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0H14366g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="2"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556480.1">
        <property type="nucleotide sequence ID" value="XM_002556434.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3K7"/>
    <dbReference type="FunCoup" id="C5E3K7">
        <property type="interactions" value="85"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3K7"/>
    <dbReference type="GeneID" id="8294842"/>
    <dbReference type="KEGG" id="lth:KLTH0H14366g"/>
    <dbReference type="eggNOG" id="KOG0941">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002173_5_1_1"/>
    <dbReference type="InParanoid" id="C5E3K7"/>
    <dbReference type="OMA" id="MYYLFGA"/>
    <dbReference type="OrthoDB" id="8068875at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000003">
        <property type="entry name" value="probable E3 ubiquitin-protein ligase HERC4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044611">
        <property type="entry name" value="E3A/B/C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45700:SF8">
        <property type="entry name" value="HECT-TYPE E3 UBIQUITIN TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45700">
        <property type="entry name" value="UBIQUITIN-PROTEIN LIGASE E3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="HECT" evidence="6">
        <location>
            <begin position="514"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="5">
        <location>
            <position position="820"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR30618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="852" mass="98329" checksum="89098BE16D01F139" modified="2009-07-28" version="1">MVSISILKGKKKGTERPIEVTHHSYAGGRHEKTKRGTAGVKEVLTTCSCCGTPLRHPEVVKKIKCVKCHCTLELSLRTAQTPVQAAITPTTLTPQDFWDVSSACVLQYTNEPRHGTLDVDSKHGIFVPIEDFIQRLFANVSSLNAFCTNGTKELDFKCIGRFYKRIIELPTSRPLYKLLIESNERLKRPHVRLSPDAEPTSMFSQLYWVLMIFENPLLKNCLLFSANGEHSHFLTPQIRAILYEIMKRCVGYLAMVNSATGKDFVSHLQRLSIVHFGSHVDLINLYVTFHFSRILNKRAKSAACLDSPGLDEFCSNLKLNPENSGNDFETIPQMVSKLWRREDSRGVELPSSFKFHVAEYGNDWHVKTAAKLAFFYFVANQCKKKCPVSRFYNSLLDYLDHKKDFEQWRARQKLRSPSSSPLSTSMFEDILMPHYFGPSRRPETDFTMCQHAYLLSLGTKILVMEYETRKTMEYNAEQAFLKALDKKQVIDVYFRMRVRRDYVSQDSLRCIETHHADLKKSLRVEFVNEPGIDAGGLRKEWFLLLTRELFNPSSGLFIVVEESRFSWFNMAYKDVDFGPENAEKLYYLFGIVLGLAIYNGTILDLCFPMALYKKLCGEPLNERDFLELYPTTGKNLIKMMEYDGDDFEDVFCLNFEITYSNTWATRIHRQELCDGGAKRHVTRENRSEYVRLWMDFHMNRAIKSSFAVFLNGFRRVVESDAFKLFTSEELEQLLCGSHDRDIDVNVLKSVAKYGASITAESRIVRWFWDTFQDFNFEQKCKLLEFVTASDRIPATGISTIPFKISKLGGDSEKLPLVHTCFNEICLYDYNSQPKLRHKLILAMNESEGYAFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3N8</accession>
    <name>C5E3N8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Carbamoyl phosphate synthase arginine-specific small chain</fullName>
            <ecNumber evidence="3">6.3.5.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Arginine-specific carbamoyl phosphate synthetase, glutamine chain</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Glutamine-dependent carbamoyl phosphate synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">KLTH0H15114g</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Small subunit of the arginine-specific carbamoyl phosphate synthase (CPSase). CPSase catalyzes the formation of carbamoyl phosphate from the ammonia moiety of glutamine, carbonate, and phosphate donated by ATP, constituting the first step of 2 biosynthetic pathways, one leading to arginine and/or urea and the other to pyrimidine nucleotides. The small subunit (glutamine amidotransferase) binds and cleaves glutamine to supply the large subunit with the substrate ammonia.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>hydrogencarbonate + L-glutamine + 2 ATP + H2O = carbamoyl phosphate + L-glutamate + 2 ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:18633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Heterodimer composed of 2 chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CarA family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.5" evidence="3"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556511.1">
        <property type="nucleotide sequence ID" value="XM_002556465.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3N8"/>
    <dbReference type="FunCoup" id="C5E3N8">
        <property type="interactions" value="410"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3N8"/>
    <dbReference type="GeneID" id="8294876"/>
    <dbReference type="KEGG" id="lth:KLTH0H15114g"/>
    <dbReference type="eggNOG" id="KOG0370">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035901_1_1_1"/>
    <dbReference type="InParanoid" id="C5E3N8"/>
    <dbReference type="OMA" id="CFSVQYH"/>
    <dbReference type="OrthoDB" id="434at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004088">
        <property type="term" value="F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01744">
        <property type="entry name" value="GATase1_CPSase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000016">
        <property type="entry name" value="Carbamoyl-phosphate synthase arginine-specific small chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.20:FF:000003">
        <property type="entry name" value="Carbamoyl-phosphate synthase arginine-specific small chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.20">
        <property type="entry name" value="Carbamoyl-phosphate synthase small subunit, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01209">
        <property type="entry name" value="CPSase_S_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050472">
        <property type="entry name" value="Anth_synth/Amidotransfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006274">
        <property type="entry name" value="CarbamoylP_synth_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002474">
        <property type="entry name" value="CarbamoylP_synth_ssu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036480">
        <property type="entry name" value="CarbP_synth_ssu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035686">
        <property type="entry name" value="CPSase_GATase1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01368">
        <property type="entry name" value="CPSaseIIsmall"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009475">
        <property type="entry name" value="PRK12838.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43418:SF7">
        <property type="entry name" value="CARBAMOYL-PHOSPHATE SYNTHASE SMALL CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43418">
        <property type="entry name" value="MULTIFUNCTIONAL TRYPTOPHAN BIOSYNTHESIS PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00988">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00099">
        <property type="entry name" value="CPSGATASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00096">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01097">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52021">
        <property type="entry name" value="Carbamoyl phosphate synthetase, small subunit N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carbamoyl-phosphate synthase small subunit N-terminal" evidence="17">
        <location>
            <begin position="7"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00057756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM01097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAR30649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="399" mass="43482" checksum="19AFA0F71D1A17D7" modified="2009-07-28" version="1">MAPPVLTKATFQIQNGPSYDGYSFGAKASVAGETVFTTSLVGYPESMTDPSYKGQILVFTQPLIGNYGVPSGEARDEFNLLKYFESPHAHVVGIVVAEYAWRYSHWTAVQSLAEWCEKEGVAAITGVDTRSVVQYLREQGSSLGRIVVGDEAAPEYVDTMKSHLVAQVSTREPYHISNANATANLALIDCGVKENIVRCLASRGANVTVLPHDYKIQDVADQFDGIFISNGPGNPTSCGQTVENIKVLLEDPKFAHLPIFGICLGHQLLALAAGARSVKMKYGNRAHNIPALDLTTGQCHITSQNHGYAIDAESLPKNVFKPFFVNLNDGSNEGMIHCTRPVFSTQFHPEAKGGPMDTAVLFDKYFENIAQYQKTRDSSAKTAVKFNIPVQTMASQRVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3Q9</accession>
    <name>C5E3Q9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0H15598p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0H15598g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the large ribosomal subunit (LSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes). The 5 acidic ribosomal P-proteins form the stalk structure of the 60S subunit. They are organized as a pentameric complex in which uL10/P0 interacts with 2 heterodimers, P1A-P2B and P1B-P2A.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic ribosomal protein P1/P2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556532.1">
        <property type="nucleotide sequence ID" value="XM_002556486.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3Q9"/>
    <dbReference type="FunCoup" id="C5E3Q9">
        <property type="interactions" value="856"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3Q9"/>
    <dbReference type="GeneID" id="8294898"/>
    <dbReference type="KEGG" id="lth:KLTH0H15598g"/>
    <dbReference type="eggNOG" id="KOG1762">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114656_1_0_1"/>
    <dbReference type="InParanoid" id="C5E3Q9"/>
    <dbReference type="OMA" id="YSAHDHE"/>
    <dbReference type="OrthoDB" id="2194681at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030295">
        <property type="term" value="F:protein kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043021">
        <property type="term" value="F:ribonucleoprotein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006414">
        <property type="term" value="P:translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05831">
        <property type="entry name" value="Ribosomal_P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1410:FF:000002">
        <property type="entry name" value="60S acidic ribosomal protein P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01478">
        <property type="entry name" value="Ribosomal_L12_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038716">
        <property type="entry name" value="P1/P2_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027534">
        <property type="entry name" value="Ribosomal_P1/P2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45696">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45696:SF10">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00428">
        <property type="entry name" value="Ribosomal_60s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="71"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="71"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="84"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00063629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR30670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="107" mass="10871" checksum="56F0824D3A0D39CF" modified="2009-07-28" version="1">MSTETALSYAALILADSEVEISTEKLLALTDAANIQIEGIWADIFAKALDSQDLKSLLVNFSAGAAAAPTGGAAAGAAAGGDDAAAEEKEEEAKEESDDDMGFGLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3T2</accession>
    <name>C5E3T2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cofilin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Actin-depolymerizing factor 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0H16104g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the actin-binding proteins ADF family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556555.1">
        <property type="nucleotide sequence ID" value="XM_002556509.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3T2"/>
    <dbReference type="FunCoup" id="C5E3T2">
        <property type="interactions" value="860"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3T2"/>
    <dbReference type="GeneID" id="8294921"/>
    <dbReference type="KEGG" id="lth:KLTH0H16104g"/>
    <dbReference type="eggNOG" id="KOG1735">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094004_3_2_1"/>
    <dbReference type="InParanoid" id="C5E3T2"/>
    <dbReference type="OMA" id="QCRFAVY"/>
    <dbReference type="OrthoDB" id="10249245at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016363">
        <property type="term" value="C:nuclear matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030042">
        <property type="term" value="P:actin filament depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11286">
        <property type="entry name" value="ADF_cofilin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000060">
        <property type="entry name" value="Cofilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002108">
        <property type="entry name" value="ADF-H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017904">
        <property type="entry name" value="ADF/Cofilin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11913">
        <property type="entry name" value="COFILIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00241">
        <property type="entry name" value="Cofilin_ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00102">
        <property type="entry name" value="ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51263">
        <property type="entry name" value="ADF_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ADF-H" evidence="10">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR30693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="131" mass="14748" checksum="DEF97444A158004F" modified="2009-07-28" version="1">MKAFNDLKLGKKYKFVLYALNDNKTEIVVKETSTAQDYDAFLEKLSEDDCLYAVYDFEYEIGGNEGKRSKIVFFTWSPDTAPVRAKMVYASSKDALRRALNGISTDIQGTDYSEVAYESVLEKVSRGAGSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-02-15" modified="2025-10-08" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q5K465</accession>
    <name>Q5K465_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Putative DNA endonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">io-cox1.1</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <geneLocation type="mitochondrion" evidence="14"/>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2005" name="FEBS Lett." volume="579" first="30" last="40">
            <title>The complete mitochondrial genome of the yeast Kluyveromyces thermotolerans.</title>
            <authorList>
                <person name="Talla E."/>
                <person name="Anthouard V."/>
                <person name="Bouchier C."/>
                <person name="Frangeul L."/>
                <person name="Dujon B."/>
            </authorList>
            <dbReference type="PubMed" id="15620687"/>
            <dbReference type="DOI" id="10.1016/j.febslet.2004.10.106"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the LAGLIDADG endonuclease family.</text>
    </comment>
    <dbReference type="EMBL" id="AJ634268">
        <property type="protein sequence ID" value="CAG25599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_184722.1">
        <property type="nucleotide sequence ID" value="NC_006626.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q5K465"/>
    <dbReference type="FunCoup" id="Q5K465">
        <property type="interactions" value="9"/>
    </dbReference>
    <dbReference type="STRING" id="559295.Q5K465"/>
    <dbReference type="GeneID" id="3238978"/>
    <dbReference type="InParanoid" id="Q5K465"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Mitochondrion"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006314">
        <property type="term" value="P:intron homing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.28.10:FF:000007">
        <property type="entry name" value="Intron-encoded DNA endonuclease aI3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.28.10:FF:000011">
        <property type="entry name" value="Intron-encoded DNA endonuclease aI3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.210.10">
        <property type="entry name" value="Cytochrome c oxidase-like, subunit I domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.28.10">
        <property type="entry name" value="Homing endonucleases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023616">
        <property type="entry name" value="Cyt_c_oxase-like_su1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036927">
        <property type="entry name" value="Cyt_c_oxase-like_su1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000883">
        <property type="entry name" value="Cyt_C_Oxase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027434">
        <property type="entry name" value="Homing_endonucl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004860">
        <property type="entry name" value="LAGLIDADG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051289">
        <property type="entry name" value="LAGLIDADG_Endonuclease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36181">
        <property type="entry name" value="INTRON-ENCODED ENDONUCLEASE AI3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36181:SF6">
        <property type="entry name" value="INTRON-ENCODED LAGLIDADG ENDONUCLEASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00115">
        <property type="entry name" value="COX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00961">
        <property type="entry name" value="LAGLIDADG_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01165">
        <property type="entry name" value="CYCOXIDASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81442">
        <property type="entry name" value="Cytochrome c oxidase subunit I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55608">
        <property type="entry name" value="Homing endonucleases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50855">
        <property type="entry name" value="COX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 14" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0404">Intron homing</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="57"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="292"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome oxidase subunit I profile" evidence="13">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAG25599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="415" mass="48001" checksum="5921CAA5B0D2313F" modified="2005-02-15" version="1">MVQRWLYSTNAKDIAILYFIFAIFCGMAGTAMSVIIRLELAAPGNQYLGGNHQLFNVLVVGHAVLMIFFLVMPALIGGFGNQKRYESNNNNNQVMENKEYNLKLNYDKLGPYLAGLIEGDGTITVQNSSSMKKSKYRPLIVVVFKLEDLELANYLCNLTKCGKVYKKINRNYVLWTIHDLKGVYTLLNIINGYMRTPKYEAFVRCAEFMNNYINSTTITHNKLKNMDNIKIKPLDTSDIGSNAWLAGMTDADGNFSINLMNGKNRSSRAMPYYCLELRQNYHQNSNNNNINFSYFYIMSAIATYFNVNLYSRERNLNLLVSTNNTYKTYYSYKVMVANTYKNIKVMEYFNKYSLLSSKHLDFLDWSKLVILINNEGQSMKTNGSWELGMNLRKDYNKTRTTFTWSHLKNTYLENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBI7</accession>
    <name>C5DBI7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 8">Glucosamine 6-phosphate N-acetyltransferase</fullName>
            <ecNumber evidence="3 8">2.3.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0A02948g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 8">
            <text>D-glucosamine 6-phosphate + acetyl-CoA = N-acetyl-D-glucosamine 6-phosphate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10292"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57513"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="EC" id="2.3.1.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route I): step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the acetyltransferase family. GNA1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.4" evidence="3 8"/>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551586.1">
        <property type="nucleotide sequence ID" value="XM_002551540.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBI7"/>
    <dbReference type="FunCoup" id="C5DBI7">
        <property type="interactions" value="476"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBI7"/>
    <dbReference type="GeneID" id="8290385"/>
    <dbReference type="KEGG" id="lth:KLTH0A02948g"/>
    <dbReference type="eggNOG" id="KOG3396">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072095_0_1_1"/>
    <dbReference type="InParanoid" id="C5DBI7"/>
    <dbReference type="OMA" id="NQRYDWI"/>
    <dbReference type="OrthoDB" id="10039976at2759"/>
    <dbReference type="UniPathway" id="UPA00113">
        <property type="reaction ID" value="UER00529"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004343">
        <property type="term" value="F:glucosamine 6-phosphate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006048">
        <property type="term" value="P:UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000136">
        <property type="entry name" value="Glucosamine 6-phosphate N-acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039143">
        <property type="entry name" value="GNPNAT1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13355">
        <property type="entry name" value="GLUCOSAMINE 6-PHOSPHATE N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13355:SF11">
        <property type="entry name" value="GLUCOSAMINE 6-PHOSPHATE N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="9">
        <location>
            <begin position="8"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00069869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR21144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="157" mass="17556" checksum="93ADA2358652A5BC" modified="2009-07-28" version="1">MPVLPNEYSIRRVKPSDYEGIVETLRVLTVVGDVTKEQFEETVAYWNEVTVKSKGEPIKAYNPHVITDGSGKVVATGTVFIERKILHDCGLVGHIEDIAVAKDQQGKKLGQLLIEHLTELALSRGCYKVILDCDEKNVGFYQKCGYSIKGVEMDRRS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-05-31" modified="2025-06-18" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBS6</accession>
    <name>AIM9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName>Altered inheritance of mitochondria protein 9, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Found in mitochondrial proteome protein 29</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">AIM9</name>
        <name type="synonym">FMP29</name>
        <name type="ordered locus">KLTH0A05016g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the AIM9 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551675.1">
        <property type="nucleotide sequence ID" value="XM_002551629.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBS6"/>
    <dbReference type="FunCoup" id="C5DBS6">
        <property type="interactions" value="26"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBS6"/>
    <dbReference type="GeneID" id="8290479"/>
    <dbReference type="KEGG" id="lth:KLTH0A05016g"/>
    <dbReference type="eggNOG" id="ENOG502QV1E">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019189_0_1_1"/>
    <dbReference type="InParanoid" id="C5DBS6"/>
    <dbReference type="OMA" id="GWIPQDM"/>
    <dbReference type="OrthoDB" id="2968323at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051035">
        <property type="entry name" value="Mito_inheritance_9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36091">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36091:SF1">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0496">Mitochondrion</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0809">Transit peptide</keyword>
    <feature type="transit peptide" description="Mitochondrion" evidence="2">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000408724" description="Altered inheritance of mitochondria protein 9, mitochondrial">
        <location>
            <begin position="35"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="619" mass="70774" checksum="14E4BDA9439A5517" modified="2009-07-28" version="1" precursor="true">MLRYNLRIPVQAKKAMGSLAPLRPMKSRFGLRCISDKPNEVFTKLSDDNDPQRDAFFKYSWGSWLANDRQEKEKRTTKFSIEGLTDVINDLHAQSKEKAKSEASEGNIPPPSYNPNLTVSLSHNLSALTLGSLNPNETLRVTAMASIHEGKHHRIYKVDTNADKSFILRIPYATNSEAAISYRLKSEVATMDFADLKLGLKVPKVYCFGANALNPIRQPFILEEHIEGRLLMRDWNPLEDDASDKKAHISKLNKVIDPLSQFQSKLLSVEFNQFGSLYFAKDYPDSEGPAYEGEKNESLKNRWSVGPSVERCFWRQKSALPLDKLMQFVGPWSKSKPMDIVKSLGLLEAENAKSRLALQQTDASPAPIEESILREQVRTFENLAEISSSLFNTKSHVIPNIDSLLKPRLYHPDLDPMNVLLDEKDNSAPYLLDFENTSIKPFILQNSPQFVAYDGPKIYNLEEDVEGYKDLSEAEKVQYQFMYKRTRNQHLWESALNKNFNKLISAVAPPVKLLRSPYVACVERKNDMEYLLVDESLIQLREVWEVFAKNKLVSEKKFPLEYTEEQLQKHSTDLNAFHEQLIKSPFAATQGWIPQDMFENLVKAGILIKDKNGDYTIKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBZ0</accession>
    <name>C5DBZ0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0A06468p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0A06468g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551739.1">
        <property type="nucleotide sequence ID" value="XM_002551693.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBZ0"/>
    <dbReference type="FunCoup" id="C5DBZ0">
        <property type="interactions" value="37"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBZ0"/>
    <dbReference type="GeneID" id="8290545"/>
    <dbReference type="KEGG" id="lth:KLTH0A06468g"/>
    <dbReference type="eggNOG" id="KOG4530">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055322_2_0_1"/>
    <dbReference type="InParanoid" id="C5DBZ0"/>
    <dbReference type="OMA" id="NDHTKAW"/>
    <dbReference type="OrthoDB" id="68575at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005025">
        <property type="entry name" value="FMN_Rdtase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050712">
        <property type="entry name" value="NAD(P)H-dep_reductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30543">
        <property type="entry name" value="CHROMATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30543:SF21">
        <property type="entry name" value="NAD(P)H-DEPENDENT FMN REDUCTASE LOT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03358">
        <property type="entry name" value="FMN_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADPH-dependent FMN reductase-like" evidence="1">
        <location>
            <begin position="1"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="206" mass="23250" checksum="D08A70C1269ED234" modified="2009-07-28" version="1">MLIGVIIGSTRSPRVCPQIAQFILDTIERSKSYQELSEKPLLKIIDIADWNLPLYDESNVPSQIKRSEDYDHEHTKLWSLEIQKYNGFIFVTPQYNWGYPAALKNAIDYLFNEWKGKSAAIVTYGGHGGTKCNEQLAVVLEGLRMIPTQSRVLLSFPDRDTLYAAASGKDLGLSSNPSGDLWTTEKTQIEATFNELFESIVKTQDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5DC08</accession>
    <name>C5DC08_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0A06886p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0A06886g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551757.1">
        <property type="nucleotide sequence ID" value="XM_002551711.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DC08"/>
    <dbReference type="FunCoup" id="C5DC08">
        <property type="interactions" value="230"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DC08"/>
    <dbReference type="GeneID" id="8290564"/>
    <dbReference type="KEGG" id="lth:KLTH0A06886g"/>
    <dbReference type="eggNOG" id="KOG3145">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046327_0_0_1"/>
    <dbReference type="InParanoid" id="C5DC08"/>
    <dbReference type="OMA" id="HYILYPE"/>
    <dbReference type="OrthoDB" id="75720at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015184">
        <property type="term" value="F:L-cystine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005282">
        <property type="entry name" value="LC_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13131">
        <property type="entry name" value="CYSTINOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13131:SF5">
        <property type="entry name" value="CYSTINOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00679">
        <property type="entry name" value="CTNS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="79"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="121"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="182"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="226"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR21315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="249" mass="28464" checksum="DF6DB324DB596116" modified="2009-07-28" version="1">MNLESALGYGYVIPWSVSIYPVVWHNWRHRSASAMSIDFVIINVFGYLYLLLSLWLQLFCWQTGQEHEDSTRPKITAFDFYYCLHGVVMNMVVVSQVLWGNRVWNFKSGGSPRMKPVYRKLLIGSMIVASLSAGQFLTHNFQMGWNNDNTLAFSNTLYALKISMSLIKYLPQVKHNYERKTLRGFPIQSVVCDVMGSLCSLAQLVVQVTRDRTPLTTAVLSANFGRIGIAAVTLFFNFIYISQWLVYGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>C5DC90</accession>
    <name>C5DC90_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">KLTH0B01100p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0B01100g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551839.1">
        <property type="nucleotide sequence ID" value="XM_002551793.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DC90">
        <property type="interactions" value="828"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DC90"/>
    <dbReference type="GeneID" id="8290665"/>
    <dbReference type="KEGG" id="lth:KLTH0B01100g"/>
    <dbReference type="eggNOG" id="KOG0055">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_17_8_1"/>
    <dbReference type="InParanoid" id="C5DC90"/>
    <dbReference type="OMA" id="TFWACLT"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015421">
        <property type="term" value="F:ABC-type oligopeptide transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090374">
        <property type="term" value="P:oligopeptide export from mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18577">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18578">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000604">
        <property type="entry name" value="ABC transporter B family member 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394:SF15">
        <property type="entry name" value="ALPHA-FACTOR-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="76"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="152"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="177"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="258"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="730"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="852"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="877"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="955"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="993"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="26"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="359"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="732"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="1060"/>
            <end position="1295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR21401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1298" mass="143954" checksum="75FA5C7F594C5149" modified="2009-07-28" version="1">MRQLRNLQLSNVYANVHLKQDWSLLVVITVCSVAYGLVPAVTSILTGTVFNLLQQPAAREFGSVNALMHELALRSMAILATGIASIPLCWLSVSAWMSLGERQAFRLRRKMLQSYLDKSMAWYDANEQISGDFTQLNRCVEELRSSSAESSAIVFQNVVTVAALLGSALYYSWSLTLIIMANSPLVIGFAVFFSRKVEKYAKRENSETSSAADAMAWSLDAAKMVRLLGTQDRELARFEACVQKCRQHFNKMSLFSSLNYSVLRFLTLCMFVQGFWYGNSRINIGKLKPGDVVTCFSSCVLLASTLNSTLHQIIILQKGAVGLQKIMAFVNSRDASESQKNKVEISFRRLPLCWERASITFQNVHFAYPTRPDDFVLKGVSLDFPSGETTFVIGKSGSGKSTIGNLLLGFYGIGSGTINVNGQDVKEINQSELARNIMLVEQTTSLFTETLANNVTIGEAGVDDDEIRLKQACQMAMLGNIIRDLAKGLETTIGCSGLDLSGGEQQRVALARAYFRNAPILILDEALSAQDRIHKSLLMEAIRKWRADKTTIILTHELSDILDTDYVYVMSDGQIREHGYKSGLLSNPKSEFSNLHRFQACDDDFGDVATLGDGTSQIVDQKLEGVRTDVEELYSELGSPSTLRTCCVPESDYHPVTSHALRSSVDLLQSSIMMPHRTRRVAKTKERVDATELGPLPTVPDIEKGQTEPSLLPLRDIIKQFFINSRQKRLLAVGGTSALLAGVANPLFSYTFSKLLSGIASSNAYPATGRRSTKWSLIVLSVALLDSTFTFLKCFTLSKCGENWIRALRLRAFKNISNRELAWFNSEQNSTSNVSSLVINDLRDLRALASEFLAAVTTLVVVSFCGLIWALASGWKLSLVCISLIPAFILFTGAYGGLLQKCEANYKSAVASMEDQLYSAVRRIKTIRCLQVEERVIKEYEVLEGKAKLAGRKRALGTGFGVALSNALTLITQSILLYYGMKLVITGEYSSERMFQTLTLLLFTIMTCMSLIAQIPEISRGQRAATYLFRILDSETQFADANLEGESALTSRGCSNEALIFIKDLRFSYSFLNNPMVYRGLNLSVKAGETVALVGLSGSGKSTLWHLITRLYRVPSGSIWFDGLDVNEWEEWALRKRIAVVEQRPSLFRGSIFDNLTYGLSTSVDEREIRELLELVGMMDFVNSNPLGLQSFIDTDVLSGGQLQRLAIVRALLRKPQLLVLDECTSALDAQHGFIMSEFVRQGSQSTTTLVLTHSEQMMRACNRILTFANGNIIEDGSFERLYSQKGELFKILSSCSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCB5</accession>
    <name>C5DCB5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Translation machinery-associated protein 22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0B01694g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3 8">Interacts with the 40S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">The SUI1 domain may be involved in RNA binding.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the DENR family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551864.1">
        <property type="nucleotide sequence ID" value="XM_002551818.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCB5"/>
    <dbReference type="FunCoup" id="C5DCB5">
        <property type="interactions" value="1269"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCB5"/>
    <dbReference type="GeneID" id="8290692"/>
    <dbReference type="KEGG" id="lth:KLTH0B01694g"/>
    <dbReference type="eggNOG" id="KOG3239">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073511_0_1_1"/>
    <dbReference type="InParanoid" id="C5DCB5"/>
    <dbReference type="OMA" id="EVFEIDM"/>
    <dbReference type="OrthoDB" id="277199at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002188">
        <property type="term" value="P:translation reinitiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11607">
        <property type="entry name" value="DENR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.780.10:FF:000013">
        <property type="entry name" value="Translation machinery-associated protein 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.780.10">
        <property type="entry name" value="SUI1-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050318">
        <property type="entry name" value="DENR/SUI1_TIF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046447">
        <property type="entry name" value="DENR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005873">
        <property type="entry name" value="DENR_eukaryotes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048517">
        <property type="entry name" value="DENR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001950">
        <property type="entry name" value="SUI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036877">
        <property type="entry name" value="SUI1_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01159">
        <property type="entry name" value="DRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12789:SF0">
        <property type="entry name" value="DENSITY-REGULATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12789">
        <property type="entry name" value="DENSITY-REGULATED PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21023">
        <property type="entry name" value="DENR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01253">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55159">
        <property type="entry name" value="eIF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50296">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6 8" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="SUI1" evidence="10">
        <location>
            <begin position="96"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="177"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR21426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="197" mass="22295" checksum="3FC58840C24B8B7C" modified="2009-07-28" version="1">MSLRTVIYCGVCSFPPEFCEFTGKLKRCKVWLLENHPEKYQSLYGDAEVEDAAAKLAGSSIGEEREEKLEKDLLKLQSKQENREQRELAKKLSSKVIIKREARTKRKTMVAIAGLEVFEIDMKKLAKTFASKFATGCSVSKNAEKKEEIIIQGDVADEAETYIQKLLEEKGLKEVKVEQMDMKKKKKPTTTTPTPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCB8</accession>
    <name>C5DCB8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">GPI mannosyltransferase 1</fullName>
            <ecNumber evidence="13">2.4.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">GPI mannosyltransferase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0B01760g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12 13">Mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers the first alpha-1,4-mannose to GlcN-acyl-PI during GPI precursor assembly. Required for cell wall integrity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Endoplasmic reticulum membrane</location>
            <topology evidence="1 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the PIGM family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="13"/>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551867.1">
        <property type="nucleotide sequence ID" value="XM_002551821.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCB8"/>
    <dbReference type="FunCoup" id="C5DCB8">
        <property type="interactions" value="626"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCB8"/>
    <dbReference type="GeneID" id="8290695"/>
    <dbReference type="KEGG" id="lth:KLTH0B01760g"/>
    <dbReference type="eggNOG" id="KOG3893">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024220_3_1_1"/>
    <dbReference type="InParanoid" id="C5DCB8"/>
    <dbReference type="OMA" id="LINCWIL"/>
    <dbReference type="OrthoDB" id="1741594at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990529">
        <property type="term" value="C:glycosylphosphatidylinositol-mannosyltransferase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051751">
        <property type="term" value="F:alpha-1,4-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004376">
        <property type="term" value="F:GPI mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007704">
        <property type="entry name" value="PIG-M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12886:SF0">
        <property type="entry name" value="GPI MANNOSYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12886">
        <property type="entry name" value="PIG-M MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05007">
        <property type="entry name" value="Mannosyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 13" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5 13" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="76"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="152"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="188"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="253"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="305"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="330"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="354"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU365064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR21429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="394" mass="45876" checksum="E1151031146FB09F" modified="2009-07-28" version="1">MDTATVSLFLGALALRLGFFFYGIYQDKYFEVKYTDVDYFVFNDAASYVFHGLSPYLRDTYRYTPLLSWLLVPNHYLQWIHFGKMMFVLFDLLTGLLILNLLEAQSKRKKLILSSLWLLNFMVITISTRGNAESVLCFLILLSLYFLKARQFVLSGLFLGFAIHFKIYPIIYSIPIAVYVYRQRQGGLFNVFKVGVSAILALAGTSYWMYQIYGMEFLDNAYFYHLSRTDHRHNFSVFHMLLYFESALPTDSFWAKLAFLPQAAITLGVVPLLLRDPSFNSLLSLLFIQTFAFVTYNKVCTSQYFIWYLIFLPFYLARTSITLKKGLTMALIWVGTQGAWLFFGYLLEFKGKNVFYPGLFLASLSFFLGNVWILGQFIDDFKVKTELSEEKKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCN1</accession>
    <name>C5DCN1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0B04444p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0B04444g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551980.1">
        <property type="nucleotide sequence ID" value="XM_002551934.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCN1"/>
    <dbReference type="FunCoup" id="C5DCN1">
        <property type="interactions" value="41"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCN1"/>
    <dbReference type="GeneID" id="8290817"/>
    <dbReference type="KEGG" id="lth:KLTH0B04444g"/>
    <dbReference type="eggNOG" id="KOG3028">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069449_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCN1"/>
    <dbReference type="OMA" id="PMWYNTP"/>
    <dbReference type="OrthoDB" id="5835136at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001401">
        <property type="term" value="C:SAM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019564">
        <property type="entry name" value="Sam37/metaxin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031317">
        <property type="entry name" value="Tom37_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10568">
        <property type="entry name" value="Tom37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11801">
        <property type="entry name" value="Tom37_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mitochondrial outer membrane transport complex Sam37/metaxin N-terminal" evidence="1">
        <location>
            <begin position="21"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Tom37 C-terminal" evidence="2">
        <location>
            <begin position="156"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR21542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="295" mass="33832" checksum="F074BCC184DF480C" modified="2009-07-28" version="1">MKATVHLWGLNGKPSIVSPESVALFWLLNDTQNDRNVTIVFSNNTDLSPNQELPLLIDGDKKLYGYANIARHFSAEETALEMALLQFTQDHICVLTQYQLYLNKNNYDSFTRRVFAYLLEWPLWYNTPLKYRALARKRCETLGYFGHDDDPEQVEQPNAELDGLVQSKAFKLTNASKARGEELLKSARYNLQYMSRLNRHVVSWREARARLDKDSTAADFLLWANLFVQMELPDGQLVKEQLQTALGPEDYESIIKRLNDCSKSTSTLELRGPFFAEKGNVVMSAYHALQRLVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCV9</accession>
    <name>C5DCV9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0B06204g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552058.1">
        <property type="nucleotide sequence ID" value="XM_002552012.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCV9"/>
    <dbReference type="FunCoup" id="C5DCV9">
        <property type="interactions" value="730"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCV9"/>
    <dbReference type="GeneID" id="8290897"/>
    <dbReference type="KEGG" id="lth:KLTH0B06204g"/>
    <dbReference type="eggNOG" id="KOG1165">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019279_1_0_1"/>
    <dbReference type="InParanoid" id="C5DCV9"/>
    <dbReference type="OMA" id="DWMHLNG"/>
    <dbReference type="OrthoDB" id="5800476at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14127">
        <property type="entry name" value="STKc_CK1_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000968">
        <property type="entry name" value="Casein kinase I, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000538">
        <property type="entry name" value="Putative Casein kinase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="32"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="331"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="408"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="363"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="422"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="442"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR21620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="498" mass="57167" checksum="8E95BD3584F9441F" modified="2009-07-28" version="1">MNGASASASQHVLGSSASSTARDDSTIVGLHYRIGKKIGEGSFGVLFEGTNMINGVPVAIKFEPRKSEAPQLKDEYRTYKILAGTPGIPQAYYFGQEGLHNILVIDLLGPSLEDLFDWCGRRFSVKTVVQVAVQMITLIEDLHTHDLIYRDIKPDNFLIGRPGTPDENKVHLIDFGMAKQYRDPKTKQHIPYREKKSLSGTARYMSINTHLGREQSRRDDMEALGHVFFYFLRGQLPWQGLKAPNNKQKYEKIGEKKRVTNVYDLAQGLPVQFGRYLEVVRSLAFEETPDYEGYRKLFLSILDDMDNQADGEYDWMKLNGGRGWDLNINKKPNLHGYGHPNPPNDKNRRHRNKYAQGQGMDPQQQLRYQQQQLLHQQQQQQQQQQQQQQQQQQQQQQQRQQQLQSQQQISQQQRLDPTSYEAYQQQTQQKYAQQQQKLQQQSRVHSYNQHAPQQQPNLYGAQQPPTVKQHAQPPNKVAATDGKSVSSGKGFFSKLGCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>C5DD65</accession>
    <name>C5DD65_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 14">6-phosphogluconate dehydrogenase, decarboxylating</fullName>
            <ecNumber evidence="10 14">1.1.1.44</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">KLTH0B08668g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
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        <text evidence="1 10">Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO(2), with concomitant reduction of NADP to NADPH.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10 14">
            <text>6-phospho-D-gluconate + NADP(+) = D-ribulose 5-phosphate + CO2 + NADPH</text>
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            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58759"/>
            <dbReference type="EC" id="1.1.1.44"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 10 14">Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 3/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 10">Homodimer.</text>
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    <comment type="similarity">
        <text evidence="3 10 14">Belongs to the 6-phosphogluconate dehydrogenase family.</text>
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        <location>
            <begin position="12"/>
            <end position="17"/>
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            <name>NADP(+)</name>
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            <end position="37"/>
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            <end position="79"/>
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        <source>
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            <dbReference type="Proteomes" id="UP000002036"/>
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</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDD8</accession>
    <name>C5DDD8_LACTC</name>
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        <submittedName>
            <fullName evidence="3">KLTH0B10472p</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="ordered locus">KLTH0B10472g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
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        <property type="entry name" value="AS_sf"/>
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        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR42678:SF37">
        <property type="entry name" value="AMIDASE C869.01-RELATED"/>
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    <proteinExistence type="predicted"/>
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        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950423" evidence="1">
        <location>
            <begin position="22"/>
            <end position="583"/>
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    <feature type="domain" description="Amidase" evidence="2">
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            <begin position="86"/>
            <end position="421"/>
        </location>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAR21799.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="583" mass="64361" checksum="BCB89C4094204E2D" modified="2009-07-28" version="1">MKIQSVCVLFLVLCLVEPSSLNQSRRKYASKSQTLFQTANTTTHLSSQIESDGLLLINSRSNVSLGYATIDQLQDYLSNGSLSSQDLVRYYLQRFQQLNPHVKGILQLNPDALKIAKERDEERTQGLVRGPLHGIPFLVKDNYATKDKMETTCGSWSLLGSVVPRDAFVVAKLRDAGAVLLGHTTLSEWADMRSSCYSEGYSARGGQARCPFDLSVSPGGSSSGSAVAVAADMVTFSLGTETDGSIIDPAMKNGIVGFKPTVGLTSRAGVVPESEHQDSTGPMARTVRDAVYAFQYMWGVDERDVYTLNQTGHVPQDGDYVKFLSDKESLKGARFGIPWQKLWTLAEENEISRLAEVIKMIESAGATIYNNTNFKNLDVVSSSGWDWNMGPANESEFTVVKVDFFNNIESYLSELENSDMRSLKDIVEYNNRNYETEGGTENTHHAFQSGQDSFMESLSWGGVQNETYWQAVEFVQKTSRGEGIDYALNYTDPETGENFNLDGLLVPSGLPITHQQAAKAGYPMITLPVASKESNGRPFGLGIIQSAWEEPKLIKYGSAIEDLLNYQLKPWSNDNLDKNISTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDN5</accession>
    <name>C5DDN5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Replication factor C subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0C02442g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the activator 1 small subunits family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552334.1">
        <property type="nucleotide sequence ID" value="XM_002552288.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDN5"/>
    <dbReference type="FunCoup" id="C5DDN5">
        <property type="interactions" value="668"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDN5"/>
    <dbReference type="GeneID" id="8291194"/>
    <dbReference type="KEGG" id="lth:KLTH0C02442g"/>
    <dbReference type="eggNOG" id="KOG0989">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042324_1_0_1"/>
    <dbReference type="InParanoid" id="C5DDN5"/>
    <dbReference type="OMA" id="GCQSGSF"/>
    <dbReference type="OrthoDB" id="4199794at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005663">
        <property type="term" value="C:DNA replication factor C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031391">
        <property type="term" value="C:Elg1 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18140">
        <property type="entry name" value="HLD_clamp_RFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.272.10:FF:000011">
        <property type="entry name" value="Replication factor C subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000237">
        <property type="entry name" value="replication factor C subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050238">
        <property type="entry name" value="DNA_Rep/Repair_Clamp_Loader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013748">
        <property type="entry name" value="Rep_factorC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047854">
        <property type="entry name" value="RFC_lid"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001679">
        <property type="entry name" value="PRK00440.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669">
        <property type="entry name" value="REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669:SF20">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21960">
        <property type="entry name" value="RCF1-5-like_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08542">
        <property type="entry name" value="Rep_fac_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="9">
        <location>
            <begin position="56"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR21896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="356" mass="40160" checksum="A50282DE9EF56195" modified="2009-07-28" version="1">MSSEGFFAKRQKLSQPENHGDDSKPWVEKYRPKKLEDVTAQDHAVNVLKKTLQSANLPHMLFYGPPGTGKTSTILALTKELYGPALMKSRVLELNASDERGIAIVRDKIKSFARLTVSKPSQNDREKYPCPPYKIIILDEADSMTADAQSALRRTMENYSNVTRFCLICNYVTRIIDPLASRCSKFRFKPLDSSNALSRVQYVAKEERLQYDEHVLEKILDVSQGDLRRAIMLLQSTSKIVKHLDPPQITALTVDELAGTVPTELLNELVAKIATANLETIKINVREMIKSGWSGASVVNQLHHYYINSDQYSTAFKNKASWLIFDADSKLTNGSNEHIQLLNLAMKLADLYRKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDS0</accession>
    <name>C5DDS0_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Guanosine-diphosphatase</fullName>
            <ecNumber evidence="11">3.6.1.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">KLTH0C03300g</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">After transfer of sugars to endogenous macromolecular acceptors, the enzyme converts nucleoside diphosphates to nucleoside monophosphates which in turn exit the Golgi lumen in a coupled antiporter reaction, allowing entry of additional nucleotide sugar from the cytosol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>GDP + H2O = GMP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22156"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.1.42"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the GDA1/CD39 NTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.42" evidence="11"/>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552369.1">
        <property type="nucleotide sequence ID" value="XM_002552323.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDS0"/>
    <dbReference type="FunCoup" id="C5DDS0">
        <property type="interactions" value="391"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDS0"/>
    <dbReference type="GeneID" id="8291233"/>
    <dbReference type="KEGG" id="lth:KLTH0C03300g"/>
    <dbReference type="eggNOG" id="KOG1385">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010246_4_1_1"/>
    <dbReference type="InParanoid" id="C5DDS0"/>
    <dbReference type="OMA" id="WTCRIKE"/>
    <dbReference type="OrthoDB" id="6372431at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004382">
        <property type="term" value="F:GDP phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017111">
        <property type="term" value="F:ribonucleoside triphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045134">
        <property type="term" value="F:UDP phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009134">
        <property type="term" value="P:nucleoside diphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd24040">
        <property type="entry name" value="ASKHA_NBD_GDA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.150:FF:000009">
        <property type="entry name" value="Guanosine-diphosphatase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.150">
        <property type="entry name" value="Exopolyphosphatase. Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000407">
        <property type="entry name" value="GDA1_CD39_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11782">
        <property type="entry name" value="ADENOSINE/GUANOSINE DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11782:SF83">
        <property type="entry name" value="GUANOSINE-DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01150">
        <property type="entry name" value="GDA1_CD39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01238">
        <property type="entry name" value="GDA1_CD39_NTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002948684" description="Guanosine-diphosphatase" evidence="17">
        <location>
            <begin position="32"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="224"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="255"/>
            <end position="259"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00050779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00067799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR600407-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR600407-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAR21931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="523" mass="57769" checksum="C25BAC58A1A2440F" modified="2009-07-28" version="1">MAGLGSLVRNYRFLIAMLSAVMMILLLRSSASTAGISEQVSTKIGQTDIDDVYKTQPIKQEPGYLEDESIKKADKKLEDAVKQSQQNPEYSTGTKCTEEHQYVVMIDAGSTGSRVHIYEFDMCTKPPTLLHETFEMLKPGLSSYGSDPAGAAQSLDKLLDLAVEVIPEKRRKCSPVAVKATAGLRKLGDEKSAKILEAVRAHLEKDYPFPVVENGAVVMTGDEEGVYAWITANYLLGNIAGREKVPTAATFDLGGGSTQIVFEPAFPPNEKMIEGEHKYPLQFGDEKYTLYQFSHLGYGLMEGRNKVNSLLVENAIKDKKISKDDKVAKLFSPCLPPGAIAEDVEVEMPDKTSKTVTFEGPVTPAGPQCRALAEKVLHKDAKCSEPPCSFNGVHQPSLVRTFKESNDLYVFSYFYDKTQPLGLPSSFTLQELADLARMVCNGESVWESVFSGIPGSIEKLEKEPQWCLDLSFQVALLHTGYDIPLYRELKTAEKISDYELGWCLGASLPLLEGKDWKCKIEQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDS9</accession>
    <name>C5DDS9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DNA replication licensing factor MCM3</fullName>
            <ecNumber evidence="12">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0C03520g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 17">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the MCM2-7 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the MCM family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="12"/>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552378.1">
        <property type="nucleotide sequence ID" value="XM_002552332.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDS9"/>
    <dbReference type="FunCoup" id="C5DDS9">
        <property type="interactions" value="1228"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDS9"/>
    <dbReference type="GeneID" id="8291242"/>
    <dbReference type="KEGG" id="lth:KLTH0C03520g"/>
    <dbReference type="eggNOG" id="KOG0479">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000995_6_1_1"/>
    <dbReference type="InParanoid" id="C5DDS9"/>
    <dbReference type="OMA" id="GRFHYRD"/>
    <dbReference type="OrthoDB" id="1882346at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071162">
        <property type="term" value="C:CMG complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005656">
        <property type="term" value="C:nuclear pre-replicative complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902975">
        <property type="term" value="P:mitotic DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006267">
        <property type="term" value="P:pre-replicative complex assembly involved in nuclear cell cycle DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006279">
        <property type="term" value="P:premeiotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17754">
        <property type="entry name" value="MCM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.10:FF:000008">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1640.10">
        <property type="entry name" value="mini-chromosome maintenance (MCM) complex, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008046">
        <property type="entry name" value="Mcm3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018525">
        <property type="entry name" value="MCM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027925">
        <property type="entry name" value="MCM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056575">
        <property type="entry name" value="WH_MCM3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054007">
        <property type="entry name" value="WHD_MCM3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF46">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14551">
        <property type="entry name" value="MCM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22207">
        <property type="entry name" value="WHD_MCM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23191">
        <property type="entry name" value="WHD_MCM3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01659">
        <property type="entry name" value="MCMPROTEIN3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00847">
        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 12" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 12" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="14">
        <location>
            <begin position="382"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="758"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="861"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="791"/>
            <end position="808"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU368061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR21940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="994" mass="110440" checksum="3CE59BC91316C96C" modified="2009-07-28" version="1">MASVTNARLPVRVLKLETSIFSHRSTPQLIVETERVPLVFLLPRNPTFESMESINDTPDAVFGDRVRRFQEFLDTFSSYKDAIRFVQLHNLSDQAFSQDPTKLKSEVEGKVSQRITVSLDDLREFDRSFWAGILNMPAQYVPAAERALSETAMTLEESPAGNLGNLLAQQWKLSFRGSFGAHALSPRTLNSHHLNKLISVEGIVTKVSLVRPKLLRSVHYAEKTGRFHYRDYRDATTTLTTQIPTPAIYPTEDPEGNRLTTEYGYSTYVDHQRITVQEMPEKAPPGQLPRSIDVIMDEDLVDKAKPGDRINIVGIYKSLGGGGLSAGSGGKDQTGALSGFRTVILANTAYPLHARSTGVAARQALSDSDIRNINKLSKKDDIFDLLSQSLAPSIYGHEQIKKAILLMLMGGVEKNLENGSHLRGDINLLMVGDPSTAKSQLLRFVLNTASLAIATTGRGSSGVGLTAAVTMDRETGERRLEAGAMVLADRGIVCIDEFDKMSDVDRVAIHEVMEQQTVTIAKAGIHTTLNARCSVIAAANPVFGQYDLNKDPHYNIALPDSLLSRFDLLFVVTDDINENTDRAISEHVLRTHRYLPPGYLEGEPIREAINLSLSVGEDTEANNEEEDDDDDEGRVFEKFNPLLHAGAKLARNKGNRQGTELPQIVCIPFLRKYVQYAKERVVPVLTQGAVSLIVKAYTELRNDQNTKKSPITARTLETLIRLSSAHAKVRLSKTVNRADARVASQLLRFALLGEDDLGAEFDTDDNNERSPTKSPRKRQRVRNATAAARDLQSSPTRAPNLSTSTPTRTDLRRRLYSDDESEQEIQASGIQEDDEFMQQDESDMTALPADQEEDLQQRLQRGLRVSPRRQESQRASTPVTEDGRHVLLPSENFRSRPILAADQSIDDQDLVSAEEMAPGAISTSRLSLFSGIIARLMQTDLFEDESYPVASLLDKINEELSEEDKFSAPEYLAGLKVMSDRNNLMVAEEKVWRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDT9</accession>
    <name>C5DDT9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">N-acetylglucosaminylphosphatidylinositol deacetylase</fullName>
            <ecNumber evidence="2">3.5.1.89</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0C03740g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PIGL family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.89" evidence="2"/>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552388.1">
        <property type="nucleotide sequence ID" value="XM_002552342.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDT9"/>
    <dbReference type="FunCoup" id="C5DDT9">
        <property type="interactions" value="564"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDT9"/>
    <dbReference type="GeneID" id="8291252"/>
    <dbReference type="KEGG" id="lth:KLTH0C03740g"/>
    <dbReference type="eggNOG" id="KOG3332">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034979_0_0_1"/>
    <dbReference type="InParanoid" id="C5DDT9"/>
    <dbReference type="OMA" id="YVLESVN"/>
    <dbReference type="OrthoDB" id="440160at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000225">
        <property type="term" value="F:N-acetylglucosaminylphosphatidylinositol deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10320">
        <property type="entry name" value="LmbE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003737">
        <property type="entry name" value="GlcNAc_PI_deacetylase-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024078">
        <property type="entry name" value="LmbE-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12993:SF11">
        <property type="entry name" value="N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL DE-N-ACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12993">
        <property type="entry name" value="N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL DE-N-ACETYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02585">
        <property type="entry name" value="PIG-L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102588">
        <property type="entry name" value="LmbE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950334" description="N-acetylglucosaminylphosphatidylinositol deacetylase" evidence="3">
        <location>
            <begin position="23"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR21950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="254" mass="29188" checksum="F360785BA6DE6B23" modified="2009-07-28" version="1">MRILKVVCKLLAVLCILYFASSARIKASNEQAFTTLFSVLNPTSINLVIAHPDDEVMFFAPTLLQLDHYFDTDIPVSVFSLTDGGADGLGKLREEELRDSVHLLFRRRKAEVTVLNFEDGMKVEWDLQKTSEALRKQVVGDQPLFLTFDDHGVSSHINHISCYETVRNLRAAHPESRSFSLTSKRSIFQKYTAFVPSLITVLRGSRQAVFMSTFKQYLLALSVMMNAHTSQMVWFRYGWWFFSCYVFANEVREF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2010-10-05" modified="2025-06-18" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DE09</accession>
    <name>AIM24_LACTC</name>
    <protein>
        <recommendedName>
            <fullName>Altered inheritance of mitochondria protein 24, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">aim24</name>
        <name type="ordered locus">KLTH0C05368g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the AIM24 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552458.1">
        <property type="nucleotide sequence ID" value="XM_002552412.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DE09"/>
    <dbReference type="FunCoup" id="C5DE09">
        <property type="interactions" value="18"/>
    </dbReference>
    <dbReference type="GeneID" id="8291325"/>
    <dbReference type="KEGG" id="lth:KLTH0C05368g"/>
    <dbReference type="eggNOG" id="ENOG502RXC5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057912_0_0_1"/>
    <dbReference type="InParanoid" id="C5DE09"/>
    <dbReference type="OMA" id="NGPYDLQ"/>
    <dbReference type="OrthoDB" id="5295771at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007007">
        <property type="term" value="P:inner mitochondrial membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.160.10">
        <property type="entry name" value="Mitochondrial biogenesis AIM24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002838">
        <property type="entry name" value="AIM24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036983">
        <property type="entry name" value="AIM24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016031">
        <property type="entry name" value="Trp_RNA-bd_attenuator-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36959">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 24, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36959:SF2">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 24, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01987">
        <property type="entry name" value="AIM24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51219">
        <property type="entry name" value="TRAP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0496">Mitochondrion</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0809">Transit peptide</keyword>
    <feature type="transit peptide" description="Mitochondrion" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000399581" description="Altered inheritance of mitochondria protein 24, mitochondrial">
        <location>
            <begin position="26"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="343"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="343"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="402" mass="43285" checksum="B73942A2C907FF47" modified="2009-07-28" version="1" precursor="true">MKAAALRLGHASVAKRSISLVRPVAATVVPAQMAQKPDAEPIFGQEDNSDIQDAQFRVLGQPPTLCSVHVPASVSLHVRRGCLVSLHGVGEQNAVAISQEWIGLAWNLAKHGSWRAALFHQLVAPRAFSALVAPNVNGGKIASWFGVSAQQFRTLCLLDLDGTQDWCVLGKHSLIAYEGNTSLRIQRSSVWPSWLHFNRGDSALPSTYQLIQGRGNALLSGAGSVYTIELPNENEELILKGEHLLAISGRTPRDIAEAVTEYSFPAVSVPAIPETAVSKGRTPMPTSSLSAFSTALASYSRNAVSWLRHAYTSYINGTTKYLRIRGPRFLLVQSAHNAFMPATSPSAVQPPSSPVSVKAPASTPNSKDYLSYVTVGKDASVSFQSTPDFNKKVAEIEALKHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DE40</accession>
    <name>C5DE40_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">L17A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">L17a</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">YL32</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0C06072g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the universal ribosomal protein uL14 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552489.1">
        <property type="nucleotide sequence ID" value="XM_002552443.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DE40"/>
    <dbReference type="FunCoup" id="C5DE40">
        <property type="interactions" value="1160"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DE40"/>
    <dbReference type="GeneID" id="8291357"/>
    <dbReference type="KEGG" id="lth:KLTH0C06072g"/>
    <dbReference type="eggNOG" id="KOG0901">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095071_3_0_1"/>
    <dbReference type="InParanoid" id="C5DE40"/>
    <dbReference type="OMA" id="IRQSKPW"/>
    <dbReference type="OrthoDB" id="407959at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00337">
        <property type="entry name" value="Ribosomal_uL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.150.20:FF:000003">
        <property type="entry name" value="60S ribosomal protein L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.150.20">
        <property type="entry name" value="Ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01367">
        <property type="entry name" value="Ribosomal_uL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000218">
        <property type="entry name" value="Ribosomal_uL14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019972">
        <property type="entry name" value="Ribosomal_uL14_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036853">
        <property type="entry name" value="Ribosomal_uL14_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11761">
        <property type="entry name" value="50S/60S RIBOSOMAL PROTEIN L14/L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11761:SF8">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00238">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01374">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50193">
        <property type="entry name" value="Ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00049">
        <property type="entry name" value="RIBOSOMAL_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 7" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00079088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00079110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00082613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR22051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="137" mass="14519" checksum="615B491651F51B57" modified="2009-07-28" version="1">MSGNGAQGTKFRISLGLPTGAIMNCADNTGARNLYIMAVKGSGSRLNRLPAASLGDMVMATVKKGKPELRKKVMPAIVVRQSKPWRRKDGVFLYFEDNAGVIANPKGEMKGSAITGPVGKECADLWPRIASNSGVVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DED9</accession>
    <name>C5DED9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0C08404p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0C08404g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peroxisomal membrane protein PXMP2/4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552588.1">
        <property type="nucleotide sequence ID" value="XM_002552542.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DED9"/>
    <dbReference type="STRING" id="559295.C5DED9"/>
    <dbReference type="GeneID" id="8291462"/>
    <dbReference type="KEGG" id="lth:KLTH0C08404g"/>
    <dbReference type="eggNOG" id="KOG1944">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066033_2_0_1"/>
    <dbReference type="InParanoid" id="C5DED9"/>
    <dbReference type="OMA" id="HYGYQWL"/>
    <dbReference type="OrthoDB" id="860at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007248">
        <property type="entry name" value="Mpv17_PMP22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266:SF93">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN 25D9-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="121"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="160"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR22150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="263" mass="29584" checksum="008EFFB9664CD2C3" modified="2009-07-28" version="1">MLFFRDKFIVIAYVRYIKNRIYINALTKLKTTAHGLRNMSVASMSVQTRQISIAARYNVLLTKYPLLTKMVTGAVLSALSELVSQFTTASKTQDKDEKQLSILQKLASALQKPRYRKLLSMFLYGGLVNAPINHFCYGWITRFTSRNVAPKWKRMAQLCGSWFIVSPVQVFFLVIALTLVNLDQGASKLSQKLDAVKSSLRSKYGPMLSSSVISATAFVSIAQQFIAPEKWSVFFSFAYAALNTGQNIYLKRGAKDSSKERAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEF1</accession>
    <name>C5DEF1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">KLTH0C08668p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0C08668g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SF3A2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552600.1">
        <property type="nucleotide sequence ID" value="XM_002552554.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEF1"/>
    <dbReference type="FunCoup" id="C5DEF1">
        <property type="interactions" value="473"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEF1"/>
    <dbReference type="GeneID" id="8291474"/>
    <dbReference type="KEGG" id="lth:KLTH0C08668g"/>
    <dbReference type="eggNOG" id="KOG0227">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087074_0_0_1"/>
    <dbReference type="InParanoid" id="C5DEF1"/>
    <dbReference type="OMA" id="EFWIQIM"/>
    <dbReference type="OrthoDB" id="10250970at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000245">
        <property type="term" value="P:spliceosomal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2690">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052092">
        <property type="entry name" value="SF3A2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031781">
        <property type="entry name" value="SF3A2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23205">
        <property type="entry name" value="SPLICING FACTOR 3A SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23205:SF0">
        <property type="entry name" value="SPLICING FACTOR 3A SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16835">
        <property type="entry name" value="SF3A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="U1-type" evidence="10">
        <location>
            <begin position="66"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR22162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="252" mass="28731" checksum="B85218C916035B68" modified="2009-07-28" version="1">MDYQNRAGSKKGSGGIASSSQENLHRRKQVEDLLKDGEEVPYSFQGISKEDEELSKKNPYIYKNHAGRLVCKLCNTIHMSWTSVERHLEGKKHGLNLIRRGGTTSSQQQDAVSPEELKFKKTVEQLRSQIKDNGIVPNYQVAKVRDPTTNSRGIAMRVIYTNPEPSEDGGDRPYVRIMSSLEFTREESDKKYLIIAFEPFKAIAIEIPNEEILTNSAAFNTSGAKSVDDFNSKFTYWDEDNQTLYVQFFFKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEH0</accession>
    <name>C5DEH0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0C09130p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0C09130g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552619.1">
        <property type="nucleotide sequence ID" value="XM_002552573.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEH0"/>
    <dbReference type="FunCoup" id="C5DEH0">
        <property type="interactions" value="647"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEH0"/>
    <dbReference type="GeneID" id="8291495"/>
    <dbReference type="KEGG" id="lth:KLTH0C09130g"/>
    <dbReference type="eggNOG" id="KOG2890">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043563_1_0_1"/>
    <dbReference type="InParanoid" id="C5DEH0"/>
    <dbReference type="OMA" id="EIHFWEV"/>
    <dbReference type="OrthoDB" id="73612at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013861">
        <property type="entry name" value="TMEM115/Pdh1/Rbl19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13377">
        <property type="entry name" value="PLACENTAL PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13377:SF3">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08551">
        <property type="entry name" value="DUF1751"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01160">
        <property type="entry name" value="DUF1751"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="120"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="156"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="190"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="215"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR22181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="332" mass="37941" checksum="98832EE7BCDFFCFF" modified="2009-07-28" version="1">MHYRSKIFEINVPPSVLNARDLPNATALLTAAYIVFTLSLWWVKWSYPSLEANADATRAPVFSPILQLVPAQTLYDPWSLVLSNLVDVKWWKIGVDLLNLVVGGSFIERNWNSSWELVRFLLVIGSVSNLVVVLVTILSSAVFPKVRLDTPLDGNYTMLVGFCIVYKQLIPETTIFHIRNLPLVSKNFRFKLLPIFVLVVSTVTQLMFLRHFSELLSIWVTFLCSWVYLRFFQVLPPAITGAASRSLVGDASDTFQLIYFFPDIIKPFLSPIFNMCYWVFCVKLRIREPFNEDDVDTGNSLAEQRGAKVITSQVEERRRKLALQVLQERGTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEH5</accession>
    <name>C5DEH5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0C09284p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0C09284g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AHA1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552624.1">
        <property type="nucleotide sequence ID" value="XM_002552578.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEH5"/>
    <dbReference type="FunCoup" id="C5DEH5">
        <property type="interactions" value="104"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEH5"/>
    <dbReference type="GeneID" id="8291502"/>
    <dbReference type="KEGG" id="lth:KLTH0C09284g"/>
    <dbReference type="eggNOG" id="KOG2936">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_132818_0_0_1"/>
    <dbReference type="InParanoid" id="C5DEH5"/>
    <dbReference type="OMA" id="EFMHDED"/>
    <dbReference type="OrthoDB" id="567237at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.10.20">
        <property type="entry name" value="Activator of Hsp90 ATPase Aha1, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036338">
        <property type="entry name" value="Aha1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015310">
        <property type="entry name" value="AHSA1-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13009">
        <property type="entry name" value="HEAT SHOCK PROTEIN 90 HSP90 CO-CHAPERONE AHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13009:SF15">
        <property type="entry name" value="HSP90 CO-CHAPERONE HCH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09229">
        <property type="entry name" value="Aha1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01000">
        <property type="entry name" value="Aha1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103111">
        <property type="entry name" value="Activator of Hsp90 ATPase, Aha1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Activator of Hsp90 ATPase AHSA1-like N-terminal" evidence="2">
        <location>
            <begin position="13"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR22186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="145" mass="16511" checksum="A35FAF40F8A05DC4" modified="2009-07-28" version="1">MVVLNPNNWHWVDKNTLPWTQTYMQELKISCDAKDGATTVRVTRVNSVSGDSHVSQRKGKVICYFDLDLSFATEVVEAESGNSVCEGKIVVPELVHDETDFEIRPEGFGDHHALVRDEFVPLLREALCKYQQDLIDHHSRDVQQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEN6</accession>
    <name>C5DEN6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0C10736p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0C10736g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RNA 3'-terminal cyclase family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552685.1">
        <property type="nucleotide sequence ID" value="XM_002552639.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEN6"/>
    <dbReference type="FunCoup" id="C5DEN6">
        <property type="interactions" value="854"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEN6"/>
    <dbReference type="GeneID" id="8291567"/>
    <dbReference type="KEGG" id="lth:KLTH0C10736g"/>
    <dbReference type="eggNOG" id="KOG3980">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027882_1_0_1"/>
    <dbReference type="InParanoid" id="C5DEN6"/>
    <dbReference type="OMA" id="YTDQNKG"/>
    <dbReference type="OrthoDB" id="1911237at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000479">
        <property type="term" value="P:endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00875">
        <property type="entry name" value="RNA_Cyclase_Class_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.20:FF:000004">
        <property type="entry name" value="18S rRNA biogenesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.20">
        <property type="entry name" value="RNA 3'-terminal phosphate cyclase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.20">
        <property type="entry name" value="RNA 3'-terminal phosphate cyclase, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013791">
        <property type="entry name" value="RNA3'-term_phos_cycl_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023797">
        <property type="entry name" value="RNA3'_phos_cyclase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037136">
        <property type="entry name" value="RNA3'_phos_cyclase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000228">
        <property type="entry name" value="RNA3'_term_phos_cyc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016443">
        <property type="entry name" value="RNA3'_term_phos_cyc_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020719">
        <property type="entry name" value="RNA3'_term_phos_cycl-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036553">
        <property type="entry name" value="RPTC_insert"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03400">
        <property type="entry name" value="18S_RNA_Rcl1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11096">
        <property type="entry name" value="RNA 3' TERMINAL PHOSPHATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11096:SF1">
        <property type="entry name" value="RNA 3'-TERMINAL PHOSPHATE CYCLASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01137">
        <property type="entry name" value="RTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05189">
        <property type="entry name" value="RTC_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="EPT/RTPC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01287">
        <property type="entry name" value="RTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="RNA 3'-terminal phosphate cyclase" evidence="5">
        <location>
            <begin position="9"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="RNA 3'-terminal phosphate cyclase insert" evidence="6">
        <location>
            <begin position="182"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR22247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="364" mass="39923" checksum="7C9B15341E5D9907" modified="2009-07-28" version="1">MSSSRITSFQGSRNFRLLIVLATLSGKPIKIEKIRSDDLNPGLRDHEVSFLRLMEAVTNGSSIEISYTGTTIIYKPGIITGGSYTHNCPPSKPVGYYVEPMLYLAPFSKNKFSIIFRGVTASHNDAGVEAIKWGLLPVMEKFGVRECALHTLKRGSPPLGGGEVHLVVDSLIAQPVTMHALDTPQIASIRGVAYSTRVSPSMVNRMIEGARNVLRGLSCEVDITADVWRGENSGKSPGWGITLVAESKKGWRYFAENIGGAGDVPEDIGTQVAYELLEEISESAVVGRTQLPLAIVYMVIGKEDIGRLRITKSQVDERFIWLLRHIKKMFGTEVFLKPVDEPESDDIIVTIKGIGFTNTNKKTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>C5DES8</accession>
    <name>C5DES8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0C11858p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0C11858g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552727.1">
        <property type="nucleotide sequence ID" value="XM_002552681.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DES8"/>
    <dbReference type="STRING" id="559295.C5DES8"/>
    <dbReference type="GeneID" id="8291618"/>
    <dbReference type="KEGG" id="lth:KLTH0C11858g"/>
    <dbReference type="eggNOG" id="KOG0537">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0538">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020639_1_1_1"/>
    <dbReference type="InParanoid" id="C5DES8"/>
    <dbReference type="OMA" id="FFRPKIL"/>
    <dbReference type="OrthoDB" id="1925334at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000262">
        <property type="entry name" value="FMN-dep_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037396">
        <property type="entry name" value="FMN_HAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008259">
        <property type="entry name" value="FMN_hydac_DH_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578:SF148">
        <property type="entry name" value="L-LACTATE DEHYDROGENASE (CYTOCHROME)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578">
        <property type="entry name" value="S -2-HYDROXY-ACID OXIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01070">
        <property type="entry name" value="FMN_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00557">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51349">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0349">Heme</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="6">
        <location>
            <begin position="73"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="FMN hydroxy acid dehydrogenase" evidence="7">
        <location>
            <begin position="172"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR22289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="555" mass="62400" checksum="BD211F14F1122EBF" modified="2009-07-28" version="1">MLITKLARCKSLKQLRLCSFKDAKWPSTRKMSNLNPPIKATHPRRKEFRKASVLTGASASAALLAYSLLDFSSDGIEVSEVMQHNKPDDCWIVLNGEVYDVTSFLQMHPGGAARIMEVAGNDATRKFYSIHSDSTLEKMKEQLVYIGKLKGKFAETISEEDLRIRESRKNIPSLGLIFNLSDFEAVAKEVLPKSTYAYFATGSSDEFSIRENHYAYSRVFFKPMILQENEYDVDTSTEFLGSKVSLPVYISAFAGSKWAHPLAELNLQSAAYEADIMQMVPKQNSYSIEEFYENVPEDQKHWSQYHFDSREEFNEAGTLIKKLEAQPSVKALFLNVDLRDIGNREKDSRQRALDVESSKSLSAIVTSDKSYAKFTWKDIDQIMSSTKLPIGLKGVQRGEDVVLAAEKGVKAVVLSNHGGRQLDFSRPPLEVLAEAKQMLKERGLEDKIEIYLDGGIRRGSDVIKALCLGAKGVGLGRPFLYAMAGYGEEGVSHLLDILRNEMKNNMRLLGVDKVEDLNENLVDIGSLKFRNPRANDVLYDEAYQPLRFPPVRSAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5DET7</accession>
    <name>C5DET7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Isopentenyl-diphosphate Delta-isomerase</fullName>
            <ecNumber evidence="4">5.3.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Isopentenyl pyrophosphate isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Isopentenyl-diphosphate delta-isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">KLTH0D09658g</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>isopentenyl diphosphate = dimethylallyl diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23284"/>
            <dbReference type="ChEBI" id="CHEBI:57623"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="EC" id="5.3.3.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:23285"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from isopentenyl diphosphate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the IPP isomerase type 1 family.</text>
    </comment>
    <dbReference type="EC" id="5.3.3.2" evidence="4"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553130.1">
        <property type="nucleotide sequence ID" value="XM_002553084.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DET7"/>
    <dbReference type="FunCoup" id="C5DET7">
        <property type="interactions" value="808"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DET7"/>
    <dbReference type="GeneID" id="8295368"/>
    <dbReference type="KEGG" id="lth:KLTH0D09658g"/>
    <dbReference type="eggNOG" id="KOG0142">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060552_0_2_1"/>
    <dbReference type="InParanoid" id="C5DET7"/>
    <dbReference type="OMA" id="KAPFDNG"/>
    <dbReference type="OrthoDB" id="510307at2759"/>
    <dbReference type="UniPathway" id="UPA00059">
        <property type="reaction ID" value="UER00104"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004452">
        <property type="term" value="F:isopentenyl-diphosphate delta-isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050992">
        <property type="term" value="P:dimethylallyl diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009240">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006694">
        <property type="term" value="P:steroid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02885">
        <property type="entry name" value="NUDIX_IPP_Isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000012">
        <property type="entry name" value="Isopentenyl-diphosphate Delta-isomerase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011876">
        <property type="entry name" value="IsopentenylPP_isomerase_typ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02150">
        <property type="entry name" value="IPP_isom_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10885">
        <property type="entry name" value="ISOPENTENYL-DIPHOSPHATE DELTA-ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10885:SF0">
        <property type="entry name" value="ISOPENTENYL-DIPHOSPHATE DELTA-ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018427">
        <property type="entry name" value="Isopntndiph_ism"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="5" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0752">Steroid biosynthesis</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="16">
        <location>
            <begin position="90"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00071307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00072489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00083206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAR22692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="276" mass="31576" checksum="C419CDCFFCF4D2F7" modified="2009-07-28" version="1">MSSYSELVKGLDASHILSQFQDVIPLQKRPNTHSSSSSAEDNGDVVFEGHDEEQIRLMCENCIVVDWSDRAVGAGTKKLCHLMTNIELGLLHRAFSVFLFNEQGQLLLQQRATEKITFPDLWTNTCCSHPLCVDDELGLTGTLDDKVAGAQTAAVRKLEQELGIPMTETVAKTDFHFLNRIHYKAPSNGPWGEHEIDYIFFCQLRAGQSLTVEPNPNEVRDYKWVTAEQLKSMFQDPQFKFTPWFKLICESYLFQWWDQLSDLSQVENDSQIHRML</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEV0</accession>
    <name>C5DEV0_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Spindle pole component 29</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">SPC29</name>
        <name evidence="9" type="ordered locus">KLTH0D09966g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the spindle pole body (SPB) required for the proper execution of spindle pole body (SPB) duplication. Links the central plaque component SPC42 to the inner plaque component SPC110.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
            <location evidence="7">Cytoskeleton</location>
            <location evidence="7">Microtubule organizing center</location>
            <location evidence="7">Spindle pole body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the SPC29 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553143.1">
        <property type="nucleotide sequence ID" value="XM_002553097.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEV0"/>
    <dbReference type="FunCoup" id="C5DEV0">
        <property type="interactions" value="269"/>
    </dbReference>
    <dbReference type="GeneID" id="8295382"/>
    <dbReference type="KEGG" id="lth:KLTH0D09966g"/>
    <dbReference type="HOGENOM" id="CLU_1277817_0_0_1"/>
    <dbReference type="InParanoid" id="C5DEV0"/>
    <dbReference type="OMA" id="YLRHATN"/>
    <dbReference type="OrthoDB" id="4036034at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005823">
        <property type="term" value="C:central plaque of spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030474">
        <property type="term" value="P:spindle pole body duplication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031392">
        <property type="entry name" value="Spc29"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17082">
        <property type="entry name" value="Spc29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="38"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="38"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR22705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="216" mass="24842" checksum="A01BAD67FC4681D4" modified="2009-07-28" version="1">MSQPGLSAFLSNPETDDTLQNIRREYLASKKNLQNLLVTSPTRARTRPLQQIPTPPINGSKERADDDKLRQQLREGLQPDRFMNRPPQVPIPTAARSDIENLRSMLYSQQRVIDELTRGLDAQVIRNSALENRVSMLERYITRLESQGNSRSPEPQLVDEGISRRLRQQYTPLYTDDENTRVLLNSGAVDRPDRQISNFDDSTTKLLQIARDTSRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEW6</accession>
    <name>C5DEW6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0D10340p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0D10340g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uL18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553159.1">
        <property type="nucleotide sequence ID" value="XM_002553113.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEW6"/>
    <dbReference type="FunCoup" id="C5DEW6">
        <property type="interactions" value="1377"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEW6"/>
    <dbReference type="GeneID" id="8295398"/>
    <dbReference type="KEGG" id="lth:KLTH0D10340g"/>
    <dbReference type="eggNOG" id="KOG0875">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056222_1_0_1"/>
    <dbReference type="InParanoid" id="C5DEW6"/>
    <dbReference type="OMA" id="CQIASAH"/>
    <dbReference type="OrthoDB" id="1618453at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008097">
        <property type="term" value="F:5S rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00432">
        <property type="entry name" value="Ribosomal_L18_L5e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.100:FF:000002">
        <property type="entry name" value="60S ribosomal protein L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01337_A">
        <property type="entry name" value="Ribosomal_uL18_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005485">
        <property type="entry name" value="Rbsml_uL18_euk_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057268">
        <property type="entry name" value="Ribosomal_L18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025607">
        <property type="entry name" value="Ribosomal_uL18_C_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23410:SF12">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23410">
        <property type="entry name" value="RIBOSOMAL PROTEIN L5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14204">
        <property type="entry name" value="Ribosomal_L18_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17144">
        <property type="entry name" value="Ribosomal_L5e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00058">
        <property type="entry name" value="RIBOSOMALL5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL18 C-terminal eukaryotes" evidence="6">
        <location>
            <begin position="236"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF14204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR22721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="295" mass="33719" checksum="EB0630100EC4E4CC" modified="2009-07-28" version="1">MAFQKEIKSSAYHSRFQTPFRRRREGKTDYYQRKRLVAQHKAKYSSPKYRLVVRFTNKDIICQIVSSTITGDVVLVAAYSHELPRYGITHGLTNWAAAYATGLLVARRALQKLGLDETYPGVEEVEGEYQLTEAVEDGPRPFKVFLDIGLQRTTTGARVFGALKGASDGGLYVPHSESRFPGWDFEAEELDADMLRTYIFGGHVSQYMEELADDDEERFKELFKGYLADDIDADAVEDIYLEAHEKIREDPSFKPTEKKFTKEQYAAESKKYRQTKLSKEERRARVAAKIAALAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DF58</accession>
    <name>C5DF58_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein EFR3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0D12430g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the EFR3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553251.1">
        <property type="nucleotide sequence ID" value="XM_002553205.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DF58"/>
    <dbReference type="FunCoup" id="C5DF58">
        <property type="interactions" value="76"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DF58"/>
    <dbReference type="GeneID" id="8295491"/>
    <dbReference type="KEGG" id="lth:KLTH0D12430g"/>
    <dbReference type="eggNOG" id="KOG1877">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_371806_0_0_1"/>
    <dbReference type="InParanoid" id="C5DF58"/>
    <dbReference type="OMA" id="LYYVNSR"/>
    <dbReference type="OrthoDB" id="19232at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039786">
        <property type="entry name" value="EFR3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049150">
        <property type="entry name" value="EFR3_HEAT-like_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47766">
        <property type="entry name" value="PROTEIN EFR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47766:SF1">
        <property type="entry name" value="PROTEIN EFR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21072">
        <property type="entry name" value="EFR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="555"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="555"/>
            <end position="564"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR22813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="726" mass="81994" checksum="9A28A0F744F023B1" modified="2009-07-28" version="1">MRILFTPKHQKLVNQCYPTGRTPDKKPKSSETSYLLYYVNSRRTKLEKVSTYLAKKCVADLNHRRIGNITVTLELMNKIVNHCKENLNVFVKDFLQIMIKILSNANVNNDVGVLEKVEETFGSICRNLDGAMCNGDTEFIDMFEVFVNLYLEVVTDRLRDDILLLKGCSDISVVTNLAATPQASKLLSRAAELSLTKYQERNPQYRVKNLEASSDQPIAKRLTRTQTQVPGLDDLTNSDDEAVNALRSFFNTAETDKLTLAIKALLKVLLRIPNKSLLQFICNGIPVHLRYIVILIFIRQLDKDKEQSVIVLKLISSLLVSEVSIVGLSILDVMRKLLNTQLAAGATAETSNQCATTIGDLNRKTYYKDQTSDMFSEILLRLRDERNLKFHNILRRDIQELAASTYTPSLSLEMFLDLTAYVDRPLKLFSVVSESVSGSFSISKLFHFIDSLTSEGLQQEFIAAAFSKFKGIALLAGLKHYLEYDSKNNDPYYIYHEEAAKFLGISDYRNQAQYKRKSGERFSRDDLLNYYSDVETNKYSEKGRRIIASNGNNISTTDLVSHSNNNEETHNKESFALPLPSNNNYEYTPKFTVSSDVRSLKTLRYNSPKVKDLKAAELKGAPASKRNGLSRGSQSVKSHITNITFLLSELDDDCNEIKIQDPDDEEVVGLEKTAIARSESLKLLNNASKGAKRLSIPVRLQEDEDDFKDANEDVQITSTRGKLFAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DF74</accession>
    <name>C5DF74_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0D12782p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0D12782g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosducin family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553267.1">
        <property type="nucleotide sequence ID" value="XM_002553221.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DF74"/>
    <dbReference type="FunCoup" id="C5DF74">
        <property type="interactions" value="527"/>
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    <dbReference type="STRING" id="559295.C5DF74"/>
    <dbReference type="GeneID" id="8295507"/>
    <dbReference type="KEGG" id="lth:KLTH0D12782g"/>
    <dbReference type="eggNOG" id="KOG3170">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072604_1_0_1"/>
    <dbReference type="InParanoid" id="C5DF74"/>
    <dbReference type="OMA" id="FCEIRAN"/>
    <dbReference type="OrthoDB" id="45518at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02988">
        <property type="entry name" value="Phd_like_VIAF"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosducin-like protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051498">
        <property type="entry name" value="Phosducin-like_chap/apop_reg"/>
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    <dbReference type="InterPro" id="IPR024253">
        <property type="entry name" value="Phosducin_thioredoxin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45809">
        <property type="entry name" value="VIRAL IAP-ASSOCIATED FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45809:SF3">
        <property type="entry name" value="VIRAL IAP-ASSOCIATED FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02114">
        <property type="entry name" value="Phosducin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosducin" evidence="2">
        <location>
            <begin position="36"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR22829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="269" mass="30962" checksum="778DFE1611141F1E" modified="2009-07-28" version="1">MQNEPLVKVDVDETEDTEWNDILRQHGVIPQRPPSPTAQLEEALEEALAKQHENRLEGLDFSDLEELEDEEDEEFLDIYRRKRMAEIQKLQEKSKFGQVFHINKPEYNKEITECSLGSKEKEDDGVYVFVHMSLESKMQSRILSSLFDQAARKFPQIKFVDIPANRAIENYPENNCPTLIVYYRGQVLKNLVTLLELGGNDTSITDLENLMVKVGAVDEKDERLAINADDDEAREERANRYTRKGIKSGITGKFNLGVGRDSDDDDFFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5DF96</accession>
    <name>C5DF96_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Phosphoribosylformylglycinamidine synthase</fullName>
            <ecNumber evidence="4">6.3.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Formylglycinamide ribonucleotide amidotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Formylglycinamide ribotide amidotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="ordered locus">KLTH0D13332g</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Phosphoribosylformylglycinamidine synthase involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide + L-glutamine + ATP + H2O = 2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17129"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the FGAMS family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.3" evidence="4"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553289.1">
        <property type="nucleotide sequence ID" value="XM_002553243.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DF96">
        <property type="interactions" value="1013"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DF96"/>
    <dbReference type="MEROPS" id="C56.972"/>
    <dbReference type="GeneID" id="8295531"/>
    <dbReference type="KEGG" id="lth:KLTH0D13332g"/>
    <dbReference type="eggNOG" id="KOG1907">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001031_0_2_1"/>
    <dbReference type="InParanoid" id="C5DF96"/>
    <dbReference type="OMA" id="LSANWMW"/>
    <dbReference type="OrthoDB" id="6666987at2759"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00128"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004642">
        <property type="term" value="F:phosphoribosylformylglycinamidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01740">
        <property type="entry name" value="GATase1_FGAR_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02203">
        <property type="entry name" value="PurL_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02204">
        <property type="entry name" value="PurL_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.750:FF:000002">
        <property type="entry name" value="Phosphoribosylformylglycinamidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.10:FF:000002">
        <property type="entry name" value="Phosphoribosylformylglycinamidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.10:FF:000005">
        <property type="entry name" value="Phosphoribosylformylglycinamidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000008">
        <property type="entry name" value="Phosphoribosylformylglycinamidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.650.10:FF:000002">
        <property type="entry name" value="Phosphoribosylformylglycinamidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.750">
        <property type="entry name" value="Phosphoribosylformylglycinamidine synthase, linker domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="entry name" value="PurM-like, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00419">
        <property type="entry name" value="PurL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040707">
        <property type="entry name" value="FGAR-AT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055181">
        <property type="entry name" value="FGAR-AT_PurM_N-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010073">
        <property type="entry name" value="PurL_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041609">
        <property type="entry name" value="PurL_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036604">
        <property type="entry name" value="PurS-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01735">
        <property type="entry name" value="FGAM_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003672">
        <property type="entry name" value="PRK05297.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10099">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10099:SF1">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18072">
        <property type="entry name" value="FGAR-AT_linker"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18076">
        <property type="entry name" value="FGAR-AT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22689">
        <property type="entry name" value="FGAR-AT_PurM_N-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13507">
        <property type="entry name" value="GATase_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01211">
        <property type="entry name" value="GATase_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109736">
        <property type="entry name" value="FGAM synthase PurL, linker domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82697">
        <property type="entry name" value="PurS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="6" id="KW-0436">Ligase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphoribosylformylglycinamidine synthase N-terminal" evidence="21">
        <location>
            <begin position="41"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoribosylformylglycinamidine synthase linker" evidence="20">
        <location>
            <begin position="205"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like C-terminal" evidence="19">
        <location>
            <begin position="470"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="domain" description="FGAR-AT PurM N-terminal-like" evidence="22">
        <location>
            <begin position="687"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like C-terminal" evidence="19">
        <location>
            <begin position="861"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="337"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1035"/>
            <end position="1056"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00052585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00057317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00071729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF18072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF18076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF22689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="CAR22851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1348" mass="146721" checksum="F8A21F9D1FF28C14" modified="2009-07-28" version="1">MSVLILPGPQALSPFRVDNLVKGIEAYCNSSSVISEVRACFVHYVDVAADATLSDKERQLLDALLTYDQALDPNDASSQQLKSAVVSGSKAHPDNDTYLVRILPRPGTISPWSSKATNIARVCGLDGKVGRIERGLALLIKTVPGFPLTENLNDVSLKSCYDRMTQQMFLDEPPKLDTIFSHDSPKPLGHVALVSDSSASPNEILSKANTELGLALDKGEMDYLIDAFVKTLHRDPSDVELFMFAQVNSEHCRHKIFNADWTIDGLQKQLSLFKMIRNTHKHTPDFTISAYSDNSAVLDTEHESYYFAPDFKTKEWKAVKEHVPMLVKVETHNHPTAVSPFPGAATGSGGEIRDEGATGRGSKSKCGLSGFSVSDLLIPGFKQPWELDVGKPNHIASALDIMIEAPLGSAAFNNEFGRPCINGYFRTLTTKVTNSDGKEEIRGFHKPVMIAGGFGAVRPQFALKNKPITPGSSLIVLGGESMLIGLGGGAASSINSGEGSADLDFASVQRGNPEMERRCQQVIDACISLDSGNPIQSIHDVGAGGLSNALPELVHDNDLGAKFDIRKVLSLEPGMSPVEIWCNESQERYVLGVSQQDLALFEDICRREKAPYAVVGHATAEQRLIVEDPLLKTTPIDLEMSILFGKPPKMTREAITQPLQLPAPDLKAVPSLEDAVQRVLSLPSVGSKSFLITIGDRTVTGLIDRDQFVGPWQVPVADVGVTGTALGEGICKTGEALAMGEKPTIALISAAASAKLSVAESLLNLFAADVKSLKHVKLSANWMSPASHQGEGSKLYEAVQAIGMDLCPDLDISIPVGKDSMSMKMKWSDKEVTAPLTLNITAFGPVEDTSKTWTPVLKKEEGTILVLVDLAASQDKLSLGGSALLQVYNQVGNTAPTVHNNKTFKNMLEALLELHKTDLVYAYHDRSDGGLFVALLEMAFASRSGVDIVLDSKLDSLTELFNEELGCVFQVSESRLPEFEDVLNKNGVPSEYISLVGRPNFKTQDVVVADKSGQVLFKNSRAALQKTWASTSYQVQKRRDNPQAADQEYSNISDDKDPGLHYSLAYDPTDDLGVGSLASASKPKVAILREQGVNGQMEMAWCFQQAGFTAIDVTMTDLLEGRFHLDEFVGLAACGGFSYGDVLGAGAGWAKSVLYHEGVRQQFVKFFNEREDTFAFGACNGCQFLSRLKEIIPGCENWPSFERNSSEQYEARVCMVEVAQDDGCKNESVFLNGMVGSKLPIAVAHGEGRAAFTSEAETKDFESQGLCSMRYVDNYGNTTSKYPFNPNGATNSIAGVRSPNGRVLAMMPHPERVCRLEANSWYPQEKYDEWQGYGPWIRMFRSARKWVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFX3</accession>
    <name>C5DFX3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0D00682p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0D00682g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552753.1">
        <property type="nucleotide sequence ID" value="XM_002552707.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFX3"/>
    <dbReference type="FunCoup" id="C5DFX3">
        <property type="interactions" value="986"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFX3"/>
    <dbReference type="GeneID" id="8294973"/>
    <dbReference type="KEGG" id="lth:KLTH0D00682g"/>
    <dbReference type="eggNOG" id="KOG2450">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_5_1_1"/>
    <dbReference type="InParanoid" id="C5DFX3"/>
    <dbReference type="OMA" id="GQLIMQY"/>
    <dbReference type="OrthoDB" id="310895at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019413">
        <property type="term" value="P:acetate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07091">
        <property type="entry name" value="ALDH_F1-2_Ald2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000011">
        <property type="entry name" value="ALD5p Mitochondrial aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000001">
        <property type="entry name" value="Mitochondrial aldehyde dehydrogenase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11699">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="7">
        <location>
            <begin position="54"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR22315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="522" mass="56627" checksum="09272F1451166DEA" modified="2009-07-28" version="1">MLSRTGTALRGMPVAATRLGMRFYAQLPLRVPVKMPNGIEYEQPTGLFINNKFVRAKQGKTFEVVSPATEEEITHVYEAREDDVEDAIAAADQAFKSGVWSDADPLVRAKALNKLAELIEEDLETIASIETLDNGKAISSARGDVQLVANYLRSCAGWCDKIDGRLINTGSTHFSYTIRQPLGVCGQIIPWNFPLLMWAWKVGPALATGNTVVLKTAESTPLSALYVSQFVPKAGIPAGVVNIISGFGKIIGEALTTHPKIKKIAFTGSTATGRHIYQNAGANLKKVTLELGGKSPNIVFADADLKATVQNIILGIYYNSGEVCCAGSRVYVEESVYDQFLEELKAATENVKVGDPFDDATFQGAQTSQMQLSKILKYVEIGRDEGATLVTGGERLGNKGFFVKPTIFGDVTEDMRIVKEEIFGPVVTITKFKTVEEVIEKANDSEYGLAAGVHTKDINKAIEVSNRVNSGTVWINTFNDFHHAVPFGGFNASGIGREMGIEALENYTQVKAVRAKLTLSGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFY0</accession>
    <name>C5DFY0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0D00836p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0D00836g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552760.1">
        <property type="nucleotide sequence ID" value="XM_002552714.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFY0"/>
    <dbReference type="FunCoup" id="C5DFY0">
        <property type="interactions" value="893"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFY0"/>
    <dbReference type="GeneID" id="8294980"/>
    <dbReference type="KEGG" id="lth:KLTH0D00836g"/>
    <dbReference type="eggNOG" id="KOG2046">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055232_2_1_1"/>
    <dbReference type="InParanoid" id="C5DFY0"/>
    <dbReference type="OMA" id="RLGPKMA"/>
    <dbReference type="OrthoDB" id="21595at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050606">
        <property type="entry name" value="Calponin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003096">
        <property type="entry name" value="SM22_calponin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47385">
        <property type="entry name" value="CALPONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47385:SF14">
        <property type="entry name" value="TRANSGELIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00888">
        <property type="entry name" value="SM22CALPONIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="2">
        <location>
            <begin position="34"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="162"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR22322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="208" mass="23358" checksum="5782D3A5E05DCC1A" modified="2009-07-28" version="1">MNSKHTGPSSYTAKADVTSLDQDLQELRRSKFSRESIDEISQWIFGAVLKEPIPSDLLPALKDSTALCRLANALHEADAPNSGPLIKFKESRMPFVQMEQISQFLSFARQYGVPEDELFQTVDLYEEKDPATVYQALKSLSRYANKKHPHQFPVIGPQIATKRPRPPKAAKPKHLQSSGWSTLEYGYIKGANQSSEGVVFGQKRDITH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFZ3</accession>
    <name>C5DFZ3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sorting nexin-3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0D01122g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Required for retention of late Golgi membrane proteins. Component of the retrieval machinery that functions by direct interaction with the cytosolic tails of certain TGN membrane proteins during the sorting/budding process at the prevacuolar compartment. Binds phosphatidylinositol 3-phosphate (PtdIns(P3)).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Prevacuolar compartment membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the sorting nexin family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552773.1">
        <property type="nucleotide sequence ID" value="XM_002552727.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFZ3"/>
    <dbReference type="FunCoup" id="C5DFZ3">
        <property type="interactions" value="527"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFZ3"/>
    <dbReference type="GeneID" id="8294993"/>
    <dbReference type="KEGG" id="lth:KLTH0D01122g"/>
    <dbReference type="eggNOG" id="KOG2527">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057172_2_1_1"/>
    <dbReference type="InParanoid" id="C5DFZ3"/>
    <dbReference type="OMA" id="NMYTDYE"/>
    <dbReference type="OrthoDB" id="5227681at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030904">
        <property type="term" value="C:retromer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034499">
        <property type="term" value="P:late endosome to Golgi transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051074">
        <property type="entry name" value="Sorting_Nexin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45963">
        <property type="entry name" value="RE52028P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45963:SF2">
        <property type="entry name" value="RE52028P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PX" evidence="14">
        <location>
            <begin position="36"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="15"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR22335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="160" mass="18602" checksum="4B157369D8295739" modified="2009-07-28" version="1">MRQFQSFSSSIESSLPQPHKQQSSSEIYAEPENFLEIEVTNPKTHFPHPDHRGMFTDYELICRTNVPAFTKRMSRVRRRYSDFELFRKCLLKELSLSSHPKIAVPHLPGKILLGNRFNDEVIQERMQSLNKWMIAVAGHPLLQSGSKVLVRFVEDEKFTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGE0</accession>
    <name>C5DGE0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="20">KLTH0D04554p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="ordered locus">KLTH0D04554g</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Actin patch</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552920.1">
        <property type="nucleotide sequence ID" value="XM_002552874.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGE0"/>
    <dbReference type="FunCoup" id="C5DGE0">
        <property type="interactions" value="333"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGE0"/>
    <dbReference type="GeneID" id="8295146"/>
    <dbReference type="KEGG" id="lth:KLTH0D04554g"/>
    <dbReference type="eggNOG" id="KOG0162">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000192_7_6_1"/>
    <dbReference type="InParanoid" id="C5DGE0"/>
    <dbReference type="OMA" id="PPEEYQM"/>
    <dbReference type="OrthoDB" id="6108017at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051286">
        <property type="term" value="C:cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051666">
        <property type="term" value="P:actin cortical patch localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:bipolar cellular bud site selection"/>
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        <property type="term" value="P:positive regulation of Arp2/3 complex-mediated actin nucleation"/>
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        <property type="term" value="P:response to osmotic stress"/>
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        <property type="term" value="P:reticulophagy"/>
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        <property type="entry name" value="MYSc_Myo1"/>
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        <property type="entry name" value="SH3_Myosin-I_fungi"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Kinesin motor domain"/>
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        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
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        <property type="entry name" value="Myosin_head_motor_dom-like"/>
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        <property type="entry name" value="Myosin_TH1"/>
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        <property type="entry name" value="P-loop_NTPase"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 15" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="9 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 15" id="KW-0505">Motor protein</keyword>
    <keyword evidence="10 15" id="KW-0518">Myosin</keyword>
    <keyword evidence="7 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 14" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="Myosin motor" evidence="18">
        <location>
            <begin position="36"/>
            <end position="715"/>
        </location>
    </feature>
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        <location>
            <begin position="771"/>
            <end position="966"/>
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        <location>
            <begin position="1119"/>
            <end position="1181"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
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            <begin position="588"/>
            <end position="610"/>
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            <begin position="951"/>
            <end position="1122"/>
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            <begin position="1174"/>
            <end position="1262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
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            <begin position="982"/>
            <end position="1006"/>
        </location>
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            <begin position="1030"/>
            <end position="1060"/>
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            <end position="1097"/>
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            <end position="1117"/>
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            <begin position="1200"/>
            <end position="1243"/>
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            <begin position="1250"/>
            <end position="1262"/>
        </location>
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            <begin position="129"/>
            <end position="136"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
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            <dbReference type="PROSITE" id="PS50002"/>
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        <source>
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        </source>
    </evidence>
    <sequence length="1262" mass="140269" checksum="661E4BD4CC6A62A1" modified="2009-07-28" version="1">MAIIKRGARNKTAQQPAKRSGQIKKATFESNKKKEVGVSDLTLLSKISDESINDNLKKRFQNGTIYTYIGHVLISVNPFRDLGIYTDSVLKSYMGKNRLEVPPHVFAIAESMYYNMKAYNENQCVIISGESGAGKTEAAKRIMQYIAAASNTHSDSIGKIKDMVLATNPLLESFGCAKTLRNNNSSRHGKYLEIRFNAQFEPCAGNITNYLLEKQRVVGQLKDERNFHIFYQFTKGASDNYRQLFGVQMPEQYIYTSASGCTSVDTIDDLSDFQATLKAMQVIGITQQEQDELFRLLSAILWIGNISFTENEEGNAQVCDTSVTDFVAYLLQVDAHFLTQALVERVMETNHGMKRGSVYHVPLNVVQATAVKDALAKAIYNNLFDWIVDRVNVSLQAFPGANKSIGILDIYGFEIFDYNSFEQICINYVNEKLQQIFIQLTLKSEQEEYAREQIQWTPIKYFDNRVVCDLIEARRPPGIIAAMNDSVATAHADSNAADQAFAQRLNLFSSNPHFELRSTKFIIKHYAGDVTYDIFGMTDKNKDQLQKDLVELIGTSSNTFLSSIFPTTVDKNSKRRPPSAGDKIIKSANELVETLSKAQPSYIRTIKPNQTKSPNDYDDHQVLHQVKYLGLQENVRIRRAGFAYRQTFDKFVERFYLLSPQCSYAGDYIWQGDTLDAVKLILKDASIPPSEYQLGVSKVFIKTPESLFALETMRDKYWYNMAARIQRAWRRFLLRRVDAASRIQRAIREKKGGNKYEKLRDSGTKLLAGRKERRSMSLLGYRAFMGDYLSCNEPKAKGAYIKRQAGINEKVIFSINGESLHSKFGRSALRLRKAFILTPSHFYVIGQNAVQNQITYTVDYKIDVKQIKYVSLTNLQDDWVGIFVANSTLPDPLINTYFKTELITHLKSLNKGIEVKIGPTLEYQKKPGKIHVVKCQISESAPKYGDIYKSSTISVRKGHPATSKSHPKPKKKSTYQDYAPQHRTSTSIGQPSIPTATRSTVVPTTGKQAPAKPPAPKIRQKKPAPPPPAKKQSQPAPSSNVANIAATAAQAAYHPQPAVHNSEQEAGVNHGTPPRQTPMGASQGSRSRQEPKSNFATASSPAKSAVPPPPPPPPPASKPSVPKFEAAYDFVGTGSPSELPLSKGDIVYISRQEPSGWSLAKTLDGAKEGWVPTSYMTEHKGTAHAVSSSRPSQSTQPGVSSSTENSASTATSATFTAHSSGPDQNSISNASSNPLASALAARANKVRQDSDEEEAEASEDDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGF4</accession>
    <name>C5DGF4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0D04884p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0D04884g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_002552888.1"/>
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    <dbReference type="eggNOG" id="ENOG502RZXR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091774_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGF4"/>
    <dbReference type="OMA" id="WYWTGSS"/>
    <dbReference type="OrthoDB" id="19981at2759"/>
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        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070072">
        <property type="term" value="P:vacuolar proton-transporting V-type ATPase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021013">
        <property type="entry name" value="ATPase_Vma12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31394">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 199"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31394:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 199"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11712">
        <property type="entry name" value="Vma12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="125"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="155"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR22496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="202" mass="23101" checksum="F83E9A08086AB92E" modified="2009-07-28" version="1">MFGIKLNSTLTKLLERLEGNAEAQDILEKGSISMKALLAFHSEHWSHVGVKELLTPLDFAFKKVPERGTAYTDEFKEHLEKLRSEQEEKDYQAMVRRDGLGAMLEDREELTPAQMAKQVKEQITTVFNVLVSVVSVVFAVWYWTGSSTHMEPPTRVLLCLFGGILVLVADVVVYNSYLRKIEEARVRERSKKERKTVVKTLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGF7</accession>
    <name>C5DGF7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0D04950p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0D04950g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552937.1">
        <property type="nucleotide sequence ID" value="XM_002552891.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGF7"/>
    <dbReference type="FunCoup" id="C5DGF7">
        <property type="interactions" value="43"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGF7"/>
    <dbReference type="GeneID" id="8295164"/>
    <dbReference type="KEGG" id="lth:KLTH0D04950g"/>
    <dbReference type="eggNOG" id="KOG0283">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008245_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGF7"/>
    <dbReference type="OMA" id="FHPNDDR"/>
    <dbReference type="OrthoDB" id="1932312at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040324">
        <property type="entry name" value="WDR44/Dgr2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14221">
        <property type="entry name" value="WD REPEAT DOMAIN 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14221:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="170"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="267"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="307"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="212"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="523"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="672"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="708"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="24"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="672"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="715"/>
            <end position="728"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR22499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="780" mass="86361" checksum="04FBA70CAB40A894" modified="2009-07-28" version="1">MHGPKPEFELSGSSTEDLILSKPSSQNSSETTSESSHSNGGDTESGRRSVGGATCREFTTHIAPVQFEMSKPSFKIQDFSTRVLGPKEEAKELQKAHTASDDPADPVLNNIFGRINREQFKRYLKQPAYLRVFNKQPRIKQFRRLFLAQELRLDNGDITSSSNTTLSTLSENNGRAVWATKFSRDGRFLATGGKDCTLRIWKVIASPLERNDLSNSTTKPQAKRISLRMPPSAATGRSNKDELDQVTAPGLMDLYAPVFHPLPYRTFQGHTQDILDLDWSKNGFILTTSMDKTARLWHCDRPKALKTFEHPDFVTCAKFHPNDDRFFLSGCLDHKLRLWSILDHSVSFEHYCGDIITAIDTSFGDGKYTAIGTFNGHISILYTRGLEMISTFHVMESSKGRIRKSTESGPKITGIEFFKSAPDNDLRIMVTSNDSRIRIFSVKNRTLLEVLRGFENAHSQISAHTLSTTRKKMYVIAPSENQWVYCWKLESSAGAADLDESSNNKAHRHGSIRGLLRRSLSIGSAHSSEKRSPPASDQLSGLTPSHKGKHEPIKNHHYIAFHAHHCTVTTTAVAPVNTAKTLALSDDFICELTMALSEGEDDVAVMRQQHRKSLLHTKKQHQRENDLLQKRFPSMIEAIGTIFVSTDSSGVIRVFRSDISTNVRKKVLSCLKRSSTPPKSESGSESNGSYMHSGLLGSTVKAATSTLTKSRALGSSPSIKSLKSVSPSNEAPTSASPQAPCDLCGSTDLTRTKGDPKLRLNTQAPVYICNSCGNQLVRAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGG0</accession>
    <name>C5DGG0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0D05038p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0D05038g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the p23/wos2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552940.1">
        <property type="nucleotide sequence ID" value="XM_002552894.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGG0"/>
    <dbReference type="FunCoup" id="C5DGG0">
        <property type="interactions" value="1231"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGG0"/>
    <dbReference type="GeneID" id="8295168"/>
    <dbReference type="KEGG" id="lth:KLTH0D05038g"/>
    <dbReference type="eggNOG" id="KOG3158">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078883_0_1_1"/>
    <dbReference type="InParanoid" id="C5DGG0"/>
    <dbReference type="OrthoDB" id="1564555at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051879">
        <property type="term" value="F:Hsp90 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051131">
        <property type="term" value="P:chaperone-mediated protein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06465">
        <property type="entry name" value="p23_hB-ind1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.790:FF:000055">
        <property type="entry name" value="HSP90 associated co-chaperone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007052">
        <property type="entry name" value="CS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045250">
        <property type="entry name" value="p23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22932:SF1">
        <property type="entry name" value="CO-CHAPERONE PROTEIN DAF-41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22932">
        <property type="entry name" value="TELOMERASE-BINDING PROTEIN P23 HSP90 CO-CHAPERONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51203">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CS" evidence="3">
        <location>
            <begin position="4"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="126"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="126"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00025733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR22502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="192" mass="21375" checksum="0560D21A04767C8F" modified="2009-07-28" version="1">MSPTITPEVLWAQRSSSTDAEKNYLLVTIVIPDCSEPSLDLESTHLEFKAKSPGHVGDEQEHQYHLRIDFFKEIDAKKSLGRVANGRNYFLKLYKKDLEEEYWPRLTKEKLKYHYIKTDFNKWVDEDEQEEQEEEDFGFPGAGAGAGGQDAQAAALQELLKGGGQGAGQQDQAAALQELLKNGGQGLGGEDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGJ9</accession>
    <name>C5DGJ9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0D05940p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0D05940g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552979.1">
        <property type="nucleotide sequence ID" value="XM_002552933.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGJ9"/>
    <dbReference type="GeneID" id="8295209"/>
    <dbReference type="KEGG" id="lth:KLTH0D05940g"/>
    <dbReference type="eggNOG" id="ENOG502QR14">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041456_0_1_1"/>
    <dbReference type="InParanoid" id="C5DGJ9"/>
    <dbReference type="OMA" id="YRYEKGH"/>
    <dbReference type="OrthoDB" id="69177at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004656">
        <property type="term" value="F:procollagen-proline 4-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045054">
        <property type="entry name" value="P4HA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044862">
        <property type="entry name" value="Pro_4_hyd_alph_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869:SF236">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE ALPHA SUBUNIT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13640">
        <property type="entry name" value="2OG-FeII_Oxy_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="6">
        <location>
            <begin position="131"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR22541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="232" mass="26353" checksum="97B22CB15B848322" modified="2009-07-28" version="1">MAKKVVKRLRVADKKARSETFFPNEIFELKHQASSFEFYPFPEPMLEGKVILIKKFFSDKISQALRSSFSSPGIMELHYQKGTKEYAERINDRLTTQNAEIAQILWERLLKCLRQDDYVMQELGFGQAKGLNPQIRAYRYEKGHKFAKHYDSSVVVSGIGSSRWTLLVYLSGGDDLVGGDTVFYAPHTGEATNIHPQTGLALLHKHGDDCLLHEAQVVSNGAKWVLRSDVVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGK8</accession>
    <name>C5DGK8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Class E vacuolar protein-sorting machinery protein HSE1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Class E vacuolar protein-sorting machinery protein hse1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0D06138g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the STAM family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552988.1">
        <property type="nucleotide sequence ID" value="XM_002552942.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGK8"/>
    <dbReference type="FunCoup" id="C5DGK8">
        <property type="interactions" value="387"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGK8"/>
    <dbReference type="GeneID" id="8295218"/>
    <dbReference type="KEGG" id="lth:KLTH0D06138g"/>
    <dbReference type="eggNOG" id="KOG2199">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010104_2_0_1"/>
    <dbReference type="InParanoid" id="C5DGK8"/>
    <dbReference type="OMA" id="QVYRDWW"/>
    <dbReference type="OrthoDB" id="10255964at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033565">
        <property type="term" value="C:ESCRT-0 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11805">
        <property type="entry name" value="SH3_GRB2_like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16978">
        <property type="entry name" value="VHS_HSE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000072">
        <property type="entry name" value="Unconventional Myosin IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050670">
        <property type="entry name" value="STAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002014">
        <property type="entry name" value="VHS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45929">
        <property type="entry name" value="JAK PATHWAY SIGNAL TRANSDUCTION ADAPTOR MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45929:SF3">
        <property type="entry name" value="JAK PATHWAY SIGNAL TRANSDUCTION ADAPTOR MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00790">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00499">
        <property type="entry name" value="P67PHOX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00288">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50179">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="VHS" evidence="10">
        <location>
            <begin position="14"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="9">
        <location>
            <begin position="212"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="182"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="376"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="422"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="182"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="198"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="396"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="422"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="453"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR22550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="489" mass="55852" checksum="2CE3F18FFE8ABA68" modified="2009-07-28" version="1">MSSNQSLRDAILRATDGKLRVDNWQFIIEVCDLVREDPEDRGRFATDIVEERLKEDDANVVLRTLSLVVSLAENCGSRLQQAISSKGFTKVLYGLIENPKVHLVVKREVAKVVEQLSDSFKNDSSLKSMQDLYVAISAQAPHLLVRERVPQKKEMSHETKRNEEKELEEALKLSILEYEQSSSQKAAQNNDNKVANQKHIESSHHNESQTQNTVRKVRALHDLTGRESDELSFRKGDIIVVIEQVYKDWWRGRLRGRVGIFPLNYVTPVAEKTPEQLRQQKAHEDAVFSQISNVDKLHRRMAENANDLELTQDGEVNNLYSVVTPLRPQLTKMIGDYAQKKEEYSSLRKVLADAEASYNRMLDRASQVYPTQSLPLERMGSGSYEQPVGLQHTHSRQASAVNPLSRQPQSGIPYVHAQSFLTGQPQPNQHQTHSLKLQQREQRQSPHQIPELYQQGPQNYQRQPQNTHPSETRPVHQVPYPMSNQATGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGM6</accession>
    <name>C5DGM6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0D06556p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0D06556g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the adaptor complexes medium subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553006.1">
        <property type="nucleotide sequence ID" value="XM_002552960.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGM6"/>
    <dbReference type="FunCoup" id="C5DGM6">
        <property type="interactions" value="163"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGM6"/>
    <dbReference type="GeneID" id="8295237"/>
    <dbReference type="KEGG" id="lth:KLTH0D06556g"/>
    <dbReference type="eggNOG" id="KOG2740">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026449_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGM6"/>
    <dbReference type="OMA" id="LNEMCDG"/>
    <dbReference type="OrthoDB" id="870at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030131">
        <property type="term" value="C:clathrin adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09252">
        <property type="entry name" value="AP-3_Mu3_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1170">
        <property type="entry name" value="Mu homology domain, subdomain B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050431">
        <property type="entry name" value="Adaptor_comp_med_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036168">
        <property type="entry name" value="AP2_Mu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001392">
        <property type="entry name" value="Clathrin_mu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018240">
        <property type="entry name" value="Clathrin_mu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028565">
        <property type="entry name" value="MHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10529">
        <property type="entry name" value="AP COMPLEX SUBUNIT MU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00928">
        <property type="entry name" value="Adap_comp_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005992">
        <property type="entry name" value="Clathrin_mu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49447">
        <property type="entry name" value="Second domain of Mu2 adaptin subunit (ap50) of ap2 adaptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00990">
        <property type="entry name" value="CLAT_ADAPTOR_M_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51072">
        <property type="entry name" value="MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MHD" evidence="7">
        <location>
            <begin position="197"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR005992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR22568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="456" mass="51005" checksum="4382D94E335F4B3C" modified="2009-07-28" version="1">MFLSFYITDLKNALVFQHLLSYDSPTFMSLWTKLQAMAPESPNDDSWFKVSIGRSLQVYRFKSKANKLIYWCLATHQSNPLEPAVFLEGFDNTLLQYFDKDQLTISKMVNNQDRIALLLNCMVDANEPAVTDFNHLRDMVPSREDLSKVINSTASSLSNRISQRNTSQVLNIGDPAKGPAAKEKLVPWRSAGVKYSNNELYVDVVETVHVILRKNEKTGELTSVRGVLDGVVDFRSHLSGNPVIALNLRLRGHDLGMPALHQCCLDSYQGRPDQLRFVPPDGKFQLMHYSIDLDSLQSKSKLFSNIGLVSPDYRTELGDLGDEFEVNLNIAGSQHAKVVDDLVITLSFGPLPSECKIKVLRSTHGHFENCASNNIGKWVFDKETPTGTLPVLRGCLENATREQVRALQLSVSYTNRGELPSGIKVDSVDILSGLSKNIKPFKGVKYIAKTGDFHLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGS1</accession>
    <name>C5DGS1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">dolichol kinase</fullName>
            <ecNumber evidence="3">2.7.1.108</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0D07634g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the polyprenol kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.108" evidence="3"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553051.1">
        <property type="nucleotide sequence ID" value="XM_002553005.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGS1"/>
    <dbReference type="FunCoup" id="C5DGS1">
        <property type="interactions" value="100"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGS1"/>
    <dbReference type="GeneID" id="8295283"/>
    <dbReference type="KEGG" id="lth:KLTH0D07634g"/>
    <dbReference type="eggNOG" id="KOG2468">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031307_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGS1"/>
    <dbReference type="OMA" id="KNWENTF"/>
    <dbReference type="OrthoDB" id="377083at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004168">
        <property type="term" value="F:dolichol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043048">
        <property type="term" value="P:dolichyl monophosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032974">
        <property type="entry name" value="Polypren_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13205:SF15">
        <property type="entry name" value="DOLICHOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13205">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 15-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="61"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="211"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="243"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="283"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="379"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR22613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="500" mass="56354" checksum="7FB8DD8F3D32C013" modified="2009-07-28" version="1">MEKQSAQDRERIQKLNEGYSPIHAFLKPEKVIQLMMLGSTIHLVVAKFLQNEAGAKLQNSLLVTSVAIFVGFWVSYRTRVANNGTWGSPLQPIATFVPDFNVVYLMYLPTLFSLIFAREVTIVNLVMSFNCTDLPVAMKLPVQAIFVLLNDEYQQNRTKNLKAIFINWVFASLIERIGQLRSFDRVDSNLFSICLTNVLFLLNRSSLHFQILHNVLKGFMVAISVNYLLSFITRKLNQYLRSVILLTSFIVVFPLTILCSFEVEGQNPAGWLFRYILSDSTRVKIIVGWLGCLLLLVPNIMIFQSKFSLDTSRKLWHFIILVLVVPPLHQDPEFVKIALAGTIVLFLSVEYLRYLKLAPFGEFFDSKLRSFADFRDDRGPIIISYIYLIIGIATPILINGSLVGVISLGVGDSLASIVGYRWGRHRWPGTSKTLEGTLAFITATSSCSLALKNFNNSFQDLSNAKLIMTCILSGILEGNSVLNDNILVPAFMLIVREGLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGW2</accession>
    <name>C5DGW2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0D08756p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0D08756g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553092.1">
        <property type="nucleotide sequence ID" value="XM_002553046.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGW2"/>
    <dbReference type="FunCoup" id="C5DGW2">
        <property type="interactions" value="32"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGW2"/>
    <dbReference type="GeneID" id="8295328"/>
    <dbReference type="KEGG" id="lth:KLTH0D08756g"/>
    <dbReference type="eggNOG" id="ENOG502S7JJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106810_1_0_1"/>
    <dbReference type="InParanoid" id="C5DGW2"/>
    <dbReference type="OMA" id="WLTTPMK"/>
    <dbReference type="OrthoDB" id="4069445at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="110"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="175"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR22654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="187" mass="20747" checksum="46D8F5056D0CC19D" modified="2009-07-28" version="1">MQGGVRRKNDLLPRYRGQAHSKKQSFLTTPMKKVAVYVMLLIMMYLAIKTVFVDPQNAETKLEIESAVGRAGAGGDALEAQMDAADLQTNPKVSKERFNNEVVMQQETKNLENEKDFQKQKVPTKNTKQEVGNDVKAVKPQFENKEQPPVDEQARRINEKAPYKKTDKKSDKAVAEGGAAAAAANAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGX1</accession>
    <name>C5DGX1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Putative tyrosine-protein phosphatase OCA1</fullName>
            <ecNumber evidence="3">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0D08976g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Putative tyrosine-protein phosphatase required for protection against superoxide stress.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein-tyrosine phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="3"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553101.1">
        <property type="nucleotide sequence ID" value="XM_002553055.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGX1"/>
    <dbReference type="FunCoup" id="C5DGX1">
        <property type="interactions" value="52"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGX1"/>
    <dbReference type="GeneID" id="8295338"/>
    <dbReference type="KEGG" id="lth:KLTH0D08976g"/>
    <dbReference type="eggNOG" id="KOG1572">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047845_2_2_1"/>
    <dbReference type="InParanoid" id="C5DGX1"/>
    <dbReference type="OMA" id="PWNPISE"/>
    <dbReference type="OrthoDB" id="6375174at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14531">
        <property type="entry name" value="PFA-DSP_Oca1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000035">
        <property type="entry name" value="Tyrosine phosphatase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020428">
        <property type="entry name" value="PFA-DSPs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004861">
        <property type="entry name" value="Siw14-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126:SF8">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE OCA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03162">
        <property type="entry name" value="Y_phosphatase2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01911">
        <property type="entry name" value="PFDSPHPHTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="11">
        <location>
            <begin position="40"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR22663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="205" mass="23254" checksum="7A6D40AEC9A40587" modified="2009-07-28" version="1">MEPDYEEDDDHVYINEETELGRENVVISHAPRARIVPPLNFCPVERYLYRSGQPSTVNFPFLLDLKLKTIIWLANEEPQDALLEFCDAHAIQLQFATINPDGGEDDNPWDGLTEHSIISALRTIVNVESYPLLVCCGMGRHRTGTVIGCLRRIMGWNLASVSEEYRRFTGSRGGRILVELLIEAFDTQLVEIDRAKAPQWLLTAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGX9</accession>
    <name>C5DGX9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">2-isopropylmalate synthase</fullName>
            <ecNumber evidence="6">2.3.3.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0D09152g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.13" evidence="6"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553109.1">
        <property type="nucleotide sequence ID" value="XM_002553063.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGX9"/>
    <dbReference type="FunCoup" id="C5DGX9">
        <property type="interactions" value="241"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGX9"/>
    <dbReference type="GeneID" id="8295346"/>
    <dbReference type="KEGG" id="lth:KLTH0D09152g"/>
    <dbReference type="eggNOG" id="KOG2367">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004588_3_0_1"/>
    <dbReference type="InParanoid" id="C5DGX9"/>
    <dbReference type="OMA" id="EYCNQME"/>
    <dbReference type="OrthoDB" id="418791at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003852">
        <property type="term" value="F:2-isopropylmalate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07942">
        <property type="entry name" value="DRE_TIM_LeuA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000045">
        <property type="entry name" value="2-isopropylmalate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.270:FF:000002">
        <property type="entry name" value="2-isopropylmalate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00572">
        <property type="entry name" value="LeuA_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013709">
        <property type="entry name" value="2-isopropylmalate_synth_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002034">
        <property type="entry name" value="AIPM/Hcit_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005668">
        <property type="entry name" value="IPM_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054692">
        <property type="entry name" value="LeuA-like_post-cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036230">
        <property type="entry name" value="LeuA_allosteric_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039371">
        <property type="entry name" value="LeuA_N_DRE-TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00970">
        <property type="entry name" value="leuA_yeast"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002991">
        <property type="entry name" value="PRK03739.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46911">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46911:SF2">
        <property type="entry name" value="2-ISOPROPYLMALATE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22615">
        <property type="entry name" value="IPMS_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08502">
        <property type="entry name" value="LeuA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00917">
        <property type="entry name" value="LeuA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110921">
        <property type="entry name" value="2-isopropylmalate synthase LeuA, allosteric (dimerisation) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89000">
        <property type="entry name" value="post-HMGL domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00815">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00816">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0432">Leucine biosynthesis</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="13">
        <location>
            <begin position="57"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR22671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="608" mass="67054" checksum="7B7C1845A1541D4F" modified="2009-07-28" version="1">MFRRTAVNLAQSAGKAIPPVKLAYKYMLKDPSKKYKPFKAVTLPNRKWPDNRITKAPRWLSTDLRDGNQSLPDPMSVEQKKEYFHKLVEIGFKEIEVSFPSASQTDFDFTRYAVENAPEDVSIQCLVQSREHLIKRTVESLSGAKRATVHCYLATSDMFRDIVFNMSQEEAIKKAVEATKLVRKLTKDDPAQQATRWSYQFSPETFSDTPPEFALEICEAVKAAWEPTEENPIIFNLPATVEVATPNNYADQIEYFSTHISEREKVCISTHAHNDRGCGVAASELGVMAGADRVEGCLFGNGERTGNVDLVTVALNMYTQGVSPELDLSDLNSVVEVVERCNKIPISPRAPYGGDLVVCAFSGSHQDAIKKGFALQEKRRANGDEEWKIPYLPLDPKDIGRDYEAVIRVNSQSGKGGAAWVILRSLGLDLPRNLQIEFSTTVQNRADSLGRELKSQEITELFKEVYNYSGNIGKVTLLEYDVTKIDAERRSLTGQVSINGGVYDIQGSGNGPISSLVDAMSNLFNVKFGVANYTEHSLGSGSSTQAASFIHLVYRRDSDNERTFKWGVGVSEDVGEASTKAILSAINSIVQSGDVSLPDAPSKASASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGZ2</accession>
    <name>C5DGZ2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transcription initiation factor IIA large subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0D09482g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFIIA subunit 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553122.1">
        <property type="nucleotide sequence ID" value="XM_002553076.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGZ2"/>
    <dbReference type="FunCoup" id="C5DGZ2">
        <property type="interactions" value="527"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGZ2"/>
    <dbReference type="GeneID" id="8295360"/>
    <dbReference type="KEGG" id="lth:KLTH0D09482g"/>
    <dbReference type="eggNOG" id="KOG2652">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030027_2_1_1"/>
    <dbReference type="InParanoid" id="C5DGZ2"/>
    <dbReference type="OMA" id="QKCTGEA"/>
    <dbReference type="OrthoDB" id="6275927at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005672">
        <property type="term" value="C:transcription factor TFIIA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07976">
        <property type="entry name" value="TFIIA_alpha_beta_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.18.10:FF:000007">
        <property type="entry name" value="TOA1p TFIIA large subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.100:FF:000001">
        <property type="entry name" value="Transcription initiation factor IIA subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.18.10">
        <property type="entry name" value="Transcription factor IIA (TFIIA), beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004855">
        <property type="entry name" value="TFIIA_asu/bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009088">
        <property type="entry name" value="TFIIA_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12694">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIA SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12694:SF8">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIA SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03153">
        <property type="entry name" value="TFIIA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01371">
        <property type="entry name" value="TFIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47396">
        <property type="entry name" value="Transcription factor IIA (TFIIA), alpha-helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50784">
        <property type="entry name" value="Transcription factor IIA (TFIIA), beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="89"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="149"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="96"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="114"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="153"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00074154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR22684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="224" mass="25193" checksum="7AD4A091B5407B43" modified="2009-07-28" version="1">MSNQEGARAYETIVENVVNEVREDFESAGIDEQTLQDLKLVWQTKLSESRVAKFMWDPEDEGALGTSLNNSNIAPSLLDSQGLAIPALSSGEDKAQQSQSQQQQAQQQPLMTQSKHEEQPKQEIELSMEDPDGEIAEQLKLQAKQAKKSALLDTDEINSDLDDSEDDYLNSSGEDEGQDENIVLCLYEKVLRVKNKWKCNLKDGLATINHKDYAFQRAQGETEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DH08</accession>
    <name>C5DH08_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0E00374p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0E00374g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553506.1">
        <property type="nucleotide sequence ID" value="XM_002553460.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DH08"/>
    <dbReference type="STRING" id="559295.C5DH08"/>
    <dbReference type="GeneID" id="8291641"/>
    <dbReference type="KEGG" id="lth:KLTH0E00374g"/>
    <dbReference type="eggNOG" id="KOG1404">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016922_10_0_1"/>
    <dbReference type="InParanoid" id="C5DH08"/>
    <dbReference type="OMA" id="GQMSCLL"/>
    <dbReference type="OrthoDB" id="10261433at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049704">
        <property type="entry name" value="Aminotrans_3_PPA_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45688">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45688:SF13">
        <property type="entry name" value="ALANINE--GLYOXYLATE AMINOTRANSFERASE 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR23069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="442" mass="48246" checksum="80130AB3F1DA6640" modified="2009-07-28" version="1">MESMTLEKNQYSYFDQSTFQKASQRLLNYGARFTPQVVTYAKGSYVYINDKKVLDFTSGQMSTLLGHGHPEIAETITKHASGLDHLYSGMVSPPVLELAEKLTSILPKGLDRAMFLSTGSEANEAAIKLAKNITGRFEVVGFSLSWHGMTSGSNGTTYQDGRKNQGPLMPGNLCLPAPNAYRSIFRKPDGSHDWETELDYGFSLIDAASVGSLAACIIEPVMSCGGMLVLPDGYLKALKKHCEKRRMLLIVDEAQTAIGRCGDMFAFESHGIVPDILTLSKTLGNGIPLSAVVTSDEISKKSAENGYLFYTTHINDPLPAAVGVKVLEIVQRDNLVPHSRQMGELFRKRLRELQKVYRQIGDIRGRGLMTGVELVDPDTLEADGQLAEKLASTMMKFGLSANLIAVPAFGGVFRIAPPISVSEEEILLGVEIIQKAFSEVIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHG1</accession>
    <name>C5DHG1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0E04070p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0E04070g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553659.1">
        <property type="nucleotide sequence ID" value="XM_002553613.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHG1"/>
    <dbReference type="FunCoup" id="C5DHG1">
        <property type="interactions" value="125"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHG1"/>
    <dbReference type="GeneID" id="8291808"/>
    <dbReference type="KEGG" id="lth:KLTH0E04070g"/>
    <dbReference type="eggNOG" id="ENOG502QSKI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019711_0_0_1"/>
    <dbReference type="InParanoid" id="C5DHG1"/>
    <dbReference type="OMA" id="IEAYQHC"/>
    <dbReference type="OrthoDB" id="434695at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034044">
        <property type="term" value="C:exomer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015374">
        <property type="entry name" value="ChAPs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31975">
        <property type="entry name" value="BUD SITE SELECTION PROTEIN 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31975:SF1">
        <property type="entry name" value="BUD SITE SELECTION PROTEIN 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09295">
        <property type="entry name" value="ChAPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="83"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="719" mass="79718" checksum="57A6C8CA3DC0864A" modified="2009-07-28" version="1">MCPRAAAAPPPLSPAALAAPRTNIEPPRRVREDEIGSAIEERRAKLPQFQDLGPPDLVSIVKYTPSGSANAHNNKRSSMQLEPSTSSPAAGSAVAAAGDLHVNDKHKGEVGTFLYCVGADTSDPTSIAVFLKGMADAIAEKPQVWFGRHKPYRVARITFATWNAFRQCDVEVVVHIPGAVQSYFLDTRGEVLAVAPDERELVWAETFASGVVRAIAMMADNREDGEVNNVVETRILNPLLSGEIGDVADHFIDVFPLLYARGVALGAPCDVACASRTNNYLCETLLHVTRLTRNFRRCRAMLEGMLGDHPEAVVLLARVLFAADLEVDAVRLIHDDLAAHGGVVTAHRSELLCVQAAFLLDAKRDYAAAQRLAQAAVDCAPSEFRPWHLLVQSYVGLNDIENALLALNGCPVAPHRDRYIFKRVISVAQDSANLHLPLPMDVVLDEVTGLNSRDIINEHKSVSPALLNLPAATLKANARTRYHLLTEIARKTGWESLLRYRSKLFVMEEEYQVAATPPAEEGDPLKPDGIRTKRLCERWLDNLFMVLYEDLKAYTLWQAEQLHFEAQNTHYSKTTTEWELLGLCALRLGHREEAAAAFEAGLQHRFSAESCRKLLQIYLHERSRLKKDSDLSSSQVTAAVLDYDNKIIDLCVKICCWNHRWYSEFSILLLDSLSVVVDDMGITKLSNEISSRFPETVVQLANDNLLAFYADYTDGGYDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHI9</accession>
    <name>C5DHI9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cytochrome b-c1 complex subunit 6, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Complex III subunit 6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0E04686g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UQCRH/QCR6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553687.1">
        <property type="nucleotide sequence ID" value="XM_002553641.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHI9"/>
    <dbReference type="STRING" id="559295.C5DHI9"/>
    <dbReference type="GeneID" id="8291836"/>
    <dbReference type="KEGG" id="lth:KLTH0E04686g"/>
    <dbReference type="eggNOG" id="KOG4763">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115913_0_1_1"/>
    <dbReference type="InParanoid" id="C5DHI9"/>
    <dbReference type="OMA" id="NEDCVEE"/>
    <dbReference type="OrthoDB" id="405848at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006122">
        <property type="term" value="P:mitochondrial electron transport, ubiquinol to cytochrome c"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.20:FF:000003">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.20">
        <property type="entry name" value="Ubiquinol-cytochrome C reductase hinge domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003422">
        <property type="entry name" value="Cyt_b-c1_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023184">
        <property type="entry name" value="Ubol_cytC_Rdtase_hinge_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036811">
        <property type="entry name" value="Ubol_cytC_Rdtase_hinge_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15336:SF0">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 6, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15336">
        <property type="entry name" value="UBIQUINOL-CYTOCHROME C REDUCTASE COMPLEX 7.8 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02320">
        <property type="entry name" value="UCR_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81531">
        <property type="entry name" value="Non-heme 11 kDa protein of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Ubiquinol-cytochrome C reductase hinge" evidence="12">
        <location>
            <begin position="98"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="26"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR23250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="169" mass="19099" checksum="0629EADF7ADD4AAC" modified="2009-07-28" version="1">MISSITEFLEEVKDAFVPTVAKAEEQAEESTDDDKSENDDKPEDDGEAAEGEAADAESADADAEAEGESDGDEGEDEDEDEDEDEDEDEDEDEEEQVDQMDALREECKNKEEAKPLVHHYMECVERVHKAQEDPGYADSEHKEDCVEEFFHLQHYIDGCVSPTLFDRLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5DI09</accession>
    <name>C5DI09_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="18">KLTH0E08756p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">KLTH0E08756g</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate to form deamido-NAD(+) (NaAD). Key enzyme in both de novo and salvage pathways for NAD(+) biosynthesis. Predominantly acts in the salvage pathways via NMN.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>beta-nicotinamide D-ribonucleotide + ATP + H(+) = diphosphate + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:21360"/>
            <dbReference type="ChEBI" id="CHEBI:14649"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="EC" id="2.7.7.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>nicotinate beta-D-ribonucleotide + ATP + H(+) = deamido-NAD(+) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22860"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <dbReference type="EC" id="2.7.7.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from nicotinamide D-ribonucleotide: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; NAD(+) biosynthesis; deamido-NAD(+) from nicotinate D-ribonucleotide: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the eukaryotic NMN adenylyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553857.1">
        <property type="nucleotide sequence ID" value="XM_002553811.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DI09"/>
    <dbReference type="FunCoup" id="C5DI09">
        <property type="interactions" value="567"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DI09"/>
    <dbReference type="GeneID" id="8292018"/>
    <dbReference type="KEGG" id="lth:KLTH0E08756g"/>
    <dbReference type="eggNOG" id="KOG3199">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033366_5_0_1"/>
    <dbReference type="InParanoid" id="C5DI09"/>
    <dbReference type="OMA" id="VPHGIQR"/>
    <dbReference type="OrthoDB" id="422187at2759"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00332"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000309">
        <property type="term" value="F:nicotinamide-nucleotide adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004515">
        <property type="term" value="F:nicotinate-nucleotide adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd09286">
        <property type="entry name" value="NMNAT_Eukarya"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000074">
        <property type="entry name" value="Nicotinamide-nucleotide adenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051182">
        <property type="entry name" value="Euk_NMN_adenylyltrnsfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005248">
        <property type="entry name" value="NadD/NMNAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045094">
        <property type="entry name" value="NMNAT_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00482">
        <property type="entry name" value="nicotinate (nicotinamide) nucleotide adenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12039">
        <property type="entry name" value="NICOTINAMIDE MONONUCLEOTIDE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12039:SF0">
        <property type="entry name" value="NICOTINAMIDE-NUCLEOTIDE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="17">
        <location>
            <begin position="223"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="51"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="53"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="103"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00053172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAR23420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="455" mass="51175" checksum="C15AADBB87C447B5" modified="2009-07-28" version="1">MDPTKAPDFEPPSENRTPNPPLAPQSTIPKSGPIMPYVLADYNASIDAPIHPRTYKKHSKAGRHVSTAHHSSIPLKQPELQPPSHETSDEDERAQDDHPAPSPALSAGPGSNPDHDSYFDNDAATISGSHYRRNSSRTSLAQQFTHQMNLFDGDSPFHNMSLKDEEPKEEHIGSRIHKYQIADLEEVPHGVVRQAATLDQYCFPSHRLQKELRNPNKLPLVVVACGSFSPITYLHLRMFEMALDAISEQTRFEVVGGYYSPVSDNYKKPGLAPSHHRVRMCELACERTSSWLMVDAWESLQPTYTRTAKVLDHFNDEINVKRGGIATSFGARVGVKIMLLAGGDLIESMGEPNVWADSDLHHILGNYGCLIVERTGSDVRSFLLSHDIMYEHRRNVLVIKQLIYNDISSTKVRLFIRRGMSVQYLLPNSVIRYIQEHGLYVNQTEPVKQVMGNKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>C5DI53</accession>
    <name>C5DI53_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein BZZ1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0E09790g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Plays a role in endocytosis and trafficking to the vacuole. Functions with type I myosins to restore polarity of the actin cytoskeleton after NaCl stress.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the BZZ1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553901.1">
        <property type="nucleotide sequence ID" value="XM_002553855.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DI53"/>
    <dbReference type="FunCoup" id="C5DI53">
        <property type="interactions" value="396"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DI53"/>
    <dbReference type="GeneID" id="8292065"/>
    <dbReference type="KEGG" id="lth:KLTH0E09790g"/>
    <dbReference type="eggNOG" id="KOG3565">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015390_1_0_1"/>
    <dbReference type="InParanoid" id="C5DI53"/>
    <dbReference type="OMA" id="TPMMEEP"/>
    <dbReference type="OrthoDB" id="8783038at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030864">
        <property type="term" value="C:cortical actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045010">
        <property type="term" value="P:actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11912">
        <property type="entry name" value="SH3_Bzz1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11778">
        <property type="entry name" value="SH3_Bzz1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000060">
        <property type="entry name" value="Actin polymerization protein Bzz1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000072">
        <property type="entry name" value="Unconventional Myosin IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035459">
        <property type="entry name" value="Bzz1_SH3_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15735">
        <property type="entry name" value="FCH AND DOUBLE SH3 DOMAINS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15735:SF21">
        <property type="entry name" value="PROTEIN NERVOUS WRECK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00611">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00055">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="F-BAR" evidence="10">
        <location>
            <begin position="5"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="9">
        <location>
            <begin position="488"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="9">
        <location>
            <begin position="566"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="164"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="429"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="456"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="548"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="175"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="429"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="468"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00054085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00061387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00074946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR23464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="622" mass="70037" checksum="D4AA86EB4874709B" modified="2009-07-28" version="1">MSSELSIGNEIKDGHKETYKWVLNNIKWLSELEAFYRDRAKLEKEYSEKLTHLAKEYFGKKSASTVSLSVGDMPTTTPGSLEAASLVAWNEVLSQTEMISKDHNQLSQEFEFQVADQIVALSKKCELLLTSINGFNTELTEKRDKAYSNLDKAKKTYDEKCVQMESARAKQTKTSSDKARRKADEREHEMNIAKNHYLITISQANRIKDKYYFQDVPEVLDLLQDLHESRTRVMNSIWISAGAVEREMNKRIDSRLTTVDSVVSQNKANLDTTMFVKHNLKEWKEPPDFQYVPSSVWHDDEHFVVPSNIELQDLKVRLAKAQQLYDQMNSFSQSEISGLSSLNKQKKEIKAQENVDAQSLLALLTKYTNVVSSYTSHETFKVEAEVEIESIQNNVAEEHDLNTEDIDLSNLRKKSGVFNRFKKSLTISSDKKSTHQDGGDAVSVLSSESKAKHHNRFSLFKGRDRAQSSASGASEVTSSNMNSIQDSSEHNKNRVLFQYQKQDNDEVSVSPGNAISLLTADSGSGWTRIKNDSTGEAGLVPTSYIEINEKPRSSAPRAPPPRKSGNAARTVKALFDYEAQGDDEISIRQGDLISVLKADDGSGWTYGELNGQKGLFPTNYCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DI57</accession>
    <name>C5DI57_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0E09878p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0E09878g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; biotin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553905.1">
        <property type="nucleotide sequence ID" value="XM_002553859.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DI57"/>
    <dbReference type="STRING" id="559295.C5DI57"/>
    <dbReference type="GeneID" id="8292069"/>
    <dbReference type="KEGG" id="lth:KLTH0E09878g"/>
    <dbReference type="eggNOG" id="KOG1401">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016922_4_3_1"/>
    <dbReference type="InParanoid" id="C5DI57"/>
    <dbReference type="OMA" id="RFLTINY"/>
    <dbReference type="OrthoDB" id="425114at2759"/>
    <dbReference type="UniPathway" id="UPA00078"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004015">
        <property type="term" value="F:adenosylmethionine-8-amino-7-oxononanoate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00834">
        <property type="entry name" value="BioA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049704">
        <property type="entry name" value="Aminotrans_3_PPA_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005815">
        <property type="entry name" value="BioA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00508">
        <property type="entry name" value="bioA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005940">
        <property type="entry name" value="PRK07986.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42684">
        <property type="entry name" value="ADENOSYLMETHIONINE-8-AMINO-7-OXONONANOATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42684:SF17">
        <property type="entry name" value="ADENOSYLMETHIONINE-8-AMINO-7-OXONONANOATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0093">Biotin biosynthesis</keyword>
    <keyword evidence="7 8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR23468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="517" mass="57924" checksum="17DF7185C516B9FF" modified="2009-07-28" version="1">MSACVPESSKNDYTDNSGTKNICPLECTQQASRSELWYIKALLVVLYVCLLGDRQFTIKRCLSSCNNAIHRRKTEHNNLPMDSITEQDLEFDRRHIWHPYASITEPLKVYPVRSAEGVYLHLQDGRKIVDGMASWWCVQHGYNNPRLNAAAVNQLKTMSHVMFGGIVHKPGIELCKKLVQMLPDPLECVVLADSGSISIDVGMKMALQYFNAKGKASKSKFLAIERGFHGDAFGALSVCDPNNSMHHMYGGFVPQNLFCKAPEVYFHDSSQDVEKKVEELDVAPFAKMIRENRESLAGVLMESIVQGAGGLRIYHPYFLKRVRELCTENDVLLILDEVATGFGRTGKLFAFEHSEIVPDIVCLGKALTGGYLTLSATVSTREVSEGICDGPAGRLMHGQTYMGNPLACAVANENLAILLEGKWKEQVRGIEAQLFRELAPLEKHRNVAEVRILGAIGIVEVNLYPVKVEELQRRFVDMGAWIRPFNKWIYIYPIYTITPEELRVLVKAIASVLDELT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIJ0</accession>
    <name>C5DIJ0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0E12892p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0E12892g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554038.1">
        <property type="nucleotide sequence ID" value="XM_002553992.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIJ0"/>
    <dbReference type="GeneID" id="8292206"/>
    <dbReference type="KEGG" id="lth:KLTH0E12892g"/>
    <dbReference type="HOGENOM" id="CLU_1896599_0_0_1"/>
    <dbReference type="InParanoid" id="C5DIJ0"/>
    <dbReference type="OMA" id="VKQDNDM"/>
    <dbReference type="OrthoDB" id="4036654at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013169">
        <property type="entry name" value="mRNA_splic_Cwf18-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08315">
        <property type="entry name" value="cwf18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="142" mass="16147" checksum="2387EA2B38C6F5FB" modified="2009-07-28" version="1">MSSLQEQIASRKRRLESLKGKHSDSDVQQPHPPNEPHGEEDTPVSKKPKELEEKVIESPVKQDNDMDPPSQNFPDPRSPTDESQEEGVDTDSGTDDEQTSKLTPTSDLKEKLRPQLDELEKRTQEAIKRLVRQRLLTQPDAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIT3</accession>
    <name>C5DIT3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0E15004p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0E15004g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554131.1">
        <property type="nucleotide sequence ID" value="XM_002554085.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIT3"/>
    <dbReference type="FunCoup" id="C5DIT3">
        <property type="interactions" value="346"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIT3"/>
    <dbReference type="GeneID" id="8292302"/>
    <dbReference type="KEGG" id="lth:KLTH0E15004g"/>
    <dbReference type="eggNOG" id="KOG0660">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_181_1_1"/>
    <dbReference type="InParanoid" id="C5DIT3"/>
    <dbReference type="OMA" id="GLYCFQE"/>
    <dbReference type="OrthoDB" id="192887at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009272">
        <property type="term" value="P:fungal-type cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000040">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="68"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="18"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="18"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR23694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="411" mass="46805" checksum="BD74FD145D3D63F5" modified="2009-07-28" version="1">MFTSVALQSNPNLINQAQNKSKPAIYSNKNTDVADASSPGTLRTPYTTTASNIRTIYQKANFSTPGNFDILQVLGKGSYGTVVSAIDKNGSGKPRRLAIKKVTNIFAREILLKRAIRELKFLNFFRGHKNIVSIVDLEIVTEKPYDGLYCYQELVEYDLARVIHSTVQFSEFHIRHFLYQILCGLKYIHSAEVVHRDLKPGNILCSINGTLKICDFGLARGIAQRHFGTKGAELSITNYVATRWYRAPELILSRKKYTKAIDMWAVGCILAEFYGRKPIFMGQDSLHQISEILEILGTPPRPIIAKYCSTRSWEIFSMRNEVKKRSWSSVYPFSCNHAHELLNALLDWDPDARPTAETALEHTFFVDLRNSEEEPICPHGTFDFSYENNFTSMTALRECLYDEVKAFKQQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJ88</accession>
    <name>C5DJ88_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0F14432p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0F14432g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554814.1">
        <property type="nucleotide sequence ID" value="XM_002554768.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJ88"/>
    <dbReference type="FunCoup" id="C5DJ88">
        <property type="interactions" value="30"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJ88"/>
    <dbReference type="GeneID" id="8293042"/>
    <dbReference type="KEGG" id="lth:KLTH0F14432g"/>
    <dbReference type="eggNOG" id="ENOG502RY3I">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054583_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJ88"/>
    <dbReference type="OMA" id="NDECGIA"/>
    <dbReference type="OrthoDB" id="4031940at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.280">
        <property type="entry name" value="alix/aip1 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038499">
        <property type="entry name" value="BRO1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035278">
        <property type="entry name" value="DUF5355"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17306">
        <property type="entry name" value="DUF5355"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR24377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="411" mass="46087" checksum="39E3C6957B79EBDC" modified="2009-07-28" version="1">MVMVHIISTSSCRFEGPWPQLGSSPMLVLHELPLQIPAGTQLSPELSLLRQDASRAVESGSDTSAMNKFDVHLQFASALLKSHAIFPDTELEELICIAVVNMAFFCQQSCERLVRKAYLEGSKEAWASSGQYNKKAIGLLKFLVSSRIASTTSLLGMVQTYIKCWTLSQQLSVVIFSLSKLRSQICGSSKADVDRYERLLGFQESDIKDLAKASLLYARLCIGCRETCSQLANTSLSSLCEPLCRYLDALTFVLMSLDCYRNDECGKAIGMLEAATDCLAKGLISNKQLKLIQETKKLKGKIASKFRELKSEGSKLQTKIKLFKRSTPIERGELHPFLQSTLEDFIIPLVMLLHHRYVNTNDKVFFQPVVNQREELTHSWPQGKTPELVGTAWFFDGEQLKENSPVNSEYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJD1</accession>
    <name>C5DJD1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0F15466p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0F15466g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554857.1">
        <property type="nucleotide sequence ID" value="XM_002554811.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJD1"/>
    <dbReference type="FunCoup" id="C5DJD1">
        <property type="interactions" value="1240"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJD1"/>
    <dbReference type="GeneID" id="8293088"/>
    <dbReference type="KEGG" id="lth:KLTH0F15466g"/>
    <dbReference type="eggNOG" id="KOG0533">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052367_2_1_1"/>
    <dbReference type="InParanoid" id="C5DJD1"/>
    <dbReference type="OMA" id="NEFGPIK"/>
    <dbReference type="OrthoDB" id="346839at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051229">
        <property type="entry name" value="ALYREF_mRNA_export"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19965">
        <property type="entry name" value="RNA AND EXPORT FACTOR BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19965:SF35">
        <property type="entry name" value="RNA ANNEALING PROTEIN YRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="67"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="162"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="182"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR24420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="213" mass="23273" checksum="25850C0583088DF9" modified="2009-07-28" version="1">MSANLDKSLDEIIGSNKKPIRKINKKAPKKVSKQIVGGGRRGASVAAVRQRAAPNKAASVLEGAYGTKVSVEGLPRDIKQDAVREFFATQIGGIARVLLSYNERGLSTGMATITFKSGEKAKEAVKKFNGAPIDGGKSRLRLSLIVDPTKKPLASRIQAVVERKPVPARGPNKKVAAVKKQKREEKKKQPKPEKKSLEQLDQEMADYFEEKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJI5</accession>
    <name>C5DJI5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0F16698p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0F16698g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554911.1">
        <property type="nucleotide sequence ID" value="XM_002554865.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJI5"/>
    <dbReference type="FunCoup" id="C5DJI5">
        <property type="interactions" value="36"/>
    </dbReference>
    <dbReference type="GeneID" id="8293144"/>
    <dbReference type="KEGG" id="lth:KLTH0F16698g"/>
    <dbReference type="eggNOG" id="ENOG502QUX9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024415_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJI5"/>
    <dbReference type="OMA" id="HINNCSE"/>
    <dbReference type="OrthoDB" id="4064138at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR24474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="676" mass="78399" checksum="43473EA71D768426" modified="2009-07-28" version="1">MILRSARSRPQFRPACVFFCPKQRGLHGDLKQRSTLEIINHVQSRIANEEYSSIKTFKNKLQRHYDSKYAGLLADMRCLGVMDEKLVKMIFMRRSGPTQHTAFVSSLESIKKGSNSVSEKRKRIYGIIKLQKALYPEIARTNGILVPETLHQWFWQNLRKDETFQHYYFLIQNDVLLSSKSSNLFLRRLLKGSEMEVQLGTFQVFLHNPEHHEIFQSKFEKLYNFSQINQIVTAVLARKDLRYIKLYFAALLNRLEGKELQNSDLSEKQRIALFIKFTNTLLNYLKLTCNYDMFLHSFKMVTDLVLAQKLDTRLLHKPFLLAIQYLRSLSQHSHVLKLISFAQELSLERSFKFKQSVIGELVSTMRAFNDPKIIVDYITTVYTSPKTLRLLNELGIWSLLRHNSVNVLNATQLERDFESNKIQKSHLSNFLAHRVLPNTVVLTELYRVILQYFSNGTPSTELKQLIIDLYHRYKACMINHEDYFVQPDCGIINVLIYHLRFQLKDHRLAFVLAQDFFQTQPFVKCDASTPFGLVLYHNHALTRTEISSLLVLMDKHKVKLDFKVISAMVFQNLRSGQIEEAHTWFQKLISAGFPLTHRSLIKCAIENDWELPRGIDTSFMTKAPTSVSNSRFEFEIDDAVSGGIMEEEEHESTVKFANALLGITETLAESKGTAGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJK1</accession>
    <name>C5DJK1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0F17072p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0F17072g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554927.1">
        <property type="nucleotide sequence ID" value="XM_002554881.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJK1"/>
    <dbReference type="FunCoup" id="C5DJK1">
        <property type="interactions" value="392"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJK1"/>
    <dbReference type="GeneID" id="8293160"/>
    <dbReference type="KEGG" id="lth:KLTH0F17072g"/>
    <dbReference type="eggNOG" id="ENOG502QPKX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049491_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJK1"/>
    <dbReference type="OMA" id="ANLDYEF"/>
    <dbReference type="OrthoDB" id="4081967at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035189">
        <property type="entry name" value="Std1/Mth1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17235">
        <property type="entry name" value="STD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="392" mass="44353" checksum="B585BD071A3C91AB" modified="2009-07-28" version="1">MFVLPPPPTSTKQALPALRSKRKGAELAPVPEDSEAKEFVNAPPEFASRARDEIRKRLLNRRPLQSDSHSDTVSLAPSETASTIVSATSDTSSAPSLRSSIFSAHVSLASSATSAAASYIEQEKPKLISLQEALPKVFYDMYASDLLMDPQNLLCNGRPKFTKRELLDWELNDIRSLLIVEALRPEWGFQLPIVHFEPPDSSASAIGLPQFRVQLLPLDSPDDFIIRTLVESDLYMEANLDYEFKITSAKYTVAAARKRHEQFTGYHEPVMHLSKPEWRNIIENYLLNIAVEAQCRFDFKKQCSDYKKWKQQQMLVKKPDMPPPSTIPPRKRESSLLKRTLLKNTALKLSEDKPPLPTSKVSLSKEEKAMLWSQCQSRVYQRLGLDWTPDGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJN4</accession>
    <name>C5DJN4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">KLTH0F17842p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0F17842g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
            <location evidence="1">Centromere</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the shugoshin family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554960.1">
        <property type="nucleotide sequence ID" value="XM_002554914.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJN4"/>
    <dbReference type="FunCoup" id="C5DJN4">
        <property type="interactions" value="196"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJN4"/>
    <dbReference type="GeneID" id="8293195"/>
    <dbReference type="KEGG" id="lth:KLTH0F17842g"/>
    <dbReference type="eggNOG" id="ENOG502QSMK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019322_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJN4"/>
    <dbReference type="OMA" id="HQPKTYR"/>
    <dbReference type="OrthoDB" id="5394106at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000779">
        <property type="term" value="C:condensed chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045132">
        <property type="term" value="P:meiotic chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011515">
        <property type="entry name" value="Shugoshin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011516">
        <property type="entry name" value="Shugoshin_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07557">
        <property type="entry name" value="Shugoshin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07558">
        <property type="entry name" value="Shugoshin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="8" id="KW-0137">Centromere</keyword>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Shugoshin N-terminal coiled-coil" evidence="11">
        <location>
            <begin position="46"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="Shugoshin C-terminal" evidence="10">
        <location>
            <begin position="388"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="130"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="323"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="377"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="432"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="513"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="576"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="10"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="175"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="323"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="342"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="354"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="451"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="468"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="542"/>
            <end position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR24523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="600" mass="67091" checksum="A76A0ACAF91C17C8" modified="2009-07-28" version="1">MAKTVRKRGSGTQLQVSPTHDSNDMPQIQQFQDLIDLQKSEFETIRSGYTQQNAQLAKSNSSLMIKVREMDKSVSELIQENVLLRSKLSMKELQFKERLTCHIQVMEKGVLQRFEEILHMFASIRRREGLEPPPENLMERSSFFSEPKSILKRNRASSGGKNPRGSTIAFDETENQVFSPQADRQQENASTRTEQIPSPPSKKRRKSSRRESLFHPSDFEFSDDEENANSSPGMDAVDSPQLPSEPITSNENVELDTARVGMESQQEPVEEPCNLTNSLIDYSIPEEVPEGEQESFSADTSSKVSVYRDNRDILNTRSIAYSTELSSERSPGNQGAAKDSQHPSFIQNSTSSQKKVKHSMKSRGVQKKMIDEVMPTTCGTTSELSDSSRSRRTRGRAINYALPSLRAKMRRPTEKLVDATTVTNIRDLQVVTGRTKSSSKSRDGTPFYDENSVQNLPPQPSNMIPGSDYEKPKNVTPTSHEKPKEASILNDAQESSANVESINVAKLNPLKDITNKAPAKPSNKTKKLFKKPIVGDLGDENSCSLDESSASHSASFRVNEEDLSVFDLLDDLKTNKAPKTHRARAGQATEKRGRKTAFRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJR7</accession>
    <name>C5DJR7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0F18568p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0F18568g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554993.1">
        <property type="nucleotide sequence ID" value="XM_002554947.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJR7"/>
    <dbReference type="FunCoup" id="C5DJR7">
        <property type="interactions" value="160"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJR7"/>
    <dbReference type="GeneID" id="8293228"/>
    <dbReference type="KEGG" id="lth:KLTH0F18568g"/>
    <dbReference type="eggNOG" id="ENOG502R4DC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_273636_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJR7"/>
    <dbReference type="OMA" id="DCKTIRH"/>
    <dbReference type="OrthoDB" id="4070435at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR24556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1167" mass="131637" checksum="84732AAD50AA7ABF" modified="2009-07-28" version="1">MVLDPQIGTFASKEETENCHWFDVSSYGSKLNKPGNEEKWFKESAELHSQLPIFCGNIRISLEEDKFKQLVGVRDEELKSFAPLVKLHEHFGLGPPADPESLDDSDLEFLAGQASERVSRIALPQETLQISKKFLVVSRNAMKLVDSSDAYACPFEIIACEIVRQPGFHEDVLIILTEKGTLYTVAFDADFRPFVLQWWHSADFVDTPRALNVHPCFGEFLVTTDYNLQFFKFTSNYRFELISNLTFENSIITGSTFLHNGYRPDHLMAFLALSLSDRITLCVAQWGNVHEERKEVHPLSLLKSDPITSIICLGDTRCLIFTNKEIGLVTANQIISGELPIPCFQRSRLGDEVRKCFDDPDLLSMLKSIRPELNDFVHCTVLATSNGALCCCLIDDKDNTKFLALTRFKGLCNAFLALNSCKPGNFYAVIISSFGRLFEILLDLTTIEELVKDYKIPALQNIVSRRTLYAGHIAGDSLLYVAPSQIYTTKQDCLLLLSQATVSRITVGEPNLSCSRLLTLRSFKTLNKLCIIDCSTLPEKWKKHFLDQDASADVLRFFMIEKTPAGSSNLILLEWDPGHKVSRSRELDGVLRDEKRDTVLFFFTEENIIQVTPRTIYVDDLEETGRYEHFTSWPLTGALNNGKMLIAWNSETGKMLFCPDIESASSENFFEFTCPMENERSVITAVSFIRASSMRAWVTVVCDGTVFYFDCSAISDGIEPVRWFTDHRIVFGNSCFEGSMFCNLEGEMYYCSHTPPIGDWHLSRMNIEIMHKGPLEIRFLNTELCLVFAPQNMLLLDLRKQSATNIPLVDGRRNSIILDVQCVRGLYFVLFSDGLEILEADCFTNTPTQIVVKATRIKGKKYFYSRKINRMLIANLRKKCLDCAKLENGKLMSLDSKCMTDFVTVFDIITLPGDSRAIFLVIAGTIPASDSKFALKLIEISPSPGKLVVREVSYKAVDADDIGHVQMRASAANKFWISCGKAVQLLQLKPDGFDFVRKSETLPHGICCFDATNDLLVAATENDELYGECRILDGDWRALNMINLHGVKGRLKVNIINDMCVAVYGDITDKKGALVSQIFFYRVVGARLECYNTITFKESIRDVQYSQKNNELCVLQEDGVIRLFQGVEKEHEIQGIIGQPESICFSHASSQSGCWRISATELSPVTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJS6</accession>
    <name>C5DJS6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">tRNA (guanine(10)-N(2))-methyltransferase</fullName>
            <ecNumber evidence="9">2.1.1.214</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0F18788g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM11 methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.214" evidence="9"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555002.1">
        <property type="nucleotide sequence ID" value="XM_002554956.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJS6"/>
    <dbReference type="FunCoup" id="C5DJS6">
        <property type="interactions" value="892"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJS6"/>
    <dbReference type="GeneID" id="8293238"/>
    <dbReference type="KEGG" id="lth:KLTH0F18788g"/>
    <dbReference type="eggNOG" id="KOG2671">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029646_3_0_1"/>
    <dbReference type="InParanoid" id="C5DJS6"/>
    <dbReference type="OMA" id="AFNKWSR"/>
    <dbReference type="OrthoDB" id="296065at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043527">
        <property type="term" value="C:tRNA methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160102">
        <property type="term" value="F:tRNA (guanine(10)-N2)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000260">
        <property type="entry name" value="RNA methylase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000241">
        <property type="entry name" value="RlmKL-like_Mtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016691">
        <property type="entry name" value="TRMT11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059073">
        <property type="entry name" value="TRMT11_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13370">
        <property type="entry name" value="RNA METHYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13370:SF3">
        <property type="entry name" value="TRNA (GUANINE(10)-N2)-METHYLTRANSFERASE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25904">
        <property type="entry name" value="Tmrp11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01170">
        <property type="entry name" value="UPF0020"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017259">
        <property type="entry name" value="tRNA_mtfrase_TRM11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51627">
        <property type="entry name" value="SAM_MT_TRM11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 10" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="3 10" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="tRNA (guanine(10)-N(2))-methyltransferase TRMT11 N-terminal" evidence="12">
        <location>
            <begin position="3"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosomal RNA large subunit methyltransferase K/L-like methyltransferase" evidence="11">
        <location>
            <begin position="189"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00066937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF25904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR24565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="437" mass="50001" checksum="640F46F7764359DE" modified="2009-07-28" version="1">MVKKYLLFMVQTHLNFRLAELESLADLFGVKTVDFSKYDEGSPFFIVELENDAQARAWISRSILTRGIYEYWGQGTTYEELHREIQANPLFKQFKAEYRNSTFKFEFDSFGGSSSGKLDRVACIESFKYLEFQGKIRMKNPDQVFTVIEEYEPLSDNVAGEKALRMFFGREVQKSDRLCGALEKYDLKKRPYKGTTSFEAELSLVSANIAQVKPGTVMYDPFAGTGSFLVAGGHYKALVIGSDIDGRMIRGKGSQTIKANFKRYGESLQFLDVVTMDFTHNALRSDFLIDTILCDPPYGIRESIKVLGARDPERFVGKEDVEIDGKKAYLHRDYIPTKKPYSLDALLDDLLQFASQRLPLNGRLAFWMPVANDENIETVIPLHANLELKYNCVQEFNKWSRRLLVYVNRGDGFQGPTNAGLERSKANFRDRYFSGFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJW3</accession>
    <name>C5DJW3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0F19602p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0F19602g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555039.1">
        <property type="nucleotide sequence ID" value="XM_002554993.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJW3"/>
    <dbReference type="FunCoup" id="C5DJW3">
        <property type="interactions" value="42"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJW3"/>
    <dbReference type="GeneID" id="8293275"/>
    <dbReference type="KEGG" id="lth:KLTH0F19602g"/>
    <dbReference type="eggNOG" id="ENOG502RXY0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_327902_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJW3"/>
    <dbReference type="OMA" id="QYAQHTS"/>
    <dbReference type="OrthoDB" id="4060030at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950403" evidence="2">
        <location>
            <begin position="21"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="612"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="750"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="612"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="644"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="660"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="762"/>
            <end position="773"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR24602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1000" mass="104512" checksum="24A42ACF504D80E3" modified="2009-07-28" version="1">MVSLRNIFVGASCIVGLASAKYSNTSSSTVPSVPKTTPTAFFSSVHLVDSYTGTNSSNDKDHWFLVKAEVYISADYNSELYMTVPDDFQDVPQDSFDLLQGSNVVGTVEHNSSNVLAIKPKVSEDDRTATFNFLTRLSGSAKDSITSPTTVDYIFNVSSGPSIVETIDFVATNLSASQTNARVENDNSVTYTLDIPLSEYPGSFNLAASFISGNFTFDTSKTRVEAVLDIDDFNQPIKAINLTAFTDNSDESIINLSMGSQLSGGKFIRITYATMPVSGFAIVDTVSILNYPSLSNYKRDVSIVFEDYVPVSSAANIQDYGEQISVVSTSSFSKASPTATAGNSTSARSTSLYSVTLSQSTNTQRGNASLSTSGASFTATNVTLASTSTSVPTNVSKTITSALSIATGSVHINNTSGHALTTTNATTTSIPGSVSIFTSSDSSILANKTAASSAISTHNSSITTTTLITNSTSALSKNSSMPSQDDSVKYTYTVITQTSNGEFLVYTSFYPVATLSPSATARVSSTSESIYVINATLSYTRGYNSTGLSTNAFATNSLSTATKSGEFYEYTSLVPVATLSQDIKSSVIHTGGVSSRLSSGFSTRTKEIPSASTSRFTSTSSVSKKSSRGSLTHTKDASSKSSSRIPTSTKGFSSKASHGSSEKDRVSTEYRTERYLTNSVYTATSNGRVVEYTSWYPVTSVSPEKVTIKDDSAWAYTLSTEGGTLISSTATYTIGGQVSVITTLVPVNTETSKKASSRPGASPTKKSSVESSTTKHYLTTSTSCGSKACKTFTTYVDSAEHTQQYSTQVGVSANIGQSALGPDVTSYNSLLGSSTLPASSTSKGNTAVSFSGTNTKVSTNIVASSNGPVVTTYESLVGGSAGAKSSKTTIKSTKTGAMSTATTEASFSTSGADLSAQAFTSSVRVATSYSQSTGVEALSTGEVFIGQSTLSEKIASTGFQTQTVSGYFSSSVEVSTYDGAAAKIEGRLVGMIIGLSALFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJW4</accession>
    <name>C5DJW4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0F19624p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0F19624g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
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                <person name="Talla E."/>
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            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24603.1"/>
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        <property type="nucleotide sequence ID" value="XM_002554994.1"/>
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    <dbReference type="AlphaFoldDB" id="C5DJW4"/>
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    <dbReference type="STRING" id="559295.C5DJW4"/>
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    <dbReference type="eggNOG" id="KOG0723">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017633_13_4_1"/>
    <dbReference type="InParanoid" id="C5DJW4"/>
    <dbReference type="OMA" id="KLMVMNH"/>
    <dbReference type="OrthoDB" id="240298at2759"/>
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        <property type="component" value="Chromosome F"/>
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        <property type="term" value="C:PAM complex, Tim23 associated import motor"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="F:ATPase activator activity"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:protein import into mitochondrial matrix"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    <keyword evidence="3" id="KW-0813">Transport</keyword>
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            <begin position="84"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
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    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00271"/>
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    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR24603.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="145" mass="16268" checksum="82405C3D6EB89FA6" modified="2009-07-28" version="1">MVLPIIIGVGVTFAAVTARSGIRAWEVYQKLTPLMIARLNRIRLTEAEINFQRESSVRFRNLSPTLKARLDQYRGGFSARMTEAEALLILGISGSEVASLNEQLLKRKHHKAMVQNHPDRGGSPFLAMKINEARDVLCHSVMLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJX6</accession>
    <name>C5DJX6_LACTC</name>
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        <submittedName>
            <fullName evidence="16">KLTH0F19888p</fullName>
        </submittedName>
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    <gene>
        <name evidence="16" type="ordered locus">KLTH0F19888g</name>
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    <organism evidence="16 17">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
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                <person name="Talla E."/>
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            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
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        <subcellularLocation>
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            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_002555006.1"/>
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    <dbReference type="eggNOG" id="ENOG502QPQC">
        <property type="taxonomic scope" value="Eukaryota"/>
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        <property type="component" value="Chromosome F"/>
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        <property type="term" value="C:side of membrane"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2430">
        <property type="entry name" value="Agglutinin-like protein, N-terminal domain, N2 subdomain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Adhesion_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024672">
        <property type="entry name" value="Agglutinin-like_N"/>
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    <dbReference type="InterPro" id="IPR043063">
        <property type="entry name" value="Agglutinin-like_N_N2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033504">
        <property type="entry name" value="ALS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011252">
        <property type="entry name" value="Fibrogen-bd_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001389">
        <property type="entry name" value="Flocculin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33793:SF2">
        <property type="entry name" value="AGGLUTININ-LIKE PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33793">
        <property type="entry name" value="ALPHA-AGGLUTININ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11766">
        <property type="entry name" value="Candida_ALS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00624">
        <property type="entry name" value="Flocculin"/>
        <property type="match status" value="15"/>
    </dbReference>
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        <property type="entry name" value="Candida_ALS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Bacterial adhesins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-0134">Cell wall</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950508" evidence="14">
        <location>
            <begin position="22"/>
            <end position="1406"/>
        </location>
    </feature>
    <feature type="domain" description="Agglutinin-like protein N-terminal" evidence="15">
        <location>
            <begin position="50"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1016"/>
            <end position="1081"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1148"/>
            <end position="1177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1191"/>
            <end position="1381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1016"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1045"/>
            <end position="1081"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1148"/>
            <end position="1171"/>
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    </feature>
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        <location>
            <begin position="1194"/>
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        <location>
            <begin position="1284"/>
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        <location>
            <begin position="1297"/>
            <end position="1339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1346"/>
            <end position="1362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1363"/>
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        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00004589"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
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            <dbReference type="SMART" id="SM01056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAR24615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1406" mass="145402" checksum="D66F8161C981DDED" modified="2009-07-28" version="1">MSSILISYLTTALCVLALTSAKTVSGIFNALSITPPSSLYPETLWTAKLSYAVSSSMGVNQGDTFSIDLPYVLKIYGEVTIGSSSYITISDQSGNTIANCLITNAFTDSASSSLTCTFSRSGIQSASGSISFDFVFLDGGSMSAGAVAAANNWKLGTNTLSWDGLTATVSFKTGGRATVPVTASRFTGYDNQLPAPSNSELCYYVGRACATQAFNEVVSIVYRGAPNNNGALDSCSGVTVAMANSFNAWNMPMNPVTIPSASYKLSCSSNTVVVSATSIPAGYHIFVDAYQVVSRDYSGAISFTNTYYESLACGKSTIHTGYARVLGFTSGSASGAGSGKYATPTTTTAGWSSSYTSTTTSIQAATGSDGNLYPQTVVVIEVPTVGSTSLSYSRWTATQESTYSTALLSTTGKDGTITVTTVYYVETPISRAQSTTMMPQSVPSTSTFSTNVFTTTGADDLPTLETLYYVWTPIVALTSTTSAPYSGAETSTYATELTTFTGEDGKQTVETIYYVETPIVDVTSATTRPYSGAETTTYTTELTTFTGEDGKQTVETIYYVETPIVDVTSATTRPYSGAETTTYATELTTYTGEDGKQTVETIYYVETPIVDVTSATTRPYSGAETTTYTTELTTFTGEDGKQTVETIYYVETPIVDVTSATTRPYSGAETTTYATELTTYTGEDGKQTVETIYYVETPIVDVTSATTRPYSGTETTTYATELSTFTGEDGKQTVETIYYVKTPTRATTVFTGWTGTETSTFSTGLTTFTGSDGEPTTETVYYVETPTRATTVYTGWTGTETSTFSTDLTTFTGSDGEPTTETVYYVETPTRATTVYTGWTGTETSTFSTDLTTFTGPDSEPTTQTIYYVETPTRATTVYTGWTGTETSTFSTDVTTFTGPDGEPTTQTVYYVETPTRATTVYTGWTGTEISTFSTDVTTFTGSDGEPTTETVYHVETPVVATIVYTRWTGRFTSTFSTGVSILTGPNGQATTQTVYYVETPASAYYGNSTSFSQDSGSTLTSVPSSTDKTSSAPGFSSAQPTTQSSSKSNLPVPSSTSSSTATASSTSASSASSSADSGTSTVVVSTPVSASATTTSLFSSSQESSVTEASSSSALVTSAVVVSTPAPTSGTTTSLFSSSQESSVTEASSSSASGTLSGATSSTTKGSLASDSTETVTDGNVVTSTVAVAPGSKSYSNTGLTSTPASVSQTGTATGPEYLTTGPAIGSATGPATGPGPATNSVTASPSSQIPKPSATESSSPASIEQISVSPEASSSVPNAMNSATQAAATTSSASLGGNTPVAVNSGTTSTKQIESAPSAPENHSNKTGDSGTESDTATKAAMGASSSALGWHSGQTTTVAPSSQLSASASTTSPSRHSSSVSVYEGAASRIGLGFLFALPLALI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5DK65</accession>
    <name>C5DK65_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Post-GPI attachment to proteins factor 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0F02134g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in the lipid remodeling steps of GPI-anchor maturation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Endoplasmic reticulum membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the PGAP3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554303.1">
        <property type="nucleotide sequence ID" value="XM_002554257.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DK65"/>
    <dbReference type="FunCoup" id="C5DK65">
        <property type="interactions" value="191"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DK65"/>
    <dbReference type="GeneID" id="8292495"/>
    <dbReference type="KEGG" id="lth:KLTH0F02134g"/>
    <dbReference type="eggNOG" id="KOG2970">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032917_1_1_1"/>
    <dbReference type="InParanoid" id="C5DK65"/>
    <dbReference type="OMA" id="DFMIEDC"/>
    <dbReference type="OrthoDB" id="419770at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007217">
        <property type="entry name" value="Per1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13148">
        <property type="entry name" value="PER1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13148:SF0">
        <property type="entry name" value="POST-GPI ATTACHMENT TO PROTEINS FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04080">
        <property type="entry name" value="Per1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="2 7" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5016485101" description="Post-GPI attachment to proteins factor 3" evidence="7">
        <location>
            <begin position="20"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="156"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="186"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="217"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="246"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="284"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR23866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="354" mass="41121" checksum="C7976E94FB887AEA" modified="2009-07-28" version="1">MLCLWVFLTLSGLVQRTLGSPGDWLDEFIDCKELCEASIPCAGFEGIEIPSGASAEITYFTEASAIQKYFLFWDCKSNCDYQCQQVVTQIRIAEGEKVVQFHGKWPFKRLFGMQELFSTLFSVANFFPHYRGYKLLQRELSRLPARKRSRFILKKYLYVAIAGMLAWTSSSIFHFRDLEVTEKLDYFFAGATVLSGFHGILIRILRLDKSDQFRHAVTAAVLLIFSLHVLRLYLDWSYTYNMRFNVLFGLLQYILLLTLAYKNYRQLKAGKLPRKSHHPPRENLYFELCVVPVALVVGTALAMSCELFDFFSYSWQIDSHAIWHACTVLPSWKLYDFFLHDFHYLDACEDSSSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKD8</accession>
    <name>C5DKD8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein SOP4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0F03828g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the export of PMA1, possibly through the monitoring or assisting of PMA1 folding and acquisition of competence to enter vesicles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SOP4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554376.1">
        <property type="nucleotide sequence ID" value="XM_002554330.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKD8"/>
    <dbReference type="FunCoup" id="C5DKD8">
        <property type="interactions" value="44"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKD8"/>
    <dbReference type="GeneID" id="8292570"/>
    <dbReference type="KEGG" id="lth:KLTH0F03828g"/>
    <dbReference type="eggNOG" id="ENOG502RXGD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102669_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKD8"/>
    <dbReference type="OMA" id="PYITVEL"/>
    <dbReference type="OrthoDB" id="27095at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031395">
        <property type="entry name" value="Sop4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17081">
        <property type="entry name" value="SOP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR23939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="270" mass="30470" checksum="43C42E37AE13F365" modified="2009-07-28" version="1">MTFYELSLVRGTKRVQAGTRRLFWTRRFDMLLLLFCLISGLCSSTLAATVSGRLDVSPLNITRKEVAHSSFKLLQVGNFTGPAYYSQTTVRDVHGNFKFEGVPEPKDANSSTYFVLQPSSLDYNLKPGRILVQLVRDPEDPQKIVTKAFKNSFGRENFPSPEILYPEKLAEVAADPCITFSLVNAAPFREYITERNAGILKSGPVASILNSKFKMAAVITAVLMLLFPYLLEKFDSETAQAIKDDRLEKQKRKYADQKRVGEQAQRLEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKF1</accession>
    <name>C5DKF1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EKC/KEOPS complex subunit GON7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0F04158g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. GON7 likely plays a supporting role to the catalytic subunit KAE1 in the complex. The EKC/KEOPS complex also promotes both telomere uncapping and telomere elongation. The complex is required for efficient recruitment of transcriptional coactivators.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the EKC/KEOPS complex composed of at least BUD32, CGI121, GON7, KAE1 and PCC1; the whole complex dimerizes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GON7 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554389.1">
        <property type="nucleotide sequence ID" value="XM_002554343.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKF1"/>
    <dbReference type="FunCoup" id="C5DKF1">
        <property type="interactions" value="39"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKF1"/>
    <dbReference type="GeneID" id="8292584"/>
    <dbReference type="KEGG" id="lth:KLTH0F04158g"/>
    <dbReference type="eggNOG" id="ENOG502S429">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_151420_1_0_1"/>
    <dbReference type="InParanoid" id="C5DKF1"/>
    <dbReference type="OMA" id="QDHLNIF"/>
    <dbReference type="OrthoDB" id="2288868at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014849">
        <property type="entry name" value="EKC/KEOPS_Gon7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08738">
        <property type="entry name" value="Gon7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0010">Activator</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0779">Telomere</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="107"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="14"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="28"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="114"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00025393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR23952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="132" mass="14747" checksum="EB345527E74910E7" modified="2009-07-28" version="1">MPSPFAIYKSPNSEPREFRVDPARDRYNTTNGTTTGPSAYVLQAGQVDRDKPSEPKCNEQGEFTELSILRMALTGLQDDINEYLTQQMEVAKNKKLKQDDEQRISGEINKLLDGGDDDDDDDDDQGSSSKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKH3</accession>
    <name>C5DKH3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cytochrome b-c1 complex subunit 8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Complex III subunit 8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0F04664g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The complex plays an important role in the uptake of multiple carbon sources present in different host niches.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII), a multisubunit enzyme composed of 3 respiratory subunits cytochrome b, cytochrome c1 and Rieske protein, 2 core protein subunits, and additional low-molecular weight protein subunits. The complex exists as an obligatory dimer and forms supercomplexes (SCs) in the inner mitochondrial membrane with cytochrome c oxidase (complex IV, CIV).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Mitochondrion inner membrane</location>
            <topology evidence="1 11">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the UQCRQ/QCR8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554411.1">
        <property type="nucleotide sequence ID" value="XM_002554365.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKH3"/>
    <dbReference type="FunCoup" id="C5DKH3">
        <property type="interactions" value="117"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKH3"/>
    <dbReference type="GeneID" id="8292606"/>
    <dbReference type="KEGG" id="lth:KLTH0F04664g"/>
    <dbReference type="eggNOG" id="KOG4116">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_156007_0_1_1"/>
    <dbReference type="InParanoid" id="C5DKH3"/>
    <dbReference type="OMA" id="AGIYWYW"/>
    <dbReference type="OrthoDB" id="6683853at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045275">
        <property type="term" value="C:respiratory chain complex III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006122">
        <property type="term" value="P:mitochondrial electron transport, ubiquinol to cytochrome c"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.210:FF:000001">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.210">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004205">
        <property type="entry name" value="Cyt_bc1_su8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036642">
        <property type="entry name" value="Cyt_bc1_su8_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12119:SF2">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12119">
        <property type="entry name" value="UBIQUINOL-CYTOCHROME C REDUCTASE COMPLEX UBIQUINONE-BINDING PROTEIN QP-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02939">
        <property type="entry name" value="UcrQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81508">
        <property type="entry name" value="Ubiquinone-binding protein QP-C of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0249">Electron transport</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 11" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR23974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="94" mass="10667" checksum="6E5E1AEF0EA102AB" modified="2009-07-28" version="1">MGGPTAKTFLGWWGSLGGPTQKGITSYAVSPYAQKPLAGIYHNAVFNTFRRVKAQALYLVIPAGLYWMWWVNCRDYNEYLYTKAGKEELDRVNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKJ2</accession>
    <name>C5DKJ2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Vacuolar protein sorting-associated protein 35</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0F05148g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Plays a role in vesicular protein sorting.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the VPS35 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554430.1">
        <property type="nucleotide sequence ID" value="XM_002554384.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKJ2"/>
    <dbReference type="FunCoup" id="C5DKJ2">
        <property type="interactions" value="1362"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKJ2"/>
    <dbReference type="GeneID" id="8292628"/>
    <dbReference type="KEGG" id="lth:KLTH0F05148g"/>
    <dbReference type="eggNOG" id="KOG1107">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005836_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKJ2"/>
    <dbReference type="OMA" id="YIRSREY"/>
    <dbReference type="OrthoDB" id="10258141at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030906">
        <property type="term" value="C:retromer, cargo-selective complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.660:FF:000007">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.660">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 35, helical subcomplex Vps35-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005378">
        <property type="entry name" value="Vps35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042491">
        <property type="entry name" value="Vps35_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11099:SF0">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11099">
        <property type="entry name" value="VACUOLAR SORTING PROTEIN 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03635">
        <property type="entry name" value="Vps35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009375">
        <property type="entry name" value="Retromer_Vps35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR009375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR23993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="857" mass="98894" checksum="4E83B899BB801287" modified="2009-07-28" version="1">MSYAENIEQAISNCRQQTVLMQRCLSQGKLMDALKHTSIMLTELRSPTLSPKQYYELYILIYDSLSILSSYFVECHPKKHHLADLYELVQYAGNILPRLYLMITVGSSYLQSSDAPREEILKDMIEMCKGVQNPIRGLFLRYYLSQRTKPFFETEDPEAKRANISLIVANFIEMNKLWVRLQHQGPLREREQRTRERKELKILVGTNLVRLSQIVESDFAMYRDEILPLVLEQVVQCRDIVSQEYLLDVICQVFPDEFHLGTLSELLSTTLKLSPAAPVNEVVLTLVERLNGYIDRQEHPGPDELAKKLESLDISSESQNVFFVFWKFLNQLNEERPDLSLQEFAPIIKGILKLTLRWYPDNLSNVDVLLKFMYEKCKESGQTVPEDYDYLFQELLLANDLRHDPRFFYQVLTKCESYQKLFRVQNKGLQKFCVNEILDAITHADLRISSETELQKILGVCEVMIEVGAEKASSSLILDEDDGTDAEKWSLNADQEKLAKIAHLCYNKSIEKHTELLIACREWFQKGDKNAKYLYPAVICNIWKLIRKCQLMIKRKPQRREKMSTTIKQLFKLASRTINELYNTCEGGCLDLLYKLNLQTASLADQLELGDIAYDFFSQAFTIFEESLSDSRTQFQAIVNMAQTLQKTRSLYAESSYDTLITRCTLHGSKLLKKQDQCRAVYTCSHLWWATEISLLGEEEGVTDNFFREGKRVLECLQRSLRVADSIMDNVQSCQLMVEILNRCCYYFVHGDENSTHVGTKYINGLIELIKTNLKSLKMEANQGEDSGERFFIGTDGSYIHQSPVNSVSAVYNKPPTSKGNNLASVPIEFFQRTLQYIEDQKDVDERFKAISVGSTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKS2</accession>
    <name>C5DKS2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="6">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ordered locus">KLTH0F07062g</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Lysosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="6"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554510.1">
        <property type="nucleotide sequence ID" value="XM_002554464.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKS2"/>
    <dbReference type="FunCoup" id="C5DKS2">
        <property type="interactions" value="29"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKS2"/>
    <dbReference type="GeneID" id="8292713"/>
    <dbReference type="KEGG" id="lth:KLTH0F07062g"/>
    <dbReference type="eggNOG" id="KOG1729">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069851_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKS2"/>
    <dbReference type="OMA" id="NFCPLHD"/>
    <dbReference type="OrthoDB" id="660555at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098588">
        <property type="term" value="C:bounding membrane of organelle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070936">
        <property type="term" value="P:protein K48-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16489">
        <property type="entry name" value="mRING-CH-C4HC2H_ZNRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051878">
        <property type="entry name" value="ZNRF_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46661">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE ZNRF1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46661:SF4">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0967">Endosome</keyword>
    <keyword evidence="16" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="15" id="KW-0458">Lysosome</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0519">Myristate</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="11 17" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FYVE-type" evidence="20">
        <location>
            <begin position="20"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="19">
        <location>
            <begin position="229"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="111"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="126"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="CAR24073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="287" mass="32407" checksum="2FAED890C553B056" modified="2009-07-28" version="1">MALIMPAPELVNSFAQWQADNLIHNCLNCQTKFSFLVRKHHCRCCGGIFCANCSDNFLWYDKRKVKVVRRKDDTDGFEVPPYRTCNSCCENLTQMHLIQPRWGDKLMRLQGSSMRTPQPQPAPVLPTSSSASSVQATELAKASGAAENTRDLDQESSRCPICNLDLFKLSEIDSAMHIQNCIERAESTQQHHDQSSPGESPAQRNRMLVYVIPPDQDTPDPTSQGDPECPICFEEMKPGDKVGRLECLCVFHYDCIKSWFRKKSQKLKSTTGKSVSKNFCPLHDAVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKS4</accession>
    <name>C5DKS4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">ATP-dependent rRNA helicase RRP3</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">ATP-dependent rRNA helicase rrp3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="ordered locus">KLTH0F07106g</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">ATP-dependent rRNA helicase required for pre-ribosomal RNA processing. Involved in the maturation of the 35S-pre-rRNA and to its cleavage to mature 18S rRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="13">Interacts with the SSU processome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the DEAD box helicase family. DDX47/RRP3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554512.1">
        <property type="nucleotide sequence ID" value="XM_002554466.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKS4"/>
    <dbReference type="FunCoup" id="C5DKS4">
        <property type="interactions" value="1224"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKS4"/>
    <dbReference type="GeneID" id="8292715"/>
    <dbReference type="KEGG" id="lth:KLTH0F07106g"/>
    <dbReference type="eggNOG" id="KOG0330">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_1_1_1"/>
    <dbReference type="InParanoid" id="C5DKS4"/>
    <dbReference type="OMA" id="GIGIKCC"/>
    <dbReference type="OrthoDB" id="10261904at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17954">
        <property type="entry name" value="DEADc_DDX47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000626">
        <property type="entry name" value="probable ATP-dependent RNA helicase DDX47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044765">
        <property type="entry name" value="DDX47/Rrp3_DEADc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF24">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 18" id="KW-0347">Helicase</keyword>
    <keyword evidence="6 18" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="22">
        <location>
            <begin position="105"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="20">
        <location>
            <begin position="136"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="21">
        <location>
            <begin position="339"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="22"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="501"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="17">
        <location>
            <begin position="105"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="40"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="82"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="515"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00024394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00024398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="CAR24075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="525" mass="58543" checksum="414A2B74EB5CBAFB" modified="2009-07-28" version="1">MGERREKFRRVKSMRCDELSLSAIEQRRSQQEAKMAGKVTKTEHKSSKQESSKGELKSLAEKIKKRALEKNQEASEKAPEGSEAEEEAEGVPNDSEQETEAAHFESFSELNLVPELIEACENLKYSKPTPIQAEAIPPALEGKDIIGLAQTGSGKTAAFAIPILNQLWHDQQPYYACVLAPTRELAQQIKETFDSLGGAMGVRTTCIVGGMNMIDQARDLMRKPHIIIATPGRLMDHLENTKGFSMRKLKFLVMDEADRLLDMEFGPVLDRILKIIPTQGRTTYLFSATMTSKIDKLQRASLTNPVKCAVSTKYQTVDTLIQTLMVVPGGLKNTFLIYLLNEFLGKTAIVFTRTKANAERIATLCNLLEFSATALHGDLNQNQRTGALDLFKAGRRSILVATDVAARGLDIPSVDIVINYDIPVDSKSYIHRVGRTARAGRSGKSISLVSQYDLELILRIEDVLGRKLPKENVDKGVILSLRDTVDKANGEVVMELNRRNKEKVARGKGRRSRMHSRENMDKEEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKT5</accession>
    <name>C5DKT5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0F07348p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0F07348g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554523.1">
        <property type="nucleotide sequence ID" value="XM_002554477.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKT5"/>
    <dbReference type="GeneID" id="8292726"/>
    <dbReference type="KEGG" id="lth:KLTH0F07348g"/>
    <dbReference type="HOGENOM" id="CLU_465441_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKT5"/>
    <dbReference type="OMA" id="CERHERL"/>
    <dbReference type="OrthoDB" id="4036003at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="341"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="378"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="554"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="171"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="181"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR24086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="586" mass="65699" checksum="7880CB2B0D7CF220" modified="2009-07-28" version="1">MMKYAKVHLEPPPVSAEAAAEHSREFSDSYLERRLRTRHSSKKSDSLFTRRALDYDGIKDSLLLYDDQHSDPRDTRAVKVPRRHMSGAKDCFECDSENPGCEVNFNRGKQPWYYNNSEDSRSIAADNLERPNTINSVRIKQVIENATHSIKNTQQACLGIRERLGKYQFTGQTPMPGAYTTDHEEQADTEHSPKTGLSRMTPEPAVRQVSEPIEYLPKAIIVPLLAKAAFFLLAQCATSEDIGYSFTGTCQLLISPESTTLTLPQLDALVLQLAGIWLATHSIPALTKAPWRFYQVAAQYWNSCLIGQDVPDLRRDGEFRELLELCERHERLSRRLRRTSLVVLLVSPVVLTLGLLLIHKLAFFWVTSNSTETAHRPVYSSLMAVAFSPAQFVILICCLWSQFFQDAFQHLELYVLAKHESFVQDTEFIADQLDRTCPSDTAGMDAGTKQSPLCNSPPRAAVKLEAIERSSATHPEGTLEPHAPLGPYALAAYESPLQHKEIAEPSFSAIRPVSPAAVVAPPEPLAATSEPSKAQRVLRLPFYVLSLYLKVLRLVFKIAIYAMLLSISLIIGPQNRRELSRPEKTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKZ1</accession>
    <name>C5DKZ1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0F08646p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0F08646g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554579.1">
        <property type="nucleotide sequence ID" value="XM_002554533.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKZ1"/>
    <dbReference type="FunCoup" id="C5DKZ1">
        <property type="interactions" value="77"/>
    </dbReference>
    <dbReference type="GeneID" id="8292785"/>
    <dbReference type="KEGG" id="lth:KLTH0F08646g"/>
    <dbReference type="eggNOG" id="ENOG502QRGG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018066_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKZ1"/>
    <dbReference type="OMA" id="NIYIHDG"/>
    <dbReference type="OrthoDB" id="4070605at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="278"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="334"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="467"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="605"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="336"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="360"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="400"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="433"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="628"/>
            <end position="656"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="715" mass="78804" checksum="F2D43177F84D203F" modified="2009-07-28" version="1">MKEPTSGRSPASQVLTAKALDSTPQPPKDGPKVKVKRLDSLFLISYSDASARSPATLLAVHDFRVSLYIDIPTIDYYNDQLRHNVISRINKKFKVHKLKEQIFASITNFDERTWDLILNNGYPQSPVQFNFSVSSSGSLRYVETIKFLVESCWDKIKSSSDRVHAVFKLQVSPTSLIWFKTFLNKDLFACAAVQFEVIGNYNMISSSTTPFKEYYAKLNDKFTKKTSSTDFDSIIVVTNSTGVKALLTILSDKPLTSYLSKDSLEALHSNALLKQPSKPIAAENADSDDETPLKRDSSSLLSFQNSLITSNKDKSVKVRTPFIGRVPYNRRADAKNTFQFATDNPSTDEDSETQPLETLEPFDDSDLDEEEDDEGDDDEDEDGLSFSVPSRLSRCGSETDCASQTPQEGSTSRRFRSLSLMDPALQAPFAQRSEPSGSRVPSSIENFGDETCAPTAKLLNQGPTNIYVHDGQFGEGPAPGPIRRPRRMPRTKSANNVSIGLIPPEFFSRISSPSSSNSSSNTSLTNLNILPGTFSKLLEPSGDGGDEESQSLFKRSLIDKSFKEVKKQPSFHFGSNPLAAHDLNRFALNFRPSKTIPISAKVLDDEDRLMSGQSNFNEQTSDEEARSNADSTSTLVRPGSLKSNSSANSPGLQNLKLQIYGDNENPSGNENGAQEKGPEPAKPKPAYKKPKFTLDLYNDDEVQSSGGWLLGGNAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DL04</accession>
    <name>C5DL04_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Succinate dehydrogenase [ubiquinone] cytochrome b small subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0F08932g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Mitochondrion inner membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the CybS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554592.1">
        <property type="nucleotide sequence ID" value="XM_002554546.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DL04"/>
    <dbReference type="FunCoup" id="C5DL04">
        <property type="interactions" value="284"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DL04"/>
    <dbReference type="GeneID" id="8292798"/>
    <dbReference type="KEGG" id="lth:KLTH0F08932g"/>
    <dbReference type="eggNOG" id="KOG4097">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096618_0_0_1"/>
    <dbReference type="InParanoid" id="C5DL04"/>
    <dbReference type="OMA" id="HSCITDY"/>
    <dbReference type="OrthoDB" id="18577at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098796">
        <property type="term" value="C:membrane protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048039">
        <property type="term" value="F:ubiquinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006121">
        <property type="term" value="P:mitochondrial electron transport, succinate to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03496">
        <property type="entry name" value="SQR_TypeC_CybS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1300.10:FF:000007">
        <property type="entry name" value="Succinate dehydrogenase [ubiquinone] cytochrome b small subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1300.10">
        <property type="entry name" value="Fumarate reductase/succinate dehydrogenase, transmembrane subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007992">
        <property type="entry name" value="CybS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034804">
        <property type="entry name" value="SQR/QFR_C/D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13337:SF5">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE PROTEIN SHH4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13337">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05328">
        <property type="entry name" value="CybS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81343">
        <property type="entry name" value="Fumarate reductase respiratory complex transmembrane subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 11" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="128"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="10">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <note>ligand shared with SDHC</note>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>a ubiquinone</name>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <note>ligand shared with IP/SDHB</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR607992-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR607992-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU364031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR24155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="170" mass="18174" checksum="2561CAD33F899242" modified="2009-07-28" version="1">MFSRGLMARSAAMSAAMRALHSTSKRSLTIPFLPTLPPTPGGVKGDVNDPTYTPPPDKLHGSMHWDFERFTAVALVPLVGSGLACGAGLSVVADSLLCSVLLAHGYVGFQSCIIDYIPKRVYGKNHNYAMYLLTLGSLVGGFGIYKLETEENGITGLVKALWNAGKEDKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5DL26</accession>
    <name>C5DL26_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0F09438p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0F09438g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554614.1">
        <property type="nucleotide sequence ID" value="XM_002554568.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DL26"/>
    <dbReference type="FunCoup" id="C5DL26">
        <property type="interactions" value="160"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DL26"/>
    <dbReference type="GeneID" id="8292821"/>
    <dbReference type="KEGG" id="lth:KLTH0F09438g"/>
    <dbReference type="eggNOG" id="KOG2218">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_410507_0_0_1"/>
    <dbReference type="InParanoid" id="C5DL26"/>
    <dbReference type="OMA" id="TCSQIFM"/>
    <dbReference type="OrthoDB" id="2189254at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070939">
        <property type="term" value="C:Dsl1/NZR complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.210">
        <property type="entry name" value="Dsl1p vesicle tethering complex, Tip20p subunit, domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1420">
        <property type="entry name" value="Dsl1p vesicle tethering complex, Tip20p subunit, domain B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.100">
        <property type="entry name" value="Dsl1p vesicle tethering complex, Tip20p subunit, domain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.670">
        <property type="entry name" value="Dsl1p vesicle tethering complex, Tip20p subunit, domain D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2270">
        <property type="entry name" value="Dsl1p vesicle tethering complex, Tip20p subunit, domain E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042044">
        <property type="entry name" value="EXOC6PINT-1/Sec15/Tip20_C_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007528">
        <property type="entry name" value="RINT1_Tip20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042041">
        <property type="entry name" value="Tip20p_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042042">
        <property type="entry name" value="Tip20p_domB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042043">
        <property type="entry name" value="Tip20p_domC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042040">
        <property type="entry name" value="Tip20p_domE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04437">
        <property type="entry name" value="RINT1_TIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51386">
        <property type="entry name" value="RINT1_TIP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR24177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="675" mass="77739" checksum="3CCA17C2E21859F0" modified="2009-07-28" version="1">MLQSIEDLKGIPQLIHEVEAQRGELAEKIQGHNSPGDDLLDDFDMLDAQLAKAAADVVQARSTADVKALKEKYGNLAILEQLDSLFTREETKQAQKHALVKVQNIYDSMAAQQEANSAQLELWHEDIKQALAEVDPDLGAQSGVYVRFTALLTQEAAVLSEELKTLLLNANWDATQFSVVDAVATEHLRDLSNRLYRLWQLQLPEPDSNSIWNFECIANNFKIKFIYHFNNQAVQEPQSVEMYFKFLDKYLDQNLFKCVDIFQDPSSSSSMTQELVHQQFINHILNPIREKVSSTLLKNPEISSDQDLKTLIALISQIFIIDNALIKKHLYYGHGLIAMIPKSILDTWHQFEIEASISQFQKIIKSPLEKSGVDFCKLLQNLYAYFEPFFHIDFDELMDYKIKITNRIFMGLPNRYRENLLAVKDDQATSYTDEAQFKQTLWKLNNLELIMSLLKDLETKLTFIQITNYVNIATSSDYSTVLSDVLDSYEDTVIAVKDSVIHRIKKMLNMSLRNYFKLNEWALVTSEPHQCSAELIAPLSLSNRLIGTMDSFDLKPHTTLSVKNEVLNIIIHYMLDYVVNLNKFSEMGFVQLQLDYNALKETLSFPHVSIPQNAEEGAFLESLKVLELRYKRSQTNKQFLSKSYIDRREFSAFRDYLQIKYSTDSELANAMYRLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DL65</accession>
    <name>C5DL65_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0F10362p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0F10362g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554653.1">
        <property type="nucleotide sequence ID" value="XM_002554607.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DL65"/>
    <dbReference type="FunCoup" id="C5DL65">
        <property type="interactions" value="698"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DL65"/>
    <dbReference type="GeneID" id="8292863"/>
    <dbReference type="KEGG" id="lth:KLTH0F10362g"/>
    <dbReference type="eggNOG" id="ENOG502QTE4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068713_0_0_1"/>
    <dbReference type="InParanoid" id="C5DL65"/>
    <dbReference type="OMA" id="FFSKRMN"/>
    <dbReference type="OrthoDB" id="439808at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050374">
        <property type="entry name" value="RRT5_SRSF_SR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003">
        <property type="entry name" value="RNA RECOGNITION MOTIF RRM DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003:SF62">
        <property type="entry name" value="SERINE_ARGININE (SR)-TYPE SHUTTLING MRNA BINDING PROTEIN NPL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="51"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="210"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="127"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="296"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="8"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="127"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="145"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="299"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="313"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="328"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR24216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="347" mass="37452" checksum="498F83EBFF690BBB" modified="2009-07-28" version="1">MSATTENAASPVEAPVEAPVDASAGAATGTAAAAPVESGSAAPRSKVDPETTIFIGNVARDCQEEDLKKVFEEDVEVEIPSVKSGRLFKQRYAFVKFPHKIDFDAVKAKYDKTVIKERGIYIRRAQTEEERDAKRKARAERPRKAAAATTGGSAEAETPASAGEAATDAADAASGVSGAGKKPARSAVPAPPKRQERPRVPLETMERSTDTLYVNNVPYFATKEELAEFFGTTPELVVLPLRRMRNVKTKQFFYSKRMNRGIAFVTFENCADIAQKVQEFQGKSLNDRELAVDVAALKPPREDQKPQDAEQPAQATEPEQSTQSTQSAEPAKPAEPVAASQQDSPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5DL72</accession>
    <name>C5DL72_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0F10560p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0F10560g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the spermidine/spermine synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554660.1">
        <property type="nucleotide sequence ID" value="XM_002554614.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DL72"/>
    <dbReference type="FunCoup" id="C5DL72">
        <property type="interactions" value="1061"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DL72"/>
    <dbReference type="GeneID" id="8292872"/>
    <dbReference type="KEGG" id="lth:KLTH0F10560g"/>
    <dbReference type="eggNOG" id="KOG1562">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048199_1_0_1"/>
    <dbReference type="InParanoid" id="C5DL72"/>
    <dbReference type="OMA" id="FLYHEMM"/>
    <dbReference type="OrthoDB" id="38125at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004766">
        <property type="term" value="F:spermidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015940">
        <property type="term" value="P:pantothenate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008295">
        <property type="term" value="P:spermidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.140.10:FF:000001">
        <property type="entry name" value="SPE3p Spermidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000013">
        <property type="entry name" value="Spermidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.140.10">
        <property type="entry name" value="Spermidine synthase, tetramerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00198">
        <property type="entry name" value="Spermidine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030374">
        <property type="entry name" value="PABS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030373">
        <property type="entry name" value="PABS_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001045">
        <property type="entry name" value="Spermi_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030668">
        <property type="entry name" value="Spermi_synthase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035246">
        <property type="entry name" value="Spermidine_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037163">
        <property type="entry name" value="Spermidine_synt_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002010">
        <property type="entry name" value="PRK00811.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00417">
        <property type="entry name" value="speE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11558:SF11">
        <property type="entry name" value="SPERMIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11558">
        <property type="entry name" value="SPERMIDINE/SPERMINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17284">
        <property type="entry name" value="Spermine_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01564">
        <property type="entry name" value="Spermine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000502">
        <property type="entry name" value="Spermidine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01330">
        <property type="entry name" value="PABS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51006">
        <property type="entry name" value="PABS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PABS" evidence="5">
        <location>
            <begin position="12"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR24223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="294" mass="33419" checksum="247494CAFC090AEE" modified="2009-07-28" version="1">MSQEITHPTIKDGWFREISDTMWPGQAMTLRVEKVLHHEKSKYQDVLIFKSTDYGNVLVLDNAIQVTERDEFSYQEMIAHLGLNSHPNPKKVLVIGGGDGGVLREIVKHESVEEAWLCDIDEAVIRLSKQYLPEMAASYSHPKVKTHIGDGFQFLRDYQNTFDVIITDSSDPEGPAETLFQKPYFELLSSALTEKGVITTQGESIWLHLPIIKELKKACSEVFPTVEYAYTTIPTYPSGQIGFMVCSKDGSVDVKKPLRQKSDEEEAKLYKYYNKKIHEAAFVLPTWAAKELEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5DL88</accession>
    <name>C5DL88_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">KLTH0F10912p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0F10912g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Tom20 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554676.1">
        <property type="nucleotide sequence ID" value="XM_002554630.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DL88"/>
    <dbReference type="FunCoup" id="C5DL88">
        <property type="interactions" value="356"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DL88"/>
    <dbReference type="GeneID" id="8292888"/>
    <dbReference type="KEGG" id="lth:KLTH0F10912g"/>
    <dbReference type="eggNOG" id="KOG4056">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090411_0_0_1"/>
    <dbReference type="InParanoid" id="C5DL88"/>
    <dbReference type="OMA" id="PPPIFQI"/>
    <dbReference type="OrthoDB" id="2154253at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005742">
        <property type="term" value="C:mitochondrial outer membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030943">
        <property type="term" value="F:mitochondrion targeting sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016031">
        <property type="term" value="P:tRNA import into mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.960.10">
        <property type="entry name" value="Mitochondrial outer membrane translocase complex, subunit Tom20 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002056">
        <property type="entry name" value="MAS20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023392">
        <property type="entry name" value="Tom20_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12430">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12430:SF0">
        <property type="entry name" value="TRANSLOCASE OF OUTER MITOCHONDRIAL MEMBRANE 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02064">
        <property type="entry name" value="MAS20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037707">
        <property type="entry name" value="MAS20_rcpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00351">
        <property type="entry name" value="OM20RECEPTOR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47157">
        <property type="entry name" value="Mitochondrial import receptor subunit Tom20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="41"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR24239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="194" mass="20817" checksum="8160D78EBBCBA322" modified="2009-07-28" version="1">MSQQPGSIARILAVTGLTATVAIAGYAAYFDYQRRNNPDFRKSLKRKLKKHAEEEKQAKERAKQQKLAHVGEYLTAELAKDPVSTDPTQREAVFTSNVELGERLASVAGNELESALKFYKALSVYPNPADLLGIYQRSVPEAVYEYIVLMIAIMPPVNVSTFLSGGAQGGPAAVAQAMAGQAGNTGAGVGEIDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLD9</accession>
    <name>C5DLD9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0F12232p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0F12232g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Component of the 19S cap proteasome complex which acts as a regulatory subunit of the 26S proteasome, involved in the ATP-dependent degradation of ubiquitinated proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">The 26S proteasome is composed of a core protease, known as the 20S proteasome, capped at one or both ends by the 19S regulatory complex (RC). The RC is composed of at least 18 different subunits in two subcomplexes, the base and the lid, which form the portions proximal and distal to the 20S proteolytic core, respectively. Component of the lid subcomplex of the 19S RC.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554727.1">
        <property type="nucleotide sequence ID" value="XM_002554681.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLD9"/>
    <dbReference type="FunCoup" id="C5DLD9">
        <property type="interactions" value="1164"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLD9"/>
    <dbReference type="GeneID" id="8292944"/>
    <dbReference type="KEGG" id="lth:KLTH0F12232g"/>
    <dbReference type="eggNOG" id="KOG0687">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031814_1_1_1"/>
    <dbReference type="InParanoid" id="C5DLD9"/>
    <dbReference type="OMA" id="RLHCKVD"/>
    <dbReference type="OrthoDB" id="1452at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.570:FF:000005">
        <property type="entry name" value="26S proteasome regulatory subunit N7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019585">
        <property type="entry name" value="Rpn7/CSN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045135">
        <property type="entry name" value="Rpn7_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049549">
        <property type="entry name" value="RPN7_PSMD6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14145:SF1">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14145">
        <property type="entry name" value="26S PROTESOME SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10602">
        <property type="entry name" value="RPN7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21154">
        <property type="entry name" value="RPN7_PSMD6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0647">Proteasome</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="4">
        <location>
            <begin position="109"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="PCI" evidence="6">
        <location>
            <begin position="201"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="81"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00093435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00093502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR24290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="408" mass="46751" checksum="5A082B33ED401BA2" modified="2009-07-28" version="1">MAEEESNYVESSVANVPNYEVADHAFILSEPRLKDQHEASLKFVLDKVKEEAMAPFYKFLYEEYLPKGAFEWDETLYKSLVDRNQQNITELKERLQEVEEKDEGELEKAQAWVKLGEYYAQIGDKTNAEKTLAKALDKAVSTGSKIDIMLTITRLGFFYNDQQFVKEKLEQTFSMIEKGGDWERRNRYKTYKGVHCLAIRDFKEAANLLVDSLATFTSTELTSYENIATYASVAGLFALERTELKDKIIDSPELLSLMTNTPALQSISSLTISLYTSEYSSYFPFLLETYDKVLVPCKYLSKHADFFVREMRRKVYAQLLESYKTLSIKTMASAFGVSVDFLDKDLGKFIPNKQLNCVIDRVNGIVETNRPDNKNVQYHTLIKQGDGLLTKLQKYGTAVRLTGTDRVV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-05-31" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLJ3</accession>
    <name>SHE10_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Outer spore wall assembly protein SHE10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Sensitivity to high expression protein 10</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">SHE10</name>
        <name type="ordered locus">KLTH0G01166g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in spore wall assembly. May be a component of the mitochondrial RNase MRP (MtMRP), a ribonucleoprotein endoribonuclease involved in the cleaving RNA transcripts to generate primers for DNA replication in mitochondria.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the mitochondria-localized RNase mitochondrial RNA-processing (RNase MRP) composed of one single RNA encoded by the NME1 gene and at least 31 proteins. Absent in the nucleus-localized RNase MRP (NuMRP).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SHE10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555091.1">
        <property type="nucleotide sequence ID" value="XM_002555045.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLJ3"/>
    <dbReference type="SMR" id="C5DLJ3"/>
    <dbReference type="FunCoup" id="C5DLJ3">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLJ3"/>
    <dbReference type="GeneID" id="8293349"/>
    <dbReference type="KEGG" id="lth:KLTH0G01166g"/>
    <dbReference type="eggNOG" id="ENOG502QT2T">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023952_1_0_1"/>
    <dbReference type="InParanoid" id="C5DLJ3"/>
    <dbReference type="OMA" id="DEFQAWS"/>
    <dbReference type="OrthoDB" id="3260408at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030435">
        <property type="term" value="P:sporulation resulting in formation of a cellular spore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0175">Coiled coil</keyword>
    <keyword id="KW-0496">Mitochondrion</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0749">Sporulation</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000408909" description="Outer spore wall assembly protein SHE10">
        <location>
            <begin position="25"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="530"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="587"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="503"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="617"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="593"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="612"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="630"/>
            <end position="673"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P53075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="673" mass="78644" checksum="490F6314EE816AE8" modified="2009-07-28" version="1" precursor="true">MRRVRGVGNLLVTILVVLIGFKQARKYCESNEAEFCRLTSLSRASPWWNEHIDPYWDSYVSPCVTKIHDYEHKLDEWAAPRIDRAYYQVQDIVETKVVPVTYKWYKTVQFKTQVYYNGHFVPFLGHARILAKQWLASEGFFQRLYLHVQQGLFCLRNWSLWVWNGSLEAFNEIHSWSGHALQEISNKFSVTENQSGEPVTDEDPSDTDESKYYDAEYFEGEQGDYNEDDETVYLTSTIIETVTLSDEKQLDKSTSAANGDDGSSLDVPLRDLVQDEFQAWSNTVEQKASNTLTQFDSEVEELVEAKLSKAQPNITGLLQGISESFQSHYRIINRAILDVDCTMELDPETGEQLYFNKDGTQLRKYVTRPLMREFFSSAHAHVDERLEMVRARLEKFVAAVNKEVEQVRQEHLEVYEEWGDVMVSEWSKRMAYVDVVAAMGAEDMNQQQHDNWKRFLKLKKQVVNTRDLLMQHPAKLERLQKFLNDIQFTLMALQRDAGEYLFILRSQANIAFQTREKLEREREEEERRKLEQERQIQEQKEREQQELAENEARAARERLALEQGQGQEQLEQNSDEAFFSMEDITATPLGADDNTDYENALDDADSQLSESGDSEDNFYDSYEGDEEDASRELERLERESAERETLDRLELGQRQKLQEEQHRDELHHSSQAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLJ7</accession>
    <name>C5DLJ7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0G01254p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0G01254g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555095.1">
        <property type="nucleotide sequence ID" value="XM_002555049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLJ7"/>
    <dbReference type="FunCoup" id="C5DLJ7">
        <property type="interactions" value="23"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLJ7"/>
    <dbReference type="GeneID" id="8293353"/>
    <dbReference type="KEGG" id="lth:KLTH0G01254g"/>
    <dbReference type="eggNOG" id="KOG4075">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148802_0_0_1"/>
    <dbReference type="InParanoid" id="C5DLJ7"/>
    <dbReference type="OMA" id="YLDWKME"/>
    <dbReference type="OrthoDB" id="186013at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.442.10">
        <property type="entry name" value="Cytochrome c oxidase subunit IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004203">
        <property type="entry name" value="Cyt_c_oxidase_su4_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036639">
        <property type="entry name" value="Cyt_c_oxidase_su4_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10707">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10707:SF9">
        <property type="entry name" value="MAINTENANCE OF TELOMERE CAPPING PROTEIN 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02936">
        <property type="entry name" value="COX4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81406">
        <property type="entry name" value="Mitochondrial cytochrome c oxidase subunit IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR24658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="149" mass="17188" checksum="3486F563B648D321" modified="2009-07-28" version="1">MSTSLRSLRRNLGCLRRGTSHVPACLRSLSHTSYEPPRIDDLTGEKWIKLEKDVKEEIMEYLDWKMEGDWREMPANEKRASYFVSFGQWGPRAKPGSKEAQLQMTGAEIILRGVFSGVLFMAVAVSFMNYQNDKRVQKNLKKLEDSAER</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLP4</accession>
    <name>C5DLP4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0G02376p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0G02376g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The proteasome degrades poly-ubiquitinated proteins in the cytoplasm and in the nucleus. It is essential for the regulated turnover of proteins and for the removal of misfolded proteins. The proteasome is a multicatalytic proteinase complex that is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. It has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the peptidase T1A family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555142.1">
        <property type="nucleotide sequence ID" value="XM_002555096.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLP4"/>
    <dbReference type="FunCoup" id="C5DLP4">
        <property type="interactions" value="976"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLP4"/>
    <dbReference type="MEROPS" id="T01.973"/>
    <dbReference type="GeneID" id="8293404"/>
    <dbReference type="KEGG" id="lth:KLTH0G02376g"/>
    <dbReference type="eggNOG" id="KOG0178">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035750_4_3_1"/>
    <dbReference type="InParanoid" id="C5DLP4"/>
    <dbReference type="OMA" id="YVLNDNM"/>
    <dbReference type="OrthoDB" id="431557at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010499">
        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03752">
        <property type="entry name" value="proteasome_alpha_type_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000009">
        <property type="entry name" value="Proteasome subunit alpha type-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003075">
        <property type="entry name" value="PRK03996.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 8" id="KW-0647">Proteasome</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="9">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00026071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR24705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="251" mass="27721" checksum="BC416B23E12224FE" modified="2009-07-28" version="1">MGSRRFDSRTTIFSPEGRLYQVEYALESISHAGTAIGVMAKDGIVLAAERKVTSKLLEQGSSSEKLYKLNDNITVAVAGLTADAEILINTARLFAQNYLKSYNEEIPVEILVRRLSDVKQGYTQHGGLRPFGASFIYAGYDDRYGYQLYTSNPSGNYSGWKAISVGANTSAAQTLLQMDYKDDITVEEATELALKTLSKTTDSSTLTPERVEFSTITKLDNGKIAQKIFKPQEIETLLNKTGVTKKENDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLR0</accession>
    <name>C5DLR0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0G02750p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0G02750g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555158.1">
        <property type="nucleotide sequence ID" value="XM_002555112.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLR0"/>
    <dbReference type="GeneID" id="8293421"/>
    <dbReference type="KEGG" id="lth:KLTH0G02750g"/>
    <dbReference type="HOGENOM" id="CLU_1731795_0_0_1"/>
    <dbReference type="InParanoid" id="C5DLR0"/>
    <dbReference type="OrthoDB" id="10575662at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR24721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="151" mass="16935" checksum="BBBA2726925B7F91" modified="2009-07-28" version="1">MLRDMLRARPNAVCAWGGSLFFAAAHRSARKFWLVRRRSRTPVETHTLGKAQRPGSGAGLCQRCATRMSIHTGGRSSMREWRESLVAKAPVAPRLCSATRFPASWIRPLDVRLSLGAPLACCRTSLCTTEPSPPHRKIFQNFNAMQFENLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLR4</accession>
    <name>C5DLR4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0G02838p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0G02838g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555162.1">
        <property type="nucleotide sequence ID" value="XM_002555116.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLR4"/>
    <dbReference type="GeneID" id="8293425"/>
    <dbReference type="KEGG" id="lth:KLTH0G02838g"/>
    <dbReference type="HOGENOM" id="CLU_1731796_0_0_1"/>
    <dbReference type="InParanoid" id="C5DLR4"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="151" mass="16783" checksum="3FB5CDAE7CA95896" modified="2009-07-28" version="1">MTKHASLCINAKNTAISNGSPQATHWKPQPFKGIQVQTLLACMLDSGCRILHRAAQMFHIRFASILSASSTLFTILLPARPANASSLSGFRIPHAAVTLPVGHFWLPVSLLCPFSFDLYAPMANFRKENLCILLDQVTERVKYFIRHVACF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLV8</accession>
    <name>C5DLV8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0G03916p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0G03916g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the proteasome subunit S11 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555206.1">
        <property type="nucleotide sequence ID" value="XM_002555160.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLV8"/>
    <dbReference type="FunCoup" id="C5DLV8">
        <property type="interactions" value="1445"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLV8"/>
    <dbReference type="GeneID" id="8293472"/>
    <dbReference type="KEGG" id="lth:KLTH0G03916g"/>
    <dbReference type="eggNOG" id="KOG2908">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042989_0_0_1"/>
    <dbReference type="InParanoid" id="C5DLV8"/>
    <dbReference type="OMA" id="SFEDYWE"/>
    <dbReference type="OrthoDB" id="1093at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054179">
        <property type="entry name" value="PSD13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035298">
        <property type="entry name" value="PSMD13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040798">
        <property type="entry name" value="Rpn9_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10539">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10539:SF0">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22037">
        <property type="entry name" value="PSD13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18261">
        <property type="entry name" value="Rpn9_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0647">Proteasome</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="3">
        <location>
            <begin position="185"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR24769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="394" mass="45621" checksum="7F539444AF4DE361" modified="2009-07-28" version="1">MSQDIDTILSTLRTEADPELAGLFYEFEDFYEQKLWHQLTERLDVFFHDERSLPLRLRVYDTFVSKFQDKINQLKLTNFLLLSLKDNSESQDALDYLTDLQKSFKEMDEKKQRNDGLKSHYSGILLIDIEIARIYLVQGDLVKARDSLDEIETTLEFQDSLPLQVISAFYSTSAEYYLAKKDFNSFYYTSLLYLSTAETLKSYHELSPVKRQQLAYNLCVAALLGDKIYNLGELLNHPIMESISSDPQYEWLSQFLQTLSNGDFDQFDKLSKERVPHVPILSAHESFLRQKICLMTLVESVFVKNIRTLSFDDIAAATHLPKDNVEHLVMRSISLGLLKGSIDQVNELVTVAWVQPRIINGDQINKMKQRLVEWNEEVSSLGKKIEARGKSIWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>C5DM86</accession>
    <name>C5DM86_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0G06820p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0G06820g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RCAN family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555334.1">
        <property type="nucleotide sequence ID" value="XM_002555288.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DM86"/>
    <dbReference type="FunCoup" id="C5DM86">
        <property type="interactions" value="42"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DM86"/>
    <dbReference type="GeneID" id="8293603"/>
    <dbReference type="KEGG" id="lth:KLTH0G06820g"/>
    <dbReference type="eggNOG" id="KOG4019">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1390040_0_0_1"/>
    <dbReference type="InParanoid" id="C5DM86"/>
    <dbReference type="OMA" id="MPPRSIF"/>
    <dbReference type="OrthoDB" id="17212at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008597">
        <property type="term" value="F:calcium-dependent protein serine/threonine phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006931">
        <property type="entry name" value="Calcipressin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10300">
        <property type="entry name" value="CALCIPRESSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10300:SF14">
        <property type="entry name" value="PROTEIN SARAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04847">
        <property type="entry name" value="Calcipressin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="106"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="180"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="121"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR24897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="210" mass="23125" checksum="B70B7BE97F4DE78E" modified="2009-07-28" version="1">MATNTLIITSDKKDVTQGETADQVRKFLESQVLTRYSVTRADAMQLVALPSLQRLILVCPSVEIAQEAMTAKARHPDLESLQFRFSVADTTLDSRKQYLKLPPKQSMFLVSPPTSPPPEFDYSRLEETPNREKGLAPVSHHSQTSAAQPAEQALLSTGHYVLLETPRASITLDCCTRDTDAGDEPATDHVSVSGVRTGMPPRSIFDDVED</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMK4</accession>
    <name>C5DMK4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0G09658p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0G09658g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic mitochondrial porin family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555452.1">
        <property type="nucleotide sequence ID" value="XM_002555406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMK4"/>
    <dbReference type="FunCoup" id="C5DMK4">
        <property type="interactions" value="1137"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMK4"/>
    <dbReference type="GeneID" id="8293730"/>
    <dbReference type="KEGG" id="lth:KLTH0G09658g"/>
    <dbReference type="eggNOG" id="KOG3126">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044399_0_1_1"/>
    <dbReference type="InParanoid" id="C5DMK4"/>
    <dbReference type="OMA" id="KPCCSHE"/>
    <dbReference type="OrthoDB" id="7827681at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046930">
        <property type="term" value="C:pore complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015288">
        <property type="term" value="F:porin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008308">
        <property type="term" value="F:voltage-gated monoatomic anion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07306">
        <property type="entry name" value="Porin3_VDAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.10">
        <property type="entry name" value="Porin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023614">
        <property type="entry name" value="Porin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001925">
        <property type="entry name" value="Porin_Euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027246">
        <property type="entry name" value="Porin_Euk/Tom40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11743:SF70">
        <property type="entry name" value="GH26960P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11743">
        <property type="entry name" value="VOLTAGE-DEPENDENT ANION-SELECTIVE CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01459">
        <property type="entry name" value="Porin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00185">
        <property type="entry name" value="EUKARYTPORIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00558">
        <property type="entry name" value="EUKARYOTIC_PORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0626">Porin</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR25015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="281" mass="29035" checksum="CD35BB382C98DA8D" modified="2009-07-28" version="1">MAPPFFSDISKDINGLLNRDFYHGTPAAVDIKTTAPNGVAFAVKGKSSPKDNAIAANVEAKVTDKATGLTLTQGWSSANALDTKIELAELTPGLKSELATTFVPGASKAAKLNLSFIQPFFTARGAFDLLKGPSFVGDLTLAHEGFVGGAEVGYDIAAAAVSRYAVALGYSAGAYSVALSINNAQLTTASFFQKVSPLLDVGAKAVLNPQAGSNVNIEFASKYTLDATSQVKAKVADSGIVALAYKQALRPGVDLGVGASFDALKLSEPVHKLGWSLSFSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMQ5</accession>
    <name>C5DMQ5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">KLTH0G10802p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0G10802g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555503.1">
        <property type="nucleotide sequence ID" value="XM_002555457.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMQ5"/>
    <dbReference type="FunCoup" id="C5DMQ5">
        <property type="interactions" value="844"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMQ5"/>
    <dbReference type="GeneID" id="8293781"/>
    <dbReference type="KEGG" id="lth:KLTH0G10802g"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030450_0_0_1"/>
    <dbReference type="InParanoid" id="C5DMQ5"/>
    <dbReference type="OMA" id="HEQMRQG"/>
    <dbReference type="OrthoDB" id="8117402at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071277">
        <property type="term" value="P:cellular response to calcium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071467">
        <property type="term" value="P:cellular response to pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000065">
        <property type="entry name" value="B-cell CLL/lymphoma 6, member B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000181">
        <property type="entry name" value="C2H2 type zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000239">
        <property type="entry name" value="C2H2 type zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF120">
        <property type="entry name" value="KRUPPEL-LIKE FACTOR 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="408"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="436"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="464"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="268"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="319"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="363"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="28"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="271"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="376"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR25066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="526" mass="57858" checksum="101D2D40E28E76CB" modified="2009-07-28" version="1">MDFNQFMSYQEKSGEKSNTNSPKDTPQGGSGLFPGSGGREEPAQLTLNTGFNGWAGTSRHVSEAESIFLSPLSEDSPRGFPVGQRLATPTINIEQTQEPFDENDAMRAFVGNIQEPFDSLGLGFQNELQGAPSSFSPAHSMLSPHFNDDFVSTRSASVHSATSDSGRTAGAHRVDIALSPVASRLTTGDDELDEILSVRSGPDPFGDIDYPQESQSIPAQDVSSFLSPVDNMFGFGDSSAYSDPAPLPANFSRPQISVQEFQVITPAEMSKSPQVSASPSPSNSFQGGPSQQNTNLLSVERDDVAVTSDDEEVHDLMRQGRKVRRKSAQGFVRSPSRGRSLSVDEKARSLSENRERLLELAALKPSKSHNDRSEEQSDGYDFTEEDSLPMNVSGVTRRKSIQKNPAVYACELCDKKFTRPYNLKSHLRTHTDERPFACAICGKAFARQHDRKRHEDLHTGKKRYTCKGKLKDGTQWGCGKKFARSDALGRHFKTEGGKRCIAPLYEEVTREREAGRKPEIDLPIDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMS8</accession>
    <name>C5DMS8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Hsp90 chaperone protein kinase-targeting subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0G11330g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CDC37 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555526.1">
        <property type="nucleotide sequence ID" value="XM_002555480.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMS8"/>
    <dbReference type="FunCoup" id="C5DMS8">
        <property type="interactions" value="476"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMS8"/>
    <dbReference type="GeneID" id="8293805"/>
    <dbReference type="KEGG" id="lth:KLTH0G11330g"/>
    <dbReference type="eggNOG" id="KOG2260">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033261_1_0_1"/>
    <dbReference type="InParanoid" id="C5DMS8"/>
    <dbReference type="OMA" id="NYSKWDQ"/>
    <dbReference type="OrthoDB" id="440202at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.610">
        <property type="entry name" value="Cdc37, Hsp90 binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004918">
        <property type="entry name" value="Cdc37"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013873">
        <property type="entry name" value="Cdc37_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013874">
        <property type="entry name" value="Cdc37_Hsp90-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038189">
        <property type="entry name" value="Cdc37_Hsp90-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013855">
        <property type="entry name" value="Cdc37_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12800">
        <property type="entry name" value="CDC37-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12800:SF4">
        <property type="entry name" value="HSP90 CO-CHAPERONE CDC37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08564">
        <property type="entry name" value="CDC37_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08565">
        <property type="entry name" value="CDC37_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03234">
        <property type="entry name" value="CDC37_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01069">
        <property type="entry name" value="CDC37_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01070">
        <property type="entry name" value="CDC37_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01071">
        <property type="entry name" value="CDC37_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101391">
        <property type="entry name" value="Hsp90 co-chaperone CDC37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cdc37 N-terminal" evidence="9">
        <location>
            <begin position="2"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="Cdc37 Hsp90 binding" evidence="8">
        <location>
            <begin position="186"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="Cdc37 C-terminal" evidence="7">
        <location>
            <begin position="379"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="460"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="470"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="484"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00031396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR25089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="501" mass="57153" checksum="EB6BE74141AC7C3A" modified="2009-07-28" version="1">MPIDYSKWDKIELSDDSDIEVHPNVDKRSFIRWKQQSIHEERAKRNNDIKNLEFQVNMNGRLNQRVDKMLANLKDADLVDREAVRKFLNANFDKTEKGEEDNVDPNMPPYNEMVEDLFEQLEINAKKVGANPKDGAVIRKLILEHRQKIDAVTKQAKEKLSQLYKEKASLISSDDIRTGFDKGFVNKKAEAENAAAKALTSTQSSAPSEPSLPAPPLNFVSFEDDVMELAPQTKAFGQISPDNIQGSESFLLKNLQIMSEQQKDALMMSAFEFEMEGYSKQAYQVIHQSELISYIREIYDMKKIPYLHVGELTEVVKMFFLKVFYNRSNNQGKKSFLDSVQAKFEHVKKRVKVMQQEEPQEGVETIQLKSLDDSTELQVNLPNFDSKDPEEIKKQNAFKKLPLKMQNAIKTQSLDAINEVFAEMPVEEGEEILEIFNEGSIIGINALLEDEGEFQQLQEKYQSDAGVNKLEAEGQAESHDTEPTRSVNDPQTEISTADVVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DN21</accession>
    <name>C5DN21_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Anaphase-promoting complex subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0G13508g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555619.1">
        <property type="nucleotide sequence ID" value="XM_002555573.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DN21"/>
    <dbReference type="FunCoup" id="C5DN21">
        <property type="interactions" value="235"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DN21"/>
    <dbReference type="GeneID" id="8293902"/>
    <dbReference type="KEGG" id="lth:KLTH0G13508g"/>
    <dbReference type="eggNOG" id="KOG4640">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_424559_0_0_1"/>
    <dbReference type="InParanoid" id="C5DN21"/>
    <dbReference type="OMA" id="PACERII"/>
    <dbReference type="OrthoDB" id="2110451at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070979">
        <property type="term" value="P:protein K11-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024789">
        <property type="entry name" value="APC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024790">
        <property type="entry name" value="APC4_long_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13260">
        <property type="entry name" value="ANAPHASE PROMOTING COMPLEX SUBUNIT 4 APC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13260:SF0">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12896">
        <property type="entry name" value="ANAPC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Anaphase-promoting complex subunit 4 long" evidence="6">
        <location>
            <begin position="227"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00016067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR25182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="645" mass="71896" checksum="029E61FE7DFEFCE7" modified="2009-07-28" version="1">MFDKWQFKRLIKLPKHRGVVWNPRLSLYLVLGDKTVSVNRAIDGQRIAAVNLRGDGQLIGAQWELAEGKLFALFFGNGAVRIYDCATGGSLREVVDLVDEGQSADVTVDTAVWSGAQWAQFRQEYKGLNIEITKQLPGMVRLVRDAKQLSVVPLETGKLRWLADPGKDAAIFLGHDEKSGDYLMTVDGLLSVRVPSGIKKMHKILRQGEDGTFVGVGADGTVQWVVLRFLNSALMRELIRYSGQIRFLCSYLTENINMCKTDLIEPYRQFLTRIADAYEGGELASRFRDIILSGYIPEDLEDWLCNSIGDKNYKRWKQLSTRMYGDLNNVLALAVIPACERLILAAEQLQGIHKGLKLQEFGLASPQEFESAEVTELITGCQALLHASLELVVDLNRDTRLHAIFADWFYDVVMETVDEDYKRPQNRDSHQDCYGDRALALDQYISHCWENSEVWTRFERLLPQLAADADISATKLDHIYTRAWVLEQVHVTSPVRACDVDSVEVLDVQACNKVIMVCRVQTKEGSGATVVTVEPPIADAEPVLQSMKLGMDHVIKAALVLPEGAADNTAVVVALQHSGQQNASVRASCVQFGAHVMHSEHSWEELAPKNEAHEPVAFLPVHVSATRDGVAVHSDTDVAVFENIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>C5DN88</accession>
    <name>C5DN88_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0G15026p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0G15026g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CCNDBP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555686.1">
        <property type="nucleotide sequence ID" value="XM_002555640.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DN88"/>
    <dbReference type="FunCoup" id="C5DN88">
        <property type="interactions" value="89"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DN88"/>
    <dbReference type="GeneID" id="8293971"/>
    <dbReference type="KEGG" id="lth:KLTH0G15026g"/>
    <dbReference type="eggNOG" id="ENOG502QZAU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067849_0_0_1"/>
    <dbReference type="InParanoid" id="C5DN88"/>
    <dbReference type="OMA" id="WIDTWEI"/>
    <dbReference type="OrthoDB" id="4088536at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1410.10">
        <property type="entry name" value="I/LWEQ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026907">
        <property type="entry name" value="GCIP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049317">
        <property type="entry name" value="GCIP-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049318">
        <property type="entry name" value="GCIP_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15492">
        <property type="entry name" value="CYCLIN D1-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15492:SF1">
        <property type="entry name" value="CYCLIN-D1-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20936">
        <property type="entry name" value="GCIP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13324">
        <property type="entry name" value="GCIP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-D1-binding protein 1-like N-terminal" evidence="8">
        <location>
            <begin position="48"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-D1-binding protein 1-like C-terminal" evidence="9">
        <location>
            <begin position="220"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="210"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF20936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR25249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="355" mass="39422" checksum="66B5D788B48A1D15" modified="2009-07-28" version="1">MTEASTQQDIRALVATARADFVESFASREQLRALKPTTSLKDSDASTELDKLSRIIKAHATKVGIIYHPGKFHKNVTPAFKELQLFSNSIFFLLSLLPLLHTDKIADYFTDELDSAVLGLLSGVKGLCDEIDSLLDGKAAPAPGNDVPETAEHDLRLVSVGKIWASCDSLQELAKLGNLGLLNKRIGASVKLITDSLSELDEWLQDPELESQDPFGLESEPESDAERDTSGDKEVLAEMVKFLEAWQQKLKMIRLLLSSFSKSIASEEYKSKEPLALQLAKLNTLHAVVVEKVDELISTVFMTGQDFDPEDEEITELVSLLNDSLRKMVQIIKALNKGDEKKGKWIEVWDAKYFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNG0</accession>
    <name>C5DNG0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0G16654p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0G16654g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555758.1">
        <property type="nucleotide sequence ID" value="XM_002555712.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNG0"/>
    <dbReference type="FunCoup" id="C5DNG0">
        <property type="interactions" value="144"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNG0"/>
    <dbReference type="GeneID" id="8294045"/>
    <dbReference type="KEGG" id="lth:KLTH0G16654g"/>
    <dbReference type="eggNOG" id="ENOG502RZB6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046031_0_0_1"/>
    <dbReference type="InParanoid" id="C5DNG0"/>
    <dbReference type="OMA" id="AEMMMQQ"/>
    <dbReference type="OrthoDB" id="4069381at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021017">
        <property type="entry name" value="Mediator_Med2_fun"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11214">
        <property type="entry name" value="Med2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR25321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="294" mass="31902" checksum="055A9FB37CA3FE3B" modified="2009-07-28" version="1">MPFSISEDIYIDTSSRKVSKKRSCRALLRTMASESVHQSRLTQCFDEIMKTAAEMLVQQQIKSIQLSSDIAPGYTQKQYKSLGDKVHAFHSVLDDLDLTLSASKSCVDKLALEAQERKQEAEKQRIKEEEEARKKLSEVQKPETSNTATSFPESTPGTMLNEISKTGIAGADSGSRGSNNNVATGAAYANDFNELNDLDLSMFGGMEQNDLGLADFGENAMNSNANGGKDNTHEGGFNAAITPGNNEGTNGNQGTDVSSANPESYLTLNDFNDLGLDWNNTEGQGGLDMNEFNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2023-06-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNH0</accession>
    <name>C5DNH0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0G16962p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0G16962g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555768.1">
        <property type="nucleotide sequence ID" value="XM_002555722.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNH0"/>
    <dbReference type="GeneID" id="8294059"/>
    <dbReference type="KEGG" id="lth:KLTH0G16962g"/>
    <dbReference type="HOGENOM" id="CLU_1475429_0_0_1"/>
    <dbReference type="InParanoid" id="C5DNH0"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR25331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="183" mass="20348" checksum="E48AEA96C9F7057C" modified="2009-07-28" version="1">MMAPSSSFINREQLYGYSSNVRGRVLKQHELHGDGKTGPGCLAAHTHVSQNSRQALLIEAILRSRKSQKRHSLAPAQALLRSKCPNFSILATQITTRANEHQSTHHSSLRLGSVPFKSHSRRTPAHIYGPSFLNECSRHMLHRLSNLNMLLSTGSAASRNKNCTESDVATREVAQNCTRQRIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>C5E1Y1</accession>
    <name>C5E1Y1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Ubiquitin-like modifier-activating enzyme ATG7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Autophagy-related protein 7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0H00572g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 and ATG8 for its conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Autophagy is essential for maintenance of amino acid levels and protein synthesis under nitrogen starvation. Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Preautophagosomal structure</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the ATG7 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555904.1">
        <property type="nucleotide sequence ID" value="XM_002555858.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E1Y1"/>
    <dbReference type="FunCoup" id="C5E1Y1">
        <property type="interactions" value="793"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E1Y1"/>
    <dbReference type="GeneID" id="8294216"/>
    <dbReference type="KEGG" id="lth:KLTH0H00572g"/>
    <dbReference type="eggNOG" id="KOG2337">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012998_2_1_1"/>
    <dbReference type="InParanoid" id="C5E1Y1"/>
    <dbReference type="OMA" id="RQIWDAI"/>
    <dbReference type="OrthoDB" id="338614at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019778">
        <property type="term" value="F:Atg12 activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019779">
        <property type="term" value="F:Atg8 activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032446">
        <property type="term" value="P:protein modification by small protein conjugation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000243">
        <property type="entry name" value="Ubiquitin-like modifier-activating enzyme ATG7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.100">
        <property type="entry name" value="Ubiquitin-like modifier-activating enzyme ATG7 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.70">
        <property type="entry name" value="Ubiquitin-like modifier-activating enzyme ATG7 N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006285">
        <property type="entry name" value="Atg7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032197">
        <property type="entry name" value="Atg7_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042522">
        <property type="entry name" value="Atg7_N_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042523">
        <property type="entry name" value="Atg7_N_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01381">
        <property type="entry name" value="E1_like_apg7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF3">
        <property type="entry name" value="UBIQUITIN-LIKE MODIFIER-ACTIVATING ENZYME ATG7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16420">
        <property type="entry name" value="ATG7_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0072">Autophagy</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-like modifier-activating enzyme Atg7 N-terminal" evidence="9">
        <location>
            <begin position="10"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="THIF-type NAD/FAD binding fold" evidence="8">
        <location>
            <begin position="304"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR606285-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR30042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="631" mass="70907" checksum="9A3EF2F2123CF242" modified="2009-07-28" version="1">MSDPHLKFVAPCQSFLDTSFFQELARLKLDVLKLDSAIKELTSSIGISHIPRGSPCAHLFLDSHSFGSRERKEGEMLIKGSLYNFNTIEEFKKVDKTRFLRDRADELWKEGIEDPNDCVKFSIISFADLKAFKFFYWVCVPCFQLDSLTASVRGSGPFKSTSKFDEWFAAHPGCWSCLVGESGELMEFNKVNSVRSSTLCMRDTSTVDMVPSALAKSFISLLKHFRSDLKEVRLIFIRSETASSFWVDTDMSSILAPDNTKLKVTGWERNLQNKLTPRAVDLSSLIDPLHVADQSLDLNLKLMKWRIAPEINLDIIKNTSVLLLGSGTLGCYVARVLLAWGVRKITFVDNGTVSFSNPVRQPLFTFNSCGKPKAAAAADSLREIFPLVEAEGVELAVPMIGHPITSESKQNEDYKRLLELIKAHDVVFLLMDSRETRWLPTVMGYAEDKIVINAALGFDSYLVMRHGNYETDQKADRLGCYFCQDVVAPSDSLTDRTLDQMCTVTRPGVALMAASQSVELLVSLLQHPMRNGGRPDEKVILGDLPHQIRGFLSEFKTLQLRVTAYEHCSACSSIIVEEFKSRGWDFVRDALNDYKTVEDLCGLSEVQLKAEEVMNDVLDEWDDDGEDGELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>C5E203</accession>
    <name>C5E203_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0H01122p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0H01122g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555926.1">
        <property type="nucleotide sequence ID" value="XM_002555880.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E203"/>
    <dbReference type="FunCoup" id="C5E203">
        <property type="interactions" value="1608"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E203"/>
    <dbReference type="GeneID" id="8294239"/>
    <dbReference type="KEGG" id="lth:KLTH0H01122g"/>
    <dbReference type="eggNOG" id="KOG0270">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023867_0_1_1"/>
    <dbReference type="InParanoid" id="C5E203"/>
    <dbReference type="OMA" id="CFVPRGV"/>
    <dbReference type="OrthoDB" id="270624at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044285">
        <property type="entry name" value="PWP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14091">
        <property type="entry name" value="PERIODIC TRYPTOPHAN PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14091:SF0">
        <property type="entry name" value="PERIODIC TRYPTOPHAN PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="289"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="33"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="265"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="542"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="273"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="568"/>
            <end position="579"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR30064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="579" mass="63675" checksum="76230FA4DE019D9A" modified="2009-07-28" version="1">MISSTTWVPRGFPSEFPEKYELDDEEMERINQLAQLNLDDAKDGIESEHDDSELGGEEKNSNEASVRGSAKLASQADNDDNLEEYDMEHYGVKEDEMLGEGAEASMFPGLSDEVKFHEGEDGEDAILSLPTQQDSQEEKQELQVYPTDNMVLATRTEDDVSYLDVYVYDDGAGFHDAEVNQEPGDDKDPDVARGLVRDSSLYVHHDLMLPAFPLCVEWVNYKPGSSSDDAANFAAVGSFDPQIEIWNLDCVEKAFPDMILGDPHATSNVGGSKSKKNKKNKKNKNKHVLTHHTDAVLSLAHSKQFRAVLASTSADHTVKLWDLNEGIAARSVASIHSNKNVSSSQWHSTSGSVLLTGGYDSRVALSDVRIAEDSQMSKYWSVMTGEDVECVQFADENTFMCGTDSGNVYSFDIRQGEGSKPLWTLNAHDAGISALNVNPFIPNLLATGAMGEKTVKFWKISESGPSMVLSRDFGVGNVLTTSFAPDIEVAGNIVIGGVDKGLKLWDVFTNRSIRKNLSKELSSLQRQARAEASKIGRASRIARKYTKNDNPDTVVTVDDQGEDEEEGDGQRESGWEDTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2D5</accession>
    <name>C5E2D5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0H04048p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0H04048g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556058.1">
        <property type="nucleotide sequence ID" value="XM_002556012.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2D5"/>
    <dbReference type="GeneID" id="8294371"/>
    <dbReference type="KEGG" id="lth:KLTH0H04048g"/>
    <dbReference type="eggNOG" id="KOG0568">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019422_1_0_1"/>
    <dbReference type="InParanoid" id="C5E2D5"/>
    <dbReference type="OMA" id="HNANSAF"/>
    <dbReference type="OrthoDB" id="1922282at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018961">
        <property type="entry name" value="DnaJ_homolog_subfam-C_membr-28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39394:SF1">
        <property type="entry name" value="DNAJ HOMOLOGUE SUBFAMILY C MEMBER 28 CONSERVED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39394">
        <property type="entry name" value="YALI0E31793P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09350">
        <property type="entry name" value="DJC28_CD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DnaJ homologue subfamily C member 28 conserved" evidence="1">
        <location>
            <begin position="202"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR30196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="408" mass="47444" checksum="CDAA17F9247ACE36" modified="2009-07-28" version="1">MIKRAFNTTVNRFENTQGDWGYLKQRLTQLKEEAAPDDNDALCGLIKGVVKENPSIEKLYDELPRKEYEDRFRSQIQASKLKRFVNKESRETALSRPWTGEEHYTDTSLRMIMDGVKNEGGSSGSSRNLGFSSTFQATKESNRYARRAVKTRLRLETAQDRIIDYKIDKDEHKSDDIEASEFRVLYAEKFTPIGSFEKLKSIADVRIEESMKKGEFDSVKKIRGQKLSSTQPLPYVDRTEHHLNDILVRQNISPPWIEKQGSVNKDIMVFRDDLLRKFQSELIAALNLRGFFRTEVPSNSELNQHGKLNSVKESVFSDWKSTLKIGSRIKQLNDSLRSYNLQAPLPAQKFYLQADRELQRVLKETDISRLYRDEFSRQKVLHNNAKAANESQATSNSQFSWPKKWRIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2E6</accession>
    <name>C5E2E6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Heat shock transcription factor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Heat shock factor protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0H04290g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">DNA-binding transcription factor that specifically binds heat shock promoter elements (HSE) and activates transcription.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homotrimer. Homotrimerization increases the affinity of HSF1 to DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the HSF family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556069.1">
        <property type="nucleotide sequence ID" value="XM_002556023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2E6"/>
    <dbReference type="STRING" id="559295.C5E2E6"/>
    <dbReference type="GeneID" id="8294382"/>
    <dbReference type="KEGG" id="lth:KLTH0H04290g"/>
    <dbReference type="eggNOG" id="KOG0627">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017670_0_0_1"/>
    <dbReference type="InParanoid" id="C5E2E6"/>
    <dbReference type="OMA" id="DSILMPY"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032993">
        <property type="term" value="C:protein-DNA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000027">
        <property type="entry name" value="Heat shock transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF427">
        <property type="entry name" value="HEAT SHOCK FACTOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00056">
        <property type="entry name" value="HSFDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00434">
        <property type="entry name" value="HSF_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="13">
        <location>
            <begin position="200"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="23"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="132"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="394"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="488"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="395"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="429"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="452"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="503"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="532"/>
            <end position="543"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00059868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00062447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00068818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00084017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR30207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="569" mass="62681" checksum="7166E29A1E376284" modified="2009-07-28" version="1">MQANNAHHSAGPALNEDEIENIINPSDPIVGGTHGTGLGADDGSHSVVEDIVNPSWDPAMTMSPGQLDHPSQNIAPFSPFMANSPLAAPPDHNNVIRRGTPVGLYQQQGSPSQPPAADSLLSDSILMPYRNRLLKQSRPKTSSPQPPPRRKLTTTKTRPAFVNKLWSMVNDSANQKLIHWSADGKSFVITNREHFVHEILPKYFKHSNFASFVRQLNMYGWHKVQDVRSGSIHGNSDERWQFENENFVRDCEDLLENIVRQKPSTNPSKDVLVGQNGEEMDIGILLSELETVKFNQIAIAEDLKRMSKDNELLWKENMMARERHQAQQQALNKILHLLASLMGSNTQKLLGSDLVNELAQTNANLNDASNVAHGGWDLANMNGLTRPRLLLKNRTPATSQHSSTGDLSSLHIGHSNSPIQEIDRSSIYPSNGSSSAGASTTPGGGRAPDTMLESNSPVDTSFFNDLHSNIKKQGESIQELQDWITKMSPSQEELGSITPEGTVPQQLAQSPAKTQQPNSKFDLQDYLSTSDPSALAPLLPEEPLVQEEKRSSDSDIEEISNPPKKQRTS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-05-31" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2E7</accession>
    <name>MPS2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName>Monopolar spindle protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">MPS2</name>
        <name type="ordered locus">KLTH0H04312g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the spindle pole body (SPB) required for insertion of the nascent SPB into the nuclear envelope and for the proper execution of spindle pole body (SPB) duplication.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Spindle pole body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MPS2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556070.1">
        <property type="nucleotide sequence ID" value="XM_002556024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2E7"/>
    <dbReference type="SMR" id="C5E2E7"/>
    <dbReference type="FunCoup" id="C5E2E7">
        <property type="interactions" value="164"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2E7"/>
    <dbReference type="GeneID" id="8294383"/>
    <dbReference type="KEGG" id="lth:KLTH0H04312g"/>
    <dbReference type="eggNOG" id="ENOG502RYE7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069890_0_0_1"/>
    <dbReference type="InParanoid" id="C5E2E7"/>
    <dbReference type="OMA" id="FIYAKDF"/>
    <dbReference type="OrthoDB" id="4035046at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005816">
        <property type="term" value="C:spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071988">
        <property type="term" value="P:protein localization to spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030474">
        <property type="term" value="P:spindle pole body duplication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031433">
        <property type="entry name" value="Mps2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17060">
        <property type="entry name" value="MPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0175">Coiled coil</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0206">Cytoskeleton</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0539">Nucleus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000409158" description="Monopolar spindle protein 2">
        <location>
            <begin position="1"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="296"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="117"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="386" mass="44080" checksum="3FF919CDD591D378" modified="2009-07-28" version="1">MDTERHATLLLDLVWPEVDEKAQGFIYAKDFPLVVSRMEEILNRGKLERDRAQLVSETGREILRKFGSDQEFFKVYKEDFRELFDGLVGTSFKSAVKSCAGDGVLDRLQDSQAVDGIQDEKTSSHALQEEVMRLREQVRVLSSKNDEKDREITARDEIIADLQGKDASPAGSPRSLQRMRTLQARVTSLEDELSFRDEVIREKDRELLNLTKRVGEFKDKYQFLEREFQFYKGHREQKSPDSIKEATRHEFIISELRRKITEQSEIIGQMRMQVEAKPGALHPQGIGSTAGLPLNLPLRLVLRLIIGAILAYLAFDIGIRSLKAVGGLFGSSSPATLTPKSELSWWEQNTLLSKLLWFFKDLFDTYNLDAGRDEVVSANYDKLFGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2Q3</accession>
    <name>C5E2Q3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">precorrin-2 dehydrogenase</fullName>
            <ecNumber evidence="2">1.3.1.76</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0H06820g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; siroheme biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.76" evidence="2"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556176.1">
        <property type="nucleotide sequence ID" value="XM_002556130.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2Q3"/>
    <dbReference type="FunCoup" id="C5E2Q3">
        <property type="interactions" value="133"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2Q3"/>
    <dbReference type="GeneID" id="8294492"/>
    <dbReference type="KEGG" id="lth:KLTH0H06820g"/>
    <dbReference type="eggNOG" id="ENOG502RYIW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011276_8_5_1"/>
    <dbReference type="InParanoid" id="C5E2Q3"/>
    <dbReference type="OMA" id="PTGCKLT"/>
    <dbReference type="OrthoDB" id="1721126at2759"/>
    <dbReference type="UniPathway" id="UPA00262">
        <property type="reaction ID" value="UER00222"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004325">
        <property type="term" value="F:ferrochelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043115">
        <property type="term" value="F:precorrin-2 dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019354">
        <property type="term" value="P:siroheme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.110">
        <property type="entry name" value="Siroheme synthase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3280.10">
        <property type="entry name" value="Siroheme synthase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028161">
        <property type="entry name" value="Met8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028162">
        <property type="entry name" value="Met8_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028281">
        <property type="entry name" value="Sirohaem_synthase_central"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35330">
        <property type="entry name" value="SIROHEME BIOSYNTHESIS PROTEIN MET8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35330:SF1">
        <property type="entry name" value="SIROHEME BIOSYNTHESIS PROTEIN MET8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13241">
        <property type="entry name" value="NAD_binding_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14823">
        <property type="entry name" value="Sirohm_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14824">
        <property type="entry name" value="Sirohm_synth_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75615">
        <property type="entry name" value="Siroheme synthase middle domains-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Siroheme synthase central" evidence="7">
        <location>
            <begin position="179"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="Siroheme biosynthesis protein Met8 C-terminal" evidence="6">
        <location>
            <begin position="210"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF14823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR30314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="296" mass="33389" checksum="3C49DFC3E110F996" modified="2009-07-28" version="1">MLSLQLAHQLSGKHILLVGAGEVAMTRIPKLLPTGCKLTVIAPEIHGDMWKHFDVPEDNDTVEHTDEHWSPEKNKIFQIFRRGFRNTDIVLHGNGLTDNADAQSFLNGELDPIESYDHNKASGWHMIMTCIPDPVLSEKIYRGAKLVLGQHVLCNVADNPPLCDFYFGSNVTLGKGGKPIQIMLSSNGNSPRFTALLKNEIERQFGDLPIGESVAKLGQLRARIRKISEERCPASLSKPELIKYRMEWIRNCTDAFGVSHCHEINVDKTVTLFEDMFASMSLEQPDNEKYMKEYLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2U4</accession>
    <name>C5E2U4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0H07766p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0H07766g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the alkylbase DNA glycosidase AlkA family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556217.1">
        <property type="nucleotide sequence ID" value="XM_002556171.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2U4"/>
    <dbReference type="FunCoup" id="C5E2U4">
        <property type="interactions" value="87"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2U4"/>
    <dbReference type="GeneID" id="8294535"/>
    <dbReference type="KEGG" id="lth:KLTH0H07766g"/>
    <dbReference type="eggNOG" id="KOG1918">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_72_4_1"/>
    <dbReference type="InParanoid" id="C5E2U4"/>
    <dbReference type="OMA" id="FMFILWR"/>
    <dbReference type="OrthoDB" id="415889at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032993">
        <property type="term" value="C:protein-DNA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032131">
        <property type="term" value="F:alkylated DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008725">
        <property type="term" value="F:DNA-3-methyladenine glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043916">
        <property type="term" value="F:DNA-7-methylguanine glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006285">
        <property type="term" value="P:base-excision repair, AP site formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006307">
        <property type="term" value="P:DNA alkylation repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.30:FF:000004">
        <property type="entry name" value="DNA-3-methyladenine glycosylase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051912">
        <property type="entry name" value="Alkylbase_DNA_Glycosylase/TA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000035">
        <property type="entry name" value="Alkylbase_DNA_glycsylse_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43003">
        <property type="entry name" value="DNA-3-METHYLADENINE GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43003:SF5">
        <property type="entry name" value="DNA-3-METHYLADENINE GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00516">
        <property type="entry name" value="ALKYLBASE_DNA_GLYCOS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HhH-GPD" evidence="4">
        <location>
            <begin position="71"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR30355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="274" mass="31521" checksum="C5E42626FEC30C37" modified="2009-07-28" version="1">MKRSREETVELPQDFAEKHIDTFVKGCHHILGVDPSLYDALLSKDFQLFLKREQPPMDLNHHFIKLASSILAQQISGAAANSVKNKLMAYYGGHFPTYQEVVETFHKKDVQELRDCGLSARKVQYLESLANYFNDNEKAIEALFEQDNEEIASQLVANIKGIGPWSAKMFLVTSLERMDVFAADDLGIARGCSKYLDCRPEILKELMSKRVQGKTKRSKIVHKKKNWKIYDEDIVEKCGEMFAPYRSIFMFILWRLSSTNIDALAKREKDFVAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3A2</accession>
    <name>C5E3A2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0H11638p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0H11638g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. ADCK protein kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556375.1">
        <property type="nucleotide sequence ID" value="XM_002556329.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3A2"/>
    <dbReference type="FunCoup" id="C5E3A2">
        <property type="interactions" value="498"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3A2"/>
    <dbReference type="GeneID" id="8294709"/>
    <dbReference type="KEGG" id="lth:KLTH0H11638g"/>
    <dbReference type="eggNOG" id="KOG1235">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006533_2_5_1"/>
    <dbReference type="InParanoid" id="C5E3A2"/>
    <dbReference type="OMA" id="RCNPEDI"/>
    <dbReference type="OrthoDB" id="427480at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13969">
        <property type="entry name" value="ADCK1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004147">
        <property type="entry name" value="ABC1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045307">
        <property type="entry name" value="ADCK1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051130">
        <property type="entry name" value="Mito_struct-func_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43173:SF19">
        <property type="entry name" value="AARF DOMAIN-CONTAINING PROTEIN KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43173">
        <property type="entry name" value="ABC1 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03109">
        <property type="entry name" value="ABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC1 atypical kinase-like" evidence="2">
        <location>
            <begin position="156"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR30513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="565" mass="64674" checksum="C39E9ACF488E14A3" modified="2009-07-28" version="1">MVQPLLNLARIAPLKLGARGPFRSYSSSAKSFVRPKSPVITTKRVLVVTAFAAAAYVYTDDTAKSAVRHLGVTSKRVGVVAQATMRCVYNYQRTLSANYSSEQERSDALKKCHQLCAKVTLRALETNGGVFIKLGQHIAAMTYLLPPEWTETMVPLQDKCPQSTHQEIEDLFKQDLKIDIQDMFSDFDPEPIGVASLAQVHVATLRSTGEKVAVKCQHPSLKEFVPLDVLLTQTVFNVVDVLFPEYPLTWLADELQNSIYVELDFTKEAENARRTADYFSNYISETALRIPKVIVANKRILIMEYIGGHRLDDLAYLDENHISRAQVSSCLSHIFNNMIFTPNVGIHCDPHGGNLAIRHLEKSRGHHNFEIILYDHGLYRHPTTEMRRDYAKFWLALLDNDVANMKKYAMKFANINEDQFPLFAAAITGRSIDAAQSSDILKPRSDEEIRVMADALMEGSLLGDLMTFLCKVPRVVLLILKTNDLTRHLDECLQSPLSVERTFLIMTQYCARTVYEEVRQNINEQYQKWSLTWIFKEITAWWEYERRKNQLVLYDLGFWFKNRFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3H9</accession>
    <name>C5E3H9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0H13706p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0H13706g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556452.1">
        <property type="nucleotide sequence ID" value="XM_002556406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3H9"/>
    <dbReference type="FunCoup" id="C5E3H9">
        <property type="interactions" value="241"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3H9"/>
    <dbReference type="GeneID" id="8294813"/>
    <dbReference type="KEGG" id="lth:KLTH0H13706g"/>
    <dbReference type="eggNOG" id="KOG4829">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086740_0_0_1"/>
    <dbReference type="InParanoid" id="C5E3H9"/>
    <dbReference type="OMA" id="ISKWNTQ"/>
    <dbReference type="OrthoDB" id="10261563at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019327">
        <property type="entry name" value="WKF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22306">
        <property type="entry name" value="CHROMOSOME 7 OPEN READING FRAME 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22306:SF2">
        <property type="entry name" value="CHROMOSOME 7 OPEN READING FRAME 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10180">
        <property type="entry name" value="WKF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WKF" evidence="2">
        <location>
            <begin position="89"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="200"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="221"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR30590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="259" mass="29030" checksum="68D7D75BD9F54FD5" modified="2009-07-28" version="1">MDHIPAWKRIAIKSQSPEQTPDETALNVTTHLATANLSKREKRRIIRGEPEGGKAKKAANPNKKQKKDKLPREERNLHKEEVLKDQLRYLIDFYREKVGKLPATVYEHKPVRAQLGDPQVEDAEEASADAGVVSVWKFSKQKQNWLLKHILDDVQIPACYDDLLYSYFKDLKGGARAGLEQACRELLDRNEKAVAAEAARVEAAADQEKETAEEDQDAKPPAEATPATAETMVPPSAHQVERAKSLIAHLEQAAKDEQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBG1</accession>
    <name>C5DBG1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">KLTH0A02332p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0A02332g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the patched family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551560.1">
        <property type="nucleotide sequence ID" value="XM_002551514.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBG1"/>
    <dbReference type="FunCoup" id="C5DBG1">
        <property type="interactions" value="549"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBG1"/>
    <dbReference type="GeneID" id="8290357"/>
    <dbReference type="KEGG" id="lth:KLTH0A02332g"/>
    <dbReference type="eggNOG" id="KOG1933">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002359_0_1_1"/>
    <dbReference type="InParanoid" id="C5DBG1"/>
    <dbReference type="OMA" id="WWFDVES"/>
    <dbReference type="OrthoDB" id="6510177at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015918">
        <property type="term" value="P:sterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Putative Patched sphingolipid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1640.10">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053958">
        <property type="entry name" value="HMGCR/SNAP/NPC1-like_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053956">
        <property type="entry name" value="NPC1_MLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032190">
        <property type="entry name" value="NPC1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000731">
        <property type="entry name" value="SSD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45727">
        <property type="entry name" value="NPC INTRACELLULAR CHOLESTEROL TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45727:SF2">
        <property type="entry name" value="NPC INTRACELLULAR CHOLESTEROL TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22314">
        <property type="entry name" value="NPC1_MLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16414">
        <property type="entry name" value="NPC1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12349">
        <property type="entry name" value="Sterol-sensing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82866">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50156">
        <property type="entry name" value="SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0732">Signal</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002948649" evidence="12">
        <location>
            <begin position="20"/>
            <end position="1179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="269"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="349"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="567"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="594"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="624"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="675"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="701"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="761"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="802"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1009"/>
            <end position="1029"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1036"/>
            <end position="1058"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1064"/>
            <end position="1086"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1107"/>
            <end position="1130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1142"/>
            <end position="1165"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="13">
        <location>
            <begin position="566"/>
            <end position="726"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR21118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1179" mass="132455" checksum="42A25CCA37C736C8" modified="2009-07-28" version="1">MKVARCLLVPVIVSNAVLAGDSFFGISFANSKPASCSIYGSCGKKSLFGGELPCPVTEDFVPDPLSEAERESLVDLCGEEWKDVNQVCCNGAQIENLRKNLKKAENLIASCPACDKNFKSLFCHFTCSPQQRVFTNVTETQSSTDGREIVASMNVFLDSHWASEFYDSCKNVKFGATNGYAMDLIGGGAKNYEQFLKFLGDEKPLLGGSPFQINYIYDSQNSGIELFNETVYSCDDETYKCACTDCDKSCPKLKPLRRNHCKVAGLPCFSFVILMVYVSLFVLILGWHIYYFKNRKEPIIFEQDIEDSYAVANNDRIFQEHAYKAYSFDEKVASVLGQISGFSYRHPKLVILATGVVVVLLTVLAWIFGDLETDPINLWVSKSSPKYKEKEFFDENFGPFYRTEQIFIVNETGPVLSYPNLEWWFGVESYITEVLRSEEYQTYQDLCFRPTEDSTCVVESVTQYFNQDLPKEGDWENRLKSCTESPVNCLPSFQQPLKPNLLFSDNDVFKSNALVVTLLLSNHSNSALLWEKQLEDYLLNLDLPHGLRLSFNTEMSLEKELNRNNDIYIVLVSYLLMFAYASWALRKKGGGSRLLLGFSGILIVLSSVSSATGVLSILGLKSTLIIAEVIPFLILAIGIDNIYLITHEFDRISEGEQTLEVEHIMKKALQKISPSILLSLLCQLCCFLIATFVSMPAVRNFAIYSAVSLVFNVLLQLSAYVSILTLYERTFSATENTVSSESTPSKLKSAYVELLTKKRKIVGIFVSLTLFSLFFIPYIEIGLDQTLAVPQDSYLVDYFRDIYKYLNVGPPVFFVVKNLDLTSRSNQKKVCGKFTTCDDLSLANTLEQERKRSTVVEPVTNWFDDFMMFLNPQLDTCCRLKKGTEDVCPPSFPSRRCETCFSESEWFYDMSGFPTGDEFMHYFNIWINSPSDPCPLGGKAPYSSAITYNKTSIKSSTFRSAHKPLKSQQDFIEAYKDAERISKSLFELDVFAYSPFYIFFVQYGSLVSLSLKLLASSVLLVLLVSWMFLGSLKTAALVGLTVAMILTDIGALMFFLGISLNAVSLVNLIICVGIAVEFCVHIARAFTIVPSNIKTDRDSRMVHAIETVGGSVFQGITLTKFIGVSILAFTHSKIFQVFYFRMWFILIIASCAHALFFLPAALSLVGGKSYADSEVELED</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBJ5</accession>
    <name>C5DBJ5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0A03146p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0A03146g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="CAR21152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_002551548.1"/>
    </dbReference>
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        <property type="interactions" value="40"/>
    </dbReference>
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    <dbReference type="KEGG" id="lth:KLTH0A03146g"/>
    <dbReference type="eggNOG" id="ENOG502QQG0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022832_0_0_1"/>
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        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
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    </dbReference>
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        <property type="entry name" value="KPR-Related"/>
    </dbReference>
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        <property type="entry name" value="OUTER SPORE WALL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21708">
        <property type="entry name" value="PROBABLE 2-DEHYDROPANTOATE 2-REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    </feature>
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        <location>
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    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21152.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="635" mass="72098" checksum="245775F4F16B506A" modified="2009-07-28" version="1">MIILSCLSFPSLNRYCDLLSRYSGKHTIVLVDASFAAQLEKLVLDKFYDTCACVLSVLCDVETRQLSSGSYALVNDSCKFYLGLTYARKGFNKQANKQDRLSVNLQCVQNTFFDESSTLNAMVAQLEGTNVDTIIKLAPEESCEMALKVWEHIIPRISLHILSIVFEQFDYDKLLENSSSRFIFEDLVRELSSICYAQCGEVSEKYLKSKSGPRNPPTSAEDWISQLDFDKVLEETKRRKRQMDRSTINEYPEFLTLSFEAYCFYHRLEFPAHILLHQPIQLASAYRINYSSINFLYGFYSRLLAISGFSIEGEPNKVSAPSLLFGIRTQLANQSEQTLVSKHCLNDDKNAPGSASKSKGVKSKLRARFKKIGHGNRERTSGATYSSEDPELSDEESGSSLDNLLAGDARDRSSTDPEDTGEAQAGRDFMFWCSEEFQDANSEPEIDDNDNSSIVDELIKLPNFRKRNFRKQGTQAELTLTTVPLFEQEIRSDPFAMSRYYFDAYKLGDPKTFLSLKERDSRPASARRFQRRYPRERAGVTGASTLPENYFRDHVDLMKRVNMGGILSVTTSRYGHVDSSQKMLDNWNRGLGYLKTLALRESASQVGASCHVVREPPARLQLRHSRRSSFSPEVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBS5</accession>
    <name>C5DBS5_LACTC</name>
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        <submittedName>
            <fullName evidence="2">KLTH0A04994p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0A04994g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002551628.1"/>
    </dbReference>
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    <dbReference type="FunCoup" id="C5DBS5">
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    </dbReference>
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    <dbReference type="KEGG" id="lth:KLTH0A04994g"/>
    <dbReference type="eggNOG" id="ENOG502S3TQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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    <dbReference type="InParanoid" id="C5DBS5"/>
    <dbReference type="OrthoDB" id="3981113at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
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            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="141"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="45"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="195" mass="21776" checksum="A74EF10B561E7763" modified="2009-07-28" version="1">MEDSSIFSQSSKDVSSAISLYNMPTGEDASIRGLLDYDPSEEAQSSGISRPLSRSSVTSSLSMVATKDGVEGRRVHRYGIPQYSLNLLDAMSQPPPLKGKVHTRQNAQSHHLSPQENKEDSETQLYQDLMHSPPITLKEKMQLFRDNSESSHLDYSEESYQEGSTSLSGKDQLHSLHSYKKDIASERDDSDDYSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBT5</accession>
    <name>C5DBT5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0A05214p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0A05214g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
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                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002551638.1"/>
    </dbReference>
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    <dbReference type="eggNOG" id="KOG4400">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049076_1_0_1"/>
    <dbReference type="InParanoid" id="C5DBT5"/>
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        <property type="component" value="Chromosome A"/>
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    <dbReference type="GO" id="GO:0031499">
        <property type="term" value="C:TRAMP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:RNA binding"/>
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    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071031">
        <property type="term" value="P:nuclear mRNA surveillance of mRNA 3'-end processing"/>
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    <dbReference type="GO" id="GO:0071039">
        <property type="term" value="P:nuclear polyadenylation-dependent CUT catabolic process"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071036">
        <property type="term" value="P:nuclear polyadenylation-dependent snoRNA catabolic process"/>
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    </dbReference>
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        <property type="term" value="P:polyadenylation-dependent RNA catabolic process"/>
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    </dbReference>
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        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Air1/2_Saccharomycetales"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049024">
        <property type="entry name" value="AIR2-like_ZnK4"/>
    </dbReference>
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        <property type="entry name" value="TRAMP_AT-DNA-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46543">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46543:SF1">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21759">
        <property type="entry name" value="AIR2-like_ZnK4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018162">
        <property type="entry name" value="PolyA_pol_Air1/2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="9">
        <location>
            <begin position="93"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="9">
        <location>
            <begin position="131"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="9">
        <location>
            <begin position="192"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="206"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="262"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="266"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="335"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="359"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR21242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="396" mass="44834" checksum="066DF71D69671A31" modified="2009-07-28" version="1">MSLKNPSSSQTHDNNRNAYQENRMASLLSEYESHDTLSFVKSTTPTPGASEGILAPSIDEVDSNPEDLRYLRGKGRYFGVDDPSDPSLDAEPKCNNCSQRGHLKRNCPHVICAYCGIMDDHYTQQCPKAIKCANCGEEGHYRSQCPRQARRIYCTECNSKNHARERCPSIWRSYYLRERTFHRTLHIERVFCYNCGHQGHFGDDCSMRRSSKVPNEDGSAFSGENLPQELKAEYSRNLKESRLRFTQEFTFDRRQRLAAPLGEGLVGKREDTASSRRNSERKKRNKPDFGARPSQGFPKPARNVSEPFKRNPPGRRPQKSFRASSQGNRGGRGSSPGASNSSWGDFEPLRGSRAHNKPSQRSSKFSQRSNKSSQRSAKPSSPRNAFPFKKRKKGPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBV6</accession>
    <name>C5DBV6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Vacuolar protein sorting-associated protein 27</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0A05676g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the ESCRT-0 complex which is the sorting receptor for ubiquitinated cargo proteins at the multivesicular body (MVB) and recruits ESCRT-I to the MVB outer membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the ESCRT-0 complex composed of HSE1 and VPS27.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Endosome membrane</location>
            <topology evidence="1 10">Peripheral membrane protein</topology>
            <orientation evidence="1 10">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the VPS27 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551705.1">
        <property type="nucleotide sequence ID" value="XM_002551659.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBV6"/>
    <dbReference type="FunCoup" id="C5DBV6">
        <property type="interactions" value="138"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBV6"/>
    <dbReference type="GeneID" id="8290509"/>
    <dbReference type="KEGG" id="lth:KLTH0A05676g"/>
    <dbReference type="eggNOG" id="KOG1818">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011862_2_0_1"/>
    <dbReference type="InParanoid" id="C5DBV6"/>
    <dbReference type="OMA" id="HTWGGNT"/>
    <dbReference type="OrthoDB" id="957735at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033565">
        <property type="term" value="C:ESCRT-0 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15760">
        <property type="entry name" value="FYVE_scVPS27p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21385">
        <property type="entry name" value="GAT_Vps27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16979">
        <property type="entry name" value="VHS_Vps27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000039">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017073">
        <property type="entry name" value="HGS/VPS27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002014">
        <property type="entry name" value="VHS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049425">
        <property type="entry name" value="Vps27_GAT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47794">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47794:SF1">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23625">
        <property type="entry name" value="UIM_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00790">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21356">
        <property type="entry name" value="Vps27_GAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036956">
        <property type="entry name" value="Hrs_Vps27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00726">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00288">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50179">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0967">Endosome</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="VHS" evidence="14">
        <location>
            <begin position="20"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="FYVE-type" evidence="13">
        <location>
            <begin position="173"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="236"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="312"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="470"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="521"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="238"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="312"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="470"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="577"/>
            <end position="590"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR036956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR21263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="625" mass="70645" checksum="920ED4920D3D5946" modified="2009-07-28" version="1">MSAAVSTPSQLDSLIQRATSESIPNGEIDLSLALEVSDVIRSRRVPPKECMRCIKKRIMSSRSNPNRSLSAWRLTEVCLKNGGVPFIKEVCSREFMDSLEHAILQEESNDELEILCKGMLQDLYVAFRNDSQLNYVSKVYQRLEAKGVDFPSNPSRHSELAAAMFDSKTPADWIDSDTCMICSNKFSLLNRRHHCRSCGGIFCQEHSSRFIPLPDLGIYDNVRVCDNCFDDYETKKQSDKKRKKKKKGHGKSSSVRDQEEEELKKAIELSLRDARSEDTFVPVVPVQKAPSPQAIDEEEDEDLKAAIAASLRESEEAKRRQQERQQTPVAEGYSVPDVPPPLPSNELTTSEEDDIQLFASLVERMKYQPPTAILEDTQLQQLYRKVMGTVPKLNIALSETSHKYNTLMNMNGKISDIMNIYDSLLEKQLQSINLNQQYFMPQLPSDPYAYYNVGQQNSPHVNPAALPQQNQHIQVSSPALNRQHVSESKVPSMPASELRPLEGHASPNHIEQLKSIAIEQEAPQKQSLSSPRPYPDETQEEQNPCLSDPYPNGISKNGPAQAPSDPPYPNDDEEKEESLRQAHPKLDKITNFDFPTVPVQQPPVEEHVKEEPEEANEQEQLLIEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBW6</accession>
    <name>C5DBW6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Lactoylglutathione lyase</fullName>
            <ecNumber evidence="3 9">4.4.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Glyoxalase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0A05896g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(R)-S-lactoylglutathione = methylglyoxal + glutathione</text>
            <dbReference type="Rhea" id="RHEA:19069"/>
            <dbReference type="ChEBI" id="CHEBI:17158"/>
            <dbReference type="ChEBI" id="CHEBI:57474"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="EC" id="4.4.1.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 1 zinc ion per subunit. In the homodimer, two zinc ions are bound between subunits.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the glyoxalase I family.</text>
    </comment>
    <dbReference type="EC" id="4.4.1.5" evidence="3 9"/>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551715.1">
        <property type="nucleotide sequence ID" value="XM_002551669.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBW6"/>
    <dbReference type="FunCoup" id="C5DBW6">
        <property type="interactions" value="235"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBW6"/>
    <dbReference type="GeneID" id="8290519"/>
    <dbReference type="KEGG" id="lth:KLTH0A05896g"/>
    <dbReference type="eggNOG" id="KOG2944">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046006_0_1_1"/>
    <dbReference type="InParanoid" id="C5DBW6"/>
    <dbReference type="OMA" id="MGDAWGH"/>
    <dbReference type="OrthoDB" id="16820at2759"/>
    <dbReference type="UniPathway" id="UPA00619">
        <property type="reaction ID" value="UER00675"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004462">
        <property type="term" value="F:lactoylglutathione lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07233">
        <property type="entry name" value="GlxI_Zn"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="entry name" value="2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004361">
        <property type="entry name" value="Glyoxalase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018146">
        <property type="entry name" value="Glyoxalase_1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC_core"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00068">
        <property type="entry name" value="glyox_I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10374:SF30">
        <property type="entry name" value="LACTOYLGLUTATHIONE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10374">
        <property type="entry name" value="LACTOYLGLUTATHIONE LYASE GLYOXALASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00934">
        <property type="entry name" value="GLYOXALASE_I_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00935">
        <property type="entry name" value="GLYOXALASE_I_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="VOC" evidence="10">
        <location>
            <begin position="44"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="VOC" evidence="10">
        <location>
            <begin position="204"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="7">
        <location>
            <position position="338"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="338"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR604361-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR604361-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR21273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="346" mass="39750" checksum="048FEB6841CA9E72" modified="2009-07-28" version="1">MHSVARYLGRLTNVYRLPAIRKMSSEAKFYPIKIEAAQNDASLKLNHTCVRVKDPQRSVEFYKSKFGMELVGHKKFPDAKFDLYFLSFPKSNLQHNSRGEIDVFRENGMLELTHNYGTESDPTFKVNNGNEEPYRGFGHICFSVADIEKTCKDLEEKGVAFKKKLTDGRQKNIAFALDPDGYWIELVSYGRSDSGEPVKDEGYKFNHTMVRVKDAQKSLEFYQNVLGMKILDISKHENAKFTLYFLSYDGAKPRWMQESVLELTHNWGTESDPDFQYHNGNSEPQGYGHICISLEDPETLCKEIESQYGDKVQWGVKFNQGKMKNLAFIKDPDGYSIEVVPYGLCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBZ4</accession>
    <name>C5DBZ4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Guanine nucleotide-exchange factor SEC12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">KLTH0A06578g</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Guanine nucleotide-exchange factor (GEF) required for the formation or budding of transport vesicles from the ER.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Endoplasmic reticulum membrane</location>
            <topology evidence="15">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Golgi apparatus membrane</location>
            <topology evidence="15">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14 15">Belongs to the WD repeat SEC12 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551743.1">
        <property type="nucleotide sequence ID" value="XM_002551697.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBZ4"/>
    <dbReference type="FunCoup" id="C5DBZ4">
        <property type="interactions" value="241"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBZ4"/>
    <dbReference type="GeneID" id="8290550"/>
    <dbReference type="KEGG" id="lth:KLTH0A06578g"/>
    <dbReference type="eggNOG" id="KOG0771">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_287583_0_0_1"/>
    <dbReference type="InParanoid" id="C5DBZ4"/>
    <dbReference type="OMA" id="PTVIRIM"/>
    <dbReference type="OrthoDB" id="2013972at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003400">
        <property type="term" value="P:regulation of COPII vesicle coating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000597">
        <property type="entry name" value="Guanine nucleotide-exchange factor SEC12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024977">
        <property type="entry name" value="Apc4-like_WD40_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045260">
        <property type="entry name" value="Sec12-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23284">
        <property type="entry name" value="PROLACTIN REGULATORY ELEMENT BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23284:SF0">
        <property type="entry name" value="PROLACTIN REGULATORY ELEMENT-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12894">
        <property type="entry name" value="ANAPC4_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 15" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="2" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 15" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 15" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 15" id="KW-0813">Transport</keyword>
    <keyword evidence="3 15" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Anaphase-promoting complex subunit 4-like WD40" evidence="17">
        <location>
            <begin position="277"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="440"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="509"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="892"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="442"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="476"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="528"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="540"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="565"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="597"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="642"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="662"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="698"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="740"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="767"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="816"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="847"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="868"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="892"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="936"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="947"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="965"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1036"/>
            <end position="1061"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00061254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU369019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF12894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAR21301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1071" mass="114369" checksum="FAD57C2CD4728D01" modified="2009-07-28" version="1">MSHKRTMKFQQAVYNIGYPAYGARFLNDNVLLVAGGGGEGNNGIPNKLTALQVSFDKRKVIKRFRELTLNENDDSPTTLDAANNIILMGCNENSEKIKATGENLHLRKYVFENEHLKFVASIDMDHSSKFEDYTKLTYMSQDGSVAAIASSKVPTVIRIMNPNLLTETYEIETGNDVKDLHFSPDGKVLSYITSSTLEVISIVTGRFIVRKTDFDKSWALSKIKFIGEDTVLIAAALKKGTGIVLSKVSLKSGTTSVLKTRLVTTKLRGVTSMDVDPTGQLAALSGNDNSIVLVRLKNLTVAKFFKQVHSFAITKVAFSPNGKILASVSAANTIHVILVPDKLATSVPILERIVKLFINFVLIVVVAAAVQISYKYDLHTKAYRYGVAKWNARRDSFIMNDVFRQTTLVGDVVSIETHTNFAPTESSSVNTPIYETTVPELSTTSPYSSSWSSPVPEDPLLTSDASTVEEPEYFPSSLEEISAENESANSEASSSMTVSASMTASISGVQESAVSASEEKPTQATQKSSSESEALLSDSNDISTENGLNKSKAQQQFYEVDKTLEQQPTPTSVQSSDVHSGISLTAGNSKGEEASPSHKISSASSGASSNISLGEPVSKLPSASKASPMDTPIEESSPETRVAVSEESSKSASTVPELTPKIARDVSNEKPVEKSANAIPEASTQALSPALAEAPVKTHSSFSNQEATSLTTTPAFSATGRVASADSPKKANSEFGELQDTERERDFDLKASAGASDAISSEEPVTENKTYDASSPSSFISDTTNTQIASSPIANSVSSTIANSSLSSESKKAKEINTSSPSRSQEPARSEIITTTASTAVEDKSAAPTTLSQAISQDESDGSKAKTESNVIPTQASATGIVSPVQLMASTESSNLNKVPKSSNSAQSETEVAEAKVHLSTPEELQSTEKTDEIKTSSPHISSWGKNPSSNTNAPTNSIALPDDFENRFTPEDETYSEEKRSPIEDEELEADAQIYSEQVYPSQIEVADPLPQSTPEPSVSREPKDTESYSEPVGSQSGDSTAGSSSVSASGSVEETSATSMQTEVTHDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DC72</accession>
    <name>C5DC72_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0B00528p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0B00528g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the NtaA/SnaA/DszA monooxygenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551821.1">
        <property type="nucleotide sequence ID" value="XM_002551775.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DC72"/>
    <dbReference type="GeneID" id="8290640"/>
    <dbReference type="KEGG" id="lth:KLTH0B00528g"/>
    <dbReference type="eggNOG" id="ENOG502QSR6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022256_0_0_1"/>
    <dbReference type="InParanoid" id="C5DC72"/>
    <dbReference type="OMA" id="SWRGDEI"/>
    <dbReference type="OrthoDB" id="5561043at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="entry name" value="Luciferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051260">
        <property type="entry name" value="Diverse_substr_monoxygenases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016215">
        <property type="entry name" value="NTA_MOA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03860">
        <property type="entry name" value="FMN_nitrolo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30011">
        <property type="entry name" value="ALKANESULFONATE MONOOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30011:SF16">
        <property type="entry name" value="C2H2 FINGER DOMAIN TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000337">
        <property type="entry name" value="NTA_MOA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="Bacterial luciferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2" id="KW-0288">FMN</keyword>
    <keyword evidence="4" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Luciferase-like" evidence="7">
        <location>
            <begin position="58"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00033748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR21382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="514" mass="57241" checksum="F51F58E8C92E83A2" modified="2009-07-28" version="1">MTIDHTAKRSSELEVGNAKRAQLTSPKRKNLIVNAFIMGSAGNQSVGIWKHPQDKSAELYQSPEYWINLAKLLERGKFNAVFLADVLGPYDVYKGPANVGPVAKAGSQWPISDPSYFIPLMAAVTTKLAFGMTISTISEQPYHLARRLGTLDLLTNGRVGWNIVTSYLDSASRNLLDGKNLPPNEERYARAGEYVDVIYKLFLSSWRDESFKADKKTGVFTDPEGLRHINHVGKYFNVPGPAITHPSQQKLPVIIQAGTSAKGKDLSAKNAEVVFLASLTPEALGASIKEIKQIATTKYHRNPSKIKFIGLVTVILGDTHEEAENKYNEYKANYSDEEGAKAMFSGWTGVDIDKFEDDEVLSNVDHIAIASAVKKWQTAYPRVEKWTKRAIIDEIKVGGSGPVLIGTPQEVASEIQNWVDISDIDGFNFAYTVLPQSFEEIIDKLLPELEKLGLFWHDYPNASDSSKYLTFREQLFGEPSLDKTHPASDLRWKADETREAYEARIGEALKKLVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCG2</accession>
    <name>C5DCG2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">General transcription and DNA repair factor IIH</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">KLTH0B02794g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to TFIIK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module TFIIK controls the initiation of transcription.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the GTF2H2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551911.1">
        <property type="nucleotide sequence ID" value="XM_002551865.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCG2"/>
    <dbReference type="FunCoup" id="C5DCG2">
        <property type="interactions" value="1098"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCG2"/>
    <dbReference type="GeneID" id="8290742"/>
    <dbReference type="KEGG" id="lth:KLTH0B02794g"/>
    <dbReference type="eggNOG" id="KOG2807">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028556_2_0_1"/>
    <dbReference type="InParanoid" id="C5DCG2"/>
    <dbReference type="OMA" id="INWVEVP"/>
    <dbReference type="OrthoDB" id="284275at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000439">
        <property type="term" value="C:transcription factor TFIIH core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005675">
        <property type="term" value="C:transcription factor TFIIH holo complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01453">
        <property type="entry name" value="vWA_transcription_factor_IIH_type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000477">
        <property type="entry name" value="General transcription and DNA repair factor IIH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000015">
        <property type="entry name" value="General transcription factor IIH subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007198">
        <property type="entry name" value="Ssl1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004595">
        <property type="entry name" value="TFIIH_C1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012170">
        <property type="entry name" value="TFIIH_SSL1/p44"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00622">
        <property type="entry name" value="ssl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12695">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIH SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12695:SF2">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIH SUBUNIT 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07975">
        <property type="entry name" value="C1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04056">
        <property type="entry name" value="Ssl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015919">
        <property type="entry name" value="TFIIH_SSL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01047">
        <property type="entry name" value="C1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="8 12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="VWFA" evidence="15">
        <location>
            <begin position="134"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="13">
        <location>
            <begin position="358"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="16"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="33"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="47"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR015919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR015919-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAR21473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="470" mass="53027" checksum="39817454B9DC75BE" modified="2009-07-28" version="1">MAPIVESDSEDESLNRPKKQVRGRTSKGKKHVRIEEDYNEDGHSMSEDDENLFSEDEADSGPSNGRKKKKLANKNLQGANGGYAWEEDIQRSWDLVKVDDEGNMAALVSSIIEARKKRASNKNLTPFQRGIIRTMVLVLDCSDAMMEKDLRPNRYAMTIQYAINFVHEFFDQNPISQIGIVCMRNDLAQLVSQVSGNPQDHIDALKAIRKQEPKGNSSLQNALEMARGLLLHVPAHCTREVLIIFGALSSTDPGDIHQTIASLVQERIRVRVIGLSAQVAICKELCKQTNYGDNSFYGVILNEVHFKDLFAEAVTPLPVNKINKGFTLVKMGFPTRVYEDMPSFCTCHSKLMYGGYFCPNCKSKVCSLPTVCPCCDLMLILSTHLARSYHHLMPLKTFQEVDVAETFPTDTCYSCQKRFPRLKNQKTQELLTSSRYRCADCHQDFCIDCDVFIHEILHNCPGCESQPGIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCJ2</accession>
    <name>C5DCJ2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0B03498p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0B03498g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551941.1">
        <property type="nucleotide sequence ID" value="XM_002551895.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCJ2"/>
    <dbReference type="FunCoup" id="C5DCJ2">
        <property type="interactions" value="47"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCJ2"/>
    <dbReference type="GeneID" id="8290774"/>
    <dbReference type="KEGG" id="lth:KLTH0B03498g"/>
    <dbReference type="eggNOG" id="KOG0748">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025943_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCJ2"/>
    <dbReference type="OMA" id="CSHTLWH"/>
    <dbReference type="OrthoDB" id="5585746at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006882">
        <property type="term" value="P:intracellular zinc ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004254">
        <property type="entry name" value="AdipoR/HlyIII-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855:SF97">
        <property type="entry name" value="ADIPOR-LIKE RECEPTOR IZH3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855">
        <property type="entry name" value="ADIPOR/PROGESTIN RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03006">
        <property type="entry name" value="HlyIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="258"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="295"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="329"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="363"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="396"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="427"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="509"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="315"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR604254-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR21503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="546" mass="61374" checksum="35CAD15BAE60DA19" modified="2009-07-28" version="1">MDRLESLLPNDGAYRPRLHKLRTRGKQIVRWSKSQVAGVPAYALRKITWGAASEADEDAGDDARAAAAEPPTELFAADFDAITMVDSRPQSMLNSYRMKDAQTARWHAKTATAAAAMASGVSYVSSAATLVSSSSFSSAENISLSLPSASPARSLSSADSCGTSPVSPVAGLRICDAKAIEKSFQSIQNDAEAVAASIKFHNYEVALQLGQQRHLHYYELPFPWRENRFIIHGYRFYNSYSKSLLSIFNWYGWHNETVNIWTHLCGALYFAFLIFKGFSQTTVYQSELVPTAAKFMAFVFLTAGLECFLFSVVWHTFNGICHLKQRSNCACVDYTGITVLVTASILTTEFVTLSGSSDATFTWSLLFFTGASAALGAVGVFMNWSPKFDRPESRPLRIAFYLLLASFGFFSYLHSRLFNTAVDSSDLIKPVFMKSLAWYIIGVVFYGTFIPERWRSDVQVDANIPTEEQLTTDLEIITKHRHIHFRSQPASHHKCKNADHKRSFKSLWWVDYFCCSHTLWHLFVLLGVIGHYTAMLEMFEKRWLRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCS5</accession>
    <name>C5DCS5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0B05434p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0B05434g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552024.1">
        <property type="nucleotide sequence ID" value="XM_002551978.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCS5"/>
    <dbReference type="FunCoup" id="C5DCS5">
        <property type="interactions" value="53"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCS5"/>
    <dbReference type="GeneID" id="8290862"/>
    <dbReference type="KEGG" id="lth:KLTH0B05434g"/>
    <dbReference type="eggNOG" id="KOG3308">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058668_1_1_1"/>
    <dbReference type="InParanoid" id="C5DCS5"/>
    <dbReference type="OMA" id="MDMEAMT"/>
    <dbReference type="OrthoDB" id="10041966at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02024">
        <property type="entry name" value="NRK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006083">
        <property type="entry name" value="PRK/URK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10285">
        <property type="entry name" value="URIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00485">
        <property type="entry name" value="PRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphoribulokinase/uridine kinase" evidence="1">
        <location>
            <begin position="9"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="239" mass="26923" checksum="847FC66F4C7CC34B" modified="2009-07-28" version="1">MESPQVLLVGISGCSSSGKTTLAKLSAELLPTAVLIHQDDFFKHDEDIPFNDKYQIRDWDSPEALDMNLFGKELDHIKKTGSVSTTLIHNNNHDNSGAAEVRPETQKEVKTKFAKAFEGTNTKVVFVDGFMMFNDQNIKSKFDLKLLVRAPYGTLKKRRAARSGYQTLDSFWIDPPYYFDEFVYSSYARSHSQLFEDGNVEGVLKKGSGILDFINDDSTDINDALLWVCNCVVSMAQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCS6</accession>
    <name>C5DCS6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0B05456p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0B05456g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MIP18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552025.1">
        <property type="nucleotide sequence ID" value="XM_002551979.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCS6"/>
    <dbReference type="FunCoup" id="C5DCS6">
        <property type="interactions" value="440"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCS6"/>
    <dbReference type="GeneID" id="8290863"/>
    <dbReference type="KEGG" id="lth:KLTH0B05456g"/>
    <dbReference type="eggNOG" id="KOG3381">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075876_1_1_1"/>
    <dbReference type="InParanoid" id="C5DCS6"/>
    <dbReference type="OMA" id="YDMIAHI"/>
    <dbReference type="OrthoDB" id="2746at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.130:FF:000007">
        <property type="entry name" value="Cytoplasmic protein required for cell viability"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="entry name" value="Fe-S cluster assembly (FSCA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039796">
        <property type="entry name" value="MIP18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002744">
        <property type="entry name" value="MIP18-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12377:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR ASSEMBLY COMPONENT 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12377">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR ASSEMBLY COMPONENT 2B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01883">
        <property type="entry name" value="FeS_assembly_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="Fe-S cluster assembly (FSCA) domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIP18 family-like" evidence="4">
        <location>
            <begin position="98"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="82"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR21587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="222" mass="24953" checksum="1E3BD050DF405ECA" modified="2009-07-28" version="1">MSEFINENPDILDENLLPSRKEEEGDAFQDEALNTVNLLRRKRLLKIHQTTACKVYRNVELIDRLKAQEMALDQVPDLTASETEESDEGEPEDPIDAQEIYDMIAHISDPEHPLTLGQLAVVNLPDIEVSESNDKSKISEVLVKITPTITHCSLATLIGLGIRVRLERSLPSRYRITILLKEGSHQSENQVNKQLNDKERVAAACENDQLLGVVSKMLSTCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCV4</accession>
    <name>C5DCV4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">mRNA stability protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0B06094g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Plays an essential role in initiation of the G0 program by preventing the degradation of specific nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the endosulfine family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552053.1">
        <property type="nucleotide sequence ID" value="XM_002552007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCV4"/>
    <dbReference type="FunCoup" id="C5DCV4">
        <property type="interactions" value="28"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCV4"/>
    <dbReference type="GeneID" id="8290892"/>
    <dbReference type="KEGG" id="lth:KLTH0B06094g"/>
    <dbReference type="eggNOG" id="KOG4076">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_135184_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCV4"/>
    <dbReference type="OMA" id="MQERKYF"/>
    <dbReference type="OrthoDB" id="5949865at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006760">
        <property type="entry name" value="Endosulphine"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10358">
        <property type="entry name" value="ENDOSULFINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10358:SF6">
        <property type="entry name" value="ENDOSULFINE, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04667">
        <property type="entry name" value="Endosulfine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="70"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="75"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR21615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="129" mass="14048" checksum="49EDD48F0E3AA08D" modified="2009-07-28" version="1">MSKGNDLSPTSSNVDVKEGVDASKLTPQEAKLLKMYGRLPSKKDLFKHKLQERKYFDSGDYALNKAGVKSDDLQSDSIGNNHLPVTNPSGLRESIIKRRLSSSAGSVDAHDLRRQGSISSGPPPRSPNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>C5DD41</accession>
    <name>C5DD41_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Mannosyltransferase</fullName>
            <ecNumber evidence="10">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0B08096g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Endoplasmic reticulum membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the glycosyltransferase 22 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="10"/>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552140.1">
        <property type="nucleotide sequence ID" value="XM_002552094.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DD41"/>
    <dbReference type="FunCoup" id="C5DD41">
        <property type="interactions" value="993"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DD41"/>
    <dbReference type="GeneID" id="8290980"/>
    <dbReference type="KEGG" id="lth:KLTH0B08096g"/>
    <dbReference type="eggNOG" id="KOG2515">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018152_1_1_1"/>
    <dbReference type="InParanoid" id="C5DD41"/>
    <dbReference type="OMA" id="PRDMHAK"/>
    <dbReference type="OrthoDB" id="497541at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005599">
        <property type="entry name" value="GPI_mannosylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760:SF2">
        <property type="entry name" value="ALPHA-1,2-MANNOSYLTRANSFERASE ALG9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03901">
        <property type="entry name" value="Glyco_transf_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002947773" description="Mannosyltransferase" evidence="11">
        <location>
            <begin position="27"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="93"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="177"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="268"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="296"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="320"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="352"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR21702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="551" mass="63256" checksum="1AF81BA65869A683" modified="2009-07-28" version="1">MNSNWTSVILFGAFVACRFYLQPTYSLISDCDETFNYWEPLNFLLRGFGKQTWEYSPEYSIRSWAFLIPLYSVLNPSMRILEAFHLPMFNLFYIARFFLGLFSLVMEWLFFEEIKSTLSTQTATFWLFFQVFNPGWFHASVELLPSSFAMISLLGSLKFTLRYLSTGAQDAFVKALLFNFVGGILGWPFVMILSVPLVVHYIFSHRILNTLKAGISAASRLAVVTAVVFAVDSMFYGKFTPVAWNIVMYNVIGANEKSGPNIFGVEPWYYYIFSLLLNIPLPVLLGSIVGLSNLRLWPVWGALATWLTVFLLQPHKEERFLYPIYGFVTLASSVGFSSFWRKWSGSKLTRNTVFLILLCSTASQALLRMFALVQNYKAPLDVYSHLPEASGPQTVCVGREWFHFPASMFLPDNYRLGFVPSGFDGLLPGDFKETDSILNSIRDKPVGMNNQNLFDSSKLVVTEQCDYFVDFMLHSDNEKDAFDPTNLPQGWKKLYSALFVDAPESKFFGRALLLPEGLTRLIPQQLQSRWSKIYQVKYLEYCIFERHREEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DD53</accession>
    <name>C5DD53_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0B08382p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0B08382g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552152.1">
        <property type="nucleotide sequence ID" value="XM_002552106.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DD53"/>
    <dbReference type="FunCoup" id="C5DD53">
        <property type="interactions" value="111"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DD53"/>
    <dbReference type="GeneID" id="8290992"/>
    <dbReference type="KEGG" id="lth:KLTH0B08382g"/>
    <dbReference type="eggNOG" id="KOG2821">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062289_0_0_1"/>
    <dbReference type="InParanoid" id="C5DD53"/>
    <dbReference type="OMA" id="RMLYFKY"/>
    <dbReference type="OrthoDB" id="21513at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070449">
        <property type="term" value="C:elongin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051870">
        <property type="entry name" value="Elongin-A_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010684">
        <property type="entry name" value="RNA_pol_II_trans_fac_SIII_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15141">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR B POLYPEPTIDE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15141:SF76">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR B POLYPEPTIDE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06881">
        <property type="entry name" value="Elongin_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="242"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="156"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="249"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="353"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR21714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="377" mass="43298" checksum="31FCCBEC2C6B81F7" modified="2009-07-28" version="1">MPISENEEEFTMEHKQTKIKSLSSLCEISLMRNHLMLQNVANVPYRLVRNVLLKLKMEHLCKLEESNVLLIFEDEEAWVELLKKDFPMHVHDTYHRKRDEIVAFYADFVEKHDPPKLRDEELMRGFLRFAVRKEPATHKYKVPSRMLYFKYQEDMLRKQELSTQRLRMRMQEMQQEKEKKQIVPLDDPVYCERRTKAAARAGAGERSGLFAKSYKEHQKRQLHFKSGGYDAKSRPVKRLAFGGQVGRPAPSPAAPAPCAAPAPPVAAPAPLPATMQDTSQPKRSTPAVAPSARRKPTPEQNLFLKRRRPLPKPKTPPTPRAEPSVKRKPTVVKVNSTKIHRKNTKTSIFAAPSPQQQVLSQHSNTSSAYIFDQPRQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDA0</accession>
    <name>C5DDA0_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Iron-sulfur clusters transporter ATM1, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Iron-sulfur clusters transporter atm1, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ordered locus">KLTH0B09482g</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Performs an essential function in the generation of cytoplasmic iron-sulfur proteins by mediating the ATP-dependent export of Fe/S cluster precursors synthesized by NFS1 and other mitochondrial proteins. Hydrolyzes ATP. Binds glutathione and may function by transporting a glutathione-conjugated iron-sulfur compound.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the ABC transporter superfamily. ABCB family. Heavy Metal importer (TC 3.A.1.210) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552199.1">
        <property type="nucleotide sequence ID" value="XM_002552153.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDA0"/>
    <dbReference type="FunCoup" id="C5DDA0">
        <property type="interactions" value="702"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDA0"/>
    <dbReference type="GeneID" id="8291042"/>
    <dbReference type="KEGG" id="lth:KLTH0B09482g"/>
    <dbReference type="eggNOG" id="KOG0057">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_84_1_1"/>
    <dbReference type="InParanoid" id="C5DDA0"/>
    <dbReference type="OMA" id="VFHIIPI"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140466">
        <property type="term" value="P:iron-sulfur cluster export from the mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18582">
        <property type="entry name" value="ABC_6TM_ATM1_ABCB7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03253">
        <property type="entry name" value="ABCC_ATM1_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000004">
        <property type="entry name" value="ATP-binding cassette sub-family B member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000287">
        <property type="entry name" value="Multidrug ABC transporter ATP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221:SF402">
        <property type="entry name" value="IRON-SULFUR CLUSTERS TRANSPORTER ABCB7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="110"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="151"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="224"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="257"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="347"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="20">
        <location>
            <begin position="111"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="19">
        <location>
            <begin position="435"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="58"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="58"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00040792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00045666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="CAR21761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="694" mass="77007" checksum="8B0AC82D918192F7" modified="2009-07-28" version="1">MRPVDSIKLGGLVFRSLKPLPVRTSLCSRAFSLSPTARVSSTIPTINSLRLYSTKNSPKIQENTVKSSPAPAKPKANEASKTPTIGELKILKDLFKYIWPNGDNKVKARVLIALSLLVGSKVLNVQVPFFFKQIVDSMNIEWADPTAVLPIAVTAMILSYGAARFGSVLFGELRNAIFASVAQSAITRVSLQTFQHLMKLDLGWHLSRQTGGLTRAMDRGTKGISYVLSAMVFHIIPITVEIGAVCGILTYQFGASFAAMTFTTMLLYSIFTFKTTAWRTEFRKSANKADNKAATVAVDSLINFEAVKYFNNEQFLANKYHNSLAQYRDSQIKVAQSLAFLNAGQNFIFTSALTAMMYMGCTGVISGTLSVGDLVLINQLVFQLSVPLNFLGSVYRELKQSLIDMESLFQLQRNKVKIQNKEKPLMLSSQRPSKIVFENVSFGYDPDRMILKNSSFTMPAGWKTAIVGPSGSGKSTILKLVFRFYDPQEGRILVDGKDVRELDIESLRRAIGVVPQDTPLFNDSIWENVRFGKISASDEEIRLAVEKAQLESLVDQLPKGRDTIVGERGLMISGGEKQRLAIARVLLKDAPIMFFDEATSALDTHTEQALLRTIKDNFPTGSKTSVYIAHRLRTIADADKIIVIQQGQVKEEGKHAELLAKPGSLYAELWNVQENLDVLNEELQREETQEKGDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDB4</accession>
    <name>C5DDB4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">1,3-beta-glucanosyltransferase</fullName>
            <ecNumber evidence="11">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0B09812g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Splits internally a 1,3-beta-glucan molecule and transfers the newly generated reducing end (the donor) to the non-reducing end of another 1,3-beta-glucan molecule (the acceptor) forming a 1,3-beta linkage, resulting in the elongation of 1,3-beta-glucan chains in the cell wall.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell envelope</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Cell membrane</location>
            <topology evidence="11">Lipid-anchor</topology>
            <topology evidence="11">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the glycosyl hydrolase 72 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="11"/>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552213.1">
        <property type="nucleotide sequence ID" value="XM_002552167.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDB4"/>
    <dbReference type="STRING" id="559295.C5DDB4"/>
    <dbReference type="GeneID" id="8291057"/>
    <dbReference type="KEGG" id="lth:KLTH0B09812g"/>
    <dbReference type="eggNOG" id="ENOG502QPST">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021855_2_1_1"/>
    <dbReference type="InParanoid" id="C5DDB4"/>
    <dbReference type="OMA" id="PAPCENC"/>
    <dbReference type="OrthoDB" id="421038at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042124">
        <property type="term" value="F:1,3-beta-glucanosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071970">
        <property type="term" value="P:fungal-type cell wall (1-&gt;3)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000038">
        <property type="entry name" value="1,3-beta-glucanosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004886">
        <property type="entry name" value="Glucanosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012946">
        <property type="entry name" value="X8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31468">
        <property type="entry name" value="1,3-BETA-GLUCANOSYLTRANSFERASE GAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31468:SF2">
        <property type="entry name" value="1,3-BETA-GLUCANOSYLTRANSFERASE GAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03198">
        <property type="entry name" value="Glyco_hydro_72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07983">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00768">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 11" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="9 11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005125147" description="1,3-beta-glucanosyltransferase" evidence="11">
        <location>
            <begin position="22"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="domain" description="X8" evidence="13">
        <location>
            <begin position="381"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="477"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="477"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR21775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="543" mass="58222" checksum="062D2B1A77862382" modified="2009-07-28" version="1">MRFATSSLLLSLLSVLQLALADGNDTTPAIEVKGNKFFFSNNGSQFYMKGVAYQADTANSTAGDEDTIVDPLADYETCSRDLPYLLGVHTNVVRVYALNTSLDHSKCMNAFADAGIYVIADLSEPSESINRDSPAWTLDLYERYTSVVDAVANYSNVLGFFAGNEVTNNKSNTNASPFVKAAIRDTKKYMKDKGYRNIPVGYSSNDDEETRVSMADYFACGDDDVKADFYGINMYEWCGSSTFQSSGYEDRTKEFSNLSIPAFFSEYGCNESPPRKFQEVEALYGDDMTDVWSGGIVYMYFEEANKYGLVSTSGNTVKTLDDYNNLKKEIASVSPSSVNKNSYTPSTTSLKCPATGTNWNASTELPPTPDKDVCDCLKSSLSCVLADDVESKDYGDLFSYLCDKIGCDEITADGSAGNYGEYSFCDADIKLSFLLDKYYQKNGKNKSDCSFSGSASLASSTSQASSCQAKLSSATASAKSTGSSNSNSSGSSASSSNSSSSASSSSNSKKSGGIQVKPASPAGLWLVAIMVASASVGLGSIVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDC8</accession>
    <name>C5DDC8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphate transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0B10186g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Sodium-phosphate symporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552227.1">
        <property type="nucleotide sequence ID" value="XM_002552181.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDC8"/>
    <dbReference type="FunCoup" id="C5DDC8">
        <property type="interactions" value="1077"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDC8"/>
    <dbReference type="GeneID" id="8291074"/>
    <dbReference type="KEGG" id="lth:KLTH0B10186g"/>
    <dbReference type="eggNOG" id="KOG2493">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015355_3_0_1"/>
    <dbReference type="InParanoid" id="C5DDC8"/>
    <dbReference type="OMA" id="MQAFCIA"/>
    <dbReference type="OrthoDB" id="260807at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001204">
        <property type="entry name" value="Phos_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11101">
        <property type="entry name" value="PHOSPHATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11101:SF80">
        <property type="entry name" value="PHOSPHATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01384">
        <property type="entry name" value="PHO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="118"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="145"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="184"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="225"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="403"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="450"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="537"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="301"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR21789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="576" mass="62779" checksum="1A27A27602533506" modified="2009-07-28" version="1">MTLHQFDYIFAIAMLFAFLDAFNIGANDVANSFASAISSKSLKYWQAMILAGLCEFLGAVLAGSRVSKTIKNDIIDASTFNNDPAVLMLTMTCALVGSSCWLTIATSIGLPVSNTHSIVGGTIGAGIAASGAGGVVWGWDGVAQIIASWFIAPVLAGLIAAIIFTISRICVLEVKTLEKSIKNALLLVGLLVFATFSILTMLIVWKGSPNLHLDNLSETETALSIVLTGAIASVVYFIFFYPFYRRKLLHQDWTLKLADILRGPVFYFKPLDEIPPMPENHQLTIDYYEGRRFPQETAVGVSDEENKMPNVSTKSINDNDDNLQRTGSVETKTEPENKMSTRQYWWSLLKQGPKQWPKLLWLVVSHGWTQDVVSAQVNDKDMLSGNLVDMFKRSKYYDNRVEYIYSVLQAITAATMSFAHGANDVANATGPLAVVYEIWKTNATAAKSDVPVWVLAYGGAALVLGCWTYGYNIIKNLGNKIILQSPSRGFSIELAAAITTVMATQLAIPTSTTQIAVGGIVAVGLCNKDVKSVNWKMVAWCYSGWFLTLPIAGLIAGILNGIILNAPHFGGVYEMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDL1</accession>
    <name>C5DDL1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0C01870p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0C01870g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat PROPPIN family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552310.1">
        <property type="nucleotide sequence ID" value="XM_002552264.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDL1"/>
    <dbReference type="FunCoup" id="C5DDL1">
        <property type="interactions" value="409"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDL1"/>
    <dbReference type="GeneID" id="8291169"/>
    <dbReference type="KEGG" id="lth:KLTH0C01870g"/>
    <dbReference type="eggNOG" id="KOG2111">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025895_0_1_1"/>
    <dbReference type="InParanoid" id="C5DDL1"/>
    <dbReference type="OMA" id="GGPQCMC"/>
    <dbReference type="OrthoDB" id="1667587at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048720">
        <property type="entry name" value="PROPPIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11227">
        <property type="entry name" value="WD-REPEAT PROTEIN INTERACTING WITH PHOSPHOINOSIDES WIPI -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21032">
        <property type="entry name" value="PROPPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR21872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="423" mass="47762" checksum="F9264FD63159D274" modified="2009-07-28" version="1">MNTRPPIVASNPVSDGRFLGVNFNQDYSCFSCSTQTGFMVFNVDPLECKLSRQFSNGNASGIATTRMLYRTNYVALVGGGRKPRYPPNKLVIWDDLQQRESITLSFMSAVKEMFLSRVHIVVVLENSIEIYEFSASHKRLISPLETCAGAAADFVVCQRTMRRLSATQAQASSGNIPQTITKGILAFPSARNPGQVQVADLSHLQSSEIEERAATQLPTSIIKAHKTPIRLIKLSPNGSMVATCSQQGTIIRIFSTQNGSLLGEFRRGLDRADLYEMAWSPRSNRLAVVSDKQTLHIFQVTDEDGDMKNKTHVLKDVPFFWKPKYLDSTWSMCSLHLRSALKGRDSMNDQDFYNDRCKLGWCQEGDEDSLVLIWKQSGVWEKYAILEKEPKTYAVNETLQTSVTAQNQNKKHWEIVRESWRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDP9</accession>
    <name>C5DDP9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0C02772p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0C02772g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552348.1">
        <property type="nucleotide sequence ID" value="XM_002552302.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDP9"/>
    <dbReference type="GeneID" id="8291209"/>
    <dbReference type="KEGG" id="lth:KLTH0C02772g"/>
    <dbReference type="HOGENOM" id="CLU_1635700_0_0_1"/>
    <dbReference type="InParanoid" id="C5DDP9"/>
    <dbReference type="OMA" id="VNYCLYD"/>
    <dbReference type="OrthoDB" id="4034278at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="162" mass="17646" checksum="602362A8FAA71EC3" modified="2009-07-28" version="1">MVPQFGTGFNGDLGNTLKRDLQHGSDGPQQPTRKRLKASMGSFPGRAAPAENTREAPPLIDDAVCAIAMSARQNSSDSATSSPYVEIEDYILEGYGSALINGSYNSRLSLDNITAVNYCLYDKDLEEFDDSDNGTSDGKTLVETPESQDVDMDMDMDMNMDR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2010-10-05" modified="2025-06-18" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDQ4</accession>
    <name>AIM32_LACTC</name>
    <protein>
        <recommendedName>
            <fullName>Altered inheritance of mitochondria protein 32</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">AIM32</name>
        <name type="ordered locus">KLTH0C02882g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AIM32 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552353.1">
        <property type="nucleotide sequence ID" value="XM_002552307.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDQ4"/>
    <dbReference type="SMR" id="C5DDQ4"/>
    <dbReference type="FunCoup" id="C5DDQ4">
        <property type="interactions" value="30"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDQ4"/>
    <dbReference type="GeneID" id="8291214"/>
    <dbReference type="KEGG" id="lth:KLTH0C02882g"/>
    <dbReference type="eggNOG" id="ENOG502QS3W">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044499_1_0_1"/>
    <dbReference type="InParanoid" id="C5DDQ4"/>
    <dbReference type="OMA" id="IPEMKIY"/>
    <dbReference type="OrthoDB" id="10253744at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="CDD" id="cd03062">
        <property type="entry name" value="TRX_Fd_Sucrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009737">
        <property type="entry name" value="Aim32/Apd1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31902">
        <property type="entry name" value="ACTIN PATCHES DISTAL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31902:SF7">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06999">
        <property type="entry name" value="Suc_Fer-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000399698" description="Altered inheritance of mitochondria protein 32">
        <location>
            <begin position="1"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="332" mass="37286" checksum="4D70A35C27A60472" modified="2009-07-28" version="1">MFRVRAPWLKTLVELRQGTTQRRALHSKYRHVGFGASAQLQQSCECFIQKLNRELPRDAQLDASIDLPKKTPSYHKHLMLISPPDEAGKDPEWKGSWQAKLELNPEWPYSAIAEVKKHLKETKRGEGILINAISVMRSKSLGDPSSEKKARFLALPDMKVYEVSQSELKDFAYFVGEGMVQQPRGRAVAFADYLKGADAISKELEHREVASTAAAGMKQFAAQSYQNNLVLVCGHHQRDERCGQIAPRLIKEFEAKVEEDLDLAIVSHIGGHKFAGNVIFYKFLGFEADGKATVDSLWFGKILPSAVPTLLEHLGRNEIITPWFRGGCLLES</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDQ6</accession>
    <name>C5DDQ6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0C02926p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0C02926g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552355.1">
        <property type="nucleotide sequence ID" value="XM_002552309.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDQ6"/>
    <dbReference type="FunCoup" id="C5DDQ6">
        <property type="interactions" value="154"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDQ6"/>
    <dbReference type="GeneID" id="8291216"/>
    <dbReference type="KEGG" id="lth:KLTH0C02926g"/>
    <dbReference type="eggNOG" id="ENOG502QT03">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_719597_0_0_1"/>
    <dbReference type="InParanoid" id="C5DDQ6"/>
    <dbReference type="OMA" id="IMLAWLY"/>
    <dbReference type="OrthoDB" id="4076669at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022757">
        <property type="entry name" value="Gsf2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11055">
        <property type="entry name" value="Gsf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="179"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="385" mass="44318" checksum="829EE570D8F63B34" modified="2009-07-28" version="1">MEICVRINDDCENDYTFQVSKEDTFESKIMKVFDKNEGLSRFMVLRPSIFYKKQPTGLSKSMHPGFLTENGCLLFHYDADERKYREPLELKNKKIWEQMWPGQLVLPEWELSYRNIAVFAAILLGWLYTDLPDVISPTPGICFTNQLSKRIMVVARWYGYNAIADKLAEEVQVNSAGVVAQWLFFALHFVKVIFVALVFYSGLVNPVSFNFLDFYSTRKVVITKNADKLKETLRSVGWIGAKRATYDEYRDTYYNYRLEKAGGLVPAYKSGIMQVAANPGVNLEKGEGFQTPLDKRFTESTFKTMEKSRKFVLSEEYLIQLEKDLKEVIKKCEGDVPAINQQIRKFRRLGLFESGPELTRLVELRKEVAPEPETGAADADAKKTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDX5</accession>
    <name>C5DDX5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0C04554p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0C04554g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the THADA family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552424.1">
        <property type="nucleotide sequence ID" value="XM_002552378.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DDX5">
        <property type="interactions" value="38"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDX5"/>
    <dbReference type="GeneID" id="8291289"/>
    <dbReference type="KEGG" id="lth:KLTH0C04554g"/>
    <dbReference type="eggNOG" id="KOG1810">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001011_2_0_1"/>
    <dbReference type="InParanoid" id="C5DDX5"/>
    <dbReference type="OMA" id="TQHITRR"/>
    <dbReference type="OrthoDB" id="73997at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056843">
        <property type="entry name" value="THADA-like_TPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056842">
        <property type="entry name" value="THADA-like_TPR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019442">
        <property type="entry name" value="THADA/TRM732_DUF2428"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051954">
        <property type="entry name" value="tRNA_methyltransferase_THADA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14387:SF0">
        <property type="entry name" value="DUF2428 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14387">
        <property type="entry name" value="THADA/DEATH RECEPTOR INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10350">
        <property type="entry name" value="DUF2428"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25150">
        <property type="entry name" value="TPR_Trm732"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25151">
        <property type="entry name" value="TPR_Trm732_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA (32-2'-O)-methyltransferase regulator THADA-like TPR repeats region" evidence="4">
        <location>
            <begin position="229"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2428" evidence="3">
        <location>
            <begin position="605"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA (32-2'-O)-methyltransferase regulator THADA-like C-terminal TPR repeats region" evidence="5">
        <location>
            <begin position="831"/>
            <end position="986"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR21986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1426" mass="161251" checksum="6D35F70D0479178D" modified="2009-07-28" version="1">MSNEDGCKAIKQFLIGLKPQQVKNDTTISEKLCSCFISVITALRNENAINDSERLVLTDSISIWFLRSRQLLEMEQDQQGCSPFLQKLRTDILTIENCTFLFHYVIDYWNEGGAALANSLSDLFTKLLQLMKLVHSTAVFQETLDGWLSQVLDIPASMRVLYYLLDVFASETSMYKVLQSKPNFVADSLGLMWTESLATPIGKCVTSVLLNVYEGHYRKCKRREDLEEWFLLWEGPTLRYFQDPRISKRIEICILVPIFKSLSNNIFGQFVNRNFESEAPALIPLLKIGQELSIEEEPFDGDKLVSLKFVEELLQQDAYKLSAFELLTFSAKKSKVIAPYIYDVIKRNIHVFFVDIEVRTRNAFHSVLKHFIDRVRDSSYSLNRDFISLHAKNKFPDEQAQKLQQIDDAIDFLQWLTGFLKTQLLPGSQYQRKILSTKILKTLASSSIDPSIGQPYLDPRLRTPFPFSCKLSEDETLQRLLIDNLTDNYNDIRENCLQLLTMFAYSKTPTGTENLEAVSRKSWELLKSYKNCDGGAKLIEFLVEISDDKSTLVYVLVKELQKKIIKSGASFKEAIKTPISGYFLALSYILQKSDAIPETVTLRSIIDECISLMNNNWKVVEFALCDDPFEKNTQTQHDICEVSDPLVISYAFRSIKESAGLLAALIRLPGLTIKQLTACGELMLAQMSTIRHSGAFQSLIPSFAACCRRSNTDIPQQLQVWLEEILNRLETKTQFITRRSGGLPHIITSILGAEDNKNRPLLKVTFDKLFSIASLPVSEHEEQVDLPQVNAFNCIKALFVESSLSAACSPYVYPSLVLCLQSFTSPLWSIRNCSYMLFSALQNRLFGKAGKSVSARLFFSRFSGIREILLDQFKSSVKNSLCYQDTAEGSSLSSGMDSQIVSIFLVMTILSRLKQTPGFEGLKEFESLVIKCLGSHNWTLRQLAARTLPSMSDNTIDLAKLLLKKLTLASTNQNMMHGCILAVSELIKITMESSNSCSLPDELSQLVERGISRAITSNNCFVTANAYVGLVELLYSSGSISKTCVSSWLPKLGEHFVKLNTSYSVDGTRQLCLRSLLLFLLRHENEDNLQDVLLLGLYSPYFKVQIAAAQYICTNDKAAHVKTNAIADRLLEILFEDNAWDYVKLSVLRSLIVLEKEVDTSALMKLVISARNDELRAVALEYLGFFVDKNNSQYDYYVKHFSRDQSPFELRKSALKSLINFSKRNSDIRASFLISRFLYDDDQELRMLSASFINQCVLGLKGFRQSTTSAVTADLFFDTMESYPQLPNGFDTVILEALQRDVGTISFQKYVGAGKEDLFEVEPENQFRNTIEEASRFIGVLKCFFKESSSVQAYISTLLDELIAQISDVGISDAPLGWGSDFRIFTQIVLARKIAIEFGVEGLQKLDKLLIAVNCNPLIFEITSLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>C5DE69</accession>
    <name>C5DE69_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="6">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0C06732g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="6"/>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552518.1">
        <property type="nucleotide sequence ID" value="XM_002552472.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DE69"/>
    <dbReference type="FunCoup" id="C5DE69">
        <property type="interactions" value="289"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DE69"/>
    <dbReference type="GeneID" id="8291386"/>
    <dbReference type="KEGG" id="lth:KLTH0C06732g"/>
    <dbReference type="eggNOG" id="KOG1864">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008279_12_1_1"/>
    <dbReference type="InParanoid" id="C5DE69"/>
    <dbReference type="OMA" id="PMHGHYV"/>
    <dbReference type="OrthoDB" id="27652at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000131">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF733">
        <property type="entry name" value="RE52890P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 6" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="8">
        <location>
            <begin position="143"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="178"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="283"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="668"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="58"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="205"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="294"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="321"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="705"/>
            <end position="714"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR22080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="714" mass="79203" checksum="8ECE6A4EC892CB64" modified="2009-07-28" version="1">MSLRKWLTRSDKPSRHKPATQGLHGEIDSAAKDTQAGGAKGDGAELSLGGKILSMITPSDGNSSSSSGPDGVTENGYPSGNRETGSHIHGSQDASDPVSEKAYHSSSAISDFEDLESEYDYFPLIKPSVTPLLPYGDGSNKVFGYENFGNTCYCNSVLQVIYNLPELRVNLLEFPERPSSLPRRRKSEMPGIKPRIFDDTSFTHSNNGSENAKKNSSSSGSAGSLGKEPSRKTSASSLKPGMSARAQRLHNASVVHGTVMASDAITEKLHEGFTRIVVGRVNGKSQKRSVQRTPPSNSASSSSSSVMESSPPNISLASSTRPEETSSEERKSSALIRGPVLNVDHSLIDYLPQGEKPSLYTALKDLYESITENKFLTGVVSPIQFVETLKRENILFSSTMHQDAHEFLNFLLNDISDYINAHSKNNIQAHETTNPMDDNHLNFVDRLFKGTLTNRTKCLTCDNMTYRNEPFLDFAIEVQDDGETDIQTTLADYHQKELLNGANKFYCDECCGLQEAERVVGLKQLPFYLALHMKRFKYSEEQNCNVKLFNRIRYPLDLKVCSTFDASVCKQYELVGLVVHMGGGPHHGHYVSLCKNERFGWLLFDDETVETVNESTVLKFVGNSDDLTTAYLLFYREKTAGATSGNDKSQEYAECVDELIKLDEKIRVGSESGKNDQNTTLEEVPEERRGERKLSSKGLKRRSRIFSFKRSPKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEB4</accession>
    <name>C5DEB4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RP41</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">S18</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">YS12</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0C07810g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the universal ribosomal protein uS17 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552563.1">
        <property type="nucleotide sequence ID" value="XM_002552517.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEB4"/>
    <dbReference type="FunCoup" id="C5DEB4">
        <property type="interactions" value="1273"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEB4"/>
    <dbReference type="GeneID" id="8291435"/>
    <dbReference type="KEGG" id="lth:KLTH0C07810g"/>
    <dbReference type="eggNOG" id="KOG1728">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073626_0_2_1"/>
    <dbReference type="InParanoid" id="C5DEB4"/>
    <dbReference type="OMA" id="DYEKCPF"/>
    <dbReference type="OrthoDB" id="10254436at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00364">
        <property type="entry name" value="Ribosomal_uS17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.1000:FF:000001">
        <property type="entry name" value="40S ribosomal protein S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000266">
        <property type="entry name" value="Ribosomal_uS17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028333">
        <property type="entry name" value="Ribosomal_uS17_arc/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019979">
        <property type="entry name" value="Ribosomal_uS17_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032440">
        <property type="entry name" value="Ribosomal_uS17_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006345">
        <property type="entry name" value="PRK08572.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03630">
        <property type="entry name" value="uS17_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10744">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S11 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10744:SF9">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00366">
        <property type="entry name" value="Ribosomal_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16205">
        <property type="entry name" value="Ribosomal_S17_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00973">
        <property type="entry name" value="RIBOSOMALS17"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00056">
        <property type="entry name" value="RIBOSOMAL_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS17 N-terminal" evidence="9">
        <location>
            <begin position="8"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00075870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00077551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00080769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR22125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="156" mass="17809" checksum="60C60A76946204DD" modified="2009-07-28" version="1">MSTELTVQAERAFQKQPHIFTNPKTKANRRTKRWYKNVGLGFKTPKTAIEGSYIDKKCPFTGNVSIRGKILSGVVVSAKMHRTIVIRRDYLHYVPKYNRYEKRHKNVPAHVSPAFRVEVGDVVTVGQCRPISKTVRFNVLKVTSTAGQANKQFSKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEI5</accession>
    <name>C5DEI5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0C09504p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0C09504g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552634.1">
        <property type="nucleotide sequence ID" value="XM_002552588.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEI5"/>
    <dbReference type="FunCoup" id="C5DEI5">
        <property type="interactions" value="284"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEI5"/>
    <dbReference type="GeneID" id="8291512"/>
    <dbReference type="KEGG" id="lth:KLTH0C09504g"/>
    <dbReference type="eggNOG" id="KOG0748">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023075_2_0_1"/>
    <dbReference type="InParanoid" id="C5DEI5"/>
    <dbReference type="OMA" id="HSQPCPD"/>
    <dbReference type="OrthoDB" id="529367at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006882">
        <property type="term" value="P:intracellular zinc ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004254">
        <property type="entry name" value="AdipoR/HlyIII-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855:SF95">
        <property type="entry name" value="ADIPOR-LIKE RECEPTOR IZH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855">
        <property type="entry name" value="ADIPOR/PROGESTIN RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03006">
        <property type="entry name" value="HlyIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="78"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="113"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="151"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="177"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="208"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="241"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="281"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="279"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR604254-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR22196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="319" mass="35999" checksum="1A2D0BD7B2712E3C" modified="2009-07-28" version="1">MSSTYTLRQRRQQCAPNKGKAGTSGATAPGKNGLCCFEELPDWQRDNDKILAGYVRETNSMKKCFESLTYFHNETVNIYTHLIPSGTYLVMLMFFTDLFLIPQFPTTTMSDYIMINFFLLGAFLCLMCSTCFHCLKQHSEAQSNIWSKVDYMGIIVLISCSTVSLLYYGFYDYLFHFKMFTIAVVVLGSCCAAFVLNDRFNAKNWRACRAGFFVTFGFSGIVPIVVGLVKFGIAESSKRVQLRFVLLEALFYISGAVIYGFRIPETLLPGKFDFVGHSHQIFHILVVLGSLCHFRALLGSYFFMHTGISSPGLLNLKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEJ1</accession>
    <name>C5DEJ1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0C09702p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0C09702g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552640.1">
        <property type="nucleotide sequence ID" value="XM_002552594.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEJ1"/>
    <dbReference type="GeneID" id="8291520"/>
    <dbReference type="KEGG" id="lth:KLTH0C09702g"/>
    <dbReference type="HOGENOM" id="CLU_1586772_0_0_1"/>
    <dbReference type="InParanoid" id="C5DEJ1"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="168" mass="18298" checksum="92385DB4420F302F" modified="2009-07-28" version="1">MSNEEKPERKSGLLRSSAANVLATLAAVAACLFCSMRLGSILKPIRKAMEENSGATRDLIFKVGQVVSLSALASLPVFVIWTTYRSFKFTRELSAPSEQRPSCAIAGSPTGICIPLKCSRKRYTNSSDLVHCNSLRTFNINSSISEETSILRAPARIKHNGLISYEAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEK3</accession>
    <name>C5DEK3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0C09966p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0C09966g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552652.1">
        <property type="nucleotide sequence ID" value="XM_002552606.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEK3"/>
    <dbReference type="STRING" id="559295.C5DEK3"/>
    <dbReference type="GeneID" id="8291532"/>
    <dbReference type="KEGG" id="lth:KLTH0C09966g"/>
    <dbReference type="eggNOG" id="ENOG502RZS3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_366833_0_0_1"/>
    <dbReference type="InParanoid" id="C5DEK3"/>
    <dbReference type="OMA" id="HISYMRS"/>
    <dbReference type="OrthoDB" id="4064247at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="369"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="760" mass="86803" checksum="CCBE71FCC16E0638" modified="2009-07-28" version="1">MPLLSNRTYHKDTKKCSFVDRTNPYDLQNYDIREDDEITSSDDAGLESSDAGYEDEYEEGSYNVSSGPNTADEGSASASQGEKGKRPRSASPFKRSISSLFKRRGSRDSCVEEKELDIADEDGSCAAIPGIDQVQSDAASSSKFWRSWRLKHERHGSEHVDENNDVESDAFASDKQAICTDATVPLRHGSNIEKLSELQIENTSLEIFETDREGNPDGAFDARKLDESPTSIARGLQLKTRLGIDSAGSQQPEKDSNHGTLGYAEGGDIMSAKVPLLPSPSTPVEEQIFWKKNVPKSEFQHYESIYLTPKDYVALEDQFDRVQVNKNSRTYRNFVNIIELLDGTSENTFQMFSLEEFTTAVLGLVKGEPKEHKGVRPEHHAKLDRTSSCDDHSSLELKKQDRSSSRIRDLEEELSSLRERYDCSVEDAYNCRNELKIVKSELEETRHEVDNTINRMDKIKSDLKEEINRRISTEETLRGELDIAAESAKKELSKSEELRDSLIKSKNEQEALQAETLVLKEQNETSRSRINELVQLVSQLEAEARKAREENSQLQQSFQEKDVLATHAKMANVKLQKDCQRERCKLLDGRRELDLLRRQMELAACHKAEALQFMAQMMMSFRDVLSKDTLQECDSYLGAINSNQLFNRALFNTDGEMTERQLSDQFGQELQRVTEFYRDFAKKRLLDQIFAKHISYMRSNLFLSQQLKGLRQQGQDHEEYISRLLKDCKAQRVLIAKQDNRIETMKKVKQSDFKTGSIQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEK4</accession>
    <name>C5DEK4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DNA mismatch repair protein MSH2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">DNA mismatch repair protein Msh2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0C09988g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the post-replicative DNA mismatch repair system (MMR).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the DNA mismatch repair MutS family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552653.1">
        <property type="nucleotide sequence ID" value="XM_002552607.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEK4"/>
    <dbReference type="FunCoup" id="C5DEK4">
        <property type="interactions" value="1208"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEK4"/>
    <dbReference type="GeneID" id="8291533"/>
    <dbReference type="KEGG" id="lth:KLTH0C09988g"/>
    <dbReference type="eggNOG" id="KOG0219">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002472_10_0_1"/>
    <dbReference type="InParanoid" id="C5DEK4"/>
    <dbReference type="OMA" id="LVRFPQK"/>
    <dbReference type="OrthoDB" id="295033at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032301">
        <property type="term" value="C:MutSalpha complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035822">
        <property type="term" value="P:gene conversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006312">
        <property type="term" value="P:mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051053">
        <property type="term" value="P:negative regulation of DNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03285">
        <property type="entry name" value="ABC_MSH2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.110:FF:000002">
        <property type="entry name" value="DNA mismatch repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1420.10:FF:000015">
        <property type="entry name" value="DNA mismatch repair protein Msh2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000925">
        <property type="entry name" value="DNA mismatch repair protein MSH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1170.10:FF:000009">
        <property type="entry name" value="Mismatch repair ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1420.10:FF:000041">
        <property type="entry name" value="Msh2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1420.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.10">
        <property type="entry name" value="DNA repair protein MutS, domain I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.110">
        <property type="entry name" value="MutS, connector domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011184">
        <property type="entry name" value="DNA_mismatch_repair_Msh2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007695">
        <property type="entry name" value="DNA_mismatch_repair_MutS-lik_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007861">
        <property type="entry name" value="DNA_mismatch_repair_MutS_clamp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007696">
        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016151">
        <property type="entry name" value="DNA_mismatch_repair_MutS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036187">
        <property type="entry name" value="DNA_mismatch_repair_MutS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007860">
        <property type="entry name" value="DNA_mmatch_repair_MutS_con_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032642">
        <property type="entry name" value="Msh2_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036678">
        <property type="entry name" value="MutS_con_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361:SF35">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MSH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01624">
        <property type="entry name" value="MutS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05188">
        <property type="entry name" value="MutS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05192">
        <property type="entry name" value="MutS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05190">
        <property type="entry name" value="MutS_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005813">
        <property type="entry name" value="MSH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00533">
        <property type="entry name" value="MUTSd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48334">
        <property type="entry name" value="DNA repair protein MutS, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00486">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 11" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8 11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair proteins mutS family" evidence="13">
        <location>
            <begin position="756"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="483"/>
            <end position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00073545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS00486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR22215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="955" mass="106259" checksum="D41354C454B713C1" modified="2009-07-28" version="1">MSSSRPELKFFDISEERGFYKRFSTLPEKPPKTIRIVDKGEYYTVVGNDALFVADNVYHTNSVLKDCRIDPSTAKSIQEPLKYVTISSQVLSGLLKLCLLEQGMKVEIYDKSWKLLRNGSPGNIEQVDDLMNISLDSSIVLTSIKIQPNAQEGNCVLGVSFIDSSSYKIGMLDIVDNEVYSNLESFLIQMGVKECLLPDLRSNETVANELKKMVGVLDRCGCVSTFVKNSDFSHRDVDQDLVKLVGDELSMSLMKFSNLALGSCNALLSYLQLLNDESQLGRYELVQHSLTEFMKLDASALKALNIFPTGPSGASGVSVSHGGGSGGKVSSIFQLLNKCKTNAGVRLLNQWLKQPLTDLQAIQNRHDLVEFLIDQLELRQVLQSDYLPLVPDVRRLTKKLNKNGGLEDVLKIYQFARKIPEVSQVLEDYLGNMDGGDSIKPLVTETWLQPLNAHITPLDKFQEMVETTVDLEAYEDTNEFMIKVDFNEDLAKIRGELDALKDNIKSIHLDAAEDLGFDPEKKLKLENHHMHGWCMRLTRNDGKALRQHRKYLELTTVKAGIYFSTRELKQIAEQTAILQKEYDRLQYDLVKEIVQITLTYTPVLEKLSIVLANLDVLSAFAHVSSYAPIPYVRPQMHGLGTQRKTELIGSRHPVLETLDDVTFISNDVRLESGKSEFLIITGPNMGGKSTYIRQVGVISLMAQIGCFVPCDTAEIAVVDAVLCRVGAGDSQLKGVSTFMVEMLETASILKNATENSLIIVDELGRGTSTYDGFGLAWSISEHIARNVRCFALFATHFHELTSLADQVPTVQNMHVIAHIEDARASTHSADDITLLYKVEPGISDQSFGIHVAEVVQFPAKLVSMAKRKAAELEDLKHDSQALKRQKCSAGEIAEGTRVLKDVLKAWAAAVRAEGLHEKLSDDESQARVLELLNQVSAASAQTDNKFMKEIAELLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEK6</accession>
    <name>C5DEK6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0C10032p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0C10032g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552655.1">
        <property type="nucleotide sequence ID" value="XM_002552609.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEK6"/>
    <dbReference type="FunCoup" id="C5DEK6">
        <property type="interactions" value="493"/>
    </dbReference>
    <dbReference type="GeneID" id="8291535"/>
    <dbReference type="KEGG" id="lth:KLTH0C10032g"/>
    <dbReference type="eggNOG" id="ENOG502QZJZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008153_0_0_1"/>
    <dbReference type="InParanoid" id="C5DEK6"/>
    <dbReference type="OMA" id="DNMARFE"/>
    <dbReference type="OrthoDB" id="2123952at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050987">
        <property type="entry name" value="AtrR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46910:SF3">
        <property type="entry name" value="HALOTOLERANCE PROTEIN 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46910">
        <property type="entry name" value="TRANSCRIPTION FACTOR PDR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="610"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="661"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="8">
        <location>
            <begin position="21"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="72"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="787"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="72"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR22217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="866" mass="97774" checksum="50AD67B13D835CBA" modified="2009-07-28" version="1">MAPESPSELSAFKFRKRVPKACDHCRKRKIKCGAVNPITGTCDNCTKFNTVCTFKHHDEIGRHRKYAELKKLQSHEPKVRKVSESSSPGASATDRKLEQLEYQIARLHELTARQQYQAGVCSRHSPSLGVDTIPDMSPAPKQKRYFTSLLTKRRIAWLRNQAKAFSANQETPGDAGSPMLSFYPLTDAFMVASKWYVAQVKKIIDFSNPFVPFNSSQLYPLPPQEHANIAVKAIQENLSPGSFNVVRGPELQALFDKHYANQKLTYSEILLLDIILTVSNSYPLTDDPRRLKEGESEHSTVESHMLLNAINQYHKVALLSEGVRSVQALLILYQYVSCHISGEAAYNIFNTTQRFAQDIGLNRKESYKNLSLEEASLRLKMWYCCVLADSQLSLAFCRAPLIDLEDTSVLTEEFYMELVKERHLGNSDRTSIQTLDEAIQSLLGSSDTFQLGTAYYGMTLTRTFHKAYRRLLAANSMTNVTFDQVIEGVLDIKSDLIRWEKSLPPILALGEYEENLTRLRNASGDPLSAKHFEILSTTILQLHYEHLYLNLIVSQIACSFVFDNEDILPKSRYNVAALRRTFVNDACDSAVQMMKLWPSIKVVPHMVHRLFYTFSTGAFVLLLTVIAFPNEDRVLEYISTMCTTVEYLLEMGEYRVFKDNIKWNVTMFVFCFLLTLSINYFNACNPQAKDANFSSAPFRNSLEALMNRCKMNKDVAVNQLREHLNIFAPGFDENSEMHENPDISSGTCDASKGLPRTLYLFSEVDEEDLRCLMSSLPVQIPCSNRFGPETEAKGSAMQASPSKGSQHGFRSGPSADNDFAAAFSLGLPSSDEPQTLAGGPPSDQVDFENMLDDMLFDRDFTFPTLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEL8</accession>
    <name>C5DEL8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0C10296p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0C10296g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS28 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552667.1">
        <property type="nucleotide sequence ID" value="XM_002552621.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEL8"/>
    <dbReference type="SMR" id="C5DEL8"/>
    <dbReference type="FunCoup" id="C5DEL8">
        <property type="interactions" value="884"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEL8"/>
    <dbReference type="GeneID" id="8291547"/>
    <dbReference type="KEGG" id="lth:KLTH0C10296g"/>
    <dbReference type="eggNOG" id="KOG3502">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_178987_1_0_1"/>
    <dbReference type="InParanoid" id="C5DEL8"/>
    <dbReference type="OMA" id="NTGMHGE"/>
    <dbReference type="OrthoDB" id="10258930at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04457">
        <property type="entry name" value="S1_S28E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000025">
        <property type="entry name" value="40S ribosomal protein S28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00292">
        <property type="entry name" value="Ribosomal_eS28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000289">
        <property type="entry name" value="Ribosomal_eS28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028626">
        <property type="entry name" value="Ribosomal_eS28_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10769">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10769:SF3">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN ES28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01200">
        <property type="entry name" value="Ribosomal_S28e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00961">
        <property type="entry name" value="RIBOSOMAL_S28E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR22229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="67" mass="7551" checksum="ABF4032F8F18A641" modified="2009-07-28" version="1">MDSKTPVTLAKVIKVLGRTGSRGGVTQVRVEFLDDTSRTIVRNVKGPVRENDILVLMESEREARRLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEN1</accession>
    <name>C5DEN1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ordered locus">KLTH0C10604g</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the HRD1 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552680.1">
        <property type="nucleotide sequence ID" value="XM_002552634.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEN1"/>
    <dbReference type="FunCoup" id="C5DEN1">
        <property type="interactions" value="266"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEN1"/>
    <dbReference type="GeneID" id="8291561"/>
    <dbReference type="KEGG" id="lth:KLTH0C10604g"/>
    <dbReference type="eggNOG" id="KOG0802">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026577_0_0_1"/>
    <dbReference type="InParanoid" id="C5DEN1"/>
    <dbReference type="OMA" id="ANLTMFT"/>
    <dbReference type="OrthoDB" id="7759664at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16479">
        <property type="entry name" value="RING-H2_synoviolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050731">
        <property type="entry name" value="HRD1_E3_ubiq-ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057992">
        <property type="entry name" value="TPR_SYVN1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058051">
        <property type="entry name" value="Znf_RING_synoviolin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22763:SF184">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SYNOVIOLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22763">
        <property type="entry name" value="RING ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25563">
        <property type="entry name" value="TPR_SYVN1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="14 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="46"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="146"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="18">
        <location>
            <begin position="314"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="379"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAR22242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="515" mass="58487" checksum="5187F80FD3480756" modified="2009-07-28" version="1">MALRVQRRQFVGYTVFTYTAAAWSVLDCLQPSLSFLDACLKLCEGLNLVVLCNFAIVNSILLWKGLTRLLFGELRLLEYEHIFERLSFTIVNCFFMSSAFPEYQFLTVMILFALLTFIRVFHWVLKDRLEYVFQHTDERTNIARLLCSRFFLNVIIFAVVDYQMVRFCLRNSLTNKHDPSPSAPVYILFCVDFSMLLVDVAEVAMKSVINLIELIQCKRAFERDGDDFVGLDGKFMYEKLVQLVCQVLKLGLRVASLAPFSMPLMIAKDIIWDGIALFHTGKSVWRTWKSNRQIDEKLPDVTEAQLNASEDKMCIVCMEDMLPPSEATSAKHKPKKLPCNHCLHLGCLKSWMERSQTCPICRVSVFDSKGNVALPNQNAAQQPISEDASVAQQPLSAANESNSTSLASTHTGSLGAVSSSNPNFPTWYTFPVTLSDNDGAVDFKVRDINGVEINAKLIIKKRPGFDVPKEQDQESQRVVINDPPATQYQDIERLKRRISELENKVDELTKKARTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>C5DET4</accession>
    <name>C5DET4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0D09592p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0D09592g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553127.1">
        <property type="nucleotide sequence ID" value="XM_002553081.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DET4"/>
    <dbReference type="FunCoup" id="C5DET4">
        <property type="interactions" value="210"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DET4"/>
    <dbReference type="GeneID" id="8295365"/>
    <dbReference type="KEGG" id="lth:KLTH0D09592g"/>
    <dbReference type="eggNOG" id="KOG1343">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024583_0_0_1"/>
    <dbReference type="InParanoid" id="C5DET4"/>
    <dbReference type="OMA" id="GTQDICW"/>
    <dbReference type="OrthoDB" id="5232919at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004407">
        <property type="term" value="F:histone deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010557">
        <property type="term" value="P:positive regulation of macromolecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd09998">
        <property type="entry name" value="HDAC_Hos3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.800.20:FF:000011">
        <property type="entry name" value="Histone deacetylase HOS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053244">
        <property type="entry name" value="HDAC_HD_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47558">
        <property type="entry name" value="HISTONE DEACETYLASE HOS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47558:SF1">
        <property type="entry name" value="HISTONE DEACETYLASE HOS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="2">
        <location>
            <begin position="93"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="606"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="617"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="650"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="688"/>
            <end position="705"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR22689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="705" mass="78456" checksum="953209615D751681" modified="2009-07-28" version="1">MSKEVDPLLKFEHQFQSFVRNNPNVASAAHSASQIPESAKAVVVLSPLSLQHQFPRYWVSKSYKKTIVERPERLLACSMGIGAAITMYPALFTLKSSHKRKTSLLSDHVLKVHGSDWPQELLKLCKNSQKALDQCQVEVPESWNVGDIYLSRQTISALEGSIGALETGVDSIFHGPSVEQTSNRAFVALRPPGHHSHASTPSGFCLLNNAHIAIEYAADTYGVTHAVVLDFDLHHGDGTQDICWKRAGFKPDGCDDGGSEYNEFGKKFAEFPKVGYFSLHDINSFPTELGYATKENVRNAATCIMDAHDVNIWNVHLQSYDSDEAFTKLYQTKYRTLFAKADEYFRTAKQNMRALGKPFKGLVVISAGFDASEYEQPSMQRHAVNVPTFFYNLFTKDALKLAQMHSHGKLLSVLEGGYSDGAISSGVFAHLIGLQNQNWINEWGSAQVVKEIVRGCKPQWKPYKTKRARDVIRIWAEEVIKLGRAMIPEFEQVLFEPELVEPKAETSKRITRSRRAPAAASIAVVKTPPTLPRSETPTPNDKRYIQKLETADVETVPFVEQDLPSDNEDEDDEDYVYDEELNKTFNRTVEDITIDDISRHLETLEIEPEALDDAPPRTASSSGTRASSRNADSEAAAAAAAGAPQERRPLSSSSGGYKIPSGSTTRHLRNTRASRLMNFDDSDISMISHVSPTKGRSTRSGQTKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEX3</accession>
    <name>C5DEX3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0D10494p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0D10494g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553166.1">
        <property type="nucleotide sequence ID" value="XM_002553120.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEX3"/>
    <dbReference type="FunCoup" id="C5DEX3">
        <property type="interactions" value="28"/>
    </dbReference>
    <dbReference type="GeneID" id="8295405"/>
    <dbReference type="KEGG" id="lth:KLTH0D10494g"/>
    <dbReference type="eggNOG" id="ENOG502RXWC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035821_1_1_1"/>
    <dbReference type="InParanoid" id="C5DEX3"/>
    <dbReference type="OMA" id="RIYVNCS"/>
    <dbReference type="OrthoDB" id="2556122at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044695">
        <property type="term" value="C:Dsc E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045226">
        <property type="entry name" value="Dsc3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025390">
        <property type="entry name" value="Dsc3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019413">
        <property type="entry name" value="Dsc3_ub-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28049:SF1">
        <property type="entry name" value="DSC E3 UBIQUITIN LIGASE COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28049">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN YOR223W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13373">
        <property type="entry name" value="Dsc3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10302">
        <property type="entry name" value="Dsc3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="201"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="231"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="DSC E3 ubiquitin ligase complex subunit 3 ubiquitin-like" evidence="2">
        <location>
            <begin position="15"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="DSC E3 ubiquitin ligase complex subunit 3 C-terminal" evidence="3">
        <location>
            <begin position="126"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR22728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="252" mass="28542" checksum="5A5ED2AF671DDECE" modified="2009-07-28" version="1">MSGSELDGSPSQDRVVVVRFSDEQMEDLPLNVNNVSLSDVNTSWLRRMCRQLRGRSLSNRKLRFIRSGRFLNASTDLQLQRYFESTNDKFYVHCIVGQELTPQELANEDSVDETQQNTEGTTTQAIGFDRLRAVGFSDEEIELLRQRFRSTYGDLEELSRDNGEGQDIRQLEEHWMETGANDENDQLGAVGIANYKHNKDLLIGLVVGCVLGVFSFLLMKQEGLFNKRQKLAMVGGLLINMGFGAMLGFSRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DF11</accession>
    <name>C5DF11_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0D11374p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0D11374g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily. AB hydrolase 4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553204.1">
        <property type="nucleotide sequence ID" value="XM_002553158.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DF11"/>
    <dbReference type="FunCoup" id="C5DF11">
        <property type="interactions" value="274"/>
    </dbReference>
    <dbReference type="ESTHER" id="lactc-c5df11">
        <property type="family name" value="abh_upf0017"/>
    </dbReference>
    <dbReference type="GeneID" id="8295444"/>
    <dbReference type="KEGG" id="lth:KLTH0D11374g"/>
    <dbReference type="eggNOG" id="KOG1838">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032487_1_0_1"/>
    <dbReference type="InParanoid" id="C5DF11"/>
    <dbReference type="OMA" id="MMTTSWG"/>
    <dbReference type="OrthoDB" id="5954035at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008126">
        <property type="term" value="F:acetylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051792">
        <property type="term" value="P:medium-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051793">
        <property type="term" value="P:medium-chain fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000952">
        <property type="entry name" value="AB_hydrolase_4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050960">
        <property type="entry name" value="AB_hydrolase_4_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012020">
        <property type="entry name" value="ABHD4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10794">
        <property type="entry name" value="ABHYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10794:SF63">
        <property type="entry name" value="ALPHA_BETA HYDROLASE 1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005211">
        <property type="entry name" value="Ab_hydro_YheT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01133">
        <property type="entry name" value="UPF0017"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0719">Serine esterase</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="5">
        <location>
            <begin position="141"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="4">
        <location>
            <position position="222"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="4">
        <location>
            <position position="354"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="4">
        <location>
            <position position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR005211-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR22766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="443" mass="50285" checksum="14E39E7F57F54D38" modified="2009-07-28" version="1">MESILHHLPIYSHVRQTRPFEPLKFVGEQSGTSTDLPTLVDKCSPEFSNGAMALFHPLYFNGHLQTLYAAVRTFDKENMVYYKRLVVPCRDGGEATADFRVPPFEKASDYTPPNQTKPLMPRYSFFTPEEERNLGSNDEKPLLIVLHGLTGGSHEAYVRSLVDRITTNYGFEACVLNSRGCSESCITTPQLYNGVWTNDIRDFVVKLRALFPKRKFYLVGFSLGASIATNYLGQEGSNSQIECAAILANPWDLCNASYYMYRTFLGRNLYGNHFSKSLVKMLKDNLSVLKQDPYMAKLYAEKIDSVKTVEEFDNWFTSRMFGFNCSFEYYRYGTSSNRIPLIRTPMLAISASDDPIVGSESLPIREIESNPYTLLIESSKGGHVAWFDAHGERWYEEPLCRFFSGFHSDVALEGLKPDLASVTLPHANKFKGDRLVDATVRDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>C5DF17</accession>
    <name>C5DF17_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0D11506p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0D11506g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553210.1">
        <property type="nucleotide sequence ID" value="XM_002553164.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DF17"/>
    <dbReference type="FunCoup" id="C5DF17">
        <property type="interactions" value="72"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DF17"/>
    <dbReference type="GeneID" id="8295450"/>
    <dbReference type="KEGG" id="lth:KLTH0D11506g"/>
    <dbReference type="eggNOG" id="ENOG502S1HE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040321_2_1_1"/>
    <dbReference type="InParanoid" id="C5DF17"/>
    <dbReference type="OMA" id="LNCFQYF"/>
    <dbReference type="OrthoDB" id="431202at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022127">
        <property type="entry name" value="STIMATE/YPL162C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31735">
        <property type="entry name" value="VACUOLAR MEMBRANE PROTEIN YPL162C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31735:SF1">
        <property type="entry name" value="VACUOLAR MEMBRANE PROTEIN YPL162C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12400">
        <property type="entry name" value="STIMATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="163"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="204"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="269" mass="30716" checksum="9D8E6F9AE4F6F9B5" modified="2009-07-28" version="1">MNSGTGDDSCQLLGPISLLIQTLMGVAAISGLLLKRRYEVPKRRWRVWFFDISKQVFGSLIIHFLNLGISILKQRKAHFLMVLDDDPGGDQCDWYFLNLLMDTTVGTLVLWFVLGLLEEGLRRLGVQNIESGNYYPSRDSSRETEEGPPIRNEPLFSAFGKQLLIFVCGLSIMKFCVFLILTYFEVVATWFAELVLGWSDNWPNFQVFLVMFVFPILLNFFQCFCIDNIIKLHPRNLSTSELENFEEGPILYSPGLQNASHTKAMYGST</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DF93</accession>
    <name>C5DF93_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0D13244p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0D13244g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553286.1">
        <property type="nucleotide sequence ID" value="XM_002553240.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DF93"/>
    <dbReference type="FunCoup" id="C5DF93">
        <property type="interactions" value="55"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DF93"/>
    <dbReference type="GeneID" id="8295528"/>
    <dbReference type="KEGG" id="lth:KLTH0D13244g"/>
    <dbReference type="eggNOG" id="KOG0037">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_863808_0_0_1"/>
    <dbReference type="InParanoid" id="C5DF93"/>
    <dbReference type="OrthoDB" id="186625at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16180">
        <property type="entry name" value="EFh_PEF_Group_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051426">
        <property type="entry name" value="Peflin/Sorcin_CaBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46212:SF3">
        <property type="entry name" value="GH27120P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46212">
        <property type="entry name" value="PEFLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="140"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="101"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="102"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR22848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="311" mass="34891" checksum="0229D9A9251E4010" modified="2009-07-28" version="1">MRAGQQREQREQREQEERRRRQKRQEQRLPPPRNGRVGSAPPAVPQHYMGPAPGAVPVSRYAPIPAAPVVRVPRSRPAPAPAAASMPAAMPVAAAAPLAYSPSPPQPAPAPVPMSAPTTARNSLESDTQQSFKDTQIATQLFQNHDVKQRGRLTAAELQNLLQNDDNSHFCISSVDALINLFGASRFGTVSLTEFISLYRRVKKWRKVYVDNDINGSFTISASEFHNSLQELGYLVPFDVSENLFDQYAEFIDPGKNGKELKFDRFVETLVWLMRLTKVFRKFDTNQEGVATVQYKDFIDATLYLGRFLPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFD9</accession>
    <name>C5DFD9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0D14322p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0D14322g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the nuclear import and ribosome assembly adapter family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553332.1">
        <property type="nucleotide sequence ID" value="XM_002553286.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFD9"/>
    <dbReference type="FunCoup" id="C5DFD9">
        <property type="interactions" value="267"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFD9"/>
    <dbReference type="GeneID" id="8295576"/>
    <dbReference type="KEGG" id="lth:KLTH0D14322g"/>
    <dbReference type="eggNOG" id="ENOG502RYAI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_446315_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFD9"/>
    <dbReference type="OMA" id="ADMDMVT"/>
    <dbReference type="OrthoDB" id="288703at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13394">
        <property type="entry name" value="Syo1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052616">
        <property type="entry name" value="SYO1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057990">
        <property type="entry name" value="TPR_SYO1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13347">
        <property type="entry name" value="HEAT REPEAT-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13347:SF1">
        <property type="entry name" value="HEAT REPEAT-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25567">
        <property type="entry name" value="TPR_SYO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SYO1-like TPR repeats" evidence="3">
        <location>
            <begin position="383"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00049983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR22894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="617" mass="69337" checksum="B3D991DC8561CEF8" modified="2009-07-28" version="1">MGKSKRRSKASRSHLNPLGGKNKSTNRDEAMSVKKIQPLLKQLNSAAPNDRAMALGSVTVLCEDPYMRKLFLKEKLLHLIMSKLLSDDNMEIVVESYGLLRNLALEEGYDVCVFLWRSDIWTCIKSGLEKLLKSLESLKANIKSSAESTRLLFDFGDNLVSVVVALTSGSDSILEDFLKSDKLACMFSVLGSVLNYALVEKDLKVSLRIPVSFFNTILDFIYDLSSESLGFIDAVAQDAFLAEFVKSLPTMQLTNANGLTTVLIQGIYLQFLDMDVSSEQVNEILGKTCSAIETIDLAEMKKSLSTKDFDDSVASLPDKEVSGKIKELNKRRAQASVSLQSIEVTLDIITASLEIVAAQVERANAQLDDSMLQTLTVSLPMVFQSLFADFRSRILIAWNNMLWLYLTLQINFFELPNNMWQHLWDSLVSETPEEQADFSMRLGKLGVMWALLKTAQMQEDNVAFLSKFNCANSAFVEAIETQYNLVQELDPSEDQELKQRCIGILACLASLPGHVELNRQIGQFLIQKLADEHTPAATMIDISDALFDIYSDANFDYDEPVFVGDGFINVLQEKVLPSMRKCFKFVDKNKDPELKARSQSCFGTMERFIHYKVDERK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFL6</accession>
    <name>C5DFL6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-(apurinic or apyrimidinic site) lyase</fullName>
            <ecNumber evidence="2">4.2.99.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0D16126g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-phospho-2'-deoxyribonucleoside-DNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66592"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:16897"/>
            <dbReference type="Rhea" id="RHEA-COMP:17067"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:157695"/>
            <dbReference type="ChEBI" id="CHEBI:167181"/>
            <dbReference type="EC" id="4.2.99.18"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the type-1 OGG1 family.</text>
    </comment>
    <dbReference type="EC" id="4.2.99.18" evidence="2"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553409.1">
        <property type="nucleotide sequence ID" value="XM_002553363.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFL6"/>
    <dbReference type="FunCoup" id="C5DFL6">
        <property type="interactions" value="644"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFL6"/>
    <dbReference type="GeneID" id="8295656"/>
    <dbReference type="KEGG" id="lth:KLTH0D16126g"/>
    <dbReference type="eggNOG" id="KOG2875">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027543_3_1_1"/>
    <dbReference type="InParanoid" id="C5DFL6"/>
    <dbReference type="OMA" id="ITKMCHS"/>
    <dbReference type="OrthoDB" id="238681at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034039">
        <property type="term" value="F:8-oxo-7,8-dihydroguanine DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006285">
        <property type="term" value="P:base-excision repair, AP site formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="entry name" value="Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012904">
        <property type="entry name" value="OGG_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052054">
        <property type="entry name" value="Oxidative_DNA_repair_enzyme"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10242">
        <property type="entry name" value="8-OXOGUANINE DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10242:SF2">
        <property type="entry name" value="N-GLYCOSYLASE_DNA LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07934">
        <property type="entry name" value="OGG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55945">
        <property type="entry name" value="TATA-box binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HhH-GPD" evidence="11">
        <location>
            <begin position="126"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="364"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR22971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="386" mass="45395" checksum="5228E5B956021554" modified="2009-07-28" version="1">MLKFQKLLFKRGELYLDKVLQCGQAFRWIFHEGLGQYSTTMRIDDRFRIVVLRQLEDNYIEYASLGAEECSSLGSFLKRYFRLEVPLSELYENQWLPRDSRFEKKRPHGIRILSQDPWETLLSYICSSNNNISRITKMCHALCIEFGNPVGQYDKVDYYSFPTSKELVERASEEKLRALGFGYRAKFLMKTADKMLKERLDMSDTQYLESWKDHLEYEQVRERVMGFDGVGPKVADCVCLSGLEMDEVVPVDVHIARIAQRDYRFVPRRQDIEELQIRYKNLPITRKKVNYELDLIRAMFKEKWGDFAGWAQGIVFAQEVGKTIGATSEGTTSRRKLEFEVKVELPSQGNSVKRELDEMVEEEIEYSDTGRPKRKTTKRVEYRFKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFM3</accession>
    <name>C5DFM3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0D16280p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0D16280g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553416.1">
        <property type="nucleotide sequence ID" value="XM_002553370.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFM3"/>
    <dbReference type="FunCoup" id="C5DFM3">
        <property type="interactions" value="18"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFM3"/>
    <dbReference type="GeneID" id="8295663"/>
    <dbReference type="KEGG" id="lth:KLTH0D16280g"/>
    <dbReference type="eggNOG" id="ENOG502R7IE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042615_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFM3"/>
    <dbReference type="OMA" id="WSGLEWC"/>
    <dbReference type="OrthoDB" id="4062523at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051285">
        <property type="term" value="C:cell cortex of cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009571">
        <property type="entry name" value="SUR7/Rim9-like_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052413">
        <property type="entry name" value="SUR7_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28019">
        <property type="entry name" value="CELL MEMBRANE PROTEIN YLR413W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28019:SF6">
        <property type="entry name" value="PROTEIN ECM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06687">
        <property type="entry name" value="SUR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="26"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="201"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="234"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="285"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="343"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="344"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR22978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="402" mass="44641" checksum="FE77C3CBE04190C4" modified="2009-07-28" version="1">MLVRRVKKVASKPFENLGALDRLIQYLRLGSSLIVAVLVMVLTLGALVSPSKIFIGKLHTCLGDIAQGLFDALQQSVEAKTANYVNNGVGLTTSEILILTEYTTSQVQSSPLFVVSSMYGWCRVDNGTSSASNATFRNHTQCHTVGSTYVFNYRELMKEIGLNAILEYTYGTLDGNSYEVSKPYVEYMDKLQTTKIRMVNVLYMVGCSHMVMVFCSLWYYSIKGRSLSILKERVLVHLISLLSLVFCICAMVSAVTLATISIQLRSRIKSELHYMGISYHLGRTWFAALWLLAIFSCISCMVWSGLVWCIAEKSADSIESYELGILATTNQTQDNDEATFVDQKPQEKQTEHLKPPPQASSSQARLLDDGDGEVLRSVTTSASDYSETSHQRVVVPLSAFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFN9</accession>
    <name>C5DFN9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="16">KLTH0D16654p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">KLTH0D16654g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553432.1">
        <property type="nucleotide sequence ID" value="XM_002553386.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DFN9">
        <property type="interactions" value="120"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFN9"/>
    <dbReference type="GeneID" id="8295680"/>
    <dbReference type="KEGG" id="lth:KLTH0D16654g"/>
    <dbReference type="eggNOG" id="KOG1012">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001661_1_1_1"/>
    <dbReference type="InParanoid" id="C5DFN9"/>
    <dbReference type="OMA" id="DHFYGDW"/>
    <dbReference type="OrthoDB" id="1029639at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061817">
        <property type="term" value="P:endoplasmic reticulum-plasma membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04044">
        <property type="entry name" value="C2A_Tricalbin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04052">
        <property type="entry name" value="C2B_Tricalbin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04045">
        <property type="entry name" value="C2C_Tricalbin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04040">
        <property type="entry name" value="C2D_Tricalbin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21678">
        <property type="entry name" value="SMP_TCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037761">
        <property type="entry name" value="C2A_Tricalbin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037765">
        <property type="entry name" value="C2B_Tricalbin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037762">
        <property type="entry name" value="C2C_Tricalbin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037756">
        <property type="entry name" value="C2D_Tricalbin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031468">
        <property type="entry name" value="SMP_LBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056910">
        <property type="entry name" value="TCB1-3_C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017147">
        <property type="entry name" value="Tricalbin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052455">
        <property type="entry name" value="Tricalbin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46980">
        <property type="entry name" value="TRICALBIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46980:SF1">
        <property type="entry name" value="TRICALBIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24920">
        <property type="entry name" value="C2_TCB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25669">
        <property type="entry name" value="SMP_MUG190-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037232">
        <property type="entry name" value="Tricalbin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51847">
        <property type="entry name" value="SMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="9" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="207"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="SMP-LTD" evidence="15">
        <location>
            <begin position="272"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="14">
        <location>
            <begin position="470"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="14">
        <location>
            <begin position="634"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="14">
        <location>
            <begin position="757"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="14">
        <location>
            <begin position="1104"/>
            <end position="1220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="68"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="621"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1309"/>
            <end position="1355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1373"/>
            <end position="1400"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="931"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="69"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="621"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="1322"/>
            <end position="1331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAR22994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1540" mass="168732" checksum="DAA37FB95F5CE104" modified="2009-07-28" version="1">MLSDKYNSDGSSRILTVSQLCKNRGLAARLGSVNMPNNATLPVDSNLESKEAKVGGKLAEKAYADMPSVNKSVTGSGSVKQRRSTSSYPGSNKDILVASNAPGVMKLPPPNANGTVGPAPLEITDPKEAKHLTPREKNLHETSIDKSAYEQQQRTIETLSENLAVDKPEMLYPWGSTGAFYGSKNGPASADSNVVKAYITERFYNDIYYNMAGIIGTCFFSWLLAHWGFSWWSLGFVFFCTASVYRSEFRRFNRNIRDDLTRITVEETLSDRTESTLWLNSFLSKFWVIYMPVLSQQVKDIANPQLAGVAPGYGIDAISLDEFTLGTKSPTIDGIKSYTKKGKDTVEMDWVFSFTPNDVSDMTRKEAKEKINPKIALGVTVGKGFVSKSLPVLVEDINCKGRLRITIKFGPAFPNIKIVQLSLLEPPFIDFALKPVGGDTLGLDIMSFLPGLKTFVKTMINSNVGPMLYNPHHLDIDVEEIMAAQSQDAIGVVAVTIHSADDLKGSDFIGNTVDPYVTLTAEKGNIGETTIRTSVKSDVKSPRWNETKYVLVNTLEQKLYFTCYDFNDIRKDTVIGKVELELNGLFQNPTLENKTSKITSGGRSRGTLNYDVHWLPVVNKEEQEAREANEGAPERAEEEDNAETNQSASDVGILKATLHKITNLKHATAVQGALSPSAELFVDGKSVMHFRTLRRINEPSWEQSIEVLVPSKKNSTLLLKVYDQGIAGREELCKYSTSMEDVLSMADAGQSVLQASPQGRIYMSAAWKPVVMTGSFAPANSVRDPIGVARLHIQDALVDENLSGVGDIDPYITVSLNKRITHRTHYFSDNREPNFNAVVYIPVTSEKQTIVVDVIDYQKVGKDRPIGSYSIPVSKLLEKKTDSGVIGQSDEPPSQCRLLGKNMKLTKSYINIAMSLLPVTQVYSPDELGKVEQLEAKIQERKEKFAKDQEELKKKMEKDPDLYEVVEIDVDDEDEKALKKKEKRSVTELLKENSGVLSFQILRGKLSKHSAHLQVLFDDLACPASTSMKSRKGEAIPDSGSCFIRDLKHSRITFRLTKKFEPKELSDVLSEASYPTSELLKKGLEKPTVVEFEGSKIEVHFLYTPASVELPPSESIEDTGIMKLKVISADGLPSHDRNNKSDPYVAINVDGSEVQKTEVIKKTLSPVWNEELEIPVPSRSRSKVVVEVYDWDRTGSNDLLCSTVLDTKILVPEKTKDMDLKLEKQGTIKLAATFIPEYLFPTLDPVKGGLASRPLKAIGGVANLGVGVAGAGIGAAQNVAGAGLGAAQNVAGAGFGGIQKGGRFLKSFGTKKSKKDAKDSKMDEEEGEEGAGSDQQARENENRSLAPKLSADYDVTKPNTSYAKVESYHPAPAVASKNGDGSSVVGAPGPIHQRSASTASSFARTLAPNGTYKGKVTLVGAENMGKALQVRFSLAQGGKIKHMYRTENKKSNSEGWVDFGESCEFKASPEANLVFGGVTVHRFGKDTELGVAQINLGDPQIQQEGQISVRLGRGHLILKIDYGNDEDVPPVPSIPSEYKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFW0</accession>
    <name>C5DFW0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0D00374p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0D00374g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552740.1">
        <property type="nucleotide sequence ID" value="XM_002552694.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFW0"/>
    <dbReference type="FunCoup" id="C5DFW0">
        <property type="interactions" value="136"/>
    </dbReference>
    <dbReference type="GeneID" id="8294959"/>
    <dbReference type="KEGG" id="lth:KLTH0D00374g"/>
    <dbReference type="HOGENOM" id="CLU_708003_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFW0"/>
    <dbReference type="OMA" id="KRISNIX"/>
    <dbReference type="OrthoDB" id="4037327at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001142">
        <property type="entry name" value="DUP/COS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00674">
        <property type="entry name" value="DUP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="215"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="251"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="373" mass="43114" checksum="988866C71EB484D8" modified="2009-07-28" version="1">MNKACVPDKEAQNDFLPKFFYKSSLRWALDQLMRMPLFYLLLSSIVIDIVWFSIGNKLHSFWIHVPVFSSLLLQIKVTKAISNSIQRLCEIVIEEGPGMQEEQWDIAAARMNSHFYEQGLWPNSCCLYSGRQCHAAFRHLVMTPYNKKEEQIQASYSLDQQLPLEEQAAKVYIESVSRWWNTYTANLESSAQFHIENTLPQQEYRSKASWCFFKFIEVVMGQSSFGGMCFYSILLSLFFTSFTSFKNYDSLLGVWIGNAAGFSICGPLLFFVLRGADLDAPHLMAFWASVVAIEPHGDINKWGLVASHTNHYWNALNEESPRNFFFDSMDCRKAFKNRFSTSLLRNKGNSHIYSDLRKYIEEALEASKEINEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DG44</accession>
    <name>C5DG44_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0D02288p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0D02288g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC29A/ENT transporter (TC 2.A.57) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552824.1">
        <property type="nucleotide sequence ID" value="XM_002552778.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DG44"/>
    <dbReference type="FunCoup" id="C5DG44">
        <property type="interactions" value="292"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DG44"/>
    <dbReference type="GeneID" id="8295044"/>
    <dbReference type="KEGG" id="lth:KLTH0D02288g"/>
    <dbReference type="eggNOG" id="KOG1479">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021611_3_0_1"/>
    <dbReference type="InParanoid" id="C5DG44"/>
    <dbReference type="OMA" id="GSPWTTK"/>
    <dbReference type="OrthoDB" id="46396at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034257">
        <property type="term" value="F:nicotinamide riboside transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015205">
        <property type="term" value="F:nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002259">
        <property type="entry name" value="Eqnu_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10332">
        <property type="entry name" value="EQUILIBRATIVE NUCLEOSIDE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10332:SF88">
        <property type="entry name" value="EQUILIBRATIVE NUCLEOSIDE TRANSPORTER 1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01733">
        <property type="entry name" value="Nucleoside_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016379">
        <property type="entry name" value="ENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="73"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="133"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="172"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="200"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="259"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="291"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="328"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="363"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="406"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR22386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="430" mass="48021" checksum="D9753F9949877E60" modified="2009-07-28" version="1">MDNFREGSPSTELHRHIGEELELENATLWVKLQDLNYTTFLLIGIALLWPWNSFLSASLYFQHDVFHDKTVFAKIYISTMMSISTISSVIFNYWLSKRQHSYSRRIVRGLIWEIMVFGLLSMFVFVHKSLPDWLNFTFLMSTVLVSSLGTAMTQNGAMALANVFGPQFSQGVMVGQAVAGVLPSLVLFLVSYIGDPRDQSVGGIFAYFGSTVLVSVACIVLYRVSSIGSADKHDFLNRELDPEATVSVPFSLLFDKLRYLVLSIFTTFVVTLLFPVFAANTFVSGLPMRNAQYIPFIFTVWNLGDLYGRAISDYSFFQSPSFTPFKTFLYSIARIGLVPLFFCFNLNSSARPSSSFSAIVSDLLYILLQFAFGVTNGNVLSVSFMKVSSQLNSDKQRKAAGGFTNIFLSAGLAFGSLLSYLFTYIVLSKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5DG57</accession>
    <name>C5DG57_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0D02574p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0D02574g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552837.1">
        <property type="nucleotide sequence ID" value="XM_002552791.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DG57"/>
    <dbReference type="FunCoup" id="C5DG57">
        <property type="interactions" value="79"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DG57"/>
    <dbReference type="GeneID" id="8295057"/>
    <dbReference type="KEGG" id="lth:KLTH0D02574g"/>
    <dbReference type="eggNOG" id="KOG0620">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_858445_0_0_1"/>
    <dbReference type="InParanoid" id="C5DG57"/>
    <dbReference type="OMA" id="QVYTYVP"/>
    <dbReference type="OrthoDB" id="428734at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050410">
        <property type="entry name" value="CCR4/nocturin_mRNA_transcr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121">
        <property type="entry name" value="CARBON CATABOLITE REPRESSOR PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121:SF11">
        <property type="entry name" value="RNA EXONUCLEASE NGL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="1">
        <location>
            <begin position="33"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="368" mass="43078" checksum="71BF794ACA89D2A9" modified="2009-07-28" version="1">MFTRKLLPVSNSALKPSLPEIRNSVSRSTFTLMTYNMLSPYYMWPQVYTYVPEKYKDWSYRHRLLEREILNLYRADIMCVQELTCKDYEGFWKNHFKTKMNYGSSYIAKTPPKYWKRPEDEMDGVGIFYNLNKFEHISTSSIYLNDLIGLFNISELNYLKSTIITLTNGAGEPIDKDSLLNVLHGRNQVSLFVSLLHKETRTLFVVVNTHLYWKYDEVKLCQCLTIMRKLHRVIKSLLMGMEGVTYSKVKILFAGDLNSAPDSPVIKFLKGESVHRADLSLINPLRPYLNRYIYQDTSADLFEHTCYSGKLKGIFDYIWYHDKDFRLKRILSGAEVSQELRELQEFGLPNKDHPSDHIPVLAELELLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DG63</accession>
    <name>C5DG63_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0D02706p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0D02706g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the large ribosomal subunit (LSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes). The 5 acidic ribosomal P-proteins form the stalk structure of the 60S subunit. They are organized as a pentameric complex in which uL10/P0 interacts with 2 heterodimers, P1A-P2B and P1B-P2A.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic ribosomal protein P1/P2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552843.1">
        <property type="nucleotide sequence ID" value="XM_002552797.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DG63"/>
    <dbReference type="FunCoup" id="C5DG63">
        <property type="interactions" value="771"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DG63"/>
    <dbReference type="GeneID" id="8295063"/>
    <dbReference type="KEGG" id="lth:KLTH0D02706g"/>
    <dbReference type="eggNOG" id="KOG3449">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114656_0_1_1"/>
    <dbReference type="InParanoid" id="C5DG63"/>
    <dbReference type="OMA" id="THIVFPY"/>
    <dbReference type="OrthoDB" id="1227494at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002182">
        <property type="term" value="P:cytoplasmic translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05833">
        <property type="entry name" value="Ribosomal_P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1410:FF:000002">
        <property type="entry name" value="60S acidic ribosomal protein P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01478">
        <property type="entry name" value="Ribosomal_L12_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038716">
        <property type="entry name" value="P1/P2_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027534">
        <property type="entry name" value="Ribosomal_P1/P2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044076">
        <property type="entry name" value="Ribosomal_P2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21141">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21141:SF103">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN P2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00428">
        <property type="entry name" value="Ribosomal_60s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="65"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="66"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="84"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00063629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR22405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="107" mass="10676" checksum="1CED49E28ED5CAF7" modified="2009-07-28" version="1">MKYLAAYLLLNAAGNSAPSAENVKTVLESVGIEVEEDKVSSLVSSLEGKSVEELVAEGNEKLSAVPAASGSGAPAAGAAAGGEAAEEDAPEEAAEESDDDMGFGLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGE6</accession>
    <name>C5DGE6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0D04686p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0D04686g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SOS response-associated peptidase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552926.1">
        <property type="nucleotide sequence ID" value="XM_002552880.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGE6"/>
    <dbReference type="FunCoup" id="C5DGE6">
        <property type="interactions" value="656"/>
    </dbReference>
    <dbReference type="GeneID" id="8295152"/>
    <dbReference type="KEGG" id="lth:KLTH0D04686g"/>
    <dbReference type="eggNOG" id="KOG2618">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035990_0_1_1"/>
    <dbReference type="InParanoid" id="C5DGE6"/>
    <dbReference type="OMA" id="KIMSWLH"/>
    <dbReference type="OrthoDB" id="2111841at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106300">
        <property type="term" value="P:protein-DNA covalent cross-linking repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1680.10">
        <property type="entry name" value="SOS response associated peptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003738">
        <property type="entry name" value="SRAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036590">
        <property type="entry name" value="SRAP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13604:SF0">
        <property type="entry name" value="ABASIC SITE PROCESSING PROTEIN HMCES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13604">
        <property type="entry name" value="DC12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02586">
        <property type="entry name" value="SRAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143081">
        <property type="entry name" value="BB1717-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0190">Covalent protein-DNA linkage</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="245"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="253"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR22488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="335" mass="38949" checksum="B6DB18C2516C7814" modified="2009-07-28" version="1">MCGRYALGYSGIDVGRRLTELDITYESPEDGPVNFESSYNIAPTQRAPVLTNHEHLRSMQWGLVPHWTKDVAKSQPYKTFNARKENIATSKMWATPCNYKRCVVPVSGYYEWQTKGKTKIPYYITRKDRELMFLAGMYDHVEAQDFYSYTIITGPAPPELEWLHFRMPVVLERGSKEWNMWLDESKTSWKESELEQTLKAYCDKSVLEWWQVSSEVGKVANNGKCLVSPAKGAVRDFFKKEDKTKKSLVKGEQSSRSDFESSWKHEEKDDKKPSLHERDENSQKHSKEEPRKLEEASDVKQEPEVSLKSDLNQKTTSKRGIENMLRGSINKRQKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGF0</accession>
    <name>C5DGF0_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)</fullName>
            <ecNumber evidence="4">5.4.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">KLTH0D04796g</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>O-phospho-L-seryl-[protein] + alpha-D-glucose 1-phosphate = alpha-D-glucose 1,6-bisphosphate + L-seryl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:68748"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:58392"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>alpha-D-glucose 1,6-bisphosphate + L-seryl-[protein] = O-phospho-L-seryl-[protein] + alpha-D-glucose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:68752"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:58225"/>
            <dbReference type="ChEBI" id="CHEBI:58392"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>alpha-D-glucose 1-phosphate = alpha-D-glucose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:23536"/>
            <dbReference type="ChEBI" id="CHEBI:58225"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="EC" id="5.4.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.2" evidence="4"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552930.1">
        <property type="nucleotide sequence ID" value="XM_002552884.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGF0"/>
    <dbReference type="FunCoup" id="C5DGF0">
        <property type="interactions" value="687"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGF0"/>
    <dbReference type="GeneID" id="8295157"/>
    <dbReference type="KEGG" id="lth:KLTH0D04796g"/>
    <dbReference type="eggNOG" id="KOG0625">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009330_0_1_1"/>
    <dbReference type="InParanoid" id="C5DGF0"/>
    <dbReference type="OMA" id="WIQDRAN"/>
    <dbReference type="OrthoDB" id="2291at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004614">
        <property type="term" value="F:phosphoglucomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019388">
        <property type="term" value="P:galactose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019255">
        <property type="term" value="P:glucose 1-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051156">
        <property type="term" value="P:glucose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006011">
        <property type="term" value="P:UDP-alpha-D-glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.50:FF:000002">
        <property type="entry name" value="Phosphoglucomutase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000004">
        <property type="entry name" value="Phosphoglucomutase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000005">
        <property type="entry name" value="Phosphoglucomutase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000006">
        <property type="entry name" value="Phosphoglucomutase PgmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.120.10">
        <property type="entry name" value="Alpha-D-Glucose-1,6-Bisphosphate, subunit A, domain 3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.50">
        <property type="entry name" value="Alpha-D-phosphohexomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005845">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005846">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016066">
        <property type="entry name" value="A-D-PHexomutase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005841">
        <property type="entry name" value="Alpha-D-phosphohexomutase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045244">
        <property type="entry name" value="PGM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005737">
        <property type="entry name" value="PRK07564.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22573:SF2">
        <property type="entry name" value="PHOSPHOGLUCOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22573">
        <property type="entry name" value="PHOSPHOHEXOMUTASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24947">
        <property type="entry name" value="PGM1_C_vert_fung"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02879">
        <property type="entry name" value="PGM_PMM_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02880">
        <property type="entry name" value="PGM_PMM_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00509">
        <property type="entry name" value="PGMPMM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="Phosphoglucomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="Phosphoglucomutase, first 3 domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00710">
        <property type="entry name" value="PGM_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-0313">Glucose metabolism</keyword>
    <keyword evidence="9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="15">
        <location>
            <begin position="16"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="16">
        <location>
            <begin position="206"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="17">
        <location>
            <begin position="312"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAR22492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="570" mass="62680" checksum="571F4542695D1CDC" modified="2009-07-28" version="1">MSLQIETVPTKPYNDQKPGTSGLRKKTKVFMNEPHYTENFIQAIMEAIPEGSQDAVLVVGGDGRYYNNEVLQKIGEIGAANGVKKLIVGQNGLLSTPAASHIIRSYPEKSTGGIILTASHNPGGPENDFGIKYNLSNGGPAPESVTNSIFEKSKQLASYKTVKNFPKINLSKIGKNQEYGSLLVDVIDVTAEYVKLMKEIFDFDLIKKFIDHQRSTKGFKILFDALNGITGPYGKAIFADELGLPAEEVLQNYEPKPDFGGLHPDPNLTYAHTLVERVDKEQIAFGAASDGDGDRNMIYGCGPAFVSPGDSVAIIAEYASEIPYFKKQGIYGLARSFPTSAAIDRVAKAQGLNCYEVPTGWKFFCALFDAKKLSICGEESFGTGSNHVREKDGVWAIVAWLNILAIYNREHPDSDVSIKVVLDEFWAKYGRTFFTRYDFEQVSGEAAAKVLRVLEDLVVSKESAIGKLLPGNTDLTVTDCGDFSYTDLDGSVSEHQGLYAKLSNGCRFVVRLSGTGSSGATIRLYLERYTDDASKYSLNVEQFMKDDIKAVLKFLKFREYLGTDEPTVRT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-06-28" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGV3</accession>
    <name>EIS1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName>Eisosome protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">EIS1</name>
        <name type="ordered locus">KLTH0D08558g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for normal formation of eisosomes, large cytoplasmic protein assemblies that localize to specialized domains on plasma membrane and mark the site of endocytosis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic granule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <text evidence="1">Localizes at the eisosomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the EIS1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGV3"/>
    <dbReference type="SMR" id="C5DGV3"/>
    <dbReference type="FunCoup" id="C5DGV3">
        <property type="interactions" value="73"/>
    </dbReference>
    <dbReference type="KEGG" id="lth:KLTH0D08558g"/>
    <dbReference type="eggNOG" id="ENOG502S8WV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013228_1_0_1"/>
    <dbReference type="InParanoid" id="C5DGV3"/>
    <dbReference type="OMA" id="EHTFSGF"/>
    <dbReference type="OrthoDB" id="4070583at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070941">
        <property type="term" value="P:eisosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024527">
        <property type="entry name" value="Eisosome1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28298">
        <property type="entry name" value="EISOSOME PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28298:SF1">
        <property type="entry name" value="EISOSOME PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12757">
        <property type="entry name" value="Eisosome1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000410801" description="Eisosome protein 1">
        <location>
            <begin position="1"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="98"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="516"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="637"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="13"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="98"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="523"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="676"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="703"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="721"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="740"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="781"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="849"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="883"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="898"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="919"/>
            <end position="930"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="930" mass="101460" checksum="03BD1F9EBA17E557" modified="2009-07-28" version="1">MSLISAAADVNDASSTTSAGSVRSSAVYRKDGKPLSQEALYRAQQKYGVFQSPARQTGSGLKDSKMASDVAANLANNNRTTIEAYKRVLDSNASRAATAVSSRSRSSSVTSNATVVTTSSKSTNAAVKALSSKPVEQPVAPKKSNMNMSKILVGAEAAAEKRIGIRMKPEKIVYVPSKESGKAAERSMSLTPEIMDKLKTKGDYEAEAEVEADPKKYASKAAFAVRDFDPNEATEKELLEREKKKQAYFGMLTSPQVLSLARANAQVKLDQIDKAAPGSLYKNEEFNKLAVALAQKNSTKRSEHHGKINMGGGLWLTQADVQNIAQGLITPVLDEVDSRALQQRAIDEDIKQRKIDFKEQNAAWIELQRNKLSNDKMYSRETRMRHKRETEGLHARTERKFQDLCASKDSEVAEMEKALQNAKDSYAALQKQMEEDLEKERLRVEAEVAALKKEQEEDLKAARVEQEQELKPYVDDVKAAEAEHERLTAERDSLNKEIEELRASIESHKVRIEELDNEISDSAAKHEEEEGKREELTKHKEEFDQEVSEKFTVIAQAAKEKAQKSSEEARLKQLEVDAMINERQSELNSTELELKKEKLSLLEAMRNVTELKGEDKLDENRVKALIGMTSEEFIAENNKSVNVSDDKFEPFNEHTASTKSIKHEEGVLGEGGAKTNSDELPVKDSAEKSSEHLNSTAEKSIEPAKASSPYPAKPSMVDAVLPKDFKPEVKPKAKPAHKTPQQGAAAGGEPASAKSGVKRSPSLKQKFMGIIKGDTKSQSKPAAAATPVHPPATVKKAAPKKAAASSEADTPAKVSPAKDTAKTEIQKIAQGGPAPAPEPEHKTAPAVDNATTKSVSTLQKPTNSGIDKSEIHKIAQGGPAPTSGHSNHTRTSVYENGDNSDDEDELPDSSEAGENGIGADKKGSLFKEVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHG8</accession>
    <name>C5DHG8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0E04224p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0E04224g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553666.1">
        <property type="nucleotide sequence ID" value="XM_002553620.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DHG8">
        <property type="interactions" value="95"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHG8"/>
    <dbReference type="GeneID" id="8291815"/>
    <dbReference type="KEGG" id="lth:KLTH0E04224g"/>
    <dbReference type="eggNOG" id="ENOG502QQWJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003023_1_0_1"/>
    <dbReference type="InParanoid" id="C5DHG8"/>
    <dbReference type="OMA" id="VLKSPKW"/>
    <dbReference type="OrthoDB" id="2015333at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052811">
        <property type="entry name" value="Glucose_resp_signaling"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014770">
        <property type="entry name" value="Munc13_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014772">
        <property type="entry name" value="Munc13_dom-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057984">
        <property type="entry name" value="PATROL1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47263">
        <property type="entry name" value="ADENYLATE CYCLASE ACTIVATION PROTEIN GIT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47263:SF1">
        <property type="entry name" value="C2 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_7G02350)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25761">
        <property type="entry name" value="TPR_PATROL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51258">
        <property type="entry name" value="MHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51259">
        <property type="entry name" value="MHD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MHD1" evidence="3">
        <location>
            <begin position="622"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="2">
        <location>
            <begin position="845"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="domain" description="MHD2" evidence="4">
        <location>
            <begin position="1048"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR23229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1308" mass="148756" checksum="ECA2528ABA6E7512" modified="2009-07-28" version="1">MAARHVSASSTISSGSTSNLLMSGAPVPSMTQPELDAILHYTLKVILIEYINEPRFKIRAVKPRGPVRTASKSTKEKRNKRSSWLFSDDGARKDQAEAKQLQKLTPTLENYLRMVAIDKIKVKQTQLRRSLLKLYNDAFLDPHASRSLLSLTRLEELVMLFTKAANGELAKVGNTSKTKEELYSQVCCFLDTITQLATQQESSKSRLVTRIHEYKKQFSNPKSMEPLFSSLPSNGSNTELSEPAVKPTFKLSDVAHSAYLMELFNMSEIELQQILIKNVNETNNSIFGRSMRALKAKVALQDNTESAYAFPITKSFEEWKTSEMQELDALIAKFGRHGASEYEATDLSRSIPEKARDLYTGLARLILEKELSAGPATSILPKDAVFLLTKCAYYWRVDLSSTKATLLYTAANAGPLSGETNASEKFEMLFKIISTKALSKEEDLGSLPTWRIIDQQEWMSNLTITFEQCMNSINLCLSAIYQKLMPKFSPILSIYYTYIEPGFELLGHNIAETNLFKKHSRRLHRVLFRTSEQYYVSLVKGLPRDGSLEIHNVKHIADQILKNLQDIQKKYKNPLLDQINLASECAKVLIEAFGVDCSAMLAQVERFSKAENQNKIPLYDATETYKTLRELRDVYAQVQPSKKFPFKLEKHFLKYLSELCDDCCSGTLAVVKTALEKEDWEPADMSETYSTSVIDIFKMIHESFDLFLKLGWADQYQISKICTFLLKSVADGLSYYANTVANLVESEMNISQSEEGEFAETENERKVLDVISQTAAAKMKNTWLYSEMRSALKSSDVVIPKPYEFQSKTCTCLNNLNQMMQMISDLEERINPEEMSSVVNQHERLDAKRRMMTDRQNKGLKHLYTVKIIRAENVQVTSHNAGLLSTVSIVDAKNKREVAKTREVLKASRPVWNEEFEIEIPVNDTRPFSFILWHHKPKSDSTSSRMSGRSFLYLDPHKFKNDGYPQDVCLNLDTHGQLFLQISVETERVDALFAMGRAFRSLSRARDRSIELMVSKFQVFVHFAFSRTTLKTICGANGQIRPSNTEIYDSIVPLFDYLNANLNILASRLSRELLHKIMLEAWEEILNEADALLLPFLSDAPRAYQRSVKNKSIWENAVDVAKLGMSASSGSERPLTQIEIDTVFEWLRALCIDFFHNNGEGPPLTDLKNMHYQALLLIPVFYDKNVSELKNEADALNRDYRKSMFGVNNNDADGPRRANSNLRRANTLARKRTIIANSSRKRRLEVDKEIKASEENPAENIVSTQDVILRILIAKGEAEFVLESLSLRERVSKALRTEQIVKAAKKSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHH5</accession>
    <name>C5DHH5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0E04378p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0E04378g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553673.1">
        <property type="nucleotide sequence ID" value="XM_002553627.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHH5"/>
    <dbReference type="FunCoup" id="C5DHH5">
        <property type="interactions" value="187"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHH5"/>
    <dbReference type="GeneID" id="8291822"/>
    <dbReference type="KEGG" id="lth:KLTH0E04378g"/>
    <dbReference type="eggNOG" id="KOG2693">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015114_0_0_1"/>
    <dbReference type="InParanoid" id="C5DHH5"/>
    <dbReference type="OMA" id="IWLHSIG"/>
    <dbReference type="OrthoDB" id="200954at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006882">
        <property type="term" value="P:intracellular zinc ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16950">
        <property type="entry name" value="ZINC TRANSPORTER SLC39A7 HISTIDINE-RICH MEMBRANE PROTEIN KE4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16950:SF16">
        <property type="entry name" value="ZINC TRANSPORTER ZIP13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012587520" evidence="7">
        <location>
            <begin position="16"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="48"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="78"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="151"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="380"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="419"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="220"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="231"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR23236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="437" mass="46591" checksum="BB913868AF603EBB" modified="2009-07-28" version="1">MMYAALLSLLPKVMAHPGHHHGTSVMHGEVKDSVIAQFLFPYSARVNALLATVYISAAPCLIVTAIPELRKAKRGSLLSLLMSFAFGTLMGDIILHLLPEIFSEAIDVNEEAQWLKFLDGLPLQDSTNGHVKDLLVTLKPSLTSHNSPSKMRTMALGFLVFCGFVIFMVIDKGLKIANSGGEHEEGHHHGHSHIFSVQHEKEAEEGVEAVSSGKVVTSDEGMNVVRRSKGNKTEGSDVDDDIGHERAPPQSSSLKASAYLSLVSAFAHNITDGVALASAFYKSKHTGIVTTLAVLMHEIPHELGDFAILLASGINFNQALLLQLVGAFGALIGTLCGCIANEMAAGTADTGIASRIIPEIGFPTFVAHASSKWPISAGELMLPITTGGLLFISTVGVVPELLKVTGSSKKNELTNSLRHLGSVFAGFVLMAWMGLNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHN0</accession>
    <name>C5DHN0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0E05676p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0E05676g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MIP/aquaporin (TC 1.A.8) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553728.1">
        <property type="nucleotide sequence ID" value="XM_002553682.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHN0"/>
    <dbReference type="FunCoup" id="C5DHN0">
        <property type="interactions" value="41"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHN0"/>
    <dbReference type="GeneID" id="8291881"/>
    <dbReference type="KEGG" id="lth:KLTH0E05676g"/>
    <dbReference type="eggNOG" id="KOG0224">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027014_0_0_1"/>
    <dbReference type="InParanoid" id="C5DHN0"/>
    <dbReference type="OMA" id="MGTMVMI"/>
    <dbReference type="OrthoDB" id="3222at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015254">
        <property type="term" value="F:glycerol channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015250">
        <property type="term" value="F:water channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00333">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050363">
        <property type="entry name" value="MIP/Aquaporin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022357">
        <property type="entry name" value="MIP_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00861">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43829">
        <property type="entry name" value="AQUAPORIN OR AQUAGLYCEROPORIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43829:SF9">
        <property type="entry name" value="AQUAPORIN-9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00221">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="200"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="312"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="396"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="448"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="89"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="136"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="511"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="29"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="100"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="136"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="574"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="605"/>
            <end position="614"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR23291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="614" mass="67377" checksum="31C7CB2E609B2066" modified="2009-07-28" version="1">MSKKSLPDSQDGNQRKPQEYMNDFLSDAESGSNVSHDQQNGGDGGEHYIPSRDFAPQHRISASASYVPRNGEGGGGSNYAVQQVVPNTHIPMSRSNSGNNGSGGGGGGGGGSGHSTGYRNRAQSGVSSNYMNMRSVQSNSGSAQNVNQPEASADPRDNDIPLMVKPKTLYQNPQTPTVLPSTYHPINTWSTLKQTYLKEFLAEFMGTMVMIFFGCTVVCQVRSGQQQQRVTFLKELSASTQVPAENKIELLQYLMPVDQFGTYDDVALIWGGAVVMGYFAAGGSALSGGHMNPALTLSNCVFRGFPWRKVPVYWAGQLLGAYCGALIVFAYYMPVIKYVYPDWNGNETVLGMFCTSPLQYLDSSRQFISELICSAVLQVGIFALTDPYTCLRSDLFPLMLFVLIFCLIGATALQTGAGLNPARDLGPRLALATVGFESDTLWRAHHHYFWVPIVAPFVGTLLGGTVYDICIYQGHESPLNWPYSLVKHKARRAWSNRPRFYRKRAGSDSAVSDWEYDNESNNASARDTDNEDTPKTGFFPESDPQIQKHVQFKSMSKNFNGKRNPVSGIPTIFEEGDDDDVDDADEAASGNETEKRPPLKPKSSKSFDKKGKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHT9</accession>
    <name>C5DHT9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 10">5'-3' exoribonuclease 1</fullName>
            <ecNumber evidence="10">3.1.13.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">KLTH0E07062g</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Multifunctional protein that exhibits several independent functions at different levels of the cellular processes. 5'-3' exonuclease component of the nonsense-mediated mRNA decay (NMD) which is a highly conserved mRNA degradation pathway, an RNA surveillance system whose role is to identify and rid cells of mRNA with premature termination codons and thus prevents accumulation of potentially harmful truncated proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Perinuclear region</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 10">Belongs to the 5'-3' exonuclease family.</text>
    </comment>
    <dbReference type="EC" id="3.1.13.-" evidence="10"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553787.1">
        <property type="nucleotide sequence ID" value="XM_002553741.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DHT9">
        <property type="interactions" value="1094"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHT9"/>
    <dbReference type="GeneID" id="8291942"/>
    <dbReference type="KEGG" id="lth:KLTH0E07062g"/>
    <dbReference type="eggNOG" id="KOG2045">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001581_1_2_1"/>
    <dbReference type="InParanoid" id="C5DHT9"/>
    <dbReference type="OMA" id="VASWPWF"/>
    <dbReference type="OrthoDB" id="372487at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004534">
        <property type="term" value="F:5'-3' RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016078">
        <property type="term" value="P:tRNA decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18673">
        <property type="entry name" value="PIN_XRN1-2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.1050:FF:000001">
        <property type="entry name" value="5'-3' exoribonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.750:FF:000002">
        <property type="entry name" value="5'-3' exoribonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12390:FF:000002">
        <property type="entry name" value="5'-3' exoribonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12390">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027073">
        <property type="entry name" value="5_3_exoribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016494">
        <property type="entry name" value="5_3_exoribonuclease_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041385">
        <property type="entry name" value="SH3_12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040992">
        <property type="entry name" value="XRN1_D1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047007">
        <property type="entry name" value="XRN1_D1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041106">
        <property type="entry name" value="XRN1_D2_D3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041412">
        <property type="entry name" value="Xrn1_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004859">
        <property type="entry name" value="Xrn1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047008">
        <property type="entry name" value="XRN1_SH3_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12341:SF7">
        <property type="entry name" value="5'-3' EXORIBONUCLEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12341">
        <property type="entry name" value="5'-&gt;3' EXORIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18129">
        <property type="entry name" value="SH3_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18332">
        <property type="entry name" value="XRN1_D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18334">
        <property type="entry name" value="XRN1_D2_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17846">
        <property type="entry name" value="XRN_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03159">
        <property type="entry name" value="XRN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006743">
        <property type="entry name" value="Exonuclease_Xnr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 10" id="KW-0866">Nonsense-mediated mRNA decay</keyword>
    <keyword evidence="3 10" id="KW-0540">Nuclease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Xrn1 N-terminal" evidence="12">
        <location>
            <begin position="1"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="Xrn1 helical" evidence="13">
        <location>
            <begin position="271"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="domain" description="5'-3' exoribonuclease 1 D1" evidence="15">
        <location>
            <begin position="722"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="domain" description="Exoribonuclease Xrn1 D2/D3" evidence="16">
        <location>
            <begin position="920"/>
            <end position="1147"/>
        </location>
    </feature>
    <feature type="domain" description="5'-3' exoribonuclease 1 SH3-like" evidence="14">
        <location>
            <begin position="1165"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1247"/>
            <end position="1327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1427"/>
            <end position="1496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1280"/>
            <end position="1299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1300"/>
            <end position="1326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="1463"/>
            <end position="1481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00067318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR006743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF03159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF17846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF18129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF18332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF18334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAR23350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1496" mass="170333" checksum="8A43BF6ED121543E" modified="2009-07-28" version="1">MGIPKFFRYISERWPMISQLIDGTQIPEFDNLYLDMNSILHTCTHANDDDVKKRMSEEEVFSKIFAYIDHLFHTIGPKQTFYMAIDGVAPRAKMNQQRARRFRTAMDAEKALEKAIRDGEEIPKGEPFDSNSITPGTEFMAKLTENLKYFIHDKISNDSAWSSTSIILSGHEVPGEGEHKIMEYIRRMRAQPDYNANTRHCIYGLDADLIMLGLSTHDPHFALLREEVTFGRRQKAAPLEYQNFYLLHISMLREYLELEFREIADDLQFEYDFERILDDFILVMFVIGNDFLPNLPDLHLNKGAFPVLLQTFKEALKHTDGYINEQGTINLSRFQVWLQYLSEFEMMNFEREDIDVEWFNKQLENISLEGERKRARVGKKLLLKQQKKIVGMIKPWLLKTASHPLDVNNISEDQIPTLTLGDEIIKENLSFLKEVAFDLGLFVAHSQSKDNYFLQLDVDGINPHESQEERDQRIMSLRKSIKKYEQAILVEDSEELEEEQKIYNERFEKWRDQYYREKLGFSYYDEDKLRELTETYVEGLQWVLFYYYRGCQSWSWYYKYHYAPRISDVYKGLNQSIKFNLGKPFRPFQQLMAVLPERSKNLIPTVYRPLMYDPKSPIADFYPSEVELDKNGKTADWEAVVKLSFVDENRLIEAMAPYDEKLTPEEKRRNSFGTDLAFVFNPQVDDVYKSPLSGIFSDIEHNHCTEREFVPDSMEGLDFLFGLPTGAKLGVEALAGFPTLKTLAFDNKLDYNGCMIFQQPSRQQSMLLSIRDAYKAGNLTLDEVAKTHLGKVIYTRWPNLRESKLLSITDGKTTFEASEYTDARGIHHIKISQKQTEEAERKLFNSQKSSMMRTYSINRGVILDNIRAIVKVVPVNGLTRTIEGAYTKTFSPVEEYYPLQLVVEDVENKDERFMERPPLPIDEEFPQGSDVIFLGDYAYGGKATIDGYSSPTRLKLTVEKSVTKSEPNVGKVRAKLDEKMIRYYPSFVVAKKLQIHPLFLSRITSKFLVSGLKDKSVDFGLKIKFESRHEKVLGYARRNVRGWEYSDMTVALLSEYKRNFPDLFSKLSDAGRDVPSISKLYPGVDANDLRKLIDDVSSWLKNVRQNFVTVSLESDSLTKASMIAVEDAIEKYVTNPVDVSKKQLAKVPREAVLDPKESFSLLRTQKFDLGDRVIYIQDSGKVPLFSKGTVVGYTTIGPKLSIQVLFDHEIVAGNNFGGRLRTKRGLGLDSSFLLNLSNRQFIYHSKASKSAKETGKNNGKAPKKNSEKGGHAKSATPPTEDLKKKQAHELLTHIQKKEPSSGNKENSDTNTSQGRDANKVNPQVGSQDDRVIANNIYNAVFNQFSGPGTAAPPQQFPHGLPYNAHASGAVPPQFVGNFAPPGYYPMPPQAGIPIMSPVMNGQGQQIPVNALGTQQVAELLKKRDGASPEMNFAAPNGENFEPADHNGHGLPSRGRGYSGRGRGSGRGRGRGRGSGRGRGSFRGRGSDRSGVIAAEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>C5DI55</accession>
    <name>C5DI55_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Checkpoint forkhead associated with RING domains-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ordered locus">KLTH0E09834g</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the DMA1 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553903.1">
        <property type="nucleotide sequence ID" value="XM_002553857.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DI55"/>
    <dbReference type="FunCoup" id="C5DI55">
        <property type="interactions" value="176"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DI55"/>
    <dbReference type="GeneID" id="8292067"/>
    <dbReference type="KEGG" id="lth:KLTH0E09834g"/>
    <dbReference type="eggNOG" id="KOG3872">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017542_2_0_1"/>
    <dbReference type="InParanoid" id="C5DI55"/>
    <dbReference type="OMA" id="SHSWHYQ"/>
    <dbReference type="OrthoDB" id="687730at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032186">
        <property type="term" value="P:cellular bud neck septin ring organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000132">
        <property type="term" value="P:establishment of mitotic spindle orientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031578">
        <property type="term" value="P:mitotic spindle orientation checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051865">
        <property type="term" value="P:protein autoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097271">
        <property type="term" value="P:protein localization to bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090337">
        <property type="term" value="P:regulation of formin-nucleated actin cable assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000921">
        <property type="term" value="P:septin ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16458">
        <property type="entry name" value="RING-H2_Dmap-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000426">
        <property type="entry name" value="DMA1p Ubiquitin-protein ligase (E3)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.20:FF:000030">
        <property type="entry name" value="FHA domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042823">
        <property type="entry name" value="Dma1/Dma2_RING-H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15067:SF7">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE DMA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15067">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17123">
        <property type="entry name" value="zf-RING_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="13" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="11" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 17" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FHA" evidence="19">
        <location>
            <begin position="260"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="20">
        <location>
            <begin position="398"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="26"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="46"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="103"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00061209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00080465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="CAR23466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="479" mass="52865" checksum="B5B08BF17FBF5A08" modified="2009-07-28" version="1">MVVNISPRNSLSSRSRRGSTINNLLNSMGIRQGSNGPGMAPIPAGSSGTTEPLNNNGDMAATGPSRATEPFNSATFARSNEGREDMAESNAIEELSSDDDEVNGDRDRVLRDENGAPTAANMLGTTPFDQGIQNSSVPLSVNNLVSELPITLTLGQNDGPGGAGQVSTLTPNESQMVPKHVRHFVYGNNQPEPALDLLGLELPRNAAIPEPVSEEVIQSRKDKSGLFSLRLTPFLDQSASTNPGLYFEPIVRTAGPCSQLVIGRYTERVRESINRIPEHFHPVVFKSKVVSRTHGCFKVDQQGNWYIRDVRSSSGTFLNHHRLAPASTMSKDTPIQDGDVIQLGLDFRGGTEEIYRSVKMRVELNRSWKRRAMRFNREALQRLKNLQKMTSGLEEEDCSICLSKIKPCQAIFISPCSHSWHYQCVRRLVMTTYPQFVCPNCRSSCDLEASLDSDMDSDENDFEVLADDVKDIDMAQESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DI92</accession>
    <name>C5DI92_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Riboflavin kinase</fullName>
            <ecNumber evidence="4">2.7.1.26</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Flavin mononucleotide kinase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0E10670g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN) coenzyme.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (ATP route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the flavokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.26" evidence="4"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553940.1">
        <property type="nucleotide sequence ID" value="XM_002553894.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DI92"/>
    <dbReference type="FunCoup" id="C5DI92">
        <property type="interactions" value="404"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DI92"/>
    <dbReference type="GeneID" id="8292105"/>
    <dbReference type="KEGG" id="lth:KLTH0E10670g"/>
    <dbReference type="eggNOG" id="KOG3110">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048437_3_2_1"/>
    <dbReference type="InParanoid" id="C5DI92"/>
    <dbReference type="OMA" id="NGEVHKM"/>
    <dbReference type="OrthoDB" id="276388at2759"/>
    <dbReference type="UniPathway" id="UPA00276">
        <property type="reaction ID" value="UER00406"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008531">
        <property type="term" value="F:riboflavin kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009398">
        <property type="term" value="P:FMN biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.30">
        <property type="entry name" value="Riboflavin kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023468">
        <property type="entry name" value="Riboflavin_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015865">
        <property type="entry name" value="Riboflavin_kinase_bac/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023465">
        <property type="entry name" value="Riboflavin_kinase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22749:SF6">
        <property type="entry name" value="RIBOFLAVIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22749">
        <property type="entry name" value="RIBOFLAVIN KINASE/FMN ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01687">
        <property type="entry name" value="Flavokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00904">
        <property type="entry name" value="Flavokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82114">
        <property type="entry name" value="Riboflavin kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0288">FMN</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Riboflavin kinase" evidence="12">
        <location>
            <begin position="12"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR23503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="184" mass="20653" checksum="E59F0F24C2EC811E" modified="2009-07-28" version="1">MPRTADVPIPDFPQNPYPITTEFCDIVCGFGRGSSELGVPTANVLFEQVPKVVESLPLGVFFGFARLVQAKKGSKRTLRRPDGSHVDFNFGQSLSDEEMQVWPVVLSIGLNPFYQNDTKTVELHIIHKFGHDFYGAKVKFNILGYIRPELDYTTKEALIKDIKTDIKIATNTLAKPGYKAYNKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-04-02" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIB0</accession>
    <name>C5DIB0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0E11088p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0E11088g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Nucleoside permease that transports adenosine and guanosine.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NUP family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553958.1">
        <property type="nucleotide sequence ID" value="XM_002553912.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIB0"/>
    <dbReference type="STRING" id="559295.C5DIB0"/>
    <dbReference type="GeneID" id="8292124"/>
    <dbReference type="KEGG" id="lth:KLTH0E11088g"/>
    <dbReference type="eggNOG" id="ENOG502QQPU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031475_0_1_1"/>
    <dbReference type="InParanoid" id="C5DIB0"/>
    <dbReference type="OMA" id="WAHYLVE"/>
    <dbReference type="OrthoDB" id="2331083at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009116">
        <property type="term" value="P:nucleoside metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="entry name" value="Nucleoside phosphorylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009486">
        <property type="entry name" value="Pur_nuclsid_perm"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38643">
        <property type="entry name" value="PURINE NUCLEOSIDE PERMEASE C285.05-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38643:SF1">
        <property type="entry name" value="PURINE NUCLEOSIDE PERMEASE C285.05-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06516">
        <property type="entry name" value="NUP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF013171">
        <property type="entry name" value="Pur_nuclsid_perm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950491" evidence="2">
        <location>
            <begin position="19"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR013171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR23521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="401" mass="43896" checksum="2EC5DC7FC2F60EA0" modified="2009-07-28" version="1">MKLRTVCSALWAASLAAANPISRKESNATASVSSYSQTASIADAASTTDTIFSVPQGRYGALFKPKVMVVNMYYKEEDAWIRNLDLMHNISVPLLSPEYPYVHSNDNFTIMSVTTGEGEINAATTIAALCMSPLLDLTETYFLISGIGGGSPQHTTIGSVTFAKYAVQVGLQYQIDSREIPANWTYGYVNFKTSQPNVYPATVYGTEIFELNENLMNRAMHLASNVTLQNGTTGNVSFRQRYNASMAAYGAPKLVACDTATSDVYWSGEILEHAMSDFVTTISNKTATYCSTQQEDNASLESFLRAAKHGLVDYNRIVLTRGISDFDRAPNGLNATEFFFGGKNLQGGATATFTNLYLAGLPFVQDVVANWDTLYKENNFMPKNYIGDYFETLGGVKNFGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIL8</accession>
    <name>C5DIL8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0E13530p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0E13530g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554066.1">
        <property type="nucleotide sequence ID" value="XM_002554020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIL8"/>
    <dbReference type="FunCoup" id="C5DIL8">
        <property type="interactions" value="671"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIL8"/>
    <dbReference type="GeneID" id="8292235"/>
    <dbReference type="KEGG" id="lth:KLTH0E13530g"/>
    <dbReference type="eggNOG" id="KOG2411">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014330_4_1_1"/>
    <dbReference type="InParanoid" id="C5DIL8"/>
    <dbReference type="OMA" id="DWPLLEW"/>
    <dbReference type="OrthoDB" id="439710at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004815">
        <property type="term" value="F:aspartate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006422">
        <property type="term" value="P:aspartyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.30">
        <property type="entry name" value="GAD-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00044">
        <property type="entry name" value="Asp_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004524">
        <property type="entry name" value="Asp-tRNA-ligase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004115">
        <property type="entry name" value="GAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00459">
        <property type="entry name" value="aspS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001750">
        <property type="entry name" value="PRK00476.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594:SF5">
        <property type="entry name" value="ASPARTATE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594">
        <property type="entry name" value="ASPARTYL/LYSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="7">
        <location>
            <begin position="206"/>
            <end position="654"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR23629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="673" mass="76581" checksum="BDAEF5A69528C4E4" modified="2009-07-28" version="1">MYSFQSGLLPLKNEVPKISFVSFTISLVTNNYTSMMLRSRLVLVRQWERRYSELVNPKIIKERFKLNKEAQPIKALLNRGNSDAVVINGWIDKKPRSVGKGLIFSTLRDTEGDLIQLVDHKSLLKSASVEDVVQVEGHLALKESRGDSDPAQYELRVLNINTLNKSSKKPSQLLGFKIQESYPPEFRYLQLRLPKYQNFLQKRHQIAQNIRELLNLQGFLEVETPVLFKSTPEGAREFLVPARNKQDGKPTFYALPQSPQQYKQLLMASGIQKYYQFARCFRDEDLRSDRQPEFTQVDLEMAFASGDKIMHVVEDTMVSTWNKFSSSEALQTVDKNGLLVAVTGSQPVSRMSYKTAMTHYGIDKPDLRFRDLKIVDMSEFKARGKENILFPVFEVLILRNAFSTVEEYHKNWAFLSNEHNYRYRTPIVVPVETEELERSWFEKFLPIADFENPTLMARFLNLKKGDIVCGSTRQPTSAIFENPTPLGRLRQLAIQSPYGKKLYQKSEGAVATWVVDFPLFSPVEREVVGKETKQLYPVYESDKVTSTHHPFTMVQLDDLAKLSKAPLSCAGQHYDFVLNGVELGGGSTRIHDPELQKYVFKDILKIKEPEKLFGHLLTAFENGTPPHAGLAIGFDRMVAMICGSENIRDVIAFPKSVSGADLVVGSPSSILES</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIQ2</accession>
    <name>C5DIQ2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0E14300p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0E14300g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="5">Protein modification.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554100.1">
        <property type="nucleotide sequence ID" value="XM_002554054.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIQ2"/>
    <dbReference type="FunCoup" id="C5DIQ2">
        <property type="interactions" value="932"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIQ2"/>
    <dbReference type="GeneID" id="8292270"/>
    <dbReference type="KEGG" id="lth:KLTH0E14300g"/>
    <dbReference type="eggNOG" id="KOG0417">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030988_13_3_1"/>
    <dbReference type="InParanoid" id="C5DIQ2"/>
    <dbReference type="OMA" id="VHFTTRI"/>
    <dbReference type="OrthoDB" id="7851174at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23792">
        <property type="entry name" value="UBCc_UBE2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000001">
        <property type="entry name" value="Ubiquitin-conjugating enzyme 28, E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="8">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00043952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR23663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="147" mass="16352" checksum="101BFB4B12924CB3" modified="2009-07-28" version="1">MSLKRITKELNDLGRDPPTSCSAGPVGDDLYHWQASIMGPPDSPYAGGVFFLSIHFPTDYPFKPPKISFTTKIYHPNINANGNICLDILKDQWSPALTISKVLLSICSLLTDANPDDPLVPEIAHIYKTDRAKYEATAKEWTKKYAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIY3</accession>
    <name>C5DIY3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0E16104g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554181.1">
        <property type="nucleotide sequence ID" value="XM_002554135.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIY3"/>
    <dbReference type="FunCoup" id="C5DIY3">
        <property type="interactions" value="432"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIY3"/>
    <dbReference type="GeneID" id="8292352"/>
    <dbReference type="KEGG" id="lth:KLTH0E16104g"/>
    <dbReference type="eggNOG" id="KOG0591">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_63_23_1"/>
    <dbReference type="InParanoid" id="C5DIY3"/>
    <dbReference type="OMA" id="YKCHYGE"/>
    <dbReference type="OrthoDB" id="10250725at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044732">
        <property type="term" value="C:mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08217">
        <property type="entry name" value="STKc_Nek2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000525">
        <property type="entry name" value="Serine/threonine-protein kinase KIN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050660">
        <property type="entry name" value="NEK_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE NEK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43671:SF13">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="19"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="314"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR23744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="406" mass="47265" checksum="904A0CAE39064956" modified="2009-07-28" version="1">MYNRRPEFREYTGPRTGSYQVLEEIGRGSFGSVRKVIHLPTNKVLVRKEIKYGHMNSKERQQLISECAILSQLKHEHIVEFYEWDSDKDVLYFYMEYCSNGDLSQMISFFKKQRKYIPEKIVWSIMVQILIALYRCHYGADLAPLETIYDRTKPPVKGSNIVIHRDLKPGNIFLSTERVPDPLAAPQTLDYSKVSVKLGDFGLAKALGASVEFATTYVGTPYYMSPEVLMDQPYSPLSDIWSLGCVIYEMCTLRPPFQAKTYSELQRKIKAGNFDRIPEYYSDELQKLIESCIDPNLRTRASTYSLLQDPQLRVARKSLQLERFEDNLMDYEKELMNIGDILERQAGEYEAELAVIKYQYRKEFDAAVEQRVREVLNGKKVTGTPEIMAGRNHLHRPDYHWRVKGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIZ2</accession>
    <name>C5DIZ2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0E16324p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0E16324g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The proteasome degrades poly-ubiquitinated proteins in the cytoplasm and in the nucleus. It is essential for the regulated turnover of proteins and for the removal of misfolded proteins. The proteasome is a multicatalytic proteinase complex that is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. It has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the peptidase T1A family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554190.1">
        <property type="nucleotide sequence ID" value="XM_002554144.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIZ2"/>
    <dbReference type="FunCoup" id="C5DIZ2">
        <property type="interactions" value="1128"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIZ2"/>
    <dbReference type="GeneID" id="8292362"/>
    <dbReference type="KEGG" id="lth:KLTH0E16324g"/>
    <dbReference type="eggNOG" id="KOG0181">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035750_4_1_1"/>
    <dbReference type="InParanoid" id="C5DIZ2"/>
    <dbReference type="OMA" id="ATCIGKD"/>
    <dbReference type="OrthoDB" id="431557at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010499">
        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03750">
        <property type="entry name" value="proteasome_alpha_type_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000048">
        <property type="entry name" value="Proteasome subunit alpha type-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003075">
        <property type="entry name" value="PRK03996.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 8" id="KW-0647">Proteasome</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="9">
        <location>
            <begin position="5"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00026071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR23753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="250" mass="27160" checksum="E14CD65D22F4BB24" modified="2009-07-28" version="1">MADRYSFSLTTFSPSGKLGQIDYALTAVKQGVTSLGIRATNGVIIATEKKSTSSLALTESLSKVAHITPDIGAAYSGMGPDFRVLIDKSRKVAHTHYKRIYNEYPPTKILVAEVAKIMQEATQSGGVRPFGVSLLVAGYDEHNGYGLYQVDPSGSYFPWKATAIGKGATAAKTFLEKRWNDELELEDAIHIALLTLKESVEGEFNGDTIEIAVVGEENQDLLGFTGDPSVKGPRFRKLTAQEINDRLEAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJ00</accession>
    <name>C5DJ00_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0E16500p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0E16500g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554198.1">
        <property type="nucleotide sequence ID" value="XM_002554152.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJ00"/>
    <dbReference type="FunCoup" id="C5DJ00">
        <property type="interactions" value="343"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJ00"/>
    <dbReference type="GeneID" id="8292370"/>
    <dbReference type="KEGG" id="lth:KLTH0E16500g"/>
    <dbReference type="eggNOG" id="ENOG502QQBG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009030_1_0_1"/>
    <dbReference type="InParanoid" id="C5DJ00"/>
    <dbReference type="OMA" id="ARQIMFE"/>
    <dbReference type="OrthoDB" id="3477330at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050675">
        <property type="entry name" value="OAF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069:SF32">
        <property type="entry name" value="ARGININE METABOLISM REGULATION PROTEIN II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069">
        <property type="entry name" value="OLEATE-ACTIVATED TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="8">
        <location>
            <begin position="22"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="140"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="235"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="142"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="169"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="235"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR23761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="833" mass="94649" checksum="3CBF1DBBE682BE73" modified="2009-07-28" version="1">MKRQSSAGGKAKEVRRVKTFTGCWTCRSRKVKCDLGKPTCQRCDKSGLECGGYDIKLRWSQPIRFDNFGQVDPNTSSSACADEPQSQRRNVGFVTYKDEYEFYEDMDDELSELHSPSLDKIADNKTWIIKKFGVFRGTDKVKTKHVPRRKKRRKQDQQSRSPKIRSEVSGVTSSNMGDDTELHSAQNIANLFDFDLSMNFNGNEWIANELRDDALLSASAIQGIPMDSLFDRPKNFSTSLPATSESSNTSAPRSEGPSVQESQDKQRHNSQQTGSVNGADFSQIYKLLFHRGPDLLRTHRSNSVPTDHQSTPRTIVPLQNIILVDSSGPEGHMPKEVLEVVESEVPEPRIFNVPNNSNPLLTLPTTGLKVHGLTRFLLNYYLQNVVDLMTVVALPTNPWRTIYFPRASRALGDLAGVGYTSNSRNSLLNAILAVSCFNLQSKFPRNSPEMRYYVQLGIELRGQASDFLKICLESTAKQERYKDILTAILSMNSIDVVWGTMADCQYHLMLCENLVEERMKTRPKISGKARSLHRIFSFLKLIQDSTALDKVQEKEIVLGKSQSENLEEELNRGDQEEMGEFKESLDEVNGKIRIEFVKHGGGSGSTPIFTNIASQTYYYPSSTAANNDFLSTDALYGLPNSIILLFSDCVRLARHREYYRMRKLDIPSSYYVLCTTFEKKLMGWKSEWNFFKPDTRDFLNDTVEGVYHHTISFYEGLIIYHHTMVRGQGHKGVQNNVGKVLDHLDKLAALIHDKGVKIVPLIWQGFIAGCAAIDTGLQLKFKEWAAKLANSGMGSYWGARQIMFEVWRRRMNGEHCDNWFDVYRDWEMNLMLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJ01</accession>
    <name>C5DJ01_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0E16522p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0E16522g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554199.1">
        <property type="nucleotide sequence ID" value="XM_002554153.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJ01"/>
    <dbReference type="FunCoup" id="C5DJ01">
        <property type="interactions" value="302"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJ01"/>
    <dbReference type="GeneID" id="8292371"/>
    <dbReference type="KEGG" id="lth:KLTH0E16522g"/>
    <dbReference type="eggNOG" id="KOG2742">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023194_3_1_1"/>
    <dbReference type="InParanoid" id="C5DJ01"/>
    <dbReference type="OMA" id="NEMQSPE"/>
    <dbReference type="OrthoDB" id="64915at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006740">
        <property type="term" value="P:NADPH regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004104">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42840:SF5">
        <property type="entry name" value="NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42840">
        <property type="entry name" value="NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02894">
        <property type="entry name" value="GFO_IDH_MocA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase N-terminal" evidence="1">
        <location>
            <begin position="5"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase C-terminal" evidence="2">
        <location>
            <begin position="140"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR23762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="350" mass="37977" checksum="62C2DFC09BC666DE" modified="2009-07-28" version="1">MDSVLNVGIVGTGIFARDSHLPAYQQLPNEFKVVAAFNRTKSKAEAFAEDAGIEKEKVFDNLDDMLKDESISAIDALLPAQFNAETVEKCIAARKPVILEKPIAATMAQARKIVEMSDATTLPIGIAENWLFMNAASALKEQLAEIGPITAFTYNSTGPFVKENKYLSTSWRQNPEHIGGFLSDGGVHQLALLTEVLGEVESVSALTKQVRKQSGTDDVVFSTVKLRDSDVIGTFTYGSAFGSCKKSVYFKVYGLNGAATLDLSDKKKPVIKVTVGDSAEANAKENTIEIDEHPSGGIQEEFLNFRESILKKEKRVIRGSPRVAFHHLACVAAFLDSSAKDGDSVVVEQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJ13</accession>
    <name>C5DJ13_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0E16808p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0E16808g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554211.1">
        <property type="nucleotide sequence ID" value="XM_002554165.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJ13"/>
    <dbReference type="FunCoup" id="C5DJ13">
        <property type="interactions" value="468"/>
    </dbReference>
    <dbReference type="GeneID" id="8292384"/>
    <dbReference type="KEGG" id="lth:KLTH0E16808g"/>
    <dbReference type="eggNOG" id="KOG2504">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_1_2_1"/>
    <dbReference type="InParanoid" id="C5DJ13"/>
    <dbReference type="OMA" id="AVNVICI"/>
    <dbReference type="OrthoDB" id="2213137at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17352">
        <property type="entry name" value="MFS_MCT_SLC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF315">
        <property type="entry name" value="TRANSPORTER MCH2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="106"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="149"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="180"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="209"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="268"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="326"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="386"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="411"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="480"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="64"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="72"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR23774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="566" mass="62406" checksum="6BFC24303EBE4BE2" modified="2009-07-28" version="1">MNLSAHSSNEATVGLTAVPQPEEMERDEVRRSTEVTDEGGSSFKHKFESGSNMLQRFKREIFDDGTKEQSSTDDSSQQSSASLQTVNENLTEGSLEAPDNPPDGGYGWVCCLCVTLIMFSTWGANSGFGVFLAYYFNNGVFEGASKYDYALVAGMTVAFGQGLAPFAMFLTRILGCKPPMYTGVVMLFCGFLLASFAKKLWQLYLTQGVLVGVSIALLYAPATTVIPGWFLKKRSLAIGMSLIGTGAGGVTYSVSVNKLIQDSGDQTWALRMMAISCTITCIVATVLIKPRNPPKPTYFNSWSEVIREFKLIFSTRVARIYSVDLVAVWFVFALFGYNLMVFTLSPYAVAKGLSAHQASILTTCLNAAQTVGRPIMGYLGDRFGRINTTALLTTVLSTFMFAFWIPCHNFIQLLMFSICIGSCVGVGNVMSTVLVADVVSPADFLPAWGLVNSLGAPFLLECEIIAQALTDTKNPSNPYLHTQIFAGLCFVCALVLMLVYRELRVRNCLQEQLESLPNPRMQYSTDQEQQKNYKNSASEKEYFECAEILTANPINFFRRMFYPTKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJ89</accession>
    <name>C5DJ89_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0F14454p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0F14454g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554815.1">
        <property type="nucleotide sequence ID" value="XM_002554769.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJ89"/>
    <dbReference type="FunCoup" id="C5DJ89">
        <property type="interactions" value="84"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJ89"/>
    <dbReference type="GeneID" id="8293043"/>
    <dbReference type="KEGG" id="lth:KLTH0F14454g"/>
    <dbReference type="eggNOG" id="ENOG502RYF8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048121_1_0_1"/>
    <dbReference type="InParanoid" id="C5DJ89"/>
    <dbReference type="OMA" id="NWRDKGT"/>
    <dbReference type="OrthoDB" id="272512at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="2">
        <location>
            <begin position="89"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR24378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="310" mass="34444" checksum="67D7B5240BAB7EDF" modified="2009-07-28" version="1">MEKFTNWRDKGTGIAPFIPTPPPLAQEKGLKGFLGGISFVLKLVLASPIVLVALLLKWTPAYHPLCKVAIKMIFGWNLQVSVQGVKSRKQGPQFMPSKGKVYIVNYTSPLDPLVLWFIARGPVAFCVPKPQRKTASLYRLNFWEIVQFTLGGSTWGSAQHDFQEIKSAMELSNYVTYVFAEGTTSNGKSVLPFVVTQQFWNEFLGEPAVGSSSSVKTLTSTASRDAEVRAIHIKINSSLTTPLRIGAWRYLARASSQGVTYKCRISEPLGRDLEKTRVALCGGDKFKLVGKELNTESKMKFAVEYGSRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJD3</accession>
    <name>C5DJD3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">KLTH0F15510p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0F15510g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="9">Binds 1 Mg(2+) per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the TPP enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554859.1">
        <property type="nucleotide sequence ID" value="XM_002554813.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJD3"/>
    <dbReference type="FunCoup" id="C5DJD3">
        <property type="interactions" value="67"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJD3"/>
    <dbReference type="GeneID" id="8293090"/>
    <dbReference type="KEGG" id="lth:KLTH0F15510g"/>
    <dbReference type="eggNOG" id="KOG1184">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013748_0_2_1"/>
    <dbReference type="InParanoid" id="C5DJD3"/>
    <dbReference type="OMA" id="AQEISVM"/>
    <dbReference type="OrthoDB" id="308383at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004737">
        <property type="term" value="F:pyruvate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000949">
        <property type="term" value="P:aromatic amino acid family catabolic process to alcohol via Ehrlich pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02005">
        <property type="entry name" value="TPP_PDC_IPDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07038">
        <property type="entry name" value="TPP_PYR_PDC_IPDC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000019">
        <property type="entry name" value="Pyruvate decarboxylase isozyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000024">
        <property type="entry name" value="Pyruvate decarboxylase isozyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012110">
        <property type="entry name" value="PDC/IPDC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047214">
        <property type="entry name" value="TPP_PDC_IPDC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047213">
        <property type="entry name" value="TPP_PYR_PDC_IPDC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43452">
        <property type="entry name" value="PYRUVATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43452:SF3">
        <property type="entry name" value="TRANSAMINATED AMINO ACID DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036565">
        <property type="entry name" value="Pyruvt_ip_decrb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme N-terminal TPP-binding" evidence="13">
        <location>
            <begin position="24"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme central" evidence="11">
        <location>
            <begin position="246"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="12">
        <location>
            <begin position="436"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <label>1</label>
            <note>substrate; ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <label>1</label>
            <note>substrate; ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <label>2</label>
            <note>allosteric activator</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="506"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="533"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="535"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="539"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <label>1</label>
            <note>substrate; ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR036565-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR036565-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR24422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="625" mass="70177" checksum="B73632793120E027" modified="2009-07-28" version="1">MSPIGILGNQRSSAVANERMRIPLGQYIFERIVSSGTRTIFGVPGDFNLSLLEHIYNPEVALKGIKWIGTCNELNAAYAADGYSRYTNKIGCLITTLGVGELSALNGIAGAFAEDVKILHIVGVAPSQFYEGSAQSSHNVHHLVPAVFDSNIHAPNHRVYRDMVDKRVSCSSLFLDDISSACDQIDTVISDIYRYSKPGYIFIPADFTDKLVDARNLEKRPAILLRDVVSGFCINDHKCQELAEYILESMYSSSRPAIIADILCDRYDLTSSLRELVNKTHVWNFASSMGKSILDENNLGFRGVYNGSESQDQVLKDFQTCDFLLHVGVVKNEMNTGHYSYKFSNHSNIVELHPSYVNFIDADTGKNRYVTGQYFFKVLDMMTQMIDPTKLHLGYQESIPSRTQEYCGPPHSSVTHSYLQTHFPKFLSSGDVFVVDTGSFQFGVRDYRFPSDLKYISQSFYLSIGMALPCALGVGLGMQDYPSNHIISSALVQRNYQPRLILCEGDGAAQMTIQELTSFLRYKVPIDLFIWNNGGYTVERAIKGPNRPYNDIMAWEWTELLRVFGDPKLENSVSQKVSSADQMSELLKTFRSSDKRSKLVRLVEVMLGVMDFPEQLKAMAIACRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJI1</accession>
    <name>C5DJI1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0F16610p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0F16610g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldo/keto reductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554907.1">
        <property type="nucleotide sequence ID" value="XM_002554861.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJI1"/>
    <dbReference type="FunCoup" id="C5DJI1">
        <property type="interactions" value="445"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJI1"/>
    <dbReference type="GeneID" id="8293140"/>
    <dbReference type="KEGG" id="lth:KLTH0F16610g"/>
    <dbReference type="eggNOG" id="KOG1577">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJI1"/>
    <dbReference type="OMA" id="VHWPSEG"/>
    <dbReference type="OrthoDB" id="416253at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000007">
        <property type="entry name" value="NAD(P)H-dependent D-xylose reductase xyl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11732">
        <property type="entry name" value="ALDO/KETO REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00798">
        <property type="entry name" value="ALDOKETO_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="6">
        <location>
            <begin position="28"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="3">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="5">
        <location>
            <position position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR24470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="329" mass="37462" checksum="036C184E5152F7BB" modified="2009-07-28" version="1">MVYVSETVKLNNGLEMPLVGLGCWKIPNEVCASQVYEAIKLGYRLFDGAQDYGNEKEVGQGIRKAIDEGIVKREDLFVVSKLWNSYHDPKNVKKALKRTLQDLGLEYLDLFYIHFPLAFKFVPFEERYPPGFYTGAEDEKKGVISLEKVTVLDTYRALEQCVDEGLIKAIGVSNFRGILLHDLLAGAKIPPACLQIEHHPYLTQEKLVEYCKQQGIQVVAYSSFGPQSFLEMDSQLAKSTPTLFEHDVVKKVANNHGVSTSEVLLRWATQRRVAIIPKSSKKERLLNNLQINEKLTLSDEELQEISSLNKDIRFNDPFNWLDMKFPVFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJN8</accession>
    <name>C5DJN8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0F17930p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0F17930g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554964.1">
        <property type="nucleotide sequence ID" value="XM_002554918.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJN8"/>
    <dbReference type="GeneID" id="8293199"/>
    <dbReference type="KEGG" id="lth:KLTH0F17930g"/>
    <dbReference type="eggNOG" id="ENOG502SD7R">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119618_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJN8"/>
    <dbReference type="OMA" id="FRIREEY"/>
    <dbReference type="OrthoDB" id="3970753at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR24527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="156" mass="18236" checksum="E9850D69D53114E6" modified="2009-07-28" version="1">MCPGVLVCRKQLFGTASLHNIVPSELSHGWEPQVELFEGLICVCELMSKNDDIPWYRIVFEWQDGGGEKRQGQVKFFGQTAIMKGTIDLNKTVKNREEWFEILMECSDQRSVALELRIKDIREDQNFRDLLFRIREEYEMIDEMMGESDDFGDFIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DK70</accession>
    <name>C5DK70_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">SUN-like protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0F02244g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="9">Interacts with EMP65.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the SLP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554308.1">
        <property type="nucleotide sequence ID" value="XM_002554262.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DK70"/>
    <dbReference type="FunCoup" id="C5DK70">
        <property type="interactions" value="54"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DK70"/>
    <dbReference type="GeneID" id="8292500"/>
    <dbReference type="KEGG" id="lth:KLTH0F02244g"/>
    <dbReference type="eggNOG" id="KOG1396">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006633_2_0_1"/>
    <dbReference type="InParanoid" id="C5DK70"/>
    <dbReference type="OMA" id="YVIIELC"/>
    <dbReference type="OrthoDB" id="266334at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000099">
        <property type="entry name" value="Uncharacterized protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045120">
        <property type="entry name" value="Suco/Slp1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012919">
        <property type="entry name" value="SUN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12953">
        <property type="entry name" value="MEMBRANE PROTEIN CH1 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12953:SF0">
        <property type="entry name" value="SUN DOMAIN-CONTAINING OSSIFICATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07738">
        <property type="entry name" value="Sad1_UNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51469">
        <property type="entry name" value="SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0732">Signal</keyword>
    <keyword evidence="2 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950513" description="SUN-like protein 1" evidence="13">
        <location>
            <begin position="20"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="572"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="domain" description="SUN" evidence="14">
        <location>
            <begin position="181"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="26"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="26"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="68"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00064635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00075366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR23871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="701" mass="78792" checksum="2D1ED9B00A5386E8" modified="2009-07-28" version="1">MVVIGRILHLLVLFRASACFTSEPQEISTSSSAGQSELSSFLSKESRGTSTTQQSSAEVPQTQYDQHSVSTVSVTSSGNFTQTSSSLDKDTYIENSAYSPPDESGSASRSLQGTKSSLVAIAEAQESDFNETDTTFLSFDEWKKEKLGEESLQKAKPPARVNRPVDSSVYKGEAMGDDFEIDVGLFTSSKQDLNEEPEGKLYKDKFNYASLDCAATIVKTNSEASGANAVLHENKDKYLLTPCSASNKFVVIELCQDILVEEIVMANYEFFSSTFSKVRFSVSNSYPPKNGWKVLGEFDAANTRNLQKFGISNPLIWARYLRVEVLAHHGNEFYCPISVIRVHGKTMMDDFKLDESNSLYSEDAVEQASPEGQLKECRQEKLAPHNLSESMLRECQFPQFPQADNVSILSKLDFLSTQCPAVLPHLKFDQFLKDINQSVCDTKIHQPQLDISTSAPSSSTEESIFKTIMKRLTLLESNSTLSLRYIEEQSMLLSKAFASLERNQAKKFQSLVQAFNQTIVSNLGDINFFTQQLRESSIKLLEEQKLANDQFTSETFHRLESMKKDAIFQRRLSYTMLFAFVILLVYVLLTKEAYIDEYMEDDGWYLNSPPLKKFKDNFLRRAGKSTDDRRSLVFCTSRDYSENDEKSDVSASTSSASLYDEFATKVAHEMDGNVLSYRKPTTSLEEEDIDIDEALSEGSSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKF9</accession>
    <name>C5DKF9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0F04356p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0F04356g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554397.1">
        <property type="nucleotide sequence ID" value="XM_002554351.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKF9"/>
    <dbReference type="FunCoup" id="C5DKF9">
        <property type="interactions" value="1159"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKF9"/>
    <dbReference type="GeneID" id="8292592"/>
    <dbReference type="KEGG" id="lth:KLTH0F04356g"/>
    <dbReference type="eggNOG" id="KOG1279">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004447_2_1_1"/>
    <dbReference type="InParanoid" id="C5DKF9"/>
    <dbReference type="OMA" id="YASHYPI"/>
    <dbReference type="OrthoDB" id="118550at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016514">
        <property type="term" value="C:SWI/SNF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000020">
        <property type="entry name" value="SWI/SNF complex subunit SMARCC2 isoform c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000014">
        <property type="entry name" value="SWI/SNF complex subunit SMARCC2 isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032451">
        <property type="entry name" value="SMARCC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007526">
        <property type="entry name" value="SWIRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12802:SF41">
        <property type="entry name" value="BRAHMA ASSOCIATED PROTEIN 155 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12802">
        <property type="entry name" value="SWI/SNF COMPLEX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04433">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16495">
        <property type="entry name" value="SWIRM-assoc_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50934">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="SWIRM" evidence="7">
        <location>
            <begin position="374"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="6">
        <location>
            <begin position="569"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="8">
        <location>
            <begin position="575"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="211"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="331"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="520"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="800"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="14"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="79"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="160"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="211"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="237"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="344"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="802"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="821"/>
            <end position="830"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR23960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="851" mass="93997" checksum="FDEC719FD170D3BB" modified="2009-07-28" version="1">MSVEQTNSPYAHSLQHESATESGDAHDAALFGDADEDATQQGPQGDDITGTADKGAEENPEAVLMSPTAADVLSQSRGLEAESRETAAHEPHQELEESAAPVEQSDMGGPVEASDHAEAGKQQEPSLVPDVSEQLGLPEQQDIEKQQGSDVQQETAALPASSAQAESNEASASVRGNESIEQLGHGEELKLGNGAEKFGLSGAPDIALQTTHQTQDQVPQTEQSFPAENPVQAEPLQAEQRVQVEQRVQSEQPVQLEQPVKMKQPVEMDQPVEMDQPVEMDHPVQMQQPVQAEQPVPAKQPIQAEQLAQAAQPVQTEQPVKQENAIKEETSLQHEQQLHAESSPLPQNNGTAAQLQEMGPEAPSRLVVPQSHEIVIPSYARWFHLQKIHSIEKQSLPEFFTNRIPSKTPQVYVKYRNFMVNSYRLNPNEYFTATAARRNLCGDAGAILRLHKFLTKWGLINYQVDAKAKPKQVEPPFTGEYATKHDAPRGLFPFESYKPAIQIPDLSRLKKLMHQIDPPASSTKSALAAVSPSPGDSSDKKRSLEDGDETPVKPKLQRPNVLEMVDRDWDEESVKKLLEGIQKFQTDWLRVAQYVGNKTPEQCILRFLQLPIEDAYLQSQSGEELGPLKYAPHLPFSKADNPVMSTIAFLAGLVDPKTVKKMTSRAIDSVKQERETLSAEGDNYVKEGSEIALASIGLRSHVFATNEERQMTALAHDMVQTQMKKINLKLKLLGTMEKSLEMEKKALQKQQEDVFIQRLSLAKHSQTLTAKIQQCLDDHDDKEKLQAHLNAIKEMQAHPARISIGKSQSSSKSAVNGSSQEQAFSSTSDLNEPFVKPVSVDAPQSYRYWSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKL6</accession>
    <name>C5DKL6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dynein heavy chain, cytoplasmic</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Dynein heavy chain, cytosolic</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ordered locus">KLTH0F05720g</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Cytoplasmic dynein acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. Required to maintain uniform nuclear distribution in hyphae. May play an important role in the proper orientation of the mitotic spindle into the budding daughter cell yeast. Probably required for normal progression of the cell cycle.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Consists of at least two heavy chains and a number of intermediate and light chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein heavy chain family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554454.1">
        <property type="nucleotide sequence ID" value="XM_002554408.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DKL6">
        <property type="interactions" value="787"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKL6"/>
    <dbReference type="GeneID" id="8292652"/>
    <dbReference type="KEGG" id="lth:KLTH0F05720g"/>
    <dbReference type="eggNOG" id="KOG3595">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000038_7_0_1"/>
    <dbReference type="InParanoid" id="C5DKL6"/>
    <dbReference type="OMA" id="NERQMTR"/>
    <dbReference type="OrthoDB" id="447173at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000235">
        <property type="term" value="C:astral microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005816">
        <property type="term" value="C:spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008569">
        <property type="term" value="F:minus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000741">
        <property type="term" value="P:karyogamy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902850">
        <property type="term" value="P:microtubule cytoskeleton organization involved in mitosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000070">
        <property type="term" value="P:mitotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030473">
        <property type="term" value="P:nuclear migration along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000996">
        <property type="entry name" value="Cytoplasmic dynein heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002357">
        <property type="entry name" value="Glutathione S-transferase class-mu 26 kDa isozyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.100">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.180.20">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.720">
        <property type="entry name" value="Region D6 of dynein motor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035699">
        <property type="entry name" value="AAA_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035706">
        <property type="entry name" value="AAA_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041658">
        <property type="entry name" value="AAA_lid_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042219">
        <property type="entry name" value="AAA_lid_11_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054354">
        <property type="entry name" value="DYNC2H1-like_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042222">
        <property type="entry name" value="Dynein_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043157">
        <property type="entry name" value="Dynein_AAA1S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041466">
        <property type="entry name" value="Dynein_AAA5_ext"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024743">
        <property type="entry name" value="Dynein_HC_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024317">
        <property type="entry name" value="Dynein_heavy_chain_D4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004273">
        <property type="entry name" value="Dynein_heavy_D6_P-loop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013602">
        <property type="entry name" value="Dynein_heavy_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013594">
        <property type="entry name" value="Dynein_heavy_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042228">
        <property type="entry name" value="Dynein_linker_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45703">
        <property type="entry name" value="DYNEIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45703:SF36">
        <property type="entry name" value="DYNEIN HEAVY CHAIN, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12774">
        <property type="entry name" value="AAA_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12775">
        <property type="entry name" value="AAA_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12780">
        <property type="entry name" value="AAA_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12781">
        <property type="entry name" value="AAA_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18198">
        <property type="entry name" value="AAA_lid_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08385">
        <property type="entry name" value="DHC_N1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08393">
        <property type="entry name" value="DHC_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22597">
        <property type="entry name" value="DYN_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17852">
        <property type="entry name" value="Dynein_AAA_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03028">
        <property type="entry name" value="Dynein_heavy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12777">
        <property type="entry name" value="MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12 17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-0243">Dynein</keyword>
    <keyword evidence="5" id="KW-0415">Karyogamy</keyword>
    <keyword evidence="7" id="KW-0493">Microtubule</keyword>
    <keyword evidence="13" id="KW-0505">Motor protein</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="18">
        <location>
            <begin position="1799"/>
            <end position="1933"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="18">
        <location>
            <begin position="2075"/>
            <end position="2209"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="18">
        <location>
            <begin position="2423"/>
            <end position="2573"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="3033"/>
            <end position="3060"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00033439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00053342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAR24017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="4108" mass="468253" checksum="CD969E098C637A38" modified="2009-07-28" version="1">[encoded data, 4108 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKP7</accession>
    <name>C5DKP7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0F06468p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0F06468g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554485.1">
        <property type="nucleotide sequence ID" value="XM_002554439.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKP7"/>
    <dbReference type="FunCoup" id="C5DKP7">
        <property type="interactions" value="416"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKP7"/>
    <dbReference type="GeneID" id="8292686"/>
    <dbReference type="KEGG" id="lth:KLTH0F06468g"/>
    <dbReference type="eggNOG" id="KOG0596">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019372_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKP7"/>
    <dbReference type="OMA" id="YRETQIG"/>
    <dbReference type="OrthoDB" id="20524at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004712">
        <property type="term" value="F:protein serine/threonine/tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033316">
        <property type="term" value="P:meiotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098813">
        <property type="term" value="P:nuclear chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034501">
        <property type="term" value="P:protein localization to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14131">
        <property type="entry name" value="PKc_Mps1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000377">
        <property type="entry name" value="Checkpoint protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000131">
        <property type="entry name" value="Dual specificity protein kinase TTK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027084">
        <property type="entry name" value="Mps1_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22974:SF21">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN KINASE TTK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22974">
        <property type="entry name" value="MIXED LINEAGE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="514"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="243"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="340"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="67"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="97"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="121"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="146"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="175"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="197"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="213"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="363"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="543"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR24048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="837" mass="94678" checksum="CC315835F589DF2A" modified="2009-07-28" version="1">MSSYTVRSSVPGAPSNRRLEFPARRTSVQSLARDDSDDENMLGPPKLSNFGSALLSDKENVEPYPYSRNQSVSNAKRYSQPPRAAARPETFGAVREFSSSNTNNSVAGPYSTFHSEEHSDSTMFATSKHNQQSMTTFNPETPSEQGKPEDMYLEKSRSRVKSMQQTLRDELSLRSSKRRNRRFVSSRFNLLGPAKRASSNTGTPNHSELADESNVSQQEANFSHSSSAGATAHQNEYNVFYESQNSSSPQKDSAAPTDHTSINYDNIEFGDLNPYQYLKKHNIPTTELPNISRLYFERQKGENRKTALRKNIAAKDAFQTRLTSASLSNSPLLKHVDLIKREQSIPKRQPRRKISDHATNNDSKAWSSPVSSSKGLKTAPSRDGTSPRASEMYENIERPERDTFGSIRRREALSNININSNYHQLQNKRTKYSTENLEEARGIHRPPFEHSVQEERFANHGFKHRSPPAVPERKHVEIVEPLKTTESIPLKPPATSGDSYNRRKPTIYVNGTEYEKLELLGRGGSSKVYKVKNVANNKVYALKRVSFDEFDDSSIEGFKGEIELLKKLETKPRVVKLIDHEMDHGVLYEVMECGDHDLSHTLALRSGMGLDVEFVRFHSQEMLKCVKVVHDSGIVHSDLKPANFVFVKGILKIIDFGIANAVPEHTVNIYRETQIGTPNYMAPEALVAMNYTQGQPAEQNRWKVGKPSDIWSCGCIIYQMIYGRPPYGGFQGQNRLLAIMNPEVKIVYPEKTSNGDVVPKTAIDTMKACLERNPEKRWSVDEVLNGPFVKPIAVTHFFIRDLIKNSVKYGANQREISNEKIEELAEDVWHRLADFRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKR1</accession>
    <name>C5DKR1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0F06776p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0F06776g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554499.1">
        <property type="nucleotide sequence ID" value="XM_002554453.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKR1"/>
    <dbReference type="FunCoup" id="C5DKR1">
        <property type="interactions" value="111"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKR1"/>
    <dbReference type="GeneID" id="8292700"/>
    <dbReference type="KEGG" id="lth:KLTH0F06776g"/>
    <dbReference type="eggNOG" id="KOG0274">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_425266_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKR1"/>
    <dbReference type="OMA" id="CTTPQGC"/>
    <dbReference type="OrthoDB" id="2095648at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052301">
        <property type="entry name" value="SCF_F-box/WD-repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14381">
        <property type="entry name" value="DACTYLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14381:SF1">
        <property type="entry name" value="F-BOX_WD REPEAT-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00726">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="8"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="571"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="598"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="617"/>
            <end position="626"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR24062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="650" mass="73672" checksum="69396945765944DE" modified="2009-07-28" version="1">MIDAVGSGTSLQSLPTEILINILSHLDEKDLYAVQATCKHFRDIINDEELWKNLFVSRINTRLFPSFSRSLKFSVEYVERNRGLNEWRHNRAIKTRYTVATQNQYNQLEQMVFDYPRCACYSDGVIMLVQLHSRRRKDRLTYMPCTTPHGCSTMHFNINAAVFGRFDGRVFGKLLTNKSHLCPVTEFNAMHRSCVTAITTAALEDSSEDWCVSGCESGQVIWWREAKLQKSLQISNKPILRLALHKDWTVAVDSEHICIVQKMDTILRLDIPSNLRRSPNQIQFFKVDFGGKLLVLGDTTEIYVISIDVDQDFGHTRSLRFENSVQNVFIDEATAKRTQDASIAGGDGCFMGILTADNSVHTVNIRTPGSNLSIQTKLTFIDEVHVAQINNLVLVCAFSGSLGIFDAANGSELRVVRKTEKTPQFLGVSHGRMIVGSGNVLHFLQYAGLEDKQKKTGSSQVTRGNKWNEVLHSQLDLYNEDENSRLQEQKRLEKLRRKFLGDVDDEETQLRIALMESEAAAANFPFSAMKESSTEPDEELLRAIEESKLSFENSLASDEQGEDLELLRAIQQSREEEESRRTSRRSQRRMAPLGELVGSSSIEPQSNRQEQRANPVCLQSQASEVSPDQHRHFEDEELELAKALSLSELN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>C5DL35</accession>
    <name>C5DL35_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0F09636p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0F09636g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554623.1">
        <property type="nucleotide sequence ID" value="XM_002554577.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DL35"/>
    <dbReference type="FunCoup" id="C5DL35">
        <property type="interactions" value="1000"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DL35"/>
    <dbReference type="GeneID" id="8292830"/>
    <dbReference type="KEGG" id="lth:KLTH0F09636g"/>
    <dbReference type="eggNOG" id="KOG0486">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028430_1_0_1"/>
    <dbReference type="InParanoid" id="C5DL35"/>
    <dbReference type="OMA" id="CELKLNV"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051306">
        <property type="entry name" value="Homeobox_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46123">
        <property type="entry name" value="MIX-TYPE HOMEOBOX GENE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46123:SF4">
        <property type="entry name" value="MIX-TYPE HOMEOBOX GENE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 5 6" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4 5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="8">
        <location>
            <begin position="64"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="5">
        <location>
            <begin position="66"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="420"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="13"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR24186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="484" mass="53789" checksum="382A159F9ADB3441" modified="2009-07-28" version="1">MEEYQANGIEFNPQFQPQYEVQNTFENGQSGSEASENAAQTGAQKDGTAGSRAAPAHSDQNRDERPRTKRTRATGEALNILKREFEENPNPNAQNRKRISELTGLPEKNVRIWFQNRRAKHRKTDRIAKPTAVETSVPGNSYLASDFDRIPLDINPNYCFIDVKSLTVGSWKRLKSGNLKADDLSTIKRLSNLSPISINEIMANATDLMVLISKKNHEINYFFSAIANNTKILFRIFFPIATVVDCSLTVAPESLQNEDPATEDAVRAEQDKVCELRLNVSKAPTFAVYFSDGADQFSANQWSICEDFSEERQVSEAYTGGSNIPHIITGLGNSLNFMNSTIQDHNSGNSVLSHTSHSGSQGSVNVSDAQNLILHPEPQSLGPRQQPFFDDFNEHEELYGMGEQNTSQFAHSNANQTHPFTQHAAPAAGNDHGSPQYTESGVPRTPDFLRSHAEMGNEEQSGLNNLLIFEDQNNNNTKNGQSYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>C5DL42</accession>
    <name>C5DL42_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">KLTH0F09790p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0F09790g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554630.1">
        <property type="nucleotide sequence ID" value="XM_002554584.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DL42"/>
    <dbReference type="FunCoup" id="C5DL42">
        <property type="interactions" value="80"/>
    </dbReference>
    <dbReference type="GeneID" id="8292837"/>
    <dbReference type="KEGG" id="lth:KLTH0F09790g"/>
    <dbReference type="eggNOG" id="KOG0770">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015166_3_2_1"/>
    <dbReference type="InParanoid" id="C5DL42"/>
    <dbReference type="OMA" id="MMAPINT"/>
    <dbReference type="OrthoDB" id="415315at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000095">
        <property type="entry name" value="Mitochondrial carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45667">
        <property type="entry name" value="S-ADENOSYLMETHIONINE MITOCHONDRIAL CARRIER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="45"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="140"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="247"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR24193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="357" mass="39138" checksum="58ADB0D697192549" modified="2009-07-28" version="1">MISLTSPIPVLHPTHEAHEHRDCVGGNNGANGNGKDKESSSRENSAIWHCVIAGGLGGAIGDSVMHSLDTVKTRQQGAPNAPKYRNMVSAYKTLFVEEGIRRGLYGGYTAAMLGSFPSAAIFFGTYEFTKRKMIDEWGVNDTVSHLTAGLGGDLVSSAAYVPSEVLKTRLQLQGRFNNPYFHSGYNYRNLRDAISVIARIEGWRTLFFGYKATLCRDLPFSAFQFAFYEKFRHWAFSLENKGQDEDLSVLSELTTGAAAGGLAGIITTPMDVIKTRIQTQTPLMALGDSAKLVRIENSLIKGLTAVYRSEGVLGFFSGVGPRFIWTSIQSSIMLLLYQVTLKSLDRLTAPNSAFKHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DL71</accession>
    <name>C5DL71_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Mediator of RNA polymerase II transcription subunit 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Mediator complex subunit 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0F10538g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the Mediator complex subunit 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554659.1">
        <property type="nucleotide sequence ID" value="XM_002554613.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DL71"/>
    <dbReference type="FunCoup" id="C5DL71">
        <property type="interactions" value="219"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DL71"/>
    <dbReference type="GeneID" id="8292871"/>
    <dbReference type="KEGG" id="lth:KLTH0F10538g"/>
    <dbReference type="eggNOG" id="ENOG502QW0Y">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021764_0_0_1"/>
    <dbReference type="InParanoid" id="C5DL71"/>
    <dbReference type="OMA" id="NDKFGIY"/>
    <dbReference type="OrthoDB" id="5310959at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019680">
        <property type="entry name" value="Mediator_Med1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10744">
        <property type="entry name" value="Med1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0010">Activator</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Mediator complex subunit Med1" evidence="9">
        <location>
            <begin position="28"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="332"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="346"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="356"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF10744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR24222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="444" mass="50727" checksum="2A82661C0F011E91" modified="2009-07-28" version="1">MIHAEARQRSQTPHSPPMSDAYAETLGQMICLLQEYKPGLVTLENITRLCKTLKLESFTDNIDNETIRLSTASKIIVIDMDFHKSDKQVKDVKLVLASNFDNFNYYNDGDNGNILFNSLSCFPNLQTFYYNLRFLTTLDTHSNIETEPGIAAKREKLDLFKYFTELPEYLRAYFKDISIDLDIKTNVDNRFGVYLTHNDKAVAQVGIEECQDHDHRFYEFGYRSSGWINESPDNVTTGVILTLQFYDINMHIPKEAIPHDLLLDWDAQRPYTIVNHCRSTPVSNDFTSTLVPATKFNISNENIELLGELLQWAGWWQQILHPVLQIIKCPAPAPGNETKRRSSSNSTTISAQRRSSATRRRRSSQKGRRPSMNESSMVKDGVVPEFSLSEVMSQPVLEDEDPSEPVDLALNEEYIQLGPGSRCDIHMGVHEWQEFLTKLKGCVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLA6</accession>
    <name>C5DLA6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0F11418p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0F11418g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554694.1">
        <property type="nucleotide sequence ID" value="XM_002554648.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLA6"/>
    <dbReference type="FunCoup" id="C5DLA6">
        <property type="interactions" value="218"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLA6"/>
    <dbReference type="GeneID" id="8292907"/>
    <dbReference type="KEGG" id="lth:KLTH0F11418g"/>
    <dbReference type="eggNOG" id="ENOG502S04D">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038624_0_0_1"/>
    <dbReference type="InParanoid" id="C5DLA6"/>
    <dbReference type="OMA" id="GQFSYYK"/>
    <dbReference type="OrthoDB" id="2157866at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="1">
        <location>
            <begin position="217"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="328" mass="38218" checksum="62E5EE53779662F1" modified="2009-07-28" version="1">MDQNEDLKLLYTDTQHFNDVLISSFLFKKSSSSAATNKLASDHKQSAPGTSSPDIGGSHHWFMRGGFHKYWCVLRRGQFSYYKDKNERKPVDVIPADQVLDFRVRMDEYRLDFYTKQKTISFGSESQEVLRGWDAALQEFVSDRRRSSLPMRIYDSGNDENDNECICEDDDDDFEILSEAQPQDPEKERDRNVWSSFKVPDEDREFYAIYNPEQPKRVIQTGTLLCRVKKRLGRKTWKRVNAVLENTSLLIESVSSGRLYKKIDLNGVVDCVEDEKSRYDTGFAVITYDERTKFRAPSEQDTIEWIMNLKSCVLVRKKLEELKSLCEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLM2</accession>
    <name>C5DLM2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Sm protein B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0G01804g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the snRNP SmB/SmN family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555120.1">
        <property type="nucleotide sequence ID" value="XM_002555074.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLM2"/>
    <dbReference type="FunCoup" id="C5DLM2">
        <property type="interactions" value="455"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLM2"/>
    <dbReference type="GeneID" id="8293378"/>
    <dbReference type="KEGG" id="lth:KLTH0G01804g"/>
    <dbReference type="eggNOG" id="KOG3168">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076902_1_2_1"/>
    <dbReference type="InParanoid" id="C5DLM2"/>
    <dbReference type="OMA" id="VRKFQPP"/>
    <dbReference type="OrthoDB" id="2020720at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005687">
        <property type="term" value="C:U4 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070990">
        <property type="term" value="F:snRNP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01717">
        <property type="entry name" value="Sm_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050914">
        <property type="entry name" value="snRNP_SmB/NAA38-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10701:SF0">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN-ASSOCIATED PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10701">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN-ASSOCIATED PROTEIN B AND N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Sm" evidence="12">
        <location>
            <begin position="37"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="127"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="131"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="179"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR24683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="192" mass="21649" checksum="8741B62220A3C5F1" modified="2009-07-28" version="1">MWGFDVYYPHLRIFSQFQMNLNSYRSRGRPEMSVKVGHKSKLADLLRYRIRAVTNDGKAFVGELLAFDAHMNLVLAECVEHRIPSTQVQSLKAKAKEVDAQPKIETRTLGLVVLRGDQVLTTVVESGPTMSKRERAVAHDRKQAQLNKHKRKGKGVVRPGASNNAVAPAPGFQRTSQPQPRPFQPPPGFRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>C5DM84</accession>
    <name>C5DM84_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0G06776p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0G06776g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555332.1">
        <property type="nucleotide sequence ID" value="XM_002555286.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DM84"/>
    <dbReference type="FunCoup" id="C5DM84">
        <property type="interactions" value="191"/>
    </dbReference>
    <dbReference type="GeneID" id="8293601"/>
    <dbReference type="KEGG" id="lth:KLTH0G06776g"/>
    <dbReference type="eggNOG" id="KOG2551">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051938_2_0_1"/>
    <dbReference type="InParanoid" id="C5DM84"/>
    <dbReference type="OMA" id="FEHPGGH"/>
    <dbReference type="OrthoDB" id="2094269at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000279">
        <property type="entry name" value="Family of serine hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005645">
        <property type="entry name" value="FSH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050593">
        <property type="entry name" value="LovG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48070">
        <property type="entry name" value="ESTERASE OVCA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48070:SF9">
        <property type="entry name" value="FAMILY OF SERINE HYDROLASES 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03959">
        <property type="entry name" value="FSH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine hydrolase" evidence="2">
        <location>
            <begin position="3"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR24895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="236" mass="26273" checksum="7FBA7D1224C1D702" modified="2009-07-28" version="1">MSAPKLLFLHGFLQNGKVFSEKSSGIRKLLKKANVQCDYIDGPTTLERKDLPFEMDDEKWQATVDAQLNKSWFFHSDISKELDLTTAIQTVSDHIKANGPYDGIVGFSQGAAVAAIITNRITQLVPDHPPFKVSLIFSGYSFTEPDPEHEGQLRITEKFKQDFTPAPESPTKMLFVYGASDQAVPATRSKYLQEIYASSSPENVKAFEHPGGHMVPNKKDIIRPIVDEICSAVQSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMF6</accession>
    <name>C5DMF6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Actin cytoskeleton-regulatory complex protein END3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Actin cytoskeleton-regulatory complex protein end3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Endocytosis protein 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="ordered locus">KLTH0G08492g</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Actin patch</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the END3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555404.1">
        <property type="nucleotide sequence ID" value="XM_002555358.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMF6"/>
    <dbReference type="FunCoup" id="C5DMF6">
        <property type="interactions" value="147"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMF6"/>
    <dbReference type="GeneID" id="8293678"/>
    <dbReference type="KEGG" id="lth:KLTH0G08492g"/>
    <dbReference type="eggNOG" id="KOG0998">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040829_0_0_1"/>
    <dbReference type="InParanoid" id="C5DMF6"/>
    <dbReference type="OMA" id="DWLIPES"/>
    <dbReference type="OrthoDB" id="1716625at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00052">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000323">
        <property type="entry name" value="Actin cytoskeleton-regulatory complex protein end3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000261">
        <property type="entry name" value="EH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025604">
        <property type="entry name" value="End3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216:SF170">
        <property type="entry name" value="DYNAMIN ASSOCIATED PROTEIN 160, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216">
        <property type="entry name" value="EH DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12763">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12761">
        <property type="entry name" value="End3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00027">
        <property type="entry name" value="EH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50031">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="13" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14 19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="17" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="12" id="KW-0967">Endosome</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EH" evidence="21">
        <location>
            <begin position="8"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="22">
        <location>
            <begin position="40"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="101"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="258"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="311"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="374"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="121"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="258"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="312"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00029684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="CAR24967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="404" mass="44968" checksum="518F32786AA8B14E" modified="2009-07-28" version="1">MPKLEQFEIKKYWQIFLGLKPQENKVTHDQVLPILYNSKLDSSILNKIWFLADIDDDDNLDFEEFVICMRLIFDMVNKNIDTVPDDLPDWLIPGSKAKLDAQRRKKESAEAQSAVPEASKPAPAAPAAPAAPAAPETPETPAVQQKAPELDWYISPANKELYASTYDACDTFTDGTVSYPALAGVVRSVFPNVSSSEVDRMWLLVNPQKSPSINRDPAFYLIHALKQRNDMGCELPSELPHAIKEICNQHRVSYDLNSSQADVKRSSSPTKPADYHASASVRERSSNFRSGGGAEGEVGQIKKQIADLDAQPSGTPQVVSNPSDVPSDPNTARKQFEELLRYREEQLRYSGNTHNGSINVKGIKEDLKTIAQQVGVLEQFLANKKQELQQLQSESRNLNYSGRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMI6</accession>
    <name>C5DMI6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0G09240p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0G09240g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555434.1">
        <property type="nucleotide sequence ID" value="XM_002555388.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMI6"/>
    <dbReference type="GeneID" id="8293711"/>
    <dbReference type="KEGG" id="lth:KLTH0G09240g"/>
    <dbReference type="eggNOG" id="ENOG502S78Y">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_533242_0_0_1"/>
    <dbReference type="InParanoid" id="C5DMI6"/>
    <dbReference type="OrthoDB" id="4031801at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="173"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="478" mass="54811" checksum="FC0D53DFDB7E1823" modified="2009-07-28" version="1">MPALFAEAQHLTRNDVLQSIFENNSDQEDALPPTPGYLFFLEDHEDRKTPELYLDLAQQDPVLSDVSFHGEPFLKRFESTMPSNFDSLRWSVDDILADCTPEKSSLWSGAWSDDEALSKDLNSSPFSTPSLTQSNIEDVLARVPEPPIMCFAEFQRHPPQEKPGHIPARASTGKVSKPNNKQASRGKRSSKFNLSAKRALLQKLISRMQMRTRLEQTYEHGILVRRNVAKELAIEGEGATPQLQFCGTRFQDVQTFLQSQATLHQDACHFRTIWSYNREQKGSTIILTLSLTDEQSKCGENEYQFSVISRYLPYDCFGRPHGAVKKKLNRVQEELEREKETRKGDTYCWRHFLTSFEYFKLVSFMLGKEYDFNLNDNQTGETRGSDGKVISPEKRHDMRTRSHAKIYFEGKAVKSAHELLNDETISSHKSFIVGTFGQIMAYTNKRPFGSDSSLSVMEFKYLEEALEKEINFHVFHGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMK9</accession>
    <name>C5DMK9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-histidine N-methyltransferase</fullName>
            <ecNumber evidence="3">2.1.1.85</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0G09768g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the methyltransferase superfamily. METTL18 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.85" evidence="3"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555457.1">
        <property type="nucleotide sequence ID" value="XM_002555411.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMK9"/>
    <dbReference type="FunCoup" id="C5DMK9">
        <property type="interactions" value="1683"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMK9"/>
    <dbReference type="GeneID" id="8293735"/>
    <dbReference type="KEGG" id="lth:KLTH0G09768g"/>
    <dbReference type="eggNOG" id="KOG2920">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038704_1_1_1"/>
    <dbReference type="InParanoid" id="C5DMK9"/>
    <dbReference type="OMA" id="NLLLTWH"/>
    <dbReference type="OrthoDB" id="1723750at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018064">
        <property type="term" value="F:protein-L-histidine N-tele-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614:SF39">
        <property type="entry name" value="HISTIDINE PROTEIN METHYLTRANSFERASE 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="14"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="21"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR25020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="375" mass="41871" checksum="BEDE6B33AE493951" modified="2009-07-28" version="1">MSFQFALSSADLSDDELSDSQNSHQISTGDITGAATPAVNPLDAPHLRSNELKQPQWEGLNKILESLKDVRVSFEKIHTPDKGIPLYRRELFDVKHQLMTESDEKDDEQSKVELEILMGETNEDVRKNVYEGGLKSWECSIDLVDALSTDFGNGFSSGTILELGCGTSLPSEYLFAQLLKSNRRCDAKLILADYNDSVLRLVSIPNLIITWALLTLDEQKLTTLQQGGNETVPIVEYELIFTSELLTAFEEDIVKRGISILLASGTWGRDLCSLLSPELSRSEEVLLLSSETIYHPETLPIISELILELMQLTAHASNSTIKTLVAAKDIYFGVGGSVIEFERYMQQRIKERRLGLKLFTQKVQAGLKRSIVSIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMX4</accession>
    <name>C5DMX4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">KLTH0G12408p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0G12408g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555572.1">
        <property type="nucleotide sequence ID" value="XM_002555526.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMX4"/>
    <dbReference type="FunCoup" id="C5DMX4">
        <property type="interactions" value="230"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMX4"/>
    <dbReference type="GeneID" id="8293854"/>
    <dbReference type="KEGG" id="lth:KLTH0G12408g"/>
    <dbReference type="eggNOG" id="KOG0802">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020039_0_0_1"/>
    <dbReference type="InParanoid" id="C5DMX4"/>
    <dbReference type="OMA" id="HIFGREC"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46539">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE ATL42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46539:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE ATL42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="229"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="140"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="287"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="407"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="467"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="552"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="23"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="195"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="299"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="363"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="374"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="409"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="490"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="566"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="583"/>
            <end position="595"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR25135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="603" mass="65504" checksum="55FE283F925CC332" modified="2009-07-28" version="1">MNSNNDTSSSGSGGGEEPDRTGTQEPGTRPTRNIMVSIQYAFVSGLRLGANGGNPEEGTGDGATMPPIGDFVLNFTDVPGTATREQLDEVVALASGVAVNRMNRRFNRSRGISKTAFENLPVLKFSAVPQESCSICYDDFEDEAPEPQPENEQSRKRSRDHKADDGLHSSSSSKKHRSERDSGQSSEDDEETGGEEPTPAPSTSEPPKENEADTNNQESIAYKHSPLKLPCGHIFGRECIRQWTNEHNTCPICRARIVGADGLEDHSVNDDLEDQISLERIRRLLYDTSPEAPERPQPTNEDNNNSTSTTTSTTTNDAANDTTNNTTTNPSAGSQVPGVSANGNPPALGGAFHNFVVLRPHQQPATDPANSEQPNPGNLPTNPHLPLLPLLTRQGGFTPIPITFINLRRNPQGQQHTASRNNDGPNDLNDSQMNPSNNTHRDINTHTDNNQENDRLMNIFDHLFSISNNTRPQPEATLRSDATPMPAGTPDSSQTDNQSNSAAPRPIRSHIFNNLFRFARNLRNNSTPQSDHPLSSVSQMFNTGVASFRNQDGVSTMDFSGDMPTPANTHQNQSVPTEERASAPSSESQGRVNDSSDSEGDQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMX5</accession>
    <name>C5DMX5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Peroxin-7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0G12430g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Peroxisome matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the WD repeat peroxin-7 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555573.1">
        <property type="nucleotide sequence ID" value="XM_002555527.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMX5"/>
    <dbReference type="FunCoup" id="C5DMX5">
        <property type="interactions" value="498"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMX5"/>
    <dbReference type="GeneID" id="8293855"/>
    <dbReference type="KEGG" id="lth:KLTH0G12430g"/>
    <dbReference type="eggNOG" id="KOG0277">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046581_1_0_1"/>
    <dbReference type="InParanoid" id="C5DMX5"/>
    <dbReference type="OMA" id="FAVHWNL"/>
    <dbReference type="OrthoDB" id="273771at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005053">
        <property type="term" value="F:peroxisome matrix targeting signal-2 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016558">
        <property type="term" value="P:protein import into peroxisome matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059104">
        <property type="entry name" value="Beta-prop_EIPR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044536">
        <property type="entry name" value="PEX7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46027">
        <property type="entry name" value="PEROXISOMAL TARGETING SIGNAL 2 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46027:SF1">
        <property type="entry name" value="PEROXISOMAL TARGETING SIGNAL 2 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23609">
        <property type="entry name" value="Beta-prop_EIPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="5 11" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="100"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="EIPR1-like beta-propeller" evidence="12">
        <location>
            <begin position="168"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="237"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="270"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF23609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR25136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="367" mass="41619" checksum="B09A846B09E44079" modified="2009-07-28" version="1">MLRYHTQGYSGYGVQYSPFFDNKVAVATGSNFGLVGNGKLFIIDIDNQGRMLETKSFVTQDGLFDVAWNELHENHVLVAQGDGSLRLFDIQLNDYPVAIFQEHEKEVFSCNWNLINKQLFVSSSWDGTVKVWTPMRQSSLTTLTPKPMTAHNSALSSKDIPMSNQKRHTSLSKNKNCIYQSQFSPHDPNIVMSCAGNSYVTMFDLRQPANANQYSFMAHNGMETLTCDFNKYRSHIIATGGVDNMVKVWDLRMVRKMAANSRQPMSVNEICGHDLAIRKVSWSPHHSNMLLSTSYDMTCRVWQDLSDDGRRPTGKTNSIDPTHGCRFIFSHHTEFVFGADWSLWGQPGYVASTSWDGDVCIWYAFAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMY2</accession>
    <name>C5DMY2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 13">Branchpoint-bridging protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">KLTH0G12584g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Necessary for the splicing of pre-mRNA. Has a role in the recognition of the branch site (5'-UACUAAC-3'), the pyrimidine tract and the 3'-splice site at the 3'-end of introns.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the BBP/SF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555580.1">
        <property type="nucleotide sequence ID" value="XM_002555534.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMY2"/>
    <dbReference type="FunCoup" id="C5DMY2">
        <property type="interactions" value="61"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMY2"/>
    <dbReference type="GeneID" id="8293862"/>
    <dbReference type="KEGG" id="lth:KLTH0G12584g"/>
    <dbReference type="eggNOG" id="KOG0119">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016864_1_1_1"/>
    <dbReference type="InParanoid" id="C5DMY2"/>
    <dbReference type="OrthoDB" id="6777263at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000243">
        <property type="term" value="C:commitment complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045131">
        <property type="term" value="F:pre-mRNA branch point binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048024">
        <property type="term" value="P:regulation of mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02395">
        <property type="entry name" value="KH-I_BBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000024">
        <property type="entry name" value="Branchpoint-bridging protein-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045071">
        <property type="entry name" value="BBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055256">
        <property type="entry name" value="KH_1_KHDC4/BBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032570">
        <property type="entry name" value="SF1-HH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047086">
        <property type="entry name" value="SF1-HH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11208">
        <property type="entry name" value="RNA-BINDING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11208:SF45">
        <property type="entry name" value="SPLICING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22675">
        <property type="entry name" value="KH-I_KHDC4-BBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16275">
        <property type="entry name" value="SF1-HH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 13" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9 13" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="10 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="13" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="7 13" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 11 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="15">
        <location>
            <begin position="285"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="71"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="299"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="445"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="350"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="372"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="400"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="452"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU367126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAR25143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="534" mass="59683" checksum="2AB23A823E6193B7" modified="2009-07-28" version="1">MAMNRKDWESRGRAEVPRDALWGPSAKNSRSNPHNILPTRITGALTQEQIVAYQVMFRIQEITSKLRMNVLNPPAKRSRSPSPPPVYDARGRRTNTREQRYKRKLEEERHRLVEIALKMIPHFVAPDDYKRPTKFQDKYYIPIQKYPEINFMGLLLGPRGNTLRKLQEESGCKIAIRGQGSVKEGKNASELPKGAMNFEEPLHCIISAESEEKIQKGIKACEGVVIRAVTSPEGQNELKRGQLRELAELNGTLREDSRPCPICGLRGHKRFECPNRTTFSQQIVCQRCYQPGHATRDCTFSRPDRGYQRDVSSPAMIYQRNADIGSKRAYRQDDDDDDDESMNPWKRRNQGLNDRNTDFEQTPVSSSYRSRQYRDSHSIDNAEHEYEKSRVPYQGPPGLNNFTSPQNSLSRSPPKHELQGPHKFSNGSSDTAAAFASDRAIPPKEHTVSEDAANSANADQSGASVGDITVPQDDATNNGSEVTFAPPGMYTAPIVNAPPGLNTLAPPAQLFDNVEGDNDRPLAGPPGLTGPPGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMZ0</accession>
    <name>C5DMZ0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0G12782p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0G12782g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555588.1">
        <property type="nucleotide sequence ID" value="XM_002555542.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMZ0"/>
    <dbReference type="FunCoup" id="C5DMZ0">
        <property type="interactions" value="74"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMZ0"/>
    <dbReference type="GeneID" id="8293871"/>
    <dbReference type="KEGG" id="lth:KLTH0G12782g"/>
    <dbReference type="eggNOG" id="ENOG502QRM9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017395_2_1_1"/>
    <dbReference type="InParanoid" id="C5DMZ0"/>
    <dbReference type="OMA" id="PNFFTRY"/>
    <dbReference type="OrthoDB" id="2414723at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31758">
        <property type="entry name" value="BTB/POZ DOMAIN-CONTAINING PROTEIN YLR108C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31758:SF2">
        <property type="entry name" value="BTB_POZ DOMAIN-CONTAINING PROTEIN YLR108C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR25151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="434" mass="48612" checksum="9F85CD13D352F5C7" modified="2009-07-28" version="1">MSLSLSPGPEAVLPSDSMFAIQVGPELFRVSGFSLNSDAPSYFTAHFSRNPSATLVLDRSPRVFHLILQHLQGYAVSVDDEIQFTTLFCDAVYFRLPRLLTLLSTSDYHYTCVGGVHFKLPKHLVSEPGNSPNFFDVTSGSVFLDVERVFRSNNLSRPPPLSPPFLSRSPEYFAKLIALLQGAALDLEPCTRRSLILECRYYRFLRLEQQLVPCRLSWNPFSRAPEILLDLGDVKPNGISLPDPPAAEAVSPGEPTAKRPRRTALTTVQYRRPFIDAESPPRDLTLQVGFPHEAAIFRADNANGFYVALYARLLDKFCFVFHKFLSQHGIDLQSFIRRTSDSCALVLPAILSSCHVEVNKRTYEDLASLLDTPRAAEQDPEHSTYFPSLGRGRSIHLTRSIWQPALQGSTLVMVASKIEAVSSIKYFNVELEFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5DN80</accession>
    <name>C5DN80_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GTP-binding nuclear protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0G14850g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the small GTPase superfamily. Ran family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555678.1">
        <property type="nucleotide sequence ID" value="XM_002555632.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DN80"/>
    <dbReference type="FunCoup" id="C5DN80">
        <property type="interactions" value="1705"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DN80"/>
    <dbReference type="GeneID" id="8293963"/>
    <dbReference type="KEGG" id="lth:KLTH0G14850g"/>
    <dbReference type="eggNOG" id="KOG0096">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041217_13_0_1"/>
    <dbReference type="InParanoid" id="C5DN80"/>
    <dbReference type="OMA" id="FNAWDTA"/>
    <dbReference type="OrthoDB" id="48625at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000054">
        <property type="term" value="P:ribosomal subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00877">
        <property type="entry name" value="Ran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000131">
        <property type="entry name" value="GTP-binding nuclear protein Ran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002041">
        <property type="entry name" value="Ran_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24071:SF0">
        <property type="entry name" value="GTP-BINDING NUCLEAR PROTEIN RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24071">
        <property type="entry name" value="RAN GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00627">
        <property type="entry name" value="GTPRANTC4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51418">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR25241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="214" mass="24311" checksum="52C5520D77889870" modified="2009-07-28" version="1">MSGEVPTFKLVLVGDGGTGKTTFVKRHLTGEFEKKYIATIGVEVHPLSFYTDFGELKFDVWDTAGQEKFGGLRDGYYINAQCGIIMFDVTSRITYKNVPNWHRDLVRVCENIPIVLCGNKVDVKERKVKAKTITFHRKKNLQYYDISAKSNYNFEKPFLWLARKLAGNPQLDFVASPALAPPEVQVDEALMHQYQQEMEQATALPLPDEDDADL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNG2</accession>
    <name>C5DNG2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">26S proteasome regulatory subunit 4 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0G16698g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555760.1">
        <property type="nucleotide sequence ID" value="XM_002555714.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNG2"/>
    <dbReference type="FunCoup" id="C5DNG2">
        <property type="interactions" value="1078"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNG2"/>
    <dbReference type="GeneID" id="8294047"/>
    <dbReference type="KEGG" id="lth:KLTH0G16698g"/>
    <dbReference type="eggNOG" id="KOG0726">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_2_3_1"/>
    <dbReference type="InParanoid" id="C5DNG2"/>
    <dbReference type="OMA" id="QDDTDPM"/>
    <dbReference type="OrthoDB" id="10255768at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010604">
        <property type="term" value="P:positive regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070682">
        <property type="term" value="P:proteasome regulatory particle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080090">
        <property type="term" value="P:regulation of primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000030">
        <property type="entry name" value="26S protease regulatory subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000007">
        <property type="entry name" value="26S proteasome regulatory subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000039">
        <property type="entry name" value="26S proteasome regulatory subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032501">
        <property type="entry name" value="Prot_ATP_ID_OB_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16450">
        <property type="entry name" value="Prot_ATP_ID_OB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0647">Proteasome</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="12">
        <location>
            <begin position="233"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00068880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR25323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="455" mass="50667" checksum="BA14A69F2851876F" modified="2009-07-28" version="1">MAKASSQTSLGKLATSTSSKMGQGASTYGKDKKKKDKQKPKYEPPVQSKIGRKKRKGPATAEKLPSVYPSTRCRLKLLRMERIKDHLLLEEEFVTNSEILKPFEKKQEEEKKQLEEIRGTPLSVGTLEEIIDDDHAIITSPTTPDFYVSILSFVDKELLEPGCSVLLHHKTMSIVGVLQDDTDPMINVMKIDKSPTENYSDIGGLESQIQEIKEAVELPLTHPELYEEMGIKPPKGVILYGAPGTGKTLLAKAVANQTSATFLRIVGSELIQKYLGDGPRLCRQIFKAAGENAPSIVFIDEIDAIGTKRYDSNSGGEREIQRTMLELLNQLDGFDDRGDVKVIMATNKIESLDPALIRPGRIDRKILFENPDITTKRKILAIHTSKMNLATDVDLDNLVTSKDDLSGADIKAMCTEAGLLALRERRMQVTAEDFKQAKERILKNKVEENLEGLYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNK6</accession>
    <name>C5DNK6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">60S acidic ribosomal protein P0</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0G17820g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. uL10 forms part of the P stalk that participates in recruiting G proteins to the ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the universal ribosomal protein uL10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555804.1">
        <property type="nucleotide sequence ID" value="XM_002555758.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNK6"/>
    <dbReference type="FunCoup" id="C5DNK6">
        <property type="interactions" value="1624"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNK6"/>
    <dbReference type="GeneID" id="8294098"/>
    <dbReference type="KEGG" id="lth:KLTH0G17820g"/>
    <dbReference type="eggNOG" id="KOG0815">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053173_1_1_1"/>
    <dbReference type="InParanoid" id="C5DNK6"/>
    <dbReference type="OMA" id="DMNPFKL"/>
    <dbReference type="OrthoDB" id="10259902at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05795">
        <property type="entry name" value="Ribosomal_P0_L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1730:FF:000002">
        <property type="entry name" value="60S acidic ribosomal protein P0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.105.20:FF:000001">
        <property type="entry name" value="60S acidic ribosomal protein P0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.105.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050323">
        <property type="entry name" value="Ribosomal_protein_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001790">
        <property type="entry name" value="Ribosomal_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040637">
        <property type="entry name" value="Ribosomal_uL10-like_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043164">
        <property type="entry name" value="Ribosomal_uL10-like_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043141">
        <property type="entry name" value="Ribosomal_uL10-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030670">
        <property type="entry name" value="uL10_eukaryotes"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45699">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN P0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45699:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00428">
        <property type="entry name" value="Ribosomal_60s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00466">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17777">
        <property type="entry name" value="RL10P_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039087">
        <property type="entry name" value="L10E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160369">
        <property type="entry name" value="Ribosomal protein L10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL10-like insertion" evidence="7">
        <location>
            <begin position="109"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="274"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="274"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="292"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR039087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR25367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="311" mass="33580" checksum="5B9E43FBD7052163" modified="2009-07-28" version="1">MGGIREKKAEYFAKLREYLEEYKSVFVVGVDNVSSQQMHEVRKDLRGRGVVLMGKNTMVRRAIRGFLSDLPDFEKLLPFVKGNVGFVFTNDSLKDIKEVITSNVVAAPARAGGIAPEDIWVRAVNTGMEPGKTSFFQALGVPTKIARGTIEIVSDVKVVDAGTKVGASEAALLNLLNISPFVFGLTVVQVYDNGQVFPSSILDITDEELVKHFVSAVATISAISLAIGYPTLPSVGHSIVNNYKNLLAVAIASKYIYPEIEELADRIENPDKYASAAPVAAADSSSAPAEEAAPEEDEESEDDDMGFGLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNM1</accession>
    <name>C5DNM1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0G18260p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0G18260g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555819.1">
        <property type="nucleotide sequence ID" value="XM_002555773.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNM1"/>
    <dbReference type="GeneID" id="8294114"/>
    <dbReference type="KEGG" id="lth:KLTH0G18260g"/>
    <dbReference type="HOGENOM" id="CLU_2606454_0_0_1"/>
    <dbReference type="InParanoid" id="C5DNM1"/>
    <dbReference type="OMA" id="GIWNDAR"/>
    <dbReference type="OrthoDB" id="4034781at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR25382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="79" mass="9018" checksum="6A0A64438C329AFC" modified="2009-07-28" version="1">MTEASAHKRSPTTPSTPGIWNDARAKKLKSAATQSPTKPRRSRDATVEELTRQQLINSRELQRVNGLVRFLELERKFQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNP5</accession>
    <name>C5DNP5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0G18788p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0G18788g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endomembrane system</location>
            <topology evidence="7">Lipid-anchor</topology>
            <orientation evidence="7">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555843.1">
        <property type="nucleotide sequence ID" value="XM_002555797.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNP5"/>
    <dbReference type="FunCoup" id="C5DNP5">
        <property type="interactions" value="1214"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNP5"/>
    <dbReference type="GeneID" id="8294138"/>
    <dbReference type="KEGG" id="lth:KLTH0G18788g"/>
    <dbReference type="eggNOG" id="KOG0084">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041217_23_1_1"/>
    <dbReference type="InParanoid" id="C5DNP5"/>
    <dbReference type="OMA" id="TQMAKDF"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01869">
        <property type="entry name" value="Rab1_Ypt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000359">
        <property type="entry name" value="Small GTP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057289">
        <property type="entry name" value="Rab1/Ypt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0636">Prenylation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="173"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="176"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="191"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR25406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="204" mass="22903" checksum="2FE672321706C6E0" modified="2009-07-28" version="1">MNSEYDYLFKLLLIGDSGVGKSCLLLRFSDDTYTNDYISTIGVDFKIKTVELDGKTVKLQIWDTAGQERFRTITSSYYRGAHGIIIVYDVTDHDSFNDVKMWLQEIDRYATAGVLKLMVGNKSDLQDKRMVEYDVAKEFADSLQIPFLETSALDSSNVEEAFLTMAKQIKESMAQQHKDTGKKDDKANVNLKGQSLSNASGGCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>C5E229</accession>
    <name>C5E229_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0H01694p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0H01694g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the cyclin family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555952.1">
        <property type="nucleotide sequence ID" value="XM_002555906.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E229"/>
    <dbReference type="FunCoup" id="C5E229">
        <property type="interactions" value="1969"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E229"/>
    <dbReference type="GeneID" id="8294265"/>
    <dbReference type="KEGG" id="lth:KLTH0H01694g"/>
    <dbReference type="eggNOG" id="KOG0653">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020695_12_1_1"/>
    <dbReference type="InParanoid" id="C5E229"/>
    <dbReference type="OMA" id="AHYKHVE"/>
    <dbReference type="OrthoDB" id="5590282at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044772">
        <property type="term" value="P:mitotic cell cycle phase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20512">
        <property type="entry name" value="CYCLIN_CLBs_yeast_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000001">
        <property type="entry name" value="G2/mitotic-specific cyclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046965">
        <property type="entry name" value="Cyclin_A/B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004367">
        <property type="entry name" value="Cyclin_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048258">
        <property type="entry name" value="Cyclins_cyclin-box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02984">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001771">
        <property type="entry name" value="Cyclin_A_B_D_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01332">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00292">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="2 4" id="KW-0195">Cyclin</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="6">
        <location>
            <begin position="224"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin C-terminal" evidence="7">
        <location>
            <begin position="317"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="6">
        <location>
            <begin position="321"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="63"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR30090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="442" mass="50541" checksum="0851FDE11ABB8B3D" modified="2009-07-28" version="1">MQYSRFASDENRVFDHAAHARFTNDENAVPQGPAGLAGSKLALGVAESVQTSGRRVALGDVTSQANKQAHGKPGVLAHAHSRHASENHNGPATAQLEHTGAQPMSPPMELRSPEKMYSDDTLQGADHVGEDFNDDTSSVSSDSEPLLLVSTEESMRAVQLANQEFYSESPDPLDEDTYDLVMVVEYTQEVFQYLHELEEKYRPHPRYMHFQPELKWSYRSTLIDWIVQVHARFQLLPETLYLTVNIIDRFLSRKTVTLNRFQLVGAAALFLAAKFEEINCPTLKEILYMLDNSYTKDELLRAERYMINTLEFELGWPGPMSFLRRVSKADDYEYDIRTLAKYLLETTIMDCRLVSAQPSWLAAGAYYLSRVILGFNNWTKQHIYCSGYTAEQLFPLATIILENCRHASKRHQAIFEKYSERRHRRSSQVVARWIAMAEERIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2A3</accession>
    <name>C5E2A3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0H03322p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0H03322g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556026.1">
        <property type="nucleotide sequence ID" value="XM_002555980.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2A3"/>
    <dbReference type="FunCoup" id="C5E2A3">
        <property type="interactions" value="16"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2A3"/>
    <dbReference type="GeneID" id="8294339"/>
    <dbReference type="KEGG" id="lth:KLTH0H03322g"/>
    <dbReference type="eggNOG" id="KOG2644">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030805_0_0_1"/>
    <dbReference type="InParanoid" id="C5E2A3"/>
    <dbReference type="OMA" id="EGWAPGC"/>
    <dbReference type="OrthoDB" id="448496at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047884">
        <property type="term" value="F:FAD diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042726">
        <property type="term" value="P:flavin-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00885">
        <property type="entry name" value="cinA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="entry name" value="MoaB/Mog-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47675">
        <property type="entry name" value="MOLYBDOPTERIN BINDING DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_5G11210)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47675:SF1">
        <property type="entry name" value="MOLYBDOPTERIN BINDING DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_5G11210)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="Molybdenum cofactor biosynthesis proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MoaB/Mog" evidence="1">
        <location>
            <begin position="22"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR30164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="289" mass="33099" checksum="445C0F8CC9959D5E" modified="2009-07-28" version="1">MLPRFLATQTPKIARMPSVKGACVIIGDEVLNGKITDTNSRFFAKYCYRLGIQLREIVTIGDQKEQIVQTIKRLSAENDFIVTSGGIGPTHDDITYESVASSFGLPCELNYECQDRMRKISHPEKRHDPETLKDFYRMATLPAGPEVHSYYVLKEFWVPIVSIQQKVYILPGIPQLFEKLLLAFEPSVKEIYSISEREPHNYERFFVKTPKSESQISSYLRELQTRCLQVSDEIKIGSYPHYGMGFNTVSILGLKKHEVFLKELVKEAIEKLDGTAITAEMEEKYSDSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2U9</accession>
    <name>C5E2U9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0H07876p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0H07876g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS15 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556222.1">
        <property type="nucleotide sequence ID" value="XM_002556176.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2U9"/>
    <dbReference type="FunCoup" id="C5E2U9">
        <property type="interactions" value="1042"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2U9"/>
    <dbReference type="GeneID" id="8294540"/>
    <dbReference type="KEGG" id="lth:KLTH0H07876g"/>
    <dbReference type="eggNOG" id="KOG0400">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090139_1_0_1"/>
    <dbReference type="InParanoid" id="C5E2U9"/>
    <dbReference type="OMA" id="MHTRRKG"/>
    <dbReference type="OrthoDB" id="623277at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070181">
        <property type="term" value="F:small ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00353">
        <property type="entry name" value="Ribosomal_S15p_S13e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.10:FF:000003">
        <property type="entry name" value="40S ribosomal protein S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.860.130:FF:000001">
        <property type="entry name" value="40S ribosomal protein S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.10">
        <property type="entry name" value="S15/NS1, RNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01343_A">
        <property type="entry name" value="Ribosomal_uS15_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000589">
        <property type="entry name" value="Ribosomal_uS15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023029">
        <property type="entry name" value="Ribosomal_uS15_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012606">
        <property type="entry name" value="Ribosomal_uS15_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009068">
        <property type="entry name" value="uS15_NS1_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006331">
        <property type="entry name" value="PRK08561.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11885">
        <property type="entry name" value="RIBOSOMAL PROTEIN S15P/S13E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11885:SF6">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08069">
        <property type="entry name" value="Ribosomal_S13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00312">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01386">
        <property type="entry name" value="Ribosomal_S13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01387">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47060">
        <property type="entry name" value="S15/NS1 RNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00362">
        <property type="entry name" value="RIBOSOMAL_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS15 N-terminal" evidence="5">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR30360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="151" mass="17135" checksum="DFC0758AD94CE12E" modified="2009-07-28" version="1">MGRMHSSGKGISSSAIPYSRNAPAWFKTSPEAIVEQIIKYARKGLTPSQIGVLLRDAHGVTQTRFITGNKIMRILKSNGLAPEIPEDLYYLIKKAVSVRKHLERNRKDKDAKFRLILIESRIHRLARYYRTVSVLPPNWKYESATASALVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2V7</accession>
    <name>C5E2V7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0H08074p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0H08074g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556230.1">
        <property type="nucleotide sequence ID" value="XM_002556184.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2V7"/>
    <dbReference type="FunCoup" id="C5E2V7">
        <property type="interactions" value="55"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2V7"/>
    <dbReference type="GeneID" id="8294941"/>
    <dbReference type="KEGG" id="lth:KLTH0H08074g"/>
    <dbReference type="eggNOG" id="ENOG502S1BQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_122953_0_0_1"/>
    <dbReference type="InParanoid" id="C5E2V7"/>
    <dbReference type="OMA" id="HLINCFD"/>
    <dbReference type="OrthoDB" id="2565191at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006360">
        <property type="term" value="P:transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022793">
        <property type="entry name" value="Rrn10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28054">
        <property type="entry name" value="RNA POLYMERASE I-SPECIFIC TRANSCRIPTION INITIATION FACTOR RRN10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28054:SF1">
        <property type="entry name" value="RNA POLYMERASE I-SPECIFIC TRANSCRIPTION INITIATION FACTOR RRN10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05234">
        <property type="entry name" value="UAF_Rrn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR30368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="137" mass="15596" checksum="A4C85C880E2CAE45" modified="2009-07-28" version="1">MDRSVYEACSDLVEQFGHKVSADEILAQKVSNLVPIPFKSREDLELAALGDKKEGIYSGELAPRIDLKVLHYYATQLIVQKYPHLINCFDETALLTLGLLVEKWVEDYLRAQSDELRTNPARLIAKKTDYRKSPSDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2X7</accession>
    <name>C5E2X7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ATP-dependent RNA helicase ROK1</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">ATP-dependent RNA helicase rok1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ordered locus">KLTH0H08646g</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the DEAD box helicase family. DDX52/ROK1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556250.1">
        <property type="nucleotide sequence ID" value="XM_002556204.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2X7"/>
    <dbReference type="FunCoup" id="C5E2X7">
        <property type="interactions" value="1120"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2X7"/>
    <dbReference type="GeneID" id="8294574"/>
    <dbReference type="KEGG" id="lth:KLTH0H08646g"/>
    <dbReference type="eggNOG" id="KOG0344">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_1_4_1"/>
    <dbReference type="InParanoid" id="C5E2X7"/>
    <dbReference type="OMA" id="EMAHSIM"/>
    <dbReference type="OrthoDB" id="360161at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17957">
        <property type="entry name" value="DEADc_DDX52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000759">
        <property type="entry name" value="probable ATP-dependent RNA helicase DDX52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044764">
        <property type="entry name" value="DDX52/Rok1_DEADc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF15">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 16" id="KW-0347">Helicase</keyword>
    <keyword evidence="6 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="20">
        <location>
            <begin position="127"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="18">
        <location>
            <begin position="158"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="19">
        <location>
            <begin position="349"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="15"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="517"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="15">
        <location>
            <begin position="127"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="25"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="517"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="547"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="CAR30388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="566" mass="63497" checksum="23792C3D24E70609" modified="2009-07-28" version="1">MDIFQVLTRGANVKKNGKLGASGDFSKSNGSQRKQIQAGSTRKEDDNKQLTKELDFFRNKKVVSKTENAQAGKHDPAEDIAAEVSTLGDEMPPPKISSAHDAVLLRKSYKGNVTGQDLPLPIGSFEDLITRFQFDKRLLSNLIESHFTEPTPIQCEAIPLALEGRDVLACAPTGSGKTVAFILPLLQQIISLGDKEGLKGLIISPTKELANQIFVECSKLSHKIYLARKRPLNIALLSKSLSAKLRNKVISDKKYDLIISTPLRLIDVVKNEALDLSDVKHLIFDEADKLFDSTFVEQADSILASCQDSQLRKSMFSATVPSSLEEVVNSIMLDPVRIIIGHKEAANVNIEQKLVYCGNEEGKLVAIRQMIQEGGFKPPVIIFLESITRAKALFHELLYDNLNVDVIHAERTQVQRNTIIERFRTGELWCLICTDVLARGVDFKGVNLVINYDVPRSAQAYVHRIGRTGRGGREGKAVTFYTKQDDMAIKPIINVMRQSGFEIAEWMEKFAKMTKREKEALKKGKSRPERKQISTVPNVVKKKRRQREQMVEASKKRKEQDDEERS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>C5E326</accession>
    <name>C5E326_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0H09746p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0H09746g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556299.1">
        <property type="nucleotide sequence ID" value="XM_002556253.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E326"/>
    <dbReference type="GeneID" id="8294624"/>
    <dbReference type="KEGG" id="lth:KLTH0H09746g"/>
    <dbReference type="eggNOG" id="ENOG502ST2Q">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_644161_0_0_1"/>
    <dbReference type="InParanoid" id="C5E326"/>
    <dbReference type="OMA" id="GKYWYAL"/>
    <dbReference type="OrthoDB" id="4035180at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR30437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="426" mass="47877" checksum="F914AAB0BF1A2B03" modified="2009-07-28" version="1">MDTLLANIDSGPELLEHSSELQDCSRNLGLEEFASFSKMCRLYHELRETAYGEEMNKLAVAFANVLLKQQGFRAQALAQNAELAEDVQMRLERDSVSSEGFKVEWEVPLWRVLFLLAHGHADGSRSFEVFSRDLVPNKFQHLAEKLAENEQQSSKLPLVFEELGKYWYALCYNYGSFVVTHGAHFWSSLESFTASLRANDGRLLGDYASALLQLCSPLLLFPENNLLATFDMVLNLTLILKHGIIAWSGKVSEELYSSPQDYKIPQILHVIFVFVAKSPHAVRKRIQPCFSGEEGCGVNLRKQVLDLQCSKITCSETRETLDRILSALGLTVSANDDGELEVIHVVQSQPPSARDSTETVYDQRYSQSSSRLSDLSSSGTTLHNSLDGDYDDLWSEEDKIAEADRIMAVIRRLDELGIIKPKFPGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5E369</accession>
    <name>C5E369_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0H10824p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0H10824g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the bacterial ribosomal protein bL17 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556342.1">
        <property type="nucleotide sequence ID" value="XM_002556296.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E369"/>
    <dbReference type="FunCoup" id="C5E369">
        <property type="interactions" value="697"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E369"/>
    <dbReference type="GeneID" id="8294673"/>
    <dbReference type="KEGG" id="lth:KLTH0H10824g"/>
    <dbReference type="eggNOG" id="KOG3280">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074407_1_3_1"/>
    <dbReference type="InParanoid" id="C5E369"/>
    <dbReference type="OMA" id="FQHGSIV"/>
    <dbReference type="OrthoDB" id="275000at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1030.10">
        <property type="entry name" value="Ribosomal protein L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000456">
        <property type="entry name" value="Ribosomal_bL17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036373">
        <property type="entry name" value="Ribosomal_bL17_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040894">
        <property type="entry name" value="Ribosomal_bL17m_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00059">
        <property type="entry name" value="L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14413:SF16">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL17M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14413">
        <property type="entry name" value="RIBOSOMAL PROTEIN L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18502">
        <property type="entry name" value="Mrpl_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01196">
        <property type="entry name" value="Ribosomal_L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64263">
        <property type="entry name" value="Prokaryotic ribosomal protein L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein bL17m C-terminal fungi" evidence="6">
        <location>
            <begin position="123"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="217"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="217"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF18502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR30480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="243" mass="27820" checksum="CF10F9DF8DEAD9A4" modified="2009-07-28" version="1">MTAGLMRHFSRTKAHRDSMFRNLVSQLFQHGSIVSTHEKCLEASRMADRVITMAKKLKHHPNSAMEIQSRLSLAGDNSKLLSRVVGELAPRYKQRPGGYTRVLKLEPRLGDRAKQSVLELVDTPVVDAAGALQRGDIKLWLLCKTTIQNESKSVGYSQLTLRNFAKMLKFRKNSHTFFTNDVLAVRKFIKQEQGLDWDEKHELKLVKQLKKQVLNEQAQPAQTQTQPEAPGYTYVQERPQRQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3G6</accession>
    <name>C5E3G6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0H13376p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0H13376g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556439.1">
        <property type="nucleotide sequence ID" value="XM_002556393.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3G6"/>
    <dbReference type="GeneID" id="8294798"/>
    <dbReference type="KEGG" id="lth:KLTH0H13376g"/>
    <dbReference type="eggNOG" id="KOG0254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_30_1_1"/>
    <dbReference type="InParanoid" id="C5E3G6"/>
    <dbReference type="OMA" id="LAMREDW"/>
    <dbReference type="OrthoDB" id="2241241at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000134">
        <property type="entry name" value="MFS sugar transporter protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF50">
        <property type="entry name" value="HEXOSE TRANSPORTER HXT14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="138"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="161"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="197"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="231"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="333"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="371"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="397"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="431"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="469"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="497"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="59"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR30577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="540" mass="60505" checksum="F0E1F99BA8172656" modified="2009-07-28" version="1">MAGDYLGCEIEESYEIGRSRRGSVPIPSNSKEVGKVESFKTETRESLGLRDQNWDVILACYGISFTGFFFGYDTGTVGGITNMQPWLRRFGHFDASSGEYHMSTALIGLVVSAFHIGCITGGFTIARLADYIGRRAPIAIGCAVYMLGLAIQMSAGQGKWYQYMIGRMVTGLTVGANAVLTPMLLSEIAPPGIRGAIVNFYQINITHGILIGYVVDFATKNHYKDDKMWRLPLIGGFVFSVIILPMLASAPESPRFLIKRGRFDDARKVFARLRGINLKQVEGNMANSQQAQILDVINEELQYLKENYHYQEQRQRNTSFWQLFSKRFLKRTLSGMCIMAFQQMSGIDYFLYYGTKLFKSVGIEDSYKTSIILGAINASMTYVSMFVADNLGRKKGLFLGSLCCFVCLFTFSTVGVTTIDNASDPNYKLSGYIMIVFTCLFIVFFCCSWSAIAPVLVSEIFTLEIKSQAMALSQAFNWGANFFIALCTPIITARIGYAFGYVFTGFMFIAVPFVYFFIPETKGLTLEEIDDYYEKENLCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3H7</accession>
    <name>C5E3H7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">DNA polymerase</fullName>
            <ecNumber evidence="15">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">KLTH0H13662g</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">DNA polymerase that functions in several pathways of DNA repair. Involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA. Also contributes to DNA double-strand break repair by non-homologous end joining and homologous recombination. Has both template-dependent and template-independent (terminal transferase) DNA polymerase activities. Has also a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 15">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 15">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the DNA polymerase type-X family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="15"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002556404.1"/>
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        <property type="interactions" value="298"/>
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    <dbReference type="STRING" id="559295.C5E3H7"/>
    <dbReference type="GeneID" id="8294811"/>
    <dbReference type="KEGG" id="lth:KLTH0H13662g"/>
    <dbReference type="eggNOG" id="KOG2534">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008698_3_1_1"/>
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        <property type="component" value="Chromosome H"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
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        <property type="entry name" value="Beta Polymerase, domain 2"/>
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        <property type="entry name" value="DNA polymerase beta, N-terminal domain-like"/>
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    </dbReference>
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        <property type="entry name" value="DNAPOLX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00870">
        <property type="entry name" value="DNAPOLXBETA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00483">
        <property type="entry name" value="POLXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47802">
        <property type="entry name" value="DNA polymerase beta, N-terminal domain-like"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PsbU/PolX domain-like"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 15" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11 15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 15" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 15" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="BRCT" evidence="16">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile; Schiff-base intermediate with DNA; for 5'-dRP lyase activity" evidence="14">
        <location>
            <position position="239"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
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            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023204"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
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    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR622312-50"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU366014"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAR30588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="558" mass="62782" checksum="BB64A317E74E854F" modified="2009-07-28" version="1">MKLFSGLNFLVIPSLEPPRIQFLRGLIEENGGAVSESTLPGFIILVNDSFVSEYGELTKLELFQSEWDYTDTVWDAISSVRDVEAYRISWISVCLKQRRLIRNADLRISVALRDEEALESRHLTFEDEPEASTEVYTDSETDIGSFNPEGQSQEVTSLAKESIVNVSTGPVGREHCKEINANLIQFLEQLSAKFHRQGDKFRSRSYQLALQSIKKHGKPITSEEGALSLPNIGPSIASKIKLVLDVGSLPGLQLSTEREELLEYFMNCYGVGSHTARKWADGGARTFKDALKLFPSDFSWSILFGWKYYDDWTQRIPRQECAQHLKIVESVLQNVDPQARIVITGSYRRGAATCGDIDMVLYKQDCDSFDELSQILETVVLKLSETGYIVCPLNLNQHILHAMKPCINEVAEAAGLDSYSVTSFKGVLHKFYFGGRITDGQKTTSPLASYSSRLKEADQFLSGSCLENKCRRVDILLCRWAQLGGTMIYFTGNDDFNKSLRLRATKRGWKLSNNGLYQAAQVPGQGDTLLEAFSERRIMELLGVPWVPPEQRNIEGYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3J2</accession>
    <name>C5E3J2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0H14014p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0H14014g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556465.1">
        <property type="nucleotide sequence ID" value="XM_002556419.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3J2"/>
    <dbReference type="FunCoup" id="C5E3J2">
        <property type="interactions" value="53"/>
    </dbReference>
    <dbReference type="GeneID" id="8294827"/>
    <dbReference type="KEGG" id="lth:KLTH0H14014g"/>
    <dbReference type="eggNOG" id="ENOG502S2H3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052849_0_0_1"/>
    <dbReference type="InParanoid" id="C5E3J2"/>
    <dbReference type="OMA" id="YVNFIRY"/>
    <dbReference type="OrthoDB" id="4034134at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038872">
        <property type="entry name" value="Put_GTT3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41807">
        <property type="entry name" value="GLUTATHIONE TRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41807:SF1">
        <property type="entry name" value="GLUTATHIONE TRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="209"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="300"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR30603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="319" mass="35015" checksum="C2C613BCCDDA5EB2" modified="2009-07-28" version="1">MSFKTWKKIELLDLADKLDLHASQASTKAVIANAIDKHLRQLDGPLDLNEFPELATYYEDAERVALEAEAAHSDASETSAVSDGTTSEGTSDGTSDGTSEGTSSEDESEPGHKMPRGTPFQNWFSQLDFSRVTPARGSFAFKFNEFLQDVQSRTIEANENVQDALSTIPAVDVVFLAIEAYVYVISKHLEFSAASPFVAIGSSWREVSALLGFWSVWSLITPALVGYYINFIRYDLPDVPVDPMVFHISKALFALLLAQWQPSFSDEAAYAVKDVAGSATFMDSVAHWSRFALAEWKQQLGLLPFILGCAGSALCLYVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3U1</accession>
    <name>C5E3U1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0H16456p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0H16456g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD(P)H dehydrogenase (quinone) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556564.1">
        <property type="nucleotide sequence ID" value="XM_002556518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3U1"/>
    <dbReference type="STRING" id="559295.C5E3U1"/>
    <dbReference type="GeneID" id="8294937"/>
    <dbReference type="KEGG" id="lth:KLTH0H16456g"/>
    <dbReference type="eggNOG" id="ENOG502QWY5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058643_2_1_1"/>
    <dbReference type="InParanoid" id="C5E3U1"/>
    <dbReference type="OMA" id="HGILHYP"/>
    <dbReference type="OrthoDB" id="26889at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003955">
        <property type="term" value="F:NAD(P)H dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003680">
        <property type="entry name" value="Flavodoxin_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051545">
        <property type="entry name" value="NAD(P)H_dehydrogenase_qn"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10204">
        <property type="entry name" value="NAD P H OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10204:SF34">
        <property type="entry name" value="NAD(P)H DEHYDROGENASE [QUINONE] 1 ISOFORM 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02525">
        <property type="entry name" value="Flavodoxin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like fold" evidence="3">
        <location>
            <begin position="1"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR30702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="264" mass="29848" checksum="27665E3C4D0FF76E" modified="2009-07-28" version="1">MKYFIVHAHPDSHSLTSSLKDVAVQELESQGHEVKVSDLYAQKWKASIDRDDFEQLSQEERLKIPQASYEAYARGALTKDVNQEQEKLIWADFVILSFPIWWSSMPAILKGWVDRVFSCGFTYGVGEHNKSRWGTRYGEGVFSGKRAMLLATFGGSESQYSARGIGGPVEDVLFPINHGILYYPGFTVLPPFVVHNTISVTEKRFQRLSNEMRLYLKSIDSTKPISYRKQNYGDYLIPSLVLRDDIKPGAKGFGIHIGDAQSEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBB1</accession>
    <name>C5DBB1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0A01078p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0A01078g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551510.1">
        <property type="nucleotide sequence ID" value="XM_002551464.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBB1"/>
    <dbReference type="FunCoup" id="C5DBB1">
        <property type="interactions" value="16"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBB1"/>
    <dbReference type="GeneID" id="8290301"/>
    <dbReference type="KEGG" id="lth:KLTH0A01078g"/>
    <dbReference type="eggNOG" id="ENOG502S504">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115063_1_0_1"/>
    <dbReference type="InParanoid" id="C5DBB1"/>
    <dbReference type="OMA" id="LNAWSCV"/>
    <dbReference type="OrthoDB" id="67317at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056552">
        <property type="entry name" value="Ribonucl_Kappa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026770">
        <property type="entry name" value="RNase_K"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31733">
        <property type="entry name" value="RIBONUCLEASE KAPPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23489">
        <property type="entry name" value="V-ATPase_su_f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="51"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR21068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="85" mass="9261" checksum="9837C307C1E02F1B" modified="2009-07-28" version="1">MKPVVSSGKAWFCTVISAFGVVILSVIAHLFNTNHESFVGSINDPEDGPAVARTVFMAVLVYAVFFVFCGSQIWLGKKKPAIELR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBF9</accession>
    <name>C5DBF9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0A02288p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0A02288g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551558.1">
        <property type="nucleotide sequence ID" value="XM_002551512.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBF9"/>
    <dbReference type="FunCoup" id="C5DBF9">
        <property type="interactions" value="383"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBF9"/>
    <dbReference type="GeneID" id="8290355"/>
    <dbReference type="KEGG" id="lth:KLTH0A02288g"/>
    <dbReference type="eggNOG" id="ENOG502QQ1T">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046464_0_0_1"/>
    <dbReference type="InParanoid" id="C5DBF9"/>
    <dbReference type="OMA" id="SAKHEEY"/>
    <dbReference type="OrthoDB" id="5599269at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036286">
        <property type="term" value="C:eisosome filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070941">
        <property type="term" value="P:eisosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000005">
        <property type="entry name" value="Sphingolipid long chain base-responsive pil1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028245">
        <property type="entry name" value="PIL1/LSP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31962:SF3">
        <property type="entry name" value="SPHINGOLIPID LONG CHAIN BASE-RESPONSIVE PROTEIN LSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31962">
        <property type="entry name" value="SPHINGOLIPID LONG CHAIN BASE-RESPONSIVE PROTEIN PIL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13805">
        <property type="entry name" value="Pil1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="280"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR21116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="315" mass="35073" checksum="D38D59564C957B09" modified="2009-07-28" version="1">MHRTYSLRAQRAPTASQLQSPAPPPSSTRSKFFGKASISSTFRKNAAGNFGPDLARKLSQLVKTEKGVLRALEIVAIERRAAAKQLSLWGLDNDDDVSDVTDKLGVLIYELGELQDQFIDKYDQYRLTLKGIRNIEASVQPSRDRKQKITDDIARLKYKEPNSTKIPVLEQELVRAEAESLVAEAQLSNITREKLKAAFNYQFDSIRELAEKFALIAGYGKALLELLDDSPVTPGETRLAYDGYDASRQIIMDAETALEGWTLDSAAVKPSLSFHQTVDDVYEEDDEEEGEAAAAEEEWAGEHEGGEEEVESTRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBS0</accession>
    <name>C5DBS0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0A04884p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0A04884g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551669.1">
        <property type="nucleotide sequence ID" value="XM_002551623.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBS0"/>
    <dbReference type="FunCoup" id="C5DBS0">
        <property type="interactions" value="608"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBS0"/>
    <dbReference type="GeneID" id="8290473"/>
    <dbReference type="KEGG" id="lth:KLTH0A04884g"/>
    <dbReference type="eggNOG" id="KOG2536">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072692_0_1_1"/>
    <dbReference type="InParanoid" id="C5DBS0"/>
    <dbReference type="OMA" id="CEYMMSK"/>
    <dbReference type="OrthoDB" id="278212at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:cytosolic ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.280.10">
        <property type="entry name" value="Mitochondrial glycoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003428">
        <property type="entry name" value="MAM33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036561">
        <property type="entry name" value="MAM33_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10826">
        <property type="entry name" value="COMPLEMENT COMPONENT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10826:SF1">
        <property type="entry name" value="COMPLEMENT COMPONENT 1 Q SUBCOMPONENT-BINDING PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02330">
        <property type="entry name" value="MAM33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54529">
        <property type="entry name" value="Mitochondrial glycoprotein MAM33-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR21227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="262" mass="28919" checksum="65660954CCCFA338" modified="2009-07-28" version="1">MFSRVLVRSAAVAARSARTARCAPMLRSVNLQAASLRPLHFSAVRLNKQCQKVAQVLESEVALEVEEDVQDLPESLQSFFDKTGFSAVASPGKNMAEITSKTARGETVHVFFDVAQVSNLPLESAELDGEAAAPSEEEEFDGMADNFANVNVVVVKDSDQSAVSFELLMNLQEGTFFVDSATPYSSAKAALDETAESEVSRELVYHGPPFSNLDEELQESLEAYLESRGINEELATFIGTYSEFKENKEYINWLKNMKTFFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBS8</accession>
    <name>C5DBS8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">26S proteasome regulatory subunit RPN2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0A05060g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the proteasome subunit S1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551677.1">
        <property type="nucleotide sequence ID" value="XM_002551631.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBS8"/>
    <dbReference type="FunCoup" id="C5DBS8">
        <property type="interactions" value="1529"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBS8"/>
    <dbReference type="GeneID" id="8290481"/>
    <dbReference type="KEGG" id="lth:KLTH0A05060g"/>
    <dbReference type="eggNOG" id="KOG2062">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002323_0_0_1"/>
    <dbReference type="InParanoid" id="C5DBS8"/>
    <dbReference type="OMA" id="IMFGRQE"/>
    <dbReference type="OrthoDB" id="261572at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034515">
        <property type="term" value="C:proteasome storage granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042176">
        <property type="term" value="P:regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000017">
        <property type="entry name" value="26S proteasome non-ATPase regulatory subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016642">
        <property type="entry name" value="26S_Psome_Rpn2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002015">
        <property type="entry name" value="Proteasome/cyclosome_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048570">
        <property type="entry name" value="PSMD1_RPN2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040623">
        <property type="entry name" value="RPN2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10943">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10943:SF2">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13646">
        <property type="entry name" value="HEAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01851">
        <property type="entry name" value="PC_rep"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18004">
        <property type="entry name" value="RPN2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21505">
        <property type="entry name" value="RPN2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015947">
        <property type="entry name" value="26S_Psome_Rpn2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0647">Proteasome</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="26S proteasome non-ATPase regulatory subunit 1/RPN2 N-terminal" evidence="9">
        <location>
            <begin position="5"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="26S proteasome regulatory subunit RPN2 C-terminal" evidence="8">
        <location>
            <begin position="753"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="808"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="817"/>
            <end position="854"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR015947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF21505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR21235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="943" mass="103587" checksum="4C99125A38E67B63" modified="2009-07-28" version="1">MSVVTAAPLLALLNENDNVAKSYALEAINEVVDQLWSEISNEITQIEALYDDNSFQDRKLAALVASKVYYNLGEYESAVKFALAADNYFNLDEKSQFVETIVSQSIDMYIKSSAKNYESSGSEVKIDPQLSSVFERMLEKCVECGDFKLALGIALESYRLDVVERILKARVEQDSDANALKLITYVLTAASTTVTSTPFRTSILHSLFNILMSTKSPEYFTVSKIIVNLNDLNLALGLFKKLAQEEAAEISYQIAYDLVSSATQGLLEGLHSSLAAEGADEKLLDILSGIPTCDYNNTFLFRNKNIDMGLLNKSKSSMDGKFSLFHTAVSVANGFMHAGTTDDSFIRSNLPWLGKAQNWAKFTATASLGVIHKGNLSDGKKIMEPYLPGSRAASRYIKGGSLYGLGLIYAGYGREIIDYMKSHVIENSSSVGDEDVDVLLHGASLGIGLAGMGSANSELYEALKEVLYNDSANSGEASALGMGLIMLGSGDENAVQDMFTYAQETQHGNITRGLAVGLAVINYGRKELADSLIEKMLLHENALLRYGGAYTIALAYAGTGDNKAIKKLLHIAVSDSDDDVRRAAVIALGFVLIRDYTTVPRIVELLAESHNAHVRCGTAFALGISCVGRGLQAAVDVLMPLTKDPVDFVRQAAMIALSLILMQQTEKTNSKVKDINDLFLNVVSNKHQEGLAKFGACVAQGIMNAGGRNVTIQLENVDMGTLDTKAVIGLAMFSQFWYWFPLAHFLSLSFTPTSIIAVRGQDLSIPAFKFNCHTRPDVFEYPPMYEENTDKNVEKVATAVLSTTAKAKARAKKTKKDGKDTVEEKPRRESKSELKPETKEEEKKLEPDQKDDAPFKIKYTNKPYQIENASRVVPQQLKYIAFSKEDRFVPVRKFKGSNGVIVVFDKTPDEPAELIKTTKQKKDVDAPLPTPFKVEDELEFSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBU6</accession>
    <name>C5DBU6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0A05456p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0A05456g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551695.1">
        <property type="nucleotide sequence ID" value="XM_002551649.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBU6"/>
    <dbReference type="GeneID" id="8290499"/>
    <dbReference type="KEGG" id="lth:KLTH0A05456g"/>
    <dbReference type="HOGENOM" id="CLU_2109472_0_0_1"/>
    <dbReference type="InParanoid" id="C5DBU6"/>
    <dbReference type="OrthoDB" id="4033322at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR21253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="123" mass="13293" checksum="FD1FF4E425C376E4" modified="2009-07-28" version="1">MSYNTQDFLEDGDVFSFSLAQGAPASLLPSTPLSGASDLISGDDDLLLQSPFLDAMSRDDDEDHAGDELFALENEPQLLPSYDMTHITAKAPVPSAFECVPAPDDDEFADAAQHNYRLWLAGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBV1</accession>
    <name>C5DBV1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0A05566p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0A05566g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551700.1">
        <property type="nucleotide sequence ID" value="XM_002551654.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBV1"/>
    <dbReference type="FunCoup" id="C5DBV1">
        <property type="interactions" value="1326"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBV1"/>
    <dbReference type="GeneID" id="8290504"/>
    <dbReference type="KEGG" id="lth:KLTH0A05566g"/>
    <dbReference type="eggNOG" id="ENOG502RX84">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033254_1_0_1"/>
    <dbReference type="InParanoid" id="C5DBV1"/>
    <dbReference type="OMA" id="NWLIKDI"/>
    <dbReference type="OrthoDB" id="10264870at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22933">
        <property type="entry name" value="HFD_HFI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024738">
        <property type="entry name" value="Hfi1/Tada1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21277">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21277:SF5">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12767">
        <property type="entry name" value="SAGA-Tad1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="24"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="142"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="36"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="144"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR21258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="431" mass="47544" checksum="46EF0823FB1C2476" modified="2009-07-28" version="1">MSVVQPQNTPVSPGSVHKQTGVTVSAHGQKPGGALTGTPTGTTVPGNQTNRLNIEKMVESLTAALGKESWSKYAQIISLFVLGKLSRKELVNELDLLFSPQQENQGQLMQGSNMSHGTLIRMHNQLLLAILTNSVRGSPLGESTEGSWGFNNGTTHSSKKRSNKHNSQIDTYKKIVMSLPAEDRSRLKTITKEAGKRGFVLCSVLQARLNNIPKIPIVTNPETLKRIKASNLKTPLEWSQEIVNGFSAPLATESYSLPDNDSLYLRMISIAREHGLVGAVDGRCVEIMTLALENYLKNVVESAIDTVRYREKYHSDYYDLNEEGFYQGVAGTDEEVACEGNELTRDLRSITNEEMAQTLSVFPNLVAPTGAYLNLSMCGLLNDDELVTMASTIDDLPEFQEDKPNFTPVDDKNVGSREELNWLIKDILTKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBV7</accession>
    <name>C5DBV7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DNA repair protein RAD14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0A05698g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the XPA family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551706.1">
        <property type="nucleotide sequence ID" value="XM_002551660.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBV7"/>
    <dbReference type="FunCoup" id="C5DBV7">
        <property type="interactions" value="111"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBV7"/>
    <dbReference type="GeneID" id="8290510"/>
    <dbReference type="KEGG" id="lth:KLTH0A05698g"/>
    <dbReference type="eggNOG" id="KOG4017">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053731_0_1_1"/>
    <dbReference type="InParanoid" id="C5DBV7"/>
    <dbReference type="OMA" id="VCHACKE"/>
    <dbReference type="OrthoDB" id="5368863at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000110">
        <property type="term" value="C:nucleotide-excision repair factor 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901255">
        <property type="term" value="P:nucleotide-excision repair involved in interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000715">
        <property type="term" value="P:nucleotide-excision repair, DNA damage recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070914">
        <property type="term" value="P:UV-damage excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21077">
        <property type="entry name" value="DBD_Rad14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.530.10:FF:000003">
        <property type="entry name" value="Dna repair rad14 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.530.10">
        <property type="entry name" value="XPA C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000465">
        <property type="entry name" value="XPA/RAD14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022656">
        <property type="entry name" value="XPA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022658">
        <property type="entry name" value="XPA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037129">
        <property type="entry name" value="XPA_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00598">
        <property type="entry name" value="rad14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10142">
        <property type="entry name" value="DNA REPAIR PROTEIN COMPLEMENTING XP-A CELLS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10142:SF0">
        <property type="entry name" value="DNA REPAIR PROTEIN COMPLEMENTING XP-A CELLS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05181">
        <property type="entry name" value="XPA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00753">
        <property type="entry name" value="XPA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="XPA C-terminal" evidence="12">
        <location>
            <begin position="201"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="18"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="62"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="29"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="71"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="83"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00072989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF05181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR21264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="349" mass="40659" checksum="0963940E9744A6BA" modified="2009-07-28" version="1">MNAEQKARIEANRQRALEKLKKRGIIRSEQAEKIESRNEPRPKPAAPELAQSVAENKAKALERYKHHQQNLRDASSNINQSVELDKSSGRKRPLDRIRPTVRKQDYIEYDLATMKNSYGGFINAEEDFSEGPENKKQQSLEEWQKAQKERKALYENAPPPDHPSLAPRCIECHINTELDPLLHDVFKLQICKACAKAIPEKYSLLTKTECKEDYFLTDPELNDQELFHRFEKPNPHSGTFARMQLFVRCEVEEFAYKKWGGEEGLDNEWQRREEGKAQRREKKYQAKMKEMRVKTRAQEYTKKLLERKHGKEHVHEFSTAIDGGLNEEGIKLSKRRCIGCGLEVNEIAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBW8</accession>
    <name>C5DBW8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Uridine kinase</fullName>
            <ecNumber evidence="6">2.7.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0A05940g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>cytidine + ATP = CMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17562"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.48"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>uridine + ATP = UMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16825"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16704"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.48"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 6">Pyrimidine metabolism; CTP biosynthesis via salvage pathway; CTP from cytidine: step 1/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uridine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the uridine kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.48" evidence="6"/>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551717.1">
        <property type="nucleotide sequence ID" value="XM_002551671.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBW8"/>
    <dbReference type="FunCoup" id="C5DBW8">
        <property type="interactions" value="863"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBW8"/>
    <dbReference type="GeneID" id="8290521"/>
    <dbReference type="KEGG" id="lth:KLTH0A05940g"/>
    <dbReference type="eggNOG" id="KOG4203">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021278_0_2_1"/>
    <dbReference type="InParanoid" id="C5DBW8"/>
    <dbReference type="OMA" id="EPQLHCE"/>
    <dbReference type="OrthoDB" id="738517at2759"/>
    <dbReference type="UniPathway" id="UPA00574">
        <property type="reaction ID" value="UER00637"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00579">
        <property type="reaction ID" value="UER00640"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043771">
        <property type="term" value="F:cytidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004849">
        <property type="term" value="F:uridine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044211">
        <property type="term" value="P:CTP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044206">
        <property type="term" value="P:UMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd02023">
        <property type="entry name" value="UMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000339">
        <property type="entry name" value="Uridine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006083">
        <property type="entry name" value="PRK/URK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000764">
        <property type="entry name" value="Uridine_kinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004018">
        <property type="entry name" value="PRK05480.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00235">
        <property type="entry name" value="udk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10285">
        <property type="entry name" value="URIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00485">
        <property type="entry name" value="PRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14681">
        <property type="entry name" value="UPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00988">
        <property type="entry name" value="URIDINKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 6" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribulokinase/uridine kinase" evidence="8">
        <location>
            <begin position="47"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="9">
        <location>
            <begin position="283"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="23"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF14681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR21275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="491" mass="54470" checksum="550A859075E37502" modified="2009-07-28" version="1">MGGLESNSNSNLSLFIGPEDGSQELRRTGDPADHGPRYIPPWTTPYVIGVGGSSGSGKTSVAAKIISSINTPWTVLISLDNFYKPLTQEQREQAFQNNFDFDEPDSIDLDLAYECIRSLKEGKKTSIPLYSFVHHNRVPGKSVTIYGSSVIVVEGIYALHDKRLLDLMDLKVFVDVDLDVCLARRLSRDIISRGRELDGCIQQWEKFVKPNAEKYVNPSMKNADVIIPSMGNNAVATQTVINHIKSRLQVKSRKHLQELRNLGHTKELKPLSEMGNVHQLEHTSQVVALKTILLDKYTPRDDFVFYFDRIASILISRALDDMPLHPQKQITTPTGIKVTAPSVHFDKVASINIVRSGDCFMRSLKKAVPGMAMGKLLIQSDSNTGEPQLHCEFLPPGLENYEQVLLADAQIISGAAVIMAIQVLLDHGVPLSKIKVLVYLATEIGIRRIVNAFSGSVTIHAGEIVATEALGTHHCKWARKRFVDSQYFGCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBX3</accession>
    <name>C5DBX3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="21">KLTH0A06050p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="21" type="ordered locus">KLTH0A06050g</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>N(6)-biotinyl-L-lysyl-[protein] + hydrogencarbonate + ATP = N(6)-carboxybiotinyl-L-lysyl-[protein] + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13501"/>
            <dbReference type="Rhea" id="RHEA-COMP:10505"/>
            <dbReference type="Rhea" id="RHEA-COMP:10506"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83144"/>
            <dbReference type="ChEBI" id="CHEBI:83145"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.14"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>hydrogencarbonate + acetyl-CoA + ATP = malonyl-CoA + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11308"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.4.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>biotin</name>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.</text>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551722.1">
        <property type="nucleotide sequence ID" value="XM_002551676.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DBX3">
        <property type="interactions" value="897"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBX3"/>
    <dbReference type="GeneID" id="8290526"/>
    <dbReference type="KEGG" id="lth:KLTH0A06050g"/>
    <dbReference type="eggNOG" id="KOG0368">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000395_5_2_1"/>
    <dbReference type="InParanoid" id="C5DBX3"/>
    <dbReference type="OMA" id="PTPKGHC"/>
    <dbReference type="OrthoDB" id="14612at2759"/>
    <dbReference type="UniPathway" id="UPA00655">
        <property type="reaction ID" value="UER00711"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003989">
        <property type="term" value="F:acetyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004075">
        <property type="term" value="F:biotin carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042759">
        <property type="term" value="P:long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001295">
        <property type="term" value="P:malonyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd06850">
        <property type="entry name" value="biotinyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.460.10:FF:000001">
        <property type="entry name" value="Acetyl-CoA carboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000005">
        <property type="entry name" value="Acetyl-CoA carboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000005">
        <property type="entry name" value="Acetyl-CoA carboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1770.10:FF:000001">
        <property type="entry name" value="acetyl-CoA carboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000003">
        <property type="entry name" value="acetyl-CoA carboxylase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000005">
        <property type="entry name" value="acetyl-CoA carboxylase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000010">
        <property type="entry name" value="acetyl-CoA carboxylase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.460.10">
        <property type="entry name" value="Biotin dependent carboxylase carboxyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1770.10">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049076">
        <property type="entry name" value="ACCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049074">
        <property type="entry name" value="ACCA_BT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034733">
        <property type="entry name" value="AcCoA_carboxyl_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013537">
        <property type="entry name" value="AcCoA_COase_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005481">
        <property type="entry name" value="BC-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001882">
        <property type="entry name" value="Biotin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011764">
        <property type="entry name" value="Biotin_carboxylation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005482">
        <property type="entry name" value="Biotin_COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011763">
        <property type="entry name" value="COA_CT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011762">
        <property type="entry name" value="COA_CT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45728:SF3">
        <property type="entry name" value="ACETYL-COA CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45728">
        <property type="entry name" value="ACETYL-COA CARBOXYLASE, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08326">
        <property type="entry name" value="ACC_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21385">
        <property type="entry name" value="ACCA_BT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02785">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00289">
        <property type="entry name" value="Biotin_carb_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01039">
        <property type="entry name" value="Carboxyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00878">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50979">
        <property type="entry name" value="BC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00188">
        <property type="entry name" value="BIOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50989">
        <property type="entry name" value="COA_CT_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50980">
        <property type="entry name" value="COA_CT_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0092">Biotin</keyword>
    <keyword evidence="9" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="5 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Biotin carboxylation" evidence="18">
        <location>
            <begin position="59"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="17">
        <location>
            <begin position="217"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoyl-binding" evidence="16">
        <location>
            <begin position="695"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="domain" description="CoA carboxyltransferase N-terminal" evidence="19">
        <location>
            <begin position="1483"/>
            <end position="1819"/>
        </location>
    </feature>
    <feature type="domain" description="CoA carboxyltransferase C-terminal" evidence="20">
        <location>
            <begin position="1823"/>
            <end position="2138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1195"/>
            <end position="1214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1198"/>
            <end position="1214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="CAR21280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="2230" mass="248754" checksum="B172C996F7AD1F57" modified="2009-07-28" version="1">MSEESLSEVGVSQKDQYEVTDYTSKHASLAPHFLGLNTVEKAEDSPLKEFVKCHGGHTVISKVLIANNGIAAVKEIRSVRKWAYETFGNERAVQFVAMATPEDLEANAEYLRMADQYVEVPGGTNNNNYANVDLIVEIAERADVDAVWAGWGHASENPLLPERLAKSPRKVIFIGPPGNAMRSLGDKISSTIVAQHASVPCIPWSGTGVDQVHLDKENGLVSVTDDVYQKGCCDSPEDGLAKAKQIGFPVMVKASEGGGGKGIRKVEREEDFIPLYKQAANEIPGSPIFIMKLAGKARHLEVQLLADQYGTNISLFGRDCSVQRRHQKIIEEAPVTIAKPDTFSEMERSAVRLGKLVGYVSAGTVEYLYSHDDDKFYFLELNPRLQVEHPTTEMVTGVNLPAAQLQIAMGIPMHRVKDIRLLYGVDPHTATEIDFDFSSKDSKQIQRKPTPKGHCTACRITSEDPNEGFKPSGGSLHELNFRSSSNVWGYFSVSSSGGIHSFSDSQFGHIFAFGENRQASRKHMVVALKELSIRGDFRTTVEYLIKLLETEDFEGNSITTGWLDDLISQKMTAEKPDPTLAVICGAATKVFIASENGRKEFITSLQKGQVPNKSLLQTMYPVEFIHDGKRYKFTVAKSADDRYTLFINGSKCEVGVRKLSDGGLLIAVGGRSHTIYWKEEVSATRLSVDSMTTLLEVENDPTQLRTPSPGKLVKFLVENGDHVNAGQPYAEVEVMKMQMPLVSQESGIVQLLKQPGSTVSAGDILAILALDDPTKVKHAMPFEGMLPDMGAPIVEGTKPAYKFRSLVSTLENILNGYDNQVIMNASLQQLIEVLRNPQLPYSEWKMQSSALHSRLPIKLDEQLEQLVNRSLSRNAHFPARQLGKMLEAAQKESDDPLFGTVIEPLLDISTRYADGLEAHEHSVFAKFLQNYYQVEKLFSGTNVREEDVILKLRDENAENLDRVALTVLSHSRVSAKNNLILAILKHYQPLCKMSSEVSSRISEPLKQIVELESKATAKVALQAREILIQAALPSVRERTDQIEHILRSSVVQTAYGAFDNKRTDPDLEILKDLIDSNYVVFDVLTQFLTHRDPSVAAAAAEVYIRRAYRAYTIGDLKHYNHANNAVVEWKFQLPSAAFASVPQMKSKLGMHRAISVSDLTYVSEGDNQPLRTGILIASTHLDDVDEGLSKGLQLVPHHTSSSGPVPDRSGSNATLSNVANVSVSSTDGFESEEEVLRRLREILDMNKQQLVEASIRRITFIFGYEDGTYPKYFTFRGPSYAEDETIRHIEPALAFQLELGRMSNFNIKQLFTENRNIHVYEATGKNSAVDKRFFTRGIIRTGRISDDITIQEYLTSEANRLMSNILDNLEVIDTSNSDLNHIFIHFSAVFDVSPEDVEAAFGGFLERFGKRLLRLRVAAAEIRIIIKDPQTGAPVPLRALINNVSGYVVKTELYTEVKNAQGEWVFKSLDKPGSMHLRPIATPYPAKESLQPKRYKAHLMGTTYVYDFPELFRQATVSQWKKISPKTKLSDEFFIANELIEEEDGELTEIDREAGANTIGMVAFKVTVKTPEYPRGRQFVIVANDITFKIGSFGPQEDAFFNKVTEYARKRGIPRIYLSANSGARIGVAEELIPLFNVSWNDPENPAKGFQYLYLSSEGMAELKKQAKDASVVTERVVEDGEERFVIKSIIGADDGLGVECLKGSGLIAGATSRAYKDIFTITLVTCRSVGIGAYLVRLGQRAIQIEGQPIILTGAPAINKLLGREVYSSNLQLGGTQIMYNNGVSHLTAEDDLAGVQKIMEWLSYVPARRDLPVPILETEDKWDREVDFTPSTSEPYDVRWMIEGRNTPEGFEYGLFDKGSFQETLSGWAKGVVVGRARLGGIPLGVIGVETRMVENMIPADPANPVSTESLIQEAGQVWYPNSAFKTAQAINDFNYGEQLPLMIMANWRGFSGGQRDMYNEVLKYGSFIVDALVGYKQPIFTYIPPTGELRGGSWVVVDPTINAEQMEMYADVNSRAGVLEPEGMVGIKYRREKLLATMARLDETYKNLKTQMSDPGLSVEKHQEIATKLAAREKMLMPIYHQITVQFADLHDRSGRMVAKGVISKELDWPEARRFFFWRLRRRLNEEYLMKRLNTELPNAPRLEKIARLSSWYPASVDREDDRIVANWIEENYSVLEEHLKALKVESFAQNLAKSIRSDHENAISGLSEVLKLLSAEDKAKILNSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>C5DC78</accession>
    <name>C5DC78_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0B00770p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0B00770g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551827.1">
        <property type="nucleotide sequence ID" value="XM_002551781.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DC78"/>
    <dbReference type="GeneID" id="8290651"/>
    <dbReference type="KEGG" id="lth:KLTH0B00770g"/>
    <dbReference type="eggNOG" id="ENOG502SX82">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2050076_0_0_1"/>
    <dbReference type="InParanoid" id="C5DC78"/>
    <dbReference type="OMA" id="INCNANP"/>
    <dbReference type="OrthoDB" id="10402956at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR21389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="123" mass="13449" checksum="6EF2EB8CF2DEE17B" modified="2009-07-28" version="1">MGPSAKQCQTVLPCGGLYNLTITSNTSVMEGYRCGSTERSGVDDRDELLSIEIGGINCNANPAFDLYISENTTVIDGYRCGLDSTNYTEYSQCSNTKENGASNLSPRWTHIFVFVSLFAYLFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5DC95</accession>
    <name>C5DC95_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0B01210p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0B01210g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551844.1">
        <property type="nucleotide sequence ID" value="XM_002551798.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DC95"/>
    <dbReference type="GeneID" id="8290670"/>
    <dbReference type="KEGG" id="lth:KLTH0B01210g"/>
    <dbReference type="eggNOG" id="ENOG502SXEX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_839566_0_0_1"/>
    <dbReference type="InParanoid" id="C5DC95"/>
    <dbReference type="OMA" id="ACAIRCT"/>
    <dbReference type="OrthoDB" id="4034296at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="178"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="310"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="313"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="331" mass="37146" checksum="AD662E2D881CBF86" modified="2009-07-28" version="1">MALDLTPDQWERLTAWIRDRSGLPDSEALTLFQDFVLELLRNLHSCNERKWLEEQLSGLVDNPTEFLRDLEIFLKGLGASAPPSLNSGTPFVLVAHVPYKNLNAQDVQAAFAPFGAIVSCRADVDSRSLLVQFRKVACAIRCTKAATLFFSNRFVTVELYQGDPESFGSVRLIGSAPQPVAADSDPVPPSAAKPSPVPFNDRVQQVQTAQQTIFEQNQRSAEAYKHNFAQLFESKEKLLRAHQAALQELKQKVLATEDPASISRTIIEFQELQKNMESLGITPIAMVQLKLQKFNLDDPSEFPVESPRALAVKQKRTKKAASFRRKIKRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCA3</accession>
    <name>C5DCA3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">LSM2-LSM8 complex subunit LSM8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LSM8</name>
        <name evidence="11" type="ordered locus">KLTH0B01408g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Plays role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex). The heptameric LSM2-8 complex binds specifically to the 3'-terminal U-tract of U6 snRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">LSm subunits form a heteromer with a doughnut shape.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551852.1">
        <property type="nucleotide sequence ID" value="XM_002551806.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCA3"/>
    <dbReference type="FunCoup" id="C5DCA3">
        <property type="interactions" value="479"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCA3"/>
    <dbReference type="GeneID" id="8290679"/>
    <dbReference type="KEGG" id="lth:KLTH0B01408g"/>
    <dbReference type="eggNOG" id="KOG1784">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076902_8_1_1"/>
    <dbReference type="InParanoid" id="C5DCA3"/>
    <dbReference type="OMA" id="NTLSCTM"/>
    <dbReference type="OrthoDB" id="422364at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005688">
        <property type="term" value="C:U6 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01727">
        <property type="entry name" value="LSm8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034103">
        <property type="entry name" value="Lsm8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044642">
        <property type="entry name" value="PTHR15588"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15588">
        <property type="entry name" value="LSM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15588:SF9">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 9" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="10">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR21414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="110" mass="12110" checksum="4F2D6629A9697660" modified="2009-07-28" version="1">MSPLLKDFLNKRIVVVTTDGQCVKAMLEGFDKSTNLLLSDVRDRVSGDVVASSYLVRGNQVVCCGLLEEESSPQPDFGAESVSAQLKDTKNIVPDEHLIWQRVWGMKAGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCC5</accession>
    <name>C5DCC5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Sulfate adenylyltransferase</fullName>
            <ecNumber evidence="7">2.7.7.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">ATP-sulfurylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Sulfate adenylate transferase</fullName>
            <shortName evidence="7">SAT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">MET3</name>
        <name evidence="10" type="ordered locus">KLTH0B01914g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the first intracellular reaction of sulfate assimilation, forming adenosine-5'-phosphosulfate (APS) from inorganic sulfate and ATP. Plays an important role in sulfate activation as a component of the biosynthesis pathway of sulfur-containing amino acids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>sulfate + ATP + H(+) = adenosine 5'-phosphosulfate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:18133"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58243"/>
            <dbReference type="EC" id="2.7.7.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 7">Homohexamer. Dimer of trimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The oligomerization domain is distantly related to APS kinases, but it is not functional and does not bind APS. It is required for oligomerization of the enzyme, although the oligomerization state has no effect on the catalytic activity of the enzyme.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the sulfate adenylyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.4" evidence="7"/>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21436.1"/>
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        <property type="nucleotide sequence ID" value="XM_002551828.1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="STRING" id="559295.C5DCC5"/>
    <dbReference type="GeneID" id="8290702"/>
    <dbReference type="KEGG" id="lth:KLTH0B01914g"/>
    <dbReference type="eggNOG" id="KOG0636">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022950_1_0_1"/>
    <dbReference type="InParanoid" id="C5DCC5"/>
    <dbReference type="OMA" id="EWFSFPE"/>
    <dbReference type="OrthoDB" id="468at2759"/>
    <dbReference type="UniPathway" id="UPA00140">
        <property type="reaction ID" value="UER00204"/>
    </dbReference>
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        <property type="component" value="Chromosome B"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="term" value="P:sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin)"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ATP-Sase_PUA-like_dom"/>
    </dbReference>
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        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
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        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
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        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR024951">
        <property type="entry name" value="Sulfurylase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002650">
        <property type="entry name" value="Sulphate_adenylyltransferase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00339">
        <property type="entry name" value="sopT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42700">
        <property type="entry name" value="SULFATE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42700:SF1">
        <property type="entry name" value="SULFATE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01747">
        <property type="entry name" value="ATP-sulfurylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14306">
        <property type="entry name" value="PUA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0198">Cysteine biosynthesis</keyword>
    <keyword evidence="1 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ATP-sulfurylase PUA-like" evidence="9">
        <location>
            <begin position="3"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="Sulphate adenylyltransferase catalytic" evidence="8">
        <location>
            <begin position="171"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-terminal" evidence="7">
        <location>
            <begin position="1"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Catalytic" evidence="7">
        <location>
            <begin position="167"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Required for oligomerization; adenylyl-sulfate kinase-like" evidence="7">
        <location>
            <begin position="393"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="195"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="196"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="197"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="194"/>
            <end position="197"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="288"/>
            <end position="291"/>
        </location>
        <ligand>
            <name>ATP</name>
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        </ligand>
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        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>sulfate</name>
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        </ligand>
    </feature>
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        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="7">
        <location>
            <position position="200"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="7">
        <location>
            <position position="203"/>
        </location>
    </feature>
    <feature type="site" description="Induces change in substrate recognition on ATP binding" evidence="7">
        <location>
            <position position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00062002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03106"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF01747"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF14306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR21436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="505" mass="56644" checksum="213DA80C626D9B41" modified="2009-07-28" version="1">MPAPHGGVLQDLIARDAQIKDQLLQEAAQASIKWDLTPRQICDLELIQNGGFSPLSGFMNQKDYDGVVEKSRLSNGLVWTIPINLDVDEAFAKQLKPDARVVLLQDNEIPVAILTVTDVYKPDKQNEAKKVFRGDPEHPAVKYLKETAGEYYVGGEIQAIQYPVHYDYPGLRKTPAQLRLEFDSKQWDRIVAFQTRNPMHRAHRELTVRAAREHNAKVLIHPVVGLTKPGDIDHHTRVRVYQEIIKRYPNGMAQLSLLPLAMRMGGDREAVWHAIIRKNYGATHFIVGRDHAGPGKNSAGVDFYGAYDAQELVESYKNELGIEVVPFRMVTYLPEEDRYAPIDQIDLSTTSTLNISGTELRNRLRSGGPIPEWFSYPEVVKILRESNPPRPKQGFAIVLSSSLKSNKQLSTALLTTFLQFGGGRHFKVLEHNNDANVLGLVPDFISSGAGLIIPSQYEKWNSVSNAYLVGTSEDADITLDSDDEAVLHVVQKVVLFLEDHGFFTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCI6</accession>
    <name>C5DCI6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0B03366p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0B03366g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="CAR21497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="KEGG" id="lth:KLTH0B03366g"/>
    <dbReference type="eggNOG" id="ENOG502S0GN">
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    <dbReference type="HOGENOM" id="CLU_043443_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCI6"/>
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        <property type="component" value="Chromosome B"/>
    </dbReference>
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        <property type="term" value="C:nuclear outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070762">
        <property type="term" value="C:nuclear pore transmembrane ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030474">
        <property type="term" value="P:spindle pole body duplication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="InterPro" id="IPR012578">
        <property type="entry name" value="Nucl_pore_cmplx"/>
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    <dbReference type="PANTHER" id="PTHR28003">
        <property type="entry name" value="NUCLEOPORIN POM34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28003:SF1">
        <property type="entry name" value="NUCLEOPORIN POM34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08058">
        <property type="entry name" value="NPCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="389" mass="43090" checksum="4E11FFACD71416BE" modified="2009-07-28" version="1">MSNSAMDNSFEPSSSNSAFSALYRRDFGNAMNSSTVPRKMFSTPVKPVTQERFASVREKVVRESPYLYKSKILSSPVKDGKRVSKSVGGTPLARPTVFQKSRPTSSESTSNETEAKGTVASENTESVLGGFENPVLAQYSCRIVNKELETRKIISNLLAVLVWNLALKFLSLYLDHTLHGAGFSKRVAKWVQETLVYRINPYADFEHSRWARLITLANVSHIFHLIIFYNVTVSLWRLLVKSQNVKIDDLHLNQRQKQLLGLSDQTQTSSALPRVVLAASPQEPLSKPTSLASSPHKASAPPVFLFKSLQTPLKSKERAAAAERAGVRSERAPKINAFGDLRSSVLKKSSLTPSHVSSGLLPTAMNRTGYIPSSKYAYMRDSPSPRKTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCL7</accession>
    <name>C5DCL7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Chromosome segregation in meiosis protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0B04092g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Plays an important role in the control of DNA replication and the maintenance of replication fork stability.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the fork protection complex (FPC) consisting of TOF1 and CSM3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the CSM3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551966.1">
        <property type="nucleotide sequence ID" value="XM_002551920.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCL7"/>
    <dbReference type="FunCoup" id="C5DCL7">
        <property type="interactions" value="282"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCL7"/>
    <dbReference type="GeneID" id="8290801"/>
    <dbReference type="KEGG" id="lth:KLTH0B04092g"/>
    <dbReference type="eggNOG" id="KOG3004">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068300_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCL7"/>
    <dbReference type="OMA" id="DWDFMNV"/>
    <dbReference type="OrthoDB" id="437078at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031298">
        <property type="term" value="C:replication fork protection complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000076">
        <property type="term" value="P:DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043111">
        <property type="term" value="P:replication fork arrest"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031297">
        <property type="term" value="P:replication fork processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012923">
        <property type="entry name" value="Csm3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040038">
        <property type="entry name" value="TIPIN/Csm3/Swi3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13220">
        <property type="entry name" value="TIMELESS INTERACTING-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13220:SF11">
        <property type="entry name" value="TIMELESS-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07962">
        <property type="entry name" value="Swi3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4 8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0236">DNA replication inhibitor</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chromosome segregation in meiosis protein 3" evidence="10">
        <location>
            <begin position="59"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="154"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="208"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="220"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="246"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="259"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR21528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="278" mass="30540" checksum="FBCDE23F952DD868" modified="2009-07-28" version="1">MSQQLQERFSDGTALSQDPDSSLLVDPTAINPSGLGPDGDAPLQDPTAVTTRVRRPQVKLTAEKLCSAKGLPAVMKHAPRRLRISKRRSSYDNLCHLLQFYQLWAHDLYPKAKFHDFVELCRNLGKTDPQLREYRIELLRQELEMGSALEEGAPAFPQERSPAPEPTPDAPGIADLRSEPPSPAPAPAGADSDDDLIYNITRRAPARVLHDSDIDEDSLMETQPPASSTAGDAPRSAESGPGPILNNNNNNNNNNKNNDDDDDDEDEDELAVMREMGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCQ5</accession>
    <name>C5DCQ5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Histone acetyltransferase</fullName>
            <ecNumber evidence="3 12">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0B04994g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the MYST (SAS/MOZ) family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="3 12"/>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552004.1">
        <property type="nucleotide sequence ID" value="XM_002551958.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCQ5"/>
    <dbReference type="FunCoup" id="C5DCQ5">
        <property type="interactions" value="177"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCQ5"/>
    <dbReference type="GeneID" id="8290842"/>
    <dbReference type="KEGG" id="lth:KLTH0B04994g"/>
    <dbReference type="eggNOG" id="KOG2747">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014892_0_2_1"/>
    <dbReference type="InParanoid" id="C5DCQ5"/>
    <dbReference type="OMA" id="TDYNLSC"/>
    <dbReference type="OrthoDB" id="787137at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990467">
        <property type="term" value="C:NuA3a histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004402">
        <property type="term" value="F:histone acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.60:FF:000001">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000001">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.60">
        <property type="entry name" value="N-acetyl transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002717">
        <property type="entry name" value="HAT_MYST-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050603">
        <property type="entry name" value="MYST_HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040706">
        <property type="entry name" value="Zf-MYST"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10615">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10615:SF161">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE KAT7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01853">
        <property type="entry name" value="MOZ_SAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17772">
        <property type="entry name" value="zf-MYST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51726">
        <property type="entry name" value="MYST_HAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYST-type HAT" evidence="14">
        <location>
            <begin position="213"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="610"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="639"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="677"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="713"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="11">
        <location>
            <position position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR602717-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR21566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="740" mass="85251" checksum="5DCEAF7DE9843BC9" modified="2009-07-28" version="1">MGSKDVNSQRHQRLLRNIEITDNVHAVTANEIARNPDGYARQTRAKLAYHPRDEKTELQVKPNLQWSLPDDAKEANALSSHVEFIKVDPVRGTFNKHIVKLRYDPRKLLSFDNLINNAAPDPQKYHVDFEIREPETLFNRIDNECSKPYRCAIGDRRDYSTQRTTPSLKDREFFQSLLHKAAPASYYNGNLLLAFNEAEEREITNPKKNKSRKAPSKIEYIYFRDHEIKTWYTAPYPEEFNKNKILYICEYCLKYMNSRYVIHRHQLKCSLRHPPGNEIYREGNISIWEVDGRENVIYCQNLCLLAKLFLNSKTLYYDVEPFIFYVLTEREDTGEESQFHFVGYFSKEKLTSTDYNLSCILTLPIYQRKGYGHFLVDFSYLLTRREYKWGTPEKPLSDLGLLSYRNFWKTKICEVLGELKEEINHAEKKGEILHCSIEDLSNLTGMIPTDVVFGLEQIGALHYYKGESKLQFAIKIDNWSLIDEVNNARKSKGYRALVPEKLVWKPMIFGPSCGINAMGTMIETTTGAARSTATGQQNGIDTFKNSVSVLVNFLRDDIEDPRSMEAICQQKIHDQQGRHFDTSKINLMTLAFNAPVSRRLKNSASSLVSVPNRYDDNETNNNTFSAPNGGADLGIVDPELLEETEQIDEEDEDNDLNETLADGDTIGDEEVKFSSTSEDEEEDEDEEIPGRGVSSSQGRFSRTRSGRAINSHRVIDSDEHEEEHIATRRLRSMDPLLKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCR0</accession>
    <name>C5DCR0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">KLTH0B05104p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0B05104g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">P-body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Nudix hydrolase family. DCP2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552009.1">
        <property type="nucleotide sequence ID" value="XM_002551963.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCR0"/>
    <dbReference type="FunCoup" id="C5DCR0">
        <property type="interactions" value="361"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCR0"/>
    <dbReference type="GeneID" id="8290847"/>
    <dbReference type="KEGG" id="lth:KLTH0B05104g"/>
    <dbReference type="eggNOG" id="KOG2937">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009571_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCR0"/>
    <dbReference type="OrthoDB" id="18996at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140933">
        <property type="term" value="F:5'-(N(7)-methylguanosine 5'-triphospho)-[mRNA] hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03672">
        <property type="entry name" value="NUDIX_Dcp2p_Nudt20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000045">
        <property type="entry name" value="mRNA-decapping enzyme 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1050">
        <property type="entry name" value="Dcp2, box A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007722">
        <property type="entry name" value="DCP2_BoxA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036189">
        <property type="entry name" value="DCP2_BoxA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044099">
        <property type="entry name" value="Dcp2_NUDIX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23114">
        <property type="entry name" value="M7GPPPN-MRNA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23114:SF17">
        <property type="entry name" value="M7GPPPN-MRNA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05026">
        <property type="entry name" value="DCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01125">
        <property type="entry name" value="DCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140586">
        <property type="entry name" value="Dcp2 domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0464">Manganese</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9" id="KW-0866">Nonsense-mediated mRNA decay</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="12">
        <location>
            <begin position="101"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="285"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="350"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="695"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="820"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="285"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="359"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="385"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="424"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="453"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="477"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="529"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="606"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="695"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="706"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="824"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="838"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="865"/>
            <end position="875"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR21571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="893" mass="98632" checksum="7533E92D20D2A2E4" modified="2009-07-28" version="1">MSLPLRRPLESVTSLDRVLEDLIVRFIINCPPEDFSSVERELFHVEEATWFYTDFVKLMNPKMPNMKIKSFAQHVIKLCPMVWKWDVRAEQALQKFSQYKRSIPVRGAAIFNRSLTKLLLVKGIESESWSFPRGKISKDEGDVDCCIREVLEEIGFDISDYIDEDQFIERTIAGKNYKIFLVSGVPEDFDFQPQVRNEIEKIQWHDFKKLSRNNGNKQHVKIYLVNTLMRPLAMWVKSHRQIDNDDKLKAHVETQLKLLLGIKKEETPDPGRELLNMLQSSVAAPQEALAAAPQSPSELPPSSKSSQDSTPATNAAPPMKSPPPQLHHNFPFMLFQPFAPFPFMAGAGMQTGGPVPGLDTSSSNSVQTQPPPTPSMNTLAKPTVAEEEEEEEEQQQQQRDSQQFQLLEPPRPQSTTSKQLLDLLNRKSSEKAEPTEQQTTKPKFKILKRGESLTPTPSAPQNDNEADSKALLNLLKKPQEPSITRETEDAQPTTMIEKPIATPLEDLPHEEEASLHFANTHSAAAASESEYEDFESSSEEEDIGNDEEEAKASSLKGEGNFGQKFEHTSTNPNVGYGHDQTRISGPMQGLKEQSSGAADVPHKDSSNLSEQSTGGTFITQSSKSQTKPKIKLLKRGESLKDMIFTNGNKTLETSEASHVVKTTSHEGESNELLKLLKKDAISSTADFTNAEAAIHRDSTSARRGTEGSSPLSTTVNQHVTPGQQPISSALTGSDDAKDLLNILHRKNQASQSYQLQSTPEIQQALCTVDTSRGNSSMAAGDFLNMLPNGSSVSSPTAAGNFLPGMEAQFRNPYYNQYPTSSGVPIPPQFPVQPPYAQSPPQNQNSSQQLLGMLKNPHAGIAASPAVNSASTSDTPFVSEKGASHELLSLLQRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCT1</accession>
    <name>C5DCT1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0B05588p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0B05588g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="protein sequence ID" value="CAR21592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002551984.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCT1"/>
    <dbReference type="FunCoup" id="C5DCT1">
        <property type="interactions" value="55"/>
    </dbReference>
    <dbReference type="GeneID" id="8290869"/>
    <dbReference type="KEGG" id="lth:KLTH0B05588g"/>
    <dbReference type="eggNOG" id="ENOG502S517">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128329_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCT1"/>
    <dbReference type="OMA" id="HEVPENR"/>
    <dbReference type="OrthoDB" id="4036151at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019331">
        <property type="entry name" value="FAM192A/Fyv6_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10187">
        <property type="entry name" value="FAM192A_Fyv6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAM192A/Fyv6 N-terminal" evidence="4">
        <location>
            <begin position="21"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="112"/>
            <end position="180"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="14"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="160"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR21592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="180" mass="21018" checksum="755E07DC3C7C2DF6" modified="2009-07-28" version="1">MSTRQENDLQSSRFVKEGNADIELQRQRERQESAKLDTDFRTKERLTLQDQLRLNAITKQEEFSSLVKDRNSFNRLSKQDVEFYESLQRRAQLENQSLEEFLEKNTREYDKKKAQVLAHESGRPESVPTRVQVPAKITKKRSMPSKNGIKGVIKKKKSKSNHEVPENRDHSKVETSKGSN</sequence>
</entry>
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        </submittedName>
    </protein>
    <gene>
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    </gene>
    <organism evidence="7 8">
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        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
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            <taxon>Dikarya</taxon>
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        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
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                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
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                <person name="Talla E."/>
            </authorList>
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            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="protein sequence ID" value="CAR21649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002552041.1"/>
    </dbReference>
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    <dbReference type="KEGG" id="lth:KLTH0B06908g"/>
    <dbReference type="eggNOG" id="ENOG502S5S1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_771766_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCY8"/>
    <dbReference type="OMA" id="WHKSESH"/>
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    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
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        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd12087">
        <property type="entry name" value="TM_EGFR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051694">
        <property type="entry name" value="Immunoregulatory_rcpt-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15549:SF26">
        <property type="entry name" value="AXIAL BUDDING PATTERN PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15549">
        <property type="entry name" value="PAIRED IMMUNOGLOBULIN-LIKE TYPE 2 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="180"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="15"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
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            <begin position="72"/>
            <end position="94"/>
        </location>
    </feature>
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        <location>
            <begin position="223"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="257"/>
            <end position="359"/>
        </location>
    </feature>
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        <location>
            <begin position="300"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="323"/>
            <end position="338"/>
        </location>
    </feature>
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        <location>
            <begin position="349"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR21649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="359" mass="38266" checksum="AACBED29C3166151" modified="2009-07-28" version="1">MLFNATIVSFSSRSRVGTSSSSTQSSSEKEPQTSSSVWSSLELSTLESTSTQTIVSTSASITAVEPSATMLTSSDDSITTTTSDTSLPAATSSLPSSSVPAGKTAVVYTRSYVFTASSTTFGTKLPMTTVMATKKAATFSAPTAAVTTDLSFYQNWLSGTLGSTSSSSSGSLSKTHRNTIIGSVVGSVCGFLLVLGFLWFVLCRRRKNRASQSRSFSHEIGTRLDYPVAPPRPSQTQVPEDDEAFLKSKYKSLGRKLPLWHKSESHPTRGGTAEAESAQGEPKGNPFQDEFDFQRRLSRPPAPPPVPPRDVPNLSHFADDSDASSSSSENSVNDSSSSIQLTRPARAARSTQSFLHEVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCZ4</accession>
    <name>C5DCZ4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 7">Pre-rRNA-processing protein IPI3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0B07040g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">Component of the RIX1 complex required for processing of ITS2 sequences from 35S pre-rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the RIX1 complex, composed of IPI1, RIX1/IPI2 and IPI3 in a 1:2:2 stoichiometry. The complex interacts (via RIX1) with MDN1 (via its hexameric AAA ATPase ring) and the pre-60S ribosome particles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the WD repeat IPI3/WDR18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552093.1">
        <property type="nucleotide sequence ID" value="XM_002552047.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCZ4"/>
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    </dbReference>
    <dbReference type="STRING" id="559295.C5DCZ4"/>
    <dbReference type="GeneID" id="8290933"/>
    <dbReference type="KEGG" id="lth:KLTH0B07040g"/>
    <dbReference type="eggNOG" id="KOG0646">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029749_4_0_1"/>
    <dbReference type="InParanoid" id="C5DCZ4"/>
    <dbReference type="OMA" id="WEAHYNK"/>
    <dbReference type="OrthoDB" id="756370at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005656">
        <property type="term" value="C:nuclear pre-replicative complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
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        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
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        <property type="entry name" value="WD40_repeat_dom_sf"/>
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        <property type="entry name" value="WD40_rpt"/>
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        <property type="entry name" value="WDR18/Ipi3/RID3"/>
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    <dbReference type="PANTHER" id="PTHR18763:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 18"/>
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    <dbReference type="PANTHER" id="PTHR18763">
        <property type="entry name" value="WD-REPEAT PROTEIN 18"/>
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        <property type="entry name" value="WD40 repeat-like"/>
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        <property type="entry name" value="WD_REPEATS_2"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="4" id="KW-0677">Repeat</keyword>
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    <keyword evidence="3 6 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="125"/>
            <end position="157"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
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            <end position="467"/>
        </location>
    </feature>
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            <begin position="447"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002355"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010143"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00026229"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU369067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR21655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="506" mass="55426" checksum="773A54D06DB57336" modified="2009-07-28" version="1">MDEQLIFSTNTTCTVGSLHSPNQTNLKQCSNEFRNGSCLVGSKYLLVAQARKALINVYRIEGSGKRESVEQRLPLPETVNCLEVVENNAEQNGNSQLPYLLLASTSSGKLYVWEMASGNLLTVKAMAHYQAITKIRSIIQGKYVITSGADARIMIWQTVDLVSQQDPKPLFTLHDHTLAVTDFEVSNAHSSQLSGKLFTVSHDMTMRCYDLNMDVFDKPQLLTTFTFPFPLECITLDPADRACYVGGAQGVYALPFYYPIGDRKVANLLSSDSSNILSCVEQQPGDKNADLFTMGKVALAKITNAQTSQLACSLEGSLLVVGDSLGKCTVMDVYSKQPVKEIQAIVANETAGEVTSIILNPVNVDNSNSLLGDGKTQSGFKIPNLQKNVFAREGLHDILFQIEDLQDSKVAPLDDFEAYLDQVAAEEGTFAQLGNVVSTVKVMSESEGANRNPENPDTAQRPADDGELLTMKATVGQLTGAYKELREMYDKLFQEHESLRSTLGEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5DD07</accession>
    <name>C5DD07_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0B07326p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0B07326g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002552060.1"/>
    </dbReference>
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    <dbReference type="eggNOG" id="ENOG502RZI4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061420_1_1_1"/>
    <dbReference type="InParanoid" id="C5DD07"/>
    <dbReference type="OMA" id="HRACNTS"/>
    <dbReference type="OrthoDB" id="4065952at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008253">
        <property type="entry name" value="Marvel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37451">
        <property type="entry name" value="MARVEL DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37451:SF1">
        <property type="entry name" value="MARVEL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01284">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="36"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="66"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="143"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="7">
        <location>
            <begin position="3"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="200"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="200"/>
            <end position="215"/>
        </location>
    </feature>
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        <location>
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            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="238"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="287"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR21668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="307" mass="33537" checksum="340B9EFC6F156B1B" modified="2009-07-28" version="1">MLISLRALQFISSVIALGLLAYVLKGYSYHGSHKTNFGLAVAAISVFYLMLLSLLGVLLHKLLLPGLYLLFEIIITILWLCAFVVLAKTHGSRACGLQTTSTYNPNYGSTSSFMSSGGRYDPYTSQYTTDSHMRPCRSAKASIAFAGLCTVLFMISTLIIGLNVIRPIMQRGGGQRAMWTPYHNAGYKLNPWTGLRLSETNRSDLEGTGPHHNDAEGGAGVHDQHTYSTGDSTYNGTNREKLGETDHRRNASGATEMGQDATNTGNAGALPQENVNTTYHRTERIVTDTSPHPITETNRAQQTQGRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5DD37</accession>
    <name>C5DD37_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0B08008p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0B08008g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002552090.1"/>
    </dbReference>
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    <dbReference type="FunCoup" id="C5DD37">
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    </dbReference>
    <dbReference type="STRING" id="559295.C5DD37"/>
    <dbReference type="GeneID" id="8290976"/>
    <dbReference type="KEGG" id="lth:KLTH0B08008g"/>
    <dbReference type="eggNOG" id="KOG0371">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023125_0_1_1"/>
    <dbReference type="InParanoid" id="C5DD37"/>
    <dbReference type="OMA" id="WLDTCPV"/>
    <dbReference type="OrthoDB" id="10267127at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000298">
        <property type="term" value="F:endopolyphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006798">
        <property type="term" value="P:polyphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050126">
        <property type="entry name" value="Ap4A_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42850">
        <property type="entry name" value="METALLOPHOSPHOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42850:SF4">
        <property type="entry name" value="ZINC-DEPENDENT ENDOPOLYPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="1">
        <location>
            <begin position="2"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="249" mass="28688" checksum="D88FEA7B64E16207" modified="2009-07-28" version="1">MFEEFAKLMAKVNAKDENTHVVLLGDFTSKGPQSRAVVDYMREHQDSTHCILGNHEVNVMFAYLNHVGLEQRLSSGHARWRPLEFSSESYIPPRGQVNRMHRDVARELGEERLDWVAKHCPAELAIEFGPQTLVAVHAGLDPDLSREDVPKVKDITTMKYMLPEDHSKTSKFKFKHSRRWYKFWDGQHAPRNTTVFYGHDSSNGLTLRKRTKGLDSACVSGGQLSALEVVYRDGEYQEFLHQVECREMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDB2</accession>
    <name>C5DDB2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0B09768p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0B09768g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552211.1">
        <property type="nucleotide sequence ID" value="XM_002552165.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDB2"/>
    <dbReference type="FunCoup" id="C5DDB2">
        <property type="interactions" value="1212"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDB2"/>
    <dbReference type="GeneID" id="8291055"/>
    <dbReference type="KEGG" id="lth:KLTH0B09768g"/>
    <dbReference type="eggNOG" id="KOG2171">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003794_0_1_1"/>
    <dbReference type="InParanoid" id="C5DDB2"/>
    <dbReference type="OMA" id="PKRFVQE"/>
    <dbReference type="OrthoDB" id="543373at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058584">
        <property type="entry name" value="IMB1_TNPO1-like_TPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040122">
        <property type="entry name" value="Importin_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041653">
        <property type="entry name" value="Importin_rep_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040928">
        <property type="entry name" value="Importin_rep_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041389">
        <property type="entry name" value="Importin_rep_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057672">
        <property type="entry name" value="TPR_IPO4/5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10527">
        <property type="entry name" value="IMPORTIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13513">
        <property type="entry name" value="HEAT_EZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18808">
        <property type="entry name" value="Importin_rep_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18816">
        <property type="entry name" value="Importin_rep_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18829">
        <property type="entry name" value="Importin_rep_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25574">
        <property type="entry name" value="TPR_IMB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25780">
        <property type="entry name" value="TPR_IPO5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="TOG" evidence="8">
        <location>
            <begin position="363"/>
            <end position="595"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR21773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1092" mass="121283" checksum="EF2FDDC751A14941" modified="2009-07-28" version="1">MSALPDEVNTTLVNVLHGFSSPDNGIRAAAEETLNKDWITPDNIEVLLIFLSEQAAYSPEPTLAALSAVLFRKLALRAPPSSKTVIIAKNITHISESALNQIRETLLKGFVTERQGGIRHKLSDAIAECAQEDLPEWPALLQGLFEAIKNPDANFRESSFRIFTSVPHLINAVDINSVLPIFEAGFTDSDDNVKVAAVTAFVGYFKQLPKQHWAKLGILLPSLLNSLPNFLDGGKDDALASVFESLIELVELAPKLFKNMFDQMIQFTDIVIKNKDLETSARTTALELLTAFSESAPQMCKSTPSYAQSLIMNTLLMMTEVSIDDDQASDWHDSDDTEEDDEEVTYDHARQALDRVSLKLGGKYLAPPLFQYLQQMVSSSEWRERFGALMALSSAAEGCRDVLIGEIPKILDMIVPLINDPHPRVQYGCCNALGQISTDFAPLIQRTSHERILPPLISKLTPQSVDRVQTHAAAALVNFSEHATQTILEPYLDDLLTNLLTLLQSTKFYVQEQALTTIAFIAEAAEKKFIKYYDTLMPLLINVLKTDTGNANRLLKGKCIECSTLIALAVGKEKFLTQSQELIELFIAYQNQGIDDDDPMKSYLEHGWSRICRILREDFVPLLPVVIPPLLETAKATQDVSLIEEEDAANFQQYSDWDVVQIQGKHIAIHTSILDDKVSAMELLQVYTTILKNFFAVYVPEIMKEIAVPSIDFYLHDGVRATGAGLIPVLLSALVSATGVQNSDVLQLWHLASTKLLNGIISEPMPEITQIYHTALVDGLAIMGENSLSDEQLLQYTKGLSANLSDIYERVKQRRNQEDEYNEDVDDDFEDYTDEDLLDDINKSLAAVFKSTGGRYLNQFQTLWPLVVTYLRDGEVILTLFALCAIGDMIETAGPATEVYKDSFINEVKEYLVSPEASIRQAAAYVIGVCAQHSRDVYAQECSQSLDTLVRVISIPDAKSEENVTSTENASAAIAKILHSYGPNVPNFDNYVSNWLKTFPIVQDEEAAAFNYRFLAHLIDSNSPAVHNSAAIPHILDSVVQALHHKSLVGKNASAVVESTKKLLGTLPQSEAMNLFQRYPSDVVQEVQHWFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDI2</accession>
    <name>C5DDI2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Malate dehydrogenase</fullName>
            <ecNumber evidence="3 12">1.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0C01188g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 12">
            <text>(S)-malate + NAD(+) = oxaloacetate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21432"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.37"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the LDH/MDH superfamily. MDH type 1 family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.37" evidence="3 12"/>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552281.1">
        <property type="nucleotide sequence ID" value="XM_002552235.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDI2"/>
    <dbReference type="FunCoup" id="C5DDI2">
        <property type="interactions" value="932"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDI2"/>
    <dbReference type="GeneID" id="8291139"/>
    <dbReference type="KEGG" id="lth:KLTH0C01188g"/>
    <dbReference type="eggNOG" id="KOG1494">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047181_1_0_1"/>
    <dbReference type="InParanoid" id="C5DDI2"/>
    <dbReference type="OMA" id="ASCAEYI"/>
    <dbReference type="OrthoDB" id="4069699at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030060">
        <property type="term" value="F:L-malate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006108">
        <property type="term" value="P:malate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01337">
        <property type="entry name" value="MDH_glyoxysomal_mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000013">
        <property type="entry name" value="Malate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.110.10:FF:000001">
        <property type="entry name" value="Malate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.110.10">
        <property type="entry name" value="Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001557">
        <property type="entry name" value="L-lactate/malate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022383">
        <property type="entry name" value="Lactate/malate_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001236">
        <property type="entry name" value="Lactate/malate_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015955">
        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001252">
        <property type="entry name" value="Malate_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010097">
        <property type="entry name" value="Malate_DH_type1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01772">
        <property type="entry name" value="MDH_euk_gproteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11540">
        <property type="entry name" value="MALATE AND LACTATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11540:SF73">
        <property type="entry name" value="MALATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02866">
        <property type="entry name" value="Ldh_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00056">
        <property type="entry name" value="Ldh_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000102">
        <property type="entry name" value="Lac_mal_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56327">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00068">
        <property type="entry name" value="MDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10 12" id="KW-0520">NAD</keyword>
    <keyword evidence="5 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Lactate/malate dehydrogenase N-terminal" evidence="13">
        <location>
            <begin position="19"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="Lactate/malate dehydrogenase C-terminal" evidence="14">
        <location>
            <begin position="165"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="195"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="24"/>
            <end position="30"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="135"/>
            <end position="137"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR21843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="333" mass="35078" checksum="4382F937DF99A576" modified="2009-07-28" version="1">MFAKVAKRAFSSSTANPYKVTVLGAGGGIGQPLSLLLKLNHKVTDLRLYDLKGAAGVAADLSHIPTNSVVKGFSADAQDGIKSALKDTDVVLIPAGVPRKPGMTRDDLFSINASIVRDLAAACAENAPNAAILVISNPVNSTVPIVAEVLKSKGVYNPKKLFGVTTLDVIRASRFISEVSGTNPTTEKVNVIGGHSGITIIPLISQTKHKLMDKEKRDALIHRIQFGGDEVVKAKNGAGSATLSMAQAGARFANSVLAGLEGEADVIEPSFVDSPLFKSEGIEFFASPVKLGPQGVEKIFSIGEISSEEQELLDKCKETLKKNIEKGTAFVKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDV1</accession>
    <name>C5DDV1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0C04004p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0C04004g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552400.1">
        <property type="nucleotide sequence ID" value="XM_002552354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDV1"/>
    <dbReference type="FunCoup" id="C5DDV1">
        <property type="interactions" value="480"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDV1"/>
    <dbReference type="GeneID" id="8291264"/>
    <dbReference type="KEGG" id="lth:KLTH0C04004g"/>
    <dbReference type="eggNOG" id="ENOG502QSAC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010320_0_0_1"/>
    <dbReference type="InParanoid" id="C5DDV1"/>
    <dbReference type="OMA" id="FFTFKFP"/>
    <dbReference type="OrthoDB" id="2283785at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039634">
        <property type="entry name" value="Bul1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022794">
        <property type="entry name" value="Bul1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007519">
        <property type="entry name" value="Bul1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31904">
        <property type="entry name" value="BYPASS OF STOP CODON PROTEIN 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31904:SF1">
        <property type="entry name" value="BYPASS OF STOP CODON PROTEIN 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04426">
        <property type="entry name" value="Bul1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04425">
        <property type="entry name" value="Bul1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bul1 N-terminal" evidence="2">
        <location>
            <begin position="67"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="Bul1 C-terminal" evidence="3">
        <location>
            <begin position="608"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="16"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="131"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR21962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="883" mass="99493" checksum="DE4D00AB8707ED9C" modified="2009-07-28" version="1">MHNERRPRFELGKQDPSTGRGRSSSPSLFFKSSSSSNLLRLRKAHSPSKEPRSTSSSRATLDSLVCNKEQNSASSSSSNMMVDVLPSFELYNTLHRHIPQGNVNPDLHDYPPTYQEVQSRAPTLTPDSHETTTVANASSTSVDSTAHGGRSSISGDQLLNLEALSTLHEPIQDDLGDSDNIYIDKLYSLPKLTTPIEISIRVTKEPAVADRKVDDESILKEYTSGDIIHGYVVIENRSTQRQKFEMFYVTLEGRATSVDRECGKRTVKRFLRMVDISASWSYSNIDVSNGVSYVPGRRDFENCVIGLNNNRVLEPHVRYKKYFMFKLPTHLLDVSCKHQQFSHCLVPPAFGIDVTKASGKYSSIKVNPMLGYGHLGTKGSPILTNDLSTEDVSINYTIDTKVVGKDSKSKKLNILKEREYNLRFIPFGFCRPFSGERAPLNQLQDMSKLIQERIEALKKVFKRLDTNECITSEDLHNTDISGTAVDPTDVDSEDIMRRKLDQLYVSNRVDPVCSSFPLRSSKINKDKDKKVEVEFKYNYKDKQKSANKLKKGIFGSFGPSTQSHSTQPTSSNKSGIIIITSKIPKRGLPYVEPSLLKKTNKLESKSEQNQENWYNLQASLPDKEKEILSSFDIGLRCIQANNCKSHLPPQIQTVTTELICITAKSINSIPIKLNADLLLKKERLDDIQKVFKAFKAEMVELKAKFDENFEQLNELFSKSRRSSIHQELKFTDFLSEQLMTNIESICTLRTDVRSLHNIFKKQSQTLENDDKISDSAPNKSASTASILSNTFSGSSSNSSVHTSTAEKLKNQLLHEWVKTGDGEYERTVTVNLHMNDDIKETLIPTFESCLISRLYCIRVNIKFEGQLGVATVDVPVQIRMLEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDX7</accession>
    <name>C5DDX7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0C04642p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0C04642g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552426.1">
        <property type="nucleotide sequence ID" value="XM_002552380.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDX7"/>
    <dbReference type="FunCoup" id="C5DDX7">
        <property type="interactions" value="66"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDX7"/>
    <dbReference type="GeneID" id="8291293"/>
    <dbReference type="KEGG" id="lth:KLTH0C04642g"/>
    <dbReference type="eggNOG" id="ENOG502QVG0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_351657_0_0_1"/>
    <dbReference type="InParanoid" id="C5DDX7"/>
    <dbReference type="OMA" id="MHESERF"/>
    <dbReference type="OrthoDB" id="4061518at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="775" mass="89132" checksum="F8DC10B91A9B1DFC" modified="2009-07-28" version="1">MSSVKSPHGFLQFLRPFRLRESCIQRGIFLRRYNPPQNDRSKDVLREWAKSVLHESSPESASECSFDKEIQLSTQIDRATETSLKNGSVFPDHQERAKEPSARAIAQSTRGSSPLKTFSMSTLSNIASSLQKSHKFQEIDSIYKTHRNWLSSGVVDRSSRAYNEFVQAVFAAEYRLQNFTLCENLFSECLKFRPVSPEVIDMGLITFVKNNNLTLAKEFYVQILKNPETFPMTPNTLHAFAFEIFKTSDLATMERVISLWLENSSSPKTYPFHKTLALFHRLLLKLGDEEGIAKLLSHSGVIKTKYEGSTEFDLCLLHHEVAARMLTDHVKIGHRMDEISSKMSNEKRSKVYIEVLKLGVAKNDFSLIKLATVKAQEDSFVNLNDEFHKHVCRYFVKNGLLKSLVQYLNDAVLPKADCRFGQIYIEQLWNCALQNYPMLSREFTNDIQLLLNKEELIREHEWLEYTTLRKLKNFSSNHGLQKSPTQTLSSLCCRLGPEFANAIQTSLADGDASAIRSLILNELQRGVRPDFTVFYSLLKLLMPDYIDSAKLVDQIMRETYREIPLKIDILWLKHNALEATKSIRTNASTPAGKQQAALVAATKIKDFENQHSGRLNFQNYMQLSSLFLVLRDARNASHLLEKGKKLINSSNKRDWYIYYSNALKVYTRARDPGKFLSVLKDWNANDSAWLITPDTIRSCKGYMKYFERNQGLTAHNSATLFEIHSEIDSMLARYVNFKFQGLNDMKMVSSFLQRWIEQDLKKKKENHLSDVQSEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDY2</accession>
    <name>C5DDY2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0C04752p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0C04752g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552431.1">
        <property type="nucleotide sequence ID" value="XM_002552385.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDY2"/>
    <dbReference type="FunCoup" id="C5DDY2">
        <property type="interactions" value="100"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDY2"/>
    <dbReference type="GeneID" id="8291298"/>
    <dbReference type="KEGG" id="lth:KLTH0C04752g"/>
    <dbReference type="eggNOG" id="KOG4510">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032828_4_1_1"/>
    <dbReference type="InParanoid" id="C5DDY2"/>
    <dbReference type="OMA" id="PIASCYV"/>
    <dbReference type="OrthoDB" id="306876at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911">
        <property type="entry name" value="ACYL-MALONYL CONDENSING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911:SF6">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER G1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="31"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="162"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="200"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="235"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="264"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="294"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="319"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="6">
        <location>
            <begin position="31"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="6">
        <location>
            <begin position="203"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR21993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="372" mass="41691" checksum="B19686B8F8D56628" modified="2009-07-28" version="1">MTEVPRSETAGNKSNDSRLQRLSSEYLKPNIGLLYLLISNLFNSVMVVSTKLLETDPELEEPITPLQILVVRMFITYIGTLIYMLYNRDRIDHVPWGPPDMRKWLILRGCTGFFGVFGMYYSLMYLSVPDATIITFLGPSFTGLLAWAILRERYSKVEATGALVSLMGVILIVRPSFLFGSSATDDSGGTAESRDPHARLVATIVALVGVIGASNVYIIIRYIGQRAHAIMSVSYFALIACCVSLVGICVIPSMGFQIPQTSKQWFLFTLIGFSGFFMQLLLTMGIQRERAGRGAIMSYSQIIYAIFWDVFIWHHLPGLWSWCGIIVILGSAIWVVKCKPKSEFIATATTDQESQVIQMEDFEIHDDEDDIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDY8</accession>
    <name>C5DDY8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">KLTH0C04884p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0C04884g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552437.1">
        <property type="nucleotide sequence ID" value="XM_002552391.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDY8"/>
    <dbReference type="STRING" id="559295.C5DDY8"/>
    <dbReference type="GeneID" id="8291304"/>
    <dbReference type="KEGG" id="lth:KLTH0C04884g"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_811503_0_0_1"/>
    <dbReference type="InParanoid" id="C5DDY8"/>
    <dbReference type="OrthoDB" id="6077919at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000060">
        <property type="entry name" value="zinc finger protein 436"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003:SF20">
        <property type="entry name" value="FINGER DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G10380)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR PROTEIN YY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="238"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="266"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="110"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="172"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="113"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="172"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR21999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="342" mass="37356" checksum="9D455E55CABFFFEC" modified="2009-07-28" version="1">MAKSQLHEEPQRVPAFWETYQPLDGSRVAGDLFTSFKSSEEVLSLTSHHALFQQDQMHMYTDGLATNPLVFGDSHFQFQDHGEHGGHDSCDAHDARDSYAYISGSSSCDELPPAHAQPHGAGASAGPLTFSDLEESDSTDADALFVAEPCGRSRCALPARSDSFDLAQLLYSHSSSPSRPPWSLVDAQKRNKSTNDVASGRRSSHCRAKRPSFATLDAGKTASEDARAVSKLKLQPRYVCASCGKCFNRPSSLATHNNTHTGDKPFTCPFENCDKQFNARSNMTRHYKLHFKTEGGTYLLPNGEVTRARPPMRQLVGMQSNVAASDLISFSETSEDLPLAGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2023-06-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>C5DE56</accession>
    <name>C5DE56_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0C06424p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0C06424g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552505.1">
        <property type="nucleotide sequence ID" value="XM_002552459.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DE56"/>
    <dbReference type="GeneID" id="8291373"/>
    <dbReference type="KEGG" id="lth:KLTH0C06424g"/>
    <dbReference type="HOGENOM" id="CLU_2360094_0_0_1"/>
    <dbReference type="InParanoid" id="C5DE56"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR22067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="96" mass="10698" checksum="94BD58C41C701D93" modified="2009-07-28" version="1">MIKFYYVTFSFTTQTNNTNTLNELVETQMWKQAIPSQTATIGSNSFEENHVSMLSSSQASQLITMPASTMSPNLCNTGESLIKLESLLPYKQAVHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEG1</accession>
    <name>C5DEG1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0C08888p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0C08888g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SF3A3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552610.1">
        <property type="nucleotide sequence ID" value="XM_002552564.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEG1"/>
    <dbReference type="FunCoup" id="C5DEG1">
        <property type="interactions" value="1343"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEG1"/>
    <dbReference type="GeneID" id="8291484"/>
    <dbReference type="KEGG" id="lth:KLTH0C08888g"/>
    <dbReference type="eggNOG" id="KOG2636">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027160_1_1_1"/>
    <dbReference type="InParanoid" id="C5DEG1"/>
    <dbReference type="OMA" id="GPKAFQK"/>
    <dbReference type="OrthoDB" id="2160351at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000690">
        <property type="entry name" value="Matrin/U1-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031590">
        <property type="entry name" value="PRP9_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051421">
        <property type="entry name" value="RNA_Proc_DNA_Dmg_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031774">
        <property type="entry name" value="SF3A3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024598">
        <property type="entry name" value="SF3a60/Prp9_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022755">
        <property type="entry name" value="Znf_C2H2_jaz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12786:SF2">
        <property type="entry name" value="SPLICING FACTOR 3A SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12786">
        <property type="entry name" value="SPLICING FACTOR SF3A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16958">
        <property type="entry name" value="PRP9_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16837">
        <property type="entry name" value="SF3A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11931">
        <property type="entry name" value="SF3a60_Prp9_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12171">
        <property type="entry name" value="zf-C2H2_jaz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50171">
        <property type="entry name" value="ZF_MATRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Matrin-type" evidence="8">
        <location>
            <begin position="410"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="356"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="363"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR22172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="506" mass="58986" checksum="6632981779D9B149" modified="2009-07-28" version="1">MKTYEDARATLEELELIENAVTSRLRRNPEIFYRNIDEVDLVEHSGRFDHIPSSQKSANKVYTVKKSRRSRKQILAQEHEIKSFLSQYLKDCQSLGRVEFDMSEYRVDNGSVNKLLEHIERIKEIHPKDDEFGDQYAMFSSSTPSNKNIISRKAQNLDINSYFTRDEQYGEYLDLEPFHQKWLSVVKNGDVTLLQFLSEVEKFTDQRYLLQPAVNRNSSRYREFVASLHHYLSDYAARAYPLLSWDLIHAKAQELYHTNVAKPIPSGESGVFCIPCDKRFKTDTVYNAHLPGKRHVGNLHKNSSFLTQEHSLHIFCRYLSRELARTREFVERKLAFTTEERTQELERLTDIYDSPVYGRQEQEDAQEDENKETEDKEDSVLDSSMNLPLGPDGFPIPYWLYKLQGLDIEYPCELCGNFVYNGRRQFDKHFGEQRHVFGLRRLGIEPSASFKGVTKIDDAKQLWSHLRSTSSGRVSSEGKLEVEVEDEDGNVMSKKVYDELKKQGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEJ8</accession>
    <name>C5DEJ8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="7">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0C09856g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="7"/>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552647.1">
        <property type="nucleotide sequence ID" value="XM_002552601.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEJ8"/>
    <dbReference type="FunCoup" id="C5DEJ8">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEJ8"/>
    <dbReference type="GeneID" id="8291527"/>
    <dbReference type="KEGG" id="lth:KLTH0C09856g"/>
    <dbReference type="eggNOG" id="KOG2108">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004585_7_0_1"/>
    <dbReference type="InParanoid" id="C5DEJ8"/>
    <dbReference type="OMA" id="ENGWRGL"/>
    <dbReference type="OrthoDB" id="1470711at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000725">
        <property type="term" value="P:recombinational repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.486.10">
        <property type="entry name" value="PCRA, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014017">
        <property type="entry name" value="DNA_helicase_UvrD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000212">
        <property type="entry name" value="DNA_helicase_UvrD/REP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014016">
        <property type="entry name" value="UvrD-like_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070:SF46">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE HMI1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070">
        <property type="entry name" value="UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00580">
        <property type="entry name" value="UvrD-helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13361">
        <property type="entry name" value="UvrD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51198">
        <property type="entry name" value="UVRD_HELICASE_ATP_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51217">
        <property type="entry name" value="UVRD_HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="1 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UvrD-like helicase ATP-binding" evidence="10">
        <location>
            <begin position="5"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="UvrD-like helicase C-terminal" evidence="11">
        <location>
            <begin position="296"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="26"/>
            <end position="33"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR22209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="713" mass="79781" checksum="74A7448F4422C112" modified="2009-07-28" version="1">MPTPTPSQNEVIQCAYEPATTVKVIAGPGSGKTLTLMLKVRELISKGIVGADEVLVLSLTNKAVDNVINQLNSIFSELETTTDGIQGDVLTDLVAQIGVYTIHGLANRVVVENEGLVNIIEENGWRGLSKLIPANLLKKYVSGRRGTTLTPKMFERLFREYQAKGTVNEKDAAMEQIVTIMKASKVFTNEELISLATKYLRSGAANQASELPSMTNGLLHKYKVVIVDEYQDLFPSLGPFLSAISQNKQLFLFGDPHQSIYGFLGDNRSVIRFLESMRPPENLKVFHLYDNFRSTPEITRWANAVAGDVSRAVTNNNAFFSKEPMGIEPSLVDIADPLEELEIITDKICQLVSNSVKLSDIAVLTRTNAQLKAVGDHFSSYGIPTEKLTAQPDWISDKNIKFLVDLLKTCMLVSREKRFVEGAGQHQSDFSVIVTLSSLKGVSNQSIQTLFGEAQKANISLWNFISQSRQWPTGVSNRTRIESFVRHLEPLLEYLESSQPLTAISIISRLVTVSHQIGFKPDEIKNENDLEIFREHLIEMLGVLKLCSSTRPQNVSLTEWFLETWMDQHSSFIQHAANERSNIHDLGAVKISTIHSSKGLEFPVVFLSGSSNSNFPIEKNALYVGMTRARNLLYMMNVKNKFLPPPNPESGTGIFDNQKIWNYYNKDLNRPIGRQLESSLQKYRAVNQKFSLNPSVRAYSTMVRMVCNSLKHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEM9</accession>
    <name>C5DEM9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0C10560p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0C10560g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552678.1">
        <property type="nucleotide sequence ID" value="XM_002552632.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEM9"/>
    <dbReference type="FunCoup" id="C5DEM9">
        <property type="interactions" value="52"/>
    </dbReference>
    <dbReference type="GeneID" id="8291559"/>
    <dbReference type="KEGG" id="lth:KLTH0C10560g"/>
    <dbReference type="eggNOG" id="KOG0254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_27_4_1"/>
    <dbReference type="InParanoid" id="C5DEM9"/>
    <dbReference type="OMA" id="LCMSIAP"/>
    <dbReference type="OrthoDB" id="2130629at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17476">
        <property type="entry name" value="MFS_Amf1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000285">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000294">
        <property type="entry name" value="MFS transporter of unkown specificity"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718:SF1">
        <property type="entry name" value="LOW AFFINITY AMMONIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY MULTIDRUG TRANSPORTER MFSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="85"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="146"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="176"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="206"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="246"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="275"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="313"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="345"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="376"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="401"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="438"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="478"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="42"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR22240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="515" mass="55767" checksum="4E4911F51A033DB2" modified="2009-07-28" version="1">MSFTDKEENQKKDDGLTRESTHDQGQELSQPHHNGIQKPESLLGEAAFVGVICGAQLMTQAGLGQSIAPLHIIGDSFGTRNPGQLSWFAAAYSLTVGTFILIAGRLGDVYGHKKFFIIGFFWYALWSLLAGFSVYSNQIFFDCCRAFQGIGPALLLPNAIAILGRTYVPGRRKAMILSLFGATAPAGFVLGAVFSSIFGQLTWWPWAYWAMGIVCFFLAVAGILVIPRMPLPELDTSVSTYQRLDIPGSVTGVIGLVLLNFAWNQGPVVGWQTPYTYSLLIVSAVFLCVFAFIESRAPHPLLPLSALSSDAAFVLGCVGAGWSSFGIWIYYFWLFLEELRGHSPLLTSAQFAPVAISGLCAALTTGFVITRVRASLVMLFAMTAFTVGGILIATAPVKQVYWAQAFVSIIVMPWGMDMSFPAATIMLSDSMPHEHQGLAASLVNVVVNYSISIGLGLAGTVEGRVNNGGANLLKGYRGAWYMGIGLSGFGILIAFMYEYHTFKKSKKMPSEKPFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>C5DET8</accession>
    <name>C5DET8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">rRNA methyltransferase 1, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0D09680g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553131.1">
        <property type="nucleotide sequence ID" value="XM_002553085.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DET8"/>
    <dbReference type="FunCoup" id="C5DET8">
        <property type="interactions" value="301"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DET8"/>
    <dbReference type="GeneID" id="8295369"/>
    <dbReference type="KEGG" id="lth:KLTH0D09680g"/>
    <dbReference type="eggNOG" id="KOG0838">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021322_5_1_1"/>
    <dbReference type="InParanoid" id="C5DET8"/>
    <dbReference type="OMA" id="RKYAHVH"/>
    <dbReference type="OrthoDB" id="270651at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016435">
        <property type="term" value="F:rRNA (guanine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18105">
        <property type="entry name" value="SpoU-like_MRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1280.10:FF:000036">
        <property type="entry name" value="MRM1p Ribose methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047182">
        <property type="entry name" value="MRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047261">
        <property type="entry name" value="MRM1_MeTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004441">
        <property type="entry name" value="rRNA_MeTrfase_TrmH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013123">
        <property type="entry name" value="SpoU_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00186">
        <property type="entry name" value="rRNA_methyl_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46103">
        <property type="entry name" value="RRNA METHYLTRANSFERASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46103:SF1">
        <property type="entry name" value="RRNA METHYLTRANSFERASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08032">
        <property type="entry name" value="SpoU_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00967">
        <property type="entry name" value="SpoU_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="RNA 2-O ribose methyltransferase substrate binding" evidence="11">
        <location>
            <begin position="117"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="70"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="27"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="84"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR22693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="393" mass="44039" checksum="1E5CCBC1F8EB8590" modified="2009-07-28" version="1">MFKPRPNTILRRSLAQAIRPAVKVPNASHQGSGPSNIDKNLPHERRTKAWEKAGEDKDSWFRRKYAHVHAKAGNPNRQKGRAKQNVEEASVQHDQHKSKFRNRSVTGSLRPNPLMEYVHGTNSVISVLQADKRDYYSRILYHASVTDEIRSLAKKRGAQLEMTDKHRLNILTNYAVHNNVVLETKPLQLPEISQVGSCDPEGSTFAYDELFFDEASERRTSFIQKENKKFPLGLYLDEVSDPHNMGAIIRSAYFLGVDFMIMSRRNCAPLSPTVSKTSSGATELLPIFTVDKPLDFFSKSQKEGWTFVSAGVSKQNKFAKNKKIEAQDLHGMLKELPVILVVGNEGEGVRTNLQMRSDFLVEIPQGRGAAGTRSVDSLNVSVATAILLNSILN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEX5</accession>
    <name>C5DEX5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA-directed RNA polymerases I, II, and III subunit RPABC3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0D10538g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the eukaryotic RPB8 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553168.1">
        <property type="nucleotide sequence ID" value="XM_002553122.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEX5"/>
    <dbReference type="FunCoup" id="C5DEX5">
        <property type="interactions" value="877"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEX5"/>
    <dbReference type="GeneID" id="8295407"/>
    <dbReference type="KEGG" id="lth:KLTH0D10538g"/>
    <dbReference type="eggNOG" id="KOG3400">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103864_1_0_1"/>
    <dbReference type="InParanoid" id="C5DEX5"/>
    <dbReference type="OMA" id="KEDDKGW"/>
    <dbReference type="OrthoDB" id="20018at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000191">
        <property type="entry name" value="DNA-directed RNA polymerases I, II, and III subunit RPABC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005570">
        <property type="entry name" value="RPABC3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10917">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10917:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03870">
        <property type="entry name" value="RNA_pol_Rpb8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000779">
        <property type="entry name" value="RNA_pol_Rpb8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00658">
        <property type="entry name" value="RPOL8c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR000779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR22730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="144" mass="16312" checksum="6AC51087FA818FD3" modified="2009-07-28" version="1">MSTTLFDDIFQVTEVDLGRYNKVSRIEAHSTSQEFLLTVDINTELFPVRVNDTLTVMLSSTLSIDGSETVQGGSWRAPQPGERSLADDYDYVMYGTAYRFEEVAGKKDLIAVYYSFGGLLMRLEGNYRGLSNLKQENAYLLVRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DF49</accession>
    <name>C5DF49_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">V-type proton ATPase subunit a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0D12210g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Essential component of the vacuolar proton pump (V-ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the V-ATPase 116 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553242.1">
        <property type="nucleotide sequence ID" value="XM_002553196.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DF49"/>
    <dbReference type="FunCoup" id="C5DF49">
        <property type="interactions" value="448"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DF49"/>
    <dbReference type="GeneID" id="8295482"/>
    <dbReference type="KEGG" id="lth:KLTH0D12210g"/>
    <dbReference type="eggNOG" id="KOG2189">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005230_0_2_1"/>
    <dbReference type="InParanoid" id="C5DF49"/>
    <dbReference type="OMA" id="FYLWFFL"/>
    <dbReference type="OrthoDB" id="10264220at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000220">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051117">
        <property type="term" value="F:ATPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002490">
        <property type="entry name" value="V-ATPase_116kDa_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026028">
        <property type="entry name" value="V-type_ATPase_116kDa_su_euka"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629:SF63">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629">
        <property type="entry name" value="VACUOLAR PROTON ATPASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01496">
        <property type="entry name" value="V_ATPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001293">
        <property type="entry name" value="ATP6V0A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="412"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="457"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="534"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="568"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="628"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="725"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="754"/>
            <end position="777"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR22804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="820" mass="93710" checksum="F1F43FE0CC57CD66" modified="2009-07-28" version="1">MSEKYEAMFRSAEMSLVQLYVPQEIARDTVYSLGNRGLVQFRDLNRKVRAFQRTFVPEVRRLDELQRQYRYFYSLLCKYDIKLYEDFEDEEDEEVLAASPVASAHKLAPRTSKIDDHIENGTLIEKRMQQLVEASEQLELQKSDLEQFRHVLKAGDEFFAQSAGLTSQDSGEENFPRSVSFVTGTIPRAKAGTLEQILWRVLRGNLFFRTVPITEPLYDAKAQKKIYKDAFIVFSYGDIILQRVKKVAESLDASLYEISDDQSNRSKQLSEVNSRLADVYTVLDTTNTTLETELFAVSKELHAWNTEIAREKAVYETLNLFDFDSNRKTLVGEGWVPQDELGSLQVQLANLTETLGVDVPSIVQVLETNRTPPTFHRTNKFTAAFQNICDCYGTATYREVNPGLPTIVTFPFMFAVMFGDMGHGMLMSMVAGLLVFNEKALGKMKRDEIFDMAFSGRYILLLMGLFSIYTGFLYNDMFSKSLTLFKSGWEWPSHWHLGEAIEAKSVGTYVFGLDWAWHGTENALLFSNSYKMKLSVLLGFIHMTYSYMFSLVNYVYFQKWIDIIGNFIPGLLFMQGIFGYLTVCIIYKWTIDWVAIGKPAPGLLNTLINMFLSPGTVEEELYPHQAKVQVFLLIMALICIPCLLLIKPLHFKFTQDKHQHIALPDNEAGLVHHETNTGAGAVEDDDDDEEGGHGEEFGDVMIHQVIHTIEFCLNTVSHTASYLRLWALSLAHAQLSTVLWTMTIQIAFSMRGFVGVVMTVILFGMWFVLTCVILVLMEGTSAMLHSLRLHWVESMSKFFEGEGYPYEPFKFMPVEFENEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DF76</accession>
    <name>C5DF76_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0D12826p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0D12826g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553269.1">
        <property type="nucleotide sequence ID" value="XM_002553223.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DF76"/>
    <dbReference type="FunCoup" id="C5DF76">
        <property type="interactions" value="169"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DF76"/>
    <dbReference type="GeneID" id="8295509"/>
    <dbReference type="KEGG" id="lth:KLTH0D12826g"/>
    <dbReference type="eggNOG" id="KOG0235">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033323_9_2_1"/>
    <dbReference type="InParanoid" id="C5DF76"/>
    <dbReference type="OMA" id="NYPNMET"/>
    <dbReference type="OrthoDB" id="354304at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004331">
        <property type="term" value="F:fructose-2,6-bisphosphate 2-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045820">
        <property type="term" value="P:negative regulation of glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043456">
        <property type="term" value="P:regulation of pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1240:FF:000051">
        <property type="entry name" value="Phosphoglycerate mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001345">
        <property type="entry name" value="PG/BPGM_mutase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051695">
        <property type="entry name" value="Phosphoglycerate_Mutase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46517">
        <property type="entry name" value="FRUCTOSE-2,6-BISPHOSPHATASE TIGAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46517:SF1">
        <property type="entry name" value="FRUCTOSE-2,6-BISPHOSPHATASE TIGAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00175">
        <property type="entry name" value="PG_MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="7">
        <location>
            <position position="24"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="7">
        <location>
            <position position="104"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="23"/>
            <end position="30"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR22831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="230" mass="26139" checksum="1575267EFDB734D2" modified="2009-07-28" version="1">MTKDASYYTANEDPGVLRIFVIRHGQTAENSKKILQGHLDTDLNETGIEQAHKLGSYLANDKKIKFDKIYTSDLKRCQQTLAQILDSYPKQDTPEVVKDSGLRERSMGEIQGMHLDQAEEYAAKHGKGSFRDFGEKPDAFGKRLTTAVNNIVEDCRDLKNIAMVSHGGSIRQLLMWLDYQEHDIRRIVVFNTSVTVIDYVKDAKRYDVKKVGMTQHLGSGEFVVSDLRLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFS0</accession>
    <name>C5DFS0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0D17424p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0D17424g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HIR3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR23025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553463.1">
        <property type="nucleotide sequence ID" value="XM_002553417.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DFS0">
        <property type="interactions" value="187"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFS0"/>
    <dbReference type="GeneID" id="8295713"/>
    <dbReference type="KEGG" id="lth:KLTH0D17424g"/>
    <dbReference type="eggNOG" id="ENOG502QQX4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001316_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFS0"/>
    <dbReference type="OMA" id="WETWYRL"/>
    <dbReference type="OrthoDB" id="77564at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000417">
        <property type="term" value="C:HIR complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033053">
        <property type="entry name" value="Hir3/CABIN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15502">
        <property type="entry name" value="CALCINEURIN-BINDING PROTEIN CABIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15502:SF7">
        <property type="entry name" value="CALCINEURIN-BINDING PROTEIN CABIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="305"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1584"/>
            <end position="1622"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="578"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1586"/>
            <end position="1596"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR23025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1639" mass="187125" checksum="49EC7016EF4E5E2B" modified="2009-07-28" version="1">MSLFTALNFTPDDALQEAEQHSRELQVEESSKIYQSALLHLKAKRFEQAEEKFKELFNIDVIKPDKWGLYKYSSPTLNSLRYLAYRNRGVFYYQFLQEKHENMSSQDIVEYTLKVAENLLDAIQHSNCDAAVAKLLISIFKSFRSVKLQRYTLEYELLKECGSTTALKRKKTVLPEVRDFIHRYVELLRSSEDTGSLESDFIVHLKCLLKSSSPLSLELPPFLSRIQTMKLEDDLALREENEVSVVVNELSWEAVAEAVVSTLPKYKFSNFFSKISDPYNEAEEPIEKFKFVLNDLHNEVEVDVEEEHSEHGEQANLITEDDTKNVTAPEDTPGQEGINQKRPLDAAEVNKPLQRMSKRFKEKPQVASDARIMDNHEKFLQVINDVALSTGYDLILTTDKLAPQDVPKEAPYFNAISDLYDCISSWTNKHSEFLNQTDTKNSKSSSTDVESTLQLNALIRTSVLAGDNAPIESLTDLPADDIQSFINRINKDSYHFQEVRLMLLDSLLSTDTDGRCLIIDTFWSPVLFKTVEFLVMSVETNLFNMIGNAKSGLGSLGLSAYEILINMLGNIYSNISTKKLQGQKVNELETQKNKLERKISRWTLLLEELNFDDERSFYRFLWAKFCSLQYSTDITDGKLVLNLQEIESKIGKNGSRINVPYSNYSTIPHLNLKAVQSQLSRLKIIQKLTSIDVDVPQEDNDVATKQQMELIRKALVSDEYMNPETSSEEELSMESFLRNSPFLLKVKLWRLLVSFYSQRDKAIAQAVYFKILRILYERLCSDEYGAQSQLQRQQTLLLTISCIGSFTRGFVEMVQGIHSNSSKDNNATKTNLHTLFKILRILYPIVFFESLCQKDSNLKSFFRKAVKSSTKLKSVFTDTITSIIYMCSLLEREQTKISSLVEVAHEILGSFDFCDASEGSFLHFAEQLLCKQNNEGSFNQIKQLLWCQYHLLIGGDSSAIEQHNTKGQVMTQDSAVALGKFLINSHYGDRNPLSVSGNKTNLKQTLDSIIQVVGDVDISANYILSRNEFFLEQYLKSVITANTFRKAYAGKLSLQLVKPNDKLQGVADLGLFFVASIQALNLYKARKKATQARPSELDSVISTLKTDILYNTERFESWMLLGKCYSYIVEDDLIWTSDKLATSEKRKGTTSIQRKAILCYLMALGLAMRSKGKFPTSSQVSDYQDILKELFETLSEEILNGYLKPMEASCFQWEPPTSVKLTVEGELAAAQFYATQTVSDFNIQQAILTGFSKAIKIEASHLASKNRPKNWKNLYYTAKIIFKRCAEEYKTDGLNILLQACSVCDDATSSKELILEPHYFLTVSCFKLVKGGFMTPQQALLSLQKNNSIFQKDDDFWLLTSAEASEEARHNFCCKIVALLRALIAMDKMKWHHRPRYRIARILYEEMHDLDGAISEMDELMFLKSSNKSLVNIWKPKYERPGKHFIYTFQYVIFYLDLLFCRNDYSSIALVAKKLKRFGPGMVNVATAADRAMRLFCQSVQDTFGVNEKLALDKTLPSLNYQQFLRYSDLLFQSFDKTKYDSKVLDTLVHALQLKKGNNGYDGICLALYFTFIYLPWASQKEVQDKSQTSDPNMNNKAIEPTQEPPNGLVKGKASTNRKRVSKKDAFDKISALVEKRIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFV0</accession>
    <name>C5DFV0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0D18128p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0D18128g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M28 family. M28B subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR23055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553493.1">
        <property type="nucleotide sequence ID" value="XM_002553447.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFV0"/>
    <dbReference type="FunCoup" id="C5DFV0">
        <property type="interactions" value="148"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFV0"/>
    <dbReference type="GeneID" id="8295745"/>
    <dbReference type="KEGG" id="lth:KLTH0D18128g"/>
    <dbReference type="eggNOG" id="KOG2195">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005688_2_0_1"/>
    <dbReference type="InParanoid" id="C5DFV0"/>
    <dbReference type="OMA" id="QGSTEWV"/>
    <dbReference type="OrthoDB" id="5841748at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02121">
        <property type="entry name" value="PA_GCPII_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000101">
        <property type="entry name" value="N-acetylated alpha-linked acidic dipeptidase like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.40">
        <property type="entry name" value="Transferrin receptor-like, dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039373">
        <property type="entry name" value="Peptidase_M28B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007365">
        <property type="entry name" value="TFR-like_dimer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036757">
        <property type="entry name" value="TFR-like_dimer_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10404">
        <property type="entry name" value="N-ACETYLATED-ALPHA-LINKED ACIDIC DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10404:SF46">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04253">
        <property type="entry name" value="TFR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47672">
        <property type="entry name" value="Transferrin receptor-like dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="4">
        <location>
            <begin position="202"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M28" evidence="6">
        <location>
            <begin position="388"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="domain" description="Transferrin receptor-like dimerisation" evidence="5">
        <location>
            <begin position="659"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="303"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR23055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="773" mass="87303" checksum="DB124CD3AE1CC1C8" modified="2009-07-28" version="1">MEAHAESESQPLLHPTRTRENSVASLFARGRSNTINGLKTGYDTVKRHRIQFLYFSFASLVLYLTFTLAFLPRTSLSRDFRRLHFSKFTKAETYRIYLESLLRENQIERHLDAYTAERHLAGDQNALDYTVNELKALGFSPKIEKYYPWLNTPIDSGMSLWINGTLEFKSGMAEDALEEDSTSFSPFPTPAFHGYSANGNVTARFVYCNYGKNEDYEFLKNKGVHLEGMIHVIRYGVLFRGLKVKNAEKNGAAGVILYTDPYEDGQVTERNGFKSYPDGPARHASGFERGSVQFFTDFPGDPTTPGYASKSPETKRVSPGGKMPTIPSIPMSAKDISRILPYLNSRGCQFDVEGNIKGFEYYSGPSDEHTKLQVFNEQDYKVEEIFDVITEIPGILKGSSIIIGSHRDSWTTGGAADPGSGSSILLEVARGLSKLLEKGWKPLRTIKLISWDGEEPAMLGSTEYGENHAVKLQKQSLAYLNLDTVITGCIFRCEANPLLSEVLKEAASLTPFHGNPDYSLLEEWKNRTGGKVDVLGAGTDFMVFQNHLGIPSANFKFLSNEINDPVYQHHTIYDSLTWMKRFGDRNYESHNTMAIFTGISVLMLSENELVTFKTHDYAQLIQENFKKWHALLNKAFPNDQYIASESLKLSSLISLIADNASVKFDDNVTSLRKKVSQDFPWWKIYKKIAIFLRLSVANNKLKKVDRLFVTDRGLKDRGWMRHSIFAPEKESGYVGDVMPGLHEAIVAGDPQEILEWLHILTNQMSLIRELLEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFZ7</accession>
    <name>C5DFZ7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0D01210p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0D01210g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552777.1">
        <property type="nucleotide sequence ID" value="XM_002552731.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFZ7"/>
    <dbReference type="FunCoup" id="C5DFZ7">
        <property type="interactions" value="129"/>
    </dbReference>
    <dbReference type="GeneID" id="8294997"/>
    <dbReference type="KEGG" id="lth:KLTH0D01210g"/>
    <dbReference type="eggNOG" id="ENOG502RQJB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028816_1_0_1"/>
    <dbReference type="InParanoid" id="C5DFZ7"/>
    <dbReference type="OMA" id="RRMVYVY"/>
    <dbReference type="OrthoDB" id="4090479at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057511">
        <property type="entry name" value="WH_GDS1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25318">
        <property type="entry name" value="WHD_GDS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GDS1 winged helix" evidence="2">
        <location>
            <begin position="111"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="208"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="305"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="30"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="221"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="317"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR22339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="431" mass="46318" checksum="942CC175B46EEF38" modified="2009-07-28" version="1">MALTNSRPLQMPALENEALHNSASPVFQPSSLRSSARSSCSESTSEMDHALSTSDTSEGASDTRLTRKDVLALKRSATASPLTPTGAVSELSKLVPVTGQRPKPTKKDLFMDDDILLAIFVILYEKDPNQEGVTVKQLCDYLVDKHPDMAGASTKLSNLISAKLNAYVKKVEKGEKTLKYALSRQWSEGSPRRMVYVYRGILAPDYKSHTQSMSSQRQDAKQTGKSTTSSSKKAHGLSNNATDANMDYSLTESKVSNQAKGTFSINNNSFTINSFGSDFNIPYAASPVTMRMMPNLAPNAFQDDQLSKAARDRRPKTSLSGPPSKKPNLQASPVVPPYITAAAAAPRLSKGVPKRNAASGELLNAAASSIQKLTAFPKQGEASCITDIALVPLAPTTTCPSWLQTIQSGFLLEDIESPEAVSLEDLDGLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGD0</accession>
    <name>C5DGD0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0D04312p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0D04312g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
        <text evidence="8">The heme is bound between the two transmembrane subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552910.1">
        <property type="nucleotide sequence ID" value="XM_002552864.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGD0"/>
    <dbReference type="FunCoup" id="C5DGD0">
        <property type="interactions" value="434"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGD0"/>
    <dbReference type="GeneID" id="8295136"/>
    <dbReference type="KEGG" id="lth:KLTH0D04312g"/>
    <dbReference type="eggNOG" id="KOG0449">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094691_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGD0"/>
    <dbReference type="OMA" id="MNGIRHL"/>
    <dbReference type="OrthoDB" id="588261at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006121">
        <property type="term" value="P:mitochondrial electron transport, succinate to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03499">
        <property type="entry name" value="SQR_TypeC_SdhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1300.10">
        <property type="entry name" value="Fumarate reductase/succinate dehydrogenase, transmembrane subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034804">
        <property type="entry name" value="SQR/QFR_C/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018495">
        <property type="entry name" value="Succ_DH_cyt_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014314">
        <property type="entry name" value="Succ_DH_cytb556"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000701">
        <property type="entry name" value="SuccDH_FuR_B_TM-su"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02970">
        <property type="entry name" value="succ_dehyd_cytB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10978">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE CYTOCHROME B560 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10978:SF5">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE CYTOCHROME B560 SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01127">
        <property type="entry name" value="Sdh_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000178">
        <property type="entry name" value="SDH_cyt_b560"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81343">
        <property type="entry name" value="Fumarate reductase respiratory complex transmembrane subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01000">
        <property type="entry name" value="SDH_CYT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01001">
        <property type="entry name" value="SDH_CYT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 8" id="KW-0349">Heme</keyword>
    <keyword evidence="6 8" id="KW-0408">Iron</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="68"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="113"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="147"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="8">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <note>ligand shared with second transmembrane subunit</note>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000178-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR22472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="168" mass="18743" checksum="1B2E3D5472729EA2" modified="2009-07-28" version="1">MFSLGLNSGAFWARPLRTASRRMIATAKTSPARQQEILIAQRKNRPVSPHLTIYQPQLTWYLSSLHRVSGVILAFGFFATTIAFGTSALLGLGLTSNNLARWYHEKVPRWMDLTAKGSFAYLFAFHFANGLRHLIWDAGKALTLRGVYTTGYAVMAVMALGGTYLLTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGF8</accession>
    <name>C5DGF8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0D04972p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0D04972g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552938.1">
        <property type="nucleotide sequence ID" value="XM_002552892.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGF8"/>
    <dbReference type="FunCoup" id="C5DGF8">
        <property type="interactions" value="56"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGF8"/>
    <dbReference type="GeneID" id="8295165"/>
    <dbReference type="KEGG" id="lth:KLTH0D04972g"/>
    <dbReference type="eggNOG" id="ENOG502S2CK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104695_1_0_1"/>
    <dbReference type="InParanoid" id="C5DGF8"/>
    <dbReference type="OMA" id="EPNSGKC"/>
    <dbReference type="OrthoDB" id="5419752at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005786">
        <property type="term" value="C:signal recognition particle, endoplasmic reticulum targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039914">
        <property type="entry name" value="SRP9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039432">
        <property type="entry name" value="SRP9_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12834">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 9 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12834:SF12">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 9 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05486">
        <property type="entry name" value="SRP9-21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SRP9" evidence="2">
        <location>
            <begin position="4"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="110"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR22500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="132" mass="14241" checksum="74731874EDEB6579" modified="2009-07-28" version="1">MSVDALDSFITNSVTLLEANPSETVLSVTYNPKAKSGKVAFKTHNSKLSTRFKFTTSRSKDVSRLLSALGPRGVSINNGKIEKKRTGKKVQAVGGMSTLLANVDVPEALEEPIQQAKPSKPASGKKKKNKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGJ6</accession>
    <name>C5DGJ6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0D05830p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0D05830g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552976.1">
        <property type="nucleotide sequence ID" value="XM_002552930.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGJ6"/>
    <dbReference type="FunCoup" id="C5DGJ6">
        <property type="interactions" value="332"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGJ6"/>
    <dbReference type="GeneID" id="8295204"/>
    <dbReference type="KEGG" id="lth:KLTH0D05830g"/>
    <dbReference type="eggNOG" id="KOG1471">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014001_0_1_1"/>
    <dbReference type="InParanoid" id="C5DGJ6"/>
    <dbReference type="OMA" id="QVMFNDF"/>
    <dbReference type="OrthoDB" id="1434354at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.525.10:FF:000011">
        <property type="entry name" value="SEC14 cytosolic factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051026">
        <property type="entry name" value="PI/PC_transfer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45657">
        <property type="entry name" value="CRAL-TRIO DOMAIN-CONTAINING PROTEIN YKL091C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45657:SF1">
        <property type="entry name" value="CRAL-TRIO DOMAIN-CONTAINING PROTEIN YKL091C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="1">
        <location>
            <begin position="25"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="229" mass="26457" checksum="619385439A03AE95" modified="2009-07-28" version="1">MYENCEKWRKDFGVDTIFEDFHYDEKPLVAKYYPQYYHKTDKEGRPLYFEELGSVNLTEMYKITNQERMLRNLVWEYESFVRYRLPACSRQAGYLVETSCTILDLKGISISAAAQVLSYVKEASNIGQNYYPERMGKFYLINAPFGFSTAFRLFKPFLDPVTVSKIFILGSSYQKELLKQIPAENLPVKFGGKSEVDESQGGLYLSDVGPWRNPKFIGPEGEAPTAFKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGM0</accession>
    <name>C5DGM0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0D06424p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0D06424g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553000.1">
        <property type="nucleotide sequence ID" value="XM_002552954.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGM0"/>
    <dbReference type="FunCoup" id="C5DGM0">
        <property type="interactions" value="20"/>
    </dbReference>
    <dbReference type="GeneID" id="8295231"/>
    <dbReference type="KEGG" id="lth:KLTH0D06424g"/>
    <dbReference type="eggNOG" id="ENOG502RXS6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062497_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGM0"/>
    <dbReference type="OMA" id="NYYCWNT"/>
    <dbReference type="OrthoDB" id="5358702at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005968">
        <property type="term" value="C:Rab-protein geranylgeranyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004663">
        <property type="term" value="F:Rab geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.120">
        <property type="entry name" value="Protein prenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11129:SF8">
        <property type="entry name" value="PROTEIN ECM9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11129">
        <property type="entry name" value="PROTEIN FARNESYLTRANSFERASE ALPHA SUBUNIT/RAB GERANYLGERANYL TRANSFERASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48439">
        <property type="entry name" value="Protein prenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR22562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="381" mass="44243" checksum="9D281E85E9F760D7" modified="2009-07-28" version="1">MKNSNLSKRLYDCLTDDYNNKQIRLVVLPDYFREGDSDGIVCVSDGNAIEIICFRTFYLQVYSEARKVLNDRQSSQAAANEERYYLATTVLLLITPEDHSNIRRHEELLLNRLKDKNCSGLWRRDEIAGAFVRRELQFVTSCLTSSLARVNKSPSLWLLYRKLYIITNSLFPTIEANYEMCFSKSAEAHSSNYYSWHTLRWMFDVGSKQLKARLAEATETFCFKHPKDSSAWWSLAHVLVSSFDTQKNSIAQQNMLTANYSLPPLSELIFGCASSTEIESKVKKIVEYIDYAEVSEYPPFQCLEKLFQNLNQTGQKAYVEKWLSQVEVFERENFEIGESSALLSDRQLRENVLIQRDFKTLVMKKRFVQRLLPHNTPKSTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGM1</accession>
    <name>C5DGM1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein mL50</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0D06446g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mL50 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553001.1">
        <property type="nucleotide sequence ID" value="XM_002552955.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGM1"/>
    <dbReference type="FunCoup" id="C5DGM1">
        <property type="interactions" value="353"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGM1"/>
    <dbReference type="GeneID" id="8295232"/>
    <dbReference type="KEGG" id="lth:KLTH0D06446g"/>
    <dbReference type="eggNOG" id="ENOG502S1NZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103468_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGM1"/>
    <dbReference type="OMA" id="FQFTKFL"/>
    <dbReference type="OrthoDB" id="3980895at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018305">
        <property type="entry name" value="Ribosomal_m50"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10501">
        <property type="entry name" value="Ribosomal_L50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR22563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="249" mass="28113" checksum="A6EE1881AFA2D8DF" modified="2009-07-28" version="1">MLSNRIASCSVKRGLHVSASSKDFMSWFRRKQTAKPAAPIKKTEELINEVESGVGGAEAPLNRLKLTDEFFIGKSNLDLDRRERQSRVNEVPFNKWMSSEKLKSSGVLDSILLESYNSTNSEFRAIAIADEKLALPFTDLVFKFHFSKALQSATGTLIPDYQITRLQTPIAFRNYLLKEVVSGKLAKFRESEPNAIELGQGHYSSDSIHVVEDVRFSERKKKLSKILSEVEFLEREAARQAVEKARQAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGR2</accession>
    <name>C5DGR2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0D07414p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0D07414g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553042.1">
        <property type="nucleotide sequence ID" value="XM_002552996.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGR2"/>
    <dbReference type="FunCoup" id="C5DGR2">
        <property type="interactions" value="885"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGR2"/>
    <dbReference type="GeneID" id="8295274"/>
    <dbReference type="KEGG" id="lth:KLTH0D07414g"/>
    <dbReference type="eggNOG" id="KOG2348">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010778_2_1_1"/>
    <dbReference type="InParanoid" id="C5DGR2"/>
    <dbReference type="OMA" id="LGANWLT"/>
    <dbReference type="OrthoDB" id="6132759at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015489">
        <property type="term" value="F:putrescine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015606">
        <property type="term" value="F:spermidine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015204">
        <property type="term" value="F:urea transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11476">
        <property type="entry name" value="SLC5sbd_DUR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1730.10:FF:000006">
        <property type="entry name" value="Urea active transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031155">
        <property type="entry name" value="DUR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00813">
        <property type="entry name" value="sss"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46154">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46154:SF4">
        <property type="entry name" value="UREA ACTIVE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="28"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="72"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="148"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="181"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="213"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="273"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="305"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="373"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="416"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="445"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="472"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="513"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="626"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="660"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="558"/>
            <end position="583"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR22604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="744" mass="80401" checksum="0D0D47C35C094888" modified="2009-07-28" version="1">MPSSLEGSPSIETFKQEMALIESPLPQGAGYAVVVGLGFVFAFGMILTTYMLKRYQKEIITAEEFATAGRTVKTGLIAAAVVSSWTWAATLLQSTAMVYKVGISGGYYYGAGACFQIIAFAALAIKAKQRAPNAHTFLEIIYARYGKVAHICYLFYALATNILVTAMLLTGGSAVVNDLTGMNTVAACFLLPLGVIVYTLFGGIKATFLTDYVHTIVIIVIILTFAFTVYATGDKLGSPGKVYDLVRAAAQAHPIEGNAGGEYLTMRSKSGGIFFVINLVGNFGTVFLDNGYWNKAISASPAASLPGYVLGGVSWFAIPTLISTAMGLACVALESNPSFPTYPQRLTSDQVSAGLVLPSAAVTILGKGGAVAALLMVFMAVTSAMSAELIAVSSIFTYDIYRTYINPNATGKKLIFSSHAACIIFGFSMAGFAVGLYYAGVSMGYLYELMGIIISAAVLPACLTLFWKQQNLAAVVISPILGTGLAIMAWLVCAKSQFNEITIDSTFADDPMLTGNVVALLSPMVFIPILTYAFKPQKFNWDLLQVITRADETEEIEAAEGQTEGENGQVSVRGDTSDSGNSIEANDREKLAAVKSVTSAAQEVPETQIDAMKREEAERLEVAGKWGTYLCIFFALALLVVWPMPMYGSKYIFSKKFFRGWVVVLIIWLFLSGFCVCIYPLWEGREGIYTTCRGIYWDLTGQTYKLREWQNSHPEKLHVVQSQISARAHASRRSLDGNIDDIVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DH07</accession>
    <name>C5DH07_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0E00352p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0E00352g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553505.1">
        <property type="nucleotide sequence ID" value="XM_002553459.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DH07"/>
    <dbReference type="STRING" id="559295.C5DH07"/>
    <dbReference type="GeneID" id="8291640"/>
    <dbReference type="KEGG" id="lth:KLTH0E00352g"/>
    <dbReference type="eggNOG" id="ENOG502QTSE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015811_0_0_1"/>
    <dbReference type="InParanoid" id="C5DH07"/>
    <dbReference type="OrthoDB" id="2428527at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051615">
        <property type="entry name" value="Transcr_Regulatory_Elem"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31313:SF82">
        <property type="entry name" value="ACTIVATORY PROTEIN CHA4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31313">
        <property type="entry name" value="TY1 ENHANCER ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Xylanolytic transcriptional activator regulatory" evidence="7">
        <location>
            <begin position="211"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR23068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="515" mass="58354" checksum="29D6DE8EFABE2538" modified="2009-07-28" version="1">MKAAKDIREIKKILQSLPINDVNQDIPEGAICSNSGNSTGLVAPKLGNMVYSSIYPSDSLNISKEITTSNAEEALPGFLSAKARNLSKDPYILSAMSNFFRWLYPGHYMFIHRETLLKALFEKPTTKSYYCSEELVLAVASLGSQIAESQDELLQKADEFYATSKSLVLGKIFQANQHTSGYSKNSSKLAIIQTLLCLAFYDIGRGENPMAWYFSGLAFRIAHEVGLHLNPKTWNNVYASDLSRIDVEVRSRIYWGCYIADHLICTLFGRSKSLRLSNSTTPETEDLPDIEAGIEMFQYDPAASLSVVKPLKSLIALSKVAELFVRKIFGPCSSLTQRDESLNKLNMSFHEWRCNLPSSLKWSKTCLKTFDFDPTKAFVWIHFYVVSLSYNKPFINDVESCRMVVKECIEDLYYLFSSFKRKNETLCKSSLYLCYSAILAVQCIKFGTIQQEYLEYFMNFLKSPSQPYDLGRKILELELDTESFDVFGFFASGDDYSSEFDFEFVLSNCGIQSPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DH16</accession>
    <name>C5DH16_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0E00616p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0E00616g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; biotin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553514.1">
        <property type="nucleotide sequence ID" value="XM_002553468.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DH16"/>
    <dbReference type="FunCoup" id="C5DH16">
        <property type="interactions" value="254"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DH16"/>
    <dbReference type="GeneID" id="8291652"/>
    <dbReference type="KEGG" id="lth:KLTH0E00616g"/>
    <dbReference type="eggNOG" id="KOG1401">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016922_4_3_1"/>
    <dbReference type="InParanoid" id="C5DH16"/>
    <dbReference type="OMA" id="GGCFMHG"/>
    <dbReference type="OrthoDB" id="425114at2759"/>
    <dbReference type="UniPathway" id="UPA00078"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004015">
        <property type="term" value="F:adenosylmethionine-8-amino-7-oxononanoate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004141">
        <property type="term" value="F:dethiobiotin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00834">
        <property type="entry name" value="BioA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005815">
        <property type="entry name" value="BioA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00508">
        <property type="entry name" value="bioA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42684">
        <property type="entry name" value="ADENOSYLMETHIONINE-8-AMINO-7-OXONONANOATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42684:SF3">
        <property type="entry name" value="ADENOSYLMETHIONINE-8-AMINO-7-OXONONANOATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0093">Biotin biosynthesis</keyword>
    <keyword evidence="7 8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR23077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="492" mass="55498" checksum="996D27CD3DE0F673" modified="2009-07-28" version="1">MSDLSFTPEVEKILDFDRKHLWHPYTSMSNPLKVYPVKSAAGATITLDTKGPGEATLVEAMSSWWCMIHGYNNPEINGAIIEQIGRMSHVMFGGLTHKPAVGTAEKLLRLIDHEKLNYCFLADSGSVAVEVAMKMALQYEFTKNGDKTRKSKFLTIRNGYHGDTFGAMSVCDPVNSMHSLYSGYLPQNIFVTAPSVIDTLPTSNFLRSNPEVFEGKVDWNERDIIEFEEIVRARHAEICAVILEPILQGAGGMRLYHPQYLIEVRKLCTKYKVPLIMDEIATGFCRTGEMFAFHHCRKYQKMLNIQPENHIDVYPDILCVGKALTGGYLTLSAVVTTAEIKDVISRRDSVTGGCFMHGPTFMGNPLACSAAEKSLEILLRGECYNQVASIEAQLMKQMYLPLLNDLTLMSTVIKHMRVIGAVGVIELRQVVDSAWFHEKFISKRVYVRPFGKLLYIMPPYVISEAELSKVIEAINQILREWKFHLENKKLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>C5DH68</accession>
    <name>C5DH68_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0E01782p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0E01782g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553566.1">
        <property type="nucleotide sequence ID" value="XM_002553520.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DH68"/>
    <dbReference type="STRING" id="559295.C5DH68"/>
    <dbReference type="GeneID" id="8291705"/>
    <dbReference type="KEGG" id="lth:KLTH0E01782g"/>
    <dbReference type="eggNOG" id="ENOG502SDCM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1326585_0_0_1"/>
    <dbReference type="InParanoid" id="C5DH68"/>
    <dbReference type="OMA" id="TMLTTHE"/>
    <dbReference type="OrthoDB" id="10435142at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037738">
        <property type="entry name" value="Ecm13-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36826">
        <property type="entry name" value="PROTEIN ECM13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36826:SF1">
        <property type="entry name" value="PROTEIN ECM13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="207" mass="22887" checksum="471AD94EB118324A" modified="2009-07-28" version="1">MNQTPLLMSVSRQCLLAQKVRQKLIRCASPAKDTDIDLRLLVGHANLLDRLAREAADAAAAAVPYEATEQRTKSRHHVPKSSQSVLELENSDSESDSDADSDADADEFWSSSDSDDDADSDDFYSESDGDSDLDPEEEADAEWDTQMGIAKYEAAGPYCLYKPVSRYITTTTVVLSDEEDEDDEEPVCHDHTALRRFPAVEPALIRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHF8</accession>
    <name>C5DHF8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Large ribosomal subunit protein mL38</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0E04004g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the phosphatidylethanolamine-binding protein family. Mitochondrion-specific ribosomal protein mL38 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553656.1">
        <property type="nucleotide sequence ID" value="XM_002553610.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHF8"/>
    <dbReference type="FunCoup" id="C5DHF8">
        <property type="interactions" value="272"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHF8"/>
    <dbReference type="GeneID" id="8291805"/>
    <dbReference type="KEGG" id="lth:KLTH0E04004g"/>
    <dbReference type="eggNOG" id="KOG3346">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068504_0_0_1"/>
    <dbReference type="InParanoid" id="C5DHF8"/>
    <dbReference type="OMA" id="FRTQWDE"/>
    <dbReference type="OrthoDB" id="2153661at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00866">
        <property type="entry name" value="PEBP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.280.10:FF:000004">
        <property type="entry name" value="Mitochondrial large ribosomal subunit YmL35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.280.10">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008914">
        <property type="entry name" value="PEBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036610">
        <property type="entry name" value="PEBP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035810">
        <property type="entry name" value="PEBP_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362:SF82">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE-BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01161">
        <property type="entry name" value="PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49777">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00037226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00039444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR23219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="357" mass="40820" checksum="8E8CC55A5F8C0CF8" modified="2009-07-28" version="1">MLRRSLHSSVVKRSGSKIWSEFTSRPEALSIGSERIKKCVLEGTPSQGPPSIKRRSNRVKYSSPEKIDEVFKTCYDFLESRSAVKYAELEREEDPAKRTKLLVEAEVNNPEVLYNFQYGDKVENNPRFIDYNVPVYRHLGRQHWESYGQMLLMQRLETLAAIPDTLPTLVPRAEVHLRFPFSTGLNKWVEPGELLSSNATTLPPAIKIQEYDDVDTESQEYTVLILNPDEPDIASDSFKTTLQYGLTNLKISYNDNVVDSRKFTADNIIAGYLPPVPEKNAGVQRFVVWVFRQSKHLAAGEAASARDNFNVRDFVHAHKLQPIGAHLWRSEWDSNVTNVRAKYGLPEGRVFHRVRKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHG0</accession>
    <name>C5DHG0_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Mannan endo-1,6-alpha-mannosidase</fullName>
            <ecNumber evidence="4 14">3.2.1.101</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">KLTH0E04048g</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Required for normal synthesis of the cell wall.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>Random hydrolysis of (1-&gt;6)-alpha-D-mannosidic linkages in unbranched (1-&gt;6)-mannans.</text>
            <dbReference type="EC" id="3.2.1.101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the glycosyl hydrolase 76 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.101" evidence="4 14"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553658.1">
        <property type="nucleotide sequence ID" value="XM_002553612.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHG0"/>
    <dbReference type="FunCoup" id="C5DHG0">
        <property type="interactions" value="37"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHG0"/>
    <dbReference type="GeneID" id="8291807"/>
    <dbReference type="KEGG" id="lth:KLTH0E04048g"/>
    <dbReference type="eggNOG" id="ENOG502QWHG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025694_1_2_1"/>
    <dbReference type="InParanoid" id="C5DHG0"/>
    <dbReference type="OMA" id="GMFQPPY"/>
    <dbReference type="OrthoDB" id="4187847at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008496">
        <property type="term" value="F:mannan endo-1,6-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007117">
        <property type="term" value="P:budding cell bud growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016052">
        <property type="term" value="P:carbohydrate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009272">
        <property type="term" value="P:fungal-type cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000006">
        <property type="entry name" value="Mannan endo-1,6-alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005198">
        <property type="entry name" value="Glyco_hydro_76"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014480">
        <property type="entry name" value="Mannan-1_6-alpha_mannosidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12145">
        <property type="entry name" value="MANNAN ENDO-1,6-ALPHA-MANNOSIDASE DCW1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12145:SF21">
        <property type="entry name" value="MANNAN ENDO-1,6-ALPHA-MANNOSIDASE DFG5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03663">
        <property type="entry name" value="Glyco_hydro_76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016302">
        <property type="entry name" value="Man_a_manosd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 14" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="7 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 16" id="KW-0732">Signal</keyword>
    <keyword evidence="15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002948738" description="Mannan endo-1,6-alpha-mannosidase" evidence="16">
        <location>
            <begin position="19"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="432"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00054068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR016302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAR23221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="454" mass="49920" checksum="F1BC986F5FDF2842" modified="2009-07-28" version="1">MLAQSFALCALLLQPILGIDLDTSDSSSICDATSLIQGGIMDYYAGTRIGGTVGMFQQPYYWWEAGEVFGGMLENWFLCQNDTYHDIIYDAMIHQAGPDYDFMPSNQSMVEGNDDQGVWGLTVMSAAERNFTNPTEDGVPGWLAMAQGVFNTMWMRWDSEHCGGGVRWQIFTWNSGYNYKNTVSNACMFQLAARLGRYTGNDTYLTKAETVWDWLVDVGFVVLNENGNANVYDGANIDDNCTDIVKNEWSYNQGIVLGGAAYMYNATQNDTSVWKSRLDQIVGGSTEFFFKDKVMYESTCQDSKTCNQDQRSFKSIFSRMLSLTRSLAPYTAETIDPLMEASAEGAAKSCTGGTDGHTCGLNWQLGYHDGYYGLGEQACALEGIQSLMYSKRPAPYTSDNGGSSVGDTEAALNSTSTNVLQSDIKITHKDKVGASIITAVILAVLVGGSIWMIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHS1</accession>
    <name>C5DHS1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0E06666p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0E06666g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553769.1">
        <property type="nucleotide sequence ID" value="XM_002553723.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHS1"/>
    <dbReference type="STRING" id="559295.C5DHS1"/>
    <dbReference type="GeneID" id="8291924"/>
    <dbReference type="KEGG" id="lth:KLTH0E06666g"/>
    <dbReference type="eggNOG" id="ENOG502QZSN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_311702_0_0_1"/>
    <dbReference type="InParanoid" id="C5DHS1"/>
    <dbReference type="OMA" id="SCAQIAP"/>
    <dbReference type="OrthoDB" id="762982at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050613">
        <property type="entry name" value="Sec_Metabolite_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001:SF90">
        <property type="entry name" value="CENTROMERE DNA-BINDING PROTEIN COMPLEX CBF3 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001">
        <property type="entry name" value="UNCHARACTERIZED TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00755">
        <property type="entry name" value="AFLATOXINBRP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="6">
        <location>
            <begin position="34"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="73"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="120"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="708"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="798"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="77"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="128"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="144"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="165"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR23332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="946" mass="105456" checksum="70D88FCCE3BC2894" modified="2009-07-28" version="1">MSSKLIPIKDQAALVLEDGKIYKIQHRRQRKILSCVPCHKRKIKCTREQPSCSKCLKRNWDCSYFLNDRVSRGGQPTAETRTEGSAQLPTNIVSGKHDPQKKKQLLMAIEKAKSMINDERNLLELNRKQKREYPRRKQAWETDQPVSGTVTPSGGHNQHEYPVASVTSGTSQSTPTISPPMSYSSMQDSPRPLSGENSLPMYLPTRQRAVELFEVYRNTVHPIIPLLDFSKFMSDQETFWNEVDQGESGNTDFLLILFPVLYAASKSQFHQCNDNDALFQEMASFLEATNVLYGIHDFPNNFTMQMITGSVLINSIIENPSVTTIAQLSRLAQRAMLSRDPASYHQITDLGLIQCRRILFWQIFQLDTMTSLYDNLPPLIKVDDFDTALPAEVVRGELDPSLCLLNAKYRFVLLLNELCSLTNRGTFQGLKERIVDLHVCCMGSALSLRNYNKENGRLTTSDAKFIDWAVFMLNTFADRACLLLHLNIIKTSLPILMKRRRIWKKELDSKPGAPSDPISESGYGQNFLTIQAILNDSGNLTLDHALMGLGDSNSAGLVYDYEDLTNNLIPASLHYLDEFLKYHSDDTYSCYNWELLVGNMPINAITFSLKTLALDLNRAQQMGEVLILQNDLRYILLSKAIPVAEMKVDPKTAVCKNCFQLIMLLFRLIIVKYGKAMQLLSDNQLDASKMFYSKYDVGNALLTSDRSGVVPQPSVGPMSGSHGSERHSQALNGSPADVDSSRDANRILSITSIINSEDLFNDGFSFSGNLIYNNNALDTSYTTSCAQIAPRIMRTLSSAPQNQSGAPNFGEAGPQPSQPAEGEANVSTANVYFNPYVHNMPLSPTPALAPLPAMATDYATANALYGNGKPAFQSAGAVSNESGSVGRTEPEVQPQPEIEWIRQEVQRHILLLNSDTGEMETIGTGDEYYREFENALLEIICGILSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHU1</accession>
    <name>C5DHU1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0E07106p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0E07106g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553789.1">
        <property type="nucleotide sequence ID" value="XM_002553743.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHU1"/>
    <dbReference type="GeneID" id="8291944"/>
    <dbReference type="KEGG" id="lth:KLTH0E07106g"/>
    <dbReference type="eggNOG" id="ENOG502T1IH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_754532_0_0_1"/>
    <dbReference type="InParanoid" id="C5DHU1"/>
    <dbReference type="OrthoDB" id="4034014at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR23352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="360" mass="40204" checksum="94F8F045A9C5487D" modified="2009-07-28" version="1">MPDMKKKSDKNDKIWNVVSFDRESRVQPQPQVSVELVTEEGLCDDILSNASSDESELPASPLKQSNTELSFGTLVGRNEDEDDFAAPERQEECSAPKTGSSLLRAITTLKTWHVVVLTSSTTLFLTYVVQSFLYNQATELGNETATVPYFTDHGASYRNVDFVLPFGDISENTSKFIVDFENRVAIPILAEVSPWQATKFQVNQKAACYWKGFKSFCNEHAETLSERFSGAGNALKNTLRRSGSLFAGRLGELAANFEPVKASASALGERLVQNYKDCDAYYRNNFPMCGRYIRGKAHGLVGNTRSLPRRLGALKPTLGANLRKALSTSDRIVQKADLYVLSRWNNTIEMLRFKKKNIIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DI82</accession>
    <name>C5DI82_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0E10450p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0E10450g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553930.1">
        <property type="nucleotide sequence ID" value="XM_002553884.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DI82"/>
    <dbReference type="FunCoup" id="C5DI82">
        <property type="interactions" value="164"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DI82"/>
    <dbReference type="GeneID" id="8292095"/>
    <dbReference type="KEGG" id="lth:KLTH0E10450g"/>
    <dbReference type="eggNOG" id="ENOG502QRUI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078018_1_0_1"/>
    <dbReference type="InParanoid" id="C5DI82"/>
    <dbReference type="OMA" id="FAHHNNL"/>
    <dbReference type="OrthoDB" id="360689at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05797">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001790">
        <property type="entry name" value="Ribosomal_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043141">
        <property type="entry name" value="Ribosomal_uL10-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047865">
        <property type="entry name" value="Ribosomal_uL10_bac_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11560">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L10, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00466">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160369">
        <property type="entry name" value="Ribosomal protein L10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR23493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="229" mass="25457" checksum="1CACE20529503FC1" modified="2009-07-28" version="1">MLTQSLGLFPRIPSSSLLSRGLRFASTEARITVKPLNSRKTLLMDTYKELMEKNPVVLFAHHNNLVKQEDAFFRSQIKELGGQMTVLRNQLFQVYLRNAHLPDPCAPTPKAEQNRTHPLLPLFKGPTAAITFPETEPAKVAKLLKLLEKSSEKLFVVGAKVDGGILDVPQLHQFKTLSSKPELQSQLLGLLHLLSGAGLVKTLEAGSHSLYLTLQSHHNNIDPENKKSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DI84</accession>
    <name>C5DI84_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Cytochrome c oxidase assembly protein COX20, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0E10494g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in the assembly of the cytochrome c oxidase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the COX20 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553932.1">
        <property type="nucleotide sequence ID" value="XM_002553886.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DI84"/>
    <dbReference type="FunCoup" id="C5DI84">
        <property type="interactions" value="274"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DI84"/>
    <dbReference type="GeneID" id="8292097"/>
    <dbReference type="KEGG" id="lth:KLTH0E10494g"/>
    <dbReference type="eggNOG" id="ENOG502S3BD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125578_0_0_1"/>
    <dbReference type="InParanoid" id="C5DI84"/>
    <dbReference type="OMA" id="IVGWEQC"/>
    <dbReference type="OrthoDB" id="14603at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022533">
        <property type="entry name" value="Cox20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31586:SF1">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX20, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31586">
        <property type="entry name" value="CYTOCHROME C OXIDASE PROTEIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12597">
        <property type="entry name" value="Cox20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007871">
        <property type="entry name" value="Cox20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR007871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR23495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="184" mass="21157" checksum="B1DCCB32F9355A4D" modified="2009-07-28" version="1">MVWPFSRRQNGSQDREPEAGPEINSKASGKKYLLEDTKPRFANESQSQYANAQEQATLRKAWETVDWQDFSFERLVRIPCFRDAGMTGFTSMFVLGSVMFLYHKNPSKAANWSVGGLMLGSIVGWEQCRLRRKNSFHTAQMAKSTVAAKEKPMLHTPVNDDRVKKEWEGHTSASTAKKPWYKFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIA4</accession>
    <name>C5DIA4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0E10956p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0E10956g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 34 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553952.1">
        <property type="nucleotide sequence ID" value="XM_002553906.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIA4"/>
    <dbReference type="FunCoup" id="C5DIA4">
        <property type="interactions" value="162"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIA4"/>
    <dbReference type="GeneID" id="8292118"/>
    <dbReference type="KEGG" id="lth:KLTH0E10956g"/>
    <dbReference type="eggNOG" id="KOG4748">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021434_0_0_1"/>
    <dbReference type="InParanoid" id="C5DIA4"/>
    <dbReference type="OMA" id="GWQKVDI"/>
    <dbReference type="OrthoDB" id="407658at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000117">
        <property type="entry name" value="Glycosyltransferase family 34 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008630">
        <property type="entry name" value="Glyco_trans_34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31306:SF5">
        <property type="entry name" value="ALPHA-1,6-MANNOSYLTRANSFERASE MNN10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31306">
        <property type="entry name" value="ALPHA-1,6-MANNOSYLTRANSFERASE MNN11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05637">
        <property type="entry name" value="Glyco_transf_34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR23515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="337" mass="40257" checksum="B3A308CA85B76C2C" modified="2009-07-28" version="1">MSSSMPYDPSFEDEKKRQYSYKHRSFLDRVGATIAQHKRAVLGIFCAFFVLFWFGSSRFTLWSKDPKIVLILAVNDGGGVHKWKGEQEWAIERISIQNKRAYAKRHGYGLTLKDMRTARRYSHEYREGWQKVEVLRQTMREFPKAEWFWWLDLETLIMEPQISLEEHIFKRLDSLSYRMLDKFNPLDLPLDIPYVDYSQPPELLITQDCGGFNLGSFLVKNSKWAHLLLDVWWDPVCYEQKHMMWEHREQDALEALYANQPWIREKTAFLPLRSVNAFPPGACSEFSDDPRYFYSEEDRDFVVNMAGCAFGRDCWGEMQRYGSLLESHNQKWWRRFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIJ4</accession>
    <name>C5DIJ4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BRO domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0E13002g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554042.1">
        <property type="nucleotide sequence ID" value="XM_002553996.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIJ4"/>
    <dbReference type="FunCoup" id="C5DIJ4">
        <property type="interactions" value="103"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIJ4"/>
    <dbReference type="GeneID" id="8292211"/>
    <dbReference type="KEGG" id="lth:KLTH0E13002g"/>
    <dbReference type="eggNOG" id="KOG2220">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_321635_0_0_1"/>
    <dbReference type="InParanoid" id="C5DIJ4"/>
    <dbReference type="OMA" id="YLKRSYG"/>
    <dbReference type="OrthoDB" id="2141925at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09242">
        <property type="entry name" value="BRO1_ScBro1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09237">
        <property type="entry name" value="V_ScBro1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.560">
        <property type="entry name" value="alix/aip1 in complex with the ypdl late domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.50">
        <property type="entry name" value="alix/aip1 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.280">
        <property type="entry name" value="alix/aip1 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025304">
        <property type="entry name" value="ALIX_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004328">
        <property type="entry name" value="BRO1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038499">
        <property type="entry name" value="BRO1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23030">
        <property type="entry name" value="PCD6 INTERACTING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23030:SF30">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE NON-RECEPTOR TYPE 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13949">
        <property type="entry name" value="ALIX_LYPXL_bnd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03097">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01041">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51180">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRO1" evidence="7">
        <location>
            <begin position="4"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="741"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="791"/>
            <end position="811"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00041284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR23605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="823" mass="92204" checksum="3C848D5880118482" modified="2009-07-28" version="1">MKTLLLPLKVKDTESINWSKALAAYLKRSYGSSTWSQFFNTKLAEDLDHLRNNANGTLAPEALLEQNFLYYAFLEQLHLRLGNNSTQLKLEFTWYDASYTSQSASQKYTQRTVAFEKSSVLYNLGALMTQVAKDKLESDIKVSITYMSKAFGIFQYLSENFLNSPSVDLQAENTSLLADICHAEAQELFLLKVINGPDVTKHASLIAKLSLMASSLYQKVLDTLNGNPDTNTSEVSYGEPKWKSIISCKCSVYKSISAYNNALALEQAGRFGDAIGFLQSANDFIISAMAQKLYVKDSIDLESIKSLIADKQKTLVKDNDFIYHDSVPPSTSLESIKSMDAVKMQTLPQQLDTYMEKVTEPADALFKGIIPLEVYEKESIYSEQKAALLRKELEVADTADWEYSSFIEFTTLPKLLRDLRDTYTLTGKKSSSGNPQLSIMRDQVNSWSRSVRSSPYNDVEAQMKLIIEKRKRVLELLALSPTESKENAVKLKSSLVAASKSDEKLFSFVEPYRHEINLLRNSNTLIKKWDQLSSFQSQEPSLLDLDDSKNEEILSKIGYISEQHEKLKVLKDERSRIVQDLKRDVNEDDITNVIIMKRGASDANLKQLFEKELEKFKPLSTRIEATIFKQSSIINAIKISLDELFKLTGVNEDASENDPNSLERNELYKKLNQAFTSFSVFANDLPKGLNFYDALLKMTSDLASSPSAKRSIDELKTAPSLRQMESDFGQLSLSETSNEGQFHTHAGAGLPPVPPRTYGGNSGAGLNSENPVPPIPPKRQVYGLAGLMSGGAQSDTQALEQNPTSFYNNPSVFDESLYSRYSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIX4</accession>
    <name>C5DIX4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">rRNA 2'-O-methyltransferase fibrillarin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Histone-glutamine methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0E15906g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-glutaminyl-[histone H2A] + S-adenosyl-L-methionine = N(5)-methyl-L-glutaminyl-[histone H2A] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50904"/>
            <dbReference type="Rhea" id="RHEA-COMP:12837"/>
            <dbReference type="Rhea" id="RHEA-COMP:12839"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30011"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61891"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyltransferase superfamily. Fibrillarin family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554172.1">
        <property type="nucleotide sequence ID" value="XM_002554126.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIX4"/>
    <dbReference type="FunCoup" id="C5DIX4">
        <property type="interactions" value="1192"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIX4"/>
    <dbReference type="GeneID" id="8292343"/>
    <dbReference type="KEGG" id="lth:KLTH0E15906g"/>
    <dbReference type="eggNOG" id="KOG1596">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059055_1_0_1"/>
    <dbReference type="InParanoid" id="C5DIX4"/>
    <dbReference type="OMA" id="WNPNKSK"/>
    <dbReference type="OrthoDB" id="1859733at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031428">
        <property type="term" value="C:box C/D methylation guide snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990259">
        <property type="term" value="F:histone H2AQ104 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008649">
        <property type="term" value="F:rRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000494">
        <property type="term" value="P:box C/D sno(s)RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000452">
        <property type="term" value="P:snoRNA guided rRNA 2'-O-methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000056">
        <property type="entry name" value="Fibrillarin like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000001">
        <property type="entry name" value="Fibrillarin like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00351">
        <property type="entry name" value="RNA_methyltransf_FlpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000692">
        <property type="entry name" value="Fibrillarin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020813">
        <property type="entry name" value="Fibrillarin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003276">
        <property type="entry name" value="PRK04266.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10335:SF17">
        <property type="entry name" value="FIBRILLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10335">
        <property type="entry name" value="RRNA 2-O-METHYLTRANSFERASE FIBRILLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01269">
        <property type="entry name" value="Fibrillarin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00052">
        <property type="entry name" value="FIBRILLARIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01206">
        <property type="entry name" value="Fibrillarin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00566">
        <property type="entry name" value="FIBRILLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="8" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="35"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR23735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="344" mass="36455" checksum="FF8C1593C9804D22" modified="2009-07-28" version="1">MAIRPLNSRHARPSPGTSRRRSRRIIQFTNFSIGSRGGRGGPRGGGRGGFGGGRGGARGGFGGGRGGARGGFGGGRGGPRGGARGGPRGGARGGARGGARGGAKVVIEPHKHAGVFIARGKEDLLVTKNIAPGDSVYGEKRVSVEEPSKEDGAPPTKVEYRVWNPFRSKLAAGIMGGLDELFIAPGKKVLYLGAASGTSVSHVADVVGPEGLVYAVEFSHRPGRELISMAKKRPNVIPIIEDARHPQKYRMLIGMVDAVFADVAQPDQARIIALNSHMFLKDQGGVVISIKANCIDSTVDAETVFAREVQKLRDERIKPLEQLTLEPYERDHCIVIGRYMRSGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJ20</accession>
    <name>C5DJ20_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="19">KLTH0F12804p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="ordered locus">KLTH0F12804g</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Mitochondrial inner membrane carrier protein that mediates the import of NAD(+) into mitochondria. Can transport NAD(+) by unidirectional transport or by exchange with intramitochondrially generated dAMP and dGMP. Also able to transport NAD(+) by exchange with AMP, GMP or deamido-NAD (+) in vitro.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>AMP(in) + NAD(+)(out) = AMP(out) + NAD(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:65424"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:65425"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>GMP(in) + NAD(+)(out) = GMP(out) + NAD(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:65420"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:65421"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>dAMP(in) + NAD(+)(out) = dAMP(out) + NAD(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:65412"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58245"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:65413"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>dGMP(in) + NAD(+)(out) = dGMP(out) + NAD(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:65416"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57673"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:65417"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>deamido-NAD(+)(in) + NAD(+)(out) = deamido-NAD(+)(out) + NAD(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:65428"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:65429"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554746.1">
        <property type="nucleotide sequence ID" value="XM_002554700.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJ20"/>
    <dbReference type="FunCoup" id="C5DJ20">
        <property type="interactions" value="339"/>
    </dbReference>
    <dbReference type="GeneID" id="8292969"/>
    <dbReference type="KEGG" id="lth:KLTH0F12804g"/>
    <dbReference type="eggNOG" id="KOG0764">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015166_6_1_1"/>
    <dbReference type="InParanoid" id="C5DJ20"/>
    <dbReference type="OMA" id="AFYNGMG"/>
    <dbReference type="OrthoDB" id="10266426at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051724">
        <property type="term" value="F:NAD transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990549">
        <property type="term" value="P:mitochondrial NAD transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000081">
        <property type="entry name" value="NAD+ transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000106">
        <property type="entry name" value="NAD+ transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044712">
        <property type="entry name" value="SLC25A32-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45683">
        <property type="entry name" value="MITOCHONDRIAL NICOTINAMIDE ADENINE DINUCLEOTIDE TRANSPORTER 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 16 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 16 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 17" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="138"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="177"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="16">
        <location>
            <begin position="73"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="16">
        <location>
            <begin position="171"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="16">
        <location>
            <begin position="275"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00050736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00051263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00052051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00052827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00052961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00059403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAR24309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="380" mass="42006" checksum="158B2D117995B7F0" modified="2009-07-28" version="1">MSLQNQDGIVTTAAQLLVSGARKRPHLIYDEILAPDCSTPLSSADVLEDFTRLRTGGNDNNGSPLPTADKALDDTQITALSGAVAGFLAGITVCPLDVAKTRLQAQGLSSRLPNYYNGILGTLNTIVRDEGVRGLYKGLVPIIMGYFPTWMIYFSVYESSKKIYPQVFPSFDFLSHSASALTAGAISTILTNPVWVVKTRLMLQTHVNENSTRYKGTFDAFHKIYTTEGIKTFYSGLLPSLFGLFHVAIHFPIYEKLKIWLHCYPSIAASDDYNLNLARLIAASSASKMVASALTYPHEILRTRMQIRAPPESLAATEMKASSHSLIRLVRQTYRTEGLRGFYSGFTANLVRTVPASAITLVSFEYFRKYLNKLNDSFTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJC1</accession>
    <name>C5DJC1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Vacuolar protein sorting-associated protein 17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0F15202g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the membrane-associated retromer complex which is essential in endosome-to-Golgi retrograde transport.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the retromer complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the VPS17 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554847.1">
        <property type="nucleotide sequence ID" value="XM_002554801.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJC1"/>
    <dbReference type="FunCoup" id="C5DJC1">
        <property type="interactions" value="95"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJC1"/>
    <dbReference type="GeneID" id="8293076"/>
    <dbReference type="KEGG" id="lth:KLTH0F15202g"/>
    <dbReference type="eggNOG" id="KOG2273">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028982_1_0_1"/>
    <dbReference type="InParanoid" id="C5DJC1"/>
    <dbReference type="OMA" id="FYLGTME"/>
    <dbReference type="OrthoDB" id="9976382at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030905">
        <property type="term" value="C:retromer, tubulation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06891">
        <property type="entry name" value="PX_Vps17p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014461">
        <property type="entry name" value="Retromer_complex_Vps17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053055">
        <property type="entry name" value="VPS17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037907">
        <property type="entry name" value="Vps17_PX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47433">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47433:SF1">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF011791">
        <property type="entry name" value="Vps17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PX" evidence="3">
        <location>
            <begin position="103"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="482"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="520"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="489"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="524"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR011791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR24410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="554" mass="61956" checksum="802BB24D7ECB964D" modified="2009-07-28" version="1">MSSAVAYDPYDDIDNNPFSEPEANPQRSVSPDLRAPDDTHPSIGAQEATQGNTDSHAGAQPEPEQGMTTAQHGNPPGDFPEASTTAAAAEGADALLPERRSSKFQLVVKVTGLERVGSFTNKKENPTVKFDVSTNLPTFRKQQHRSLKKTFTEFRNLRKFLYGLISGTFVPALPSPSTNYGISNSEDYCKTVSNMQEWFDRIAVDPFILRSEELAFFIESDFDTYTPVGKTKLPLSGLKRKTLKQLAPPFDECLELAEFRPLVKSVHHLSQDIQTKLLKLCKLRKSLSHDENSIGQGFKELCMDQSQSHSKFYNKFGKTLSAVGDIDSVMATLDIATLYDGLEWLVQETYSVKEALTNRHFLMRDLLQAQQTSSSRQEQARKLRARRDVSPLKVDEAIRALKAATTAEHTLTLKLRRVTANMLLEKQRWLEWCDHQLQTSIKEYTLRKIEYERKKLSLLERIRADVRAADEAGGLSRLGRGARLSASGVSPSQTAQGDSWTSDPRSRAFEPSVLRTEFDASLESDTRDVHDTHPEDQHALDARSAASLLGTSSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJH2</accession>
    <name>C5DJH2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">Protein BCP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0F16412g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in nuclear export, actin cytoskeleton organization and vesicular transport.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the BCP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554898.1">
        <property type="nucleotide sequence ID" value="XM_002554852.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJH2"/>
    <dbReference type="FunCoup" id="C5DJH2">
        <property type="interactions" value="1064"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJH2"/>
    <dbReference type="GeneID" id="8293131"/>
    <dbReference type="KEGG" id="lth:KLTH0F16412g"/>
    <dbReference type="eggNOG" id="KOG3034">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068770_2_1_1"/>
    <dbReference type="InParanoid" id="C5DJH2"/>
    <dbReference type="OMA" id="VKFYRKE"/>
    <dbReference type="OrthoDB" id="27543at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025602">
        <property type="entry name" value="BCP1_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13261">
        <property type="entry name" value="BRCA2 AND CDKN1A INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13261:SF0">
        <property type="entry name" value="BRCA2 AND CDKN1A-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13862">
        <property type="entry name" value="BCCIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028983">
        <property type="entry name" value="BCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR028983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR24461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="266" mass="30489" checksum="D056ABA2955BEF45" modified="2009-07-28" version="1">MVAIKLSDAIKRKREPQDAESDIDISSTDSENESQVDDEQEVINIDFDFFNANKNVDFHALKNLLRQLLGPQESSKIQLSALADLILDSPTTTIKTDGQESDPYCFLSFIDYRSNRTSDYAKYLSKVDSRLAEFLKSMDNSDKKCALVLSERLINMPPEVIAPIYNITLEDASNSLGDGKHYDYYVIVSRKFEVNVEVDDEETGARKRVKTSEIDYFHEEDRFLERNAYVHFDSESRKGLISSYVVIDHDSLIKSIKDVEAEIKNW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJU4</accession>
    <name>C5DJU4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0F19184p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0F19184g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATP11 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555020.1">
        <property type="nucleotide sequence ID" value="XM_002554974.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJU4"/>
    <dbReference type="FunCoup" id="C5DJU4">
        <property type="interactions" value="340"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJU4"/>
    <dbReference type="GeneID" id="8293256"/>
    <dbReference type="KEGG" id="lth:KLTH0F19184g"/>
    <dbReference type="eggNOG" id="KOG3281">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054226_2_0_1"/>
    <dbReference type="InParanoid" id="C5DJU4"/>
    <dbReference type="OMA" id="FLQWGFH"/>
    <dbReference type="OrthoDB" id="16535at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033615">
        <property type="term" value="P:mitochondrial proton-transporting ATP synthase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010591">
        <property type="entry name" value="ATP11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13126:SF0">
        <property type="entry name" value="ATP SYNTHASE MITOCHONDRIAL F1 COMPLEX ASSEMBLY FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13126">
        <property type="entry name" value="CHAPERONE ATP11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06644">
        <property type="entry name" value="ATP11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="62"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR24583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="323" mass="37184" checksum="300E79F1A797E631" modified="2009-07-28" version="1">MRSVARALPRFATTQTRRASAISLTNFIQLQQSSNLKSNYSTESLDVRYKNKLLQKAEEQGFKSVEELKEKLKEDIETKKKELSKVDPLKELEEYEQRLKMSQNNMKVTQQRGPVDPKQEPAPFKTLDSFLKLEKIKDLSKQEVEFLWRARWANKPDALSAVVPLEIFEKMDRHIRACPTFVLPLPREVPAEASSSGTQEQGMELHYIQWQMADSNTVHCIMTSLAEYKLHSEFAKPHTTFQFHKELASDKKIILMNGHVEKDVNITLPDAQLLLLNIQRFYGAMGEETVSSKQRTKLLHDFAKGSPEFSVEKLINLAQSMET</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJV0</accession>
    <name>C5DJV0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0F19316p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0F19316g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555026.1">
        <property type="nucleotide sequence ID" value="XM_002554980.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJV0"/>
    <dbReference type="FunCoup" id="C5DJV0">
        <property type="interactions" value="30"/>
    </dbReference>
    <dbReference type="GeneID" id="8293262"/>
    <dbReference type="KEGG" id="lth:KLTH0F19316g"/>
    <dbReference type="eggNOG" id="ENOG502QQA7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021993_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJV0"/>
    <dbReference type="OMA" id="ALQLLYW"/>
    <dbReference type="OrthoDB" id="413079at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000286">
        <property type="entry name" value="MFS efflux transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051788">
        <property type="entry name" value="MFS_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23514">
        <property type="entry name" value="BYPASS OF STOP CODON PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23514:SF3">
        <property type="entry name" value="BYPASS OF STOP CODON PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="121"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="145"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="181"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="208"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="264"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="299"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="333"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="359"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="394"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="423"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="56"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR24589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="458" mass="51021" checksum="F45447EBBE10E375" modified="2009-07-28" version="1">MSTNSKSSDSIELENLLENDGDFSIPTHELREVEYNGELIKLYPLDYQKEPIVRWQIATSLLLFAVFGMNDECNGVLLPALTEHYNVTKVQVANLFIVQFCGYGTASLLTERIHRKVGARGAIIAAASMCVLCYGTLMLRPPWFAMYVMCFLPLGLAIGILDSVCNVLFGNLEIHKNEWMGVLHGIYGAAAMITPPVVTYFVKAGKWSFFFLLPLLCALGGLALVPFAFRDETANKYNYVCSTNREEGDDSHPGFWDLLRRPAILMYALYMFIYLGSEVSTGAWIFTYLLEVKKGNVVAMSYVTAAFWLGLTTGRLVLGFVTKRCFKNEYRASSFYGNMCFLFYTAFMVVAYFNGSSTLYFILLAFIIFLGGVFIGPLFPNASVVALQVLPKNLHISGVGIAVAVGGCGSAILPYAVGVITKYIGFVWFPFLCWFMVGTVSIVWFCYPKLIKGHSEYL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-05-31" modified="2025-06-18" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJV1</accession>
    <name>RTC1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName>Restriction of telomere capping protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">RTC1</name>
        <name type="ordered locus">KLTH0F19338g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in a process influencing telomere capping.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the WD repeat RTC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555027.1">
        <property type="nucleotide sequence ID" value="XM_002554981.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJV1"/>
    <dbReference type="SMR" id="C5DJV1"/>
    <dbReference type="FunCoup" id="C5DJV1">
        <property type="interactions" value="144"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJV1"/>
    <dbReference type="GeneID" id="8293263"/>
    <dbReference type="KEGG" id="lth:KLTH0F19338g"/>
    <dbReference type="eggNOG" id="KOG0269">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008512_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJV1"/>
    <dbReference type="OMA" id="GRDGKCC"/>
    <dbReference type="OrthoDB" id="60955at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061700">
        <property type="term" value="C:GATOR2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016239">
        <property type="term" value="P:positive regulation of macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16488">
        <property type="entry name" value="mRING-H2-C3H3C2_Mio-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037590">
        <property type="entry name" value="WDR24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049566">
        <property type="entry name" value="WDR59_RTC1-like_RING_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46200">
        <property type="entry name" value="GATOR COMPLEX PROTEIN WDR24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46200:SF1">
        <property type="entry name" value="GATOR COMPLEX PROTEIN WDR24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17120">
        <property type="entry name" value="zf-RING_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0926">Vacuole</keyword>
    <keyword id="KW-0853">WD repeat</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000408785" description="Restriction of telomere capping protein 1">
        <location>
            <begin position="1"/>
            <end position="1290"/>
        </location>
    </feature>
    <feature type="repeat" description="WD 1">
        <location>
            <begin position="162"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="repeat" description="WD 2">
        <location>
            <begin position="211"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="repeat" description="WD 3">
        <location>
            <begin position="259"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="repeat" description="WD 4">
        <location>
            <begin position="318"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="repeat" description="WD 5">
        <location>
            <begin position="397"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="repeat" description="WD 6">
        <location>
            <begin position="447"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="repeat" description="WD 7">
        <location>
            <begin position="778"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="repeat" description="WD 8">
        <location>
            <begin position="821"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="repeat" description="WD 9">
        <location>
            <begin position="1079"/>
            <end position="1119"/>
        </location>
    </feature>
    <feature type="repeat" description="WD 10">
        <location>
            <begin position="1166"/>
            <end position="1205"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="RING-type; degenerate" evidence="2">
        <location>
            <begin position="1243"/>
            <end position="1285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="732"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="856"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="960"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1004"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="36"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="750"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="858"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="896"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="972"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1018"/>
            <end position="1034"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="1290" mass="142913" checksum="86AA2BF319A19012" modified="2009-07-28" version="1">MSRSQSPSLNDGLSTSNSRHSSSHSRFSLHKAFPQLSGNASFNGTSPKTKLPSRFSGGSNPPYMDSLKEQESSSSSSRSNFRRANYVNPGRIRSSGLKYSLQAPKELSSIDKINDPASRSIVISGKNHLGLYRFREDYGKLELTLDLFNIDQKAASLRTTTRKTSTISDVKAGFHNYKNYVAICGTSTSVSIYDINRANLVDGPASMVLSKHTRSINSVDFNMAQTSLLISGSQDGCIKVWDLRSSQGRKNKSDLTINSGSDSVRDVKWMPTYDFPNNDDVVAGPSNRSHRFASIHDSGLLLTYDLRQPSQAEKRINAHSGPGLCLSWHPTLDYIMTGGRDGKCCLWNMGSKPLNPASFQNIHHNSSFSTSAASSFTQGFSANSVTNFINSPEAVVNTAHPLTKLKFRPSAIPEVFDSVIALSSLGENSDVSLYSLSRSYIPKNVLTTNSPSCGFVWWNENLIFNIDKQNMVTGWDLGKEPTVLDNLPKNKIAWRDIEGDGLVFLAQDKGGYESGGPDGMSVSTETRNFSQSRLSTTTMSNLFPSHTMRQNSSVSSFPALNTHSNSGANLGDRPSLPRTVTSYSKGAYTQTYGSYNTHSSHHNSAASASASSIATELGSEHVLSPRMISLDLPHILNSIRSAKIENYAKKPSRPGSAAFKDSPVEVFKFLSRELKFSYMHDRNFNKPENLTDQDDLAQSVDDNDLKSHLITRFGFSENNTWTKFIKKSNSPEKDAAVENGTKVNEASIEKVTNSPNDTGSDELSSISKLSTEPKKSDLAERNITAIRQRVKHFIELVSMCDHNAEIYLYIDDLSNFKIWMMMRDSLLWELKQITDSLETDGGSLLSESLLVNPDVTRHSNGARKQSMASDYSSFSTSEVGSTTGVQALPEFRGPHSLSTPPRATTSFGAKEPSSLKKRESTIDENLSETEIEAKGLPKEDIKNLRNQISKHQIGDEVAIEDEEEDKFEEKSSAGTDASNKNIPIIPSERKVSFVDQFINSMRSPKTLHGDFETDGSRMANSASTKRSSQPSFSSSLAGLITRRLSDQSPKTKPKDLIYSKSSGSIAKNSSDLFDFQNVEGNNSPRVLTQKSKISMLLKGLKKSETAPPWDASRLIKKIFEQSVATGNVLLTISIILLFQTTFKVTSTTVVKNALSEFINILHKYELFEIAANLLKFCPWDDILEAGNGQCTVRIYCERCKKLLVNESSKEKFTEEWKRTGRQEVMKRFGYWYCDNCSKRNTLCVLCEQPLKKLTFCVLNCGHEGHPECFKKWFMNEEMSECPSGCSGFLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJY1</accession>
    <name>C5DJY1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0F00176p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0F00176g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VEL1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554219.1">
        <property type="nucleotide sequence ID" value="XM_002554173.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJY1"/>
    <dbReference type="FunCoup" id="C5DJY1">
        <property type="interactions" value="31"/>
    </dbReference>
    <dbReference type="GeneID" id="8292406"/>
    <dbReference type="KEGG" id="lth:KLTH0F00176g"/>
    <dbReference type="eggNOG" id="ENOG502RZUS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1349595_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJY1"/>
    <dbReference type="OMA" id="GLHGPHC"/>
    <dbReference type="OrthoDB" id="4039163at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019435">
        <property type="entry name" value="Vel1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10339">
        <property type="entry name" value="Vel1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002947875" evidence="6">
        <location>
            <begin position="21"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR23782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="210" mass="23197" checksum="5E7B5D4A345BA6B6" modified="2009-07-28" version="1">MWLSTLFLFVTGILSTSVAAFRYDWSNITCKGLHGPHCGTYLLKVKNQNDTYLGQSYFVGADALANDATDAWGRLLHEEYRFIPRLTTVQTLNDTGNFRPFVGTTDMSTCNFQSVENAVVPYINTVTNELSYDSWASISMNATAVTGVAPQLLKASTYGIQVAICNPGFITDLFQSPTVNLFNVEDTLPSWCEAIEVEAVCPADVNYDTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJZ5</accession>
    <name>C5DJZ5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0F00572p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0F00572g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554233.1">
        <property type="nucleotide sequence ID" value="XM_002554187.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJZ5"/>
    <dbReference type="GeneID" id="8292424"/>
    <dbReference type="KEGG" id="lth:KLTH0F00572g"/>
    <dbReference type="eggNOG" id="ENOG502S4ZE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118179_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJZ5"/>
    <dbReference type="OMA" id="GSKIWFV"/>
    <dbReference type="OrthoDB" id="4063222at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="195" mass="21658" checksum="F950611CD5A08C48" modified="2009-07-28" version="1">MDTHPGKSRPLSKRGSRSCPDKPSTLEGQGKNIESALVSGLETIAGIFDDLQLLRSFGVIGENNVFYQKLNKSGFCSKAWLVSLTLSSRRNASDIINLAISRSRLKREQAEFMRRPVNPVRKVLNAKVTARIQEINQKLILVALELIQNIGYLTLVAADVLAFGLTEKWKRLLERVSSIFAIARLLFSGFSSLTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DK17</accession>
    <name>C5DK17_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ER membrane protein complex subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0F01056g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Component of the ER membrane protein complex (EMC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EMC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554255.1">
        <property type="nucleotide sequence ID" value="XM_002554209.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DK17"/>
    <dbReference type="FunCoup" id="C5DK17">
        <property type="interactions" value="837"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DK17"/>
    <dbReference type="GeneID" id="8292446"/>
    <dbReference type="KEGG" id="lth:KLTH0F01056g"/>
    <dbReference type="eggNOG" id="KOG2103">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005034_3_0_1"/>
    <dbReference type="InParanoid" id="C5DK17"/>
    <dbReference type="OMA" id="PIPEQKL"/>
    <dbReference type="OrthoDB" id="28092at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026895">
        <property type="entry name" value="EMC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011678">
        <property type="entry name" value="EMC1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21573">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21573:SF0">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07774">
        <property type="entry name" value="EMC1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="27"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="740"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="domain" description="ER membrane protein complex subunit 1 C-terminal" evidence="12">
        <location>
            <begin position="552"/>
            <end position="767"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF07774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR23818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="771" mass="87366" checksum="2AADD5B3D2F6902B" modified="2009-07-28" version="1">MALSFNVWTIYLRVDRNSLASLKQPRLLVFTLCPIMLGAFRSFIGITLLVVIKGLFVEAIFSDEAYHIDWQLSNVGPYQCVLPASATESNDQLLVLSTFNNGTMISWLNKTSGELLERLPLGYRVADAMIEHNDLFLKLEDGSINAVDSMNGFSLDHPPTEFSSSCNPDLSTIRFEDGKIKIMDHDTDLQIFSIDMPQDFKGIKYFRSDKENTLDLMYSTVGGKYTFTSVSDLTSTQTWTRDESLANIRAYTYVSSADPNLAQMFTELSQEGSMNVLEAYQFRVKQTLNRLKNYLLEKKFSVGAIVKEFLEDEDEATLHQKDVSFGLLKYLIVATEEGKIAVLDVRDGRQLWNYESGLKDILNIEALRSDTEVVIFTKSGPSLTFDITRIETEPYLLTNTNMAPVHSVEKISENGAFLFKRPGGGRFLLENPLNPITQDLYLLDHNSKELNAFIYQNEAITETWKTKVDEQEEIVAFSSRGEEHVANLGIVLGNRTVLYKYLYPHLAAYATKNEENVLTIRIIDTVSGELLHTSFHEDEVDFNFPINLVFGEHWCVYTYFSTKPVPEQKIAVIELYESLVANERVSEPLTEVNALENIQRPQFIQKAYLYPEVIKNIGLTKTKLGITTKSIILELENGQVTILPKFILSARRVEESQLSADDKKEFMVMPYLSAIPINDQAVLTHNRVLLTGPDSHLLSIPTDLESTTLVCTLGHDLFCTRVFPSSQFDKLSPSFEKGKLMATVIGLLLICFFLRPLVDTKKLKLKWLVKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-04-02" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DK37</accession>
    <name>C5DK37_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0F01496p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0F01496g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554275.1">
        <property type="nucleotide sequence ID" value="XM_002554229.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DK37"/>
    <dbReference type="STRING" id="559295.C5DK37"/>
    <dbReference type="GeneID" id="8292466"/>
    <dbReference type="KEGG" id="lth:KLTH0F01496g"/>
    <dbReference type="eggNOG" id="ENOG502S2FC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_664055_0_0_1"/>
    <dbReference type="InParanoid" id="C5DK37"/>
    <dbReference type="OMA" id="GNDDNKY"/>
    <dbReference type="OrthoDB" id="3981262at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="189"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="211"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="254"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="285"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="332"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="367"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="409"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="429"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="455" mass="48570" checksum="6431E42D6359D5F2" modified="2009-07-28" version="1">MPNYACHVLHSLLSMWPKPASLTYFSSWHLFRERFEKLECTRLQLRFNTWSSDTDQAKQNYRMNVDQLINQAQQLFEQGRHQEAVESLTTATKQDPSPNQQSYIEETINKIGQHVVNNGDESGTRGIGGSLMSAFMGGQGNSGYGGSQSQLSKLALLSTILGSSNKNSGSFNLGSVASMFTGSGKQNTQNYGNNNYGNNNYGNPQNNYGYNNGGNQSGSGSGLSSLAGKLFGGKHSSQQNNTPPPPPPQQQQQSGSFGSLASMTSSFLGGGKQSNHGNQVPPPQQYGNPQQGSYGQMYNSGSQGQQQGQYGNYGEQGNYGPPGGHRPQGNHGPPGGFGSQGGNFGGRQGNSGSHENFGQGGGYGQTGRHEQQGRFENQDNFGSQGNYGQGGYGQQNNFSESGFEGRRGGQASEYYQGQQSGSGQGYQRPSDNQNQESSGQGFSFAGNFVPGNGRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKI3</accession>
    <name>C5DKI3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">GPI ethanolamine phosphate transferase 1</fullName>
            <ecNumber evidence="13">2.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">KLTH0F04884g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Ethanolamine phosphate transferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers ethanolamine phosphate to the first alpha-1,4-linked mannose of the glycosylphosphatidylinositol precursor of GPI-anchor.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Endoplasmic reticulum membrane</location>
            <topology evidence="1 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the PIGG/PIGN/PIGO family. PIGN subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.-.-.-" evidence="13"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002554375.1"/>
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    <dbReference type="STRING" id="559295.C5DKI3"/>
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    <dbReference type="eggNOG" id="KOG2124">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007676_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKI3"/>
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        <property type="component" value="Chromosome F"/>
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        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:mannose-ethanolamine phosphotransferase activity"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="P:cell wall organization"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:GPI anchor biosynthetic process"/>
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    <dbReference type="CDD" id="cd16020">
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        <property type="entry name" value="GPI ethanolamine phosphate transferase 1"/>
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        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
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    </dbReference>
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        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
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        <property type="entry name" value="GPI_EtnP_transferase_1"/>
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        <property type="entry name" value="GPI_EtnP_transferase_1_C"/>
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        <property type="entry name" value="PIGN_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000917">
        <property type="entry name" value="Sulfatase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12250:SF0">
        <property type="entry name" value="GPI ETHANOLAMINE PHOSPHATE TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12250">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL GLYCAN, CLASS N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PigN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="8 13" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 13" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="458"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="490"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="516"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="554"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="599"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="656"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="689"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="720"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="774"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="816"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="851"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="883"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfatase N-terminal" evidence="14">
        <location>
            <begin position="233"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="GPI ethanolamine phosphate transferase 1 C-terminal" evidence="15">
        <location>
            <begin position="442"/>
            <end position="875"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008400"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020831"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU367138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF04987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAR23984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="922" mass="104722" checksum="CFE03FB5E04BC51A" modified="2009-07-28" version="1">MWSKQRLTFVVVGILFHFVYLWSIFDIYFVSPLVHGMQTYRSTDAPPAKRLFLIVGDGLRADTTFDKITHPTTGETAHLAPFLRSLVLNNATYGVSHTRMPTESRPGHVAMIAGFYEDVSAVTKGWKENPVDFDSFFNQSAHTYSFGSPDILPMFKDGASDPNKVDAWMYGHEFEDFTQSSIELDAYVFRHLDNLFYNSTMDRELHEQIMQPGNVFFLHLLGCDTAGHSYRPYSPEYYDNVKYIDDEVSKLVPKVRDFFGDDDTAFVFTADHGMSAFGSHGDGHPNNTRTPLIAWGAGLNRPVLNKAPIYDNYTEGWDLANIQRNDVKQADIASLMTYLIGADYPANSVGELPLSYIDGSEENKLKALYSNAKAILEQYLVKEQEIELSQFQYKPYHKFVEKPASQYLDEIEQLIERISAGDESLESPAIKLTEELMSVTLEGLHYLTTYNWQFTRTIVTFGFVGWITYSFIIFLRLFILTKEYELKTTITNLAIFGALSLVLNYVLHYQRSPFNFYMYLMFPLFFWSQIFKSRVILGDGLGEFFEGITLPKKLGILAAVLAIYESIVYGFFSRWIFTVSFVVLGFYPLACGIKDVRTNILWLATSLSISVFTLFDAVKVESLKQINAASALIVLSACWGIYHLRQDIKAHTSKVLVIQVVLIVAMVAITNKSVISLQNREGLPRDSQIGGWIVFALSLAVMPIFHYLEPSNDYRVRMLIIYLTFAPAFIILTISFESFFYFLFTAYIVQWIEIESGIKENSAKNASSHNWLQLLRVSVIGFFLMQVAFFSTGNIASISSFSLDSVYRLMPIFDPFPMGALLVLKLILPYITLSSGLGILNLKLRFKDYSISSLIICTSDILSLQFFYLLKTEGSWLDIGVSISNYCLAILSSLIMLLLEFISHVLLRNVSLSRADKVKKTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKS7</accession>
    <name>C5DKS7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2-dehydropantoate 2-reductase</fullName>
            <ecNumber evidence="4">1.1.1.169</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Ketopantoate reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0F07172g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the NADPH-dependent reduction of ketopantoate into pantoic acid.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(R)-pantoate + NADP(+) = 2-dehydropantoate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16233"/>
            <dbReference type="ChEBI" id="CHEBI:11561"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15980"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.169"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ketopantoate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.169" evidence="4"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554515.1">
        <property type="nucleotide sequence ID" value="XM_002554469.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKS7"/>
    <dbReference type="FunCoup" id="C5DKS7">
        <property type="interactions" value="147"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKS7"/>
    <dbReference type="GeneID" id="8292718"/>
    <dbReference type="KEGG" id="lth:KLTH0F07172g"/>
    <dbReference type="eggNOG" id="ENOG502QPT5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031468_10_2_1"/>
    <dbReference type="InParanoid" id="C5DKS7"/>
    <dbReference type="OMA" id="GMIDEVN"/>
    <dbReference type="OrthoDB" id="73846at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008677">
        <property type="term" value="F:2-dehydropantoate 2-reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015940">
        <property type="term" value="P:pantothenate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.10:FF:000047">
        <property type="entry name" value="2-dehydropantoate 2-reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003710">
        <property type="entry name" value="ApbA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050838">
        <property type="entry name" value="Ketopantoate_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013752">
        <property type="entry name" value="KPA_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013332">
        <property type="entry name" value="KPR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00745">
        <property type="entry name" value="apbA_panE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43765:SF2">
        <property type="entry name" value="2-DEHYDROPANTOATE 2-REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43765">
        <property type="entry name" value="2-DEHYDROPANTOATE 2-REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02558">
        <property type="entry name" value="ApbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08546">
        <property type="entry name" value="ApbA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0566">Pantothenate biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950520" description="2-dehydropantoate 2-reductase" evidence="10">
        <location>
            <begin position="31"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="Ketopantoate reductase N-terminal" evidence="11">
        <location>
            <begin position="8"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="Ketopantoate reductase C-terminal" evidence="12">
        <location>
            <begin position="216"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF08546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR24078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="374" mass="41895" checksum="C43D95EFFE1D9A4E" modified="2009-07-28" version="1">MTQTRPKVFVLGFGSIGALLASCLQQQTQSVVVPLLRSRERLGDFKKLSFKAGIRALYQKDKPISESVFENASCPELFPQDWRIDNLIITTKTYQTKEALAPYMKYIHSDTNIILVQNGLGVFEVLNDEIFLDYKPNLFQGVISHGIFHDTGFTFNHAGFGDMKVARLPSEGPGSIVQSNTLIADNAKNNELIRLMCSPGYAEALAVKHMTYQEMLLGQLEKFLVNACMNPVTSIVDCINGEFKDCAAPIFSAIVTEALDILRVAYRPLFDYKPQSDDYPTLDVASILDADRMLDFIIHVGCVVNKENSSSMRQDVVNVRETEIDYINGYIVTLCEKLGLGPERCKVNQTIATLVKLRLELNRTRKLHGDKRAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKV6</accession>
    <name>C5DKV6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0F07854p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0F07854g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2022" name="Nat. Chem. Biol." volume="18" first="1253" last="1262">
            <title>Structural basis for activation of fungal sterol receptor Upc2 and azole resistance.</title>
            <authorList>
                <person name="Tan L."/>
                <person name="Chen L."/>
                <person name="Yang H."/>
                <person name="Jin B."/>
                <person name="Kim G."/>
                <person name="Im Y.J."/>
            </authorList>
            <dbReference type="PubMed" id="36229681"/>
            <dbReference type="DOI" id="10.1038/s41589-022-01117-0"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.59 ANGSTROMS) OF 471-788</scope>
        <scope>DISULFIDE BONDS</scope>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554544.1">
        <property type="nucleotide sequence ID" value="XM_002554498.1"/>
    </dbReference>
    <dbReference type="PDB" id="7VPU">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.59 A"/>
        <property type="chains" value="A/B/C/D=471-788"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKV6"/>
    <dbReference type="SMR" id="C5DKV6"/>
    <dbReference type="FunCoup" id="C5DKV6">
        <property type="interactions" value="306"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKV6"/>
    <dbReference type="GeneID" id="8292749"/>
    <dbReference type="KEGG" id="lth:KLTH0F07854g"/>
    <dbReference type="eggNOG" id="ENOG502QRM1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011669_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKV6"/>
    <dbReference type="OMA" id="EVWPTIT"/>
    <dbReference type="OrthoDB" id="416217at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053157">
        <property type="entry name" value="Sterol_Uptake_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47784">
        <property type="entry name" value="STEROL UPTAKE CONTROL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47784:SF5">
        <property type="entry name" value="STEROL UPTAKE CONTROL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="5" id="KW-0002">3D-structure</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="2">
        <location>
            <begin position="49"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="245"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="356"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="364"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="374"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="386"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="447"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="655"/>
            <end position="723"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR24107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="7VPU"/>
        </source>
    </evidence>
    <sequence length="788" mass="86213" checksum="08C5058B82140289" modified="2009-07-28" version="1">MSSEGSDEDAMYRSHRRKEKVIELIEVDGKKVSKTSTGKRKFHKKSKNGCDNCKRRRVKCDEDKPSCQKCINMKLECVYTPVASRKRKSSSSAHPPQQAVAPKHEGSPSLVGTPGPGGSGVLQSKASTFAPTSRKPSIAVGDIQKHSQHPTPTSGPLGSQDNVLLPPLFPGANMQLQQQIQQQPQLLPQLMTLSNSEKFAGFDFPNSPPFQSTSMSLTNSIGSIFAANKLPSNVLNNVINQQQQQQQQQQQQQQHSRQSQQVQPHLQQQHQPHLQQQHQQHQASPLSSLLHSHEMAQTLSKAGINLKQMAMFSTAGIGGVTYDIQEVLGMKPQGNGDPHNKDGATTAEEVLATLQKQQQQMRNSHHEKEEQKLSDGQTQNGDTSGANLDPNTAANLANNPTISPGAPMSPMAKSATAPMDTDDHTEFYTTKDTSGGTAGLSSKMMDKSNTQDSTSTIESQPGTIARLVQLSNQGTLNLIDLKLFHHYCTEVWPTITSAGISGERIWSDEIPQLAFDYPFLMHALLAFSATHLARKEPGLEQYVASHRLDALRLLRKAVLEISEDNTDALVASALILIMDSLANASSSSSAVSESLAASSMSPSAWIFHVKGAATILTAVWPLTEKSRFHNLISVDLSDLGDIINQDSGTVSELVCFDESIADLYPVEIDSPYLITLAYLDKLHREKNQSDFILRVFAFPALLDKTFLALLMTGDLGAMRIMRCYYQLLRGFATEVKDKVWFLEGITQVLPQDVDDYSGGGMHMMLDFLGGGLPSMTTTNLQPGLSDFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKW9</accession>
    <name>C5DKW9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0F08162p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0F08162g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the purine-cytosine permease (2.A.39) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554557.1">
        <property type="nucleotide sequence ID" value="XM_002554511.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKW9"/>
    <dbReference type="FunCoup" id="C5DKW9">
        <property type="interactions" value="95"/>
    </dbReference>
    <dbReference type="GeneID" id="8292763"/>
    <dbReference type="KEGG" id="lth:KLTH0F08162g"/>
    <dbReference type="eggNOG" id="KOG2466">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021555_3_0_1"/>
    <dbReference type="InParanoid" id="C5DKW9"/>
    <dbReference type="OMA" id="GLCVNMI"/>
    <dbReference type="OrthoDB" id="2018619at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903089">
        <property type="term" value="F:5-amino-1-ribofuranosylimidazole-4-carboxamide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015205">
        <property type="term" value="F:nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015888">
        <property type="term" value="P:thiamine transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11482">
        <property type="entry name" value="SLC-NCS1sbd_NRT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.4160.10:FF:000005">
        <property type="entry name" value="Thiamine transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4160.10">
        <property type="entry name" value="Hydantoin permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012681">
        <property type="entry name" value="NCS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001248">
        <property type="entry name" value="Pur-cyt_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045225">
        <property type="entry name" value="Uracil/uridine/allantoin_perm"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00800">
        <property type="entry name" value="ncs1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30618">
        <property type="entry name" value="NCS1 FAMILY PURINE/PYRIMIDINE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30618:SF15">
        <property type="entry name" value="NICOTINAMIDE RIBOSIDE TRANSPORTER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02133">
        <property type="entry name" value="Transp_cyt_pur"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="121"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="205"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="284"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="378"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="404"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="491"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="560"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="565"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="577"/>
            <end position="586"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR24120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="586" mass="64602" checksum="4197CF280C35919D" modified="2009-07-28" version="1">MPSTQSRSGANRPGLLSRGLKMLEIPVKDRQTLTVLRNPDILPIPAEDCTWSFFSYLAYWGVISFSVGTWVSANAALDVGLSYAETIGTFIVGDAVTIVFTLANSYHGLDWRVGYTLSQRFVFGIYGSGLGILVRILMSIVNYGSNAWLGGLCVNMILDSFSHHYVHLPNTLTHKVAMSTKELIGFVLFHVITAFFYLMKPKSMNYFLIWSCVATCFGMMGMVIYLTKINGGVGSAFRESETTIHGSDRAWMWVYMVSYWFGSVSPGSTNQSDYSRFASNKTGLYLGVIVALLVPTTVVPVFGVIGASTTSQLYGDAIWTPMDIFNYWLKENYSAGARAASFFCGVAFTASQISYTISNCGFASGMDLSGVLPQYINIFRGAIFTALVSVAVQPWNFYNKDSNTFLSVMSSFGVVMTPIISVMIADNFLVRKRKYSVSQAFVLKGEYYYTLGTNWRAMAAFVCGMAPGLPGIAAEANANIMAKMDRGIVNFFYADSFTSFAISFFMYWILCLIFPVKIGIEQDDKDYFGAFTDEEARERGVVPFSELTPEEIEEHNFSLGALDTPHSSSASSTGVDAHYEGGEKTK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-11-03" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKX0</accession>
    <name>COQ4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ubiquinone biosynthesis protein COQ4, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>4-hydroxy-3-methoxy-5-polyprenylbenzoate decarboxylase</fullName>
            <ecNumber evidence="1">4.1.1.130</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Coenzyme Q biosynthesis protein 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">COQ4</name>
        <name type="ordered locus">KLTH0F08184g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Lyase that catalyzes the C1-decarboxylation of 4-hydroxy-3-methoxy-5-(all-trans-polyprenyl)benzoic acid into 2-methoxy-6-(all-trans-polyprenyl)phenol during ubiquinone biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 4-hydroxy-3-methoxy-5-(all-trans-polyprenyl)benzoate + H(+) = a 2-methoxy-6-(all-trans-polyprenyl)phenol + CO2</text>
            <dbReference type="Rhea" id="RHEA:81179"/>
            <dbReference type="Rhea" id="RHEA-COMP:9551"/>
            <dbReference type="Rhea" id="RHEA-COMP:10931"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:62731"/>
            <dbReference type="ChEBI" id="CHEBI:84443"/>
            <dbReference type="EC" id="4.1.1.130"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of a multi-subunit COQ enzyme complex, composed of at least COQ3, COQ4, COQ5, COQ6, COQ7 and COQ9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">This protein may be expected to contain an N-terminal transit peptide but none has been predicted.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the COQ4 family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.130" evidence="1"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554558.1">
        <property type="nucleotide sequence ID" value="XM_002554512.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKX0"/>
    <dbReference type="SMR" id="C5DKX0"/>
    <dbReference type="FunCoup" id="C5DKX0">
        <property type="interactions" value="554"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKX0"/>
    <dbReference type="GeneID" id="8292764"/>
    <dbReference type="KEGG" id="lth:KLTH0F08184g"/>
    <dbReference type="eggNOG" id="KOG3244">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061241_0_2_1"/>
    <dbReference type="InParanoid" id="C5DKX0"/>
    <dbReference type="OMA" id="YYERHFH"/>
    <dbReference type="OrthoDB" id="4249at2759"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031314">
        <property type="term" value="C:extrinsic component of mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120539">
        <property type="term" value="F:4-hydroxy-3-methoxy-5-polyprenylbenzoate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03111">
        <property type="entry name" value="Coq4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007715">
        <property type="entry name" value="Coq4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027540">
        <property type="entry name" value="Coq4_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12922">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12922:SF7">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS PROTEIN COQ4 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05019">
        <property type="entry name" value="Coq4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0496">Mitochondrion</keyword>
    <keyword id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0831">Ubiquinone biosynthesis</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000388117" description="Ubiquinone biosynthesis protein COQ4, mitochondrial">
        <location>
            <begin position="1"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03111"/>
        </source>
    </evidence>
    <sequence length="327" mass="38006" checksum="A58A170FC65EBAE3" modified="2009-07-28" version="1">MIRTSFKHSIETRNQLLHRRHFVVAAALTVGNFVFGRDARLADAMDNGELHNKNEDYKAKAEELKEQRLRSIANSRPMKPCYEGHVPLYPHERVLLFITSGLKSYFHPEDGENIVQLGEASAFTPFLERLKNTMLSDKTGRRILREQPDITSESLDMDRLKKMAPNTLGHSYYKWLIKEGVSPDTRAPVKYIDDPTHAYIFKRYRQCHDFYHAVNDLPIIIEGEIAVKALEAANIGVPMAALGALLAPLRLKPVQKKRLYDVYLPWAVRTGLSCEPLINVYWEELLEHDVEELRKKLRITPPPDLRAIRRERAQQRKQFKLKYERHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DL93</accession>
    <name>C5DL93_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0F11044p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0F11044g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554681.1">
        <property type="nucleotide sequence ID" value="XM_002554635.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DL93"/>
    <dbReference type="FunCoup" id="C5DL93">
        <property type="interactions" value="144"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DL93"/>
    <dbReference type="GeneID" id="8292894"/>
    <dbReference type="KEGG" id="lth:KLTH0F11044g"/>
    <dbReference type="eggNOG" id="KOG0800">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071677_0_0_1"/>
    <dbReference type="InParanoid" id="C5DL93"/>
    <dbReference type="OMA" id="QEINICC"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="4"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR24244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="291" mass="33528" checksum="66D9E7BA7C4127BD" modified="2009-07-28" version="1">MDSCPICFESLSKGACRLVECGHKYHFNCIRRWHYHSKNLQCPTCRIKSRHLEDIERKVTINLAEFSDANLAISEISAQMGSLEIRSNEVRQEETRWNLDSLECRLCGESNVRTNVFCQECHTAYHEICLRTLHLEVGDFSRCVACCNCHHIFGSSSREPPASREHLARFLTERPGLHAARTHPHRSIRAPTSDGFEVVQSWRLLSELQEKCCLLDLSEHKRKIQAHVRKVLNAHYSKGSGAIATKDQYTEINKAVSRNLYRVSDNKYQPDKFDYDVEALRLIAIELRKTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLM0</accession>
    <name>C5DLM0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0G01760p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0G01760g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Golgi apparatus membrane</location>
            <topology evidence="7">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555118.1">
        <property type="nucleotide sequence ID" value="XM_002555072.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLM0"/>
    <dbReference type="FunCoup" id="C5DLM0">
        <property type="interactions" value="900"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLM0"/>
    <dbReference type="GeneID" id="8293376"/>
    <dbReference type="KEGG" id="lth:KLTH0G01760g"/>
    <dbReference type="eggNOG" id="KOG0087">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041217_23_0_1"/>
    <dbReference type="InParanoid" id="C5DLM0"/>
    <dbReference type="OMA" id="ITAIYQM"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034498">
        <property type="term" value="P:early endosome to Golgi transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01868">
        <property type="entry name" value="Rab11_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000067">
        <property type="entry name" value="ras-related protein RABA1f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0636">Prenylation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="189"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR24681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="218" mass="24257" checksum="30FCF615906D9C89" modified="2009-07-28" version="1">MNNEDYGYDYDYLFKIVLIGDSGVGKSNLLSRFTTNEFNMESKSTIGVEFATRTIEVEGKKIKAQIWDTAGQERYRAITSAYYRGAVGALIVYDISKSSSYENCNHWLTELRENADENVAVGLIGNKSDLAHLRAVPTDEAKNFAQENQLLFTETSALNSENVDQAFRELITAIYQMVSKHQVDLNEYGNNAGQAPKGPTISLTPAPAKKNKKSDNCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLX0</accession>
    <name>C5DLX0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">KLTH0G04180p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0G04180g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PIGS family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555218.1">
        <property type="nucleotide sequence ID" value="XM_002555172.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLX0"/>
    <dbReference type="FunCoup" id="C5DLX0">
        <property type="interactions" value="814"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLX0"/>
    <dbReference type="GeneID" id="8293484"/>
    <dbReference type="KEGG" id="lth:KLTH0G04180g"/>
    <dbReference type="eggNOG" id="KOG2459">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010026_1_0_1"/>
    <dbReference type="InParanoid" id="C5DLX0"/>
    <dbReference type="OMA" id="AEHKYAV"/>
    <dbReference type="OrthoDB" id="28748at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042765">
        <property type="term" value="C:GPI-anchor transamidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016255">
        <property type="term" value="P:attachment of GPI anchor to protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019540">
        <property type="entry name" value="PtdIno-glycan_biosynth_class_S"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21072">
        <property type="entry name" value="GPI TRANSAMIDASE COMPONENT PIG-S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21072:SF13">
        <property type="entry name" value="GPI TRANSAMIDASE COMPONENT PIG-S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10510">
        <property type="entry name" value="PIG-S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="462"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="492"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR24781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="518" mass="58910" checksum="11AE4B510D5677D3" modified="2009-07-28" version="1">MSNLWLRRAVAFWFVAVFILAGLPAWYKLTTIYRAPLPSEYIRSIHQNMHSDVHIVIPVYVRSDTYRFPDVHDAIQVQVNELLRSRPSHVQWSLQVLEYDESVVDQDSDYVATLVLDDFAGFTLAYDSKETLVFFNNEAVMANDVPFFAAQTLVEHTFGAEWEEFSGSRPEKNTGSSEMAISYSPNVHLSISLLTGDGRPIGWDIDTTLDNYFTPLRRFLSPLVNFTVDTSIVQFNDLNVGSIHSGENTTWQDLAHTIDLSELSSMNYYKEKSVLNLAIVFPSLEAGELSFVNATSQRPWQTFMVPRWGSLVLNKAPLPDNVRLTEEYLAPVIYNFARELFQFLGLATQSADLTTPYATIDSFKRVTIIKNLEHGAETLWSLTKLTEQFPQMSVPKQVLSDVEKALDLRLQLIELLNNPEKGGDRTWNQALLLSNEFVRVCERAFFHKEMVQQNFFPQEHKIAVYLPLLGPLTVIILSGLLNALREKEGPHVANNSLTEKERLQDDDTTVDSSERSAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5DM24</accession>
    <name>C5DM24_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">serine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Seryl-tRNA synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Seryl-tRNA(Ser) synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0G05368g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.1.1.11" evidence="1"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555272.1">
        <property type="nucleotide sequence ID" value="XM_002555226.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DM24"/>
    <dbReference type="FunCoup" id="C5DM24">
        <property type="interactions" value="921"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DM24"/>
    <dbReference type="GeneID" id="8293538"/>
    <dbReference type="KEGG" id="lth:KLTH0G05368g"/>
    <dbReference type="eggNOG" id="KOG2509">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023797_4_3_1"/>
    <dbReference type="InParanoid" id="C5DM24"/>
    <dbReference type="OMA" id="EQNCIDR"/>
    <dbReference type="OrthoDB" id="10264585at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004828">
        <property type="term" value="F:serine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006434">
        <property type="term" value="P:seryl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002317">
        <property type="entry name" value="Ser-tRNA-ligase_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010978">
        <property type="entry name" value="tRNA-bd_arm"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00414">
        <property type="entry name" value="serS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11778">
        <property type="entry name" value="SERYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001529">
        <property type="entry name" value="Ser-tRNA-synth_IIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00981">
        <property type="entry name" value="TRNASYNTHSER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46589">
        <property type="entry name" value="tRNA-binding arm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="11">
        <location>
            <begin position="155"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="46"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="280"/>
            <end position="282"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="296"/>
            <end position="299"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="367"/>
            <end position="370"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="401"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for serine binding" evidence="8">
        <location>
            <position position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001529-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001529-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR24835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="442" mass="49761" checksum="01C8487A5F5B3154" modified="2009-07-28" version="1">MQLRALSTTACRALRKPQFDVKGISGRLEAYLHSIKARQLLASPQLSAELQNLPKQENELKLLNQQFARIQTARKDIEREIKEDKSKMLQSRHRLQELKTEAQAVAADLKHMQDKVQETCMSLPNLIHGDVPLTEPQVLQWLSPQSEFRPDPKRDHHDIMVRKELVDFRSAATVTGNSWYYLINDGALLEQALVSYATKKARDRGFIMCNPPSIAKNEVIDACGFRPRDMNGEQQIYHLADTQWGLTATAEIALAGLGIDAVLDLSRGSKKLCGVSRSYRAEAGARGKDTRGLYRVHEFTKVELFCWARPDDSDQMLEELRQFQTELVEELGLPAKVLNMPANDLGAPAYRKYDIEVWMPGRGSFGEITSTSNCTDFQSRRMNTKFKDPVTGKSSHVHTLNGTAMAVPRVIMALVENFYDPVSNLIAVPAPLVPYMDGKTHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DME4</accession>
    <name>C5DME4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0G08162p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0G08162g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555392.1">
        <property type="nucleotide sequence ID" value="XM_002555346.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DME4"/>
    <dbReference type="FunCoup" id="C5DME4">
        <property type="interactions" value="1460"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DME4"/>
    <dbReference type="GeneID" id="8293663"/>
    <dbReference type="KEGG" id="lth:KLTH0G08162g"/>
    <dbReference type="eggNOG" id="KOG1763">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042870_1_0_1"/>
    <dbReference type="InParanoid" id="C5DME4"/>
    <dbReference type="OMA" id="GREMFYF"/>
    <dbReference type="OrthoDB" id="278280at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.400.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032378">
        <property type="entry name" value="ZC3H15/TMA46_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12681:SF0">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12681">
        <property type="entry name" value="ZINC FINGER-CONTAINING PROTEIN P48ZNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16543">
        <property type="entry name" value="DFRP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="85"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="156"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="85"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="156"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="44"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR24955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="341" mass="39263" checksum="7413A6A35033A811" modified="2009-07-28" version="1">MPPKKKQQQPVKKKDNVDKTFGMKNKNRSTKVQKFIQQVQTQVDPKKEEMKRKKQEEKLLRERQEAERRALFNPAMDQSVKSGVDPKTVLCAMFKIGNCNKGARCKFSHDPNVGRRVEKKDLYQDARAEKEADTMDQWDEEKLRSVILSKHGNLRTTTDKVCKFFIEAVENGKYGWFWVCPNGGDKCMYRHSLPEGFVLKTKEQKRLEKEALDSQPKITLEEFIETERDKLDKSKLTPITVANFAKWKQDHITQKINAEKRDEAKRKPTGREVVLKKFTEDKKFAEVDDMDATKGSAWDLSEFTRALKDAEANTGDGIKDYGDGSNPTFDLVNKTTTTASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMF1</accession>
    <name>C5DMF1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="20">KLTH0G08360p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="ordered locus">KLTH0G08360g</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">May play a role in membrane trafficking.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the tricalbin family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555399.1">
        <property type="nucleotide sequence ID" value="XM_002555353.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMF1"/>
    <dbReference type="FunCoup" id="C5DMF1">
        <property type="interactions" value="177"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMF1"/>
    <dbReference type="GeneID" id="8293672"/>
    <dbReference type="KEGG" id="lth:KLTH0G08360g"/>
    <dbReference type="eggNOG" id="KOG1012">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001661_1_1_1"/>
    <dbReference type="InParanoid" id="C5DMF1"/>
    <dbReference type="OMA" id="GWTGDVM"/>
    <dbReference type="OrthoDB" id="1029639at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090158">
        <property type="term" value="P:endoplasmic reticulum membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061817">
        <property type="term" value="P:endoplasmic reticulum-plasma membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035621">
        <property type="term" value="P:ER to Golgi ceramide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055091">
        <property type="term" value="P:phospholipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060304">
        <property type="term" value="P:regulation of phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd04044">
        <property type="entry name" value="C2A_Tricalbin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04052">
        <property type="entry name" value="C2B_Tricalbin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04045">
        <property type="entry name" value="C2C_Tricalbin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04040">
        <property type="entry name" value="C2D_Tricalbin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21678">
        <property type="entry name" value="SMP_TCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000217">
        <property type="entry name" value="Tcb1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000226">
        <property type="entry name" value="Tcb1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000230">
        <property type="entry name" value="Tcb1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037761">
        <property type="entry name" value="C2A_Tricalbin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037765">
        <property type="entry name" value="C2B_Tricalbin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037762">
        <property type="entry name" value="C2C_Tricalbin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037756">
        <property type="entry name" value="C2D_Tricalbin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031468">
        <property type="entry name" value="SMP_LBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056910">
        <property type="entry name" value="TCB1-3_C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017147">
        <property type="entry name" value="Tricalbin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052455">
        <property type="entry name" value="Tricalbin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46980">
        <property type="entry name" value="TRICALBIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46980:SF2">
        <property type="entry name" value="TRICALBIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24920">
        <property type="entry name" value="C2_TCB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037232">
        <property type="entry name" value="Tricalbin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51847">
        <property type="entry name" value="SMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="12" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="104"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="127"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="SMP-LTD" evidence="19">
        <location>
            <begin position="169"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="18">
        <location>
            <begin position="363"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="18">
        <location>
            <begin position="509"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="18">
        <location>
            <begin position="637"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="18">
        <location>
            <begin position="967"/>
            <end position="1090"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="808"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1157"/>
            <end position="1176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="811"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1160"/>
            <end position="1176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00059313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00061174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CAR24962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1176" mass="131110" checksum="4EC86CFBEEE11810" modified="2009-07-28" version="1">MATTGSEVVLPTERDQVAVVDGVDKLAPPQANEEKVSKNVSEDHTKNVNASEFERPTVDASYVGWKQIGGWEEKDELTLEDELMDLNNETFLDNVIPDKFYGDWYQTAGVIFLGGSLSFLIGKLGFSLGPVFFVVLAMSLLYRASIKRYRATIRDQVQKEFTVQKVEGDYESMEWLNSFLDKYWARLEPEVSQMIVQQVNEILATNPAIPAFVKALWIDKFTLGVKPPRIDLVKTYQNTDTDVVVMDWGVSFTPHDLSDLNSKQLKNYVNQKVTINAKAFGLPLSVSVSDIAFKAMLKVRFKLMTPFPHIETVNLQLTETPDVDFVAKLLGESIFNWEILSIPGLYPLIRELAKKYMAPILMPPFSLQLNIPQLISGSAVSIGILEVTVKDAIDIKRARNILNRSVDPYLSFEFNGVCVGKTRTVRDSLNPVWNETLFLLLNSFTEPLSIVLYDRRENVKDKVLGRIEHNLSTLHDENTQKNLSGCFLRSSKPVGQLNFGLKFHPTLEPKQLPDGTVEDAPDLNVGISKLIIEEAKELSEPGSKVSAYVELYVNAKLVLTTGSVKNTETPSWFQEYEAVIADRRKTRTKIVIKNAKGETIGSTVQTLSDLIDRSEVGKCWIPLQNGKGQIKVTTYWKPVELGMGANSVAYTPPIGAVRVFISKAEGLRNLEKFGKIDPYARVLVNGIPRGRTDVRGSTLNPVWNQGIYVAVTSPNQRITLECLDVETVGADRTLGKFNIDVANMFQKGNDDKYVENVDEEPKVGRLVDKKGAKGSVTYYVSFYPTVPVLSLEEIQEVDELNERKKRLQIQESEKSDDELSKQAEQKRKEEEFEVKELEDLFSNKLKLDLDELLQYKSGVFAFSVLGGELPQTGCYVQAFFDSNGYPRYVSQKFGGRTIRNGATGDVIIKELDWSITTFRVTKKGDASKAEECYAEVTIPTIELVKNCYYKPSILNLTGGNSAKLMLQVSWFPVAASKLPQADLITNSGDLTINVLGANNLISADRNGKSDPFVKFYIDNNESSFFKTHHKKRTLDPVWNEKCEVQINNRVNNYLKIKMMDWDAGNKDDLIGEAIYPLASVDPENPSDVDIPLIGPEGKDGGVLHLSFSFSPRYTLSVRKTEKKVGDLASKGISSGLHAGTSVIGGGLGAVGKIKKGIFGGDKKDDKKSDKKDGDAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMG8</accession>
    <name>C5DMG8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0G08800p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0G08800g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555416.1">
        <property type="nucleotide sequence ID" value="XM_002555370.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMG8"/>
    <dbReference type="FunCoup" id="C5DMG8">
        <property type="interactions" value="96"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMG8"/>
    <dbReference type="GeneID" id="8293692"/>
    <dbReference type="KEGG" id="lth:KLTH0G08800g"/>
    <dbReference type="eggNOG" id="ENOG502SAJ9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_598599_0_0_1"/>
    <dbReference type="InParanoid" id="C5DMG8"/>
    <dbReference type="OMA" id="GNFIFHR"/>
    <dbReference type="OrthoDB" id="4036037at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="185"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="433"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="197"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="448"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="457" mass="51954" checksum="6DB48D02F9403A13" modified="2009-07-28" version="1">MCSEYSLLSQESIVVDLSIQNPRLSNALHCYPTSTSPSSPDSDRLPFYQRTNAARALEDAEDMTDEEQEQSLIQPLIDAHNRSSDEPLRLKTTCATTALPQRVPVPYRTHRPQHSQTVTILDESESEQSKPFVSQQLTMSQFDLQQTRNFEQRAKLRQQALEAHNSDSNNTLGILDKVCKWSRYSKRAQKRPQRTPSARSASSVRRAPSLSKRKDFSNKSEMASFASSGNFIFHRGFSLRRAPGLPPRYKYRSRAELASAMDYVAPESTPVTDFTTSQHFLTMQLKPPAFWQVLRLHPKFTYKFVSAGSLYRQSLNSQKGLHRNSIRRPSPLPQRVRTLRRSKSVPGSRKNITLQLQNPQLYDVWRHYVMSVVLQRIQFRMYLVNSSNSDKVSTSESDASQPLSRCSSLALQASWINDFGARNTRGDDTYAGSCKDEASSISSSGRHQGIRRLRLVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMI4</accession>
    <name>C5DMI4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="18">KLTH0G09196p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">KLTH0G09196g</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555432.1">
        <property type="nucleotide sequence ID" value="XM_002555386.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DMI4">
        <property type="interactions" value="1200"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMI4"/>
    <dbReference type="GeneID" id="8293709"/>
    <dbReference type="KEGG" id="lth:KLTH0G09196g"/>
    <dbReference type="eggNOG" id="KOG0386">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000315_15_0_1"/>
    <dbReference type="InParanoid" id="C5DMI4"/>
    <dbReference type="OMA" id="VNYISHT"/>
    <dbReference type="OrthoDB" id="5857104at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016586">
        <property type="term" value="C:RSC-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140008">
        <property type="term" value="F:histone H4 reader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd04369">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17996">
        <property type="entry name" value="DEXHc_SMARCA2_SMARCA4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000073">
        <property type="entry name" value="Chromatin structure-remodeling complex subunit rsc4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000008">
        <property type="entry name" value="Chromatin structure-remodeling complex subunit snf21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000843">
        <property type="entry name" value="Chromatin structure-remodeling complex subunit snf21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014012">
        <property type="entry name" value="HSA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029295">
        <property type="entry name" value="SnAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10799">
        <property type="entry name" value="SNF2/RAD54 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07529">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14619">
        <property type="entry name" value="SnAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01314">
        <property type="entry name" value="SnAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51204">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 12" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="7" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HSA" evidence="17">
        <location>
            <begin position="298"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="15">
        <location>
            <begin position="473"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="16">
        <location>
            <begin position="786"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="14">
        <location>
            <begin position="1220"/>
            <end position="1290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="117"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1073"/>
            <end position="1197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="127"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="1075"/>
            <end position="1085"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="1136"/>
            <end position="1147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="1151"/>
            <end position="1160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAR24995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1308" mass="149667" checksum="3EBB8BE1154EE956" modified="2009-07-28" version="1">MNTAEIDVVMPSAASQGEAEKVTVNIPAPETLAQLDQLLGRYRAVIQEPEKNKVEIDALERVLCSISEQQDEYTVKLQKLRTGMQNQPAYDEQLFKSQLIALQLLSRDVDVPDGLLTEEQTEDDRTVGSSNHTSTLESTESNLKPVKLSLDFDSNAKSFGLYDKFSNLGMPKGKESLGAISVEGTILSRIAERIKELESLPANLGTYSLEDSLLFTAKDDVPSSIDDLKLKAIVELRALKLLTKQKSLRQKLIMNVTSQAHHTIPSLRDSPFTMAAQRSVQVRNKVIVPQTARLAEELERQQLLEKRRKERNIHMHKVNVIIDYVQDQQVTHTSPRERGAQFGKLCLSLHNQTEKDEQKRVERTAKQRLAALKSNDEEAYLKLLDQTKDTRITHLLRQTNSFLDSLAQAVRVQQNEAKIREGGEIRPMTDEEREKIDYYEVAHSVKEKVEKQPSILVGGTLKEYQVRGLEWMVSLYNNHLNGILADEMGLGKTIQSISLITYLKEAKSEPGPFLVIVPLSTITNWTLEFEKWAPSLATVVYKGTPNQRKSMQHQIRTGNFEVLLTTYEYIIKDRSLLAKHDWSHMIIDEGHRMKNAQSKLSFTLTRYYRTRNRLILTGTPLQNNLPELWALLNFVLPKIFNSAKTFDEWFNTPFANTGGQEKLELTEEETLLVIRRLHKVLRPFLLRRLKKEVEKDLPDKVEKVVKCKLSGLQHQLYQQMLKHNALFFGAGTEGATKGGIKGLNNKIMQLRKICNHPFVFDEVEGIINPTRGNSPLLYRVAGKFELLDRILPKFKATGHRVLMFFQMTQVMDIMEDFLRMRDLKYLRLDGATKTEERTGMLKLFNAPDSEYFCFLLSTRAGGLGLNLQTADTVIIFDTDWNPHQDLQAQDRAHRIGQKNEVRILRLITTDSVEEVILERALQKLDIDGKVIQAGKFDNKSTAEEQEAFLRRLLENENVKDENDEAELDDEELNEILARGDDERKLFDKMDEERAAMELKQAKSQGLSTPLPRLIQLDELPEVLTEDITNHLQTEPAAVGRIRERKKVYYDDGLTEEQWLQAVDNDEDSLEDAINRKRRSRERRQHSKELKDSEPGEIDTLDNSLEPTPEIELAKPNGKRSRAARKAAASEVKNENEQDEQLEEEEEDGHTPKKLKLKVKLSLKSDQQKSTPGELDGPTVEQPQTSAPKPKSKSKKVTTCEFTPQVEHLLEEMRAQIDPTDEHARTSVFEILPSKKLYPDYYKLISNPVALDTITKKCKKGLYGSLEDVRQDLETMFGNAQFYNEEGSWIFNDAEELRKFVSAWFESNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMJ4</accession>
    <name>C5DMJ4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0G09438g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Substrate-binding subunit of tRNA (adenine-N1-)-methyltransferase, which catalyzes the formation of N1-methyladenine at position 58 (m1A58) in initiator methionyl-tRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the TRM6/GCD10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555442.1">
        <property type="nucleotide sequence ID" value="XM_002555396.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMJ4"/>
    <dbReference type="FunCoup" id="C5DMJ4">
        <property type="interactions" value="1008"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMJ4"/>
    <dbReference type="GeneID" id="8293720"/>
    <dbReference type="KEGG" id="lth:KLTH0G09438g"/>
    <dbReference type="eggNOG" id="KOG1416">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010916_2_0_1"/>
    <dbReference type="InParanoid" id="C5DMJ4"/>
    <dbReference type="OMA" id="TRCRPYQ"/>
    <dbReference type="OrthoDB" id="10254665at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031515">
        <property type="term" value="C:tRNA (m1A) methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017423">
        <property type="entry name" value="TRM6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12945">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12945:SF0">
        <property type="entry name" value="TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASE NON-CATALYTIC SUBUNIT TRM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04189">
        <property type="entry name" value="Gcd10p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038170">
        <property type="entry name" value="tRNA_m1A_mtfrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="66"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="465"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR038170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR25005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="487" mass="54468" checksum="CE162BCFF20713E7" modified="2009-07-28" version="1">MDPLKHITYYNHVLVRLPSNNLKVCELKPNSDVSLGKFGAFKVNDIIGYPFGTTFEIHYDNAIEASAPDAGPSGDAAQEGTSSKGARNKFKIPVGRLSVIESRLTANAAATGLGSEEEGTPQRELQLVGNSDSNKNLINIGSSIQKLSSEEIEQLKQQSVSSNEIISKMIEAHGSFHQKTVYSQEKYLKRKKQKFAKFFTVEYMSSSGLLQYLIDKGDVLRAMDLSEESMGMILNLANIRSNGTYLCVDETGGLLVYALLERMFGGENDSAAQGKIVVVHENEHPNLDLLKFSNYTEEFIQQHVKTVSILDYFEPPVTEEVKEAFTPLTDEQLKSLKSNKKGAYYRRLKWYNTQLEIIEWATKQQYDGLVVASTLHLPTFVPKLGERVHGSRPIVCYSQFKETVLELSHTLYNDLRYLAPTIVETRCRPFQTIRGRLHPVMTMRGGGGYMMWCHKVIPVGAADAALQPRPEEAPQGAEAESKKQKTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMR0</accession>
    <name>C5DMR0_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="7">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0G10934g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="7"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555508.1">
        <property type="nucleotide sequence ID" value="XM_002555462.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMR0"/>
    <dbReference type="FunCoup" id="C5DMR0">
        <property type="interactions" value="73"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMR0"/>
    <dbReference type="MEROPS" id="C19.002"/>
    <dbReference type="GeneID" id="8293787"/>
    <dbReference type="KEGG" id="lth:KLTH0G10934g"/>
    <dbReference type="eggNOG" id="KOG1867">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008279_6_1_1"/>
    <dbReference type="InParanoid" id="C5DMR0"/>
    <dbReference type="OMA" id="VECNRCA"/>
    <dbReference type="OrthoDB" id="2020758at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02662">
        <property type="entry name" value="Peptidase_C19F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF888">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="4 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="9">
        <location>
            <begin position="99"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="142"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="545"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="608"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="144"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="163"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="545"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="560"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="576"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="635"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="657"/>
            <end position="670"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR25071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="758" mass="85945" checksum="6DC012A0D08777CB" modified="2009-07-28" version="1">MNFVLEKLSALFSTLSIFVRNLSHIDNKSSTLIVLIASTGILLYKNKRIFRNIMENIGTVGGDLTRSFRNNYKGSMFNKWGSKDPEQEAAMLKRGGYVGGLVNDGNTCFMNSVLQSLASSKTLLQFLDNEVIQAYKEAEESEDEHVAEKEFESEKKLSESKTKPKKKVYGKRKKRLTRNAEKDALDDDQDANIEFSTTLKELLNKLNAKHYRDRPYFKTNKLLKTMSKAPNKSIILGYDQEDAQEFFQTILSELEKNVKSLYGKSSKDEEAPVQFDQLPEDSMVGQERLGEMGTVYIPTEQIDPNCVLAHENKSLYSPFKLITPLDGLTAERIGCLQCGENGGIRYSVFSGLSLNLPSENLGSTLKLSELLREWIKPEIIEGVECNRCALNAVLEHLEKTLIQYEGSEHSSEKLVAAVKARVEQLKDILAKPAIDDDDYKKLHTENMVRKCSKSKQIIISRPPPLLCIHINRSVFDPRTYMIRKNNSRVLFKSKLNLAPWCCAPDEINLDARLPMSKKEQKSLESSEDENVGGEYFAKLHRHFEEEFEDSEEEEGNFEASNRDVSDYDPLRGEIESSSDENESSSDGEYEVDALGNRVFKQNGAQTEQELVDGTSADPEVASLEGSDAAERLDGIEPETLDNEDEEDSESDHEVKQPPSLSSLHHAPSSSTVPVGPLTYALRSVIVHYGTHNYGHYIAFRKFRGLWWRISDETVYVVDEAEVLSTPGIFMLFYEHDYNESLHAMNDDLEWEEENEDRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5DN19</accession>
    <name>C5DN19_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0G13464p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0G13464g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555617.1">
        <property type="nucleotide sequence ID" value="XM_002555571.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DN19"/>
    <dbReference type="FunCoup" id="C5DN19">
        <property type="interactions" value="44"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DN19"/>
    <dbReference type="GeneID" id="8293900"/>
    <dbReference type="KEGG" id="lth:KLTH0G13464g"/>
    <dbReference type="eggNOG" id="KOG0254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_22_3_1"/>
    <dbReference type="InParanoid" id="C5DN19"/>
    <dbReference type="OMA" id="VPHEHQA"/>
    <dbReference type="OrthoDB" id="3437016at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015174">
        <property type="term" value="F:basic amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF191">
        <property type="entry name" value="VACUOLAR BASIC AMINO ACID TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="85"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="153"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="214"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="239"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="279"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="311"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="349"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="389"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="413"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="445"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="88"/>
            <end position="585"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR25180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="593" mass="64026" checksum="8600DD633BF2F7FA" modified="2009-07-28" version="1">MVQSEHSPLLTPVDRDAMQDPSTDLPGFVANQIEQQDHLLDIEQGRAYGSTTKSSISSENEREDAGGATVAGCGSAEFYLPSSRILIIMFSMFLGIFLAALDGTIVSTLLTHIGSEFNSLDKASWIATSYLLSSSTCQPLYGKLSDRLGRKPLLVFSNAVFAIGCLICGLSNSIWAVVAGRFISGIGGCGLTSLATMIMSDLVPLRDRAVYQGLCNFVFGLGTASGGLVGAYFRDWRLAFILQCPISVLSGILIVVYLELPPRASASNKDCWSARLRRLDWAGATSLIAFLFLFMLTSSMGGSSVAYGSRLFAAMCAATLVFGAVFVYVELRVAADPVLPVTFLRDRSVLGSSLANWFCMMSMMTTNFYLPVYWSSVLGMKATDVGKRAMPSFFSVAFGSLGAGLYMKRTGKYYYFLLAFCVLAIAGQVQIVLITPQMPVWRQYLLQVVPGFGVSVLITVTLLAMIAAVPHEHHAATTSISYAFRSTGATLGVSVGAAVFRSSLTSLLDAKVMRFASDGHSERELRGIIASAARSSDWVHRSAPPFVRATLLECYHFASRATFKFCLTTMVLAGVSCSIIKEHKLHTSIKRGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DND4</accession>
    <name>C5DND4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0G16060p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0G16060g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutaredoxin family. Monothiol subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555732.1">
        <property type="nucleotide sequence ID" value="XM_002555686.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DND4"/>
    <dbReference type="FunCoup" id="C5DND4">
        <property type="interactions" value="25"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DND4"/>
    <dbReference type="GeneID" id="8294018"/>
    <dbReference type="KEGG" id="lth:KLTH0G16060g"/>
    <dbReference type="eggNOG" id="KOG1752">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026126_0_1_1"/>
    <dbReference type="InParanoid" id="C5DND4"/>
    <dbReference type="OMA" id="VAQNANF"/>
    <dbReference type="OrthoDB" id="423313at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005796">
        <property type="term" value="C:Golgi lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004362">
        <property type="term" value="F:glutathione-disulfide reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03419">
        <property type="entry name" value="GRX_GRXh_1_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000093">
        <property type="entry name" value="Glutaredoxin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011899">
        <property type="entry name" value="Glutaredoxin_euk/vir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014025">
        <property type="entry name" value="Glutaredoxin_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02180">
        <property type="entry name" value="GRX_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45694">
        <property type="entry name" value="GLUTAREDOXIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45694:SF5">
        <property type="entry name" value="GLUTAREDOXIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00160">
        <property type="entry name" value="GLUTAREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="domain" description="Glutaredoxin" evidence="5">
        <location>
            <begin position="111"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR25295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="213" mass="23454" checksum="5410DD9842D57814" modified="2009-07-28" version="1">MLSKRNLRVFVFTGVLLVLIYFIIQNAHTTVTSGNSSLSGQTASGAVIHSKGNHKSSGEVDPSVDKEIQEIKNEVGIKGDDGASSGTDFTQEKEFDPAKEYQYILANRPMIVFSKSRCPFSKKLKDLLAKEFEFSPSYMVVELDKHEHGAELQKHIGSLTGRSTVPNVIINGVSRGGCDDFEKLQEKGELLSSLKTWCDKALTVSKKEKPSNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNJ9</accession>
    <name>C5DNJ9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0G17644p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0G17644g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555797.1">
        <property type="nucleotide sequence ID" value="XM_002555751.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNJ9"/>
    <dbReference type="FunCoup" id="C5DNJ9">
        <property type="interactions" value="169"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNJ9"/>
    <dbReference type="GeneID" id="8294090"/>
    <dbReference type="KEGG" id="lth:KLTH0G17644g"/>
    <dbReference type="eggNOG" id="KOG0234">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006383_0_2_1"/>
    <dbReference type="InParanoid" id="C5DNJ9"/>
    <dbReference type="OMA" id="VKTHVFH"/>
    <dbReference type="OrthoDB" id="267323at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003873">
        <property type="term" value="F:6-phosphofructo-2-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004331">
        <property type="term" value="F:fructose-2,6-bisphosphate 2-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006003">
        <property type="term" value="P:fructose 2,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006000">
        <property type="term" value="P:fructose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003094">
        <property type="entry name" value="6Pfruct_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013079">
        <property type="entry name" value="6Phosfructo_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606">
        <property type="entry name" value="6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606:SF39">
        <property type="entry name" value="6-PHOSPHOFRUCTO-2-KINASE_FRUCTOSE-2,6-BISPHOSPHATASE YLR345W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01591">
        <property type="entry name" value="6PF2K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000709">
        <property type="entry name" value="6PFK_2-Ptase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00991">
        <property type="entry name" value="6PFRUCTKNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="6-phosphofructo-2-kinase" evidence="4">
        <location>
            <begin position="69"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="339"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="377"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="339"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="349"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR25360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="549" mass="62781" checksum="2FFFCE05B8200C3B" modified="2009-07-28" version="1">MDSSVLEEVPIKDTLSVPGSSMGNHMARTRRRWSSSSADKRPKVSETTDGVHEDSKSDYISPGQLYSTESGRLFHAGKILIVLVGLPATSKTLLSVAITRYTKWLGVRTSSFHVSEYRRRMVNVDYDRFPNDYFSAKPQTAEGQALRTKILDQVLLDMLEFFKESKGQLAVYDALNILVEERTQLQKIFSERNIKVLFIESVMTDSEMIKHNVDMATKSADYADWPQKDAIHDYMHRLRVNRELYEEMTPKEQLSYVKYVNFGERLSVNNNHFGYLINKIVFFLMNLRNKKGCVYFARCGTSDNDKYVDDELLNAEGVKYSNLLTDLVLQTVNERRASQQPVSITPIGSASSPNSGLSSRSMSPSLSPLLATAASLRRTESTGNASMRQPASGDGGDEDSFVVWTAPRKRTHDTAKFFEERGITVRQRYQLQQLHPGLVADLTEDEVSQKFPEEYKEWKKDPYHYRFPRAESYHDLAVRMEPLLLEMERMRGDILIIGHESALRVLYGYLMACSCSDIPNLKFTRDELIEISFSPFENKVRRIPVVMNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNP4</accession>
    <name>C5DNP4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Tubulin beta chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">KLTH0G18766g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11 12">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4 12">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the tubulin family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555842.1">
        <property type="nucleotide sequence ID" value="XM_002555796.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNP4"/>
    <dbReference type="FunCoup" id="C5DNP4">
        <property type="interactions" value="1620"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNP4"/>
    <dbReference type="GeneID" id="8294137"/>
    <dbReference type="KEGG" id="lth:KLTH0G18766g"/>
    <dbReference type="eggNOG" id="KOG1375">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015718_1_1_1"/>
    <dbReference type="InParanoid" id="C5DNP4"/>
    <dbReference type="OMA" id="WVPRSVN"/>
    <dbReference type="OrthoDB" id="1662883at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000070">
        <property type="term" value="P:mitotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097435">
        <property type="term" value="P:supramolecular fiber organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02187">
        <property type="entry name" value="beta_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000006">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000002">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000009">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013838">
        <property type="entry name" value="Beta-tubulin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002453">
        <property type="entry name" value="Beta_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01163">
        <property type="entry name" value="BETATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00228">
        <property type="entry name" value="TUBULIN_B_AUTOREG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9 12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 12" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="14">
        <location>
            <begin position="47"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="15">
        <location>
            <begin position="246"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="429"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="429"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAR25405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="449" mass="50297" checksum="913AE237EBEE27EC" modified="2009-07-28" version="1">MREIIHISTGQCGNQIGAAFWETICGEHGLDFNGNYHGLDDIQKARLNVYFNEASSGKWVPRSVNVDLEPGTIDAVRNSRIGNLFRPDNHIFGQSSAGNVWAKGHYTEGAELVDSVMDVVRREAEGCDSLQGFQITHSLGGGTGSGMGTLLISKIREEFPDRMMATFSVVPSPKTSDTVVEPYNATLSVHQLVEHSDETFCIDNEALYDICQRTLKLNQPSYNDLNHLVSSVMSGVTTSLRYPGQLNSDLRKLAVNLVPFPRLHFFMVGYAPLTSIGSQSFRSLSVPELTQQMFDAKNMMAASDPRNGRYLTVAAFFRGKVSVKEVEDEMHKVQTRNSSYFVEWIPNNVQTAVCSVAPKDLDMSATFIGNSTSIQELFKRVGDQFAAMFRRKAFLHWYTSEGMEEIEFSEAESNMNDLVSEYQQYQEATVEDDEELEYADEQPLPENFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNQ4</accession>
    <name>C5DNQ4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0G18986p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0G18986g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555852.1">
        <property type="nucleotide sequence ID" value="XM_002555806.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNQ4"/>
    <dbReference type="FunCoup" id="C5DNQ4">
        <property type="interactions" value="561"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNQ4"/>
    <dbReference type="GeneID" id="8294147"/>
    <dbReference type="KEGG" id="lth:KLTH0G18986g"/>
    <dbReference type="eggNOG" id="KOG0024">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_11_5_1"/>
    <dbReference type="InParanoid" id="C5DNQ4"/>
    <dbReference type="OMA" id="CSVGRPN"/>
    <dbReference type="OrthoDB" id="3941538at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003939">
        <property type="term" value="F:L-iditol 2-dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006062">
        <property type="term" value="P:sorbitol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05285">
        <property type="entry name" value="sorbitol_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000068">
        <property type="entry name" value="Sorbitol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045306">
        <property type="entry name" value="SDH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43161">
        <property type="entry name" value="SORBITOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43161:SF9">
        <property type="entry name" value="SORBITOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="8">
        <location>
            <begin position="14"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR25415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="354" mass="38072" checksum="C2B750C3EAD6F058" modified="2009-07-28" version="1">MNETQEAIVLVQKGEIAVESKPVPEITDPHYVKLQIKKTGICGSDVHYYVAGAIGDFVVKKPMILGHESSGLVVEVGSEVSRVRVGDRVAIEPGVPSRYSDETKAGRYNLCPHMQFAATPPIDGTLVKYYLAPEDFLVKLPDHVSYEEGALVEPLSVGVHANKLAGVAFNQRVAVFGAGPVGLLTGAVARAFGASEVVYIDVFEHKLSLSSNFGGTQFVNSANIKDEDDLVKEIERVLGGARPDVVFDCTGAEICIRTGIKVCNSGGTYVQVGMGHDDVNFPIGAIGAKELKVLGCFRYAFGDYRDAVQLIASGDVNVKPLVTHRFKFEDAEAAYEFNIKHGSEVIKTIISGPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNR7</accession>
    <name>C5DNR7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0G19272p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0G19272g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555865.1">
        <property type="nucleotide sequence ID" value="XM_002555819.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNR7"/>
    <dbReference type="GeneID" id="8294160"/>
    <dbReference type="KEGG" id="lth:KLTH0G19272g"/>
    <dbReference type="eggNOG" id="ENOG502S6UH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068510_3_0_1"/>
    <dbReference type="InParanoid" id="C5DNR7"/>
    <dbReference type="OMA" id="AWINNDN"/>
    <dbReference type="OrthoDB" id="60822at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011893">
        <property type="entry name" value="Selenoprotein_Rdx-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02174">
        <property type="entry name" value="CXXU_selWTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36417">
        <property type="entry name" value="SELENOPROTEIN DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_1G05220)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36417:SF2">
        <property type="entry name" value="SELENOPROTEIN DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_1G05220)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10262">
        <property type="entry name" value="Rdx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR25428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="125" mass="14201" checksum="E45B85D7552F348F" modified="2009-07-28" version="1">MPYPKISIRFCTKCKWNLRSAWYLQELLQTFQSSLGEVSLIPGESGEFRVLGYKEHDQPEVVIWDRTVDGGFPDSKYLKQKVKQLLLDADDQDQLGAHITRKAQTKSLVDKADCSSSGQCLDCET</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2H4</accession>
    <name>C5E2H4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="15">KLTH0H04972p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0H04972g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Nucleoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the nucleoporin GLFG family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556097.1">
        <property type="nucleotide sequence ID" value="XM_002556051.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5E2H4">
        <property type="interactions" value="838"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2H4"/>
    <dbReference type="MEROPS" id="S59.002"/>
    <dbReference type="GeneID" id="8294411"/>
    <dbReference type="KEGG" id="lth:KLTH0H04972g"/>
    <dbReference type="eggNOG" id="KOG0845">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005908_0_0_1"/>
    <dbReference type="InParanoid" id="C5E2H4"/>
    <dbReference type="OMA" id="PMGKGLN"/>
    <dbReference type="OrthoDB" id="3797628at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044613">
        <property type="term" value="C:nuclear pore central transport channel"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044614">
        <property type="term" value="C:nuclear pore cytoplasmic filaments"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008139">
        <property type="term" value="F:nuclear localization sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000973">
        <property type="term" value="P:post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034398">
        <property type="term" value="P:telomere tethering at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1610.10:FF:000003">
        <property type="entry name" value="Nucleoporin SONB, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.690">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1610.10">
        <property type="entry name" value="Peptidase S59, nucleoporin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025574">
        <property type="entry name" value="Nucleoporin_FG_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037665">
        <property type="entry name" value="Nucleoporin_S59-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007230">
        <property type="entry name" value="Nup98_auto-Pept-S59_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036903">
        <property type="entry name" value="Nup98_auto-Pept-S59_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021967">
        <property type="entry name" value="Nup98_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23198:SF6">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP98-NUP96"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23198">
        <property type="entry name" value="NUCLEOPORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04096">
        <property type="entry name" value="Nucleoporin2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13634">
        <property type="entry name" value="Nucleoporin_FG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12110">
        <property type="entry name" value="Nup96"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82215">
        <property type="entry name" value="C-terminal autoproteolytic domain of nucleoporin nup98"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51434">
        <property type="entry name" value="NUP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="8" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="11" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0811">Translocation</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Peptidase S59" evidence="14">
        <location>
            <begin position="429"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="170"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="262"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="330"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="21"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="67"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="98"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="140"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="280"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR30235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1305" mass="142460" checksum="80C4944F216A231E" modified="2009-07-28" version="1">MFGSNSGGSSLFGGGLNTSTPTSKITQTDNAFQPRQKSASGSLFGDVSTSRLNGASTPSPSGGLFGNKNNTSASQEAPNALFGGNANQDSNSGGLFGNNTNSSSTPNTNSSAGVNTNAPTGGLFGSASQSANVGGLFNKPNANPSNSRPLFGGSTSNSLNANSNGGIFGNSASASKPGQANAPGLFSATSSIPSSTGNFSNTTFSGGQGATSFGGNGVMLSSGNQGSIQGSTAASNPYGLQFNAIAKSAGSMPESITTSLFKSNAHSNKDGGANNAQKTSGSSSSPASSLKSNGTLIGKLSSRLRNTRSNELTQGLFSPVRKPIFRQEIVPTSSTTESNLKDHSHFQRGLENSAKANRSEAADMRKLKIDTGRSAAKKMKLLSGQSTTTSTKILGSRDLHQDLEEPRSVLQKRETTEPELTGKELNDKTVGYWCSPTIEQLSRLPIKQLCSVSNFVIGRQGFGSISFDSDVDLSSYVSDLKTSLFGKIVIFHGNKTVEVYPDGTSKPPLGYGLNVPATITLEQIYAVGKNSRTPLKEHSAEEVQLLIKKLRRLKGMSFVSYNPLEGLWTFKVQHFSIWGLIDEDGTEKKEQEPSSEADHKQRKIAVPKHRSIAAKSCQAFDKQAMDETSMHPRGGLDIDGVAFGTLVQNEIESGDTEFNELVEEKQYEPSDVDPEDFEGMEVQTALGVANNWATQLELADDPYNSAFRSTNLQRTGNREASSLSQAFDETFNTNKSAKRTLRLSHIPKLAKFSKDSKLLCNSKKDVSGCTLVDVLAWDNESRCRISQTLEKNYINAKVNMRGSNGYPMVKSFSVTISEMEATLAVGEGHNIWKLFSILFDEKGISPKKDRKAELDELVKGKRYQLLCSWVVQEVSKSVLNRIESTQNPNESIFYQLIIGDIIGATKAAIKLKNPHLSALITLLNTNNPNVRFLASQQLRKWKALNRKVDLFVVKIYQLLSGQPFDQTCVVDLTRELSWLECLSVYLLFSDMNASSLEEFVAQFLEAHPTSSKPVSYEVIFSVLKFFCSEVRDERMLKRMRVSKNVFDDRAFWFLVQTLRFKKCCNFTSDQTDSITLQFQEQLEANELYGEALFTLLFVHSDVLAKQHIDDLIRRHVHFFSGASGRDILKRFQVPDSLIFQAIALRHKFDENYLAEAENLLEGGLVEDAVQTLLTNVAPEMILRSTTDKQSLKTLRDILDKVPSEFLDTSGRDLGVFSEYLNFALDGHTDLATLEKLIHALPPFYATYGHVSKISICCSVMAYAVSLAYLMTENAEAAASSMKDRLLELPLGEPEARYIRKNLKAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2P2</accession>
    <name>C5E2P2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Mediator of RNA polymerase II transcription subunit 31</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0H06578g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Mediator complex subunit 31 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556165.1">
        <property type="nucleotide sequence ID" value="XM_002556119.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2P2"/>
    <dbReference type="FunCoup" id="C5E2P2">
        <property type="interactions" value="149"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2P2"/>
    <dbReference type="GeneID" id="8294481"/>
    <dbReference type="KEGG" id="lth:KLTH0H06578g"/>
    <dbReference type="eggNOG" id="KOG4086">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_147521_0_0_1"/>
    <dbReference type="InParanoid" id="C5E2P2"/>
    <dbReference type="OMA" id="YLEYWCE"/>
    <dbReference type="OrthoDB" id="10257739at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1340">
        <property type="entry name" value="Mediator of RNA polymerase II, submodule Med31 (Soh1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038089">
        <property type="entry name" value="Med31_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008831">
        <property type="entry name" value="Mediator_Med31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13186">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05669">
        <property type="entry name" value="Med31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0010">Activator</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR30303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="129" mass="15030" checksum="E4B967DAEFE3CC5F" modified="2009-07-28" version="1">MEDGGIPENNGVEQPVPEDIPPTRFEVELEFVQCLANVPYVIFLLSQQHIWRDPKFRAYLKYLEYWCKPPYAQCIVYPNCLFMLKLLNGFMEAAEVNEDGLLEGLEDLPKVLQSQGAQWMNEMVERWSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2S4</accession>
    <name>C5E2S4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">KLTH0H07304p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0H07304g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the OXA1/ALB3/YidC family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556197.1">
        <property type="nucleotide sequence ID" value="XM_002556151.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2S4"/>
    <dbReference type="FunCoup" id="C5E2S4">
        <property type="interactions" value="664"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2S4"/>
    <dbReference type="GeneID" id="8294514"/>
    <dbReference type="KEGG" id="lth:KLTH0H07304g"/>
    <dbReference type="eggNOG" id="KOG1239">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029282_3_0_1"/>
    <dbReference type="InParanoid" id="C5E2S4"/>
    <dbReference type="OMA" id="FPMAIFM"/>
    <dbReference type="OrthoDB" id="2148490at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032977">
        <property type="term" value="F:membrane insertase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032979">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane from matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20069">
        <property type="entry name" value="5TM_Oxa1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001708">
        <property type="entry name" value="YidC/ALB3/OXA1/COX18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028055">
        <property type="entry name" value="YidC/Oxa/ALB_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428:SF66">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE PROTEIN OXA1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428">
        <property type="entry name" value="OXA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02096">
        <property type="entry name" value="60KD_IMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="113"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="187"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="285"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="Membrane insertase YidC/Oxa/ALB C-terminal" evidence="12">
        <location>
            <begin position="115"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="356"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="356"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR30335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="383" mass="42442" checksum="41914FF3C99E15DD" modified="2009-07-28" version="1">MFRNCVTRSARLAARSSSITWQPRLSGPSFIRFKSSVPQPGLNTEIQTQLPSLDEISGATDAAASLADQASQVVGEASNHIGYLSSIGLAKSWWWPPDLIQNIMEQIHVYTGLPWWGTIVTTTVIVRLLMFPLYVKSSDTIARNSKIKPELDAINSELMGTTDMVEAQRVALRRKKLMAENGIKNRYLAAPMLQVPVALGFFSGIRQMANYPVDGFTNQGLAWFQDLSLADPYLGLQVITASVFISFTRLGGETGAQQFSPAMKNFFTVMPLLSIPATMNLASGVVLYFAVNGACSVLQTLVLRNKWIRKKLDIADVVKRPASTTGPAKGIIETFKENMNKAREQAERRQLMQDNEKKLQEAAKEQKSNSAIKIVRKSDLKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2W4</accession>
    <name>C5E2W4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 10">Man(5)GlcNAc(2)-PP-dolichol translocation protein RFT1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0H08228g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Intramembrane glycolipid transporter that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. RFT1 could mediate the translocation of the cytosolically oriented intermediate DolPP-GlcNAc2Man5, produced by ALG11, into the ER lumen where dolichol-linked oligosaccharides assembly continues. However, the intramembrane lipid transporter activity could not be confirmed in vitro.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Endoplasmic reticulum membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the RFT1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556237.1">
        <property type="nucleotide sequence ID" value="XM_002556191.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2W4"/>
    <dbReference type="FunCoup" id="C5E2W4">
        <property type="interactions" value="731"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2W4"/>
    <dbReference type="GeneID" id="8294555"/>
    <dbReference type="KEGG" id="lth:KLTH0H08228g"/>
    <dbReference type="eggNOG" id="KOG2864">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023360_3_0_1"/>
    <dbReference type="InParanoid" id="C5E2W4"/>
    <dbReference type="OMA" id="WPGKLFG"/>
    <dbReference type="OrthoDB" id="9979195at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034203">
        <property type="term" value="P:glycolipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007594">
        <property type="entry name" value="RFT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13117">
        <property type="entry name" value="ENDOPLASMIC RETICULUM MULTISPAN TRANSMEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13117:SF5">
        <property type="entry name" value="PROTEIN RFT1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04506">
        <property type="entry name" value="Rft-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="141"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="178"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="217"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="361"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="398"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="430"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="461"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="503"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="527"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR30375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="567" mass="64006" checksum="55A98692C9358338" modified="2009-07-28" version="1">MEKRGQVSQHESMLKRSAKGVTFLMFSQLFSKIVTFILNTLLVRYLSPRVFGINAFLEFLLSTVLFFSREAIRMSTLRIKSPVAASSATEDDPETDDAGPGVLQTAINFAYIPLCIGVPLSLVLIGWQYSNLNEYFLSLPYFKLSIAMIWLSIIFELLNEPFFVVNQLMLNYKVRSQLESIAVVVSCIANFSIVYVYENKFNGSGIALHDATKQEGIAILAFAVSKLVHSTALLCCYYYDYLTRLAPAKKFNISPSKVQLSAGTQPYYLQSEVLQHFKKVYLQLCFKHLLTEGDKLIINSMCSVEEQGIYSLLSNYGSLLTRLLFAPIEESLRLFLARLLSVTSTKNTKLSMDVLVNLTKFYLYLSLLILVFGPTNSPFLLQFLIGSKWSSTSVLDTIQVYCLYLPFLSINGIFEAFFQSVASGEDILRQSYFMIASSGVFLLSCWLLISQYKMSLEGLIFSNIINMLLRIGFCGRFINAFYKRVYSDPASHDSTFLLNFSNLKAVSLVALVIALSNYFLFGVVKTFGQLSINAIMAFGLLSLILYKEKDIIRSYLKQSKRDEVKDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2Y4</accession>
    <name>C5E2Y4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">KLTH0H08800p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0H08800g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556257.1">
        <property type="nucleotide sequence ID" value="XM_002556211.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2Y4"/>
    <dbReference type="GeneID" id="8294581"/>
    <dbReference type="KEGG" id="lth:KLTH0H08800g"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1586773_0_0_1"/>
    <dbReference type="InParanoid" id="C5E2Y4"/>
    <dbReference type="OMA" id="ATLHECH"/>
    <dbReference type="OrthoDB" id="6077919at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001732">
        <property type="entry name" value="Zgc:162936"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000761">
        <property type="entry name" value="Zinc finger protein 449"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR PROTEIN YY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003:SF19">
        <property type="entry name" value="YY2 TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="111"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="139"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR30395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="168" mass="18419" checksum="7C08A01FAED423C5" modified="2009-07-28" version="1">MGLIEERGRDASQERGRGESQERGGRSLSEILREVKKPEIRSRARRGEGDAARDPHVPPPLTPTPPLVHTPPMVHTPPLPQSALPFAHMAPLAHPAALAVPARAPPSATLHECHVCGKTFSRPSGLNTHALIHTGHQPFVCDVPHCGKRFNVKSNLIRHKKIHDKPRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5E324</accession>
    <name>C5E324_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Actin-related protein 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12 14">Actin-like protein ARP4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">KLTH0H09702g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Chromatin interaction component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of selected genes principally by acetylation of nucleosomal histone H4 and H2A. The NuA4 complex is also involved in DNA repair. Is required for NuA4 complex integrity. Component of the SWR1 complex which mediates the ATP-dependent exchange of histone H2A for the H2A variant HZT1 leading to transcriptional regulation of selected genes by chromatin remodeling. Component of the INO80 complex which remodels chromatin by shifting nucleosomes and is involved in DNA repair.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the NuA4 histone acetyltransferase complex, of the INO80 chromatin remodeling complex, and of the SWR1 chromatin remodeling complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the actin family. ARP4 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556297.1">
        <property type="nucleotide sequence ID" value="XM_002556251.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E324"/>
    <dbReference type="FunCoup" id="C5E324">
        <property type="interactions" value="415"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E324"/>
    <dbReference type="GeneID" id="8294622"/>
    <dbReference type="KEGG" id="lth:KLTH0H09702g"/>
    <dbReference type="eggNOG" id="KOG0679">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027965_6_2_1"/>
    <dbReference type="InParanoid" id="C5E324"/>
    <dbReference type="OMA" id="MWLSRQE"/>
    <dbReference type="OrthoDB" id="5132116at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000812">
        <property type="term" value="C:Swr1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd13395">
        <property type="entry name" value="ASKHA_NBD_Arp4_ACTL6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000203">
        <property type="entry name" value="Actin-related protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000289">
        <property type="entry name" value="Actin-related protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000058">
        <property type="entry name" value="Putative actin-related protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="322"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="359"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00042445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00053941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00077253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAR30435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="481" mass="53503" checksum="467BCD6FDA5CBE66" modified="2009-07-28" version="1">MSNPALQIYGGDEISAVVIDPGSYSTNIGYSGTDSPQLNIPSCYGQYTAGGKEGQKSFNDQFMIMPRPDFEIKRILENGCVVDWEAAQEQWAWAIRDELKFESFKGTPAFLTEAIWNPEENRKKSLEVLLEGMEFEACYIAPTATCTSFAMGRPTCLVLDIGHDVASVCPVVDGMTLSKSSTRNFLAGKLLNKLIENHLKPRELVPLYKVERKRPEFVPKKFEFTVSKSVDEFANSRGFLQECKETMLHVAAVELPKFQSELETTSKRSIEAPWGENIIFDSNARYSFAEQLFKPTKEMLPSDWPVTKDGVVETWHNDYVPMKRAKPGLGNKDKEGTTESTPAPEEANGETPSATTNENGKRPIEESSKTSDAIAGLADLVNTAITATDVDLRATLAHNLVVTGGSSSIPGLTDRLMTELNKKLPALKFRILTSGHLRERQSRAWLGGSILSSLGTFHQLWVGRQEYEEVGAERLLKDRFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>C5E349</accession>
    <name>C5E349_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA mismatch repair protein HSM3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0H10384g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in DNA mismatch repair in slow-growing cells. Acts as a chaperone during the assembly of the 26S proteasome, specifically of the base subcomplex of the 19S regulatory complex (RC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the proteasome subunit S5B/HSM3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556322.1">
        <property type="nucleotide sequence ID" value="XM_002556276.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E349"/>
    <dbReference type="FunCoup" id="C5E349">
        <property type="interactions" value="139"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E349"/>
    <dbReference type="GeneID" id="8294653"/>
    <dbReference type="KEGG" id="lth:KLTH0H10384g"/>
    <dbReference type="eggNOG" id="ENOG502QWEK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044760_0_0_1"/>
    <dbReference type="InParanoid" id="C5E349"/>
    <dbReference type="OMA" id="YMEQMVL"/>
    <dbReference type="OrthoDB" id="4074002at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12794">
        <property type="entry name" value="Hsm3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040752">
        <property type="entry name" value="HSM3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041335">
        <property type="entry name" value="HSM3_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18794">
        <property type="entry name" value="HSM3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18795">
        <property type="entry name" value="HSM3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair protein HSM3 N-terminal" evidence="10">
        <location>
            <begin position="12"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="DNA mismatch repair protein HSM3 C-terminal" evidence="9">
        <location>
            <begin position="307"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF18794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF18795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR30460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="480" mass="55039" checksum="28CC29B58713E824" modified="2009-07-28" version="1">MAELTNNLSSDCLLDAITTSFNTKDYVTLNKQLNRANLQLCNATTLPGNKTEFLKSLKEIFSQAPEELDHSTTAEVFERVIGLTSFDQVLEAFTVEEMGHALSSHVINLVRLACKVLSRSQPKGLFAGTGLIDLMLEQLFDASTELDIVSEIEKCLEQLTSDALIRRRILKDNLQLLLRVRSQFDPICTTRLLALMNGILPHTDRSEFQDKLFIFGETEIHKALDKDIFLFINVTDYYVSILKIMGATSEQRQFSIWVLDRVLSVVIPIYGKLYKQMHEDYMIETFGRKYLFSLFKEISMLSDSHYFEELDSRYLNLTPSNLDFSEFMKFIKPSYLMEVQHDYILGNLTVTPSNLMAWRNLISEKQSFDQVKDNLTSDAILSLPYSEQMVLLQKLTCYEYSARYLLNDLSKVMSNTLDDKSGSITEPETSELRREVLENLLTFDSGMLNVWFQPTQEAYRLLVNGGKVQDSMAHVADTYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3B9</accession>
    <name>C5E3B9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">H/ACA ribonucleoprotein complex subunit CBF5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">H/ACA ribonucleoprotein complex subunit cbf5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0H12078g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a uridine in mRNA = a pseudouridine in mRNA</text>
            <dbReference type="Rhea" id="RHEA:56644"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:14659"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>uridine in 5S rRNA = pseudouridine in 5S rRNA</text>
            <dbReference type="Rhea" id="RHEA:47036"/>
            <dbReference type="Rhea" id="RHEA-COMP:11730"/>
            <dbReference type="Rhea" id="RHEA-COMP:11731"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>uridine in snRNA = pseudouridine in snRNA</text>
            <dbReference type="Rhea" id="RHEA:51124"/>
            <dbReference type="Rhea" id="RHEA-COMP:12891"/>
            <dbReference type="Rhea" id="RHEA-COMP:12892"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the pseudouridine synthase TruB family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556392.1">
        <property type="nucleotide sequence ID" value="XM_002556346.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3B9"/>
    <dbReference type="FunCoup" id="C5E3B9">
        <property type="interactions" value="1713"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3B9"/>
    <dbReference type="GeneID" id="8294729"/>
    <dbReference type="KEGG" id="lth:KLTH0H12078g"/>
    <dbReference type="eggNOG" id="KOG2529">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032087_3_2_1"/>
    <dbReference type="InParanoid" id="C5E3B9"/>
    <dbReference type="OMA" id="KYGRTNE"/>
    <dbReference type="OrthoDB" id="10250002at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031429">
        <property type="term" value="C:box H/ACA snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000495">
        <property type="term" value="P:box H/ACA sno(s)RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990481">
        <property type="term" value="P:mRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031118">
        <property type="term" value="P:rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031120">
        <property type="term" value="P:snRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02572">
        <property type="entry name" value="PseudoU_synth_hDyskerin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21148">
        <property type="entry name" value="PUA_Cbf5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2350.10:FF:000001">
        <property type="entry name" value="H/ACA ribonucleoprotein complex subunit CBF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.10">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="entry name" value="PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012960">
        <property type="entry name" value="Dyskerin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002501">
        <property type="entry name" value="PsdUridine_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004802">
        <property type="entry name" value="tRNA_PsdUridine_synth_B_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032819">
        <property type="entry name" value="TruB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004521">
        <property type="entry name" value="Uncharacterised_CHP00451"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00425">
        <property type="entry name" value="CBF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003280">
        <property type="entry name" value="PRK04270.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00451">
        <property type="entry name" value="unchar_dom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23127">
        <property type="entry name" value="CENTROMERE/MICROTUBULE BINDING PROTEIN CBF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23127:SF0">
        <property type="entry name" value="H_ACA RIBONUCLEOPROTEIN COMPLEX SUBUNIT DKC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08068">
        <property type="entry name" value="DKCLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16198">
        <property type="entry name" value="TruB_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01509">
        <property type="entry name" value="TruB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01136">
        <property type="entry name" value="DKCLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dyskerin-like" evidence="10">
        <location>
            <begin position="17"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="PUA" evidence="9">
        <location>
            <begin position="266"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="379"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="379"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="394"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="417"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="444"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="467"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00072225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR30530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="484" mass="54478" checksum="C66575DAFA4C2F7A" modified="2009-07-28" version="1">MSEEYLIKPENVAPTTDASEWPLLLKNYDKLLVRSGHYTPIPTGSSPHKRDLKSYISSGVINLDKPSNPSSHEVVAWIKRILRCEKTGHSGTLDPKVTGCLIVCVDRATRLVKSQQGAGKEYVCIVRLHDALKDEKDLGRGLENLTGALFQRPPLISAVKRQLRVRTIYDSNLIEFDTNRNLGVFWASCEAGTYMRTLCVHLGMLLGVGGHMQELRRVRSGALSENDNLVTLHDVMDAQWVYDNTRDESYLRKIIQPLETLLLGYKRIVVKDSAVNAVCYGAKLMIPGLLRYEEGIELYDEVVLMTTKGEAIAIAIAQMSTVDLATCDHGVVAKVKRCIMERDLYPRRWGLGPVAQKKKQMKADGKLDKYGRVNEQTPEDWKKSYVSHEGDQPAAQVSSTVTESVSATSDSAPVQEMVKEVKVETIVKESEDDEDKEESGKKEKKDKKDKKEKKEKKEKKEKKDKKRKAEGEESADKKKKKSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3K4</accession>
    <name>C5E3K4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">KLTH0H14300g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="3"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556477.1">
        <property type="nucleotide sequence ID" value="XM_002556431.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3K4"/>
    <dbReference type="FunCoup" id="C5E3K4">
        <property type="interactions" value="706"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3K4"/>
    <dbReference type="GeneID" id="8294839"/>
    <dbReference type="KEGG" id="lth:KLTH0H14300g"/>
    <dbReference type="eggNOG" id="KOG0387">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000315_7_0_1"/>
    <dbReference type="InParanoid" id="C5E3K4"/>
    <dbReference type="OMA" id="PTWTGQF"/>
    <dbReference type="OrthoDB" id="413460at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006283">
        <property type="term" value="P:transcription-coupled nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22254">
        <property type="entry name" value="CSB_WHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18000">
        <property type="entry name" value="DEXHc_ERCC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000039">
        <property type="entry name" value="DNA repair protein Rhp26/Rad26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050496">
        <property type="entry name" value="SNF2_RAD54_helicase_repair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058951">
        <property type="entry name" value="WHD_Rad26_CSB-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45629:SF7">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45629">
        <property type="entry name" value="SNF2/RAD54 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25875">
        <property type="entry name" value="WHD_Rad26_CSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="281"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="15">
        <location>
            <begin position="625"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="104"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="143"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="228"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="109"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="228"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAR30615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1037" mass="117734" checksum="EC807A31955CFD56" modified="2009-07-28" version="1">MDGSEAQLEQLGIELVSQSALENEIESNAQSLIDKRSLEQEHQRLERSQNALGKLLTKREMLKRKMRAASRISVRAKLEAQIRELDENEIRSLEDDVSDIRSRLKELSKSTADNNETAPSERQPGESERDYLIRTGKTTAFGTESGFILDRDGDEDPTVLSDGHESVTSDNEEDYQVEADKSDASSEGLASEFSESAEDKEAPKAASFIDDGDELNYQKRLKNWVRERSSRRLKDPNPELEEYRKPNPGLPDATLNDDFRIPGEIFQSLFNYQKTCVQWLYELYQQQCGGIIGDEMGLGKTIQIIAFLASLHHSGKLNGPILVVCPATVLRQWCKEFHTWWPPFRAIILHSIGAGMTQKENLSEQKLEELFMNSNPEEFSFDAYTNSKRTKSILESSLTRDNLINKVVTDGHVLITTYVGLRIHSDKLLNVRWGYAVLDEGHKIRNPDADISLTCKQLKTKNRIILSGTPIQNNLTELWSLFDFIFPGRLGTLPVFQQQFSVPINMGGYANATNIQVQTGYKCAVALRNLISPYLLRRIKADVAKDLPRKNEMVLFCKLTQYQRNKYLQFLNSEDLVKIKNGKRQVLFGIDILRKICNHPDLLEREQRQNEDSYGDPKRSGKMQVVKQLLKLWHSQGHKTLLFTQSRQMLDILERFISYKDPELEELTYLRMDGTTSIGARQALVDSFNNGSYDVFLLTTRVGGLGVNLTGANRIIIFDPDWNPSTDMQARERAWRIGQRREVTIYRLMIAGSIEEKIYHRQIFKQFLTNKILTDPKQKRFFKMNELQDLFTLGGETGLTNENLESEVQKQTENIKKSRSDESDDFDKVVQLSGVSKLEGFFGGKENEEKNKGEEERLMDGLFGGNVEGVKSHSSVIEQHSKPSTDIITREATKVAEEALAALKKSRKTTKKFEIGTPTWTGKFGAAGRVTKKKSATSLSGSAAILSNLRKGQIKNGSDTTNEPSKEIKKGSELLGKIQEYLKRKGGYSAKSAEILEHTGFRLNEKEDITKVRALLKQIATFESGKGRWLLKDEFRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3L7</accession>
    <name>C5E3L7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0H14608p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0H14608g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556490.1">
        <property type="nucleotide sequence ID" value="XM_002556444.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3L7"/>
    <dbReference type="FunCoup" id="C5E3L7">
        <property type="interactions" value="58"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3L7"/>
    <dbReference type="GeneID" id="8294853"/>
    <dbReference type="KEGG" id="lth:KLTH0H14608g"/>
    <dbReference type="eggNOG" id="ENOG502S5QU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028690_2_0_1"/>
    <dbReference type="InParanoid" id="C5E3L7"/>
    <dbReference type="OMA" id="MWAGSQF"/>
    <dbReference type="OrthoDB" id="3257538at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019171">
        <property type="term" value="F:(3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052741">
        <property type="entry name" value="Mitochondrial_HTD2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28152">
        <property type="entry name" value="HYDROXYACYL-THIOESTER DEHYDRATASE TYPE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28152:SF1">
        <property type="entry name" value="HYDROXYACYL-THIOESTER DEHYDRATASE TYPE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR30628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="280" mass="31630" checksum="A01DAB02ED005631" modified="2009-07-28" version="1">MNGAHSALKWGFKELVSDAPLTRFQSMVSERMPLKLTQGHTGLQTLSPGDHLLYFNPQRETLDVDGYFKYQTPAALLHRPLAFKRRMWALGAMSFKNPLRIGHSYECIETIKFVKKIRQDYYVGINRQIFEPSSNVSFVDEFRTLVYTDSAPAPISPAAAHMQNPVSSVRLTFYESDVFRYSSLTFNPHRVHWDHKYCTEVEGYSSPIVQGPFIVHALLKYAQCLGVRVGAVKYKNSQYLSSGSTVEMCIAAPVEGRCEAHVRDPQAPNIIFCKASLTLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3N4</accession>
    <name>C5E3N4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glutamine-dependent NAD(+) synthetase</fullName>
            <ecNumber evidence="8">6.3.5.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">NAD(+) synthase [glutamine-hydrolyzing]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0H15004g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>deamido-NAD(+) + L-glutamine + ATP + H2O = L-glutamate + AMP + diphosphate + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24384"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (L-Gln route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">In the C-terminal section; belongs to the NAD synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.1" evidence="8"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556507.1">
        <property type="nucleotide sequence ID" value="XM_002556461.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3N4"/>
    <dbReference type="FunCoup" id="C5E3N4">
        <property type="interactions" value="786"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3N4"/>
    <dbReference type="GeneID" id="8294871"/>
    <dbReference type="KEGG" id="lth:KLTH0H15004g"/>
    <dbReference type="eggNOG" id="KOG2303">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011884_2_0_1"/>
    <dbReference type="InParanoid" id="C5E3N4"/>
    <dbReference type="OMA" id="TSQEVCN"/>
    <dbReference type="OrthoDB" id="2020662at2759"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00334"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003952">
        <property type="term" value="F:NAD+ synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07570">
        <property type="entry name" value="GAT_Gln-NAD-synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00553">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000036">
        <property type="entry name" value="Glutamine-dependent NAD(+) synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.110.10:FF:000003">
        <property type="entry name" value="Glutamine-dependent NAD(+) synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02090">
        <property type="entry name" value="NadE_glutamine_dep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014445">
        <property type="entry name" value="Gln-dep_NAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022310">
        <property type="entry name" value="NAD/GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003694">
        <property type="entry name" value="NAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00552">
        <property type="entry name" value="nadE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23090:SF9">
        <property type="entry name" value="GLUTAMINE-DEPENDENT NAD(+) SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23090">
        <property type="entry name" value="NH 3 /GLUTAMINE-DEPENDENT NAD + SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02540">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006630">
        <property type="entry name" value="NADS_GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 8" id="KW-0520">NAD</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="9">
        <location>
            <begin position="5"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00052340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR006630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR30645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="714" mass="80923" checksum="AA50B9FA6F189420" modified="2009-07-28" version="1">MSHLVTVATCNLNQWALDFEGNRDRILESIRIAKERGARLRVGPELEVSGYGCLDHFLENDLYLHCWEMYAQIIKREDTRGILLDIGMPVLHKNVRYNCRLLSFDGRILFIRPKIWLANDGNYREMRFFTPWMKATQVEEFLLPPLIQKITGQRIVPFGDAVIRTLDTCIGAETCEELFTPQSPHIAMSLDGVEIITNSSGSHHELRKLNRRLELITSATRRCGGVYLYANQRGCDGDRLYYDGCALIAVNGKIVAQGSQFSLKDVEVVTAAVDLEEVRNHRASIISRGLQAAESKVVFQRIDLEEELAPMGNRFNPKISPAKAREFHYHTPEEEIALGPACWLWDYLRRCNGTGFFLPLSGGIDSCATAVIVHSMCRLVVQECKEGNEQVLADARKLARKDPEWVPATPQDLASCLFHTCFMGTTNSSKDTRSRARELAKVISSYHVDFNMDNVVSSVVSLFEITTGKKPIYKIFGGSQVENLALQNIQARLRMVLAYLFAQLLPWVRRIPNGGSLLVLGSANVDECLRGYLTKYDCSSADINPIGGISKTDLKKFISYASKAFDMPILDEFVSATPTAELEPTTKDYVQSDEIDMGMTYEELSIFGYLRKVEKCGPYSMFLKLLHEWTPRLSPAQVAEKVKRFFFFYAINRHKQTVITPSYHAEQYSPDDNRFDLRPFLINPRFAWASKKIDQVVEQCEGKDACELDIMSVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3S1</accession>
    <name>C5E3S1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0H15862p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0H15862g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556544.1">
        <property type="nucleotide sequence ID" value="XM_002556498.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3S1"/>
    <dbReference type="FunCoup" id="C5E3S1">
        <property type="interactions" value="174"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3S1"/>
    <dbReference type="GeneID" id="8294910"/>
    <dbReference type="KEGG" id="lth:KLTH0H15862g"/>
    <dbReference type="eggNOG" id="KOG2447">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079423_0_0_1"/>
    <dbReference type="InParanoid" id="C5E3S1"/>
    <dbReference type="OMA" id="SIVTHYV"/>
    <dbReference type="OrthoDB" id="432292at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008250">
        <property type="term" value="C:oligosaccharyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056790">
        <property type="entry name" value="Ribophorin_II_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008814">
        <property type="entry name" value="Swp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12640:SF0">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12640">
        <property type="entry name" value="RIBOPHORIN II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25147">
        <property type="entry name" value="Ribophorin_II_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044205813" evidence="8">
        <location>
            <begin position="20"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="190"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="223"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="261"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="Ribophorin II C-terminal" evidence="9">
        <location>
            <begin position="180"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF25147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR30682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="281" mass="31224" checksum="463D1FBF47F99416" modified="2009-07-28" version="1">MLFKQIALAALLGVTSVLALSAKQPHVAFVTSKQQPVALRTISDQFQKFDKPLVVERQNDTIEFAFSIEREQKPQHASLALGLVERGIEAHYSPIIKDNEKEGMSTYKFRIPIERLSDSLLHYGAELHEPLTASLLLANKNGGSDNVFVELFDLSLAFEINRKLSLPARFQPLPEIKHIFNAPPTIVSSWFAQMFSFIILGCVLSLLLVWLKAGIFSSAKLPLNTVIAIYYLGFVGAIIGLEYVFTQYYLGASIFKTLENAFYVSAPGLLIGCKLLVSLTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBA0</accession>
    <name>C5DBA0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0A00836p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0A00836g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551499.1">
        <property type="nucleotide sequence ID" value="XM_002551453.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBA0"/>
    <dbReference type="FunCoup" id="C5DBA0">
        <property type="interactions" value="1269"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBA0"/>
    <dbReference type="GeneID" id="8290290"/>
    <dbReference type="KEGG" id="lth:KLTH0A00836g"/>
    <dbReference type="eggNOG" id="KOG2014">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002556_4_1_1"/>
    <dbReference type="InParanoid" id="C5DBA0"/>
    <dbReference type="OMA" id="EFFGQFD"/>
    <dbReference type="OrthoDB" id="1708823at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031510">
        <property type="term" value="C:SUMO activating enzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019948">
        <property type="term" value="F:SUMO activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF162">
        <property type="entry name" value="SUMO-ACTIVATING ENZYME SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="THIF-type NAD/FAD binding fold" evidence="1">
        <location>
            <begin position="3"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="320" mass="35637" checksum="3CB28339C5B16F2B" modified="2009-07-28" version="1">MAAQARMRSAKVLLINLGGLGTEIAKNLVLSGVGFLSLVDNHDVSEGDLSTQFFLSKDEIGTKRLDSAISRIQDMNPRVTLTVDTEDFRQKPDSFYGQFDLIIATDVSTEEAIRVNQLTRKFNVPFFLCGLNGLSGFIFVDLIRFDATDEKLKSSVKTPLGALSQNREVVEVTEYLNEEQSEVYERIVTRNAYKPFEQVLKEGTLKGKLTKRQVKRLTNAVPLTFALFSYRDGYAAVTRHDLEQRATDACVQLGLPSENLKQEYVEQFSQQAGLEFAPVSAVIGGTVAQDVINILGKKQSPLNNFIVLDGISLDMHIFEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBA7</accession>
    <name>C5DBA7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0A00990p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0A00990g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551506.1">
        <property type="nucleotide sequence ID" value="XM_002551460.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBA7"/>
    <dbReference type="FunCoup" id="C5DBA7">
        <property type="interactions" value="42"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBA7"/>
    <dbReference type="GeneID" id="8290297"/>
    <dbReference type="KEGG" id="lth:KLTH0A00990g"/>
    <dbReference type="eggNOG" id="ENOG502S00X">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078856_1_0_1"/>
    <dbReference type="InParanoid" id="C5DBA7"/>
    <dbReference type="OMA" id="HPTIIMT"/>
    <dbReference type="OrthoDB" id="5300823at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052841">
        <property type="entry name" value="PMP_oxidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011576">
        <property type="entry name" value="Pyridox_Oxase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28040">
        <property type="entry name" value="PYRIDOXAMINE 5'-PHOSPHATE OXIDASE YLR456W HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28040:SF1">
        <property type="entry name" value="PYRIDOXAMINE 5'-PHOSPHATE OXIDASE YLR456W HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01243">
        <property type="entry name" value="PNPOx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyridoxamine 5'-phosphate oxidase N-terminal" evidence="2">
        <location>
            <begin position="7"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR21064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="210" mass="23348" checksum="0476F62328FEDA96" modified="2009-07-28" version="1">MSCPDSLPEHVLSLLKTSKYLHLATCSKPECIPSVSLMNYTYISPEKVFVADKEKGATGERRHYIIMATQRDTEKYENIAQNPEVSVLIHDWVTAKKLSLRKSSMSNTPTPEPQSAAAAAAAQNPDHPSRLLNLLQELNQSELSQMSATLRGRAEAVDPSSEEFRYYEEALLKANPDAGVYTKGQNVAVVKVRLESAKVSDRENNTSFYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBC3</accession>
    <name>C5DBC3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0A01364p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0A01364g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551522.1">
        <property type="nucleotide sequence ID" value="XM_002551476.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBC3"/>
    <dbReference type="FunCoup" id="C5DBC3">
        <property type="interactions" value="32"/>
    </dbReference>
    <dbReference type="GeneID" id="8290314"/>
    <dbReference type="KEGG" id="lth:KLTH0A01364g"/>
    <dbReference type="eggNOG" id="ENOG502R6CM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_555750_0_0_1"/>
    <dbReference type="InParanoid" id="C5DBC3"/>
    <dbReference type="OMA" id="PVIPWDA"/>
    <dbReference type="OrthoDB" id="4070119at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014804">
        <property type="entry name" value="Pet20-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08692">
        <property type="entry name" value="Pet20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="39"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="51"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR21080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="476" mass="54740" checksum="F852BB8EA39F0E99" modified="2009-07-28" version="1">MFRNLAHLYNGKFQTLNRTNALWERCSCMRLCSTDANAAEKSKEEEEEVEVATKENGKKDGGDWNGSNAYSCGRPSRKSGKGVYLVPKVHSTDHIKQDEIHTEGLFAGNKPLFLGELSLSFKRPSGALDGIFGTLTKIKRATDDGGDQEIDVQGIINDLQTGDKDISSGEKSAAKRPVIPWDASISGMVYNDQPFKQVPRGVVNKLKPFKLLKIERKNKSGNSQDHELVKMRVHNSKVNDELQLVDLNREATRRPPTLELNDSAGKAKRFYTDESKSLTQFKFIRSDQHVFKTDVDKLNNLLVKEFLKRTKLKIDSEFRDNQLPLYIYINKTLSSNALLQRFIRKRVTDHISPLFTTILSSYEREDEAKKFEKRIQLRVNSLVEDLAEYFPSVFFAGDIVDCVHHPSPVPGYGRLYWLKPGKRHKAIWGRNSESDFVFNLSNTYRITRSGVRYMKYPINIHYRTFHQAFTEWEYRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBD7</accession>
    <name>C5DBD7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0A01716p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0A01716g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551536.1">
        <property type="nucleotide sequence ID" value="XM_002551490.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBD7"/>
    <dbReference type="FunCoup" id="C5DBD7">
        <property type="interactions" value="131"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBD7"/>
    <dbReference type="GeneID" id="8290329"/>
    <dbReference type="KEGG" id="lth:KLTH0A01716g"/>
    <dbReference type="eggNOG" id="KOG1548">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026945_2_0_1"/>
    <dbReference type="InParanoid" id="C5DBD7"/>
    <dbReference type="OMA" id="TRYNGEH"/>
    <dbReference type="OrthoDB" id="10258585at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005684">
        <property type="term" value="C:U2-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12285">
        <property type="entry name" value="RRM3_RBM39_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034393">
        <property type="entry name" value="TatSF1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15608:SF0">
        <property type="entry name" value="HIV TAT-SPECIFIC FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15608">
        <property type="entry name" value="SPLICING FACTOR U2AF-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="43"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="127"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="127"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="161"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="177"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="187"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR21094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="296" mass="33760" checksum="5FDB524AFF99C2B6" modified="2009-07-28" version="1">MDEEELQLKKELREKKAQELARRKRARDNTERKEDTKRPLKNCAIYISHLPLEVTKDEVIEEFTKYGVIRKDLKSSEPKCKFYYGVDGSFEGAALIVYMRPESVRMAVDLMDGYSFMGNKLKVEEATFKKEPKDDKKSPNASQEGSRDVSAEPKLNNIHAKLNEQERELQDWDDTTDEKTTPREEGSEVDSIPDESENASSRTVVLANVLDLYANLAPQQIAEVAADLKEGCEAIGSVSSFEFDEVLGQAKVEYKSSEIAQKCCQLMNGRYFDGRKLVAYNLEQGEEEEEDEVAEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBN0</accession>
    <name>C5DBN0_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">NADH-cytochrome b5 reductase</fullName>
            <ecNumber evidence="17">1.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ordered locus">KLTH0A03982g</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>2 Fe(3+)-[Dph3] + NADH = 2 Fe(2+)-[Dph3] + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:71231"/>
            <dbReference type="Rhea" id="RHEA-COMP:18002"/>
            <dbReference type="Rhea" id="RHEA-COMP:18003"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:83228"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:71232"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 17">
            <text>2 Fe(III)-[cytochrome b5] + NADH = 2 Fe(II)-[cytochrome b5] + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46680"/>
            <dbReference type="Rhea" id="RHEA-COMP:10438"/>
            <dbReference type="Rhea" id="RHEA-COMP:10439"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 16 17">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion outer membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.6.2.2" evidence="17"/>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551629.1">
        <property type="nucleotide sequence ID" value="XM_002551583.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBN0"/>
    <dbReference type="FunCoup" id="C5DBN0">
        <property type="interactions" value="278"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBN0"/>
    <dbReference type="GeneID" id="8290432"/>
    <dbReference type="KEGG" id="lth:KLTH0A03982g"/>
    <dbReference type="eggNOG" id="KOG0534">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003827_9_0_1"/>
    <dbReference type="InParanoid" id="C5DBN0"/>
    <dbReference type="OMA" id="LDMKGPF"/>
    <dbReference type="OrthoDB" id="432685at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090524">
        <property type="term" value="F:cytochrome-b5 reductase activity, acting on NADH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd06183">
        <property type="entry name" value="cyt_b5_reduct_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000032">
        <property type="entry name" value="NADH-cytochrome b5 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.80:FF:000009">
        <property type="entry name" value="NADH-cytochrome b5 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001834">
        <property type="entry name" value="CBR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008333">
        <property type="entry name" value="Cbr1-like_FAD-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19370">
        <property type="entry name" value="NADH-CYTOCHROME B5 REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19370:SF184">
        <property type="entry name" value="NADH-CYTOCHROME B5 REDUCTASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00970">
        <property type="entry name" value="FAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00406">
        <property type="entry name" value="CYTB5RDTASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16 17" id="KW-0274">FAD</keyword>
    <keyword evidence="5 16 17" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="13 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="11 17" id="KW-0520">NAD</keyword>
    <keyword evidence="10 17" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="19">
        <location>
            <begin position="41"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR601834-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU361226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CAR21187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="287" mass="31284" checksum="6D6E75149AF3F869" modified="2009-07-28" version="1">MDSLPFDSKTLLIGGFVAFFVITLASRFLGSKKAIPVLIKDQYQEFPLVSKTVLTHNTAVYRFGLPKQSDVLGLPIGQHISIKGNLPDGKEIMRSYTPTSLDSDSVGFFELLIKSYDQGNVSKMIGELQIGDKIKVRGPKGFYTYTPNMNSEIGMIAGGTGIAPMYQIIKAIHSDPKDRTKVSIVYGSQTEDDILLKKELDAIVESNPEQFKVHYLLDKPARDTWTGGVGYVTPDVMKSYLPAPGPGAQLLVCGPPGMVSSVKKNAVALGFEKAKPVSKMGDQVFVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DC43</accession>
    <name>C5DC43_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">poly(A)-specific ribonuclease</fullName>
            <ecNumber evidence="5">3.1.13.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0A07656g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Exonucleolytic cleavage of poly(A) to 5'-AMP.</text>
            <dbReference type="EC" id="3.1.13.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CAF1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.13.4" evidence="5"/>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551792.1">
        <property type="nucleotide sequence ID" value="XM_002551746.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DC43"/>
    <dbReference type="FunCoup" id="C5DC43">
        <property type="interactions" value="1042"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DC43"/>
    <dbReference type="GeneID" id="8290599"/>
    <dbReference type="KEGG" id="lth:KLTH0A07656g"/>
    <dbReference type="eggNOG" id="KOG0304">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027974_3_1_1"/>
    <dbReference type="InParanoid" id="C5DC43"/>
    <dbReference type="OMA" id="WQFNFLY"/>
    <dbReference type="OrthoDB" id="1164111at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030014">
        <property type="term" value="C:CCR4-NOT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004535">
        <property type="term" value="F:poly(A)-specific ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000107">
        <property type="entry name" value="Poly(A) ribonuclease POP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039637">
        <property type="entry name" value="CNOT7/CNOT8/Pop2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006941">
        <property type="entry name" value="RNase_CAF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10797">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04857">
        <property type="entry name" value="CAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="13" id="KW-0804">Transcription</keyword>
    <keyword evidence="12" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR21350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="422" mass="47503" checksum="1F83CE39627C41CA" modified="2009-07-28" version="1">MQSVNRQIHELPIGEQFFPQQTQQHQHPPGIGPQIFSPQLLQARLQQQQGGLVQDVHGIEKNQNLQNVYLLKQKLEAANAAFGQQQVPQRQSQTQQMLNNGSQNATSAALGSLAGLPPGVGMVQHGTSGMGPPSGAIHPQLAMGSFSLPPPTYFVVREVWSNNLHAEFMSIRKLVDQYNYVSISTEFVGTIARPMGNFRSKNDYHYQTMRANVDLLNPVQIGISLSDANGNKPENKHSTWQFNFHFDVTKEMVSAESLELLKKSGINFERHQNFGVLAFEFAQLLIDSGLISDNVTWVSYHAAYDFGFLVNMLMNNSMPNNKEDYVWWVQQFVPNFYDLNLINKFSQQHPSQLQQQHQPQFTLETMADELGIPRFSLFSSTAGQSLLALLTFTTLLNLPLPQPQLGPDLTRYKNMIYGITNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DC58</accession>
    <name>C5DC58_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0B00176p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0B00176g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551807.1">
        <property type="nucleotide sequence ID" value="XM_002551761.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DC58"/>
    <dbReference type="FunCoup" id="C5DC58">
        <property type="interactions" value="89"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DC58"/>
    <dbReference type="GeneID" id="8290624"/>
    <dbReference type="KEGG" id="lth:KLTH0B00176g"/>
    <dbReference type="eggNOG" id="KOG0634">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_0_6_1"/>
    <dbReference type="InParanoid" id="C5DC58"/>
    <dbReference type="OMA" id="DFLQWPV"/>
    <dbReference type="OrthoDB" id="7042322at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047536">
        <property type="term" value="F:2-aminoadipate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000080">
        <property type="entry name" value="Aminotransferase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42858">
        <property type="entry name" value="AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42858:SF1">
        <property type="entry name" value="LD15494P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="1">
        <location>
            <begin position="40"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="448" mass="49443" checksum="9A19063E9A066212" modified="2009-07-28" version="1">MKSIINLFKGYASHDLLPRKDIIEATSEVLQPEVREYDYNDEDRHPLVYGSDNGSAWVRACIAEFSNKVFKLPVHSSAATRPDHLNLTSGASYGIMNILVQTTLPHTGYTKQAFIVTPTYFLVNKAFLDAGFTGKITAVEEKGDGLDLAFLEAKLKLFENSANGEREQKTEESLDIINAQGGQKKVYRYVIYLVPTYSNPTGAIYSRSSRLKLLELARKYDILIISDDVYDLLRLDDDNGSGALIPSPPELRFPHLDRESISAANTFGNTISNCTFSKIIAPGLRTGYQETANANLATQLASGGANVSGGTPCQLNSMIVATMIKNGAVERIVHNFVSVLRERAMVLAESIKLYLPKGTQYSECKGGYFSWCTLPVGYDSSKIVEEVSHEGVLLADGSHFEVSGDKRNWGERSVRLSISYVTAAEIQEAVKTWGQFCEKHRYLSSKGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCD3</accession>
    <name>C5DCD3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA-directed RNA polymerase III subunit RPC9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0B02134g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic RPC9 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551882.1">
        <property type="nucleotide sequence ID" value="XM_002551836.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCD3"/>
    <dbReference type="FunCoup" id="C5DCD3">
        <property type="interactions" value="228"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCD3"/>
    <dbReference type="GeneID" id="8290712"/>
    <dbReference type="KEGG" id="lth:KLTH0B02134g"/>
    <dbReference type="eggNOG" id="KOG4168">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092529_3_0_1"/>
    <dbReference type="InParanoid" id="C5DCD3"/>
    <dbReference type="OMA" id="PTNMVHL"/>
    <dbReference type="OrthoDB" id="1746530at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006384">
        <property type="term" value="P:transcription initiation at RNA polymerase III promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010997">
        <property type="entry name" value="HRDC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005574">
        <property type="entry name" value="Rpb4/RPC9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038324">
        <property type="entry name" value="Rpb4/RPC9_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038846">
        <property type="entry name" value="RPC9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15561">
        <property type="entry name" value="CALCITONIN GENE-RELATED PEPTIDE-RECEPTOR COMPONENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15561:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03874">
        <property type="entry name" value="RNA_pol_Rpb4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47819">
        <property type="entry name" value="HRDC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR21444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="155" mass="17772" checksum="5D8A4276077DA87B" modified="2009-07-28" version="1">MKIESVRDAFLSDYEVLQFLSQLERRHDWGLNQDDELAAGNNRKRKRRPYNHPELQAITRDTIRYLSEAKGAADAEESEAGAGDTKSPLTKLNDKKFAELMTQLNQFSLFKAEKLQIVNQLPTNLVHLYAIVEECDSRFSEEQSGQILEIVRNAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCH0</accession>
    <name>C5DCH0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0B02992p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0B02992g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551919.1">
        <property type="nucleotide sequence ID" value="XM_002551873.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCH0"/>
    <dbReference type="STRING" id="559295.C5DCH0"/>
    <dbReference type="GeneID" id="8290751"/>
    <dbReference type="KEGG" id="lth:KLTH0B02992g"/>
    <dbReference type="eggNOG" id="KOG4209">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1343478_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCH0"/>
    <dbReference type="OMA" id="MNETIFR"/>
    <dbReference type="OrthoDB" id="4726at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF12">
        <property type="entry name" value="EUKARYOTIC INITIATION FACTOR 4B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="70"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="140"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="158"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR21481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="204" mass="23342" checksum="2D1B245B7F872B7A" modified="2009-07-28" version="1">MSDTRWTTSYPHMNEFLSDSSQQDNKPDYYHVITEETSRLKDIEKRMAENAHGAGCPGFSRFNDQSADARSVYVGNLDPSINAETLQEFFKGSVSRVNRVEIQINKLTGFAKGFAFVEFAQPRDVMDAIKLSNTKLGGKPLKIVPKRPSFHQESPNRGSHRMRRATTRTHPPLRGRPHGRHLHNAWRGNRRGRVRARGRGKRFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCI3</accession>
    <name>C5DCI3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0B03300p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0B03300g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SYS1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551932.1">
        <property type="nucleotide sequence ID" value="XM_002551886.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCI3"/>
    <dbReference type="FunCoup" id="C5DCI3">
        <property type="interactions" value="263"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCI3"/>
    <dbReference type="GeneID" id="8290765"/>
    <dbReference type="KEGG" id="lth:KLTH0B03300g"/>
    <dbReference type="eggNOG" id="KOG4697">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081382_1_0_1"/>
    <dbReference type="InParanoid" id="C5DCI3"/>
    <dbReference type="OMA" id="EYEMVGM"/>
    <dbReference type="OrthoDB" id="542931at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034067">
        <property type="term" value="P:protein localization to Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019185">
        <property type="entry name" value="Integral_membrane_SYS1-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12952:SF0">
        <property type="entry name" value="PROTEIN SYS1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12952">
        <property type="entry name" value="SYS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09801">
        <property type="entry name" value="SYS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="30"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="106"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="132"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR21494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="188" mass="21826" checksum="EBC1B4C2CCD73C8F" modified="2009-07-28" version="1">MVTIRRYLRIPQELRPTQIFRQDSLSPIKIALQIILLQLFYYCTAYILFYGWARLGGYDVSITKWLFTWEFIDFSNSLGIVLSLLWLLDSLICVLFLTVIVGRSKLAWDFGITIHAVNLLVVWINTGRWPSLSWFVVQLLSSAILVFLGTWTTRWSELRDTFFDGLVSSQPNPEGQVEMKDLEAQKPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCP1</accession>
    <name>C5DCP1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0B04664g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551990.1">
        <property type="nucleotide sequence ID" value="XM_002551944.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCP1"/>
    <dbReference type="FunCoup" id="C5DCP1">
        <property type="interactions" value="243"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCP1"/>
    <dbReference type="GeneID" id="8290827"/>
    <dbReference type="KEGG" id="lth:KLTH0B04664g"/>
    <dbReference type="eggNOG" id="KOG0922">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001832_5_11_1"/>
    <dbReference type="InParanoid" id="C5DCP1"/>
    <dbReference type="OMA" id="APVHDFV"/>
    <dbReference type="OrthoDB" id="10253254at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045943">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022613">
        <property type="term" value="P:ribonucleoprotein complex biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17917">
        <property type="entry name" value="DEXHc_RHA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000028">
        <property type="entry name" value="ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000615">
        <property type="entry name" value="pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000145">
        <property type="entry name" value="probable ATP-dependent RNA helicase DHX40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF118">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="73"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="255"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="24"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR21552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="700" mass="78022" checksum="3B5CCA31FFEE5D9E" modified="2009-07-28" version="1">MSKGKKFRKIGKTSFQPPKNVVFSDKRSSADGSSDHEDGLTKSASKHDLAREAASLLKVRATLPVYKHKDSIMSHINKNQVTILIGETGSGKSTQIPQFLMENFPEKTDKRIAVTQPRRVAAINLASRVAQEYGCRLGERVGYSVRFDNKSNARTKLKYLTDGMLLREIMLDKNLSDYSCVIIDEAHERTILTDLILGFLKELINSSRQDLKVVVMSATLQAEKFSEFFGRAPILFVEGRKFPVQRSYLKAQCDDVVDAIIRCCVQLNQSEQMGDILCFMPGQEEIDKAVAILSKVGEHLGASVPRLVALPLYAALPPAEQAKVFAPVKGFKRKVVFSTNLAETSVTISGVKFVVDSGLRKVKVWRHQLGLATLLTVPISKASASQRTGRAGRESAGKCFRLYREQDYQKLPNQTEPEIARSEVTSPILMLKKVGVKDIVNWSWLENPGKQAILMALQELFQLGALDDSGKITNKGQKMALLPLAPHLSSVLLKAHEDGCLLPVIDIVSCLSVENLLINPSPEQRDEVNEKRFSIATRGSKYGDLVMLKEFYDAYMSLKSAAEKDDWCKELCVSKRGFKNVVKIRAQLLSYMERLFKEKLSDNEDESDSFDIKAIVKCFLAGFAKNTAIGMPDRSFRTSTTGETISVHPSSMLFMDRSCPAILYTEYVFTTKGYARNVSRLELSWLQEVAGISTVKVSAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCW8</accession>
    <name>C5DCW8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0B06402p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0B06402g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552067.1">
        <property type="nucleotide sequence ID" value="XM_002552021.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCW8"/>
    <dbReference type="STRING" id="559295.C5DCW8"/>
    <dbReference type="GeneID" id="8290906"/>
    <dbReference type="KEGG" id="lth:KLTH0B06402g"/>
    <dbReference type="eggNOG" id="KOG1502">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_9_2_1"/>
    <dbReference type="InParanoid" id="C5DCW8"/>
    <dbReference type="OrthoDB" id="2735536at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF564">
        <property type="entry name" value="STEROL-4-ALPHA-CARBOXYLATE 3-DEHYDROGENASE, DECARBOXYLATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="3">
        <location>
            <begin position="5"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR21629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="361" mass="40783" checksum="5890BA6940E21931" modified="2009-07-28" version="1">MDEKVLVTGASGYIAMHIINILQSERYHVIATVGTPEDANKIAYSFEHYYPYAVLDVVVVEDVAAEDAFEEVFEQHPDIQHVIHTAPPALLGQGKTNEEAYLVPTREGTKNVLESIRKRGHNVKRVVVTSSFSALMDLDNAANPQCVITEKSWNPVTWEVAKDKESKAYAASKKIAEQLVWKFYEDNKDCVPYTLTTVNPPYVFGPHVFEWELGRAEWNTTALFIKKALEMSADEQGPFAKPRGICCDVRDAALLHILPLRNAALAGHRLFPVNGTGIKQNSYEDGKFNMQRIVDVLNARFPELQGKIPQGLPTENQQYMGQLTRYNNDNTCELTGMEFKSFETTVVDATRQIMRFQERHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>C5DD01</accession>
    <name>C5DD01_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">COP9 signalosome complex subunit 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0B07194g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="4">Component of the COP9 signalosome (CSN) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M67A family. CSN5 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552100.1">
        <property type="nucleotide sequence ID" value="XM_002552054.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DD01"/>
    <dbReference type="FunCoup" id="C5DD01">
        <property type="interactions" value="66"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DD01"/>
    <dbReference type="MEROPS" id="M67.A02"/>
    <dbReference type="GeneID" id="8290940"/>
    <dbReference type="KEGG" id="lth:KLTH0B07194g"/>
    <dbReference type="eggNOG" id="KOG1554">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031199_1_0_1"/>
    <dbReference type="InParanoid" id="C5DD01"/>
    <dbReference type="OMA" id="QMESYEY"/>
    <dbReference type="OrthoDB" id="605656at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008180">
        <property type="term" value="C:COP9 signalosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08069">
        <property type="entry name" value="MPN_RPN11_CSN5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000203">
        <property type="entry name" value="COP9 signalosome complex subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050242">
        <property type="entry name" value="JAMM_MPN+_peptidase_M67A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10410">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01398">
        <property type="entry name" value="JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00232">
        <property type="entry name" value="JAB_MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102712">
        <property type="entry name" value="JAB1/MPN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0736">Signalosome</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MPN" evidence="14">
        <location>
            <begin position="75"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR21662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="412" mass="47276" checksum="EE89061B7BCF2428" modified="2009-07-28" version="1">MRYSLETCRDLRARAHLVSQQLKDIEEGHGSMILGEREDCIDQPKSLGDLIRKQVGVDVREESEQKHNSRHFKRVMLSNMASYKILQHALKGGDVEIMGMLVGSTDRDSIIVFDCYPLPVEGTETRVNAQLESYEYMVQYMNEVYDSCSHPKNIVGWYHSHPGYGCWLSGIDVQTQELNQTFQDPYIAVVVDPKKSAEDKRLSIGAFRTLNEDEIPENVDQYGDSRYGHHSHKYYELEVKIFTSIFDTTLEQLQLRFDISASETPESSMVLEQLLESVRNWNNFRKLTNPNQFLVQNSRQSDFSVQDHEVDMGAEFSSFSRRSSRASSVTSSKDESTGSDVDMISNAIVGDYSTESSLPPASLAQPGMVTSEVEVPQVRSFEREYYLYKTNLSLIKLKEYERLRYFKDTFTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>C5DD15</accession>
    <name>C5DD15_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0B07524p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0B07524g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552114.1">
        <property type="nucleotide sequence ID" value="XM_002552068.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DD15"/>
    <dbReference type="GeneID" id="8290954"/>
    <dbReference type="KEGG" id="lth:KLTH0B07524g"/>
    <dbReference type="HOGENOM" id="CLU_1555530_0_0_1"/>
    <dbReference type="InParanoid" id="C5DD15"/>
    <dbReference type="OrthoDB" id="10490767at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="172" mass="19209" checksum="CE6F0302807BE3F4" modified="2009-07-28" version="1">MHHNKDKSSEARFSYLQLAVGPIKGSAAKERQQGNLTSDGQKREEHNATEQASIRDGKQASGLEPNREGRRKVQAWGGSKSKKTRSQEFMFKEIRKDPADSSILVDPNSDKLESAQAQLQLLFDDLAGDREGSAARQQSTIAKMQTDFNKIQHCEIDPRELDSAPSYSSARY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2010-10-05" modified="2025-06-18" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>C5DD42</accession>
    <name>PURA_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Adenylosuccinate synthetase</fullName>
            <shortName evidence="2">AMPSase</shortName>
            <shortName evidence="2">AdSS</shortName>
            <ecNumber evidence="2">6.3.4.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">IMP--aspartate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">KLTH0B08118g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plays an important role in the de novo pathway and in the salvage pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>IMP + L-aspartate + GTP = N(6)-(1,2-dicarboxyethyl)-AMP + GDP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:15753"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="6.3.4.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="2">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the adenylosuccinate synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.4" evidence="2"/>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552141.1">
        <property type="nucleotide sequence ID" value="XM_002552095.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DD42"/>
    <dbReference type="SMR" id="C5DD42"/>
    <dbReference type="FunCoup" id="C5DD42">
        <property type="interactions" value="865"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DD42"/>
    <dbReference type="GeneID" id="8290981"/>
    <dbReference type="KEGG" id="lth:KLTH0B08118g"/>
    <dbReference type="eggNOG" id="KOG1355">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029848_3_2_1"/>
    <dbReference type="InParanoid" id="C5DD42"/>
    <dbReference type="OMA" id="FHHAKPI"/>
    <dbReference type="OrthoDB" id="10265645at2759"/>
    <dbReference type="UniPathway" id="UPA00075">
        <property type="reaction ID" value="UER00335"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004019">
        <property type="term" value="F:adenylosuccinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044208">
        <property type="term" value="P:'de novo' AMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046040">
        <property type="term" value="P:IMP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03108">
        <property type="entry name" value="AdSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.170.10:FF:000001">
        <property type="entry name" value="Adenylosuccinate synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.300.10:FF:000002">
        <property type="entry name" value="Adenylosuccinate synthetase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.440.10">
        <property type="entry name" value="Adenylosuccinate Synthetase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.300.10">
        <property type="entry name" value="Adenylosuccinate Synthetase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.170.10">
        <property type="entry name" value="Adenylosuccinate Synthetase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00011">
        <property type="entry name" value="Adenylosucc_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018220">
        <property type="entry name" value="Adenylosuccin_syn_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033128">
        <property type="entry name" value="Adenylosuccin_syn_Lys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042109">
        <property type="entry name" value="Adenylosuccinate_synth_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042110">
        <property type="entry name" value="Adenylosuccinate_synth_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042111">
        <property type="entry name" value="Adenylosuccinate_synth_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001114">
        <property type="entry name" value="Adenylosuccinate_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002223">
        <property type="entry name" value="PRK01117.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00184">
        <property type="entry name" value="purA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11846">
        <property type="entry name" value="ADENYLOSUCCINATE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11846:SF0">
        <property type="entry name" value="ADENYLOSUCCINATE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00709">
        <property type="entry name" value="Adenylsucc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00788">
        <property type="entry name" value="Adenylsucc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01266">
        <property type="entry name" value="ADENYLOSUCCIN_SYN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00513">
        <property type="entry name" value="ADENYLOSUCCIN_SYN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000399340" description="Adenylosuccinate synthetase">
        <location>
            <begin position="1"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="12"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="40"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="11"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="2">
        <location>
            <begin position="12"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="2">
        <location>
            <begin position="37"/>
            <end position="40"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="39"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="2">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="2">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="2">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="305"/>
            <end position="311"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="2">
        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="311"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="337"/>
            <end position="339"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="419"/>
            <end position="421"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03125"/>
        </source>
    </evidence>
    <sequence length="434" mass="48259" checksum="8113DD5D3E5CAE32" modified="2009-07-28" version="1">MVNVVLGSQWGDEGKGKLVDLLVGKYDIVARAAGGNNAGHTIVVKGVKYDFHMLPSGLVNPNCQNLIGNGVVMHVPSFFAELEQLEAKGLTDARERLFISSRAHLVFDFHQRTDKLREAELSGASTDGKNIGTTGKGIGPTYATKASRSGLRVHHLVNDEPGAWEEFETKYRRLVQTRQQRYGDFEYDADAELARYKKYREELKPFVVDSVVFMHNAIRDNKKILVEGANALMLDIDFGTYPYVTSSNTGIGGVCTGLGLPPRVIDEVYGVVKAYTTRVGEGPFPTEQLNESGEKLQNIGAEFGVTTGRKRRCGWLDLVVLKYSTLINGYTSLNITKLDVLDTFKEIPVGVSYTLRGKKLDLFPEDLNVLGKVDVEYVTLPGWEQDITKITEYEDLPENAKKYLKFIEDFVGVPIEWVGTGPARESMVYKQVSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>C5DD56</accession>
    <name>C5DD56_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0B08448p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0B08448g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552155.1">
        <property type="nucleotide sequence ID" value="XM_002552109.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DD56"/>
    <dbReference type="FunCoup" id="C5DD56">
        <property type="interactions" value="97"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DD56"/>
    <dbReference type="GeneID" id="8290995"/>
    <dbReference type="KEGG" id="lth:KLTH0B08448g"/>
    <dbReference type="eggNOG" id="KOG4339">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_312616_0_0_1"/>
    <dbReference type="InParanoid" id="C5DD56"/>
    <dbReference type="OMA" id="NYHEDIT"/>
    <dbReference type="OrthoDB" id="5563016at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12751:SF18">
        <property type="entry name" value="PHOSPHATASE AND ACTIN REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12751">
        <property type="entry name" value="PHOSPHATASE AND ACTIN REGULATOR PHACTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="164"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="312"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="501"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="662"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="738"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="194"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="240"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="265"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="282"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="317"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="358"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="394"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="510"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="662"/>
            <end position="679"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="864" mass="95542" checksum="927FBDA7B9EC7EAD" modified="2009-07-28" version="1">MNQSLEEANNDGESYLNDTFYSSTSTDNLEHARTFRNSVLMQDMSGEKPYNPEQEQIPSDPSDTQGKQYLKSNTNDANDRETETKTLTVTASPSLSALAGILNEKAIQAEKKMRSSMILDNSIQESIPEEEHSVHNSPVLIDIDGTSDGSYRLKFPPQMAHLQNEIEQPDFLTTPRLDPPTPRVEPADSDLPNGGQQEKPQEQVSNVSSEREIETQNSNSPKATAERADNSRPLPRKQASLRSFSGMSVGSFASNEQPLKESSSGKKRKSIFSFLKKKPRKNSSLLSQDNSSSATLPTSATFSVSKSYHENDLPASTRLTKNSPSNGSIFSTFRKNKASKKEAAAAADRTLHVPKQRATSGDSNSSNTGGASQQKRGVQTRKPTPLDFEHVSQKAPSTQHNSPILTEPSTELQNTPQLVVKSPVPSENKVPDDVGESLLKADSGGVLFPKSLSAHEVESIVSLERSRSVKSAKRNSLNSHRVSLTDNMSAKINNDGMFITEPSAPKLSTPDLTKSPTSSILRNGTFDTLESSTQRGSYAEPNNLGISSAGLRAQDRDFSFTSIEQKLNDITLDSESEDELAVNTKKASTSAGENFDTELMSDIMEFANIIDFGKDLDLNLDLSQEDINYKSLNPSDVKQSEKLPRDEMSADSFNFNSVAQVPLSSSGDRQSFSQHSYRSSIERNDTTNTDRTSFRRHSTLLSPPLNEDTRTEQMRNILSADDDDDEFEGEDFNELEEKLESQTIGSPTWQPPLDNRNRPISISFKGLRANQLNSSSNLSALRLSPSDYTVSNSHDLMSERKSVSFSSSIVLFSTYTEDEYDRHPEVATCNQLTPQLAQIIKEELNTLKAEMEVHEESRCYTHFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>C5DD88</accession>
    <name>C5DD88_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">rRNA-processing protein EFG1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">rRNA-processing protein efg1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0B09218g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EFG1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552187.1">
        <property type="nucleotide sequence ID" value="XM_002552141.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DD88"/>
    <dbReference type="FunCoup" id="C5DD88">
        <property type="interactions" value="199"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DD88"/>
    <dbReference type="GeneID" id="8291030"/>
    <dbReference type="KEGG" id="lth:KLTH0B09218g"/>
    <dbReference type="eggNOG" id="KOG4484">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066912_2_0_1"/>
    <dbReference type="InParanoid" id="C5DD88"/>
    <dbReference type="OMA" id="KPHRIQE"/>
    <dbReference type="OrthoDB" id="47732at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019310">
        <property type="entry name" value="Efg1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050786">
        <property type="entry name" value="EFG1_rRNA-proc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33911">
        <property type="entry name" value="RRNA-PROCESSING PROTEIN EFG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33911:SF1">
        <property type="entry name" value="RRNA-PROCESSING PROTEIN EFG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10153">
        <property type="entry name" value="Efg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="187"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="53"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="211"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR21749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="222" mass="25581" checksum="884AFDAF387C2F0B" modified="2009-07-28" version="1">MDRKQRRRTNKSAGAPIQLARVLGSGSNKIKRKIRDIERLLQKKKDVLPDTVLIEKERALEALRLELHNAEQRVKIQNNAKKYHMVRFFERKKALRRYTKSVKEGDADAVRERSIDLCYVVNFPKTEKYIALYPSEESESRDTDDKKGVAKTEARRAQFRDLVAAKMDAGTLPVPLKDILKGKKLDRDDTGVQLETGESRASKEASISESEKEDEDEDDFFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDA8</accession>
    <name>C5DDA8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">glucan 1,3-beta-glucosidase</fullName>
            <ecNumber evidence="11">3.2.1.58</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Exo-1,3-beta-glucanase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">KLTH0B09658g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>Successive hydrolysis of beta-D-glucose units from the non-reducing ends of (1-&gt;3)-beta-D-glucans, releasing alpha-glucose.</text>
            <dbReference type="EC" id="3.2.1.58"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the glycosyl hydrolase 17 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.58" evidence="11"/>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552207.1">
        <property type="nucleotide sequence ID" value="XM_002552161.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDA8"/>
    <dbReference type="FunCoup" id="C5DDA8">
        <property type="interactions" value="480"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDA8"/>
    <dbReference type="GeneID" id="8291050"/>
    <dbReference type="KEGG" id="lth:KLTH0B09658g"/>
    <dbReference type="eggNOG" id="ENOG502QQE6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028820_2_0_1"/>
    <dbReference type="InParanoid" id="C5DDA8"/>
    <dbReference type="OMA" id="EGICAMR"/>
    <dbReference type="OrthoDB" id="1293114at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004338">
        <property type="term" value="F:glucan exo-1,3-beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000105">
        <property type="entry name" value="Glucan 1,3-beta-glucosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050732">
        <property type="entry name" value="Beta-glucan_modifiers"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000490">
        <property type="entry name" value="Glyco_hydro_17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16631">
        <property type="entry name" value="GLUCAN 1,3-BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16631:SF26">
        <property type="entry name" value="GLUCAN 1,3-BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00332">
        <property type="entry name" value="Glyco_hydro_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00587">
        <property type="entry name" value="GLYCOSYL_HYDROL_F17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0134">Cell wall</keyword>
    <keyword evidence="9" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 15" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002947780" description="glucan 1,3-beta-glucosidase" evidence="15">
        <location>
            <begin position="20"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00036824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00041761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAR21769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="307" mass="33142" checksum="100DC89E054313DE" modified="2009-07-28" version="1">MKFSTIASLAAVGVSYASAVGDLGFNLGVKNNDGTCKSTEDYAKDLDILKGYTSTVKVYAASDCNTLQNLGPAAESAGFSVFVGVWPNDDAHFSAEKQALQSYLPNIKTSTVKGILVGSEALYREDLTASELASKISEVKSLVADIKDSDGNSYSGTLVGTVDSWNVLVDGGNADVIKASDMVMANAFSYWQGQTMDNASYSFFDDIMQAMQTIQTVKGTDIPFWVGETGWPTDGTNFESSYPGTDNAAQFWQEGICAIRAWGVNVLVFEAFDEDWKPNTSGTSDVEKHWGVWTSGDELKYSLDCKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDM9</accession>
    <name>C5DDM9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GPN-loop GTPase</fullName>
            <ecNumber evidence="7">3.6.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0C02310g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Small GTPase required for proper nuclear import of RNA polymerase II (RNAPII). May act at an RNAP assembly step prior to nuclear import.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Binds to RNA polymerase II.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the GPN-loop GTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.-" evidence="7"/>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552328.1">
        <property type="nucleotide sequence ID" value="XM_002552282.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDM9"/>
    <dbReference type="FunCoup" id="C5DDM9">
        <property type="interactions" value="953"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDM9"/>
    <dbReference type="GeneID" id="8291188"/>
    <dbReference type="KEGG" id="lth:KLTH0C02310g"/>
    <dbReference type="eggNOG" id="KOG1532">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037460_1_1_1"/>
    <dbReference type="InParanoid" id="C5DDM9"/>
    <dbReference type="OMA" id="MIIVFNK"/>
    <dbReference type="OrthoDB" id="243313at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17870">
        <property type="entry name" value="GPN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000579">
        <property type="entry name" value="GPN-loop GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004130">
        <property type="entry name" value="Gpn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030230">
        <property type="entry name" value="Gpn1/Npa3/XAB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231:SF8">
        <property type="entry name" value="GPN-LOOP GTPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231">
        <property type="entry name" value="XPA-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03029">
        <property type="entry name" value="ATP_bind_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="263"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR21890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="385" mass="43429" checksum="9A6C90D69E2023CF" modified="2009-07-28" version="1">MPLKTVICIGMAGSGKTTFMQRLNSHLHAAKSPPYVVQLDPAVLNVPYGANIDIRDSVKYKKVMENYNLGPNGAIVTSLNLFSTKIDQVIKLVENKRDKYEHCIIDTPGQIECFVWSASGAIITESFASTFPTVIAYIVDTPRNSSPTTFMSNMLYACSILYKTKLPMVVVFNKTDVCKADFAREWMTDFEAFQDALRQDQELNGETGMGSGYMGSLVNSMSLMLEEFYSQLDMVGVSSYTGEGFDEFLTAVDKKVDEYESYYKAERERILKQKEEEEKTKKQKSLEHLMKDLGLKDEQNAENAKQEEADVLSDFEEGENDGIVLRDEDEGVEREYTFAEGDRMSGEVNGKSTPDLQKRYEEAFQKVAKSASSETAENIARYIRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDN1</accession>
    <name>C5DDN1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Deoxyhypusine hydroxylase</fullName>
            <shortName evidence="9">DOHH</shortName>
            <ecNumber evidence="9">1.14.99.29</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Deoxyhypusine dioxygenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Deoxyhypusine monooxygenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LIA1</name>
        <name evidence="10" type="ordered locus">KLTH0C02354g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the hydroxylation of the N(6)-(4-aminobutyl)-L-lysine intermediate to form hypusine, an essential post-translational modification only found in mature eIF-5A factor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>[eIF5A protein]-deoxyhypusine + AH2 + O2 = [eIF5A protein]-hypusine + A + H2O</text>
            <dbReference type="Rhea" id="RHEA:14101"/>
            <dbReference type="Rhea" id="RHEA-COMP:10144"/>
            <dbReference type="Rhea" id="RHEA-COMP:12592"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:82657"/>
            <dbReference type="ChEBI" id="CHEBI:91175"/>
            <dbReference type="EC" id="1.14.99.29"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="9">Binds 2 Fe(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Protein modification; eIF5A hypusination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the deoxyhypusine hydroxylase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.99.29" evidence="9"/>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552330.1">
        <property type="nucleotide sequence ID" value="XM_002552284.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDN1"/>
    <dbReference type="FunCoup" id="C5DDN1">
        <property type="interactions" value="968"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDN1"/>
    <dbReference type="GeneID" id="8291190"/>
    <dbReference type="KEGG" id="lth:KLTH0C02354g"/>
    <dbReference type="eggNOG" id="KOG0567">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053974_0_0_1"/>
    <dbReference type="InParanoid" id="C5DDN1"/>
    <dbReference type="OMA" id="LQEPCSI"/>
    <dbReference type="OrthoDB" id="421002at2759"/>
    <dbReference type="UniPathway" id="UPA00354"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019135">
        <property type="term" value="F:deoxyhypusine monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000099">
        <property type="entry name" value="Deoxyhypusine hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03101">
        <property type="entry name" value="Deoxyhypusine_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027517">
        <property type="entry name" value="Deoxyhypusine_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004155">
        <property type="entry name" value="PBS_lyase_HEAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12697:SF5">
        <property type="entry name" value="DEOXYHYPUSINE HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12697">
        <property type="entry name" value="PBS LYASE HEAT-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13646">
        <property type="entry name" value="HEAT_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00567">
        <property type="entry name" value="EZ_HEAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 9" id="KW-0386">Hypusine biosynthesis</keyword>
    <keyword evidence="6 9" id="KW-0408">Iron</keyword>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR21892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="322" mass="36096" checksum="DE529D2D9865B576" modified="2009-07-28" version="1">MSTNFEKHFEENVDNCSLEQLRDILINKSGNSKLANRFRALFNLKGIAEEFEKSPEEALKATKYISECFGDSSELLKHEVAYVLGQTKNMAGAPILRDVLADNDQQCMVRHEAAEALGALGDVDSLPILETYFKEDPLLEIRQTCELAIGRIKWQHSGLADKENLQESLYSSIDPAPPLTKDTDYKIGELKQILLDQEKPLFERYRAMFRLRDIGNDEACLALAAGFDDPSALFKHEVAYVFGQIGNSVVVPHLVEVLGRENEAPMVRHEAAEALGSIATYDVLPVLKDHLTDKDDVVRESAIVALDMYEYENSNELEYAPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDR0</accession>
    <name>C5DDR0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0C03036p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0C03036g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552359.1">
        <property type="nucleotide sequence ID" value="XM_002552313.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDR0"/>
    <dbReference type="FunCoup" id="C5DDR0">
        <property type="interactions" value="47"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDR0"/>
    <dbReference type="GeneID" id="8291221"/>
    <dbReference type="KEGG" id="lth:KLTH0C03036g"/>
    <dbReference type="eggNOG" id="KOG1947">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006598_2_1_1"/>
    <dbReference type="InParanoid" id="C5DDR0"/>
    <dbReference type="OMA" id="MCGQLNN"/>
    <dbReference type="OrthoDB" id="1924287at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318:SF247">
        <property type="entry name" value="GH16156P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318">
        <property type="entry name" value="PARTNER OF PAIRED, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="600" mass="67964" checksum="61F5261900FB8B6A" modified="2009-07-28" version="1">MYRGRNRSKTNKDADDGSVRGPNSALTQFLKEQGISAESIRQRWLKSREKEGGSETDGVKKEGSEEDERSLSASTRPKSEESGTESGDEYDRSDEENEEDNDDDADETESQEITRSRLKANGSPRENRLKRFAAESEDSDEEEYDEGMSSRTISETPVPLQQEDHALKIKKSKQQLQQRRRKKKRAAELLDRKTRRISTLQDICISQISANISKLQKETSSSDETLSQQIRQTLGGISTENMNKLARTLSKNRALNDHTLQLFLRTDLESLTFHDCSKVSYEGYKLLAIFAPHLSELSLQMCGQLNNEALLYIAQKLPNLNALYLDGPFLINESTWDEFFSLMKNRLKAFHVSNTHRFTDHSLRNLLENCGESLVSLRLSRLDAVFDYSLVSQYLRNPNFEHLSLEYPNKEELVSDDIVIDALKQVGPTLKHLSLNGCFALTDATFISAKDALSSENSHSSMLENLQLEELDQITTDGMVYFFSNIQMPNLKNCSLRRCFQLQDMAVSELFLNEASKSLTDLSLNSLKDVKGECFEAMECPKLKRLDVGFMGCVNDKLVKNLGERNPDLEILEVFGDNLVSRNAVIRPGLTVIGRQSDTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDR1</accession>
    <name>C5DDR1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0C03102p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0C03102g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the putative lipase ROG1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552360.1">
        <property type="nucleotide sequence ID" value="XM_002552314.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDR1"/>
    <dbReference type="GeneID" id="8291224"/>
    <dbReference type="KEGG" id="lth:KLTH0C03102g"/>
    <dbReference type="eggNOG" id="KOG4372">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027968_1_1_1"/>
    <dbReference type="InParanoid" id="C5DDR1"/>
    <dbReference type="OMA" id="FCTPHVG"/>
    <dbReference type="OrthoDB" id="273452at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007751">
        <property type="entry name" value="DUF676_lipase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044294">
        <property type="entry name" value="Lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482:SF65">
        <property type="entry name" value="ESTERASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G12320)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482">
        <property type="entry name" value="LIPASE ROG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05057">
        <property type="entry name" value="DUF676"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="300"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="DUF676" evidence="4">
        <location>
            <begin position="13"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR21922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="525" mass="59370" checksum="08206710FDBAAB0D" modified="2009-07-28" version="1">MLEPAVDKTGSTAYHLVVLVHGLWGNRSHLEYISNALKTEFDSRNRSNSGEQLFVYTAHLNEGYKTYDGIDVCGVRVASEIEEQISALGSVTKFSICGYSLGGLISRYALGVLYKRQVFKKRDIKLVNFTTFCTPHVGVYAPGKNAAVKLFNAVVPLVLGNSGKQMFLKDKSKLAGGLPLVLAMSMENSVFYKALQEFESKSLYANVINDKRTAWWTSGISRNDPFFDIDENNGHHRFCYVSGYGPIVVDHTKPVKISRIEDFNERAEEKARPRQSGENMKVDKQEYFFLNYWIAKLGRWLMVLINLLLIAPLWALWFVISGTTETIKSTIRVTKFVKGYSHQFIQEIYDIPSSPDHEDDMIETLPDTPGEYELQFQQSLQDQTDTFIESVLSAIERKNTLSAVVDEAEPCDEKNMSDEGDTGKDFSMRTTLKELELYSVEAIKARHSRSASNEKLPSLENLHLELGPAQLQIIDSLNKIPWSKFPVYIRNTPSTHACAIVRHGDPKFGEGKVIVKHWVSEVFSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDX6</accession>
    <name>C5DDX6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0C04620p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0C04620g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552425.1">
        <property type="nucleotide sequence ID" value="XM_002552379.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDX6"/>
    <dbReference type="FunCoup" id="C5DDX6">
        <property type="interactions" value="141"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDX6"/>
    <dbReference type="GeneID" id="8291292"/>
    <dbReference type="KEGG" id="lth:KLTH0C04620g"/>
    <dbReference type="eggNOG" id="ENOG502R4NW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_446963_0_0_1"/>
    <dbReference type="InParanoid" id="C5DDX6"/>
    <dbReference type="OMA" id="LWNGCEC"/>
    <dbReference type="OrthoDB" id="3976101at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR21987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="548" mass="63475" checksum="843C47F8FD51A0E7" modified="2009-07-28" version="1">MTSREMHDGLWPAVMEFLNQNDLLILAQTSKKLSKVSLQNLYGRIVISKEPVMRSDEWFLDCSANYVSGYRSTKKTPDQNDIFLYDRIKRLTESSHLALVKELVIQEDVFYDRESGLPVLQALIDRLLELDQVEVLDIRDSALFEHNYSKILELTHLRKCRVVNIGDLGKLRSLPRIKSLEISLTHPDFKPRCLSDDVKKSLSDTVVELTVDDLEHSSLRLFQYFHKEGMRLGHVTSLKFNHVHGIHDYNKTMRELTTIFLKDVFDLKKIESLELEGSCEASDCTCFNDFLMDMAPELVSVRSLGLIEKNFVTQGDHNTEEEWDLTINRFILHIPKVSERLKNLTVRHNPPLNGITVDSVEGNYIRRRTLYDNVLPILTNLQTLVGPTFLKSLSAYEILVCDLLWNGCECSFCAKVLPVIDQYIMNHQYYSFPDGSFKDVIPTVFFAYTGDALSRRFITETDWDLKTLATCPVSHVWDLHGYESLQHFKNFDCFFDESVFMALTKCVSHFFNTYMDHLVKFMPNMRTCVLSGVYYSVDEQGKYKSIYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-04-02" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDZ1</accession>
    <name>C5DDZ1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0C04950p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0C04950g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552440.1">
        <property type="nucleotide sequence ID" value="XM_002552394.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDZ1"/>
    <dbReference type="STRING" id="559295.C5DDZ1"/>
    <dbReference type="GeneID" id="8291307"/>
    <dbReference type="KEGG" id="lth:KLTH0C04950g"/>
    <dbReference type="eggNOG" id="ENOG502S2KG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_450603_0_0_1"/>
    <dbReference type="InParanoid" id="C5DDZ1"/>
    <dbReference type="OMA" id="YHRFACY"/>
    <dbReference type="OrthoDB" id="418911at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="379"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="16"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR22002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="624" mass="68448" checksum="D2092610A0034C53" modified="2009-07-28" version="1">MSALNQKYKKPRLQQAETAGAVAPAAPATPEAPETPATPAKYAATAESSVLDAALSDADSALGESYAACGRLAATMGLHQLAVVNLAKSMQKLPRDVGVLVDFAKALGAKANQESIVVSTLADAVQRSPELSDPRVWLQLASSYYHLLKPDEAFHSVSRAIAAQEIQGIKDAGTWALQARILLLWMDSDAKMTLETLTPYFIGAIEMAIAAGDTQLELQSRVSLAQLYHRFACYPESQAEVSRAFVLVRSDPSSGMPRPSTVNTLCYLYNFLGIIQFKMNYKASAYSVIQEAMSALPQVSATARLLATLAQFYMIDENLPQLRAILPALLVEKTALTEKELIRLYLMNWLLGRVYDMLDDTKQSYHFYQQAVNCKPQSASLWVGIGSLYLRMRQFEDAHIAFSHALNYASKLENFEQPFFLRFNRLFAAFAWVGLSQVFVATFQKQSALDALRQASVLFTAEGDLVHSSQVDQLYNDVLATPPQNPMYVIMNVPPQILLELFLYYDTGVFANQTEGNFPKISSCEPVAHIMPLYDHQASPVATLPHGPAVTPSVPPPPPQQVYNDLATRPALSQQHPSTVFLAYPQPAVTVPATSALNIKPIAKNMPGVNYGFPAQPYQNPPVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEI0</accession>
    <name>C5DEI0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0C09394p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0C09394g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552629.1">
        <property type="nucleotide sequence ID" value="XM_002552583.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEI0"/>
    <dbReference type="FunCoup" id="C5DEI0">
        <property type="interactions" value="1059"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEI0"/>
    <dbReference type="GeneID" id="8291507"/>
    <dbReference type="KEGG" id="lth:KLTH0C09394g"/>
    <dbReference type="eggNOG" id="KOG2050">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013994_1_0_1"/>
    <dbReference type="InParanoid" id="C5DEI0"/>
    <dbReference type="OMA" id="YGPEFSI"/>
    <dbReference type="OrthoDB" id="497380at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010629">
        <property type="term" value="P:negative regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012959">
        <property type="entry name" value="CPL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033133">
        <property type="entry name" value="PUM-HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040059">
        <property type="entry name" value="PUM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13389">
        <property type="entry name" value="PUMILIO HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13389:SF0">
        <property type="entry name" value="PUMILIO HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08144">
        <property type="entry name" value="CPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50303">
        <property type="entry name" value="PUM_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="PUM-HD" evidence="5">
        <location>
            <begin position="132"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="3">
        <location>
            <begin position="153"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="3">
        <location>
            <begin position="190"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="3">
        <location>
            <begin position="226"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="59"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="87"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="103"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR22191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="657" mass="74704" checksum="9F4CBBD00BAC48B4" modified="2009-07-28" version="1">MAPVISKKSGKKHSLNAKDQKTAKKAKLSSKVPEDEGVSGSSSEEEDDLDEVLSSGESDKDELDESSSEGEQEDGSEGSEDESEGSSEAKGEDDSKGGVSQHAEQRKLLKERKLQRKSGTQVQQIKSLWERLRVKNPPMPKEVREKLSNEIWELSKDCISDLVMKHDASRVVQTLVKYSSKARRDQIVAALKGQYYVLATSSYGKYLLVKLLHYGSKQSRQLIIDELHGSLRKLMRHREGAYVVEDLYVLYASHEQKQQMLREFWGSEYAVFRDAHKNQTLEQVCESSAEKKTIIARNLIGTISASVEKGSTGFQILHAAMRDYVKIAGEKETSEFIELLDEKYAELVHTPEGADVACTLLAKATAKERKMIIKNLKDHADKLIRNEHGNLVFITALMCVDDTVLMFKSFGPSVKEMLQSFVVDKYGRRPFLYILLGLDGKYFSPIVKKDLNRYIEYSKATSKKPFEQRRKELLNKFAPMYLSTISKYYDMILSDNLGSQFVSEVLVNDEFYDQLSDKEKEKHREIVNTVSTYFKGDVSEESHPIHKPFSVRLLKSLIQGGKWNAKEKKVEPLHSVLGLGTEFAQQFYEDIIDETNLLDWINNADSSFTIVAIYESLSKTENKKFFKDLKKVSKKINKDDESNKGARLLLKLMNNKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEN3</accession>
    <name>C5DEN3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Histone H2A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0C10648g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="9">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the histone H2A family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552682.1">
        <property type="nucleotide sequence ID" value="XM_002552636.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEN3"/>
    <dbReference type="FunCoup" id="C5DEN3">
        <property type="interactions" value="1247"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEN3"/>
    <dbReference type="GeneID" id="8291563"/>
    <dbReference type="KEGG" id="lth:KLTH0C10648g"/>
    <dbReference type="eggNOG" id="KOG1757">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062828_2_1_1"/>
    <dbReference type="InParanoid" id="C5DEN3"/>
    <dbReference type="OMA" id="MNKKGAP"/>
    <dbReference type="OrthoDB" id="9421954at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000791">
        <property type="term" value="C:euchromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00074">
        <property type="entry name" value="HFD_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000021">
        <property type="entry name" value="Histone H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002119">
        <property type="entry name" value="Histone_H2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032454">
        <property type="entry name" value="Histone_H2A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032458">
        <property type="entry name" value="Histone_H2A_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23430">
        <property type="entry name" value="HISTONE H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16211">
        <property type="entry name" value="Histone_H2A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00620">
        <property type="entry name" value="HISTONEH2A"/>
    </dbReference>
    <dbReference type="SMART" id="SM00414">
        <property type="entry name" value="H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00046">
        <property type="entry name" value="HISTONE_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4 9" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 9" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="11">
        <location>
            <begin position="24"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="Histone H2A C-terminal" evidence="12">
        <location>
            <begin position="100"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF16211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR22244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="134" mass="14354" checksum="2787E0321CDB38C5" modified="2009-07-28" version="1">MSGKVHGGKGKSGAKTGSNMRATTHSARAGLQFPVGRIKRYLKRNAAGRTRVGSKSAIYLTAVLEYLTAEVLELAGNAAKDLKVKRITPRHLQLAIRGDDELDTLVRATIASGGVLPHINKALLLKVERKGTKN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-06-28" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEU6</accession>
    <name>GEP3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName>Genetic interactor of prohibitins 3, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Found in mitochondrial proteome protein 38</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">GEP3</name>
        <name type="synonym">FMP48</name>
        <name type="ordered locus">KLTH0D09878g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in the mitochondrial lipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class YlqF/YawG GTPase family. GEP3 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553139.1">
        <property type="nucleotide sequence ID" value="XM_002553093.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEU6"/>
    <dbReference type="SMR" id="C5DEU6"/>
    <dbReference type="FunCoup" id="C5DEU6">
        <property type="interactions" value="109"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEU6"/>
    <dbReference type="GeneID" id="8295378"/>
    <dbReference type="KEGG" id="lth:KLTH0D09878g"/>
    <dbReference type="eggNOG" id="ENOG502S0UP">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025792_1_0_1"/>
    <dbReference type="InParanoid" id="C5DEU6"/>
    <dbReference type="OMA" id="IIPPFYG"/>
    <dbReference type="OrthoDB" id="1696305at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030378">
        <property type="entry name" value="G_CP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050896">
        <property type="entry name" value="Mito_lipid_metab_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46434">
        <property type="entry name" value="GENETIC INTERACTOR OF PROHIBITINS 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46434:SF1">
        <property type="entry name" value="GENETIC INTERACTOR OF PROHIBITINS 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51721">
        <property type="entry name" value="G_CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0496">Mitochondrion</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0809">Transit peptide</keyword>
    <feature type="transit peptide" description="Mitochondrion" evidence="2">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000409636" description="Genetic interactor of prohibitins 3, mitochondrial">
        <location>
            <begin position="39"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="domain" description="CP-type G" evidence="3">
        <location>
            <begin position="111"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01058"/>
        </source>
    </evidence>
    <sequence length="535" mass="60679" checksum="5F9E39C577B722FA" modified="2009-07-28" version="1" precursor="true">MLPVSRLCVRSSLRKLVFVRFVSCTSCGVTLQNNNVRGTGFYTPPKAQIERKRPVIEDLKYLLFSQDLQSLKSELSAEERPKKNKPLICKRCNDALHHNTFSKEDFRRYSMQEVYRHVPAGADIYHVVPLTDFPLQLNSAVLKDSRYNSALLLSKGDQVTPDKSLLQRKAPQFFKDMLRLKMNYNSNKSIAFSASKGWNIQSVYSVLRSNSFLIGCPNSGKSTLVNALLKKFPSLKPSEGNNASEAEIVRGAGVSNIPNMTRDLQSYNIGSKVINDLPGYSTNYGSPGPEDALDAKVLEKIQKTHLFKKTKLVKQRYTSLKGTDAGRCYTVSGIFYLVPPPDTINQVVNYIPGNERQYRNIDKALSVVNSEHACPEKGPLRQYVGVQKAMESKDNYVRHVLPPFQGSIEVVLKEIGYFQLKTTGKYKFLGLHEVWVPKGVDVCIREPIARLIDEGYEGYLSSEGKKKAIPEKREVFSATYPMSFEETDTLQKMKEMFLERTKNDVLARKFISSNPLKIIGTAQKEAPNLYWYYKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DF24</accession>
    <name>C5DF24_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Autophagy-related protein 29</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0D11660g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2012" name="Cell" volume="151" first="1501" last="1512">
            <title>Architecture of the Atg17 complex as a scaffold for autophagosome biogenesis.</title>
            <authorList>
                <person name="Ragusa M.J."/>
                <person name="Stanley R.E."/>
                <person name="Hurley J.H."/>
            </authorList>
            <dbReference type="PubMed" id="23219485"/>
            <dbReference type="DOI" id="10.1016/j.cell.2012.11.028"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (3.06 ANGSTROMS) OF 1-40</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="journal article" date="2014" name="Nat. Struct. Mol. Biol." volume="21" first="513" last="521">
            <title>Structural basis of starvation-induced assembly of the autophagy initiation complex.</title>
            <authorList>
                <person name="Fujioka Y."/>
                <person name="Suzuki S.W."/>
                <person name="Yamamoto H."/>
                <person name="Kondo-Kakuta C."/>
                <person name="Kimura Y."/>
                <person name="Hirano H."/>
                <person name="Akada R."/>
                <person name="Inagaki F."/>
                <person name="Ohsumi Y."/>
                <person name="Noda N.N."/>
            </authorList>
            <dbReference type="PubMed" id="24793651"/>
            <dbReference type="DOI" id="10.1038/nsmb.2822"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (3.20 ANGSTROMS) OF 1-43</scope>
    </reference>
    <reference evidence="12" key="4">
        <citation type="journal article" date="2016" name="Dev. Cell" volume="38" first="86" last="99">
            <title>The Intrinsically Disordered Protein Atg13 Mediates Supramolecular Assembly of Autophagy Initiation Complexes.</title>
            <authorList>
                <person name="Yamamoto H."/>
                <person name="Fujioka Y."/>
                <person name="Suzuki S.W."/>
                <person name="Noshiro D."/>
                <person name="Suzuki H."/>
                <person name="Kondo-Kakuta C."/>
                <person name="Kimura Y."/>
                <person name="Hirano H."/>
                <person name="Ando T."/>
                <person name="Noda N.N."/>
                <person name="Ohsumi Y."/>
            </authorList>
            <dbReference type="PubMed" id="27404361"/>
            <dbReference type="DOI" id="10.1016/j.devcel.2016.06.015"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (3.21 ANGSTROMS) OF 1-87</scope>
    </reference>
    <comment type="interaction">
        <interactant intactId="EBI-6558893">
            <id>C5DF24</id>
        </interactant>
        <interactant intactId="EBI-6558887">
            <id>C5DEB9</id>
            <label>KLTH0C07942g</label>
        </interactant>
        <organismsDiffer>false</organismsDiffer>
        <experiments>7</experiments>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Preautophagosomal structure</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATG29 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553217.1">
        <property type="nucleotide sequence ID" value="XM_002553171.1"/>
    </dbReference>
    <dbReference type="PDB" id="4HPQ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.06 A"/>
        <property type="chains" value="A/D=1-40"/>
    </dbReference>
    <dbReference type="PDB" id="4P1W">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.20 A"/>
        <property type="chains" value="A/D=1-43"/>
    </dbReference>
    <dbReference type="PDB" id="5JHF">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.21 A"/>
        <property type="chains" value="A/D=1-87"/>
    </dbReference>
    <dbReference type="PDBsum" id="4HPQ"/>
    <dbReference type="PDBsum" id="4P1W"/>
    <dbReference type="PDBsum" id="5JHF"/>
    <dbReference type="AlphaFoldDB" id="C5DF24"/>
    <dbReference type="SMR" id="C5DF24"/>
    <dbReference type="DIP" id="DIP-60847N"/>
    <dbReference type="FunCoup" id="C5DF24">
        <property type="interactions" value="82"/>
    </dbReference>
    <dbReference type="IntAct" id="C5DF24">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DF24"/>
    <dbReference type="GeneID" id="8295457"/>
    <dbReference type="KEGG" id="lth:KLTH0D11660g"/>
    <dbReference type="eggNOG" id="ENOG502S1W0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121102_0_0_1"/>
    <dbReference type="InParanoid" id="C5DF24"/>
    <dbReference type="OMA" id="RKSEINW"/>
    <dbReference type="OrthoDB" id="21072at2759"/>
    <dbReference type="EvolutionaryTrace" id="C5DF24"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039113">
        <property type="entry name" value="ATG29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040666">
        <property type="entry name" value="Atg29_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039362">
        <property type="entry name" value="ATG29_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40012">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40012:SF1">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18388">
        <property type="entry name" value="ATG29_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="10 11 12" id="KW-0002">3D-structure</keyword>
    <keyword evidence="6" id="KW-0072">Autophagy</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Atg29 N-terminal" evidence="7">
        <location>
            <begin position="7"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF18388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR22779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="10">
        <source>
            <dbReference type="PDB" id="4HPQ"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="11">
        <source>
            <dbReference type="PDB" id="4P1W"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="12">
        <source>
            <dbReference type="PDB" id="5JHF"/>
        </source>
    </evidence>
    <sequence length="171" mass="19760" checksum="B1E3954CF6C71D45" modified="2009-07-28" version="1">MNSENTIVYVRVAGRARNGFVDPLKFYWDLERDRSLWSSVSKLDNTKKTIDWKRLSREFKAPEHFIRKRSYALFAKHLKLLERQIEAKTRSLAVTEDELNYTDEDDERNSISHGEDDFEAAEGLKNLRTSKVLKQRVPGEGRNGESSLSELSNLSVSKSALEEALLDRLQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DF42</accession>
    <name>C5DF42_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">26S proteasome regulatory subunit RPN8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0D12056g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M67A family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553235.1">
        <property type="nucleotide sequence ID" value="XM_002553189.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DF42"/>
    <dbReference type="FunCoup" id="C5DF42">
        <property type="interactions" value="1151"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DF42"/>
    <dbReference type="MEROPS" id="M67.973"/>
    <dbReference type="GeneID" id="8295475"/>
    <dbReference type="KEGG" id="lth:KLTH0D12056g"/>
    <dbReference type="eggNOG" id="KOG1556">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027018_3_0_1"/>
    <dbReference type="InParanoid" id="C5DF42"/>
    <dbReference type="OMA" id="HAMSIKT"/>
    <dbReference type="OrthoDB" id="10256771at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08062">
        <property type="entry name" value="MPN_RPN7_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000004">
        <property type="entry name" value="26S proteasome regulatory subunit rpn-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024969">
        <property type="entry name" value="EIF3F/CSN6-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033858">
        <property type="entry name" value="MPN_RPN7_8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10540:SF7">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10540">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01398">
        <property type="entry name" value="JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13012">
        <property type="entry name" value="MitMem_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00232">
        <property type="entry name" value="JAB_MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0647">Proteasome</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MPN" evidence="6">
        <location>
            <begin position="45"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="337"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="343"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00072102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR22797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="370" mass="40563" checksum="2EB8192749CD508B" modified="2009-07-28" version="1">MSMKGSQIKGGKLTIKLGTFEYNSPGGSQAHNRASGIMSLNHDSVTVAPLVLLSVLDHYRRINTPQGKRCVGVILGDSSSSTVRVTNSFALPFEEDEKNPDVWFLDHNYIESMNEMCKKINAKEKLVGWYHSGPKLKSADLKINDVFKKYTSSNPLLLIVDVKQESVGLPTTAYMAVEQVKDDGTSTEKTFLHLPSSIEAEEAEEIGVEHLLRDVRDQAAGGLSIKLTNQLKSLQGLQTKLSGIASYLTKVSEGQLPVNHVILGKLQDVFNLLPNLGVPDEDEMAELDPQMLTNASSTLQKALAVKTNDELMVIYVSNLVRAIIAFDNLIENKIQNAKTQLKNKPDTESEKPEVDGKDEKTTADDIASAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DF54</accession>
    <name>C5DF54_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0D12320p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0D12320g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553247.1">
        <property type="nucleotide sequence ID" value="XM_002553201.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DF54"/>
    <dbReference type="FunCoup" id="C5DF54">
        <property type="interactions" value="72"/>
    </dbReference>
    <dbReference type="GeneID" id="8295487"/>
    <dbReference type="KEGG" id="lth:KLTH0D12320g"/>
    <dbReference type="eggNOG" id="KOG0255">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008455_11_3_1"/>
    <dbReference type="InParanoid" id="C5DF54"/>
    <dbReference type="OMA" id="LMPETHK"/>
    <dbReference type="OrthoDB" id="3936150at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015606">
        <property type="term" value="F:spermidine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000297">
        <property type="term" value="F:spermine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17323">
        <property type="entry name" value="MFS_Tpo1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF38">
        <property type="entry name" value="POLYAMINE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="114"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="144"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="203"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="274"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="313"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="410"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="438"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="473"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="513"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="8"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="560"/>
            <end position="580"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR22809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="588" mass="64471" checksum="4AAB4ACF7E961C2D" modified="2009-07-28" version="1">MDKSADCSGSMSNHSHISSSDPIDPRSLEWDGPMDPDNPHNWPTWKKWFATMTAALLCLVVTMGSSLYVNGVPRMVLEYGYSQTLALAGLTFYLLGLSTVVGAPLSEIFGRKPIYVASLPLSMLFTMGVALSGGKMRVVLPVRFFAGVFASPALSVASGTIVDIFDMDEVSVAMSLFCLAPFLGPVISPVIAGFATEKQGWRWATYIQLFAGGLIMPFVLLMPETHKTVILIKRAKKRSVNLIKPSAKDKKNFLKLTLTITVFRPIKMLLVEPTVLMFSIYVAFIFAVLFAFFEAYPVIFRGVYHMDGGISGLAFIGIGIGLWIGSVFYILYDRRFFFPAPPAGTPALKSADGKRTKPYRGKRDPVTGEIVPLKPEKLLDICKIGSIALPISLFWLGWTSKSSVHWMAPMAAGVPFGFGLILIFFSVIMYFSTCYPPLYVASALAANNLLRYVTSCVFPLFTIQMYEKMTIGWASSLFGFICVAMIPLPWIFERYGEGMRQKSTFGFNALEGEKGDEESCQSTSQKRFSHDDDPNFENGGITGPNVSNAKKEEAGVLQANGESSSVRSHALSSGESNISQPVLKGSFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFG1</accession>
    <name>C5DFG1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">tRNA-dihydrouridine synthase</fullName>
            <ecNumber evidence="16">1.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ordered locus">KLTH0D14806g</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>5,6-dihydrouridine(20a) in tRNA + NAD(+) = uridine(20a) in tRNA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53348"/>
            <dbReference type="Rhea" id="RHEA-COMP:13535"/>
            <dbReference type="Rhea" id="RHEA-COMP:13536"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.90"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="12">
            <dbReference type="Rhea" id="RHEA:53350"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>5,6-dihydrouridine(20a) in tRNA + NADP(+) = uridine(20a) in tRNA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53344"/>
            <dbReference type="Rhea" id="RHEA-COMP:13535"/>
            <dbReference type="Rhea" id="RHEA-COMP:13536"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.90"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="14">
            <dbReference type="Rhea" id="RHEA:53346"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>5,6-dihydrouridine(20b) in tRNA + NAD(+) = uridine(20b) in tRNA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53352"/>
            <dbReference type="Rhea" id="RHEA-COMP:13537"/>
            <dbReference type="Rhea" id="RHEA-COMP:13538"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.90"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="13">
            <dbReference type="Rhea" id="RHEA:53354"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>5,6-dihydrouridine(20b) in tRNA + NADP(+) = uridine(20b) in tRNA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53356"/>
            <dbReference type="Rhea" id="RHEA-COMP:13537"/>
            <dbReference type="Rhea" id="RHEA-COMP:13538"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.90"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="11">
            <dbReference type="Rhea" id="RHEA:53358"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 5,6-dihydrouridine in mRNA + NAD(+) = a uridine in mRNA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:69851"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:17789"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="9">
            <dbReference type="Rhea" id="RHEA:69853"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a 5,6-dihydrouridine in mRNA + NADP(+) = a uridine in mRNA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:69855"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:17789"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="10">
            <dbReference type="Rhea" id="RHEA:69857"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 16 18">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the Dus family. Dus4 subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the dus family.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.-" evidence="16"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553354.1">
        <property type="nucleotide sequence ID" value="XM_002553308.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFG1"/>
    <dbReference type="FunCoup" id="C5DFG1">
        <property type="interactions" value="160"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFG1"/>
    <dbReference type="GeneID" id="8295598"/>
    <dbReference type="KEGG" id="lth:KLTH0D14806g"/>
    <dbReference type="eggNOG" id="KOG2335">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013299_4_0_1"/>
    <dbReference type="InParanoid" id="C5DFG1"/>
    <dbReference type="OMA" id="QRPHHDI"/>
    <dbReference type="OrthoDB" id="9977870at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106414">
        <property type="term" value="F:mRNA dihydrouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102266">
        <property type="term" value="F:tRNA-dihydrouridine20a synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102267">
        <property type="term" value="F:tRNA-dihydrouridine20b synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02801">
        <property type="entry name" value="DUS_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000159">
        <property type="entry name" value="tRNA-dihydrouridine synthase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035587">
        <property type="entry name" value="DUS-like_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001269">
        <property type="entry name" value="DUS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018517">
        <property type="entry name" value="tRNA_hU_synthase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11082">
        <property type="entry name" value="TRNA-DIHYDROURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11082:SF31">
        <property type="entry name" value="TRNA-DIHYDROURIDINE(20A_20B) SYNTHASE [NAD(P)+]-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01207">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006621">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01136">
        <property type="entry name" value="UPF0034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 16" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3 16 18" id="KW-0288">FMN</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 16" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="DUS-like FMN-binding" evidence="19">
        <location>
            <begin position="34"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="17">
        <location>
            <position position="120"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="37"/>
            <end position="39"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00050434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00051779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00051932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00052996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00060741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR006621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR006621-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR006621-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CAR22916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="348" mass="39424" checksum="DC35FFFB08ED1857" modified="2009-07-28" version="1">MSSKYPQARILTKKNDPLHIIKTRKATHQRAVTIAGPMVRYSKLPFRQVCRHYNVDIVYTPMILAREYVRNCHARMADFSSNESDSPLIVQVGVNNVTDLLRFVEMVSPYCDGIGINCGCPIKEQVREGIGSALIYNPELLSSMVSAVKTKYGDKVRLETKIRIHDNWDQTVDLCNQLCDAGVDWITIHGRTRTTRSSEPVNLDAIKFITQKIKSRNVPVIANGDCFDMSDFQKILDYTGADGVMAVRGLLDNPALFGGFKACPWGALELFWHYTMEFGGLPHQLLLHHLHCMLGSMGLEKSLHNELMNIKDTSHLIDWFDYNFVLKRFGEQGFAEATDIPYKSHSKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFG8</accession>
    <name>C5DFG8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13 15">Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase</fullName>
            <ecNumber evidence="3 15">2.7.4.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">KLTH0D14982g</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Bifunctional inositol kinase that acts in concert with the IP6K kinases to synthesize the diphosphate group-containing inositol pyrophosphates diphosphoinositol pentakisphosphate, PP-InsP5, and bis-diphosphoinositol tetrakisphosphate, (PP)2-InsP4. PP-InsP5 and (PP)2-InsP4, also respectively called InsP7 and InsP8, may regulate a variety of cellular processes, including apoptosis, vesicle trafficking, cytoskeletal dynamics, and exocytosis. Phosphorylates inositol hexakisphosphate (InsP6).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>1D-myo-inositol hexakisphosphate + ATP = 1-diphospho-1D-myo-inositol 2,3,4,5,6-pentakisphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:37459"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58130"/>
            <dbReference type="ChEBI" id="CHEBI:74946"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.24"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:37460"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>5-diphospho-1D-myo-inositol 1,2,3,4,6-pentakisphosphate + ATP + H(+) = 1,5-bis(diphospho)-1D-myo-inositol 2,3,4,6-tetrakisphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:10276"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58628"/>
            <dbReference type="ChEBI" id="CHEBI:77983"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.24"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:10277"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Cytoplasm</location>
            <location evidence="1 15">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the histidine acid phosphatase family. VIP1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.24" evidence="3 15"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553361.1">
        <property type="nucleotide sequence ID" value="XM_002553315.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFG8"/>
    <dbReference type="FunCoup" id="C5DFG8">
        <property type="interactions" value="582"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFG8"/>
    <dbReference type="GeneID" id="8295605"/>
    <dbReference type="KEGG" id="lth:KLTH0D14982g"/>
    <dbReference type="eggNOG" id="KOG1057">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000914_3_1_1"/>
    <dbReference type="InParanoid" id="C5DFG8"/>
    <dbReference type="OMA" id="IQERWCC"/>
    <dbReference type="OrthoDB" id="18042at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033857">
        <property type="term" value="F:5-diphosphoinositol pentakisphosphate 1-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052723">
        <property type="term" value="F:inositol hexakisphosphate 1-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052843">
        <property type="term" value="F:inositol-1-diphosphate-2,3,4,5,6-pentakisphosphate diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006020">
        <property type="term" value="P:inositol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032958">
        <property type="term" value="P:inositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07061">
        <property type="entry name" value="HP_HAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000036">
        <property type="entry name" value="Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11950:FF:000002">
        <property type="entry name" value="Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013651">
        <property type="entry name" value="ATP-grasp_RimK-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000560">
        <property type="entry name" value="His_Pase_clade-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037446">
        <property type="entry name" value="His_Pase_VIP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040557">
        <property type="entry name" value="VIP1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12750">
        <property type="entry name" value="DIPHOSPHOINOSITOL PENTAKISPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12750:SF9">
        <property type="entry name" value="INOSITOL HEXAKISPHOSPHATE AND DIPHOSPHOINOSITOL-PENTAKISPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00328">
        <property type="entry name" value="His_Phos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18086">
        <property type="entry name" value="PPIP5K2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08443">
        <property type="entry name" value="RimK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ATP-grasp" evidence="17">
        <location>
            <begin position="304"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="136"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="39"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="109"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="143"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00071668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU365032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAR22923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1114" mass="125783" checksum="F71DB80EA243ABC0" modified="2009-07-28" version="1">MSDSKPKETGQGSMSPLLLNNKTKKAMESIAPILEGFSPKTSASENTSLKLPPPGSPHESHIDEDIGCDLNLDRTVSISSQLSSSSSRPGLSPTNSYDNIHPGGKLQRLKSNSNLGQSSTVASGNSALDFQHAAVESASNNDSGNETPTETPSSSANSSAGSSRKPSSNSGRPQLPKIGKIGVCAMDAKVLSKPCRHILNRLIQNGEFETIIFGDKVILDENNENWPTCDFLISFFSSGFPLSKAIDYVNLRKPFYINDLEMQKVLWDRRLCLKLLDVAKVPTPPRLEISRDGGPRVDPALRAKLLERGVEIKPVKEPAWKMVDDDTLEVKGRRMTKPFVEKPVDGEDHNIYIYYHSKNGGGGRRLFRKVGNKSSEFDPSLKSPRTDGSYIYEEFMDTDNFEDVKAYTVGEGFCHAETRKSPVVDGIVRRNTHGKEIRYVTELTPEEKEIARRVTKIFSQMICGFDLLRVNGNSYVIDVNGFSFVKDNSAYYDACAKILRETFIQAKKKMDLEKRNLPIIKEEKTQKWVFKGLVTVIRHADRTPKQKFKHSFRSPIFISLLKGHKEEVVIRNVNDLKVVLQALTIAQEENTEDPQKLKVLANALEKKMDLPGTKIQLKPVLNSEKEVEKVQFILKWGGEPTHSARYQATELGEQMRQDFDLLNKNILPNIKIFSSSERRVLASAQLWAMALFGADELGSDEISIRKDLLDDSNAAKDLMDNVKKQLKPLLREGKEAPRQFAWPAKMPEPYLVIKRVVELMNYHKHVMDYNFETKDVADMQRRWCCAEDPMLFKERWDKLFKEFVSVEKVDPAKISELYDTMKYDALHNRQFLEHIFASSGRAARANVDLCKDSLVDRYPINILAMNNFKIPDSSSQHGGTSSKGSSVGSLGWVLESSANTSRPSSQFDDPKFMQLRELYKLSKVLFDFICPQEYGIEDNEKLDIGLLTSLPLAKQILNDIDDMKKKDAPACVAYFTKESHIYTLLNIIYESGLPMRIARNALPELDYLSQINFELYESTDNSGQKAHSLRLKMSPGCHTQDPLEVQLDEKHYISCIPKISLTKHLDLDYVSQKLRNKFSRVILPKKFTPVNITSPNLTFRKGPLFESSSHPAQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFR7</accession>
    <name>C5DFR7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0D17358p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0D17358g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR23022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553460.1">
        <property type="nucleotide sequence ID" value="XM_002553414.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFR7"/>
    <dbReference type="FunCoup" id="C5DFR7">
        <property type="interactions" value="73"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFR7"/>
    <dbReference type="GeneID" id="8295710"/>
    <dbReference type="KEGG" id="lth:KLTH0D17358g"/>
    <dbReference type="eggNOG" id="KOG4313">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048013_0_1_1"/>
    <dbReference type="InParanoid" id="C5DFR7"/>
    <dbReference type="OMA" id="VPLQTMY"/>
    <dbReference type="OrthoDB" id="10261522at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044715">
        <property type="term" value="F:8-oxo-dGDP phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03676">
        <property type="entry name" value="NUDIX_Tnr3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000019">
        <property type="entry name" value="Thiamin pyrophosphokinase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031804">
        <property type="entry name" value="DUF4743"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13622">
        <property type="entry name" value="THIAMIN PYROPHOSPHOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13622:SF8">
        <property type="entry name" value="THIAMIN PYROPHOSPHOKINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15916">
        <property type="entry name" value="DUF4743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="1">
        <location>
            <begin position="152"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="339" mass="38513" checksum="8CB18606A26379DE" modified="2009-07-28" version="1">MTKVVKKGGKEVLLRDADDDFSYLSLVDEVDLLPEDYLKQEHFQHNVYHLQTGDRVSIGFVLKTVALKMAEVANSEFSSTFDMVEAESALIFKSKTYHGRNESLLTLARVLKEKKAFECLLGWRNEFYTIYVDRAPYVLVERALSGLLGVITYGVHVNGFVKNPSTGEIKFWIPRRSASKPTWPLKLDNIVAGGLGYPNGIFETVIKESLEEANLEQQLIEKHIKAVGAVSYFYFQGDIEGDKFQSESSLITGEVEFLFDLELPPDVVPCPNDGEVDSFQLMTLQQTIKALRNKEFKPNCGLIMLEFLMRHGYINSENEPNYGEIISRVHRRLPFPTLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFU7</accession>
    <name>C5DFU7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">KLTH0D18062p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0D18062g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR23052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553490.1">
        <property type="nucleotide sequence ID" value="XM_002553444.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFU7"/>
    <dbReference type="FunCoup" id="C5DFU7">
        <property type="interactions" value="354"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFU7"/>
    <dbReference type="GeneID" id="8295742"/>
    <dbReference type="KEGG" id="lth:KLTH0D18062g"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003977_1_0_1"/>
    <dbReference type="InParanoid" id="C5DFU7"/>
    <dbReference type="OMA" id="MAHQCLV"/>
    <dbReference type="OrthoDB" id="6077919at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002058">
        <property type="entry name" value="YML081W-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000145">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051059">
        <property type="entry name" value="VerF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40626">
        <property type="entry name" value="MIP31509P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40626:SF13">
        <property type="entry name" value="RESPIRATION FACTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="580"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="626"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="32"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="60"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="139"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="350"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="414"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="84"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="141"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="414"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR23052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1128" mass="128294" checksum="979DED28F2A3937B" modified="2009-07-28" version="1">MASQGAGNSSVAAKMPIPKKSRTIKTDRPRPFLCSICTRGFARQEHLKRHQRAHTNEKPFLCAFCGRCFARRDLVLRHQQKLHSSLTDTGSGASPNNGDAKNHLNERHIITISGNKQTMLPMLGSASDPSVAAISIPRQPTKVQESNPNPSQQMAPQVPQNQRQPVLSGQKQQKLSGTSITPHSHGPELEERQQKRKRHASFSASSSITYTQAKDASGIIEHDIPDIPHQVGFSTPQLSAQELVEKALESGFNLEVLPFPPPLELPEAAANPLMSQRHQSDDNLQRLPIHEPKRNHSASDVRSALSVDTPLLSDYLQMGSSAGGWGGFTNFYPTDSNLDFFSYNTPPDTMKPPHSSILTPVPDQSGKSKNGAERHGNSTDQHWLLEFINTHVDGNFKVDVDDFNEIGFPCTNSETTTSGSTPQVNNVTSTPFAKSEDTVEGPSSPKFSRNISENELSSVAINEAAAAGLKPNKKYHRKGDIPFFFKSRQIDLFKKIMENQGEFSSPLQDGDKNNIKSKKGSRLCFFNEELRNHILKTNDLTSDQFPTLKELNTYVNLYQDEFHRYFSFIHLHSIRPSAEIYPFLLSIAMIGALYGFHSSHAMLLFNICRFRKREYLEKTKGHHEETPLWIIQGMVLLIFVGIFNNDVSFTLIMSTQIRSLIELIKIKQLNLPLEALIKPPIESDHILDYQDDPQLLEQKRREYNTPEQVERNYHYFIHAQSRIRTCHTILLISNLFNSLVGLDCCFHSIDVKCGIPCYRDELYFCETPQEWAKLLKSYHIILDSKFSLIDLSNGGETYKNCLIYLTNGYQFFYENKKVSFKTLLSLLISIHEKIFIEGYNLRHELNDQIVDVKWRMNSRPIIESLLKYWEALYIKNGGVIIANEGNIAFINSNPSLRLIIPLLNFAKIRKCLLLTNVMKKIWFKDWEGMNECLEDFRDWESLREATDYALNIVKFWVDTVFIVKDAEKTSIRTPIFSITCIFSSILIVAEYLKRVETWARCFRNSTTTEFLKAVDRTLWLKSETILKKVEEHLLPKGYNTQSYAEFLRLQANGALDVEVLDDDLARKAMSPNTDIKETVEVILKARISSRSLYLGVRILGDAPIWPIALLFAHALQSRAIFNLAYRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFV4</accession>
    <name>C5DFV4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0D00132g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. Yeast subtelomeric Y' repeat subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552734.1">
        <property type="nucleotide sequence ID" value="XM_002552688.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFV4"/>
    <dbReference type="FunCoup" id="C5DFV4">
        <property type="interactions" value="274"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFV4"/>
    <dbReference type="GeneID" id="8294948"/>
    <dbReference type="KEGG" id="lth:KLTH0D00132g"/>
    <dbReference type="eggNOG" id="ENOG502QWCT">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003044_2_0_1"/>
    <dbReference type="InParanoid" id="C5DFV4"/>
    <dbReference type="OMA" id="GLANIGH"/>
    <dbReference type="OrthoDB" id="4070089at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18785">
        <property type="entry name" value="SF2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037240">
        <property type="entry name" value="ORC1-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021646">
        <property type="entry name" value="Sir1_ORC-binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF1">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11603">
        <property type="entry name" value="Sir1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144005">
        <property type="entry name" value="ORC1-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="807"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="1028"/>
            <end position="1181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1211"/>
            <end position="1258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1213"/>
            <end position="1229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR22296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1258" mass="144548" checksum="7220AF769A6A3D1C" modified="2009-07-28" version="1">MEKISDRFAAIHGFLIKTNRDGTKRITCPNISSLKPTKKEVQRILELKLQNLAVYLGMKEDLIYVDCPLLFETVTSEQRYFYSTEDGMKAVRSGRTNSANTRRSIEYRVLPITLPNKTRKYLCQTGKKLPEMPLEHEIATPRQESEAETATVPQASEDLDVFHTLHCDKLVFTNRDRRLFRSFAMDRTLYEVESVAILKHCHREAISKTTKTNSFGEPLVTKYKLETLEIHGKHAGVLLFLIANLHQLSLGQQVERPTVDSELLLDRIGELHNTEAKAAHLQQLLYIVLKEERYMDLANLFTRLLLFRLSAHFDIRKDLEVEEMETVRERMSATGVWIKCVIISEKSEDQLNIFQEESHNIWSKFTTLYHKVAKESDQTMTMNINRVPPDCIRVYGKTFGREYIKSFYSDMQYEFDQQWSKLDRVLTMTPVESTGKMLLENYRIPWAGHGYRANFSLKDMFPQWIHDQVSPSMRQPLTQLDKREVLGAIDKMTVALMWMIWLAAGVPYTFSELQVLACGGDQQNIYVDSERRCVQIITRSFKKGNLKEDFKWLDKRTSYYLVYYVTVLRKTQISVLGENYEAFEPPKEGEVDLEGIWEHIEGKCNMSKKEASALIMDQYLFLDAKLGQLMSRRRFLKPSEEHPNYVASPERLSFKQLKFGRMGLLRQYLASSLNDSKPVKTARIDLAMCRFAASSARDYMDELGDSEDVDDPDAMGDLEVLEESMWQCEQSERFMTRTWHEEIAKQWIRWVGLDEEEEIHHPLTYSVGEIVLQKEALCPDDLVHAGRELYGKSFTFRNSDQYLACVNAYLSNKKIIAVQAPPGLGKTLVFQLPLMCYTMRKLSLVSFVFVPFVGLLAGTMCRLEASGNLRVGSVEKLLNETRCKENSLLDVYVGTFNDAAAEDFIKMINFWSLLYPEKKLGLLAFDEFQNLYDEKSHSEVSFPSLKRVQFGRFRRIILTSATCEQGSAQGALQELGFRGREGTNFTWERSQAGRSVDVPIFLRNYVQEPAVMHVYKHALRMRGNGVGQVKQFLRSLFAYDPDAKAALVVGENVATTELFTEVSQEFSAAYVHSDLTTEEKLKRVQSFIKDKSLQLLIGTKQVVEGLDIEGLRMVILLDYYPRVAEYIQCVGRVRGSGTCIALWQDPPPQVENDQPRVDYDKCFTSQICKYYELPQRVHAGCCGSFESVTEEAKSLFHRVVLEEEVNDVCSQKTTRDEDSEQGRERKIARTDVIASADDSNEVSGVNKRPQDDTAITKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>C5DG12</accession>
    <name>C5DG12_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0D01562p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0D01562g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552792.1">
        <property type="nucleotide sequence ID" value="XM_002552746.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DG12"/>
    <dbReference type="FunCoup" id="C5DG12">
        <property type="interactions" value="106"/>
    </dbReference>
    <dbReference type="GeneID" id="8295012"/>
    <dbReference type="KEGG" id="lth:KLTH0D01562g"/>
    <dbReference type="eggNOG" id="ENOG502S3NH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071381_0_0_1"/>
    <dbReference type="InParanoid" id="C5DG12"/>
    <dbReference type="OMA" id="DVIMINF"/>
    <dbReference type="OrthoDB" id="4069549at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR22354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="311" mass="33959" checksum="B7189E6F7D1229CA" modified="2009-07-28" version="1">MSFHHQFTLASAKNQLIPQQLTNNVFFGPLNALSQREFIQENRIRFFVAVGIATKRVAQYCRDMPARDCLVVNFDSEFSPQVSLSATELELVSQYSQQHSTSLSQLAAGLAGDAATGCTADSRLTPQPELDQQLHNSPAQYTCNIVTSQGAQKFASFNDLLTLFKLSGSGNVLVFSSNGNDEDLVALLSSHVVITNSSASLLEAFQYVKSLRPSINQCQPDSVFWCQGLMEYLEGSRSYNNPFYDNGMSPASPLSTTGVTSNAFVTKRRNSWSSDCEEECPNSATGTISVSGTPKTRRIAAIRADNMALPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DG33</accession>
    <name>C5DG33_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">KLTH0D02046p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0D02046g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">SNC1 and SNC2 are vesicle-targeting proteins essential for normal secretory traffic between the Golgi and the plasma membrane. They may also be involved in vesicle fusion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endomembrane system</location>
            <topology evidence="7">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the synaptobrevin family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552813.1">
        <property type="nucleotide sequence ID" value="XM_002552767.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DG33"/>
    <dbReference type="FunCoup" id="C5DG33">
        <property type="interactions" value="351"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DG33"/>
    <dbReference type="GeneID" id="8295033"/>
    <dbReference type="KEGG" id="lth:KLTH0D02046g"/>
    <dbReference type="eggNOG" id="KOG0860">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064620_2_1_1"/>
    <dbReference type="InParanoid" id="C5DG33"/>
    <dbReference type="OMA" id="VRKKMWW"/>
    <dbReference type="OrthoDB" id="190375at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030658">
        <property type="term" value="C:transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd15874">
        <property type="entry name" value="R-SNARE_Snc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000063">
        <property type="entry name" value="Synaptobrevin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001388">
        <property type="entry name" value="Synaptobrevin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016444">
        <property type="entry name" value="Synaptobrevin/VAMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45701">
        <property type="entry name" value="SYNAPTOBREVIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005409">
        <property type="entry name" value="Synaptobrevin_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00219">
        <property type="entry name" value="SYNAPTOBREVN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00417">
        <property type="entry name" value="SYNAPTOBREVIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="85"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="11">
        <location>
            <begin position="21"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00058407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR22375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="109" mass="12212" checksum="9B59FFC924B7F704" modified="2009-07-28" version="1">MSSSVPYDPYIPAEESQSQTKTAALKAEIDDTVGIMRDNINKVAERGERLTSIEDKADNLAVSAQGFKRGANRVRKQMWWKDLKMRLCLVLAVIILLVVIIVPIVVHFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>C5DG48</accession>
    <name>C5DG48_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mediator of RNA polymerase II transcription subunit 15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0D02376g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Mediator complex subunit 15 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552828.1">
        <property type="nucleotide sequence ID" value="XM_002552782.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DG48"/>
    <dbReference type="FunCoup" id="C5DG48">
        <property type="interactions" value="272"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DG48"/>
    <dbReference type="GeneID" id="8295048"/>
    <dbReference type="KEGG" id="lth:KLTH0D02376g"/>
    <dbReference type="eggNOG" id="ENOG502QVXD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009962_0_0_1"/>
    <dbReference type="InParanoid" id="C5DG48"/>
    <dbReference type="OMA" id="RYQKYYE"/>
    <dbReference type="OrthoDB" id="1938591at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12191">
        <property type="entry name" value="gal11_coact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.20">
        <property type="entry name" value="Coactivator CBP, KIX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033789">
        <property type="entry name" value="Gal11_coact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036529">
        <property type="entry name" value="KIX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036546">
        <property type="entry name" value="MED15_KIX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008626">
        <property type="entry name" value="Mediator_Med15_fun"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18535">
        <property type="entry name" value="Gal11_ABD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16987">
        <property type="entry name" value="KIX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05397">
        <property type="entry name" value="Med15_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mediator complex subunit 15 KIX" evidence="6">
        <location>
            <begin position="3"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="Gal11 coactivator" evidence="7">
        <location>
            <begin position="161"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="233"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="458"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="704"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="840"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1007"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="105"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="233"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="271"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="326"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="470"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="708"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="797"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="840"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1025"/>
            <end position="1034"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF16987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF18535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR22390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1086" mass="121773" checksum="8FBAF993275FEB24" modified="2009-07-28" version="1">MEADWRTTLSNQERSKYITELAQILAQISQVNGGDRGNFNLEKLKKTAEQFEKSLYASSSSKELYLDSMRKRIAAMDTAKKKAIANIQHNAANVAAHQQSQQQAAVAAAQSAQQQRASQFPPSNMNSQMFFNQQAQVRQQAAQQFRNGVGSNMPVNSAPTRPQLTPQQQQLINEMKGAEIPRELLQRIPNLPPGVNTWQKVTELAQQKRLGPKDLQIAKQVYKMHQQIVFKSKMQQASANNRGIPQQRAMPQGPNMAQMPQPRSVVNQPLQQQQHQQQQQQQQHQQHQQQQQQQQQHQQHQQQQQQQQQHQHQQHQQQQQQQQQQRQTGQVPQPSGQEMPNVLNRLNQIFTPAEQKALYENGKKLIEDLQRTNRLPTVLTPQQQAIYIKKYINQMALKKLQAARMNQMGTTNPQQQMAPAPAPQGQSVPAMSQFTSNVAPSNANLAGMAQRRAYERANAGGASDVNSVGSSTQAQLSSGSSQQAPPAQQQPQPQPKFSLPRPTEQDLMVLRRISAEIQKSHLRLSNITNQISQEQKQAIRNKLQLNRQLFTNVDSFIPTLYMITRNEENVRQLLQIRMLAKEITEHATRGVFIVPPEVVDKVIFRYQKYYEFIKDQVLRRHQQIIAARQQQQQQQQAPSTTIDPAFNQQRIQGSFQQIQQQMQMRQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQKIQPSPQLNNWTRLNADGRSTSGADGDNAVAPQTAPEHVRRSSSGMDFLNSPEFMNARSSPQQPGLSPAKKQAQMRKKMAMKQVPGQGTPGSTVPTSNNTPVPVSGPVPAVASRTGTPQVGTAGISSISPMVNRATGLNQSPSPRSHPNGTLPPPADYPYKEDEENLKKMAIRKTEIIARFKCRQEILGKSSVDLFLCSLGDCLGLPSASVDLISPIPSEIVEHVNGGCKGKNNGAPGQRVKGQDLTDVSIVNNSIVFADSNSPEAKAEPYVIGLNDVASVFRDVYGSTDLSSFSFETKTFSGEGGAKKRKAGDLDNSPDASPASSAIMSESKKLKIDSPEDMYFTAPGEDTKQLLTKSGAMKFSPENKIWDWSFWETS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGB5</accession>
    <name>C5DGB5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0D03960p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0D03960g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase PH family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552895.1">
        <property type="nucleotide sequence ID" value="XM_002552849.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGB5"/>
    <dbReference type="FunCoup" id="C5DGB5">
        <property type="interactions" value="181"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGB5"/>
    <dbReference type="GeneID" id="8295120"/>
    <dbReference type="KEGG" id="lth:KLTH0D03960g"/>
    <dbReference type="eggNOG" id="KOG1068">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078569_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGB5"/>
    <dbReference type="OMA" id="ILPTCIN"/>
    <dbReference type="OrthoDB" id="2504340at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071028">
        <property type="term" value="P:nuclear mRNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071051">
        <property type="term" value="P:poly(A)-dependent snoRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050080">
        <property type="entry name" value="RNase_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953:SF2">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT MTR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0271">Exosome</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="9">
        <location>
            <begin position="42"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR22457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="243" mass="26741" checksum="1D602804E1AABD05" modified="2009-07-28" version="1">MNITDRRRILGPSNAKPLVFERLESSEQAAQPPQEKNNENESMYIKNGLVSNANGSSYLELKRGESSEQCTILLSSVYGPRPSRGAFSSKASLSVQFKEVTIEKIPSGELKEICNFLSNIFSAVINLERYPKSGIDIFLSLIKNPITSDEANLEPIIAACVNGITLALADAGIEIFDTVSAGTYKRNVVAFSKNGTEVVGLWKDKEDDTANDILSVIENCRGNYLQNRKGIIEYLIRSKNADI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGG3</accession>
    <name>C5DGG3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Apurinic-apyrimidinic endonuclease 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0D05104g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AP endonuclease 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552943.1">
        <property type="nucleotide sequence ID" value="XM_002552897.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGG3"/>
    <dbReference type="FunCoup" id="C5DGG3">
        <property type="interactions" value="65"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGG3"/>
    <dbReference type="GeneID" id="8295171"/>
    <dbReference type="KEGG" id="lth:KLTH0D05104g"/>
    <dbReference type="eggNOG" id="KOG3997">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025885_1_0_1"/>
    <dbReference type="InParanoid" id="C5DGG3"/>
    <dbReference type="OMA" id="HPGSHLR"/>
    <dbReference type="OrthoDB" id="7663182at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00019">
        <property type="entry name" value="AP2Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.150:FF:000001">
        <property type="entry name" value="Probable endonuclease 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.150">
        <property type="entry name" value="Divalent-metal-dependent TIM barrel enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00152">
        <property type="entry name" value="Nfo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001719">
        <property type="entry name" value="AP_endonuc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018246">
        <property type="entry name" value="AP_endonuc_F2_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00587">
        <property type="entry name" value="nfo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002199">
        <property type="entry name" value="PRK01060.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21445:SF0">
        <property type="entry name" value="APURINIC-APYRIMIDINIC ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21445">
        <property type="entry name" value="ENDONUCLEASE IV ENDODEOXYRIBONUCLEASE IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00518">
        <property type="entry name" value="AP2Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="Xylose isomerase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00729">
        <property type="entry name" value="AP_NUCLEASE_F2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00730">
        <property type="entry name" value="AP_NUCLEASE_F2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00731">
        <property type="entry name" value="AP_NUCLEASE_F2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51432">
        <property type="entry name" value="AP_NUCLEASE_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Xylose isomerase-like TIM barrel" evidence="10">
        <location>
            <begin position="77"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="348"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="348"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="372"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="387"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR22505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="405" mass="45333" checksum="C867FBAEEA70FFB0" modified="2009-07-28" version="1">MRVSPASYHAFHPASNMYVKEFDSKKVVMLSKKNVVTHSTYSLTETVMSQFSRCSTSKYKFGAHISTAGGISNSVTNAYNIGCNAFAMFLKSPRKWVSPDYTQEEIDKFKQKCKELGYNPLTDVLPHGQYFINLANPEPEKAEKSYASFIDDLKRCEQLGVGLYNFHPGSSLKGNHAKQLRQLASYINKAIAETKFVKIVLENMAGTGSLVGSDLQDLKDVIDMVENKDRVGVCVDTCHTFAAGYDISSKDSYDKFWQTFDKTVGFKYLSAIHLNDSKAPLAANRDLHEKLGQGFLGLEVFKLVANSEFLRGIPVILETPHTKDEGYGHEIKLLEWLEGLDEAKDQPEFESKSKDLLSEGAKSRNEQLAKFDKKKTKATKVSKKRANNGTDISQQLSSKRAKKET</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGL3</accession>
    <name>C5DGL3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0D06248p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0D06248g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552993.1">
        <property type="nucleotide sequence ID" value="XM_002552947.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGL3"/>
    <dbReference type="FunCoup" id="C5DGL3">
        <property type="interactions" value="71"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGL3"/>
    <dbReference type="GeneID" id="8295223"/>
    <dbReference type="KEGG" id="lth:KLTH0D06248g"/>
    <dbReference type="eggNOG" id="ENOG502QPQM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030747_2_0_1"/>
    <dbReference type="InParanoid" id="C5DGL3"/>
    <dbReference type="OMA" id="WSIYDAQ"/>
    <dbReference type="OrthoDB" id="5784at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070916">
        <property type="term" value="C:inositol phosphoceramide synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006676">
        <property type="term" value="P:mannosyl diphosphorylinositol ceramide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03386">
        <property type="entry name" value="PAP2_Aur1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026841">
        <property type="entry name" value="Aur1/Ipt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052185">
        <property type="entry name" value="IPC_Synthase-Related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31310:SF11">
        <property type="entry name" value="INOSITOL PHOSPHORYLCERAMIDE SYNTHASE CATALYTIC SUBUNIT AUR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14378">
        <property type="entry name" value="PAP2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="45"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="74"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="147"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="176"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="263"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="7">
        <location>
            <begin position="177"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="374"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="386"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR22555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="414" mass="46412" checksum="29B78854045975CB" modified="2009-07-28" version="1">MSEIFKRLFMSHRPANSKVSQLETSLNPTITLKKLRSYTPSLGDLCHYAFLGSVLLFVLIVYPAALILKLSVYVLFGVLFLLPATSQFFFNALPIFTWLALYFTSSTFEAKHRPPITVQVLPAIETILYGDNLSDILAASLNKPLDIFAWLPYGIFHFGAPFVVAIILFLCGPPTLLRGYAFAFGYMNLAGVIMQNLLPAAPPWYKIQYGLTPANYKMKGSPGGLKRIDEYLGISVYTEGFSNSAVIFGALPSLHSGCATMEALFFSYVFPKLKPLFIFYVCWLWWSTMYLTHHYFVDLMAGSVLSYTIFQYTKYFHLPLPEPSVYSRWSYADIKKFSVWQADPLNGDGGDLENLAVPLAVDTDFELNVMENSEPPSIFDQDRSVSRSSATSNTSADVQDDFVSAARPGKQRID</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGP7</accession>
    <name>C5DGP7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0D07040p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0D07040g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553027.1">
        <property type="nucleotide sequence ID" value="XM_002552981.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGP7"/>
    <dbReference type="FunCoup" id="C5DGP7">
        <property type="interactions" value="275"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGP7"/>
    <dbReference type="GeneID" id="8295258"/>
    <dbReference type="KEGG" id="lth:KLTH0D07040g"/>
    <dbReference type="eggNOG" id="KOG4299">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016350_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGP7"/>
    <dbReference type="OMA" id="CCDPPIE"/>
    <dbReference type="OrthoDB" id="5876363at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032221">
        <property type="term" value="C:Rpd3S complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15535">
        <property type="entry name" value="PHD1_Rco1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052819">
        <property type="entry name" value="Chromatin_regulatory_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47636">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATORY PROTEIN RCO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47636:SF1">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATORY PROTEIN RCO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="6">
        <location>
            <begin position="284"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="227"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="54"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="64"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="146"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR22589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="674" mass="75000" checksum="377D1692835D6F87" modified="2009-07-28" version="1">MSDPARHPPERSGSPGEPATRARRTISLNVDYNLKKRRIIPSEDARPRKNRPVGRPPGRPPGRPAGSKTASSTPSSTPSSSAASSLSPQDENHRAPQSVIQHDATGAVQFHEPESVDPVNGMTGLPLSAGPPEKVKKESLWSYRKGAGASASASTNASVNAATPAPSESSASVSAPASASASADVETPASSEDAYRARLQTQESEQRTRPSSLRAIATPKDIYRTKRQYRKRSAEPRARHFPTHTKIKASTTKENKLFGQNSITNQKALAPPLASSAEAAVENDDFCASCRQPGIFLCCDTCPKSFHFACCNPPLDPDNLPEGDWSCAECQFRIRCPNKAAAHRLEKDYLAQLERSKGITLFGKLLFRLEFTNPKQYLPPHPIRDAFADVRTGSHGEYADNSMREPLNEKQIFGSGYGQSLTKMDQYNPELHVDPESGELLVCFKCRKSKMGTLDHPEDERLITRCDYCNTPWHLDCVPHVPRASFKNLGSKWMCPLHADSSIPHQERRLARHQRHFTPANRYRVPNDGDIDVQLDEISVPVSKDALKEDKINKELAILQLPESSIKIDFVDKIYRAKSALMDKHFRQQQALIDNTMHSKPSLLSDARPLLYFSLQDNSQLRKLWDFKELCRVATKELEVPELDSAEISELQTLRKLLESKSKEEVMEFLGIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGW7</accession>
    <name>C5DGW7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Endoplasmic reticulum transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0D08866g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">May play a role in anterograde transport of membrane proteins from the endoplasmic reticulum to the Golgi.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Endoplasmic reticulum membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the BCAP29/BCAP31 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553097.1">
        <property type="nucleotide sequence ID" value="XM_002553051.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGW7"/>
    <dbReference type="FunCoup" id="C5DGW7">
        <property type="interactions" value="151"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGW7"/>
    <dbReference type="GeneID" id="8295333"/>
    <dbReference type="KEGG" id="lth:KLTH0D08866g"/>
    <dbReference type="eggNOG" id="KOG1962">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087648_1_0_1"/>
    <dbReference type="InParanoid" id="C5DGW7"/>
    <dbReference type="OMA" id="QRNMYIS"/>
    <dbReference type="OrthoDB" id="435607at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070973">
        <property type="term" value="P:protein localization to endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008417">
        <property type="entry name" value="BAP29/BAP31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040463">
        <property type="entry name" value="BAP29/BAP31_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12701">
        <property type="entry name" value="BCR-ASSOCIATED PROTEIN, BAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12701:SF19">
        <property type="entry name" value="ENDOPLASMIC RETICULUM TRANSMEMBRANE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05529">
        <property type="entry name" value="Bap31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="49"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="BAP29/BAP31 transmembrane" evidence="7">
        <location>
            <begin position="1"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="147"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU367026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR22659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="207" mass="23021" checksum="2A2105857B889483" modified="2009-07-28" version="1">MSVYLSVLFGALTLEMACLFVLVLPLHYKIRKGLVSTYDRFMSYTQVKTVIWITAGLVGLLFVDSWKRAQVSVFLHHHQGVGGPSANPDGAGAITPVQALASRAYNQRNTYISGFILYFCFCIPTVISVVRRLVKYETLLRDKDSQHEGQNEAETKAEAEVAKLKKELSEKKASIKALAKQKANLEAHFDSSAEAQKNADPKLKKDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGX0</accession>
    <name>C5DGX0_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST2</fullName>
            <shortName evidence="8">Oligosaccharyl transferase subunit OST2</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0D08954g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the oligosaccharyltransferase (OST) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Endoplasmic reticulum membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the DAD/OST2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553100.1">
        <property type="nucleotide sequence ID" value="XM_002553054.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGX0"/>
    <dbReference type="FunCoup" id="C5DGX0">
        <property type="interactions" value="758"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGX0"/>
    <dbReference type="GeneID" id="8295337"/>
    <dbReference type="KEGG" id="lth:KLTH0D08954g"/>
    <dbReference type="eggNOG" id="KOG1746">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111220_2_1_1"/>
    <dbReference type="InParanoid" id="C5DGX0"/>
    <dbReference type="OMA" id="YCCSVGT"/>
    <dbReference type="OrthoDB" id="445566at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008250">
        <property type="term" value="C:oligosaccharyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003038">
        <property type="entry name" value="DAD/Ost2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10705">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT DAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10705:SF0">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT DAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02109">
        <property type="entry name" value="DAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005588">
        <property type="entry name" value="DAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="72"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR22662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="126" mass="13684" checksum="3B10322C5CF19E20" modified="2009-07-28" version="1">MVPKTTPKKADVASSASKFSDTISASYKAYCKQVSGDRQLQLIDTFCAFLVAVGVIQFLFVCAAKDSFPLNAFLAGFSACVGQFVLLVSLRMQVVNSFMGISPQRAFGEFVLASLVLHFLCLHFVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGY0</accession>
    <name>C5DGY0_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">phosphoinositide 5-phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.36</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0D09174g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the synaptojanin family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the central section; belongs to the inositol 1,4,5-trisphosphate 5-phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.36" evidence="4"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553110.1">
        <property type="nucleotide sequence ID" value="XM_002553064.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGY0"/>
    <dbReference type="FunCoup" id="C5DGY0">
        <property type="interactions" value="313"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGY0"/>
    <dbReference type="GeneID" id="8295347"/>
    <dbReference type="KEGG" id="lth:KLTH0D09174g"/>
    <dbReference type="eggNOG" id="KOG0566">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003016_2_0_1"/>
    <dbReference type="InParanoid" id="C5DGY0"/>
    <dbReference type="OMA" id="HPCHELR"/>
    <dbReference type="OrthoDB" id="405996at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043813">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004439">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09090">
        <property type="entry name" value="INPP5c_ScInp51p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000029">
        <property type="entry name" value="Inositol polyphosphate 5-phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046985">
        <property type="entry name" value="IP5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000300">
        <property type="entry name" value="IPPc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002013">
        <property type="entry name" value="SAC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200">
        <property type="entry name" value="INOSITOL 5-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200:SF257">
        <property type="entry name" value="PHOSPHOINOSITIDE 5-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22669">
        <property type="entry name" value="Exo_endo_phos2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02383">
        <property type="entry name" value="Syja_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00128">
        <property type="entry name" value="IPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50275">
        <property type="entry name" value="SAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SAC" evidence="11">
        <location>
            <begin position="143"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="937"/>
            <end position="1117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="937"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="987"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1035"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1054"/>
            <end position="1069"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR22672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1117" mass="125036" checksum="F6FF930EA1030FF6" modified="2009-07-28" version="1">MQILVQRGAERKVALVSTAYALVFKAVNDGQNSTKPLCAIELTPKSQIGSSYQKMSSHEVHGFIGLIELEGFIFICTITGKTKVAQPIPGETVNKIYAVDFFCLNDSQWDFVELDSNGYPVTSEDDYRGEQSPSYPGHPCHELRKLLSNGSFYYSSNFDLTSILQKRGMGAHSLSFDNYQEEYMWNSFLMKEVITFRNRLDDLGRQVLDDEGFLTTVIRGFAETFVTYIKQLKVSLTVISKQSWKRAGTRYNARGVDDEGNVANFVETELLMFSKEYCYAFTQIRGSIPIFWEQDTALINPKVQITRSVEATQPVFDDHFTRLIDKYGPVHVVNLLSTKSSEVELSARYKQHVARSKLHTLNKDIFFTDFDFHKETSQEGFAAAARIKPLITQSLLESGYFSYDVRERKVLSEQHGIFRTNCLDCLDRTNLIQQFISRYAFALFLNDFHLMSTARGTAFTDSDLFIKHNTLWADHGDQISQIYTGTNALKSSFSRKGKMSFAGVLSDATKSVSRMYINNFMDKNKQANIDTLLGRLSHQQAVSLYDPINEYVTTQLTKSSDQFTSSSSINIFVGTYNVNGLTRRVDISKWLFPIGDKFKPDVVVLGMQEIIELSAGSILNADYSKSPFWQNIVNECLNQYNDKYLLLRAEQMSSLLILFFVKSDKINNVKQVEGSSKKTGLGGMTGNKGAVSIRFDYGSTSFCFVNAHLAAGVSNVDERRHDYESITKGICFTRSRQISHHDAIFWLGDLNYRLSLPNEEVRREISDQKDNFPESLLMHDQLTQEINAGVVFKDFKEPTVKFPPTYKYDHGTNNYDTSEKARTPSWTDRIVYKGNNLRPLAYSDSQLIASDHKPVYAAYRAQVNFVDETVKMDLTKKLYMQYKESHPEEYNNTTVALVDIDYEKKNGHGAKSSSTQLFAESDISLVDLDENRADVGSITRSSSISSSSEMSSLNSGTLNPVRKEAPSRSTTSLGRARVPPPPPPAPRQNSMASQFSSASNLQAKAEPVNAETTSTASVVAPPPPPARKTTFPPGYSSSILTPKNASAAGSKLTSPEPSVSSAPVPDASSTQNSKTDEHAAVSEAPINKPSRIPPPRPAKKPTLDNLNMDSWKPLTPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DH50</accession>
    <name>C5DH50_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0E01364p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0E01364g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553548.1">
        <property type="nucleotide sequence ID" value="XM_002553502.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DH50"/>
    <dbReference type="FunCoup" id="C5DH50">
        <property type="interactions" value="21"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DH50"/>
    <dbReference type="GeneID" id="8291686"/>
    <dbReference type="KEGG" id="lth:KLTH0E01364g"/>
    <dbReference type="eggNOG" id="KOG2399">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004979_2_1_1"/>
    <dbReference type="InParanoid" id="C5DH50"/>
    <dbReference type="OMA" id="CASDYLC"/>
    <dbReference type="OrthoDB" id="407410at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051359">
        <property type="entry name" value="CaCA_antiporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12266:SF0">
        <property type="entry name" value="MITOCHONDRIAL SODIUM_CALCIUM EXCHANGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12266">
        <property type="entry name" value="NA+/CA2+ K+ INDEPENDENT EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="26"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="69"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="105"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="139"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="393"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="421"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="473"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="557"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="614"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="647"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="8">
        <location>
            <begin position="34"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="8">
        <location>
            <begin position="481"/>
            <end position="663"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR23111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="672" mass="74021" checksum="D10F5B499E300251" modified="2009-07-28" version="1">MHEKAENWLFRLVHPSLLYLDSKLNVQFVISSLLYLSLCFVLLGICASDYLCPAVSNISNGRKGSAQKGLLAAILLSWCNSSPDLFTNFMSWTSSNAAALSVGEVLGSCGFIMCVVQGAVFIVMSKARVGLEPEQRQHVTRDLGFILVAMLMMLYVCLQNEVTLLNCVFMMGVYAAYIASKAGVRYSKPTSDDANHTDWNSPEEAFPEMTAGAFSSGADSGHKFNILSAMDYSALYEMMERSTSSPDDAITLQTLRSDIPGTTFVSPRPITEPSASRLPFLDNVASPVQSSPSTFQPFHDSDDISEAGQALLAEPIKYSKIAIKRLQRLRKGAFFLLAPQLLGFGCKSLSAKALALATTPFTVLLRFSCPQYYEILQFDRTPSNLIELERAQLMLLLVQALLAPSSSLVLLWVLAGILLRWYYLLAAGITAIALLVSVAFLQLAAKKYNRFSLNEDHSSDDRKLKRLLNAEKAIIILFNSFGILNSILWISTLANALVEVMEVYQRFTSVSEAVLGLTIFSWGNSISDLMSNVAMCQLYRKNEVEDTEETSRHASRSFFISLSACFGGILLNSLIGIGLSGFVAMLINTEGTNEKNSWILRSVSLDSSGVDFKFMVSVSFIIIQNLFLLSSFCGVRTFDSLLEDKRKLVGILMCSWWGAATLVNVLIESFVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHA0</accession>
    <name>C5DHA0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0E02552p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0E02552g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553598.1">
        <property type="nucleotide sequence ID" value="XM_002553552.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHA0"/>
    <dbReference type="FunCoup" id="C5DHA0">
        <property type="interactions" value="57"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHA0"/>
    <dbReference type="GeneID" id="8291740"/>
    <dbReference type="KEGG" id="lth:KLTH0E02552g"/>
    <dbReference type="eggNOG" id="KOG0252">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_46_12_1"/>
    <dbReference type="InParanoid" id="C5DHA0"/>
    <dbReference type="OMA" id="RGPIFIL"/>
    <dbReference type="OrthoDB" id="2153661at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046943">
        <property type="term" value="F:carboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001406">
        <property type="term" value="F:glycerophosphodiester transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17364">
        <property type="entry name" value="MFS_PhT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000140">
        <property type="entry name" value="Putative MFS phospholipid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508:SF10">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="27"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="167"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="202"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="257"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="294"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="320"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="346"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="415"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="32"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR23161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="500" mass="55147" checksum="B4454E8B7C706073" modified="2009-07-28" version="1">MSETNRYVTYDADARKMRVNTSREKRLYNTFMIVAAGFALISDGYQNNVMSMLNKVFPLEYGKHAYNGDVSTRVSNASLVGTILGQVTIGVACDYMGRKWAILVATLMLVGGSALCAASHGKTVSGMFWMLTVMRGVTGFGIGAEYPCCSVSANEAANEHSEKKRGGIMVMVTNLPLSLGGPFANIIFLIVFSICGKNHLNALWRVMFALGCFWPLSVFYFRWKTSTNTLFQKGQFKSQIPYWLTIKYTWKRLLGRCFVWFCYDFVAFSNSLFSGTIISLVIKNGNDLKTIAEWNLLLGIIAVPGCFVGAWLCDRIGRKYTLMFGFSGYLVFGLIIGCAWDKISKITALFIIFYGLMNGLANAGPGDMLGLVSSESFPTAVRGTCYGLSAAIGKVGAVVGTECFTPIKDRLGIRWVFIIAALCGVAGIIATWLLIPHLLESDLMEQDINFHNFLVKHGWSGTMGFDTEDRLENKNSDADETADSLNKDLNPEIITREISV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHA8</accession>
    <name>C5DHA8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">KLTH0E02772p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0E02772g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553606.1">
        <property type="nucleotide sequence ID" value="XM_002553560.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHA8"/>
    <dbReference type="FunCoup" id="C5DHA8">
        <property type="interactions" value="1463"/>
    </dbReference>
    <dbReference type="GeneID" id="8291750"/>
    <dbReference type="KEGG" id="lth:KLTH0E02772g"/>
    <dbReference type="eggNOG" id="KOG0254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_30_1_1"/>
    <dbReference type="InParanoid" id="C5DHA8"/>
    <dbReference type="OMA" id="CLVFMYF"/>
    <dbReference type="OrthoDB" id="5141738at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055056">
        <property type="term" value="F:D-glucose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17356">
        <property type="entry name" value="MFS_HXT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000044">
        <property type="entry name" value="Hexose transporter Hxt3p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF75">
        <property type="entry name" value="GALACTOSE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
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            <begin position="115"/>
            <end position="135"/>
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    <feature type="transmembrane region" description="Helical" evidence="10">
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            <end position="166"/>
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    <feature type="transmembrane region" description="Helical" evidence="10">
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            <begin position="172"/>
            <end position="194"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
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            <begin position="206"/>
            <end position="227"/>
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    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="239"/>
            <end position="258"/>
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    <feature type="transmembrane region" description="Helical" evidence="10">
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            <begin position="361"/>
            <end position="383"/>
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    <feature type="transmembrane region" description="Helical" evidence="10">
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            <begin position="390"/>
            <end position="408"/>
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    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="428"/>
            <end position="456"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="468"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="496"/>
            <end position="517"/>
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    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="11">
        <location>
            <begin position="70"/>
            <end position="521"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="40"/>
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    </feature>
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        <location>
            <begin position="1"/>
            <end position="13"/>
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    </feature>
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        <location>
            <begin position="21"/>
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        <source>
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        <source>
            <dbReference type="RuleBase" id="RU003346"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR23169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
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    </evidence>
    <sequence length="571" mass="63049" checksum="C4B9AB2E7A61A5D1" modified="2009-07-28" version="1">MSSAPYQQETAATATPVDLHSTVSNGSQLSTPSNKASRDELKAANMAEDAADQVEIPKKPASAYVTVVILCLMVAFGGFVFGWDTGTISGFVNQSDFLRRFGQKHSDGTHYLSNVRMGLIVAIFNIGCAFGGIILSKIGDVYGRRIGLMAVVVIYVVGIIIQIASIDKWYQYFIGRIISGLGVGGIAVLSPMLISETAPKHLRGAMVACYQLMITAGIFLGYCTNYGVKNYSNSVQWRVPLGLCFAWALFMITGMSFVPESPRYLIENGRIEDAKRSLAKSNKVTVEDPAILVEVDLILAGVEAERLAGSASIKELFSTKTKVLQRLIMGIMIQSLQQLTGDNYFFYYGTTIFKAVGLSDSFQTSIVIGIVNFASTFVSLVTVEKFGRRGCMLWGSAAMVACFVVYASVGVTKLYPNGADHPEVTNKGAGNCMICFTCFYIFCFATTWAPIAYVVVSESFPLRVKSKCMAIATASNWLWGFLIAFFTPFITSAIHFYYGYVFMGCLVFAYFYVFFFVPETKGLTLEEVQELWEEGVLPWKSTSWVPSSRRGKDYNVEELKHDDKPWYRRVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHB3</accession>
    <name>C5DHB3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Chromatin modification-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ordered locus">KLTH0E02904g</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Component of an histone acetyltransferase complex.</text>
    </comment>
    <comment type="function">
        <text evidence="12">Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of selected genes principally by acetylation of nucleosomal histone H4 and H2A. The NuA4 complex is also involved in DNA repair. Involved in cell cycle progression and meiosis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Component of an histone acetyltransferase complex. Interacts with H3K4me3 and to a lesser extent with H3K4me2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="16">The PHD-type zinc finger mediates the binding to H3K4me3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the ING family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002553565.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHB3"/>
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        <property type="interactions" value="336"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHB3"/>
    <dbReference type="GeneID" id="8291756"/>
    <dbReference type="KEGG" id="lth:KLTH0E02904g"/>
    <dbReference type="eggNOG" id="KOG1973">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031900_2_0_1"/>
    <dbReference type="InParanoid" id="C5DHB3"/>
    <dbReference type="OMA" id="GPNCKYE"/>
    <dbReference type="OrthoDB" id="5411773at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:meiotic cell cycle"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="ING_ING3_Yng2p"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PHD_ING"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028651">
        <property type="entry name" value="ING_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024610">
        <property type="entry name" value="ING_N_histone-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10333:SF100">
        <property type="entry name" value="CHROMATIN MODIFICATION-RELATED PROTEIN YNG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10333">
        <property type="entry name" value="INHIBITOR OF GROWTH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12998">
        <property type="entry name" value="ING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01408">
        <property type="entry name" value="ING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7 16" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10" id="KW-0469">Meiosis</keyword>
    <keyword evidence="3 14 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14 16" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 15 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="18">
        <location>
            <begin position="205"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="119"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="129"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
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        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
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        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
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        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="13">
        <location>
            <position position="207"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="13">
        <location>
            <position position="218"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="13">
        <location>
            <position position="222"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="13">
        <location>
            <position position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010210"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR628651-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR628651-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU361213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAR23174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="265" mass="30071" checksum="7232C50EFC6E4DE6" modified="2009-07-28" version="1">MDPSSALEQTTHDVSNLQAEFRYILDEIKNADLRVYEQRKKYVQRDAQVQKFVKQHGSGTENPREGELSQKIERELQDCVESQRGKCVLANTALFLVSKHLSKLEKTIRVLEGDNLLAPAEEDKETESGGDNSRESSVMSGERRRKATPTAAVVAPKRKRRADSTPVPRQKDSTPGGGSDANFDLQDYNDDLFSGYNQAEEDDKQLYCFCQSVSYGEMVACDGPNCKYEWFHYTCVNLKEPPKGAWYCPDCRQELANTRLKKRKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHC9</accession>
    <name>C5DHC9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0E03278p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0E03278g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553627.1">
        <property type="nucleotide sequence ID" value="XM_002553581.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHC9"/>
    <dbReference type="STRING" id="559295.C5DHC9"/>
    <dbReference type="GeneID" id="8291772"/>
    <dbReference type="KEGG" id="lth:KLTH0E03278g"/>
    <dbReference type="eggNOG" id="KOG3699">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006033_1_2_1"/>
    <dbReference type="InParanoid" id="C5DHC9"/>
    <dbReference type="OMA" id="GYNEGCA"/>
    <dbReference type="OrthoDB" id="3238794at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.225.10:FF:000009">
        <property type="entry name" value="Class II aldolase/adducin N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="entry name" value="Class II aldolase/adducin N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051017">
        <property type="entry name" value="Aldolase-II_Adducin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001303">
        <property type="entry name" value="Aldolase_II/adducin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10672">
        <property type="entry name" value="ADDUCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10672:SF25">
        <property type="entry name" value="MEIOTICALLY UP-REGULATED GENE 14 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00596">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01007">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="AraD/HMP-PK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Class II aldolase/adducin N-terminal" evidence="1">
        <location>
            <begin position="33"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="268" mass="29159" checksum="4520A352BDAC1E52" modified="2009-07-28" version="1">MPSGNPVLDSATRKSLKAPVFNNLYEKRAWMLEHMAGVLRIFGRKGYNEGCAGHVTIVDPVDPTSYWINPVGVHFSMITVRDLVHVNAKGEAIGGSEAPFSLPGFKIHSQMHKARPEISSICHAHTFYARTFSVFGKKLEMMSQDSCLVHNNYALMADYSGVGLDEVEGDKVSKALGDKMAILLQNHGLMTVGKTVDEAGYLLILLENMCKAQLLADAAAGNSAALKHIIPDNVAKSAFDVISGPDSLYAALQPDYELEVYLSKGDFL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-02-08" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHJ1</accession>
    <name>RRT5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName>Regulator of rDNA transcription protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">RRT5</name>
        <name type="ordered locus">KLTH0E04730g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in the modulation of rDNA transcription.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RRT5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553689.1">
        <property type="nucleotide sequence ID" value="XM_002553643.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHJ1"/>
    <dbReference type="SMR" id="C5DHJ1"/>
    <dbReference type="FunCoup" id="C5DHJ1">
        <property type="interactions" value="213"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHJ1"/>
    <dbReference type="GeneID" id="8291838"/>
    <dbReference type="KEGG" id="lth:KLTH0E04730g"/>
    <dbReference type="eggNOG" id="ENOG502RZDM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042558_0_0_1"/>
    <dbReference type="InParanoid" id="C5DHJ1"/>
    <dbReference type="OMA" id="IWIFRTR"/>
    <dbReference type="OrthoDB" id="439808at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12409">
        <property type="entry name" value="RRM1_RRT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12410">
        <property type="entry name" value="RRM2_RRT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050502">
        <property type="entry name" value="Euk_RNA-bind_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034244">
        <property type="entry name" value="Rrt5_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034247">
        <property type="entry name" value="Rrt5_RRM2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025">
        <property type="entry name" value="OS02G0815200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025:SF1">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000404359" description="Regulator of rDNA transcription protein 5">
        <location>
            <begin position="1"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="RRM 1" evidence="2">
        <location>
            <begin position="21"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="RRM 2" evidence="2">
        <location>
            <begin position="152"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="109"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="271"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="291" mass="32649" checksum="81890C7971637E25" modified="2009-07-28" version="1">MSPTQPNPAAALSGSAEPQTKRIYISNLDFSTTEEELEQFLQEFRVVSVLIPSQTVRGFRNSTVRPLGIGYADFASAADALQAVENLNGKQLRERTLKIKMYVPYSPRARAERRKEKRKVPAPQAEENPDAAPQDAQQPQPPAPAEEPTSKDTVYCAYLPSKVTDVELREFFADYQPQDIYVYRTNGSRRKVYFHRRFTAALVTLGGEAELANAIETLGTRRLMGKKITLRPARLSKVEEVQHAAARKLELEQIQQQTRQIAEHAMAMEEHRQQEAEIPAAETPDDVAATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHY3</accession>
    <name>C5DHY3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Replication protein A subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0E08118g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">As part of the replication protein A (RPA/RP-A), a single-stranded DNA-binding heterotrimeric complex, may play an essential role in DNA replication, recombination and repair. Binds and stabilizes single-stranded DNA intermediates, preventing complementary DNA reannealing and recruiting different proteins involved in DNA metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the heterotrimeric canonical replication protein A complex (RPA).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the replication factor A protein 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553831.1">
        <property type="nucleotide sequence ID" value="XM_002553785.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHY3"/>
    <dbReference type="FunCoup" id="C5DHY3">
        <property type="interactions" value="1254"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHY3"/>
    <dbReference type="GeneID" id="8291990"/>
    <dbReference type="KEGG" id="lth:KLTH0E08118g"/>
    <dbReference type="eggNOG" id="KOG0851">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012393_2_0_1"/>
    <dbReference type="InParanoid" id="C5DHY3"/>
    <dbReference type="OMA" id="DQCDAFY"/>
    <dbReference type="OrthoDB" id="1751331at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005662">
        <property type="term" value="C:DNA replication factor A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007004">
        <property type="term" value="P:telomere maintenance via telomerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd04474">
        <property type="entry name" value="RPA1_DBD_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04475">
        <property type="entry name" value="RPA1_DBD_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04476">
        <property type="entry name" value="RPA1_DBD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000041">
        <property type="entry name" value="Replication protein A subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000064">
        <property type="entry name" value="Replication protein A subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000090">
        <property type="entry name" value="Replication protein A subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047192">
        <property type="entry name" value="Euk_RPA1_DBD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013955">
        <property type="entry name" value="Rep_factor-A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031657">
        <property type="entry name" value="REPA_OB_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004591">
        <property type="entry name" value="Rfa1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00617">
        <property type="entry name" value="rpa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165">
        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165:SF4">
        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08646">
        <property type="entry name" value="Rep_fac-A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16900">
        <property type="entry name" value="REPA_OB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0235">DNA replication</keyword>
    <keyword evidence="7 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="OB" evidence="11">
        <location>
            <begin position="203"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="Replication protein A OB" evidence="13">
        <location>
            <begin position="311"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="Replication factor A C-terminal" evidence="12">
        <location>
            <begin position="469"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="134"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="135"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="150"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF08646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF16900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR23394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="624" mass="70451" checksum="5CE79247D3804780" modified="2009-07-28" version="1">MSDTNLTQGELRGIFTTEEKYHNATGGIYQCVRTRKNDPSNHANRKNLIVVSDGMHRVKALLRNEAVQKAQDAELHSGDIFRLTRGEPAIVKEKKKYVLLIDDLEIIQSRAEIQNPNCELIDNYLIAHPDEVFSSEESAPAQGSSFTGRQQQPQQPQAQNFQKPQQYAPQAQQNYNNSNNFSSSQKSRPIFAIEQLSPYQNMWTIKARVSFKGDIKTWSNQRGEGKLFNVNLLDNSGEIRATTFSENVDKFYNLLQEGKVYYVSKARIQPSKPQFSNLAHPYELQLDRDTVVEECFDDVDVPKMNFSFVKLNTMESQEANSIVDVIGIIRTVNPPFEITSKAGKRFDRRDIILVDDSGYSVNVGLWGQQALEFNLPEGSVVAIKGGRVTDFNGKGLSMGFNSTMHPSPEVPEAYAIKGWYDSVGRNAQFHSLKQEPGAGGGNAARFIANRISIAKAQADNLGMSERGDYFSIKAAVSYLRVDNLAYPACANENCNKKVIEEPDGTWRCEKCDANHPEPQFRYMLTVSVLDETGQIWLTLFNEQAEQLLGVDANSMVKYKESDPSKFARITQKIQMNQYDFRVRARADNYNDETRIRYTAANVHNLRFGAEAEFLAEELGKVFLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DI07</accession>
    <name>C5DI07_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ethanolamine-phosphate cytidylyltransferase</fullName>
            <ecNumber evidence="10">2.7.7.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">CTP:phosphoethanolamine cytidylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0E08712g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from ethanolamine: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytidylyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.14" evidence="10"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553855.1">
        <property type="nucleotide sequence ID" value="XM_002553809.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DI07"/>
    <dbReference type="FunCoup" id="C5DI07">
        <property type="interactions" value="595"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DI07"/>
    <dbReference type="GeneID" id="8292016"/>
    <dbReference type="KEGG" id="lth:KLTH0E08712g"/>
    <dbReference type="eggNOG" id="KOG2803">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031246_2_2_1"/>
    <dbReference type="InParanoid" id="C5DI07"/>
    <dbReference type="OMA" id="FESNNWV"/>
    <dbReference type="OrthoDB" id="40021at2759"/>
    <dbReference type="UniPathway" id="UPA00558">
        <property type="reaction ID" value="UER00742"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004306">
        <property type="term" value="F:ethanolamine-phosphate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd02174">
        <property type="entry name" value="CCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041723">
        <property type="entry name" value="CCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044608">
        <property type="entry name" value="Ect1/PCYT2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45780">
        <property type="entry name" value="ETHANOLAMINE-PHOSPHATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45780:SF2">
        <property type="entry name" value="ETHANOLAMINE-PHOSPHATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="7" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="12">
        <location>
            <begin position="26"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="Cytidyltransferase-like" evidence="12">
        <location>
            <begin position="228"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR23418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="342" mass="38242" checksum="D097D9084C488243" modified="2009-07-28" version="1">MSALAALNCVVNPQLKMENDRHRLWIDGCFDFTHHGHAGAILQARRLIPPGTESGALLCGVHNDEDILFNKGGKPVMNEQERYEHTRSNRWCSEIVKDAPYVTQPDVLDAHRCKFVVHGDDITTDANGEDCYQQMKDMGRFLVVKRTEGVSTTDIIQRILTGARAGAQEARSESSWQDTAGAEEQLKKYAAGLDGHAPWCYVFDGLISKSKIIVEGGYALDSTQLVYIEGDFDLFHVGHIEQLKGLRERHGQDARIIVGVRTTQDCFMTLMERALSVLSCRYVDGLVLDPESQPCVDVTYNLDAAELRRGDFSYLDKAVIVGRVLAEREHYVARNRKKGVCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>C5DI37</accession>
    <name>C5DI37_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0E09438p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0E09438g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553885.1">
        <property type="nucleotide sequence ID" value="XM_002553839.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DI37"/>
    <dbReference type="FunCoup" id="C5DI37">
        <property type="interactions" value="30"/>
    </dbReference>
    <dbReference type="GeneID" id="8292049"/>
    <dbReference type="KEGG" id="lth:KLTH0E09438g"/>
    <dbReference type="HOGENOM" id="CLU_609831_0_0_1"/>
    <dbReference type="InParanoid" id="C5DI37"/>
    <dbReference type="OMA" id="KSGNTHT"/>
    <dbReference type="OrthoDB" id="4036344at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004354">
        <property type="entry name" value="Meiotic_Rec114"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03525">
        <property type="entry name" value="Meiotic_rec114"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01548">
        <property type="entry name" value="MEIOTICR114"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="170"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="269"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="449" mass="50422" checksum="BE6D80AA8F3F8A1F" modified="2009-07-28" version="1">MFDTSVELRRYACYLEEIPAPQGLNSVRGPTNVDKWKHIKNSVKKEFLIRQDNARKFTLQVLVDDVNMERIVVDFGSAVATSSVPGNVAQFSARFPSIMCKYLQVLDNRVVIRRFQMGFMALQDFDRCCNSLQQIGFMLKSASILRADEHFQSRTDSILSSQMPAEISSLTPPSSQFHVQNSFSSSTKRPLDQDSPQAPISRENISNYRKDINQTNGNIQSTAPLDTQTLIGNKVLSRESSSLQEFVKKPTESRVVSTANHFLNPQYLSKEVGDKASSSPRNKPTREETHIPTKGPNLVASSRENTFKLYKKPLDQCVPINVSKSPHMLGEYIDVVSAQSERVAVPEGAPRSSSHQKIFETENQSESSGASVAKLPCSQSLIHPLQGLLGKEDLRQPLSPTNGTLEREAIPTRPALTKDLLKERIKDQNFMQWVCQAFRSHFDYLISRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-04-02" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>C5DI79</accession>
    <name>C5DI79_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0E10384p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0E10384g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553927.1">
        <property type="nucleotide sequence ID" value="XM_002553881.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DI79"/>
    <dbReference type="GeneID" id="8292092"/>
    <dbReference type="KEGG" id="lth:KLTH0E10384g"/>
    <dbReference type="HOGENOM" id="CLU_1027002_0_0_1"/>
    <dbReference type="InParanoid" id="C5DI79"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="150"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="182"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="225"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="271" mass="29217" checksum="ECD454FE4422DC91" modified="2009-07-28" version="1">MRKTFNEDECACPPPRASHASRCYRRLPVRTHFVTYAHRRWTHRNCVTCSEGPRARRCTRKDAGLSAALLTTQLPLGATPATRSAAGANGADAGAGTGEASSTGADVLVFLRCLQQALEGPGRLYIIFPDLPKQMESRWKVDGRKEKRATGGTRADGAAAARVEPSPSYRRDASSSVATGRRSGDAMEGKLKPRQTGGQRLGARRETEVQGCGGEQQVTRKSRAQARAGRGRISARGEARAAGRRGVRPRPRDAHVTGECHMTSHMTATIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DI93</accession>
    <name>C5DI93_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">mRNA export factor GLE1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Nuclear pore protein GLE1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Nucleoporin GLE1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">RNA export factor GLE1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">KLTH0E10692g</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Nucleoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the GLE1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553941.1">
        <property type="nucleotide sequence ID" value="XM_002553895.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DI93"/>
    <dbReference type="FunCoup" id="C5DI93">
        <property type="interactions" value="143"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DI93"/>
    <dbReference type="GeneID" id="8292106"/>
    <dbReference type="KEGG" id="lth:KLTH0E10692g"/>
    <dbReference type="eggNOG" id="KOG2412">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029651_0_0_1"/>
    <dbReference type="InParanoid" id="C5DI93"/>
    <dbReference type="OMA" id="VPANIHS"/>
    <dbReference type="OrthoDB" id="420884at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044614">
        <property type="term" value="C:nuclear pore cytoplasmic filaments"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000822">
        <property type="term" value="F:inositol hexakisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.510:FF:000003">
        <property type="entry name" value="Nucleoporin GLE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.510">
        <property type="entry name" value="GLE1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012476">
        <property type="entry name" value="GLE1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038506">
        <property type="entry name" value="GLE1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12960">
        <property type="entry name" value="GLE-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12960:SF0">
        <property type="entry name" value="MRNA EXPORT FACTOR GLE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07817">
        <property type="entry name" value="GLE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="10" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="83"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="177"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00026227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00075092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00075681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAR23504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="522" mass="60454" checksum="22FE5B29597BFB68" modified="2009-07-28" version="1">MRFNYEELCNIDSPEWHDEDEYTVIGGDDEHVPQLRLPRHVKRDTVPSDSIGVLDSGLAELMNELDLGSKIPGPSRLKPGIWRKRTKSLSKSESKPFNPTPARHSYEPEMFLNLRESLAKSILMKLETLERDNAERVQEVKNEKERIRMEQQRIEEEQKRKAEEQARIRREEAEKLRQRAEMERKQQLEEQKRKEAERKRLEEERRKQEAAEKELKAQEEAKKVFGLTNFSKVEDFFKHYKHRIKAIKSEIVEPVKKDTSLKSILSKHKRKINPKFGQLTNSEQHLATIFSELSSLVDETKANQLGYQWILNFIAKALVSQAETEVRVKPESALPLGKLALNLLARYPELLDFLMARFVKKCPLVIGFTCNINSEEGRYKMGWKRAQNDTWEEETSYDERMGGITTLFSVITRLPLPLEIISSQAHPLPIVHSWQMLARIANTHTDLLTNTHFVVLGSWWDAAAAQFVQAYGNQARKLMSLLTNDLTNAVAERKYVGAARLRILSEDYETSGTLKSFPEMNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIK5</accession>
    <name>C5DIK5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Nucleolar GTP-binding protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0E13244g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">Involved in the biogenesis of the 60S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 8">Nucleus</location>
            <location evidence="2 8">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family. NOG subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554053.1">
        <property type="nucleotide sequence ID" value="XM_002554007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIK5"/>
    <dbReference type="FunCoup" id="C5DIK5">
        <property type="interactions" value="1436"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIK5"/>
    <dbReference type="GeneID" id="8292222"/>
    <dbReference type="KEGG" id="lth:KLTH0E13244g"/>
    <dbReference type="eggNOG" id="KOG1490">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011784_4_1_1"/>
    <dbReference type="InParanoid" id="C5DIK5"/>
    <dbReference type="OMA" id="EWKNDVM"/>
    <dbReference type="OrthoDB" id="415015at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902626">
        <property type="term" value="P:assembly of large subunit precursor of preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01897">
        <property type="entry name" value="NOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1190:FF:000001">
        <property type="entry name" value="Nucleolar GTP-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000496">
        <property type="entry name" value="Nucleolar GTP-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024926">
        <property type="entry name" value="NOG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041623">
        <property type="entry name" value="NOG1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010674">
        <property type="entry name" value="NOG1_Rossman_fold_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012973">
        <property type="entry name" value="NOG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45759">
        <property type="entry name" value="NUCLEOLAR GTP-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06858">
        <property type="entry name" value="NOG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17835">
        <property type="entry name" value="NOG1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08155">
        <property type="entry name" value="NOGCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038919">
        <property type="entry name" value="NOG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="OBG-type G" evidence="10">
        <location>
            <begin position="168"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="533"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="544"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="598"/>
            <end position="626"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR038919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR23616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="647" mass="73548" checksum="D9815AE113D0AFE1" modified="2009-07-28" version="1">MQLSWKDIPTVAPANDLLDIVLNRTQRKTPTVIRPGFAITRIRAFYMRKVKFTAEGFEEKFEDILKGFPNINDVHPFHRDLMDTLYEKNHYKVSLAAVSRAKTLIEQVSRDYVRLLKFGQSLFQCKQLKRAALGRMATIVKKLKDPLVYLEQVRQHLGRLPSIDPNTRTLLICGYPNVGKSSFLRCITKADVEVQPYAFTTKSLYVGHFDYKYLRFQAIDTPGILDRPTEEMNNIEMQSIYAIAHLRSCVLYFMDLSEQCGFTIEAQAKLFHSIKPLFANKSVMVVINKTDIIRPEDLDEERAALLKGIADMPGVEIMPTSCVEEDNVMEVRNKACEKLLASRIENKLKSTARVNNVLNKIHVAKPQARDDGVDRSPYIPDAIKNMKKFDQDDPNRMKLAREVEAENGGAGVFNINLKDKYLLEDDEWKNDVMPEILDGKNVYDFMDPEIAAKLQALEEEEEKLEAEGFYNSDEEEEAYEGFAADEVADIKEKAAWIRDRQKKMIVAARNRKALKNRAVMPRSKLTKSFGEMEKHMSSLGHDMSSLQDKQKSAAQRNKYVESGADVVFGGADTTNTSSNGGKLRQSDRLLDGVADGTMRSKADRLAKIQRRERNREARAGEADRHSTAALAKHLFSGKRGIGKTDFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIN5</accession>
    <name>C5DIN5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0E13904p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0E13904g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554083.1">
        <property type="nucleotide sequence ID" value="XM_002554037.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIN5"/>
    <dbReference type="GeneID" id="8292252"/>
    <dbReference type="KEGG" id="lth:KLTH0E13904g"/>
    <dbReference type="eggNOG" id="ENOG502S7MJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_162829_0_0_1"/>
    <dbReference type="InParanoid" id="C5DIN5"/>
    <dbReference type="OrthoDB" id="4066074at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="125" mass="13886" checksum="D26181473DEBD3DF" modified="2009-07-28" version="1">MSHSIIVSNVPVGVSDVSVMRYIREMTGNTPVVHMQPFPDECHYVNDSNATKTLQIFFATAEEAAIANSLFQGSSVEEDLTRVHAMFHGGRDEHVPRSLASQDTPWNETQSKQPAQRSRIKSLST</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIX5</accession>
    <name>C5DIX5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0E15928p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0E15928g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554173.1">
        <property type="nucleotide sequence ID" value="XM_002554127.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIX5"/>
    <dbReference type="FunCoup" id="C5DIX5">
        <property type="interactions" value="65"/>
    </dbReference>
    <dbReference type="GeneID" id="8292344"/>
    <dbReference type="KEGG" id="lth:KLTH0E15928g"/>
    <dbReference type="HOGENOM" id="CLU_065220_0_0_1"/>
    <dbReference type="InParanoid" id="C5DIX5"/>
    <dbReference type="OMA" id="LLIHYIE"/>
    <dbReference type="OrthoDB" id="4031722at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="311" mass="34237" checksum="4B89BEF5A90277B5" modified="2009-07-28" version="1">MGHSASKESEPTDQLAGAGNAPVFGRERELVLASVVFQRKVLMCFASARSFDCYARGDGEFQIRDDGLGFPTLAMERAHPLQCLLKARDPAFRIYQFFLRTASEPAPHPECRIACHSGDKLIYKAPFCDVYELDSPVRDAGYELSFPNMPDTTRVRLERCKGSLHMSADLGGWNTQWICTSGSPLHPKELLLVVTEGGRDMPSRIRLPSSLSARQWAKYREFFSVLPSRSAEKVASLCVRDAEVSEAEVSTCGIGEVPWYTGVIGCMGLIMCHLHEQKKRNRGKNNPFSAANHLGSAPFIASSVMGSPASC</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIY7</accession>
    <name>C5DIY7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0E16214p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0E16214g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554185.1">
        <property type="nucleotide sequence ID" value="XM_002554139.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIY7"/>
    <dbReference type="FunCoup" id="C5DIY7">
        <property type="interactions" value="308"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIY7"/>
    <dbReference type="GeneID" id="8292357"/>
    <dbReference type="KEGG" id="lth:KLTH0E16214g"/>
    <dbReference type="eggNOG" id="ENOG502S4M0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_270952_0_0_1"/>
    <dbReference type="InParanoid" id="C5DIY7"/>
    <dbReference type="OMA" id="GDWDKSY"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051240">
        <property type="entry name" value="Mito_RNA-Proc/Resp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013888">
        <property type="entry name" value="RNase_P_Rpm2_mt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47933">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47933:SF11">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08579">
        <property type="entry name" value="RPM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="78"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="78"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR23748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1193" mass="137188" checksum="71CC0BBE266F1CDD" modified="2009-07-28" version="1">MAFKSFKCKFYSKGYHSSAQKQTTAFFESSYQYLRRNQGVVSQESHIPSSHNVVVSPNPVVVANVNYNTLEHVLETEAAEEKAHRTEGELNGSEQRSSKHGRRASLSGAGGSGQARDRYRAFNADSIPHYSNNRHLVINERLSKSYFSTTAVEPSNIPGQEQNLVTPSATADPKAVLDADASNPVSANSQASPWEPDTEETLNPETFLQTQVAHIKHCFAVGDYNKINALYQALVRNEIVPPVEIYATVIESVCMRDLDNDNVDNRMFQLLNCYQDLINNKLKPTEKIYSLVIGSLLKGSILAYESGNGNGCDFYKIAVDLFRASNAHKSHEFPKELLDCSLLAMNLYPGYVELDYVIEVLNNSKLYVRDTFYYVSMMSYAKHLNNNFVLKKLYDEFRSACVADVNLQNHQYEVYSIMLSGLVETGDMALAVKLMDKVLTDAKEKVGLASNISMVLSNFLISVSKMDCQKAYQLWLEFKKLQWIPEFSYSFYMTLLSNSLGDWQLARKIYDYMLPMTREPNKPPASLIERLLQPLGTNSLLSSFLDYALQLNDTEVVMKVLEESIVKRFGFEIGVYPYVFQFLRNIRCPDDYLLRFINFHGQLLTKGNERFEFLNGLIDSFQSQVILAKVTEMKFFADYCRSFNVAESRMINHSGIIACFQSLWGSPQTIERYSYNIELHGIMVCKLHDLEGYYAVMENEYLLQFKDRLTERFEKLMINYKRLNLDPNMISGTPVQAAKMISMSEELINYFAHPGDWDKSYPLCLGSMLRNSFSTGLKAYERLSDDGFCFDYDTYRELIKQKVNNSEIITKALDLCPDEEEERYLSNCLVVKTYSKELEDKVLKHPLFKTKILPHLKDSFYVRLAKNTSDFQFFIEQIGFPQRFRSIADQVEHKEIVSYIYERLFKDKRYDEVLASNNVCPVLNVELLLKSCIRAGEFGQYKTIFNKFKDSLVDASLNVQAEYLITNKRVDEAIKLIRGSSQKTEHKTNDLLSFALFLKSFSEPIMNFDLIENTLQLANILSSQDAFASMVAFYQTLMHDSAFLHGVNMNQAMSCEIVQQMLNNLGDAVQFVDLENENVRKNFQRKINNYFRFRMFLKLPNLEECTVLRIIQIYAKVQPQAIDALFNNIVETIYLNPNTRLLYLRNDMVFNFKPQQLASVVREIEDIYISNNELEDAEKAKTFNGVLEKLYSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIZ1</accession>
    <name>C5DIZ1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0E16302p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0E16302g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554189.1">
        <property type="nucleotide sequence ID" value="XM_002554143.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIZ1"/>
    <dbReference type="STRING" id="559295.C5DIZ1"/>
    <dbReference type="GeneID" id="8292361"/>
    <dbReference type="KEGG" id="lth:KLTH0E16302g"/>
    <dbReference type="eggNOG" id="KOG2202">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059852_3_1_1"/>
    <dbReference type="InParanoid" id="C5DIZ1"/>
    <dbReference type="OMA" id="RNPRHHE"/>
    <dbReference type="OrthoDB" id="423462at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0089701">
        <property type="term" value="C:U2AF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12287">
        <property type="entry name" value="RRM_U2AF35_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009145">
        <property type="entry name" value="U2AF_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12620">
        <property type="entry name" value="U2 SNRNP AUXILIARY FACTOR, SMALL SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01848">
        <property type="entry name" value="U2AUXFACTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="8">
        <location>
            <begin position="22"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="49"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="8">
        <location>
            <begin position="142"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="6">
        <location>
            <begin position="22"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="6">
        <location>
            <begin position="142"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR23752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="189" mass="22535" checksum="11958C816671C13F" modified="2009-07-28" version="1">MVRVLRFYIGLRIAILTNNHLVSHFYYKIGACRHGDRCSKKHIRPLHSPTVLVPNMYRRPESEGPEAQQRDFDAFYEDVYMEACKFGELQAMVVCENKNDHLNGNVYLMFTNSRDANSAKDSFNTRWFNERPLYCEFSHVSDFREAICRKHDMRSCERGDECNFMHVQRPTRDLQSDLERAQWKEYHIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJ75</accession>
    <name>C5DJ75_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">KLTH0F14102p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0F14102g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554801.1">
        <property type="nucleotide sequence ID" value="XM_002554755.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJ75"/>
    <dbReference type="FunCoup" id="C5DJ75">
        <property type="interactions" value="71"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJ75"/>
    <dbReference type="GeneID" id="8293027"/>
    <dbReference type="KEGG" id="lth:KLTH0F14102g"/>
    <dbReference type="eggNOG" id="KOG0769">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015166_6_3_1"/>
    <dbReference type="InParanoid" id="C5DJ75"/>
    <dbReference type="OMA" id="PLEMINT"/>
    <dbReference type="OrthoDB" id="446044at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015217">
        <property type="term" value="F:ADP transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005347">
        <property type="term" value="F:ATP transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045900">
        <property type="entry name" value="Peroxisomal_Ade_carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46650">
        <property type="entry name" value="PEROXISOMAL ADENINE NUCLEOTIDE TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46650:SF1">
        <property type="entry name" value="PEROXISOMAL ADENINE NUCLEOTIDE TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="119"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="225"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR24364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="326" mass="36365" checksum="28E27B24A5DCFF35" modified="2009-07-28" version="1">MASLESAITGAVASSLANVLVYPLDVVKTLIQTQNKSSGEATVDAEGAGKRETQETRYRNTLDAIIKIIKNRGIGGLYRGLPASIVAGFLQSFSYFFWYSVVRKSFFRLKLLKGKITKFSTPEELLLGIVAAAVSQVFTNPVGVISTRQQTLEGNNKAKFVDVVRQIYKEQQNISGFWRGLKVSLILTINPSITFASYERLKDIFISSSAQSQNDGKLMETSAQLSPAQNFALGFISKMISTLITQPLIISKAYLQRTGSQFQSFQQVLHYLYTQEGFLALWKGIAPQLCKGLLVQGLLFMFKGELTKLMRRLFRYIKANRRARLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJA8</accession>
    <name>C5DJA8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0F14916p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0F14916g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RutC family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554834.1">
        <property type="nucleotide sequence ID" value="XM_002554788.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJA8"/>
    <dbReference type="GeneID" id="8293063"/>
    <dbReference type="KEGG" id="lth:KLTH0F14916g"/>
    <dbReference type="eggNOG" id="KOG2317">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100715_7_2_1"/>
    <dbReference type="InParanoid" id="C5DJA8"/>
    <dbReference type="OMA" id="DEYNTAY"/>
    <dbReference type="OrthoDB" id="309640at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019239">
        <property type="term" value="F:deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00448">
        <property type="entry name" value="YjgF_YER057c_UK114_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.40">
        <property type="entry name" value="RutC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035959">
        <property type="entry name" value="RutC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006175">
        <property type="entry name" value="YjgF/YER057c/UK114"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11803">
        <property type="entry name" value="2-IMINOBUTANOATE/2-IMINOPROPANOATE DEAMINASE RIDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11803:SF58">
        <property type="entry name" value="PROTEIN HMF1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01042">
        <property type="entry name" value="Ribonuc_L-PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55298">
        <property type="entry name" value="YjgF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="151" mass="16691" checksum="23FF350E067F774B" modified="2009-07-28" version="1">MIQTLHPLLTSCVRKMAKKVTWQEVGATKGIDLLSPAFVTSGSKLVFTSGCIGSDPVTDELPEDLEQQARNALQNLRNVLEASGSNSDRVLKVLLFVADGAYSQTVNRIYREQFPNSPARSCVVVSFPNPKIKVELECIAEVQPKARWFKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJB7</accession>
    <name>C5DJB7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0F15114p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0F15114g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554843.1">
        <property type="nucleotide sequence ID" value="XM_002554797.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJB7"/>
    <dbReference type="FunCoup" id="C5DJB7">
        <property type="interactions" value="374"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJB7"/>
    <dbReference type="GeneID" id="8293072"/>
    <dbReference type="KEGG" id="lth:KLTH0F15114g"/>
    <dbReference type="eggNOG" id="KOG3771">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025518_0_1_1"/>
    <dbReference type="InParanoid" id="C5DJB7"/>
    <dbReference type="OMA" id="QEYDYYN"/>
    <dbReference type="OrthoDB" id="2159336at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031097">
        <property type="term" value="C:medial cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990528">
        <property type="term" value="C:Rvs161p-Rvs167p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051666">
        <property type="term" value="P:actin cortical patch localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097320">
        <property type="term" value="P:plasma membrane tubulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07599">
        <property type="entry name" value="BAR_Rvs167p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000048">
        <property type="entry name" value="BAR adaptor protein RVS167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000100">
        <property type="entry name" value="SH3 domain-containing YSC84-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046982">
        <property type="entry name" value="BIN3/RVS161-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47174">
        <property type="entry name" value="BRIDGING INTEGRATOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47174:SF1">
        <property type="entry name" value="REDUCED VIABILITY UPON STARVATION PROTEIN 167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03114">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00721">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51021">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="BAR" evidence="6">
        <location>
            <begin position="17"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="375"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="290"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="25"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="294"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="320"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="336"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR24406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="435" mass="49611" checksum="1057BED983FC1B87" modified="2009-07-28" version="1">MSFKGLTKAVTRAPQNFRQKFNMGQQTEDAVYEDAERRFKELETETKKLSDESKRYFSAVNGMLNHQIGFAKAMEEIFKPISGKISDPNASIPEDNPEGIEASEQYRTLVAELQATLKPDLSLIEEKIVKPSQELLKVIDYIRKMATKRNHKKLDLDRHLNTYSKYENKKEPTPKDEEKLYKAQAQMEVAQQEYDYYNDMLKTELPLLFQLEAEFVKPLFVSFYYMQLNIFYSLYNRMQDMKIPYFDLDTDIMEAFVAKRGNIEEQTDSLTITRFRVGYGKAKLEMTKKRYGVQSPTSPGSPVSAQSPVFASTPTTPSFAQPQQQQQQYAPPPYGQPQVAGAQQQYQYPAEKASYTPQPASAYTAYTPPTTAPASAPETVTALYDYQAQAEGDLTFPAGAIIEVVERTNDTNGWWTGRYNGYQGVFPGNYVQLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJF9</accession>
    <name>C5DJF9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0F16082p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0F16082g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554885.1">
        <property type="nucleotide sequence ID" value="XM_002554839.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJF9"/>
    <dbReference type="FunCoup" id="C5DJF9">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJF9"/>
    <dbReference type="GeneID" id="8293116"/>
    <dbReference type="KEGG" id="lth:KLTH0F16082g"/>
    <dbReference type="eggNOG" id="ENOG502QT2Z">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103819_1_0_1"/>
    <dbReference type="InParanoid" id="C5DJF9"/>
    <dbReference type="OMA" id="FNCILAS"/>
    <dbReference type="OrthoDB" id="3338076at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070916">
        <property type="term" value="C:inositol phosphoceramide synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070917">
        <property type="term" value="F:inositol phosphoceramide synthase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006673">
        <property type="term" value="P:inositol phosphoceramide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013862">
        <property type="entry name" value="Kei1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28077">
        <property type="entry name" value="INOSITOL PHOSPHORYLCERAMIDE SYNTHASE REGULATORY SUBUNIT KEI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28077:SF1">
        <property type="entry name" value="INOSITOL PHOSPHORYLCERAMIDE SYNTHASE REGULATORY SUBUNIT KEI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08552">
        <property type="entry name" value="Kei1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="206" mass="23566" checksum="64684BA4540D3DA3" modified="2009-07-28" version="1">MGVEIGLGITILNKCSGFYGILGLLTGHQLDFMQWVLYLTSVFTLVVYSLGLTTVYKPKLQTYAFVLLTFTADTLITCFFTLWFSGMWFAAKESELSDPSSATQQTSGGISSGSKLLTARGETLSSQSASQATEYFFTILVSLIALVSRFYFNFIILAFVQRLFRHPKYLVDQDDVDQDLKHKKLWQRWWIRAENWSYRVCHHYLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJJ2</accession>
    <name>C5DJJ2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Casein kinase II subunit beta</fullName>
            <shortName evidence="3">CK II beta</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0F16852g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 3">Regulatory subunit of casein kinase II/CK2. As part of the kinase complex regulates the basal catalytic activity of the alpha subunit a constitutively active serine/threonine-protein kinase that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the casein kinase 2 subunit beta family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554918.1">
        <property type="nucleotide sequence ID" value="XM_002554872.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJJ2"/>
    <dbReference type="FunCoup" id="C5DJJ2">
        <property type="interactions" value="1134"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJJ2"/>
    <dbReference type="GeneID" id="8293151"/>
    <dbReference type="KEGG" id="lth:KLTH0F16852g"/>
    <dbReference type="eggNOG" id="KOG3092">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034027_3_2_1"/>
    <dbReference type="InParanoid" id="C5DJJ2"/>
    <dbReference type="OMA" id="DADFGRC"/>
    <dbReference type="OrthoDB" id="3971593at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032545">
        <property type="term" value="C:CURI complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005956">
        <property type="term" value="C:protein kinase CK2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034456">
        <property type="term" value="C:UTP-C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030291">
        <property type="term" value="F:protein serine/threonine kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042790">
        <property type="term" value="P:nucleolar large rRNA transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060962">
        <property type="term" value="P:regulation of ribosomal protein gene transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006356">
        <property type="term" value="P:regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006359">
        <property type="term" value="P:regulation of transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1820.10:FF:000005">
        <property type="entry name" value="Casein kinase II subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.20:FF:000001">
        <property type="entry name" value="Casein kinase II subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1820.10">
        <property type="entry name" value="protein kinase ck2 holoenzyme, chain C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016149">
        <property type="entry name" value="Casein_kin_II_reg-sub_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035991">
        <property type="entry name" value="Casein_kinase_II_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000704">
        <property type="entry name" value="Casein_kinase_II_reg-sub"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11740">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11740:SF39">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01214">
        <property type="entry name" value="CK_II_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00472">
        <property type="entry name" value="CASNKINASEII"/>
    </dbReference>
    <dbReference type="SMART" id="SM01085">
        <property type="entry name" value="CK_II_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57798">
        <property type="entry name" value="Casein kinase II beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01101">
        <property type="entry name" value="CK2_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00045899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR24481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="241" mass="27954" checksum="01117E12A4E478D6" modified="2009-07-28" version="1">MADVEMTEARLDTAEGSISSEYVEFWIDLFLGRKGHEYFCDVDTDYITDRFNLINLQKTVNKFTQVIQYIVDELDDGVLEQMSSARLEQLEHDARKLYGLIHARYVITIKGLQKMLQKYKEADFGRCPRVYCNFQPLLPVGLHDTPGVDCVKLYCPCCEDLYIPKSSRHSAIDGAYFGTSFPGMFLQAFPEMVPPRPVKRYVPRIFGFELHKQAQVARWQELQRRKLVHSLEQRGIDGGWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJP7</accession>
    <name>C5DJP7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0F18128p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0F18128g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554973.1">
        <property type="nucleotide sequence ID" value="XM_002554927.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJP7"/>
    <dbReference type="FunCoup" id="C5DJP7">
        <property type="interactions" value="320"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJP7"/>
    <dbReference type="GeneID" id="8293208"/>
    <dbReference type="KEGG" id="lth:KLTH0F18128g"/>
    <dbReference type="eggNOG" id="KOG3907">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028824_3_0_1"/>
    <dbReference type="InParanoid" id="C5DJP7"/>
    <dbReference type="OMA" id="DDFPSIC"/>
    <dbReference type="OrthoDB" id="19859at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046873">
        <property type="term" value="F:metal ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006829">
        <property type="term" value="P:zinc ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045891">
        <property type="entry name" value="ZIP9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16133">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 39 ZINC TRANSPORTER , MEMBER 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16133:SF0">
        <property type="entry name" value="ZINC_IRON REGULATED TRANSPORTER-RELATED PROTEIN 102B, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="76"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="162"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="195"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="229"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="298"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR24536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="319" mass="34059" checksum="13BC1A4F05C8C14F" modified="2009-07-28" version="1">MSVTLSEVLVTAFLFLTVVFAIGLVPIYLVGRRDAGESKYLAMFSLFGVGMLLGTSFMLVIPEGVEACLEHNGNVGLNLLIGFMVVFFLDNVASAVAKGAKTSRLSSFLQDAPEMNSWKDVLKPKKALTLVVRNQVVFALVVHGCSDGLAMGAATSNDSVKFATLIAIIIHKIPAVLSLSSVMVTKQHLPALEAVSNLLAFSMSTPGAYIVISALNLKKWEALEWVSGNILLMSGGSLLYAAVSALLGGHDRTPETHEVRGAEPLMSSSLDQEFTSKDMTPFEIVSSPVKEEVPHGETLYVVAGVIIPVIVSYLISEKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJQ1</accession>
    <name>C5DJQ1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Mediator of RNA polymerase II transcription subunit 17</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7 8">Mediator complex subunit 17</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">MED17</name>
        <name evidence="10" type="ordered locus">KLTH0F18216g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Mediator complex subunit 17 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJQ1"/>
    <dbReference type="FunCoup" id="C5DJQ1">
        <property type="interactions" value="191"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJQ1"/>
    <dbReference type="KEGG" id="lth:KLTH0F18216g"/>
    <dbReference type="eggNOG" id="ENOG502QS9H">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023188_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJQ1"/>
    <dbReference type="OMA" id="PKINDKR"/>
    <dbReference type="OrthoDB" id="5319830at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019313">
        <property type="entry name" value="Mediator_Med17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13114">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13114:SF7">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10156">
        <property type="entry name" value="Med17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="108"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="50"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="124"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR24540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="659" mass="74141" checksum="842A4536D9134EBB" modified="2009-07-28" version="1">MAGEEPQANGFRSDSTYKKPLAIDPNLIRPVPSFASPTADSDNDHKDDQSQPSTGVTQPLVNNPYETFGNMPLDQLIPLILQSRGPGTKFADLTEESLMEEIMKDAPLPQAQQDSDGDLDMDGSQLTNDPGQRTPEPQQIEGPAQADEDAVLSQEQFRQLKASTVQSLNLAVNESSLALEFVSLLLSSVRPSAANASMSPFLKKTVPPASLNAEKIPFQKPSSDEVLERSLIAKGWKQRSLEESRTLLKDSYLALEKSLKKEHSYWSKVSHNISKKDVVFKMKDRETGEKSLGIKYGYEDSGSTYTQERGIAVLRHNSKLDNLELVPADRQQANALGKENNEKFVRVRIFTKIEEEDDYILTGESHLDKLLIPNQVAGEQNDFRRQISRLKFFIFEKDLMYQLKRESENLISYGVSIENENKITIEFPTEKLELETMHVNDDVIMNHQQDAPKTNDRKATLVLIMLRMLLVVIYKKQLRKKLLCPQTSIGASAKSPKVHKVVGRLQDNEVLLLRPIIGKFKHESYVSLLKKIIKGFVLDVVEGSSVRKVENPSNTVTIKDEVVYDNHIAKLDKEIHLFDKILEMPRSDLLVETPKAGTILFLLQSSNYCNATTGVKYTNGAGETLFDTSFSNFKELEDFLHFIVSEYVSGGQENGVKYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJQ3</accession>
    <name>C5DJQ3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">KLTH0F18260p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0F18260g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554979.1">
        <property type="nucleotide sequence ID" value="XM_002554933.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJQ3"/>
    <dbReference type="FunCoup" id="C5DJQ3">
        <property type="interactions" value="56"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJQ3"/>
    <dbReference type="GeneID" id="8293214"/>
    <dbReference type="KEGG" id="lth:KLTH0F18260g"/>
    <dbReference type="eggNOG" id="KOG1246">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000991_4_0_1"/>
    <dbReference type="InParanoid" id="C5DJQ3"/>
    <dbReference type="OMA" id="TFPKCYH"/>
    <dbReference type="OrthoDB" id="1678912at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034647">
        <property type="term" value="F:histone H3K4me/H3K4me2/H3K4me3 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15544">
        <property type="entry name" value="PHD_BAZ1A_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.650:FF:000055">
        <property type="entry name" value="Jhd2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003349">
        <property type="entry name" value="JmjN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694:SF33">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02375">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00545">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51183">
        <property type="entry name" value="JMJN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="JmjN" evidence="10">
        <location>
            <begin position="7"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="9">
        <location>
            <begin position="259"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="JmjC" evidence="11">
        <location>
            <begin position="407"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="232"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="241"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR24542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="799" mass="92810" checksum="DA1743D041867B79" modified="2009-07-28" version="1">MVELEHIPVLHPTAAEMEDPIGFLSQTHVRRLGHVYGMVKLVPPSDFRPPLSINEDQFKFRVRLQYLNELNILNRGRLFLMKQLNNFYMRGSRRTEKHLRKPYTDVGDKRLFYYDLFITIAKYFNPSKSLAPERNMGRKRPRFSAADLESQTAAKSGSALKLAPLSEIENNQTLWKHVAKELSVPQVSAKDAFFSILAPYYEFLQKRTREHDGSGALLSKLIYAEKFPNSLLNDMNHGEEGEPADGDDEEEEEEEEEEEEGCTLCQRTNKRTKTILCDSCDKPFHIFCLDPPLKEVPKGKWVCNNCIFGNGYYGFKEEDKFYSRTAFQKMCREYDAERWPHGDKLNNLEHLEKMFWDKVEQIDKSSPIRYGADIHNVGPGEMTGFPTAQYIPSAMKDDNIAHKQYLEYVRHPMNLVNLPSAKGSLLSVFGKKISGMTVPWIYIGSTFSTFCWHLEDQYTLSANYQHEGDPKIWYSIPESSCERFDKLMRDTAPDLFQKQPDLLHQLVTLIAPYDKRFQEAKISCFKAIQYPGEYIITFPKCYHSGFNSGYNFNEAVNFTLDLWLPYGIEATRDYVGSGKRCVFDMWELMLTVLVEYLNDSKKFDESLIRSCHTELEAMFNQEVKSIDQLSKILNNRVEATGITRRRISDKTQVDVKVTVRHDYREPDFEDDEHTSASDNEFGSDDDGDDDIFCAACKTICPFFFVAHFEKSRVSKRRRLQSMGPAEWNQMSERGEIKIFCLRDYIKLLELESEGDQSDCSDDENNLKKFQRDELFLIRKPGEIRDVLKEVQHKLDGMLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJZ1</accession>
    <name>C5DJZ1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0F00484p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0F00484g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554229.1">
        <property type="nucleotide sequence ID" value="XM_002554183.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJZ1"/>
    <dbReference type="FunCoup" id="C5DJZ1">
        <property type="interactions" value="307"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJZ1"/>
    <dbReference type="GeneID" id="8292420"/>
    <dbReference type="KEGG" id="lth:KLTH0F00484g"/>
    <dbReference type="eggNOG" id="ENOG502QSP5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007004_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJZ1"/>
    <dbReference type="OMA" id="RAPRILM"/>
    <dbReference type="OrthoDB" id="2130597at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018564">
        <property type="entry name" value="Repl_chkpnt_MRC1_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09444">
        <property type="entry name" value="MRC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA replication checkpoint mediator MRC1" evidence="2">
        <location>
            <begin position="648"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="225"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="268"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="454"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="595"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="793"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="960"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="306"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="471"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="595"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="813"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="960"/>
            <end position="976"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR23792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="993" mass="111147" checksum="4B5457B1B76F4DEE" modified="2009-07-28" version="1">MDLIDNLRARNRGSGTAKKVKERGCDSLPLVTGTGFLFGNSVLRRVKSRLGDDENHEADSTKSPIDNGCCDLSQTQVVGLAYDGEDLEQDFADSTARIEGKSWAGPTNKPNYPKDKRRVSSNNSTVERRIPRSELESTEVNTQLISPHQVKEIDELETQLLLPDVDLHPTNQSSNNTILDGTITQISGFGETCHDNAIQATSKDVSDTSTFVPITTKTKYSNSSLSFNANDPEEESNTIAMSPADATERDRTIRKRFDAQSFIESFDDETDELQSCAASNASETEAPVLKQLEDGEKNANNLKKPTHFESNRENNPNKDSSSIKIFPTMFEENKADASAIILSSSSEEEEDYESKKPSFKAAILDLKARLSKKSQSLLIPRSKVTSNFSSHQILFASLRKANKSQILDYRKEKYQSKGIDYDKITEEKNSIESLLERELARNKKIRLREIEQERQNERKDFGEQSSAFDYSNDELEEEPSDEEIDENQSSSPVSLHSGIVCADDAVECAANRMQDLKQTIKPNEEEDAIRKSGLRKHKKFCLIDTDEEDGSVLNSGNIIDLGAYGSNIGNLNQIEQEQVGEHVGAHIENDLTTTGRNKNLIKPSSRSSSFQVEDNSHEDMNPGVIRELIEKHKRKELLREAKLEKLHQSKASRIIDYEAEESDDEWHGIGGVDGERFDDHDSDLEKMIDDYSNSRFDSSEVRKRQIEEEISEDKSMVNKILHDIETGGFRKRGRNALDLELSDDDDEELLKYHSRRKELLRQKVSAQGEAKLLAENPKSKAFFETIVEDIRSKGALEDEGPPPVRGFSSVNAPEEKNSDSDKKGKKTVLSEAFVQQTLSFLTSGEVGEEKGPENNLGSLPTHVPSFNTEETQDIFALKQNSSIKSLSAPTRNSSNMLIDDQEDLLSRKRACSFFARFTKRVDANEKFEEGKKTVRSLNSYKVAGSSKASITYLGKARKLNAPKKVAHQQSRKRGHKPAAGFGIFASNSESFES</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKE9</accession>
    <name>C5DKE9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0F04114p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0F04114g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554387.1">
        <property type="nucleotide sequence ID" value="XM_002554341.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKE9"/>
    <dbReference type="FunCoup" id="C5DKE9">
        <property type="interactions" value="31"/>
    </dbReference>
    <dbReference type="GeneID" id="8292582"/>
    <dbReference type="KEGG" id="lth:KLTH0F04114g"/>
    <dbReference type="eggNOG" id="ENOG502SEYA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_953368_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKE9"/>
    <dbReference type="OMA" id="HHERIFD"/>
    <dbReference type="OrthoDB" id="4066250at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="246"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="292" mass="33135" checksum="8616A78E2D41487D" modified="2009-07-28" version="1">MAFNSYHRRGSRSAAHATLANQNFDEVEELLSRNVNALDIHILNAPLQHEQDAVSEDSRLFTNEVKSEEFAILSTSSERDANRELELVGDLLGAAAFEKPELEGAAELAVSKSQRIDDWCLQNGLRNQTFQAPANRKIQDIIEAWGVVDDSPPTRMLRPFRQSEPSHKHPENYTAHLLDLMSPPQRLRLRKFVEQMMPLLKRQLTTRFQAYRFKSVPSAFINRDLQLHCSLQGELLLEPQSANDLVGSMNSDHNESSRCSSPSSISKASENVRSWHSWNMVSRSDADHIPSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKG3</accession>
    <name>C5DKG3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">KLTH0F04444p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0F04444g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554401.1">
        <property type="nucleotide sequence ID" value="XM_002554355.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKG3"/>
    <dbReference type="FunCoup" id="C5DKG3">
        <property type="interactions" value="128"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKG3"/>
    <dbReference type="GeneID" id="8292596"/>
    <dbReference type="KEGG" id="lth:KLTH0F04444g"/>
    <dbReference type="eggNOG" id="ENOG502S44N">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027592_4_0_1"/>
    <dbReference type="InParanoid" id="C5DKG3"/>
    <dbReference type="OMA" id="VLVMFPF"/>
    <dbReference type="OrthoDB" id="2874149at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004932">
        <property type="term" value="F:mating-type factor pheromone receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000750">
        <property type="term" value="P:pheromone-dependent signal transduction involved in conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14966">
        <property type="entry name" value="7tmD_STE3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001499">
        <property type="entry name" value="GPCR_STE3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28097">
        <property type="entry name" value="PHEROMONE A FACTOR RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28097:SF1">
        <property type="entry name" value="PHEROMONE A FACTOR RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02076">
        <property type="entry name" value="STE3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00899">
        <property type="entry name" value="GPCRSTE3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0589">Pheromone response</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="77"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="119"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="161"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="209"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="265"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="383"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="434"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="434"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR23964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="469" mass="51724" checksum="CFA702B8AC854D80" modified="2009-07-28" version="1">MSYSSVIIGLGSVALALLFPPLAWHSKTRNTPAIIMIVWLILMDIKLIVDASIWGGSDFAGKWAGYGWCDLTTKLQIGANVGISCTVANIAYNLHAILRADAVLPEPDSRRKICTDLGISLVTPILVMGLTYLVQVFRYGIFRYNGCQSMLSPSWLTVVTYTLWMFIWSFVGFVYAILLLYVFYRKRKDVKDILHCTSSGLNIARFSRLLLFCVLIILVMFPFSLYAFASELQNLTGAYNYQMVHDSSLWSTIMRIDVAKPLYSVWLYILMSYLVFVIFGLGTDAVGMYGSAARVLGLGPLLDKCKQCCRRRRSENTTKVVSYFFAEHDAFKGADGMGSASDGSSTLKPGVGYSDGSSIEMSDSAPSSPSHFIIDYLLPNEKSRREKRRRNGTGGDLESLSYHDHPANPISFEEEVPLSVQRTKSGLVSHVSYRSASEFGNTSGELGTGNSEEGVSPESPHPVFSSSAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKJ1</accession>
    <name>C5DKJ1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">fructose-2,6-bisphosphate 2-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.46</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0F05126g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the phosphoglycerate mutase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.46" evidence="2"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554429.1">
        <property type="nucleotide sequence ID" value="XM_002554383.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKJ1"/>
    <dbReference type="FunCoup" id="C5DKJ1">
        <property type="interactions" value="508"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKJ1"/>
    <dbReference type="GeneID" id="8292627"/>
    <dbReference type="KEGG" id="lth:KLTH0F05126g"/>
    <dbReference type="eggNOG" id="KOG0234">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006383_1_1_1"/>
    <dbReference type="InParanoid" id="C5DKJ1"/>
    <dbReference type="OMA" id="RCLMGYF"/>
    <dbReference type="OrthoDB" id="267323at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003873">
        <property type="term" value="F:6-phosphofructo-2-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004331">
        <property type="term" value="F:fructose-2,6-bisphosphate 2-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006003">
        <property type="term" value="P:fructose 2,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006000">
        <property type="term" value="P:fructose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1240:FF:000005">
        <property type="entry name" value="GpmB, Fructose-2,6-bisphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000644">
        <property type="entry name" value="GpmB, Fructose-2,6-bisphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003094">
        <property type="entry name" value="6Pfruct_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013079">
        <property type="entry name" value="6Phosfructo_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001345">
        <property type="entry name" value="PG/BPGM_mutase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606:SF44">
        <property type="entry name" value="6-PHOSPHOFRUCTO 2-KINASE_FRUCTOSE 2,6-BISPHOSPHATASE LONG FORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606">
        <property type="entry name" value="6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01591">
        <property type="entry name" value="6PF2K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000709">
        <property type="entry name" value="6PFK_2-Ptase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00991">
        <property type="entry name" value="6PFRUCTKNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00175">
        <property type="entry name" value="PG_MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="6-phosphofructo-2-kinase" evidence="8">
        <location>
            <begin position="1"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="403"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="212"/>
            <end position="219"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR23992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="430" mass="49775" checksum="A4D604C4454EC761" modified="2009-07-28" version="1">MVGLPARGKSFISQKIVRYLSWLSIKSKCFNVGSYRRQGAPRPSAGFFDASNKEAYRIREQAVAQAVEDMMNWFSDEEGVVAILDATNSTKERRKWILRLCRENSIEPMFLESWCDDKDLVLRNIADVKTSSPDYKGIDPDVATADFLERIANYEQVYQPLDTTDDRDLTFVRMINIAQQVIINKVETYLESRIVFYVMNLHIKPRYIWLSRHGESIYNIEKKIGGDSSLSERGLKYAKKLPELVRESAGDRDLTVWTSTLIRTQETAQHLPYKQLQWKALDELDAGECDGMTYEEIEEKFPEDFKARDEDKYEYRYRGGESYRDVVIRLEPIIMELERQENILIITHQAVLRCIYAYFMNVPQEESPWMSIPLHTLIRLEPRAYATHVTKIKADIPAVSTYKGKGTSRVGESESDNDKSLHVLSSLNSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKN2</accession>
    <name>C5DKN2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0F06072p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0F06072g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554470.1">
        <property type="nucleotide sequence ID" value="XM_002554424.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKN2"/>
    <dbReference type="FunCoup" id="C5DKN2">
        <property type="interactions" value="45"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKN2"/>
    <dbReference type="GeneID" id="8292668"/>
    <dbReference type="KEGG" id="lth:KLTH0F06072g"/>
    <dbReference type="eggNOG" id="ENOG502QU4J">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042692_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKN2"/>
    <dbReference type="OMA" id="RIHLVHD"/>
    <dbReference type="OrthoDB" id="5594977at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018814">
        <property type="entry name" value="DUF5427"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28265">
        <property type="entry name" value="MAINTENANCE OF TELOMERE CAPPING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28265:SF1">
        <property type="entry name" value="MAINTENANCE OF TELOMERE CAPPING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10310">
        <property type="entry name" value="DUF5427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="419"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="101"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="419"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="478" mass="52081" checksum="E48584657832EEE1" modified="2009-07-28" version="1">MKELVRNTYPSATRAAMSGRNSTEADDVLEFLESLPESSKTSGGSGAENSGKEPKKDTEILDFLDELEQSNLSLKKDKKAGQAEENAQEGEGDSVAQTSTASKEPAAAEKPAAAEKPVAAEEPAAVEDPASSKASQPKETAASVSQADAEIIQSEPETTSDPITSFSNWWSSSGSATVNSFFSKTAEQATHLKDRLAQEQHGIASKLQGTSLGSKINTATLSTLAKNLSRIVVGDTDEVLRIHLVHDLVNYPLLSYHVEQQFDNVLSSQVQSGVRIFVDEWDHPIEGNDRVQDGKRHLNMFTGKVSDGEKLAFANLNNAIKLFTEAKDELAKQRFETVSAEEGEKELAISDVFISILPISVPEKHKNPEIATTDSSHPGNFAFTIVLKDISNDFTSITRSQGFPQRWADWLEGTSELKSKQDAIQDRESKRDAVERTDSADDAEGIDPSDWVKEWVEDGLSLAFGVVAQNYVIDRMGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKQ4</accession>
    <name>C5DKQ4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glycerol-3-phosphate dehydrogenase [NAD(+)]</fullName>
            <ecNumber evidence="6">1.1.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0F06622g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4 6">
            <text>sn-glycerol 3-phosphate + NAD(+) = dihydroxyacetone phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11092"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.8" evidence="6"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554492.1">
        <property type="nucleotide sequence ID" value="XM_002554446.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKQ4"/>
    <dbReference type="FunCoup" id="C5DKQ4">
        <property type="interactions" value="639"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKQ4"/>
    <dbReference type="GeneID" id="8292693"/>
    <dbReference type="KEGG" id="lth:KLTH0F06622g"/>
    <dbReference type="eggNOG" id="KOG2711">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033449_2_3_1"/>
    <dbReference type="InParanoid" id="C5DKQ4"/>
    <dbReference type="OMA" id="NRMFGNM"/>
    <dbReference type="OrthoDB" id="10263760at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141152">
        <property type="term" value="F:glycerol-3-phosphate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046168">
        <property type="term" value="P:glycerol-3-phosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.10:FF:000004">
        <property type="entry name" value="Glycerol-3-phosphate dehydrogenase [NAD(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000294">
        <property type="entry name" value="Glycerol-3-phosphate dehydrogenase [NAD(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006168">
        <property type="entry name" value="G3P_DH_NAD-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006109">
        <property type="entry name" value="G3P_DH_NAD-dep_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017751">
        <property type="entry name" value="G3P_DH_NAD-dep_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011128">
        <property type="entry name" value="G3P_DH_NAD-dep_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03376">
        <property type="entry name" value="glycerol3P_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11728">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11728:SF8">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE DEHYDROGENASE [NAD(+)]-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07479">
        <property type="entry name" value="NAD_Gly3P_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01210">
        <property type="entry name" value="NAD_Gly3P_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00077">
        <property type="entry name" value="GPDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00957">
        <property type="entry name" value="NAD_G3PDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0520">NAD</keyword>
    <keyword evidence="2 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycerol-3-phosphate dehydrogenase NAD-dependent N-terminal" evidence="7">
        <location>
            <begin position="66"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="Glycerol-3-phosphate dehydrogenase NAD-dependent C-terminal" evidence="8">
        <location>
            <begin position="264"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR24055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="430" mass="47425" checksum="18646E8AF8E35E20" modified="2009-07-28" version="1">MFSISRITRTSSFTTQFRALYRFKHSARKLQSIPFSIYKKMSAADRLNQTHDILSESVQAVENPFKVTVIGSGNWGTTISKVVAENAALRPHLFVKRVDMWVFEETVDGQKLTEIINTKHQNVKYLPNIDLPENLVANPDLVSAVKDADILVFNIPHQFLPRIVSQLQGNIKKDARAISCLKGFDVSKDGVKLLSTYVTEKLGITCGALSGANLAPEVAKENWSETTVAYELPKDFKGEGKDVDHAVLKALFHRPYFHVNVIDDVAGISVAGALKNVVALGCGFVEGLGWGNNASAAIQRVGLGEIIKFGQMFFPDSRVETYYQESAGVADLITTCSGGRNVRVATHMAKTGKSAEECEKELLNGQSAQGIHTCKEVHEWLAECGKTDEFVLFEAVYQIVYENAPMDTLPDMIEDLQGVNIPKEEGEHSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKR9</accession>
    <name>C5DKR9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NADH:ubiquinone reductase (non-electrogenic)</fullName>
            <ecNumber evidence="3">1.6.5.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0F06974g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a quinone + NADH + H(+) = a quinol + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:46160"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.6.5.9"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a ubiquinone + NADH + H(+) = a ubiquinol + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:23152"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NADH dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.6.5.9" evidence="3"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554507.1">
        <property type="nucleotide sequence ID" value="XM_002554461.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKR9"/>
    <dbReference type="FunCoup" id="C5DKR9">
        <property type="interactions" value="184"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKR9"/>
    <dbReference type="GeneID" id="8292709"/>
    <dbReference type="KEGG" id="lth:KLTH0F06974g"/>
    <dbReference type="eggNOG" id="KOG2495">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021377_1_0_1"/>
    <dbReference type="InParanoid" id="C5DKR9"/>
    <dbReference type="OMA" id="EPAEIKY"/>
    <dbReference type="OrthoDB" id="3244603at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050136">
        <property type="term" value="F:NADH dehydrogenase (quinone) (non-electrogenic) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015980">
        <property type="term" value="P:energy derivation by oxidation of organic compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.100:FF:000007">
        <property type="entry name" value="Rotenone-insensitive NADH-ubiquinone oxidoreductase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045024">
        <property type="entry name" value="NDH-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054585">
        <property type="entry name" value="NDH2-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43706">
        <property type="entry name" value="NADH DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43706:SF10">
        <property type="entry name" value="ROTENONE-INSENSITIVE NADH-UBIQUINONE OXIDOREDUCTASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22366">
        <property type="entry name" value="NDH2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="12">
        <location>
            <begin position="58"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="domain" description="External alternative NADH-ubiquinone oxidoreductase-like C-terminal" evidence="13">
        <location>
            <begin position="446"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF22366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR24070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="516" mass="56840" checksum="0D422B9DF664B435" modified="2009-07-28" version="1">MLSQFASRNAGRASALNAARRLARFASDSARVDGASGGGVSSFKTNKIIENTLGNKPNVVILGSGWGAISLLQHIDSRSYNVTIVSPRNYFLFTPLLPSTPVGTVDEKSIIEPVVNFALKKKGNVSYYEALATAINPERNTVSIKAVSTVAQLAQPDNHLGLNQHDEAEIKYDYLVSAVGAEPNTFGIPGVEKHGNFLKEIPHSLEIRKRFLSNIEKANLLPKGDPERRRLLTIVVVGGGPTGVETAGELQDYIDQDLKRFMPSVAEEVQIHLVEALPVVLNMFERKLTSYAQDVLQKTRIKVHLRTAVARVEEDHLVAKTKAEDGATTEQTIPYGTLIWATGNKALSIVTDLFKKIPAQNDSKRGLAVNQNLLVKGSNNIFAVGDNAFSGLPPTAQVAHQQAEYLAKVLSKMAKSPNFHAELAQRKEKVDLFFEQKGIKPFNYTHYGALAYLGAEKAIANITYGKRSFYTGGGVLTFYVWRVTYAMMILSARSRFKVIADWLKLAFFKRDCFKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKU4</accession>
    <name>C5DKU4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0F07590p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0F07590g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eIF-2-beta/eIF-5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554532.1">
        <property type="nucleotide sequence ID" value="XM_002554486.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKU4"/>
    <dbReference type="FunCoup" id="C5DKU4">
        <property type="interactions" value="1282"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKU4"/>
    <dbReference type="GeneID" id="8292737"/>
    <dbReference type="KEGG" id="lth:KLTH0F07590g"/>
    <dbReference type="eggNOG" id="KOG2767">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026663_1_0_1"/>
    <dbReference type="InParanoid" id="C5DKU4"/>
    <dbReference type="OMA" id="YRYKMEK"/>
    <dbReference type="OrthoDB" id="10250831at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071074">
        <property type="term" value="F:eukaryotic initiation factor eIF2 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005092">
        <property type="term" value="F:GDP-dissociation inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11561">
        <property type="entry name" value="W2_eIF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000031">
        <property type="entry name" value="Eukaryotic translation initiation factor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.350:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.170:FF:000002">
        <property type="entry name" value="Eukaryotic translation initiation factor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045196">
        <property type="entry name" value="IF2/IF5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002735">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016189">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016190">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003307">
        <property type="entry name" value="W2_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001:SF7">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01873">
        <property type="entry name" value="eIF-5_eIF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02020">
        <property type="entry name" value="W2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00653">
        <property type="entry name" value="eIF2B_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00515">
        <property type="entry name" value="eIF5C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100966">
        <property type="entry name" value="Translation initiation factor 2 beta, aIF2beta, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75689">
        <property type="entry name" value="Zinc-binding domain of translation initiation factor 2 beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51363">
        <property type="entry name" value="W2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="W2" evidence="7">
        <location>
            <begin position="240"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="168"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR24095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="399" mass="44538" checksum="EF5A1DCA636FAF65" modified="2009-07-28" version="1">MSINICRDNNDPFYRYKMPPLQAKVEGRGNGIKTAVLNVADVARALNRPSPYIVKYFGFELGAQTSISVDKDRYLVNGSHEPAKLQDVLDGFINKFVLCGSCKNPETEIVITKNDDLVRDCKACGKRTPMDLRHKLSSFILKNPPESMESNKKRKAATASANIRGGGVSISDIAQGKSQSSAQAKAETEGDSDDDELARQINAAASSLQEIEVNNDDWAVDMSEEAIRARAKELRAANGEDEEISKLEEYGEWILSQDEVPSDVELYKTAVKFELLQEPKIACVLAQALFSEDIVEEVSEHTAFFKKLFISEEFENQFLGGIERFLGLEHKELIPALPKILVQLYNNDIVSEEVIVKFGTKCSKKFVPKDVSKKVRRAAKPFITWLETAESEDEDDDSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLA3</accession>
    <name>C5DLA3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0F11352p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0F11352g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CCZ1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554691.1">
        <property type="nucleotide sequence ID" value="XM_002554645.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLA3"/>
    <dbReference type="FunCoup" id="C5DLA3">
        <property type="interactions" value="118"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLA3"/>
    <dbReference type="GeneID" id="8292904"/>
    <dbReference type="KEGG" id="lth:KLTH0F11352g"/>
    <dbReference type="eggNOG" id="ENOG502QSQV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_418686_0_0_1"/>
    <dbReference type="InParanoid" id="C5DLA3"/>
    <dbReference type="OMA" id="YNCLFWY"/>
    <dbReference type="OrthoDB" id="240546at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035658">
        <property type="term" value="C:Mon1-Ccz1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013176">
        <property type="entry name" value="Ccz1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043987">
        <property type="entry name" value="CCZ1/INTU/HSP4_longin_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13056">
        <property type="entry name" value="VACUOLAR FUSION PROTEIN CCZ1 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13056:SF0">
        <property type="entry name" value="VACUOLAR FUSION PROTEIN CCZ1 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19031">
        <property type="entry name" value="Intu_longin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCZ1/INTU/HSP4 first Longin" evidence="3">
        <location>
            <begin position="2"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="261"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR24254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="631" mass="71962" checksum="4030C4BC8F4922AB" modified="2009-07-28" version="1">MLDSITVFNPSYSTNESDSDKQLLLYHSFQGEQSYSLNEKLSQIGIIQAVWSFSQSFGDFKGVHQNVVELDQQIICTVLVEDDFFVSLAITAEHTSIPASYVFSHLRHCYDFFVLQFGLWRDFHDRRALTDRLNEVLVSYWSELNLMPQYLQFKGFLSLWPQAYKVAELPGLEDSSEPSWESDLKQQILLDETSFLGLKDVLVYNLPKPTSRKGYKSYGLVRNFAPNCDSLPQVSNWIEHMDTIYTRLSSHVIAGNVQYKENPEDEEPAENNAEHTTAGESLRNHSRKLYHNVTLPISFAYDAVQEVGNMAGISSSVSLLTDFMPNISSWNPWGSSAKKEKKARSEFLISPLSASFLPDSYKVKKLRLSFDQNAKEYNCLFWFYNEILVILVFEQDFMKIWEHEFLSDLHFKLQSCISKLSSEHLNTVPHNAEKFCYAVVSKKTSQIKSSFPLYKAPKVSETASPLELVVNGLDEFLNSGANRKTSVSTISANDVQLADEERSARQSWGLGLMGGFFKKEQSGKTPQVQVDTFLDCIPEEKLRDLHLDVTTFVNTLSTSKRADDLKEEKLIKLNNGVLCFIREDSEEIVVLAENWFNKKASKSKASTPSRDGLVEYMGKEASEWWSNRTHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLC8</accession>
    <name>C5DLC8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0F11924p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0F11924g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RNase PH family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554716.1">
        <property type="nucleotide sequence ID" value="XM_002554670.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLC8"/>
    <dbReference type="FunCoup" id="C5DLC8">
        <property type="interactions" value="294"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLC8"/>
    <dbReference type="GeneID" id="8292930"/>
    <dbReference type="KEGG" id="lth:KLTH0F11924g"/>
    <dbReference type="eggNOG" id="KOG1069">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063514_2_1_1"/>
    <dbReference type="InParanoid" id="C5DLC8"/>
    <dbReference type="OMA" id="CIINEQG"/>
    <dbReference type="OrthoDB" id="27298at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071028">
        <property type="term" value="P:nuclear mRNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071051">
        <property type="term" value="P:poly(A)-dependent snoRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11372">
        <property type="entry name" value="RNase_PH_RRP46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.70:FF:000027">
        <property type="entry name" value="Exosome complex component RRP46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015847">
        <property type="entry name" value="ExoRNase_PH_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050080">
        <property type="entry name" value="RNase_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953:SF1">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03725">
        <property type="entry name" value="RNase_PH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="Ribonuclease PH domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0271">Exosome</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="6">
        <location>
            <begin position="2"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="7">
        <location>
            <begin position="133"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR24279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="224" mass="24583" checksum="CC059145788BF109" modified="2009-07-28" version="1">MISAQTGVLDQVDGSCKLQCGETTVICSVTGPIEPKARQELPSQLALEVVVRPCKGVPNTREKLLEDQIRGVVTPVLAKYLYPRQLCQICFQILESGEPEDEYNVKELNSCINAAYLALIDSGVALQSSFSSVCVSVLGSNNEILVNPTQEQLIKSDSTHILALEVGSGGKKIQNVLLIESNGDFTEEIMFNVLSRAEKACVEVALELRKAVEDKIKRDFIWKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLW1</accession>
    <name>C5DLW1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein bL33m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0G03982g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial ribosomal protein bL33 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555209.1">
        <property type="nucleotide sequence ID" value="XM_002555163.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLW1"/>
    <dbReference type="FunCoup" id="C5DLW1">
        <property type="interactions" value="288"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLW1"/>
    <dbReference type="GeneID" id="8293475"/>
    <dbReference type="KEGG" id="lth:KLTH0G03982g"/>
    <dbReference type="eggNOG" id="KOG3505">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_190949_1_2_1"/>
    <dbReference type="InParanoid" id="C5DLW1"/>
    <dbReference type="OMA" id="TQVRYDP"/>
    <dbReference type="OrthoDB" id="275534at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.120">
        <property type="entry name" value="Ribosomal protein L33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052008">
        <property type="entry name" value="Mitoribosomal_protein_bL33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038584">
        <property type="entry name" value="Ribosomal_bL33_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47037">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L33, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47037:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL33M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR24772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="70" mass="8027" checksum="F394747567C006A5" modified="2009-07-28" version="1">MAKQKSKNSVIKLLSTAATGFARQITITRGAPLVTQVRYDPVAKRHVLFKEAKKRKVAERKPLDFRRTFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5DM39</accession>
    <name>C5DM39_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0G05742p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0G05742g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555287.1">
        <property type="nucleotide sequence ID" value="XM_002555241.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DM39"/>
    <dbReference type="STRING" id="559295.C5DM39"/>
    <dbReference type="GeneID" id="8293554"/>
    <dbReference type="KEGG" id="lth:KLTH0G05742g"/>
    <dbReference type="eggNOG" id="ENOG502S32U">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_535347_0_0_1"/>
    <dbReference type="InParanoid" id="C5DM39"/>
    <dbReference type="OMA" id="ESRVWYR"/>
    <dbReference type="OrthoDB" id="548867at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000747">
        <property type="term" value="P:conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051228">
        <property type="entry name" value="NADPH_Oxidase/PX-Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15706:SF2">
        <property type="entry name" value="SH3 AND PX DOMAIN-CONTAINING PROTEIN 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15706">
        <property type="entry name" value="SH3 MULTIPLE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PX" evidence="3">
        <location>
            <begin position="182"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="138"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR24850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="509" mass="56436" checksum="6F7C02C3C9A988EE" modified="2009-07-28" version="1">MMKTADEVKFENNEAANRSKSRLAGILRSFSSAGSYSKRKVPTISNPRAEFPFAKPNLSAKRDRVFLDVLQDYLPNEPAQSAGSAARSRFLNIYRGEIVQLLEPAPNNLVKVRLVNRLGQGLVPARCVAVNVELTPSKMAQQGSQEDLEVSEPGSPTLSQKLESEPETESESESPKPQAPASGLNAVEKCQVVGIHMWENRILYRIDCIMRSGHRRALSRFYQDFYSLQLQLRDRMVEEGIEDPQEVLPGLPSPSRKVDDSKVTNRLDVFNEYLHQIISSEHISQSIKRDVVLDKWLSLRPGDLVKTQRGSLYKVNEAASESREDFTWESLDPSTSIDALDRDLSPLPTDWNLGALGGPKAKPPIPRAPSLTDMPVRRKPLMKLSADSAPTTPITNTLAKCLTPTSAPPSPEQVGTDHVKVKILHKDDCYAAKCTLSELESYEKLDSLCRSKLKSCLTSADCPLTISYVNDKKEPLELSREIFDDVLPKLVLAASGSIVTKLTFRVVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>C5DM42</accession>
    <name>C5DM42_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">KLTH0G05808p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0G05808g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555290.1">
        <property type="nucleotide sequence ID" value="XM_002555244.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DM42"/>
    <dbReference type="FunCoup" id="C5DM42">
        <property type="interactions" value="431"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DM42"/>
    <dbReference type="GeneID" id="8293557"/>
    <dbReference type="KEGG" id="lth:KLTH0G05808g"/>
    <dbReference type="eggNOG" id="KOG0756">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015166_5_0_1"/>
    <dbReference type="InParanoid" id="C5DM42"/>
    <dbReference type="OMA" id="KDWYKGG"/>
    <dbReference type="OrthoDB" id="204711at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005469">
        <property type="term" value="F:succinate:fumarate antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049563">
        <property type="entry name" value="TXTP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45788">
        <property type="entry name" value="SUCCINATE/FUMARATE MITOCHONDRIAL TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45788:SF2">
        <property type="entry name" value="SUCCINATE_FUMARATE MITOCHONDRIAL TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="8"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="113"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="239"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR24853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="341" mass="36571" checksum="9AC908257F1DF8DA" modified="2009-07-28" version="1">MSSRKNSSHPAINLVAGGTAGLFEALCCHPLDTIKVRMQIYSRAQEIAEHARRAPGFVSTARDIARQEGFLAFYKGLGAVVIGIIPKMAIRFTSYEFFRTLLADPGSGAVSTGNTFLAGVGAGITEAVLVVNPMEVVKIRLQAQHVRYVPLAAELAGAASPHTASLAGASTATANVPAAPKYRNAIQAAFVIVKEEGPRALYRGVSLTAARQATNQGANFTVYSTLKTRLQEYHQTDMLPSWETSLIGLLSGAVGPFSNAPLDTIKTRLQKDKSVSKDSGWARIVAIGRQLIREEGFRALYKGITPRVMRVAPGQAVTFTVYELIREQLEGLAASRKLATS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DM43</accession>
    <name>C5DM43_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0G05830p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0G05830g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL43 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555291.1">
        <property type="nucleotide sequence ID" value="XM_002555245.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DM43"/>
    <dbReference type="FunCoup" id="C5DM43">
        <property type="interactions" value="1116"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DM43"/>
    <dbReference type="GeneID" id="8293558"/>
    <dbReference type="KEGG" id="lth:KLTH0G05830g"/>
    <dbReference type="eggNOG" id="KOG0402">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_141199_1_0_1"/>
    <dbReference type="InParanoid" id="C5DM43"/>
    <dbReference type="OMA" id="GPRYGRK"/>
    <dbReference type="OrthoDB" id="10258345at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.30:FF:000002">
        <property type="entry name" value="60S ribosomal protein L37a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00327">
        <property type="entry name" value="Ribosomal_eL43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011331">
        <property type="entry name" value="Ribosomal_eL37/eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002674">
        <property type="entry name" value="Ribosomal_eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050522">
        <property type="entry name" value="Ribosomal_protein_eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00280">
        <property type="entry name" value="eL43_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003058">
        <property type="entry name" value="PRK03976.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48129">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L37A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48129:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01780">
        <property type="entry name" value="Ribosomal_L37ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR24854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="92" mass="10049" checksum="E20DC74E78B11730" modified="2009-07-28" version="1">MAKRTKKVGITGKYGVRYGSSLRRQVKKLEIQQHSRYDCSFCGKKTVKRGAAGIWTCSSCKKTVAGGAYTVSTAAAATVRSTIRRLRDMAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMB9</accession>
    <name>C5DMB9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0G07568p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0G07568g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LTV1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555367.1">
        <property type="nucleotide sequence ID" value="XM_002555321.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMB9"/>
    <dbReference type="FunCoup" id="C5DMB9">
        <property type="interactions" value="518"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMB9"/>
    <dbReference type="GeneID" id="8293637"/>
    <dbReference type="KEGG" id="lth:KLTH0G07568g"/>
    <dbReference type="eggNOG" id="KOG2637">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028555_1_0_1"/>
    <dbReference type="InParanoid" id="C5DMB9"/>
    <dbReference type="OMA" id="TAQHFTL"/>
    <dbReference type="OrthoDB" id="5852896at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000056">
        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007307">
        <property type="entry name" value="Ltv1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21531">
        <property type="entry name" value="LOW-TEMPERATURE VIABILITY PROTEIN LTV1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21531:SF0">
        <property type="entry name" value="PROTEIN LTV1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04180">
        <property type="entry name" value="LTV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="291"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="449"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="300"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="340"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR24930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="483" mass="54747" checksum="4983631836035540" modified="2009-07-28" version="1">MSSKGAFNRNNAQKFAVVHRPHDDPHFHDPEVGGHVLVPVNDPKANRAQVKAKSPLTRNVVGQSARQSRKPTNDHVGEAALYGIGFDDSNYDYTKHLKPIGTDPENSFLINASKPAEKPQTAKKLNVEDLFVEPEYKESSKKNESMFQRGVAKREYLIQQQDIEEDLRGFKPDLNPALREVLEALEDEAYVVNKDIVVEKPKKKKTAAEETESIFGAEETGDEDIFAELLMSGQAGDADEFEQEFDEWDMENLEDYEDEHYNNELQQFQNIENLEDLQNIDYQADVARFKQHKSRAGPEDGWDSVDEFEDDGASLSAASIEEEEQDQLGDLPDLSASKNKNAKQKKRKDRRKKGAMSDVSGFSMSSSAIARTEVLTVLDDKYDRIISGYENYEQEQEEEEEQSYQPFDMANERADFESMLDDFLDNYELESGGRKLVKKSKEIDRLKNAADEVSKGKLSQRRLKERSKGGVDGITGSLSSLKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMT9</accession>
    <name>C5DMT9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0G11572p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0G11572g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555537.1">
        <property type="nucleotide sequence ID" value="XM_002555491.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMT9"/>
    <dbReference type="GeneID" id="8293816"/>
    <dbReference type="KEGG" id="lth:KLTH0G11572g"/>
    <dbReference type="HOGENOM" id="CLU_882987_0_0_1"/>
    <dbReference type="InParanoid" id="C5DMT9"/>
    <dbReference type="OMA" id="YLRIMCM"/>
    <dbReference type="OrthoDB" id="4036071at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="160"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="275"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="304"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR25100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="315" mass="35630" checksum="A0D9AD47EBB784DA" modified="2009-07-28" version="1">MNESYDWSEEAQKLIKFRDQKVLEPAQNDLLCKRLVIERRIKEAAEEHLKNENAFQLGRLDKVSPEYLRIMCMDYEDLKTAGVAYNPAKIRKEGASSVLRSSDQDELESPESGAATMETLVSKELENDKDSGNILHVSRGQEMVLNARKNTALVKSMLDTQSASSNGIHISKKREVEILSDGSESDSDDSRSKSKRRQVGTHPDAFAHEAQVQFSPDNQAQLFQYERSLSGNAVPTYGYMQQQQVLNMTQQITSRSPPQIHAHSHQQYQIPPRTVKKTRTGTLPPRQLPEAEQSSKNKTPKALRKMENVARKHGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMV6</accession>
    <name>C5DMV6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0G11990p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0G11990g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555554.1">
        <property type="nucleotide sequence ID" value="XM_002555508.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMV6"/>
    <dbReference type="GeneID" id="8293835"/>
    <dbReference type="KEGG" id="lth:KLTH0G11990g"/>
    <dbReference type="eggNOG" id="KOG3511">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_450604_0_0_1"/>
    <dbReference type="InParanoid" id="C5DMV6"/>
    <dbReference type="OrthoDB" id="4066654at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031778">
        <property type="entry name" value="Sortilin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050310">
        <property type="entry name" value="VPS10-sortilin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12106">
        <property type="entry name" value="SORTILIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12106:SF27">
        <property type="entry name" value="SORTILIN-RELATED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15902">
        <property type="entry name" value="Sortilin-Vps10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110296">
        <property type="entry name" value="Oligoxyloglucan reducing end-specific cellobiohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Sortilin N-terminal" evidence="2">
        <location>
            <begin position="95"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF15902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR25117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="611" mass="67962" checksum="E276D5C1C57480F9" modified="2009-07-28" version="1">MKQDLHAICWNARALALGNVLAHSGSGRTGPSYYFPYSDQATGPSCAFAGSSTLLTLQGGLLRITNATHRNWTIVDQVGHNISSFELDENNPGFRAFAFDGTYARVFQTMDQGRTWTQMTNKESFWDSAEDKNNPKVRLNFHVQTNAFALEDILVTVVRCESITKEVSSCEASTSVFVSVDGKHFRRRAKIKAPDGCPISYRCYFSGSDAGARVICELRVLLPSPKGGFLGQDVGLFYSDNHGKTFVALEEFKRQMVWCLQAIGNFSTVITVKPKKWYRTEPSFELWMSRDGSRFERVDQATSRRMPMVESLDHSFKRQLVRLRSVIDGQLTETILVANSHGPYLKYLVRFRPNTQTASLINGLSDSVTAILHPLKTFPHEMSVGLERRAISTSDASRSFRLLNLHGPARELVCNPKDNDDCYFNLLPPEKSLLHEFPGAVALVGTVAMVGGPSRSKPEPITFVTRDSGRTWHKLLDFPALVAFGDHDNVVVAVPSRTDSESPLLYYSTDRGNSWSLHRFEKPIKVLSISRASADISSAIFIMSAKSAEDGHRRYAIDFSDVIANAQQLNSYSGEASGSISEGQKVTKTRDPVRAFRATTSQSTVTHREPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNA2</accession>
    <name>C5DNA2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0G15334p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0G15334g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555700.1">
        <property type="nucleotide sequence ID" value="XM_002555654.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNA2"/>
    <dbReference type="GeneID" id="8293985"/>
    <dbReference type="KEGG" id="lth:KLTH0G15334g"/>
    <dbReference type="HOGENOM" id="CLU_468560_0_0_1"/>
    <dbReference type="InParanoid" id="C5DNA2"/>
    <dbReference type="OMA" id="ITHETNR"/>
    <dbReference type="OrthoDB" id="4035708at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="560"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="263"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="329"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="411"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="561"/>
            <end position="570"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR25263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="582" mass="67222" checksum="7A5DEBE79BD69FA7" modified="2009-07-28" version="1">MNSRSKCGPRFATMEMEADFSSQFILEGSGHPNSNSLPNALLRKDLSLKQWEDTNETEMDFDLYSIDRDNDLRPETYVSDVYEVRNTNPIVEEFVTEQMRVKGRPSSLLRFGCNQTKLFDMHASIGNSHIRKPSGAIKPESILIVKDQEPRYLEEDSHSDWDQDIPIDNPVVLEGFLRCWEACGIPDFEFDAEDMIMTVQKLSYGILQALANQGTVNRLVKAQQFSEITEDFEECWIDLLEALELEDTVKCMEADLAHQTSITAEKEKRIQSLENQVRGLERQLETQVELKQENKDLRAAVEHNTKMFQELNEKYRDVSLKLNVVQMNNDEVSIKHDVLMNENAELRHEIRTCRGTVKDRESQIKQLERTMSNDSREAKKELETSKTQCNQMAFEYKNAEMNWKHRQSMLLSDLEEKSALAEAFNQELERAKNEIEGFKNQNIVSEASLNAAKEYIEELELQNKELNQRISKLCLQVEGLVEFKNKAKAKVALLKDAKRESKQQNKKNARLIAALESRVEELATLSEVKENDNKQLFQELQQYKKSLHGAKVQTPSVLSPLKHKNAHNRRSLQLSDRRIGAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNG4</accession>
    <name>C5DNG4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Probable electron transfer flavoprotein subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0G16786g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7 8">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="7 8">Binds 1 FAD per dimer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 7">Heterodimer of an alpha and a beta subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the ETF alpha-subunit/FixB family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555762.1">
        <property type="nucleotide sequence ID" value="XM_002555716.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNG4"/>
    <dbReference type="FunCoup" id="C5DNG4">
        <property type="interactions" value="537"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNG4"/>
    <dbReference type="GeneID" id="8294051"/>
    <dbReference type="KEGG" id="lth:KLTH0G16786g"/>
    <dbReference type="eggNOG" id="KOG3954">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034178_0_1_1"/>
    <dbReference type="InParanoid" id="C5DNG4"/>
    <dbReference type="OMA" id="WRPYAEQ"/>
    <dbReference type="OrthoDB" id="1715808at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01715">
        <property type="entry name" value="ETF_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1220:FF:000001">
        <property type="entry name" value="Electron transfer flavoprotein, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014730">
        <property type="entry name" value="ETF_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001308">
        <property type="entry name" value="ETF_a/FixB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033947">
        <property type="entry name" value="ETF_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014731">
        <property type="entry name" value="ETF_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43153">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43153:SF1">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01012">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00766">
        <property type="entry name" value="ETF_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000089">
        <property type="entry name" value="Electra_flavoP_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00893">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5 7 8" id="KW-0274">FAD</keyword>
    <keyword evidence="4 7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Electron transfer flavoprotein alpha/beta-subunit N-terminal" evidence="9">
        <location>
            <begin position="25"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="257"/>
            <end position="258"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="271"/>
            <end position="275"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="288"/>
            <end position="295"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000089-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR25325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="341" mass="35793" checksum="D97ECF007C5C311A" modified="2009-07-28" version="1">MFSVGVRSCVGPVVRRPANFRLASTLVFLEANKDGKVAQSSLSALSAAEQLGNPIKALLAGSNSTEAAKQLKLTVGASLVDQIIVANNEVYDNYLPEAVTPLCLELLKEKEFSHFVVASSAVGKNLLPRVGALLDYQPICDVTKIVDPTTFVRPIYAGNALATVQCTQEKKLVSVRASSFPACSAGSFDAPTLEAPQVEIAELGVAWESENLVKSARPDLGSAAKVVSGGRGLKNKETFDKLITPLADSLSAAIGATRAAVDSGFCENSLQIGQTGKVIAPDLYIGVGVSGAIQHLAGMKDSKVIVAINNDQEAPIFQVADFGLVGDLNEIVPELTKKLTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNG8</accession>
    <name>C5DNG8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0G16874p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0G16874g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555766.1">
        <property type="nucleotide sequence ID" value="XM_002555720.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNG8"/>
    <dbReference type="FunCoup" id="C5DNG8">
        <property type="interactions" value="80"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNG8"/>
    <dbReference type="GeneID" id="8294055"/>
    <dbReference type="KEGG" id="lth:KLTH0G16874g"/>
    <dbReference type="eggNOG" id="ENOG502S5IA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103442_0_0_1"/>
    <dbReference type="InParanoid" id="C5DNG8"/>
    <dbReference type="OMA" id="HMYLLVN"/>
    <dbReference type="OrthoDB" id="4067912at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR25329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="227" mass="26533" checksum="D36F9E6A31A52D5D" modified="2009-07-28" version="1">MTDQQVLFMSPQAENLEYLYSLMNKVSQQMSQNKLKRAELLREIDVLVNEVNRTSIRPRPNNPNIAVISNFLKQRNVHIENPTEAQVEDERELQCLRQQNAALKSMLKDKCSTNNETLALLKVHETYLSDVVSLLRADVLTYHQELIRMCRSLYQEQVCSLEDNEFKEYMENISDFQELLDVSEIFRLLLRLTTQTGRTPKIAVGSKYCNILHSTYLRPNNEADTYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNK8</accession>
    <name>C5DNK8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0G17864p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0G17864g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555806.1">
        <property type="nucleotide sequence ID" value="XM_002555760.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNK8"/>
    <dbReference type="STRING" id="559295.C5DNK8"/>
    <dbReference type="GeneID" id="8294100"/>
    <dbReference type="KEGG" id="lth:KLTH0G17864g"/>
    <dbReference type="eggNOG" id="ENOG502QW4F">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_550063_0_0_1"/>
    <dbReference type="InParanoid" id="C5DNK8"/>
    <dbReference type="OMA" id="NFITMGI"/>
    <dbReference type="OrthoDB" id="5043642at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021848">
        <property type="entry name" value="HODM_asu-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11927">
        <property type="entry name" value="HODM_asu-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR25369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="496" mass="56575" checksum="BA41794966BEFA01" modified="2009-07-28" version="1">MIDLSLVLGKHVTFPLLCYVSYLWYMDLKAEQRAEQKADDEHRVPARDQPADEPAKDRSESTIGSKWSPIPLMKEQVTPATSEPDYRPWYATKPYPYKPFKSGEYRLNMGVKPIPLSHWLIMENTYKERIEAKWEIIKKNYKDVMFCLDPAMVNSTGSGGAAPVATGDAAFSEGTLVTLEDVEKCLEAVCELYDHVVGYLCQRFPQYFEVVLSPSSESAGVFHSKVMDEFHPVDPRAYLELENDSLAFIQYRCKNADIVPSKVTDGSVKKDSVGYMSLVTTARTRRAHELILAMARMVEEDKMLLLPNSSGQFNNEYILMAGCFAFAAGFNPRQRFLKPLTLVHGPVPDYKKNLQAHMNRFFHTHQPGRLVLRVNFSFQTHTKLYVTDDNKGADGELIFAKTLDQLRGGRDLFYRSERQCLIKLGPSTNAMCFSIKTYLWSMSEQFLTDEYYSQHGVLQDLYDAVAGMQDNISHYKRKPEWGPALLGLLQKRLQAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5E213</accession>
    <name>C5E213_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0H01342p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0H01342g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555936.1">
        <property type="nucleotide sequence ID" value="XM_002555890.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E213"/>
    <dbReference type="FunCoup" id="C5E213">
        <property type="interactions" value="197"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E213"/>
    <dbReference type="GeneID" id="8294249"/>
    <dbReference type="KEGG" id="lth:KLTH0H01342g"/>
    <dbReference type="eggNOG" id="ENOG502QQBW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033072_0_0_1"/>
    <dbReference type="InParanoid" id="C5E213"/>
    <dbReference type="OMA" id="CSEEHWA"/>
    <dbReference type="OrthoDB" id="5282002at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046824">
        <property type="entry name" value="Mss51-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032717">
        <property type="entry name" value="Mss51_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013272">
        <property type="entry name" value="Vps72/YL1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28069">
        <property type="entry name" value="GH20023P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28069:SF1">
        <property type="entry name" value="PROTEIN MSS51, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20179">
        <property type="entry name" value="MSS51_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13824">
        <property type="entry name" value="zf-Mss51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00993">
        <property type="entry name" value="YL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Vps72/YL1 C-terminal" evidence="2">
        <location>
            <begin position="70"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR30074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="422" mass="49208" checksum="B593B3750113EE4D" modified="2009-07-28" version="1">MQRCILSRNTVRSCQHNVQKRHIMGFVRNALGLDPPPSPDEPTPENRFHPWDQSPSPDLRERAATIRALAKCPVTGREINYTCPKSGIPTHYSREAWENDLEYQQSKRPEILKKVNIYEHDLRSGRPFPEFDFPLDQDFDRMVNLTNWDLFFYTRGFYSMDTEFQLAAVTKMLSYPVTIGSVLHQFSPYSLNPKGPVTLEGLKSLAALRYTLYPEQNRRVTSITKNSPLRIFILGARAEAQLPGHVWKQLQFMFPETNIELHFVGPESYYDRNKREYVHSSSPIVRRVDETLSFVYHTDYFHVFHEAQDFFPYDPYTDIFFCFHPGFAAPESRENWMGKTMEALLETKCAVFTTGFNELDLRRDVGTVVGKHGKELDMLMEPVHNAFGSTKWELNDMNPQEVYQFNMFIAGFRGKRYHAIEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2H9</accession>
    <name>C5E2H9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0H05082p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0H05082g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556102.1">
        <property type="nucleotide sequence ID" value="XM_002556056.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2H9"/>
    <dbReference type="FunCoup" id="C5E2H9">
        <property type="interactions" value="180"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2H9"/>
    <dbReference type="GeneID" id="8294416"/>
    <dbReference type="KEGG" id="lth:KLTH0H05082g"/>
    <dbReference type="eggNOG" id="ENOG502QR2V">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023420_0_0_1"/>
    <dbReference type="InParanoid" id="C5E2H9"/>
    <dbReference type="OMA" id="RISHEYT"/>
    <dbReference type="OrthoDB" id="5430106at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031931">
        <property type="term" value="C:TORC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031929">
        <property type="term" value="P:TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018857">
        <property type="entry name" value="TORC1_cplx_su_TCO89"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22794">
        <property type="entry name" value="THAP DOMAIN PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22794:SF2">
        <property type="entry name" value="THAP DOMAIN-CONTAINING PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10452">
        <property type="entry name" value="TCO89"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="168"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="275"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="380"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="434"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="651"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="201"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="225"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="275"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="434"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="463"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="480"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="682"/>
            <end position="703"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR30240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="720" mass="80207" checksum="B9106AA639C975EB" modified="2009-07-28" version="1">MSVAQSRGRSLRLDSEFASAMNNKHTASNSGKKLRQFTTNSRSKSAASFKGLNKLRRVLSHDGALEIEDYAKERPDFMKKSKSSDSLSRKRAISGLSMTALGKVRSHPGHSSASDANGGSGSNSGQHHVGLRPHRGNSVKSVLALQGGDDELYEDHSTTDEEVEYFTDEEHEGQNGGQSLRARQQPRSRSRQKQNTSSNRVYDELQRSASRSSREDQTLRGSVKELSSSLGQGTVDTELLREDSNKTLGNNQLHYGVPSNLRDQDEDHIDNGDHINAGDHINNEDHINNEDHIGNGDHIDAGDHINNEDHIDQGNPDEDTINANSNIEIIANSLKRHGPSTRNDELIYGNDANTENATQNHSLQSTGRFSPVMILSQSTGVEKRFDRPANIAEDPTSQRFNSKDQDSENKVQSNNFNYINSDFTASYASSSISERQPQNVSKPDFSTSISSLSSHLHKPAQVTADNRTTSILHQRAPQASLPTNNGNSSRLEGHSSSANRLDPLSNFNNFSQFLQSETSGAVSRTQQKLWLQRESSIMDLSANNSGADTIFLASNIEVKREFERISREYTNVRRFSNPLNASLNRVVPRSKIEVKKHRSPFSQGDELSKVFKNEFSKDAKTFKEFCRENLNQKLDIDRVLARIWNQESEDFNKESELLGDNEPEGASSMPAGQRTHNRHSLRNNGPNSNTNHLRTVSSLQPTTRAVHRRMESALGQQQRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2S3</accession>
    <name>C5E2S3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0H07282p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0H07282g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556196.1">
        <property type="nucleotide sequence ID" value="XM_002556150.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5E2S3">
        <property type="interactions" value="687"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2S3"/>
    <dbReference type="GeneID" id="8294513"/>
    <dbReference type="KEGG" id="lth:KLTH0H07282g"/>
    <dbReference type="eggNOG" id="KOG1450">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002085_0_0_1"/>
    <dbReference type="InParanoid" id="C5E2S3"/>
    <dbReference type="OMA" id="TLEDDRW"/>
    <dbReference type="OrthoDB" id="79452at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005933">
        <property type="term" value="C:cellular bud"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06224">
        <property type="entry name" value="REM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00159">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000651">
        <property type="entry name" value="Ras-like_Gua-exchang_fac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050729">
        <property type="entry name" value="Rho-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23176:SF96">
        <property type="entry name" value="GTPASE-ACTIVATING PROTEIN BEM2_IPL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23176">
        <property type="entry name" value="RHO/RAC/CDC GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00618">
        <property type="entry name" value="RasGEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00229">
        <property type="entry name" value="RasGEFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ras-GEF" evidence="4">
        <location>
            <begin position="436"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="5">
        <location>
            <begin position="1769"/>
            <end position="1967"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="160"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1575"/>
            <end position="1629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="29"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="64"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="76"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="93"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="166"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1579"/>
            <end position="1588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1613"/>
            <end position="1629"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR30334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1967" mass="222483" checksum="183EFAF8D0670229" modified="2009-07-28" version="1">MKSLRWSGRGKKSSSSSSSDDRHSEHSSQGQASPAQHWNKPEGSSHSTNSQALQKKPTVFGKHMTSSPSIYLSETSNRSNRSLSSSRASITGEDLKHEDTHTNSTVADDRTTMVTSFSAETNGTSRQQVPLPSLNNDNYDNTTFKVGWVNKAAPFQAANNRDSRVYGNSSASLDPQSGSKRGSNFRLHRAALRGCVLSLYKSGIGNVKYFDPNLPPPAHLMEQQYHETFSPAQPAQKAEEPLAVVEKQQNDLSYLSEEYPHPELKLDKDGRLLSGSNESICHMILFCPYNNDENTRKIIDVLLILPLIDKFGVFLNIFQLFGVTFTRHRVKLVNSARQHHQISAKADTLMTERLALVVKAILDVFSGFLLDDKLFSQIVDLVDIISLHDDEISTNLKVSIVERQNRLSTLCSFTKQRSSPEEIGMLVDVEQFMKLDLDDVARSVHQINHKFIREWSPQIDYSLLYESKFNEKHIYMNPMVFRNSENVHFLGRLLTTHLFVSNGAFTPHQRATVLSTWVQLGCKFEKLGDMVSWLAIATIICSVPVLRLASTWQFVPEQTLRVIFKDWVPTIVQLDRRQISSKSTNSVFILAPPNLNDSRIRESVIPFFGDLAINANDLPPDTKFKYLEKKIHRTKNAFYKWHQRIEQAKKFDNETERSALAIPVDDGKITNTIFRYWKYHLDQEQMNIDAIMDLSLALEPPKICQKQYSATSTRRSALLTGSYLPMLFNDMFPNYSIFPRDSLIGAAGVSGNETKRRPVSTQKVGAPQKLIQDYDQGSITGVERIDEPVAKEISSKVSNKQKLLKLIRDAFNIDMDMFHISDDIIFKAADDVDGRSRPASVVIETPKRMSQHSSITAARDSQDINRLSSAMESLDFFSNIGKSTGSINESFIQVVLKCATLDKLFDVLVLTTSVFSKVVDTEDLEKYFYHERKRQNGRAQSSDSASVGPLDYAFVRLLMDNDIFTETFFNTYRSFTTTTIVLENLAKRFIGAVSGAVSISRVVNSGKQRYSGSVNSSRFSGFVDATSSNSRFPAWDIKVTEEDEVNLTYMAKIQIGAMEALLHLVNLHYSDFTDCLVNNTTFLDILKIMDQEVNEEWKKRVELFSKSKLEQPEFREEVAELEQLQTTLKLLFKKLKSCYQKQLYRPLGVSKTQRLTQSYLKDFTTIPLRDLSIHMNDPVLEDKFVASFNRLSFDDYSELPKWVYGLNELVCDKLALVSNQDWVDVSQILELFSNESLVSLYQYPLHTISHSIIQSAGSQLDDLEILNVFTWLTTLSVGENEMLFDKLAPSVQMIIKLHISITQFFILQIAHPDHTIETRANICTIILQLLNFTRWKNSSMDLFHDGKQDKCTTAISPHVPSFLETCLTNAIVAPESRYYEMLWKNAHKKLAGPNAAEVKDVSNILDGIDAQIRFFVEYDESQISKPKSLSPCPGWLITRLLEISQFVPNMSIENSRMINFDKRRFVNNIIINFREMVPYVGGHSEDGSSTNFDSDLKNSILFHEIVDIGKAFRKLAKEKAAEEARKSKYQASGLFNSIIVAEVDKIKRDQKKKMELTIQDNDSKRKNMLEHAVKHKNRLSSSPSLLSLGQSPARGPPVDPEFPGAGKRSSLATTGTRSSIISNSSTHSNGSMGKKLGGFFKRPFSIGGFSSSSSTHGLNAILLSGVQPNGSISPFELPDITSSVFKDTKPVYSIRTFEIKSCIPVSGINKLPQTDHSFKIVMENGVEHIVQGTSYIDMMEWIRLINISRRYSFHSQKYKGKTSNKIFGVPIEDVCERECSIIPNIVVKLLEEIELRGLDEMGLYRVPGSVGSINALKNAFDEEGAVSNSFTLEDDRWFEINTIAGCFKLYLRELPDSLFTKEKLPLFVSLALSYKSNEIDYEQFQAGMKDALKSLPLYNFHMMKRTFEHLHRVDEHVENNRMDATNLAIVFSMSFIDQDDLASSMGPTLGALQTILQSFIRNPADFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2U3</accession>
    <name>C5E2U3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA damage-inducible protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0H07744g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DDI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556216.1">
        <property type="nucleotide sequence ID" value="XM_002556170.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2U3"/>
    <dbReference type="FunCoup" id="C5E2U3">
        <property type="interactions" value="294"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2U3"/>
    <dbReference type="MEROPS" id="A28.001"/>
    <dbReference type="GeneID" id="8294534"/>
    <dbReference type="KEGG" id="lth:KLTH0H07744g"/>
    <dbReference type="eggNOG" id="KOG0012">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020435_2_0_1"/>
    <dbReference type="InParanoid" id="C5E2U3"/>
    <dbReference type="OMA" id="LYTADPF"/>
    <dbReference type="OrthoDB" id="1047367at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05479">
        <property type="entry name" value="RP_DDI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14309">
        <property type="entry name" value="UBA_scDdi1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000072">
        <property type="entry name" value="DNA damage-inducible protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019103">
        <property type="entry name" value="Peptidase_aspartic_DDI1-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15397:SF3">
        <property type="entry name" value="DNA DAMAGE INDUCIBLE 1 HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15397">
        <property type="entry name" value="SODIUM-GLUCOSE COTRANSPORTER REGULATORY PROTEIN -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09668">
        <property type="entry name" value="Asp_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBA" evidence="9">
        <location>
            <begin position="363"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="333"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR30354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="402" mass="44399" checksum="0A407CB3C55A2BAD" modified="2009-07-28" version="1">MRLTINSEITDEIFGPLDVTAEMSLQDLIALLEFECGFDATKHNLLHKATALNSSESKTLQELQFENDELLVIKAKINNVHENSQSGASMEDMLVEQARQQILQNPALRAQIVSSNPGFESIINDATAFRQQAGPHLLQSMQGGSRQNPFGIAQTEYDELMRNPDDPANQKRIGELISQQEIDEQMRNALEFTPEAFTTVHMLYVNLEINGYPVKAFVDSGAQSTIISTKLAEKAGLSRLIDKRFSGEAHGVGVQKIIGKIHIAQVKIETQHVPCSFTVLDTHVDMLLGLDMLKRHQACIDLKDNVLRIAGVQTRFLSEAEIPESLEAKISESTSQGLNIPAPSAQLEPPKKAQTSQPHPERAFPESVVQQLMDLGFSRSEVLRSLEQANGNPDVAAALLFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3G1</accession>
    <name>C5E3G1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Tyrosine-protein phosphatase CDC14</fullName>
            <ecNumber evidence="4">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">KLTH0H13156g</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein-tyrosine phosphatase family. Non-receptor class CDC14 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="4"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556434.1">
        <property type="nucleotide sequence ID" value="XM_002556388.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3G1"/>
    <dbReference type="FunCoup" id="C5E3G1">
        <property type="interactions" value="1024"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3G1"/>
    <dbReference type="GeneID" id="8294778"/>
    <dbReference type="KEGG" id="lth:KLTH0H13156g"/>
    <dbReference type="eggNOG" id="KOG1720">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017787_1_2_1"/>
    <dbReference type="InParanoid" id="C5E3G1"/>
    <dbReference type="OMA" id="ACMLCCY"/>
    <dbReference type="OrthoDB" id="5632at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005816">
        <property type="term" value="C:spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033554">
        <property type="term" value="P:cellular response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032954">
        <property type="term" value="P:regulation of cytokinetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007096">
        <property type="term" value="P:regulation of exit from mitosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14499">
        <property type="entry name" value="CDC14_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17657">
        <property type="entry name" value="CDC14_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000038">
        <property type="entry name" value="Tyrosine-protein phosphatase CDC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000073">
        <property type="entry name" value="Tyrosine-protein phosphatase CDC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044506">
        <property type="entry name" value="CDC14_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029260">
        <property type="entry name" value="DSPn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050561">
        <property type="entry name" value="PTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23339">
        <property type="entry name" value="TYROSINE SPECIFIC PROTEIN PHOSPHATASE AND DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14671">
        <property type="entry name" value="DSPn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0469">Meiosis</keyword>
    <keyword evidence="8" id="KW-0498">Mitosis</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="16">
        <location>
            <begin position="177"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="17">
        <location>
            <begin position="262"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="385"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="404"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="422"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="448"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="481"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00069949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAR30572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="505" mass="56887" checksum="0B417B69ED595B86" modified="2009-07-28" version="1">MRKRVLLDNTIEFLRGRVYLGAYDDTPQDTDDIVFFTVEDILFYNRFHLDFGPLHIGHLYRFAVIFHEILNDPDNSGKAVVFYSSTSTRQRANSACMLCCYMLLVQGWTPHQVLQPLAQVDPPFMPFRDAGYSNADFEITIQDVVYGVWRAKERGLVNLNTFDLETYERYERVKNGDFNVLTPDFIALASPEEDAHHRTTASKSHLNGPFRSVLKFFSENNVQLVVRLNSHLYNKKHFEDLGMKHVDLIFEDGSCPDMSIVHGFVGAAETIINEGGKIAVHCKAGLGRTGCLIGAHLIYTHGFTANECIGFLRFIRPGMVVGPQQHWLYLNQNTFREWKYTMRLSLEPSEVIGDLFPLVTLEEYRRQKRSKRSLKKLVEQNNSDYTVDDHTITPPGSAHKKAFQSITGVPQNSPGQPRKGHNGSNRIENISGSKEKPADNNSDDESMQIDSSQGSATKRNGQPSNEDSAVLRQLLPKNKRAVSSGTGRRSASGAGIRKVSGTVKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3H0</accession>
    <name>C5E3H0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0H13508p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0H13508g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556443.1">
        <property type="nucleotide sequence ID" value="XM_002556397.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3H0"/>
    <dbReference type="FunCoup" id="C5E3H0">
        <property type="interactions" value="370"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3H0"/>
    <dbReference type="GeneID" id="8294804"/>
    <dbReference type="KEGG" id="lth:KLTH0H13508g"/>
    <dbReference type="eggNOG" id="KOG2061">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031771_0_0_1"/>
    <dbReference type="InParanoid" id="C5E3H0"/>
    <dbReference type="OMA" id="LMPKMIL"/>
    <dbReference type="OrthoDB" id="443682at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007320">
        <property type="entry name" value="PDCD2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47524">
        <property type="entry name" value="20S RRNA ACCUMULATION PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47524:SF1">
        <property type="entry name" value="20S RRNA ACCUMULATION PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04194">
        <property type="entry name" value="PDCD2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Programmed cell death protein 2 C-terminal" evidence="2">
        <location>
            <begin position="291"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="162"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR30581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="404" mass="45591" checksum="5A240E03DDB5426D" modified="2009-07-28" version="1">MSRIEELESDSEENFGSKSSQVFLGFVDAAIKESDQVTIEDSFIGGEPVWLHPDSIPGKELLECGACKSRNNMKLILQAFAPLDPEQVEDATRKAGLQSNASQNINPSDERVLYVFICTKCSRKPHSVRCIRGVRKSAVDDVLGAKMEDHGTEKDFNINPFDLNQNTSNPFSSSSNETADTNPFTKSASTPFESTQKKANVPAEESNNPKNARKQHDALPDKKFEGKFPGFFLYVDEESFKNKTPDHLKLPKNLKIDKTAFELSEEDDESIGKSPIKLDPRTEKLSKFLDDDVFQKFQEIVGYNPLQVLRYDFGGRPLYYSATNVELEKIVPSPGWNPSSKRVFELQLMPKMIMDLEVTVSVTEGMDWGTILIFTDVENYIPKFDEHGVGYVEECVRVQWESDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3Q1</accession>
    <name>C5E3Q1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0H15422p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0H15422g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556524.1">
        <property type="nucleotide sequence ID" value="XM_002556478.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3Q1"/>
    <dbReference type="FunCoup" id="C5E3Q1">
        <property type="interactions" value="79"/>
    </dbReference>
    <dbReference type="GeneID" id="8294890"/>
    <dbReference type="KEGG" id="lth:KLTH0H15422g"/>
    <dbReference type="eggNOG" id="ENOG502SABW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_816542_0_0_1"/>
    <dbReference type="InParanoid" id="C5E3Q1"/>
    <dbReference type="OMA" id="NTHVKEH"/>
    <dbReference type="OrthoDB" id="3981230at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="316"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR30662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="340" mass="37071" checksum="DB3852994D58C425" modified="2009-07-28" version="1">MQALTRTPTKQINNNDAAFGLAHHTRTARLTPRAQVVEEALAAPPLRLGSKSGYRFPARSVESPSKPSRAHRRRPVSIAFADGSYQMPVLTKDVPELNSDEDTLSDDSCASGNSIISPSTSLSVTPPEACAFENQDSNSPPSLKDFLLSSALMHSSLLNDGALHDGPEGHSEANTTDILPLFRTHSRKQMPIVPKPLPHRHRRERVLKSILKKPSSPDFLNFVVTATNGSLVDATIFATEINSCSNKVPLPTTVWEKVTIPVNVEIRDKFKKSRAQLIGGYYDDCGTTEDDQPQEGPSDIKDAAVIRGYECSFTGTKADDGNEKGLSMETGKSLRWAHKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3Q8</accession>
    <name>C5E3Q8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0H15576p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0H15576g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIP5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556531.1">
        <property type="nucleotide sequence ID" value="XM_002556485.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3Q8"/>
    <dbReference type="FunCoup" id="C5E3Q8">
        <property type="interactions" value="66"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3Q8"/>
    <dbReference type="GeneID" id="8294897"/>
    <dbReference type="KEGG" id="lth:KLTH0H15576g"/>
    <dbReference type="eggNOG" id="KOG2789">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009068_2_0_1"/>
    <dbReference type="InParanoid" id="C5E3Q8"/>
    <dbReference type="OMA" id="ISEPANC"/>
    <dbReference type="OrthoDB" id="21471at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd24139">
        <property type="entry name" value="SIP5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039301">
        <property type="entry name" value="Sip5/DA2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31315">
        <property type="entry name" value="PROTEIN SIP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31315:SF1">
        <property type="entry name" value="PROTEIN SIP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="291"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="349"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="418"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="18"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="44"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="294"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="371"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="424"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR30669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="486" mass="54714" checksum="A2522019517EC571" modified="2009-07-28" version="1">MGNVPGKLENEGVARPNRSMSASAAQTPSGRNGTTTAGARSQRTSSLVGTLLSSRRRSSSSEVNGNGNPYKRKSTKDKEKMEERHVKQLIVKYNETVDGGFLAPFGSCGTDKLDYDAKVVKNLIMERRLAPFYTPLQDFDDSWSREEVIKIIEALPLHAPFNENLETFEDVPIGDLSQNDIDHLIDKTLSRREQRRMRSKIFKARLYKKRIIWQERENERFFESKIEGKQSKLQSNPNLPSDDLKYAMYSSGAECPICFLYYPQPINMSRCCLQPICSECFVQIKRAEPHFPHDEEDPSNPQDEEKDPNMLTSEPTNCPYCATPDFGITYEAPADRKVGLQGLAPSSYVYHKKDSPEQDSVPRTPDRRGSLASTDQRVVTSDSLRPDWQVKLNKERMRLARRSANATAIHVSNQLVTPGHQASAGVTSTSAPRSNSNDNAVEFQHSSPTPLWPANATAAEIENHMIEQAIKLSLAEHERENSPKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3R6</accession>
    <name>C5E3R6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Eukaryotic translation initiation factor 3 subunit I</fullName>
            <shortName evidence="7">eIF3i</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Eukaryotic translation initiation factor 3 39 kDa subunit homolog</fullName>
            <shortName evidence="7">eIF-3 39 kDa subunit homolog</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">TIF34</name>
        <name evidence="9" type="ordered locus">KLTH0H15752g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the WD repeat STRAP family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the eIF-3 subunit I family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556539.1">
        <property type="nucleotide sequence ID" value="XM_002556493.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3R6"/>
    <dbReference type="FunCoup" id="C5E3R6">
        <property type="interactions" value="1183"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3R6"/>
    <dbReference type="GeneID" id="8294905"/>
    <dbReference type="KEGG" id="lth:KLTH0H15752g"/>
    <dbReference type="eggNOG" id="KOG0643">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043845_0_1_1"/>
    <dbReference type="InParanoid" id="C5E3R6"/>
    <dbReference type="OMA" id="VWFSHNG"/>
    <dbReference type="OrthoDB" id="24966at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071541">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex, eIF3m"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000127">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 subunit I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03008">
        <property type="entry name" value="eIF3i"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027525">
        <property type="entry name" value="eIF3i"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19877">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19877:SF1">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24805">
        <property type="entry name" value="EIF3I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 7" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 8" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="6"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="48"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="191"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR30677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="350" mass="38784" checksum="CEA02B7796E95283" modified="2009-07-28" version="1">MRPILLKGHERSLTQVKFNREGDLVFTCAKDNVASVWYSINGERLGTFEGHQGTIWSIDVDQSTQYAVTGSADFSIKLWKVEDGTNVFTWKTKTPVRRVEFSPAGDRILAVLDGVMGYAGSVSVYELKRDAQTQEVVDVVEQPLFDILTREGFEIAAVAAWSFGGNYIVAGHKDGKVSKYDGHTGDFVDAAELHTQSVSDIQFSPDGTYFITSSRDSAARLVDMETLEVLKTYETDCPLNSACITPLKQFVILGGGQDAKDVTTTSSREGKFEARFYHKIFQDEIGRVKGHFGPLNYVAVSPQGTSYASGGEDGFVRLHHFDKSYFDFKYDVEKSAEAQKHMQELESAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3S8</accession>
    <name>C5E3S8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cysteine protease RIM13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0H16016g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C2 family. PalB/RIM13 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556551.1">
        <property type="nucleotide sequence ID" value="XM_002556505.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3S8"/>
    <dbReference type="FunCoup" id="C5E3S8">
        <property type="interactions" value="34"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3S8"/>
    <dbReference type="GeneID" id="8294917"/>
    <dbReference type="KEGG" id="lth:KLTH0H16016g"/>
    <dbReference type="eggNOG" id="KOG0045">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_395483_0_0_1"/>
    <dbReference type="InParanoid" id="C5E3S8"/>
    <dbReference type="OMA" id="GWLPQII"/>
    <dbReference type="OrthoDB" id="167576at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036213">
        <property type="entry name" value="Calpain_III_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051297">
        <property type="entry name" value="PalB/RIM13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46143">
        <property type="entry name" value="CALPAIN-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46143:SF1">
        <property type="entry name" value="CALPAIN-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49758">
        <property type="entry name" value="Calpain large subunit, middle domain (domain III)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="84"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="97"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00042255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR30689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="707" mass="80585" checksum="B8E9DA3345CC8E8A" modified="2009-07-28" version="1">MSLKNAELAWKTLNDAIWNAQVLGKVDKTRWEEVRKTVRELGDDEISRAFTRHEECRGKFGFETRFLWLTSVLNGRRYPPVNADNGGPVSWNEETNETSSNRYASDQHAPITTCTDRTQPPPKQFRNPEIEQNSRISDCSFVASLINIRCQCPEYVKVSRVGSSSVFNVNLHFNGASNRLVRVKLSNIPSDSEGGRLAILSENAEDAALELAYFQIRNGSKHTFAGSNAAIDTFLLGGFVPEIYKIKEDTCNYIRNLLQKGICLVTLGTGQTVKSSQYLINHDYPVLGLDREGRIIVRDPLCISKTFPLNEQAFLENFNVAYVNWKCSLLFSEHQKVHFKYSAQKCNASMSLLNKPIFRLKNESKSSEPLWVLLERHLGNYNVKNNGCAVREATRDLYPLDTEKGSNTGFHLLKLTLEPGDFKYIYCHSDVSANFTLHLLHVSRCVRAERFNANSISASVEDEWNTATDHGPFSNPTYYMNPTFELIIDAKDQSTHYANIQLESIGNSLVNVQIFHHDDHDLTRPILFDGHYENKVHVRRAVPLSTGTPYKIICSAYEKYTNKSFRLSVTDPAEQCRLLLRRIYPQFGSRMYHTKHEFFWPQNSNRKKIEIVPSATMELQIRLLPLQFSSALSIRCNIFLSDTGQAILTNNDFSPTPRHGKVIEKFKVIKSQRLTLIIEKDGPSTISSDVPIRLEIGSDFEILLYDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBA2</accession>
    <name>C5DBA2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0A00880p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0A00880g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
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    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_002551455.1"/>
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    <dbReference type="GeneID" id="8290292"/>
    <dbReference type="KEGG" id="lth:KLTH0A00880g"/>
    <dbReference type="eggNOG" id="KOG0272">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="HOGENOM" id="CLU_000288_57_20_1"/>
    <dbReference type="InParanoid" id="C5DBA2"/>
    <dbReference type="OMA" id="LNEPICY"/>
    <dbReference type="OrthoDB" id="540662at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
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        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:U4 snRNA binding"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:U6 snRNA binding"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:mRNA splicing, via spliceosome"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="U4/U6 small nuclear ribonucleoprotein Prp4"/>
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        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
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        <property type="entry name" value="WD40_rpt"/>
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        <property type="entry name" value="PRE-MRNA PROCESSING FACTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
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        <property type="entry name" value="SFM"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
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        <property type="entry name" value="PRP4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Pre-mRNA processing factor 4 (PRP4)-like" evidence="5">
        <location>
            <begin position="47"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="224"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="264"/>
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        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="306"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="348"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="433"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="93"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="97"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR21059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="471" mass="52376" checksum="C3529FA573521049" modified="2009-07-28" version="1">MNGQGTISFDEIRVDTSYIVEDGGKKADDTLKSLEFDREQKLTIVPTDDTEVRESLVHLGQPEALENESNRDRRDRLSDLLYQNPDIRAQFLERQAKSPSLETGPSEASESEDEEFYTPASGSLVECRQFLIQDSLGRARERLRNERGHADSQDVSTTIVQRRKLNAELSKYELWGSQVASSRPVSGVLLSPNQKSVACGSWHGDVKIIDSETLEILASFDAIHDDKIGGLDWSSDSTLLVTGGADRIVKVWKPSSPNDSTAVLKGHEGRVVKVKFHPSDRYVASASFDMTWRLWDVERQVELQLQEGHAKEVYCLDFQCDGSLLCSAGLDSIGRIWDMRSGQSLMILEGHAKPIYGVSWSPNGHHVATGSADGTVKVWDIRKVGNPFSILAHNNIVSDVKFDKVSGRTLVSSSYDKTVSVFASDSWLRLASLKGHTDKVMSIDISHDFSHLYSSGWDRSVKLWKLGVCNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBD8</accession>
    <name>C5DBD8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Obg-like ATPase 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">OLA1</name>
        <name evidence="7" type="ordered locus">KLTH0A01782g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 4">Hydrolyzes ATP, and can also hydrolyze GTP with lower efficiency. Has lower affinity for GTP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family. YchF/OLA1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002551491.1"/>
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    </dbReference>
    <dbReference type="STRING" id="559295.C5DBD8"/>
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    <dbReference type="KEGG" id="lth:KLTH0A01782g"/>
    <dbReference type="eggNOG" id="KOG1491">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018395_1_2_1"/>
    <dbReference type="InParanoid" id="C5DBD8"/>
    <dbReference type="OMA" id="VLRCFDN"/>
    <dbReference type="OrthoDB" id="424823at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04867">
        <property type="entry name" value="TGS_YchF_OLA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01900">
        <property type="entry name" value="YchF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.300:FF:000001">
        <property type="entry name" value="Ribosome-binding ATPase YchF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000001">
        <property type="entry name" value="Ribosome-binding ATPase YchF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.300">
        <property type="entry name" value="TGS-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00944">
        <property type="entry name" value="YchF_OLA1_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004396">
        <property type="entry name" value="ATPase_YchF/OLA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023192">
        <property type="entry name" value="TGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013029">
        <property type="entry name" value="YchF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041706">
        <property type="entry name" value="YchF_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00092">
        <property type="entry name" value="redox-regulated ATPase YchF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305">
        <property type="entry name" value="OBG GTPASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305:SF11">
        <property type="entry name" value="OBG-LIKE ATPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06071">
        <property type="entry name" value="YchF-GTPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006641">
        <property type="entry name" value="CHP00092"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="TGS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="5">
        <location>
            <begin position="21"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="TGS" evidence="6">
        <location>
            <begin position="305"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="30"/>
            <end position="35"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00059898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR21095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="393" mass="44077" checksum="CA45DBFE47CB13B7" modified="2009-07-28" version="1">MPPKKQVEEKKVLLGRPGNNLKAGIVGLANVGKSTFFQAITRCPLGNPANYPFATIDPEEARVIVPSPRFDALCDIYKPASKVPAHLTVYDIAGLTKGASAGEGLGNAFLSHIRAVDSIYQVVRCFDDAEIIHIEGDVDPVRDLDIISTELRLKDIEFAEKHLENVHKITKRGGQSLEVKQKKEEAILVEKIIELLKSGQRVANQNWTTKEVEVINSMFLLTAKPSIYLINLSERDFIRKKNKHLMRIKEWIDKYSTGDLVIPFSVCLEERLSHMSPEEAEEELKNLKVESALPKIVTTMRQKLDLISFFTSGADEVREWTIRRGTKAPQAAGVIHNDLMNTFILAQVMKYEDVVEYKDEVAIKAAGKMQQKGKEYVVEDGDVIYFRAGAGKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBI1</accession>
    <name>C5DBI1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0A02816p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0A02816g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551580.1">
        <property type="nucleotide sequence ID" value="XM_002551534.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBI1"/>
    <dbReference type="FunCoup" id="C5DBI1">
        <property type="interactions" value="245"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBI1"/>
    <dbReference type="GeneID" id="8290379"/>
    <dbReference type="KEGG" id="lth:KLTH0A02816g"/>
    <dbReference type="eggNOG" id="ENOG502QVDM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_409922_0_0_1"/>
    <dbReference type="InParanoid" id="C5DBI1"/>
    <dbReference type="OMA" id="VYDPIHD"/>
    <dbReference type="OrthoDB" id="5413003at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038014">
        <property type="entry name" value="Ies1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37287">
        <property type="entry name" value="INO EIGHTY SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37287:SF1">
        <property type="entry name" value="INO EIGHTY SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="377"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="406"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="438"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="467"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="645" mass="73987" checksum="87C46BF3E532FD5F" modified="2009-07-28" version="1">MGNRVYDPIHDVFQNKSEQEDVPPNEKLAGAEAPYPEEEDGDSTQSDDNATPMNESGPHKRRTKEPSKYMRHLKKPDGEFFTRKDIQFEFLHELCSDRRALFTNRYQDFFTIAPTEESEPLNVTDESYVARKFIKNEKLTFSEYYLLTIASSTKCSKILRDKLLFDRNVAFSTCALSLLVNTGRLNTTINFFLEMTSQLRTFHSIPCLQRDARDPKSLQDTPRLKSILKNLPLGNENLPLTEYYEAPVAGKRLDSNPVNLMFHLCDNVALVNLKFIQEYISGASDLSFFDILENPDYDPSQRAQILLWLVYVHLETDMTDSAIEKSLELFEVDGKFKLESAPENVDIDTPEEVEFGESQKTKRREFLVKNNRLAHDGYRRSDANRPLMEQTRLDNSSAEDVESIDEDKSFTEKTPSRAVQRANQRASRRTGQKPGPKPGAKAAARALAPKRQSRKHAREQANDSMEEEHMPETTSKLEDEDFLAEEEPKLQVQLQSQSQPDDPEALQAPAKKKQKRKTGPAAPASQVQRNEALDEKKKDEMEALIEFDKSEEVAQHRTQDALMQELERAQALTRRKREELGLVKMFYEFEDVTMATVIGVRGKKRKKFKDGLLGFETDFIRTISGAKRAMLTAQHDANEDIYEFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBL4</accession>
    <name>C5DBL4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0A03564p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0A03564g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551613.1">
        <property type="nucleotide sequence ID" value="XM_002551567.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBL4"/>
    <dbReference type="FunCoup" id="C5DBL4">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="GeneID" id="8290413"/>
    <dbReference type="KEGG" id="lth:KLTH0A03564g"/>
    <dbReference type="HOGENOM" id="CLU_709936_0_0_1"/>
    <dbReference type="InParanoid" id="C5DBL4"/>
    <dbReference type="OMA" id="YRKFIQC"/>
    <dbReference type="OrthoDB" id="4055114at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR21171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="389" mass="44479" checksum="4B6763B6A4214C13" modified="2009-07-28" version="1">MVFSNPICQTDDNPGSLDLALALSLIKQRNPRLWHKLASQGFFQETAGASELFPAERHVVKGSAANKADTGIESIGESFLYKFTTNNISSHIERATAFLGCNRHTWKCLSSQISTLESFGCGLEESDIAKWVLPNYRLLKWLYAKDPPQLQKRDRALRTVNSFQLLLISHYAFSGERNKVVHVLCQSLIYRFIKDELISFGPSKVRVLKLLRDYTDSNVVQVADVSLFSLSEGHLKLVKLILNIRISFQDHEVLSKTLQFLLKLLESLVVCLIKLNSSRYSPARKPRAYEIMYKARSKLMNYQYVTKVLEACHLLSRKEATIRLKISFTFVLKLVTSVEWLNAFDNSGAGQNELMRTLREHRTYIISSLLVLNDSRLISKFLKTRWPAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBP5</accession>
    <name>C5DBP5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Polyprenal reductase</fullName>
            <ecNumber evidence="5">1.3.1.94</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0A04312g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Plays a key role in early steps of protein N-linked glycosylation by being involved in the conversion of polyprenol into dolichol. Acts as a polyprenal reductase that mediates the reduction of polyprenal into dolichal in a NADP-dependent mechanism. Dolichols are required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-glycosylation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 5">
            <text>a di-trans,poly-cis-dolichal + NADP(+) = a di-trans,poly-cis-polyprenal + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:80727"/>
            <dbReference type="Rhea" id="RHEA-COMP:19536"/>
            <dbReference type="Rhea" id="RHEA-COMP:19537"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:231623"/>
            <dbReference type="ChEBI" id="CHEBI:231637"/>
            <dbReference type="EC" id="1.3.1.94"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="9 5">
            <dbReference type="Rhea" id="RHEA:80729"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the steroid 5-alpha reductase family. Polyprenal reductase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.94" evidence="5"/>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551644.1">
        <property type="nucleotide sequence ID" value="XM_002551598.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBP5"/>
    <dbReference type="FunCoup" id="C5DBP5">
        <property type="interactions" value="372"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBP5"/>
    <dbReference type="GeneID" id="8290447"/>
    <dbReference type="KEGG" id="lth:KLTH0A04312g"/>
    <dbReference type="eggNOG" id="KOG1640">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044409_0_0_1"/>
    <dbReference type="InParanoid" id="C5DBP5"/>
    <dbReference type="OMA" id="RFYETNF"/>
    <dbReference type="OrthoDB" id="541710at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003865">
        <property type="term" value="F:3-oxo-5-alpha-steroid 4-dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160198">
        <property type="term" value="F:polyprenal reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102389">
        <property type="term" value="F:polyprenol reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016095">
        <property type="term" value="P:polyprenol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001104">
        <property type="entry name" value="3-oxo-5_a-steroid_4-DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039698">
        <property type="entry name" value="Dfg10/SRD5A3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14624">
        <property type="entry name" value="DFG10 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14624:SF0">
        <property type="entry name" value="POLYPRENOL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02544">
        <property type="entry name" value="Steroid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50244">
        <property type="entry name" value="S5A_REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="67"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="192"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="3-oxo-5-alpha-steroid 4-dehydrogenase C-terminal" evidence="6">
        <location>
            <begin position="143"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU367081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR21202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="247" mass="28151" checksum="557CD520649FB736" modified="2009-07-28" version="1">MDLNPRSLFELAFYSSFLLAITSVLLAKWRLPTLLKYGKTLQGVPSKGGILGSLQSLTVPKKWFGHFYVYSTALALLNVCFLRGFASLLVLTHSARRLYETRCVSKFGKDSRIHLSHYLVGLWFYTAVNCAVFVDRTRTRSPLARLVAVIVFVLSSLDQYRNHLHLSKLVKYTLPTYGLFQLVSSPHYFDEILIYLSLAIYTSSLKMFLCLVWVIVNLSTSALETRSWYAKKFPRAAPSFAIIPYLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBP6</accession>
    <name>C5DBP6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">3-oxoacyl-[acyl-carrier-protein] synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0A04334g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a fatty acyl-[ACP] + malonyl-[ACP] + H(+) = a 3-oxoacyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:22836"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9916"/>
            <dbReference type="Rhea" id="RHEA-COMP:14125"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78776"/>
            <dbReference type="ChEBI" id="CHEBI:138651"/>
            <dbReference type="EC" id="2.3.1.41"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 9 11">Belongs to the thiolase-like superfamily. Beta-ketoacyl-ACP synthases family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551645.1">
        <property type="nucleotide sequence ID" value="XM_002551599.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBP6"/>
    <dbReference type="FunCoup" id="C5DBP6">
        <property type="interactions" value="477"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBP6"/>
    <dbReference type="GeneID" id="8290448"/>
    <dbReference type="KEGG" id="lth:KLTH0A04334g"/>
    <dbReference type="eggNOG" id="KOG1394">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_69_2_1"/>
    <dbReference type="InParanoid" id="C5DBP6"/>
    <dbReference type="OMA" id="QIGHCLG"/>
    <dbReference type="OrthoDB" id="5334845at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00834">
        <property type="entry name" value="KAS_I_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000018">
        <property type="entry name" value="3-oxoacyl-[acyl-carrier-protein] synthase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017568">
        <property type="entry name" value="3-oxoacyl-ACP_synth-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000794">
        <property type="entry name" value="Beta-ketoacyl_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018201">
        <property type="entry name" value="Ketoacyl_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005589">
        <property type="entry name" value="PRK07314.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11712:SF336">
        <property type="entry name" value="3-OXOACYL-[ACYL-CARRIER-PROTEIN] SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11712">
        <property type="entry name" value="POLYKETIDE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000447">
        <property type="entry name" value="KAS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00825">
        <property type="entry name" value="PKS_KS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00606">
        <property type="entry name" value="KS3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52004">
        <property type="entry name" value="KS3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6 9" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 9" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ketosynthase family 3 (KS3)" evidence="12">
        <location>
            <begin position="2"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="active site" description="For beta-ketoacyl synthase activity" evidence="10">
        <location>
            <position position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR000447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000447-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS52004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR21203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="424" mass="44988" checksum="2E7230C997003F81" modified="2009-07-28" version="1">MSTRVVVTGLGALTPLGKTVAESWAALLAGKSGIRPIGELANAESYERHCPPSLAVADVKEFDPHAYGNLFTSQDLRRMSQFTQFAVVAAQEALRDANLIETLQKGRNDRVGCVIGSGIASVHDMYDSIVEFDRGKRLSPSFVPKILSNMACGNVSIKFQLRGVSHCVSTACATGNNAIGDAYNFIRLGYNDVCLAGASEASVHPLALAGFLRAKSITPSGISRPFDKERNGFVLGEGAGLLVLESLKHAQDRGARIYAEVEGYGLTSDAHHITSPSENGDGARRAMEMALNNQTRSQVDYVNAHATSTVLGDRAEAQAISNILSGDGKDAIKVSSNKGAIGHLLGAAGAVESIFTIKSLQDGVVPHTLNLHSVGGASGDEAENFERLDLVRGAPAKHDIRRALNNSFGFGGINSSIVFRRWEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DC54</accession>
    <name>C5DC54_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0A08052p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0A08052g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551803.1">
        <property type="nucleotide sequence ID" value="XM_002551757.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DC54"/>
    <dbReference type="FunCoup" id="C5DC54">
        <property type="interactions" value="170"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DC54"/>
    <dbReference type="GeneID" id="8290617"/>
    <dbReference type="KEGG" id="lth:KLTH0A08052g"/>
    <dbReference type="eggNOG" id="KOG1575">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_2_0_1"/>
    <dbReference type="InParanoid" id="C5DC54"/>
    <dbReference type="OMA" id="MPAWRFM"/>
    <dbReference type="OrthoDB" id="48988at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19079">
        <property type="entry name" value="AKR_EcYajO-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000004">
        <property type="entry name" value="Oxidoreductase, aldo/keto reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050523">
        <property type="entry name" value="AKR_Detox_Biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43364:SF15">
        <property type="entry name" value="ARYL-ALCOHOL DEHYDROGENASE AAD16-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43364">
        <property type="entry name" value="NADH-SPECIFIC METHYLGLYOXAL REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="2">
        <location>
            <begin position="18"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR21362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="338" mass="38329" checksum="0176C64652B81945" modified="2009-07-28" version="1">MPIVQQVRLGSSGLKISPIIVGCMSFGSKSWAPWLLDDKEEIFKILKYCYDNGLRTFDTADIYSNGLSERLIGEFLRNYNINRETVVILSKVNFPVDESLDLSVKSIFNVDEVTALTLTNQRGLSRKHIIDGVAKSVERLGTYIDVLQIHRFDSETTMEETMRALNDVVESGHARYIGASSMLATEFSDLQFTVERNGWFKFISSQSQYNLLYREDERELIPYAKRHGIGLIPWSPNARGLLTRPVGVATERSSSDPRFQKLTNAGMEIINRVEEVANRRNVSMVVASNAWVLHKGCHPIVGMSSTARVDEAVQALTFKLTDEEVKYIEEPYRPKSYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCF5</accession>
    <name>C5DCF5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Mitochondrial import inner membrane translocase subunit TIM14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Presequence translocated-associated motor subunit PAM18</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0B02640g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. In the complex, it is required to stimulate activity of mtHSP70 (SSC1).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the TIM14 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551904.1">
        <property type="nucleotide sequence ID" value="XM_002551858.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCF5"/>
    <dbReference type="FunCoup" id="C5DCF5">
        <property type="interactions" value="60"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCF5"/>
    <dbReference type="GeneID" id="8290735"/>
    <dbReference type="KEGG" id="lth:KLTH0B02640g"/>
    <dbReference type="eggNOG" id="KOG0723">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017633_13_0_1"/>
    <dbReference type="InParanoid" id="C5DCF5"/>
    <dbReference type="OMA" id="SHPPIME"/>
    <dbReference type="OrthoDB" id="240298at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001405">
        <property type="term" value="C:PAM complex, Tim23 associated import motor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000001">
        <property type="entry name" value="Import inner membrane translocase subunit tim14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12763">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12763:SF28">
        <property type="entry name" value="GEO10507P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0811">Translocation</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="J" evidence="13">
        <location>
            <begin position="155"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00040828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00041716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR21466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="211" mass="23120" checksum="6156AF193EBDDB03" modified="2009-07-28" version="1">MGCDAMRCPCRLSKNFFQLNFYSHFLPELENSLETGRRKAKWTGEKDEKASNMAPIEAPQLAIPGEPSAVAPQAFNELVPATAAPVGSAKKSMDEKFDEALAFMGRHPVMSGVGGFLGLYFAAGAFKSVSRMMGGGPKAAQFAKGGFDPKMNTKEALQILNLSENNLNRKKLKEVHRRIMLANHPDKGGSPYLATKINEAKDFLEKRGLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCY7</accession>
    <name>C5DCY7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0B06886p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0B06886g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552086.1">
        <property type="nucleotide sequence ID" value="XM_002552040.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCY7"/>
    <dbReference type="FunCoup" id="C5DCY7">
        <property type="interactions" value="1001"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCY7"/>
    <dbReference type="GeneID" id="8290926"/>
    <dbReference type="KEGG" id="lth:KLTH0B06886g"/>
    <dbReference type="eggNOG" id="KOG4210">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027451_1_1_1"/>
    <dbReference type="InParanoid" id="C5DCY7"/>
    <dbReference type="OMA" id="RVWVNQL"/>
    <dbReference type="OrthoDB" id="1875751at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12397">
        <property type="entry name" value="RRM2_Nop13p_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000863">
        <property type="entry name" value="Nucleolar protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034226">
        <property type="entry name" value="Nop13/Rnp24_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF95">
        <property type="entry name" value="NUCLEOLAR PROTEIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="123"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="235"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="83"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="309"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="83"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="326"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="372"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR21648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="396" mass="44799" checksum="F7F33C3E2DC81704" modified="2009-07-28" version="1">MASNNKSEEVVDKVEDKSAKEQEIEKALQDQTKKRKAEDEIEIDLNQTVPLSKKQKRLLRRGKVTLEELNEKFNLDPSSVKEYQKEIEGKTQSEKKEGVEEGEEEVQEDASSASQKAKKDKKFGVWIGNLTFDTSKEDLCRFFIAKTKELDPDSRVEESDILRVNLPLAQNDGKQVKNKGFAHMNFRTQKQMDAVIALSESHLNGRNMLIKNSASFDGRPDKNDLISMSKNPPSRILFVGNLSFDTTDELLKKHFQHCGEIVKIRMATFQDTGKCKGFAFVDFKNEEGPTNALKDKTCRRIAGRPLRMEFGEDRSKRQVKRRVPQASETLSSTSGPSLREEPSTSAPPIRERKPVKKHREPSYKDKNNRLKSSVALASAPRASAAIVPSQGKKITF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-03-08" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DD02</accession>
    <name>SWT21_LACTC</name>
    <protein>
        <recommendedName>
            <fullName>Protein SWT21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">SWT21</name>
        <name type="ordered locus">KLTH0B07216g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in mRNA splicing. Helps to stabilize the U1 snRNP-5' splice site interaction.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Associates with snRNPs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SWT21 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552101.1">
        <property type="nucleotide sequence ID" value="XM_002552055.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DD02"/>
    <dbReference type="FunCoup" id="C5DD02">
        <property type="interactions" value="36"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DD02"/>
    <dbReference type="GeneID" id="8290941"/>
    <dbReference type="KEGG" id="lth:KLTH0B07216g"/>
    <dbReference type="eggNOG" id="ENOG502QVPI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_662333_0_0_1"/>
    <dbReference type="InParanoid" id="C5DD02"/>
    <dbReference type="OMA" id="VICQDIF"/>
    <dbReference type="OrthoDB" id="239865at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051150">
        <property type="entry name" value="SWT21/TCAB1_mRNA_Telomere"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13211">
        <property type="entry name" value="TELOMERASE CAJAL BODY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13211:SF0">
        <property type="entry name" value="TELOMERASE CAJAL BODY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0507">mRNA processing</keyword>
    <keyword id="KW-0508">mRNA splicing</keyword>
    <keyword id="KW-0539">Nucleus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000405685" description="Protein SWT21">
        <location>
            <begin position="1"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="422" mass="47406" checksum="21BAB69D1E8992C7" modified="2009-07-28" version="1">MNRVRKVYDTLDTFDGRDVLQSWKREGSRWLDQVENSTYQFPELESSNYERTKGRVPTEHIDLRSPIICTKLSWSQDGTSLVAVFNDYGVRQYLLPEEEGTGLVPFKRFFRAQPAVDCCIHPEYSLFNDSDACNVMLASSRDLPIQLLSLSPHAREHRPLFSYSVVNAENERFETMFSLSFSHYSGFLAGTSRNKVVAYDLNYKSPIWSSDGYGKPLRGTSARRAIISCFDSPSDSESVLRYAGTYRCDIYGIDLRTKELNFLANKQTLELKENSAGVAELLFSVNKHYLYVIKRNSQTIDVLDARSSFAKINELYLSFRIGSQKIIATLDLINGLLIGTHDGKILQWASNMVEFGGLLPRTPEGPAKDSAVSKVFDTSYAGSRINILASSPSNPHLIAGSFSPDKFEADHTAKSGLFVVDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>C5DD59</accession>
    <name>C5DD59_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">phosphopyruvate hydratase</fullName>
            <ecNumber evidence="3">4.2.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0B08514g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(2R)-2-phosphoglycerate = phosphoenolpyruvate + H2O</text>
            <dbReference type="Rhea" id="RHEA:10164"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="4.2.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="11">Mg(2+) is required for catalysis and for stabilizing the dimer.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the enolase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.11" evidence="3"/>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552158.1">
        <property type="nucleotide sequence ID" value="XM_002552112.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DD59"/>
    <dbReference type="SMR" id="C5DD59"/>
    <dbReference type="FunCoup" id="C5DD59">
        <property type="interactions" value="1348"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DD59"/>
    <dbReference type="GeneID" id="8290998"/>
    <dbReference type="KEGG" id="lth:KLTH0B08514g"/>
    <dbReference type="eggNOG" id="KOG2670">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031223_0_0_1"/>
    <dbReference type="InParanoid" id="C5DD59"/>
    <dbReference type="OMA" id="RCMMSHR"/>
    <dbReference type="OrthoDB" id="1739814at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00187"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000015">
        <property type="term" value="C:phosphopyruvate hydratase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004634">
        <property type="term" value="F:phosphopyruvate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03313">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.10:FF:000001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.120:FF:000002">
        <property type="entry name" value="Enolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="entry name" value="Enolase-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="entry name" value="Enolase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00318">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000941">
        <property type="entry name" value="Enolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020810">
        <property type="entry name" value="Enolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020809">
        <property type="entry name" value="Enolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020811">
        <property type="entry name" value="Enolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01060">
        <property type="entry name" value="eno"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902:SF1">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00113">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03952">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001400">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00148">
        <property type="entry name" value="ENOLASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00002">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00178">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01192">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01193">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="Enolase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="Enolase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00164">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enolase N-terminal" evidence="13">
        <location>
            <begin position="3"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Enolase C-terminal TIM barrel" evidence="12">
        <location>
            <begin position="144"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="9">
        <location>
            <position position="212"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="346"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="321"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="321"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="373"/>
            <end position="376"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="397"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR21720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="437" mass="46707" checksum="60FBD8F3A7802ACB" modified="2009-07-28" version="1">MAISKVHARQVYDSRGNPTVEVELTTEKGTFRSIVPSGASTGAYEAIELRDGDKSKWLGKGVLKAVDNVNSVIAPALVKANVDVKNQKAVDELLLSLDGTPNKSKLGANAVLGVSMAAAKAAAAEKNVPLYKHLADLSNSKQDSYVLPVPFLNVLNGGSHAGGALALQEFMIAPTGAESFSEAMRMGSEVYHNLKSLTKKRYGPSAGNVGDEGGVAPNIQTAEEALDLIVDAIKAAGYEGKISIGLDSASSEFYKDGLYDLDFKNPDSDKSKWLTGKQMAEMYASLVSKYPIVSIEDPFAEDDWEAWSHYYKTAGIQIVADDLTVTNPLRIKTAIEKKAADALLLKVNQIGSLSESIEAAQDSFAAGWGVMVSHRSGETEDTFIADLVVGLRTGQIKTGAPARSERLAKLNQLLRIEEELGSKAVFAGKKFHNGQNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDI7</accession>
    <name>C5DDI7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0C01298p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0C01298g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552286.1">
        <property type="nucleotide sequence ID" value="XM_002552240.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDI7"/>
    <dbReference type="FunCoup" id="C5DDI7">
        <property type="interactions" value="298"/>
    </dbReference>
    <dbReference type="GeneID" id="8291144"/>
    <dbReference type="KEGG" id="lth:KLTH0C01298g"/>
    <dbReference type="eggNOG" id="ENOG502QQPQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_327620_0_0_1"/>
    <dbReference type="InParanoid" id="C5DDI7"/>
    <dbReference type="OMA" id="VRMDPIN"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR21848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="884" mass="103039" checksum="B04A160DA5995872" modified="2009-07-28" version="1">MISCRDICVRARIGSQLRFYRKRLKPRYNHVPQVSSLQSELLQPRELRLQAHSPESVERDLRRLGFREIDESGPVDDMLVEYLESSKSPFKKSSKHLNSLKSTLVNNKSPNQDRMKALFDFLLKESEQEVRRLESMGPEQLQMLQTIHDSEKSEHSAKEISSERELEKAILRELMESSQDEEGRSLLPNTECLFRVLSDLNMNRLAKADIISVEQMVGAFEISKLLPAYKLRQRGILLSGHLIYSLGNVRMDPINESFYIESLVYYGFYKKALELFNTYREKVEKRWWFEMGMMVCLRANHIRQFDKLLEHSLKTFGSDYISPKVLRTAIRKKLYLRNYQCSEKLTKLLINVASAYGLKLSSQQQQNPQGIFFQSETQADLFLNEKEQVTEADFVAIIQYHLFRNREKPALALLAEFVKLPDVDKNLLSTALVHLKLHLLKSFDVLEKDMHSLLSGKDMKLLRQAFSNATRRFHVEAIQKKFDTVLFDSISDIASQPVLTQNIEQFVANTNKNLPRNPENIQDETVHSKKLHNLLKALLKSGKEDLAASLMKDANSYSSSVKESKLSLDEDDFSGINAHHYAVWIDHYSRKVQKAKNPREKYEYEAKIKEIVEKVNKGNVQYNSVLMTSLLTHFTVTANLDNCFNIINPLMDFRCSKTEESKGNRKLLSFFERREITKSLYFQIWKALILYFSRFENGLGTTEIRSNQKAWGYKISAERSKITVHPNYDFRTLFKLMVLEDNILPDRPFYHTILQAFIKVRDWAYIPAILRYMRHINGIEIDRKLQRYINGGLKLEYIAVERERLDSQKAEFSGVEKFTTPVVNTARRNVENRIKKGDILGETSTDYSLQVEQNILNFLRSYRSHEMERVQQAFEEVGIEVINT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDN6</accession>
    <name>C5DDN6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein YAE1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Protein yae1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0C02464g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the YAE1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552335.1">
        <property type="nucleotide sequence ID" value="XM_002552289.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDN6"/>
    <dbReference type="FunCoup" id="C5DDN6">
        <property type="interactions" value="9"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDN6"/>
    <dbReference type="GeneID" id="8291195"/>
    <dbReference type="KEGG" id="lth:KLTH0C02464g"/>
    <dbReference type="eggNOG" id="KOG4774">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066684_2_0_1"/>
    <dbReference type="InParanoid" id="C5DDN6"/>
    <dbReference type="OMA" id="CKNNEAP"/>
    <dbReference type="OrthoDB" id="20086at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019191">
        <property type="entry name" value="Essential_protein_Yae1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038881">
        <property type="entry name" value="Yae1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18829">
        <property type="entry name" value="PROTEIN YAE1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18829:SF0">
        <property type="entry name" value="PROTEIN YAE1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09811">
        <property type="entry name" value="Yae1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Essential protein Yae1 N-terminal" evidence="9">
        <location>
            <begin position="55"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF09811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR21897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="157" mass="17669" checksum="8B9073E3E5F4FBF9" modified="2009-07-28" version="1">MPSDKKRTSRQVISMSMSSSDDDIWGSESDGAPEAIGSTETVEVRKLRLIHSKRGYLDGISSSKEENLQKGFDDSFEQGAVLGKRVGKVLGGLYILGMLHGRFDEKLTDDLMEAQKELKINKVLSTKHFDENCNLLGVPNPIDKWEKILTEHQKRYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDP2</accession>
    <name>C5DDP2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0C02596p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0C02596g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552341.1">
        <property type="nucleotide sequence ID" value="XM_002552295.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDP2"/>
    <dbReference type="FunCoup" id="C5DDP2">
        <property type="interactions" value="36"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDP2"/>
    <dbReference type="GeneID" id="8291201"/>
    <dbReference type="KEGG" id="lth:KLTH0C02596g"/>
    <dbReference type="eggNOG" id="ENOG502QVBD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009854_1_1_1"/>
    <dbReference type="InParanoid" id="C5DDP2"/>
    <dbReference type="OMA" id="VKILCWD"/>
    <dbReference type="OrthoDB" id="201515at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008418">
        <property type="term" value="F:protein-N-terminal asparagine amidohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070773">
        <property type="term" value="F:protein-N-terminal glutamine amidohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07566">
        <property type="entry name" value="ScNTA1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039703">
        <property type="entry name" value="Nta1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11750">
        <property type="entry name" value="PROTEIN N-TERMINAL AMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11750:SF26">
        <property type="entry name" value="PROTEIN N-TERMINAL AMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="1">
        <location>
            <begin position="10"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="415" mass="46613" checksum="A20371B95A35DEC5" modified="2009-07-28" version="1">MGLKRLTVNLRIAVVQLNPQIGHLEETVVRAHSLVRRVERYQPDIVVFPEFALSGYSFHSRAEISAHLCRPQEGPAWEFCQQISRKLSCVTVMGYPERGDNQQAYNSALVVDEEGKLAFNYRKSFLYDTDEEWQCAENPAGFQAFELPLKGKARDHDGHVHDVKLRSAIGICMDLSPYKFKAPFQECEFSTYQLEHGTELLIVPMAWLHASSVTRDTADPATGKAEIARTMDQLGLPLHGSQNQFQVNIKLRDGTCEEECPGNGTLTSAPSDAGVASSYSDLSRPDQQNLDYWTIRFMPFLACKWRENWFVEKALKPILAKFKGSRFTYLGSSLKAPWLFENKNALLVLCNRCGAEDGATVYAGSSGFLKFNGQYGNEEEILNSSNRSVELLGNLGKGYEGVIVRDVSFEIERDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDV4</accession>
    <name>C5DDV4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0C04070p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0C04070g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552403.1">
        <property type="nucleotide sequence ID" value="XM_002552357.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDV4"/>
    <dbReference type="FunCoup" id="C5DDV4">
        <property type="interactions" value="49"/>
    </dbReference>
    <dbReference type="GeneID" id="8291267"/>
    <dbReference type="KEGG" id="lth:KLTH0C04070g"/>
    <dbReference type="eggNOG" id="ENOG502S3ZA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2145549_0_0_1"/>
    <dbReference type="InParanoid" id="C5DDV4"/>
    <dbReference type="OMA" id="HIKYEIT"/>
    <dbReference type="OrthoDB" id="4035606at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.250">
        <property type="entry name" value="YML108W-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015080">
        <property type="entry name" value="DUF1892"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035946">
        <property type="entry name" value="YML108W-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08987">
        <property type="entry name" value="DUF1892"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89975">
        <property type="entry name" value="Hypothetical protein Yml108w"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR21965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="113" mass="12956" checksum="1D8A9A45FA9FCA89" modified="2009-07-28" version="1">MSDERNYRMLVLVEDVLEQAGEKNEQETKGEGAPKATHEFVDELILPFEIDDMDKLNEWFDKFDADICIPNEGFIKYEISSDGLVVLLLDRSKEDVVAQVRKFVEAESGDEIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDV7</accession>
    <name>C5DDV7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">orotate phosphoribosyltransferase</fullName>
            <ecNumber evidence="5">2.4.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0C04158g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.10" evidence="5"/>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552406.1">
        <property type="nucleotide sequence ID" value="XM_002552360.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDV7"/>
    <dbReference type="FunCoup" id="C5DDV7">
        <property type="interactions" value="234"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDV7"/>
    <dbReference type="GeneID" id="8291271"/>
    <dbReference type="KEGG" id="lth:KLTH0C04158g"/>
    <dbReference type="eggNOG" id="KOG1377">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074878_0_1_1"/>
    <dbReference type="InParanoid" id="C5DDV7"/>
    <dbReference type="OMA" id="SPFFMNA"/>
    <dbReference type="OrthoDB" id="5553476at2759"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00119"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004588">
        <property type="term" value="F:orotate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046132">
        <property type="term" value="P:pyrimidine ribonucleoside biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000008">
        <property type="entry name" value="Orotate phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01208">
        <property type="entry name" value="PyrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023031">
        <property type="entry name" value="OPRT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004467">
        <property type="entry name" value="Or_phspho_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00336">
        <property type="entry name" value="pyrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46683">
        <property type="entry name" value="OROTATE PHOSPHORIBOSYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46683:SF1">
        <property type="entry name" value="OROTATE PHOSPHORIBOSYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="9">
        <location>
            <begin position="47"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR21968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="224" mass="24424" checksum="5FB4A60CB9D62EEC" modified="2009-07-28" version="1">MPAVLEDYQKNFLDLALESQALKFGTFTLKSGRQSPYFFNLGQFNTGKLLSNLATAYAIAIIQSDLKFDVVFGPAYKGIPLAAIVCVKLAEIGGTKFQNVQYSFNRKEAKDHGEGGTIVGASLEEQRVLIIDDVMTAGTAINEAFQIIAQNKGNVVGTIVSLDRQEVLSTESKEPLSATQAVTQKHGIPVLSIVTLADIISYLDGKIPAEEKRKMEEYLQIYGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDW8</accession>
    <name>C5DDW8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Coupling of ubiquitin conjugation to ER degradation protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0C04400g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the CUE1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552417.1">
        <property type="nucleotide sequence ID" value="XM_002552371.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDW8"/>
    <dbReference type="FunCoup" id="C5DDW8">
        <property type="interactions" value="119"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDW8"/>
    <dbReference type="GeneID" id="8291282"/>
    <dbReference type="KEGG" id="lth:KLTH0C04400g"/>
    <dbReference type="eggNOG" id="ENOG502S35Z">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115919_0_0_1"/>
    <dbReference type="InParanoid" id="C5DDW8"/>
    <dbReference type="OMA" id="TVNRFME"/>
    <dbReference type="OrthoDB" id="3824970at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14424">
        <property type="entry name" value="CUE_Cue1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000050">
        <property type="entry name" value="Related to AMFR protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.4310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003892">
        <property type="entry name" value="CUE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041158">
        <property type="entry name" value="Cue1_U7BR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02845">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18499">
        <property type="entry name" value="Cue1_U7BR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00546">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51140">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="domain" description="CUE" evidence="11">
        <location>
            <begin position="61"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="34"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="106"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="37"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="118"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00061383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00072899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR21979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="194" mass="22041" checksum="1BADBFB10425240C" modified="2009-07-28" version="1">MDSYSLTFFVIVIATFISVKWLLKSESHPSAQRAEASVSATSSSRSTNQSSSTRRRKRRTVTDNMIEVVQSLAPNLHIEQIRYNLEQTGSVEETVEAFLRGDEFSFPPGYTPEVPEPTSHQNTNGSETADPRKKSNIHPDNLLAKYKVDPSEDMSNVDYLSLSIEERKRFLVWQARRDMARTLQQDETLSALLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5DE05</accession>
    <name>C5DE05_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">KLTH0C05258p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0C05258g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552454.1">
        <property type="nucleotide sequence ID" value="XM_002552408.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DE05"/>
    <dbReference type="FunCoup" id="C5DE05">
        <property type="interactions" value="15"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DE05"/>
    <dbReference type="GeneID" id="8291321"/>
    <dbReference type="KEGG" id="lth:KLTH0C05258g"/>
    <dbReference type="eggNOG" id="KOG0800">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076142_1_0_1"/>
    <dbReference type="InParanoid" id="C5DE05"/>
    <dbReference type="OMA" id="EEDWGMY"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45768">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF13-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45768:SF18">
        <property type="entry name" value="RING-H2 FINGER PROTEIN ATL47-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="12">
        <location>
            <begin position="83"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR22016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="152" mass="17192" checksum="01548E50848F76DE" modified="2009-07-28" version="1">MSTYEEEHNTSGNSNSRRDLRAQIRNLYQEDGSSTALLQLLAQLIPESMQEELLNEKEEGCPETFIDSLPRVNKCDLKGADACSICCCGYLDDTHPLVVKLPHCSHRFDLECVAVWLSKSRTCPLCRDDVLSHKPKVDISNVELEEDWGMYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5DE46</accession>
    <name>C5DE46_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0C06204p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0C06204g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the APC3/CDC27 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552495.1">
        <property type="nucleotide sequence ID" value="XM_002552449.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DE46"/>
    <dbReference type="FunCoup" id="C5DE46">
        <property type="interactions" value="981"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DE46"/>
    <dbReference type="GeneID" id="8291363"/>
    <dbReference type="KEGG" id="lth:KLTH0C06204g"/>
    <dbReference type="eggNOG" id="KOG1126">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008850_2_0_1"/>
    <dbReference type="InParanoid" id="C5DE46"/>
    <dbReference type="OMA" id="CKYDCYK"/>
    <dbReference type="OrthoDB" id="329563at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007091">
        <property type="term" value="P:metaphase/anaphase transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558">
        <property type="entry name" value="CELL DIVISION CYCLE 16,23,27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558:SF13">
        <property type="entry name" value="CELL DIVISION CYCLE PROTEIN 27 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12895">
        <property type="entry name" value="ANAPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="136"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="409"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="443"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="477"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="545"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="579"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="231"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="246"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="261"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR22057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="627" mass="70267" checksum="6F642AAAA7992091" modified="2009-07-28" version="1">MFRVGSGGLIRASDENCERLKGLIERSVEQNNLETAEFLAELLCSETGRLARGARERLEAAYQYGLVLYLRGDFKSAQDVVRPFKCESMLCAYVLARCALKLDQSSWHEGARALEALAETYSIQNAEPQFGMPEEATVFSVLGQLYAKMGKIKESCAAHSRALELDSYLWESFKALCDMRANVRVGKLYKTAAGSPKTQPAQQHRNRKGLVLKPHTPFKVPAFDSKRRAYQAQPASAMRHRPPAPTSSLLKTTNGAGVTATPTSSFSKSKLLSTPPSQFSISEKPVTPEDIENNLVSAQALGLDAIFYALAKSYKSACRYDCYKAVRILNELPQHILTAMPWVLSALGKLHFELVNYEMSKKYFAALRELQPFRIPDMEIFSTLLWHLNDTTGLSHLCHELLEVNRLAPETWCCIGNLFSLKKDHEESIKAFQRSTQLDPSFTYAYTLQGHEYSSNDAFDTAMTCYRKALGSNPQHYNAHYGLGMCCLKLGQYEECLLHFEKARSINPVNVILICCCGVALEKLSHQEKALQYYDLACELQPVSSLALFKRAQLLLAMGKYNFALENFEKLVTIAPDEATIHFLLGQLYQIVGRKQDAVKELTIAMNLDPKGSQLIKSALEKCHQLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DE72</accession>
    <name>C5DE72_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0C06820p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0C06820g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552521.1">
        <property type="nucleotide sequence ID" value="XM_002552475.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DE72"/>
    <dbReference type="FunCoup" id="C5DE72">
        <property type="interactions" value="103"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DE72"/>
    <dbReference type="GeneID" id="8291390"/>
    <dbReference type="KEGG" id="lth:KLTH0C06820g"/>
    <dbReference type="eggNOG" id="KOG1550">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_126_4_1"/>
    <dbReference type="InParanoid" id="C5DE72"/>
    <dbReference type="OMA" id="HFTIESK"/>
    <dbReference type="OrthoDB" id="272077at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000707">
        <property type="entry name" value="Chitin synthase regulatory factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051726">
        <property type="entry name" value="Chitin_Synth_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006597">
        <property type="entry name" value="Sel1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46430:SF1">
        <property type="entry name" value="CHITIN SYNTHASE REGULATOR SKT5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46430">
        <property type="entry name" value="PROTEIN SKT5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08238">
        <property type="entry name" value="Sel1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00671">
        <property type="entry name" value="SEL1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="533"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="39"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="76"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="545"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="561"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="620"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="636"/>
            <end position="648"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR22083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="648" mass="70706" checksum="3D8F13DCB19ADFEC" modified="2009-07-28" version="1">MAAATHPYRQNLGTVTPVTLAFKEQGDTESGDTSQTSSELKKLIDEPDHPEDPRRILEGLPSPQGVRGPLLQHNKSASSPASSLTGTPSSSRSQLQGSGASPSLELRGDGHAKRRPKSLDLSHLFLLSDKDTQFTSTNESAADLSHKMITEYLGEAHNSTLVPRLKTIEMYKQNVKKSKDPNVLFQYAQYMLQTALTIDPLAGQDETTKKEEASQEEMRKQFLKEAQHYLKKLSVKGYSDAQYLLGDAYSSGAFGKVNNKDAFVLFQSAAKHGHVESAYRTAHCFEEGFGTTRDSRRAVEFLKFAASRNHPSAMFKLGLYSFYGRMGLPHDVNTKQNGIKWLSRAAARANELTNAAPYELAKIYQNGFLDIIIPDTKYAMELYIQAASLGHALSSTALGQIYEAGNEIVPADTSLSVHYYTQAAMQGEPSAMLGLCAWYLMGAEPAFKKDEEEAFQWALRAAKAGLPKAQFTIGYFYEKGKGCEPDAASARKWYERAAKNQDPRAISKLEGEGRVLRKQKSISTLNLFSNNSSSNLLQTDDRSRSGLSPNSSFSQTGRQPSEAYTSNASNTSTSRKVSANGRSDLGAGSETKNAAGPAGTLDLTDAVVTSTKKKGKSKKTTASSTLPTSQGDKKGQSKKKKDKKCVIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEM0</accession>
    <name>C5DEM0_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">glutamine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Glutaminyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">KLTH0C10340g</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>tRNA(Gln) + L-glutamine + ATP = L-glutaminyl-tRNA(Gln) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:20121"/>
            <dbReference type="Rhea" id="RHEA-COMP:9662"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.18"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.18" evidence="2"/>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552669.1">
        <property type="nucleotide sequence ID" value="XM_002552623.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEM0"/>
    <dbReference type="FunCoup" id="C5DEM0">
        <property type="interactions" value="1343"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEM0"/>
    <dbReference type="GeneID" id="8291549"/>
    <dbReference type="KEGG" id="lth:KLTH0C10340g"/>
    <dbReference type="eggNOG" id="KOG1148">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001882_2_3_1"/>
    <dbReference type="InParanoid" id="C5DEM0"/>
    <dbReference type="OMA" id="TWCIYPM"/>
    <dbReference type="OrthoDB" id="10250478at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004819">
        <property type="term" value="F:glutamine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006425">
        <property type="term" value="P:glutaminyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00807">
        <property type="entry name" value="GlnRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.240.10:FF:000007">
        <property type="entry name" value="Glutamine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2420:FF:000001">
        <property type="entry name" value="Glutamine--tRNA ligase cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.1290:FF:000002">
        <property type="entry name" value="Glutamine--tRNA ligase cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.240.10:FF:000015">
        <property type="entry name" value="Glutaminyl-tRNA synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000183">
        <property type="entry name" value="Glutaminyl-tRNA synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.1290">
        <property type="entry name" value="Glutaminyl-tRNA synthetase, non-specific RNA binding region part 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.240.10">
        <property type="entry name" value="Ribosomal Protein L25, Chain P"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004514">
        <property type="entry name" value="Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007638">
        <property type="entry name" value="Gln-tRNA-synth_Ib_RNA-bd_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007639">
        <property type="entry name" value="Gln-tRNA-synth_Ib_RNA-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042558">
        <property type="entry name" value="Gln-tRNA-synth_Ib_RNA-bd_N_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042559">
        <property type="entry name" value="Gln-tRNA-synth_Ib_RNA-bd_N_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050132">
        <property type="entry name" value="Gln/Glu-tRNA_Ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000924">
        <property type="entry name" value="Glu/Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020058">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020059">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_codon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020056">
        <property type="entry name" value="Rbsml_bL25/Gln-tRNA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011035">
        <property type="entry name" value="Ribosomal_bL25/Gln-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049437">
        <property type="entry name" value="tRNA-synt_1c_C2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00440">
        <property type="entry name" value="glnS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43097:SF4">
        <property type="entry name" value="GLUTAMINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43097">
        <property type="entry name" value="GLUTAMINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00749">
        <property type="entry name" value="tRNA-synt_1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03950">
        <property type="entry name" value="tRNA-synt_1c_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20974">
        <property type="entry name" value="tRNA-synt_1c_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04558">
        <property type="entry name" value="tRNA_synt_1c_R1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04557">
        <property type="entry name" value="tRNA_synt_1c_R2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00987">
        <property type="entry name" value="TRNASYNTHGLU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50715">
        <property type="entry name" value="Ribosomal protein L25-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutaminyl-tRNA synthetase class Ib non-specific RNA-binding" evidence="15">
        <location>
            <begin position="5"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="Glutaminyl-tRNA synthetase class Ib non-specific RNA-binding" evidence="14">
        <location>
            <begin position="166"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamyl/glutaminyl-tRNA synthetase class Ib catalytic" evidence="12">
        <location>
            <begin position="246"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamyl/glutaminyl-tRNA synthetase class Ib anti-codon binding" evidence="13">
        <location>
            <begin position="563"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA synthetases class I (E and Q) anti-codon binding" evidence="16">
        <location>
            <begin position="677"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="186"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="200"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF03950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF04557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF04558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF20974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAR22231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="800" mass="91352" checksum="368BE0388795A91D" modified="2009-07-28" version="1">MSSVEELSKLFSEIGFEEPKVKEIVKNKKVSGSLESLINECPNNYEWDKSSRTLMHQLASLIKGTELPNQKLIVNGILSGDLKTPLQVDAAFKYIKDAKEDATEENLKKEAGVGIVVTESQVREGVQKYISDHRDDIVTQRYKLVPGILANVKGLPELKWAEPRLFKPIIDEEVLKLLGPKDERDVVKKEKKKKGKEAKGGAPAETAGPKRTMFTEGFLGDLHKVGENPQAYPELMADHLKVTGGKVRTRFPPEPNGYLHIGHSKAIMVNFGYAKHNDGVCYLRFDDTNPEAEAPEYFESIKSMVSWLGFKPWKITYSSDYFDKLYELAEKLITNGKAYVCHCTAEEIKRGRGIKEDGTPGGERHACVHRGRSVEENLAEFRKMRDGAYKPGEATLRMKQDLESPSPQMWDLIAYRVLNASHPRTGDKWKIYPTYDFTHCLVDSFENITHSLCTTEFYLSRESYEWLCDQVHVFRPAQREYGRLNITGTILSKRKIAKLVNEHYVRGWNDPRLFTLEAIRRRGVPPGAILSFINTLGVTTSTTNIQVVRFESAIRKYLEDNTPRLMMVLDPVEVVVDNLPEDYEELVTVPYRPGTPEFGERQVPFTGKFYIERSDFSEATDDKEFFRLTPNQPVGLIKVPYTVSYQSVDKDSNGKVIRIHVKYDNEDAKPKKPKTYIQWVPVSSKYNSPVRIAETRVYNQLFKSENPSAHPEGFLNDINPESELVHGNSVVEHNFNHVIEKSPWVVDSAKESEFYVEEQHGRKEVCRFQAMRVGYFTLDLESESDKIILNRIVSLKDSTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEN4</accession>
    <name>C5DEN4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9">Lysophospholipase</fullName>
            <ecNumber evidence="2 9">3.1.1.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0C10670g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 1-acyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15177"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.5"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the lysophospholipase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.5" evidence="2 9"/>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552683.1">
        <property type="nucleotide sequence ID" value="XM_002552637.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEN4"/>
    <dbReference type="FunCoup" id="C5DEN4">
        <property type="interactions" value="84"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEN4"/>
    <dbReference type="GeneID" id="8291564"/>
    <dbReference type="KEGG" id="lth:KLTH0C10670g"/>
    <dbReference type="eggNOG" id="KOG1325">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014602_0_1_1"/>
    <dbReference type="InParanoid" id="C5DEN4"/>
    <dbReference type="OMA" id="FGHINMS"/>
    <dbReference type="OrthoDB" id="4084751at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046475">
        <property type="term" value="P:glycerophospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07203">
        <property type="entry name" value="cPLA2_Fungal_PLB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1090.10:FF:000010">
        <property type="entry name" value="Lysophospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002642">
        <property type="entry name" value="LysoPLipase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728">
        <property type="entry name" value="CYTOSOLIC PHOSPHOLIPASE A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728:SF33">
        <property type="entry name" value="LYSOPHOSPHOLIPASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01735">
        <property type="entry name" value="PLA2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00022">
        <property type="entry name" value="PLAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51210">
        <property type="entry name" value="PLA2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 8 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 8 9" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="6 8 9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005125149" description="Lysophospholipase" evidence="9">
        <location>
            <begin position="18"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="domain" description="PLA2c" evidence="10">
        <location>
            <begin position="30"/>
            <end position="579"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR22245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="652" mass="70860" checksum="9529B8A42D4B939E" modified="2009-07-28" version="1">MLWLVPLFLLALRAVQAWSPTDSYAPGNVSCADDISLVREASGLSPNETAWLEKRDAYTKQALREFLDGATANFSNSSLVDSLWSADKIPRVAMAFSGGGYRAMLSGAGMISAFDNRTNGSTEHGLGGLLQGATYLAGLSGGNWLVGSLAWNNWTSVQHVIDTRGKDDELWDISNSIVNPGGINILSSASRWDQISDAVEAKQDAGFNTSLADVWGRALAYNFFPTLSKGGDGYTWSTLRDADVFKNGEMPFPISVADGRYPGTQIVDLNSTLFEFNPFEMGSWDPSLNAFTDVKYLGTNARNGTPVTKGQCVAGFDNTGFVIGTSSTLFNQFLLQLNTTSVSSFLKGIIGDFLKDLSEDYDDIAIYNPNPFRETEFVESNYTKGIVNSENLFLVDGGEDGENIPFVPLIQKERDVDVIFALDNSADTDEYWPAGLSLISTYERQFGAQGKDIAFPYVPDFDTFINEGLNQKPVFFGCDASNTTGLSYTPPLIVYMPNNRQSYNSNTSTFKMSYSSSQRMKMFQNGFEAVTRNNLTDDSSFRGCVACAVLRRKQESQNMTLPQECAKCFQDYCWNGKISNKTAKVSTIENYSGSISFITDSASADAASSTAAASSSGTSTSKKNAAASLPSSNSLKWYVAFASLLGMAIGLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEQ3</accession>
    <name>C5DEQ3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Alpha-1,3-glucosyltransferase</fullName>
            <ecNumber evidence="10">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0C11176g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2 10">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Endoplasmic reticulum membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the ALG6/ALG8 glucosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="10"/>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552702.1">
        <property type="nucleotide sequence ID" value="XM_002552656.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEQ3"/>
    <dbReference type="FunCoup" id="C5DEQ3">
        <property type="interactions" value="1006"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEQ3"/>
    <dbReference type="GeneID" id="8291587"/>
    <dbReference type="KEGG" id="lth:KLTH0C11176g"/>
    <dbReference type="eggNOG" id="KOG2575">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008110_2_1_1"/>
    <dbReference type="InParanoid" id="C5DEQ3"/>
    <dbReference type="OMA" id="FQVPPMH"/>
    <dbReference type="OrthoDB" id="5589195at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042281">
        <property type="term" value="F:dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004856">
        <property type="entry name" value="Glyco_trans_ALG6/ALG8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12413">
        <property type="entry name" value="DOLICHYL GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12413:SF1">
        <property type="entry name" value="DOLICHYL PYROPHOSPHATE MAN9GLCNAC2 ALPHA-1,3-GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03155">
        <property type="entry name" value="Alg6_Alg8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="133"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="167"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="215"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="252"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="326"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="351"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="417"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="469"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="501"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR22264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="536" mass="61221" checksum="AEFC7BC42F5C33AB" modified="2009-07-28" version="1">MDARKSLPKESFFASPLYDFLSPFRPVANQYYAEYVIVIFALVIRCAVGLGSFSGEATVPMHGDFEAQRHWMELTTNLPLSQWYWFDLQYWGLDYPPLTAYHSYALGKLGSVLNPEWFALGSSRGNETPELKAYMRFTVILSEAALYIPAVLYFTKWFGIKRRQSPIGQYIAAAAILFQPSLILIDHGHFQYNSVMLGLTVYAINNLLDGFHGPAAICFMLSLCFKQMALFYSPIFFAYLLSRSLFSPSFKFSRFMSVALSTVITFCLMFSPLYVFGGMKNVLQSIHRIFPFSRGIFEDKVANFWCVSNVIVKYADNFTQDQLQKLSTFATAAGFLPSFALVVLYPKKHMIPYGLAACSMAFFLFGFQVHEKSILVPLLPISLLFCSTDWNVLAIVSWINNVALFTLWPLLKKDKLALQYGIMMLLSNWLIGNFSFVTPRFLPGFITPGPSMSNVAETYRRRSFLPNNIVWKILIITSYIVMAVIHILDLFVQPPAKLPDLWVLMNCALGFTCFSVFWLWNNYKLFTMRNKSILDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-04-02" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>C5DET1</accession>
    <name>C5DET1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0C11924p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0C11924g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552730.1">
        <property type="nucleotide sequence ID" value="XM_002552684.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DET1"/>
    <dbReference type="GeneID" id="8291621"/>
    <dbReference type="KEGG" id="lth:KLTH0C11924g"/>
    <dbReference type="eggNOG" id="ENOG502QPQC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_582736_0_0_1"/>
    <dbReference type="InParanoid" id="C5DET1"/>
    <dbReference type="OMA" id="YGPNPTT"/>
    <dbReference type="OrthoDB" id="4070743at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000128">
        <property type="term" value="P:flocculation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001389">
        <property type="entry name" value="Flocculin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00624">
        <property type="entry name" value="Flocculin"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="325"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="394"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="331"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="421"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="469" mass="48365" checksum="17C04E08EE8F5AA0" modified="2009-07-28" version="1">MASEPQVLCITMHTPTSTYSTASTAFSIWTGSETSTYSTAVSTFTGSDGSTTTETVYYVEAPTVATTVFTPGTCSETSTYSVGRTAVTGSDGQPTTETMHYIETPAAVTTAYTAGYVTAATMYSTGATTFPGSDVKPTIQTAYYVETPSSGSQVYTQWTSSFASTYSTEFAAVVVDSVATTKTICHVETPSSGSDVYTPWTGSFSSTYSTDLSTFTGSGGQPTTNTIYYVEAPSSGSDVYTPWSGSFTSTFSTEFSTHTSSDGRPIIDTIYRVETLILASSVLARWTDKFTSSYSTKYATFTGSDGLASTQTAYYIETPVSAYNGNSTSYTNSPESTSTSMTSSGPPATTTSNAATTHDLPTSSQLSPGSISISSTATGAGFGSSSVSNAGFGSSCNSGSGSNSGSGSGSSSRLSQTTTITPKTQSSSSVYSTSSSETGSTASPYVSTYEGAASRIGFSLLFVLPLAFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEZ6</accession>
    <name>C5DEZ6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">KLTH0D11044g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553189.1">
        <property type="nucleotide sequence ID" value="XM_002553143.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEZ6"/>
    <dbReference type="FunCoup" id="C5DEZ6">
        <property type="interactions" value="261"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEZ6"/>
    <dbReference type="GeneID" id="8295429"/>
    <dbReference type="KEGG" id="lth:KLTH0D11044g"/>
    <dbReference type="eggNOG" id="KOG0583">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011780_1_0_1"/>
    <dbReference type="InParanoid" id="C5DEZ6"/>
    <dbReference type="OMA" id="FYHRQFD"/>
    <dbReference type="OrthoDB" id="942095at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14003">
        <property type="entry name" value="STKc_AMPK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000650">
        <property type="entry name" value="Serine/threonine-protein kinase ppk16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 11 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 11 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="37"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="379"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="462"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="699"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="823"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="410"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="464"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="511"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="532"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="550"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="578"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="599"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="631"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="701"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="724"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="839"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11 13">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="157"/>
            <end position="158"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="12">
        <location>
            <position position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAR22751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="849" mass="95620" checksum="840AB469E9C41ADF" modified="2009-07-28" version="1">MFPSRLEQNQLKANISASYNKLYGQFSLTELRDVGNYRILKIVGEGSFGKVYLASHRLTHQKVVLKMGSKNDPNVVREVFYHRQFDYVYITKLYEVIVTENSVWMALEYCPGKELYEYLLIKKRIPLDECSHLFSQIVGAVFYAHSMKCVHRDLKLENILLDKKGQAKLTDFGFTRECATKGILETVCGTTVYMAPELIERKPYEGYKIDTWSLGIILYTMIHGAMPFDEEDDIKTKYRIVNNEPFYDDNFIDANGKDLIMQLLQKDPSQRPSLTQVLQHPFMFPHGSSILEKTDKTIKRQSQGCKHFHSKLERRLLKKLKQSGFDTQAIKQSVNKKKCDALAGTWQLLLEKEKRSEILRHPKRSRSVLSVKKVFEPSLPSRKGSEDLKNPEQGGKAASLKRIMSRKSDTVSIRQPINATQLKMSPQQSPAEQTEGSGNTRSNKKHNFFNKMTNFFKTKKQNGIHYQNGSPSLSSKNADKRKRNTNSFESNKNSPHRSPTRNGNGLMPGIDVEKKPHEKLKIEEPMLKKFKSNTSSEGSRQTSMGNYDSELDHRPANNEHEVPEGNKLLEQRGRPMSILSQQSEMSNDTYNSEYSTDGNISNSKANSSISSNNRNISSVILSPTGDLTDNQTTQSRVTTRRSMSIMSSASSASERSSRTDSFYDISTASSPKTMDLRAMGNYSNADTMVPRMTVGSQWLSKRGRSPVGRRGRPPKRNISRKLMNHSSSAAQSVIQEESSFDNEDDNSGALIEETEGQGIPDAPLLHDLETPSSISGTPSKPSTPICAEIQPTDTRPVLFAMGRSFSGGSRWSRNLSEERILTSDFPSLQNAGVHSDIGSADDEDNSLDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>C5DF23</accession>
    <name>C5DF23_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0D11638p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0D11638g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553216.1">
        <property type="nucleotide sequence ID" value="XM_002553170.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DF23"/>
    <dbReference type="FunCoup" id="C5DF23">
        <property type="interactions" value="82"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DF23"/>
    <dbReference type="GeneID" id="8295456"/>
    <dbReference type="KEGG" id="lth:KLTH0D11638g"/>
    <dbReference type="eggNOG" id="KOG2084">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038964_1_0_1"/>
    <dbReference type="InParanoid" id="C5DF23"/>
    <dbReference type="OMA" id="FRKEVCH"/>
    <dbReference type="OrthoDB" id="1028014at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20071">
        <property type="entry name" value="SET_SMYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050869">
        <property type="entry name" value="H3K4_H4K5_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12197">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SMYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12197:SF294">
        <property type="entry name" value="POTENTIAL PROTEIN LYSINE METHYLTRANSFERASE SET6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SET" evidence="1">
        <location>
            <begin position="12"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="353" mass="41191" checksum="463385B9E969AE41" modified="2009-07-28" version="1">MMPIEEAVYISELFDIRTTDNSGRACFASKDLKKGTDVLCLTKCLGASTCYEFRKEVCSQCFQYDGGQRMKVRLNNKFLKGAGLWFCTENCRDQFLRQEHVDHLIDALETLLVSWEIQAKKRIGSIDRASNSSISTEKIESLWQDIRDNWITRIDRMKPTKRVLQLPIINEDEYVCARFVIHCLFTLKTTDTSSLHMAAFRNLQSNESSKISRFPVLLDLQKRVFQTLYILLPPFLKSGFDIPTFRHILGSEYGNSFGIWQNEEASDSREYLGYWVLPEASFFNHSCAPNLIKHRFGNKMIFTLNSDVTKDQELCIDYKDILDLKVDERRHILKENWFFSCECPRCKIEMQIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>C5DF38</accession>
    <name>C5DF38_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0D11968p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0D11968g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553231.1">
        <property type="nucleotide sequence ID" value="XM_002553185.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DF38"/>
    <dbReference type="FunCoup" id="C5DF38">
        <property type="interactions" value="73"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DF38"/>
    <dbReference type="GeneID" id="8295471"/>
    <dbReference type="KEGG" id="lth:KLTH0D11968g"/>
    <dbReference type="eggNOG" id="KOG0562">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066882_3_1_1"/>
    <dbReference type="InParanoid" id="C5DF38"/>
    <dbReference type="OMA" id="IHDMFPK"/>
    <dbReference type="OrthoDB" id="3512845at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033699">
        <property type="term" value="F:DNA 5'-adenosine monophosphate hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990165">
        <property type="term" value="F:single-strand break-containing DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000012">
        <property type="term" value="P:single strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032566">
        <property type="entry name" value="Znf-C2HE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12486:SF4">
        <property type="entry name" value="APRATAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12486">
        <property type="entry name" value="APRATAXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11969">
        <property type="entry name" value="DcpS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16278">
        <property type="entry name" value="zf-C2HE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aprataxin C2HE/C2H2/C2HC zinc finger" evidence="1">
        <location>
            <begin position="150"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="213" mass="25657" checksum="4753C8572111ADAA" modified="2009-07-28" version="1">MSFRYVLKNYIVEPQLYPDEVLFFDSHAVIIKDAFPKSQFHFLILPRDLKITWTDPTSLSAEQKSKLQGYVDWALTRAFEDFTKSYTFSKEGMIPFEKKYQFEDKQFFIDKFTQVGIHSVPSLKNLHIHVMTKDFYSDRMKNKKHYNSFNTTFFVNWRRLPLEESPDATYLENEVIKKTDLKCCYCKKNFKNRFSELKLHLDREFKVRFTNQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5DF55</accession>
    <name>C5DF55_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein DML1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Protein dml1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0D12364g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the partitioning of the mitochondrial organelle and mitochondrial DNA (mtDNA) inheritance.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the misato family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553248.1">
        <property type="nucleotide sequence ID" value="XM_002553202.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DF55"/>
    <dbReference type="FunCoup" id="C5DF55">
        <property type="interactions" value="91"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DF55"/>
    <dbReference type="GeneID" id="8295488"/>
    <dbReference type="KEGG" id="lth:KLTH0D12364g"/>
    <dbReference type="eggNOG" id="KOG2530">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022511_2_2_1"/>
    <dbReference type="InParanoid" id="C5DF55"/>
    <dbReference type="OMA" id="DNGWGGF"/>
    <dbReference type="OrthoDB" id="271881at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049942">
        <property type="entry name" value="DML1/Misato"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029209">
        <property type="entry name" value="DML1/Misato_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019605">
        <property type="entry name" value="Misato_II_tubulin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13391">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION REGULATOR MISATO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13391:SF0">
        <property type="entry name" value="PROTEIN MISATO HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10644">
        <property type="entry name" value="Misat_Tub_SegII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14881">
        <property type="entry name" value="Tubulin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Misato Segment II tubulin-like" evidence="7">
        <location>
            <begin position="2"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="DML1/Misato tubulin" evidence="8">
        <location>
            <begin position="123"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF10644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF14881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR22810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="466" mass="53778" checksum="7B8EDBD64A24065C" modified="2009-07-28" version="1">MHEIISISASHRTNHVLTQFYNCQEEQLRDLDFENEPGVFLNPVVDRISKTVSYKPRALLWDAKTGNGALGAHQYSSTKDFFYGSENQRPKELLSQTSVKVIQTHEPTPKSEYQTALDTNNPAPPLSDQNSKYWSDYSKLIFGAPNLNTLDRWYHDPNNPSAPDFENLHQTYFDSYEVGFQEFATNYCSEFFDEKLRTQLEMCDELQGINLLSEIDNGWGGFSSSMLSNIRDELPKTDVMTWGFNQDDALSLGQPIHSTKTKFQKLCSKIRSTLSLVQESNLFFPIYSDPQLPLWTFAGRTCMLFDAVNACGSNRESEHRYTFNQMIGKITSGEPQRNIVSTLDIQGLDLSFYSRIPTYKGSSQKTYSHLSILRGKENQVADSFSINTYPWRPSDTIPEQFRSITNYKTNLAVTERPRDVFRHWEAMVSRYFRYDTDREELKEELGRLAETYEEGWYDDFDSGDDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DF98</accession>
    <name>C5DF98_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0D13376p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0D13376g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the OXA1/ALB3/YidC family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553291.1">
        <property type="nucleotide sequence ID" value="XM_002553245.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DF98"/>
    <dbReference type="FunCoup" id="C5DF98">
        <property type="interactions" value="79"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DF98"/>
    <dbReference type="GeneID" id="8295533"/>
    <dbReference type="KEGG" id="lth:KLTH0D13376g"/>
    <dbReference type="eggNOG" id="KOG1239">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029282_2_1_1"/>
    <dbReference type="InParanoid" id="C5DF98"/>
    <dbReference type="OMA" id="WQRKRIV"/>
    <dbReference type="OrthoDB" id="2148490at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032977">
        <property type="term" value="F:membrane insertase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032979">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane from matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001708">
        <property type="entry name" value="YidC/ALB3/OXA1/COX18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428:SF65">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX18, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428">
        <property type="entry name" value="OXA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR22853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="322" mass="35808" checksum="03DE9E90CEE766CE" modified="2009-07-28" version="1">MYALQAGSRCVSSRPHQRLRGAFVRPVNAVRTLSVFQEVTSSLVSLHEISGLPWLALVPLITFGLRTAFTLPLSVWQRKRIVKQQELRKVVQATTPVVKLRLAASTQANSKSAKAFEEEEGNVATTLQKPSLSPDQITLLALKETRSRQKKMFGESKVQLWKNALLPLVQIPLWVSVSMGLRELTKQRLVDSNLTHANPLQNIDSLDYVSRISSLDLSLPLDGLPILAPLLLGTLALLNVEHNGRVMTTTTSETMGIKLAPNPSSKVSQSMQSILNVSRLSCIFFMGVSSQAPLLLSAYWISSQLYSLVQNLLLDWLWPYQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFC4</accession>
    <name>C5DFC4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0D13992p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0D13992g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553317.1">
        <property type="nucleotide sequence ID" value="XM_002553271.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFC4"/>
    <dbReference type="STRING" id="559295.C5DFC4"/>
    <dbReference type="MEROPS" id="S09.A98"/>
    <dbReference type="GeneID" id="8295561"/>
    <dbReference type="KEGG" id="lth:KLTH0D13992g"/>
    <dbReference type="eggNOG" id="KOG4667">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_444876_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFC4"/>
    <dbReference type="OMA" id="RNYCYQK"/>
    <dbReference type="OrthoDB" id="449382at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026893">
        <property type="entry name" value="Tyr/Ser_Pase_IphP-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126:SF1">
        <property type="entry name" value="TYROSINE SPECIFIC PROTEIN PHOSPHATASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13350">
        <property type="entry name" value="Y_phosphatase3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR22879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="620" mass="70557" checksum="402917AB27DA4575" modified="2009-07-28" version="1">MVEQDIVQATVVKQQETSENERFLYLKHSEFHSSTGIASILTFPTSLKFPSGRERILAEAEFGFPQHKLVLLVHGHSAHKNTVYMPLLAQKLADMGYYVLRIDFRNMGDSEPNRDPKVGRTIAQDCEDLETVYQFAGSSQCQQLVGHCLVLDTIVAHSRGVMSMFEFARQRFVPNLINCCGRFVAWGLFEKSRLRNPNWDAEGGFKCEMLRYGKMQELWIPKPETVSAATVDTTKFADIDPRSWVMSIYGSKDSVIPLSAFGQYSNLFQGRHTAEMIMDADHNFYGLPGDPNAMNLPLRKGLINYNVTTVELISAHLSPEKQLQRFYETTKFIQDLSNPSQLSSRWPLPYSFSNVSNFRDIGGYKTRDGYRVKPKVLYRCANPSEVTDKGIKFMKEQLHVTRIFDLRSTKEAAENGVISGIDVENLAFNQNVSAAPEELARLYRGLLLSSYMFPEAYQVMMQNSIPQIRRFCRYIINEECDELHAAVFHCAAGKDRTGILAVLILGLLGVDDDTIAHDYEMTSLGLQTEKRLKKAMEDRGDRYYEMLGSKDLARDYQATPMSMCKNLLSSKYEAMRLFLDHFRSVYGSFDSFYSSVVKLSGSEIKKLKEALLEPQDIEID</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-04-02" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFE3</accession>
    <name>C5DFE3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0D14410p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0D14410g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553336.1">
        <property type="nucleotide sequence ID" value="XM_002553290.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFE3"/>
    <dbReference type="STRING" id="559295.C5DFE3"/>
    <dbReference type="GeneID" id="8295580"/>
    <dbReference type="KEGG" id="lth:KLTH0D14410g"/>
    <dbReference type="eggNOG" id="ENOG502QWIY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_540776_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFE3"/>
    <dbReference type="OMA" id="AYEVINF"/>
    <dbReference type="OrthoDB" id="3365616at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="CDD" id="cd22952">
        <property type="entry name" value="ART10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011021">
        <property type="entry name" value="Arrestin-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00339">
        <property type="entry name" value="Arrestin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arrestin-like N-terminal" evidence="1">
        <location>
            <begin position="19"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="416" mass="46738" checksum="7A4D8A4AC3B07365" modified="2009-07-28" version="1">MGPKLKLLVNKPANGEFHVSNEAITGKVVLELEKPAPIKELSVTMRGTSQTIVEMTPEAQNLIRPHFPMKSSHTLVNQKRQLFPPANVSKALDGPGKGFQVTKGSYSYEFELNIPHWLKCMGDHGPRGAGFNKDEEEPRLPPSFNSIASQAPANDPISFYHKMGTVTYYVKAQLILGKSMFRLTPSDPILEAYEVINFFPDPNKERSSAGRTQSFVSKKAVQFEKELSARLEVRAKRLQEVHRLDHLFKSGSRRLDRVYLVFETPPPPKFALSITRCRLQLVEVVNYLSAGHINAVTGLIPILDVPLDRQLDPSRIRCLENGTAEWPLDLSNNPELASYRFNEENLIFQGNKLFSFVCCNIRRAFKFHLTLTLSANGKSFEIDMSTTTSAISLASAEEHEPLPRYDVSDAPPVYLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFF2</accession>
    <name>C5DFF2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0D14608p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0D14608g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553345.1">
        <property type="nucleotide sequence ID" value="XM_002553299.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFF2"/>
    <dbReference type="GeneID" id="8295589"/>
    <dbReference type="KEGG" id="lth:KLTH0D14608g"/>
    <dbReference type="eggNOG" id="ENOG502S5RW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105160_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFF2"/>
    <dbReference type="OMA" id="THEDHIV"/>
    <dbReference type="OrthoDB" id="4058109at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR22907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="220" mass="25340" checksum="5F66A3144D0F89E0" modified="2009-07-28" version="1">MAQFVSNCSNALARLAFFRNSVQCSWRFGARREFGGTNRIPRKNWSKDAERIKVFGEQLDFSKYGVSCKEPQEVSKCLYPKKDCAVRMPDGQRFQVGHLQILGHSLLRVSVNKALLSVFKRSPQDISGFDFNYSDKMGHLSTSKRFISSLLRRYIRQRYEKLARIPMAESAVPLRVQKVFDQRSFHALIGYISLTNDREAVDRLLQEKLAKPIMKGVFGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFG9</accession>
    <name>C5DFG9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Copper transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0D15004g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the copper transporter (Ctr) (TC 1.A.56) family. SLC31A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553362.1">
        <property type="nucleotide sequence ID" value="XM_002553316.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFG9"/>
    <dbReference type="FunCoup" id="C5DFG9">
        <property type="interactions" value="43"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFG9"/>
    <dbReference type="GeneID" id="8295606"/>
    <dbReference type="KEGG" id="lth:KLTH0D15004g"/>
    <dbReference type="eggNOG" id="KOG3386">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079690_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFG9"/>
    <dbReference type="OMA" id="ENATVCC"/>
    <dbReference type="OrthoDB" id="161814at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005375">
        <property type="term" value="F:copper ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007274">
        <property type="entry name" value="Cop_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483:SF73">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN CTR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 31 COPPER TRANSPORTERS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04145">
        <property type="entry name" value="Ctr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0186">Copper</keyword>
    <keyword evidence="6" id="KW-0187">Copper transport</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR22924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="244" mass="27182" checksum="4388FFE2C567E81A" modified="2009-07-28" version="1">MSNYLDSDPLIKMAMGSSSSSKKTCKISMLWNWYTIDTCFIARSWRNDTKGKFAGSCIGCFLLVVAAQWLNRVSRQFDIELAKRRKLKVLAKYSNLPNSDDTSGLSLSDSAQQTLEQLLAEPDWKTSLKAVGTTLSHTWFLTSLKDEFAGLEGMASCCSTNIDVFPTLLDHILRSLVFVLQWGLSYIIMLLFMYYNGYIIISCLIGALIGRLLFNFEPLATCGGAGGSNTVAHDKEVNDRKCCM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-04-02" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>C5DG11</accession>
    <name>C5DG11_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0D01540p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0D01540g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552791.1">
        <property type="nucleotide sequence ID" value="XM_002552745.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DG11"/>
    <dbReference type="STRING" id="559295.C5DG11"/>
    <dbReference type="GeneID" id="8295011"/>
    <dbReference type="KEGG" id="lth:KLTH0D01540g"/>
    <dbReference type="eggNOG" id="KOG2588">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067895_0_0_1"/>
    <dbReference type="InParanoid" id="C5DG11"/>
    <dbReference type="OMA" id="FPENNAR"/>
    <dbReference type="OrthoDB" id="2133190at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11395">
        <property type="entry name" value="bHLHzip_SREBP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052099">
        <property type="entry name" value="Regulatory_TF_Diverse"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47336:SF3">
        <property type="entry name" value="SERINE-RICH PROTEIN TYE7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47336">
        <property type="entry name" value="TRANSCRIPTION FACTOR HMS1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BHLH" evidence="3">
        <location>
            <begin position="241"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="51"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="321"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="53"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR22353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="348" mass="35168" checksum="2A97E4203CEAA38B" modified="2009-07-28" version="1">MDGPGSSTMTMAAAPSAQGAGAGASSFDDWLFPEASRMGAFGEYGNDAEADAEAGGWAADAGAEADAEVDLDADADPEADADAGVGAAVGADPGAGVLAAGSLSSSSSSSSAGSPLDQFSSLFDSNAMLARGSDSDTLFTASNSASGSPTFVKREDAQMCGPAGLGASAGGLSAEGEAPGSLPLALDVPELSAFDGLDAAAAAADADVGGSAAANAAAAAAAAAAAGARKKRAPRKRLTAHQKQAHNKIEKRYRININTKIAKLQQIIPWVASEETAFEVGETSRGNSRGGESVATATPKLNKSMILEKAVDYILYLQNNERLYEMEVQRLKKEVERARTERDTAARS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5DG26</accession>
    <name>C5DG26_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">Pre-mRNA-processing protein 45</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0D01892g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in pre-mRNA splicing.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Associated with the spliceosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the SNW family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552806.1">
        <property type="nucleotide sequence ID" value="XM_002552760.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DG26"/>
    <dbReference type="FunCoup" id="C5DG26">
        <property type="interactions" value="1064"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DG26"/>
    <dbReference type="GeneID" id="8295026"/>
    <dbReference type="KEGG" id="lth:KLTH0D01892g"/>
    <dbReference type="eggNOG" id="KOG2441">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006601_3_1_1"/>
    <dbReference type="InParanoid" id="C5DG26"/>
    <dbReference type="OMA" id="EDQVYDN"/>
    <dbReference type="OrthoDB" id="666364at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017862">
        <property type="entry name" value="SKI-int_prot_SKIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004015">
        <property type="entry name" value="SKI-int_prot_SKIP_SNW-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12096">
        <property type="entry name" value="NUCLEAR PROTEIN SKIP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02731">
        <property type="entry name" value="SKIP_SNW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="SKI-interacting protein SKIP SNW" evidence="5">
        <location>
            <begin position="125"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="275"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="416"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="167"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="434"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU367140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR22368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="451" mass="50333" checksum="54E6630CC9225349" modified="2009-07-28" version="1">MSNSRPLSVLLPPPKHAINASAQHKSGLKELVGLLSEAGNPRETLYSVESFQENSVTLESFLPLRHKYPDLKIPQISQSDIQNTYERTKAVFDKILSQKLQPQGTGAVGTANKGSAKTIEYKLPDQNSEVRKLKIVDQQVDPLQPKLFKTKKVVAPSTDEPFAPVLHKSDDITPQSTKEERDKWNIPSAISSWKNPNGYTINLDRRIASDGRYSKESLGPHEISEGHMKLSEALDAAERKAREEVSLRADAKRMLAEQDVREKEEKLRMIALKAREDRKKNTEPYSGPRALDSATRQRQEDQRERLNKIRRELQRSKMSTAERLRALATGEGREVSEKVILGAAAASDTADIQYDSRLFTKAAKARGQSGGDQVYDNPLFVQEGINTMYRPNFSAAVDIQARDAADTLESKVQFELASGKRGREGPVEFSAAENTEKSEEGESSVKRHRGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGA5</accession>
    <name>C5DGA5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0D03718p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0D03718g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552885.1">
        <property type="nucleotide sequence ID" value="XM_002552839.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGA5"/>
    <dbReference type="STRING" id="559295.C5DGA5"/>
    <dbReference type="GeneID" id="8295109"/>
    <dbReference type="KEGG" id="lth:KLTH0D03718g"/>
    <dbReference type="eggNOG" id="ENOG502SAYY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_412228_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGA5"/>
    <dbReference type="OMA" id="FPACKYL"/>
    <dbReference type="OrthoDB" id="416217at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053157">
        <property type="entry name" value="Sterol_Uptake_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47784">
        <property type="entry name" value="STEROL UPTAKE CONTROL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47784:SF5">
        <property type="entry name" value="STEROL UPTAKE CONTROL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="2">
        <location>
            <begin position="51"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR22447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="666" mass="77304" checksum="570D9D1046C11BBE" modified="2009-07-28" version="1">MSRAPEDGFESMPSSRESEAGGQKRPNDCDQDHLSQKKRVTRRRHRNSRLGCDECKRRRIKCDETIPECRNCQNRQKREPGKRCSYLSMNEEELQSFKLKKQQNRLVEGTRVNKILEDHPDEIAYEIKMIAHESVELPVSVWTCVSGMESVRVPKALYLQVLLNANQDWDMSSRSFVVLALYSIMNSMSHKITLADQGQGDSAQEILLAEREILERIAVKYKKEFLTYAREIISMFLTSKAAVGNEYLGTRLLITNTVLQYIQLYSNAQYDSNHYAAMIDMCLEIYKNNDPEKVVRTVQFYWSSMPRNLFHLYFPAYSTDLLFELKAVLKEFEPIILAAGDERVQLHHKELGDFIHYVIGLLPLSTSVLPLPVDELYEMYRTWFLGIPSEAFCISRTMPGVQRVFYTFYHSIASYLNNLFPAGCYLMSRPFHGPTSLYPFSLNIVFEDLDHLLGPFAEFSVRLLSFMERREHILQNFFEVKDPLPPGLHKFRFQKGEVRNLKEKFISSLREELITWDNYPDLVPNTEHSEGKDMKYFVLTEREAAKSQIQRWRQAKNTYSGAEHFPTESNGLLIDQILEQMKSADPFLAMQRKKPLGSFGIEYSSDRGYQMGDRGLFQQDCDVSLFFEQSSPAFLSVLPSVEHVQDYKADRICVVSNTGSRKLYNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGC6</accession>
    <name>C5DGC6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Aspartate aminotransferase</fullName>
            <ecNumber evidence="7">2.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0D04224g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:21824"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="EC" id="2.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3 7">Homodimer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="7">In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.1" evidence="7"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552906.1">
        <property type="nucleotide sequence ID" value="XM_002552860.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGC6"/>
    <dbReference type="FunCoup" id="C5DGC6">
        <property type="interactions" value="900"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGC6"/>
    <dbReference type="GeneID" id="8295132"/>
    <dbReference type="KEGG" id="lth:KLTH0D04224g"/>
    <dbReference type="eggNOG" id="KOG1412">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032440_1_2_1"/>
    <dbReference type="InParanoid" id="C5DGC6"/>
    <dbReference type="OMA" id="GTWTHIT"/>
    <dbReference type="OrthoDB" id="6752799at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004069">
        <property type="term" value="F:L-aspartate:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006532">
        <property type="term" value="P:aspartate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000064">
        <property type="entry name" value="Aspartate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000001">
        <property type="entry name" value="Aspartate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000796">
        <property type="entry name" value="Asp_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006719">
        <property type="entry name" value="PRK09257.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879:SF55">
        <property type="entry name" value="GLUTAMATE OXALOACETATE TRANSAMINASE 1, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00799">
        <property type="entry name" value="TRANSAMINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="8">
        <location>
            <begin position="32"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR22468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="420" mass="45811" checksum="CE6E01DFB1A2EA4A" modified="2009-07-28" version="1">MSCTIFNNVEQLPPDALFGIKQRLSQDPRPVKVDLGIGAYRDDSGKPWVLPSVREAEEVIRQDPGYNHEYLGISGLTALIQGAAKVLFGEDSAALAEGRVVSTQSLSGTGALHVAARFISKFCGDKVVYLSDPTWANHNAIFETMGLKTATYPYWDAASKSLNLEGYLEAIKTAPKGSVFVLHACAHNPTGLDPDQSQWTSILDAIIEREHIVLFDSAYQGFASGDLDRDAFAVRLGLQKLADVSPVIVCQSFAKNVGMYGERAGCFHLVLPRQDASVDTSAIKSAVLSQLNKITRSELSNPPAYGAKIVATVLNTPRLTEQWHKDMVTMSSRIIKMRHALRDHLAKLNTPGNWDHIVNQTGMFSYTGLRPEMVKRLEEEHAVYLVSSGRASIAGLNSGNVEYVAKAINEVVTHFGGAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGC8</accession>
    <name>C5DGC8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Vacuolar-sorting protein SNF7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Vacuolar protein-sorting-associated protein 32</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0D04268g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF7 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552908.1">
        <property type="nucleotide sequence ID" value="XM_002552862.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGC8"/>
    <dbReference type="FunCoup" id="C5DGC8">
        <property type="interactions" value="734"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGC8"/>
    <dbReference type="GeneID" id="8295134"/>
    <dbReference type="KEGG" id="lth:KLTH0D04268g"/>
    <dbReference type="eggNOG" id="KOG1656">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071097_1_0_1"/>
    <dbReference type="InParanoid" id="C5DGC8"/>
    <dbReference type="OMA" id="MKQIHGG"/>
    <dbReference type="OrthoDB" id="5592979at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000815">
        <property type="term" value="C:ESCRT III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005771">
        <property type="term" value="C:multivesicular body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006900">
        <property type="term" value="P:vesicle budding from membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1060">
        <property type="entry name" value="ESAT-6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761:SF10">
        <property type="entry name" value="GH13992P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0967">Endosome</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="176"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="62"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="206"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="220"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00042586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR22470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="231" mass="25405" checksum="29081FC7445672CE" modified="2009-07-28" version="1">MLSYFFGGGSTSKNKDAPKKAILDLREHITLLGKKEAHLQTQVASQEQQARTLLGKNNKTGAKNALKRKKTYESQLVKIQAQMDSLEQQLYSIESANLNLETMKAMKQGASAMKNIHKGMDIDKVDETMDEIREQVELGEEISDAISRPLHAGAEVDEDEIDEELAALAQESVKQEVVAPPQGLPSVPLGKVSTPHAATLPAGQEQDAEAEEEEEDEDERALRELQAEMGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGD2</accession>
    <name>C5DGD2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">[RNA-polymerase]-subunit kinase</fullName>
            <ecNumber evidence="2">2.7.11.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0D04356g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>[DNA-directed RNA polymerase] + ATP = phospho-[DNA-directed RNA polymerase] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10216"/>
            <dbReference type="Rhea" id="RHEA-COMP:11321"/>
            <dbReference type="Rhea" id="RHEA-COMP:11322"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43176"/>
            <dbReference type="ChEBI" id="CHEBI:68546"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.23"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.23" evidence="2"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552912.1">
        <property type="nucleotide sequence ID" value="XM_002552866.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGD2"/>
    <dbReference type="FunCoup" id="C5DGD2">
        <property type="interactions" value="863"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGD2"/>
    <dbReference type="GeneID" id="8295138"/>
    <dbReference type="KEGG" id="lth:KLTH0D04356g"/>
    <dbReference type="eggNOG" id="KOG0600">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_181_1_1"/>
    <dbReference type="InParanoid" id="C5DGD2"/>
    <dbReference type="OMA" id="FPHCDES"/>
    <dbReference type="OrthoDB" id="204883at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008024">
        <property type="term" value="C:cyclin/CDK positive transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030332">
        <property type="term" value="F:cyclin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008353">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07840">
        <property type="entry name" value="STKc_CDK9_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000415">
        <property type="entry name" value="CMGC/CDK/CRK7 protein kinase, variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000588">
        <property type="entry name" value="CTD kinase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF546">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="139"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="23"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="447"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="89"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="104"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="454"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR22474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="469" mass="53744" checksum="01500097D9B493CE" modified="2009-07-28" version="1">MLKNWKLYFLVLRHRKTTRCIKTTPLLDRTGPRPTGPSDASRNGPHSAFPERSNGHQVSAAGSLGPERKRPRVGNSRYAGSRYQDAGFHSSSKQRLNVQNIPTGHRDVPKRQSRYDPGNAKPKAVPVTVVVRERDSSVYERIQQVGEGTYGKVYKARNTLTGQLVALKRLRLEGEREGFPITSIREIKLLQSFDHRNISTLSEIMVESQKTVYMIFEYADNDLSGLLMNEQIVLSSANCKHLFKSLLEGIHYLHSNGILHRDIKGSNILIDNKGQLKITDFGLARKMRDDSDYTNRVITLWYRPPELLMGSTNYGTAVDMWGCGCLLVELFQKTAIFQGTNEVEQLNAIFSIMGTPSVEQWPTLFEMPWFFMMIPQQNRKYEPRFDEKFGAVLPSPAAVSLAKGLLLYDEKRRLSASEALRHQYFREEPQPQPLDLSGFDGWHEFEAKRHRRKEREEQKRREKSESQGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGQ8</accession>
    <name>C5DGQ8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0D07304p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0D07304g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553038.1">
        <property type="nucleotide sequence ID" value="XM_002552992.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGQ8"/>
    <dbReference type="FunCoup" id="C5DGQ8">
        <property type="interactions" value="729"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGQ8"/>
    <dbReference type="MEROPS" id="C85.008"/>
    <dbReference type="GeneID" id="8295270"/>
    <dbReference type="KEGG" id="lth:KLTH0D07304g"/>
    <dbReference type="eggNOG" id="KOG2606">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034963_2_0_1"/>
    <dbReference type="InParanoid" id="C5DGQ8"/>
    <dbReference type="OMA" id="YELGAHY"/>
    <dbReference type="OrthoDB" id="415023at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22762">
        <property type="entry name" value="OTU_fungi_OTU2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049771">
        <property type="entry name" value="OTU2-like_OTU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003323">
        <property type="entry name" value="OTU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050704">
        <property type="entry name" value="Peptidase_C85-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12419:SF10">
        <property type="entry name" value="DEUBIQUITINASE OTUD6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12419">
        <property type="entry name" value="OTU DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02338">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50802">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OTU" evidence="2">
        <location>
            <begin position="166"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR22600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="319" mass="36456" checksum="426036FF6F08DF43" modified="2009-07-28" version="1">MDTEEAILARHRAEKKDLQNKIAGMKKQATKSKRKQVNSRCEELEHDLLQRQEKELQTFRNEGSSDKQASNEPLQESSDDEITPEKLLAQLELGVSAQNSPSVDADKQQTAPQPKKRRNRQKERLAKRDAEMARIREAAAQEAAVQPNLKQIEQDALDQLCDVKGVKQFDIKPDGHCLFASILDQVWLRHPTDAQYFIPSGYDGPHLPRDLDVRALRSLSSNYIRQYRDDFVPYLFDEATMSIKDVDDYTKTMEETAQWGGEVELLALAKVLKCCISVMMSGRATHMINEQEVANPELKLVYYKHSFALGEHYNSLHDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DH37</accession>
    <name>C5DH37_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="15">KLTH0E01078p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0E01078g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the 60S subunit of the ribosome.</text>
    </comment>
    <comment type="function">
        <text evidence="12">Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. eL40 is essential for translation of a subset of cellular transcripts, including stress response transcripts, such as DDR2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Component of the large ribosomal subunit (LSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes).</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Part of the 60S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">In the C-terminal section; belongs to the eukaryotic ribosomal protein eL40 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the ubiquitin family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553535.1">
        <property type="nucleotide sequence ID" value="XM_002553489.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DH37"/>
    <dbReference type="FunCoup" id="C5DH37">
        <property type="interactions" value="1048"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DH37"/>
    <dbReference type="GeneID" id="8291673"/>
    <dbReference type="KEGG" id="lth:KLTH0E01078g"/>
    <dbReference type="eggNOG" id="KOG0003">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010412_3_4_1"/>
    <dbReference type="InParanoid" id="C5DH37"/>
    <dbReference type="OMA" id="CGRCSQL"/>
    <dbReference type="OrthoDB" id="428577at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01803">
        <property type="entry name" value="Ubl_ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000014">
        <property type="entry name" value="Ubiquitin-60S ribosomal L40 fusion"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1060.50:FF:000001">
        <property type="entry name" value="ubiquitin-60S ribosomal protein L40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1060.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001975">
        <property type="entry name" value="Ribosomal_eL40_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038587">
        <property type="entry name" value="Ribosomal_eL40_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019954">
        <property type="entry name" value="Ubiquitin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019956">
        <property type="entry name" value="Ubiquitin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050158">
        <property type="entry name" value="Ubiquitin_ubiquitin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10666">
        <property type="entry name" value="UBIQUITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01020">
        <property type="entry name" value="Ribosomal_L40e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00348">
        <property type="entry name" value="UBIQUITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01377">
        <property type="entry name" value="Ribosomal_L40e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00299">
        <property type="entry name" value="UBIQUITIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="8" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="14">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00035124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00064933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR23098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="128" mass="14524" checksum="E51F807A4AB47F83" modified="2009-07-28" version="1">MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGIIEPSLKALASKFNCEKSVCRKCYARLPPKATNCRKRKCGHTNQLRPKKKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>C5DH73</accession>
    <name>C5DH73_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Superoxide dismutase [Cu-Zn]</fullName>
            <ecNumber evidence="8">1.15.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0E01892g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Destroys radicals which are normally produced within the cells and which are toxic to biological systems.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>2 superoxide + 2 H(+) = H2O2 + O2</text>
            <dbReference type="Rhea" id="RHEA:20696"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:18421"/>
            <dbReference type="EC" id="1.15.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="8">Binds 1 copper ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the Cu-Zn superoxide dismutase family.</text>
    </comment>
    <dbReference type="EC" id="1.15.1.1" evidence="8"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553571.1">
        <property type="nucleotide sequence ID" value="XM_002553525.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DH73"/>
    <dbReference type="FunCoup" id="C5DH73">
        <property type="interactions" value="846"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DH73"/>
    <dbReference type="GeneID" id="8291710"/>
    <dbReference type="KEGG" id="lth:KLTH0E01892g"/>
    <dbReference type="eggNOG" id="KOG0441">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056632_4_1_1"/>
    <dbReference type="InParanoid" id="C5DH73"/>
    <dbReference type="OMA" id="AQRGFHI"/>
    <dbReference type="OrthoDB" id="2015551at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00305">
        <property type="entry name" value="Cu-Zn_Superoxide_Dismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.200:FF:000001">
        <property type="entry name" value="Superoxide dismutase [Cu-Zn]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.200">
        <property type="entry name" value="Superoxide dismutase, copper/zinc binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036423">
        <property type="entry name" value="SOD-like_Cu/Zn_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024134">
        <property type="entry name" value="SOD_Cu/Zn_/chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018152">
        <property type="entry name" value="SOD_Cu/Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001424">
        <property type="entry name" value="SOD_Cu_Zn_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10003">
        <property type="entry name" value="SUPEROXIDE DISMUTASE CU-ZN -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00080">
        <property type="entry name" value="Sod_Cu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00068">
        <property type="entry name" value="CUZNDISMTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49329">
        <property type="entry name" value="Cu,Zn superoxide dismutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00087">
        <property type="entry name" value="SOD_CU_ZN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00332">
        <property type="entry name" value="SOD_CU_ZN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="6 8" id="KW-0186">Copper</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Superoxide dismutase copper/zinc binding" evidence="9">
        <location>
            <begin position="14"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR23134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="154" mass="15745" checksum="144DDE4F45B58D35" modified="2009-07-28" version="1">MVKAVAVLRGDAGVSGTVHLEQKAENEPTTVSYEIAGFGSSGDHGFHIHEFGDNTNGCTSAGPHFNPFKKTHGSPSDEVRHVGDLGNIAANDKGVCKGVLTDSLVKLIGPTSVLGRTVVVHSGQDDLGKGGNEESLKTGNAGTRPACGVIGISN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>C5DH86</accession>
    <name>C5DH86_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0E02178p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0E02178g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="5">Part of a larger complex that includes HSP70, HSP90, and immunophilins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553584.1">
        <property type="nucleotide sequence ID" value="XM_002553538.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DH86"/>
    <dbReference type="FunCoup" id="C5DH86">
        <property type="interactions" value="1084"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DH86"/>
    <dbReference type="GeneID" id="8291723"/>
    <dbReference type="KEGG" id="lth:KLTH0E02178g"/>
    <dbReference type="eggNOG" id="KOG0548">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000134_46_5_1"/>
    <dbReference type="InParanoid" id="C5DH86"/>
    <dbReference type="OMA" id="MYSAREN"/>
    <dbReference type="OrthoDB" id="2423701at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042030">
        <property type="term" value="F:ATPase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051879">
        <property type="term" value="F:Hsp90 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.100:FF:000004">
        <property type="entry name" value="Putative stress-induced-phosphoprotein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000010">
        <property type="entry name" value="Stress-induced phosphoprotein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000020">
        <property type="entry name" value="Stress-induced phosphoprotein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.100:FF:000002">
        <property type="entry name" value="Stress-induced-phosphoprotein 1 (Hsp70/Hsp90-organizing)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000027">
        <property type="entry name" value="stress-induced-phosphoprotein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041243">
        <property type="entry name" value="STI1/HOP_DP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006636">
        <property type="entry name" value="STI1_HS-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058209">
        <property type="entry name" value="TPR_BSK1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22904:SF523">
        <property type="entry name" value="STRESS-INDUCED-PHOSPHOPROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22904">
        <property type="entry name" value="TPR REPEAT CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17830">
        <property type="entry name" value="STI1-HOP_DP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25575">
        <property type="entry name" value="TPR_BSK1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00727">
        <property type="entry name" value="STI1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="6">
        <location>
            <begin position="3"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="6">
        <location>
            <begin position="72"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="STI1" evidence="8">
        <location>
            <begin position="135"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="6">
        <location>
            <begin position="267"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="6">
        <location>
            <begin position="341"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="6">
        <location>
            <begin position="401"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="STI1" evidence="8">
        <location>
            <begin position="542"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="195"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="209"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="261"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00064323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR23147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="593" mass="65914" checksum="9DBA550BC32E597D" modified="2009-07-28" version="1">MSAEEFKKQGNAAFVAKDYEKASELFTKAIEASEAPNHVLYSNRSACFTSLKKFNEALNDANECVKINPAWSKGYNRVGAAYFGLGKLDEAEQGYKKALEIDTSNKAAQDGLEQVQKAQKSQQQPDLGFGQMFSDPNMIEKLKNNPKTAELMKDPQLVSKVLQFQSNPQMMGPEILQDPRMMTIMAAMLGVDLSIGGDSNGQEPAAPSKEAPATKETASEAESAKPAPEAKAAPSASSAEASSSATADAGPMEVDEPQPEQDSKKNADAEKAEGNKFYKARKFDEAIEHYNKAWDLHKDVTYLNNRSAAEYEKGDYEQAIKTLTEAVEQGREMRADYKLISKSFARIGNAYLKLEDFKKAIEFYQKSLTEHRTPDILTKLRNCEKEQKVREAQAYIDPEKAEEARLQGKEYFTKGDWPNAVKAYTEMTKRAPEDARGYSNRAAALAKLMSFPEAIKDCDMAISKDPNFIRAYIRKATAQIAVREFASAIETLDAARAKDTELNNGANVREIDQLYIKASQQRFQPQDPNETPEQAYARALKDPEVASIMQDPVMQSILSQAQQNPAALQEHMKNPEVFNKIQTLIAAGIIRTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>C5DH87</accession>
    <name>C5DH87_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0E02200p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0E02200g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the ClpA/ClpB family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553585.1">
        <property type="nucleotide sequence ID" value="XM_002553539.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DH87"/>
    <dbReference type="SMR" id="C5DH87"/>
    <dbReference type="FunCoup" id="C5DH87">
        <property type="interactions" value="236"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DH87"/>
    <dbReference type="GeneID" id="8291724"/>
    <dbReference type="KEGG" id="lth:KLTH0E02200g"/>
    <dbReference type="eggNOG" id="KOG1051">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005070_4_0_1"/>
    <dbReference type="InParanoid" id="C5DH87"/>
    <dbReference type="OMA" id="VSKMMQG"/>
    <dbReference type="OrthoDB" id="47330at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043335">
        <property type="term" value="P:protein unfolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19499">
        <property type="entry name" value="RecA-like_ClpB_Hsp104-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000017">
        <property type="entry name" value="ATP-dependent chaperone ClpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000120">
        <property type="entry name" value="ATP-dependent chaperone ClpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000025">
        <property type="entry name" value="ATP-dependent Clp protease subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000010">
        <property type="entry name" value="Chaperone clpB 1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019489">
        <property type="entry name" value="Clp_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001270">
        <property type="entry name" value="ClpA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018368">
        <property type="entry name" value="ClpA/B_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028299">
        <property type="entry name" value="ClpA/B_CS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041546">
        <property type="entry name" value="ClpA/ClpB_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050130">
        <property type="entry name" value="ClpA_ClpB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11638">
        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11638:SF176">
        <property type="entry name" value="HEAT SHOCK PROTEIN 78, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07724">
        <property type="entry name" value="AAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17871">
        <property type="entry name" value="AAA_lid_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10431">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00300">
        <property type="entry name" value="CLPPROTEASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01086">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00870">
        <property type="entry name" value="CLPAB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00871">
        <property type="entry name" value="CLPAB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="8">
        <location>
            <begin position="106"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="8">
        <location>
            <begin position="502"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="domain" description="Clp ATPase C-terminal" evidence="9">
        <location>
            <begin position="675"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="318"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR23148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="780" mass="87496" checksum="C98A454973CC4ADF" modified="2009-07-28" version="1">MLRLARTSALRASLARTATAASTRAAMASPARRLSALRVPATRFTRPCNQYRTYVSLNMNNQEPQKPALEQFGTNLTQLAQEGKLDPVIGRDEEIARAIQILSRRTKNNPVLIGRAGVGKTSLIDGLAQRIVSGQVPDSLKNKKLVTLDLGSLVAGAKYRGEFEERLKNVLEEIDKSNGEVIVFIDEVHMLLGLGKTDGSMDASNILKPRLAKGLRCISATTIDEFKIIEKDPALSRRFQPIMLNEPNVADTISILRGLKERYEVHHGVRITDSALVSAATLSNRYINDRFLPDKAIDLVDEACAVLRLQHESKPDAIQSLDRAIMRIQIELESLKKETDPVSLERRTALEDELELKRKEHARLTEIWEKEKAEIESIKTAKADLEQARIDLEITQREGNFAKASELRYAKIPELERRVARNAQKDAKENLLHDSVSSDDISNVIAKMTGIPMETVLKGDKDRLLYMEDSLRKRVVGQDEAIDAVSDAVRLQRAGLTSEKRPIASFMFLGPTGTGKTELTKALAEFLFDNESNVIRFDMSEFQEKHTVSRLIGAPPGYVMSESGGQLTEAVRRKPYAVVLFDEFEKAHPDVCKVLLQVLDEGKLTDSQGHQVDFRNTIIVMTSNIGQDILLSDTELGNDGKVSKATQERVIEAMKKSYPPEFINRIDDVLVFNRLSKDVLRSIVDIRIEEIQERIADKRMKLNISDAAKEWLTDNGYDQLYGARPLNRLIHKRILNSMAMYLLRGQIRPEETVNVDVKDGKLVVEPNHEEGEVVPAKDEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHD9</accession>
    <name>C5DHD9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0E03586p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0E03586g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the STE12 transcription factor family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553637.1">
        <property type="nucleotide sequence ID" value="XM_002553591.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHD9"/>
    <dbReference type="FunCoup" id="C5DHD9">
        <property type="interactions" value="2697"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHD9"/>
    <dbReference type="GeneID" id="8291786"/>
    <dbReference type="KEGG" id="lth:KLTH0E03586g"/>
    <dbReference type="eggNOG" id="ENOG502QTVR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019798_0_0_1"/>
    <dbReference type="InParanoid" id="C5DHD9"/>
    <dbReference type="OMA" id="YPINPYY"/>
    <dbReference type="OrthoDB" id="1095242at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990526">
        <property type="term" value="C:Ste12p-Dig1p-Dig2p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990527">
        <property type="term" value="C:Tec1p-Ste12p-Dig1p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000220">
        <property type="term" value="P:regulation of pseudohyphal growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003120">
        <property type="entry name" value="Ste12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052127">
        <property type="entry name" value="STE12_transcription_factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47427">
        <property type="entry name" value="PROTEIN STE12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47427:SF1">
        <property type="entry name" value="PROTEIN STE12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02200">
        <property type="entry name" value="STE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00424">
        <property type="entry name" value="STE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="215"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="602"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="215"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="627"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="673"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="684"/>
            <end position="701"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR23200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="701" mass="77779" checksum="3CE442BA0DE56D56" modified="2009-07-28" version="1">MLRGQPNAQEIVKTKAEGDSEESTPEQVEESLRLIEDLKFFLATAPANWQENQIIRRYYLNNDQGFVSCVFWNNLYYITGTDIVKCCVYRMQKFGREVLDRKKFEEGIFSDLRNLKCGIDATLEMPKSEFLSFLFKNMCLKTQKKQKVFFWFSVPHDKFFAYALERDMKRQSAGQLSTTRAVSEPALSFTYDDKSGLSLYDQLLQHMESQRIKVNASSSVDSTTANPQLTSYSPAESDDPDSDAVIKTEFVGSSQESLAQISKYAPQELVVDPIEEGADEQPFELDSGAQAPFLLKDASQEEDDFPLDYFPVEVEYPNQQQLQPGGGQVYYDNDFEVEYVPLSAVPPASAGFYDNSHFTEETAFAAPAVTSAARLAFQIPPPPMSASRSHFMTNGEYYASYVKDQEDRGCNRASDADVALDRSDAEEVVANGGDGTMNSKQGGMLHKPNIDFRAQNGGYPVGSFYQGKHAYPVELQQGGADPHLTRSYAMEDYLCDNSALYEGFPGGAYLPPAANPFTPSFLMTPVAPTSPYIGVTQYGAKQVVPASAKNYPINPYYQSSPGFQRWGNVHGYVRGFPSIQATSAPPGAPLQMHNPQSASKLYFNKPGMIHKPPSRSQNQRHRARKTAFSSRPPMNSSHTSNRSTSDGGNSVAFLVPSSESSMVLPDGSKKKLSTSASANEPSSTDDKEIIKKHEDDNQFHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHI4</accession>
    <name>C5DHI4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0E04576p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0E04576g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553682.1">
        <property type="nucleotide sequence ID" value="XM_002553636.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHI4"/>
    <dbReference type="STRING" id="559295.C5DHI4"/>
    <dbReference type="GeneID" id="8291831"/>
    <dbReference type="KEGG" id="lth:KLTH0E04576g"/>
    <dbReference type="eggNOG" id="ENOG502SE22">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_156033_1_0_1"/>
    <dbReference type="InParanoid" id="C5DHI4"/>
    <dbReference type="OMA" id="QGFLWTG"/>
    <dbReference type="OrthoDB" id="2430343at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140580">
        <property type="term" value="F:mitochondrion autophagosome adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013898">
        <property type="entry name" value="Atg43"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38699">
        <property type="entry name" value="CHROMOSOME 1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38699:SF1">
        <property type="entry name" value="MITOPHAGY RECEPTOR ATG43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR23245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="115" mass="12837" checksum="35E8A0356FA3CE49" modified="2009-07-28" version="1">MNGLKPMTGAQKVAVPDLRFEQTFQRALRREASKNQKATSQGHSTTDADATISASVICKVVLRDVLLMPFLQGILWTSALIALKPWLHMVRIRGQLVGHRIYEMVLGKGLVRKRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHP0</accession>
    <name>C5DHP0_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Pre-mRNA-processing factor 19</fullName>
            <ecNumber evidence="14">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">KLTH0E05896g</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Ubiquitin-protein ligase which is mainly involved pre-mRNA splicing and DNA repair. Required for pre-mRNA splicing as component of the spliceosome.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the WD repeat PRP19 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="14"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553738.1">
        <property type="nucleotide sequence ID" value="XM_002553692.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHP0"/>
    <dbReference type="FunCoup" id="C5DHP0">
        <property type="interactions" value="486"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHP0"/>
    <dbReference type="GeneID" id="8291891"/>
    <dbReference type="KEGG" id="lth:KLTH0E05896g"/>
    <dbReference type="eggNOG" id="KOG0289">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023894_0_0_1"/>
    <dbReference type="InParanoid" id="C5DHP0"/>
    <dbReference type="OMA" id="MIAYSNE"/>
    <dbReference type="OrthoDB" id="687049at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071006">
        <property type="term" value="C:U2-type catalytic step 1 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070534">
        <property type="term" value="P:protein K63-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16656">
        <property type="entry name" value="RING-Ubox_PRP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000027">
        <property type="entry name" value="Pre-mRNA-processing factor 19, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038959">
        <property type="entry name" value="Prp19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013915">
        <property type="entry name" value="Prp19_cc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055340">
        <property type="entry name" value="RING-Ubox_PRP19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43995">
        <property type="entry name" value="PRE-MRNA-PROCESSING FACTOR 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43995:SF1">
        <property type="entry name" value="PRE-MRNA-PROCESSING FACTOR 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08606">
        <property type="entry name" value="Prp19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9 14" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12 14" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5 14" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="11 14" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="13 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 14" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="6 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="10 14" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="U-box" evidence="16">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="116"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU367101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAR23301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="517" mass="56990" checksum="0BB585B08E7354EF" modified="2009-07-28" version="1">MFCAISGKPPKTASFSPSSKCVFEKSLIEAYVAENGIDPISKEPLQIDQLVEIAKAPEQYAMSNSVNSPSLNSNYSIPNLLSALQDEWDAVMLENFQLRQQIEASKKELSTALYRCDAAMNVAARATMEADKLKQELNILTTNLAPAKAQDTQLPSAEQGLVSELPRDLVAELGARSRNFAAESRKIKFSAPSCSEMTRASDVEPVNIPKGEARIAAAFIDGNIANGKLISFYNADGDCYVCSAASSWKGYQNVTFEGNALISYAAPLNAAEMIVGTQDGRHGIYDLNTEKISTAFHSNDESPVVFALGLQEVYGGSYIAINARGRILYVVPEERHVFDAEVDSFPTEFAHIHKDGLLIAQANSAQLIVRDLTNLHEKPIHYLHDVEKEGEITSARFASNGYWLIVSAQKALKVFDLRKTPNTLAADPILFENETLKAWDIEPSMKILFTVTEDANGTNLIRRYRFTKASKSWTLESSINCEDLLEVKEVHGLSYLWDQASGFLKVQNGNELITVRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHP5</accession>
    <name>C5DHP5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0E06006p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0E06006g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553743.1">
        <property type="nucleotide sequence ID" value="XM_002553697.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHP5"/>
    <dbReference type="FunCoup" id="C5DHP5">
        <property type="interactions" value="1096"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHP5"/>
    <dbReference type="GeneID" id="8291896"/>
    <dbReference type="KEGG" id="lth:KLTH0E06006g"/>
    <dbReference type="eggNOG" id="KOG0733">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_8_2_1"/>
    <dbReference type="InParanoid" id="C5DHP5"/>
    <dbReference type="OMA" id="GLWSTHR"/>
    <dbReference type="OrthoDB" id="27435at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990275">
        <property type="term" value="F:preribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19518">
        <property type="entry name" value="RecA-like_NVL_r1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19530">
        <property type="entry name" value="RecA-like_NVL_r2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000018">
        <property type="entry name" value="Cell division control 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000110">
        <property type="entry name" value="Ribosome biogenesis ATPase RIX7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000156">
        <property type="entry name" value="Ribosome biogenesis ATPase RIX7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000365">
        <property type="entry name" value="Ribosome biogenesis ATPase RIX7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015415">
        <property type="entry name" value="Spast_Vps4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF171">
        <property type="entry name" value="NUCLEAR VALOSIN-CONTAINING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09336">
        <property type="entry name" value="Vps4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="9">
        <location>
            <begin position="235"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="9">
        <location>
            <begin position="555"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="36"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="160"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="36"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="48"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="160"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="179"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="196"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR23306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="819" mass="90406" checksum="81EB56B9EF873A7E" modified="2009-07-28" version="1">MVKARKSKAPLSQGFDNKISDLIYLMLDEKSLEKRRANAAEKARREEEELADEEETAEDDFSDIFLARDLSFGEVLAFVQSKDLSLQRVKKVVLEKTVDKVLKQAIEEEEEQFAAYFANETSKDDQMNLDSESLSQQNLMVTPTSNDMNKSITSIWATTKSEENKEDGDKKLSSKKRAKETGSKPKRQKTQEDRSPPSSNLSSLGGMDDVVAQLMELIGLPILHPEIFASTGVEPPRGVLLHGPPGCGKTSIANALAGELQVPFISISAPSVVSGMSGESEKKIRDLFEEAKSLAPCLVFFDEIDAITPKRDGGAQREMERRIVAQLLTSMDELSFEKTGGKPVIIIGATNRPDSLDAALRRAGRFDREICLNVPNEISRLHILKKMSSTLKIDGSIDFLKLAKLTPGFVGADLKALSTAAGTCAIKRIFQSYASLAPTSMEVDDETLTSDGSQEPKNTVNMIDPLPLSTVQKFIQSFPEPLTEEQLASLSIRYEDFLKALPTIQPTAKREGFATVPDVSWANIGALNRVRIELNMAIVQPIKRPELYEKVGISAPAGVLLWGPPGCGKTLLAKAVANESRANFISIKGPELLNKYVGESERAIRQVFSRARASVPCVIFFDELDALVPRRDASLSESSSRVVNTLLTELDGLNDRRGIFVIGATNRPDMIDAAMLRPGRLDKTLFIELPNFEEKLDIIKTLARTNGTPLSSDVDFEAIIRDESCRNFSGADLAALIRESSVLALKRSFFRTEEVHSITENNLEKEFGELNTSSEDVIVTMQDFSNALKKVKPSVSDKDRQKYERLNKKMGWVDEADGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHQ8</accession>
    <name>C5DHQ8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="8">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0E06314g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the PPP phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="8"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553756.1">
        <property type="nucleotide sequence ID" value="XM_002553710.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHQ8"/>
    <dbReference type="FunCoup" id="C5DHQ8">
        <property type="interactions" value="1186"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHQ8"/>
    <dbReference type="GeneID" id="8291910"/>
    <dbReference type="KEGG" id="lth:KLTH0E06314g"/>
    <dbReference type="eggNOG" id="KOG0374">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004962_0_0_1"/>
    <dbReference type="InParanoid" id="C5DHQ8"/>
    <dbReference type="OMA" id="EEHEIRY"/>
    <dbReference type="OrthoDB" id="1930084at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000164">
        <property type="term" value="C:protein phosphatase type 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007346">
        <property type="term" value="P:regulation of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07414">
        <property type="entry name" value="MPP_PP1_PPKL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000004">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050341">
        <property type="entry name" value="PP1_catalytic_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031675">
        <property type="entry name" value="STPPase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668">
        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668:SF300">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16891">
        <property type="entry name" value="STPPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="9">
        <location>
            <begin position="120"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR23319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="311" mass="35841" checksum="BA1ED2801D3E0E60" modified="2009-07-28" version="1">METPAVDLDNIIDRLLEVRGSKPGQQVDLEEHEIRFLCSKARSIFIKQPILLELEAPIKICGDIHGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQSLETICLLLAYKIKYPENFFILRGNHECASINRIYGFYDECKRRYNIKLWKTFTDCFNCLPIAAIIDEKIFCMHGGLSPDLNTMEQIRRVMRPTDIPDVGLLCDLLWSDPDKDIVGWSENDRGVSFTFGPDVVSRFLQKQDMELICRAHQVVEDGYEFFSKRQLVTLFSAPNYCGEFDNAGAMMSVDESLLCSFQILKPAQKTLPRPQGKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHS3</accession>
    <name>C5DHS3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Meiotic nuclear division protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0E06710g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Required for proper homologous chromosome pairing and efficient cross-over and intragenic recombination during meiosis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the MND1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553771.1">
        <property type="nucleotide sequence ID" value="XM_002553725.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHS3"/>
    <dbReference type="FunCoup" id="C5DHS3">
        <property type="interactions" value="206"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHS3"/>
    <dbReference type="GeneID" id="8291926"/>
    <dbReference type="KEGG" id="lth:KLTH0E06710g"/>
    <dbReference type="eggNOG" id="KOG3433">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080628_2_0_1"/>
    <dbReference type="InParanoid" id="C5DHS3"/>
    <dbReference type="OMA" id="VCYWAFP"/>
    <dbReference type="OrthoDB" id="9978204at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005647">
        <property type="entry name" value="Mnd1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040453">
        <property type="entry name" value="Mnd1_HTH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03962">
        <property type="entry name" value="Mnd1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF026991">
        <property type="entry name" value="Mnd1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mnd1 HTH" evidence="7">
        <location>
            <begin position="21"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="86"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR026991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR23334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="229" mass="26144" checksum="9278A0DEE26C854A" modified="2009-07-28" version="1">MPSVPPPKRTTISLAQKKSSILEFFQCEHSVYNIKELEKLIPKKCSGVASMLVKDLIQQMIDEDGIISVEKCGNTNIYWCFRNQIITKMSREIDMLNQRIEAAKLEISQTKMALDVSTKSSRKESFEVDGAVFSRAEKLEELESLDKAVKHSRSVYDQKSEHTWDQAKINAKTEALSAQVAKLDTVTDNIELIVSFLCRRFLMDKRSLQSELGIPEEFLEFTNMPQTSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHY9</accession>
    <name>C5DHY9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0E08272p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0E08272g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553837.1">
        <property type="nucleotide sequence ID" value="XM_002553791.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHY9"/>
    <dbReference type="FunCoup" id="C5DHY9">
        <property type="interactions" value="37"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHY9"/>
    <dbReference type="GeneID" id="8291997"/>
    <dbReference type="KEGG" id="lth:KLTH0E08272g"/>
    <dbReference type="eggNOG" id="ENOG502QVPM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005481_0_0_1"/>
    <dbReference type="InParanoid" id="C5DHY9"/>
    <dbReference type="OMA" id="MSSMIQR"/>
    <dbReference type="OrthoDB" id="68020at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000127">
        <property type="term" value="C:transcription factor TFIIIC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042791">
        <property type="term" value="P:5S class rRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006384">
        <property type="term" value="P:transcription initiation at RNA polymerase III promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16169">
        <property type="entry name" value="Tau138_eWH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046488">
        <property type="entry name" value="Sfc3/Tfc3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044210">
        <property type="entry name" value="Tfc3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035625">
        <property type="entry name" value="Tfc3-like_eWH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007309">
        <property type="entry name" value="TFIIIC_Bblock-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049543">
        <property type="entry name" value="WHD_TFC3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15180">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR 3C POLYPEPTIDE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15180:SF1">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR 3C POLYPEPTIDE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04182">
        <property type="entry name" value="B-block_TFIIIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20222">
        <property type="entry name" value="DUF6581"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21552">
        <property type="entry name" value="WHD_TFC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="B-block binding subunit of TFIIIC" evidence="7">
        <location>
            <begin position="107"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription factor tau 138 kDa subunit extended winged helix" evidence="9">
        <location>
            <begin position="555"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription factor tau subunit sfc3/Tfc3 C-terminal" evidence="8">
        <location>
            <begin position="742"/>
            <end position="1115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="267"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="685"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="270"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="690"/>
            <end position="706"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF21552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR23400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1158" mass="131239" checksum="D1A064C4C4D92A27" modified="2009-07-28" version="1">MALLAPDELVQRLCEEIAYNGGKITLGGFWDLVTELTNKPDERMKLFILRCFDSHPETAITKEGEVLEVSNYNDFYEDVQGVKCTISEDRLYMFLTGYRKKECLVGGLAFDLLVEIAKTKEKGINTMDLAQVTSQDPRSITGRIKKLGNLVSGVQTIYKGHVVKNLKFFRFVDGKEPSKSYTSMRDSLPKIVDVVKNSKNGVRQLIDLKRELKFDKDKRLSKSFIAAISSLDEMGYLKKVLVVSPSSPTVKIRCVKFLRDYVPEDRSANDFEDETDGGEDTDGKPTAESVMGEEDDENEIYGNENATQALQERNLVVEDEMKPERRTFLLNRFFPFQNQTFALVDNHGKTGLSSMQAVNLISGSDYKRSFTKCSEYYIETVGKDDDVGNGFGLVRAYDFEGKKKFYRLFTKSHFQNMVSAQRPESEGVFRPLKGQHATLQELSLKNFSPLSNTIRFIHTEGSDRFFWNGDLKVPANQNAAPRGRKRKQLKTEVNDNGTVKKVKQEIQGALEGPLDEGSRPGATSSLEKAGVTVSEGGEAEKQKAILNIGGFSAGSLKSLQRQRAILEAVKRNGGVTFFRDQFFDDVTKLMESKTTLDKKTIRGDVELLRATQKLVLKVDQSSGRRLMCLPNVSSEEISKFLVNEKDSKKSYFRDVIQNTDIYFFDQTQRDRFHRGKKSAERIKNFEHRSNSGRRTKASRPSKKPVKKLNAGLDDSSTNRQSLVENNMAQYKSGDSVHSPRYNVGTKAGVRALIMSAVITKSLRGQIMWDLITKLFPNNSISNLKKQWTARRIRMGHGGWKGLLEKWRKVIVDAMKDERVTMEDVEQLELPKLVELWENSDQERSKQSITLFKSHEENLRNLTIVKEPSSSACANNPDQSSMVQRETGLLRKCYTYVARDKELSFPVDDELRAIIRSILIDKSARDLEGIKLLERFSRDEVDKTILEMAKEKQVLFIGQSKLQLSDSVLDFLNKKGDDAFLKSAVNYRAQLFIFLNDQKGVLLNEEIKNHAALVLIDLMNSNKIGIAEVPLLPQEIQMHYSTRKYEVGALTPPLLITKTTVELPFKGHGPIPVGEPHSRIWVDSEGCIRKEIWRKVVALVLQEICFNPGITLRRLAGKNSQLLSMSEISEVITWCMKEKLVSKLAFEGLNIEGSWYSAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DI11</accession>
    <name>C5DI11_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">cysteine synthase</fullName>
            <ecNumber evidence="4">2.5.1.47</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Cysteine synthase-like protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0E08800g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate</text>
            <dbReference type="Rhea" id="RHEA:14829"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:58340"/>
            <dbReference type="EC" id="2.5.1.47"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion outer membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cysteine synthase/cystathionine beta-synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.47" evidence="4"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553859.1">
        <property type="nucleotide sequence ID" value="XM_002553813.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DI11"/>
    <dbReference type="FunCoup" id="C5DI11">
        <property type="interactions" value="256"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DI11"/>
    <dbReference type="GeneID" id="8292020"/>
    <dbReference type="KEGG" id="lth:KLTH0E08800g"/>
    <dbReference type="eggNOG" id="KOG1481">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021018_1_0_1"/>
    <dbReference type="InParanoid" id="C5DI11"/>
    <dbReference type="OMA" id="WMADYGF"/>
    <dbReference type="OrthoDB" id="10259545at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004124">
        <property type="term" value="F:cysteine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01561">
        <property type="entry name" value="CBS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000096">
        <property type="entry name" value="Related to cysteine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050214">
        <property type="entry name" value="Cys_Synth/Cystath_Beta-Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001216">
        <property type="entry name" value="P-phosphate_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10314">
        <property type="entry name" value="CYSTATHIONINE BETA-SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00901">
        <property type="entry name" value="CYS_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="13">
        <location>
            <begin position="45"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00078545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR23422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="391" mass="41748" checksum="F2A4A6AB2AF7BFF2" modified="2009-07-28" version="1">MDTRGVSWSNVAAVSTAAVAVAALYRAATASKTLTELAPPRRGVEDLIGNTPVIKIKSLSKATGCEVYAKMELCNPGGSAKDRVALGIVRAAERQGLLRPGFPDVVFEGTSGSTGISIATICNALGYRAHISLPDDTSAEKLALLESLGAQINKVRPAGIVDPEQYVNAAQRACNQLNQSGDGSHRGVFADQFENDANWRVHYQGTGPEIYRQLDGRIDAFIAGCGTGGTIAGVARFLKEKLGGRLRVVLADPQGSGFYNRVKFGVMYDRVEQEGTRRRHQVDTIVEGIGLNRITHNFSQGEPYIDDAERVTDGQAVRMAKFLSVNDGLFIGSSTAINAVAAVKVAESLPPGSRIVIIACDSGSRHLSKFWKEALCVDNETTLREVLAGSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>C5DI80</accession>
    <name>C5DI80_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0E10406p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0E10406g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553928.1">
        <property type="nucleotide sequence ID" value="XM_002553882.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DI80"/>
    <dbReference type="GeneID" id="8292093"/>
    <dbReference type="KEGG" id="lth:KLTH0E10406g"/>
    <dbReference type="eggNOG" id="KOG0254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_30_3_1"/>
    <dbReference type="InParanoid" id="C5DI80"/>
    <dbReference type="OMA" id="WATYLFY"/>
    <dbReference type="OrthoDB" id="648285at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000119">
        <property type="entry name" value="MFS monosaccharide transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF73">
        <property type="entry name" value="METABOLITE TRANSPORT PROTEIN YDL199C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="120"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="218"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="282"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="373"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="408"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="439"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="467"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="531"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="125"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="620"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="706"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="636"/>
            <end position="646"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR23491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="733" mass="79335" checksum="0CB9721D55706DD9" modified="2009-07-28" version="1">MAQRERSVSPKSRGKSPPEIHAANASAQSFTEQHRGASLTSLPRKKPEMYHYDVGGGAQAAAQAGAATAGAGMLYQDSKASVQAGAGPSAGPGDAAHAQAVPPGYNQHLQVKDSYLTGRALLYFTSVFVSLGVFLFGYDQGVMSGIITGPLFKLYFDDPSRAAIGNMVSILEVGALVSSLMVGRLGEKWGRRRTIRYGSFVFILGGLIQTSATRMLHLIIGRVISGLGVGLLSTIVPIYQSEISPPHNRGKLACIEFTGNIVGYCSSVWVDYGCSYINNNAAWRLPLFIQCVMGLGLFMGTFVIVETPRWLLNHDHDVEGLIVIADLHSDGDVQDRRAHDEFQAIKETVLISRLEGEKKSYSYVFKRYRTRMLIAMSSQMFAQLNGINVISYYAPMVFEQAGWVGRDAILMTGVNGIIYVLSTVLPWHLVDRWGRKPILLMGGMVMGLSLVSISAALYVNIASTPTLVVVFVIVFNAFFGFSWGPIPWLYPVEIAPLSARSAMASASTATNWLCNWLVGIMTPILQEKIKWRLYLIHAASCFLSFWCVLRVYPETAGLRLEDMSSVFDDRSSTFSFHGSITGVDVDAGSRSATTAPANPSLARKPVAAQPSLLDSVHSGVAPPHPVAGQPDLAQNPFVSHSSGQSVGSKRSIASANMLSADALEPPTLGQVLDFKREQIDSRNFKGLMRRGSETVSLIYDKVKRLGKPNDASGEYGTLPDNERPAPAAPPCGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIN4</accession>
    <name>C5DIN4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0E13882p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0E13882g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554082.1">
        <property type="nucleotide sequence ID" value="XM_002554036.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DIN4">
        <property type="interactions" value="196"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIN4"/>
    <dbReference type="GeneID" id="8292251"/>
    <dbReference type="KEGG" id="lth:KLTH0E13882g"/>
    <dbReference type="eggNOG" id="KOG0504">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1162">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2421">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005444_1_0_1"/>
    <dbReference type="InParanoid" id="C5DIN4"/>
    <dbReference type="OMA" id="WTPMEHA"/>
    <dbReference type="OrthoDB" id="197419at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047389">
        <property type="term" value="F:glycerophosphocholine phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046475">
        <property type="term" value="P:glycerophospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14484">
        <property type="entry name" value="SPX_GDE1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057506">
        <property type="entry name" value="C2_GPCPD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051578">
        <property type="entry name" value="GDPD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22958:SF1">
        <property type="entry name" value="GLYCEROPHOSPHOCHOLINE PHOSPHODIESTERASE GPCPD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22958">
        <property type="entry name" value="GLYCEROPHOSPHORYL DIESTER PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25329">
        <property type="entry name" value="C2_GDE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03105">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="SPX" evidence="6">
        <location>
            <begin position="1"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="464"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="564"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="domain" description="GP-PDE" evidence="7">
        <location>
            <begin position="937"/>
            <end position="1277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="335"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="634"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="692"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="341"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="634"/>
            <end position="649"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR23645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1285" mass="142845" checksum="AD2310411542BDFD" modified="2009-07-28" version="1">MKFGKTFPNHQVPEWSHQYVHYKNLKKLIKEITAVQDQLYKQKWSQSNEGGAPVKSRNSTDAEENFLEVPEVKKRLATFFFALDRDIENVNDFYNTQFLEYDRRLRRLLTSAQLADLDAAAQVKRDYSHLRPQAAPIAPDFAEDYTEILGVLIELRSDFRNLKWYAELNKRAFTKILKKLDKKAGTKLQQPYLESRIWPLGISNDTEVSEKLGVINSTLGKISPSIDELQEGPDHEFEGSYAEVGSKDISSPVNVVLQMIEKDDGRSLINELVSMYRSVVFIPKRTLIALLNKAALSQSFQCIDEILEIIPTLGDHSDISGRNFFHHHVIALGKSSKQRKNRESTQDAKNETGDDDTDTPLLAALNVEAAAVPGHNTRLVKAYGPDGVNSNDSPVSLLYILNRLPPHLRSSLLQRDTYKRTPLHYSAQYGLFEVSKIIIESLKEWAAWDAAVPIDSIERWGDSEGFTPMHLAVIGVHPRTVKALTSYGAPSTSLNSPRLLHLATKLNSPELIEVLLSIKGFDVDYTEPETHETALYTAAKLNLRESAAHLLKSGANTEIRECNFGWTPIFAAATEGYYDIAKMLADHGAQYNIFDESGWTPTEHAVLRGHLDVANLLKVDSSVVMRPKLSAKFDREEKVETLESSKKTEVSSTSSPAPERPSQSLYKLSTGSESFISSSSDIEKKTSKHALLKSSLGPSHRKNNDSSKSSHSPPPLPKSFGHSYLQKDQSVVLIRLGGGDSKEKPAVLFNKVPLSKVSSTKLDTALSLVITCPDDTNNKPAVLDLPLDDILDPIHFTIPFKPDSSHALYFDIVPTYGYHAMKIANSSTDLKLKEIHELDGRSASESRDLHSKLGDATEDSQKTKILGRAVALLDKKTMSVGPNRSSLSDSRTVPIIENDTLDVLGTINFEFMIVKPFSHPNVSLGRGETYWKSLVSTRVIGHRGLGKNMNKNNSLQLGENTVESFIAAASLGASYIEFDVQLTKDDIPVIYHDFLVAESGADIPMHALTLEQFLDLNNADKHHRRLDQDLSRRRSMDDSDAAFIQRAIHMRGENGAGIEKTTGEKSSLLGKLFEDRMRLTRTFKKNNFKGNARGHSIASSFVTLKELFKKIPLNVGFNVECKYPMLDEAIEDDIGHITVELNHWCDTVLQVVYDNANGRDIIFSSFHPDVCIMLSLKQPSFPILFLTEGGTKKTVDPRAASLQNAIRFARTWNLLGIVSAAKPVIMAPRLAQVIKSSGLVCVTYGVDNNDPEVAKIEMDAGVDAVIVDSVLAVRRGLTKYSSAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIX0</accession>
    <name>C5DIX0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0E15818p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0E15818g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554168.1">
        <property type="nucleotide sequence ID" value="XM_002554122.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIX0"/>
    <dbReference type="FunCoup" id="C5DIX0">
        <property type="interactions" value="39"/>
    </dbReference>
    <dbReference type="GeneID" id="8292339"/>
    <dbReference type="KEGG" id="lth:KLTH0E15818g"/>
    <dbReference type="eggNOG" id="ENOG502QW4R">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038590_0_0_1"/>
    <dbReference type="InParanoid" id="C5DIX0"/>
    <dbReference type="OMA" id="RKCVICE"/>
    <dbReference type="OrthoDB" id="4076003at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR23731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="438" mass="49792" checksum="190524F6FDA7212A" modified="2009-07-28" version="1">MDEDAVRSSFISQDTHSSLLLMNKSHSDASYGPVSGETPVRWQTGNREFHFNSNSTPSKGTDAPLEDPARKLLRKRRIVRNTLSGAKPRLRSKLSGSAAKLDLLDDQVLTSLPIPPPKEKHYNATESHVNEVQMRIHKDNRRISIAPKRQHSEAESAHPIVLVEDYIPQDPSQLRFAKNRVSVSDLKSKISKSKISRLPIKLKDNRSASAASSLTLTPHINDDDSFANMGMISEDDECVENQNLAFDQHSTETGKTAVADIEHLLEGVLKPSFRGSKDEYLQAFNLEEKIKGCVICEKPLYEISTLLEGRHFREIVCSSCTLRYEETAKILEDYEFETSYDSIDSSRDCSINSEDLLEETEPAIPIANKRFKANTFSPHLINRLQLQIQNIPEKEKSRDEHTVDSKTMLWFIEAKRKLRWRWRVNGLIPQFLAGKRNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJ45</accession>
    <name>C5DJ45_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0F13398p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0F13398g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554771.1">
        <property type="nucleotide sequence ID" value="XM_002554725.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJ45"/>
    <dbReference type="FunCoup" id="C5DJ45">
        <property type="interactions" value="54"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJ45"/>
    <dbReference type="GeneID" id="8292995"/>
    <dbReference type="KEGG" id="lth:KLTH0F13398g"/>
    <dbReference type="eggNOG" id="KOG0691">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025145_3_1_1"/>
    <dbReference type="InParanoid" id="C5DJ45"/>
    <dbReference type="OMA" id="RPALMDK"/>
    <dbReference type="OrthoDB" id="552049at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016558">
        <property type="term" value="P:protein import into peroxisome matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000028">
        <property type="entry name" value="DnaJ domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026894">
        <property type="entry name" value="DnaJ_X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052814">
        <property type="entry name" value="Peroxisomal_DnaJ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45006">
        <property type="entry name" value="DNAJ-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45006:SF1">
        <property type="entry name" value="DNAJ-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14308">
        <property type="entry name" value="DnaJ-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="3">
        <location>
            <begin position="6"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="114"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="119"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="160"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR24334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="419" mass="46993" checksum="CD5A07345F4A78A3" modified="2009-07-28" version="1">MVVDTTYYDLLGVSPDAKSIDIKKAYRKKSVQEHPDKNPNDPTATERFQAISQAYQVLSKDDLRAKYDKFGKEEAVPKEGFEDAAEQFSMIFGGDAFASYIGELTLLKNLQKTGELSAEDEKDKEAENEASEEKKDATTAPSEAANMDQGGAKVLPPSESLDSMSSTQNGEKKDKKKSKLEAFEEEQKIEKEKSIQELSKLLSDRLSILTESEYNEACKESFARKFEEEANMLKMESFGLDILHTIGDVYYEKGQIFLKSQLVWGLGGMFHSFKAKSGVVMDTLKTVSAALDAQNTMQELEKLKAVAESNEVLRDDKGQEILKPSVEELAQLEQLLMGKVLSAAWHGSKFEIMSTLRSVCDKVLEDAKVDKTTLVRRAETLIILGKVFRKTFRTKVEQEEAQVFEELVAEATKKKSKHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJB1</accession>
    <name>C5DJB1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Heat shock transcription factor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Heat shock factor protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0F14982g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">DNA-binding transcription factor that specifically binds heat shock promoter elements (HSE) and activates transcription.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homotrimer. Homotrimerization increases the affinity of HSF1 to DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the HSF family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554837.1">
        <property type="nucleotide sequence ID" value="XM_002554791.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJB1"/>
    <dbReference type="FunCoup" id="C5DJB1">
        <property type="interactions" value="463"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJB1"/>
    <dbReference type="GeneID" id="8293066"/>
    <dbReference type="KEGG" id="lth:KLTH0F14982g"/>
    <dbReference type="eggNOG" id="KOG0627">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_362926_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJB1"/>
    <dbReference type="OMA" id="PYLMMNP"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
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        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:protein-DNA complex"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0003700">
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:sequence-specific DNA binding"/>
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        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="HSF_DNA-bd"/>
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        <property type="entry name" value="WH-like_DNA-bd_sf"/>
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        <property type="entry name" value="WH_DNA-bd_sf"/>
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    <dbReference type="PANTHER" id="PTHR10015:SF396">
        <property type="entry name" value="FLOCCULATION SUPPRESSION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00056">
        <property type="entry name" value="HSFDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS00434">
        <property type="entry name" value="HSF_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="12">
        <location>
            <begin position="112"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="66"/>
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    </feature>
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        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
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        <location>
            <begin position="189"/>
            <end position="217"/>
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        <location>
            <begin position="266"/>
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    </feature>
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        <location>
            <begin position="424"/>
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    </feature>
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        <location>
            <begin position="502"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="642"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="741"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="19"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="189"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="272"/>
            <end position="292"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="293"/>
            <end position="306"/>
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        <location>
            <begin position="307"/>
            <end position="327"/>
        </location>
    </feature>
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        <location>
            <begin position="328"/>
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    </feature>
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        <location>
            <begin position="450"/>
            <end position="463"/>
        </location>
    </feature>
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        <location>
            <begin position="506"/>
            <end position="519"/>
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    </feature>
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        <location>
            <begin position="520"/>
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    </feature>
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        <location>
            <begin position="652"/>
            <end position="661"/>
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        <location>
            <begin position="680"/>
            <end position="695"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="749"/>
            <end position="758"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="791"/>
            <end position="803"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="817"/>
            <end position="828"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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            <dbReference type="ARBA" id="ARBA00023163"/>
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            <dbReference type="ARBA" id="ARBA00023242"/>
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            <dbReference type="ARBA" id="ARBA00059868"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00062447"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00068818"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00084017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR24400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="828" mass="90253" checksum="21BBD4D7BD7FB54C" modified="2009-07-28" version="1">MAAGNAAQEEASKSRDASRSANSDAARSANSEEPGASGVRGGAGSAADASNSAGGGPGAGSSTNSSASYSNTAFIHKLYSMLEDPEMDELIWWSPPQTSFLIRPTERFGKALATYFKHTNVASFVRQLNMYGFHKVNDHKTHAASKNGAAKTDDPASKSENTTSIWEFKHSSSAFRRGDMESLKLIKRRSSRYQASARKRSTSAASTPLEPTMTEHSPDQLLVPHMHAASADLPHPYGYTPAPQSFPAQPLPSQVLYAPVDYFATTHQAQQQHHHQQQPQQHYHHHHHHHHQQQQQQTQQPQQQQPQPQPQPQPQSQPQPQPQPQPQPHQQSQQQQPIYQVPQHQSTQSLSHHQSISQQAQQSRPQINTKVSDVLHGVQLAKFDNNAYQNQYEAAVEDLRNSNMDMVRLLDLVHNLVTLTPQLAAASSGRPSEGIKRSTTPVSGHPNPSEMGTSPRDTPSQQQCMDQIMSEIARLKNSTLYRLQRSTMLQQTVRPVHSFSEYPMTSSSAIPAASRPPLSNQTSLSSPALSQLTNAQPAPLPQMSHAMYYPPAYGPSVYPGTPGPQSDYFGTTAKANSGSMPYLMMNPFEKKTSTSSSGNRNMSVLMDPLAPAPIVQALPGVSTSASPNPNQQIAQQHGYFAGHSTSNLPLADSRKNSRNETKSIPTSNPDGARDLKQSNRRPQSPSVAETPTRATANKPAGSAVSTMTYKPYFPYGTVPGAGPTSPMRASVAQYSQIAAPGPLPHYQKAHQQNPSHTYPQYRGPSPLNPNTAEAPDQDKIVKPMPISPAVRDQEASAASRDENSGSSSRLYALLNYDSKEQSSKKMKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJB8</accession>
    <name>C5DJB8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0F15136p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0F15136g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554844.1">
        <property type="nucleotide sequence ID" value="XM_002554798.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJB8"/>
    <dbReference type="FunCoup" id="C5DJB8">
        <property type="interactions" value="1228"/>
    </dbReference>
    <dbReference type="GeneID" id="8293073"/>
    <dbReference type="KEGG" id="lth:KLTH0F15136g"/>
    <dbReference type="eggNOG" id="KOG0254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_30_5_1"/>
    <dbReference type="InParanoid" id="C5DJB8"/>
    <dbReference type="OMA" id="GFNAFMY"/>
    <dbReference type="OrthoDB" id="5290825at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005366">
        <property type="term" value="F:myo-inositol:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904679">
        <property type="term" value="P:myo-inositol import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050814">
        <property type="entry name" value="Myo-inositol_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020:SF12">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="116"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="146"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="175"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="203"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="332"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="367"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="398"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="422"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="457"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="490"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="47"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="301"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="308"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR24407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="537" mass="58408" checksum="50F9FDB6F66A8A26" modified="2009-07-28" version="1">MATEGPSSTEDDEVYSDLYSAYSQLTDTTAKDIDHRRYPLNCKTVKIYLVASIGGLLFGYDTGVIAGVLLGLRPQDLGLDNLGDFNKELITGITGIGSVLGSIVAFPTADYLGRKIALALCSIIFCISAVIMALSTSLFTLVTGRLVVGVAVGIAAQCVPVYLSEVSPSSVRGSVMTINSSAITGGQLIACVVAWILSGKPHSWRYLFGLSAIPAIVLMCLLKFIPESPRWLLLGDKPQLAREALEVIYADASDLEISNKLLKMVRDLTKLRCYQDESQPFISRAPGINKRLSSNSSLDYASHARSIPPSNRSVQRPVSSKKHKMEPRTRRALFVGCILMFFQQATGFNAFIYYSPVILARIGVSNPLLPAIAIAAVNFIFTGVAMQAVDRWGRRNTLLYTIWIMALGLIVGSVGLQYGNPALFLASLLVYVAGYASGLGSIPWLSVEFLPLNQRSLGASVIACTNWLTNFGISISFLSTMTFVGSQASMLIFAAITTVSWLFVCFCYPEVKGLSLEEIGQIFEEGVDIHYVYRKYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJC0</accession>
    <name>C5DJC0_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Elongation factor 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0F15180g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554846.1">
        <property type="nucleotide sequence ID" value="XM_002554800.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJC0"/>
    <dbReference type="FunCoup" id="C5DJC0">
        <property type="interactions" value="1346"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJC0"/>
    <dbReference type="GeneID" id="8293075"/>
    <dbReference type="KEGG" id="lth:KLTH0F15180g"/>
    <dbReference type="eggNOG" id="KOG0469">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002794_11_2_1"/>
    <dbReference type="InParanoid" id="C5DJC0"/>
    <dbReference type="OMA" id="ASWNTEN"/>
    <dbReference type="OrthoDB" id="364892at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01681">
        <property type="entry name" value="aeEF2_snRNP_like_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04096">
        <property type="entry name" value="eEF2_snRNP_like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01885">
        <property type="entry name" value="EF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16261">
        <property type="entry name" value="EF2_snRNP_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03700">
        <property type="entry name" value="EF2_snRNP_like_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000010">
        <property type="entry name" value="Translation elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000006">
        <property type="entry name" value="Translation elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.240:FF:000003">
        <property type="entry name" value="Translation elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.870:FF:000002">
        <property type="entry name" value="Translation elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000058">
        <property type="entry name" value="Translation elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.870">
        <property type="entry name" value="Elongation Factor G (Translational Gtpase), domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041095">
        <property type="entry name" value="EFG_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005517">
        <property type="entry name" value="Transl_elong_EFG/EF2_IV"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908:SF10">
        <property type="entry name" value="EUKARYOTIC TRANSLATION ELONGATION FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908">
        <property type="entry name" value="TRANSLATION ELONGATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14492">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03764">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00889">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="9">
        <location>
            <begin position="17"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR24409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="842" mass="93354" checksum="D60E10364012CBDD" modified="2009-07-28" version="1">MVAFTVDQMRSLMDKVTNVRNMSVIAHVDHGKSTLTDSLVQRAGIISAAKAGEARFTDTRKDEQERGITIKSTAISLYSEMTEDDVKDIKQKTIGNSFLINLIDSPGHVDFSSEVTAALRVTDGALVVVDTVEGVCVQTETVLRQALGERIKPVVVINKVDRALLELQVTKEDLYQSFSRTVESVNVIVSTYADEVLGDVQVFPQQGTVAFGSGLHGWAFTIRQFANRYSKKFGVDRQKMMDRLWGDSYFNPKTKKWTNKEVDADGKPLERAFNMFVLDPIFRLFAAIMNFKKDEIPVLLEKLEINLKGDEKDLEGKALLKVVMRKFLPAADALLEMIVMHLPSPVTAQNYRAEQLYEGPSDDPACVAIKNCDPTSDLMLYVSKMVPTSDKGRFYAFGRVFAGTVKSGQKIRIQGPNYVPGKKDDLFLKAVQRVVLMMGRFVEPIDDCPAGNIVGLVGIDQFLLKTGTLTTYESAHNMKVMKFSVSPVVQVAVEVKNANDLPKLVEGLKRLSKSDPCVLCYMSESGEHIVAGTGELHLEICLSDLENDHAGIPLKISPPVVAYRETVEGESSQVALSKSPNKHNRIYLKAEPMDEECSLAIEDGKINPRDDFKARARVMADEYGWDVTDARKIWCFGPDGNGPNVVVDQTKAVQYLHEIKDSVVAAFQWASKEGPIFGEQMRSVRVNILDVTLHADAIHRGGGQIIPTMRRATYAGFLLAEPKIQEPVFLVEIQCPEQAVGGIYSVLNKKRGQVVSEEQRPGTPLFTVKAYLPVNESFGFTGQLRQATGGQAFPQMVFDHWATLSADPLDPSSKAGEIVAAARKRHGMKEEVPGWQEYYDKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJG9</accession>
    <name>C5DJG9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Needs CLA4 to survive protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">UBA4</name>
        <name evidence="15" type="ordered locus">KLTH0F16346g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="13">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="13">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">In the N-terminal section; belongs to the HesA/MoeB/ThiF family. UBA4 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554895.1">
        <property type="nucleotide sequence ID" value="XM_002554849.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJG9"/>
    <dbReference type="FunCoup" id="C5DJG9">
        <property type="interactions" value="902"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJG9"/>
    <dbReference type="GeneID" id="8293128"/>
    <dbReference type="KEGG" id="lth:KLTH0F16346g"/>
    <dbReference type="eggNOG" id="KOG2017">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013325_1_2_1"/>
    <dbReference type="InParanoid" id="C5DJG9"/>
    <dbReference type="OMA" id="IPDVGMD"/>
    <dbReference type="OrthoDB" id="10261062at2759"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070566">
        <property type="term" value="F:adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004792">
        <property type="term" value="F:thiosulfate-cyanide sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042292">
        <property type="term" value="F:URM1 activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032447">
        <property type="term" value="P:protein urmylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002143">
        <property type="term" value="P:tRNA wobble position uridine thiolation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00757">
        <property type="entry name" value="ThiF_MoeB_HesA_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000014">
        <property type="entry name" value="Adenylyltransferase and sulfurtransferase MOCS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000033">
        <property type="entry name" value="Adenylyltransferase and sulfurtransferase MOCS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03049">
        <property type="entry name" value="MOCS3_Uba4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028885">
        <property type="entry name" value="MOCS3/Uba4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF102">
        <property type="entry name" value="ADENYLYLTRANSFERASE AND SULFURTRANSFERASE MOCS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 13" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 13" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9 13" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Rhodanese" evidence="14">
        <location>
            <begin position="340"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate; for adenylyltransferase activity" evidence="13">
        <location>
            <position position="222"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine persulfide intermediate; for sulfurtransferase activity" evidence="13">
        <location>
            <position position="393"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="102"/>
            <end position="106"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="163"/>
            <end position="164"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00075323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR24458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="436" mass="48580" checksum="2B5EBF2B2349D580" modified="2009-07-28" version="1">MTTDNIPNEVLLELEALRRENEALKQKLSSQTELPMSLEEFRRYGRQMIVEDTRGVEGQIKLRNAKVLVIGAGGLGCPCLPYLVGAGVGQVGVVDNDVIDTSNLHRQVLHDSTKVGMLKCESAKDVLRKLNPHVQIKTYPVRLNYSNAFSIFQGYDIVLDCTDTPLTRYLVSDVAVCLGMTVVSASGLGSEGQLSILNFASVGPCYRCFYPVPPPPNAVSSCQEGGVIGPCIGLVGVMMAVETLKIILDIYTLDNFKPFLIQYSGLPNQTLRSFKMRGRQPSCKACGEEKLITRDTIEAGEVNYEAFCGARNYNVCSPEERINVQEFEDSISSNEKLSQILLDVRPHHHYKISHLPNTFNLTVKELRDMEGDMSLLQNEIPEIHNGSEVLVMCRYGNDSQLATRILKDKFNIMKVKDIRGGFFKYIDDINPSLPKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJP4</accession>
    <name>C5DJP4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0F18062p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0F18062g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554970.1">
        <property type="nucleotide sequence ID" value="XM_002554924.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJP4"/>
    <dbReference type="STRING" id="559295.C5DJP4"/>
    <dbReference type="GeneID" id="8293205"/>
    <dbReference type="KEGG" id="lth:KLTH0F18062g"/>
    <dbReference type="eggNOG" id="KOG0725">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_1_1"/>
    <dbReference type="InParanoid" id="C5DJP4"/>
    <dbReference type="OMA" id="WAPKVRI"/>
    <dbReference type="OrthoDB" id="1888931at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050085">
        <property type="term" value="F:mannitol 2-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050664">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044281">
        <property type="term" value="P:small molecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05352">
        <property type="entry name" value="MDH-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000090">
        <property type="entry name" value="NADP-dependent mannitol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43008">
        <property type="entry name" value="BENZIL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43008:SF13">
        <property type="entry name" value="L-XYLULOSE REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR24533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="283" mass="30392" checksum="31447AC6462E04A7" modified="2009-07-28" version="1">MSKTIATSLKTPQKAPQLPDNVMDMFSLKGKVAAVSGASSGIGYDVAIAYMQAGADVAMWYNSNPHITELVDELSQKYGVKVKAYKCAVTSSKEVAETIEQIKKDFGGRIDILVGNAGVAWTEGPLVDLAETDEEKCDAEWNKVIDVDLNGIYRLAKNIGKVFKAQGHGSFIITSSMSGHIVNVPQMQTAYNAAKAGVLHMARSLAVEWAGFARVNTVSPGYIATEITNFAEDSLKDKWHSLTPMGREALPRELIGAYVYLASGASTYTTGSDIVVDGGYCCM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DK40</accession>
    <name>C5DK40_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0F01562p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0F01562g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the nitroreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554278.1">
        <property type="nucleotide sequence ID" value="XM_002554232.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DK40"/>
    <dbReference type="FunCoup" id="C5DK40">
        <property type="interactions" value="40"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DK40"/>
    <dbReference type="GeneID" id="8292469"/>
    <dbReference type="KEGG" id="lth:KLTH0F01562g"/>
    <dbReference type="eggNOG" id="ENOG502RYI9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073125_1_0_1"/>
    <dbReference type="InParanoid" id="C5DK40"/>
    <dbReference type="OMA" id="EMFENHT"/>
    <dbReference type="OrthoDB" id="2138173at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02140">
        <property type="entry name" value="Frm2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.109.10:FF:000001">
        <property type="entry name" value="Nitroreductase family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.109.10">
        <property type="entry name" value="NADH Oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033877">
        <property type="entry name" value="Frm2/Hbn1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029479">
        <property type="entry name" value="Nitroreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000415">
        <property type="entry name" value="Nitroreductase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43035">
        <property type="entry name" value="FATTY ACID REPRESSION MUTANT PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43035:SF1">
        <property type="entry name" value="FATTY ACID REPRESSION MUTANT PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00881">
        <property type="entry name" value="Nitroreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55469">
        <property type="entry name" value="FMN-dependent nitroreductase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitroreductase" evidence="8">
        <location>
            <begin position="13"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR23841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="195" mass="21681" checksum="E439E41610D6A270" modified="2009-07-28" version="1">MSPAAAPFLEALVARRTIYALKPELPSGVELKDVKDIVQTIIKHTPTSFNTQGNRAVILTGESHRKVWDHVYNSIPIENFKKRPLSARDEAFGTVVFMVNNQQTKEMQQNFPAWADLFPEFVAHSSGSAQISAWTALEKIGLGAHLQHFNELVASALPEDAIKEEWVVQGQLVFGLPDAPAGEKTFIENEVKVFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>C5DK78</accession>
    <name>C5DK78_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">KLTH0F02420p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0F02420g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554316.1">
        <property type="nucleotide sequence ID" value="XM_002554270.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DK78"/>
    <dbReference type="FunCoup" id="C5DK78">
        <property type="interactions" value="139"/>
    </dbReference>
    <dbReference type="GeneID" id="8292508"/>
    <dbReference type="KEGG" id="lth:KLTH0F02420g"/>
    <dbReference type="eggNOG" id="KOG1286">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007946_12_1_1"/>
    <dbReference type="InParanoid" id="C5DK78"/>
    <dbReference type="OMA" id="LFKALWY"/>
    <dbReference type="OrthoDB" id="3900342at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000006">
        <property type="entry name" value="General amino acid permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004840">
        <property type="entry name" value="Amino_acid_permease_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004762">
        <property type="entry name" value="Amino_acid_permease_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00913">
        <property type="entry name" value="2A0310"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF19">
        <property type="entry name" value="LYSINE-SPECIFIC PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00218">
        <property type="entry name" value="AMINO_ACID_PERMEASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="124"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="147"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="174"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="205"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="233"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="259"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="355"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="408"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="453"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="484"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="525"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="558"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="10">
        <location>
            <begin position="123"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="91"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="28"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR23879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="621" mass="68483" checksum="79A085F8AFE9479D" modified="2009-07-28" version="1">MARKKEACAPCASPGLYCKSSGEAKRPVSSSRQLQSTEQSIHQRCCSVPKHPGMTEKNSLHSRARRGSGATAGEPKLKAHSDDFEMYVYSQDEPSTDPLGSDTESGEGDMQEGKVKRALKPRHVSMIALGGTIGTGLFVGIASPLSYSGPVGALIAYIFMGTVVYFVTQSMGEMATFIPVTSSITVFSSRFLSPAFGVANGYMYWFNWAITFAVELSVTGQVIEYWTEAVPRAAWIAIFWVLVTLANFFPVQFYGEVEFWVASIKVLAIVGYLIYALVIVCGGSKQGPIGFRYWRNPGPWGAGIISKDKDEGRFLGWVASLINASFTYQGTELVGITAGEAANPRRTVPRAINKVFFRILFFYILSLFFVGLLVPYNHSSLDKTTTVIASSPFVISIQNAGTRALPDIFNAVVLVTIISAANSNVYVGSRVLFSLAHTGIAPKVFSSVTPQGVPFMGVIVTSLLGLLAFLVVNHNANEAFNWLINISTLAGLCAWLFISISHIRFMQCLKQRGISRDDLPFKSKLMPYGAYYAAFWVTVIIFVQGFQAFSPHFKVTEFFTSYISLMLLVVVFCGAQLFYRCRFFNRLEDIDIDSDRREADALIWEDDEPTTLWGKFWTAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKH1</accession>
    <name>C5DKH1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0F04620p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0F04620g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EBP2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554409.1">
        <property type="nucleotide sequence ID" value="XM_002554363.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKH1"/>
    <dbReference type="FunCoup" id="C5DKH1">
        <property type="interactions" value="437"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKH1"/>
    <dbReference type="GeneID" id="8292604"/>
    <dbReference type="KEGG" id="lth:KLTH0F04620g"/>
    <dbReference type="eggNOG" id="KOG3080">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036007_3_1_1"/>
    <dbReference type="InParanoid" id="C5DKH1"/>
    <dbReference type="OMA" id="DAHKGRD"/>
    <dbReference type="OrthoDB" id="443772at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008610">
        <property type="entry name" value="Ebp2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13028">
        <property type="entry name" value="RRNA PROCESSING PROTEIN EBNA1-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13028:SF0">
        <property type="entry name" value="RRNA-PROCESSING PROTEIN EBP2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05890">
        <property type="entry name" value="Ebp2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="16"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="275"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="343"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="16"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="78"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="95"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="112"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="125"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="275"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="376"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="390"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR23972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="406" mass="47077" checksum="5EAABE9A44429861" modified="2009-07-28" version="1">MAGFKLKELLKHQKQIEKEDKKEAVKKDRKSKELEKLNDEPVVVSHEDAVEDQKVATADEKVADKPQEEYQSQALSRKERRKLKKQQKKSHPTEDENNDSEEEEEGEGDEENSSKRLQLDKLEMSEPEDEEEEDEENEDQEAQSEDEEEKDVPLSDVEFDSDADVVPFQKVTVNNSNAIKHALERVQLPWKKHSFQEHQSITSEGNTDETIKDIYDDTQRELALYKQSLDAVLQARQKLKTLKVPFKRPLDYFAEMVKSDEHMDKLKSKLVSEASDRKAREDARKQRQLKKFGKQVQVATLQSRQKEKRETLEKIKSLKKKRKHNEIDDSAFDVGIEEAATEKGYDKRQKPNGKRLAKDAKYGKGGMKRFKRKNDAQSSSDITGFSSNKMKGKPSRPGKSRRAKRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKK2</accession>
    <name>C5DKK2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0F05412p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0F05412g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554440.1">
        <property type="nucleotide sequence ID" value="XM_002554394.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKK2"/>
    <dbReference type="FunCoup" id="C5DKK2">
        <property type="interactions" value="49"/>
    </dbReference>
    <dbReference type="GeneID" id="8292638"/>
    <dbReference type="KEGG" id="lth:KLTH0F05412g"/>
    <dbReference type="eggNOG" id="ENOG502R2Z2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034560_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKK2"/>
    <dbReference type="OMA" id="CIREYMT"/>
    <dbReference type="OrthoDB" id="2014201at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="375" mass="42124" checksum="4855B3D558CB9E19" modified="2009-07-28" version="1">MDLPDDLASAVRKSWSESAQPLSVLQGTDSPISTPRGTPKSSGRGSIPTSMDEPTVDYQLLKHHYAISDDGRRPSMQEDLMDVLQEMEFSNEQRPQAMRRGSIEEVSKIRQWLNPRSSFSGASTNEASTENTGATKCWVTTIETQDDFLPILVLNYSFKKSCSRYKLIVLYTEQTEMVASRLRDYGVETRRASDIAPILFSPQNQMGTADSRLSRWTMLFPFVSLTNDFELVCYLSPRALVLSNIDELLDSDAVAAEIDNETCVLLSNHMGARPTIMVLRPNKEIDACIREYMTVYLNAQNNGKWAKLQAADGSSVLRELFEDSWGLVAADFCHSGPGHIQPNAKIVECTTTQPWNLSYNDEASVLWRRAWSELI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2010-10-05" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKM2</accession>
    <name>AIM23_LACTC</name>
    <protein>
        <recommendedName>
            <fullName>Altered inheritance of mitochondria protein 23, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">AIM23</name>
        <name type="ordered locus">KLTH0F05852g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the AIM23 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554460.1">
        <property type="nucleotide sequence ID" value="XM_002554414.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKM2"/>
    <dbReference type="SMR" id="C5DKM2"/>
    <dbReference type="FunCoup" id="C5DKM2">
        <property type="interactions" value="48"/>
    </dbReference>
    <dbReference type="GeneID" id="8292658"/>
    <dbReference type="KEGG" id="lth:KLTH0F05852g"/>
    <dbReference type="eggNOG" id="ENOG502RY27">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057910_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKM2"/>
    <dbReference type="OMA" id="KVSWQIS"/>
    <dbReference type="OrthoDB" id="3996489at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029427">
        <property type="entry name" value="AIM23"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14877">
        <property type="entry name" value="mIF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0496">Mitochondrion</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0809">Transit peptide</keyword>
    <feature type="transit peptide" description="Mitochondrion" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000399537" description="Altered inheritance of mitochondria protein 23, mitochondrial">
        <location>
            <begin position="19"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="339"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="343"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="378" mass="43537" checksum="639BB8D7DD720875" modified="2009-07-28" version="1" precursor="true">MKLIASVLNPVHNFDARSTAMLSLLTFKNCFLSNGRVLIFQLKPCRKLQTTRFVLAESVASNNDILFNATASMRKNKDAAKLMTDRIHYVSPAKRKQTRTPSTGHQNRKRTTLKWNTGSERAQAAANFTLRQVFKMNERGVVKVVNQTTNKLEETSILIWAGELDLQKHGLAIVDVEQRGTFSIPLVKLVESKTALKKYSDELAKQKEEELTRLGFSSKRTGRKNENDSNEDNLKQIKVSWQISDADLNKQKANEIISQLKKGYKVFLYMNGKDALNKSNWAEDITDEEPAHTRKHSDRELERRQHIVEQLQVIVNEFSLSPTVEGSIETRLIMKLSPKPVASKKEDRMALKEQRKRERQEKLERKLEKKKLRNSENY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKM3</accession>
    <name>C5DKM3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="26">Pyrimidine-specific carbamoyl phosphate synthase-aspartate carbamoyl transferase</fullName>
            <ecNumber evidence="6">2.1.3.2</ecNumber>
            <ecNumber evidence="5">3.5.1.2</ecNumber>
            <ecNumber evidence="20">6.3.4.16</ecNumber>
            <ecNumber evidence="4">6.3.5.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="30" type="ordered locus">KLTH0F05874g</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="30 31" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="31">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="25">Multifunctional protein that encodes the first 2 enzymatic activities of the de novo pyrimidine pathway: carbamoylphosphate synthetase (CPSase; EC 6.3.5.5) and aspartate transcarbamylase (ATCase; EC 2.1.3.2). The CPSase-function is accomplished in 2 steps, by a glutamine-dependent amidotransferase activity (GATase) that binds and cleaves glutamine to produce ammonia, followed by an ammonium-dependent carbamoyl phosphate synthetase, which reacts with the ammonia, hydrogencarbonate and ATP to form carbamoyl phosphate. The endogenously produced carbamoyl phosphate is sequestered and channeled to the ATCase active site. ATCase then catalyzes the formation of carbamoyl-L-aspartate from L-aspartate and carbamoyl phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20013"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:32814"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="EC" id="2.1.3.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>hydrogencarbonate + L-glutamine + 2 ATP + H2O = carbamoyl phosphate + L-glutamate + 2 ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:18633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>hydrogencarbonate + NH4(+) + 2 ATP = carbamoyl phosphate + 2 ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:18029"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="19">In the 2nd section; belongs to the CarB family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="16">In the 3rd section; belongs to the metallo-dependent hydrolases superfamily. DHOase family. CAD subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="17">In the C-terminal section; belongs to the aspartate/ornithine carbamoyltransferase superfamily. ATCase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="18">In the N-terminal section; belongs to the CarA family.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.2" evidence="6"/>
    <dbReference type="EC" id="3.5.1.2" evidence="5"/>
    <dbReference type="EC" id="6.3.4.16" evidence="20"/>
    <dbReference type="EC" id="6.3.5.5" evidence="4"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554461.1">
        <property type="nucleotide sequence ID" value="XM_002554415.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DKM3">
        <property type="interactions" value="1914"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKM3"/>
    <dbReference type="MEROPS" id="C26.956"/>
    <dbReference type="GeneID" id="8292659"/>
    <dbReference type="KEGG" id="lth:KLTH0F05874g"/>
    <dbReference type="eggNOG" id="KOG0370">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000513_2_1_1"/>
    <dbReference type="InParanoid" id="C5DKM3"/>
    <dbReference type="OMA" id="WSPFNGK"/>
    <dbReference type="OrthoDB" id="1924069at2759"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00115"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005951">
        <property type="term" value="C:carbamoyl-phosphate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004070">
        <property type="term" value="F:aspartate carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004087">
        <property type="term" value="F:carbamoyl-phosphate synthase (ammonia) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004088">
        <property type="term" value="F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01744">
        <property type="entry name" value="GATase1_CPSase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01423">
        <property type="entry name" value="MGS_CPS_I_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1370:FF:000002">
        <property type="entry name" value="Aspartate carbamoyltransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000025">
        <property type="entry name" value="Bifunctional pyrimidine biosynthesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1370:FF:000005">
        <property type="entry name" value="CAD protein-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000011">
        <property type="entry name" value="CAD protein-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000004">
        <property type="entry name" value="Carbamoyl-phosphate synthase (glutamine-hydrolyzing)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.20:FF:000002">
        <property type="entry name" value="Carbamoyl-phosphate synthase 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1030.10:FF:000001">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000036">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000001">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000001">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000002">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1380:FF:000009">
        <property type="entry name" value="Carbamoyl-phosphate synthase, large subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1370">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.20">
        <property type="entry name" value="Carbamoyl-phosphate synthase small subunit, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1030.10">
        <property type="entry name" value="Carbamoyl-phosphate synthetase, large subunit oligomerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1380">
        <property type="entry name" value="Methylglyoxal synthase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00001">
        <property type="entry name" value="Asp_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01209">
        <property type="entry name" value="CPSase_S_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006132">
        <property type="entry name" value="Asp/Orn_carbamoyltranf_P-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006130">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036901">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002082">
        <property type="entry name" value="Asp_carbamoyltransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006131">
        <property type="entry name" value="Asp_carbamoyltransf_Asp/Orn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036897">
        <property type="entry name" value="CarbamoylP_synth_lsu_oligo_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006274">
        <property type="entry name" value="CarbamoylP_synth_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002474">
        <property type="entry name" value="CarbamoylP_synth_ssu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036480">
        <property type="entry name" value="CarbP_synth_ssu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005483">
        <property type="entry name" value="CPSase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035686">
        <property type="entry name" value="CPSase_GATase1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006275">
        <property type="entry name" value="CPSase_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005480">
        <property type="entry name" value="CPSase_lsu_oligo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058047">
        <property type="entry name" value="CPSase_preATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011607">
        <property type="entry name" value="MGS-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036914">
        <property type="entry name" value="MGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00670">
        <property type="entry name" value="asp_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01369">
        <property type="entry name" value="CPSaseII_lrg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01368">
        <property type="entry name" value="CPSaseIIsmall"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002032">
        <property type="entry name" value="PRK00856.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003671">
        <property type="entry name" value="PRK05294.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009455">
        <property type="entry name" value="PRK12815.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009475">
        <property type="entry name" value="PRK12838.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405:SF5">
        <property type="entry name" value="CAD PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405">
        <property type="entry name" value="CARBAMOYLTRANSFERASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25596">
        <property type="entry name" value="CPSase_L_D1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02787">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00988">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02142">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00185">
        <property type="entry name" value="OTCace"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02729">
        <property type="entry name" value="OTCace_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00100">
        <property type="entry name" value="AOTCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00101">
        <property type="entry name" value="ATCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00098">
        <property type="entry name" value="CPSASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00099">
        <property type="entry name" value="CPSGATASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01096">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01097">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00851">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53671">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48108">
        <property type="entry name" value="Carbamoyl phosphate synthetase, large subunit connection domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52021">
        <property type="entry name" value="Carbamoyl phosphate synthetase, small subunit N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52335">
        <property type="entry name" value="Methylglyoxal synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00097">
        <property type="entry name" value="CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51855">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 27" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0436">Ligase</keyword>
    <keyword evidence="15" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="11 27" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ATP-grasp" evidence="28">
        <location>
            <begin position="562"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="28">
        <location>
            <begin position="1099"/>
            <end position="1290"/>
        </location>
    </feature>
    <feature type="domain" description="MGS-like" evidence="29">
        <location>
            <begin position="1356"/>
            <end position="1507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00043968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00043979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00043984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00043998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00044063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00047359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00048816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00049534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00058513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00081752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS51855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="EMBL" id="CAR24024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="2220" mass="245658" checksum="0BABF0C9C4CFA3CD" modified="2009-07-28" version="1">MSIITPTGPIAPPMESTGDRLVTLELQDGVAVQGYSFGAPKSAAGELVFQTGMVGYPESVTDPSYEGQILVITFPLVGNYGVPDRNLQDELVESLPRYFESSRIHIAGLVIAHYTDQYSHWLANSSLAAWLKEEGIPAVYGIDTRALTKHLRDSGSMLGRLALQKSGSDFVRATSAEWRDSFEIPDWVDPNVQNLVAKVSTSEPHLYTPPSDFDVSNLQKGPDGKTLRILAIDVGMKFNQIRCFVKRGVELLVVPWDYDFTKEQYDGLFISNGPGDPSVLSDLNVKLTKVLESKKTPVFGICLGHQLLARASGASTLKLKFGNRGHNIPCTSTISGKCYITSQNHGFAVDVDTLTTGWKPLFVNANDGSNEGIYHTELPYFSVQFHPESTPGPRDTEFLFDVFIQSVKQFKHTGQIKPVEFPGGKLEDNLAAHPRVEAKKVLVLGSGGLSIGQAGEFDYSGSQAIKALKEEGIYTILINPNIATIQTSKGLADKVYFLPVTAEFVRKVILHERPDAIYVTFGGQTALSVGIEMKDEFEALGVKVLGTPIDTVITTEDRELFSNAMDEINEKCAKSKAASTVEEALEAVKEIGFPVICRSAFALGGLGSGFANNEKELVDLCNVAFASSPQVLVERSMKGWKEVEYEVVRDAFDNCITVCNMENFDPLGIHTGDSIVVAPSQTLSDEDYNMLRTTAVNVIRHLGVVGECNIQYALNPFSKEYCIIEVNARLSRSSALASKATGYPLAYTAAKLGLNIPLNEVKNSVTQSTCACFEPSLDYCVVKMPRWDLKKFNRVSTALSSSMKSVGEVMSIGRTFEEAIQKAIRSTEYVNLGFNETDMDIDIDYELSHPTDERIFAIANAFSNLNYSVEKVWQLSNIDKWFLTKLYDLVAFAKKIESYGAKENLPPLVLKQAKQLGFDDRQIAKFINSNEVAIRRLRKECGITPFVKQIDTVAAEFPAHTNYLYMTYNAATHDLSFNDHGVMVLGSGVYRIGSSVEFDWCAVTAVRTLRKNNIKSIMVNYNPETVSTDYDEADRLYFETISLERILDIYEIETSAGVVVSMGGQTSNNIAMSLHRENVKILGTTPEMIDSAENRYKFSRMLDQIGVDQPAWKELTSIDEAEKFADAVSYPVLVRPSYVLSGAAMNTVYSKDDLASYLNQAVEVSREYPVVITKYIENAKEIEMDAVAKNGELIMHVVSEHVENAGVHSGDATLIVPPQDLDPETVERIVVATAKIGKALKITGPFNIQFIAKNNEIKVIECNVRASRSFPFISKVVNVNLIEMATKAIMGLPVTPYPTEKLPEDYVAIKVPQFSFPRLAGADPVLGVEMASTGEVATFGHSKYEAYLKSLLSTGFKLPKKNVLLSIGSFKEKQEMLPSVKKLYNMGYKLFATAGTADFISEHGIPVQYLEVLNEGDDEKSEYSLTQHLANNKIDLYINLPSANRFRRPASYVSKGYQTRRMAVDYSVPLVTNVKCAKLLIEAISRNLNLEVSERDAQTSHRTVTIPGLINISAYMPHISNVVQGPEEMKEITRLSAESGFTYSQIMPKSTRGPVITNEQSIKVANSVAQESAYTDFGFTVAATAHNVGETAQCANDANALFLPHRELTGKVSIVAEHLKNWPVEKQIIAEAKTSDLASVILLASLQNRPIHITGVSNRDDLSLIMTVKEKQDKVTCDINIHALFVSQDDYPEALFLPTIEDQEFFWRNLDAIDAFSIGALPTLLAQITGNEVVTGIGIKEALPLLLSAVNNGRLTIDDIVERLHDNPASIFNIPEQNAEVEIDLDFAFRHTKRWSPYTKGGLRGGVERVLVDGETIVLSGDLVAAEAEGKLVSSSSTKILTPAHPSVEPTPFAGTKKRFSFSNEKRSSFTGFSDDEDSLVDQPLEQRLMSSRPPKELAAPGAIQSLIRGNNPFLRRNILSVNQFKRSDFHALFAVAQELRSAVERDGILDLMKGRLLTTIFYEPSTRTSSSFIAAMERLGGRTVNINTTSSSVKKGETLQDTIRTLACYSDAIVLRHPDEMSAHTAAKYSPVPILNGGNGSREHPTQAFLDLFTIREELGTVNGITVTFMGDLKYGRPVHSLCRLLRHYQVRINLVSPSELRLPQALRTELANAGMLGVESETLTPEIISKSDVLYCTRVQQERFEKREEYEKLKDVYIVDNKILSHAKDQMVVMHPLPRVNEIREEVDYDHRAAYFRQMRYGLFVRMALLAMVMGIDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKT9</accession>
    <name>C5DKT9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pre-mRNA polyadenylation factor FIP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0F07458g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FIP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554527.1">
        <property type="nucleotide sequence ID" value="XM_002554481.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKT9"/>
    <dbReference type="FunCoup" id="C5DKT9">
        <property type="interactions" value="252"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKT9"/>
    <dbReference type="GeneID" id="8292731"/>
    <dbReference type="KEGG" id="lth:KLTH0F07458g"/>
    <dbReference type="eggNOG" id="KOG1049">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039307_2_1_1"/>
    <dbReference type="InParanoid" id="C5DKT9"/>
    <dbReference type="OMA" id="GFNEYTW"/>
    <dbReference type="OrthoDB" id="1917198at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007854">
        <property type="entry name" value="Fip1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051187">
        <property type="entry name" value="Pre-mRNA_3'-end_processing_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13484">
        <property type="entry name" value="FIP1-LIKE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13484:SF0">
        <property type="entry name" value="PRE-MRNA 3'-END-PROCESSING FACTOR FIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05182">
        <property type="entry name" value="Fip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pre-mRNA polyadenylation factor Fip1" evidence="7">
        <location>
            <begin position="165"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="52"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="63"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR24090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="278" mass="29741" checksum="87283566289E873E" modified="2009-07-28" version="1">MNSSDEDERFLYGSDEEGAEAVSPSENKNKRPLTQPNDEEETKKPKVDSTEDNAASSSSSDENSSENEESDEDSDVEFIIGVGADSSKLDSKKDSATAASGPKPTTIAPPATNIGSAAVETEATAGLSTAAVDADAGTSAAPGTERLPAIDLNAVGKLGDQPITDIDPEVLKEKPWRQPGANISDYFNYGFNEQTWMEYLHKQEKLRAEYNPHKMLMGLLALQQQGKLNDSGSDSMGQENAPAAMAPPAFPMGMPPMFGGFPFPFPGMMNNMNPQQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-04-02" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKV4</accession>
    <name>C5DKV4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0F07810p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0F07810g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554542.1">
        <property type="nucleotide sequence ID" value="XM_002554496.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKV4"/>
    <dbReference type="STRING" id="559295.C5DKV4"/>
    <dbReference type="GeneID" id="8292747"/>
    <dbReference type="KEGG" id="lth:KLTH0F07810g"/>
    <dbReference type="eggNOG" id="KOG1618">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030880_3_2_1"/>
    <dbReference type="InParanoid" id="C5DKV4"/>
    <dbReference type="OMA" id="DIMKWNP"/>
    <dbReference type="OrthoDB" id="10251048at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046474">
        <property type="term" value="P:glycerophospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000069">
        <property type="entry name" value="HAD-superfamily subfamily IIA hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050324">
        <property type="entry name" value="CDP-alcohol_PTase-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006357">
        <property type="entry name" value="HAD-SF_hydro_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006353">
        <property type="entry name" value="HAD-SF_hydro_IIA_CECR5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01456">
        <property type="entry name" value="CECR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01460">
        <property type="entry name" value="HAD-SF-IIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14269:SF4">
        <property type="entry name" value="CAT EYE SYNDROME CRITICAL REGION PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14269">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--GLYCEROL-3-PHOSPHATE 3-PHOSPHATIDYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13344">
        <property type="entry name" value="Hydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13242">
        <property type="entry name" value="Hydrolase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="433"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="140"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="565" mass="63112" checksum="1F23E5828BAA8825" modified="2009-07-28" version="1">MSDKITEKLKAFSSKMTDGALGHSNEHAQHAGGDAYNTDSDADVDPLQKSESDRVSQQVTEDQKAHAPGTHIERNADEKPRTKSFLESDGIDEMKPVTRKHRVSSLSLSDQWRANQDLHPLTSMDNNEGTPSSRVPSRKASMVSRHSSVSRRASTVRTRQQIIDHERVASYAFAFDIDGVIVRGPETIPEARDALRMLNGENKYNIKVPYIFITNGGGRSEKARCKDLSKRLNITVTKDQVIQGHTPMKDLVPIYKNVLVVGGVLDSCRKVAQDYGFKNVYIPLDIMKWNPSVTPYYQLSEEENAVARDVDFSKVPIDAILVFADSRNWAADQQIILEILLSKNGVMGTVAKDPSDNKVGLYFAHSDFVWATDYGLSRYGMGALQVSIAALYQEHTGRELQVTRFGKPQRGTFRFAEKVLSNWRRNTLTEHVEQLNLEDGETSDSAPSDSEDEGTDTDGAVLEQTDKLVLDLPPASTVYFVGDTPESDIRFANSHDSSWYSILVKTGVFQGNTTPKYQPKKICENVLEAVKFAIEREHQKELEEWNSSVVDAPVVDERTAKIGNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKY4</accession>
    <name>C5DKY4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">GPN-loop GTPase 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0F08492g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Small GTPase required for proper nuclear import of RNA polymerase II and III (RNAPII and RNAPIII). May act at an RNAP assembly step prior to nuclear import.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Binds to RNA polymerase II (RNAPII).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the GPN-loop GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554572.1">
        <property type="nucleotide sequence ID" value="XM_002554526.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKY4"/>
    <dbReference type="FunCoup" id="C5DKY4">
        <property type="interactions" value="946"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKY4"/>
    <dbReference type="GeneID" id="8292778"/>
    <dbReference type="KEGG" id="lth:KLTH0F08492g"/>
    <dbReference type="eggNOG" id="KOG1534">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037460_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKY4"/>
    <dbReference type="OMA" id="LYTHMTV"/>
    <dbReference type="OrthoDB" id="5839at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17872">
        <property type="entry name" value="GPN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000552">
        <property type="entry name" value="GPN-loop GTPase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004130">
        <property type="entry name" value="Gpn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030228">
        <property type="entry name" value="Gpn3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231:SF7">
        <property type="entry name" value="GPN-LOOP GTPASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231">
        <property type="entry name" value="XPA-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03029">
        <property type="entry name" value="ATP_bind_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR24135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="271" mass="30564" checksum="B38D45ED166CBFCF" modified="2009-07-28" version="1">MSRVGVLVLGPAGAGKSTFCNSIISHMQTVGRRAHIVNLDPAAEASEYEFTVDIRDLISLEDVMEELHLGPNGSLIYCFEYLLNNLDWLEEEIGDYNDEYLIFDCPGQIELYTHIPVLPTIARHLQTQMGFSLCATYLLEAPFVIDSSKFFSGALSAMSAMILLELPHINILSKLDQIKDEYSKKKLKRYLNPDPTLLLDSSNETLNPRFQKLNKTIANLVDDFGMVQFLPLEAKNPESVSNILSYIDDVTQWAEGQEPKEPNDQIELEDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>C5DL30</accession>
    <name>C5DL30_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">L-2-hydroxyglutarate dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="7">1.1.99.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0F09526g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>(S)-2-hydroxyglutarate + A = 2-oxoglutarate + AH2</text>
            <dbReference type="Rhea" id="RHEA:21252"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:16782"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="EC" id="1.1.99.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the L2HGDH family.</text>
    </comment>
    <dbReference type="EC" id="1.1.99.2" evidence="7"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554618.1">
        <property type="nucleotide sequence ID" value="XM_002554572.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DL30"/>
    <dbReference type="STRING" id="559295.C5DL30"/>
    <dbReference type="GeneID" id="8292825"/>
    <dbReference type="KEGG" id="lth:KLTH0F09526g"/>
    <dbReference type="eggNOG" id="KOG2665">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024775_1_0_1"/>
    <dbReference type="InParanoid" id="C5DL30"/>
    <dbReference type="OMA" id="GVHFTRM"/>
    <dbReference type="OrthoDB" id="498204at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047545">
        <property type="term" value="F:(S)-2-hydroxyglutarate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43104">
        <property type="entry name" value="L-2-HYDROXYGLUTARATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43104:SF4">
        <property type="entry name" value="L-2-HYDROXYGLUTARATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="9">
        <location>
            <begin position="37"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00036066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR24181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="409" mass="44891" checksum="B2AF30A1CAD8ECC2" modified="2009-07-28" version="1">MLKFPKRGLLSRQWKAGCTSIRTYTNSLDVATDYSHAVIGGGVVGLSVAHELSKKSENKVLLIEKNPSTGLETSSRNSEVIHAGLYYPVQSLKTKLCLEGSNIIYNELLPLKTGVNWSKCGKWIVAQTDADDAYLESMYLKAKNDLDLPVEMIPANKTKWIEPAIHVGRSVLNSPTSGIIDSHSLMQYLLTGMDNNGVELAIGTELIDMAYDYGRGYTLLCKSTVNNSDETVEIRVENVINAAGLQAHKVANMLLPRNKQKTQYFAKGNYYTLSGSASPPVRRLIYPVPPKSGKSLGTHLTIDLMGQMRFGPDLQYVESPSDYEVSCSNLKAAYEAICRYYPHIQLNDLQPAYSGIRPKLAGPGDKSFKDFYISEEKGLPGFVNMLGIESPGLTAGLAIGRHVCGLYHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLP9</accession>
    <name>C5DLP9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Thiamine pyrophosphokinase</fullName>
            <ecNumber evidence="7">2.7.6.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0G02508g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>thiamine + ATP = thiamine diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11576"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18385"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Cofactor biosynthesis; thiamine diphosphate biosynthesis; thiamine diphosphate from thiamine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the thiamine pyrophosphokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.2" evidence="7"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555147.1">
        <property type="nucleotide sequence ID" value="XM_002555101.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLP9"/>
    <dbReference type="FunCoup" id="C5DLP9">
        <property type="interactions" value="306"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLP9"/>
    <dbReference type="GeneID" id="8293410"/>
    <dbReference type="KEGG" id="lth:KLTH0G02508g"/>
    <dbReference type="eggNOG" id="KOG3153">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044237_0_0_1"/>
    <dbReference type="InParanoid" id="C5DLP9"/>
    <dbReference type="OMA" id="TDMCKAL"/>
    <dbReference type="OrthoDB" id="25149at2759"/>
    <dbReference type="UniPathway" id="UPA00060">
        <property type="reaction ID" value="UER00597"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030975">
        <property type="term" value="F:thiamine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004788">
        <property type="term" value="F:thiamine diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006772">
        <property type="term" value="P:thiamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07995">
        <property type="entry name" value="TPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10240">
        <property type="entry name" value="Thiamin pyrophosphokinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.320">
        <property type="entry name" value="Thiamin pyrophosphokinase, thiamin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006282">
        <property type="entry name" value="Thi_PPkinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016966">
        <property type="entry name" value="Thiamin_pyrophosphokinase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007373">
        <property type="entry name" value="Thiamin_PyroPKinase_B1-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036371">
        <property type="entry name" value="TPK_B1-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007371">
        <property type="entry name" value="TPK_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036759">
        <property type="entry name" value="TPK_catalytic_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13622">
        <property type="entry name" value="THIAMIN PYROPHOSPHOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13622:SF8">
        <property type="entry name" value="THIAMIN PYROPHOSPHOKINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04265">
        <property type="entry name" value="TPK_B1_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04263">
        <property type="entry name" value="TPK_catalytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF031057">
        <property type="entry name" value="Thiamin_pyrophosphokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00983">
        <property type="entry name" value="TPK_B1_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63999">
        <property type="entry name" value="Thiamin pyrophosphokinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63862">
        <property type="entry name" value="Thiamin pyrophosphokinase, substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiamin pyrophosphokinase thiamin-binding" evidence="8">
        <location>
            <begin position="224"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR031057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR24710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="309" mass="35410" checksum="7F29BD7191F02329" modified="2009-07-28" version="1">MESVVENTEELQFQPPAYSHVLDLNGFLSPHQNDSALLILNQKIMVTGVFEKLWRNYFLHVCADGGANRLYEYFDNDEDRSRHIPDYIVGDLDSVREDVLRFYIQKGVAIIRQTSQYATDFTKSLQLISLHFFSSSFHKVTRSSGIDTNYGIEDCHGIHTLYHSERKIWVDRDVDLLVLNAIDGRFDQTIHSITQLYATARTDAYYRLCYLTTTDLIMLVPTGGALIKYSKKFKEECIKNCGLLPVGKPSVIRATRGLKWDVENWPTSIISGKVSSSNRFVGDTCCFFDVEDPMVLNIELNLEKLSMFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLT1</accession>
    <name>C5DLT1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="15">KLTH0G03212p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0G03212g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein sumoylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PIAS family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555179.1">
        <property type="nucleotide sequence ID" value="XM_002555133.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLT1"/>
    <dbReference type="FunCoup" id="C5DLT1">
        <property type="interactions" value="163"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLT1"/>
    <dbReference type="GeneID" id="8293442"/>
    <dbReference type="KEGG" id="lth:KLTH0G03212g"/>
    <dbReference type="eggNOG" id="KOG2169">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014307_0_0_1"/>
    <dbReference type="InParanoid" id="C5DLT1"/>
    <dbReference type="OMA" id="PHPNEIR"/>
    <dbReference type="OrthoDB" id="28127at2759"/>
    <dbReference type="UniPathway" id="UPA00886"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061665">
        <property type="term" value="F:SUMO ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.780">
        <property type="entry name" value="PINIT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.30">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023321">
        <property type="entry name" value="PINIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038654">
        <property type="entry name" value="PINIT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003034">
        <property type="entry name" value="SAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036361">
        <property type="entry name" value="SAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004181">
        <property type="entry name" value="Znf_MIZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782:SF4">
        <property type="entry name" value="TONALLI, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782">
        <property type="entry name" value="ZINC FINGER MIZ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14324">
        <property type="entry name" value="PINIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02037">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02891">
        <property type="entry name" value="zf-MIZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00513">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68906">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51466">
        <property type="entry name" value="PINIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50800">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51044">
        <property type="entry name" value="ZF_SP_RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SAP" evidence="12">
        <location>
            <begin position="19"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="PINIT" evidence="14">
        <location>
            <begin position="119"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="SP-RING-type" evidence="13">
        <location>
            <begin position="305"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="399"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="680"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="820"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="477"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="498"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="534"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="577"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="594"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="624"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="696"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="755"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="783"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="838"/>
            <end position="859"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR24742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="882" mass="97329" checksum="C2B418B3411764E7" modified="2009-07-28" version="1">MTASSGGLHQEIDFCVQEMEKLRVAELKAVSRAIGLSLSGRKADLQDRIRAYLKNSFRLGSIDPWRPKAILVLIDKAKLSDPLPTYESVWQSLRTGAFRHPVATGHQPPSALNPSAIEVHKPPPVPRAPKTRLALQFGESPFYKLRRMLNGSPKLAAKNNGRGVCIYRFLLNQEESSILGKGPQFKLYLFSGILDPLENRGDAPIQFPHPNEIRFNDVQVKDNVRGLKNKPGTAKPADLTPYLRPSGSENVLQLIYAFTKSDYLMYCYLVEVISPEKILQEVLRHPKIVKPATLQYLKETLSEDEDEDLVTTSTVMTLQCPISYCRMRYPSKSVYCQHLQCFDSLWFIESQQQIPTWHCPVCQKKIKIEDLALCEFVEEIIQQCDEEVEQVEISRDGTWKPVFEDDPPAPQQHSATEVKLENGHSLEGIVRPGSEDSDEEPISSRRRNLPQNEPVVISLDSDDEEEEAPKAAPQPPGENQTPSQPGQELQERPGGRTAPSNSTLPHENTLANIYGTYMDINSGHEPQRSLDPKNANTTPSSARFNFNHGTPNNSETPNSSPFIQPHHQIPNLLGKTPLNNNVNESYTSGHEQGQKSPSPTVSVQQSSSRVHPSFGEVNVPLLNAHNQGPNIQSGHTPVRRHSENTSAYGQEVRLTYRPSSFANNTLPPPWQDVRGQQSLLGLGGDTSQIHREARRSSTGSYNGENSLEGVQDHSRSPPLVARQPVINGAPIHNSPHSERGATSSAPPFSKPPAPAALASTGPLISSPRSQGQPPPLPPVPQSINRAESGQKPASASNARYRKPIVSPFIPKKPYVNMLPQKRQISSTSINKVAPTGHNAHSSQTRQPQLENEGVSVSATSPPPLLAQSMANELDFIDLTSDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLW4</accession>
    <name>C5DLW4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0G04048p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0G04048g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555212.1">
        <property type="nucleotide sequence ID" value="XM_002555166.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLW4"/>
    <dbReference type="FunCoup" id="C5DLW4">
        <property type="interactions" value="30"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLW4"/>
    <dbReference type="GeneID" id="8293478"/>
    <dbReference type="KEGG" id="lth:KLTH0G04048g"/>
    <dbReference type="eggNOG" id="ENOG502RZDT">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117800_1_0_1"/>
    <dbReference type="InParanoid" id="C5DLW4"/>
    <dbReference type="OMA" id="EMISCAT"/>
    <dbReference type="OrthoDB" id="4085867at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014841">
        <property type="entry name" value="Rad33"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08730">
        <property type="entry name" value="Rad33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR24775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="176" mass="20096" checksum="50CBA81CB3911841" modified="2009-07-28" version="1">MNRTKSKAYNSVSEFLNPKIPPEIEDDILGAFANYSIESDMTAQDLPEYFKELQMPTEMTKLVQPQDLCIEGTKVVDFDKLLKTTFHLLIFMNNAEIIDTQWLMLVQGCGRDTQFPQVTLRNHVLSIKDLQKIANSINVDGGHLIDMMSCATQGKRVYITWMDFAYLLGKLGHLVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>C5DM25</accession>
    <name>C5DM25_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">Vacuolar protein sorting-associated protein 29</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0G05390g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the VPS29 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555273.1">
        <property type="nucleotide sequence ID" value="XM_002555227.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DM25"/>
    <dbReference type="FunCoup" id="C5DM25">
        <property type="interactions" value="1113"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DM25"/>
    <dbReference type="GeneID" id="8293539"/>
    <dbReference type="KEGG" id="lth:KLTH0G05390g"/>
    <dbReference type="eggNOG" id="KOG3325">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063749_0_0_1"/>
    <dbReference type="InParanoid" id="C5DM25"/>
    <dbReference type="OMA" id="IGCCNGY"/>
    <dbReference type="OrthoDB" id="10258130at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024654">
        <property type="entry name" value="Calcineurin-like_PHP_lpxH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000979">
        <property type="entry name" value="Phosphodiesterase_MJ0936/Vps29"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00040">
        <property type="entry name" value="yfcE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11124">
        <property type="entry name" value="VACUOLAR SORTING PROTEIN VPS29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12850">
        <property type="entry name" value="Metallophos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="5">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="148"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="156"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="192"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR24836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="256" mass="28097" checksum="4A58DB770BD91CEB" modified="2009-07-28" version="1">MLLLALSDAHIPERAIKDLPQKFKKLISVPNKIQQVVLLGNCTKSYSFLKFVNQISSNVVVVRGEFDNKTVAREEIPLNTIIKQGDFTIGCCNGYTVVPKSDPLSLLTLARQLDVDIVLWGGTHNVEAYTLEDKFFINPGTCTGAFNPDWPLPDASNDSTLEASADSSEPQTQASEGSTDNANDATVPAQDGKSDQDDVANKEDEDTDSEIDDFEVDGRCIPSFCLLDIQGSTCTLYIYTYVDGEVKVDKVVYNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DML4</accession>
    <name>C5DML4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0G09878p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0G09878g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555462.1">
        <property type="nucleotide sequence ID" value="XM_002555416.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DML4"/>
    <dbReference type="FunCoup" id="C5DML4">
        <property type="interactions" value="91"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DML4"/>
    <dbReference type="GeneID" id="8293740"/>
    <dbReference type="KEGG" id="lth:KLTH0G09878g"/>
    <dbReference type="eggNOG" id="KOG0234">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006383_3_2_1"/>
    <dbReference type="InParanoid" id="C5DML4"/>
    <dbReference type="OMA" id="KPYGITW"/>
    <dbReference type="OrthoDB" id="267323at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003873">
        <property type="term" value="F:6-phosphofructo-2-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006003">
        <property type="term" value="P:fructose 2,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006000">
        <property type="term" value="P:fructose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1240:FF:000025">
        <property type="entry name" value="6-phosphofructo-2-kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001101">
        <property type="entry name" value="6-phosphofructo-2-kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003094">
        <property type="entry name" value="6Pfruct_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013079">
        <property type="entry name" value="6Phosfructo_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606:SF32">
        <property type="entry name" value="6-PHOSPHOFRUCTO-2-KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606">
        <property type="entry name" value="6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01591">
        <property type="entry name" value="6PF2K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00991">
        <property type="entry name" value="6PFRUCTKNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="6-phosphofructo-2-kinase" evidence="4">
        <location>
            <begin position="144"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="671"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="715"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="7"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="23"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="74"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="136"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="671"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="725"/>
            <end position="737"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR25025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="737" mass="82992" checksum="594DBA76DBE4EA28" modified="2009-07-28" version="1">MFKPVQFSDSTTAPSSDSEVEPLESHQSEHQKHVRLDVGSENSPPISPNAPENGLPEFQKRPLSDTPVGSYWNSPSDSQETSSDSTPIPSPHGSSSNLRQFETALGEDGESKTSNNSHFIHPKVRPTTIDVPGLTKSRTSPDGTISNKDPGSKLVIVMVGLPATGKSFITNKLSRFLNYSMYYCKVFNVGNTRRQFAKEHNLQDQDSNFFDPNNPESKKLREKWAMDTLDQLLDYLLDGPGSVGIFDATNSTKNRRKKVLEKIYSRNNQIKVLYLESVCSDKTTVEKNIQLKLFGPDYKGKDPEASLQDFKERLSNYVRAYEPVEDDEDLQYIKMIDVGKKVISYNIQGFLASQTVYYLLNFNLAERQIWITRNGESEYNVQGKIGGDSDLTLRGKRYAQALSRFIDKQRISFTEHEMKAHMESNPDGKNFSATEFFVWTSMLNRAVQTGNYFNEDDYPIKQMRMLDELSAGDCEGLTYAEIQAKHPDEFEERLNDKLRYRYPGIGGESYMDVINRLRPVITEIERIKENLLIVTHRVVARVLLGYFLNLSRDIIANLDVPLHCVYCLEPKPYGVTWSLYEYDESTDDFFKVPHTELNTMKVKEVGLVHSERRYSIVPTAPPSVANTKGEFLANNASSTSLAKTALPRLPVVGGGTSIARPGRRPQLISATSSRFARNNSNNKDGASERSISTPPLTGGRKEIELNKLNEKLAQLNASESKENTGSKPVMSRSQSEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMT0</accession>
    <name>C5DMT0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0G11374p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0G11374g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Endomembrane system</location>
            <topology evidence="6">Peripheral membrane protein</topology>
            <orientation evidence="6">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the VPS18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555528.1">
        <property type="nucleotide sequence ID" value="XM_002555482.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMT0"/>
    <dbReference type="FunCoup" id="C5DMT0">
        <property type="interactions" value="936"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMT0"/>
    <dbReference type="GeneID" id="8293807"/>
    <dbReference type="KEGG" id="lth:KLTH0G11374g"/>
    <dbReference type="eggNOG" id="KOG2034">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003488_0_0_1"/>
    <dbReference type="InParanoid" id="C5DMT0"/>
    <dbReference type="OMA" id="KFFVFPC"/>
    <dbReference type="OrthoDB" id="1845386at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098588">
        <property type="term" value="C:bounding membrane of organelle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030897">
        <property type="term" value="C:HOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048284">
        <property type="term" value="P:organelle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007033">
        <property type="term" value="P:vacuole organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006904">
        <property type="term" value="P:vesicle docking involved in exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16462">
        <property type="entry name" value="RING-H2_Pep3p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000547">
        <property type="entry name" value="Clathrin_H-chain/VPS_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007810">
        <property type="entry name" value="Pep3/Vps18_beta-prop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058919">
        <property type="entry name" value="Pep3/Vps18_RING_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23323">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23323:SF26">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 18 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05131">
        <property type="entry name" value="Pep3_Vps18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26148">
        <property type="entry name" value="VPS18_RING_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50236">
        <property type="entry name" value="CHCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Pep3/Vps18 beta-propeller" evidence="8">
        <location>
            <begin position="27"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="588"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="domain" description="Pep3/Vps18 RING C-terminal" evidence="9">
        <location>
            <begin position="835"/>
            <end position="918"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF05131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF26148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR25091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="936" mass="107005" checksum="51C2CE4BAE416491" modified="2009-07-28" version="1">MCIEILQFLKYLEKHLTKNTLKGWAAMKVAIEHVQLQFIKEIENNVCSLQVESNFMCFALKTGHMFLIDLESPSNVIKYHFALLSSPQEKLLRVWMDPSATTLLLKTNFAKYYVLTVDSLGKGNESVTPLKKLSKKNRDVISVMWQGKNNFVCGTTEGKVYVVNTQSENSATKAYSSPHSIDGLIFASSPGAEKCAFLATGHTLRYWSNPKDPLTTLKELPPTETEQFEEIEQTSSRKFVFFQDTFAWTIDSGTIFGTVKGSGDVLKSATVLLNVELPPSNHRVKGVALSKYHLLLLRGNEILIINKLNNEVVAQETIWNQGSEKFLELVVDYSQSPPTFWCYSTSNIYEIVLEGENNGIWRLLSKLGKYDEALKLPGLAPAEKDFILEQKGDYLFSEEEFTEAARCYGVSDSVTTAEVALKLMKKSDLVALQAFLTAKLNTARQRKNSIVQITLLSDWVVWNFMQMLNDVDELISSEQDDQNLESWRLKKESLGKELMAFFKKNLEILDRDTVYQIMARQNRKLEVLSFARLINDYKYVLSYWIRSKNWYEALKVLALTQDLECVYKYATILLINSPDSTVNTWMQIKGVNPSELISSLLTYFSNFQKMHSSADSSRSIPNYALKYLKWCIQEPEDAENLAPIVFNSAIFMMIAGFDAKGRENEIIDFIEAHSGCFDSDFVLRLSIKFKRYKTCIYVYSWLRLYEEAVTLAIKMDLLEDAKLVASTPELDGNSKLRRKLWLMIAQCMIQQQNDTKQTIREILQDSNGILGIKDLLPLFGEFTTIANVKDELIKSLEKHNSTMARVSHEIENSIKIKREIVEDIETLKARFQTLEPGASCDCCDRVLQTRKFYVFPCGHSFHTDCLVREILKSTDHALRSKIETIQRSATKGMTLDNLKELDRLLSTKCCLCSDIKINSIDEPLESDEKERHAWEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>C5DN00</accession>
    <name>C5DN00_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">3beta-hydroxysteroid 3-dehydrogenase</fullName>
            <ecNumber evidence="8">1.1.1.270</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0G13046g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="6">Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 5/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the short-chain dehydrogenases/reductases (SDR) family. ERG27 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.270" evidence="8"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555598.1">
        <property type="nucleotide sequence ID" value="XM_002555552.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DN00"/>
    <dbReference type="FunCoup" id="C5DN00">
        <property type="interactions" value="169"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DN00"/>
    <dbReference type="GeneID" id="8293881"/>
    <dbReference type="KEGG" id="lth:KLTH0G13046g"/>
    <dbReference type="eggNOG" id="KOG1478">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029944_1_0_1"/>
    <dbReference type="InParanoid" id="C5DN00"/>
    <dbReference type="OMA" id="WHNIDGY"/>
    <dbReference type="OrthoDB" id="9989144at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000253">
        <property type="term" value="F:3-beta-hydroxysteroid 3-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000525">
        <property type="entry name" value="3-keto-steroid reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051593">
        <property type="entry name" value="Ergosterol_Biosynth_ERG27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43647:SF1">
        <property type="entry name" value="3-KETO-STEROID REDUCTASE ERG27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43647">
        <property type="entry name" value="DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0752">Steroid biosynthesis</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR25161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="347" mass="39507" checksum="D7AC73F11B572975" modified="2009-07-28" version="1">MDVDRKVAVITGTNSNLGLNTAYRLIQELDQDVRLTIVVTSRTLPRAREVIDNIKDFVGKSSRPGLVDYDYLLVDFTDMVSTLNAYYELNKHYKEINYFFVNAAQGVYSGIDWLGAVKEVFTNPIEAVTNPTYKIQRVGVKSQDGMGLVFQANVFGPYYLIQKILPQLQAGKAVVVWISSIMSDPKYLSLEDIELVKSPASYEGSKRLVDLLHLATYKELKGQGIHQYVVQPGIFISHSFFKYLNVLSYYGMLLLFYFARFLGSPWHNIDGYRAANAPVYVATLANPTFEQQNVKYGSATYKDGMEYIRTQEIEATGVHDVYVYLKKLKDVWDDKLKDQITNSRQKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DN36</accession>
    <name>C5DN36_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0G13838p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0G13838g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555634.1">
        <property type="nucleotide sequence ID" value="XM_002555588.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DN36"/>
    <dbReference type="FunCoup" id="C5DN36">
        <property type="interactions" value="91"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DN36"/>
    <dbReference type="GeneID" id="8293917"/>
    <dbReference type="KEGG" id="lth:KLTH0G13838g"/>
    <dbReference type="eggNOG" id="ENOG502QQZD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005863_0_0_1"/>
    <dbReference type="InParanoid" id="C5DN36"/>
    <dbReference type="OMA" id="NMYTPGC"/>
    <dbReference type="OrthoDB" id="2503993at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005621">
        <property type="term" value="C:cellular bud scar"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902929">
        <property type="term" value="C:plasma membrane of growing cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000282">
        <property type="term" value="P:cellular bud site selection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011043">
        <property type="entry name" value="Gal_Oxase/kelch_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024982">
        <property type="entry name" value="Rax2-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048266">
        <property type="entry name" value="Rax2-like_second"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048265">
        <property type="entry name" value="Rax2-like_third"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31778">
        <property type="entry name" value="BUD SITE SELECTION PROTEIN RAX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31778:SF2">
        <property type="entry name" value="BUD SITE SELECTION PROTEIN RAX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12768">
        <property type="entry name" value="Rax2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20842">
        <property type="entry name" value="Rax2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20843">
        <property type="entry name" value="Rax2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50965">
        <property type="entry name" value="Galactose oxidase, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950550" evidence="3">
        <location>
            <begin position="21"/>
            <end position="1214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1155"/>
            <end position="1183"/>
        </location>
    </feature>
    <feature type="domain" description="Rax2-like second" evidence="5">
        <location>
            <begin position="248"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="Rax2-like third" evidence="6">
        <location>
            <begin position="423"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="domain" description="Rax2-like C-terminal" evidence="4">
        <location>
            <begin position="880"/>
            <end position="1111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1125"/>
            <end position="1145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1125"/>
            <end position="1143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF20842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF20843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR25197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1214" mass="132418" checksum="6DB3733255D0B417" modified="2009-07-28" version="1">MRATTCLATSVLALAKFCSGSHLDALESKLGVEVYDIPNLDLSKSKNNEVQLLGNFKDINILKYSGQKNFTGEVSGNPENALIYYSNETFLEIYSSSDSSEVFSVDHIVPFKDDAFILSGAGSISGHQLSRQLLFNLSSLEFREIFGQDLAQVNTITANGDKVFFGGSFEYEMSNQTIHSLLVWDSIKDGVEVLPFGGFGKNSTVNSILNLDNSNILFAGNFSAVDNTQQLNNINVSNTSNTSVPELGHQISLQSAAWVSDGTLDKSAIKCPSTESTGWLKTGSTQGQFELYMPEETRPSKLRVFNARDSDEQVSLFRIVTTPSNGIMNLTYLDPTTGELASCDAWCPLLSSQNLTDISSKSYPGDQVQYFENRTTLQWSESFQDFAFVNNVPVSDLTFIALDSYGSSVGLAGIELYESAYSVYANNTLNAPNCAQGSATSNALLSNSSAWDSGSSEGNYLSTSVQDSETNPFVIFYPSIIYPGVYTIDVLTPGCLEDSSCQTRGIINATIRDVQNNTLLDSIEIYQNNDYEKFDTLYSGFLDSEVKVTLEFDGAINSGTEVPVMVASKIVVNIEDFDQSVFENNTSGFINGLLHYSTSNTSSFLSELGSYSATDLAHYSVSKLPKHSNIFANMFEDDLVLFTTDGEMARLKLANNLSIEEYTFETIGKDISTISEYSGGLIIVGTFNGSSEPAARGFNGTFFNLTEFNSTVRTFSNLTLGQTEMLIFDKNIILNGTTGEKIYDTSKLTLEVASAGRNPYNDTLFQGYVLQNDYTNLNGSFFISTNSEEVSQNFWALGGKLPYDAAFVDNSTTAYAYYNPDNTSNSFGVSVMNDSGSTVQLSAWSGKVGAMASSKNDSLLAIGGKDQHTDSQLLVTNSSSGESLASYKWDSEISLNSIIFFARNNSVLVGGSFRVNNTQCSGLCLFNYQDKKWSPFLNNSIRGVINRMEIFNNSNLLIAGSFRLNETDGVSLASICLKDSSSEILHEGNITLNGFVSMDGSSNNVVAFSDTSLLQLESGKWQQISTNFTENSKFKGLDVFPLKDTTRKQKRDSSSKVLLITGSLQHAKFGSINAAFYDSGEWTPFLSSNQATSSSDLETSHVFLNRDLSDFLDYKGYLQGTFSSNTSSGTSGTPSPSGSSPSNLQRKKKKIDRGFIVLISLALALGTLAVLGLLGVIFSYFFGDSGDGYQLSKPRTDENEMIDTVPPEKLMRFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>C5DN69</accession>
    <name>C5DN69_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0G14608p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0G14608g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555667.1">
        <property type="nucleotide sequence ID" value="XM_002555621.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DN69"/>
    <dbReference type="FunCoup" id="C5DN69">
        <property type="interactions" value="41"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DN69"/>
    <dbReference type="GeneID" id="8293952"/>
    <dbReference type="KEGG" id="lth:KLTH0G14608g"/>
    <dbReference type="eggNOG" id="ENOG502QSRA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016933_0_0_1"/>
    <dbReference type="InParanoid" id="C5DN69"/>
    <dbReference type="OMA" id="IRYWNEF"/>
    <dbReference type="OrthoDB" id="5588846at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033254">
        <property type="term" value="C:vacuolar transporter chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016237">
        <property type="term" value="P:microautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006799">
        <property type="term" value="P:polyphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14474">
        <property type="entry name" value="SPX_YDR089W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051572">
        <property type="entry name" value="VTC_Complex_Subunit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46140">
        <property type="entry name" value="VACUOLAR TRANSPORTER CHAPERONE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46140:SF1">
        <property type="entry name" value="VACUOLAR TRANSPORTER CHAPERONE COMPLEX SUBUNIT 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03105">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="729"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="765"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="799"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="domain" description="SPX" evidence="8">
        <location>
            <begin position="1"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="568"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="570"/>
            <end position="586"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR25230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="825" mass="92250" checksum="93A27D28015EFC1E" modified="2009-07-28" version="1">MKFGSQIHDRSVPEWRLNNIDYKHLKEVVKKATTLRPDKEPAASLEEDKELQGLYRAFQTQCRSVNAFTSLKIKETSTRLVSVESSILRLQQRADLPSQKRKRQLALIGSHLDHCNMELLRICRFLILQKIALRKLLKKFLKYYPYGKETAEQFVKRLKSSPELTEGEDGISFVKVDLDPYLLEVSLVVDVLQELEQGETGSSKGLVREGSSGETPGKFRGVDFSSKSIPTDTDLTFDSAFLGKATRLQSFLVSGEDVSQVKFLLLQMGFHVVDDDMLIASQKSVKNSSPANLNNLASSQGPRAVKSFHDLQVALNQHQNAASPKISDIKSSQNIDITLLDSDPMPIILANEAANQHPTMIVTGFKASKCIIMCHVGGLRNHIVSDNVPVGTLSNALPGKELEDTSSSEADTTALSSIRNACLEWIRSHDMKPVGPLISTKRTRFTKNIEKGESQTYLVCIDEDIALDRKHKVPYAFVEIRRLPSGPDTSQGKDQDEEVVTGLIEKLLETKSSTFPLRKDQTLWKLLYLAHSAQDIEAALIETVNPSLTHFSNEALFEAGSKELSDVTAAKRRKSGTISSHRKRDVLRKDKSSSPDPQPKPRIRYWNEFDDGDEAYDGYQSFGSNDEESQVRGSVDNGFIVFNKNFINTMYSFSERFKDFISFSGRSSERRPLLADSLRGPSLNSVTTSSTSYTGVSAERDYDRYLDFTKQQTDSDSLYETKHDLVITFFYLSSLLISCITSGISLGIVASIFRELNDDILLGPGPGLVTIIIVTLLVSLLLSCTSLLLLFSRFQMAPWWHYVGCFLIFLVVACTVCYGLIEIFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNC8</accession>
    <name>C5DNC8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0G15928p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0G15928g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIN1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555726.1">
        <property type="nucleotide sequence ID" value="XM_002555680.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNC8"/>
    <dbReference type="FunCoup" id="C5DNC8">
        <property type="interactions" value="289"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNC8"/>
    <dbReference type="GeneID" id="8294012"/>
    <dbReference type="KEGG" id="lth:KLTH0G15928g"/>
    <dbReference type="eggNOG" id="KOG3739">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008055_0_0_1"/>
    <dbReference type="InParanoid" id="C5DNC8"/>
    <dbReference type="OMA" id="HWHENVK"/>
    <dbReference type="OrthoDB" id="241990at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031932">
        <property type="term" value="C:TORC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038203">
        <property type="term" value="P:TORC2 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031567">
        <property type="entry name" value="CRIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008828">
        <property type="entry name" value="Sin1/Avo1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031313">
        <property type="entry name" value="Sin1_PH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056385">
        <property type="entry name" value="UBL_AVO1/Sin1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13335">
        <property type="entry name" value="TARGET OF RAPAMYCIN COMPLEX 2 SUBUNIT MAPKAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13335:SF1">
        <property type="entry name" value="TARGET OF RAPAMYCIN COMPLEX 2 SUBUNIT MAPKAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16978">
        <property type="entry name" value="CRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16979">
        <property type="entry name" value="SIN1_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23164">
        <property type="entry name" value="UBL_AVO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRIM" evidence="3">
        <location>
            <begin position="551"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="domain" description="AVO1/Sin1 ubiquitin-like" evidence="5">
        <location>
            <begin position="749"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="domain" description="SIN1-type PH" evidence="4">
        <location>
            <begin position="959"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="81"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="225"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="287"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="336"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="439"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="487"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="84"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="129"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="160"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="171"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="225"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="248"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="338"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="445"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="462"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="495"/>
            <end position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR25289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1069" mass="118968" checksum="22BAF65F8576C58C" modified="2009-07-28" version="1">MDVVKSINRLRASFITNSDDEDQARRVIQPFTNNVETALPASIEDIYRTHSGENLLTVLESPPISRDYMDRITSLQKTRFDRTSAVTGSTKTKALTRDSSRDLDLSIKNSDKETTVSATTSRAEPANTLKPTTSRGTSVGNNSLKKSGPALPKSATPKAEFGDSRGSETKVKRKKLLSKIFKGHGHDNGSRSSAIQETKPTDRNKNKAGTSFYDSAFYYDEILEEDDDDDDDDDDDDAERNDEFFLSGLSTSPENGSGKPNKTSKLANFPRYAQNVLSAPDIKSNAIHTHNEGKSSSSLHNRDEIRSTPSRAEASDLDSYMNEDDLKGLDLESDKLSDLQTDEPTNRAQSSARSGESGTATSTSDESNYDFSDKSSYGRSLLGSDLQKIENTGYAREDDAGSSAVDDSILADDLMHLDSMPGHSVPQSQTFKLLSVGQPSTFMNDRPRIRPDDERLRGDGNNFPNRNTTTGSASSKRNVIAHSLTGSITKAKPSPQGSSNETCQGSQVRVTSRHRRTASEPKIAGRMPRSSAEPLVFNKVELNKPGEGCSSQLTNMLNKKKSQASNPLSYFGFVSGDKVTTSESTTLHAYIQDSREFKKKPFILNVRKSASVFEVIGFLLYQYQTKKKPEADVDSSNSGELENPNDYVLKIVDEDGEPFEDNFGTIDRKKKIGALFDNEVVLCRAKNEEEKERNELDTPLPYCQETDCTGEEGLVSKSVTEPGIKQLSYYKPILNVTENPADTEEDVLADVKIFLYPNVNPEFNYTKIKAPLSTSLNDVLLRYCKMKSMNPLDYALKISGKRLIADLESSVGELDGNYKLELITKKEIKALGLKSMKKSTLGKPTLPTIQSADLTPVTLDNRERYLAAVSSINPQITDTNDGDSKKSGLIPVKKAAHSRHKRNKILQNGESNAGPAGFFKLKNSSKSSLRNSNQAYQPSFSDNSGLNLDTSYKDIFAGAYYKYRVWRRQQMSFINKHERTLAIDGDYIYLIPPEDGYNWHHESTKTKCFHMSQVVFIERSKRIPEYFKMFVNRPSGLKRYYFEAVNAAECTELVTRIQKLVSAYKMNHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNL8</accession>
    <name>C5DNL8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">KLTH0G18194p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0G18194g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555816.1">
        <property type="nucleotide sequence ID" value="XM_002555770.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNL8"/>
    <dbReference type="FunCoup" id="C5DNL8">
        <property type="interactions" value="315"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNL8"/>
    <dbReference type="GeneID" id="8294111"/>
    <dbReference type="KEGG" id="lth:KLTH0G18194g"/>
    <dbReference type="eggNOG" id="KOG0583">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051040_0_0_1"/>
    <dbReference type="InParanoid" id="C5DNL8"/>
    <dbReference type="OMA" id="FYANIDY"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="2"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="362"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="362"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="134"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="84"/>
            <end position="86"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="138"/>
            <end position="139"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="8">
        <location>
            <position position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR25379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="415" mass="45650" checksum="BB42BFCC1034E63A" modified="2009-07-28" version="1">MYSRLAKVQTGSFSTVYRAYDTERGQEVALKIIEKPADAELARKLTRLVGSERDILRALGTQHPNVCAMLDFYERETCYVFVMEYAARGDLYDVIRGWRKGGAAGREQVDLARIVVQLCSAIQYAHVRGIAHRDIKPENVLLDSAGNVKLADWGLSCRARVSQDTRIGTEKYLAPEAYSCAHDTFLADFWSLGVTLLFVMFGACPFKNACLTKTPNNPNFAHFLANPHRFISEYYFRPLHERGAGTKGSPQLRRDSVGVEGPAEWLALPQALLPTERERLLLLCATCVVTCLLVINPRARSMQEFLLRMASLLPRGESKSHAVPQQQQQRQSPDKLDTDFFYSGLVTDPDAVASIYGAGSCSGSSTSDAATSSDLAESTGSEQSSQSAQSTQSLPSAPSARPAQPAKILSEELEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>C5E1W8</accession>
    <name>C5E1W8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein FMP52, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Protein fmp52, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0H00264g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FMP52 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555891.1">
        <property type="nucleotide sequence ID" value="XM_002555845.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E1W8"/>
    <dbReference type="FunCoup" id="C5E1W8">
        <property type="interactions" value="110"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E1W8"/>
    <dbReference type="GeneID" id="8294202"/>
    <dbReference type="KEGG" id="lth:KLTH0H00264g"/>
    <dbReference type="eggNOG" id="KOG4039">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071330_2_2_1"/>
    <dbReference type="InParanoid" id="C5E1W8"/>
    <dbReference type="OMA" id="CIENAKA"/>
    <dbReference type="OrthoDB" id="430436at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051170">
        <property type="term" value="P:import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000366">
        <property type="entry name" value="Protein FMP52, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014843">
        <property type="entry name" value="Him1/Fmp52"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14097">
        <property type="entry name" value="OXIDOREDUCTASE HTATIP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14097:SF7">
        <property type="entry name" value="OXIDOREDUCTASE HTATIP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08732">
        <property type="entry name" value="HIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00071738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00074024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR30029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="222" mass="24040" checksum="9EE20506B816788F" modified="2009-07-28" version="1">MSSRALIIGATGLCGSAFVKYVAKDPSFTEIATISRKALNGNYDGKVRSLILEDSSNWSAAVPENYDILFSGLATTRGEAGKENFYKVDHDMNLQLARSAKEKGYSTYVVVSSTGADENSMFYYMKTKGELERDILALGFERTIILRPGPLLGERTKSKGLLNGLSSKIGSIVYRTKLQSVFGCPVYGDEVAKVGVKLALDTSDTAKIKIVESAEILRLAGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5E1Y7</accession>
    <name>C5E1Y7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0H00704p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0H00704g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555910.1">
        <property type="nucleotide sequence ID" value="XM_002555864.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E1Y7"/>
    <dbReference type="FunCoup" id="C5E1Y7">
        <property type="interactions" value="1152"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E1Y7"/>
    <dbReference type="GeneID" id="8294222"/>
    <dbReference type="KEGG" id="lth:KLTH0H00704g"/>
    <dbReference type="eggNOG" id="KOG1155">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018320_2_1_1"/>
    <dbReference type="InParanoid" id="C5E1Y7"/>
    <dbReference type="OMA" id="ERCLYHS"/>
    <dbReference type="OrthoDB" id="10262026at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045842">
        <property type="term" value="P:positive regulation of mitotic metaphase/anaphase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000926">
        <property type="entry name" value="Anaphase-promoting complex subunit CDC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007192">
        <property type="entry name" value="APC8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558">
        <property type="entry name" value="CELL DIVISION CYCLE 16,23,27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558:SF10">
        <property type="entry name" value="CELL DIVISION CYCLE PROTEIN 23 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04049">
        <property type="entry name" value="ANAPC8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0498">Mitosis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 7" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Cdc23" evidence="8">
        <location>
            <begin position="12"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="425"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="493"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR30048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="621" mass="72333" checksum="CF303A6E079D8966" modified="2009-07-28" version="1">MLESSLMNFLSELKLELRESAVQLSRLKLYNSSKWSAEALCGMCEIPSVPVMKTFMKNDDSPLRNRSNIQFEQQESPQKHLSGMESSEYDLYLLASTLFECKEYDRCAYYLADSKHPELKFLKLYSKYLSWDKKTQENMESSLAPRSSKQNTGFRENAEIANREIMNRLHSHESGNSNALKKSQKSVDLDNGESTSIPMILKELNNYLLLEGEKPTFGISLLHYLRGVLYKIQDIPSNAISSFLSSLTLFPFNWVCWTELLSCISRTDESVLLLKILSEKFTDEKTQVMLKFFKLSIFQEFSGNIDDFIQELDYLLSIFPNFAFLKTQHALINYHYMDYVSAGLIFEQIIKLDPYRLEDMDTYSNILYVMQKPSKLAYLAQFASGVDRFRAETCCIIANYYSAKQEHEKSILYFRRALTLNKNCTSAWTLMGHEFVELKNSHAAIECYRRAVDINPRDFKAWYGLGQAYEVLDMHLYSLYYFQKSCALKPLDKRMWQALASCYEKVDNLEESIKCYTRALQLSLDSDIDTTILFRLAVLYEKQKDIISCKEYMLRCVEAQHSTDGFINDETTKASLWLARYEARYKNFSEAYKHALRVTHGSSQEIEEARAIVRECRKRMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5E208</accession>
    <name>C5E208_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ubiquinone biosynthesis protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0H01232g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Membrane-associated protein that warps the membrane surface to access and bind aromatic isoprenes with high specificity, including ubiquinone (CoQ) isoprene intermediates and presents them directly to Coq7, therefore facilitating the Coq7-mediated hydroxylase step. Participates in the biosynthesis of coenzyme Q, also named ubiquinone, an essential lipid-soluble electron transporter for aerobic cellular respiration.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the COQ9 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555931.1">
        <property type="nucleotide sequence ID" value="XM_002555885.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E208"/>
    <dbReference type="FunCoup" id="C5E208">
        <property type="interactions" value="268"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E208"/>
    <dbReference type="GeneID" id="8294244"/>
    <dbReference type="KEGG" id="lth:KLTH0H01232g"/>
    <dbReference type="eggNOG" id="KOG2969">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057411_1_1_1"/>
    <dbReference type="InParanoid" id="C5E208"/>
    <dbReference type="OMA" id="SELFMAQ"/>
    <dbReference type="OrthoDB" id="619536at2759"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013718">
        <property type="entry name" value="COQ9_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012762">
        <property type="entry name" value="Ubiq_biosynth_COQ9"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02396">
        <property type="entry name" value="diverge_rpsU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21427">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS PROTEIN COQ9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21427:SF19">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS PROTEIN COQ9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08511">
        <property type="entry name" value="COQ9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="7 8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 8" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="domain" description="COQ9 C-terminal" evidence="9">
        <location>
            <begin position="157"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR30069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="257" mass="28814" checksum="D4EE2483AAE1ADA1" modified="2009-07-28" version="1">MLALSRILARRLYHPSALEHQLAHSFAPLTYGPGSTQFKVLEHALNKHVPSYGFNERALVASLNDLDLGPSLLSVIGASNSPSFLNASPAVLELIKFHLVTKRYALTKELDPARTSSPAEPPALETLFHRRLELNKPIAPHLTQLLSSLSIPGEFLVQTALPELHRLSDDMVYFSKEPDANDFAWYSKRIALSCAFVSSELFMAQDRSANYADTFEFAAEKLHNVSKLGQYYNNTEEYMWYTLLMSVNLAKSQLTRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>C5E236</accession>
    <name>C5E236_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0H01848p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0H01848g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555959.1">
        <property type="nucleotide sequence ID" value="XM_002555913.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E236"/>
    <dbReference type="FunCoup" id="C5E236">
        <property type="interactions" value="21"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E236"/>
    <dbReference type="GeneID" id="8294272"/>
    <dbReference type="KEGG" id="lth:KLTH0H01848g"/>
    <dbReference type="eggNOG" id="KOG4035">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017272_2_1_1"/>
    <dbReference type="InParanoid" id="C5E236"/>
    <dbReference type="OMA" id="ISLRHTY"/>
    <dbReference type="OrthoDB" id="445362at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071933">
        <property type="term" value="F:Arp2/3 complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000147">
        <property type="term" value="P:actin cortical patch assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051666">
        <property type="term" value="P:actin cortical patch localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030125">
        <property type="entry name" value="SPIN90/Ldb17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018556">
        <property type="entry name" value="SPIN90/Ldb17_LRD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13357:SF1">
        <property type="entry name" value="NCK-INTERACTING PROTEIN WITH SH3 DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13357">
        <property type="entry name" value="SH3 ADAPTER PROTEIN SPIN90 NCK INTERACTING PROTEIN WITH SH3 DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09431">
        <property type="entry name" value="SPIN90_LRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SPIN90/Ldb17 leucine-rich" evidence="2">
        <location>
            <begin position="223"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="449"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR30097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="483" mass="55572" checksum="61179E6797691225" modified="2009-07-28" version="1">MERGSSSPDDEEPNAAYSPSIFWEEMEHLLKVPDVTDETQVNTALVKYIKHGSDSYQKCVKTEQGLYRLGVTFTESTMFHRNKMFVVSKLLSLLSVDLLECNLKFIISYIILCASKSDLSVLELLVEFQGFTVVYNNLYSTFAYLNRYGEDIAPSSSSGPRSDAGTEIESEIIESLKQVSTILMDILFQIFKYSKCDIVNLSIVDDFFVYYLMRTMRSETTEDIFNNAKFKLLLALNEQYMIFSYQYELENRVYKYLVDSTVSKSFVELLLLKFNRVTDRSLQIMLCKIVYLILTMDESYAKDFFYLNDLHVLVDVLIRELNNIADDEELIRNTVLRVLFPLLRNTELAQTQYRRSDLIDILQHLSSLDNVCSSGETKDEHRTTVRLAQKCLKQIDWLQVPVVDTSDQTVPVYSDDSREMSVSTGSLSSPYDFQEKHLYGNYDASLSAESITKRLQPPPPPPARKHPHRLGSGQKIAQFRPKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2B5</accession>
    <name>C5E2B5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Coatomer subunit beta'</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">KLTH0H03608g</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12 13">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Cytoplasmic vesicle</location>
            <location evidence="1 13">COPI-coated vesicle membrane</location>
            <topology evidence="1 13">Peripheral membrane protein</topology>
            <orientation evidence="1 13">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Golgi apparatus membrane</location>
            <topology evidence="13">Peripheral membrane protein</topology>
            <orientation evidence="13">Cytoplasmic side</orientation>
        </subcellularLocation>
        <text evidence="13">The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the WD repeat COPB2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556038.1">
        <property type="nucleotide sequence ID" value="XM_002555992.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2B5"/>
    <dbReference type="FunCoup" id="C5E2B5">
        <property type="interactions" value="1438"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2B5"/>
    <dbReference type="GeneID" id="8294351"/>
    <dbReference type="KEGG" id="lth:KLTH0H03608g"/>
    <dbReference type="eggNOG" id="KOG0276">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005507_1_0_1"/>
    <dbReference type="InParanoid" id="C5E2B5"/>
    <dbReference type="OMA" id="YVDYYPQ"/>
    <dbReference type="OrthoDB" id="10261470at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22947">
        <property type="entry name" value="Coatomer_WDAD_beta-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000016">
        <property type="entry name" value="Coatomer alpha subunit, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.470:FF:000001">
        <property type="entry name" value="Coatomer subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006692">
        <property type="entry name" value="Beta-prop_COPA/B_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050844">
        <property type="entry name" value="Coatomer_complex_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016453">
        <property type="entry name" value="COPB2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056176">
        <property type="entry name" value="TPR_COPA_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19876">
        <property type="entry name" value="COATOMER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19876:SF2">
        <property type="entry name" value="COATOMER SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04053">
        <property type="entry name" value="B-prop_COPA_B_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23953">
        <property type="entry name" value="TPR_COPA_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005567">
        <property type="entry name" value="Coatomer_beta'_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 13" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7 13" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="9 13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 13" id="KW-0813">Transport</keyword>
    <keyword evidence="5 14" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="9"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="93"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="136"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="197"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="224"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="COPA/B second beta-propeller" evidence="16">
        <location>
            <begin position="327"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="domain" description="COPA/B TPR" evidence="17">
        <location>
            <begin position="608"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="815"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="840"/>
            <end position="850"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR005567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF04053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF23953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAR30176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="875" mass="98024" checksum="1C5EAC976798DE67" modified="2009-07-28" version="1">MKLDIKKAFSNRSDRVKGIDFHPTEPWVLTTLYSGRVEIWNFETQTEVKSITVTETPVRAGKFIARKNWIVVGSDDFKIRVFNYNTGEKIADFEAHPDYIRSIAVHPTRPYVLSASDDLTMKFWNWEKNWSLEQTFEGHEHFVMGVAFNPKDPSTFASACLDHTVKIWSLGQPTANFTLHAHETRGVNYVDYYPLQDKPYLITSSDDRTVKIWDYQTKSCVATLEGHMANVSFAVFHPALPIIISGSEDGTLKVWNSNTYKLEKTLNLGLERSWCIATHPTGKRNYVASGFDNGFTVLSFGNDDPKLSLDPVGKLVWCGGKNASATDVFTAAIRGTEGADDGEPLPLQTKELGNVDVFPQSLKHSPNGRFVTVVGDGEYIVYTALAWRNKAFGKCHDFVWGPDSNSYALIDESGHVKFHKNFKELTSWSIPLEFGVEKLYAGALLGVKADGFVYFFDWDSGSLVRRIDVDANDVVWSENGELLMIINNVDENNTEERAAYVLGFNREAYESFLASEEEPSEDGIDEAFDVLYEVSDPIGSGKWVGDVFIFTTKTNRLNYFVGGKSYNLAHFDKEMYMLGYLPRDNKVYLADRDIHIYSYEISIEVLEFQTLVLRGELEQAKTSVLPNIEGKENLLKISRFLEGQEYHEDALEISPDNDQKFDLALKLSKLSLAHDIVAGDDNEFKWRKLGDKALEGFDFQLAIEAYDNAADLESLFLLYSSFKDQAGLVDVGDRAQRLGKYNLAFNAYWSAGDVDRARDLLVSSGRYSEAAVLSKAYGGKLEDTNAIVAQWKESLIEVGKAGVASRIHELSKADQVSAEEPLIDIETEAGQGEKQPVGEPLEEAATDETPTDGTPTDETPPETHAEDEAAQNLQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2F8</accession>
    <name>C5E2F8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0H04598p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0H04598g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556081.1">
        <property type="nucleotide sequence ID" value="XM_002556035.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2F8"/>
    <dbReference type="STRING" id="559295.C5E2F8"/>
    <dbReference type="GeneID" id="8294394"/>
    <dbReference type="KEGG" id="lth:KLTH0H04598g"/>
    <dbReference type="eggNOG" id="ENOG502S1E3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038609_0_0_1"/>
    <dbReference type="InParanoid" id="C5E2F8"/>
    <dbReference type="OMA" id="KDKHRAN"/>
    <dbReference type="OrthoDB" id="273070at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019496">
        <property type="entry name" value="NUFIP1_cons_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10453">
        <property type="entry name" value="NUFIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMR1-interacting protein 1 conserved" evidence="2">
        <location>
            <begin position="164"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="287"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="290"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR30219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="329" mass="37604" checksum="3CD10435E6946B8E" modified="2009-07-28" version="1">MNYNHGSNYNGGDQYYNRPNIPKPTSSGQLPNQHPHSNQRADANFQPHQTSAYFQGHQTFGATQGYGQNYYGSYPQYYYPAQQAPYYAGGYQGYIANYASPAEPPKEAVKNNSAERKSVISYDDTDTETNIISQDQNGGSKEKRHSEVGTPDDPKETIEAENKEAKIIAIPGTSITLESEEDIKKWREERRKMWLVKISNQREKHKSDLGIEEEDVSRNEAFQQVKRDKQFIQSIQNQINRSGPSPNLDLKLIQRTMAKENIKLLNFIKELGDAGLLQYELKEEEKQKLFGSNSRQPGARTNFNNKRPNTPHEGPSRYEGSKKRQHKPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2J5</accession>
    <name>C5E2J5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0H05500p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0H05500g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M20A family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556118.1">
        <property type="nucleotide sequence ID" value="XM_002556072.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2J5"/>
    <dbReference type="STRING" id="559295.C5E2J5"/>
    <dbReference type="GeneID" id="8294433"/>
    <dbReference type="KEGG" id="lth:KLTH0H05500g"/>
    <dbReference type="eggNOG" id="KOG2275">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021802_11_0_1"/>
    <dbReference type="InParanoid" id="C5E2J5"/>
    <dbReference type="OMA" id="TIDSWTH"/>
    <dbReference type="OrthoDB" id="3064516at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000328">
        <property type="term" value="C:fungal-type vacuole lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05674">
        <property type="entry name" value="M20_yscS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000027">
        <property type="entry name" value="N-fatty-acyl-amino acid synthase/hydrolase PM20D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001261">
        <property type="entry name" value="ArgE/DapE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017141">
        <property type="entry name" value="Pept_M20_carboxypep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047177">
        <property type="entry name" value="Pept_M20A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45962">
        <property type="entry name" value="N-FATTY-ACYL-AMINO ACID SYNTHASE/HYDROLASE PM20D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45962:SF1">
        <property type="entry name" value="N-FATTY-ACYL-AMINO ACID SYNTHASE_HYDROLASE PM20D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037217">
        <property type="entry name" value="Carboxypeptidase_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00758">
        <property type="entry name" value="ARGE_DAPE_CPG2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 7" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="9">
        <location>
            <begin position="279"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="163"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="232"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="541"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR037217-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR037217-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR30256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="573" mass="63181" checksum="75DD147C3423DB5F" modified="2009-07-28" version="1">MSDYLPIGGSKPRGIFERRRLLATVVLGACALLSVILLAGHTVNSPNNKDSICSECAGFKALRPAFNKSLNHILYNQDYKMSVIEKLAGAVKIPTEIFDFTPSPSDDPEAWVHFQELHEYLETQFPYVYEKAQVEKIDGFGLLFTWQGSKPDLKPLLLMAHQDVVPVEPNTVDMWTHPPFSGFYNSSEDLIYGRGTADIKQLVVSHLEAVERLMLDGFKPQRTIMISFGCDEEASGSCAAKIAQHIEKRYGANSIYAILDEGGQVGEVDGVYFAEPVTSEKGYLDAEITVFTPGGHSSVPPDHTSIGILGELIVDLEKDPYDILIEENNPALETIKCFIKFGAIKDEEIAKHLSKNNLKKLGTALDNRLALKYLFKTSQALDIVSGGIKANALPESATVLVNNRIGMHSSVNETLGHIMKHVQSKAETYNLGIKVLAGRYSKEETAEVIKPVTESGRFVIRLLDPLEPAPFSPTSGSQVWDIISGTTVSTYKQKLFGGEDAKVYITPALSTGNTDTKSFWNLTRNIYRYSGSLQYKESNEHTVDEKNSGLSLLSGVAFMYQYIPNVDAYATDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2L6</accession>
    <name>C5E2L6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Actin-related protein 2/3 complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0H05962g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
            <location evidence="7">Cytoskeleton</location>
            <location evidence="7">Actin patch</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the WD repeat ARPC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556139.1">
        <property type="nucleotide sequence ID" value="XM_002556093.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2L6"/>
    <dbReference type="FunCoup" id="C5E2L6">
        <property type="interactions" value="840"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2L6"/>
    <dbReference type="GeneID" id="8294454"/>
    <dbReference type="KEGG" id="lth:KLTH0H05962g"/>
    <dbReference type="eggNOG" id="KOG1523">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034396_1_0_1"/>
    <dbReference type="InParanoid" id="C5E2L6"/>
    <dbReference type="OMA" id="YVWEPSP"/>
    <dbReference type="OrthoDB" id="406844at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005885">
        <property type="term" value="C:Arp2/3 protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000682">
        <property type="entry name" value="Actin-related protein 2/3 complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017383">
        <property type="entry name" value="ARPC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10709">
        <property type="entry name" value="ACTIN-RELATED PROTEIN 2/3 COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10709:SF2">
        <property type="entry name" value="ACTIN-RELATED PROTEIN 2_3 COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038093">
        <property type="entry name" value="ARP2/3_su1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR038093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR30277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="373" mass="40612" checksum="9452063CB5A0E492" modified="2009-07-28" version="1">MSNSIVANLKLAKAPIYSHCFSQDRSLVAVTLNSDCLVFKLTPGGKPALVATLPDHDKTVTAVDISIHGRIVTCSQDRNAIVWEPLSDGTYKPTLVLLRINRAATCVQWAPSGYKFAVGSSARIIAVCYYEQENDWWVSKHIKKPIKSTINCVSWHDNGIMLACGGTDGYVRAFSGFVKGLDSRDQVANSPWAEKFPFGALIREWYHGAWIHDVQWRSSQEKLAYVAHDGSFNVVDPSDHVSTTFSPEGLPLMALVWINDHEALCGGFSCHPVLFGEGASGWQFSKNIDKDIAPSTPASASTGDDDDEGNAPFGMSALKKFKELDLKGKVSAQVQESTHENAIVELAPYSVTAGNVTEVSSCGLDGKIVIYRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2W9</accession>
    <name>C5E2W9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Acetyl-CoA hydrolase</fullName>
            <ecNumber evidence="4">3.1.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Acetyl-CoA deacylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0H08338g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetyl-CoA + H2O = acetate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20289"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="3.1.2.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the acetyl-CoA hydrolase/transferase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.1" evidence="4"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556242.1">
        <property type="nucleotide sequence ID" value="XM_002556196.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2W9"/>
    <dbReference type="FunCoup" id="C5E2W9">
        <property type="interactions" value="241"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2W9"/>
    <dbReference type="GeneID" id="8294560"/>
    <dbReference type="KEGG" id="lth:KLTH0H08338g"/>
    <dbReference type="eggNOG" id="KOG2828">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019748_3_0_1"/>
    <dbReference type="InParanoid" id="C5E2W9"/>
    <dbReference type="OMA" id="SCIVPMV"/>
    <dbReference type="OrthoDB" id="10250396at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008775">
        <property type="term" value="F:acetate CoA-transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003986">
        <property type="term" value="F:acetyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006083">
        <property type="term" value="P:acetate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.750.70:FF:000002">
        <property type="entry name" value="Acetyl-CoA hydrolase Ach1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1080.10:FF:000003">
        <property type="entry name" value="Acetyl-coA hydrolase Ach1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1080.20:FF:000001">
        <property type="entry name" value="Acetyl-CoA hydrolase Ach1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.70">
        <property type="entry name" value="4-hydroxybutyrate coenzyme like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1080.20">
        <property type="entry name" value="Acetyl-CoA hydrolase/transferase C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1080.10">
        <property type="entry name" value="Glutaconate Coenzyme A-transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026888">
        <property type="entry name" value="AcetylCoA_hyd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038460">
        <property type="entry name" value="AcetylCoA_hyd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046433">
        <property type="entry name" value="ActCoA_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003702">
        <property type="entry name" value="ActCoA_hydro_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43609">
        <property type="entry name" value="ACETYL-COA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43609:SF1">
        <property type="entry name" value="ACETYL-COA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13336">
        <property type="entry name" value="AcetylCoA_hyd_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02550">
        <property type="entry name" value="AcetylCoA_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acetyl-CoA hydrolase/transferase N-terminal" evidence="9">
        <location>
            <begin position="12"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="Acetyl-CoA hydrolase/transferase C-terminal" evidence="10">
        <location>
            <begin position="332"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR30380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="524" mass="57827" checksum="C08A18669544E713" modified="2009-07-28" version="1">MTISSVLKKRVRYAPYLERLVQPQELVPLFRTGQNLGWSGFTGVGAPKIVPQVLADHVATNSLQGQLRFNLFVGASAGPEESRWAELDMISRRSPHQVGKPISRAINDGRIQFFDKHLSMFPQDLTYGFYTRERPAGAPILDYAIIEATAIKEDGSIVPGPSVGASPEFVAVADKVILEVNTATPSFEGMHDIDMPVNPPHRPPYPYMSVDQRNGLDSIPIDPAKVVAIVESNQRDIVPPNTEADDASRAIASHLIGFLEHEVTCGRLPANLHPLQSGIGNIANAVIEGLSSASFKNLTVWTEVLQDSFLDLFENGSLDFATATSIRLTEPGFRRFFDNWDEFSKKLCLRSQVVSNNPELIRRLGVIAMNTPVEVDIYAHANSTNVSGSRMLNGLGGSADFLRNAKLSIMHTPAARPSKTDPTGISTIVPMASHVDQTEHDLDIVVTDQGLADLRGLSPTERAREIINHCAHPDYKPILTDYLDRAEFYARKHKCLHEPHILKNAHKFHINLAEKGTMKVDSWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2X8</accession>
    <name>C5E2X8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0H08668p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0H08668g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATPase g subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556251.1">
        <property type="nucleotide sequence ID" value="XM_002556205.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2X8"/>
    <dbReference type="FunCoup" id="C5E2X8">
        <property type="interactions" value="121"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2X8"/>
    <dbReference type="GeneID" id="8294575"/>
    <dbReference type="KEGG" id="lth:KLTH0H08668g"/>
    <dbReference type="eggNOG" id="KOG4103">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083674_1_0_1"/>
    <dbReference type="InParanoid" id="C5E2X8"/>
    <dbReference type="OMA" id="CAQAYLN"/>
    <dbReference type="OrthoDB" id="437at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015078">
        <property type="term" value="F:proton transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015986">
        <property type="term" value="P:proton motive force-driven ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006808">
        <property type="entry name" value="ATP_synth_F0_gsu_mt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04718">
        <property type="entry name" value="ATP-synt_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="4" id="KW-0138">CF(0)</keyword>
    <keyword evidence="5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR30389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="119" mass="13291" checksum="E337BD44E51A32C8" modified="2009-07-28" version="1">MLARIQSFATGIASKAQTYTTKTFYYSKVVGELSKQVYLKEGLQPPAISDFQSVYTKLYKQALDLALKPRQSLSLFKNIQKDSVLKYGAYGVQLAGLYSLGEVIGRRKVVGYTNYAAHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>C5E320</accession>
    <name>C5E320_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0H09614p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0H09614g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556293.1">
        <property type="nucleotide sequence ID" value="XM_002556247.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E320"/>
    <dbReference type="FunCoup" id="C5E320">
        <property type="interactions" value="40"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E320"/>
    <dbReference type="GeneID" id="8294618"/>
    <dbReference type="KEGG" id="lth:KLTH0H09614g"/>
    <dbReference type="eggNOG" id="ENOG502S3JK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_141194_0_0_1"/>
    <dbReference type="InParanoid" id="C5E320"/>
    <dbReference type="OMA" id="MTEQISH"/>
    <dbReference type="OrthoDB" id="203678at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08700">
        <property type="entry name" value="VPS51_Exo84_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR30431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="133" mass="15214" checksum="B8C1026829D9C0FE" modified="2009-07-28" version="1">MAEQITHKRPIRIKIDKDKRKQLQDFYKLREEQSPVSDNINENHESVDQDVASASEDCVEPPTLKISESTLSELVSAHNTLLGKETEVNNTIKNTIYENYYDLIKVNELLKSVGNDSSDRLAQLSDILKLLEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5E361</accession>
    <name>C5E361_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0H10648p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0H10648g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556334.1">
        <property type="nucleotide sequence ID" value="XM_002556288.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E361"/>
    <dbReference type="FunCoup" id="C5E361">
        <property type="interactions" value="454"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E361"/>
    <dbReference type="GeneID" id="8294665"/>
    <dbReference type="KEGG" id="lth:KLTH0H10648g"/>
    <dbReference type="eggNOG" id="KOG0257">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_4_0_1"/>
    <dbReference type="InParanoid" id="C5E361"/>
    <dbReference type="OMA" id="PRDFKLC"/>
    <dbReference type="OrthoDB" id="2414662at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016212">
        <property type="term" value="F:kynurenine-oxoglutarate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000024">
        <property type="entry name" value="Kynurenine--oxoglutarate transaminase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051326">
        <property type="entry name" value="Kynurenine-oxoglutarate_AT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43807">
        <property type="entry name" value="FI04487P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43807:SF20">
        <property type="entry name" value="FI04487P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="6">
        <location>
            <begin position="54"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR30472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="443" mass="49821" checksum="07FB53F076E6061B" modified="2009-07-28" version="1">MIKHLSRKLSMSVGKQRQIVPNLALTQNLTKDVWSLTNEAAAAAAHNAQNKGRDLINLGQGFFSYSPPAFVVEEAQKALDDAMVNQYSPTKGRMSLINSLIKQYSPLYNTQLQANNIQVTTGANEGILACLVGLLNPGDEVIVFEPFFDQYISNIQIPGGKVTYVPLHPPKEMSVRPTEGTEWTVDYDELRAAITPKTKALILNTPHNPVGKVFTREELLQIGQICVENNVVIISDEVYEHLYFTPEFTRVATLSPEIGQITLTIGSAGKSFAATGWRIGWIVSLNPELLEYASMAHTRICFASPSPLQEACANALPIALDNGYFKKMREEYIHKFNIFTRVFDELGLPYTKPEGTYFVLVDFSKVKIPEDYPFPEELRSKAKDFRISYWLINELGVVAIPPTEFYIKEHEKVAENLLRFAVCKTDDYLEGAVERLRLLKEHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5E387</accession>
    <name>C5E387_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ribonuclease P protein subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0H11242g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic/archaeal RNase P protein component 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556360.1">
        <property type="nucleotide sequence ID" value="XM_002556314.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E387"/>
    <dbReference type="FunCoup" id="C5E387">
        <property type="interactions" value="249"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E387"/>
    <dbReference type="GeneID" id="8294691"/>
    <dbReference type="KEGG" id="lth:KLTH0H11242g"/>
    <dbReference type="eggNOG" id="KOG4046">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_998037_0_0_1"/>
    <dbReference type="InParanoid" id="C5E387"/>
    <dbReference type="OMA" id="NMIGIEG"/>
    <dbReference type="OrthoDB" id="124041at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030677">
        <property type="term" value="C:ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033204">
        <property type="term" value="F:ribonuclease P RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.210">
        <property type="entry name" value="Ribonuclease P/MRP, subunit p29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016848">
        <property type="entry name" value="RNase_P/MRP_Rpp29-subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036980">
        <property type="entry name" value="RNase_P/MRP_Rpp29_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023534">
        <property type="entry name" value="Rof/RNase_P-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002730">
        <property type="entry name" value="Rpp29/RNP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13348:SF0">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13348">
        <property type="entry name" value="RIBONUCLEASE P SUBUNIT P29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01868">
        <property type="entry name" value="RNase_P-MRP_p29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF027081">
        <property type="entry name" value="RNase_P/MRP_p29_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00538">
        <property type="entry name" value="POP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101744">
        <property type="entry name" value="Rof/RNase P subunit-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR027081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR30498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="274" mass="31280" checksum="8CA5E9DFE4608C84" modified="2009-07-28" version="1">MNREQTFITEQILAKSLPTAEKAVDENRLQDTLLLLPTDGGLSSRLKKKNGKLRLLSGEHIKNSTHSAYREINKNSKHALKSYINGARKASSTAKRIASEKNLRERPELLGYLKEKHPSVFGSIPRFDQILPMHEELWVNYMREILNIPDDVPSASKLNINGTNALLKLSMADYNGCMITVTKSRNENLRGIKGIVIWDSQKDFILLCRGELVDEIKCVPKMGTIFAFEIPVNKDEALQYTILGDRFKYRSSDRAGRKFKSRRCDDMLYYISGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3A9</accession>
    <name>C5E3A9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0H11792p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0H11792g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556382.1">
        <property type="nucleotide sequence ID" value="XM_002556336.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3A9"/>
    <dbReference type="GeneID" id="8294716"/>
    <dbReference type="KEGG" id="lth:KLTH0H11792g"/>
    <dbReference type="eggNOG" id="ENOG502S51I">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_159429_0_0_1"/>
    <dbReference type="InParanoid" id="C5E3A9"/>
    <dbReference type="OMA" id="KHPCSKS"/>
    <dbReference type="OrthoDB" id="3969899at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR30520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="122" mass="13740" checksum="01047A4DF22F4550" modified="2009-07-28" version="1">MLNRVFANSSNSLLRALARSGSRFVHSSAPALLYKKWGDISLKDKQAFINNYVGLYKEKHPCSKSNVMYQTLVGEMEEYDDAPYVFGILYNEIRSVSQNESADNAKGSGAMGDPDFEKLLYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3B3</accession>
    <name>C5E3B3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Lysophospholipase</fullName>
            <ecNumber evidence="6">3.1.1.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0H11924g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 1-acyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15177"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.5"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the lysophospholipase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.5" evidence="6"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556386.1">
        <property type="nucleotide sequence ID" value="XM_002556340.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3B3"/>
    <dbReference type="FunCoup" id="C5E3B3">
        <property type="interactions" value="35"/>
    </dbReference>
    <dbReference type="GeneID" id="8294722"/>
    <dbReference type="KEGG" id="lth:KLTH0H11924g"/>
    <dbReference type="eggNOG" id="KOG1325">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014602_2_0_1"/>
    <dbReference type="InParanoid" id="C5E3B3"/>
    <dbReference type="OMA" id="FIANCRN"/>
    <dbReference type="OrthoDB" id="4084751at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046475">
        <property type="term" value="P:glycerophospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002642">
        <property type="entry name" value="LysoPLipase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728">
        <property type="entry name" value="CYTOSOLIC PHOSPHOLIPASE A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728:SF56">
        <property type="entry name" value="MEIOTIC PHOSPHOLIPASE SPO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01735">
        <property type="entry name" value="PLA2_B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00022">
        <property type="entry name" value="PLAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51210">
        <property type="entry name" value="PLA2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 5 6" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="4 5 6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PLA2c" evidence="7">
        <location>
            <begin position="1"/>
            <end position="618"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR30524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="618" mass="68919" checksum="DA61C1FF8E603476" modified="2009-07-28" version="1">MRGKLEVFMRSLNVRAADVENLVNISHQQINMGLAVSGGGYRSMLTGSGFLLGMQEYGLVDCLNYITGVSGGSWILAKLISNGFDFDSLGQWELERSLLEGVPDLDIPDGNVATMLETNNLSEIFKADKWFYDKLTQASGLWKRNADPQPLDIFEQFYSELEQYTQLQPLGRLESRNPKPLSRITKAVARLFSSGGGKEEQVNKLLNASNEFRQVVQTYVALHLKVKSKKFEGFPLSFTDYWGHALIGAIAPSSEAKSVSSLLDQSPAFQNFKTPLPIFVANCKNKDLENAVFEFTPFEFGSWNSLGLFVKLRFLGSKIVAGQALKCFTGFDEVGFLAATSSSLFNNVLVYVWHLVAPSMDMRMAVRAIFSAFNLHGIKAEIEASNGGKEFSHPEYAIYQPNPFFGYPGKNNILAEQDQLYLVDGGEDGENIPIGPLLIEQRRLDVIFALDSSSDMGSFPNGSMLRNFYENHEIKSSSSSGPFNLLPYIPPSGEFVNEGLLSRPVAFGCHLESFPELYGRNQGNLSAVPLPPIIIYHANSEHSFPSNMSTFKLRYTADEVQKMIQNGKDIFNFDSSPKYKRCLACIISKRSYDRATIDSEEAPLPFFCEVCFSEYCYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3C6</accession>
    <name>C5E3C6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Putative mitochondrial carrier protein PET8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0H12232g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556399.1">
        <property type="nucleotide sequence ID" value="XM_002556353.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3C6"/>
    <dbReference type="FunCoup" id="C5E3C6">
        <property type="interactions" value="785"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3C6"/>
    <dbReference type="GeneID" id="8294736"/>
    <dbReference type="KEGG" id="lth:KLTH0H12232g"/>
    <dbReference type="eggNOG" id="KOG0768">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015166_3_0_1"/>
    <dbReference type="InParanoid" id="C5E3C6"/>
    <dbReference type="OMA" id="IGPRTMW"/>
    <dbReference type="OrthoDB" id="276989at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000018">
        <property type="entry name" value="S-adenosylmethionine mitochondrial carrier protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45667">
        <property type="entry name" value="S-ADENOSYLMETHIONINE MITOCHONDRIAL CARRIER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="4"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="96"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="193"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00073559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR30537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="274" mass="29869" checksum="DAF5B2158219B50C" modified="2009-07-28" version="1">MDRASFISSLLAGAAAGTSTDLVFFPIDTLKTRLQAAGGFFANGGYHGVYRGLGSAVVASAPSASLFFVSYDSMKTYSRPVFSKLISSSDQMAETATHMFSSSAGEIAACMVRVPAEVIKQRTQTHKSDSSWQTFKKLLKNDNGEGIRRNLYRGWSTTIMREIPFTCIQFPLYEYLKKRWAQVGNREQVAPWQGSFCGCLAGGVAAATTTPLDVLKTRLMLSKTSVPVLHLARTIYAKEGWQVFFSGVGPRTIWISAGGAIFLGVYETIHSILS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3J4</accession>
    <name>C5E3J4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">KLTH0H14058p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0H14058g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein sumoylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NSE2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556467.1">
        <property type="nucleotide sequence ID" value="XM_002556421.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3J4"/>
    <dbReference type="FunCoup" id="C5E3J4">
        <property type="interactions" value="62"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3J4"/>
    <dbReference type="GeneID" id="8294829"/>
    <dbReference type="KEGG" id="lth:KLTH0H14058g"/>
    <dbReference type="eggNOG" id="KOG2979">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088986_0_0_1"/>
    <dbReference type="InParanoid" id="C5E3J4"/>
    <dbReference type="OMA" id="IELICPI"/>
    <dbReference type="OrthoDB" id="756301at2759"/>
    <dbReference type="UniPathway" id="UPA00886"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030915">
        <property type="term" value="C:Smc5-Smc6 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061665">
        <property type="term" value="F:SUMO ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16651">
        <property type="entry name" value="SPL-RING_NSE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1010">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054753">
        <property type="entry name" value="MMS21_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026846">
        <property type="entry name" value="Nse2(Mms21)"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004181">
        <property type="entry name" value="Znf_MIZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21330">
        <property type="entry name" value="E3 SUMO-PROTEIN LIGASE NSE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21330:SF1">
        <property type="entry name" value="E3 SUMO-PROTEIN LIGASE NSE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22326">
        <property type="entry name" value="MMS21_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11789">
        <property type="entry name" value="zf-Nse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51044">
        <property type="entry name" value="ZF_SP_RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SP-RING-type" evidence="11">
        <location>
            <begin position="188"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR30605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="286" mass="32312" checksum="82D89BA5561800F9" modified="2009-07-28" version="1">MSLLKFQAGKRIAFTTNTNTKPMPRETPTAVPESVPLHHTALQVFHDWRPSDISAILNETRSQFSQLLDQLSEVNATSPDVVDTLTQTFREFRHLEAQQNSLQNNIDLAKSKYRSQCEELPPVTWETWDAYREGELRAPHLLHVFEETERDSESQPPPANSADELSRLYRALPHIAADPQCILPDEAADDDVQIAGGQIELACPITCKPFQRPMISRKCGHVFDHAGIEQYLSAQSTRDCPQGACGQKVSMRDFVPDRLMELRCKITRAREGSRKAQRALENVVPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3L0</accession>
    <name>C5E3L0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0H14432p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0H14432g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556483.1">
        <property type="nucleotide sequence ID" value="XM_002556437.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3L0"/>
    <dbReference type="FunCoup" id="C5E3L0">
        <property type="interactions" value="359"/>
    </dbReference>
    <dbReference type="GeneID" id="8294845"/>
    <dbReference type="KEGG" id="lth:KLTH0H14432g"/>
    <dbReference type="eggNOG" id="KOG0475">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003181_2_2_1"/>
    <dbReference type="InParanoid" id="C5E3L0"/>
    <dbReference type="OMA" id="MFLKINM"/>
    <dbReference type="OrthoDB" id="44789at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005247">
        <property type="term" value="F:voltage-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006878">
        <property type="term" value="P:intracellular copper ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03684">
        <property type="entry name" value="ClC_3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3080.10">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014743">
        <property type="entry name" value="Cl-channel_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001807">
        <property type="entry name" value="ClC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45711:SF9">
        <property type="entry name" value="ANION_PROTON EXCHANGE TRANSPORTER GEF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45711">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00654">
        <property type="entry name" value="Voltage_CLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00762">
        <property type="entry name" value="CLCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81340">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0868">Chloride</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="78"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="157"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="260"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="339"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="379"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="436"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="464"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="497"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="529"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="9">
        <location>
            <begin position="677"/>
            <end position="725"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR30621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="761" mass="83965" checksum="3FF60D2952274BE8" modified="2009-07-28" version="1">MSFQPLDVDDLGNSGPEEVPETKNFAQFQTVDWIQESTPLRRAGERQANEAGNATDAGAPVARWRRYRDLAWRRCQEIITLTCIAVLLGLVAGSIQIVTETLVNWKSGHCARNWLLNKSFCCASVTSTGRGPLSRRDELQCIEGGTWIDRTNPFLDFSLFLLLSVLFATASTLMVQYLAPMATGSGISEIKVWVSGFKYKDEFLNLTTLLVKSTALPLAISSGLSVGKEGPSVHYATCCGFVVTNLLLKRGTRFAEQAEYLIASAAGGVAIAFGAPIGGVLFALEEISTSSNFNLSILWKCYYVALGAVATLQYMDPFRNGKIVMFEVTYDNQWKFQEIPIFLILGAFGGAYGLLISAWNVRFVNFRKTYLQDWRTQEVIFLASITALVSYFNEFLRLDMAESMGILFHECVAGDGESLRTHRICQLDTQTHAGSFLQLLAALLIATVVRSLLIVVSYGCSVPAGIFVPSMAVGATFGRAISLLVEKWITGPAVITPGTYAFLGAAATLSGITNLTLTVVVIMVELTGAFSYIIPLMIAVAVTHLIYSFFSSKGGIADQMIIFNGFPLMENTKQDIDCLEGCSAEDIMTCRVVSVPETLPLFQLKAIVSDADHLKQLLVIDSETHACLGYVNTSILSSELTHAQDSQLPKSTPVNFIESKDWQWENETEAINLCHLVNQSPMSVSARTPLRLLHQIFYKLGCKLAVVESQGSVQGLITKKDFLEFQRLKHRQLHGPLYSFDLKWEQKLWETIKTVFKVTSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBG0</accession>
    <name>C5DBG0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0A02310p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0A02310g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551559.1">
        <property type="nucleotide sequence ID" value="XM_002551513.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBG0"/>
    <dbReference type="FunCoup" id="C5DBG0">
        <property type="interactions" value="40"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBG0"/>
    <dbReference type="GeneID" id="8290356"/>
    <dbReference type="KEGG" id="lth:KLTH0A02310g"/>
    <dbReference type="eggNOG" id="ENOG502QVB0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_457135_0_0_1"/>
    <dbReference type="InParanoid" id="C5DBG0"/>
    <dbReference type="OMA" id="TWTTCYN"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47941">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13812">
        <property type="entry name" value="PPR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="219"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR21117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="597" mass="68823" checksum="3750902E56DD4EAF" modified="2009-07-28" version="1">MGLLATLGSGIAKNGIREPSVVAEKSFRAVPTKARCGVDLKVDRQGGVQPTKLKNEYVLRNIHVVGKGSNFERSAVQDYLSPFTSHQFARHKLPCAYNEDRARANFTALKKLKSSKNSETLLFSSSQQYVGEMIPLLVALTPQEVSTGHAKRNFRSEVFEEIPSIIDFTQNAESFANYVTLLTHSKFYYKKSSFLNGVIPKILRNILHPSNMKTMQFRDVGVFNDVIFFFSEKSDYATCRELFSQMKLEGVKPNTKTFNLMLRNALKNSHIRKSRHPLHDAVYYLRQMQHHEIKADAVTWVTCFNLLLEDMSRDVFLENMIKSNVPITPQLVLAVLSSNPLNSSQALKFLSEYSVPLNSKLFNFCIKKLLSEEKYEAAWAFVDHAHKNADFNLDHESLNAFLRRFAESGRLDLALLTFNTACKRYQISGNLHSFDMLFKALVRNGYTQNFPIVFEYLSRKRRRYARGVQIFSYWLSKAHSMVKFNMKHQVTEGDIEKAKSLLDSALWTSKGLRWKCWRESEQSQRKVFRYLGCIPTTVKAKTTHFIHDTSPEASAKKVKYKNRIRFLAIQNAMAKRIPYAHDRYRALKEELRHRGIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBV3</accession>
    <name>C5DBV3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0A05610p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0A05610g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551702.1">
        <property type="nucleotide sequence ID" value="XM_002551656.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBV3"/>
    <dbReference type="GeneID" id="8290506"/>
    <dbReference type="KEGG" id="lth:KLTH0A05610g"/>
    <dbReference type="eggNOG" id="ENOG502SFPM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_742000_0_0_1"/>
    <dbReference type="InParanoid" id="C5DBV3"/>
    <dbReference type="OrthoDB" id="4036494at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR21260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="373" mass="41589" checksum="69B4F5D548EC13D1" modified="2009-07-28" version="1">MGILYDHEEWAVYAEGKQLLIWREGTLVNEWTVQGIQDYQFYTAEDELVIIGNWGQGLTRLRIKEGTEELSEESALRTSFHIAQFQILPSSGVILAYDKVAGVLHLFNSREERYQGFIKAHPAQTSFSSHGPSEFQVFVRDFDPASLVIKTYLNRYRLRGSNASLINTIPMEGLFIKRWVVRSACGAFFAVISTSECEQACQLKFFLQDASIPFHTETLEGHAVSAAFVSPLKLFLILRLAQGNALCLLNLESSHTHQLLSLEAEFIRSSHEIDEAGNFTQPSSVPAITHLDSNLKLVNSSSNSIFFVMNSAPNILFCWSFTKNIDSHLDTWAFPAKVVSLGELNSATVAAVCLTENPTSLSKIEISQPFSGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBW1</accession>
    <name>C5DBW1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C-8 sterol isomerase</fullName>
            <ecNumber evidence="8">5.-.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Delta-8--delta-7 sterol isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0A05786g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the reaction which results in unsaturation at C-7 in the B ring of sterols.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7 8">Steroid metabolism; ergosterol biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ERG2 family.</text>
    </comment>
    <dbReference type="EC" id="5.-.-.-" evidence="8"/>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551710.1">
        <property type="nucleotide sequence ID" value="XM_002551664.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBW1"/>
    <dbReference type="FunCoup" id="C5DBW1">
        <property type="interactions" value="166"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBW1"/>
    <dbReference type="GeneID" id="8290514"/>
    <dbReference type="KEGG" id="lth:KLTH0A05786g"/>
    <dbReference type="eggNOG" id="KOG4143">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085469_0_0_1"/>
    <dbReference type="InParanoid" id="C5DBW1"/>
    <dbReference type="OMA" id="AMYVIHA"/>
    <dbReference type="OrthoDB" id="347124at2759"/>
    <dbReference type="UniPathway" id="UPA00768"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006716">
        <property type="entry name" value="ERG2_sigma1_rcpt-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10868">
        <property type="entry name" value="SIGMA 1-TYPE OPIOID RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10868:SF1">
        <property type="entry name" value="SIGMA NON-OPIOID INTRACELLULAR RECEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04622">
        <property type="entry name" value="ERG2_Sigma1R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU368083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR21268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="222" mass="25372" checksum="8A522333C1F36F1A" modified="2009-07-28" version="1">MKLLYVVGVLLSTYYIMDKLLYTWLPTNCVFDPDRLVLIANKVIATHNQMGNSTTEQLLMDVREELAKEYGDKWINPYEKDKWVFNNAGGAMGQMLILHASFSEYLILFGSATGNEGHSGVHFADDWFTILKGTQLASLPENPSPEVYNAGDVHLMRKGYAKQYSMARESYALEYARGWIPFMLPFGFLDTFTSTLDFYTLGKTVWITGRDMLRHLFVNGKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DC61</accession>
    <name>C5DC61_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0B00286p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0B00286g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551810.1">
        <property type="nucleotide sequence ID" value="XM_002551764.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DC61"/>
    <dbReference type="FunCoup" id="C5DC61">
        <property type="interactions" value="115"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DC61"/>
    <dbReference type="GeneID" id="8290629"/>
    <dbReference type="KEGG" id="lth:KLTH0B00286g"/>
    <dbReference type="eggNOG" id="KOG3179">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054974_0_2_1"/>
    <dbReference type="InParanoid" id="C5DC61"/>
    <dbReference type="OMA" id="DCFRVID"/>
    <dbReference type="OrthoDB" id="92161at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01741">
        <property type="entry name" value="GATase1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044992">
        <property type="entry name" value="ChyE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42695">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE YLR126C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42695:SF5">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE YLR126C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase" evidence="1">
        <location>
            <begin position="29"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="258" mass="28952" checksum="3A0669E766A9C7F1" modified="2009-07-28" version="1">MSKIAILNANPPQEALKRWGSFADMAIEMFEATKASFLNSTVEYEVYNVYEKDFPTVLELCNGDYLGLHITGSTSDAYAIDIEWVNILRKLLQNLLKNGAHPPISGICFGHQILASALGSEVRRNPKGYEGGITRLEITKEALKLGLFQNSTSPLPSCEFYASESHSDFVKNVPHGYINTLTSKKCDVQGLYKRNVALTFQCHPDFISEVCIKCNEGTYSRGEINADELAHMKESGRQRNNDGRFFATYIWMLFTNVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DC73</accession>
    <name>C5DC73_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0B00550p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0B00550g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551822.1">
        <property type="nucleotide sequence ID" value="XM_002551776.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DC73"/>
    <dbReference type="STRING" id="559295.C5DC73"/>
    <dbReference type="GeneID" id="8290641"/>
    <dbReference type="KEGG" id="lth:KLTH0B00550g"/>
    <dbReference type="eggNOG" id="KOG2348">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010778_2_1_1"/>
    <dbReference type="InParanoid" id="C5DC73"/>
    <dbReference type="OMA" id="VSHYFIC"/>
    <dbReference type="OrthoDB" id="6132759at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015489">
        <property type="term" value="F:putrescine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015606">
        <property type="term" value="F:spermidine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015204">
        <property type="term" value="F:urea transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11476">
        <property type="entry name" value="SLC5sbd_DUR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031155">
        <property type="entry name" value="DUR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00813">
        <property type="entry name" value="sss"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46154">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46154:SF4">
        <property type="entry name" value="UREA ACTIVE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="132"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="165"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="197"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="289"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="335"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="395"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="426"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="455"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="494"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="561"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="595"/>
            <end position="619"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR21383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="667" mass="72975" checksum="2770E1083B187D0E" modified="2009-07-28" version="1">MSEFVLNQGYGYGIVLGLGALFSVLMIALSWAIAKFLNQKQDSERFTTASRNVKQGLISSAVVSSWTWPATLLTSNLWTYSYGIVGAYWYSVTGCFQILVFTLVAIEIKLKCPGIHTIAEVAKVRFGKWGHFCYLFYSLGTNVIVSGMILLGGSQGIAYTTGMNIIAACFLLPVSVCIYTLFGGLQATFLSDWSHTIVIYIMVIVSIVTVYCTSDLIGSPDKMYDLLKDMGESFPLESSANGSYLTFANKDIILTAWNALLGGAATVFMDPSYSQKAIAAGSKEVMRGYMLGGICWQIIPLGLGTSMGLASLALTKNPAFFTYPNKLTDLEAGRGLSLVYAMTAIFGKSGAACAVVMVFMSATSAMSAELISCSSILTYDFYKTYVNPKASGKQLVYVSHLNVIGFSLCMGALAVVFNYIGVTLGWLFSFVGIALGPAFFGLLGMLFYKKMNTMALVVGVPSATLCGIVGWVASATRYSSDGRVDKASLSLSEPLAFGNFISVFSSGIFVWILSQVKSQSWNFNEMNETYFETQIADDADSDEIEFSQVSKESVKDLRKHIFITKILVFCQFIIFLIIIPLSMYGTKYIFSRKLFRGYVCIIIIWAFLAAMWIIFYPLYESKSNLIFVFKKLIGKASTDSSADLSQTDTSSIEREKLDKTSINVAAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DC96</accession>
    <name>C5DC96_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0B01232p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0B01232g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Spindle pole body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TOG/XMAP215 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551845.1">
        <property type="nucleotide sequence ID" value="XM_002551799.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DC96"/>
    <dbReference type="FunCoup" id="C5DC96">
        <property type="interactions" value="111"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DC96"/>
    <dbReference type="GeneID" id="8290671"/>
    <dbReference type="KEGG" id="lth:KLTH0B01232g"/>
    <dbReference type="eggNOG" id="KOG1820">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008401_1_0_1"/>
    <dbReference type="InParanoid" id="C5DC96"/>
    <dbReference type="OMA" id="HNFGCKI"/>
    <dbReference type="OrthoDB" id="205662at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005881">
        <property type="term" value="C:cytoplasmic microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990498">
        <property type="term" value="C:mitotic spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044732">
        <property type="term" value="C:mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099070">
        <property type="term" value="C:static microtubule bundle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061863">
        <property type="term" value="F:microtubule plus end polymerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051010">
        <property type="term" value="F:microtubule plus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051315">
        <property type="term" value="P:attachment of mitotic spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030951">
        <property type="term" value="P:establishment or maintenance of microtubule cytoskeleton polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990571">
        <property type="term" value="P:meiotic centromere clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046785">
        <property type="term" value="P:microtubule polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000022">
        <property type="term" value="P:mitotic spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000019">
        <property type="entry name" value="Cytoskeleton-associated protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048492">
        <property type="entry name" value="Stu2_CTS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045110">
        <property type="entry name" value="XMAP215"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048491">
        <property type="entry name" value="XMAP215_CLASP_TOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12609">
        <property type="entry name" value="MICROTUBULE ASSOCIATED PROTEIN XMAP215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21042">
        <property type="entry name" value="Stu2_CTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21041">
        <property type="entry name" value="XMAP215_CLASP_TOG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="TOG" evidence="9">
        <location>
            <begin position="3"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="9">
        <location>
            <begin position="299"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="6">
        <location>
            <begin position="477"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="546"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="737"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="702"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="563"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="609"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="737"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="769"/>
            <end position="784"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR21407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="866" mass="96486" checksum="E5A270BD0FD5F5BB" modified="2009-07-28" version="1">MADSEDGDFTSLPLSERLDHKLWKARLHGYQELQALFQKASVTSVPRELAQYWANPDLFAGYIVDSNVVAQEQAITALQNMLEYLCEFRDTPRSDEMRGRWLPLLAEKGLSSSRNSTKAKAQECVLILVSLDRSVEHSIELLEPSLTNKLPRLVANCVECISNIVESFGLAQVSNMSGFLPVLLEPLPKLSSHADRNVRSQTMNLILQLYKWLGRDLLQELLLEKLKPIQQRDLDKTFGKYDGEIPPTSQPRPFQWQKGLEVQNLEGSKDKDGDTLMGESFPDVKAHANPGLPQLDPFELLPVSPVLEKFPLDFMERITSSKWKDRVEVLEEILNQVLIPTKKFEAKGQDYSDFLRAVAHVIEKDANVQAVTLAAQCVQQLCQKLKANFTRSYGSIVLRSLLERSKEKKASVNEAICEALNNIADCCGVDACLEDNLSFINHKTPQVRTESTRFLTRLLKKWTPEGRHFGDELLIKLVPDISQMLLKIVGDTQPTVRDAGFECLATLMKLVGEREVSDVLDKLDNLKKKKVYEHYEKVEVAGGATKGAVTSAPARREKPSMPYTPNASRRQAPRTTPTPDLSAQATSGSTLPLRKPLSTIPSKRGPSSPLKTGTRVETYQDGASSMKSRLTARSLTTDSAPASKPQAAPHAGFEELQRQKQKWLKERQEFLATITDFQNKASHLSSENSSLRQQLDTVQSSLHESNIELRSKEMQITRLQDRISNLEQELEAKSTLPSSNYGVRSASSFEPPSAPLSLAPPGTPPTKRVFSSSSNGRASPSFDANSDRIADSRLPSVGSRLRSPSECSDDLPHRVNSLQLRSAERAATTGNSFLNDESWKRAAEVTSQLKARIERMRAKTRGMGNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCQ8</accession>
    <name>C5DCQ8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0B05060p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0B05060g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552007.1">
        <property type="nucleotide sequence ID" value="XM_002551961.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCQ8"/>
    <dbReference type="GeneID" id="8290845"/>
    <dbReference type="KEGG" id="lth:KLTH0B05060g"/>
    <dbReference type="HOGENOM" id="CLU_637892_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCQ8"/>
    <dbReference type="OrthoDB" id="4033174at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="273"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="224"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="326"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="41"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR21569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="432" mass="49833" checksum="7DAEB786F506C394" modified="2009-07-28" version="1">MESGKRQSINSPARPAPREISCSETLLNDGDDDAQANSTEIDNHDMETGRDVSPSPRNLVGTTSHDEMQQEQLYLEYFHSAIITEELGLIKHLKRAHGKMIKAIQELIRVKGYRSADSGKEYRFKWTDNATYYLLKYCLEIGPFYGFEHLKRPSLTKLEQSSLISLKWSCVWYDLLMTLEFENSIIPTESQIKSKCRYLLDNAHPKLFAVTTGDERNPFSFTGVDKVDNNLEMLTRLKNDAEQRKVEIQKLETDRQNNKRTLGQMLCSNSIPSVTEGLSNSDSEEPSSIDDINQPNSITMRRNKRMGRLLDFKEIAQYSLSNSALVGDLQETLKELIEAAAVEERKYKDKEQQRKDQELALKQKKLEISLQHNQRMLELELERQKREQFDHDAKKIDTLVKMMDRAKSLEGDKSPSSEALQKLLHNFVSRYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCR5</accession>
    <name>C5DCR5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0B05214p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0B05214g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL35 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552014.1">
        <property type="nucleotide sequence ID" value="XM_002551968.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCR5"/>
    <dbReference type="FunCoup" id="C5DCR5">
        <property type="interactions" value="81"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCR5"/>
    <dbReference type="GeneID" id="8290852"/>
    <dbReference type="KEGG" id="lth:KLTH0B05214g"/>
    <dbReference type="eggNOG" id="ENOG502S7SR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_166987_1_0_1"/>
    <dbReference type="InParanoid" id="C5DCR5"/>
    <dbReference type="OMA" id="PAHESHI"/>
    <dbReference type="OrthoDB" id="162638at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001706">
        <property type="entry name" value="Ribosomal_bL35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021137">
        <property type="entry name" value="Ribosomal_bL35-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037229">
        <property type="entry name" value="Ribosomal_bL35_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33343">
        <property type="entry name" value="54S RIBOSOMAL PROTEIN BL35M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33343:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL35M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01632">
        <property type="entry name" value="Ribosomal_L35p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143034">
        <property type="entry name" value="L35p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="40"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="40"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR21576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="91" mass="10536" checksum="3394297A09656AAC" modified="2009-07-28" version="1">MLSNLMRSIMSYGRQITQLQQPFMMQTRNLMKTHKGTAKRWRKTSTSYKRGRAGRNHGNAGWGKSTLKSLDGKTLAHPTHIKHLKRLLPYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCU5</accession>
    <name>C5DCU5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0B05896p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0B05896g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552044.1">
        <property type="nucleotide sequence ID" value="XM_002551998.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCU5"/>
    <dbReference type="FunCoup" id="C5DCU5">
        <property type="interactions" value="871"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCU5"/>
    <dbReference type="GeneID" id="8290883"/>
    <dbReference type="KEGG" id="lth:KLTH0B05896g"/>
    <dbReference type="eggNOG" id="KOG1781">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076902_3_1_1"/>
    <dbReference type="InParanoid" id="C5DCU5"/>
    <dbReference type="OMA" id="PFVQQEE"/>
    <dbReference type="OrthoDB" id="274944at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990726">
        <property type="term" value="C:Lsm1-7-Pat1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097526">
        <property type="term" value="C:spliceosomal tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005688">
        <property type="term" value="C:U6 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01729">
        <property type="entry name" value="LSm7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000098">
        <property type="entry name" value="U6 snRNA-associated Sm-like protein LSm7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017132">
        <property type="entry name" value="Lsm7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044641">
        <property type="entry name" value="Lsm7/SmG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10553">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10553:SF5">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037188">
        <property type="entry name" value="U6_snRNA_Lsm7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="9">
        <location>
            <begin position="17"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR21606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="111" mass="12403" checksum="7D7F6E9DA87B9872" modified="2009-07-28" version="1">MSEVQQSKRKFEGPKREAIVNLSKYADNKIRVKLAGGRLVVGVLKGYDQLMNLVLDETVEHLRDPEDDTVILEGQTRELGLVVIRGTMLLTLSPMDGSEIISNPHGPEEEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCV0</accession>
    <name>C5DCV0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0B06006p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0B06006g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the arginase family. Agmatinase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552049.1">
        <property type="nucleotide sequence ID" value="XM_002552003.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCV0"/>
    <dbReference type="STRING" id="559295.C5DCV0"/>
    <dbReference type="GeneID" id="8290888"/>
    <dbReference type="KEGG" id="lth:KLTH0B06006g"/>
    <dbReference type="eggNOG" id="KOG2964">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039478_1_1_1"/>
    <dbReference type="InParanoid" id="C5DCV0"/>
    <dbReference type="OMA" id="YELTTIM"/>
    <dbReference type="OrthoDB" id="288726at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008783">
        <property type="term" value="F:agmatinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033389">
        <property type="term" value="P:putrescine biosynthetic process from arginine, via agmatine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11592">
        <property type="entry name" value="Agmatinase_PAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.800.10:FF:000014">
        <property type="entry name" value="Arginase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.10">
        <property type="entry name" value="Ureohydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006035">
        <property type="entry name" value="Ureohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020855">
        <property type="entry name" value="Ureohydrolase_Mn_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358">
        <property type="entry name" value="ARGINASE/AGMATINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358:SF26">
        <property type="entry name" value="GUANIDINO ACID HYDROLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00491">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00116">
        <property type="entry name" value="ARGINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01053">
        <property type="entry name" value="ARGINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51409">
        <property type="entry name" value="ARGINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950319" evidence="5">
        <location>
            <begin position="19"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR21611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="412" mass="45599" checksum="BF8A05EE4DAA4B72" modified="2009-07-28" version="1">MLMRSVLFSLACANAVLASGTTVLEEDLEQMWGQDWPFSGIQTFAHLPHEKCLMNRSLDFDIGVIGIPFDTAVTYRPGARFGPQAIRKSSQRQTSMRGFNFRAGINPYQDWAKVLDCGDVPVTPMDNNLALQMMGAAYHNLLNRNSTLKQAELPPRFATLGGDHSIILPILRQLHKIYGPISVIHFDSHLDTWAPSKYPSYWHSNNSDFTHGSMLWIAKQEGLLSENSNVHAGLRTRLSGVGWDDYEEDKETGFHRIECDEILDIGVRGIARKILDIVPKDKPVYISVDIDVLDPSAAPGTGTVEVGGLLTRELISIIRQLDGLSLIGADVVEVSPAYDQSDITSTAASQIVYELITNMVKKGPLDPAMIQANKNSEMDQGDKPQNLAENEYQSLGEGARQNFMESLLQGRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCX6</accession>
    <name>C5DCX6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0B06578p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0B06578g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552075.1">
        <property type="nucleotide sequence ID" value="XM_002552029.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCX6"/>
    <dbReference type="FunCoup" id="C5DCX6">
        <property type="interactions" value="18"/>
    </dbReference>
    <dbReference type="GeneID" id="8290914"/>
    <dbReference type="KEGG" id="lth:KLTH0B06578g"/>
    <dbReference type="eggNOG" id="ENOG502QR9U">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065220_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCX6"/>
    <dbReference type="OMA" id="GLNLRWY"/>
    <dbReference type="OrthoDB" id="4063705at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="381" mass="43630" checksum="A06A710649D49163" modified="2009-07-28" version="1">MDRVRNLVRRSNGSRANRENNASVLRDPVASDSDQATLVESKRSDERRSHNEEGVTLQRRNSETSTIGDLRVSGFVNRCPLFHDLRHIVFASTVMGKGFYAFPSIESFQKFRENKRRLDELHDEQALGFPLFHAVPVNVFRTIIKRSLPMMKIYRFVLVDTSVPGEAEKYQENETCTLMAQLGATCVYKLEFCQVYQRIHTNTSFVEYKFTFRIDNRPENNLFSMRMVNHVHRRDTDSRINDLNLRWLGTSGLASPFGSGTFQLAVLDDGIASYLDELPGSSREQLGRAADQPRSPWQMPVWATYSDGKATFIPKKRTLRVADFKIKELAQCCQGLQDVPWDTLVLTCMCMVLHDLEARKDRRSMNVTAGNDMALASFSFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DD20</accession>
    <name>C5DD20_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serine/threonine-protein phosphatase 4 regulatory subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0B07634g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Core regulatory subunit of the histone H2A phosphatase complex, which dephosphorylates H2AS128ph (gamma-H2A) that has been displaced from sites of DNA lesions in the double-stranded DNA break repair process. Dephosphorylation is necessary for efficient recovery from the DNA damage checkpoint.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552119.1">
        <property type="nucleotide sequence ID" value="XM_002552073.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DD20"/>
    <dbReference type="FunCoup" id="C5DD20">
        <property type="interactions" value="969"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DD20"/>
    <dbReference type="GeneID" id="8290959"/>
    <dbReference type="KEGG" id="lth:KLTH0B07634g"/>
    <dbReference type="eggNOG" id="KOG2175">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004909_4_0_1"/>
    <dbReference type="InParanoid" id="C5DD20"/>
    <dbReference type="OMA" id="ALMTHNN"/>
    <dbReference type="OrthoDB" id="27483at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030289">
        <property type="term" value="C:protein phosphatase 4 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072542">
        <property type="term" value="F:protein phosphatase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010948">
        <property type="term" value="P:negative regulation of cell cycle process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000455">
        <property type="entry name" value="Psy2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055236">
        <property type="entry name" value="EVH1_PP4R3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006887">
        <property type="entry name" value="P4R3-like_central_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051137">
        <property type="entry name" value="PP4R3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23318">
        <property type="entry name" value="ATP SYNTHASE GAMMA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23318:SF0">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 4 REGULATORY SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22972">
        <property type="entry name" value="EVH1_PP4R3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04802">
        <property type="entry name" value="PP4R3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PP4R3 EVH1-like" evidence="6">
        <location>
            <begin position="15"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Serine/threonine-protein phosphatase 4 regulatory subunit 3-like central" evidence="5">
        <location>
            <begin position="170"/>
            <end position="721"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00058488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00068937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF22972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR21681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="789" mass="90368" checksum="98E1A8BFEB129005" modified="2009-07-28" version="1">MNGVRQDELRETESKRVKVYILEQNEWKDTGTGFCIGKVDKEERQAEQNAPPEDALAAYLVVNDEEAPEKTLLWSKLEGNIEYQRQEETLIVWKDLSGQDIALSFEESTGCDALCDFIIKIQKSSENNISLVAVRSNDDGMGSVHEIITGPVNLPCNDAEQDEKQLMEALKILNENTAFEYLRSETMEFVLKSNYVATLIKLFEKAEAGHKLRELLLLSNILKTLTLYNQRELLEQMVGDEYIMGVVGILEYDTEFPTSKANHREYLLHSGPHFKEVVPLNSDDMKRIIKKTYRLQFFKDVVLVRFLDEHSFNIISDIILDLQTCIINFIQDETFLPKLIALYTAASPDTPIETKRDGIRMLHQCVQITKNLEHSEKSRFFKALVRAGLFKIFDFAFGQETDSGIRILATDMVISIIEHDILLINSVQNELINQPEGLDQEQQHSHKQPAENGNCTVSSDVSLLAILSKILLTDKSLGLKEQVVQALNTLLHPEGCMGMDPDYNDSGGVDMMMNFNDARSNGMNEENLIDNMNFQMTDYFNNFYTQVAPQLFAPLITNSLSTPDDDVLLIYLVKLINFVSTEHNRRLSREFIMENNILKNIAKLLDPQRMLQLRLTVVRCLKSIVCLNDEFYHRYLISENLYAPVMDLLRENLHEDNLANSSILDFFKIVSSQCMQALELKASDKRNFIMLNKHLVKNYRDILLEVDYVPFTKDMIRMYDEGEGQLRESVTESTSMVYAKEGKRTFSETLETTKVSTSEETTTFKKAMEKITSAPPQSFIKEDVLGTEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DD21</accession>
    <name>C5DD21_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">2,4-dienoyl-CoA reductase [(3E)-enoyl-CoA-producing]</fullName>
            <ecNumber evidence="3">1.3.1.124</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0B07656g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a (2E,4E)-dienoyl-CoA + NADPH + H(+) = a 4,5-saturated-(3E)-enoyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:45912"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:85101"/>
            <dbReference type="ChEBI" id="CHEBI:85493"/>
            <dbReference type="EC" id="1.3.1.124"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a (2E,4Z)-dienoyl-CoA + NADPH + H(+) = a 4,5-saturated-(3E)-enoyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:61892"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:85099"/>
            <dbReference type="ChEBI" id="CHEBI:85493"/>
            <dbReference type="EC" id="1.3.1.124"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="1.3.1.124" evidence="3"/>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552120.1">
        <property type="nucleotide sequence ID" value="XM_002552074.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DD21"/>
    <dbReference type="FunCoup" id="C5DD21">
        <property type="interactions" value="272"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DD21"/>
    <dbReference type="GeneID" id="8290960"/>
    <dbReference type="KEGG" id="lth:KLTH0B07656g"/>
    <dbReference type="eggNOG" id="KOG0725">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_2_1"/>
    <dbReference type="InParanoid" id="C5DD21"/>
    <dbReference type="OMA" id="QLRDWSD"/>
    <dbReference type="OrthoDB" id="2136131at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008670">
        <property type="term" value="F:2,4-dienoyl-CoA reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009062">
        <property type="term" value="P:fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05369">
        <property type="entry name" value="TER_DECR_SDR_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045017">
        <property type="entry name" value="DECR2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43296">
        <property type="entry name" value="PEROXISOMAL 2,4-DIENOYL-COA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43296:SF2">
        <property type="entry name" value="PEROXISOMAL 2,4-DIENOYL-COA REDUCTASE [(3E)-ENOYL-COA-PRODUCING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00026117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR21682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="300" mass="31871" checksum="1F9DFD754315CE3E" modified="2009-07-28" version="1">MPNSLGDSFVKDGPWKADLFQGKVAFVTGGAGTICRVQTEALVLLGCKAAIVGRNPEKTLEAAREISKLSPRRDSTENDSVGGDSVLAIHSVDVREIGQLEQAVARTVERFGRIDFVIAGAAGNFIAPFSQLSANAFKSVVSIDLLGSFNTAKACLAELKKNKGAILFVSATFHYYGVPFQSHVGAAKAGVDALSNALAVELGVSGIRSNCIAPGVIEGTEGVSRLTGNVSEEQIRSKIPLQRFGTTRDIAETTVYLFSDAAKYVTGTVHIVDGGMWHLGTHFGRDLYPQNLLTEENAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDF1</accession>
    <name>C5DDF1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0C00506p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0C00506g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552250.1">
        <property type="nucleotide sequence ID" value="XM_002552204.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDF1"/>
    <dbReference type="FunCoup" id="C5DDF1">
        <property type="interactions" value="99"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDF1"/>
    <dbReference type="GeneID" id="8291108"/>
    <dbReference type="KEGG" id="lth:KLTH0C00506g"/>
    <dbReference type="eggNOG" id="KOG1204">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_2_11_1"/>
    <dbReference type="InParanoid" id="C5DDF1"/>
    <dbReference type="OMA" id="SHVDEWR"/>
    <dbReference type="OrthoDB" id="153074at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050664">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05367">
        <property type="entry name" value="SPR-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43008">
        <property type="entry name" value="BENZIL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43008:SF8">
        <property type="entry name" value="BENZIL REDUCTASE ((S)-BENZOIN FORMING) IRC24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR21812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="274" mass="29893" checksum="506C23DC052C9728" modified="2009-07-28" version="1">MSKVVLVTGASKGIGRAIVDVLCSSGEDIKVLGIARSVDDLEALKTKHQGKFDYLCADITDTAAIVGYVKKINKNYGSLDAVIANAGVLAPVQSVARADVMQWKQLFDVNFFSIVSLVSELLPSLKKSSQGDVILVSSGASVKPYVCDKHHNRFAKEITTNNQKQYAWGAYGASKAALNQFAQILALEEPQIKTVALAPGVVNTQMQDAIRDELGPPNMTPEALKRFTDLREKNELLEPEIPATIYANLALHGIPATINGKYLRYNDELLKEYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-04-02" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDP4</accession>
    <name>C5DDP4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0C02640p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0C02640g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552343.1">
        <property type="nucleotide sequence ID" value="XM_002552297.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDP4"/>
    <dbReference type="GeneID" id="8291203"/>
    <dbReference type="KEGG" id="lth:KLTH0C02640g"/>
    <dbReference type="eggNOG" id="KOG1318">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046871_0_1_1"/>
    <dbReference type="InParanoid" id="C5DDP4"/>
    <dbReference type="OrthoDB" id="71302at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47787">
        <property type="entry name" value="CENTROMERE-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47787:SF1">
        <property type="entry name" value="CENTROMERE-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BHLH" evidence="2">
        <location>
            <begin position="142"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="63"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR21905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="262" mass="29277" checksum="040D0919D9A793EB" modified="2009-07-28" version="1">MAKRLLPNDVEVGNDELKRQRAEAAEQSNIDAELLKEKQDPASGDEDVHRDEDEDDQDHDGTAAAAAVAAAAYGGFMGGQDDPNHELHHAHHAHHHHDVHDAKVEDDEDDDDASLKDEDGKRSGRRGRKPAPVTGSAEWRQQRKDSHKEVERRRRENINTAINKLSELLPVKESSKASILLRAAEYILKLKDTENANIEKWTLQKLLGEQQVSTLTKANESLQLELGNAFKQVDILKRQLRNAGIAVDPEIAKLEAEATRNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDR5</accession>
    <name>C5DDR5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0C03190p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0C03190g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552364.1">
        <property type="nucleotide sequence ID" value="XM_002552318.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDR5"/>
    <dbReference type="FunCoup" id="C5DDR5">
        <property type="interactions" value="96"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDR5"/>
    <dbReference type="GeneID" id="8291228"/>
    <dbReference type="KEGG" id="lth:KLTH0C03190g"/>
    <dbReference type="eggNOG" id="KOG4137">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071116_2_1_1"/>
    <dbReference type="InParanoid" id="C5DDR5"/>
    <dbReference type="OMA" id="VIKPMAP"/>
    <dbReference type="OrthoDB" id="49520at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029525">
        <property type="entry name" value="INO80C/Ies6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013272">
        <property type="entry name" value="Vps72/YL1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31200">
        <property type="entry name" value="INO80 COMPLEX SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31200:SF1">
        <property type="entry name" value="INO80 COMPLEX SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08265">
        <property type="entry name" value="YL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00993">
        <property type="entry name" value="YL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Vps72/YL1 C-terminal" evidence="5">
        <location>
            <begin position="82"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR21926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="134" mass="15259" checksum="284A10F1DB3D19D4" modified="2009-07-28" version="1">MPPSNSPGPMDRIEFLREVAASNSTDGLHLKRASWKKPHRRHKATRQLVTDEWKRLSNTPSEANRLTYFNVEAPPSIYPAKKYCDITGLKASYRAPGNGLRFYNSEVYSLVIKPMAPGVDQQYLRLRGDDIVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEA7</accession>
    <name>C5DEA7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0C07656p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0C07656g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552556.1">
        <property type="nucleotide sequence ID" value="XM_002552510.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEA7"/>
    <dbReference type="FunCoup" id="C5DEA7">
        <property type="interactions" value="419"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEA7"/>
    <dbReference type="GeneID" id="8291428"/>
    <dbReference type="KEGG" id="lth:KLTH0C07656g"/>
    <dbReference type="eggNOG" id="ENOG502QSII">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012586_0_0_1"/>
    <dbReference type="InParanoid" id="C5DEA7"/>
    <dbReference type="OMA" id="WKYIILK"/>
    <dbReference type="OrthoDB" id="5563539at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042149">
        <property type="term" value="P:cellular response to glucose starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007039">
        <property type="term" value="P:protein catabolic process in the vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013860">
        <property type="entry name" value="AreA_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052292">
        <property type="entry name" value="Glucose_repression_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28051">
        <property type="entry name" value="PROTEIN MTL1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28051:SF1">
        <property type="entry name" value="PROTEIN MTL1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08550">
        <property type="entry name" value="GATA_AreA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitrogen regulatory protein areA GATA-like" evidence="2">
        <location>
            <begin position="156"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="217"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="319"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="395"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="460"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="562"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="659"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="241"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="347"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="396"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="409"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="468"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="596"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="707"/>
            <end position="729"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR22118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="853" mass="93565" checksum="8067223C71876E9A" modified="2009-07-28" version="1">MSNNLATYFNKQAGRSSTTSGASPGDDDDMGPSVSMAVEAEDDDHFTRSTFNLKRTRSMGLLDDYVGSSKKDEGSEADSEEAAEESDFEDDDNVNENAGSSALRSATPGLSESASPPVPETDNYLIPHDDNDLVHEPKRHVDYLSHQWDEGEISQSWKYIILKKKKKDEDLVNAARLENASWRTWAKARNHLQTVSPEVVNWSKDSDVTWLYGPVVRQKTDPGASSDQEFGYGSDDETSKRVRTSKNKKRGPKPILKKRSVSEILEENSLWRLNVARQHKKILSSTQDMVDGYGDTMYAPEDYDALAAKVNAQYYSSNPSAYSQGNASEPLHEDPGVESSVGPPSERPQSQASTLESIMTSTSKKTPKGATEQKDRHIHFNDRVEQCMAVAFHSDDDNDFDDSADDSDGERSAHHEYPLTRNRTRGASDDSDYIRESYSDEDMNSSSSDEDEAGLFINARYSRKTDGSRSPSTRSSIGSESALSKENLTPIIKLLPATTLNYGSDEEEFDDDDEYYGNNAVSHNVNTFRGYDYMYDYNSVYTGDTSSFMAVNSCDMVDVPEGLQTPIEGQGDHADMLVNPNSSPQLPSDAGKGGFSLDSDTDSYGSDGDAQFIEDSQNKDSDDELSSMPGLRRVPSHGKSSTTLIQDLRLQPAAIPTTHSFITGKPLRQTEQPWDTVKSKPVPKTRSFVLDSDSDSDTSDKSESGSENSDINKTSLQAGDTPAANSSGADSKPAYSVPTHATIVSPVPKGQRITSSSPVNIACSGDSGTSLSDVAIAGYISPRSDSMQSVVAKENMVNNKSSNSEVDRMNKNLENWHIDPQKDDADDASSENVQKMMSSAREIASKYLHSWKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEC0</accession>
    <name>C5DEC0_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ATP-dependent RNA helicase FAL1</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">ATP-dependent RNA helicase fal1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">KLTH0C07964g</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552569.1">
        <property type="nucleotide sequence ID" value="XM_002552523.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEC0"/>
    <dbReference type="FunCoup" id="C5DEC0">
        <property type="interactions" value="728"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEC0"/>
    <dbReference type="GeneID" id="8291442"/>
    <dbReference type="KEGG" id="lth:KLTH0C07964g"/>
    <dbReference type="eggNOG" id="KOG0328">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_1_0_1"/>
    <dbReference type="InParanoid" id="C5DEC0"/>
    <dbReference type="OMA" id="DTIHGDK"/>
    <dbReference type="OrthoDB" id="10265785at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd17939">
        <property type="entry name" value="DEADc_EIF4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000849">
        <property type="entry name" value="ATP-dependent RNA helicase DBP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000031">
        <property type="entry name" value="Eukaryotic initiation factor 4A-III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 13" id="KW-0347">Helicase</keyword>
    <keyword evidence="4 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="16">
        <location>
            <begin position="23"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="54"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="15">
        <location>
            <begin position="254"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="12">
        <location>
            <begin position="23"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAR22131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="398" mass="44631" checksum="6CE40CBB9897FBF9" modified="2009-07-28" version="1">MSFSKDSDSKLKFKTSGKLKVSPTFESMHLKEDLLRGVYAYGFEAPSAIQSRAITQIISGRDVIAQAQSGTGKTATFTIGMLQVIDFKSRELQSLVLSPTRELAKQISQVVGNLGDYMNISAHACTGGKAMQTDTKKLTHGCHVVSGTPGRVLDMIKRRILNTRHLKMLILDEADELLSETLGFKQQIYDIFAKLPKSVQVVVVSATMSKDILEITKKFMSDPVKILVKRDEISLEGIKQYHVNVDREEWKFDTLCDLYDSLTITQCVIFCNTKKVVDWLSSKLLQANFAVASMHGDMKQEDRDRVMNDFRSGTSRVLISTDVWARGIDVQQVSLVINYDLPDNLENYIHRIGRSGRFGRKGVAINFITRDDAQGLKAIEKHYSVKIRPMPANLAELS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5DED1</accession>
    <name>C5DED1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DASH complex subunit DAD1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Outer kinetochore protein DAD1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">KLTH0C08206g</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
            <location evidence="3">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DASH complex DAD1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552580.1">
        <property type="nucleotide sequence ID" value="XM_002552534.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DED1"/>
    <dbReference type="FunCoup" id="C5DED1">
        <property type="interactions" value="41"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DED1"/>
    <dbReference type="GeneID" id="8291453"/>
    <dbReference type="KEGG" id="lth:KLTH0C08206g"/>
    <dbReference type="eggNOG" id="ENOG502SBWQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_142427_2_2_1"/>
    <dbReference type="InParanoid" id="C5DED1"/>
    <dbReference type="OMA" id="ENVSELW"/>
    <dbReference type="OrthoDB" id="5566853at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042729">
        <property type="term" value="C:DASH complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044732">
        <property type="term" value="C:mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005876">
        <property type="term" value="C:spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051010">
        <property type="term" value="F:microtubule plus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013958">
        <property type="entry name" value="DASH_Dad1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28025">
        <property type="entry name" value="DASH COMPLEX SUBUNIT DAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28025:SF1">
        <property type="entry name" value="DASH COMPLEX SUBUNIT DAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08649">
        <property type="entry name" value="DASH_Dad1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="15" id="KW-0137">Centromere</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="9" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00030566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAR22142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="97" mass="10760" checksum="0FD2D65EF95FF933" modified="2009-07-28" version="1">MSEKSRKGEDAAPVLSEGDKYFIEQREMILQDIGQTMDSILNNLNGLNISLENFIAVGKEFENVSELWSTFYTGISGEAEAVDTVAVNEQENLAPEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>C5DED6</accession>
    <name>C5DED6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0C08338p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0C08338g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552585.1">
        <property type="nucleotide sequence ID" value="XM_002552539.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DED6"/>
    <dbReference type="FunCoup" id="C5DED6">
        <property type="interactions" value="133"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DED6"/>
    <dbReference type="GeneID" id="8291459"/>
    <dbReference type="KEGG" id="lth:KLTH0C08338g"/>
    <dbReference type="eggNOG" id="ENOG502RZ2K">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076154_2_0_1"/>
    <dbReference type="InParanoid" id="C5DED6"/>
    <dbReference type="OMA" id="KGHKHEL"/>
    <dbReference type="OrthoDB" id="18529at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037507">
        <property type="entry name" value="Ribosomal_mL59"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040922">
        <property type="entry name" value="Ribosomal_mL59_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28041">
        <property type="entry name" value="54S RIBOSOMAL PROTEIN L25, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28041:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18126">
        <property type="entry name" value="Mitoc_mL59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Large ribosomal subunit protein mL59" evidence="1">
        <location>
            <begin position="16"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="157" mass="18679" checksum="27B7CF7DAF1ADE82" modified="2009-07-28" version="1">MSSAPLKQYFQLLPDKLRTFFKKYPPNVKYSDKATSTGAIDANPFLANKHPITGKYHNPKYSLRRMSDLYKLAHQYGLQELLPPSNKLFFDEKYEKKKLMKGVLIPKGHKHELAQSEKMQKMREAIKNADQYILEVKGRKYMKKLEKRKEEKRPRWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>C5DER3</accession>
    <name>C5DER3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0C11440p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0C11440g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552712.1">
        <property type="nucleotide sequence ID" value="XM_002552666.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DER3"/>
    <dbReference type="FunCoup" id="C5DER3">
        <property type="interactions" value="51"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DER3"/>
    <dbReference type="GeneID" id="8291599"/>
    <dbReference type="KEGG" id="lth:KLTH0C11440g"/>
    <dbReference type="eggNOG" id="ENOG502QTAU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043707_0_0_1"/>
    <dbReference type="InParanoid" id="C5DER3"/>
    <dbReference type="OMA" id="HLENYFA"/>
    <dbReference type="OrthoDB" id="3979966at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052058">
        <property type="entry name" value="Alcohol_O-acetyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010828">
        <property type="entry name" value="Atf2/Sli1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28037">
        <property type="entry name" value="ALCOHOL O-ACETYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28037:SF1">
        <property type="entry name" value="ALCOHOL O-ACETYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07247">
        <property type="entry name" value="AATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR22274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="508" mass="58452" checksum="61062573DC8818A3" modified="2009-07-28" version="1">MDSLKERGHARPLGHLENYFSITQRQKLYANFSMYCELSKPCSPKQLAYALRSICLQNPILVHQVLPKHWPNHLEYYASDEFLAQPTLQHEDMRLLDNVLLSDIVMNEQEEYGTVVSEAIEEFSQNGGQYSKKIFDIIADIRIPYGDPLKPNWRLLCFPEGESNLWRKFIYITNHCSSDGRSAANLMRDLSEQLNHVPETLPDSDIIFNYSSDYEGLRKLPDPIENRIDYKPPISYLLQLLSSSYVRDYLGYYSKGPLVTRIDEVGENKTYYSYFLNFSAEQMKTIKQKLKSRLPGCTMTPFLQACWLTSMYKSGRVFSKSMREWFFDVVITMNTAQMLPDDPELRSMYKYGSNVGGTRYNYLISSFNVGEDKDAFWSLVDYYQGVFNSAMEKKHYLFPLGALMLDSLREKSNMDKVIMDDLLGKPRQGVILSNVGYFQQKKETDGYYVRDLVFAQSLGSLRHTFVCNSCTTDVGGMNIVACAAQGSVASEHDWADVCELFKEQTLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEX6</accession>
    <name>C5DEX6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Iron-sulfur cluster assembly protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0D10560g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Scaffold protein for the de novo synthesis of iron-sulfur (Fe-S) clusters within mitochondria, which is required for maturation of both mitochondrial and cytoplasmic [2Fe-2S] and [4Fe-4S] proteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8 9">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; iron-sulfur cluster biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the NifU family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553169.1">
        <property type="nucleotide sequence ID" value="XM_002553123.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEX6"/>
    <dbReference type="FunCoup" id="C5DEX6">
        <property type="interactions" value="430"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEX6"/>
    <dbReference type="GeneID" id="8295408"/>
    <dbReference type="KEGG" id="lth:KLTH0D10560g"/>
    <dbReference type="eggNOG" id="KOG3361">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079283_5_0_1"/>
    <dbReference type="InParanoid" id="C5DEX6"/>
    <dbReference type="OMA" id="SMVTEMV"/>
    <dbReference type="OrthoDB" id="1925777at2759"/>
    <dbReference type="UniPathway" id="UPA00266"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06664">
        <property type="entry name" value="IscU_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1010.10:FF:000005">
        <property type="entry name" value="Iron-sulfur cluster assembly protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011339">
        <property type="entry name" value="ISCU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002871">
        <property type="entry name" value="NIF_FeS_clus_asmbl_NifU_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01999">
        <property type="entry name" value="iscU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10093">
        <property type="entry name" value="IRON-SULFUR CLUSTER ASSEMBLY ENZYME NIFU HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01592">
        <property type="entry name" value="NifU_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82649">
        <property type="entry name" value="SufE/NifU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="6 9" id="KW-0408">Iron</keyword>
    <keyword evidence="7 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="NIF system FeS cluster assembly NifU N-terminal" evidence="10">
        <location>
            <begin position="45"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR22731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="175" mass="18789" checksum="B221B96B164FAAC5" modified="2009-07-28" version="1">MFRTLLARNSLGASARSVLRAPVPVTGAAVPRFVGNPVAQPARRAYHPKVIDHYTNPRNVGSLDKNLENVGTGLVGAPACGDVMKLQIQVNDDTGVIENVKFKTFGCGSAIASSSYMTELVRGKTLDVASQIKNTEIAKELSLPPVKLHCSMLAEDAIKAAIKDYRSKRKSTVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEY0</accession>
    <name>C5DEY0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0D10648p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0D10648g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553173.1">
        <property type="nucleotide sequence ID" value="XM_002553127.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEY0"/>
    <dbReference type="FunCoup" id="C5DEY0">
        <property type="interactions" value="405"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEY0"/>
    <dbReference type="GeneID" id="8295412"/>
    <dbReference type="KEGG" id="lth:KLTH0D10648g"/>
    <dbReference type="eggNOG" id="ENOG502QSEV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036523_0_0_1"/>
    <dbReference type="InParanoid" id="C5DEY0"/>
    <dbReference type="OMA" id="ANFYHSG"/>
    <dbReference type="OrthoDB" id="427795at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050459">
        <property type="entry name" value="WD_repeat_RBAP46/RBAP48/MSI1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22850">
        <property type="entry name" value="WD40 REPEAT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="273"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR22735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="449" mass="49790" checksum="2AC998776F3D1784" modified="2009-07-28" version="1">MSEAKGNKIKNEEFKIWKKSVPSLYQHITTIKPSYTRLFEGAERIPKVVAFTHKIVPDREKGTFTTSLLCSQGSDIYEVDFQLPLGLHSNSEALPDPQYSSAFAEIVHSEKHEPKWTYQGNTIIKLEYVGGAEADAVAMTSTGSLAWFKDGCKVPVYVMEELVGPATSFSRIHNQGSTNNWIAADFAVSENTETVVKSQQSMATGPGEEHSLLKIVDNSQSPGNLIRTISIPGVSVTQSIKFHDNNMFSTCSDDNGIRFYDIRGDGKPLWTVFEPHDGKLTCYDVSPTLETLFITGSDTGVLKLWDLRSVIACEIEQKEPTEIAKLYHSGADPVADVRFGISSPTEVLSVGDSGNVYHWNLEPIFLREGDEDDEMAESEELQQQCLEFMHTGGGRRSLGQSRKRETVAWHPIVQGVVGCVDPDGLLTVYRGHYGATDKARAQDEREDNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEZ5</accession>
    <name>C5DEZ5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0D11022p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0D11022g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553188.1">
        <property type="nucleotide sequence ID" value="XM_002553142.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEZ5"/>
    <dbReference type="STRING" id="559295.C5DEZ5"/>
    <dbReference type="GeneID" id="8295428"/>
    <dbReference type="KEGG" id="lth:KLTH0D11022g"/>
    <dbReference type="eggNOG" id="KOG2406">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040069_0_0_1"/>
    <dbReference type="InParanoid" id="C5DEZ5"/>
    <dbReference type="OMA" id="DYRVWIH"/>
    <dbReference type="OrthoDB" id="27237at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010770">
        <property type="entry name" value="Ecd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13060:SF0">
        <property type="entry name" value="PROTEIN ECDYSONELESS HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13060">
        <property type="entry name" value="SGT1 PROTEIN HSGT1 SUPPRESSOR OF GCR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07093">
        <property type="entry name" value="SGT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="406"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="464"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="489"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="533" mass="61182" checksum="8E2772B71004A7AC" modified="2009-07-28" version="1">MIFFKLISSPYQLINKAEKMHESESEHLGKAALLWSDLSETHDGLDETPEETYIYFGIFHLDIIHGQSAEHLNRALKQFQDLIRPWNEFYPWTNYNGVKTEVVEDSASAPYILGSLCITDNLEEESLVVSLLQRFSSLQGPEIFVKVTGSDGEVLLIESHDVLPSEYEYPKGNNRLWIHEGKFKLIPSNVTPGRGMERSEALNFLLNTYYKLIEVPEVSSILHQRFVLDTPEKYLRNLYQLPLELNNKQHFELLKKKPRLIGYIIKCLYQENLSHEPEKIIEECENQPLTLLLPVSQCKLLSFYLQYSGIDLQSERLPSITGAIISRALEQVIRYDKHFQRENFTSCSEALDSFQVQLVSQGYLKKEVPHQKANMGALLIEAEAEVAEEDTMERMQSLFTGIKSSLEEEDNNKQANESSDDSDTAADHEAQKFFSAQNIDIDEDDFFEFFLTEALKVKKEDLKNYQSGSAPPSERYHEKEDEAEAVNEFEGHLSTDSRGHSNDTEALEELIEAMRLDPSAFENIQNILDLDSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DF51</accession>
    <name>C5DF51_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sidoreflexin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0D12254g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the sideroflexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553244.1">
        <property type="nucleotide sequence ID" value="XM_002553198.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DF51"/>
    <dbReference type="FunCoup" id="C5DF51">
        <property type="interactions" value="300"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DF51"/>
    <dbReference type="GeneID" id="8295484"/>
    <dbReference type="KEGG" id="lth:KLTH0D12254g"/>
    <dbReference type="eggNOG" id="KOG3767">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039425_0_0_1"/>
    <dbReference type="InParanoid" id="C5DF51"/>
    <dbReference type="OMA" id="GRVRHCA"/>
    <dbReference type="OrthoDB" id="6608471at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015075">
        <property type="term" value="F:monoatomic ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990542">
        <property type="term" value="P:mitochondrial transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004686">
        <property type="entry name" value="Mtc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00798">
        <property type="entry name" value="mtc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11153">
        <property type="entry name" value="SIDEROFLEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11153:SF6">
        <property type="entry name" value="SIDEROFLEXIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03820">
        <property type="entry name" value="SFXNs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="179"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="271"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR22806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="327" mass="35501" checksum="657E95D9B9952750" modified="2009-07-28" version="1">MASSVPGPLPLPDSRFDLSTYWGRVRHCSEIADPSMLLTTENDLQKARDVISSYRRGELKQTTPEFWHAKKQLDSTVHPDTGETVLLPFRMSCCVLSNLVVTAGMLTPGLGTAGTIFWQWTNQSLNVAINSANANKSHPMSMQQLITNYTMAVTASCGVAVGLNKIVPRLTKISANTRLILGRLVPFAAVVSAGIVNVFLMRGDEIRKGISVYDKNGDEVGKSKKAAFLAVGETALSRVINATPIMVIPPLLLVRLQRNVLKGKSLGIQTLANLGLILGTSVAALPFALAVFPQYQDIDVHKLEKDLAGKKDKDGETVDKVYFNRGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFC8</accession>
    <name>C5DFC8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="15">KLTH0D14080p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0D14080g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SMC family. SMC6 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553321.1">
        <property type="nucleotide sequence ID" value="XM_002553275.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFC8"/>
    <dbReference type="FunCoup" id="C5DFC8">
        <property type="interactions" value="899"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFC8"/>
    <dbReference type="GeneID" id="8295565"/>
    <dbReference type="KEGG" id="lth:KLTH0D14080g"/>
    <dbReference type="eggNOG" id="KOG0250">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009063_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFC8"/>
    <dbReference type="OMA" id="FMCHRSL"/>
    <dbReference type="OrthoDB" id="10265785at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030915">
        <property type="term" value="C:Smc5-Smc6 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038729">
        <property type="entry name" value="Rad50/SbcC_AAA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19306">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES 5,6 SMC5, SMC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19306:SF6">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13476">
        <property type="entry name" value="AAA_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad50/SbcC-type AAA" evidence="14">
        <location>
            <begin position="71"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="27"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="236"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="387"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="693"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="769"/>
            <end position="852"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF13476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR22883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1102" mass="125021" checksum="41AE8DF5EC6BDDE4" modified="2009-07-28" version="1">MDINDNLVSASLVAMTGNVATAIGSSAILHDGEQRPSKRQRRFAPMTQHDSQTGAAGSEGANLNPPGFIKRVQLRNFMCHEHFELELGPRLNFIVGNNGSGKSAVLTAITIGLGAKAADTNRGSSLKDLIREGCNSSKIVVVLNNEGFGGYEQGTYGTEIRIERTIKKSGPAGFSLKSETGKEISNKKRDLQAIVDYFAVPVMNPMCFLSQDAARSFLTATTPADKYKHFMRGTLLEDTETNLKKALEIATTAQSNLNYHAENVKLLRQDYDHTKKLLKELTANHDLTDRKRALQGKLLWLSVVENETSQRKLEVAEAHYLAKIKESEGKSASRTNSKEQLKLDQAAAEQQLEEALSSWQTKKRLADEAQSLMHSARMEYNMQKEHREETQKHINDAEGKVRQFENTIKDFEERLEKQMGGDKKRMHEEIEDLKERIKTQNEFYSSMNIRLQELRNKEEMVTSNALPKIGSLQKSIHEKTEQIRDIAQGKRNFLSNFDPHMEKLVRLIEQRKSEFSSKPVGPLGNYVTVKSQFQEWARPIQRYLGGTLSAFVVANVSDGALLKSLIRACQIRSNINVITYKLNEFDFDSGKARSEYPSVADALEFKSRSLQCLFVDQNRIEKVVLVTNKSDARDVLKRHNKNVIMTLSLRDNRSGYQSSLSAHGGFRIDTIEYQDKMKLTTKVSDTAFLTEIVSEEKKELANLTSQRNDHLKKIGTEITTLREEMANTKSKISEFSKREAALRLDLERELDTSAIEQAESDKQVYVNALASYNLALDEVNEKIDIIRQKVQPLKVRYEQANNELNSAACALDDLKHIISKGSSRIEKLQDDIGFSEKKVEKYQQKRTELLQKINEFSDGIGTQISNAETYCTRAVAFAGDMPETREDTKAEIDRITHRIQLAENRVGLSQDQIMSLFESAKLKYKDAEQKFANVDKAVFQLNESLKRRWQSLTYAKTDTCVTADTDFKESLRFRNFSGGLNFNFSKETLNMLVKTPNDEKPRNVDTFSGGEKSFSQISLLLATWRPMRSRIIALDEFDVFMDQVNRQIGTKLIMNKLSKESRTQTVIITPQDIGKIANFEDPGIRIHRMKDPERLNNSSYYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFD0</accession>
    <name>C5DFD0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0D14124p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0D14124g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RAD52 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553323.1">
        <property type="nucleotide sequence ID" value="XM_002553277.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFD0"/>
    <dbReference type="FunCoup" id="C5DFD0">
        <property type="interactions" value="136"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFD0"/>
    <dbReference type="GeneID" id="8295567"/>
    <dbReference type="KEGG" id="lth:KLTH0D14124g"/>
    <dbReference type="eggNOG" id="KOG4141">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091426_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFD0"/>
    <dbReference type="OMA" id="WSVQRIG"/>
    <dbReference type="OrthoDB" id="206565at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045002">
        <property type="term" value="P:double-strand break repair via single-strand annealing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006312">
        <property type="term" value="P:mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.80">
        <property type="entry name" value="DNA repair protein Rad52/59/22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041247">
        <property type="entry name" value="Rad52_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007232">
        <property type="entry name" value="Rad52_Rad59_Rad22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042525">
        <property type="entry name" value="Rad52_Rad59_Rad22_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12132">
        <property type="entry name" value="DNA REPAIR AND RECOMBINATION PROTEIN RAD52, RAD59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12132:SF2">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04098">
        <property type="entry name" value="Rad52_Rad22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR22885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="213" mass="23769" checksum="7B50CC1A51BDF5F4" modified="2009-07-28" version="1">MSAYLTNLSYENAVYATGAGVSDSDVQDLQIVENWLNRPASEWAVQKIGVFQSKIEQYSYRIYHSSRYGKHPIAKLIPAFTLIQYANEALGYDGWSLEVTNVNVTSCREIGGEARAVNSGESTVELKYEVLTEAHVKVVLKDGTYTQSEGFGKAVALSKSDCFNKSKKEAINNALKKSFLKFETMVLDHEKKVASNYYVDGLYGSKQWSKQSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFL4</accession>
    <name>C5DFL4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0D16082p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0D16082g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553407.1">
        <property type="nucleotide sequence ID" value="XM_002553361.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFL4"/>
    <dbReference type="FunCoup" id="C5DFL4">
        <property type="interactions" value="626"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFL4"/>
    <dbReference type="GeneID" id="8295654"/>
    <dbReference type="KEGG" id="lth:KLTH0D16082g"/>
    <dbReference type="eggNOG" id="KOG3460">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076902_5_1_1"/>
    <dbReference type="InParanoid" id="C5DFL4"/>
    <dbReference type="OMA" id="FDSHCNI"/>
    <dbReference type="OrthoDB" id="29543at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040002">
        <property type="entry name" value="Sm-like_LSM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13110">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sm" evidence="1">
        <location>
            <begin position="3"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="81" mass="8905" checksum="8CD5282F10CBE3DC" modified="2009-07-28" version="1">MDTPLDLLKLNLDEKVFVKLRGAREMTGVLQAFDSHCNIVLSNATETIYELVDGELKSSTKGSEMVFVRGDSVTLITTPSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFQ7</accession>
    <name>C5DFQ7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 7">5-formyltetrahydrofolate cyclo-ligase</fullName>
            <ecNumber evidence="5 7">6.3.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0D17116g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4 7">
            <text>(6S)-5-formyl-5,6,7,8-tetrahydrofolate + ATP = (6R)-5,10-methenyltetrahydrofolate + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10488"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:57457"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the 5-formyltetrahydrofolate cyclo-ligase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.3.2" evidence="5 7"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR23012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553450.1">
        <property type="nucleotide sequence ID" value="XM_002553404.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFQ7"/>
    <dbReference type="FunCoup" id="C5DFQ7">
        <property type="interactions" value="230"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFQ7"/>
    <dbReference type="GeneID" id="8295699"/>
    <dbReference type="KEGG" id="lth:KLTH0D17116g"/>
    <dbReference type="eggNOG" id="KOG3093">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066245_2_1_1"/>
    <dbReference type="InParanoid" id="C5DFQ7"/>
    <dbReference type="OMA" id="STIYPCQ"/>
    <dbReference type="OrthoDB" id="2015992at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030272">
        <property type="term" value="F:5-formyltetrahydrofolate cyclo-ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009396">
        <property type="term" value="P:folic acid-containing compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10420">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002698">
        <property type="entry name" value="FTHF_cligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024185">
        <property type="entry name" value="FTHF_cligase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02727">
        <property type="entry name" value="MTHFS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23407:SF1">
        <property type="entry name" value="5-FORMYLTETRAHYDROFOLATE CYCLO-LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23407">
        <property type="entry name" value="ATPASE INHIBITOR/5-FORMYLTETRAHYDROFOLATE CYCLO-LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01812">
        <property type="entry name" value="5-FTHF_cyc-lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006806">
        <property type="entry name" value="FTHF_cligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="5"/>
            <end position="9"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="152"/>
            <end position="160"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00036539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR006806-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR23012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="213" mass="23814" checksum="C148950587306E00" modified="2009-07-28" version="1">MRVTKAAIRPGLKKLLREIPNASLELQSALILRALEPTLEDHTKVACYMDMERGEVRTMPIISHLFAVGKQVYLPRCTNTRISGHAVLRQGVESHPHLTFHQMLSLAQVQALKPQGKYQLREPALEEPHPLPPELDVVMVPGVAFSLANGARMGHGAGFYDDFIQRTLHTHGKRPLLIGLALKEQIVPEVPTEAHDHHMDCIICGDGSVHWFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFU2</accession>
    <name>C5DFU2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0D17952p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0D17952g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR23047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553485.1">
        <property type="nucleotide sequence ID" value="XM_002553439.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFU2"/>
    <dbReference type="FunCoup" id="C5DFU2">
        <property type="interactions" value="113"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFU2"/>
    <dbReference type="GeneID" id="8295737"/>
    <dbReference type="KEGG" id="lth:KLTH0D17952g"/>
    <dbReference type="eggNOG" id="ENOG502SC94">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_174950_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFU2"/>
    <dbReference type="OMA" id="KPMWPFY"/>
    <dbReference type="OrthoDB" id="5520611at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006995">
        <property type="entry name" value="ATP_synth_F0_jsu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28060">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT J, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28060:SF1">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT J, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04911">
        <property type="entry name" value="ATP-synt_J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR23047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="59" mass="6719" checksum="CC796F3AF6652184" modified="2009-07-28" version="1">MFKRFPTPILKPYWPFFVGGVFVYWGVGKAASASAQTAEFINDPRNPRFARGEKPVELK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DG02</accession>
    <name>C5DG02_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0D01342p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0D01342g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552782.1">
        <property type="nucleotide sequence ID" value="XM_002552736.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DG02"/>
    <dbReference type="FunCoup" id="C5DG02">
        <property type="interactions" value="63"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DG02"/>
    <dbReference type="GeneID" id="8295002"/>
    <dbReference type="KEGG" id="lth:KLTH0D01342g"/>
    <dbReference type="eggNOG" id="KOG0619">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_335290_0_0_1"/>
    <dbReference type="InParanoid" id="C5DG02"/>
    <dbReference type="OMA" id="YNDYFKR"/>
    <dbReference type="OrthoDB" id="1394818at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019487">
        <property type="entry name" value="RAM_signalling_pathway_SOG2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366">
        <property type="entry name" value="IG(IMMUNOGLOBULIN) AND LRR(LEUCINE RICH REPEAT) DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366:SF96">
        <property type="entry name" value="LEUCINE RICH REPEAT CONTAINING 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10428">
        <property type="entry name" value="SOG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="432"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="439"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR22344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="746" mass="83084" checksum="1220A432A1C8F0DE" modified="2009-07-28" version="1">MNCHEHTWSPYSSHQRNQAKGYVRTALSFGLMDGRGDILSNLRQNVDRRNNLAFLASKQVSQRDPTIKLIDLGITSLTQETIDLLLSVERLSLQKNLLTSLPEGFNKLAELRYLDLHDNKLQEIPATLMQCQRLEILDLSSNYITHLPHEYSRVWCENIKVLSLKNNSVSSIRELYPAIMSLRSLKILEIEGNQFPAEELEQIKSEVPPSSLPDSVSPDEYWCVALRKHFSDNKISKAAKRRGFINVSEEQHRQEELYSNNKYNDYFKRLSVLPEETTSQRVTQDELVVACRKLLFSFTECQQSIRKITSLCEDKAVAVNSVSLLYSVRSHIDNLVELLEQSESPTSAKNDVMVKLCLTIISIFKQIFALLRKNFKSFFQGNDICFARIFYMNILCSYTEMYNAWSQITPQDSIAPVKKRIARTHSLSVSQGFKHITPRSRSNTLQRSATGNSAHPTTSPIPQSSNLRSAPGSVMTTPPPSQSNLAGEVRAANDGSPSFKPDLTQQSPHAGRRAADVPPQAGTPAVPVVDNKMGPEDQGSADIDAQLYHTLNTVISMVTVVYSQLTSAITKSAIASTKTTEMSSITSSIAAKIKDLTDTCFQSMELSKLLKGRLSDVSLGKSEVFSTTAEKAKTWESINAFLKSIISILANTKIIMKDLPILNEVRPNLASLAKITKDVTVILDLSSYRSISQAAQQQSQETATVKQQSHPQASGMLEESHNPILVTPLSTPSLVTVHASNPFDQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>C5DG20</accession>
    <name>C5DG20_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0D01760p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0D01760g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
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            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
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    <dbReference type="eggNOG" id="KOG0421">
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        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0016740">
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    </dbReference>
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        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
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    </dbReference>
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        <property type="entry name" value="Ub_conjugating_enzyme"/>
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        <property type="entry name" value="UBQ-conjugating_AS"/>
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    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
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        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
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    </dbReference>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50127"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAR22362.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
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    </evidence>
    <sequence length="161" mass="18146" checksum="1BACDF1A3FE7ECA5" modified="2009-07-28" version="1">MEGSSKSSIPDGHSVVSRLQSELMQLMLSPSPGLSAFPEDEEDLTKWCGIITGPDGTPYEGMRFKIALEFAQTYPYTAPKVRFVSPMWHPNVDMSGNICLDILKDQWSAVYNVQTILLSLQSLLEEPNNSSPLNAVAAELWDKDMREYKKKLIARYEEIDD</sequence>
</entry>
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            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
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        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
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                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="CAR22453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002552845.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGB1"/>
    <dbReference type="FunCoup" id="C5DGB1">
        <property type="interactions" value="175"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGB1"/>
    <dbReference type="GeneID" id="8295116"/>
    <dbReference type="KEGG" id="lth:KLTH0D03872g"/>
    <dbReference type="eggNOG" id="ENOG502S75Y">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_885873_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGB1"/>
    <dbReference type="OMA" id="FESTGCY"/>
    <dbReference type="OrthoDB" id="4036600at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <end position="124"/>
        </location>
    </feature>
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            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
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            <end position="314"/>
        </location>
    </feature>
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            <end position="166"/>
        </location>
    </feature>
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        </location>
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        </location>
    </feature>
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    </feature>
    <evidence type="ECO:0000256" key="1">
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAR22453.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="314" mass="33442" checksum="EB6D996AD19394C1" modified="2009-07-28" version="1">MEVTTHAQHKAVTLYKEPHHLDSVNWEEGLGKGCGKSAQQDLDQLTVVDSIVVTPDAAPQSPQDYTRSGRPAVPHYTLSSSGSAMADKQHTFKAPSPARTPGRPTRPTLNVQSSSSSSLSSLRSRARRLSSEEIINEMENEQDAIVVRLLREIDQLKDENNRLRKNLCAVLNGDPQTPAGPALSRRSSLNSCASNSSGSGSLSSSHAGTTPGVLAATPTASSPALSRRPSSSATPIDTLTPTLLLQRKRNSLSSSVPATPKNSGDFVDLYAPAPVSKFGAVDVSLDAPGSCGYRRRRSSMKSTEGSNKLQRSTR</sequence>
</entry>
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    <accession>C5DGB2</accession>
    <name>C5DGB2_LACTC</name>
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        <submittedName>
            <fullName evidence="3">KLTH0D03894p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0D03894g</name>
    </gene>
    <organism evidence="3 4">
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        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
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        <lineage>
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            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
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                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
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                <person name="Jean G."/>
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                <person name="Martin T."/>
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                <person name="Rolland T."/>
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                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
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                <person name="Talla E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_002552846.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGB2"/>
    <dbReference type="STRING" id="559295.C5DGB2"/>
    <dbReference type="GeneID" id="8295117"/>
    <dbReference type="KEGG" id="lth:KLTH0D03894g"/>
    <dbReference type="HOGENOM" id="CLU_390818_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGB2"/>
    <dbReference type="OMA" id="HSAFATH"/>
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        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950468" evidence="2">
        <location>
            <begin position="23"/>
            <end position="706"/>
        </location>
    </feature>
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        </location>
    </feature>
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        </location>
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            <begin position="492"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="541"/>
            <end position="554"/>
        </location>
    </feature>
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        <location>
            <begin position="555"/>
            <end position="570"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR22454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="706" mass="69322" checksum="BEA0ABCAFDA62F23" modified="2009-07-28" version="1">MLCRPILLLFTAFGGFASCISALSTTTITTESITTSFVCGRCTTNQHETTLSSSSTTAVETSACHQCVTSQYESSLSSISTSVSSTSTIPTSPLSSTTISSTIVSTSYVCGKCTTNISFREVVVSSTSETVPSAKHSSSTPTSSTSASDESSSATPSSAMPSSATPSSAMPSSATPSSTTTASSTASSTTISTTTSSSSASSSSTISSFTISSTTPSSIASSNATPSIITISSDTSSSSTLSSASSSVARTSSGNVESSPSGPKTTTTLNKSTTTETESCDCTTYPGSSISTTTTLVSTISTSSSATTQSSTQSSTTSGTSSLSSEMSSSKSSASSVSLSSSSKVSSRTGFSSTETSRATTVSETQHSLSSESNTPTTLTSIPGSLSTSATFSSFSSTAFSSVSTSSPTAESVSSLVSSTPSVGSQTSSTLTSSTSFPGVPSSVTSSGNVPSSFSDIGTSSTSVLTTTYSTTNSGSTSITLTTDSSFRTLESSASSTPASSTPASSTPASSTPASSTTASSTTASSTTVTVSSSLPGLNMSTTLSSSTLLSTTSPGQPISTASTSTGPLTIPTASNSLPVLLVSTSCTAANDTNCAVPTTDSAPAPTSTSTPPSSSSSNASKSEEVPSSAASSSSPATVSGPTSSQSSVHTTSSTFASYAPKSFSFGSTSTQPFPTASSTASLLSSYTGGASKAVPRIAFAVLLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGI7</accession>
    <name>C5DGI7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">U3 small nucleolar RNA-associated protein 11</fullName>
            <shortName evidence="6">U3 snoRNA-associated protein 11</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0D05632g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">Involved in nucleolar processing of pre-18S ribosomal RNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the ribosomal small subunit (SSU) processome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 6">Nucleus</location>
            <location evidence="2 6">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the UTP11 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552967.1">
        <property type="nucleotide sequence ID" value="XM_002552921.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGI7"/>
    <dbReference type="FunCoup" id="C5DGI7">
        <property type="interactions" value="776"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGI7"/>
    <dbReference type="GeneID" id="8295195"/>
    <dbReference type="KEGG" id="lth:KLTH0D05632g"/>
    <dbReference type="eggNOG" id="KOG3237">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061887_0_2_1"/>
    <dbReference type="InParanoid" id="C5DGI7"/>
    <dbReference type="OMA" id="DLKYVVM"/>
    <dbReference type="OrthoDB" id="29058at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007144">
        <property type="entry name" value="SSU_processome_Utp11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12838">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12838:SF0">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03998">
        <property type="entry name" value="Utp11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015952">
        <property type="entry name" value="U3snoRNP11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="199"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR015952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR22529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="250" mass="29517" checksum="300D8A021F07BEA5" modified="2009-07-28" version="1">MAKLVHNVQKKQHRERSQVASRSRFGFLEKHKDYVKRAQDFHRKQATLKVLREKAQQRNPDEYYHAMHSRTLDSKGLLQKSRRAADEDASLSTDQVKLLKTQDANYVRTLRQNERQALERQSQNTMFKSNGSHTVFVDDAESMHAFDAHKHFNTTPDMLHRRENRLTKEQLADQGLQAGAKQAGLDPETLERKRLKKLRQLQQHQDRERQLSGVLQRMEMEREGMKKGSKKKIRDAHGNTAFKWKKQRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGQ2</accession>
    <name>C5DGQ2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0D07150p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0D07150g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553032.1">
        <property type="nucleotide sequence ID" value="XM_002552986.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGQ2"/>
    <dbReference type="FunCoup" id="C5DGQ2">
        <property type="interactions" value="788"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGQ2"/>
    <dbReference type="GeneID" id="8295263"/>
    <dbReference type="KEGG" id="lth:KLTH0D07150g"/>
    <dbReference type="eggNOG" id="KOG0804">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009969_0_1_1"/>
    <dbReference type="InParanoid" id="C5DGQ2"/>
    <dbReference type="OMA" id="RFNSIEP"/>
    <dbReference type="OrthoDB" id="273556at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16457">
        <property type="entry name" value="RING-H2_BRAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011422">
        <property type="entry name" value="BRAP2/ETP1_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047243">
        <property type="entry name" value="RING-H2_BRAP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24007">
        <property type="entry name" value="BRCA1-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24007:SF7">
        <property type="entry name" value="BRCA1-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07576">
        <property type="entry name" value="BRAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00290">
        <property type="entry name" value="ZnF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50271">
        <property type="entry name" value="ZF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="247"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="UBP-type" evidence="8">
        <location>
            <begin position="281"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="518"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="402"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="531"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR22594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="540" mass="61995" checksum="7D0D0F81B7782288" modified="2009-07-28" version="1">MSIVLGSAQVFIRPKKPCMSFRYRLVIELDVLNEAKKAHEFWIRDEQETTTESTTVRSDLEDSATEDWRHEDVKIGIMNAMSQRDNFIEAEFSSPGIVRLFKIGEEKPDAGTAGDSVLRIPGDDTMISVLFVPSYLSARDLLHSFLGDAVTSRVSHFRIIRSNKDAGYSFILLMKFRDPESAALFKEQYNGRNFNQMDPETCHVIRIKEVVFQERLFPKPQSAGSQFLMNDPFTGHKETGDVELPTCPVCLERLDSEVTGLATIPCQHTFHCQCLNKWKDSRCPVCRYSGLKVTKSSLLRQGVRCSTCGANENLWICLICGNVGCGRYNFKHAVQHFKDTAHFFSMDVATQRVWDYAGDNYVHRLVQNEVDGKLVEVGSSSSRDSSGKRDKEYHLEYVQVLLSQLESQREYYESKLRQLEINEEELKSVHSLQQQIQELKLQAVVTRSKSEHKVNEMRKQLEEERLLSRALQKNLDHLSNTIGDLQKQQEQSVIENQELKEQVTDLMFFLESQEKLKDADESVKQGTLVMKPKKNSRKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGT6</accession>
    <name>C5DGT6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein mL44</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0D08140g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ribonuclease III family. Mitochondrion-specific ribosomal protein mL44 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553066.1">
        <property type="nucleotide sequence ID" value="XM_002553020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGT6"/>
    <dbReference type="FunCoup" id="C5DGT6">
        <property type="interactions" value="503"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGT6"/>
    <dbReference type="GeneID" id="8295300"/>
    <dbReference type="KEGG" id="lth:KLTH0D08140g"/>
    <dbReference type="eggNOG" id="KOG3769">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034765_1_0_1"/>
    <dbReference type="InParanoid" id="C5DGT6"/>
    <dbReference type="OMA" id="YLYSPGN"/>
    <dbReference type="OrthoDB" id="67027at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19873">
        <property type="entry name" value="DSRM_MRPL3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="entry name" value="Ribonuclease III domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044443">
        <property type="entry name" value="Ribosomal_mL44_DSRM_fung"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044444">
        <property type="entry name" value="Ribosomal_mL44_DSRM_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11207:SF32">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11207">
        <property type="entry name" value="RIBONUCLEASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22892">
        <property type="entry name" value="DSRM_MRPL44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00535">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="RNase III domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50137">
        <property type="entry name" value="DS_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50142">
        <property type="entry name" value="RNASE_3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2 8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RNase III" evidence="10">
        <location>
            <begin position="123"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="9">
        <location>
            <begin position="286"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR22628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="374" mass="41829" checksum="DB7DBBAF02FB922F" modified="2009-07-28" version="1">MSQQLLLSGRLARSLHVVARSKRVWLRGFPAPQRSIHLSPRQRQQQVKLETPELNKYREYYKRLQAAVNDVPEELASKSPSLVTLHRRLNLPESFQLSTLARCLTCRSSRLPSDLNVPSSGAAFTSSVPTSNFYDNHGLNIFGKNLLTYHVTRQTMAKYPRLPTVVLNAAVDAYISQSVLASVGRSWGIELENSSVLDRYLREEPIQVSLGKLRFFNNALKREDGVELITSHNFSEDAAYALAVRSVIGALWAYTHETDPTFAFRFIDDHILSRKLDVTRIFQFEQPARELAVLCRRENLSPPVSKLLAESGRLSKAPVFIVGVFSGAEKLGEGFGSSLKEAKARAATDALMKWYCYEPVQGQAPVIDPGAVIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>C5DH70</accession>
    <name>C5DH70_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0E01826p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0E01826g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="5">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553568.1">
        <property type="nucleotide sequence ID" value="XM_002553522.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DH70"/>
    <dbReference type="FunCoup" id="C5DH70">
        <property type="interactions" value="1101"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DH70"/>
    <dbReference type="MEROPS" id="T01.A12"/>
    <dbReference type="GeneID" id="8291707"/>
    <dbReference type="KEGG" id="lth:KLTH0E01826g"/>
    <dbReference type="eggNOG" id="KOG0179">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035750_1_0_1"/>
    <dbReference type="InParanoid" id="C5DH70"/>
    <dbReference type="OMA" id="CSGCWCD"/>
    <dbReference type="OrthoDB" id="268479at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010499">
        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03757">
        <property type="entry name" value="proteasome_beta_type_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000027">
        <property type="entry name" value="Proteasome subunit beta type-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF2">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA TYPE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0647">Proteasome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00026071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR23131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="242" mass="27248" checksum="3F3AC0ACC5766AE4" modified="2009-07-28" version="1">MATTVASEYSNEVKNTPIEHQFNPYSDNGGTILGIAGEDFAVLAGDTRHTTDYSINSRYEPKVFDCGDNILISANGFAADGNALVKRFQNSLKWYHFDHNDKKLSMRSAARNIQHLLYGKRFFPYYVHTIIAGLDEEGKGAVYSFDPVGSYEREQCRAGGAAASLIMPFLDNQVNFKNQYEPDSHGTKRKHVKFLTVEEVVQLVRDAFSSATERHIHVGDGVEILIVTKEGVRKEFFELKRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>C5DH78</accession>
    <name>C5DH78_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phospholipid scramblase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0E02002g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the phospholipid scramblase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553576.1">
        <property type="nucleotide sequence ID" value="XM_002553530.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DH78"/>
    <dbReference type="FunCoup" id="C5DH78">
        <property type="interactions" value="195"/>
    </dbReference>
    <dbReference type="GeneID" id="8291715"/>
    <dbReference type="KEGG" id="lth:KLTH0E02002g"/>
    <dbReference type="eggNOG" id="KOG0621">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023808_0_1_1"/>
    <dbReference type="InParanoid" id="C5DH78"/>
    <dbReference type="OMA" id="QNWHLWR"/>
    <dbReference type="OrthoDB" id="191150at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017128">
        <property type="term" value="F:phospholipid scramblase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005552">
        <property type="entry name" value="Scramblase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23248:SF9">
        <property type="entry name" value="PHOSPHOLIPID SCRAMBLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23248">
        <property type="entry name" value="PHOSPHOLIPID SCRAMBLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03803">
        <property type="entry name" value="Scramblase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR23139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="328" mass="37133" checksum="3A3ECA6FC3F6EB59" modified="2009-07-28" version="1">MFRRAFNRRWYSPLPRVVRHRPVPRAGRTTNAIYDATPSRGANELGSTRSIIQPHHPVATTILNEPTVVIERQIEMMNVFLGFEQANKYVIMDALGNRIGYMQERDFSIAKAVMRQFYRLHRPFVVDVFDNWGNLLLTIKRPFSWINSHIKAILPDDASPQQSLGSSSVDVAPFGSGPVPKSTSTFGEGGILVGESVQNWHLWRRRYELFEREAPTEDSFSQFAEIDAPFLSFEFALKDEVGKTMGGVDRNWVGLGRELFTDTGVYIVRMDSQQSLQGVLPAEIISDRILNLDQRAVLLANAVSIDFDYFSRHSRHGGGFIGFGDYGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHF0</accession>
    <name>C5DHF0_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ordered locus">KLTH0E03828g</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:17990"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:46609"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553648.1">
        <property type="nucleotide sequence ID" value="XM_002553602.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHF0"/>
    <dbReference type="FunCoup" id="C5DHF0">
        <property type="interactions" value="165"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHF0"/>
    <dbReference type="DNASU" id="8291797"/>
    <dbReference type="GeneID" id="8291797"/>
    <dbReference type="KEGG" id="lth:KLTH0E03828g"/>
    <dbReference type="eggNOG" id="KOG1027">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004875_2_1_1"/>
    <dbReference type="InParanoid" id="C5DHF0"/>
    <dbReference type="OMA" id="IRYYCSE"/>
    <dbReference type="OrthoDB" id="63989at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990604">
        <property type="term" value="C:IRE1-TRAF2-ASK1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070059">
        <property type="term" value="P:intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036498">
        <property type="term" value="P:IRE1-mediated unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09769">
        <property type="entry name" value="Luminal_IRE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10422">
        <property type="entry name" value="RNase_Ire1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000077">
        <property type="entry name" value="Putative Serine/threonine-protein kinase/endoribonuclease IRE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000572">
        <property type="entry name" value="Serine/threonine-protein kinase/endoribonuclease IRE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.180">
        <property type="entry name" value="KEN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045133">
        <property type="entry name" value="IRE1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010513">
        <property type="entry name" value="KEN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038357">
        <property type="entry name" value="KEN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_b-propeller_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13954">
        <property type="entry name" value="IRE1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13954:SF6">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06479">
        <property type="entry name" value="Ribonuc_2-5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00580">
        <property type="entry name" value="PUG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51392">
        <property type="entry name" value="KEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8 21" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950479" description="non-specific serine/threonine protein kinase" evidence="21">
        <location>
            <begin position="27"/>
            <end position="1118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="550"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="681"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="domain" description="KEN" evidence="23">
        <location>
            <begin position="988"/>
            <end position="1118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="72"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="605"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="86"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="605"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="631"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="19">
        <location>
            <begin position="641"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="660"/>
            <end position="669"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="CAR23211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1118" mass="126267" checksum="37BF7CF21B037060" modified="2009-07-28" version="1">MRRPRGIDLLAIFVWGLLLLIPAVSGSWLRWRGRREHTKESLAPLISEAQDAPISSLHNEVHRAKPTVFKKSLSVDTSSPAPAQLGERRPTDDSSKTKFTSNSLLRNVHGFQDSPPPKNGVSVPFNHRNSEGLKILDYIIVTDLEGGLHAVHRKTGEILWSNTNVSAPSIYVSEPTDSSNETLIVEPYGDGNIYYFNIYQGLQKLPISIKHLVNASPLDLKTRIVVDENGTTIDDEKIYTGSRHSAVFNINIKTGQVLSAYGPGTENKAYDDLGTNCTEQSFSTGACEDVFVLGRTTYELSIYSKLGTVYNVTYGAWQQNSADNSLANQYDRSADGILIAPFGEKSLLAVDSELNFAKWISSQFSGTINNVFDVFLDGKTSEKIILPHPMNPRTVSNHQSRVLLGRTSEGCWYAMSDEFYPSLVDSSSISIYNHRREWKMPQTLKDVQSLQDAMVGVHDLQSLQFEQIIEHEPRPSPKSLPASSMLLIDSPRPPRSDNRGLIVEEDSTKALEKYISKEEMLALKLQAKEKMAKDLLSAHQKSFAYRFANFVYRIVEGGLMLLFSFFILGLLSRFKVLAPLHVFLERSGLISGQNLETGSIKIEDKEDTSNTQDEPHEKHVQILNPTSDNEQNSKEEGSSERRKRKRGSRGKKKNKKVHEFSQTDATGSSEADVEADLKHLTVSKKVLGYGSSGTVVFQGTFQHRPVAVKRMLIDFYDIATQEIKLLTESDHHPNVVRYYCSEITGRFLYIALELCTSTLEDVVEGKKESSKIIEAREHLDPINVLFQIAQGVAHLHSMKIVHRDLKPQNILVAPTRKYMQHLDSSLAPMRVLISDFGLCKRLEPDQSSFHTKQGNASGTSGWRAPELLDGCATSDTENDGSYGSAESSISYVYDPFYHKRLTRAIDIFSMGCVFYYVLSKGSHPFGDKYSRDSNILKESWCLDDINKSLKDRCSVIEAKDLIRQMISNNPSQRPAASQVIKHPLFWPISKKLEFLLKVSDRFEVERRVPPSPLLLKLEEASPRVIPNRDWTVKFDGVFMENLGKYRKYSGEKLMDLLRAFRNKYHHFMDLPPELAEVMGPIPDGFFLYFARRFPNLLLEIYFVVQRNLKHDQILGDYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHK2</accession>
    <name>C5DHK2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0E04972p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0E04972g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553700.1">
        <property type="nucleotide sequence ID" value="XM_002553654.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHK2"/>
    <dbReference type="FunCoup" id="C5DHK2">
        <property type="interactions" value="366"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHK2"/>
    <dbReference type="GeneID" id="8291849"/>
    <dbReference type="KEGG" id="lth:KLTH0E04972g"/>
    <dbReference type="eggNOG" id="KOG3487">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085828_0_0_1"/>
    <dbReference type="InParanoid" id="C5DHK2"/>
    <dbReference type="OMA" id="RYMNQFI"/>
    <dbReference type="OrthoDB" id="10252102at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14825">
        <property type="entry name" value="TRAPPC2_sedlin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006722">
        <property type="entry name" value="Sedlin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12403">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04628">
        <property type="entry name" value="Sedlin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR23263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="170" mass="19147" checksum="44DD4E7549642131" modified="2009-07-28" version="1">MPCYFAIIGARDNPIYEAEFTSQQLNSFPPELKELNPFILHASLDIIEDLQWQVNPQSINSGGGSGGFLRSRHSNNNGNCYLGKIDHFYGLAITGYLSYGNMKFVMIHSSNGNGSTPAQVEDGSVKNFYQEVHELFVKTLMNPFYKLNDPITSPVFDSKVRTLARRYLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHL7</accession>
    <name>C5DHL7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0E05390p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0E05390g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S54 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553715.1">
        <property type="nucleotide sequence ID" value="XM_002553669.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHL7"/>
    <dbReference type="FunCoup" id="C5DHL7">
        <property type="interactions" value="672"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHL7"/>
    <dbReference type="MEROPS" id="S54.007"/>
    <dbReference type="GeneID" id="8291868"/>
    <dbReference type="KEGG" id="lth:KLTH0E05390g"/>
    <dbReference type="eggNOG" id="KOG2980">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034022_2_1_1"/>
    <dbReference type="InParanoid" id="C5DHL7"/>
    <dbReference type="OMA" id="LWYPRIA"/>
    <dbReference type="OrthoDB" id="10260614at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1540.10:FF:000012">
        <property type="entry name" value="Rhomboid family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050925">
        <property type="entry name" value="Rhomboid_protease_S54"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43731:SF14">
        <property type="entry name" value="PRESENILIN-ASSOCIATED RHOMBOID-LIKE PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43731">
        <property type="entry name" value="RHOMBOID PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="134"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="193"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="235"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="266"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="299"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S54 rhomboid" evidence="8">
        <location>
            <begin position="172"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR23278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="334" mass="37413" checksum="386EC9D8244E5F46" modified="2009-07-28" version="1">MNSFVSRFGKHFFLSNAINIQTKPLSASILGPLGLNLRQRIPTQPARFFSRTSEIKSSWRDVFNGAGASRYARLNRFQQFEKGSRNTNNPFSNNSLRNVTIAGALFMGGTYFGLPYLFDNVPPFTYFRAHPSSLVYGLIGLNCGVFALWRVPQCWRFLQRYMLLEKDHIYSKWSLIGSAFSHQEVWHLGMNMLALWSFGTTVATMLGPANFMSLYLNSALAGSLLSLWYPRIARISMMAPSLGASGALFGVFGCFSYLIPHAKIMLFVFPIPGGAWLAFLGSMVWNAAGCALRWGSFDYAAHLGGSVAGIVYAYLISEQLKKEKARRMQRFSVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHN7</accession>
    <name>C5DHN7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0E05830p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0E05830g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553735.1">
        <property type="nucleotide sequence ID" value="XM_002553689.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DHN7">
        <property type="interactions" value="1059"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHN7"/>
    <dbReference type="GeneID" id="8291888"/>
    <dbReference type="KEGG" id="lth:KLTH0E05830g"/>
    <dbReference type="eggNOG" id="KOG0915">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000880_1_1_1"/>
    <dbReference type="InParanoid" id="C5DHN7"/>
    <dbReference type="OMA" id="CRIKDIE"/>
    <dbReference type="OrthoDB" id="16066at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024372">
        <property type="entry name" value="Ecm29_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055443">
        <property type="entry name" value="HEAT_ECM29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23346:SF19">
        <property type="entry name" value="PROTEASOME ADAPTER AND SCAFFOLD PROTEIN ECM29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23346">
        <property type="entry name" value="TRANSLATIONAL ACTIVATOR GCN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13001">
        <property type="entry name" value="ECM29_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24492">
        <property type="entry name" value="HEAT_ECM29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0647">Proteasome</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Proteasome component Ecm29 N-terminal" evidence="6">
        <location>
            <begin position="16"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="domain" description="Proteasome adapter and scaffold protein ECM29 HEAT-repeat" evidence="7">
        <location>
            <begin position="1299"/>
            <end position="1459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1675"/>
            <end position="1699"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF24492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR23298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1851" mass="207305" checksum="7DB7626A8387CD32" modified="2009-07-28" version="1">MKGPMMLSESQELQLIEKVDLRFVLASSEDALERSLDTFFPALLLKLDSPHGSARVAVFNSIKNVMGRISSLTSVKLPVKKLIVQAKTWKPSGDHSENSVGLYSLLFASKGVDRLSPEEKTELIPEGIKGISALPVSLKPRMFHILCKLLLSWKAPTESSEDSAKTFLTIGSALDRSYLFQKFRQFFFLNPLKPELGSAAIPRGYSCPGLNVQEVEFFTFSAGVSFTRDSLSTYKRAIFTFMTRAFALEDRALLEFLVVVSADSTDLSDQAIALVKKYQVPYEDQQFVDTLIGLYIGERLTNRPPVGRELQEVILSILIKSVCATKSPGKVSLICSMGLNSTHYKLRSLSLTFVQHVARHNYEALTFQDTQSDYSVNIASLIRNNLHGEGWPQYQLNSSVPNFNIGLEQRRRQYETLGVVLKQDPQLLMDFSFIEFLFDSLKSDLREFRKSIQETLNSLCVHVACLPEASKFKLRTLAKKLLADNFDVVYGDKEAIDALMYCRAVAIKFVNSAFPFDDSEARLLNVMGLFRENRHDIREESTKGLHPYWFKMTQCINRSKTGVLEDFKSPSTNEVIFPDVRSLMDLFQSQMEHSTDVKTSALFQSFDYGANFVFQTLISQAIVGKKSSVLQDEFWHLRVAHAIQSDSNVRSLVESEVLSLDSNVLSTFTHMLLSHFVGRESRSENNSVNNETFSMGFVSLFFASYMNHSMLQKMSDFLPKLFELLHRYLLLSEADIECAAKIYGIIQSSNFSDGTASRMAEATRVPIESGDFVPAILTSSFTLPLLGLQEKALTAEEALPLIDKVIVSLEVTDLKPNGLRCLDPILKFGLLRLLSKEKRKATVQRVMIALRRYPLRTQPTIQAWSQLSLYAAEFGFIDTFFDALEESHLSKEVDVLFFAGEGMTVLAGGWNSSILRDKEYLKKNELDFLTNTFGDTSSELIIGKLLVLCEASKPSLRKAACIWLLCFVQYLGHTDSVRTHARKIHVSFARFLADNDDLVQDSASRGLGLIYDVSDKDLQEEMLKGLFKFFIDPKAALDMSSGTISSESQLFEPGTMNTGEGSISTYKDLLSLASEAGDPSLVYKFLPLARNTALWSSRKGVAFGLTSIFSKSSVDEFLRSTPHASQKLIPLLYRYRFDPFKNVSEAMNGIWNALVDNTSATITEYFDEILNSILQGIGGKDWRVREASTTALIDLLEITNEKQYQDKLEEIWTMAFRAMDDIKESVRSAGIKVTKILSKILVRSVKHDDINGKSSQPILEKLIPFFLGHKGLNSDAEEIRAFSLETLLVLIKEASSAVRSFAPSLIYEFLLLLSPLEPQVVNYLTMNAEKYNLNTSDVNAQRALGVQQSPIMRSIETLVGNCDTGLLPSVVDKVIKASKKSVGLPSKIGSSKVISLLVLQNPTSLSEFGSKLLKTCFSGLNDRSFTVSEAYTVSFGRVCKVCKPGKVGKYAGKLTEKYFNSEDSSSRVIAGMAIMSMFKYSTEMFQNVESALLPLVFIGMHDSDDVAETYTKIWNESTSSSSGTVNLYLEEIISLAAQRLKSNNFNIKITCAKSVCEACAQASVETPLNTIPQLFKVILETTQGRAWQGKETVVETLVVLARKFKPFYLNSTAIRSSVESRLINEISRTNFGYVRDVIFTSLSYSADFWSPALHEKNLEVAMHIIERIDNGADETASDENLDESNYKKQRVSTDVNRKSRKDNVASEEYKIKLLKKVVLLYRMSDDGDLSQRTLEAITKASLNLFESSNIIFTWRSQIAVCELGSAILERAPQGLTEGEQTCLKELWTKSFAQASSPESIENVKIQCVRFGKSLLEKKVNFWFIVENDLRRLMHLDPSPVVSVELKNAGVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>C5DI06</accession>
    <name>C5DI06_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0E08690p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0E08690g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553854.1">
        <property type="nucleotide sequence ID" value="XM_002553808.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DI06"/>
    <dbReference type="FunCoup" id="C5DI06">
        <property type="interactions" value="55"/>
    </dbReference>
    <dbReference type="GeneID" id="8292015"/>
    <dbReference type="KEGG" id="lth:KLTH0E08690g"/>
    <dbReference type="eggNOG" id="ENOG502RXX6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084558_1_0_1"/>
    <dbReference type="InParanoid" id="C5DI06"/>
    <dbReference type="OMA" id="TIGWAPL"/>
    <dbReference type="OrthoDB" id="2103474at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025187">
        <property type="entry name" value="DUF4112"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35519">
        <property type="entry name" value="MEMBRANE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35519:SF1">
        <property type="entry name" value="YALI0C06193P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13430">
        <property type="entry name" value="DUF4112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="81"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="132"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="185"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="227"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR23417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="250" mass="27965" checksum="359B9C1AF13B7BF2" modified="2009-07-28" version="1">MSSWVQGFVQGLAQDYATDKWNGYAEVKFQPTRDPFYEKTANGKTRRRKLPDYCSQREKKLWKKLQNRAWKDDRCLCGCLWVNWGLGLAPVLSIIPTIGPIIMYMVHSKLISMADKELSLPADMIAKLHGNIMLDLLISLPPLLGTLLAWLNACSTRNCALIYNYMAKNATKKHNAMVQQEQLYTSPAGVPPPGPAAYQKTNAYRPEYQGNRPAPPPPATRAVSPGARTQQAAQMPPQQISSRPAPRAAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>C5DI68</accession>
    <name>C5DI68_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0E10120p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0E10120g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553916.1">
        <property type="nucleotide sequence ID" value="XM_002553870.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DI68"/>
    <dbReference type="FunCoup" id="C5DI68">
        <property type="interactions" value="1162"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DI68"/>
    <dbReference type="GeneID" id="8292080"/>
    <dbReference type="KEGG" id="lth:KLTH0E10120g"/>
    <dbReference type="eggNOG" id="KOG0319">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009276_0_0_1"/>
    <dbReference type="InParanoid" id="C5DI68"/>
    <dbReference type="OMA" id="PYVQRHF"/>
    <dbReference type="OrthoDB" id="5414888at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057644">
        <property type="entry name" value="Beta-prop_WDR75_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013934">
        <property type="entry name" value="Utp13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19854">
        <property type="entry name" value="TRANSDUCIN BETA-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19854:SF15">
        <property type="entry name" value="TRANSDUCIN BETA-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23769">
        <property type="entry name" value="Beta-prop_WDR75_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08625">
        <property type="entry name" value="Utp13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="4 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="139"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="399"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="WD repeat-containing protein 75 second beta-propeller" evidence="9">
        <location>
            <begin position="413"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="503"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="545"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="587"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="629"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="domain" description="U3 small nucleolar RNA-associated protein 13 C-terminal" evidence="8">
        <location>
            <begin position="682"/>
            <end position="822"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR23479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="826" mass="91728" checksum="38BE0FE12E29982F" modified="2009-07-28" version="1">MDLKSSYSNSELRPFYAGSGAVASVSEDGRLLATSVIDEVNIIELAPTKRVLHRIENDDEQEITALKLTPDGEYLCYVSQAQLLRIYHLRSKRVVRSMKVSSPVYVIDCDPSSTLVALGGTDGSVSVIDIENGYVTHSLKGHGATISSVKFFGELNSTTWRLGSGDTNGVVKVWDLVRRSCLHTIQEHAAAVRGLDFRVSDADDDVLELLSGGRDDIVNLYQLGSTKKVKLVKTIPVHQQIEACGFIHDGEHQDLFYTAGGDAVYQILSLATASVIKKTNKPIEELFIIGVLPILQGSKMYLVLSDQTLFLVDLEASFVEPDTAVVIESKIAGNHGTVADMRFVGPDLKFLALATNSPALRVIPAPDLNAKSIVSDETTAARDHAQDSRDCFPLEVELYEGHTDLLNALDSSEDGKWLATCSKDHTVILWRYSSELEKFKAYAKFTGHAGPVTAVGLPNVMRSAWPEFIITASNDLTIKKWTVPKPGHETAEPHVVKVSDYTRRAHDKDINALSISPNDSTFATASYDKTCKVWDVDSGELQGTLANHKRGLWDVSFCQYDKLLATCSGDKTVKVWSLDTLSVVKTLEGHTNAVQRCSFINKNKQLVSTGADGLIKLWDLSTGECVRTLDAHENRIWALSVLHDGDEFVTADADGVFQFWKDCSEEQEKENLENHKRKVEQDQSLQNYMSQGDWTNAFLLAMTLDHPMRLYRVLESASAQEDGSSDFVFNEELDNVISTLNNEQIILLMKRSRDWNTNASKHAVAQKTIRCILLRHNIAALSDIPGIMNIIDAIIPYTQRHYSRLDGLVEQSYILDYSLVEMDKFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIB4</accession>
    <name>C5DIB4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ATP-dependent RNA helicase SUV3, mitochondrial</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0E11176g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553962.1">
        <property type="nucleotide sequence ID" value="XM_002553916.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIB4"/>
    <dbReference type="FunCoup" id="C5DIB4">
        <property type="interactions" value="739"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIB4"/>
    <dbReference type="GeneID" id="8292128"/>
    <dbReference type="KEGG" id="lth:KLTH0E11176g"/>
    <dbReference type="eggNOG" id="KOG0953">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010647_2_2_1"/>
    <dbReference type="InParanoid" id="C5DIB4"/>
    <dbReference type="OMA" id="FCEMIIF"/>
    <dbReference type="OrthoDB" id="6692397at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045025">
        <property type="term" value="C:mitochondrial degradosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000965">
        <property type="term" value="P:mitochondrial RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17913">
        <property type="entry name" value="DEXQc_Suv3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18805">
        <property type="entry name" value="SF2_C_suv3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000269">
        <property type="entry name" value="ATP-dependent RNA helicase SUPV3L1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001549">
        <property type="entry name" value="SUV3p ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055206">
        <property type="entry name" value="DEXQc_SUV3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050699">
        <property type="entry name" value="RNA-DNA_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022192">
        <property type="entry name" value="SUV3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044774">
        <property type="entry name" value="Suv3_DEXQc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12131">
        <property type="entry name" value="ATP-DEPENDENT RNA AND DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12131:SF1">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE SUPV3L1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22527">
        <property type="entry name" value="DEXQc_Suv3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12513">
        <property type="entry name" value="SUV3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="386"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="720"/>
            <end position="744"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00071444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR23525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="744" mass="84874" checksum="4DEAA0BAD80496B6" modified="2009-07-28" version="1">MGLLRLRQFSLKGLTAHFAASYRVYHQAAGWKKLERTEAPWGKPSFIHLPPNERKNPNYISLAFAKKVDNIYTQDQEFKRCLDSSLRWVFDNKLKAFKTPENEAKCFAWLRLREHLYVQLRDNVIDKRIASYSGSISDSFLPSSPSNLVAQLVNCDKVADNQWRAILNQETFGRYEKFNHILSKIYDFILHQEILPATIPITSSVNKTEDVDISNPAEWFPEARKWRRKIIMHIGSTNSGKTYRALQRLKQCDRGYYAGPLRLLAREVYERFKNEQIKCNLLTGEEVIEELDEMGNPAGLTSGTVEMVPLSQKFDVVVLDEIQMMGDPDRGWAWTNALLGSIAREVHLCGEKSALPLVQKITKMTGDELIVNEYERLGELRIEENALKDGLKGLRKGDCVVAFSKKRILDLKLQIEKQTDLKVAVVYGSLPPETRIQQANMFNSGEYDVLVASDAVGMGLNLSIERVIFTTHMKFNGQEMMELTSSNVKQIGGRAGRFKVSRTPANSVDGGKKASVGLVTGVDTKVLAAVKSGMEAPIEYLQSAVVWPTDEICGKLMTHFPPGTQVSELLQTLAADVEKRSAKLFTLSDLKNRLNSISLFEHMEGIPFFEKLRLSNAPVKDFPTVKKAYVQFCRTIEQRQTKSLLSYPFSFSILDPRYINSDKYSLEHYESLHNIIMLYFWLSNRYPNYFIDQQSARELKDMCEMIIFEKLDRLKRNPYMRGASTTGASHRSSRGFKPTSVRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIQ6</accession>
    <name>C5DIQ6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">L23</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">RP15L</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">RP33</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">YL14</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0E14388g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. eL19 may play a role in the last stages of translation initiation, in particular subunit joining and shedding/releasing factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the large ribosomal subunit (LSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes). eL19 lies in close proximity to the binding site for eukaryotic initiation factor eIF4G.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL19 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554104.1">
        <property type="nucleotide sequence ID" value="XM_002554058.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIQ6"/>
    <dbReference type="FunCoup" id="C5DIQ6">
        <property type="interactions" value="1082"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIQ6"/>
    <dbReference type="GeneID" id="8292274"/>
    <dbReference type="KEGG" id="lth:KLTH0E14388g"/>
    <dbReference type="eggNOG" id="KOG1696">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083919_0_0_1"/>
    <dbReference type="InParanoid" id="C5DIQ6"/>
    <dbReference type="OMA" id="NRVWIDP"/>
    <dbReference type="OrthoDB" id="5407653at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01417">
        <property type="entry name" value="Ribosomal_L19e_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1200.240:FF:000001">
        <property type="entry name" value="Ribosomal protein L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1650.10:FF:000001">
        <property type="entry name" value="Ribosomal protein L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01475">
        <property type="entry name" value="Ribosomal_eL19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035970">
        <property type="entry name" value="60S_ribosomal_eL19_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039547">
        <property type="entry name" value="Ribosomal_eL19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000196">
        <property type="entry name" value="Ribosomal_eL19_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015972">
        <property type="entry name" value="Ribosomal_eL19_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033935">
        <property type="entry name" value="Ribosomal_eL19_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057259">
        <property type="entry name" value="Ribosomal_L19e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057260">
        <property type="entry name" value="Ribosomal_L19e_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006343">
        <property type="entry name" value="PRK08570.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10722">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01280">
        <property type="entry name" value="Ribosomal_L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25476">
        <property type="entry name" value="Ribosomal_L19e_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01416">
        <property type="entry name" value="Ribosomal_L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48140">
        <property type="entry name" value="Ribosomal protein L19 (L19e)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein eL19" evidence="11">
        <location>
            <begin position="3"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="55"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="158"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="72"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00054701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00065512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00075826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00080122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00082767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00083500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR23667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="189" mass="21849" checksum="7EA65228948EFEB7" modified="2009-07-28" version="1">MANLRTQKRLAASVAGVGKRKIWMDPSETAEIAQANSRNAIRKLVKNGTIVKKPTRMHSRSRTRMMAESKRNGRHTGYGKRKGTKEARLPSQVVWIRRLRVLRRLLAKYRDAGKIDRHLYHSLYKAAKGNTFKHKRALVEHIIQAKADAQREKALKEEADARRLKNRAARERRAQRANEKREALLKEDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIQ9</accession>
    <name>C5DIQ9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0E14454p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0E14454g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554107.1">
        <property type="nucleotide sequence ID" value="XM_002554061.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIQ9"/>
    <dbReference type="GeneID" id="8292277"/>
    <dbReference type="KEGG" id="lth:KLTH0E14454g"/>
    <dbReference type="eggNOG" id="ENOG502QZJZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_325985_0_0_1"/>
    <dbReference type="InParanoid" id="C5DIQ9"/>
    <dbReference type="OMA" id="TKVACDY"/>
    <dbReference type="OrthoDB" id="5600212at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050987">
        <property type="entry name" value="AtrR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46910">
        <property type="entry name" value="TRANSCRIPTION FACTOR PDR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46910:SF37">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="9">
        <location>
            <begin position="13"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="109"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="127"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR23670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="902" mass="100477" checksum="F1AC422FF346C78D" modified="2009-07-28" version="1">MSDEALRKRVSKACDACRAKKIKCDGCDPCSNCKKVSQECGYTYVVKKRQKPPTRVSNRKILADLSQRLVRLEGILTQIRDGPLNLDDASIAESDSKFHAAPALAELRSANPLRRSSESDSAQSSELESELEPSDMDYEALPQSGSLGGYCGRHSEIDQGPSATLGSKLVNDSPVVTLPYTPVINSASDTTEVVQDSQLNTDGYLGLHSAFSIFKDSGIQWMRQKLNDNQELVRAVQQIKIIMCSDNFTNFGKFFEKTSVIDTYDFPPPETCRRLFITFLEHAAPVVPLDISSEILTLQEKFFVSPRTLNGAELFLIVAVLFVASTIQCQIACLKNDEISKWHMVQTQMVLKSLRLYQQTVLRFSADAVLYTKGVLVYCWLLENSPTPQLAYLALSTAIRLGQEVGLHLRESYFNTSGPSEALRRLVLWMNLYRYDVFLSIRTGKPALIHDGDTSSLNEWDFYQYMNAYCSGGKTALSKAAPDPDSHAWMRKLEDNLEIFASESLENLDFALTYYQWKLVQYSSKCYRYLLTCKAMVQSSFKRRLTLIKALNRDLEEWQAMLPPSLRPAGGISNLMQTLKQLETMPRRRGEAEPPWSFMDSTTASLRLKIINMHLEYYSSLLHTNCTLNRIPWCASPEPAPRDPDALAASEKCTYAARMMIKLGKLVMEPEEPNMVLRGIAFYCVFNGFLNMFWRCIEHPQSSKDDLLLLNSTCLSISAVAKVNKTEFGLKWATVAFLCLNFQKAAVETYEASCGSKDPELDLSVIRSEIESLHPILGQQAQGITVQLHVIDESRNNLRSAGEADARSERQLLNTWFSGLPGTNSGGIGVPAYGAGPGQGSTAAVLDTLEVPMVSQQAADVYGLQTGAKPEEESLLGSQVLDLGALFSAADSTTELPTFFYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJ78</accession>
    <name>C5DJ78_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0F14168p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0F14168g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554804.1">
        <property type="nucleotide sequence ID" value="XM_002554758.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJ78"/>
    <dbReference type="FunCoup" id="C5DJ78">
        <property type="interactions" value="35"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJ78"/>
    <dbReference type="GeneID" id="8293030"/>
    <dbReference type="KEGG" id="lth:KLTH0F14168g"/>
    <dbReference type="eggNOG" id="ENOG502RZPK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049349_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJ78"/>
    <dbReference type="OMA" id="YMRLILV"/>
    <dbReference type="OrthoDB" id="1932312at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR24367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="449" mass="49593" checksum="C8ACE92092A88759" modified="2009-07-28" version="1">MTQSTVDRLTQILVAQYLAKHAHEASLSQFMQETGLPRSAVEAAEYETLETLVAERVEYNESALANQLAQNSLNDAMPELDPRFGVRAWDYTQHAAAMPLERKPPSMTIDLQFGAGAGPLAGYLSASTAARTVNFYDRDLRVLRELQSTSGVVKKCGVLAGSSFYYTCGFNGSICVYNASFERVLGHQLHARIVTDIQFFQVGDSARFLCFSCGLDNHVKLHSLDLDSSSAELYDSFKLITPCTSFQLVQEPVQKCPLLLLTRLDFSHIIVLGVRGGELVEMSRIAMNSAQFSAHSFNARSMCLLNLQQTTDPTSTNKHLVPVQKGTMIAVATSHTPFMRLILLEMPDFSELSQFSDANPKIYYDKILRNMATTIAQDQFSQPIVKAFLHACGLVVGCDSGIYAVDLSNCDTWQAVPESKRVKALDVCDNRIAASYAGGDLEVWCFESR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJA4</accession>
    <name>C5DJA4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0F14828p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0F14828g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554830.1">
        <property type="nucleotide sequence ID" value="XM_002554784.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJA4"/>
    <dbReference type="FunCoup" id="C5DJA4">
        <property type="interactions" value="361"/>
    </dbReference>
    <dbReference type="GeneID" id="8293059"/>
    <dbReference type="KEGG" id="lth:KLTH0F14828g"/>
    <dbReference type="eggNOG" id="ENOG502RJFX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018313_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJA4"/>
    <dbReference type="OMA" id="TEEEIMY"/>
    <dbReference type="OrthoDB" id="4068250at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000794">
        <property type="term" value="C:condensed nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030870">
        <property type="term" value="C:Mre11 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003691">
        <property type="term" value="F:double-stranded telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051880">
        <property type="term" value="F:G-quadruplex DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043047">
        <property type="term" value="F:single-stranded telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070192">
        <property type="term" value="P:chromosome organization involved in meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000722">
        <property type="term" value="P:telomere maintenance via recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007004">
        <property type="term" value="P:telomere maintenance via telomerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18867:SF12">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18867">
        <property type="entry name" value="RAD50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="123"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="266"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="327"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="724"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="760"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="421"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="500"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="38"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="123"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="142"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="266"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="344"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="724"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="761"/>
            <end position="770"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR24393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="836" mass="91734" checksum="297B21E3EA9C8487" modified="2009-07-28" version="1">MFKKRQDPLEHLFQQSEPQTGDADKGFLHKVVSKEQEQSAYNSPFRTSQSLAAAQKRKMQKFPTVSASVSAHSSSDMGYKVIDAARRRAKDIAVPRYLQGNDERQRRRLDKLERKEDALKYKKDSQRIVDVRGGQDRAAGAAEDEGDVATDAEVEAAADAETEAEADAEAHTQTDAGADAGVAASTAESVAASTKDAEAVPATIDETPVSDTQDYVTPGAEAPDAESGIAEPETSAAIAESEVPAAIAVTETPVDGAAAEPEVPVAAAAESEVPAALPSSETAGAEPTETATVNSKEVAVGTAPDAASSVQKVPAGVDLVGEPAVAEAAGEQPATEGKINADGVPEKPKDIKETPRVPFATGIFALWTRSDKRGQPVASPNDPEFIVKFDKGYMSKALYDTLEYEEAIHKREMDDYTKEHDAKYEAKANEYEDRLTSLKSQIAELEATMETLQKDTTEKIEISEAKLSAQMIDLNAEHSSSKNVIFKETENMKAAKVEEKEGVEAKQEDVKTELEELEKLRAEIDAENREHQGRVDELTAELDAKLAAIQELNTQQAELQASIDDLQQQKDALISEANAADEQHGSNVAVLESIENKEYLPKLNAIDTKISELLTSLTVVKQETANHKTEFAAVSKRLEEEDEERKEKLRQEEENRKRLEAERLQKQREEHDQQILLLQQEHERQLAEASQKAAEHEQALLKEREQRELVERERVKLQGEKAIREQEEAKAADKALEEEIKQRNHSRALALDGAEAAVQKSQKEITEQKKTPLLPASEQKETPLLPAPVQKETPLLPAPESAQPKALTNDNSSLYEYFTEKEIRTVNSSTSAFTKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJE5</accession>
    <name>C5DJE5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">5-demethoxyubiquinone hydroxylase, mitochondrial</fullName>
            <shortName evidence="8">DMQ hydroxylase</shortName>
            <ecNumber evidence="8">1.14.99.60</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Ubiquinone biosynthesis monooxygenase COQ7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">COQ7</name>
        <name evidence="10" type="ordered locus">KLTH0F15774g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the hydroxylation of 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2) during ubiquinone biosynthesis. Has also a structural role in the COQ enzyme complex, stabilizing other COQ polypeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a 5-methoxy-2-methyl-3-(all-trans-polyprenyl)benzene-1,4-diol + AH2 + O2 = a 3-demethylubiquinol + A + H2O</text>
            <dbReference type="Rhea" id="RHEA:50908"/>
            <dbReference type="Rhea" id="RHEA-COMP:10859"/>
            <dbReference type="Rhea" id="RHEA-COMP:10914"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:84167"/>
            <dbReference type="ChEBI" id="CHEBI:84422"/>
            <dbReference type="EC" id="1.14.99.60"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="8">Binds 2 iron ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of a multi-subunit COQ enzyme complex, composed of at least COQ3, COQ4, COQ5, COQ6, COQ7 and COQ9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Mitochondrion inner membrane</location>
            <topology evidence="8">Peripheral membrane protein</topology>
            <orientation evidence="8">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the COQ7 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.99.60" evidence="8"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJE5"/>
    <dbReference type="FunCoup" id="C5DJE5">
        <property type="interactions" value="398"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJE5"/>
    <dbReference type="KEGG" id="lth:KLTH0F15774g"/>
    <dbReference type="eggNOG" id="KOG4061">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071892_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJE5"/>
    <dbReference type="OMA" id="WSTAVMG"/>
    <dbReference type="OrthoDB" id="275371at2759"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031314">
        <property type="term" value="C:extrinsic component of mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008682">
        <property type="term" value="F:3-demethoxyubiquinol 3-hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016709">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01042">
        <property type="entry name" value="DMQH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01658">
        <property type="entry name" value="COQ7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011566">
        <property type="entry name" value="Ubq_synth_Coq7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11237:SF4">
        <property type="entry name" value="5-DEMETHOXYUBIQUINONE HYDROXYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11237">
        <property type="entry name" value="COENZYME Q10 BIOSYNTHESIS PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03232">
        <property type="entry name" value="COQ7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0408">Iron</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="6 8" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="17"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="20"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR24434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="230" mass="26047" checksum="AB621E92ABFC8A8D" modified="2009-07-28" version="1">MLRKSVSRPASRGFSVLKSLKTTESPKSTQNAKPNDRFKPLSDAQVAYLDRVVRVDQAGELGANYIYAGQYFVLASKYPHLKPVLHHMWEQEIHHHNTFNEMQLKRRVRPSLITPLWKLGAFIMGSGTALISPEAAMACTEAVETVIGGHYNDQLRCLNNQYELERNDGTKGHSQELSSLIATIKQFRDDELEHLDTAIEYDSHKAVPYILLTEGIKNVCRVAIWTAERV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJU1</accession>
    <name>C5DJU1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0F19118p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0F19118g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the replication factor A protein 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555017.1">
        <property type="nucleotide sequence ID" value="XM_002554971.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJU1"/>
    <dbReference type="FunCoup" id="C5DJU1">
        <property type="interactions" value="961"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJU1"/>
    <dbReference type="GeneID" id="8293253"/>
    <dbReference type="KEGG" id="lth:KLTH0F19118g"/>
    <dbReference type="eggNOG" id="KOG3108">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051033_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJU1"/>
    <dbReference type="OMA" id="GRIECNK"/>
    <dbReference type="OrthoDB" id="25571at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005662">
        <property type="term" value="C:DNA replication factor A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04478">
        <property type="entry name" value="RPA2_DBD_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040260">
        <property type="entry name" value="RFA2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014646">
        <property type="entry name" value="Rfa2/RPA32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014892">
        <property type="entry name" value="RPA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13989">
        <property type="entry name" value="REPLICATION PROTEIN A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13989:SF16">
        <property type="entry name" value="REPLICATION PROTEIN A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08784">
        <property type="entry name" value="RPA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036949">
        <property type="entry name" value="RPA32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OB" evidence="7">
        <location>
            <begin position="70"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Replication protein A C-terminal" evidence="8">
        <location>
            <begin position="191"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="11"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="20"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR24580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="278" mass="30420" checksum="85763FF0ED721E13" modified="2009-07-28" version="1">MATYQPYNEFSTVSGGGFDNSQSSGRPNSGNGGSGSSSTLTPVTIKQVQESKQIVQDGPFVVNNLELHHVSFVGVVRNVVDNTSNINLTIEDGTGQIEVRKWSDDSNDMANAAQDEQPDAAESSQVAQMYEVGTYVRVFGALREFSGKKNVQFAVIKPIDNFNEVITHYLEAMKWHAIANGKLESPVNLQPNSEQTGEEQNLFVQDNSASEPKTALHKILEFCTAQCEGKDASSFAVHTKLIAQSLALSEPDVKMYCQTLVDQGFIYPTFDDSSFFVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJU5</accession>
    <name>C5DJU5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">prephenate dehydratase</fullName>
            <ecNumber evidence="2">4.2.1.51</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0F19206g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-phenylalanine biosynthesis; phenylpyruvate from prephenate: step 1/1.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.51" evidence="2"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555021.1">
        <property type="nucleotide sequence ID" value="XM_002554975.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJU5"/>
    <dbReference type="FunCoup" id="C5DJU5">
        <property type="interactions" value="135"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJU5"/>
    <dbReference type="GeneID" id="8293257"/>
    <dbReference type="KEGG" id="lth:KLTH0F19206g"/>
    <dbReference type="eggNOG" id="KOG2797">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035008_5_1_1"/>
    <dbReference type="InParanoid" id="C5DJU5"/>
    <dbReference type="OMA" id="PLMIYRE"/>
    <dbReference type="OrthoDB" id="983542at2759"/>
    <dbReference type="UniPathway" id="UPA00121">
        <property type="reaction ID" value="UER00345"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004664">
        <property type="term" value="F:prephenate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009094">
        <property type="term" value="P:L-phenylalanine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04905">
        <property type="entry name" value="ACT_CM-PDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13532">
        <property type="entry name" value="PBP2_PDT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000254">
        <property type="entry name" value="Prephenate dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008242">
        <property type="entry name" value="Chor_mutase/pphenate_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001086">
        <property type="entry name" value="Preph_deHydtase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21022">
        <property type="entry name" value="PREPHENATE DEHYDRATASE P PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21022:SF19">
        <property type="entry name" value="PREPHENATE DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00800">
        <property type="entry name" value="PDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001500">
        <property type="entry name" value="Chor_mut_pdt_Ppr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51171">
        <property type="entry name" value="PREPHENATE_DEHYDR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0584">Phenylalanine biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prephenate dehydratase" evidence="7">
        <location>
            <begin position="3"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR24584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="332" mass="36721" checksum="AF4AE05590418717" modified="2009-07-28" version="1">MVNIAFLGPLGTYTHQAALQQFPDKDANYIPLAGIPQCFEALIKDASLDFAVVPLENSTNGQVVFTYDLLRDLMNNCKEYKGNEAIPELEVVAEQYVSIELCLIAAQPMSLDDLNSETRVTIHSHPQVWGQASSYLKNLGQRVQELKTVDSSSTSGAVKICCEAGQPSGNEVVLAIASRTAATVNGAAIVDSPINDRKGNTTRFLTLRRRSTDFPPIPARPPVAKGVEQVALLTFIGKQDTSPGSLVDVLNVLKDHEINMCSISSRPYHHPDSSSKADESAHRKWQYIFFIEFNHADGVSWDQVVHEMGSKTLKFCFWGTFYRNGRYYETST</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJX9</accession>
    <name>C5DJX9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0F00110p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0F00110g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554217.1">
        <property type="nucleotide sequence ID" value="XM_002554171.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJX9"/>
    <dbReference type="STRING" id="559295.C5DJX9"/>
    <dbReference type="GeneID" id="8292403"/>
    <dbReference type="KEGG" id="lth:KLTH0F00110g"/>
    <dbReference type="eggNOG" id="ENOG502RZTT">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065416_1_0_1"/>
    <dbReference type="InParanoid" id="C5DJX9"/>
    <dbReference type="OMA" id="HDNACGP"/>
    <dbReference type="OrthoDB" id="2013972at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591:SF109">
        <property type="entry name" value="METHYLTRANSFERASE TYPE 11 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase" evidence="1">
        <location>
            <begin position="45"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="278" mass="31012" checksum="DC6FC64E216AFFA6" modified="2009-07-28" version="1">MMSDAWGLQAKEYAEKLQKGPPKASVDAILQAVDSLLPFEKAKSILDNGCGTGLGTQTLIDKYGHQLPDLIHLVAADLSPGMIESVRMTKAAHKGNRFWDRLELQVCSVDNMSKTQNSCFSHIIASLVFFFAKPEATFSEAYRILQPGGVIGFTSFRENQWMDLRNVAKKINPGLEPAPLPAEWASEEWIKRQLEKANFIDIKIEPMEVYVELDDSLPLATLLVRSGIPEMSAVFQSLTDEEKDRAVQLIVEELAERYPKRPAKIPGILLVSSARKQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>C5DK01</accession>
    <name>C5DK01_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">KLTH0F00704p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0F00704g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
            <location evidence="1 8">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family. SPB1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554239.1">
        <property type="nucleotide sequence ID" value="XM_002554193.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DK01"/>
    <dbReference type="FunCoup" id="C5DK01">
        <property type="interactions" value="1233"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DK01"/>
    <dbReference type="GeneID" id="8292430"/>
    <dbReference type="KEGG" id="lth:KLTH0F00704g"/>
    <dbReference type="eggNOG" id="KOG1098">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009422_8_1_1"/>
    <dbReference type="InParanoid" id="C5DK01"/>
    <dbReference type="OMA" id="QRKDKYY"/>
    <dbReference type="OrthoDB" id="1287559at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016435">
        <property type="term" value="F:rRNA (guanine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008650">
        <property type="term" value="F:rRNA (uridine-2'-O-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000004">
        <property type="entry name" value="AdoMet-dependent rRNA methyltransferase SPB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01547">
        <property type="entry name" value="RNA_methyltr_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03163">
        <property type="entry name" value="RNA_methyltr_E_SPB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050082">
        <property type="entry name" value="RNA_methyltr_RlmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002877">
        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015507">
        <property type="entry name" value="rRNA-MeTfrase_E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012920">
        <property type="entry name" value="rRNA_MeTfrase_SPB1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024576">
        <property type="entry name" value="rRNA_MeTfrase_Spb1_DUF3381"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028589">
        <property type="entry name" value="SPB1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920:SF13">
        <property type="entry name" value="PRE-RRNA 2'-O-RIBOSE RNA METHYLTRANSFERASE FTSJ3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920">
        <property type="entry name" value="RIBOSOMAL RNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11861">
        <property type="entry name" value="DUF3381"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01728">
        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07780">
        <property type="entry name" value="Spb1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3 8" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6 8" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribosomal RNA methyltransferase FtsJ" evidence="10">
        <location>
            <begin position="25"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3381" evidence="12">
        <location>
            <begin position="236"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosomal RNA methyltransferase SPB1-like C-terminal" evidence="11">
        <location>
            <begin position="605"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="358"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="471"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="566"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="471"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="513"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="594"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="617"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="634"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="159"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF11861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR23802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="830" mass="94297" checksum="2ABA64F8CBF3BC0E" modified="2009-07-28" version="1">MGKTQKKNSKGRLDRYYYLAKEKGYRARSSFKIIQINEKFGHFMEKSKVVIDLCAAPGSWCQVASNLCPVNSLIIGVDIVPMKPMPNVITFQSDITTEDCRSKLRGYMKTWKADTVLHDGAPNVGLGWAQDAFTQSHLTLQALKLAVENLVVGGTFVTKIFRSKDYNKLIWVFSQLFDKVEATKPPASRNVSAEIFVVCKGFKAPKKLDSRLLDAREVFEELPDGPQNNEAKIFNPEKKVRKRQGYEEGDYLLYHHKPIMEFVKCEDPIDMLGNLNKFTVNSEDHEWKILKKLKQTTPEFMMCIEDLKVLGKKDFKMILKWRKAARELLGIEKEEEPVVETTHLTEEEQIQSEVQALQEKQRLSQKREKRKKNEMKQKELTRMQMNMLTPMDIGVESAELGRESLFNLKSAEKTGILDKLAKGKRRMVFTQGELAQDNDVHIADDAPLADRDDAAEADELESQLNSMYQNYKEKKAEKDAKYRAKQARGGDDNEWTGFGDEDSEHSSAVEDDHVSEDENSDLSDSDDDEAINRLIAKMKNKEGENGLSSKAQALFNQSIFEGVEADLPAPEEPVNASVTAHVERLESLGKNQGDENNLSQESSSDSENSDFEVVAREDEDQVMDSDFDSDDENQSQKEKHAEEIDIATVEAMTLAHQLALGQRTKHDLVDEGYNRYAFRDTENLPEWFLEDERPHSKINRPITKEAAIAIKEKMKALNARPIKKVAEAKARKAMRAVARLEKIKKKAGLINDDGDKSEKDKADEIAKLMRKVTKKQKQKPKVTLVVATGKNRGLSGRPKGVKGKYKMVDGVLKNEQRALKRIAKKHHKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DK04</accession>
    <name>C5DK04_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cytochrome b-c1 complex subunit 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0F00770g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the UQCRB/QCR7 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554242.1">
        <property type="nucleotide sequence ID" value="XM_002554196.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DK04"/>
    <dbReference type="FunCoup" id="C5DK04">
        <property type="interactions" value="235"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DK04"/>
    <dbReference type="GeneID" id="8292433"/>
    <dbReference type="KEGG" id="lth:KLTH0F00770g"/>
    <dbReference type="eggNOG" id="KOG3440">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115154_1_0_1"/>
    <dbReference type="InParanoid" id="C5DK04"/>
    <dbReference type="OMA" id="MAKWEAN"/>
    <dbReference type="OrthoDB" id="425749at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045275">
        <property type="term" value="C:respiratory chain complex III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006122">
        <property type="term" value="P:mitochondrial electron transport, ubiquinol to cytochrome c"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1090.10:FF:000001">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1090.10">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003197">
        <property type="entry name" value="QCR7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036544">
        <property type="entry name" value="QCR7_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12022:SF0">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12022">
        <property type="entry name" value="UBIQUINOL-CYTOCHROME C REDUCTASE COMPLEX 14 KD PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02271">
        <property type="entry name" value="UCR_14kD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000022">
        <property type="entry name" value="Bc1_14K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81524">
        <property type="entry name" value="14 kDa protein of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR000022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR23805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="128" mass="14712" checksum="3237A80260CE99EF" modified="2009-07-28" version="1">MPQTITSIVKMGDFILRSPALSKVVVPVAQQFVKFAGYRQLGLKFDDIIAEENDIAQTALRRLPEDEGYARAFRMIRAHQSELTHHLLPKSQWVKPEEDTLYLTPYLLEAEAEAKEREELDNLQLTTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>C5DK10</accession>
    <name>C5DK10_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0F00902p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0F00902g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HyuE racemase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554248.1">
        <property type="nucleotide sequence ID" value="XM_002554202.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DK10"/>
    <dbReference type="FunCoup" id="C5DK10">
        <property type="interactions" value="56"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DK10"/>
    <dbReference type="GeneID" id="8292439"/>
    <dbReference type="KEGG" id="lth:KLTH0F00902g"/>
    <dbReference type="eggNOG" id="ENOG502RZ0H">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053002_1_0_1"/>
    <dbReference type="InParanoid" id="C5DK10"/>
    <dbReference type="OMA" id="ITSNKEW"/>
    <dbReference type="OrthoDB" id="412018at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047661">
        <property type="term" value="F:amino-acid racemase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015942">
        <property type="entry name" value="Asp/Glu/hydantoin_racemase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052186">
        <property type="entry name" value="Hydantoin_racemase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053714">
        <property type="entry name" value="Iso_Racemase_Enz_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28047">
        <property type="entry name" value="PROTEIN DCG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28047:SF5">
        <property type="entry name" value="PROTEIN DCG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01177">
        <property type="entry name" value="Asp_Glu_race"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="281" mass="30070" checksum="E7168B21B58C21E8" modified="2009-07-28" version="1">MRIVLSSADRTPPATMRILLVNPNSTEAMTQAAGARVQAYLASAYGAAAASLNVSLFTAPPDAPRMIDGEDASRESAAKCLPLLCRRAEPDALQYFDTFDGVLIACFSDHPLVHMLQQRAERATQVTCIFHASLTACLARAGARFSIITSNDEWVALLDAAAARLLGAEPPFASSKAPFKGTVASSVPVLDLHDPANLHAIAKRIYEENVVRLDTSTVILGCAGFSGMEAALSQEITKIAAQRDPGRTLHVTLVDSVISGVETLALMCRLRQDYDSNMQQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>C5DK52</accession>
    <name>C5DK52_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0F01826p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0F01826g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554290.1">
        <property type="nucleotide sequence ID" value="XM_002554244.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DK52"/>
    <dbReference type="FunCoup" id="C5DK52">
        <property type="interactions" value="670"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DK52"/>
    <dbReference type="GeneID" id="8292481"/>
    <dbReference type="KEGG" id="lth:KLTH0F01826g"/>
    <dbReference type="eggNOG" id="ENOG502QPWC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017827_0_0_1"/>
    <dbReference type="InParanoid" id="C5DK52"/>
    <dbReference type="OMA" id="DILMGWI"/>
    <dbReference type="OrthoDB" id="6718656at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030907">
        <property type="term" value="C:MBF transcription complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033309">
        <property type="term" value="C:SBF transcription complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.260.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
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        <property type="entry name" value="SWI6-like"/>
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        <property type="entry name" value="ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43828:SF3">
        <property type="entry name" value="CHROMO DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18530">
        <property type="entry name" value="Swi6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Swi6 N-terminal" evidence="6">
        <location>
            <begin position="7"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="356"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="97"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="438"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="97"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="107"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF18530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR23853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="663" mass="74464" checksum="3D3E65D04A442CCB" modified="2009-07-28" version="1">MDIREYEHHYDLNGQATVVKLVRDLNKDSFLVDALVPLLASAANVEKSNGDWRNLVLQKFGILVERDGQGNEWISRGKTWELLKILNLHDAYRDVFEEPGDTDEDDNSARFDGETREKRSLKGTTHQLGSPLKKLKPAEAFQTVRTFSHDLDLASPLAVQLAKKPLKTFQPEERVKLEHLLQHILFPDDQSIQFETALRNLEETYPQTRLNLDIPIDEHGNTSLHWLCSIANVRLTKDLIHTGADRLLGDMSGESALVKAVKSVNNYDSGTFEELLDYLYPCLILLDEMGRTVLHHIVITSGMPGCASAAKYYLDILMGWIVKKQAREVAGDADIIMTTVDLKWFISHVLNARDINGDTCLNIASRLGNVSIVEALLDYGADPRISNNSGLRPVDFGAGASKIEASTANADNGATVTALGPDSAVLINNIQSLINTISQDYDMEIKQHKEQLKKLHSELKTQRELLSSSRDRLAQARQLRDEHTQLTEQVSNIQQAIGEEDASFKKVSDELGINDESLDMAEFDADEPFRIEFIYEFLEKKLEAEYSGDLEKFLGNASLDELTAQLMEESSGQEESIPPAVLLRARICAYEKNEESLNKSLESVHQKQADLEGRFRRVLSLCLKIDEDKVDDMLDGLLQAISTEDPEDIDTDEMQSFLSKHNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5DK56</accession>
    <name>C5DK56_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0F01936p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0F01936g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="protein sequence ID" value="CAR23857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="component" value="Chromosome F"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="P:DNA damage checkpoint signaling"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="DNA_damage_chkpnt_Lcd1"/>
    </dbReference>
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        <property type="entry name" value="LCD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
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        <location>
            <begin position="124"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="80"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="26"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="142"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR23857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="713" mass="81545" checksum="BBDDFF4C8BAFC4FB" modified="2009-07-28" version="1">MDDWDDDDEDDLIEALSKKPPREPSFGSTQSFQRTQNDPKGTETQSAVAVSAEEAKDIEMKLLKAQGEASMLRDKLALFEAEKERERKTQTQKQKELELQHQHELDLIKADLQKAEDEKKFLAFSGRSSHRSPGRHAAANTPEASSGESVSAATKKRKLEEPPRRYITLKPNRIVSDEVDLFVNSAISHRLAGSRLSTLEILNNIYLGHVKDFKFKILDIAGDGPVGKGIFDMLMSHIRSPLKLDQFIEILLECLAMLIKEISQHEKECDTAVPFLVALMHHCITFRPSAIHTMALKDLFNFMVDLIRTHRSVLKRPLRKSDLELDVGPDIFQYEFIRILTVLYAFDVLEDGLKIVQSLPVSFQAAFMDDAFRGAVDDISRYSLTISYQPVINVIHNTVEMFNCIANMLLEAPELKEKVPVRWWDNIINRLYHILNKGTSNTARNELELSTLHLFDEINIFGLIRNIGDNHNGQLISLLVKENEPWHLPRVIMRDFPKEQGITNRATDVEWWAVNMKIGIVKLFEKLAIAYRDGPVERNMLKVLARLLAQTQEVLLTVLLGEDSENIHVYYEFFSQTLKLIYHMWKSGQWELRLLREAEAELTVCLWRVVFGTTHEGSRVEMAEHKTLADRFDELRLQQDAELYEDAFENEVPDFVAEETRAQSFSRLKEIMGIGAEETTRQMAKTMLEGITSMEDADALYMAMQAGSAELGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DK82</accession>
    <name>C5DK82_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0F02508p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0F02508g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
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            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554320.1">
        <property type="nucleotide sequence ID" value="XM_002554274.1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="STRING" id="559295.C5DK82"/>
    <dbReference type="GeneID" id="8292512"/>
    <dbReference type="KEGG" id="lth:KLTH0F02508g"/>
    <dbReference type="eggNOG" id="KOG2076">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002391_0_1_1"/>
    <dbReference type="InParanoid" id="C5DK82"/>
    <dbReference type="OMA" id="SSPNMKF"/>
    <dbReference type="OrthoDB" id="9991317at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000127">
        <property type="term" value="C:transcription factor TFIIIC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006383">
        <property type="term" value="P:transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039340">
        <property type="entry name" value="Tfc4/TFIIIC-102/Sfc4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23082:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR 3C POLYPEPTIDE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23082">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIIC TFIIIC , POLYPEPTIDE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="524"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="332"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="596"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="41"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="58"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="332"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="343"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="638"/>
            <end position="651"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR23883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1055" mass="123042" checksum="503A17898F262912" modified="2009-07-28" version="1">MVEENANENGEEKNIDEEEFYGNLDELRDLIDDENDGRTDGNVTGFSDSTASEDEVFDERLLEQDGTHLDAEQLEEKGSIDENHYEDADLLAEFSDYGEFSDDEYDEQDFMDAIREANNFKVKRKNKAKKKKESVGKAPKRNVRERVLDPEVAQLLSEANEAFVRNDIQVAEHLYNEVIKKDVKNFAAYKTLGDIYQLQGRFNDCCNSWFLAAHLNPSDWEFWKVVATLSSDLGHIRQAIYCYTRALRMNSDDWECLYGRSILYKKTNQLGRALEGFQRLHNNNPYDGNLLRELAILYVDYNRINEAIELYIKIFEANVQKREALISAAENAVESSGESGNESNDDESEDLDEAEEGYKEYSHINWKKIHKKFRCIAFDWSSLNILAELFLKQSGENTGIKTIKKCARWIQKREKQTFWDNVNDDSEFDKRRFKNSKFDALSKEEKEKSYILPIDIRVRLGLLRLNNKHVLEAMNHFQALYDETFVDITDLFFEVACALTKAERFQEAIEFFAPLLQIPDYCTLEVYKPLSKCYKEVEDYENAKLIFKKLVELAPEDLETKLTLAEIYYHLEDHETFNALLLEVVETRKQQATDIRQDVAGLNDQPNQGPEERSTNELGKPLLEDIHIRKTAAKRKRTPQDAERERKEREKRITSNVLDKYNRLKIYKEGMEKGQESQTTLWIDTASDLIDVFSSVKNFFVKSRSKKFVGVIKRSRKFNKIIDYKIKRLSKLSEGENLLDGLPLLEERVSLTSSTELRGLTYQQWFELFMELSLVITKLQSVEDGLSVIETAQEVNVFAQDPEKVKMMKFVNLAIVLRLEDEDELVENLRGVLNQFQFNRKVFQCFMFCVARGQMSSDILSSTVQQKFFLRQLKAYDSIRYGQHVAGQASVTNKKVSNPDKFKSPYLHYIYAMLLYSSKGFLLALQYLNRLEPELYEDPMINLMTGLAHLHRSMQRLTASRHFQILHGLRFLYRYHDIRANRYSSTEKQEADYNIGRAFHLLGLFSIATRYYQKVMTEYGDEKLKRHAAYNCILIYNESGNPELANSLMEKYLSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKA4</accession>
    <name>C5DKA4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0F03014p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0F03014g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554342.1">
        <property type="nucleotide sequence ID" value="XM_002554296.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKA4"/>
    <dbReference type="FunCoup" id="C5DKA4">
        <property type="interactions" value="28"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKA4"/>
    <dbReference type="GeneID" id="8292534"/>
    <dbReference type="KEGG" id="lth:KLTH0F03014g"/>
    <dbReference type="eggNOG" id="ENOG502QRWI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_449939_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKA4"/>
    <dbReference type="OMA" id="LMANFMT"/>
    <dbReference type="OrthoDB" id="5600212at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000435">
        <property type="term" value="P:positive regulation of transcription from RNA polymerase II promoter by galactose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051127">
        <property type="entry name" value="Fungal_SecMet_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47424">
        <property type="entry name" value="REGULATORY PROTEIN GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47424:SF3">
        <property type="entry name" value="REGULATORY PROTEIN GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="7">
        <location>
            <begin position="15"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR23905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="612" mass="69827" checksum="43E1C32251768F85" modified="2009-07-28" version="1">MTAARSGKRKVGRRACMLCKLKKLKCDGNYPCGRCAKSGVPSECGYEADRRKCRAYGSDGTKAYVFKSSPFSSQHPSEGSFKNEATVHHLSEHKIETSPDPLIKSIQDATLSFNERKDSEYDMWNFKPMVFNFNNFNLQDVGFDDFLRVFDDVDPFSTEDGSDQFPFAEPAELPLGAPFGNDNKESRLERSDLIEILFQSDYHAPPGISKDHLLDLERRHEDLQDDSADSNDIFLLSTTLCLGALTSHKRELLRTRSEGFVSRSSIPDRAARAFNYYTIAKNLIPDVLLHSSIDGFCGLVLMANFMTMMVSLEEQFYLSANALQIAVTLRLHEREKFNELVKINSHNIGLFFLFWNIWCSSCMLGSFLGVKPTLPLADIGIPLPFDMAVTGKISSLSLKFMQLRIQIASLLTKISRYYIEDHMTTATFMGLEEELHDISNQVAKLKCLPILDEHTYHRSRLLILELASMESQVALLLYRSNLLCKRSFEAVHTAKKIVYEIWSHYSKQFEKNERDLINHLDWNFSYPLRTAFLTLWIACIVLRKYQRLLSFLQDDNLTEYTLAIEVLQDLVKLLPVHQNLLNLITSLGEAGKPHSSGPDRSSHFWMSLLTEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKB9</accession>
    <name>C5DKB9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0F03344p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0F03344g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554357.1">
        <property type="nucleotide sequence ID" value="XM_002554311.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKB9"/>
    <dbReference type="FunCoup" id="C5DKB9">
        <property type="interactions" value="56"/>
    </dbReference>
    <dbReference type="GeneID" id="8292549"/>
    <dbReference type="KEGG" id="lth:KLTH0F03344g"/>
    <dbReference type="eggNOG" id="ENOG502R3QS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_429070_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKB9"/>
    <dbReference type="OMA" id="MHLKFND"/>
    <dbReference type="OrthoDB" id="4064185at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR23920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="651" mass="75174" checksum="00A4A26173409121" modified="2009-07-28" version="1">MIRPLKGLTTQRAAPAYLRVCMLHTERETQLKITTSELNYCILNQKTYSTSQLDRKIKEYWKVSKANSTTNFETPSVQPRIFLTGTDLFKYDNKQNKVSAVYRCFVRWEIIRAQPPLRGKLFANNLIHSSPLSPSDLKNTDIDILPFLIHNCITYEDIPNAIDLFIWYNTKTPGKELHRTLINAMIQCIAESSQIEPHYHLLKFTQLMNFLNEKSIEHTLSEHQALQISNKAMSLDNNPLLTKKVLSYVLDVAYNKSNTVRTAQVEALYSLISSDYKSNNAAGVLLHWTAVRDHYTTISKHDTRILYKIIKLFTKQKAYRQRCAELISSIEPQLYVNNALLLPSIINFASATKSYSLATKIMSNITTYSNFNTQKQILKSRFFLSTLLRLHLTFEDSIGVERILTEIRKEHNSLTSADYQVIVSHLLKSRKPQDLLKALTLSNKIEGLKGIPSITTIINHLTLLKTHEVEKLNLNVGATIHNLLLKAHNNDPAHKTKLWDILASIFVRSLTTYKHNTTPWWTNEIYDKTTKVDFLKLCYETSKNKTPMDITVNPFVSPNPKDTVIKLSATNIIVILRTIAREANHHRRSDVFLWCMNEMTKNGMSEEEIELDWNNMEKHQIRRFKNKTKNGITKNLQKHGLKSFKTLLRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKH9</accession>
    <name>C5DKH9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">KLTH0F04796p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0F04796g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554417.1">
        <property type="nucleotide sequence ID" value="XM_002554371.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKH9"/>
    <dbReference type="GeneID" id="8292612"/>
    <dbReference type="KEGG" id="lth:KLTH0F04796g"/>
    <dbReference type="eggNOG" id="ENOG502QU6S">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024407_1_0_1"/>
    <dbReference type="InParanoid" id="C5DKH9"/>
    <dbReference type="OMA" id="TWAITLC"/>
    <dbReference type="OrthoDB" id="1288932at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43562">
        <property type="entry name" value="NAPA-TYPE SODIUM/HYDROGEN ANTIPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43562:SF3">
        <property type="entry name" value="SODIUM ION_PROTON EXCHANGER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0050">Antiport</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="9" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="37"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="67"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="96"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="126"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="159"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="192"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="236"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="426"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="512"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="12">
        <location>
            <begin position="41"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="12">
        <location>
            <begin position="402"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="322"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="346"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="348"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="363"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR23980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="558" mass="60207" checksum="C22F12869E1AC750" modified="2009-07-28" version="1">MSQAAALSYHEPNIIVILILASFLIILNIVGRALDSLLYCGLLGQIFIGVAWGLPGGKWLSLPFQQSVVQLGYLGLILLVYEGGLSTDFRAMKANFMLSTFLALTGVIAPIGMSFVLMSLSHATPLQSFAAGASLCSTSLGTTLTVLKISGLKQSRLGVVLTCAAMLDDVVGLVMAQIISNLGGSGSINPVIIIRPILVSFAFAVVVPLVCVFLIKPLACRMQHCHLKKVRFFPTTVQMTFLAHTLILFGFVVGSSYAGTSNLYAAYLAGACIGWYDNEVNRGAFVERTSATLANSNKGYFSPKKIGQMLALAPQAEAFSKSSKHPHSDFETPKLPPSAAIRCSGTDDLNDNVEEENTEDKQLNPNDTVTGSETSISTSNLRSGHVSGLHIWEAYYEVPLSTVLKPFFFASIGFSIPISEMFDGNIIWKGIVYAILMTLGKFICGAWLLRSICFKRFANDVESGGHIWGFPKARSLIPPSIMGYAMVARGEIGFLISALAESRGIFTPEQYYIVTWGIVLCTIVGPLMVGLLTSKVRKIRDSKELAASAEHDPMGIWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKK3</accession>
    <name>C5DKK3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 16">Phosphatidylinositol transfer protein SFH5</fullName>
            <shortName evidence="16">PITP SFH5</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">KLTH0F05434g</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Non-classical phosphatidylinositol (PtdIns) transfer protein (PITP), which exhibits PtdIns-binding/transfer activity in the absence of detectable PtdCho-binding/transfer activity. Regulates PtdIns(4,5)P2 homeostasis at the plasma membrane. Heme-binding protein that may play a role in organic oxidant-induced stress responses.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)(in) = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)(out)</text>
            <dbReference type="Rhea" id="RHEA:38691"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:38692"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 16">Endoplasmic reticulum membrane</location>
            <topology evidence="2 16">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Microsome membrane</location>
            <topology evidence="16">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the SFH5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554441.1">
        <property type="nucleotide sequence ID" value="XM_002554395.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKK3"/>
    <dbReference type="FunCoup" id="C5DKK3">
        <property type="interactions" value="70"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKK3"/>
    <dbReference type="GeneID" id="8292639"/>
    <dbReference type="KEGG" id="lth:KLTH0F05434g"/>
    <dbReference type="eggNOG" id="KOG1471">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045138_0_1_1"/>
    <dbReference type="InParanoid" id="C5DKK3"/>
    <dbReference type="OMA" id="KRVVTWN"/>
    <dbReference type="OrthoDB" id="75724at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008526">
        <property type="term" value="F:phosphatidylinositol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017157">
        <property type="term" value="P:regulation of exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042938">
        <property type="entry name" value="Sfh5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47669">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL TRANSFER PROTEIN SFH5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47669:SF1">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL TRANSFER PROTEIN SFH5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 16" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 16" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0349">Heme</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="12 16" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 16" id="KW-0492">Microsome</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 16" id="KW-0813">Transport</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="17">
        <location>
            <begin position="92"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00024146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00024180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU367059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAR24004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="285" mass="33272" checksum="B1AC77BB91CF78C3" modified="2009-07-28" version="1">MKFASDLEKKTFEKLVEQLPSLIEKKCQGYDEMYGYKLLPGEHYDESIAHALVYKYCKAYKFQYDEVASNLCSTLNWRREFDPLSAAFSERHDETLNNVGLLTKYDDEQSNRKVVTWNLYGELSKQKQVFADVNKFLRYRVGLMERSIGLLDFKDETNDYVAQVHDYDGVSMWRMDPDIKKCTKQVIAVFQKHYPEMLSAKFFINVPSLLTWVYDVVKRFVNEETRRKFVVLNDGTKLGQYLPAAPSKLYGGNSKQTLEEQNVLEVKPTPYALYLFEQKVGADVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKP0</accession>
    <name>C5DKP0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0F06270p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0F06270g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554478.1">
        <property type="nucleotide sequence ID" value="XM_002554432.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKP0"/>
    <dbReference type="FunCoup" id="C5DKP0">
        <property type="interactions" value="24"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKP0"/>
    <dbReference type="GeneID" id="8292677"/>
    <dbReference type="KEGG" id="lth:KLTH0F06270g"/>
    <dbReference type="eggNOG" id="ENOG502QVEK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050744_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKP0"/>
    <dbReference type="OMA" id="MGFPKNL"/>
    <dbReference type="OrthoDB" id="270293at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005537">
        <property type="term" value="F:D-mannose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07308">
        <property type="entry name" value="lectin_leg-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051136">
        <property type="entry name" value="Intracellular_Lectin-GPT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12223:SF45">
        <property type="entry name" value="RE50040P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12223">
        <property type="entry name" value="VESICULAR MANNOSE-BINDING LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950518" evidence="2">
        <location>
            <begin position="30"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="373"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR24041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="414" mass="46931" checksum="8F42FC6BFD32C9A9" modified="2009-07-28" version="1">MRGWRAWTQAALIALVFLGFRLVFKPSQNAEMRSGPFPLWRTRVQREPNADASLSIPLLDKINNFWLVDGATQIRNFGNIRLTSRGQLGQYGVVVSNGAGDNVLDDFETVVSFKIHNTGVSSQFMGDGMVVAISPEKDYIQQNMMSSYGRSQYEHNSGGIIGNDRGLMGLPRNLPGLAVVIDTYKNDPKTRLKPPFMSVLLNMDPRKHHYSAATDGTESTGHNLCPLIKLKRSLMSGKDTKLRIIYLESIGFLKVDIKYPDSNEWIELYQQDSNLYLPKNRVTGQRYIAIGALTGQATQTVEIQGVETSEFHWDEHEEEGFDYAKEMQYFLAQEYGERVKIGEDDYGKWLQVKRQGGTDQDPVARNKSKHSTLYYMAKISFVMFFLYGLSLSIRISIKRAVRLRRKKSQASILG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKS5</accession>
    <name>C5DKS5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0F07128p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0F07128g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFB1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554513.1">
        <property type="nucleotide sequence ID" value="XM_002554467.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKS5"/>
    <dbReference type="FunCoup" id="C5DKS5">
        <property type="interactions" value="1015"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKS5"/>
    <dbReference type="GeneID" id="8292716"/>
    <dbReference type="KEGG" id="lth:KLTH0F07128g"/>
    <dbReference type="eggNOG" id="KOG2074">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019188_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKS5"/>
    <dbReference type="OMA" id="NRPNFDM"/>
    <dbReference type="OrthoDB" id="360521at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000439">
        <property type="term" value="C:transcription factor TFIIH core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13229">
        <property type="entry name" value="PH_TFIIH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3970.10">
        <property type="entry name" value="BSD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005607">
        <property type="entry name" value="BSD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035925">
        <property type="entry name" value="BSD_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027079">
        <property type="entry name" value="Tfb1/GTF2H1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013876">
        <property type="entry name" value="TFIIH_BTF_p62_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12856">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03909">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08567">
        <property type="entry name" value="PH_TFIIH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00751">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140383">
        <property type="entry name" value="BSD domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50858">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BSD" evidence="7">
        <location>
            <begin position="143"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="BSD" evidence="7">
        <location>
            <begin position="221"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR24076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="621" mass="69334" checksum="33E9023D253AC444" modified="2009-07-28" version="1">MSHSGAAVFKKVSGILTIDEEASPAELLWRSTDGDKTHKVALNTIDKLQATPASSEKMMLRLIGKANEAKKRKDADGNEVKAPLPSHVFSFNNRTVMDNIKETLQLIIARYKDEEVFEEKKRKESSLAPAPSEAAPLINTSTLDDSLSREKLLTNLKLQQSLLKENKGLMKTFQEAVINSGLPPDEFWSTRIPLLRAFALTTSQRTGPYNVLSTIKPVASSDNKVNVSVSREKIMTIFQTYPIVKKAYDDNVPKNFKEQEFWARFFSSKLFRKLRGERIMQSDRGDMIIDRYLTLDQEFDRKDDEMLQHPVKKLIDLDGNQYDDPERKGNKPDFTMIPGVDVNGNTDGVVDILKGMNRLSQKMIKSLENEYSRTNLAADDADKEEREELLYSDLEEEDEATYIEIKVKQRMAAVRETAGTEHKGERFKVGTKEISHQLNLLLDGLTKSIDLTKVANDDKKANNTANQNVIKAVKINAKQAKNTNFNAVVGANTGITTIESTKEIETPLPGDLLESCRVLHGTCCEFLKHFYIHFQSGEARQAPTVKKLYGFLKQCPEKVNKLLNKVQGSTDKQGETLASSCNAYLSSVLGSLNLAIEKYELAVAEANAANAANAASSAEVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKU3</accession>
    <name>C5DKU3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0F07568p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0F07568g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554531.1">
        <property type="nucleotide sequence ID" value="XM_002554485.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKU3"/>
    <dbReference type="FunCoup" id="C5DKU3">
        <property type="interactions" value="87"/>
    </dbReference>
    <dbReference type="GeneID" id="8292736"/>
    <dbReference type="KEGG" id="lth:KLTH0F07568g"/>
    <dbReference type="eggNOG" id="KOG1101">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_341677_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKU3"/>
    <dbReference type="OMA" id="QINMENG"/>
    <dbReference type="OrthoDB" id="4070233at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1170.10">
        <property type="entry name" value="Inhibitor Of Apoptosis Protein (2mihbC-IAP-1), Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017956">
        <property type="entry name" value="AT_hook_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051190">
        <property type="entry name" value="Baculoviral_IAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001370">
        <property type="entry name" value="BIR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46771">
        <property type="entry name" value="DETERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46771:SF5">
        <property type="entry name" value="DETERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02178">
        <property type="entry name" value="AT_hook"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00653">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00929">
        <property type="entry name" value="ATHOOK"/>
    </dbReference>
    <dbReference type="SMART" id="SM00384">
        <property type="entry name" value="AT_hook"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00238">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57924">
        <property type="entry name" value="Inhibitor of apoptosis (IAP) repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50143">
        <property type="entry name" value="BIR_REPEAT_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="265"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="414"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="557"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="710"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="288"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="299"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="331"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="566"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="590"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="718"/>
            <end position="731"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR24094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="858" mass="95484" checksum="A7C05B57748AF02F" modified="2009-07-28" version="1">MSSAFHRYRDRHESFCRVFNVQGKRCTWPYRVIPAKAMAQLGFEYQPTFIGEALCRDYVSCCYCGLNTYNFHECRAKALTLTLSKVLEKHLKLSPGCLNSALKLVLMRSYYKAKPIDWSMEAPFSDPHSEDMISLRQWTFNEGWSHSEQPGLSAESMAKAGLLRYDLGLPSSELQEDHNDTTYCVYCSKIIGSWEVGDDPIWEHFVCCNGGNCHFFETMPNQLLLESLKAKLMNSSLSTDVNTPPSLDYYEMFGSLLKRFEEQTTVEESVIKRGRPRSSHINELPHEPRKRGRPKKQRIVGEGDHENSTEGAPQSTEKEVVVKRKRGRPKKIIDESSAEIKPKRPRGRPKKDAPIPITQSIELPKVSEDLLSQGDPEGTNILEPFADKEQSNLSENLPKGSSSLNSDFAQREISAPPFAGKESSTSPRRRIKLRTNSKTPMLAEDELNESDTNSTKSIIVNFKSRSPQKEDTARNPIIDDSFDAFSFSAHGNSEFIIPENAFQLHLNKKGSSAEKFPSTPVKQALISEGSVEMKNPSGSHGKSSSGILDKIDMDVEISDSSDDSPGRNSSKSEGSSPIHTPVHRTEESPGKSSFKGGKFSSAGLLSENASLPTVKPSSVSVNDGEHPFFGVGLLENYASNTKGTNKVLQSPKATTVSSEQHFVNDRVPEKKTATFPIEQAPGSQPFSIGSGSAALPIPNEESTTIFKETLGPEVTTTGEEHSLSKPTERDSGQTGNPSCLEKSLNSIDEDRRVLSHYFHNLLRYINLNDASLANEADGDLSFFVKHMPEDEKRLSFAAWLDEKVVALKEEFNRDYKLKIVKLEEQFQSAQTFVRNLDSEEALLGIAKEYNIDLLSPSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKZ4</accession>
    <name>C5DKZ4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0F08712p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0F08712g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554582.1">
        <property type="nucleotide sequence ID" value="XM_002554536.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKZ4"/>
    <dbReference type="FunCoup" id="C5DKZ4">
        <property type="interactions" value="109"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKZ4"/>
    <dbReference type="GeneID" id="8292788"/>
    <dbReference type="KEGG" id="lth:KLTH0F08712g"/>
    <dbReference type="eggNOG" id="ENOG502S4IW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_983745_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKZ4"/>
    <dbReference type="OMA" id="DEMAINV"/>
    <dbReference type="OrthoDB" id="4070577at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="109"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="154"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="261"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="154"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="170"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR24145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="301" mass="33333" checksum="ECFF862D418CC3BE" modified="2009-07-28" version="1">MAPEDKGSQVKARIDHGDVEQLVKLEPNLNDHELELTLHTLLTQANENPDADDPLHHALEKVISEMELPEMMVGPAAGWGPLEPDQDSVLESPTHLTVENILAGDALHNYGGRGKKRAYDDQENEDETGRDPLCDAHVREPNTALQNVYETLSPESLSPISDSGYKQKSAHPAAPAAVARVDGRPTPKPASAVQSTVPNGTEKKHTSAEPPSKLTNEFTMLQVADVKKRIINTHKLMLNFNFLKDGYARTCVELKRTLLRLKQSEVHRAQLLQENERLKRLVIEQNNKLEGVQATRPASLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>C5DL47</accession>
    <name>C5DL47_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="16">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ordered locus">KLTH0F09922g</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 16">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66132"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:66133"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Membrane</location>
            <topology evidence="1 16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="16"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554635.1">
        <property type="nucleotide sequence ID" value="XM_002554589.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DL47">
        <property type="interactions" value="50"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DL47"/>
    <dbReference type="GeneID" id="8292843"/>
    <dbReference type="KEGG" id="lth:KLTH0F09922g"/>
    <dbReference type="eggNOG" id="KOG0206">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000846_5_3_1"/>
    <dbReference type="InParanoid" id="C5DL47"/>
    <dbReference type="OMA" id="GWFLWNI"/>
    <dbReference type="OrthoDB" id="377733at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140346">
        <property type="term" value="F:phosphatidylserine flippase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006892">
        <property type="term" value="P:post-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000090">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000172">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF174">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE DNF3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 14 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 15 16" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 14 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 16" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="134"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="427"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="473"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1275"/>
            <end position="1295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1325"/>
            <end position="1344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1356"/>
            <end position="1374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1386"/>
            <end position="1410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1430"/>
            <end position="1450"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="19">
        <location>
            <begin position="102"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="20">
        <location>
            <begin position="1212"/>
            <end position="1460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="633"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="841"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="924"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1512"/>
            <end position="1553"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="808"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="641"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="844"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="925"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="13">
        <location>
            <position position="542"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="542"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="542"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="543"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
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            <position position="544"/>
        </location>
        <ligand>
            <name>ATP</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="544"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
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    <feature type="binding site" evidence="14">
        <location>
            <position position="990"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="1070"/>
        </location>
        <ligand>
            <name>ATP</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="1071"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="1072"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="1158"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
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        <location>
            <position position="1164"/>
        </location>
        <ligand>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="1185"/>
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        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
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            <position position="1188"/>
        </location>
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            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="1189"/>
        </location>
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        </ligand>
    </feature>
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            <position position="1189"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-1"/>
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    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-2"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-3"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU362033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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            <dbReference type="Pfam" id="PF16209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF16212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="CAR24198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1613" mass="182520" checksum="8B28FCB90ABF01A2" modified="2009-07-28" version="1">MGRGSRKRAFSLRTQLFNKQLHDKFQRDYEEEPEMGDCGDGKRDNSALELLAGGAQRVTDWVLRRKREKHTKAGRVIPLGLDRLDPFFRTFAGENAELLDERSGRSYCSNLITSSRYTIYSFLPRQLYAQLSRLANTYFLIVAILQMIPGWSTTGTYTTIVPLLIFMSISMAREAWDDFRRHKLDKEENDKLAKMLIMESEKSDHRLRDASEGVTRTNSLYSLPEWQQTATSTSALTNPASEFADEGHEMDTNEREEDSFNNWERLRRRHRIKIIQKKWKDIKVGDYVLLNQDDWIPADLLLLTCDGENNECFVETMALDGETNLKNKQPLTEVSEIMKTTEGLAKFKAKVTVEDPNIDLHNFEGNLELLDENRKLTIGPDHIIYRGSIIRNTQNAVGLVVFTGEETKIRMNAIKNPRIKAPKLQRAINMIVLFMVFVVASMALFSFMGQRIIKKKNVDNGRAWYLFSADAGVAPTVMSFIIMYNTLIPLSLYVTMEIIKAMQSKLMEWDIDMFHAETNTPCESRTATILEELGQVSYIFSDKTGTLTDNKMVFRGLSVCGSSWVHDNDPQEQELDRTESNESKTIDVVSVDDRSFLKNFQNSDGNQVQRQRTSVDFKGNSSAIYTGRPSIASRVQSMRNSMEDSSKVREPSGDGENNKEARSSAELIHYIQQNPDTYFSQKAKFFILSLALCHTCLPKKPSEGGNDDSVEYQASSPDELALVTAARDLGYAVMNKNANVLSIKSYPDGFESEPLIENFEVLNFIEFSSQRKRMSVLVRLPQEPNRILLICKGADNVILERLHNSDLASKKVSELQESTNERKAAEADLVLQQRKSLEIAATGRPSTSLTRSSVNKPTRASLSLQAVRKSLSRRSMGSGDPEVQINSIDDFLSNVERANNAVTEVFAKSRKSMNEQQRERYMPIMQNSPGSASTTNSSGQMPKTPTDVLGSPLQNDIELYIGNENLLQNEEFVLERTLQAIDDFSTNGLRTLLYSYKWIDIEEYEIWNKDYHSAKTSLENRKEKMHSVGEQMETSLRLLGVTAIEDKLQEGVADAIEKIRRAGIKMWMLTGDKRETAINIGYSCNLIHDYSTVVILSAEDENISSKLTAVTQEIDRGNIAHCVVVIDGGTLGNFESNPTLMSVFVELCTKTDSVICCRASPSQKALMVTHIRNTDKKLVTLAVGDGANDIAMIQSADIGVGIAGKEGLQASRSADYSLGQFRFLLKLLLVHGRYNYVRTSKFVLCTFYKELLFYLTQMIYQRHTMFSGTSLYEPWSLSMFNTLFTSLPVLCIGMFEKDLKPMTLLAIPELYSIGRLSESFNLRVFLYWMFLAALNSVIITFLNWKIWAFSSLLDNTIYPIGVINFTAIVTLVNVKCQFLETHNRNILAICSVIISVGGWLLWCCLLPGIYSQDEAYDVLTGLYHQFGRDITFWCACLVLVVLPLIIDVVFKTVKIMIRPSDSEIAMELEQKDEVRKKLEFKAYNELKQGWTWDRDRSTFSRYAGKVFRTGSGTLEPDASGNHTRSRKNTIPSATELPAGSASEVTVRSGTKFDPEEYEILPSGKLIKRCKPASNNGPEDRPLTQKLGKKLRFKIKSKEEDVDAIIADRLRSLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DL60</accession>
    <name>C5DL60_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 15">Dolichyl-phosphate-mannose--protein mannosyltransferase</fullName>
            <ecNumber evidence="4 15">2.4.1.109</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">KLTH0F10252g</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Transfers mannose from Dol-P-mannose to Ser or Thr residues on proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-seryl-[protein] = 3-O-(alpha-D-mannosyl)-L-seryl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17377"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:13546"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137321"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 15">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-threonyl-[protein] = 3-O-(alpha-D-mannosyl)-L-threonyl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53396"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:13547"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137323"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 15">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Endoplasmic reticulum membrane</location>
            <topology evidence="1 15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the glycosyltransferase 39 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.109" evidence="4 15"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554648.1">
        <property type="nucleotide sequence ID" value="XM_002554602.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DL60"/>
    <dbReference type="FunCoup" id="C5DL60">
        <property type="interactions" value="78"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DL60"/>
    <dbReference type="GeneID" id="8292858"/>
    <dbReference type="KEGG" id="lth:KLTH0F10252g"/>
    <dbReference type="eggNOG" id="KOG3359">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008438_2_0_1"/>
    <dbReference type="InParanoid" id="C5DL60"/>
    <dbReference type="OMA" id="KNVTPRL"/>
    <dbReference type="OrthoDB" id="292747at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004169">
        <property type="term" value="F:dolichyl-phosphate-mannose-protein mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd23283">
        <property type="entry name" value="beta-trefoil_MIR_PMT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003342">
        <property type="entry name" value="ArnT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036300">
        <property type="entry name" value="MIR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016093">
        <property type="entry name" value="MIR_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027005">
        <property type="entry name" value="PMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032421">
        <property type="entry name" value="PMT_4TMC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050">
        <property type="entry name" value="DOLICHYL-PHOSPHATE-MANNOSE--PROTEIN MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050:SF50">
        <property type="entry name" value="DOLICHYL-PHOSPHATE-MANNOSE--PROTEIN MANNOSYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02815">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02366">
        <property type="entry name" value="PMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16192">
        <property type="entry name" value="PMT_4TMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00472">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82109">
        <property type="entry name" value="MIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50919">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 15" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="53"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="163"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="226"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="276"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="583"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="628"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="660"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="687"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="17">
        <location>
            <begin position="326"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="17">
        <location>
            <begin position="390"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="17">
        <location>
            <begin position="460"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU367007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAR24211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="835" mass="94226" checksum="6F691EACC64E2705" modified="2009-07-28" version="1">MPPKAYSESQGTGSQDPVPDLNIGKGPERPYVVTEPPSSLVESRTPKSAKEKLTIAVLLLVTAFVRLYGLSYPNSVVFDEVHFGGFASKYIKGVFFMDVHPPLAKMLFAAIGSIAGFTGDFDFESIGQMFPATTPYFFMRLFPAVLGTLTVLLMYMTLRCSGVRVPIAFIASLCFAVENSYVTISRYILLDAPLLFFIASAVYSFKKYEIYPQGSWNSQKALLSTGLALGMALSAKWVGLFTIAWVGILCVWRLWFMIGDLSKPIRKTVREAVRKFSFLLVIPLVIYVFFFYVHFKCLTIHSDGAGFFSSAFRTTLQGNTIPQNILADVGIGSTVSIRHIATMGGYLHSHDHMYEKGSQQQQVTLYPHLDGNNDWLIELHDKPNTPVTSFEGLKDGTIIKLKHVISQRRLHSHDHKAPVSESADWQKEVSCYGFDGFEGDGNDNWAIEIDKDATAPGEAQERVKALETKFRLKHTIMNCHLFSHEVKLPKWGFEQQEVTCASQGKPHLTLWYIEGNSHPLLNETSERVSYKEPTFLEKLVESHQKMWHINKNLNEPHIYESKPHSWPFLLRGINYWGHDHKQVYLLGNAIVWWSVTAFIAIFGLLVGYELLAWQLGKPILQDAKVVNFHVQTIHYLLGYVLHYIPSFLMGRQLFLHHYLAAYYFGILALAHAMEITVTYVFRKRETVGYAIAGAFLSAVVYFFVSYRALIYGTPWTNELCQKSKWLSGWDYPCHNFLSSYEDYKKLETEQAHQPVSPSNTLAAGADSQPTVAQQAPNAQQAIPEDFDVDEIIAAPGNKKFVDQNGNELPFDVVKEVLDNGGSVMSVEHRSHTEIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLP1</accession>
    <name>C5DLP1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0G02310p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0G02310g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555139.1">
        <property type="nucleotide sequence ID" value="XM_002555093.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLP1"/>
    <dbReference type="FunCoup" id="C5DLP1">
        <property type="interactions" value="47"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLP1"/>
    <dbReference type="GeneID" id="8293401"/>
    <dbReference type="KEGG" id="lth:KLTH0G02310g"/>
    <dbReference type="eggNOG" id="ENOG502QTT6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008227_2_0_1"/>
    <dbReference type="InParanoid" id="C5DLP1"/>
    <dbReference type="OMA" id="NRIYHVY"/>
    <dbReference type="OrthoDB" id="413313at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013946">
        <property type="entry name" value="NCA2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28234">
        <property type="entry name" value="NUCLEAR CONTROL OF ATPASE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28234:SF1">
        <property type="entry name" value="NUCLEAR CONTROL OF ATPASE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08637">
        <property type="entry name" value="NCA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="479"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR24702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="605" mass="67968" checksum="AD367CC151B0187A" modified="2009-07-28" version="1">MFTDQFVLDAFEDIDAKLELALQQSSFVGQDHSSSNSDQLKKLQDQLWLVKNEVVALTASLRQRRVRLPNIKLLKSVLESLQEDAPSDFVAVSDNVEGPVRAAIIQYVTVCLYYCVVQKSLDQLPLVHDTAQYYSEIHDSTRWSLLYALQVAPQRLVHEANAVKNDVSRSASWRAMGSLEARSIGTAWYHRLVEQCRKMVMVHNFQLVGVPRDAKKVVSSLWALPLAAVRDEVGTRRQAADTLFNSTASRLGALMTQFPATRDTEARVALLSTFFNHSRASSPGGSEKALLAVMPEVMTLAPASKIQAPGFLTRYWPTLLCSLAYGPSSAFALWQSRDKILQFFQENVVDFCAGLIQNWIWTPLQQVWSTVRHDESQPSIAVASKGSLDSEFSSLTRMVVQLVAENSDGHVNTDALAVQVENGDLTEFMQIYERQIHHPVKNILTGKLVRSLLIQIQKTKVDASLALNGIDKLLSSQQLVFGVVAMSPALLIVYSIWTCIYRLSKLGRIWSNVAQFRFRLMSSMNNIERLLNYNVSEIESSDKDLNTGLLALEVVSARRYGEKLVPKKYRGQWTRDVGELVDPGLNNTARLNVVNRIYHVYGRYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLQ1</accession>
    <name>C5DLQ1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0G02552p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0G02552g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555149.1">
        <property type="nucleotide sequence ID" value="XM_002555103.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLQ1"/>
    <dbReference type="FunCoup" id="C5DLQ1">
        <property type="interactions" value="116"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLQ1"/>
    <dbReference type="GeneID" id="8293412"/>
    <dbReference type="KEGG" id="lth:KLTH0G02552g"/>
    <dbReference type="eggNOG" id="KOG1968">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019060_0_0_1"/>
    <dbReference type="InParanoid" id="C5DLQ1"/>
    <dbReference type="OMA" id="RDINFIP"/>
    <dbReference type="OrthoDB" id="10064318at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23389">
        <property type="entry name" value="CHROMOSOME TRANSMISSION FIDELITY FACTOR 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23389:SF11">
        <property type="entry name" value="TELOMERE LENGTH REGULATION PROTEIN ELG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="797" mass="91383" checksum="6EBCA4676C06BD8A" modified="2009-07-28" version="1">MTSPTPRKTVRLGTLLSGNRKPKIELASDLHSDVSTDDFLDSIETSGIIDYDNNWTTGDSSPLRIELNNQKISCATDDVHLPQREPPHRTSLPFVGDCEIIAEPGTPKLTVKQILSGKSQPGLGNKKTQPSSKTSCSKSEDLEVIHISDSGKNRETHERIMSGLTCTKKTSVKKLFDNFQKRNRNQEEYQEIDEVELVSTTPVKPLHEVSSLDSMETPLPASQNVTDNTDYINARPLSLKKKNKSAPELVLNFDPHEYKSLNLSNKQSKTALQTLGLEASGRRCSNKIWTEDFEPHDVENVILNDNLKRDVHSWLDKAFKKLRKKTTRNKLRKKKVEADDEMDFFVVDDDLEEGDEQEEEFVPLMILSGEAVGKTTLLKSLMNSYSGQIFEINASGNRGKRDFLQSVLDFSTTHYVKDKGSKGLILFDDVDVLFRERDKLFWNAVEKLLIKARRPVVLICRDINYIPLNILQVAQEENSLFQAKAIDFPKAVDQLQQFLTSRGLLWPREFLEHIIKLNMCDIRKCLMELQWMSLSDHTSERTVEPVVPPQDIASALFSSTLLSEQDVILRGIQWKSNLEQDRDSTVYFASKSKDFVSFSDEQKLAFDYMVDYKQHLHDSLKRPLMPFEMDVTEYLRSRVFDPNEQQECGNHIFQKAAMETIHYLKSRVSETASFNEDHGGNARMTRNSRKVKEIMDRFSENGDHVRSSFNDYFMNLTSVMTRSQICQDVIPLVCEVAKHDKARKAQNRLIYQNAMTGVPPENEREVVIRLLQEHAFYPIWFKGEPDLLLSAWSFRPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DM45</accession>
    <name>C5DM45_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Mediator of RNA polymerase II transcription subunit 6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 11">Mediator complex subunit 6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0G05896g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 11">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the Mediator complex subunit 6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555293.1">
        <property type="nucleotide sequence ID" value="XM_002555247.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DM45"/>
    <dbReference type="FunCoup" id="C5DM45">
        <property type="interactions" value="894"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DM45"/>
    <dbReference type="GeneID" id="8293561"/>
    <dbReference type="KEGG" id="lth:KLTH0G05896g"/>
    <dbReference type="eggNOG" id="KOG3169">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077754_0_0_1"/>
    <dbReference type="InParanoid" id="C5DM45"/>
    <dbReference type="OMA" id="MQRQFSQ"/>
    <dbReference type="OrthoDB" id="344220at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.580:FF:000004">
        <property type="entry name" value="Mediator of RNA polymerase II transcription subunit 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.580">
        <property type="entry name" value="Mediator complex, subunit Med6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007018">
        <property type="entry name" value="Mediator_Med6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016612">
        <property type="entry name" value="Mediator_Med6_fun"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038566">
        <property type="entry name" value="Mediator_Med6_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13104">
        <property type="entry name" value="MED-6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04934">
        <property type="entry name" value="Med6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF013286">
        <property type="entry name" value="MED6_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0010">Activator</keyword>
    <keyword evidence="8 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 11" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="178"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="181"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR013286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR24856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="257" mass="28319" checksum="0943677097EE34F2" modified="2009-07-28" version="1">MSAPQLDELQWKSPEWIQSFGLRTDNVLDYFAESPFFDRTSNNQVAKMQQQFAQPRPAGQVAPSMSLEDPDPHRRSILSTYMVHALWEQELRKLKGVEYILAYVREPDFWIIRKQNRTGPTAVSPLQDYYIIGANVYQAPSVFKIVQSRLLSSNLHLSKALSSLRRMTEFQPSQGGHFVKTSYQATPSSSQQPMTVASTSNAANSAANTGPATANGQSGLTGTADPIGLAGQPSATMNQAQMDKLLATSMKSSPAYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>C5DME0</accession>
    <name>C5DME0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0G08074p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0G08074g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555388.1">
        <property type="nucleotide sequence ID" value="XM_002555342.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DME0"/>
    <dbReference type="FunCoup" id="C5DME0">
        <property type="interactions" value="110"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DME0"/>
    <dbReference type="GeneID" id="8293659"/>
    <dbReference type="KEGG" id="lth:KLTH0G08074g"/>
    <dbReference type="eggNOG" id="KOG0071">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040729_9_3_1"/>
    <dbReference type="InParanoid" id="C5DME0"/>
    <dbReference type="OMA" id="GGQISKM"/>
    <dbReference type="OrthoDB" id="2011769at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030010">
        <property type="term" value="P:establishment of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000412">
        <property type="entry name" value="ADP-ribosylation factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="24"/>
            <end position="31"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="126"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR24951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="184" mass="20937" checksum="BCB5542041F500C6" modified="2009-07-28" version="1">MGNSISRVMVKMFGYREMKILMLGLDNAGKTTILYKLKLNKIKTSAPTVGFNVETVSFRNVKFNMWDVGGQDRLRPLWRHYFPATTALIFVIDSQDMKRLNEAKEELYSIIGDKEMEEVVLLVLANKQDLKGSMNPQEISDYLELKSHLDSQLWCVIGSNALTGQGLVEGLSWITNNTKTKDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMF7</accession>
    <name>C5DMF7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0G08514p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0G08514g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555405.1">
        <property type="nucleotide sequence ID" value="XM_002555359.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMF7"/>
    <dbReference type="FunCoup" id="C5DMF7">
        <property type="interactions" value="45"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMF7"/>
    <dbReference type="GeneID" id="8293679"/>
    <dbReference type="KEGG" id="lth:KLTH0G08514g"/>
    <dbReference type="eggNOG" id="ENOG502QURR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017550_1_0_1"/>
    <dbReference type="InParanoid" id="C5DMF7"/>
    <dbReference type="OMA" id="ATRGEWF"/>
    <dbReference type="OrthoDB" id="41532at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006644">
        <property type="entry name" value="Cadg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05345">
        <property type="entry name" value="He_PIG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00736">
        <property type="entry name" value="CADG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950544" evidence="3">
        <location>
            <begin position="19"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="485"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="Dystroglycan-type cadherin-like" evidence="4">
        <location>
            <begin position="21"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Dystroglycan-type cadherin-like" evidence="4">
        <location>
            <begin position="142"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="Dystroglycan-type cadherin-like" evidence="4">
        <location>
            <begin position="341"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="439"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="515"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="572"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="678"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="803"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="439"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="516"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="713"/>
            <end position="725"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR24968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="824" mass="88162" checksum="4EDC544F99932B7E" modified="2009-07-28" version="1">MRGAWVLLVGFVASLVRTQPYEAYAASKQLPPVAIAGEAFSFKLSNDTFKSSVDEATQIQYEAYGLPSWLEFDSANRVLSGTPAQDDLGQDSGTSYVSYTLQGIDPADNGTLNKTYQLALSDGTQPAVAPSFNLLSLLKNSGYTNGKNALKLSPGEIFNVSFDRDTFTNSSSATTFYGRSALYHAPLPSWIFFDESSLKFSGTAPVVNSEIAPESYFSFSLIASDVTGYTSSEVMFDLVVGAHQFTTSIQNTLVVNVSSSGSFNYKLPMNYVYLDDEPVSQSNISSIQLLDAPDWASVSNQSLSGTLLTGESSENLTIAIEDVYGDAVYLNFLVESTSKLFAVSSLPNVNATKDEWFQYSLLPSQFTDYDNTNVSVSFSNSSDSSSWLSFHSSNLTLMGKVPDDFTSLAVDVSAKQSSDEQKLSFKVLGVSSLKKTSSSTSAQSSKTATSSATSSASSPTATSSQTQAASPTNETQKKKSNNRTTAIACGVAIPVAVILALILFLLLWRRKKNKSGAAQDQENTPRIGNPQLGNPANNPNVIGAVSPFGDENSLDDTSTAKRLAALNGMKLDAQGGSGSEGSTIDEKAECGSLSSSSSDSYHDAAFTASTDALVPRDESDQYFDSNKHTSSVYLNSEPTNRKSWRFSAAGAAANRNTRESYNSLNTVSTAELFSTQIAGNKSLPKDPRKSSLGMRDSVFMGNLSNPARGTRDGSSSNSGSLSASNVAKSSEGLKESTLPHLKEIPHDENSFHSQITASSSDDFIPVKDGNNYTWIERINNAEVKTPERKRSLKRLVSLPNKSGVGVCDANDIQGQEPEVDASMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMG6</accession>
    <name>C5DMG6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0G08756p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0G08756g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555414.1">
        <property type="nucleotide sequence ID" value="XM_002555368.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMG6"/>
    <dbReference type="FunCoup" id="C5DMG6">
        <property type="interactions" value="536"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMG6"/>
    <dbReference type="GeneID" id="8293690"/>
    <dbReference type="KEGG" id="lth:KLTH0G08756g"/>
    <dbReference type="eggNOG" id="KOG0712">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017633_10_0_1"/>
    <dbReference type="InParanoid" id="C5DMG6"/>
    <dbReference type="OMA" id="RGPDIKH"/>
    <dbReference type="OrthoDB" id="550424at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072655">
        <property type="term" value="P:establishment of protein localization to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10747">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10719">
        <property type="entry name" value="DnaJ_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.230.10:FF:000001">
        <property type="entry name" value="DnaJ subfamily A member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.230.10">
        <property type="entry name" value="Heat shock protein DnaJ, cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.20">
        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044713">
        <property type="entry name" value="DNJA1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001305">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43888">
        <property type="entry name" value="DNAJ-LIKE-2, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00684">
        <property type="entry name" value="DnaJ_CXXCXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="DnaJ/Hsp40 cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51188">
        <property type="entry name" value="ZF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="1 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="J" evidence="8">
        <location>
            <begin position="8"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="CR-type" evidence="9">
        <location>
            <begin position="155"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CR-type" evidence="6">
        <location>
            <begin position="155"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="421"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="444"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR24977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="462" mass="51048" checksum="106DF7E1CC246608" modified="2009-07-28" version="1">MKSVSNSELYDILGVSASASRQDIKKAYRVNALKHHPDKNGHSEESKLKFQQICKAYEILKDERKREMYDRFGTADESQWHTESTSYQEQSGMSAGDLFAQFFGGGSTTGSFFNDDMSFFGKRQGAHTAPSRRELPRGPDIKHYLKCTLEEVYHGKRAKLALKRTRLCKKCKGEGGLKASQCYTCGGRGIRTETKRHGPMVQTWSSTCSDCAGRGSFMKQKDICRDCNGQGCIRERRIFDIEVHKGMENGQEVVLPGEADEVVGSELGSEHVIPGDVIIILEQLPHKAFWRHRDASLVLDHCKVDLKTSLCGGPVWVDSHPSGKLLKIDVLPGEILKPGAIKCVEGMGMPKSGGRFGNLYIRFDVAFPESLKPETVEALRAALGQEPGANSGAGEPAVAVEGREVEEHVLSNFAPDLELSRDSYRKRQARGQSASSKRRKYADDDSNDSDVADEQNGGCTVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMI8</accession>
    <name>C5DMI8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0G09284p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0G09284g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555436.1">
        <property type="nucleotide sequence ID" value="XM_002555390.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMI8"/>
    <dbReference type="FunCoup" id="C5DMI8">
        <property type="interactions" value="280"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMI8"/>
    <dbReference type="GeneID" id="8293713"/>
    <dbReference type="KEGG" id="lth:KLTH0G09284g"/>
    <dbReference type="eggNOG" id="KOG1209">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_2_9_1"/>
    <dbReference type="InParanoid" id="C5DMI8"/>
    <dbReference type="OMA" id="CRVFASD"/>
    <dbReference type="OrthoDB" id="2102561at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000140">
        <property type="term" value="F:acylglycerone-phosphate reductase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019433">
        <property type="term" value="P:triglyceride catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05374">
        <property type="entry name" value="17beta-HSD-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44169">
        <property type="entry name" value="NADPH-DEPENDENT 1-ACYLDIHYDROXYACETONE PHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44169:SF6">
        <property type="entry name" value="NADPH-DEPENDENT 1-ACYLDIHYDROXYACETONE PHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR24999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="288" mass="31877" checksum="6DEFC08436A43295" modified="2009-07-28" version="1">MAEGKIALVTGASSGIGYELTRQLAGKGYKVYAAARREERIAPLQKEFPQLVVPIKLDVSEPEQISALRDRLAKELPSQKLDILYNNAGQSCTFPASDVTNDVLEQAFKVNVFGPINTCRELLPFVINAQGTVIFTGSLAGIISFPFGSVYSATKGAIHSYARGLHIEMKPFGVRVLNVITGGVGTDIADKRPLPEDSIYNIPEASEAMAYRREMAKNNKPMDVGKYVSSVVRDIESSRDPVDIYRGTFAGAARWISLLMPYWMLDWILARRFKLTGMINALQKRKRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMP4</accession>
    <name>C5DMP4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA polymerase II holoenzyme cyclin-like subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0G10560g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclin family. Cyclin C subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555492.1">
        <property type="nucleotide sequence ID" value="XM_002555446.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMP4"/>
    <dbReference type="FunCoup" id="C5DMP4">
        <property type="interactions" value="1042"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMP4"/>
    <dbReference type="GeneID" id="8294191"/>
    <dbReference type="KEGG" id="lth:KLTH0G10560g"/>
    <dbReference type="eggNOG" id="KOG0794">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034754_2_1_1"/>
    <dbReference type="InParanoid" id="C5DMP4"/>
    <dbReference type="OMA" id="CLLHPPH"/>
    <dbReference type="OrthoDB" id="10266018at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20513">
        <property type="entry name" value="CYCLIN_CCNC_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20546">
        <property type="entry name" value="CYCLIN_SpCG1C_ScCTK2-like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000077">
        <property type="entry name" value="RNA polymerase II holoenzyme cyclin-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043198">
        <property type="entry name" value="Cyclin/Ssn8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031658">
        <property type="entry name" value="Cyclin_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10026">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16899">
        <property type="entry name" value="Cyclin_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028758">
        <property type="entry name" value="Cyclin, C/H/G types"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="6 10" id="KW-0195">Cyclin</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Cyclin-like" evidence="11">
        <location>
            <begin position="79"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR25055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="299" mass="35269" checksum="CCDBDC4973657672" modified="2009-07-28" version="1">MSASYWESTQRQKWQYTRESLFRERHQLWLMECQLFPQGLTVTMENNKADGEAVTRNIPITHVDLHYGKDYNLRIYCYFLIMKLGRRLNIRQYALATAHVYLARFLLCASVREVNLYLLVTTCIYLACKVEECPQHIRALVNEARSLWPEFVPPDPTKVTEFEFYLLEELQSYLIVHHPYRSMEQIVSALRSEPYGLVLSATDLQNCWSLINDSYITDLHLLVPPHVIAMASMLIIVSLQRSQRPSLQETFNSFMAQSQVDLAEVMDTIQDLITMYDYWDKYNEPWVKFLLHSLYLRST</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMX3</accession>
    <name>C5DMX3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">KLTH0G12386p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0G12386g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555571.1">
        <property type="nucleotide sequence ID" value="XM_002555525.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMX3"/>
    <dbReference type="FunCoup" id="C5DMX3">
        <property type="interactions" value="407"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMX3"/>
    <dbReference type="MEROPS" id="A01.030"/>
    <dbReference type="GeneID" id="8293853"/>
    <dbReference type="KEGG" id="lth:KLTH0G12386g"/>
    <dbReference type="eggNOG" id="KOG1339">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013253_9_1_1"/>
    <dbReference type="InParanoid" id="C5DMX3"/>
    <dbReference type="OMA" id="QFGCNST"/>
    <dbReference type="OrthoDB" id="771136at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05474">
        <property type="entry name" value="SAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000023">
        <property type="entry name" value="Aspartic protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033876">
        <property type="entry name" value="SAP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF65">
        <property type="entry name" value="ASPARTIC-TYPE ENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 8" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002948843" evidence="10">
        <location>
            <begin position="19"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="11">
        <location>
            <begin position="79"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="528"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="97"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="371"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="406"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR25134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="580" mass="60063" checksum="7002559DB9BBEBEC" modified="2009-07-28" version="1">MKLLSTSTFLAACGLASAAVVSSRDEGAQSASKFVKVSFDKLRGDTAEDATAGAVAHGVLAKRADGSETVVIKNQQTFYSVELGVGSPAQNVTLLIDTGSSDMWVMGSNNPYCEGGSSGNSKHVLLGDHVDSRDEEVNRDWFSTLRSFLTATATATATATATATGSADSGGNAPSRTSVNSQYATLTCSSYGTFATDSSSSFQSNNTAFYIEYGDSTYALGTWGRDNVTLQDITLSGISMAVANESNSTFGVLGIGLAALEVTNVGTLATRGNSYTYENLPAKMVSEGIIHKNAYSLFLDSLDAKEGSVLFGAVDHSKYTGSLYTLPLSNSYKSMGYSDPIQLEVTLQGFGMTANNTNSTFTTTPLTALLDSGTTLTYLPTTLVRQVAAKYGARYDSSYGYYLLSCSDVKDTDELVFDFGGFHINSKVENFLIRATSNICALGLIPQSSSSLILGDNFLTAAYVVYDLDNLEISLAQANYDGGSSNIEVISSSVPSAVKGPSYSSTWSTSVPISTGGNIFTVSGSNSTTTAGNSASGTTSSSRSSSTSSAGKKNAAVGAASPGVSGLLGALVLFIAAGLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>C5DN01</accession>
    <name>C5DN01_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0G13068p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0G13068g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the WD repeat WDR59 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555599.1">
        <property type="nucleotide sequence ID" value="XM_002555553.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DN01"/>
    <dbReference type="FunCoup" id="C5DN01">
        <property type="interactions" value="405"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DN01"/>
    <dbReference type="GeneID" id="8293882"/>
    <dbReference type="KEGG" id="lth:KLTH0G13068g"/>
    <dbReference type="eggNOG" id="KOG0309">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001497_0_0_1"/>
    <dbReference type="InParanoid" id="C5DN01"/>
    <dbReference type="OMA" id="HRRETCL"/>
    <dbReference type="OrthoDB" id="311712at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035859">
        <property type="term" value="C:Seh1-associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035591">
        <property type="term" value="F:signaling adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16488">
        <property type="entry name" value="mRING-H2-C3H3C2_Mio-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006575">
        <property type="entry name" value="RWD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049567">
        <property type="entry name" value="WDR59-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049566">
        <property type="entry name" value="WDR59_RTC1-like_RING_Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46170">
        <property type="entry name" value="GATOR COMPLEX PROTEIN WDR59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46170:SF1">
        <property type="entry name" value="GATOR COMPLEX PROTEIN WDR59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05773">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17120">
        <property type="entry name" value="zf-RING_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00591">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50908">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0926">Vacuole</keyword>
    <keyword evidence="3 6" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="110"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="197"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="RWD" evidence="8">
        <location>
            <begin position="433"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="688"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="688"/>
            <end position="702"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR25162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1101" mass="124591" checksum="30BD3DD0CDFCA232" modified="2009-07-28" version="1">MSADNEDDPFKSITFGKSLSLRVDGGFNAVSISPSGRDVVLASRQGLYVVDLDDPFSAPRWLHHITPWEVADVQWSPHPSKPSWVVSTSNQKALVWNLSRSSSNAVEHVLHGHSRAITDINFHPTNPEILATCSIDAYVHAWDMRSSRRAFYSASVWSAGAAQVKWNFKNPNVMASAHSNDIFIWDLRKGCMPLHILKGHANSVNSIDFSRFNETEIMSSSNDGTVKFWDYSVNDKEPLRTVVTDFPVSKGRYLPFGRGFCVMPMTGGNNSVYLSSSTTSNDSGPISKFQPIYVFKGHADRVSDFLWRQRHSHDTLIDDREFQLVTWSKDCDLRLWPVNESTYEKVGYERLRALEKPLPDHEYCSFRKEPEAAKLSTAPTARWKENFVTKTGTEASNGSNNINHLAWVSGVRLHHANSPHDLFEEDKLQNLGEEVSMIGHKFPKIVFEKISVSTGILVLTLNGPWSEENPDDYIFLRLEVKFPSGYPQKKHVPRFKIEENRELSEKKHQEIVDQLGEIARRYTDLGKYCLEPCLRFLLGEKINLDGLQEEEQLLNLDLVDHVELDEFSSLASSDARSRALSDISNTSEDDFDDIDNKSSADGFVTRSTHDLRLDTTPIPKGCGAVWTAAGQLVCFFMVENKVDKKQSFFKLGQRSFGGLKDAHKPELLFEEQDANYDRPKRYVDTLSASSHHNSEGVNSLNASDSDASSDSFGDDWNDIVGNDITWRTKMPLISNFKKSLASMASDDNKSSASVQKSKNIVTVRDFSHLIPDKKQLAMDYKLYGDSLESVCKYNAQVAVTHGFEEIAHCWRMIANVLMAREEKNPYNFGWDQHSLGGKWFVKQTMIHFEKLRNVQMLAILSCILMDQPLGKTVTAPTGPKKREETIITFESNCIDHSQRSRHESVTSSIRSEDYFNGAHGGKTLKPWTPVPVSSQDNLSSHNMLPDIKIEIVNDEVLDLLTSDSPPLLDAEDEAKYRSYRHQYAELLFYWGLLINRTELLKFNFDDDNFTSSMWPLLNHAPQPGFQLDYMGICYKLLEQKGVGTYFSRNCNYCELRVDGRGSVCGNCQHILHAECAKVWWEVSNECASGCGCACVEFFDVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>C5DN17</accession>
    <name>C5DN17_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 9">tRNA (guanine(26)-N(2))-dimethyltransferase</fullName>
            <ecNumber evidence="7 9">2.1.1.216</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0G13420g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>guanosine(26) in tRNA + 2 S-adenosyl-L-methionine = N(2)-dimethylguanosine(26) in tRNA + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:43140"/>
            <dbReference type="Rhea" id="RHEA-COMP:10359"/>
            <dbReference type="Rhea" id="RHEA-COMP:10360"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74513"/>
            <dbReference type="EC" id="2.1.1.216"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the class I-like SAM-binding methyltransferase superfamily. Trm1 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.216" evidence="7 9"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555615.1">
        <property type="nucleotide sequence ID" value="XM_002555569.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DN17"/>
    <dbReference type="FunCoup" id="C5DN17">
        <property type="interactions" value="1283"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DN17"/>
    <dbReference type="GeneID" id="8293898"/>
    <dbReference type="KEGG" id="lth:KLTH0G13420g"/>
    <dbReference type="eggNOG" id="KOG1253">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010862_4_1_1"/>
    <dbReference type="InParanoid" id="C5DN17"/>
    <dbReference type="OMA" id="MKCCHEM"/>
    <dbReference type="OrthoDB" id="6349953at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160104">
        <property type="term" value="F:tRNA (guanine(26)-N2)-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002940">
        <property type="term" value="P:tRNA N2-guanine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.56.70:FF:000001">
        <property type="entry name" value="tRNA (guanine(26)-N(2))-dimethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.70">
        <property type="entry name" value="N2,N2-dimethylguanosine tRNA methyltransferase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002905">
        <property type="entry name" value="Trm1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042296">
        <property type="entry name" value="tRNA_met_Trm1_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00308">
        <property type="entry name" value="TRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10631">
        <property type="entry name" value="N 2 ,N 2 -DIMETHYLGUANOSINE TRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10631:SF3">
        <property type="entry name" value="TRNA (GUANINE(26)-N(2))-DIMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02005">
        <property type="entry name" value="TRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51626">
        <property type="entry name" value="SAM_MT_TRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 9" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="1 9" id="KW-0820">tRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="71"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="71"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00051897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR25178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="542" mass="60748" checksum="A5B0F79915DC1BD5" modified="2009-07-28" version="1">MIRAALAKVRSRFVKPAPKPDASKFTVVTEGKAHILFPEKETVFYNPIQQFNRDLSVSCIKAWDNLYGQAKQEKSRGKGKKRGLEHGEEASKKRKLEDGSSAEVKASSSRPYIKILEALSATGLRAIRYAHEIPNVKEVVANDLLPEAVDAIQRNVDYNKVGHIVKPNIDDANVLMYRNKAAHKRYHVIDLDPYGTATPFIDAALQNIEDDGLMLVTCTDLSVLAGNGYPEKCFALYGGVNMAGHDATHESALRLVLNLLGQSAAKYKKSIEPLLSLSIDFYVRVFVKVKTNPTKVKDLQHNTMITYVCSGCRSYHSQRLGKQTERESKKGKPYLKYSLAQGPPVDKKCHYCGGAHHLTGPMYAGPLHNREFIDEVLRINRDVHGDDVYKTRKRIEGMLTLAKNEVDAPFYFTPNRVSSILKFQVPSLKTVVAGLGSLGYECSLTHAQPSSLKTDAPWGAIWYVMKRYCLDNGLCNVEKMNQNSWGYKILSNDLVAEAAQYGDQLSFEPNDQSGRVEKLRKLKIVRYQENPTKNWGPKARPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>C5DN35</accession>
    <name>C5DN35_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0G13816p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0G13816g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555633.1">
        <property type="nucleotide sequence ID" value="XM_002555587.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DN35"/>
    <dbReference type="STRING" id="559295.C5DN35"/>
    <dbReference type="GeneID" id="8293916"/>
    <dbReference type="KEGG" id="lth:KLTH0G13816g"/>
    <dbReference type="eggNOG" id="ENOG502S3WI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_347781_0_0_1"/>
    <dbReference type="InParanoid" id="C5DN35"/>
    <dbReference type="OMA" id="VWACNNL"/>
    <dbReference type="OrthoDB" id="4064898at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="CDD" id="cd14686">
        <property type="entry name" value="bZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022210">
        <property type="entry name" value="TF_GCR1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12550">
        <property type="entry name" value="GCR1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription activator GCR1-like" evidence="3">
        <location>
            <begin position="542"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="242"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="339"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR25196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="655" mass="72471" checksum="3E7D0F1DD78680EE" modified="2009-07-28" version="1">MEEEPQTRRAAQRASPLVGALERGYTTSGAEGHPVTDPSGAPSGQSLSDSPGTRRSSQAAELTNSVDSTLNLLQDVLWNVLVHIRGHGTFESCYSLAKRRYEAHYQSLVSVECIFKTLANYPAEILSAMYIPVILRFFKWCSSVRQLPPEFKGSRFLITTKKGITFLMTNLSECSYNAATLYQAFRALKLLQSLEIALFPQKPALYHDPEDEEADDPNWKPLDDSQLEVLISSVTKGDFRIGASAPPQPEPTNAGMFEGSALSHAGQSPNPPAPFPVTQQPPQQAAFYGAAQQPPFGGLVPMPQDPYAPLFSGHGNDNFMYQNPEMLFSHTIPSIFHRMNALEAANQELSRENDSLSQSLNDLKSELQSFKRNVDTAIPGVQILTSPRATHSRYPSPTPPNIILSSSNAIDTSDIAASVQKLDTDLCNSSATQTRSLISNYENMVPYGAAPGYIPDAELKSLERLKQEDALVSLSGTGGSIVTPSSGIFLTTAELQSPAKRPKHRNRRIEGAKLSKGETYQPPQAPNGKNYLLDNEGRLAIVMATEQSSVYGVYNEYYQSLRPQVDSYADDFGKKSMGHFRKKRTFQKKKAFVQWVERISQLNNMPPELVLDIVDEVRLKEKKSVVWACNNLGRFRLVLNRYKPDLKDTALSDVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DN70</accession>
    <name>C5DN70_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Pre-mRNA-splicing factor SLU7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0G14630g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in pre-mRNA splicing.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Associated with the spliceosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the SLU7 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555668.1">
        <property type="nucleotide sequence ID" value="XM_002555622.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DN70"/>
    <dbReference type="FunCoup" id="C5DN70">
        <property type="interactions" value="559"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DN70"/>
    <dbReference type="GeneID" id="8293953"/>
    <dbReference type="KEGG" id="lth:KLTH0G14630g"/>
    <dbReference type="eggNOG" id="KOG2560">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072877_0_0_1"/>
    <dbReference type="InParanoid" id="C5DN70"/>
    <dbReference type="OMA" id="KSKMFRR"/>
    <dbReference type="OrthoDB" id="249612at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030628">
        <property type="term" value="F:pre-mRNA 3'-splice site binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021715">
        <property type="entry name" value="Slu7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039974">
        <property type="entry name" value="Splicing_factor_SLU7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12942:SF2">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR SLU7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12942">
        <property type="entry name" value="STEP II SPLICING FACTOR SLU7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11708">
        <property type="entry name" value="Slu7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 8" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5 8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="10">
        <location>
            <begin position="81"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="30"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="298"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="298"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR25231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="338" mass="38671" checksum="BF4BD8A2CE55BD12" modified="2009-07-28" version="1">MSKRQENVHIPKYIKDQPWFYKSSAEGSAENEDYLAHHRRHGRPDSDLDIDNNAEPKIGRGIRDEYEQYTGVSRPRFAKAKCLNCGAADHISRDCLEAPRKRKAYTEAQTRTAVVRRKEIDGNWDARRDRWFGYEGKEYENVLQKWENSAAKQRDQAAGDGDIADELDTDEEIELAALGLFKDEATGAVAADDEQGSKLRASVRLREDRAAYLNDINSETINYDPKSRLYKSDTLGEVDSESKMFHRHLTGESLELSKLSRFAREKTLESGVRDEMEDEAKTRHVLVANPTKYELMMKKEESSVPVKPEDLYRGQSAKKLTGTKQSEDQKAQLLKKYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5DND2</accession>
    <name>C5DND2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0G16016p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0G16016g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MDM20/NAA25 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555730.1">
        <property type="nucleotide sequence ID" value="XM_002555684.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DND2"/>
    <dbReference type="FunCoup" id="C5DND2">
        <property type="interactions" value="75"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DND2"/>
    <dbReference type="GeneID" id="8294016"/>
    <dbReference type="KEGG" id="lth:KLTH0G16016g"/>
    <dbReference type="eggNOG" id="KOG2053">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019572_0_0_1"/>
    <dbReference type="InParanoid" id="C5DND2"/>
    <dbReference type="OMA" id="LPQLAYK"/>
    <dbReference type="OrthoDB" id="1874341at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031416">
        <property type="term" value="C:NatB complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019183">
        <property type="entry name" value="NAA25_NatB_aux_su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22767:SF3">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 25, NATB AUXILIARY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22767">
        <property type="entry name" value="N-TERMINAL ACETYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09797">
        <property type="entry name" value="NatB_MDM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR25293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="778" mass="89423" checksum="7090C36264A14AAF" modified="2009-07-28" version="1">MESTGDEIYKLLSRGNFKQALQLVAKLSTQYPSASYPKVLTQYVKFRQNKQKFDFFTGLEPLLKAKSPPSDSRTLSLLHTFLLELGRFDEVLNVYGAAMQKYPNFETGSNWFSRAIEDMNLRHMVQSSFQLRRLSNDLRTLQFWNAMASVALTKLHGESLSERERELNAALSFKSVSSLAPFQSDQETIVFCHVCEQFDNKSREIVDTLLPSFLKGGKDFSVDLYLKNFLIKHLKVLNDNENMFKCCKLLLEYLDDFEILSHLVASARALNVSKEEVRKHLQVRDSRNHRLAHLELDVIYSQSISDETLRFYLERYHDKPCCVPDLSFYRKSIPDGLIERGMTQLPDSLMHECNAIKLTQATSPDLFLELFKKYKQTLRKKPKTDYSPCSSFVLKIVESLINDKNVNLSNVVTSVLILESYQDEDPHNFDTRIWLVVLYNYLGCPTIAYNHYKELHVKNLQNDTLDHIVASRFSSLLPFKDHPFVEQLSSGDKVYESLINLPQFIRISFEKKSYSKILGMLELNDKLNRSSARWSKIGEQLQHARLFNDKRGELLAKLHESWRHFCLYNVNASGEKCVKSLHLSDNRDFAVLGSLRDSCTTVTGYMDQDEGAILAGCLQEMMIEFFSMGERDPTIDKLLDIRNYTASMTATERWAFETIRLLYQHSPDQGQAALAEQIKAMPKLSSQGWLLTHDYNCQLVTLKTLDNLKRIKNQECKKSIKTQLRGLRDSCKDVFQSYIAEISSCEPNTEILNALGYADAEHHIAKGVLAVLKSSCNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>C5DND6</accession>
    <name>C5DND6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0G16104p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0G16104g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555734.1">
        <property type="nucleotide sequence ID" value="XM_002555688.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DND6"/>
    <dbReference type="GeneID" id="8294020"/>
    <dbReference type="KEGG" id="lth:KLTH0G16104g"/>
    <dbReference type="eggNOG" id="ENOG502S7RD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078289_1_1_1"/>
    <dbReference type="InParanoid" id="C5DND6"/>
    <dbReference type="OMA" id="YGQSQRQ"/>
    <dbReference type="OrthoDB" id="4035853at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020999">
        <property type="entry name" value="Chitin_synth_reg_RCR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28187">
        <property type="entry name" value="PROTEIN RCR1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28187:SF1">
        <property type="entry name" value="PROTEIN RCR1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12273">
        <property type="entry name" value="RCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR25297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="171" mass="19036" checksum="80C6C6A8E92DA85D" modified="2009-07-28" version="1">MPAILAKRIYYYDTSDSSAWVWARWLLFIIFLVVVVLYVFIINTRRRRSGRDPIRGTAWLAPPPSYGISQNHYNQPTNQNPVPVYTENANANDAGYFDQQGKFHPTGAPVVADDVPQPEPAVTRDDRHSLTTSSSSSIEYQRPQVPPPTETAYQRPPGPPPSAAEPGASKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNN2</accession>
    <name>C5DNN2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Serine hydroxymethyltransferase</fullName>
            <ecNumber evidence="10">2.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0G18502g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 10">Interconversion of serine and glycine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O = (6S)-5,6,7,8-tetrahydrofolate + L-serine</text>
            <dbReference type="Rhea" id="RHEA:15481"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="EC" id="2.1.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 9 10">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 10">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 10">Belongs to the SHMT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.1" evidence="10"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555830.1">
        <property type="nucleotide sequence ID" value="XM_002555784.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNN2"/>
    <dbReference type="FunCoup" id="C5DNN2">
        <property type="interactions" value="946"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNN2"/>
    <dbReference type="GeneID" id="8294125"/>
    <dbReference type="KEGG" id="lth:KLTH0G18502g"/>
    <dbReference type="eggNOG" id="KOG2467">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022477_0_1_1"/>
    <dbReference type="InParanoid" id="C5DNN2"/>
    <dbReference type="OMA" id="CQFANVQ"/>
    <dbReference type="OrthoDB" id="10265628at2759"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004372">
        <property type="term" value="F:glycine hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019264">
        <property type="term" value="P:glycine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00378">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000097">
        <property type="entry name" value="Serine hydroxymethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00051">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001085">
        <property type="entry name" value="Ser_HO-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049943">
        <property type="entry name" value="Ser_HO-MeTrfase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019798">
        <property type="entry name" value="Ser_HO-MeTrfase_PLP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039429">
        <property type="entry name" value="SHMT-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000586">
        <property type="entry name" value="PRK00011.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680:SF28">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00464">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000412">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00096">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="8 9 10" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Serine hydroxymethyltransferase-like" evidence="11">
        <location>
            <begin position="17"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="9">
        <location>
            <position position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000412-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR25393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="469" mass="52112" checksum="284E1063942B63DD" modified="2009-07-28" version="1">MPYALSDSHKQLVSAHLSESDPEVEQIIKDEIDRQKHSIVLIASENFTSTSVFDALGTPMCNKYSEGYPGARYYGGNEHIDRMELLCQKRALEAFHVSPEKWGVNVQTLSGSPANLQVYQALMKPHERLMGLFLPDGGHLSHGYQTDTRKISAVSTYFESFPYRVDPATGIIDYDTLEKNAILYRPKILVAGTSAYCRLIDYKRMREIADKVGAYLMVDMAHISGLIAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRRGVRSVNPKTGKEVLYDLENPINFSVFPGHQGGPHNHTISALATALKQAATPEFKEYQDQVLKNAKALESQFKKLGYRLVSDGTDSHMVLVSLREKGVDGARVEYVCEKINIALNKNSIPGDKSALVPGGVRIGAPAMTTRGLGEQDFVKIVDYIDKAVQIAHDVQHSLPKEANRLKDFKAKVDQNIQDLEPIKKEIYSWAGEFPLPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNP7</accession>
    <name>C5DNP7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Actin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0G18832g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the actin family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555845.1">
        <property type="nucleotide sequence ID" value="XM_002555799.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNP7"/>
    <dbReference type="FunCoup" id="C5DNP7">
        <property type="interactions" value="1567"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNP7"/>
    <dbReference type="GeneID" id="8294140"/>
    <dbReference type="KEGG" id="lth:KLTH0G18832g"/>
    <dbReference type="eggNOG" id="KOG0676">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027965_0_2_1"/>
    <dbReference type="InParanoid" id="C5DNP7"/>
    <dbReference type="OMA" id="FHTTAER"/>
    <dbReference type="OrthoDB" id="5132116at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10224">
        <property type="entry name" value="ASKHA_NBD_actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.36.70:FF:000001">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000148">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000001">
        <property type="entry name" value="Actin, muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000404">
        <property type="entry name" value="Major actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000058">
        <property type="entry name" value="Putative actin-related protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020902">
        <property type="entry name" value="Actin/actin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004001">
        <property type="entry name" value="Actin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00190">
        <property type="entry name" value="ACTIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00406">
        <property type="entry name" value="ACTINS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00432">
        <property type="entry name" value="ACTINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01132">
        <property type="entry name" value="ACTINS_ACT_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR25408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="375" mass="41604" checksum="F3128B82181057BA" modified="2009-07-28" version="1">MDSEVAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGIMVGMGQKDSYVGDEAQSKRGILTLRYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPMNPKSNREKMTQILFETFNVPAFYVSIQAVLSLYSSGRTTGIVLDSGDGVTHVVPIYAGFSLPHGILRIDLAGRDMTDYLMKILSERGYSFSTTAEREIVRDIKEKLCYVALDFEQEMQTAAQSSAIEKSYELPDGQVITIGNERFRAPEALFHPSLLGLEAAGIDQTAYNSIMKCDVDVRKELYGNIVMSGGTTMFPGIAERMQKEITALAPSSMKVKIIAPPERKYSVWIGGSILASLTTFQQMWISKQEYDESGPSIVHLKCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNR5</accession>
    <name>C5DNR5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0G19228p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0G19228g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555863.1">
        <property type="nucleotide sequence ID" value="XM_002555817.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNR5"/>
    <dbReference type="FunCoup" id="C5DNR5">
        <property type="interactions" value="334"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNR5"/>
    <dbReference type="GeneID" id="8294158"/>
    <dbReference type="KEGG" id="lth:KLTH0G19228g"/>
    <dbReference type="eggNOG" id="ENOG502S3ZS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086083_0_0_1"/>
    <dbReference type="InParanoid" id="C5DNR5"/>
    <dbReference type="OMA" id="GIECEND"/>
    <dbReference type="OrthoDB" id="4060584at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="152"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR25426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="295" mass="32836" checksum="C2F1F61463BD883E" modified="2009-07-28" version="1">MAPATPPRSRNRRGKAVCPSSPRLLTRPAVPVTPGSVSGKRHTGQLQKLRSPLNRSPARGLRTPEYTPARPAGVDASAQARRKLEFPDDQQELAGVGRVLFPGPSSDASTFESRLLSPPAPGRLLRDAPALLPPAPASKAFAAILDEQFFEDDPLQDRECSPTPRGWETPPRATEVPPEQAPSTPSDKIVTFDLAKDWNNNSSRCFSSDEEPDEISIRGKVLENPFADHIVADEETRRRRQELLLAEEPALQDTVSYIDKKGETSKKRHLNAEEQERFRPRMLFEQHFRKKGSQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNR6</accession>
    <name>C5DNR6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0G19250p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0G19250g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555864.1">
        <property type="nucleotide sequence ID" value="XM_002555818.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNR6"/>
    <dbReference type="FunCoup" id="C5DNR6">
        <property type="interactions" value="77"/>
    </dbReference>
    <dbReference type="GeneID" id="8294159"/>
    <dbReference type="KEGG" id="lth:KLTH0G19250g"/>
    <dbReference type="eggNOG" id="ENOG502QQWB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008201_1_0_1"/>
    <dbReference type="InParanoid" id="C5DNR6"/>
    <dbReference type="OMA" id="WSQKQPE"/>
    <dbReference type="OrthoDB" id="2155291at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000935">
        <property type="term" value="C:division septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04399">
        <property type="entry name" value="RhoGAP_fRGD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000591">
        <property type="entry name" value="DEP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065">
        <property type="entry name" value="PROLINE-SERINE-THREONINE PHOSPHATASE INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065:SF17">
        <property type="entry name" value="RHO-GTPASE-ACTIVATING PROTEIN RGD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00610">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00611">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00049">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00055">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50186">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-BAR" evidence="5">
        <location>
            <begin position="2"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="DEP" evidence="3">
        <location>
            <begin position="230"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="4">
        <location>
            <begin position="471"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="694"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="701"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="711"/>
            <end position="723"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR25427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="723" mass="82574" checksum="379321CCDAD181DC" modified="2009-07-28" version="1">MPSFSDSFWTDDLSAGIERLFEELHKGCNQNVLFIQLFASRMQFEVNYGRQLCKTTSSVEGLSATTGSLFSLDIALNHVADAMEEEGRAHISAAADIETTVLRPFSAWCDDHEQRVSYSEKILKTNVSNYQKSKKYVEKLEQAYFNRCRQLEDYKRANYNDEELANAMKQLEIYQEQEHSLAKEREYEKFGKFGNIDFDVRTMRETVRLLLTKLEKSDYKVPFINYHFYNTNNGSEIVKFLMENLTLKDVDQAEAFGQDLLNGGFLKYCNGMGTTFVNSKKFQYSWKPYAYKFAQLSQPNANEVEDENTEESSQAFSDYFTDLTTIITQNQNAGSKNSNLPTVSNTERALFKLIKEMEAADLKYRKECKKIDSLRCSLEELIVDHFTFMEKCELDRLKALEKVVLDFSPIISKNASAVESFQESVLKAASTVDPTLDLMRLVENARTGFFQPRVIPYNNYYNPGGYQNFGIDLETRCRLDKKIVPLIVSSILSYMDQVYPELPNDNVRTTVWTVPVKLHSTHILRAVLNQKPFKDESEVFEKLKEFNEEPSTIASALKIYLLELPEPLISNDVYDTLKALYAEFPPSELEDESQASVDSQRVSGISSAFNALSKPQIATLDAITSHFSRLIKILKMGSSETSEALANEFIRSISEEFANCIIEVKLPDGNDLGYKIFSDLLLYRKPIFRGLKRQNSKIRDPTSSTSSPRSQAHQGDNFGTISH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNR8</accession>
    <name>C5DNR8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0G19294p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0G19294g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555866.1">
        <property type="nucleotide sequence ID" value="XM_002555820.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNR8"/>
    <dbReference type="FunCoup" id="C5DNR8">
        <property type="interactions" value="171"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNR8"/>
    <dbReference type="GeneID" id="8294161"/>
    <dbReference type="KEGG" id="lth:KLTH0G19294g"/>
    <dbReference type="eggNOG" id="ENOG502QR1C">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050572_0_0_1"/>
    <dbReference type="InParanoid" id="C5DNR8"/>
    <dbReference type="OMA" id="VMAYYTF"/>
    <dbReference type="OrthoDB" id="10265193at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005537">
        <property type="term" value="F:D-mannose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06903">
        <property type="entry name" value="lectin_EMP46_EMP47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035661">
        <property type="entry name" value="EMP46/EMP47_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051136">
        <property type="entry name" value="Intracellular_Lectin-GPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005052">
        <property type="entry name" value="Lectin_leg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12223:SF28">
        <property type="entry name" value="LECTIN, MANNOSE BINDING 1 LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12223">
        <property type="entry name" value="VESICULAR MANNOSE-BINDING LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03388">
        <property type="entry name" value="Lectin_leg-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51328">
        <property type="entry name" value="L_LECTIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950564" evidence="7">
        <location>
            <begin position="19"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="396"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="domain" description="L-type lectin-like" evidence="8">
        <location>
            <begin position="25"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR25429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="427" mass="47792" checksum="AB6BED2BC4D2EA77" modified="2009-07-28" version="1">MLVQHLWTLAAFAQLGLAHSKPKTEEQLLNNDFSLPDLIQTKKLPDAWVPGENAVLEEGRVVLTPKKSSKGSLWTKNAYTLGTSFTAEWTVRSTNHRGKSPGGLALWFVNANSPQDTELFNGPSKFEGLQLLIDNNGELESTLRGQLNDGTQQFTSANIYDKTFGSCLLAYQDSTVPLTVRLTYDSANEHFFKVQVDNRVCFQTKKIHFPAENYKFGITATNADNEESFELLQLEVFDGVLEEALKPNANAMEQPKLFTKLVNEKTGDEELRETSPLEMAAAGTAITNNDLLKKMNRLEGKILANDIGILASTIDELIQVQKQQAKNLEKVLAMLGSKSIGQESTDSTGDESFKDFFKLDEKLEKLLTEQRKLREASKTHTSSNGGPHIDEVVRKLTIWLIPLVAVMMVMAYYTFRIRQEIVKTKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2B7</accession>
    <name>C5E2B7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosomal protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0H03652g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556040.1">
        <property type="nucleotide sequence ID" value="XM_002555994.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2B7"/>
    <dbReference type="FunCoup" id="C5E2B7">
        <property type="interactions" value="1179"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2B7"/>
    <dbReference type="GeneID" id="8294353"/>
    <dbReference type="KEGG" id="lth:KLTH0H03652g"/>
    <dbReference type="eggNOG" id="KOG1570">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062853_3_0_1"/>
    <dbReference type="InParanoid" id="C5E2B7"/>
    <dbReference type="OMA" id="GPRNKMP"/>
    <dbReference type="OrthoDB" id="2449818at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00403">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.190.20:FF:000006">
        <property type="entry name" value="Ribosomal protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.790:FF:000002">
        <property type="entry name" value="Ribosomal protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.190.20:FF:000009">
        <property type="entry name" value="Ribosomal protein L10a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050257">
        <property type="entry name" value="eL8/uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002143">
        <property type="entry name" value="Ribosomal_uL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023674">
        <property type="entry name" value="Ribosomal_uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028364">
        <property type="entry name" value="Ribosomal_uL1/biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016095">
        <property type="entry name" value="Ribosomal_uL1_3-a/b-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023673">
        <property type="entry name" value="Ribosomal_uL1_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23105">
        <property type="entry name" value="RIBOSOMAL PROTEIN L7AE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00687">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002155">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56808">
        <property type="entry name" value="Ribosomal protein L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01199">
        <property type="entry name" value="RIBOSOMAL_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR30178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="217" mass="24392" checksum="240EA6B395ABB70E" modified="2009-07-28" version="1">MSKITSTQIRDNVKEVLKYSNETKKRNFLETVELQVGLKNYDPQRDKRFSGALKLPQCPRPNLSICIFGDAFDVDRAKSCGVDAMSVDDLKKLNKNKKLIKKLAKKYSAFVASEVLIKQVPRLLGPQLSKAGKFPTPVSHNEDLYAKVTDVKSTIKFQLKKVLCLAVAVGNIEMDEDTLVNQILMAVNFLVSLLKKNWQNVGSLVIKSTMGPPFRLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2H5</accession>
    <name>C5E2H5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0H04994p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0H04994g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556098.1">
        <property type="nucleotide sequence ID" value="XM_002556052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2H5"/>
    <dbReference type="FunCoup" id="C5E2H5">
        <property type="interactions" value="104"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2H5"/>
    <dbReference type="GeneID" id="8294412"/>
    <dbReference type="KEGG" id="lth:KLTH0H04994g"/>
    <dbReference type="eggNOG" id="ENOG502S20P">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012537_0_0_1"/>
    <dbReference type="InParanoid" id="C5E2H5"/>
    <dbReference type="OMA" id="HSFDFVP"/>
    <dbReference type="OrthoDB" id="5592879at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990758">
        <property type="term" value="P:mitotic sister chromatid biorientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034501">
        <property type="term" value="P:protein localization to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033338">
        <property type="entry name" value="Spc105/Spc7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013253">
        <property type="entry name" value="Spc7_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28260">
        <property type="entry name" value="SPINDLE POLE BODY COMPONENT SPC105"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28260:SF1">
        <property type="entry name" value="SPINDLE POLE BODY COMPONENT SPC105"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08317">
        <property type="entry name" value="Spc7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00787">
        <property type="entry name" value="Spc7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Spc7 kinetochore protein" evidence="3">
        <location>
            <begin position="440"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="118"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="200"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="258"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="645"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="79"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="118"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="142"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="169"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR30236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="904" mass="102306" checksum="AD8767FCAAE2E457" modified="2009-07-28" version="1">MVVVYTSWATSFMNQEFGKTSKTSLDGKAQVKERSKSMVPGRSILKQKFLFNDGGSENLTLSNIPIFSSSQEHEDADGRNNTTSRLNTSKLQSKLDRRVSFAPDVTLHRVDFVPQDRSTFREPRRKPAAEAFTDDFSKEHVQNSQERTYNEESMQLTNNYGGPLVDNSMILNSEQGNQSTPYTPAYDKEVSMEITQLFSKHSAGPENNSEEIIGSRQSSAHQEEEDMEVTQLHKEGIQHEEEGESMVMTEVFQPPQKESYISQPDRSRNSPKNNGEETSDMETTEIVRANQAGMSKPNDASLTDEVSHATSLTVPMQAERDVFDIRSSQFVTSTQKDREEQNFGAQKDRNIETSGRVFDENISRKKRRVSSAHGFDEDGASAAKIPHGAETDEDMEITMMERLSPIAISINMSNSPKTSIPKENINASSAYDRNFPDVTVDKTREQASLKAFLHSVGISSLLSDTELELLPINRMSFVSSQKGRKWPVVEVYYGLYANMPILEIYAFCCKELLRRILKSRELFKELEEEIARNPAPALFKRYFESGTLEREKLSDQITLLRRFSRLEAEKVWLEWRSRHLNGIKNVLQENLLLLKDEYNSIMDHVDKVNRAKRNILNTRQNILTEIRLAQSLTQSKSPSPGIPDRLKLEALKAELVQEMQALKSSSEFEKRMKAVRSDIKNVKLEIERTRSAINSLRSEVKGSPAFAAHQAENLRATFDIVQKICGISFKSYRKSLLTVQLNDPPITATFDIKRAQQVGDVDLAFPNSLKGIAKEIYARSVRTASSCESNVYQFIRALSHDSFNAHLLLTEYKRLKLLLPCKVVEVSGSIDIEITCFDSKKKKFLLLELSLNEFVSAVKGLASSGPKLRVRGLCSERLQPEDALKFLQTKASGVLPWLKEPTLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2K4</accession>
    <name>C5E2K4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0H05698p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0H05698g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556127.1">
        <property type="nucleotide sequence ID" value="XM_002556081.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2K4"/>
    <dbReference type="FunCoup" id="C5E2K4">
        <property type="interactions" value="728"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2K4"/>
    <dbReference type="GeneID" id="8294442"/>
    <dbReference type="KEGG" id="lth:KLTH0H05698g"/>
    <dbReference type="eggNOG" id="KOG0569">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_30_5_1"/>
    <dbReference type="InParanoid" id="C5E2K4"/>
    <dbReference type="OMA" id="ATHACEH"/>
    <dbReference type="OrthoDB" id="4540492at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015149">
        <property type="term" value="F:hexose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045263">
        <property type="entry name" value="GLUT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503:SF8">
        <property type="entry name" value="FACILITATED GLUCOSE TRANSPORTER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="14"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="82"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="112"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="137"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="170"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="198"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="315"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="347"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="378"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="410"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="443"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="468"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="21"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="253"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="260"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="281"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR30265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="503" mass="54682" checksum="A250B5DB4C464CA9" modified="2009-07-28" version="1">MSENQALLGPKPKVTSSLVFATIVVCFGSIQYGYHMAELNAPGQVLSCTEFIEPWPEAGPYADTWFGKHGFQQCLPLTEEQMGFATSIFSIGGLVGSLYAGRVADRYGRKKYSFTNCVIGIIGSLLLFRANSYAQLIAGRFIAGISCGSCIVVTPLFINEISPKELRGSLGSMNQVCINVGILLTQLLALKYADSYRWRWLMLAGAILALIQFLLLFKIDESPLWLASRGDLDGAEHVLRGLRGGDRRRSRGEVDEWLQSRGSSRPATGSADYTQMNSHASQQSASPESVTSSSSQQDVSVKNYFTDPRYSKSRWAITVILVSQQFCGIGSIIFYGVKLVSGQLPQYAVLVNFGISILNVVVTLGASSLVDHWGRKPLLTASAGAISISSIFLSTGIVTNNAAMLVASTFMYIAVFAIGLGPIPFLVISELSPPEASGIAQSYGTTCNWIGTFIVGYGFPILNEWLHGYSFLVFAAYAAAFTLYIHLKVPETKGKQDYDLVWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2Y7</accession>
    <name>C5E2Y7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0H08888p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0H08888g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556260.1">
        <property type="nucleotide sequence ID" value="XM_002556214.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2Y7"/>
    <dbReference type="STRING" id="559295.C5E2Y7"/>
    <dbReference type="GeneID" id="8294585"/>
    <dbReference type="KEGG" id="lth:KLTH0H08888g"/>
    <dbReference type="eggNOG" id="ENOG502QQD4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017959_7_0_1"/>
    <dbReference type="InParanoid" id="C5E2Y7"/>
    <dbReference type="OMA" id="MVVSMTE"/>
    <dbReference type="OrthoDB" id="1641903at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042907">
        <property type="term" value="F:xanthine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006043">
        <property type="entry name" value="NCS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006042">
        <property type="entry name" value="Xan_ur_permease"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00801">
        <property type="entry name" value="ncs2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42810">
        <property type="entry name" value="PURINE PERMEASE C1399.01C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42810:SF2">
        <property type="entry name" value="PURINE PERMEASE C1399.01C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00860">
        <property type="entry name" value="Xan_ur_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="80"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="115"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="190"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="268"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="299"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="338"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="425"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="453"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="484"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="523"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="586"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="593"/>
            <end position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR30398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="620" mass="66642" checksum="DB1125726F0305B4" modified="2009-07-28" version="1">MEFEESSQEASAVRVGSRVRSTWQKILHKFTTREGLLGDYDYAFLFTPSYPFKDWVNRKRGIEPMHRDQPFFSVHSEMPILLGLLLGLQHSLAMLAGVITPPIIISSVANFPMEVQQYLVSASLIVSAIMSSIQITRFHIPYTPYYIGSGLLSVVGTSFATITIVEKAIPVMYTSGFCPMEADGTKGACYAGYGALLGTAACCALLEVAMSFTPPKVLQKVFPKIVTGPVVLCIAVSLIQSGFNDWVGGSGCVGAICPSEGAPMAGEWGSARFIGLGFLVFTTIVTCEKWGSPIMKSCAVIVGLIVGCIVAAACGYFDHSSIDSAPAATFLWVHTFPLKVYGPIVLPMLVMYIVLAQECIGDVTATCDVSRMEVEGEIYETRIQGAVLSDGLSGILSALFTLPPMSTFAQNNGVISLTKCANRQVGYWCCFFLLLMGIFAKFGAALVSIPKPVLGGMTTFLFTSVAVSGLKIISTIPFTRRDRFVLTGTLLFGFGAILVPNWFETFFHYSGSNQSLQGFLDAIILIMETGFAIAGVIGIILNLMIPQELDEDLMDDIEIQQGNLTALEGQRSALAEGGPVLTGKAKEAMSKDNGVSNPQNSFEGKDDRIFVSAGETSPST</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>C5E300</accession>
    <name>C5E300_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0H09174p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0H09174g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556273.1">
        <property type="nucleotide sequence ID" value="XM_002556227.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E300"/>
    <dbReference type="GeneID" id="8294598"/>
    <dbReference type="KEGG" id="lth:KLTH0H09174g"/>
    <dbReference type="eggNOG" id="ENOG502SFJI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125577_1_0_1"/>
    <dbReference type="InParanoid" id="C5E300"/>
    <dbReference type="OMA" id="SAFNDFC"/>
    <dbReference type="OrthoDB" id="2563506at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024368">
        <property type="entry name" value="Ecl1/2/3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12855">
        <property type="entry name" value="Ecl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR30411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="122" mass="13486" checksum="04364F7F4689D798" modified="2009-07-28" version="1">MSAFNDFCIVCERLIDGSSRLYCSQACCERDTSAGRRKSVVESLVATPQLCPLDMDAEDSDGDEDFFELDYTSTVPTTLSSPRAFAKEQQGGYFSLARDVVFDHTAENNYKLWLNAHGAHAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5E358</accession>
    <name>C5E358_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0H10582p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0H10582g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556331.1">
        <property type="nucleotide sequence ID" value="XM_002556285.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E358"/>
    <dbReference type="FunCoup" id="C5E358">
        <property type="interactions" value="261"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E358"/>
    <dbReference type="GeneID" id="8294662"/>
    <dbReference type="KEGG" id="lth:KLTH0H10582g"/>
    <dbReference type="eggNOG" id="KOG0032">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010228_2_2_1"/>
    <dbReference type="InParanoid" id="C5E358"/>
    <dbReference type="OMA" id="MICYFIV"/>
    <dbReference type="OrthoDB" id="1405469at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050339">
        <property type="entry name" value="CC_SR_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042:SF138">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE IKS1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="133"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR30469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="581" mass="63844" checksum="FD6CBFF2E22C4536" modified="2009-07-28" version="1">MSLIVYGDKIDDENNESVVLQDPSSRSLVVMNQESGEISLLRQIRGPQNGSRRKGGTGLISSYFCPQCGSEIGCGYNFASSPPSTKKPSGSFVHKNYFNLLEHRSSQTQLPSIESPSAIPASLFTPGYYQRFFKELSLLGSGARGSVFKVEHVLMDNHLGVYALKKIHIGNNLSWLERGMKEVKFLSALTHSSVNLITYNHVWLEMDNASGIVKARNGEEVGPPESVPCVFILQQFCCGGNLEEVVLNKVFEKFTDHESPEARKRRFRLRRSANAHGSRQLGLGTNQILSIIHDVASGLKELHDLNIIHRDLKPSNCLLLESYEAGKEGYGDKSFPTVVISDFGESQLGGQPRSATGATGTLEFTAPEVVITNSENLSANLPQFSFQSDMYSLGMVCYFLVFGELPFGCQQSLPELKKNIKLLSITKEGLSQKHDDMKLHPIDSAIFVLIEKLLSRDALSRPTAGEVKAEIAEIMDRLDDCVSATFDEKQELLSDDESELQSIFGRQPEEISAKSGPSCSAIIDLANVATVLIAICFFNEKSLIPCVCVALLALSLKVPPPARKLFLITELTLLIVAHLAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3C5</accession>
    <name>C5E3C5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cysteine desulfurase, mitochondrial</fullName>
            <ecNumber evidence="3">2.8.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">tRNA-splicing protein SPL1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0H12210g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the removal of elemental sulfur from cysteine to produce alanine. It supplies the inorganic sulfur for iron-sulfur (Fe-S) clusters. Plays a role in both tRNA-processing and mitochondrial metabolism. Involved in the 2-thio-modification of both 5-carboxymethylaminomethyl-2-thiouridine in mitochondrial tRNAs and 5-methoxycarbonylmethyl-2-thiouridine (mcm5s2U) in cytoplasmic tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(sulfur carrier)-H + L-cysteine = (sulfur carrier)-SH + L-alanine</text>
            <dbReference type="Rhea" id="RHEA:43892"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="EC" id="2.8.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.7" evidence="3"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556398.1">
        <property type="nucleotide sequence ID" value="XM_002556352.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3C5"/>
    <dbReference type="FunCoup" id="C5E3C5">
        <property type="interactions" value="947"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3C5"/>
    <dbReference type="GeneID" id="8294735"/>
    <dbReference type="KEGG" id="lth:KLTH0H12210g"/>
    <dbReference type="eggNOG" id="KOG1549">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003433_0_2_1"/>
    <dbReference type="InParanoid" id="C5E3C5"/>
    <dbReference type="OMA" id="KGLYWAR"/>
    <dbReference type="OrthoDB" id="10250117at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990221">
        <property type="term" value="C:L-cysteine desulfurase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031071">
        <property type="term" value="F:cysteine desulfurase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044571">
        <property type="term" value="P:[2Fe-2S] cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034227">
        <property type="term" value="P:tRNA thio-modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000003">
        <property type="entry name" value="Cysteine desulfurase IscS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000002">
        <property type="entry name" value="Cysteine desulfurase IscS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00331">
        <property type="entry name" value="Cys_desulf_IscS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020578">
        <property type="entry name" value="Aminotrans_V_PyrdxlP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010240">
        <property type="entry name" value="Cys_deSase_IscS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002806">
        <property type="entry name" value="PRK02948.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010611">
        <property type="entry name" value="PRK14012.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11601:SF34">
        <property type="entry name" value="CYSTEINE DESULFURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11601">
        <property type="entry name" value="CYSTEINE DESULFURYLASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00595">
        <property type="entry name" value="AA_TRANSFER_CLASS_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="14">
        <location>
            <begin position="81"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00045623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00050776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00080923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR30536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="478" mass="52807" checksum="63EFCD9AFFD80219" modified="2009-07-28" version="1">MLRSFGRSVFSRQRLVGAVARRGYATAKVAFDVPLETHTDLQKANEDLAQARSNSAEVIESGSTALKHAYQENTGFGTRPIYLDMQATTPTDPRVLDTMLKFYTGLYGNPHSNTHSYGWETNKEVETARKHIADVINADVKEIVFTSGATESNNMALKGVSRFYKKTKNHIITTRTEHKCVLEAARAMKNEGYDVTFLNVDEYGLVDLKELEAAIKPTTCLVSVMAVNNEIGVMQPIKEIGAICRKNKVFFHTDAAQAYGKIPLDVNDMNIDLLSISSHKIYGPKGMGALFVRRRPRVRLDPIISGGGQERGLRSGTLAPSLVAGFGEAARLVKQEYETDIDHVKRLSEKLTKGLLAIEHTSLNGSPDHRYPGCVNVSFAYVEGESLLMALRDIALSSGSACTSASLEPSYVLHALGKDDAMAHSSIRFGIGRFTTDEEIDYVIKAITERVEFLRELSPLWEMVKEGIDLNSIEWSGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3H5</accession>
    <name>C5E3H5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0H13618p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0H13618g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556448.1">
        <property type="nucleotide sequence ID" value="XM_002556402.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3H5"/>
    <dbReference type="FunCoup" id="C5E3H5">
        <property type="interactions" value="39"/>
    </dbReference>
    <dbReference type="GeneID" id="8294809"/>
    <dbReference type="KEGG" id="lth:KLTH0H13618g"/>
    <dbReference type="eggNOG" id="ENOG502S2GH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026649_0_0_1"/>
    <dbReference type="InParanoid" id="C5E3H5"/>
    <dbReference type="OMA" id="WPNIRIR"/>
    <dbReference type="OrthoDB" id="4034650at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013932">
        <property type="entry name" value="TATA-bd_TIP120"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08623">
        <property type="entry name" value="TIP120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TATA-binding protein interacting (TIP20)" evidence="1">
        <location>
            <begin position="493"/>
            <end position="621"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR30586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="660" mass="74489" checksum="9288D7AE9E5EE507" modified="2009-07-28" version="1">MLLSDIIDQYFSTQDHDLRYMALREHILNARDRWSDVQLQGLVSRVLIPALSDRDTAIVELVSTQVFPHVAALHPLETEPCIILPLCQRLRFPPENLELARQEVSPNEPQDRPQVLQSLRNVLSTFKSPLVSPQPLDIYCNCFQRMLERPYLAWETLGLLIQLSEDLHVIETWYARLYMLALQDGRLTAMNAMQIATGRVSPNVILQCLKQAGPFTEAHARLLSQITQEHAAFRLCYGELVQLLLQQPLTETVCLTLANLSVWLQQPAIEAASPMKSTLADALFGRCCTILSSASEMNQPPLEDEDPTQDSYLKQLSDVSGDEDNEYEFEDEEDDEAALAPKLCLQLLRLLPLQIPQYVIDRLESPNLNEDALLELIGEKAINLPHVVSRLPKAADRLPIDKMPLNVLEVLPSLPSVQKRIAYFTESSILPSNAPLILANTIIKDAEHINENTANCLVEVLNHHLNLEVADLEQYQLAIDAAASAAKRSEVHHLHDHIAQMVWPHLKPNKAFIRTIKVGNMKQAIDDGVSFRLNCYTLLQQLDLSYTAQCVILQECAERGFKDESSIKEAAAELFTKVLELAWPNIRIRDATWFVEHLCPRVQDRVQKLLSAEPQATATQHQIADWARGLKVLEHVELVTGDIFNIVKNDLDCTFMGPTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3T4</accession>
    <name>C5E3T4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0H16148p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0H16148g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556557.1">
        <property type="nucleotide sequence ID" value="XM_002556511.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3T4"/>
    <dbReference type="STRING" id="559295.C5E3T4"/>
    <dbReference type="GeneID" id="8294923"/>
    <dbReference type="KEGG" id="lth:KLTH0H16148g"/>
    <dbReference type="eggNOG" id="KOG0538">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020639_0_1_1"/>
    <dbReference type="InParanoid" id="C5E3T4"/>
    <dbReference type="OMA" id="WFQLYWL"/>
    <dbReference type="OrthoDB" id="1925334at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03332">
        <property type="entry name" value="LMO_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000132">
        <property type="entry name" value="FMN dependent dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012133">
        <property type="entry name" value="Alpha-hydoxy_acid_DH_FMN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000262">
        <property type="entry name" value="FMN-dep_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037396">
        <property type="entry name" value="FMN_HAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008259">
        <property type="entry name" value="FMN_hydac_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037350">
        <property type="entry name" value="LMO_FMN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578:SF143">
        <property type="entry name" value="FMN-DEPENDENT ALPHA-HYDROXY ACID DEHYDROGENASE PB1A11.03"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578">
        <property type="entry name" value="S -2-HYDROXY-ACID OXIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01070">
        <property type="entry name" value="FMN_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000138">
        <property type="entry name" value="Al-hdrx_acd_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00557">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51349">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3 7" id="KW-0288">FMN</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN hydroxy acid dehydrogenase" evidence="8">
        <location>
            <begin position="26"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="300"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="106"/>
            <end position="108"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="276"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="298"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="331"/>
            <end position="335"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="354"/>
            <end position="355"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000138-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000138-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR30695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="406" mass="44706" checksum="F12A199D154D57F4" modified="2009-07-28" version="1">MEYLETSAGNPLQYMADVYGRGLKYERPPFTFQSTEWEDLARERMSRESLGYVCGNASTGETTRKNHEAFQKWSIVPSRLVETSTYPSLKTELLNQKLPVPIAIAPIGVQRIFNPDGELATAAAAASEGVPYIYSTAAATSIEDVAKVNGNGVRWYQLYWPSNDHNDLTVSLLKRAKENGFSTLVVTLDTYKLGWRPSDLNNAYNPFLRSDTVGTALGFSDPVFRKNFKEKYGKEVEEDMKTAASDWAGMIFPGFSHGWEDLKFLRENWDGPIVLKGIQTVLDAKKCVELGIEGIVVSNHGGRQQDGGPASLDRLVKIVNEVGSKIDIIFDSGIRCGSDIAKALALGAKMVLVGRPYVYGLVLGGEEGVGHVLRSLLGDLTMNLHLAGIKSVSPEHLNRDCLEYSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-02-15" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q5K464</accession>
    <name>Q5K464_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Putative DNA endonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">io-cox1.2</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <geneLocation type="mitochondrion" evidence="9"/>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2005" name="FEBS Lett." volume="579" first="30" last="40">
            <title>The complete mitochondrial genome of the yeast Kluyveromyces thermotolerans.</title>
            <authorList>
                <person name="Talla E."/>
                <person name="Anthouard V."/>
                <person name="Bouchier C."/>
                <person name="Frangeul L."/>
                <person name="Dujon B."/>
            </authorList>
            <dbReference type="PubMed" id="15620687"/>
            <dbReference type="DOI" id="10.1016/j.febslet.2004.10.106"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the LAGLIDADG endonuclease family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the heme-copper respiratory oxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="AJ634268">
        <property type="protein sequence ID" value="CAG25600.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_184721.1">
        <property type="nucleotide sequence ID" value="NC_006626.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q5K464"/>
    <dbReference type="FunCoup" id="Q5K464">
        <property type="interactions" value="88"/>
    </dbReference>
    <dbReference type="STRING" id="559295.Q5K464"/>
    <dbReference type="GeneID" id="3238979"/>
    <dbReference type="InParanoid" id="Q5K464"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Mitochondrion"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015990">
        <property type="term" value="P:electron transport coupled proton transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006314">
        <property type="term" value="P:intron homing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.210.10">
        <property type="entry name" value="Cytochrome c oxidase-like, subunit I domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.28.10">
        <property type="entry name" value="Homing endonucleases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023616">
        <property type="entry name" value="Cyt_c_oxase-like_su1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036927">
        <property type="entry name" value="Cyt_c_oxase-like_su1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000883">
        <property type="entry name" value="Cyt_C_Oxase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027434">
        <property type="entry name" value="Homing_endonucl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004860">
        <property type="entry name" value="LAGLIDADG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10422">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10422:SF18">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00115">
        <property type="entry name" value="COX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00961">
        <property type="entry name" value="LAGLIDADG_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01165">
        <property type="entry name" value="CYCOXIDASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81442">
        <property type="entry name" value="Cytochrome c oxidase subunit I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55608">
        <property type="entry name" value="Homing endonucleases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50855">
        <property type="entry name" value="COX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0404">Intron homing</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="57"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="104"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome oxidase subunit I profile" evidence="8">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAG25600.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="542" mass="63045" checksum="E675C6877DB70BCA" modified="2005-02-15" version="1">MVQRWLYSTNAKDIAILYFIFAIFCGMAGTAMSVIIRLELAAPGNQYLGGNHQLFNVLVVGHAVLMIFFLVMPALIGGFGNYLLPLMIGASDMSFARLNNISFWLLPPALVCLVTSTLVESGAGTGWTVIMNEMMCSCKMSTDAWKTLYNFIMYTLKYSIYINKLVKMFNIMGLYASVMFNYLTTQRTNVTKLKYYMKKKYSTTNKTNKSYFNMNEWLVGMTDGDGTFNIYTNMTHNKIIFTYKISLNKKNTQLLYKIKSYLGVGSVTFDNKTNMASYLIRDKKYLLNIIIPIFDEYPLLTTKRYNYLKFKECLLISNNNLLTQNDKLLTINNIKNKTSSLDLYSDSLKKYNIYISDAWESIYKKWNMKEDLTYTPMNTNDGVLIYPHKDIINNINSITINDVNNIMTKSWLIGFIEAKGSFFITKKSSNPLRCVHSFELTQKLDYIVMYSIKLLLNINSSIVMKSPSISNKSNLFYKLETTNNSNIEYIINYFRYKNYKSVFLGMKSFEYRIWSRTYMKHKGNYMKLLQIKNLLQKHRKNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DB91</accession>
    <name>C5DB91_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0A00638p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0A00638g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551490.1">
        <property type="nucleotide sequence ID" value="XM_002551444.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DB91">
        <property type="interactions" value="561"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DB91"/>
    <dbReference type="GeneID" id="8290281"/>
    <dbReference type="KEGG" id="lth:KLTH0A00638g"/>
    <dbReference type="eggNOG" id="KOG1127">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001688_0_0_1"/>
    <dbReference type="InParanoid" id="C5DB91"/>
    <dbReference type="OMA" id="CQWELDP"/>
    <dbReference type="OrthoDB" id="421075at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055087">
        <property type="term" value="C:Ski complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039226">
        <property type="entry name" value="Ski3/TTC37"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040962">
        <property type="entry name" value="TPR_22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15704">
        <property type="entry name" value="SUPERKILLER 3 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15704:SF7">
        <property type="entry name" value="SUPERKILLER COMPLEX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18833">
        <property type="entry name" value="TPR_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="38"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="681"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="715"/>
            <end position="748"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR21048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1411" mass="160223" checksum="C55D958707082ED4" modified="2009-07-28" version="1">MAELKQLLKDAKNALASEDYEQSIEKSKEVLNLDKNNYFAHVFLGKAFSCTGDLAQSKKHYKAAIDMDPGNMLAWKGIFMLFRTANIAPTIVSFDEFFDLCGEYAEHLVQQQLPMVDLVNDIRNFKNFYRDCQESYLRHMRPGTPMAERLSRHLITPQDALQGLLNIVSAEEQQQVSKLVSRERLKISANQPDYNLKVNSIAWQVYQDSEIDELYQQLINITDDDEKRRGLESKWLEYRIRVLKSMPADIKHHFFTKVRNMVEDMVVVDHDFLLAWQLYFEWQDYSDLDCMDVNVISKFLKKFPKEPLAAVLYAWVCSEQSSYNSKEFYEKTFQGEDTDNSGTSSVDSINDVVEDGMDESEKRRLQQLVDPSMEEATFGLSEDEVLLSLTENIKKTQQSIVAHRIVSHYYVSTREYESALHYTKAGIVLCAQSVKNMGAHLSNSKRELTLDLATTYTYFEAPKNHPTALALFEKLLTENSDNVHAKMGKGLIAIERRNWQEAYELLKEVTERYPDNYEVLSEFAWSELHLDAPDAAIERFKYILNNVEGSDLKVLEFRSLNHWRMAKGYVYKQHGEINAGESSEKEYIKLAFKQLVQAVKTSENFAPGYSTLGDIYSEYYQDPTRAFKCYFKSFEMDPSDLTAARYMCEKYCGSCDWQSASLIAERVVKAEKSKSILQNVNWPYRVLGIAHLEKQAESESIEWFQSALRVEPADLESWLGLGQAYYSCGRVEASVKVFEKALEVSPGNAYAHYFKALSLSKMGLYDESIEIFRELTAEHPDEESYQVSMVEVLVEHCNVLCSQGFLTRSVAAAADAIRKIESIVLHIDYKIHGMWLSLSNALRVICLVQSQVHLLPLESLVNIFEVAELRNTDEIDKIDHVTLSGLLSDLEQDNVAIAAQFLVLSGKHAIACSDYASLPRTVRASLWYNLGIVELMAFVILKSNSHRDAAIESFKKSIEYQSNTAEAWIGFGIASIDLNVRVAQHCFIKATALNPRETSVWYDMAILALRYNDVAFARAVLMKSQSIAPQDSTPWFGLALASEKEGLTQDSARLFAHSFVLSNGKSKGAQLLYAKSVLKTRIGSGDDERDIGPGQELSAVAYGLDDYLKKNPDDKFALQCAILTLERLRNYAAARKLSDRLLDILEKRFEKAQDDSELFHFAIVKSQLARVYLGLKDYETAIESADLSLGILAEHNNEVAEKCITSNHAVMGLASFFLDDFDQTLEHFKVLLDRPGESKQLVILVAKILYNVESEETREVALKELVDYINSNGPDLTVTLTIAVISLLEGIPDDMRAILSELKSLPLSDMISDRHRDIPFLIQQLNTKLSQDCESQQQWQRTAFFFPNDSGSWGSLDKKIRARIAAGGQNKVPARKMSEFYSSLGNLSKIQRSIFLCPWNKEALSSLKGCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBE8</accession>
    <name>C5DBE8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0A02024p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0A02024g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M24B family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551547.1">
        <property type="nucleotide sequence ID" value="XM_002551501.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBE8"/>
    <dbReference type="FunCoup" id="C5DBE8">
        <property type="interactions" value="431"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBE8"/>
    <dbReference type="MEROPS" id="M24.A09"/>
    <dbReference type="GeneID" id="8290343"/>
    <dbReference type="KEGG" id="lth:KLTH0A02024g"/>
    <dbReference type="eggNOG" id="KOG2737">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017266_1_2_1"/>
    <dbReference type="InParanoid" id="C5DBE8"/>
    <dbReference type="OMA" id="DAHALFF"/>
    <dbReference type="OrthoDB" id="10261878at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01087">
        <property type="entry name" value="Prolidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.230.10:FF:000002">
        <property type="entry name" value="Xaa-Pro aminopeptidase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007865">
        <property type="entry name" value="Aminopep_P_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052433">
        <property type="entry name" value="X-Pro_dipept-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43226">
        <property type="entry name" value="XAA-PRO AMINOPEPTIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43226:SF1">
        <property type="entry name" value="XAA-PRO DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05195">
        <property type="entry name" value="AMP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01011">
        <property type="entry name" value="AMP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53092">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminopeptidase P N-terminal" evidence="6">
        <location>
            <begin position="48"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR21105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="510" mass="58278" checksum="106E390C5BDFCC11" modified="2009-07-28" version="1">MPFADQLLTFSLAFLAYQLGRDSLEFTFSSRPQQKMSPSDSDLPKGKYPAKKHALKIKKNFLSKKGEEQTESTLFVAGGEIEPIKYCDQTKEFRQNRYFFYLTGVNIPGSAAFFDFKSEKLTLFLPDVDSDDVMWSGLPLSVEAAYEKFDVDEVLYAKKLPSFLSKFESSCIYTTDLDNVHDPNVAEMLVPSDPDLFYALDESRLTKDEYEIGLLRRAAKITDNCHLSVMSALPIEKNEGHFHAEFTYHAIRQGSKHQGYDPICCSGPNCSTLHYVKNDESLENKQSVLMDAGAEWENYTADVTRCFPLNGKFTKEHREIYDTVLKMQTEVMDRIKPGVEWEKLHILAHRVLIRSFLNLGIFKSGYSEEEILDRKASLCFFPHGLGHLLGMDTHDVGGRANYEDSNPLLKFLRLRRPLEENMVVTNEPGVYFNPFLIEEFLIKYPKRKEVVNEEIMEKYMYVGGVRIEDDVLVTADGCENLTGITSDPEEIERIVCDGINKGRSHFHVIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBF6</accession>
    <name>C5DBF6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0A02222p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0A02222g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tRNA pseudouridine synthase TruA family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551555.1">
        <property type="nucleotide sequence ID" value="XM_002551509.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBF6"/>
    <dbReference type="FunCoup" id="C5DBF6">
        <property type="interactions" value="757"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBF6"/>
    <dbReference type="GeneID" id="8290352"/>
    <dbReference type="KEGG" id="lth:KLTH0A02222g"/>
    <dbReference type="eggNOG" id="KOG2554">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014673_2_0_1"/>
    <dbReference type="InParanoid" id="C5DBF6"/>
    <dbReference type="OMA" id="DCKFPEM"/>
    <dbReference type="OrthoDB" id="25767at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990481">
        <property type="term" value="P:mRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02569">
        <property type="entry name" value="PseudoU_synth_ScPus3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.580:FF:000020">
        <property type="entry name" value="tRNA pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.660:FF:000012">
        <property type="entry name" value="tRNA pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.660">
        <property type="entry name" value="Pseudouridine synthase I, catalytic domain, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.580">
        <property type="entry name" value="Pseudouridine synthase I, catalytic domain, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00171">
        <property type="entry name" value="TruA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001406">
        <property type="entry name" value="PsdUridine_synth_TruA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020097">
        <property type="entry name" value="PsdUridine_synth_TruA_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020095">
        <property type="entry name" value="PsdUridine_synth_TruA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041707">
        <property type="entry name" value="Pus3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020094">
        <property type="entry name" value="TruA/RsuA/RluB/E/F_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00071">
        <property type="entry name" value="hisT_truA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142:SF5">
        <property type="entry name" value="TRNA PSEUDOURIDINE(38_39) SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01416">
        <property type="entry name" value="PseudoU_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Pseudouridine synthase I TruA alpha/beta" evidence="7">
        <location>
            <begin position="234"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="41"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="41"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR21113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="443" mass="51593" checksum="9D41A71A1FDA5ABF" modified="2009-07-28" version="1">MSLISKLLRFGRNASKSLPEQCEYAKWSKEDLVRKVMELEAENKRKAEDEGDRSESKTKKAKRDDLPEKRKRKKKDKPFDFSKYDTRFVAFKFAYLGWNYNGLAIQKEPTPLPTVEGTILEAMNACKLVPSMVPQDYNFSRCGRTDKGVSAMNQVISLHVRSNLTREEQQDEENDGREIDYIHILNQLLPDDIRISAVCLRPPTNFDARFSCQYRHYRYLFHKDGLDIKKMEEAARMYEGEHDFRNFCKLDGSKQINNYKRNIERSRIISLSEDIYCLDLIGSAFLWHQVRCMMANLFLIGQGLEDTTLLKDLMDVARVKQKPIYDMASDIPLILFDCKFPEMEWKTVDSNNFKSLQSDRRVYALSLDYNLKSTVANIFRETLCVTTSEIQGRTRINLGDGKGKVVAVYQKMLERGVMESVESVNQKFREKKAAKKRNRMTTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>C5DC39</accession>
    <name>C5DC39_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0A07568p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0A07568g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551788.1">
        <property type="nucleotide sequence ID" value="XM_002551742.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DC39"/>
    <dbReference type="FunCoup" id="C5DC39">
        <property type="interactions" value="29"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DC39"/>
    <dbReference type="GeneID" id="8290595"/>
    <dbReference type="KEGG" id="lth:KLTH0A07568g"/>
    <dbReference type="eggNOG" id="ENOG502S4R3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082503_0_0_1"/>
    <dbReference type="InParanoid" id="C5DC39"/>
    <dbReference type="OMA" id="SHRDPTH"/>
    <dbReference type="OrthoDB" id="4094515at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028095">
        <property type="entry name" value="Mso1_N_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14475">
        <property type="entry name" value="Mso1_Sec1_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mso1 N-terminal" evidence="2">
        <location>
            <begin position="29"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR21346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="183" mass="20535" checksum="889A04238AC5D10A" modified="2009-07-28" version="1">MHQESPAAYQKSGKFWSKVKSSTKSFSSSFSQLSLKQERDGDTPTSTVVHKALVKFYSTQEPFQGFPDWLGHKEDLPDEQKVLRKQKHHSPTHSTKPSEPALHLPSTCSNSAAVTPTPRTAGTDFRGIYKSSSESRATSVDYMGSRPLPRQSQDASRQENTAQLPPPTQRTSSMLMRERLKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCE5</accession>
    <name>C5DCE5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0B02398p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0B02398g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551894.1">
        <property type="nucleotide sequence ID" value="XM_002551848.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCE5"/>
    <dbReference type="FunCoup" id="C5DCE5">
        <property type="interactions" value="47"/>
    </dbReference>
    <dbReference type="GeneID" id="8290724"/>
    <dbReference type="KEGG" id="lth:KLTH0B02398g"/>
    <dbReference type="eggNOG" id="ENOG502S17D">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071646_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCE5"/>
    <dbReference type="OMA" id="CDFFRLL"/>
    <dbReference type="OrthoDB" id="4062049at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR21456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="335" mass="37993" checksum="340240BCDBF26B5E" modified="2009-07-28" version="1">MTSRLVLKELKAGFKATNKFPVTEKTSPVSKATAPKLMGDVMTLGSISATNNFCKPNKRLEKPPRGERALVEVRSMLRGTNSVAQVYQNTFRIFSGQSKVYHCDFFRLLYFPPEHFLSVFRKSKPAVNVRIVGEVFLNGNVSKSQGRSVANTAVYARGSRIEGYGKSQDVSNLKQLQNVFANDGSNVPRNYAYLRRSLRASLNKWFIQHWMALQGPQAVEEQIRRQGEAGSSAQWQYLDAQGRSKTSVAKDGFYLFQVLVFPDKTTKQEFKANVARGVKTVADLEWDDFLRSKSNKSRTWVQECNDRVRVPVMNSYLANDQMPRVLRKLQSSRAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCJ7</accession>
    <name>C5DCJ7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Tyrosine--tRNA ligase</fullName>
            <ecNumber evidence="12">6.1.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Tyrosyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0B03608g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>tRNA(Tyr) + L-tyrosine + ATP = L-tyrosyl-tRNA(Tyr) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10220"/>
            <dbReference type="Rhea" id="RHEA-COMP:9706"/>
            <dbReference type="Rhea" id="RHEA-COMP:9707"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78536"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.1" evidence="12"/>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551946.1">
        <property type="nucleotide sequence ID" value="XM_002551900.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCJ7"/>
    <dbReference type="FunCoup" id="C5DCJ7">
        <property type="interactions" value="841"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCJ7"/>
    <dbReference type="GeneID" id="8290779"/>
    <dbReference type="KEGG" id="lth:KLTH0B03608g"/>
    <dbReference type="eggNOG" id="KOG2144">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035267_0_1_1"/>
    <dbReference type="InParanoid" id="C5DCJ7"/>
    <dbReference type="OMA" id="RKIHMLA"/>
    <dbReference type="OrthoDB" id="197206at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004831">
        <property type="term" value="F:tyrosine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006437">
        <property type="term" value="P:tyrosyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00805">
        <property type="entry name" value="TyrRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.240.10:FF:000004">
        <property type="entry name" value="Tyrosine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000040">
        <property type="entry name" value="Tyrosine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.240.10">
        <property type="entry name" value="Tyrosyl-Transfer RNA Synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002307">
        <property type="entry name" value="Tyr-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023617">
        <property type="entry name" value="Tyr-tRNA-ligase_arc/euk-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050489">
        <property type="entry name" value="Tyr-tRNA_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006330">
        <property type="entry name" value="PRK08560.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00234">
        <property type="entry name" value="tyrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46264:SF4">
        <property type="entry name" value="TYROSINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46264">
        <property type="entry name" value="TYROSINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006588">
        <property type="entry name" value="TyrRS_arch_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01040">
        <property type="entry name" value="TRNASYNTHTYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8 12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="354"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="366"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR21508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="405" mass="45167" checksum="EA7178E5B1ECBDFC" modified="2009-07-28" version="1">MTGESQTIQDPQAAYELITKNLQEVLNPQIIKNVLEVEKRPLKLYWGTAPTGRPHCGYFVPMTKLADFLKAGCEVRVLLADLHAFLDNMKAPLEVVKYRAKYYECVIKSLLRSINVPIEKLDFVVGSSYQLSSEYTMDIFKISNVVSQNDAKRAGADVVKQVANPLLSGLIYPLMQALDEEHLGVDVQFGGVDQRKIFVLAEENLPSLGYKKRAHLMNPMVPGLTQGGKMSASDPNSKIDILEEPKQVKKKINTAFCAPGVVEDNGLLSFIEYVVAPIQELKHGANYFKFYIDRPEKFGGPVTYNSFKELVEDFKNEKLAPPDLKTGVSDIINELLAPIREDFAKDPEFQEAQAKGYPVVSTEKVKKQKKPKNKGTRYPGGQAPNNDPTAVVAEKLEQTELKESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCS4</accession>
    <name>C5DCS4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase</fullName>
            <ecNumber evidence="1">3.1.3.67</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0B05412g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.3.67" evidence="1"/>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552023.1">
        <property type="nucleotide sequence ID" value="XM_002551977.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCS4"/>
    <dbReference type="FunCoup" id="C5DCS4">
        <property type="interactions" value="56"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCS4"/>
    <dbReference type="GeneID" id="8290861"/>
    <dbReference type="KEGG" id="lth:KLTH0B05412g"/>
    <dbReference type="eggNOG" id="KOG2283">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020105_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCS4"/>
    <dbReference type="OMA" id="CWLNLYW"/>
    <dbReference type="OrthoDB" id="16692at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016314">
        <property type="term" value="F:phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043491">
        <property type="term" value="P:phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051896">
        <property type="term" value="P:regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14497">
        <property type="entry name" value="PTP_PTEN-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051281">
        <property type="entry name" value="Dual-spec_lipid-protein_phosph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029023">
        <property type="entry name" value="Tensin_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12305">
        <property type="entry name" value="PHOSPHATASE WITH HOMOLOGY TO TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12305:SF81">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3,4,5-TRISPHOSPHATE 3-PHOSPHATASE AND DUAL-SPECIFICITY PROTEIN PHOSPHATASE PTEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51181">
        <property type="entry name" value="PPASE_TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphatase tensin-type" evidence="5">
        <location>
            <begin position="24"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="4">
        <location>
            <begin position="173"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR21585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="446" mass="51226" checksum="2C3553BA5E545474" modified="2009-07-28" version="1">MPFSLQAFTPENILRSVYSSPIKVHKNDLGFSLDLSYITRELIVCSYPVIKYPQLLYRNSLKDLISYLNLHHGAGNWKIYNFKAEKGASDYSDEDLVTALFELSNFCEKASASAGPLLSTIYDPTKSPCKEGHDSASTQSLSSTRESLLERVGHYIQRYGWLDHSPPPFTLLQEILDDMHEYLIQDSSRVVLLHCKLGKGRSGSVVVAYLMKYMRCSLREGREIFVNNRFKVGLTQGVTISSQLRYLGYHERFLQADHPSNWSKILSELTRAQFMINSFEIIHLLSAAPVKLESKTYTMSIKIQIYNRNRDGLIDVFDTNNKEQSIDSKPHSNLNRVSVGQRITESDIRISFGLRHKGSAFIDNVTQLTSISHFWINLYWETLACSRSESTDHYTLDKLANEQSRCRDRKNFAMFSIKWAELDGMKGLQSKGFKLFETFTLRWQLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCX8</accession>
    <name>C5DCX8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0B06622p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0B06622g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the archaeal Rpo12/eukaryotic RPC10 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552077.1">
        <property type="nucleotide sequence ID" value="XM_002552031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCX8"/>
    <dbReference type="FunCoup" id="C5DCX8">
        <property type="interactions" value="649"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCX8"/>
    <dbReference type="GeneID" id="8290916"/>
    <dbReference type="KEGG" id="lth:KLTH0B06622g"/>
    <dbReference type="eggNOG" id="KOG3507">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_179456_0_1_1"/>
    <dbReference type="InParanoid" id="C5DCX8"/>
    <dbReference type="OMA" id="MTYICGD"/>
    <dbReference type="OrthoDB" id="5585087at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.30:FF:000003">
        <property type="entry name" value="DNA-directed RNA polymerases I, II, and III subunit RPABC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.30">
        <property type="entry name" value="RNA polymerase ii, chain L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006591">
        <property type="entry name" value="RNAP_P/RPABC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039747">
        <property type="entry name" value="RPABC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029040">
        <property type="entry name" value="RPABC4/Spt4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12056">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12056:SF2">
        <property type="entry name" value="GEO11084P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03604">
        <property type="entry name" value="Zn_ribbon_RPAB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00659">
        <property type="entry name" value="RPOLCX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63393">
        <property type="entry name" value="RNA polymerase subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR21639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="70" mass="7687" checksum="06665ECFE9D8D7AE" modified="2009-07-28" version="1">MSREGFQIPTNLDAAAAGTAAARTATLKYICAECSSKLSLSRTDPVRCKDCGHRILLKARTKRMVQFEAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>C5DD05</accession>
    <name>C5DD05_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Importin subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0B07282g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the importin alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552104.1">
        <property type="nucleotide sequence ID" value="XM_002552058.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DD05"/>
    <dbReference type="FunCoup" id="C5DD05">
        <property type="interactions" value="1302"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DD05"/>
    <dbReference type="GeneID" id="8290944"/>
    <dbReference type="KEGG" id="lth:KLTH0B07282g"/>
    <dbReference type="eggNOG" id="KOG0166">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018084_6_0_1"/>
    <dbReference type="InParanoid" id="C5DD05"/>
    <dbReference type="OMA" id="EMIQMLY"/>
    <dbReference type="OrthoDB" id="29145at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061608">
        <property type="term" value="F:nuclear import signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.690:FF:000003">
        <property type="entry name" value="Importin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000021">
        <property type="entry name" value="Importin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.690">
        <property type="entry name" value="Importin-alpha, importin-beta-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032413">
        <property type="entry name" value="Arm_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002652">
        <property type="entry name" value="Importin-a_IBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036975">
        <property type="entry name" value="Importin-a_IBB_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024931">
        <property type="entry name" value="Importin_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23316">
        <property type="entry name" value="IMPORTIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16186">
        <property type="entry name" value="Arm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01749">
        <property type="entry name" value="IBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005673">
        <property type="entry name" value="Importin_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51214">
        <property type="entry name" value="IBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="IBB" evidence="9">
        <location>
            <begin position="35"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="6">
        <location>
            <begin position="164"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="6">
        <location>
            <begin position="207"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="87"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="35"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR005673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR21666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="576" mass="63818" checksum="DF57975F67EB0667" modified="2009-07-28" version="1">MMEPNPSGTVRLQQLSASYKRTRSLSETLRPATQAMDSDSSSTNKFVPEYRRTNFKNKGRFSADELRRRRDTQQVELRKAKRDEALAKRRNFAAPPDGADSEDEDEAASTADQQFYQQLQQELPQMIQQINSTDLQEQLNATVKFRQILSREHRPPIDTVIQSGVVPNLVNFMNENQPEMLQLEAAWALTNIASGSSDQTKVVVEAGAVPLFIQLLYSGSIEVKEQAIWALGNVAGDSTGYRDYVLSCGAMQPILALFDLSKTSLIRTATWTLSNLCRGKKPQPDWGVVSQSLPTLAKLIYSMDTETLVDACWAISYLSDGPANNIQAVIDARIPKRLVELLNHQSTLVQTPALRAVGNIVTGNDLQTQVVINCGVLGALRNLLSSTKESIRKEACWTISNITAGNTDQIQAAIDANLIPALVKLLETADYKTKKEACWAISNASSGGLQKPEIIRYLVSQGSIKPLCDLLEIADNRIIEVTLDALENILKMGEADKEARGLHINENADYIERAGGVDKIFNCQQNENEKIYEKAFNIIEQYFGEDEDALDESMAPQTAGNTFGFGSNVNQQFNFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>C5DD87</accession>
    <name>C5DD87_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0B09196p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0B09196g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. SKI2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552186.1">
        <property type="nucleotide sequence ID" value="XM_002552140.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DD87">
        <property type="interactions" value="685"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DD87"/>
    <dbReference type="GeneID" id="8291029"/>
    <dbReference type="KEGG" id="lth:KLTH0B09196g"/>
    <dbReference type="eggNOG" id="KOG0952">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000335_2_0_1"/>
    <dbReference type="InParanoid" id="C5DD87"/>
    <dbReference type="OMA" id="MCSATEF"/>
    <dbReference type="OrthoDB" id="5575at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18020">
        <property type="entry name" value="DEXHc_ASCC3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18022">
        <property type="entry name" value="DEXHc_ASCC3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000198">
        <property type="entry name" value="Activating signal cointegrator 1 complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3380.10:FF:000002">
        <property type="entry name" value="Activating signal cointegrator 1 complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000231">
        <property type="entry name" value="Activating signal cointegrator 1 complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000241">
        <property type="entry name" value="Antiviral helicase SLH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000102">
        <property type="entry name" value="RNA helicase, activating signal cointegrator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000012">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000024">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000004">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3380.10:FF:000001">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000062">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.10">
        <property type="entry name" value="Sec63 N-terminal domain-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050474">
        <property type="entry name" value="Hel308_SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004179">
        <property type="entry name" value="Sec63-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057842">
        <property type="entry name" value="WH_MER3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961:SF13">
        <property type="entry name" value="ACTIVATING SIGNAL COINTEGRATOR 1 COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961">
        <property type="entry name" value="DNA POLYMERASE THETA, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G05260)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02889">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23445">
        <property type="entry name" value="WHD_SNRNP200"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039073">
        <property type="entry name" value="BRR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00973">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158702">
        <property type="entry name" value="Sec63 N-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="6">
        <location>
            <begin position="293"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="7">
        <location>
            <begin position="517"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="6">
        <location>
            <begin position="1145"/>
            <end position="1320"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="7">
        <location>
            <begin position="1351"/>
            <end position="1546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR21748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1954" mass="221975" checksum="73CC6AB464998CEF" modified="2009-07-28" version="1">MSTQYSIDSVRSYMDAMSTMVSAAQNFKLGENIAVAPEIDNEIEKSKDESYVQLTTLSQDRNEWDDIFDSFGELSRANVTNAIESAIAEDSSSVTNELKSYMKGINSSEMDEETLLETLLLLLQSENQDSLQSELLDLLGDENIEFISFLVQNREELISIPPEEIKIALKFENSDGDFLTHREIRKQYLKNAKDAKNTELNPLQKQAEYPHVFRKSEVGSSFALSFTGQKFALPVGTTRSSFATREEIIIPAAEAGLRNSMRFKNIVIKDLDVFCTSVFTYETLNAIQSLVYPVAYETNENMLICAPTGAGKTDIALLTILNTVKQFSEVDQKGNIDIEYDSFKVVYVAPLKALAAEIVEKFSKRLAVFDMKVRELTGDMQLTKAEILETQVIVTTPEKWDVVTRKANGDNDLVSKIKLLIIDEVHLLHEDRGSVIETLVARTLRQVERSQSMIRILGLSATLPNFIDVADFLGVNRQIGMFYFDQSFRPKPLEQQLLGCRGKAGSKQARENIDNTAYEKMVDMIQRGAQVMVFVHSRKETVRSARNFISIATSHHEIDLFTEASSIREFYQKEMTKNKDKDVKELFQSGFGVHHAGMSRSDRNLTERMFKDGAINVLICTATLAWGVNLPADVVIIKGTQVYDSKKGGFTDLGISDVIQIFGRAGRPGFGSSNGTGILCTSSDRLDDYVSLITQQHPIESKLATKLVDNLNAEISLGSVTNVEEGIQWLGYTYLYVRMRKNPFSYGINWDEIRDDPQLYERRKNMIISAARRLHTLQMIVFDEVSLHFIPKDLGRISSDFYLLNESVEIFNQLCNPSATEADVLSMISMSSEFDNMKFREEEATELSRLMDNAAQCQIGGEVESAQGKTNILLQSYISRTRIFDSALSSDSNYVAQNSIRICRALFLVGVNRRWGNFAKVMLDVCKSIEKRLWAFDHPLCQFELPEPVIRQLRDKNPSMESLLDLESEELGDLVHNRKVGHKLYSILSRFPRLEISADIFPITTNVMRIHVALTPAFIWDMRIHGNAQFFWLFVEESDKSQILHVEKLILNKRQMSNPHEMDFMIPLSDPLPPQVVVKVVSDIWIGSESTQVISFQHLIRPHNETLQTKLQRLRPLPTSAMKNPLLEQIYPFRYFNPMQTMIFHTLYNTNESVFVGSPTGSGKTVVAELAMWHAFKEYPGSKIVYIAPMKALVRERVTDWRKRVTPVTGDKVIELTGDSLPDPRDVKDATIIITTPEKFDGISRNWQTRKFVQEVSLIIMDEIHLLASDRGPILEMIVSRMNYVASQTKRPVRLLGMSTAVSNAYDMGGWLGVKDNGLYNFSSSVRPVPLKMYIDGFPDNLAFCPLMKTMNKPAFMAIKQHSPDKPVLIFVASRRQTRLTALDLIHLCGMESNPRRFLNIDDEGELQYYLSQVSDETLKLSLQFGIGLHHAGLVEKDRYISHELFQKNKIQVLVATSTLAWGVNLPAHLVIIKGTQFFDAKIEGYRDMDLTDILQMMGRAGRPAFDTSGTAIVYTKQAKKMFYKHFLNIGFPVESSLHKVLDDHLGAEIASGSIANKQEAMDFLSWTFLFRRAHHNPTYYGIMEDTSAAGINKHLSNLIDDTLKNLEESSCVALRGNEIEALPFLSISSYYYISHKTIRQLLKHIKNTASFQEVLKWLSLAFEFNELPVRNGEIIMNVEMSAQSRYSVESTFVGEDELPMWDPHVKAFLLLQAYFSRVDLPIADYHQDTISVLDQALRILQAYADVAGELGYYSTVLTIIKAMQCVKQGCWYEDDPVSLLPGVGLKRITDCEFSETGFPLEKSHSMNLDKIGRLKFKQLEQVARKLNVEKEKIKDFIGECQKLPVLDNIRVEPQSDPNELVITAVHANPRHNRNFEVYCERFPKTQKELWFVIGYQGEELMMVKRFQPRRKNNGVAISGEFVVAEELHGQTLRFTIINDALDIKYDFEHTLAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DD92</accession>
    <name>C5DD92_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">serine C-palmitoyltransferase</fullName>
            <ecNumber evidence="5">2.3.1.50</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0B09306g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sphingolipid metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.50" evidence="5"/>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552191.1">
        <property type="nucleotide sequence ID" value="XM_002552145.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DD92"/>
    <dbReference type="FunCoup" id="C5DD92">
        <property type="interactions" value="943"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DD92"/>
    <dbReference type="GeneID" id="8291034"/>
    <dbReference type="KEGG" id="lth:KLTH0B09306g"/>
    <dbReference type="eggNOG" id="KOG1358">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015846_0_2_1"/>
    <dbReference type="InParanoid" id="C5DD92"/>
    <dbReference type="OMA" id="LTKYGCG"/>
    <dbReference type="OrthoDB" id="3168162at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004758">
        <property type="term" value="F:serine C-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046512">
        <property type="term" value="P:sphingosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050087">
        <property type="entry name" value="AON_synthase_class-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693">
        <property type="entry name" value="CLASS II AMINOTRANSFERASE/8-AMINO-7-OXONONANOATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693:SF2">
        <property type="entry name" value="SERINE PALMITOYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="11">
        <location>
            <begin position="159"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR21753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="552" mass="62341" checksum="49AF3DE63D23A1BA" modified="2009-07-28" version="1">MRQPPEVLPSSIPVPEAVLRSSSYVWYYCQRAAHAIPGMRYVVSYVRKSHQDDPYRTLFELFLIIYGIIYFFRKPRKQGEQAAPKLSEREVEALLDEWEPEPIAQQNIKLEWRAKSTPVLMGNSSAPTRVNLTRDEGRESYSDVLNCASTSFLQISSDPEFVKAAKDTIHQYGVGACGPAGFYGNQDVHSELEYNLARFFGTERAVLYGQDFCIAASVIPAFTKRGDVIVADDRVNVALQNALQLSRSTVYYYNHNDMEALEELLEQLQERELNEKLPSLPRKFIVTEGLFQNLGDIPDLPRLVELKRKYKYRLLVDETFSLGVLGANGRGIAEYYNMKRDASVDITIGSLAVALGSSGGFVLGDNVMSMHQRIGSHAYTFSAALPAFAVTTASRVLDHLEHDNSAVQKLRSLSQLMHQLFIDDSELSPYIEVTSSKESSILHFRLTDEYRSRVFHTTEESLFQELSAMHAKNLSEKYIEGCEREEKLLQRIVDMALKEHKVLLTRNTFVLKHETLPVTPSLKLCCSAKMTEDELRSACESVKSAVLACTSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDG4</accession>
    <name>C5DDG4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">ATP synthase subunit d, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0C00792g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the ATPase d subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552263.1">
        <property type="nucleotide sequence ID" value="XM_002552217.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDG4"/>
    <dbReference type="FunCoup" id="C5DDG4">
        <property type="interactions" value="588"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDG4"/>
    <dbReference type="GeneID" id="8291121"/>
    <dbReference type="KEGG" id="lth:KLTH0C00792g"/>
    <dbReference type="eggNOG" id="KOG3366">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080463_0_0_1"/>
    <dbReference type="InParanoid" id="C5DDG4"/>
    <dbReference type="OMA" id="VSKGRWA"/>
    <dbReference type="OrthoDB" id="35799at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015078">
        <property type="term" value="F:proton transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015986">
        <property type="term" value="P:proton motive force-driven ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008689">
        <property type="entry name" value="ATP_synth_F0_dsu_mt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036228">
        <property type="entry name" value="ATP_synth_F0_dsu_sf_mt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12700">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT D, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05873">
        <property type="entry name" value="Mt_ATP-synt_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005514">
        <property type="entry name" value="ATPase_F0_D_mt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161065">
        <property type="entry name" value="ATP synthase D chain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="5" id="KW-0138">CF(0)</keyword>
    <keyword evidence="6 12" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="8 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7 12" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR005514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR21825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="174" mass="19747" checksum="6991BAA9E69D48BE" modified="2009-07-28" version="1">MSIAKSAANKLDWASVISSLKLTGKTATQLTSFKKRNDEARRKLLELQAQPTAVDFSYYRSVLKNSEVVDKIESFYKSYKPVTVDVKKQLSTIEAFESQALENAKETEKFVAQELKDLSDTLKNIESARPFDQLTVEELAKARPDIDAKVEEMVKKGRWEVPGYKEKFGDLTVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDG5</accession>
    <name>C5DDG5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0C00814p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0C00814g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552264.1">
        <property type="nucleotide sequence ID" value="XM_002552218.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDG5"/>
    <dbReference type="FunCoup" id="C5DDG5">
        <property type="interactions" value="622"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDG5"/>
    <dbReference type="GeneID" id="8291122"/>
    <dbReference type="KEGG" id="lth:KLTH0C00814g"/>
    <dbReference type="eggNOG" id="ENOG502QTA0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006926_0_0_1"/>
    <dbReference type="InParanoid" id="C5DDG5"/>
    <dbReference type="OMA" id="CLVYCQV"/>
    <dbReference type="OrthoDB" id="2110361at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051127">
        <property type="entry name" value="Fungal_SecMet_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47424:SF6">
        <property type="entry name" value="PROLINE UTILIZATION TRANS-ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47424">
        <property type="entry name" value="REGULATORY PROTEIN GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="7">
        <location>
            <begin position="52"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="143"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="150"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR21826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="834" mass="93446" checksum="BE00B812BD2E31CB" modified="2009-07-28" version="1">MMQEVNSVAMEDAGLDQPTEYLPSASYEGVVSDSDEMSSRTSKRRIKRSGLACVRCRRRHAKCSGEKPCSTCVAANIACEYMEPNKKLVVSMKYLQKLQSDLAEMKRENIKLQASVKNIAPQDAAEAGPSNRPSDVKFHKNTVDQSTLGSSSAASASSRAATTDDEEIVPNFTERRGRLVASRTGQQYFVGSSSMTLFGMEIQSLIPKRLASRKRDGRELTSLEVDGTPRAPVQPIDEILQEEGNAYKIVLSKNDGTNDISVNFSLPSYSYAILLVDTFVTYNDGCFYFFNEGIVKEQLKAVYEGGTALPDHTLQTIWFCKVLLIFATGEMYLGTANNMNGSKLPSKAGAKLPGSGFFEQASKIFSCLFSSGRIENVTKPGGLEVMLLYAFYLQVADCTVASYFYFGQALRACLIMGWHVDAQRDSITRFELEHRRRLWWTVYMFERMLSSKAGLPLSFTDETITTELPDDFDMSSPPAGCEHYIFPEAEYITNCVKITQINAQILNKLYQRQPGSNILPVLKEIVLQLIEWRRTLSGFLQADFQRKDFKISRLSTNIFTEYFQGMNLAIRPLLFHFASIQLKRSETSDTYINLQDYSPTISALLNCSLQASINTIRSLWYLKLQNMVALFGYMDREYLFTAACTLVLFNAAFGIHDQTAEHLDHALLTFTKMRNLGNNPAGHRRAQLLRLMSSLDFKGSMKELIQNHVDDLRPDAQFENHGDKEAEIVSYVSTESDVVRISPSASVSLEGDFRADSPKEGEIVRENNASTFEPFSLDSKSDIQKFLAGLENVDTSENELWKEISHQAMWLGNAMDPTGPVGDEDTTGGLNFFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDJ4</accession>
    <name>C5DDJ4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0C01474p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0C01474g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552293.1">
        <property type="nucleotide sequence ID" value="XM_002552247.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDJ4"/>
    <dbReference type="FunCoup" id="C5DDJ4">
        <property type="interactions" value="561"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDJ4"/>
    <dbReference type="GeneID" id="8291151"/>
    <dbReference type="KEGG" id="lth:KLTH0C01474g"/>
    <dbReference type="eggNOG" id="KOG0379">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005472_0_0_1"/>
    <dbReference type="InParanoid" id="C5DDJ4"/>
    <dbReference type="OMA" id="WNRIKLQ"/>
    <dbReference type="OrthoDB" id="45365at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051285">
        <property type="term" value="C:cell cortex of cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061245">
        <property type="term" value="P:establishment or maintenance of bipolar cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23244">
        <property type="entry name" value="KELCH REPEAT DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23244:SF456">
        <property type="entry name" value="MULTIPLE EPIDERMAL GROWTH FACTOR-LIKE DOMAINS PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="463"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="725"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="898"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="980"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="814"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1020"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="10"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="20"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="463"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="537"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="591"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="628"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="647"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="677"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="737"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="901"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="987"/>
            <end position="1004"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR21855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1056" mass="117587" checksum="FD5C6DE364F2FA35" modified="2009-07-28" version="1">MAPFKFAKKISKDKAKHEPAPAPKAAPQPQPQPQIAKRFTHSAIPEPAAARIFPTPVPVQQRNVSSASALQQTEYTPWSRVKLANSPFPRYRHVASAYASEQNQVYVIGGLHDQSVYGDTWIISAHDNGSKFSSKTVDITDTTPPPRVGHASTLCGNAFVIFGGDTHKVNSDGLMDDDVYLFNINSYKWTIPRPVGPRPLGRYGHKISIIATSQMRTKLYVFGGQFDDTYFNDLTVFDLSSFRRPDSHWQFIKPNTFTPPPLTNHTMISYDYKLWVFGGDTPQGLINDVFMFDPQINDWKVMQTTGDKPPPLQEHAAVLYGDLMCVVGGKDEQDVYSNSVFFLNLISLKWFKLPSYRSGIPQGRSGHSLTLLPNHKLLIMGGDKFDYARGGEGDVHTSDVDLGCGTLLYVLDLSKLGEQCPGIFDAKRVAAPQKGVENIPTSPPQPSRNASIQQNILTPFSERYQTPNTDQGGFSAVEKFTTPKTDGVFSPRPEAEDSNTEASALGTPQDIKKPTSPVPQLKTNHWSAQSPPPRVVSLSSANESELGSSRGHDSQFSGDVSEEYGVAMIGNSPSRPVVRESILPEDETLENLPSRSYSGETTPQREDIQPVSSTPEPARPVVARDQGNLGTQVQENIASPGPENMAVRSQENTATRGQENTATRGQESMVARGQENTMKRTQENSMARGEEGLMTRSPDNSTQNGMKGVPFEIIEALRKELGDLRSEAEKSAQSASRRIEELEQENSRLKESNDAGSRLVKLQTDYDLAVADRKAHEDRVGEVEDLLSKKFLDVARLHDIIRVQSSKIGSLEGEETFKEKYEELNDKYEQLLKDHETLKAKDREEEKSLHESIAQYSVQLEQFLASRNEGQEEGNGGVRSISGHHQKVIEDLRSRMDQMVSEKQGTDSSAAELSEKHKELENKCRKLEEELKAIEYNYESSVNSVNNASRALALSQEELNKYKQESKRLADELEEMKYKSVDRRDTSLTSNDDSLLSSSGAGLAAEPKSDMRDAPYRLKIKDLKAELFIIKQERNALKDDVMELKKKLLNLENEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDL2</accession>
    <name>C5DDL2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0C01892p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0C01892g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552311.1">
        <property type="nucleotide sequence ID" value="XM_002552265.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDL2"/>
    <dbReference type="FunCoup" id="C5DDL2">
        <property type="interactions" value="60"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDL2"/>
    <dbReference type="GeneID" id="8291170"/>
    <dbReference type="KEGG" id="lth:KLTH0C01892g"/>
    <dbReference type="eggNOG" id="ENOG502RR5W">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1066355_0_0_1"/>
    <dbReference type="InParanoid" id="C5DDL2"/>
    <dbReference type="OMA" id="KHCFKLY"/>
    <dbReference type="OrthoDB" id="4062764at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR21873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="322" mass="37211" checksum="4E270A1C9FD5C396" modified="2009-07-28" version="1">MKSVYLGKRHPFIYFSKVRPGNKIIKLSMSLTDYEQKRDNAPAGNIRTLELRINPMSSSKVVKSVLGKLKSTGNVVGSYPAAHNAARGHGSEDEELKYAKPRILELKSYNPLLAKSDFVDLLPQTRFSTRAVASSVLDFEVLKVRDPRYFQFRDKYRLVFPDHKTMEAYRKYITFSRVDGVRPHFTQSSSQNPEVGYGLYARNLLAAFDSRESFFHTLRSGRGAGTPELTLETIRKTVEPLQTKSLLVWNFPSDLRPYHIMDRFWLYDIKHCFKLYWDPTTGRTLTFMAFNSEDDCARFSQNYHGVYFHENDDCKLLVEALT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDT0</accession>
    <name>C5DDT0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="15">KLTH0C03542p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0C03542g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type V subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552379.1">
        <property type="nucleotide sequence ID" value="XM_002552333.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDT0"/>
    <dbReference type="FunCoup" id="C5DDT0">
        <property type="interactions" value="1184"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDT0"/>
    <dbReference type="GeneID" id="8291243"/>
    <dbReference type="KEGG" id="lth:KLTH0C03542g"/>
    <dbReference type="eggNOG" id="KOG0209">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001828_4_1_1"/>
    <dbReference type="InParanoid" id="C5DDT0"/>
    <dbReference type="OMA" id="WYYSLFN"/>
    <dbReference type="OrthoDB" id="48943at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019829">
        <property type="term" value="F:ATPase-coupled monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015662">
        <property type="term" value="F:P-type ion transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07543">
        <property type="entry name" value="P-type_ATPase_cation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000049">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000071">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057255">
        <property type="entry name" value="2TM_P5A-ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006544">
        <property type="entry name" value="P-type_TPase_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047820">
        <property type="entry name" value="P5A-type_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01657">
        <property type="entry name" value="P-ATPase-V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630">
        <property type="entry name" value="CATION-TRANSPORTING ATPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630:SF7">
        <property type="entry name" value="ENDOPLASMIC RETICULUM TRANSMEMBRANE HELIX TRANSLOCASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23143">
        <property type="entry name" value="2TM_P5A-ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01229">
        <property type="entry name" value="COF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="23"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="56"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="197"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="220"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="399"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1055"/>
            <end position="1077"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1135"/>
            <end position="1154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1174"/>
            <end position="1192"/>
        </location>
    </feature>
    <feature type="domain" description="P5A-ATPase transmembrane helical hairpin" evidence="14">
        <location>
            <begin position="22"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="13">
        <location>
            <begin position="256"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF23143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR21941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1208" mass="133766" checksum="067D673B7EBDEA35" modified="2009-07-28" version="1">MAPRSPVDSPLVKDSRLLIKKDVFFRPYVLPFMPLYATFAHLYFKDYERYISGGEWTFVYLGGIVSLNILVMLLPYWNVNIASYFNYANASSLAEASFILIHTTPNNGADDIVEIQRVQEGGHLQVFFQFQKKRFLLNADEGVFTSPKFVVDESPKIGVFQDCKGHSGDLTHLKRLYGPNSFDIPVPSFTELFKEHAVAPFFVFQIFCVCLWLLDDLWYYSLFNLFMIIAMEAAAVFQRLTTLREFRTMGVKPYPIHVFRNGKWSSVETTELLPMDLVSITRTAEDSAIPCDLVLLEGSCIVNEAMLSGESTPLLKESIKLRPHDDLLDLEGLDKNSVLHGGTKALQVNASEKLSSIPAPPDDGALAVVTKTGFETSQGSLVRVMIYSAERVSVGNKEALYFILFLLIFAIAASWYVWTEGTKMGRIQSKLIVDCILIITSVVPPELPMELTMAVNTSLAALSKFYVYCTEPFRIPLAGRIDVCCFDKTGTLTGEDLVFEGLAGINEESEKVDALSPADDASLKTTLVVGAAHALVLLEDGEVVGDPMEKATLKACNWKVGAKDTVQKENIGKIEILRRFQFSSALKRSSSVAKHNNKMFVAVKGAPETIRERLTTVPSNYDHIYKHFTRAGSRVLALASKDLSNLSSKQVENLDRKDMEVDLEFSGFLVFQCPLKEDATETIKMLNESSHRCIMITGDNPLTAVHVAKEVGIAERECLIVDEPVDGSNHSLVLRNVNETIMMPFNPESDTFEHAEIFAKYDLAVTGHALAKLSGHHQLNELIRHTWIYARVSPAQKEFILNSLKDMGYQTLMCGDGTNDVGALKQAHVGIALLNGTEEGLKKLNDQRRIDGMKGMYDKQCQFMERWGQPNPPVPEPIAHLYPPGPSNPNYLKALEKKGLQITPEMRKLAAEAASKPVVTKKAEPSNPKASDLADMVMGSLGEADGEDAPSLKLGDASCAAPFTSKLANVSAVTNIIRQGRCALINTIQMYKILALNCLISAYSLSVIYLAGVKFGDGQATTSGLLLSVCFLSISRGKPLEKLSKERPQPGIFNIYIMGSILGQFAVHIATLVYITLEIYKLEPREPQIDLEKTFTPSLLNTGIFMIQLTQQVSTFAVNYQGEPFRESIRSNKGMYYGLLAVGGLALAGATEFSPELNATMKFVPMEDLFKIKLTATLLIDFFGSWAVELVFKYFFMDSKAADIAARD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDZ6</accession>
    <name>C5DDZ6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0C05060p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0C05060g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552445.1">
        <property type="nucleotide sequence ID" value="XM_002552399.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDZ6"/>
    <dbReference type="FunCoup" id="C5DDZ6">
        <property type="interactions" value="39"/>
    </dbReference>
    <dbReference type="GeneID" id="8291312"/>
    <dbReference type="KEGG" id="lth:KLTH0C05060g"/>
    <dbReference type="eggNOG" id="ENOG502SCD0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1496482_0_0_1"/>
    <dbReference type="InParanoid" id="C5DDZ6"/>
    <dbReference type="OMA" id="MDKTTDA"/>
    <dbReference type="OrthoDB" id="4068551at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="180" mass="19671" checksum="1D612B6239176827" modified="2009-07-28" version="1">MLSKSSSVRRIKTDKKRHSMVLTPSSLDLSSIYGTKNSNKSMDALVPERSSTQRKSNGKSRMKRSTVLLDDNMVREYNLAIQELNNFGSGAGTALPRSGASTANSSVSTSTSMSSLFSRESSMSIHDLMYQDCGEDETSTGNALEKGEVVTLNSHTAQSWSRAIDDVPLEGSQRTQLDFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>C5DED4</accession>
    <name>C5DED4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0C08294p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0C08294g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552583.1">
        <property type="nucleotide sequence ID" value="XM_002552537.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DED4"/>
    <dbReference type="FunCoup" id="C5DED4">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DED4"/>
    <dbReference type="GeneID" id="8291457"/>
    <dbReference type="KEGG" id="lth:KLTH0C08294g"/>
    <dbReference type="eggNOG" id="ENOG502RY5C">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_460089_0_0_1"/>
    <dbReference type="InParanoid" id="C5DED4"/>
    <dbReference type="OMA" id="KHDANIT"/>
    <dbReference type="OrthoDB" id="4069585at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.60">
        <property type="entry name" value="Rad61, Wapl domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031550">
        <property type="entry name" value="Rad61_Wapl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038496">
        <property type="entry name" value="Rad61_Wapl_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16997">
        <property type="entry name" value="Wap1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad61 Wapl" evidence="2">
        <location>
            <begin position="194"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="136"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="136"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR22145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="581" mass="66497" checksum="CAC7D739F142A4EF" modified="2009-07-28" version="1">MRSYGKRGRFRSLVRPHQTQLQEFSDEEESFQENTSDKAMEAVTPKSSFDADALNAGEKRNTTKEKTSGLDIFDFLDEPVRKKRKRAAQVKADSDACEEAEYDAVDAFFSSQAEQSFQSTIDDANSMLSRICGESNAKSEWESSGTQDDIDARTPDDTDNKVMYGKSRTMLHCEDEPEVTVEPPEESHDSAEPSTHHFNHLKTMGESLKYQDDLEFMMQKEAQMTLAAKTSNLLIFVLNLINNAGFFEYVMKYSRKEIWQWCLHLGNCEDPVLRHLCAFIYSKLPLDADDPMWGELNLYEFVLPLTSQKSGTLKIKGSKFTEMSYLEFLNATESKPGLYYALLLWPPFLGHQKEIDQNVVDAFTALLQNSCEYVDTILPIVEKLVSKETVEGHSTSYKNMMQALVPLFEPHARNDSLIKALVKMTNESTLVAELKDGQKTVLLTNSLEFLLAYDRFLFDEQESELLDIFVLYLGLALNLVKALKQDDAVFEDKNPLMERLKRTFQDLQAASDESNQHNFNQNIFILIYCYIAMHYKVRLQEAERAFVIPRLEAFSQEIKRYNESIYTAIAVILTPDILAPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5DED5</accession>
    <name>C5DED5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">DNA replication complex GINS protein PSF1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0C08316g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Required for correct functioning of the GINS complex, a complex that plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS complex seems to bind preferentially to single-stranded DNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the GINS complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the GINS1/PSF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552584.1">
        <property type="nucleotide sequence ID" value="XM_002552538.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DED5"/>
    <dbReference type="FunCoup" id="C5DED5">
        <property type="interactions" value="475"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DED5"/>
    <dbReference type="GeneID" id="8291458"/>
    <dbReference type="KEGG" id="lth:KLTH0C08316g"/>
    <dbReference type="eggNOG" id="KOG3303">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079191_0_0_1"/>
    <dbReference type="InParanoid" id="C5DED5"/>
    <dbReference type="OMA" id="MFCEKAT"/>
    <dbReference type="OrthoDB" id="10252587at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000811">
        <property type="term" value="C:GINS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902983">
        <property type="term" value="P:DNA strand elongation involved in mitotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11710">
        <property type="entry name" value="GINS_A_psf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21696">
        <property type="entry name" value="GINS_B_Psf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1030:FF:000003">
        <property type="entry name" value="DNA replication complex GINS protein PSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021151">
        <property type="entry name" value="GINS_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036224">
        <property type="entry name" value="GINS_bundle-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005339">
        <property type="entry name" value="GINS_Psf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056783">
        <property type="entry name" value="PSF1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12914:SF2">
        <property type="entry name" value="DNA REPLICATION COMPLEX GINS PROTEIN PSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12914">
        <property type="entry name" value="PARTNER OF SLD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24997">
        <property type="entry name" value="PSF1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05916">
        <property type="entry name" value="Sld5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158573">
        <property type="entry name" value="GINS helical bundle-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GINS subunit" evidence="7">
        <location>
            <begin position="51"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="DNA replication complex GINS protein PSF1 C-terminal" evidence="8">
        <location>
            <begin position="159"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF24997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR22146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="207" mass="23561" checksum="FBEAD07D7F567BE4" modified="2009-07-28" version="1">MYGDLGSKLVLEAKRAQQLSQSSGSDQLPLYQDDLVRNILREIAHLKKNAEILKSQQELENFDNKVAKCQYFVTLLCMERNKRCLLAYQKLRSDALDAAAWNRNGLDAMSSLNTAKDGSTNLSHHEQEYLKDYVQLITDMKSGDYTDIDLSGSLTPPSDVFIDVRVLKDAGEIQTEYGVFNLIKDSQFFVRQADVERLIQQGYLQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEG0</accession>
    <name>C5DEG0_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ATP-dependent RNA helicase DBP10</fullName>
            <ecNumber evidence="4">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">ATP-dependent RNA helicase dbp10</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ordered locus">KLTH0C08866g</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">ATP-binding RNA helicase involved in the biogenesis of 60S ribosomal subunits and is required for the normal formation of 25S and 5.8S rRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DEAD box helicase family. DDX54/DBP10 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="4"/>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552609.1">
        <property type="nucleotide sequence ID" value="XM_002552563.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEG0"/>
    <dbReference type="SMR" id="C5DEG0"/>
    <dbReference type="FunCoup" id="C5DEG0">
        <property type="interactions" value="1172"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEG0"/>
    <dbReference type="GeneID" id="8291483"/>
    <dbReference type="KEGG" id="lth:KLTH0C08866g"/>
    <dbReference type="eggNOG" id="KOG0337">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_5_1_1"/>
    <dbReference type="InParanoid" id="C5DEG0"/>
    <dbReference type="OMA" id="EDQFGMM"/>
    <dbReference type="OrthoDB" id="10261375at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17959">
        <property type="entry name" value="DEADc_DDX54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000865">
        <property type="entry name" value="ATP-dependent RNA helicase DDX54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012541">
        <property type="entry name" value="DBP10_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033517">
        <property type="entry name" value="DDX54/DBP10_DEAD-box_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF8">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08147">
        <property type="entry name" value="DBP10CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01123">
        <property type="entry name" value="DBP10CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0347">Helicase</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="13" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="8" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="20">
        <location>
            <begin position="122"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="18">
        <location>
            <begin position="153"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="19">
        <location>
            <begin position="395"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="381"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="870"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="921"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="16">
        <location>
            <begin position="122"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="39"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="394"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="873"/>
            <end position="888"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00021760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="CAR22171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="972" mass="109060" checksum="1343F7324139CAB9" modified="2009-07-28" version="1">MVKAVAKRSRQQSALSDDEDVDIAGNIALDSSDSSSSSDNDDDGSEDEAGDVQDIIEFSDEDEEEARAKAAPQKKKKNAHEGGFPALELSDNEDHDDGKDGADDVTEYFSTNNPEAAKHKKGSFASFGLSKFIVSNIAKKGFRQPTPIQRKTIPLILQKRDIVGMARTGSGKTAAFVLPMIEKLKGHSAKIGARAVILSPSRELAMQTHKVFREFSRGSDLRSVLLTGGESLEEQFGLMMSNPDVVIATPGRFLHLKVEMNLDLKSVEYLVFDEADRLFEMGFEEQLSELLAALPVARQTLLFSATLPSSLVNFAKAGLTNPVLVRLDAETKVSENLEMLFLSSKNEEREANLLYLLQEAMKIPVATEEQLLEYKAKVKNSYDSDDSDEDAKAKAKKQRKSAIRKKLPSANELPSEHATIVFVPTRHHVEYISQLLKDCGYLVSYLYGTLDQHARRQQLFNFRAGLTTILVVTDVAARGVDIPLLANVVNYSLPASSKIFVHRVGRTARAGNKGFAYSIVSENELPYLLDLELFLGKKILLTPMFEATTELLKSKWIAEGNSELTFNPPKVSYTNRMVLGACPRLDLETCGDLFKNLTQSSFDLQQLKGVAAKAEKLYFRTRPPASSQSMKRSKEVIASGWDEQNLRFGKNIEKEKLDFLAKLQNRNNKETVFEFARNQDDEMSLLMKKRRIQIAPIQRRAKERKELLEKERMAGLRHTVEDEILKGDENEVGYSVPEEVLHREFQDADELLGAQSKSKRPKSFRDPAFFLSHYAPASEIQDKQLEVTNGFTNEAANATFDLHNDDKVQNHKQVATTKWDKKRQKYVNTQGLDNKKYIMGESGHKIPASFRSGKFDEWSKARKLGPLKVGAKESSTTAKMLSNPTAEGQRSIGGRFKHNKVRAPKLPDKHRDDYHVQKKKVEKAVSSGIPVKGYNAPGMKSEMRSTQQIYKERVAKEKKRAKNARPAKKRKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEZ0</accession>
    <name>C5DEZ0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">KLTH0D10890p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0D10890g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. ABCD family. Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553183.1">
        <property type="nucleotide sequence ID" value="XM_002553137.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEZ0"/>
    <dbReference type="FunCoup" id="C5DEZ0">
        <property type="interactions" value="486"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEZ0"/>
    <dbReference type="GeneID" id="8295422"/>
    <dbReference type="KEGG" id="lth:KLTH0D10890g"/>
    <dbReference type="eggNOG" id="KOG0064">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007587_1_1_1"/>
    <dbReference type="InParanoid" id="C5DEZ0"/>
    <dbReference type="OMA" id="IHDMYLD"/>
    <dbReference type="OrthoDB" id="422637at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005324">
        <property type="term" value="F:long-chain fatty acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015910">
        <property type="term" value="P:long-chain fatty acid import into peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042760">
        <property type="term" value="P:very long-chain fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03223">
        <property type="entry name" value="ABCD_peroxisomal_ALDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050835">
        <property type="entry name" value="ABC_transporter_sub-D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384:SF67">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY D MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384">
        <property type="entry name" value="ATP-BINDING CASSETTE, SUB-FAMILY D MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06472">
        <property type="entry name" value="ABC_membrane_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="62"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="190"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="571"/>
            <end position="815"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR22745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="830" mass="94992" checksum="CD0E6B403C8DB760" modified="2009-07-28" version="1">MAETTITAQRLKELLVRLPHECIGIDLRGVARGKYAKVILKHLWWLVRNPDTSLNHRKRAQIIFITLAFSAASAASGVGYAIYTLISRLRARTFGRTPVMRRTRSQVMLENGARVMYVPYEENATNKKRILISPKSDDMYEHDKFLFKYFGKNKISQVFYSKFISQLNIISRILVPKVTDQNSILLIFQIFFLVMRTWLSLFVARLDGQIVKDIISGNSRKFLVDLLCWFLIAFPASYTNSAIKYLQRKLSLNFRINLTRYIHDMYLDKRLAFYKLTFDNQASSSVIANIDNSITNDVTKFCDAICSVFANIAKPVIDLVFFSVYLRDNLGTVGIVGIFVNYFITGYTLKRWTPPLGRLVSQRSTAEGDYYNYHLNLINNNEEIAFYHGTKVERTKVNALYDHLMDQMLLVDRVKVNYNILEDYVLKYTWSALGYAFASIPIVIATFSSGVNNEDVNMREFIVNKRLMLSLADAGGRLMHSIKDIAQLTGYTNRIFILLSVLHRVHSNVYNYGATVEEGEDSSGHNSLTSSDIVRGTLQRNFNGIRLENIDVVIPSKYGKDGTRLINKLSFQVPPVITLGNGNSDSNLNINKKLAFQGPGSSMLILGPNGSGKTSIQRIIAEIWPIYNKNGLLSIPPASDLFCVPQKPYFIQGGTFREQIIYPMSADQYFDMGYRDKELTHILGEVKLDYLLKRDKGWRYLDAVAEWKDVLSGGERQRINFARIMFHKPRFVVLDEATNAISADMEDYLFGLLKRYRFNFITISQRPSLIKYHDLALDISTDGTWRLQNLGTDEVITSIEKEIEMLENKLKNVNVWEGERAALMRKLAHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>C5DF06</accession>
    <name>C5DF06_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Aspartate protease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">KLTH0D11264g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553199.1">
        <property type="nucleotide sequence ID" value="XM_002553153.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DF06"/>
    <dbReference type="FunCoup" id="C5DF06">
        <property type="interactions" value="451"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DF06"/>
    <dbReference type="MEROPS" id="A01.018"/>
    <dbReference type="GeneID" id="8295439"/>
    <dbReference type="KEGG" id="lth:KLTH0D11264g"/>
    <dbReference type="eggNOG" id="KOG1339">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013253_3_4_1"/>
    <dbReference type="InParanoid" id="C5DF06"/>
    <dbReference type="OMA" id="KYDHDAS"/>
    <dbReference type="OrthoDB" id="771136at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05488">
        <property type="entry name" value="Proteinase_A_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000036">
        <property type="entry name" value="Vacuolar aspartic protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000002">
        <property type="entry name" value="Vacuolar aspartic proteinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033819">
        <property type="entry name" value="Saccharopepsin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF51">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="8 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 13" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950354" description="Aspartate protease" evidence="14">
        <location>
            <begin position="23"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="15">
        <location>
            <begin position="103"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="121"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="306"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="134"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="340"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAR22761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="417" mass="44679" checksum="A8CB23225ABC5F99" modified="2009-07-28" version="1">MHLPSLLSLATLLVASFNSAEAKVHKSKVYKSKQHNDGSELGVMMSVANLHQKYLSQFSKAYPEVDFASHAGSGIGLGAVEQDVLSAMGGHDVPLSNYLNAQYFTEITLGTPPQSFKVILDTGSSNLWVPSDECGSLACFLHSKYSHDASSSYKANGTNFAIQYGSGSLEGYISQDTLSIGDLTIPKQDFAEATSEPGLAFAFGKFDGILGLGYDTIAVDKVVPPVYKAINDGLLDEPRFAFYLNNADDSEESTGEVTFGGIDSSKYKGNITWLPVRRKAYWEVKFDGIGLGDEYAELEGTGAAIDTGTSLIALPSGLAEVLNAEIGAKKGWSGQYTVDCESRDQLPDLTFTFNGKNFTISAYDYTLEVSGSCISAFTPMDFPEPVGPLAIIGDAFLRKFYSVYDLGNNAVGLAQAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>C5DF27</accession>
    <name>C5DF27_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0D11726p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0D11726g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553220.1">
        <property type="nucleotide sequence ID" value="XM_002553174.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DF27"/>
    <dbReference type="FunCoup" id="C5DF27">
        <property type="interactions" value="39"/>
    </dbReference>
    <dbReference type="GeneID" id="8295460"/>
    <dbReference type="KEGG" id="lth:KLTH0D11726g"/>
    <dbReference type="HOGENOM" id="CLU_683471_0_0_1"/>
    <dbReference type="InParanoid" id="C5DF27"/>
    <dbReference type="OMA" id="CNDEEVY"/>
    <dbReference type="OrthoDB" id="4035871at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="369"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="404" mass="45964" checksum="53E752D07E9D6A47" modified="2009-07-28" version="1">MICVNSLRAYLRHNLPRELRFYVARGPTHPFSSLSSISDTGNIDDIAAVAFTKKLKNVVRHSKIPEQNSQLSREIIALCESSGLKCDFLTSSKIKRLYNMLLQGKISTLQIMNALKSARNQKMVNVMDPKKSIDNQKDKKADISQYEFLQINPASSEKFGKILDEMGCSETRKPFKQKVGTRRENEVPPLKGSDEIDLGVLHSFLQSAEKQKKEQKQFAWNQARAYEWNEKNSPAAFSPGMMLFDSTYTPLRSKLMRNFRGSNVRGQGTGTVKTAFTYTTEMLFYNLKDSTTHVMLWKNQGNNVHIEYRDLFTVINGSQRPPEQLLKVITNFEGKDWELTGEATIDGVRQIVFKRDTTKHSSNRVSSKIFNMTLLSVFLGFAAYGAIQKYRSTRYTVTTSAEPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5DF37</accession>
    <name>C5DF37_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0D11946p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0D11946g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL24 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553230.1">
        <property type="nucleotide sequence ID" value="XM_002553184.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DF37"/>
    <dbReference type="FunCoup" id="C5DF37">
        <property type="interactions" value="190"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DF37"/>
    <dbReference type="GeneID" id="8295470"/>
    <dbReference type="KEGG" id="lth:KLTH0D11946g"/>
    <dbReference type="eggNOG" id="ENOG502QUDZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046293_0_0_1"/>
    <dbReference type="InParanoid" id="C5DF37"/>
    <dbReference type="OMA" id="KEWKFIP"/>
    <dbReference type="OrthoDB" id="359154at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003256">
        <property type="entry name" value="Ribosomal_uL24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12903">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOMAL PROTEIN L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22682">
        <property type="entry name" value="Ribosomal_uL24m-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="293" mass="33484" checksum="B6CA093A77FC7EC3" modified="2009-07-28" version="1">MSGYQHLSKAGSRFLKQVERRPKHLVDHFKKFEEKSMPSFLVPSIPRVENDQKFQSVREWVFMPGDRVVITRGRWKGQISVIQQHDKETNGFILDENGPTKTVPVPKQFWSEGQNSHMVTFPIAVEKKDLKLVADIDDPAAPGQTKTVAVRDIVFKGSYFDENYKQRMPFRCVSGQEDLVIPWPRPEPKADAELATPAEVAREQTFWVESIAKNPIPEGALLTVRNPHSKWRRGTLSARDIAKLVAPKMPLTKTKKAYIAEQKELAAAPRRKLTSEDKELIGSKIFQHLKQHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DF45</accession>
    <name>C5DF45_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Tubulin-specific chaperone A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0D12122g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Tubulin-folding protein; involved in the early step of the tubulin folding pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Supercomplex made of cofactors A to E. Cofactors A and D function by capturing and stabilizing tubulin in a quasi-native conformation. Cofactor E binds to the cofactor D-tubulin complex; interaction with cofactor C then causes the release of tubulin polypeptides that are committed to the native state.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Cytoplasm</location>
            <location evidence="2 9">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the TBCA family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553238.1">
        <property type="nucleotide sequence ID" value="XM_002553192.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DF45"/>
    <dbReference type="FunCoup" id="C5DF45">
        <property type="interactions" value="716"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DF45"/>
    <dbReference type="GeneID" id="8295478"/>
    <dbReference type="KEGG" id="lth:KLTH0D12122g"/>
    <dbReference type="eggNOG" id="KOG3470">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130569_2_0_1"/>
    <dbReference type="InParanoid" id="C5DF45"/>
    <dbReference type="OMA" id="EECEMMI"/>
    <dbReference type="OrthoDB" id="296187at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048487">
        <property type="term" value="F:beta-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007023">
        <property type="term" value="P:post-chaperonin tubulin folding pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007021">
        <property type="term" value="P:tubulin complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.90:FF:000010">
        <property type="entry name" value="Tubulin-specific chaperone A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004226">
        <property type="entry name" value="TBCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036126">
        <property type="entry name" value="TBCA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21500">
        <property type="entry name" value="TUBULIN-SPECIFIC CHAPERONE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21500:SF0">
        <property type="entry name" value="TUBULIN-SPECIFIC CHAPERONE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02970">
        <property type="entry name" value="TBCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46988">
        <property type="entry name" value="Tubulin chaperone cofactor A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6 9" id="KW-0493">Microtubule</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR22800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="107" mass="12414" checksum="3C4C75BD9D34603F" modified="2009-07-28" version="1">MAPSQLEIKVRSLQRLVKEEKYYQQELSDQKAHVENLKANSEVDPYDLKKQVEVLQDTQRLLPALYEKIGQFKEDLVQFTKTYSGSEDLGSSKQVLKEAADLLEQKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>C5DF73</accession>
    <name>C5DF73_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">KLTH0D12760g</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the peptidase C19 family. UBP8 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553266.1">
        <property type="nucleotide sequence ID" value="XM_002553220.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DF73"/>
    <dbReference type="FunCoup" id="C5DF73">
        <property type="interactions" value="574"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DF73"/>
    <dbReference type="MEROPS" id="C19.087"/>
    <dbReference type="GeneID" id="8295506"/>
    <dbReference type="KEGG" id="lth:KLTH0D12760g"/>
    <dbReference type="eggNOG" id="KOG1867">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008279_11_2_1"/>
    <dbReference type="InParanoid" id="C5DF73"/>
    <dbReference type="OMA" id="KSHNFWL"/>
    <dbReference type="OrthoDB" id="289038at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF33">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00290">
        <property type="entry name" value="ZnF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50271">
        <property type="entry name" value="ZF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBP-type" evidence="17">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="16">
        <location>
            <begin position="136"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAR22828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="453" mass="51130" checksum="193718EBC9284ED7" modified="2009-07-28" version="1">MSCQHLDQIFRNEKTRDGVLRAYNMIRYIIEHSSFQNRHLHSMRCSECLEINCGSTFMCLQCGVCGCWNKSHFALHSKKAGHIFGVNSSNGLLFCFKCGDYVGGNETLINSTLVKYWDDISVKSSLPVAKGRDGLCGLVNMGSTCFMSSIIQTLVHNPYVLKQCMNQNHYSRCDIQDSSSCFSCALDQIVMNFYGPTSDATFTSQQRGFVDLLLCSWKTNKNLAGYSQQDAHEFLQFFLNQLHADHVRVAQTNVKAENCNCILHTAFQGSLRSSILCTDCQDGSNITFDPMMDLSLDIKNKSTLVECLQSFHKGERLTDFNHHCSSCGSVQNPIKQMTIAKLPPVLVLQLKRFEHLMNGNNVKLNGEITYPAYLNMAPFCHHSEARSVPDMVYELVAVISQEGSVNQGHYTCMCKVPGGQWFRFNDTMIVSITEEDVLRQQAYLLFYVIRQIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFE5</accession>
    <name>C5DFE5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0D14454p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0D14454g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553338.1">
        <property type="nucleotide sequence ID" value="XM_002553292.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFE5"/>
    <dbReference type="GeneID" id="8295582"/>
    <dbReference type="KEGG" id="lth:KLTH0D14454g"/>
    <dbReference type="eggNOG" id="ENOG502SF1Z">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2812839_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFE5"/>
    <dbReference type="OrthoDB" id="4034162at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR22900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="76" mass="8788" checksum="B343ACB38431EEE8" modified="2009-07-28" version="1">MYPTVVLRNAQAASKAPSPHAMIYRRFAKPVGKMLTLCVGSYYSLCYAWEWLEKRERALESQKQQRRRSSELPGPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFG0</accession>
    <name>C5DFG0_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">E3 ubiquitin protein ligase</fullName>
            <ecNumber evidence="14">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">KLTH0D14784g</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 14">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the BRE1 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="14"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553353.1">
        <property type="nucleotide sequence ID" value="XM_002553307.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFG0"/>
    <dbReference type="FunCoup" id="C5DFG0">
        <property type="interactions" value="993"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFG0"/>
    <dbReference type="GeneID" id="8295597"/>
    <dbReference type="KEGG" id="lth:KLTH0D14784g"/>
    <dbReference type="eggNOG" id="KOG0978">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019713_1_0_1"/>
    <dbReference type="InParanoid" id="C5DFG0"/>
    <dbReference type="OMA" id="ERHRACR"/>
    <dbReference type="OrthoDB" id="654191at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033503">
        <property type="term" value="C:HULC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16499">
        <property type="entry name" value="RING-HC_Bre1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000414">
        <property type="entry name" value="E3 ubiquitin protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013956">
        <property type="entry name" value="E3_ubiquit_lig_Bre1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23163:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE BRE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23163">
        <property type="entry name" value="RING FINGER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08647">
        <property type="entry name" value="BRE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="11 14 15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 14" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9 14" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 13 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="17">
        <location>
            <begin position="611"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="142"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="229"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="346"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="469"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="560"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU365038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAR22915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="663" mass="75340" checksum="CFC008E020A7D486" modified="2009-07-28" version="1">MSEEPTPKKAKLDWGLTDPSEPLTRKDVIAFQKEALFRAINKHRCREKSLEKQLETGELQSAELREQYASLYAMITALAGKVLTLSKEDLEAQKLCGAILEGGNKEVPGLAADFTALVEKHISHDAASPRQDSEEVTWNKQLHQLEATKAELSAHNRQLKKEISAIREHYQDLIRRYDREDSDTVRRVFKLQESPGESSAKEVAEISPANQSEVANAMTGDEKEEAVSKMEHELQIQDLNNQIESLKQTLKDLESWKASSETEIAKLRTELASNSSACEQTSRSIQNDRETLLHKLDLVTKENAELAQANDLYLSKFQQMVQDKEIFTNKLSLEFQTAQDALKKHNAMLEKDLVRIRATRDELTSRIAILESQKPKSELLADLQKVLDLQKERIEKMENKPVETTNDAVMKELQDLERAFKELTQLAHKKYSDHISQESIVSKLTVEKTKADQKYFTAMRSKDSILIENKNLSKNLHKSNELINQLKEIERTLQSKVESLNKQIHLSQSNEKRLMESSKAVSLKIMDLTSELGKTKKVVESLQGERAKHIEQDTRLESNIHSLQSQNSALKLQLSQAENKAKKLHKSLISNGGDNSIIAEELDNFRTIVYCSLCSKNWKNTAIKNCGHVFCEECCKERLAARMRKCPTCNKPFGSADLLVVHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFK0</accession>
    <name>C5DFK0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0D15752p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0D15752g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553393.1">
        <property type="nucleotide sequence ID" value="XM_002553347.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DFK0">
        <property type="interactions" value="170"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFK0"/>
    <dbReference type="GeneID" id="8295640"/>
    <dbReference type="KEGG" id="lth:KLTH0D15752g"/>
    <dbReference type="eggNOG" id="KOG4305">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002884_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFK0"/>
    <dbReference type="OMA" id="HEIITWE"/>
    <dbReference type="OrthoDB" id="660555at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001180">
        <property type="entry name" value="CNH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057283">
        <property type="entry name" value="RGF3_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052233">
        <property type="entry name" value="Rho-type_GEFs"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46572">
        <property type="entry name" value="RHO1 GDP-GTP EXCHANGE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46572:SF1">
        <property type="entry name" value="RHO1 GUANINE NUCLEOTIDE EXCHANGE FACTOR TUS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00780">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23582">
        <property type="entry name" value="WHD_RGF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00036">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50219">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="4">
        <location>
            <begin position="481"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="728"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="domain" description="CNH" evidence="5">
        <location>
            <begin position="954"/>
            <end position="1297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="165"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="794"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="50"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="95"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR22955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1320" mass="149075" checksum="FDF2EFD4AADA277C" modified="2009-07-28" version="1">MARSRWVDQDIDNAKPKLPALPPLVTAQVTPERTLRDRDQVPIGWSQIKSGAATLSSGPSPSSSSDASAFSPHSASSVRTRRPPPPLVGAESLSPRINNNSAAQRAQRTPASPLSYKSPFVGEQELFEPITRSNSKARVFKSPFVGDSSVLMPAALEGGKLEWTNQIDNGSPPEVVFSNDPRKSDEMKGLKALPPLPPRNQNSALLSVDKLAGDNVAQLNLGVGPLEKSDKNFEEPLKPFTNDMRHRSDKRTISDSISSYYSSSNYAFNDELGKHSSFNSIAGNRPLQHVPSVTAPTQPFSIDLLDENKLYQCYTVSLLSDIYEWLLKVYFEWFNEYVFHKIEFFQMVQLLLEFQLPVSCDQDIVDSNVDRVIESLVLQGAVRFEGDTVNSSSDEITIIVAGLAVQGVFTALLPCYSYEGQRILSNQDPHCYCPRCPSKFMKQSRPVLKVSEIINKSVGLWTDYWKLTPEELTEINPREVKRQSLFFDLIILEERSLNLANAAVEIYGSQFDPSLLPEDPNFKKLAFDIFEPMVELHKEYLLTPLFWKLKSRGKFIDGVGKFYLQWCNSARELYVQYAESMATVHEIISWEKAHKTRFSLWLKDIDNSPEISRSKLYHDVIFFGGFFKALQNIPVTLSSILKCTDQSMEDYEYLKLAIADIKALNATVDTVHGNAIDHRRVVRFARQLVLGSGSNSSTVGYVNINDGNPNAQSAQEKLDLRLNEKSRKLLNSGPVHKKRELWIENAQLYLVLLDNYLLITEDVTRNNETKYKLCERPIPIDYLSLESKEDLKLSKFSSDQQEHSSLESRSNSRPAISAAKSNRLSLYGSRFSPLHSKTLHNEIQALQNKNESSDHQESSITFKVRNTATNESFTFSTSTIEERDAWVSSLVSCFKAHHAKNNSRIFLLSCLTDMFSYEEGQAPTNLPVVHEGSAIDSALKAYYAKSPPEKDGISAEIRCTVLLPYEGKTFVLCATNQSIYMRCVDYSNKWKNILILSKVTRMQANARLGLFFVLADRRLCYFNIPSIVCAYFGASEYLSQKQITGIILQEKVGFFKVVDDFGNSRQLFFERKGKIVVLTPEFDRLTKKLKFFKFYKEYRLPSSANGLVSTDVKDIAVFPKSFIVCSSKGALLFNDSFNDVGLFLPSFLNDKTISDHQHHFTHHAVKSYKDSLSKMDSSKQKMASYVKTDILNRKTSCVGCFQLSDNDFIIIYDEAVLRVNQNGEIPDWKADILVLDFYCLAASLTEEFLILFGENLVQIYDLNYAGIILNNKLSRLTPIQIIKSKKIKLIHSDRTESPTITLSHPSIPGRQLVLAFQLEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFK6</accession>
    <name>C5DFK6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0D15884p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0D15884g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
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        <property type="taxonomic scope" value="Eukaryota"/>
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        <property type="component" value="Chromosome D"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="375"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22961.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
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    </evidence>
    <sequence length="434" mass="48316" checksum="F711DB2F1D5D2937" modified="2009-07-28" version="1">MQLEEVLIQKAKIEEFLGELAAAHHKYLLQIHNPDVLDRLRRDILTCFSDICRLLSILVAADGEGSVRGEIDRLKKTASKLDRLSQRERSLRAQAANWSTTALHEASPEPPAKPAERHELHSTYRLYLNRYLDDVGVANTSLADGQETRASRESEPENPAPITVLKRIALLQACKERADREVSQLESLLAAFKKDQELVSEEMRRQQSHVDSAKLALESDLQHVRHSQERVLAKLGISAKTNRQNASLLSNFGTLGRREGSPVLHDQLGYAKSYLETKEDVLVADLKTFKNELHPAEHSRNTWEDSLSQISKLEASLKSILIRNPDTPPRVLSTEINAVVGHLEDASSGTSSSLLKYCLGSEIEVLKKATEELGPLSTRQSPRRPYKERGGTLLLNGASRPQTGLNIDIVTSKEAPFTPNGSPSIGEIKMQKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFP3</accession>
    <name>C5DFP3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">3-hydroxy-3-methylglutaryl coenzyme A reductase</fullName>
            <shortName evidence="11">HMG-CoA reductase</shortName>
            <ecNumber evidence="11">1.1.1.34</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0D16786g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">HMG-CoA reductase; part of the first module of ergosterol biosynthesis pathway constitutes by the early steps of the pathway, conserved across all eukaryotes, and which results in the formation of mevalonate from acetyl-coenzyme A (acetyl-CoA). HMG1 and HMG2 catalyze the reduction of hydroxymethylglutaryl-CoA (HMG-CoA) to mevalonate that is the rate-limiting step within the first mosule. The first module starts with the action of the cytosolic acetyl-CoA acetyltransferase ERG10 that catalyzes the formation of acetoacetyl-CoA. The hydroxymethylglutaryl-CoA synthase ERG13 then condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA. The rate-limiting step of the early module is the reduction to mevalonate by the 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductases HMG1 and HMG2 which are derived from a single ancestral HMGR gene by gene duplication.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(R)-mevalonate + 2 NADP(+) + CoA = (3S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:15989"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:36464"/>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.34"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="11">Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Endoplasmic reticulum membrane</location>
            <topology evidence="2 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus envelope</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the HMG-CoA reductase family.</text>
    </comment>
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        <property type="protein sequence ID" value="CAR22998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <property type="reaction ID" value="UER00103"/>
    </dbReference>
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        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004420">
        <property type="term" value="F:hydroxymethylglutaryl-CoA reductase (NADPH) activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="GO" id="GO:0015936">
        <property type="term" value="P:coenzyme A metabolic process"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="P:isoprenoid biosynthetic process"/>
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    <dbReference type="CDD" id="cd00643">
        <property type="entry name" value="HMG-CoA_reductase_classI"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3270.10">
        <property type="entry name" value="HMGR, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HMG_CoA_Rdtase"/>
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        <property type="entry name" value="HMG_CoA_Rdtase_eu_arc"/>
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        <property type="entry name" value="HMG_CoA_Rdtase_N"/>
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        <property type="entry name" value="HMG_CoA_Rdtase_NAD(P)-bd_sf"/>
    </dbReference>
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        <property type="entry name" value="HMG_CoA_Rdtase_sub-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053958">
        <property type="entry name" value="HMGCR/SNAP/NPC1-like_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000731">
        <property type="entry name" value="SSD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00533">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10572">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL-COENZYME A REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10572:SF24">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL-COENZYME A REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00368">
        <property type="entry name" value="HMG-CoA_red"/>
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    </dbReference>
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        <property type="entry name" value="HPIH"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF12349">
        <property type="entry name" value="Sterol-sensing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00071">
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    </dbReference>
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        <property type="entry name" value="NAD-binding domain of HMG-CoA reductase"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00318">
        <property type="entry name" value="HMG_COA_REDUCTASE_2"/>
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    </dbReference>
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        <property type="entry name" value="HMG_COA_REDUCTASE_3"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50065">
        <property type="entry name" value="HMG_COA_REDUCTASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50156">
        <property type="entry name" value="SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 11" id="KW-0521">NADP</keyword>
    <keyword evidence="8 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="188"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="219"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="244"/>
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    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="498"/>
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    </feature>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="538"/>
            <end position="586"/>
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    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004259"/>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00007661"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00056313"/>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU361219"/>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1046" mass="113656" checksum="A0236A70864BB519" modified="2009-07-28" version="1">MPLLFRQFKSLAKPFAYISKLSARQPIHVILITISLAAVAYLSVVRSYFNGWQLDTKSIFAGNSDADQLQDECIHYLHAGDAGAWTKLSGEEVASAVFSEHYYLTQLDFKSHNRTLSLPELDNVVVESNGTKFVLQENLTLPKVLHSKDGTTWTLRPHRFRLYDIKNGIISTISTLLGKIQDAEMFDVFIIGTAYVAMLYTVAGLFLEMRKKTESKFWIALAAIVGSTSALCLGLYTSQVLLELPVTALSLIEGIPFVVVIVGFNHKIRLASYLLNYLKKYGFSQKYDASELVFHAMCEEGARLLQDHLLCIVAFLGCSLYAHDLTALRNFCILASLVLFYDLILTCTYYASVLALKLEINIIHRSTIIKETLEEDGVIKSTAESVSVAETQSKASILTSNTSVILLKLLVVIGFISFNFYSFGARWTYQTFSALYSKPSDPVLPDFISGSLGKSGSSSVLISLVPAQYYQPMKVYFQVEDLILSTLRYVSVSIRDRFISKLVLFALVVSASINIYLLNAGRIHTEFTAKQLQKLNKKKSSAASKQHATEKTNGKSVAPAPKKPISPPLTSKSSSEEESESDLDEKTVRPLETLAEIMKSGGVKSMNNREVASLVVGGKLPLYALEKQLGDTTRAVVVRRRALARLADAPVLETDRLPYRHYDYDRVLGACCENVIGYMPLPVGVIGPLVIDGVPYHIPMATTEGCLVASAMRGCKAINAGGGATTVLTKDGMTRGPCVRFPTLARAGACKIWLDSEEGQNRVKKAFNSTSRFARLQHCQTALAGDLLFIRFRTTTGDAMGMNMISKGVEFSLKQMVEEFGWDDMSIVSVSGNYCTDKKPAAINWIEGRGKSVVAEAIIPGDVVRKVLKSDVKALVELNISKNLIGSAMAGSVGGFNAHAANLLTAVYLALGQDPAQNVESSNCITLMNEVNGDLKISVSMPSIEVGTIGGGTILEPQSAMLDLLGVRGPHPTAPGDNARQLAKVVACAVLAGELSLCSALAAGHLVQSHMILNRGKSTPATPATPATPQEGVKRLKEGAVNCIKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFP4</accession>
    <name>C5DFP4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0D16808p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0D16808g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553437.1">
        <property type="nucleotide sequence ID" value="XM_002553391.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFP4"/>
    <dbReference type="GeneID" id="8295686"/>
    <dbReference type="KEGG" id="lth:KLTH0D16808g"/>
    <dbReference type="HOGENOM" id="CLU_2306636_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFP4"/>
    <dbReference type="OrthoDB" id="10297058at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950449" evidence="1">
        <location>
            <begin position="18"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="100" mass="11039" checksum="2DBC996C5BE5FA51" modified="2009-07-28" version="1">MKFSVLFSVFCASVASGMCIVRFDQDFPIPVDTTQPVGDFMCNHAELWLHKFSNGTVALESIDDNSIVQIIPDDSVFAQTAEDSGYLQSTDDDELEWAPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFS7</accession>
    <name>C5DFS7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0D17578p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0D17578g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TTI2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR23032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553470.1">
        <property type="nucleotide sequence ID" value="XM_002553424.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFS7"/>
    <dbReference type="FunCoup" id="C5DFS7">
        <property type="interactions" value="19"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFS7"/>
    <dbReference type="GeneID" id="8295720"/>
    <dbReference type="KEGG" id="lth:KLTH0D17578g"/>
    <dbReference type="eggNOG" id="ENOG502QU79">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054067_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFS7"/>
    <dbReference type="OMA" id="SSNLWWI"/>
    <dbReference type="OrthoDB" id="6417021at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110078">
        <property type="term" value="C:TTT Hsp90 cochaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018870">
        <property type="entry name" value="Tti2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10521">
        <property type="entry name" value="Tti2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00034736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="420" mass="47504" checksum="2A0AB158AA8E3909" modified="2009-07-28" version="1">MDRDKSITTFIGNVGSSLDYKPTAEELSDVCDQLLKEHTQMSSIGIAAAIKSITFYCLDKRLNGEIRQGCLECIKAVMNAEVWAILAEDLRAMLIQLRNKQISAAGRLKGSNTLTLRPTKGFSLQEDKVRNAWQENGGKRSIPLFYVVLAHIEHRNISSNLWWVTPGILNLMDDTTDLEGIKLQGVVLLRQFLTESIDLTDANHFDFANTGLFEIFDSSLKSLWYHFPPSTEPILTAKIWDLVFSTYIPLCKAQFAKDCASYDLHVSQFMSEILLQATLPRIAADYKDLTVQVLQYMDTIFDILGPKSVVHLQRVIFNIGEHIIRNAFITLFMPLVHQVLSTLTHLVSVCPEERIVAHKYDLLACALILSEKCRLEGTLDGRTSAHLRKFLQALQQNGCIWDTEERQKLTSMVAHSFELP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFT9</accession>
    <name>C5DFT9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0D17886p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0D17886g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the CTF8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR23044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553482.1">
        <property type="nucleotide sequence ID" value="XM_002553436.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFT9"/>
    <dbReference type="FunCoup" id="C5DFT9">
        <property type="interactions" value="72"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFT9"/>
    <dbReference type="GeneID" id="8295734"/>
    <dbReference type="KEGG" id="lth:KLTH0D17886g"/>
    <dbReference type="eggNOG" id="KOG4487">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090690_1_0_1"/>
    <dbReference type="InParanoid" id="C5DFT9"/>
    <dbReference type="OMA" id="QRPLPIM"/>
    <dbReference type="OrthoDB" id="121932at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031390">
        <property type="term" value="C:Ctf18 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018607">
        <property type="entry name" value="Ctf8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28605:SF1">
        <property type="entry name" value="CHROMOSOME TRANSMISSION FIDELITY FACTOR 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28605">
        <property type="entry name" value="CTF8, CHROMOSOME TRANSMISSION FIDELITY FACTOR 8 HOMOLOG (S. CEREVISIAE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09696">
        <property type="entry name" value="Ctf8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR23044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="129" mass="14598" checksum="7128317873226D0F" modified="2009-07-28" version="1">MPAVEISVSKLGSLFQEEGKISVYTRLGNTLLEIQGELEHPLEVPQGNTNGKFTKYRGEDIVRFGLLSVDEDQKRATLFVGEKQRLLGTIVKLETPLGLLKFDHESVSVELQDIIRYKIIFKDRPLPVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFW8</accession>
    <name>C5DFW8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Quinate transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0D00572g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552748.1">
        <property type="nucleotide sequence ID" value="XM_002552702.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFW8"/>
    <dbReference type="GeneID" id="8294968"/>
    <dbReference type="KEGG" id="lth:KLTH0D00572g"/>
    <dbReference type="eggNOG" id="KOG0254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_30_12_1"/>
    <dbReference type="InParanoid" id="C5DFW8"/>
    <dbReference type="OMA" id="PADHIYM"/>
    <dbReference type="OrthoDB" id="508119at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF34">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0672">Quinate metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="131"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="164"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="198"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="291"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="329"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="358"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="398"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="431"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="460"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="12">
        <location>
            <begin position="30"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="528"/>
            <end position="553"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00043213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR22310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="553" mass="60971" checksum="A6751E238BAA507C" modified="2009-07-28" version="1">MKAFKLKNIFAKAEDRPTPPQIYNWRIYGTALVASTASVLIGYDTGFVGGCFANKYFLANFGLVEGEARTDDTISNLISCFHAAAFFGALFSYPLSHYWGRKRALIIAAAVATVGAAVMLGSVTGHLAPMYIGRVFTGLTVGASTNLTVVYLSEVSPAPIRGQIIAAFEIGWRVGDLVGFWINYGINQHIAPGNKQWIIPVGVQVIPAALFFIGSLIMCESPRWLFQMHRDQEAIKNLCFLRNLPDDDGYMIWEINSIRESIATQNEEIGPGLLDPAREVFIRNRKYAKRLVISSILLVIPNFMGIQSINYYSPKIFSSIGVKGTNATLFSSGMFGVVKFICTFIYILFIVDNLGRRKAFLASASMCSLCFWYLGAYLKVNDPTKPGATPKPGGKAAIGFMFIWTASFILAFSGGPFVWSSEVFEQNIRTFVQAINAAMSWVPIFIMTRLTTTMIDHMKYGIFFFFAAIAALAIPFVFFFVPETKGIALEDMDQLFARGLPAYKAHKVVTSKSEDTVLEIQNSASQFFSDQGSKKGTQKLVEDADVGESASKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFY5</accession>
    <name>C5DFY5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Eukaryotic translation initiation factor 3 subunit B</fullName>
            <shortName evidence="6">eIF3b</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Eukaryotic translation initiation factor 3 90 kDa subunit homolog</fullName>
            <shortName evidence="6">eIF3 p90</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Translation initiation factor eIF3, p90 subunit homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">PRT1</name>
        <name evidence="9" type="ordered locus">KLTH0D00946g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome.</text>
    </comment>
    <comment type="function">
        <text evidence="6">RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 7">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6 7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the eIF-3 subunit B family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552765.1">
        <property type="nucleotide sequence ID" value="XM_002552719.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFY5"/>
    <dbReference type="FunCoup" id="C5DFY5">
        <property type="interactions" value="1443"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFY5"/>
    <dbReference type="GeneID" id="8294985"/>
    <dbReference type="KEGG" id="lth:KLTH0D00946g"/>
    <dbReference type="eggNOG" id="KOG2314">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011152_4_0_1"/>
    <dbReference type="InParanoid" id="C5DFY5"/>
    <dbReference type="OMA" id="LWGGPQF"/>
    <dbReference type="OrthoDB" id="10250414at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12278">
        <property type="entry name" value="RRM_eIF3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03001">
        <property type="entry name" value="eIF3b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011400">
        <property type="entry name" value="EIF3B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034363">
        <property type="entry name" value="eIF3B_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013979">
        <property type="entry name" value="TIF_beta_prop-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14068">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 EIF3 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14068:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08662">
        <property type="entry name" value="eIF2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036424">
        <property type="entry name" value="eIF3b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6 7" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5 6 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="38"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR036424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR22327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="739" mass="84312" checksum="83BF8518BC010D21" modified="2009-07-28" version="1">MATSALEDIRIEDIPVDDVDFSDLEKKYEVESPVNFDNFVVVDGAPVAPESKVPVLTKVLTKLFSQAGSVVDINLPLDEEKKTTKGYLFVEFDSPASARKAIKLLNGKKLDVKHRLFVNSLTDVEKFGTEDFDTEFHEPKLPEFQKTDYLQSWLQDEQGRDQFVLQKGELTGVFWNRNTQQPENAVEPRTNWSNTTVKFSPKGTYLFSFHNQGVASWGGRNFERLRRFFHPQAKALAISPTEKYLVTLSPEPIVVPEDAPEDFPFGPESNGHQLCVWDIATGLSVKTFALPPNQRLHWPMIKWSFDDKYCARLGPNALAVYDAENNFELLGGRIMKIEGIQDFSFAPAGAKLAVNRNGELSSVLAFWTPESNNQSCKATVMEIPTRRVLRTVNLVQVSDVTLHWQNQAEYLCVKVDRHTKSKKTFFSNLEICKLTEKDVPVEKVEMKDLAVRFAWEPKGHRFVTISTTETADDNPSIPKNVVTFFAPEQKEKTNVAVKAEPKKWKAFASVPGKFSNTISWSPAGRFVVVATLVTPDTRKADFDFYDFDFTGDKNDNDSDDVAANLKAVAHPEFYSATDMQWDPSGRFVAVWSSAVKHKLENGYKVFNVAGECVREEMIEAFKNFVWRPRPESLLTNGEKKKVRKNLREWSAQFEELDAMQADSAMRELILSRRSMLEGWTQYRQDRAALMEEEYNYKVFDQVEHSTADEDYEVVEEIKEEILEEKEEEVTSFEEPTSSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>C5DG51</accession>
    <name>C5DG51_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0D02442p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0D02442g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552831.1">
        <property type="nucleotide sequence ID" value="XM_002552785.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DG51"/>
    <dbReference type="GeneID" id="8295051"/>
    <dbReference type="KEGG" id="lth:KLTH0D02442g"/>
    <dbReference type="HOGENOM" id="CLU_062645_2_0_1"/>
    <dbReference type="InParanoid" id="C5DG51"/>
    <dbReference type="OMA" id="NTIACGI"/>
    <dbReference type="OrthoDB" id="4061312at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005619">
        <property type="term" value="C:ascospore wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005628">
        <property type="term" value="C:prospore membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052786">
        <property type="entry name" value="Spore_wall_assembly"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34292">
        <property type="entry name" value="OUTER SPORE WALL PROTEIN LDS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34292:SF2">
        <property type="entry name" value="OUTER SPORE WALL PROTEIN LDS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="221" mass="24606" checksum="AF23A2739649CFC5" modified="2009-07-28" version="1">MIIFLIYVSKFLLFTLLYYFTLFPFVLGISTVLFGPLGITIAVVHSMLHVNLYATSVTRVGCLGHAAAILEKLFTDKGTPKENIRRLHQPAYPVVLEKRNWTQSLSIFAAKTMAHFIIFSVLLCISLIPIIGIVLAKLLRSAPIGYDYYLIHLARRKALEKRGRDEFYREIGKFTAFGIVSGALELIPILSGITITSNFLGSGICVLEQSQSFENSSLSSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>C5DG67</accession>
    <name>C5DG67_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Glutamate--tRNA ligase, mitochondrial</fullName>
            <ecNumber evidence="3">6.1.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Glutamyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0D02794g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.17" evidence="3"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552847.1">
        <property type="nucleotide sequence ID" value="XM_002552801.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DG67"/>
    <dbReference type="FunCoup" id="C5DG67">
        <property type="interactions" value="758"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DG67"/>
    <dbReference type="GeneID" id="8295067"/>
    <dbReference type="KEGG" id="lth:KLTH0D02794g"/>
    <dbReference type="eggNOG" id="KOG1149">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015768_6_3_1"/>
    <dbReference type="InParanoid" id="C5DG67"/>
    <dbReference type="OMA" id="QAPRYDN"/>
    <dbReference type="OrthoDB" id="428822at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004818">
        <property type="term" value="F:glutamate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006424">
        <property type="term" value="P:glutamyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00808">
        <property type="entry name" value="GluRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000045">
        <property type="entry name" value="Glutamate--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00022">
        <property type="entry name" value="Glu_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045462">
        <property type="entry name" value="aa-tRNA-synth_I_cd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020751">
        <property type="entry name" value="aa-tRNA-synth_I_codon-bd_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008925">
        <property type="entry name" value="aa_tRNA-synth_I_cd-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004527">
        <property type="entry name" value="Glu-tRNA-ligase_bac/mito"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000924">
        <property type="entry name" value="Glu/Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020058">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049940">
        <property type="entry name" value="GluQ/Sye"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033910">
        <property type="entry name" value="GluRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00464">
        <property type="entry name" value="gltX_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43311">
        <property type="entry name" value="GLUTAMATE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43311:SF2">
        <property type="entry name" value="GLUTAMATE--TRNA LIGASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19269">
        <property type="entry name" value="Anticodon_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00749">
        <property type="entry name" value="tRNA-synt_1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00987">
        <property type="entry name" value="TRNASYNTHGLU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48163">
        <property type="entry name" value="An anticodon-binding domain of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamyl/glutaminyl-tRNA synthetase class Ib catalytic" evidence="12">
        <location>
            <begin position="46"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class I anticodon-binding" evidence="13">
        <location>
            <begin position="408"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00072917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF19269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR22409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="546" mass="62277" checksum="BF7A7D7D1C05F71C" modified="2009-07-28" version="1">MIDGMRFILARRWNSVLSRPNRLFTSKKSILSKKPVKDIQPKTPARTRFAPSPTGLLHIGSLRTALYNYLLAKSTGGSFLLRLEDTDQNRLVPGAEKNIYESLSWCGIHYDEGPGVNDKKNGPFRQSDRTEIYQQYTNKLLDSGHAYRCFCTKERLDGLRESAQKLQPPTTASYDRHCSHLSEGEIREKLDQKVPFTIRLKSPERYPPFEDLLHGKLDMQPQVNPNDRRYDDPILVKSDALPTYHFANVVDDHLMDITHVVRGEEWLPSTPKHIALYHAFGWNPPEFIHIPLLTSVTDKKLSKRRGDADVMALRAKGVLPEALVNFSVLFGWSPPREEAAKNHECFSLPDLVRLFNLNSLTKGNAKVDEKKLWFFNKHYLTLRLQNPEVLDKMANELLGSMQSLYPSVTVSQIRRALGLVGASLNTPNELATDFPYLFSKPQYERNQYVREFIQKQDLYQIRALLQKMEGKMSTENSVLTVIERLSRDNGIRKNVVFEAARFALTGSVPGVKLPLLIEFLGAEEANLRISEALDKLDSVLCLKGEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>C5DG73</accession>
    <name>C5DG73_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0D02926p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0D02926g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FUN14 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552853.1">
        <property type="nucleotide sequence ID" value="XM_002552807.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DG73"/>
    <dbReference type="FunCoup" id="C5DG73">
        <property type="interactions" value="50"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DG73"/>
    <dbReference type="GeneID" id="8295073"/>
    <dbReference type="KEGG" id="lth:KLTH0D02926g"/>
    <dbReference type="eggNOG" id="ENOG502S4F4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103433_1_0_1"/>
    <dbReference type="InParanoid" id="C5DG73"/>
    <dbReference type="OMA" id="GWIRINL"/>
    <dbReference type="OrthoDB" id="3990500at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007014">
        <property type="entry name" value="FUN14"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04930">
        <property type="entry name" value="FUN14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR22415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="201" mass="21712" checksum="8AE6822E7ED0BB28" modified="2009-07-28" version="1">MSFARPFLFQKTALGQLRKFLSPFSFGKSRLMSSGARAAKAPLYSGFVGYKTVGFAAGTSLAMLLSTISSKNIIRSDTILDVKQRNAGLPEEIGLPGQRGKSAVSKKVNYRELCLGSVLGLMIGVVVGKISSVLVFVTACGLLSLQWLSNRGLVDKNATWGLSKYIVKTGRESVDLNTLIWDKPSFKVPFILTFVLAALNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGK3</accession>
    <name>C5DGK3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0D06028g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPL family. K-HECT subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="3"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552983.1">
        <property type="nucleotide sequence ID" value="XM_002552937.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DGK3">
        <property type="interactions" value="1299"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGK3"/>
    <dbReference type="GeneID" id="8295213"/>
    <dbReference type="KEGG" id="lth:KLTH0D06028g"/>
    <dbReference type="eggNOG" id="KOG0168">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0170">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000366_1_1_1"/>
    <dbReference type="InParanoid" id="C5DGK3"/>
    <dbReference type="OMA" id="FFTIHAQ"/>
    <dbReference type="OrthoDB" id="423283at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045322">
        <property type="entry name" value="HECTD1/TRIP12-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45670:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HECTD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45670">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="HECT" evidence="8">
        <location>
            <begin position="1097"/>
            <end position="1453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="623"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="30"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="86"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="632"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="653"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="1420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR22545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1453" mass="164165" checksum="70E3690A40A36DF7" modified="2009-07-28" version="1">MSRDEIRHSMNRDYDSDGDFVIRPSRQNDYEDEDVAEEENEYSYPEEVDDDVESYEGNMYHFGDEENERDEAYENSQDSEHSGENPTEEVDGNEGDDIEDELRNTTLRGAQRQSSLQELLGSLAQNMEGPSREHGGENSRRVNIADIFPEMFSMMGAGRSTGRGNPRLIKLLDNVTNAQDDPYIAMESLRELSEQLLMMSPLAAERAVPQEDLLKGIVDIFMDPLLQGELELQLIAFRCLYNLFEVNPDMISIAVDRNVIAACNDKLAEISYIDLAEQVLETLEIISRLHGRSVLQSGSLLACLQYLDFFTSHAQRKAVTIVVNSCAKIRAVDLGHISDIMWRLKEIFVSSQDHSLRQNMLNVFYGICAGLGRGDKTLEGLFDFELIESIMQLISNGETNLESRLKAFDILSQVLVSGEVLAPQIIGCGRVLDMLMSSMNEYKKSTASQLHETVMFAPKPLLTCISRFIVVLFPGEDKQILSADTPKQYEFQGIEEQSRQLVKGLGPFLVQVYVNAVDFQIRRLVLVALARVLSPHIPIETCDIDKGIVSMIASSLARNMALFEQSGFKDQVSGVLLLGSLRLVTSLLEKNASEHLPAFVREGVFESFKSFSKELSSIVPESLEKTQAGDPPQNNGKFTDISDTNSESNYHSDDDDYNMEFEGMESPDHTRPNRVCFRILKHLKLNYVQNELANTLESVIESYGGEGAVISEISEINSLVERLRLLDVHSDTYEYWQRIWLEVKSKLFMSNFVCSGFEFISSGLADEMSKLVLSNSSKSSICQRALIETFGEDLETFAEIIQSALTRLESFELLDCGLHGEDGKGASLGKQVKIRLEYDGDAVEDGIPKNLQAVTVAIHCVATFRSLNEFLKHRVLQSQLITSLIPALAMRVDRQDDDVKWSFDFAFNNTKCDHQDTIFGAIFRPIKNAGKDSKSLWSEPHIIRYKKAQGKDSDQKLSTLYFGGPRGLNELGRLEGLLVLLKCLRGSSLRPECFVNSKISAKLACQLEEPLIVAGGLLPSWTVLLTRNYPFLFPFETRMFFLQSSSFGHGRLIQVWRNRVGSDKGDFADALHQLGRPTRRKLKVSRGAVFLSALKILDKYGSTPNILEVEFLNEVGTGLGPTLEFFAIVSKEFAYKSLRMWRSENFNSCKEGELVEEHLFPAPLLASSNQEKTLELFHKLGTFVSRSMLDDRILDFRFSKAFFELAHMYARNEKIRFDDFDLTIDYMSMVDPHLAKSMKYLFTLKNDHDFESLSLTFYLSGYNIELIENGKDVAVTIENFPDYFERLSDQVLGTGVKMQIQSFIEGFSRTFPYTSLLALTPDELTDLFGSVEEDWTHETLYAYVDADHGYSHDSNIIHDLISIMTSFSKAERRLFLEFLTGSPKLPIGGFKSLNPRMTVVRKHTENDLEPDHYLPSVMTCANYLKLPKYSNKDVLKSRVLQAMKEGLGAFLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGN7</accession>
    <name>C5DGN7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">KLTH0D06798p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0D06798g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553017.1">
        <property type="nucleotide sequence ID" value="XM_002552971.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGN7"/>
    <dbReference type="GeneID" id="8295248"/>
    <dbReference type="KEGG" id="lth:KLTH0D06798g"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2158880_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGN7"/>
    <dbReference type="OrthoDB" id="6077919at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000125">
        <property type="entry name" value="Putative zinc finger protein 143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001228">
        <property type="entry name" value="Zinc finger protein 236"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003:SF20">
        <property type="entry name" value="FINGER DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G10380)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR PROTEIN YY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="49"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="77"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR22579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="111" mass="12279" checksum="190978D6EF05F14B" modified="2009-07-28" version="1">MQRQPASPSWAGPEHILGLPRHSSAPLTSAPQRPSAVPTPAEAPKLLRYQCSRCLKVFTRSSALQSHILVHTGDRPFVCPHSSCSKSFNVKSNMNRHFKTHKQLGLRRGST</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGP9</accession>
    <name>C5DGP9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0D07084p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0D07084g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the FNT transporter (TC 1.A.16) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553029.1">
        <property type="nucleotide sequence ID" value="XM_002552983.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGP9"/>
    <dbReference type="FunCoup" id="C5DGP9">
        <property type="interactions" value="26"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGP9"/>
    <dbReference type="GeneID" id="8295260"/>
    <dbReference type="KEGG" id="lth:KLTH0D07084g"/>
    <dbReference type="eggNOG" id="ENOG502QUGF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020549_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGP9"/>
    <dbReference type="OrthoDB" id="4829at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015513">
        <property type="term" value="F:high-affinity secondary active nitrite transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015707">
        <property type="term" value="P:nitrite transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000292">
        <property type="entry name" value="For/NO2_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024002">
        <property type="entry name" value="For/NO2_transpt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30520">
        <property type="entry name" value="FORMATE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30520:SF6">
        <property type="entry name" value="FORMATE_NITRATE FAMILY TRANSPORTER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01226">
        <property type="entry name" value="Form_Nir_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01005">
        <property type="entry name" value="FORMATE_NITRITE_TP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="91"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="120"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="161"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="194"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="238"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="296"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="531"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="302"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="344"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="362"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="414"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="555"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="607"/>
            <end position="622"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR22591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="663" mass="72497" checksum="53E41890701E59C5" modified="2009-07-28" version="1">MSEDEYYISPHETALAVVATAMKKARLTLTTLALNSLAGGVLFSTGGLLYTSWHGSNQAILENDPGVLNFLGAFTFSIGLFYVVINGTDLFNSNILFFSVAALRGAVSIYDLLISWTVSWIGNFTGTLFVVYVMCHLSGTTSSADLVSFTHQMVAKKDSSSFIQTFIKGIAGNFYVSLAIYLQLMAKPLHVKYLMMTLPIFTLVAAGFTHVVAEMFLLMVGMFNGAELSIGTFVWKHMVAVTLGNIIGGSAFALVIPYYLHLVVVEQDRKRLALPSYEARDEQPEINMDSRVVRVPTQEAEKVEEEAEENEEMTSEEKANLELASSDSSAEDRKAAPPVEYRPRLSSTSLSRTYSNLRRLNTNKSYSSVRSVKSLPHRTPTGVFPVKGMASPVRTNSGIPPQKREESQGLGRFSRPLSRRSSQTSSLRLSKTLGSSRRNSISVLQRPGGAFAHRSKSLPHIGLVGERTHFGTNDVLEEKMGTKLERALTRITSRVSREGRNSPGFGLPSTTQDIFPTSTGLGLHESDATASFSMDDHSSSSMLDGLPANGRRSRTPSIQKTSIQDLYLKPSTSDNLSGAINETSGSKSPPTQPQGKNSPSDGTSQKSTSSRSIPTIRRPSSIYVPDAVDQRNSVYNAGYKNTLLRNHMTPDNDYDEEQSIKDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGQ1</accession>
    <name>C5DGQ1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0D07128p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0D07128g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553031.1">
        <property type="nucleotide sequence ID" value="XM_002552985.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGQ1"/>
    <dbReference type="GeneID" id="8295262"/>
    <dbReference type="KEGG" id="lth:KLTH0D07128g"/>
    <dbReference type="eggNOG" id="KOG1286">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007946_12_2_1"/>
    <dbReference type="InParanoid" id="C5DGQ1"/>
    <dbReference type="OMA" id="AMFSTAN"/>
    <dbReference type="OrthoDB" id="3900342at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000001">
        <property type="entry name" value="Amino acid permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004840">
        <property type="entry name" value="Amino_acid_permease_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF3">
        <property type="entry name" value="AMINO-ACID PERMEASE PB1C11.02-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00218">
        <property type="entry name" value="AMINO_ACID_PERMEASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="48"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="159"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="274"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="325"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="371"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="400"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="442"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="475"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="9">
        <location>
            <begin position="47"/>
            <end position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR22593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="543" mass="60721" checksum="3CD31DE0F21326AB" modified="2009-07-28" version="1">MSLKNDQEVEVIGIATSQSLSSDTQDAGALNRGHVRENLNRSLTPRHINMISIAGVIGTGLYLGTGKALANGGPASLLINYGIIGLVVYLIMLCLGEMSTFMPISGSFCSYAKKFGSGSLAFTLMCNYWFNDAVSVASDLTALQLILDYWKNPNSHFPYWAASLIFWFALLLLNVVHVRVYGEAEYWLALLKVIAIIMFFIISIIVNAGHNSDHAYIGFKNWGVGDAPFVNGFKGFASLFVSSSFAFGGTESITLTAGEAVNPVRNTPKIIKTVFWRILIFYVFTAFFIGMNVPYNYPNLSEKSVMTSPFTIVFQMVGSRGAGSFMNAVILTSVISAGNHALFAGSRVLFNIGLEGYFFPSFITKTNRYQVPYVAVLITWAAGGLCFGSSFIGAGTLWTWLQNIVGVSNQLAWLCISVISIRFRRGLARQGKTHELQFVNWTYPYGPYFLVVFVTFIIFVQGWSSFAPWSVSDFFSYYIELIVFVALYAFWWLWKRDSFVKSDEMDFVTDKYVSPEEEVLLNERLDNLKGWKKVRQVVDEYFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGQ4</accession>
    <name>C5DGQ4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0D07194p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0D07194g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553034.1">
        <property type="nucleotide sequence ID" value="XM_002552988.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGQ4"/>
    <dbReference type="FunCoup" id="C5DGQ4">
        <property type="interactions" value="212"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGQ4"/>
    <dbReference type="GeneID" id="8295265"/>
    <dbReference type="KEGG" id="lth:KLTH0D07194g"/>
    <dbReference type="eggNOG" id="KOG2914">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045011_13_0_1"/>
    <dbReference type="InParanoid" id="C5DGQ4"/>
    <dbReference type="OMA" id="FHHMVMG"/>
    <dbReference type="OrthoDB" id="40579at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.240:FF:000001">
        <property type="entry name" value="Haloacid dehalogenase-like hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="entry name" value="Putative phosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18901">
        <property type="entry name" value="2-DEOXYGLUCOSE-6-PHOSPHATE PHOSPHATASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18901:SF38">
        <property type="entry name" value="PSEUDOURIDINE-5'-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR22596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="223" mass="24701" checksum="7BBC9C1C6B9E27C7" modified="2009-07-28" version="1">MDGLLINTEDIYTLVTNEILTEYGKGPLTWDLKVQLQGLPGPEACRKVLDHFQLPVTPQEFDRKNIELQCKMWPKCSFLPGALDLIRYLHSKNIPIALCTSSAKHKFEGKTSHLKHGFELFDAIVTGDDPRIPPGRGKPFPDIWLVGLKELNNKFKANIQPEECLIFEDGVPGVTAAKAAGGYVVWIPHPDAYDVLGDTDAILDGKGELISSLVHLDKAKFGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGR1</accession>
    <name>C5DGR1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0D07392p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0D07392g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553041.1">
        <property type="nucleotide sequence ID" value="XM_002552995.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGR1"/>
    <dbReference type="GeneID" id="8295273"/>
    <dbReference type="KEGG" id="lth:KLTH0D07392g"/>
    <dbReference type="eggNOG" id="ENOG502QR4F">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026445_1_0_1"/>
    <dbReference type="InParanoid" id="C5DGR1"/>
    <dbReference type="OMA" id="GPDKYHL"/>
    <dbReference type="OrthoDB" id="2153176at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012469">
        <property type="entry name" value="DUF1688"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31687">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31687:SF3">
        <property type="entry name" value="PROTEIN URG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07958">
        <property type="entry name" value="DUF1688"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR22603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="448" mass="48555" checksum="F2E698BDE51607C0" modified="2009-07-28" version="1">MTIEAVEYLKSIKSVRDTTSQVFNFAVSNGGGHYFDLDLGQMPRVADFLCEIVKRDYGTEYASIPPHGRWQHLNHGHPGRVEGLIAQWRDSGVDEVEVARKLVDLLVFSVLVDAGAGNSWKFTVPGSSTSVGRSEGLAVASYYLFVEGSLSDDRDDPYKVTGRALSNFDAELFSRGFQVSEANPLAGAPGRVSLIQRLGAALLARPDIFGAEARPGALVDYLYAKSTVTSDAAGATSPTIDLQDVWDALMQGLTSIWPAGRTVVNGEPLGDAWRLDTKAAASSDHTPAGVPSDIVTFHKLTQWLCYSLLVPLERYGYKFRISNKSLQTGLPEYRNGGLFYDFGVLKLKSDALKGGLAFSAQLRDAGHDASDTIPTFEPHQGAIVEWRCLTVGLLDSLLPLVNKKLEYELGLPQLIEAGSWKAGREIAAKLRPATGGPPIELYSDGTVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGW6</accession>
    <name>C5DGW6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0D08844p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0D08844g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553096.1">
        <property type="nucleotide sequence ID" value="XM_002553050.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGW6"/>
    <dbReference type="FunCoup" id="C5DGW6">
        <property type="interactions" value="241"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGW6"/>
    <dbReference type="GeneID" id="8295332"/>
    <dbReference type="KEGG" id="lth:KLTH0D08844g"/>
    <dbReference type="eggNOG" id="KOG1576">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_7_2_1"/>
    <dbReference type="InParanoid" id="C5DGW6"/>
    <dbReference type="OMA" id="FPRSSYK"/>
    <dbReference type="OrthoDB" id="5286008at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045290">
        <property type="term" value="F:D-arabinose 1-dehydrogenase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070485">
        <property type="term" value="P:dehydro-D-arabinono-1,4-lactone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19164">
        <property type="entry name" value="AKR_ARA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044480">
        <property type="entry name" value="Ara2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42686">
        <property type="entry name" value="GH17980P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42686:SF1">
        <property type="entry name" value="GH17980P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="2">
        <location>
            <begin position="24"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR22658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="340" mass="37922" checksum="3392665EE88DBE83" modified="2009-07-28" version="1">MDSLKPIGPTQGLVRGLGVENLPKLILGGATLNTQYNDDPASLPVVDMIRFAMQFGVRAIDTSPYYGDSEVLYGAALESLKEELPRENYFICTKVGRIRLEEFDYSRSHIHFSVLRSCERLKTKYLDVVFLHDVEFVETEQVLEALQELRKLRDEGVILNFGISGYPVDFLLNIALKCKTHPQIGALDVVLSYANLNLQNRTLHEYAPRFREEAGVKVVENGSILSMSLLRSQETKAFHPCSAELRKLSNEAAQYAASQGEDLASLATRYAFSEWADKGPTVLGVSSVAELRVALESYTIVADNQGKLSEKDARLANEIQSQIFGAHMNETWSSGIDHEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHB1</accession>
    <name>C5DHB1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0E02860p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0E02860g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the UTP23/FCF1 family. FCF1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553609.1">
        <property type="nucleotide sequence ID" value="XM_002553563.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHB1"/>
    <dbReference type="FunCoup" id="C5DHB1">
        <property type="interactions" value="991"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHB1"/>
    <dbReference type="GeneID" id="8291754"/>
    <dbReference type="KEGG" id="lth:KLTH0E02860g"/>
    <dbReference type="eggNOG" id="KOG3165">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081098_0_1_1"/>
    <dbReference type="InParanoid" id="C5DHB1"/>
    <dbReference type="OMA" id="GMMDCLL"/>
    <dbReference type="OrthoDB" id="76105at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:RNA nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09864">
        <property type="entry name" value="PIN_Fcf1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000004">
        <property type="entry name" value="rRNA-processing protein FCF1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006984">
        <property type="entry name" value="Fcf1/UTP23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037503">
        <property type="entry name" value="Fcf1_PIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12416">
        <property type="entry name" value="RRNA-PROCESSING PROTEIN UTP23 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04900">
        <property type="entry name" value="Fcf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="PIN" evidence="6">
        <location>
            <begin position="63"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR23172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="189" mass="21451" checksum="E338A8359FFC5378" modified="2009-07-28" version="1">MGKAKKTRKFALVKRTLNAKKDVRLKANQAKEAHRDDPELTKNVPQISSALFFQYNQAIKPPYQVLIDTNFINFSIQKKIDIVRGMMDTLLAKCIPMITDCVMAELEKLGPKYRIALKLARDPRIQRLTCSHKGTYADDCLVNRVLQHKCYIVATNDAGLKQRIRKVPGIPLMSVGGHAYVIEKLPDVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHD0</accession>
    <name>C5DHD0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0E03344p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0E03344g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M20A family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553628.1">
        <property type="nucleotide sequence ID" value="XM_002553582.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHD0"/>
    <dbReference type="GeneID" id="8291775"/>
    <dbReference type="KEGG" id="lth:KLTH0E03344g"/>
    <dbReference type="eggNOG" id="ENOG502QPR4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024588_2_0_1"/>
    <dbReference type="InParanoid" id="C5DHD0"/>
    <dbReference type="OMA" id="CSSGVWA"/>
    <dbReference type="OrthoDB" id="4676at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016813">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amidines"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03884">
        <property type="entry name" value="M20_bAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010158">
        <property type="entry name" value="Amidase_Cbmase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01879">
        <property type="entry name" value="hydantase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32494:SF5">
        <property type="entry name" value="ALLANTOATE AMIDOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32494">
        <property type="entry name" value="ALLANTOATE DEIMINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001235">
        <property type="entry name" value="Amidase_carbamoylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR23191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="433" mass="48344" checksum="A65D3B7CD9D1CCE2" modified="2009-07-28" version="1">MRNQSKCHSLKIKKGRLNQTIIDTGERFGGVERWGNKPHEFGMRRLAGTSADGAMRNWFVRECRSLGCDIKVDQIGNIFAIFRGREKGKPTAIGSHLDTQPQAGKYDGILGVLAGLEVLRTFKENDFVPHYDVCVIVWFNEEGARFTRYCMGSSVWAGTLDLEEAYGMLSVTDVKRESVRDSLLNIGYLGDAPASYLENGIDAHYELHIEQGPVLEDEEKKIGVVTGVQACYWEKFTVFGIGAHAGTTPWRLRKDALNVALRMIIKAEEVARKFGGLFTCGMIDVRPYSANIIPSEASFTLDYRHDFDETLFKMLEEAKASFSQLVESEEMSYKSERLAYTPVVNFNPACVKCVSKSALSLFREIAVKNMQSGAGHDSCQVSTRVPTSMIFVPSKDGLSHNYREHTSPEDVESGLEVLLGAVINHDTLRAERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHD1</accession>
    <name>C5DHD1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0E03366p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0E03366g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553629.1">
        <property type="nucleotide sequence ID" value="XM_002553583.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHD1"/>
    <dbReference type="GeneID" id="8291776"/>
    <dbReference type="KEGG" id="lth:KLTH0E03366g"/>
    <dbReference type="HOGENOM" id="CLU_1061982_0_0_1"/>
    <dbReference type="InParanoid" id="C5DHD1"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="165"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="262" mass="30538" checksum="89DED75C566DE8C8" modified="2009-07-28" version="1">MSIEFENHVISTEGQLKSRFKYLLECAYLKFFKVTTGDERNPFAFTGDDKVDNMLESLARLKNDAETRRSEIQKLEVERQRNRKNIGQAVCDYSMPIVEENLDASESESSLSAIDDVNKPNRRTIKRKRVLEPPWNLPLLNHIRNSLPDTSLMADQLNLGLTNSIAQAAEDRKRKDRELDTQQERLELDTQQERLELELQRQKMDMKRQEVDLKLQEMKLKSQEAQKLIQLLELAGKSDGVERLCPRLTFLLQEKLDSLFKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHH9</accession>
    <name>C5DHH9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0E04466p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0E04466g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the membrane magnesium transporter (TC 1.A.67) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553677.1">
        <property type="nucleotide sequence ID" value="XM_002553631.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHH9"/>
    <dbReference type="FunCoup" id="C5DHH9">
        <property type="interactions" value="70"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHH9"/>
    <dbReference type="GeneID" id="8291826"/>
    <dbReference type="KEGG" id="lth:KLTH0E04466g"/>
    <dbReference type="eggNOG" id="ENOG502S8V0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_132206_1_0_1"/>
    <dbReference type="InParanoid" id="C5DHH9"/>
    <dbReference type="OMA" id="STHYHTD"/>
    <dbReference type="OrthoDB" id="44756at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053279">
        <property type="entry name" value="EMC_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018937">
        <property type="entry name" value="MMgT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28144">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28144:SF1">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10270">
        <property type="entry name" value="MMgT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950481" evidence="7">
        <location>
            <begin position="20"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="54"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR23240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="145" mass="16363" checksum="9F04A86BC153E569" modified="2009-07-28" version="1">MPLVSKALLVLAIFQLLHSGFSSHEFLTIKKRLVTQSNLDVAHLQLPKDVQLEAMSGVILLILSVFMSFSKLCYLSLGGKLKVLRVNEYLKEINMNKATSDKNLAGCNPYGEMCYTPNLVDVHAKREQVAQWNKIRDPELFAKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHK6</accession>
    <name>C5DHK6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0E05082p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0E05082g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553704.1">
        <property type="nucleotide sequence ID" value="XM_002553658.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHK6"/>
    <dbReference type="FunCoup" id="C5DHK6">
        <property type="interactions" value="107"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHK6"/>
    <dbReference type="GeneID" id="8291854"/>
    <dbReference type="KEGG" id="lth:KLTH0E05082g"/>
    <dbReference type="eggNOG" id="ENOG502QPR6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_683473_0_0_1"/>
    <dbReference type="InParanoid" id="C5DHK6"/>
    <dbReference type="OMA" id="NGYARIM"/>
    <dbReference type="OrthoDB" id="2020070at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055100">
        <property type="entry name" value="GNAT_LYC1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053013">
        <property type="entry name" value="LAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34815">
        <property type="entry name" value="LYSINE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34815:SF2">
        <property type="entry name" value="N-ACETYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22998">
        <property type="entry name" value="GNAT_LYC1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LYC1 C-terminal" evidence="1">
        <location>
            <begin position="220"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="403" mass="45828" checksum="30A235295DCA5202" modified="2009-07-28" version="1">MTKQTQKDNLVFEETKDPEVIRFTHMQNGENWAPPFMSKEDYLQRERVIGTSEISTKHLCPKMQKRFPEGSECLGIKYYVLRNLGLPATSKTSQVVASCETLNRVGMAASPQTGGAVQPVLTACIGGVYTLPEYRCKGYGSQMITRLNKLYDEYSQRADTPPLAKTVVMTLYSEVGEYYSKFGFSSRPVPVHVVKNIDRLLNEYCGGEPGGGDGGGRALDFDGCESLLERQVREFSQNITRLQKENPNAFVFAVEPSLDNFRLFHRRGLFHKKYVEPDSPVTFGYALPNGSHLVWHHNYYPGDRALFLLKLHVSEDSEDKDADLKLLLAQAICEAKKTKIDRLQFWDTELGHQAWSKPFMEFLGKIEDKSGLYQENPSRSAVRVSGFKGEDIIWDHNTKYCWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHM3</accession>
    <name>C5DHM3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0E05522p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0E05522g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553721.1">
        <property type="nucleotide sequence ID" value="XM_002553675.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHM3"/>
    <dbReference type="STRING" id="559295.C5DHM3"/>
    <dbReference type="GeneID" id="8291874"/>
    <dbReference type="KEGG" id="lth:KLTH0E05522g"/>
    <dbReference type="eggNOG" id="ENOG502RYSQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085409_0_0_1"/>
    <dbReference type="InParanoid" id="C5DHM3"/>
    <dbReference type="OMA" id="GDHEIWV"/>
    <dbReference type="OrthoDB" id="2015405at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042602">
        <property type="term" value="F:riboflavin reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002563">
        <property type="entry name" value="Flavin_Rdtase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050268">
        <property type="entry name" value="NADH-dep_flavin_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30466">
        <property type="entry name" value="FLAVIN REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30466:SF1">
        <property type="entry name" value="FMN REDUCTASE (NADH) RUTF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01613">
        <property type="entry name" value="Flavin_Reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00903">
        <property type="entry name" value="Flavin_Reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavin reductase like" evidence="2">
        <location>
            <begin position="26"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR23284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="208" mass="23328" checksum="B9EEFC82704DD1E9" modified="2009-07-28" version="1">MRFPIVRFQPTLGRSALVQQKFKDSMARMGGQAMILTSAGKKALPHDLFRGVTLSSVSSLSLKPQPLLQFNLQLPSFTSESLHMHEYFALHLLKPDANSITLARAFSKGAMKNHENGEVVPTQPFKELTESVHYRTYGLSNSDLAVPILINSERVFVCRKKEVFRVGDHEIWVGMVDDIIEGDDVNQDVSGGLLYCNRKFHKLGGRIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHM8</accession>
    <name>C5DHM8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">60S ribosomal protein L8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0E05632g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the eukaryotic ribosomal protein eL8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553726.1">
        <property type="nucleotide sequence ID" value="XM_002553680.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHM8"/>
    <dbReference type="FunCoup" id="C5DHM8">
        <property type="interactions" value="1310"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHM8"/>
    <dbReference type="GeneID" id="8291879"/>
    <dbReference type="KEGG" id="lth:KLTH0E05632g"/>
    <dbReference type="eggNOG" id="KOG3166">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055193_0_0_1"/>
    <dbReference type="InParanoid" id="C5DHM8"/>
    <dbReference type="OMA" id="TTLCLCG"/>
    <dbReference type="OrthoDB" id="29563at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000003">
        <property type="entry name" value="60S ribosomal protein L7a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050257">
        <property type="entry name" value="eL8/uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004037">
        <property type="entry name" value="Ribosomal_eL8-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018492">
        <property type="entry name" value="Ribosomal_eL8/Nhp2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001921">
        <property type="entry name" value="Ribosomal_eL8_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23105">
        <property type="entry name" value="RIBOSOMAL PROTEIN L7AE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00881">
        <property type="entry name" value="L7ARS6FAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00882">
        <property type="entry name" value="RIBOSOMALL7A"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01082">
        <property type="entry name" value="RIBOSOMAL_L7AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="7">
        <location>
            <begin position="122"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="237"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="8"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="244"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU367042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR23289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="256" mass="28138" checksum="25BDD2E1B6FAD29E" modified="2009-07-28" version="1">MAPGKKVAPAPLASKAGKAGKAKNPLTNSTPKSFGIGQDIQPKRNLSRYVKWPEYVRLQRQKKILSIRLKVPPAIAQFQHTLDRNTASETFKLFNKYRPETAAEKKERLTKEAAAIAEGKTRQEASPKPYVVKFGLNHVVSLVENKKAKLVLIANDVDPIELVVFLPALCKKMGVPYAIVKGKARLGTLVNQKTSAVAALTEVREEDESALAKLVSTVNANYLEKYEETKKHWGGGIVGQKAQAKKDQRAKASEAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHR2</accession>
    <name>C5DHR2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9">Histone deacetylase</fullName>
            <ecNumber evidence="2 9">3.5.1.98</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0E06446g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>N(6)-acetyl-L-lysyl-[histone] + H2O = L-lysyl-[histone] + acetate</text>
            <dbReference type="Rhea" id="RHEA:58196"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="3.5.1.98"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 9">Belongs to the histone deacetylase family. HD Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.98" evidence="2 9"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553760.1">
        <property type="nucleotide sequence ID" value="XM_002553714.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHR2"/>
    <dbReference type="FunCoup" id="C5DHR2">
        <property type="interactions" value="156"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHR2"/>
    <dbReference type="GeneID" id="8291914"/>
    <dbReference type="KEGG" id="lth:KLTH0E06446g"/>
    <dbReference type="eggNOG" id="KOG1342">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007727_7_4_1"/>
    <dbReference type="InParanoid" id="C5DHR2"/>
    <dbReference type="OMA" id="CYETSIC"/>
    <dbReference type="OrthoDB" id="1918432at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070210">
        <property type="term" value="C:Rpd3L-Expanded complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034967">
        <property type="term" value="C:Set3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141221">
        <property type="term" value="F:histone deacetylase activity, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010557">
        <property type="term" value="P:positive regulation of macromolecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11598">
        <property type="entry name" value="HDAC_Hos2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.800.20:FF:000007">
        <property type="entry name" value="Histone deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003084">
        <property type="entry name" value="HDAC_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF36">
        <property type="entry name" value="HISTONE DEACETYLASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037913">
        <property type="entry name" value="His_deacetylse_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01271">
        <property type="entry name" value="HISDACETLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="14">
        <location>
            <begin position="45"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="409"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="159"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="322"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR037913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR23323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="449" mass="51111" checksum="8A97BB2C99E0A74C" modified="2009-07-28" version="1">MSDTLQFDAKTESLKPRFEFGAPYEPNVSYHFNSDVSQFHYGVRHPMKPFRLMLTDHLVSSYGLHKIMDLYETRPATREELCEFHADDYVEFLAKVSPDNLSKLPRGTLEKFNIGDDCPIFQGLYQYSTLYAGASLDATRKLINGQSEIAINWSGGLHHAKKSSPSGFCYVNDIVLSVLNLLRYHPRVLYIDIDLHHGDGVQEAFYTTDRVFTVSFHKYNGEFFPGTGNLDETGCSRGKHFALNVPLEDGIDDDSYINLFKSIIDPLITSFKPTVIIQQCGADSLGHDRLGCFNLNVKAHGECVRFVKSFGIPMLVLGGGGYTPRNVSRLWTYETALLNGVSVPQEIPEDIPFRDWFGPDYTLHPTLDDLYENKNSKKFLENVRIRCLENIRFLQGAPSVRMDQEVIPSQDLSALTQEEEDAIKELNEETDRLRLEQMEKDSMRIGELY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHS0</accession>
    <name>C5DHS0_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cystathionine beta-lyase</fullName>
            <ecNumber evidence="3">4.4.1.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Cysteine-S-conjugate beta-lyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0E06644g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L,L-cystathionine + H2O = L-homocysteine + pyruvate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:13965"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:58161"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>an S-substituted L-cysteine + H2O = a thiol + pyruvate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:18121"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29256"/>
            <dbReference type="ChEBI" id="CHEBI:58717"/>
            <dbReference type="EC" id="4.4.1.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="8">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homocysteine from L-cystathionine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the trans-sulfuration enzymes family.</text>
    </comment>
    <dbReference type="EC" id="4.4.1.13" evidence="3"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553768.1">
        <property type="nucleotide sequence ID" value="XM_002553722.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHS0"/>
    <dbReference type="FunCoup" id="C5DHS0">
        <property type="interactions" value="176"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHS0"/>
    <dbReference type="GeneID" id="8291923"/>
    <dbReference type="KEGG" id="lth:KLTH0E06644g"/>
    <dbReference type="eggNOG" id="KOG0053">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018986_2_1_1"/>
    <dbReference type="InParanoid" id="C5DHS0"/>
    <dbReference type="OMA" id="NCIGATG"/>
    <dbReference type="OrthoDB" id="2545919at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047804">
        <property type="term" value="F:cysteine-S-conjugate beta-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071266">
        <property type="term" value="P:'de novo' L-methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000013">
        <property type="entry name" value="Cystathionine beta-lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000046">
        <property type="entry name" value="Cystathionine gamma-lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006238">
        <property type="entry name" value="Cys_b_lyase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054542">
        <property type="entry name" value="Cys_met_metab_PP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01329">
        <property type="entry name" value="cysta_beta_ly_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808:SF50">
        <property type="entry name" value="CYSTATHIONINE BETA-LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808">
        <property type="entry name" value="TRANS-SULFURATION ENZYME FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001434">
        <property type="entry name" value="CGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00868">
        <property type="entry name" value="CYS_MET_METAB_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="5 13 14" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="13">
        <location>
            <position position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00072331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR001434-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU362118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR23331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="460" mass="51017" checksum="D6CC05B1BAF024E5" modified="2009-07-28" version="1">MSQQSLQTKLVSVVQHVDQHGSSVPPIYQSTTFKIGSLEDDASQPYDYTRSGNPTRTVVQHQIGKLYDVDAAQVLAVSSGMTALDVILRALVLTNKSRVPTIIAGDDLYGGTQRLLTHLESRNHARVLHVDTANYETFVEVFKQQEHVDCVLLESPTNPLLKVADLPRLTHFIKSANEACLVAIDNTMMSGMLCNPLKFQCDVVYESATKYLNGHHDIMAGIVVTQTLKMAQEIYFVINSTGSGLSPFDSWLLVRGLKTLGVRLYQQQHNAMVLAHWLENSCGFVGKKGVKGSPRTRFVGLKSHPQFELHKSFNAGPGAVLSFETGSYEHSRRLVSSRKIKIWAVTVSFGCVNSLISMPCKMSHAAIDPALRQERDFPEDLVRLCCGIENISDLQNDLLEAMIDADIIELRDEGKTIYNKLNGHIATNTIGQGKLPNIYEEFFSQDLVERGSILTSRAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>C5DI75</accession>
    <name>C5DI75_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0E10296p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0E10296g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553923.1">
        <property type="nucleotide sequence ID" value="XM_002553877.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DI75">
        <property type="interactions" value="112"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DI75"/>
    <dbReference type="GeneID" id="8292088"/>
    <dbReference type="KEGG" id="lth:KLTH0E10296g"/>
    <dbReference type="HOGENOM" id="CLU_246834_0_0_1"/>
    <dbReference type="InParanoid" id="C5DI75"/>
    <dbReference type="OrthoDB" id="4049601at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="CDD" id="cd13746">
        <property type="entry name" value="Sir4p-SID_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.730">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031556">
        <property type="entry name" value="SIR4_SID"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16991">
        <property type="entry name" value="SIR4_SID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sir4 SID" evidence="3">
        <location>
            <begin position="885"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="108"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="170"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="341"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="443"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="475"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="533"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="615"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="719"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="803"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1272"/>
            <end position="1302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1351"/>
            <end position="1380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1459"/>
            <end position="1480"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1239"/>
            <end position="1270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="185"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="226"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="255"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="378"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="479"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="534"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="558"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="615"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="803"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1273"/>
            <end position="1288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1351"/>
            <end position="1360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1361"/>
            <end position="1374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR23486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1540" mass="166939" checksum="F914425E657E17DA" modified="2009-07-28" version="1">MSKGDVSPKTGTPLSHHSQELPHPIDASKITLYKSTPTRRVNDEKNSIGGPTLSPSASAGAYYGSNRLLSLLRIKNERTEGSAHSSDAAANEYPSTSAASSLTKIIVEPTPGSKDDNAQSEGQLSNGFEKRSFHSQVSNWISNEDAKLDIYPKRASQKIDIPLLSCNFSRTTPSTPSEKAKSGLNLLSTPLSGRTSAPKHAPSADPVPHSGQSSEPASNVVLIPPQKSSSQTSVVQKSSSAPTIPAAEQSLIKAKTTTSAQTEPSQSHPERLLPSSALHKECSSRLTGPHSLIPGTVVVAAPNEGNSVSSNRASNAPSHNAILNFGELPEDAVRAAAMATPVKDGSNHDTQGVISAPSDAPLVLERQNTVSKSDRNLRPTTLNPPSRASSRQTGRASESVSPNPRRRGKSFSAPKVPVIVQPISSSLTKAHLNGSIFTNGDKGAVLTHSPSQTKGPASHDAVEQNNMKVVLPVSREASHSQTPFSSFSKADDARQKSNANLSFNNSQNAVPVQERLTLVGRNKEDLVRSPVDTRASNPQAQVFIGTSDTTGGSLPEIRSNELDEHHDVINSSKRKRSPQDSQFEMHKKRKIDYEDASPILEASSNVNETFSNVTHTAASSVSGQKNENATVSPHSDPSSGAANNNYLKTSTKQPLSRSLESVARERKSVGVASRGYKTSASNKRINGSAALKSSDPAIVRLEVPVLVSSEGKVRGRRALAERDRGSVRKVPQSATGGEKALASEMKSPRRLRKSAKNISNGKLRSLDNVRHTRTGAKIINPLKPLPEAVVSAQDPNEVEKKANHEVLPDTQPTKDQKLENAPLLRSDDAQQVTSDKLSSEISDYLSDSFDPEGELLLAKRASNAKLHAEKNKPQETAAASQILPYIANEAKPIHDPEACRKEIEKIVSRQVARKMASARKLGKQLIVSLTQSGIVTHTPFGSGQNVTASNNICNINNYEVSPVLYLQHLSKRGRLEVSEFSQSKIPIMKVTQSSIDASTKEKLKKACNGLLVFIDNYHYGSLTENQKVLVDQRLAFVKSALPKLGMRLTDDMSSQVSIMIAVSEVQNMEDVNSVEATEAYGMQAWSYGYAVEFFKMLLGPEALPSPVLKDKEEGASDNHAQQKTISKVASACTPSNEGDGKACEKEEVPYHENALRELVGELANCVRSTTIDRAVLENCVREISSCVDFVVEEQLIGARLAEQFEAQNRLILAMSDELIQNQIKIASLHGRASSSEKDVDALNLKLSEERSKAENQLSELKSKLEESVREKQQVVPQQAVPQPVVPKQVGEEPNRNVNQGSSPDIHVEEIQRAGVLDHRVVNDIGLIKNALNKRADRPILANSGQEALTSSLTPRRLLSSDSVQDSSPRNNLSSAMLLPNPNVTGSSISTGWQGGLMRVLAALNNQQEAQHVAFRSENVLLREERDALRATLTGFDSILRGQQQRLSQLEEALLRERSKKRSPFDARQMAAGSTRTSEIEEERNALKSRLVGYDNIMRDQQVKLSLLETALQREKLTRKALQARLQTFAHGLVSEDEAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>C5DID2</accession>
    <name>C5DID2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0E11594p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0E11594g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553980.1">
        <property type="nucleotide sequence ID" value="XM_002553934.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DID2"/>
    <dbReference type="FunCoup" id="C5DID2">
        <property type="interactions" value="591"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DID2"/>
    <dbReference type="GeneID" id="8292147"/>
    <dbReference type="KEGG" id="lth:KLTH0E11594g"/>
    <dbReference type="eggNOG" id="ENOG502S5RS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_642614_0_0_1"/>
    <dbReference type="InParanoid" id="C5DID2"/>
    <dbReference type="OMA" id="RSSWHEA"/>
    <dbReference type="OrthoDB" id="4041625at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="303"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="81"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="319"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="427" mass="46630" checksum="8B9E912C14BADE96" modified="2009-07-28" version="1">MSQSTGEGENKSREGRDRLRELGLQCVSPGFGKMDNRMLSTLQVSKDIAKEQKEAIQKLSGDAKSSSPEPKINTGPDTAPSRVSGQDSGSSSTGDIEKNVAKAGPKSLKRARIPPPLNIRASGSDSGSRSGKEDNFQFTGARSAPAHITRYPRGKSRVQYLGRSTDGEHSARKKARALPNWAHMNNAAMTPYAYYPPPATAIPYQGGYPAWQGALQNPYQSPYIPAYMAPVPYAMLQAPFAQSVPDPQTAKNPASNLQRDFEAYRNKNPGVGTRDLFGNNKSRWAPIQAQPQSAREEFFGARARTPIRQSSAAGASRPRPVEKTKDKQADSGPARNNSSKEDDKDNNDDNDTEEVDLAIEEGAQPIPMHARQNSAASVANATMQGEIRLQQDSFAFTFSMLDSATDKKMFMSICDKVWDEAQEITKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-04-02" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIE3</accession>
    <name>C5DIE3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0E11836p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0E11836g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553991.1">
        <property type="nucleotide sequence ID" value="XM_002553945.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIE3"/>
    <dbReference type="STRING" id="559295.C5DIE3"/>
    <dbReference type="GeneID" id="8292158"/>
    <dbReference type="KEGG" id="lth:KLTH0E11836g"/>
    <dbReference type="eggNOG" id="KOG1039">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_746113_0_0_1"/>
    <dbReference type="InParanoid" id="C5DIE3"/>
    <dbReference type="OMA" id="DASHHEM"/>
    <dbReference type="OrthoDB" id="411372at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045072">
        <property type="entry name" value="MKRN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11224:SF10">
        <property type="entry name" value="IP09428P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11224">
        <property type="entry name" value="MAKORIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="106"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="136"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="106"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="136"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="292"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="20"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="33"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="43"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="175"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR23554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="371" mass="38632" checksum="9443FB9BC9160992" modified="2009-07-28" version="1">MPPEKPFGTPADRPAGKPPGKSRARRGPRRRGERGERGERSGSRPSSASSTSPSSASSAAPQSPVSSASQGPTSPADDKSAGFSPSQQRAIFYSRQVTKHSAQHKNYSHVPCRFFRQGACQAGDSCPFSHSLNALAADQTPCKYFQRGHCRFGAKCANAHILPDGTRANPTKYRGAPASAPASAPASLPLGEPLGGPLGAQLRGAALPDAVFSRNNAYCAGAFSSSAPGLAPAAPLQPYFTDASAVVFEDDADPAGPADSACDFDGEDFIPGELADLLTPRELERRRSSLPHRLSFNDSGPGPAAPLPAPLSASGHSPNYLASPVFDYVHQRDAAWPADPPSLASVHELGAHLARIKIPENSDSRAAGISL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIR0</accession>
    <name>C5DIR0_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">ADP/ATP translocase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">ADP,ATP carrier protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">KLTH0E14476g</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">ADP:ATP antiporter that mediates import of ADP into the mitochondrial matrix for ATP synthesis, and export of ATP out to fuel the cell. Cycles between the cytoplasmic-open state (c-state) and the matrix-open state (m-state): operates by the alternating access mechanism with a single substrate-binding site intermittently exposed to either the cytosolic (c-state) or matrix (m-state) side of the inner mitochondrial membrane.</text>
    </comment>
    <comment type="function">
        <text evidence="16">Catalyzes the exchange of ADP and ATP across the membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ADP(in) + ATP(out) = ADP(out) + ATP(in)</text>
            <dbReference type="Rhea" id="RHEA:34999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:35000"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="3 16">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554108.1">
        <property type="nucleotide sequence ID" value="XM_002554062.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIR0"/>
    <dbReference type="FunCoup" id="C5DIR0">
        <property type="interactions" value="1082"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIR0"/>
    <dbReference type="GeneID" id="8292278"/>
    <dbReference type="KEGG" id="lth:KLTH0E14476g"/>
    <dbReference type="eggNOG" id="KOG0749">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015166_12_0_1"/>
    <dbReference type="InParanoid" id="C5DIR0"/>
    <dbReference type="OMA" id="CWATIYK"/>
    <dbReference type="OrthoDB" id="270584at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005471">
        <property type="term" value="F:ATP:ADP antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009061">
        <property type="term" value="P:anaerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015886">
        <property type="term" value="P:heme transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140021">
        <property type="term" value="P:mitochondrial ADP transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990544">
        <property type="term" value="P:mitochondrial ATP transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000001">
        <property type="entry name" value="ADP,ATP carrier protein, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002113">
        <property type="entry name" value="ADT_euk_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45635">
        <property type="entry name" value="ADP,ATP CARRIER PROTEIN 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45635:SF14">
        <property type="entry name" value="ADP_ATP TRANSLOCASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00927">
        <property type="entry name" value="ADPTRNSLCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0050">Antiport</keyword>
    <keyword evidence="11 14 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 14 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 15" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="175"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="214"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="14">
        <location>
            <begin position="8"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="14">
        <location>
            <begin position="112"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="14">
        <location>
            <begin position="212"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU368008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAR23671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="304" mass="32870" checksum="DBEEFA4D4EE0BE52" modified="2009-07-28" version="1">MAETKKQSNFLVDFLMGGVSAAVSKTAAAPIERVKLLIQNQDEMIKQGSLDRRYTGIGECFKRTAANEGIASFWRGNTANVIRYFPTQALNFAFKDKIKSMFGFKKEEGYGKWFAGNLASGGAAGGLSLMFVYSLDYARTRLAADSKGAKKGGERQFNGLVDVYKKTLASDGIAGLYRGFLPSVVGIVVYRGLYFGGYDSLKPLLLTGSLEGSFLASFLLGWAVTTGASTASYPLDTVRRRMMMTSGQAVKYNGAFDAFRKIVAAEGIKSLFKGCGANILRGVAGAGVISLYDQLQMIMFGKKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJ05</accession>
    <name>C5DJ05_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0E16610p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0E16610g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sulfatase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554203.1">
        <property type="nucleotide sequence ID" value="XM_002554157.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJ05"/>
    <dbReference type="STRING" id="559295.C5DJ05"/>
    <dbReference type="GeneID" id="8292375"/>
    <dbReference type="KEGG" id="lth:KLTH0E16610g"/>
    <dbReference type="eggNOG" id="KOG3867">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006332_11_1_1"/>
    <dbReference type="InParanoid" id="C5DJ05"/>
    <dbReference type="OMA" id="YSDQWAQ"/>
    <dbReference type="OrthoDB" id="103349at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004065">
        <property type="term" value="F:arylsulfatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019637">
        <property type="term" value="P:organophosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006796">
        <property type="term" value="P:phosphate-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16025">
        <property type="entry name" value="PAS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050738">
        <property type="entry name" value="Sulfatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000917">
        <property type="entry name" value="Sulfatase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42693:SF33">
        <property type="entry name" value="ARYLSULFATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42693">
        <property type="entry name" value="ARYLSULFATASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00884">
        <property type="entry name" value="Sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sulfatase N-terminal" evidence="2">
        <location>
            <begin position="6"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR23766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="564" mass="64132" checksum="F8978EDC98684660" modified="2009-07-28" version="1">MPSRKPNFLVIVADDLGFTDIGCFGGEIETPNLDRLGRDGLRFAGFHTASACSPTRSMLLSGTDNHLAGLGQMAEYARGFPEKFEGKPGYEGYLNFRVAALSEILTPEYYTLISGKWHLGLDEPYWPDKRGFEKSFTLLPGAGNHYKFETSEKHFMPFLYQENGRRVNVDKELPEDFYSTKYFTDKFLEYLDDKEQRKDRPFLGFLTYTAPHWPLQAPQNTIKKYRGVYSKGPLALRNDRLRHAEREGIIEKGVVPHLVETKQDKVWDELDDEAKEYASRVMETYAAMVDELDKNVGRVLKRLEETSELENTVVLFMSDNGAEGMLLEALPFNGKLFGDKISSKYNNRFDNIGNKDSFVYYGDLWAQAATAPRHMYKMWATEGGINCPLILNYPPLNKQQKKEGVVSEFTTVMDILPTILDLAKIQHPGHIFQGRNVHIPRGKTWMPYLEKASDYVHDENTVTGWELFGQRAIRKGSFKAVYIPEPLGTGKWQLFDLSKDLGETTDVSTGFPEKLSDLVGYWSVYVAETGLVEINEYPEGTEYCRTIVEGNSSQEIESVTQSAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJ76</accession>
    <name>C5DJ76_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0F14124p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0F14124g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554802.1">
        <property type="nucleotide sequence ID" value="XM_002554756.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJ76"/>
    <dbReference type="FunCoup" id="C5DJ76">
        <property type="interactions" value="675"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJ76"/>
    <dbReference type="GeneID" id="8293028"/>
    <dbReference type="KEGG" id="lth:KLTH0F14124g"/>
    <dbReference type="eggNOG" id="KOG1073">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019221_2_1_1"/>
    <dbReference type="InParanoid" id="C5DJ76"/>
    <dbReference type="OMA" id="WYPPPGH"/>
    <dbReference type="OrthoDB" id="21539at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033962">
        <property type="term" value="P:P-body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034063">
        <property type="term" value="P:stress granule assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01736">
        <property type="entry name" value="LSm14_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025762">
        <property type="entry name" value="DFDF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019050">
        <property type="entry name" value="FDF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025761">
        <property type="entry name" value="FFD_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025609">
        <property type="entry name" value="Lsm14-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025768">
        <property type="entry name" value="TFG_box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13586">
        <property type="entry name" value="SCD6 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13586:SF0">
        <property type="entry name" value="TRAILER HITCH, ISOFORM H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12701">
        <property type="entry name" value="LSM14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01199">
        <property type="entry name" value="FDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01271">
        <property type="entry name" value="LSM14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51512">
        <property type="entry name" value="DFDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51513">
        <property type="entry name" value="FFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51536">
        <property type="entry name" value="TFG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sm" evidence="7">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="DFDF" evidence="4">
        <location>
            <begin position="200"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="FFD box profile" evidence="5">
        <location>
            <begin position="243"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="TFG box profile" evidence="6">
        <location>
            <begin position="262"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="114"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="151"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="FFD box" evidence="1">
        <location>
            <begin position="243"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="TFG box" evidence="2">
        <location>
            <begin position="262"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="151"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="182"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="248"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="274"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="286"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="299"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="311"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR24365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="328" mass="36646" checksum="5790873C68AC24A8" modified="2009-07-28" version="1">MAQYIGKTISLISVTDNRYVGLLEGIDSDKGVVTLNRVRCFGTEGRKQWGPQEIYPNPTIYETVTFNGNDVKDLSILDVSLEEVQPVLPPPQLVMRQQQQQQQQQQQQQQQQQQQQQQQQQSPSQSQPQIQQPQVPAAMAGYGVYAPSAPAAAAAQAPKDAPAPQQSPQQQPVPEQQPNRSRTREAPREPRAVKQQPPKPEPRKVEIPTEDFDFQSNNSKLERGHDSTGSEAEHQEKPESDETFYNKTSSFFDTISTSTETNTNMRWQEERQLNMDTFGQASVGNRRGRGGFRGRGGRGGRGGRGGRGGSRRNDGFSRSGPTKERIEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJ80</accession>
    <name>C5DJ80_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0F14212p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0F14212g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Transcription factor involved in RNA polymerase II transcription regulation. May function in both SPT15/TBP post-recruitment and recruitment steps of transcription.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IWS1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554806.1">
        <property type="nucleotide sequence ID" value="XM_002554760.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJ80"/>
    <dbReference type="FunCoup" id="C5DJ80">
        <property type="interactions" value="399"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJ80"/>
    <dbReference type="GeneID" id="8293032"/>
    <dbReference type="KEGG" id="lth:KLTH0F14212g"/>
    <dbReference type="eggNOG" id="KOG1793">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045275_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJ80"/>
    <dbReference type="OMA" id="TDYKFAP"/>
    <dbReference type="OrthoDB" id="21124at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.10">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051037">
        <property type="entry name" value="RNAPII_TF_IWS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035441">
        <property type="entry name" value="TFIIS/LEDGF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017923">
        <property type="entry name" value="TFIIS_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46010">
        <property type="entry name" value="PROTEIN IWS1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46010:SF1">
        <property type="entry name" value="PROTEIN IWS1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08711">
        <property type="entry name" value="Med26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47676">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51319">
        <property type="entry name" value="TFIIS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TFIIS N-terminal" evidence="5">
        <location>
            <begin position="177"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="279"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="328"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="355"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00037349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00037992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR24369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="367" mass="41912" checksum="FAA9D263DC832FED" modified="2009-07-28" version="1">MSSEEPEYSVGLTESHEFDSRDGASEDFGTAQTERQRKRIDMGDSDEEDTVSTEQRLESQRIINAGNDRDLELGLDQSTRRRQELEDRLDRLLKKPKSRRSRRDEDDLEQYLDERILRLKDEMNVAAQRDIETLNRRLEGEDESAVAMEKVKLLPKVVSTLSKAHLADTILDNNLLQSVRIWLEPLPDGSLPSFEIQKSLFAALETLPIKTEHLKESGLGKVVIFYTKSKRVEPKLARLADRMVAEWTRPIIGASDNYRDKRVMKLDFDVEQHRKKAVLDSAKSKKSKKRRTEDDVSGKSAYELAAARRNRAAAPSQTTTDYKYAPVSNLNSMPSNLRSSGVGSSLNNSETFKRLNSRLAKRTKRTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJB3</accession>
    <name>C5DJB3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0F15026p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0F15026g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554839.1">
        <property type="nucleotide sequence ID" value="XM_002554793.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJB3"/>
    <dbReference type="FunCoup" id="C5DJB3">
        <property type="interactions" value="108"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJB3"/>
    <dbReference type="GeneID" id="8293068"/>
    <dbReference type="KEGG" id="lth:KLTH0F15026g"/>
    <dbReference type="eggNOG" id="KOG1432">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033929_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJB3"/>
    <dbReference type="OMA" id="HEHGNDC"/>
    <dbReference type="OrthoDB" id="783096at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07383">
        <property type="entry name" value="MPP_Dcr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32440:SF0">
        <property type="entry name" value="PHOSPHATASE DCR2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32440">
        <property type="entry name" value="PHOSPHATASE DCR2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="1">
        <location>
            <begin position="273"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="574" mass="65941" checksum="8AB6BF8867E2BEF4" modified="2009-07-28" version="1">MAPSSLKRRRMLHNAVRKILIPVFILFCLATCIPVKTHFANIFQPPTKLEGYTGGLIDDVRFLKCFRWYKDCDVILEKRGRNGEFVPWMRVRKELDEEKLYSVDSGLFYRSYLYVHPCDGNKDCVSDLAIAKDRSSIPMQVLQDINAKIQSKDSSMFHGHEHHKTFWTRIFGGRRERLSLQGEDWHYRRHGIWCKYSSSNKIDEGQSITNLQIFLGSGFVDPRPMWKTAIHEMDHRNSESPTPISISIQKYDKSMDSRFVTENGILNADDGKFKILQVSDLHVSNAIERCESHLCTNEWKTQRFISRIIELETPDLVVFTGDFINGPETTDYQTAILKATECVISAKIPWAMTWGTLDYSKYASERDIFNFIKSLPYNLNYLHAKQVQDASLITTTFALQLKRDNEIFGVVYILDSTNPENAVDFLKTAYENASKNSDRDTLYSLAFQHAPIPEYRPSGSFPIIGSYNDKSPLDVPQVGIRKALDDFKIHAFSCGQEHGNDCCLQSSDSNSNKDVWLCYGGHAGVVGRYSPEDSASSVRLFRIDDSLKEITTWKRNSREPDSVYDYQFLFKDNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJH8</accession>
    <name>C5DJH8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0F16544p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0F16544g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Bud neck</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554904.1">
        <property type="nucleotide sequence ID" value="XM_002554858.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJH8"/>
    <dbReference type="FunCoup" id="C5DJH8">
        <property type="interactions" value="187"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJH8"/>
    <dbReference type="GeneID" id="8293137"/>
    <dbReference type="KEGG" id="lth:KLTH0F16544g"/>
    <dbReference type="eggNOG" id="KOG2655">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017718_8_0_1"/>
    <dbReference type="InParanoid" id="C5DJH8"/>
    <dbReference type="OMA" id="EASHAEI"/>
    <dbReference type="OrthoDB" id="416553at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031105">
        <property type="term" value="C:septin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01850">
        <property type="entry name" value="CDC_Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000238">
        <property type="entry name" value="Cell division control 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016491">
        <property type="entry name" value="Septin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006698">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="6">
        <location>
            <begin position="24"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="338"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR24467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="385" mass="44322" checksum="A5B36309722A5946" modified="2009-07-28" version="1">MSSETTLVGVSNLPNQRYKIVSNSGGTFTLMVCGESGLGKTTFINTLFQTTLKHADPQQRRHQPIKRTVEIDVLRAVLEEKNFSLRVNVIDTPGFGDNVNNNKSWQTIVDFIDEQHDSYMRQEQQPHRDVKFDLRVHAVLYFIRPTGHGLKPLDIETMKRISSRANLIPVIAKSDTLTAKELQAFKLRIRQVIEAQEIRIFTPPLDESETPDPAVMEHARQLIESMPFAIIGSETKFDNGQGSVVPARKYPWGLVEVENDAHCDFRKLRSLLLRTNLLDLVLSTEELHYEAYRRLRLEGSGASEDPSLPTRAPARKLSHNPQFKEEENALKKYFTDQVKAEEQRFRQWEQNIVNERIRLNGDLEEVQAKVKTMEEQVRSLQLRKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJJ4</accession>
    <name>C5DJJ4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">hydroxyacylglutathione hydrolase</fullName>
            <ecNumber evidence="5">3.1.2.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Glyoxalase II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0F16896g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Thiolesterase that catalyzes the hydrolysis of S-D-lactoylglutathione to form glutathione and D-lactic acid. Involved in the metabolism of methylglyoxal, a toxic compound for yeast proliferation, by converting methylglyoxal to lactate via S-D-lactoylglutathione by sequential enzyme reactions catalyzed by glyoxalase I and glyoxalase II.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(R)-S-lactoylglutathione + H2O = (R)-lactate + glutathione + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25245"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16004"/>
            <dbReference type="ChEBI" id="CHEBI:57474"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="EC" id="3.1.2.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>an S-(2-hydroxyacyl)glutathione + H2O = a 2-hydroxy carboxylate + glutathione + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21864"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58896"/>
            <dbReference type="ChEBI" id="CHEBI:71261"/>
            <dbReference type="EC" id="3.1.2.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.6" evidence="5"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554920.1">
        <property type="nucleotide sequence ID" value="XM_002554874.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJJ4"/>
    <dbReference type="FunCoup" id="C5DJJ4">
        <property type="interactions" value="438"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJJ4"/>
    <dbReference type="GeneID" id="8293153"/>
    <dbReference type="KEGG" id="lth:KLTH0F16896g"/>
    <dbReference type="eggNOG" id="KOG0813">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030571_4_0_1"/>
    <dbReference type="InParanoid" id="C5DJJ4"/>
    <dbReference type="OMA" id="CKERARF"/>
    <dbReference type="OrthoDB" id="515692at2759"/>
    <dbReference type="UniPathway" id="UPA00619">
        <property type="reaction ID" value="UER00676"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004416">
        <property type="term" value="F:hydroxyacylglutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019243">
        <property type="term" value="P:methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07723">
        <property type="entry name" value="hydroxyacylglutathione_hydrolase_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.15.10:FF:000045">
        <property type="entry name" value="Hydroxyacylglutathione hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035680">
        <property type="entry name" value="Clx_II_MBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032282">
        <property type="entry name" value="HAGH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11935">
        <property type="entry name" value="BETA LACTAMASE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11935:SF94">
        <property type="entry name" value="TENZING NORGAY, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16123">
        <property type="entry name" value="HAGH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="12">
        <location>
            <begin position="25"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00054750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR24483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="284" mass="31868" checksum="9A392BFA95041A47" modified="2009-07-28" version="1">MLRQIYQTRNMHVKVIKMRWMTGGVNYSYLLSTQDKTKSYLIDPAEPLEVIPALKTEEANTIEAVVNTHHHYDHSGGNGAMVAELHEIGVPQVQVICGSHSSPGATDIPKHLQEYKLGDLKVKCIRTPCHTQDSVCYYVADPQTGEKAIFTGDTLFTAGCGRFFEGTGEEMDVALNRRILDGVGEPNWDTTKVYPGHEYTKSNVKFVRQAVFKNRGENSSLDKLEQYCQKNEVTAGAFTLRDELAYNPFMRLDDPTVRDAVGDVKGSLSRAQVMEELRKMKNSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-04-02" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJK9</accession>
    <name>C5DJK9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0F17270p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0F17270g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554935.1">
        <property type="nucleotide sequence ID" value="XM_002554889.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJK9"/>
    <dbReference type="STRING" id="559295.C5DJK9"/>
    <dbReference type="GeneID" id="8293169"/>
    <dbReference type="KEGG" id="lth:KLTH0F17270g"/>
    <dbReference type="eggNOG" id="ENOG502S4H4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010412_4_3_1"/>
    <dbReference type="InParanoid" id="C5DJK9"/>
    <dbReference type="OMA" id="LMENHSC"/>
    <dbReference type="OrthoDB" id="428577at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1110.10:FF:000008">
        <property type="entry name" value="YOR052C-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1110.10">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035896">
        <property type="entry name" value="AN1-like_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000058">
        <property type="entry name" value="Znf_AN1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01428">
        <property type="entry name" value="zf-AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00154">
        <property type="entry name" value="ZnF_AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118310">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51039">
        <property type="entry name" value="ZF_AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="AN1-type" evidence="6">
        <location>
            <begin position="43"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR24498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="114" mass="12383" checksum="AC7B95E921786AF3" modified="2009-07-28" version="1">MTETTQSNTGDKATEVNVGNGSNGTAVLVASASRVSKKTGSKKKRKDKCFADKCGSSAVKFVGDCQFCQGHFCSRHRLMENHSCQGLKSCKEQMHQRNAEKLAAEQTIVSKIQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJM4</accession>
    <name>C5DJM4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0F17622p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0F17622g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554950.1">
        <property type="nucleotide sequence ID" value="XM_002554904.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJM4"/>
    <dbReference type="FunCoup" id="C5DJM4">
        <property type="interactions" value="107"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJM4"/>
    <dbReference type="GeneID" id="8293185"/>
    <dbReference type="KEGG" id="lth:KLTH0F17622g"/>
    <dbReference type="eggNOG" id="KOG2903">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037263_0_1_1"/>
    <dbReference type="InParanoid" id="C5DJM4"/>
    <dbReference type="OMA" id="WANSEDG"/>
    <dbReference type="OrthoDB" id="2309723at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03190">
        <property type="entry name" value="GST_C_Omega_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000162">
        <property type="entry name" value="Glutathione S-transferase Gst3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047047">
        <property type="entry name" value="GST_Omega-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016639">
        <property type="entry name" value="GST_Omega/GSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32419:SF6">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE OMEGA-LIKE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32419">
        <property type="entry name" value="GLUTATHIONYL-HYDROQUINONE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13410">
        <property type="entry name" value="GST_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13409">
        <property type="entry name" value="GST_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015753">
        <property type="entry name" value="GST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="4">
        <location>
            <begin position="47"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="48"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="218"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>glutathione</name>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="149"/>
            <end position="152"/>
        </location>
        <ligand>
            <name>glutathione</name>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="167"/>
            <end position="168"/>
        </location>
        <ligand>
            <name>glutathione</name>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Cys" evidence="3">
        <location>
            <position position="292"/>
        </location>
    </feature>
    <feature type="site" description="Lowers pKa of active site Cys" evidence="3">
        <location>
            <position position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR015753-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR015753-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR015753-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR24513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="361" mass="41122" checksum="2B39D8F4A2277A10" modified="2009-07-28" version="1">MPDAKKWANSEDGSFKRQVSSFRETISASHPVFKPGKGRYWLYVSLACPWAHRTLITRVLKGLTSVIGVSVVHWHMDEKGWRFLPAGDGSKDKCFEPSAGIACTPESQSTAGVTSESTRAGVDGTPDHNYGFARLSDLYYKAKPDYQARFTVPILWDLETETIVNNESSEIIRILNSGVFDEFADPQTNPLDLVPLKLESQIEEINSWVYDHINNGVYKSGFAESQEVYEREVTGVFEHLDRLEHILSVNYKAAVTKYGEKDAASRLYLINEQLTEADLRLYPTIVRFDPVYVQHFKCNLSTIRAGYKMIDLWLRNMYWNHSAFRSTTSFDHIKLHYTRSHPRINPLAITPLGPKPDILPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJT4</accession>
    <name>C5DJT4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0F18964p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0F18964g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0220 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555010.1">
        <property type="nucleotide sequence ID" value="XM_002554964.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJT4"/>
    <dbReference type="FunCoup" id="C5DJT4">
        <property type="interactions" value="215"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJT4"/>
    <dbReference type="GeneID" id="8293246"/>
    <dbReference type="KEGG" id="lth:KLTH0F18964g"/>
    <dbReference type="eggNOG" id="KOG3393">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096876_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJT4"/>
    <dbReference type="OMA" id="VHITFVD"/>
    <dbReference type="OrthoDB" id="268928at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007919">
        <property type="entry name" value="UPF0220"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13180">
        <property type="entry name" value="SMALL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05255">
        <property type="entry name" value="UPF0220"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="24"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="105"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR24573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="176" mass="19079" checksum="9987090293487304" modified="2009-07-28" version="1">MEANQERLFRLPFKLPSSATARSLGVYISGGLYAIGAWCFLDAALYSRKANASSVHVTFIDWIPFICSTLGMIIVNSIEKNRLIQDALGSGSFSGSSSMAWQARVILFLGFALLAGGISGSIVVLILKFLVKDFTSYPTLGMGVNNVVGNFCVVLSCVLLWIAQNVEDEYSYSLTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJX5</accession>
    <name>C5DJX5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0F19866p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0F19866g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555051.1">
        <property type="nucleotide sequence ID" value="XM_002555005.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJX5"/>
    <dbReference type="FunCoup" id="C5DJX5">
        <property type="interactions" value="43"/>
    </dbReference>
    <dbReference type="GeneID" id="8293287"/>
    <dbReference type="KEGG" id="lth:KLTH0F19866g"/>
    <dbReference type="eggNOG" id="KOG3378">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035143_1_0_1"/>
    <dbReference type="InParanoid" id="C5DJX5"/>
    <dbReference type="OrthoDB" id="436496at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008941">
        <property type="term" value="F:nitric oxide dioxygenase NAD(P)H activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019825">
        <property type="term" value="F:oxygen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071500">
        <property type="term" value="P:cellular response to nitrosative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046210">
        <property type="term" value="P:nitric oxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01040">
        <property type="entry name" value="Mb-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.490.10">
        <property type="entry name" value="Globins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000971">
        <property type="entry name" value="Globin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009050">
        <property type="entry name" value="Globin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012292">
        <property type="entry name" value="Globin/Proto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044399">
        <property type="entry name" value="Mb-like_M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43396:SF6">
        <property type="entry name" value="ABL201WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43396">
        <property type="entry name" value="FLAVOHEMOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00042">
        <property type="entry name" value="Globin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46458">
        <property type="entry name" value="Globin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01033">
        <property type="entry name" value="GLOBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Globin" evidence="2">
        <location>
            <begin position="162"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="136"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="349"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="384"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS01033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR24614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="393" mass="42908" checksum="60BF67B1610A2981" modified="2009-07-28" version="1">MLKMMNVRQQPHIGPRPSTFATEHGPEAVARFDETLESTPSSTVFSKSFYEAYSDMDSSRTSVISDGEEPITRVSSGSSLESSIAHGTRYKLTMKLSPRDIMLVRESWSIMLDDECPPEKLKSFVSKLAAGRVLTTTETGASRSRRAGHQMGRTPLASIDQDQSAERAGPRARLATNIKSSNSLFGAQFLENIIALAPHLQTLFPTIKHAAVGITGVLTIAVNNLEDLSVMDGYLTSLGKRHARILGVTADQFEFAGAAFLQTVQERFGVACTVELEETWRRLYSFLANSLLQFGIDPTIELQTRDNEVVLMAPPELNGRVPAKKPETTAHAAARLQQKSLLDPVAMSREQRASGAPSMMPVGTSVPPRSVKRNAQPGFSKGRKSGEKDCVIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>C5DK23</accession>
    <name>C5DK23_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Autophagy-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0F01188g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Vacuolar effluxer which mediate the efflux of amino acids resulting from autophagic degradation. The release of autophagic amino acids allows the maintenance of protein synthesis and viability during nitrogen starvation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Vacuole membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ATG22 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554261.1">
        <property type="nucleotide sequence ID" value="XM_002554215.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DK23"/>
    <dbReference type="FunCoup" id="C5DK23">
        <property type="interactions" value="46"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DK23"/>
    <dbReference type="GeneID" id="8292452"/>
    <dbReference type="KEGG" id="lth:KLTH0F01188g"/>
    <dbReference type="eggNOG" id="ENOG502QR9I">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017518_1_0_1"/>
    <dbReference type="InParanoid" id="C5DK23"/>
    <dbReference type="OMA" id="QPWEIFP"/>
    <dbReference type="OrthoDB" id="42657at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032974">
        <property type="term" value="P:amino acid transmembrane export from vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17483">
        <property type="entry name" value="MFS_Atg22_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044738">
        <property type="entry name" value="Atg22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024671">
        <property type="entry name" value="Atg22-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050495">
        <property type="entry name" value="ATG22/LtaA_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23519">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23519:SF1">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11700">
        <property type="entry name" value="ATG22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="8 10" id="KW-0072">Autophagy</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <keyword evidence="4 10" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="29"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="93"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="128"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="237"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="298"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="334"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="368"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="400"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="464"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR23824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="503" mass="55738" checksum="BFACDC8F6482E590" modified="2009-07-28" version="1">MSYQAVTSDEEDQNRPEREVKTLNNIRGWYLYCFSSEPFIVSAVSTYVPLLLEQFARINGVLVKDHSVKCGSSDEKCVLGLLNNSVFIDTSSFALYTFSASVLFQAVLVISISGLVDMWNSVKFRKNVLIAFGVCGALSTIAISQLSSSQYYSLALLSILANCCYGVVNVVGNSLLPGFVRDLIDYNSHFKNSTVEDVTSVTSGRGSGVGYIGALIVQVCSIFLVRMSKAHDNIQVAVLLVGIWWLAWQFPLTWLLQDIIPPLIEDEQLQIRPSPKYLTYGWKSLYEALKHARLLRDVMIFLLGWFIISDSITTINSTAILFAKTELKMGTLGLIMVSILSLLSGIAGAFLIPQVLSYQLKQPPQRMMMSIIGWASVIPLYGTLGFFFDSVGLKHKGEMYMMAIWYGIAMGGLSAVSRTIFSLIIPSGKESTFFSLFSLTDKGSSVLGPFLAGLITDKTHNIRYSFYLLLFLLLISLPVFGMLNIERGKKEARELGDIQTLND</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKG1</accession>
    <name>C5DKG1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0F04400p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0F04400g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the replication factor A protein 3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554399.1">
        <property type="nucleotide sequence ID" value="XM_002554353.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKG1"/>
    <dbReference type="FunCoup" id="C5DKG1">
        <property type="interactions" value="169"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKG1"/>
    <dbReference type="GeneID" id="8292594"/>
    <dbReference type="KEGG" id="lth:KLTH0F04400g"/>
    <dbReference type="eggNOG" id="ENOG502S18S">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2098758_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKG1"/>
    <dbReference type="OMA" id="FRIIGQV"/>
    <dbReference type="OrthoDB" id="4040097at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031981">
        <property type="term" value="C:nuclear lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013970">
        <property type="entry name" value="Rfa2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08661">
        <property type="entry name" value="Rep_fac-A_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR23962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="116" mass="12419" checksum="726F9ED96C65F44B" modified="2009-07-28" version="1">MATSTPRVDPQQIGQLSSPVFRIIGQVTAQPQPTQIIVASPTTGGEMISLTNVQTSSNVNYELQAWYELVCRANDFGDAGFLVLDSVKCVFPPGESISVAGVVALQQLSSKFTEIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKG8</accession>
    <name>C5DKG8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0F04554p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0F04554g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554406.1">
        <property type="nucleotide sequence ID" value="XM_002554360.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKG8"/>
    <dbReference type="FunCoup" id="C5DKG8">
        <property type="interactions" value="29"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKG8"/>
    <dbReference type="GeneID" id="8292601"/>
    <dbReference type="KEGG" id="lth:KLTH0F04554g"/>
    <dbReference type="eggNOG" id="KOG2474">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023518_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKG8"/>
    <dbReference type="OMA" id="GTTCLKW"/>
    <dbReference type="OrthoDB" id="262547at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040:SF210">
        <property type="entry name" value="ZINC-REGULATED TRANSPORTER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040">
        <property type="entry name" value="ZINC/IRON TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="13"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="298"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="329"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="366"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="409"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="113"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="225"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="123"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="232"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR23969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="431" mass="46628" checksum="E1240D9909C4DC12" modified="2009-07-28" version="1">MTPSLFIEEIPRWARFTAISSLLCIAGSLCVPAIAALSRNKSGVNSRLLNYGLSVSAGSMITTSLYKMLPRGEDDKRVVFVGFFAGVVVSFLLNFVVHAYTSESLVHCGHASDNGEGSGAENVQDHARRHSEHEHNSEAEPLTSSARPALRNARSIIDLISKKDPNSGDCYGSMSCAPAGQSPSGQAEVTCQADNTTKAVVCPENGIGYDLENLALYREHFMKGNSHKHSHSSGSDSSNSSPNASVHSSEHHHHHLATPFSKLLSIGIQTCLVITLHKFPEGFIVFFTNNNDSDSKSFGFSIFLSLAMHNFIEGFAMTLPLYAAFRTKWYAILTASVLGGGSQPLGALIGYFIFKNTGAKELHINLLLSITSGFLFVIGLMMFQTAVGFSDSHHHHNDQERSPDETHSLATVCLKWCCFGVLLILGSGLFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKH6</accession>
    <name>C5DKH6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0F04730g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. NPR/HAL subfamily. HAL5 sub-subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554414.1">
        <property type="nucleotide sequence ID" value="XM_002554368.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKH6"/>
    <dbReference type="FunCoup" id="C5DKH6">
        <property type="interactions" value="230"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKH6"/>
    <dbReference type="GeneID" id="8292609"/>
    <dbReference type="KEGG" id="lth:KLTH0F04730g"/>
    <dbReference type="eggNOG" id="KOG0590">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016904_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKH6"/>
    <dbReference type="OMA" id="ECDIENA"/>
    <dbReference type="OrthoDB" id="6513151at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030003">
        <property type="term" value="P:intracellular monoatomic cation homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343">
        <property type="entry name" value="SERINE/THREONINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343:SF43">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE HAL5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="393"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="326"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="23"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="123"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="158"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="169"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="211"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="326"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR23977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="706" mass="78947" checksum="4B1712A5E32EFD92" modified="2009-07-28" version="1">MAMPKTSHGENSAGESELKRSRSLSNSIKTLFKSTSPSRKTTGPAEAHGGPKSGDKLMKLAKGKELELGEKRREAALPARGADTPPLASPAPAYRPHNAPGPKGVKGHILSHDFGKLSLSRHNNAANGGENSNGSQESIISEEGSVPKPDHQRKSSGKRSPNAESGTEGSDRELYDPERLEEESSIDNELSRDPNHVRRVKSLQRQKSGTSAASRSRSMSLSSRYRARSPTHTASPLDEHFESECKCVLSTEKFKLYEDGHHEHTLKVTPIVLETHDDDGKGKSMFSLSGFFKGHKEDEKLESALSLLPRANFDFRKRLSRILDEELNDSSSEPEDEQQKKVPKPVNPNAAIGTEELRLINKISRKIHDGGGKETSVEGAMAKKNYTLFQKYGKPVDIIGQGAYGVVKVCCIDISSLEDERSLDETYSNGQKLFYAVKELKPKNDEAREKFGTRLTSEFIIGHSLSQRGNSTQKAHPNILKMLDLMQTNDGFVEVLEFCPSGDLYSLLSSTSKKGLHPLEADCFMKQLLHGVQYMHDHGIAHCDLKPENILFGPNGTLKICDFGTSCVFQTAWEKQVHFQTGAVGSEPYVAPEEFIAEREYDPRLVDCWSCGVIYCTMVLGHYLWKIPLVDKDSVYRSFVEEVRANREFVAFEELKHVNAEVNRCRKLCLYAVFQWNPDKRTSIEKLLHSNWMKRTRCCVNYKTMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKM5</accession>
    <name>C5DKM5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">mitogen-activated protein kinase kinase</fullName>
            <ecNumber evidence="8">2.7.12.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0F05918g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.12.2" evidence="8"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554463.1">
        <property type="nucleotide sequence ID" value="XM_002554417.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKM5"/>
    <dbReference type="FunCoup" id="C5DKM5">
        <property type="interactions" value="290"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKM5"/>
    <dbReference type="GeneID" id="8292661"/>
    <dbReference type="KEGG" id="lth:KLTH0F05918g"/>
    <dbReference type="eggNOG" id="KOG0581">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_79_1_1"/>
    <dbReference type="InParanoid" id="C5DKM5"/>
    <dbReference type="OMA" id="NILCSAK"/>
    <dbReference type="OrthoDB" id="10252354at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004708">
        <property type="term" value="F:MAP kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071474">
        <property type="term" value="P:cellular hyperosmotic response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038066">
        <property type="term" value="P:p38MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000341">
        <property type="entry name" value="MAP kinase kinase PBS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48013">
        <property type="entry name" value="DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48013:SF25">
        <property type="entry name" value="MAP KINASE KINASE PBS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="256"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="16"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="162"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR24026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="562" mass="61022" checksum="C56F318ECAC30425" modified="2009-07-28" version="1">MGGLEPSNGDHEIKRTGSTSSNYSNINANLHARVKAFQEQRQLKRNGSVGSGHGSVSSQSGKTEGGGEPQNIDKIVNKPLPPLPPSKESGQVLSPIRRAPRPPALSAAGPGGGAAKPSLSARRGLKLPAGGMSLKMKPPAPQEFAGAPSNRNPAVATPGVATAQNGRRSNPGSLVNGVQTTSTSSRQQRDTEGTTPSQQQLQQQQSRSGSNGLFTNFSKYVDIKSGSLNFAGKLSLSSQGVDFSNGSSFRITLDELEFLEELGHGNYGNVSKVLHKPTNVIMAMKEVRLELDESKFTQILMELEVLHKCQSPFIVDFYGAFFIEGAVYMCMEYMDGGSLDKSYDSEYLGGIDEPQLAHITYAIIQGLKELKDVHNIIHRDVKPTNVLCSAAQGTVKLCDFGVSGNLVASLAKTNIGCQSYMAPERIKSLNPDKATYTVQSDIWSLGLSILEMAIGSYPYPVETFDNIFSQLSAIVDSPPPKLPQDRFSPVAQDFVNMCLQKVPERRRNYAALLEHPWLKRYSNIEVNMSEYITNRLEKKRLITEEKGSEPVKNIPALHKGGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKW2</accession>
    <name>C5DKW2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">1-phosphatidylinositol-4-phosphate 5-kinase</fullName>
            <ecNumber evidence="2">2.7.1.68</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">1-phosphatidylinositol 4-phosphate kinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Diphosphoinositide kinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">PIP5K</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0F08008g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14425"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58178"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.68"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.1.68" evidence="2"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554550.1">
        <property type="nucleotide sequence ID" value="XM_002554504.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKW2"/>
    <dbReference type="FunCoup" id="C5DKW2">
        <property type="interactions" value="282"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKW2"/>
    <dbReference type="GeneID" id="8292756"/>
    <dbReference type="KEGG" id="lth:KLTH0F08008g"/>
    <dbReference type="eggNOG" id="KOG0229">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004312_4_1_1"/>
    <dbReference type="InParanoid" id="C5DKW2"/>
    <dbReference type="OMA" id="HARIFPV"/>
    <dbReference type="OrthoDB" id="20783at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016308">
        <property type="term" value="F:1-phosphatidylinositol-4-phosphate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17303">
        <property type="entry name" value="PIPKc_PIP5K_yeast_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.800.10:FF:000009">
        <property type="entry name" value="Phosphatidylinositol 4-phosphate 5-kinase its3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.810.10">
        <property type="entry name" value="2-Layer Sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.800.10">
        <property type="entry name" value="Phosphatidylinositol Phosphate Kinase II Beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027483">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002498">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027484">
        <property type="entry name" value="PInositol-4-P-5-kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023610">
        <property type="entry name" value="PInositol-4/5-P-5/4-kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086:SF8">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 5-PHOSPHATE 4-KINASE, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01504">
        <property type="entry name" value="PIP5K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00330">
        <property type="entry name" value="PIPKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51455">
        <property type="entry name" value="PIPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PIPK" evidence="13">
        <location>
            <begin position="251"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="116"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="184"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="650"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="36"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00078403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00080374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00082306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR24113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="672" mass="76959" checksum="81B245679BCD32CD" modified="2009-07-28" version="1">MSLASQEFFPARDTSSDTPRSSQERRCYSSPTSVESYDKTKPQDFKDAAMSPLCPLDGDRRDSSGAAEQPAPGPLRQVRPRGGSAVDDQDSIIDRRSVVENGSFIQGLNDDDKVLLNSNTSRHKPSRRATQDSLRQFPFDSGKHAKIVPVADPEEESPGAAASPADAVADADAELEELDNVATLRRSRPSRASSTRSFGTSNRPGAISDGSLKRSESATAEIKKMRESLLHKREMRKKQRNFLDDDRVLIGNKVSEGHVNFTIAYNMLTGIRVAVSRCSGIMKPLTPRDFRMTKKLAFDFHGNELTPSSQYAFKFKDYCPEVFRELRALFGLDPADYLMSLTSKYILSELNSPGKSGSFFYFSRDYKYIIKTIHHAEHQHLRRTLKEYYTHIKENPDTLVSQFYGLHRVKMPISFKSKIKHRKIYFLVMNNLFPPHLEIHATFDLKGSTWGRYTKVYNPPQNPDEEAHRPVLKDLNWLEEQRSILFGPVKKKKFLQQLERDVDLLARLNIMDYSLLLGIHDMEKGNDLIDERNRLTMFNPSQPQLKEVRRKSVAEEQMRNKSKTAVERFFQQDEGGIRASDQFNNDLNTIYYVGIIDCLTNYSCIKKLETFWRGLNHDLNEISAVPPRDYGSRFYDFMAASVNRTPVQRYKDNPKVKEFKDSPAPRKTTKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKW3</accession>
    <name>C5DKW3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">P-type Na(+) transporter</fullName>
            <ecNumber evidence="19">7.2.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="ordered locus">KLTH0F08030g</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>K(+)(in) + ATP + H2O = K(+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:75815"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>Na(+)(in) + ATP + H2O = Na(+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:14634"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IID subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.3" evidence="19"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554551.1">
        <property type="nucleotide sequence ID" value="XM_002554505.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKW3"/>
    <dbReference type="FunCoup" id="C5DKW3">
        <property type="interactions" value="83"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKW3"/>
    <dbReference type="GeneID" id="8292757"/>
    <dbReference type="KEGG" id="lth:KLTH0F08030g"/>
    <dbReference type="eggNOG" id="KOG0202">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002360_3_0_1"/>
    <dbReference type="InParanoid" id="C5DKW3"/>
    <dbReference type="OMA" id="PVQKDCD"/>
    <dbReference type="OrthoDB" id="3352408at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008554">
        <property type="term" value="F:P-type sodium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02086">
        <property type="entry name" value="P-type_ATPase_Na_ENA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000016">
        <property type="entry name" value="Calcium-transporting P-type ATPase putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000015">
        <property type="entry name" value="Sodium ion P-type ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000039">
        <property type="entry name" value="Sodium P-type ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000047">
        <property type="entry name" value="Sodium P-type ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006414">
        <property type="entry name" value="P-type_ATPase_IID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01523">
        <property type="entry name" value="ATPase-IID_K-Na"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="16 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0915">Sodium</keyword>
    <keyword evidence="17" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="12" id="KW-1278">Translocase</keyword>
    <keyword evidence="6 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 23" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="68"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="284"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="312"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="812"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="849"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="886"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="935"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="988"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1021"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="24">
        <location>
            <begin position="14"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="500"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="500"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00035017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00035029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00049499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="CAR24114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1092" mass="120446" checksum="1CB848652E14C8FF" modified="2009-07-28" version="1">MPASPQTVPPLSQEFHTLSVRDTVHVLKTDANNGLRASDAQERLATYGENTLGDDSAIDFKSILIHQVCNAMILVLFISMVITLAIRDWISGGVIAFVVFINVAIGSFQEYKASKTMNSLKSLSTPSAHVVRDGSDETIASQQLVPGDLCVVKAGDTVPADLRLVECVNFETDEALLTGESLPIAKDSSQVYPATEDTPVGDRLNLAFASSTVSKGRATGIVVKTALDTEIGKIAQSLKGDNSLISKDENKSFWGNAAVTLKTTIGSFLGTTTGTPLHRKLSKLAVLLFFVAVVFAIVVMATQKFVVNKEVAIYAICVAVSMIPSSLVVVLTITMSVGAKIMATRNVVVRKLDSLEALGAVNDVCSDKTGTLTQGRMIVKQAWIPSFGTVTVSNSNDPFDPTDGNIELIPRFSPHEYNHNDTEDVGIITDFKNKFFADALPQELDSDLFMNWLKTATLANIAHVYQDPETGDWKAHGDPTEIAIQVFAHKMDFPRTALTLENQSDSESEVGSSTEKLPEQEAQYKHVAEFPFDSSVKRMSAVYDSLKENNRRHVFTKGAFERVLSCCTKWLPDGHDEKNARSMTQEDKDQVLKNVETLSSEGLRVLAFATKSFDEAQAQELGSKLTKDRDFVESELIFLGLVGIYDPPRPETAGAVKQFHKAGINVHMLTGDFPGTAKAIAQEVGILPSNLYHYPKEVVESMVMTAAQFDQLTDEEIDNLLVLPLVIARCAPQTKVRMIDALHRRDKFCAMTGDGVNDSPSLKKANVGIAMGINGSDVAKDASDIVLSDDNFASILNAVEEGRRMSDNIQKFVLQLLAENVAQALYLMIGLCFLDEQKLSVFPLSPVEVLWIIVVTSCFPAMGLGLEKSAPDIMEKEPKNSKVGIFTWEIILDMLVYGTWMAACCLACFVTVIYGKGNGQLGVDCNNSYSESCHYVFRGRAATFASMTWCALILAWEVIDMRRSFFKMQPETDTPYTQWMKDIWSNQFLFWSVIFGFLSVFPVVYIPVINDDVFKHKGIGYEWGLAFAYTVAFWIGAEVYKYFKRCYFKNKGKARNPENDLESKRVHDPFEKYNTAHSGSTTLQVTVGDYKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKW5</accession>
    <name>C5DKW5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0F08074p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0F08074g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554553.1">
        <property type="nucleotide sequence ID" value="XM_002554507.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKW5"/>
    <dbReference type="STRING" id="559295.C5DKW5"/>
    <dbReference type="GeneID" id="8292759"/>
    <dbReference type="KEGG" id="lth:KLTH0F08074g"/>
    <dbReference type="eggNOG" id="ENOG502RX7Y">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021649_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKW5"/>
    <dbReference type="OMA" id="CHNCLRL"/>
    <dbReference type="OrthoDB" id="3251668at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052360">
        <property type="entry name" value="Transcr_Regulatory_Proteins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36206">
        <property type="entry name" value="ASPERCRYPTIN BIOSYNTHESIS CLUSTER-SPECIFIC TRANSCRIPTION REGULATOR ATNN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36206:SF4">
        <property type="entry name" value="HYPOTHETICAL CONSERVED PROTEIN (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="9">
        <location>
            <begin position="568"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="225"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="352"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="393"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="521"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="606"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="14"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="50"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="117"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="225"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="402"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="428"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR24116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="635" mass="67624" checksum="42C77FACD5057773" modified="2009-07-28" version="1">MASEREPSDDPSVSPRSASPLKPASSAPGTPLAAVPPPQTSGLSAHIDSTRTPPDSQLDTAKPTSAPQNTPADSPEPALGLPDSAGRAVEHGSQRPHSSLPPPPIRGDRAGGGPPGEKIEAGKEEGVVKSDGKAAGAPDADVKSEGSGDSLDVTGNEEEKVAGDDAGSGGTGSDAGADEDEDEAAAARRMQLPHISTLLAMSREQDARGGPASNAVLARRSVFATSASRCSSSDSGSARETGSGVPAPALAVPGKVLGARKVLTPNRLYFNSHRSATDPSASPDHNYKINISSLLNSRGEDVYTLRSQEDPSSSPVFAGLVDSEDAGVVAPPTSQPRFINSKLDEVRSRVLLDPNSQSRPQPLPFAGAQWASRDDDSAAAGAAAAIITKMRSSPYGAHDDSNSSSRPGSASFRQHMRPVIRINQREYESDDSDENAIIEDDTEDELHDDYQRRGSNKISWNKSGMKKRVTRRQSAPSSNFKRRHSGYNDSAKRESRHSSTSSTSTVTSLLSAAALLGKVPSATSQEQGGAGQPRHKTLSVNHEPFLTPSSPKEKPRRKNNSGSRSRTGCWICRLRKKKCTEEKPSCHNCLRLNLECFYDHTKPDFISDTSKKNEKLEEIKKKTKEAKRMAMRRKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5DL19</accession>
    <name>C5DL19_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">L8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">RP24</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">YL11</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0F09284g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554607.1">
        <property type="nucleotide sequence ID" value="XM_002554561.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DL19"/>
    <dbReference type="FunCoup" id="C5DL19">
        <property type="interactions" value="1001"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DL19"/>
    <dbReference type="GeneID" id="8292814"/>
    <dbReference type="KEGG" id="lth:KLTH0F09284g"/>
    <dbReference type="eggNOG" id="KOG3255">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065464_0_0_1"/>
    <dbReference type="InParanoid" id="C5DL19"/>
    <dbReference type="OMA" id="CASHITN"/>
    <dbReference type="OrthoDB" id="10252633at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.930.12:FF:000003">
        <property type="entry name" value="60S ribosomal protein L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.930.12:FF:000004">
        <property type="entry name" value="60S ribosomal protein L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.930.12">
        <property type="entry name" value="Ribosomal protein L6, alpha-beta domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000702">
        <property type="entry name" value="Ribosomal_uL6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036789">
        <property type="entry name" value="Ribosomal_uL6-like_a/b-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020040">
        <property type="entry name" value="Ribosomal_uL6_a/b-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002359">
        <property type="entry name" value="Ribosomal_uL6_CS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655:SF16">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655">
        <property type="entry name" value="60S/50S RIBOSOMAL PROTEIN L6/L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00347">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002162">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56053">
        <property type="entry name" value="Ribosomal protein L6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00700">
        <property type="entry name" value="RIBOSOMAL_L6_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL6 alpha-beta" evidence="7">
        <location>
            <begin position="12"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL6 alpha-beta" evidence="7">
        <location>
            <begin position="97"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00075840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00080918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00082770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR24170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="191" mass="21593" checksum="9B106EF44CCA80CE" modified="2009-07-28" version="1">MKYIQTDQTLDIPEGVTVNIKSRVIKVTGPRGTLTKNLKHIDVTFAKVSNKQVKITVHNGDRKHVAALRTVKSLVANMVTGVTKGFKYKMRYVYAHFPINVNVVEKDGAKFVEIRNFLGDKQVRMVPVREGVSIDFSNNQKDEIVLSGNSIENVSQNAADIQQICRVRNKDIRKFLDGIYVSQKGFIEEEN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-11-03" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>C5DL53</accession>
    <name>GET3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATPase GET3</fullName>
            <ecNumber evidence="1">3.6.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Arsenical pump-driving ATPase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Arsenite-stimulated ATPase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Golgi to ER traffic protein 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Guided entry of tail-anchored proteins 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">GET3</name>
        <name type="ordered locus">KLTH0F10098g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors GET1 and GET2, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the GET1-GET2 receptor, and returning it to the cytosol to initiate a new round of targeting. Cooperates with the HDEL receptor ERD2 to mediate the ATP-dependent retrieval of resident ER proteins that contain a C-terminal H-D-E-L retention signal from the Golgi to the ER. Involved in low-level resistance to the oxyanions arsenite and arsenate, and in heat tolerance.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer. Component of the Golgi to ER traffic (GET) complex, which is composed of GET1, GET2 and GET3. Within the complex, GET1 and GET2 form a heterotetramer which is stabilized by phosphatidylinositol binding and which binds to the GET3 homodimer. Interacts with the chloride channel protein GEF1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
        <text evidence="1">GET1 and GET2 are required for targeting GET3 to the endoplasmic reticulum.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the arsA ATPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.-.-" evidence="1"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554641.1">
        <property type="nucleotide sequence ID" value="XM_002554595.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DL53"/>
    <dbReference type="SMR" id="C5DL53"/>
    <dbReference type="FunCoup" id="C5DL53">
        <property type="interactions" value="1032"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DL53"/>
    <dbReference type="GeneID" id="8292851"/>
    <dbReference type="KEGG" id="lth:KLTH0F10098g"/>
    <dbReference type="eggNOG" id="KOG2825">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040761_0_0_1"/>
    <dbReference type="InParanoid" id="C5DL53"/>
    <dbReference type="OMA" id="MDAPYEF"/>
    <dbReference type="OrthoDB" id="1770at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043529">
        <property type="term" value="C:GET complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071816">
        <property type="term" value="P:tail-anchored membrane protein insertion into ER membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02035">
        <property type="entry name" value="ArsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001359">
        <property type="entry name" value="ATPase GET3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03112">
        <property type="entry name" value="Asna1_Get3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025723">
        <property type="entry name" value="ArsA/GET3_ATPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016300">
        <property type="entry name" value="ATPase_ArsA/GET3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027542">
        <property type="entry name" value="ATPase_ArsA/GET3_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00345">
        <property type="entry name" value="GET3_arsA_TRC40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10803">
        <property type="entry name" value="ARSENICAL PUMP-DRIVING ATPASE ARSENITE-TRANSLOCATING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10803:SF3">
        <property type="entry name" value="ATPASE GET3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02374">
        <property type="entry name" value="ArsA_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword id="KW-0333">Golgi apparatus</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000388210" description="ATPase GET3">
        <location>
            <begin position="1"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="57"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="26"/>
            <end position="33"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03112"/>
        </source>
    </evidence>
    <sequence length="349" mass="39124" checksum="52B129166D51B243" modified="2009-07-28" version="1">MDLIAEPNLKELINSTTHKWIFVGGKGGVGKTTSSCSISIQMALAQPKKQFLLISTDPAHNLSDAFGEKFGKDARKVTGMDNLSCMEIDPSAALKDMNDMSVAQNDKNDGFSDLLQGGGLAELTGSIPGIDEALSFMEVMKHIKRQEEGEGEKYDTVIFDTAPTGHTLRFLQLPQTLSQLLQKFGEIAGRFGPMLNSLTGGGQNMDIMGKVDELKANVEKIREQFTNPDMTTFVCVCISEFLSLYETERLIQELMSYEMDVNSIIVNQLLFADDDAEHNCRRCQARWNMQKKYLDQIGELYDDFHVVKMPLCAGEIRGLNNLKKFSQFLNKEYDPVADNKIIYELEEQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLB1</accession>
    <name>C5DLB1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0F11528p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0F11528g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554699.1">
        <property type="nucleotide sequence ID" value="XM_002554653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLB1"/>
    <dbReference type="FunCoup" id="C5DLB1">
        <property type="interactions" value="45"/>
    </dbReference>
    <dbReference type="GeneID" id="8292912"/>
    <dbReference type="KEGG" id="lth:KLTH0F11528g"/>
    <dbReference type="eggNOG" id="ENOG502RNI7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039753_0_0_1"/>
    <dbReference type="InParanoid" id="C5DLB1"/>
    <dbReference type="OMA" id="AKYECLA"/>
    <dbReference type="OrthoDB" id="4021219at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="302"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="352"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="317"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR24262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="498" mass="55723" checksum="557579847C960E6B" modified="2009-07-28" version="1">MEYEDPIISHFKGQKEVSAETLAQLIDEQKHEQAAHKTRASPKKVHLQSANLRKCEKALSIFTDVIKLKFLIQKSWDLQALGKSVLVRFDIQTGALSSQEAKLDHAETFYRELALKTITRCGKLSKATEQLSLDLEDIGEHIRSKSDENPAACVENSACLLLEMWFLCGRVMRGLKRNVAALFIKSKLLLIDCELELMNSTAQGGYGNLQETIVSYRSFIKVLLQQLQDAESGNDQTLFEECLQVFLDVESMYGAMNLNWLLLETDQQESDMPSTTESRSAIFEEAAELRDIAQFVDNAVNEEKESGSDEFSSFTTEKLEPTHDRRMSETSSSSRISLMMERTSLTKELPHLLQAFNNAKRLEQELENVRAAKTEELETSMFGSPPPSLSSSMFSPSSSLFLASSPRYGNDMTATTYSTSSSMAFDDEEKSTENAPEKCTPIAHPRLVSSELSSHLIGSDFLRLSSLQQQPSSLRQPRSVQGFGSSVLNNLYGLGNRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLD2</accession>
    <name>C5DLD2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0F12012g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554720.1">
        <property type="nucleotide sequence ID" value="XM_002554674.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLD2"/>
    <dbReference type="FunCoup" id="C5DLD2">
        <property type="interactions" value="356"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLD2"/>
    <dbReference type="GeneID" id="8292934"/>
    <dbReference type="KEGG" id="lth:KLTH0F12012g"/>
    <dbReference type="eggNOG" id="KOG0605">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_67_4_1"/>
    <dbReference type="InParanoid" id="C5DLD2"/>
    <dbReference type="OMA" id="TVCNDRI"/>
    <dbReference type="OrthoDB" id="18472at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005816">
        <property type="term" value="C:spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000280">
        <property type="term" value="P:nuclear division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd21776">
        <property type="entry name" value="MobB_Sid2p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05600">
        <property type="entry name" value="STKc_Sid2p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000141">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000319">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000109">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF417">
        <property type="entry name" value="CELL CYCLE PROTEIN KINASE DBF2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="175"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="13">
        <location>
            <begin position="476"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="31"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="34"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR24283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="570" mass="65998" checksum="4099DE6FB8D02BE9" modified="2009-07-28" version="1">MYQKPANSRYREIESLTDNIGQLSFDAAVDSPKFQYQPSTPSKFRGENQSPSPTRSVNGADVSRNSMMDVDDSNADIDYTRSPKKLPLDFHKKASSNNTKRLVNVCQMYFLDYYCDMFDYVISRRERTRQVMRYLEQQRTQGVAGPEISKEWQAYLGKETGILRKRRLKPKNKDFEMITQVGQGGYGQVYLARKRDTREICALKILNKKLLMKLNETNHVLTERDILTTTRSEWLVKLLYAFQDPSSLYLAMEFVPGGDYRTLLINTRFLQAPHARFYISEMFCAVDALHQLGYTHRDLKPENFLIDSRGHIKLTDFGLAAGTVSMERIESMKIRLEEVKNLDFPKFEEKSMNDRRQMYKKLRDSDVNYASSTVGSPDYMALEVLEAKNYDFTVDYWSLGCILFESLVGYTPFSGSSTNETYENLRCWKHVLRRPRCDNGRYAFSDRTWELITRLIADPISRLRSFEHVKKMKYFAEISFENLRNVSPPFIPQLDNETDAGYFDDFTSEADMAKYADVFKRQDKLSAMVDDSAVVSKLIGFTYRHRKGKSGSSGVVYGGSEHADPFATFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLN6</accession>
    <name>C5DLN6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0G02200p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0G02200g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555134.1">
        <property type="nucleotide sequence ID" value="XM_002555088.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLN6"/>
    <dbReference type="FunCoup" id="C5DLN6">
        <property type="interactions" value="162"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLN6"/>
    <dbReference type="GeneID" id="8293396"/>
    <dbReference type="KEGG" id="lth:KLTH0G02200g"/>
    <dbReference type="eggNOG" id="ENOG502S308">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055876_0_0_1"/>
    <dbReference type="InParanoid" id="C5DLN6"/>
    <dbReference type="OMA" id="MACANEL"/>
    <dbReference type="OrthoDB" id="4067212at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="291"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="291"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="338"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="353"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="410"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="429" mass="50596" checksum="D9FA00326D5EEC22" modified="2009-07-28" version="1">MFHFNQSPCIFDREPAVEGFLPFTVAPDQKRRRVCCQPFYNNAMKRDRKGIRFDVTENQQEYVLSVYKNVAEDTMSKAICDQLQKLRNDRPPTYHVVRDFFGNEYYVQDHEDDEDFMRHAIANLDLSPIKRQVARQVFRDYEITLNHRGDELVLMSRKDGLFKEFSLGIEFEDVSVKGFDMISESVAVLKIGIKKAQKEVPQMYHIEFQPSSNDTQQQEKIEQQEQQKLIEAAQEEELAKREAAATLKQRERAQAAHYAQIKKQEELARKERAALREREEQRAIEAATLRKREEQREREVAALKKHEEEERAREQEAAQREEKRHAISQKKAFLVEQKRKRRQQERQRRAKSQKNEKQPEKKNISPSDSPHNVVININFPNAVSEKTSEKSRGRSRSPVVLEDVDDEEEFRYRRSLDKSPRGSSIIDDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLP7</accession>
    <name>C5DLP7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0G02442p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0G02442g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555145.1">
        <property type="nucleotide sequence ID" value="XM_002555099.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLP7"/>
    <dbReference type="FunCoup" id="C5DLP7">
        <property type="interactions" value="102"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLP7"/>
    <dbReference type="GeneID" id="8293407"/>
    <dbReference type="KEGG" id="lth:KLTH0G02442g"/>
    <dbReference type="eggNOG" id="ENOG502QT7N">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_280757_0_0_1"/>
    <dbReference type="InParanoid" id="C5DLP7"/>
    <dbReference type="OMA" id="EMLMCSG"/>
    <dbReference type="OrthoDB" id="3980110at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="641"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="17"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1113" mass="127750" checksum="2C9AEBF8918FF26F" modified="2009-07-28" version="1">MVRKKRSAVISDPPRRPSALPAPAEPNGTPGSPVGQPSREPRAFTDIDAQEKLVLGLFTTRPGLSTLRLYYELSCGRVNLLRYKKWQQSKPQRVSDAAYKRMVKRWTSSTEHVGLLMDHFLRNDNWALDYASFYSPAHKLSVAFLLDPETEVLVIDDEYNLLLDFALQCRALVEQLLNQIDLPSLLAKCAYGHGRLFQLCGRYVWYTFSAEQFDEAQDICYKYLSVLTDKLTAQQDLIPQIQPLFEIISSSKASHWVYGDGLFTENPESDSNSDSDHWPQKRLSAQERVYSFDLKDDGTLEASNVFNRTRRRHQALYQVLNLQKQNTAPLLSSQFFTLCALVDPVTQPTPNDSHIVSIDLLSDMFLGLLYSEINELHIDWRFHVCFNLQKIVQATLPRLNCHDFQRLNSVNNSDDSIDWRRNLHKWLPQGLNTQDLELIYMIDILAIYIIHKLYRDLPAQMNPFLASMISLWKNLTFVVLLGLEIDRFEEEQETFSTPVLVRAAIRGSSALRSVVATILNGHVEYKRHDFQHEPINIFMSPHGRKLCHGALYTDVRSHAAAMLALGIELEDVTNLLSDLQPGDRFDEDVKYMFDYEYDNYNEVDTEELDEDELEDVESRERIKEMRAYYKRCHCQFDDDELLPEDEEDGRPDASPYRVTRDAPPDNNVKLSNTSKPMALRSQKDSVEFDFNGRDWRGIPRGLNFYFNENYEFEKHLSSGQAHSLMCNAAEKKLPLEEGTQLLRVIATCVAKEQELTVLRSALLLGETPESENHSTLVADGDLTTDFVYEKWCENSMFEKILFHNETLVWRMMDEMLMCSGYRRVLIWFITHLEVSNSMIEYIYTLVLGNRGEKAASDGDYAKVPFSRQGAIVLSDIEIKMLLQEFFTNAAIFFSKQLRESLGDGEDDEDQGDDEKGSGISPHVVGLMKLVCYMVKSLMQKEMFDFKDPDYIFELQTLLMSWICILPEARDLFFALRSLVDEQSQNIKLESESIASACEPDSQSEPQLPQGTHAELEPEATHAVSIYNKKLMSLLPPAAGTENSAITALRSFISKHSLTTKTALFGRRVISQDDTIMPMYMSDREMDNRQFLAEFGIDYNDFVDGAYDSDFVYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DM54</accession>
    <name>C5DM54_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0G06094p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0G06094g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555302.1">
        <property type="nucleotide sequence ID" value="XM_002555256.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DM54"/>
    <dbReference type="FunCoup" id="C5DM54">
        <property type="interactions" value="113"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DM54"/>
    <dbReference type="GeneID" id="8293570"/>
    <dbReference type="KEGG" id="lth:KLTH0G06094g"/>
    <dbReference type="eggNOG" id="KOG0874">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043293_1_1_1"/>
    <dbReference type="InParanoid" id="C5DM54"/>
    <dbReference type="OMA" id="FFIFWDR"/>
    <dbReference type="OrthoDB" id="408954at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050307">
        <property type="entry name" value="Sterol_Desaturase_Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11863">
        <property type="entry name" value="STEROL DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Fatty acid hydroxylase" evidence="5">
        <location>
            <begin position="157"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR24865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="337" mass="39294" checksum="977169ED1962236F" modified="2009-07-28" version="1">MDMTNATYLGFSGQKGSFSFMDSDVAPEVTIRPKESLLPWLSDGCLSLALPVIAYWSFSMMFHAIDTLRLAEQFRIHPSQEVASRNKAGRMEVLREVLFQHFLQTVTGGLLVYFDPEPTTGFEARAMWEWKRALPFWVPNQAIYYGYMYGISLIKVFVGFCIIDSWQYWLHRLMHNNKTLYKMYHSRHHRLYVPYAYGALYNSPAEGFLLDTLGTGIAAIVTKLTHREQIALFTFATLKTVDDHCGYALPWDPFQWIFPNNAVYHDIHHQQFGIKTNFAQPFFTLWDQLCGTKFHGLEQYEKAQRRITIDKYKEFLKEREAEKQAKIDQGFGNKKDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMA7</accession>
    <name>C5DMA7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0G07304p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0G07304g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NUP186/NUP192/NUP205 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555355.1">
        <property type="nucleotide sequence ID" value="XM_002555309.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DMA7">
        <property type="interactions" value="224"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMA7"/>
    <dbReference type="GeneID" id="8293625"/>
    <dbReference type="KEGG" id="lth:KLTH0G07304g"/>
    <dbReference type="eggNOG" id="KOG1835">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002778_0_0_1"/>
    <dbReference type="InParanoid" id="C5DMA7"/>
    <dbReference type="OMA" id="AYGFIEW"/>
    <dbReference type="OrthoDB" id="2019644at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044611">
        <property type="term" value="C:nuclear pore inner ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006999">
        <property type="term" value="P:nuclear pore organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021827">
        <property type="entry name" value="Nup186/Nup192/Nup205"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31344">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31344:SF0">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11894">
        <property type="entry name" value="Nup192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR24918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1672" mass="187547" checksum="908C788DE2469207" modified="2009-07-28" version="1">MMEWATAPFEVVYTSIETDTFDTSKYEEVLPDLKTLNVLDGKAKNSASRAQLEKGQIKVPSGDTYQINQEFAIAAITLSDELNLDELLTAEIMLQNCDLDSVSENSVISLVNDAKVAFYVRRQYILQIVSFITNCCSSEDKVYIDLVSDGTLAKNIIPAFKQIESQLGEIKVLVNKSAILESYDTFAQQNVRFRRDFLLKEYDTLSQILYGLVKNGTLMKKDMLLELINCASMMDSGDFFIIYFLPALFLAISQLHLLPDGHVREMHQTLLLELESDTISVQPVKASLIFAFLAFFIGWCKAAPSQRAKSFNFATDVDEPMTKAVELGAIEQLMAFAADTSELDKDESIELFYDIRSLLERHIPRLYLKQLVDGDQPSPDAINNQRLLTNASMSQQSLSFFLSAFHHLLQVVITDCAFLLTKMKDAEEDSLLSGEDLFLDEISAKADLERFFITVHYFYAFRPDYSAVFWQDKESNAYGFIEWAMKCTDTLMRSCVFMMLSSLSFGPENSANVYHYVNLNANLSWNTVSQLISDYIVKISELERKLLDGQQRDREEQDPTTIALKTGLNEEVIILLSSLYTLIGCVAHDLNEGAKAQLSSLFTDILFEFVKVDTPLVGAALKVLSNLVPYDELERSKFWFNLDSWIFKGFRLTISDDSYREAFESILTHFQDITGFLQLLNSLLKVRTKSTNGYLEFGLLQFPPKLGHGYRKLGIWPYFDYVFHGILAQSNKLPNPAERAAIHKPILEILSSALSSFDYSVILNSIPSGSNLDELVVTDNFLTYAMESSTTAVLNYLFEENVFKNLLQNASFGVEKMGSITEEDCQSLALPCLALSTINQVLTIQETYVEELCPIVKKSASKNYFIPKDFGLHGLRSFYDAISFDLPLIVGIGLYVGHTNYKIASNSLDILDKISTEMKGRDSQAIVKDKLLSVFDSVDESARIKQAFINQLQTSITNYESFSIKMRILDFISKNLSYTDKQPTVAHFLLGLQIGNTISLGPDLSTFVTSGCSVLTSIIYLLQSSLMVLSSEEIEFLPAKLASSSMEILLKLCKNSATSKIVLRHMSESDFFETILARDPKVDVNTRWSGEVFAGNSAGDSLGFIKQTSTGAFLSFLSYRSFALQYLSLDIHGLSGESLKSRIKSRIDLLISNYLQPPRLFSFLDALNFDARPSPHETLKGLKVLSGINFNLPSLQKVTPCTELIYDCSELESLIRLRTQFLGKHLPLNFHEETITNKETLDRISGAEAATIEKHLTDYMSFEQFKSLQLSLLHSWVQLVQVVVLDGALPQVSISDFILRLFEAIVPKINDYVELDVCYSEELVSLCVFLYDLYQKDQKLNNGEHTADGRLIALFLACINGIRSPLSTLALRSDFYILANRFLVSIMKEVNTAKQVLKTLKLTSERLVEVVCNDAISGEGSSRITGILFLDSLVQIAGLNKVNFVLESLVKSNMLLLISHSIKTTDDLLSSGLEGITLDNLLYELTAFKSTIHFLIRIAESRSGAQALVENEIFRVIESCEFLEADPDLGLELIFTEVRTQESQAVRVTFSLDKSLNLSKDACGLSLFEIIVPVFQLMTSILISMGSANKPVLKKTRNLLIHFRKLVQGVLKRDALIEGSDEKHLDRSNSEGLQKLVKLVVLLCTLTGYNGGSQKNGLDPKIYTLPQSYGNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMQ2</accession>
    <name>C5DMQ2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0G10736p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0G10736g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the amino acid-polyamine-organocation (APC) superfamily. Amino acid/choline transporter (ACT) (TC 2.A.3.4) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555500.1">
        <property type="nucleotide sequence ID" value="XM_002555454.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMQ2"/>
    <dbReference type="FunCoup" id="C5DMQ2">
        <property type="interactions" value="211"/>
    </dbReference>
    <dbReference type="GeneID" id="8293778"/>
    <dbReference type="KEGG" id="lth:KLTH0G10736g"/>
    <dbReference type="eggNOG" id="KOG1289">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004495_0_3_1"/>
    <dbReference type="InParanoid" id="C5DMQ2"/>
    <dbReference type="OMA" id="TFHERYN"/>
    <dbReference type="OrthoDB" id="4476201at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015101">
        <property type="term" value="F:organic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000046">
        <property type="entry name" value="Amino-acid permease, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649">
        <property type="entry name" value="AMINO-ACID PERMEASE BAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649:SF6">
        <property type="entry name" value="GABA-SPECIFIC PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="72"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="107"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="149"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="197"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="226"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="272"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="305"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="362"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="415"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="440"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="481"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="513"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR25063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="568" mass="60990" checksum="7DAB674C1BB7DD3F" modified="2009-07-28" version="1">MEKVQAAANTTDVSSSIEPAADGLHQFGSLRTITSKRAGEVNFIDAKGANGDSELLAEIGYKQELNRHFSTIQVFGVAFSIMGLLPSIASVLAIGMPGGMVSLMWGWLIAGIFILAVGCGMAELASAIPTSGGLYYYTYYYSPEKYKAFLSFVIGNTNSLALVAGLCSITYGLAQEILSIVVVAKDGDFNITSGKTYGVFVAGIAATTAVTSLATVAVSKLQTVSILANVGLIVLFFIVLPIGVANTKGLNFNDGSFIFGKWQNETSWPDGWQFMMAGLQPAIWTIGAFDSCIHMSEEAKNATKAVPVGIISSIGVCWILGFIVCIVIAACMGPNIDAILESSYGSPLPQILFNALGKKWTIAFMTLIAACQFLMGCSILTAISRQIWAFARDDGLMFSRYIKVVNKKLSSPLRATWFGALCALVIGLLCLIGSTASNALFSLAIGGNYVAWMTPNLLRMTVGKDLFRPGAFFMGKLWSPIVNWIAIIFQTFILIMIMFPADSKDITPDTMNYTVVICGGVWIGSIVYFLVYKKKAFHGPKSNLSDEEYADAVGQDVIDQILSHQKSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMV3</accession>
    <name>C5DMV3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0G11902p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0G11902g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555551.1">
        <property type="nucleotide sequence ID" value="XM_002555505.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMV3"/>
    <dbReference type="FunCoup" id="C5DMV3">
        <property type="interactions" value="120"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMV3"/>
    <dbReference type="GeneID" id="8293831"/>
    <dbReference type="KEGG" id="lth:KLTH0G11902g"/>
    <dbReference type="eggNOG" id="KOG2056">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053889_0_0_1"/>
    <dbReference type="InParanoid" id="C5DMV3"/>
    <dbReference type="OMA" id="WRVVLKC"/>
    <dbReference type="OrthoDB" id="4033880at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030125">
        <property type="term" value="C:clathrin vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16993">
        <property type="entry name" value="ENTH_Ent5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276:SF5">
        <property type="entry name" value="EPSIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276">
        <property type="entry name" value="EPSIN/ENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01417">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="2">
        <location>
            <begin position="25"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="200"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="200"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="219"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="299"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="315"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="335"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="358"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR25114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="399" mass="44832" checksum="2A6EAD4544150996" modified="2009-07-28" version="1">MDSLSKKIQNLGIHDIRNAARFAQNVIVQYEPYQVDIRRATNTDSWGPTPKHLFKVMRNKYQVPLHLMTEYTLKRLVDHIASRPKNFYEKARKQYVNYGSEWRVVLKCLVVLEYLLLNVDNGSELDQVTSCLLTHKHILTRDVMQFRVDFSADGKMEVHERGIKKKCEQMIQYIEDPAYLKRERAKHNKNVAKIHQSSATFSTVSTPSTSGRDAVYGSYDDEDDDDDDGDYEDFRSAAPAQSAARRRTSAADEQRRQKREMLREKIKASELQRKASLKKAQEPPADLLDLDDPEPQPQRQSQPAAKPQASTSLGFDDDDDDDEFGDFQSDSHPQTTAPTSNNTTGTVSGLDLLDWDGPSTSAVPAASGAAAASTTRNGSSSNTQTDAFADLFSYSKSHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMZ5</accession>
    <name>C5DMZ5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tRNA-splicing endonuclease subunit Sen2</fullName>
            <ecNumber evidence="4">4.6.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0G12936g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the tRNA-intron endonuclease family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.16" evidence="4"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555593.1">
        <property type="nucleotide sequence ID" value="XM_002555547.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMZ5"/>
    <dbReference type="FunCoup" id="C5DMZ5">
        <property type="interactions" value="83"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMZ5"/>
    <dbReference type="GeneID" id="8294194"/>
    <dbReference type="KEGG" id="lth:KLTH0G12936g"/>
    <dbReference type="eggNOG" id="KOG4685">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012847_2_0_1"/>
    <dbReference type="InParanoid" id="C5DMZ5"/>
    <dbReference type="OMA" id="YSHPYWK"/>
    <dbReference type="OrthoDB" id="10249562at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000214">
        <property type="term" value="C:tRNA-intron endonuclease complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000213">
        <property type="term" value="F:tRNA-intron lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000379">
        <property type="term" value="P:tRNA-type intron splice site recognition and cleavage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22363">
        <property type="entry name" value="tRNA-intron_lyase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036167">
        <property type="entry name" value="tRNA_intron_Endo_cat-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006677">
        <property type="entry name" value="tRNA_intron_Endonuc_cat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006676">
        <property type="entry name" value="tRNA_splic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016589">
        <property type="entry name" value="tRNA_splic_SEN2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00324">
        <property type="entry name" value="endA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21227">
        <property type="entry name" value="TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21227:SF0">
        <property type="entry name" value="TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01974">
        <property type="entry name" value="tRNA_int_endo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF011789">
        <property type="entry name" value="tRNA_splic_SEN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53032">
        <property type="entry name" value="tRNA-intron endonuclease catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 4" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA intron endonuclease catalytic" evidence="7">
        <location>
            <begin position="234"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="147"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="263"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="271"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR011789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR011789-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR25156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="351" mass="41133" checksum="116112D4F8512303" modified="2009-07-28" version="1">MPKHVPNAKRYKYPLPIHPLDQLPELKLYNPVSWVYWLYCYTFSTNQLPSKIHAEFTHGKHLTIARPDDMRYLWENGFFGTAQLSRSEPTWRKRTMARLGLSEGSTALEHITEKRRIERLRFKQERSQFEAIKLDMRQRGVAEDHILAEERAFLKSLREKEKVLEEEGEVHLREVDEELFGPNEQLIDIEVLELMPVEGIFLTFALPVLEMNVKTLLANLAGDEPTYADIHELCLKYVAYHHYRSHGWCARSGIKFGCDYLLYRRGPPFQHAEYGVKVLEACKPFDTTLFTGAARVLSGARKPFILCYVELQKPESEVLESWRQGRLDKVFRSYKVGEVSYRRWVPGKNRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DN44</accession>
    <name>C5DN44_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Probable metalloprotease ARX1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Associated with ribosomal export complex protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0G14036g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Probable metalloprotease involved in proper assembly of pre-ribosomal particles during the biogenesis of the 60S ribosomal subunit. Accompanies the pre-60S particles to the cytoplasm.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M24 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555642.1">
        <property type="nucleotide sequence ID" value="XM_002555596.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DN44"/>
    <dbReference type="FunCoup" id="C5DN44">
        <property type="interactions" value="428"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DN44"/>
    <dbReference type="GeneID" id="8293926"/>
    <dbReference type="KEGG" id="lth:KLTH0G14036g"/>
    <dbReference type="eggNOG" id="KOG2776">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_477525_0_0_1"/>
    <dbReference type="InParanoid" id="C5DN44"/>
    <dbReference type="OMA" id="KPSWVHS"/>
    <dbReference type="OrthoDB" id="5876363at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047113">
        <property type="entry name" value="PA2G4/ARX1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10804:SF102">
        <property type="entry name" value="METALLOPROTEASE ARX1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10804">
        <property type="entry name" value="PROTEASE FAMILY M24 METHIONYL AMINOPEPTIDASE, AMINOPEPTIDASE P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00026155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR25205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="594" mass="64319" checksum="D987EE91FFE7623C" modified="2009-07-28" version="1">MALAISREDTQVLLHGKNVLEESTLDKYRSAGQITQTALKFVVKLINESYHADGKQRLAIHEICLLSDSFLTACLESSFKNKVAERGISHPTTLDVDEVASGWCPEVDDVEQLRSANKQMPDSASGCRRSVDGFLAPGDVVKVSLGVHIDGYTAQVSHTIVVYPTEDDESHGKVPTGPLLGGKADAYAAAHIAMETVVSLLACALTPEKLPRSLSSNNQVTGSLIRTVVDAIAASYKCVVVPGSKVRRVRRFLAGQNEGIVAEREYKGVAWYESHQELAMLQKSAESTKDLVAVKPSNRHNSSAVPTDEFTVNAGEVYVVDLQMAPLEGLVPGLITLETADNFSGKSHKADQLVARPGAYVRDFAHSHILKLKTSRQLLSKIDKQGVYPVKLSHLSDSFPIDTSCPESSQIEAISRDLKSYRLGMSEILNNFLAIPNPIRIAKCIPWDKILSTANPAGSLGVDATERSLPGQELPLPRLGISSLKLKSLLKHASSVPVARQSSTVVLCPSDVSSSGKAELLRLTGGKKTCQPSWIHSKYQMNQEDPLIQGVFQLAELSKDKRFGISIRETQPLKKENTLAGVAPSSATQDIDMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DN67</accession>
    <name>C5DN67_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0G14564p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0G14564g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the XPC family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555665.1">
        <property type="nucleotide sequence ID" value="XM_002555619.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DN67"/>
    <dbReference type="FunCoup" id="C5DN67">
        <property type="interactions" value="163"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DN67"/>
    <dbReference type="GeneID" id="8293950"/>
    <dbReference type="KEGG" id="lth:KLTH0G14564g"/>
    <dbReference type="eggNOG" id="KOG2179">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003639_1_2_1"/>
    <dbReference type="InParanoid" id="C5DN67"/>
    <dbReference type="OMA" id="IPEWLMS"/>
    <dbReference type="OrthoDB" id="300780at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000111">
        <property type="term" value="C:nucleotide-excision repair factor 2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071942">
        <property type="term" value="C:XPC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.20.110">
        <property type="entry name" value="Rad4, beta-hairpin domain BHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.290">
        <property type="entry name" value="Rad4, beta-hairpin domain BHD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2460">
        <property type="entry name" value="Rad4, beta-hairpin domain BHD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.260.10">
        <property type="entry name" value="Transglutaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018327">
        <property type="entry name" value="BHD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004583">
        <property type="entry name" value="DNA_repair_Rad4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018325">
        <property type="entry name" value="Rad4/PNGase_transGLS-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018326">
        <property type="entry name" value="Rad4_beta-hairpin_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018328">
        <property type="entry name" value="Rad4_beta-hairpin_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042488">
        <property type="entry name" value="Rad4_BHD3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036985">
        <property type="entry name" value="Transglutaminase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12135:SF0">
        <property type="entry name" value="DNA REPAIR PROTEIN COMPLEMENTING XP-C CELLS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12135">
        <property type="entry name" value="DNA REPAIR PROTEIN XP-C / RAD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10403">
        <property type="entry name" value="BHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10405">
        <property type="entry name" value="BHD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03835">
        <property type="entry name" value="Rad4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01030">
        <property type="entry name" value="BHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01031">
        <property type="entry name" value="BHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01032">
        <property type="entry name" value="BHD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad4 beta-hairpin" evidence="7">
        <location>
            <begin position="441"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="Rad4 beta-hairpin" evidence="8">
        <location>
            <begin position="500"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="domain" description="Rad4 beta-hairpin" evidence="9">
        <location>
            <begin position="558"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="41"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="296"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="675"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="707"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="783"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="51"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="62"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="99"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="794"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="839"/>
            <end position="858"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR25228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="858" mass="97857" checksum="9D7E61693B5258AC" modified="2009-07-28" version="1">MGQLPEEYFDLVREAVREDKRVPARKKRRLLRYDTKDVTGPANVVITLDSESEHENRERSSSPKGGISGSSTSEGDFAEDDDFNSDDFEDVTDLEGPEQTPQADVSITLNTSKKESKPRSKNLISNEDRKFRRYFHMFHLLALLVHAHIRNQWLNDHKLHAKLSKLVPDDVFEMLHPKKDNELPLRSTRKLLDGLKKCMEIWWKHFDQITRWETPGMNMIEWKELDGPWEQPARFMSQKLFSKKVAHGKGSSDVAAQGFVAMLRACGVNARLIINAQPPDFTDNKVSMPGNVKHAGEYLEDSSSRPLPRRRQNKKEKDSDAKRYSIFWCEVWDKASKKWITVDPMGQRIIEQIRYKTTLEPQGKARRNNLFRYIIAFDRKQGCRDVTRRYTTHFNSKTRRRRITRDLEGEEWFRRILAKLHRRKRTRTDDFEDAYFQQRDETEGIPDNMQDLKNHPYYVLQNDLKWNEVLKSGCKECGFLRTKNNTSSLKVFRRSDVLVLKTARTWYTEGRVLKTGAAALKTTNSRDFRTGETTEERLYSFDQTELFVPENLGPNNEVPTNVYGNIDVYAPNMIPQGSCLIESPVAVKAAALLGIEFAKAVTGFKFEKRSAKPQFTGIVVAQEYQEAVESMIDGVEYSQEEDKRREHELESLQYWNLFLAKLRIKQRLNKTHGKVISGDQHAESGHSAGELGGNNDVEVAEDDSFEAGGFMPEADGNMPDAGGFIPDAEGNISEAGGFVAEASELPAEAGTFGFESNELVSGVDDPSNGGEGFLPEENEMAEGCNEIGGDDLEVSTNFNRSLPRSSEFDAGLSRVENEQKPTSDGMDDDYEEFMKDINFEDEQGLSAQDDSDFSYESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNE5</accession>
    <name>C5DNE5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Inorganic pyrophosphatase</fullName>
            <ecNumber evidence="4">3.6.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Pyrophosphate phospho-hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0G16302g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>diphosphate + H2O = 2 phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24576"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="EC" id="3.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.1" evidence="4"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555743.1">
        <property type="nucleotide sequence ID" value="XM_002555697.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNE5"/>
    <dbReference type="FunCoup" id="C5DNE5">
        <property type="interactions" value="1097"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNE5"/>
    <dbReference type="GeneID" id="8294029"/>
    <dbReference type="KEGG" id="lth:KLTH0G16302g"/>
    <dbReference type="eggNOG" id="KOG1626">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040684_0_2_1"/>
    <dbReference type="InParanoid" id="C5DNE5"/>
    <dbReference type="OMA" id="LYANEQK"/>
    <dbReference type="OrthoDB" id="1608002at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004427">
        <property type="term" value="F:inorganic diphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006796">
        <property type="term" value="P:phosphate-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00412">
        <property type="entry name" value="pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.80.10:FF:000004">
        <property type="entry name" value="Inorganic pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.80.10">
        <property type="entry name" value="Inorganic pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008162">
        <property type="entry name" value="Pyrophosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036649">
        <property type="entry name" value="Pyrophosphatase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10286">
        <property type="entry name" value="INORGANIC PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00719">
        <property type="entry name" value="Pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50324">
        <property type="entry name" value="Inorganic pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00387">
        <property type="entry name" value="PPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR25306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="286" mass="32064" checksum="638A3F95615EF005" modified="2009-07-28" version="1">MVYTTRQIGAKNTLDYKVYIEENGKPISSFHDIPLYADEANQIFNMVVEIPRWTNAKLEVSKEETLNPIIQDTKKGKLRYVRNCFPHHGYIHNYGAFPQTWEDPNSIHPETKAAGDNDPLDVLEIGETIGYTGQVKQVKVLGVMALLDEGETDWKVIVIDINDPLAPKLNDIEDVEKYFPGLLRATNEWFRIYKIPDGKPENQFAFSGEAKNKKYALDVIRECSDAWKNLIQGKSDVKGIELTNTTLSGTSTYSPSAGSAVAAAEQKEDAPVDKSVDKWFFISGSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5E211</accession>
    <name>C5E211_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Adenylate kinase isoenzyme 6 homolog</fullName>
            <shortName evidence="10">AK6</shortName>
            <ecNumber evidence="10">2.7.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Dual activity adenylate kinase/ATPase</fullName>
            <shortName evidence="10">AK/ATPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0H01298g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Broad-specificity nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates. Has also ATPase activity. Involved in the late cytoplasmic maturation steps of the 40S ribosomal particles, specifically 18S rRNA maturation. While NMP activity is not required for ribosome maturation, ATPase activity is. Associates transiently with small ribosomal subunit protein uS11. ATP hydrolysis breaks the interaction with uS11. May temporarily remove uS11 from the ribosome to enable a conformational change of the ribosomal RNA that is needed for the final maturation step of the small ribosomal subunit. Its NMP activity may have a role in nuclear energy homeostasis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>AMP + ATP = 2 ADP</text>
            <dbReference type="Rhea" id="RHEA:12973"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with small ribosomal subunit protein uS11. Not a structural component of 43S pre-ribosomes, but transiently interacts with them by binding to uS11.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the adenylate kinase family. AK6 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.3" evidence="10"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555934.1">
        <property type="nucleotide sequence ID" value="XM_002555888.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E211"/>
    <dbReference type="FunCoup" id="C5E211">
        <property type="interactions" value="884"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E211"/>
    <dbReference type="GeneID" id="8294247"/>
    <dbReference type="KEGG" id="lth:KLTH0H01298g"/>
    <dbReference type="eggNOG" id="KOG3347">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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        <property type="component" value="Chromosome H"/>
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        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:AMP kinase activity"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="P:ribosomal small subunit biogenesis"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="P:rRNA processing"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="FunFam" id="3.40.50.300:FF:000372">
        <property type="entry name" value="Adenylate kinase isoenzyme 6 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00039">
        <property type="entry name" value="Adenylate_kinase_AK6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020618">
        <property type="entry name" value="Adenyl_kinase_AK6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12595:SF0">
        <property type="entry name" value="ADENYLATE KINASE ISOENZYME 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12595">
        <property type="entry name" value="POS9-ACTIVATING FACTOR FAP7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13238">
        <property type="entry name" value="AAA_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4 10" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="NMPbind" evidence="10">
        <location>
            <begin position="38"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="LID" evidence="10">
        <location>
            <begin position="113"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="176"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="188"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
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        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>ATP</name>
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        </ligand>
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    <feature type="binding site" evidence="10">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR30072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="214" mass="24024" checksum="C0EA45297E9EF5C8" modified="2009-07-28" version="1">MLSTRFAPNILVTGTPGCGKTSTCELLQRRLKDANYYNISDFAQQHECYDGYDEARKSHIVDEDRLLDELEPLMRKGGAIVDWHVNDIFPERLIDLVVVLRTDNTVLYDRLKSRGYHEAKIQENIDAEIMGVVIQDAQDSYEKEIVVELQSDTADQMDENVDRIASWSAAWLEQNPKGVTNELVERGSDEEDGTDEEGGSYGNSASSGEDGEDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2H0</accession>
    <name>C5E2H0_LACTC</name>
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        <submittedName>
            <fullName evidence="2">KLTH0H04884p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0H04884g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002556047.1"/>
    </dbReference>
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    <dbReference type="STRING" id="559295.C5E2H0"/>
    <dbReference type="GeneID" id="8294407"/>
    <dbReference type="KEGG" id="lth:KLTH0H04884g"/>
    <dbReference type="eggNOG" id="ENOG502SASQ">
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    </dbReference>
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    <dbReference type="InParanoid" id="C5E2H0"/>
    <dbReference type="OMA" id="WEIQINE"/>
    <dbReference type="OrthoDB" id="5873279at2759"/>
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        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="CDD" id="cd02859">
        <property type="entry name" value="E_set_AMPKbeta_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR30231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="217" mass="24760" checksum="84FF623E6E41C6D7" modified="2009-07-28" version="1">MSKIVFKHQQAGLGSLSLAGNFTKWEIKPMVFNGDEGQWEFEIEPEMLGNCELQDGKAVTFFKFVDERGDWFTDDNFAKEADENGNENNALYLQVDGDAARSENQPSEVEHQTEGDQEAEPSTPDPTPVVQMKTPRANEREESPVMITESDLEEDFHDTNNQDQGELTETRNHSADTSVSATQNPAEYKNFLQSIVFFFKNLFRRWFGMGTKQQESR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2M4</accession>
    <name>C5E2M4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0H06160p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0H06160g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat CDC20/Fizzy family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556147.1">
        <property type="nucleotide sequence ID" value="XM_002556101.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2M4"/>
    <dbReference type="FunCoup" id="C5E2M4">
        <property type="interactions" value="242"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2M4"/>
    <dbReference type="GeneID" id="8294940"/>
    <dbReference type="KEGG" id="lth:KLTH0H06160g"/>
    <dbReference type="eggNOG" id="KOG0305">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014831_6_0_1"/>
    <dbReference type="InParanoid" id="C5E2M4"/>
    <dbReference type="OMA" id="TWSDDDC"/>
    <dbReference type="OrthoDB" id="10263272at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010997">
        <property type="term" value="F:anaphase-promoting complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990757">
        <property type="term" value="F:ubiquitin ligase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905786">
        <property type="term" value="P:positive regulation of anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000098">
        <property type="entry name" value="WD repeat-containing protein slp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033010">
        <property type="entry name" value="Cdc20/Fizzy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056150">
        <property type="entry name" value="WD40_CDC20-Fz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918">
        <property type="entry name" value="CELL DIVISION CYCLE 20 CDC20 FIZZY -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918:SF8">
        <property type="entry name" value="FI02843P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24807">
        <property type="entry name" value="WD40_CDC20-Fz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 7" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="CDC20/Fizzy WD40" evidence="9">
        <location>
            <begin position="229"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="358"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="495"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="31"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="39"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="50"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="91"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF24807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR30285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="574" mass="63321" checksum="7AFA4D7D8A6CE2E9" modified="2009-07-28" version="1">MADNRRNKLDFANNASKSVLSLTSPAKLNVVSSDWSKMTSKRPSGKISKRQASSGGTLSNVTNIPTNSKSSGSRPTLQYVPPLLRRSSSLYKEDLESGERKTIKSQDSEEGFLNTDRFIPLRKENSFSHSKIDPMNLEEEVPPPNASPSTHLKAQTKRVFKQNVAEACGLEMNQRILQYLPKPPAASHRKQTYSMGSRSHYSYSAVQNPGSSQELAKLRKINSNPERILDAPGFQDDFYLNLVSWSNKNVLAIALDSALYLWNGSSGDVSLLVDFEQPGSITSVTWSDDDCHISIGKLEGNTEIWDIETMSHVRTMRSGLGVRIGSQSWLETLIATGAKSGEIHINDVRIKNHIVSTWEEHRGEVCGISYRPDGLQVASGSNDNTVVIWDTRTSLPQHIKRQHTAAVKALAWSPDINNLLATGGGQTDKHIHFWNTTTGARTGSINTGSQVSSLHWGQSYNANTIQREIVATGGNPENAVSVYNYDTKFKVAEIVHAHEARICSSQLSPDGTVLATVGGDENLKFYKVFEPRRKRPQGVCDGESLLGLLNVGEACDDDPTRSPNRRKTSEFLIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2Q4</accession>
    <name>C5E2Q4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0H06842p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0H06842g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556177.1">
        <property type="nucleotide sequence ID" value="XM_002556131.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2Q4"/>
    <dbReference type="FunCoup" id="C5E2Q4">
        <property type="interactions" value="100"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2Q4"/>
    <dbReference type="GeneID" id="8294493"/>
    <dbReference type="KEGG" id="lth:KLTH0H06842g"/>
    <dbReference type="eggNOG" id="KOG0118">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016304_5_0_1"/>
    <dbReference type="InParanoid" id="C5E2Q4"/>
    <dbReference type="OMA" id="HTSERQL"/>
    <dbReference type="OrthoDB" id="446113at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050825">
        <property type="entry name" value="RBM42_RBP45_47-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47640:SF10">
        <property type="entry name" value="TRNA SELENOCYSTEINE 1-ASSOCIATED PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47640">
        <property type="entry name" value="TRNA SELENOCYSTEINE 1-ASSOCIATED PROTEIN 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="175"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="406"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="42"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="121"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="263"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="527"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="42"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="281"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="297"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR30315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="547" mass="59536" checksum="217531E9310B0CF4" modified="2009-07-28" version="1">MGDLDPSFDEATIQRIWAHLGKRVSVKLIRAKKNLLIPCSTSSTFSDTQPQAPAPEGSGALRAPDADGAGRKIHINGVSFIDPNTTQLHHAGYCFVEFESLADAQWALTLNSTPLPDITCPGPAAADMASPAGSPAAGPDASSPLRTNPTGLRNFRLNWASGATLLSAIPSTPEFSLFVGDLSPTATEAHLLSLFQKKFKSVKTVRVMTDPITGASRCFGFVRFGDEQERRRALVEMNGVWCQGRNLRVAYATPRNNVMWQLTQPQHQQRQPQHHQHQHQHQQQQQQQQQQQQQLHHQQHQQHQQHHHIQNHLSQYHQHYQSMSQNNFQLASPEYTQAPLLMRTASSLVGNNLINFPATDFVSGSDAPYMGVPSMPPSLAHASAAASGAASAPAPGRSPFTNPNNTTVFIGGLTSHTSERQLHSLFAPFGTIINVKIPPGKGCGFVKYAYRIDAEAAIQGMQGFIVGGNPIRLSWGRTSTDTSRHPAMQDMPSSLRNVASQRGPQVWNSATAPTPTTAVSMFASPHIQRQDSVTQAGSVPPVHPSLL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-06-28" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2S6</accession>
    <name>SHU2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName>Suppressor of hydroxyurea sensitivity protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">SHU2</name>
        <name type="ordered locus">KLTH0H07348g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plays a role in a RAD51/RAD54-dependent homologous recombination repair (HRR) pathway to repair MMS-induced lesions during S-phase. Required for error-free repair of spontaneous and induced DNA lesions to protect the genome from mutation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SHU2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556199.1">
        <property type="nucleotide sequence ID" value="XM_002556153.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2S6"/>
    <dbReference type="SMR" id="C5E2S6"/>
    <dbReference type="FunCoup" id="C5E2S6">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2S6"/>
    <dbReference type="GeneID" id="8294516"/>
    <dbReference type="KEGG" id="lth:KLTH0H07348g"/>
    <dbReference type="eggNOG" id="ENOG502S0XB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1115918_0_0_1"/>
    <dbReference type="InParanoid" id="C5E2S6"/>
    <dbReference type="OMA" id="WLKLHLN"/>
    <dbReference type="OrthoDB" id="4066852at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0233">DNA recombination</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0539">Nucleus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000409734" description="Suppressor of hydroxyurea sensitivity protein 2">
        <location>
            <begin position="1"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="68"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="249" mass="28279" checksum="2E334D50101DB7FE" modified="2009-07-28" version="1">MVDEHYIDYSQLLAQLLDKDGELNETTVSFLYHLFPSDFFVRAMSLIDSNNMFIYVFERDETSAALTTSVGTEPDEVETTTETLRTSPSPPVASEQELGNAVQSSALINTLYEKPQSVLHRLIVKQEGPQSPPVCVDLRHWFCSCDEYNTLFEERMLTDEEPSLYSKATTELRAGASITDSFASMPPKGASRRYFRHDIIMCPHLLAFAILLQTTPELLTYFTHKAATVYLITIQNLDEWLKLHLNVVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>C5E304</accession>
    <name>C5E304_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 10">Uricase</fullName>
            <ecNumber evidence="7 10">1.7.3.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Urate oxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0H09262g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 10">Catalyzes the oxidation of uric acid to 5-hydroxyisourate, which is further processed to form (S)-allantoin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 10">
            <text>urate + O2 + H2O = 5-hydroxyisourate + H2O2</text>
            <dbReference type="Rhea" id="RHEA:21368"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
            <dbReference type="ChEBI" id="CHEBI:18072"/>
            <dbReference type="EC" id="1.7.3.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Purine metabolism; urate degradation; (S)-allantoin from urate: step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7 10">Belongs to the uricase family.</text>
    </comment>
    <dbReference type="EC" id="1.7.3.3" evidence="7 10"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556277.1">
        <property type="nucleotide sequence ID" value="XM_002556231.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E304"/>
    <dbReference type="STRING" id="559295.C5E304"/>
    <dbReference type="GeneID" id="8294602"/>
    <dbReference type="KEGG" id="lth:KLTH0H09262g"/>
    <dbReference type="eggNOG" id="KOG1599">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048151_0_0_1"/>
    <dbReference type="InParanoid" id="C5E304"/>
    <dbReference type="OMA" id="ATMYKMS"/>
    <dbReference type="OrthoDB" id="9992118at2759"/>
    <dbReference type="UniPathway" id="UPA00394">
        <property type="reaction ID" value="UER00650"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004846">
        <property type="term" value="F:urate oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006145">
        <property type="term" value="P:purine nucleobase catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019628">
        <property type="term" value="P:urate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.270.10:FF:000001">
        <property type="entry name" value="Uricase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.270.10">
        <property type="entry name" value="Urate Oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002042">
        <property type="entry name" value="Uricase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019842">
        <property type="entry name" value="Uricase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03383">
        <property type="entry name" value="urate_oxi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42874">
        <property type="entry name" value="URICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42874:SF1">
        <property type="entry name" value="URICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01014">
        <property type="entry name" value="Uricase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000241">
        <property type="entry name" value="Urate_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00093">
        <property type="entry name" value="URICASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55620">
        <property type="entry name" value="Tetrahydrobiopterin biosynthesis enzymes-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00366">
        <property type="entry name" value="URICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6 7" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="4 7 10" id="KW-0659">Purine metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Charge relay system" evidence="8">
        <location>
            <position position="13"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8">
        <location>
            <position position="267"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>5-hydroxyisourate</name>
            <dbReference type="ChEBI" id="CHEBI:18072"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>O2</name>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>urate</name>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>5-hydroxyisourate</name>
            <dbReference type="ChEBI" id="CHEBI:18072"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>urate</name>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>5-hydroxyisourate</name>
            <dbReference type="ChEBI" id="CHEBI:18072"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>urate</name>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>5-hydroxyisourate</name>
            <dbReference type="ChEBI" id="CHEBI:18072"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>urate</name>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>5-hydroxyisourate</name>
            <dbReference type="ChEBI" id="CHEBI:18072"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>urate</name>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>5-hydroxyisourate</name>
            <dbReference type="ChEBI" id="CHEBI:18072"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>urate</name>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>5-hydroxyisourate</name>
            <dbReference type="ChEBI" id="CHEBI:18072"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>O2</name>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>urate</name>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000241-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000241-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR30415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="307" mass="34745" checksum="A002CAF3A61A0A1E" modified="2009-07-28" version="1">MPYSYLESSTYGKDNVKFLKVKKDKTNPKVHEIMEATVCVLLKGDFEISYTEADNAPIVPTDTVKNTILILAKKYDTFPIEKFAAKLALHFTSKYSHVSGLTVKITQDRWVKFDVDGKESQHSFVHQGPEKKITFLDYDKKNGPSNYSLSSSIKDLTVLKSTNSMFYGYNVCDYTTLKPTTDRILSTDIFATMDWCPKKLGCLESIASGSKDEIFTNAYNSARDITLDIFAKENSPSVQATMYNMATAILKIAPQVAYVSYELPNKHYFLFDLKWFQGLENDNELFYPSPHPNGLIKCKVGRNGSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5E360</accession>
    <name>C5E360_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein BTN</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0H10626g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Vacuole membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the battenin family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556333.1">
        <property type="nucleotide sequence ID" value="XM_002556287.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E360"/>
    <dbReference type="FunCoup" id="C5E360">
        <property type="interactions" value="128"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E360"/>
    <dbReference type="GeneID" id="8294664"/>
    <dbReference type="KEGG" id="lth:KLTH0H10626g"/>
    <dbReference type="eggNOG" id="KOG3880">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029663_1_2_1"/>
    <dbReference type="InParanoid" id="C5E360"/>
    <dbReference type="OMA" id="WLCNWQV"/>
    <dbReference type="OrthoDB" id="5965864at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003492">
        <property type="entry name" value="Battenin_disease_Cln3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018460">
        <property type="entry name" value="Battenin_disease_Cln3_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10981">
        <property type="entry name" value="BATTENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10981:SF0">
        <property type="entry name" value="BATTENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02487">
        <property type="entry name" value="CLN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015974">
        <property type="entry name" value="CLN3_BTN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01315">
        <property type="entry name" value="BATTENIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="70"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="93"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="298"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="327"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="362"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR30471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="402" mass="44516" checksum="6C03C119BF479322" modified="2009-07-28" version="1">MVESSTVFRYFWIFGLVNNVLYVVILSAAADIVGPNLPKSLILLVDILPSFTVKLSAPFFIHKVPHNRRIALLIILSCVGMILVSGGKLSVCIGGIILASLSSGLGEITFLQLTHYYQHPSLNGWSSGTGGAGLLGSGAYLLLTSILKVPVGISLLLFSVLPFAFLLYFRLDTQDSYQPIEGEITESAQPSTASSDSFPDNYKATSKWMALKNHINTTLTRLRSLFIPYMLPLSTVYLFEYLINQAVSPTLLFPLDGDHTHPFFFNKYRDIYVTYGTLYQLGVFISRSSGSFIRIKKLFLLSVLQGLNLTVVICQSWFYIVHSVYPVMLLIFFEGLLGGASYVHTFMNIIHDVNPSEREFTLGAVSMSDSFGTLLAAFIGIALEPKLCSHQVDTGRDWCLKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3A0</accession>
    <name>C5E3A0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0H11594p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0H11594g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ornithine cyclodeaminase/mu-crystallin family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556373.1">
        <property type="nucleotide sequence ID" value="XM_002556327.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3A0"/>
    <dbReference type="FunCoup" id="C5E3A0">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3A0"/>
    <dbReference type="GeneID" id="8294707"/>
    <dbReference type="KEGG" id="lth:KLTH0H11594g"/>
    <dbReference type="eggNOG" id="KOG3007">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042088_0_1_1"/>
    <dbReference type="InParanoid" id="C5E3A0"/>
    <dbReference type="OMA" id="CVGMGLM"/>
    <dbReference type="OrthoDB" id="41492at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1780.10">
        <property type="entry name" value="ornithine cyclodeaminase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003462">
        <property type="entry name" value="ODC_Mu_crystall"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023401">
        <property type="entry name" value="ODC_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13812">
        <property type="entry name" value="KETIMINE REDUCTASE MU-CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13812:SF19">
        <property type="entry name" value="KETIMINE REDUCTASE MU-CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02423">
        <property type="entry name" value="OCD_Mu_crystall"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR30511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="333" mass="36367" checksum="66A0C5A4546773E6" modified="2009-07-28" version="1">MCSAIIQDAQVQKHFLNVSHKALCRYIDQLQIALHRYSSRPEIIPPRLVDTTMNGNTTHLIMPVLDDVFSGLKWLGYNPQGGLGFVGAVTVTDPRSGGLIGVVQAKQLTGVRTALASCIGIRNQLSAFKNKTSITVFGTGLQSFWHIFVCAKLLEGREIAVNVIYRSSPMDTALLEDHLPSLKVAQFQLSDTEQVRDKVSQSNIIFGCVPSTEPSILKKYLEASSGPEYTYISLIGSYKSHMHECDTALVEQFKDQGVQILVDSKEHTLIEAGELIDANVAPTQLLEIGNLEPNKPLPKSTCKNGKLVTLCKIVGLAIMDVSMAKTLLADLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3B7</accession>
    <name>C5E3B7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Isocitrate dehydrogenase [NADP]</fullName>
            <ecNumber evidence="11">1.1.1.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">KLTH0H12012g</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>D-threo-isocitrate + NADP(+) = 2-oxoglutarate + CO2 + NADPH</text>
            <dbReference type="Rhea" id="RHEA:19629"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.42"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11 14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="11 14">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="11 14">Binds 1 Mg(2+) or Mn(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.42" evidence="11"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556390.1">
        <property type="nucleotide sequence ID" value="XM_002556344.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3B7"/>
    <dbReference type="FunCoup" id="C5E3B7">
        <property type="interactions" value="823"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3B7"/>
    <dbReference type="GeneID" id="8294726"/>
    <dbReference type="KEGG" id="lth:KLTH0H12012g"/>
    <dbReference type="eggNOG" id="KOG1526">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023296_1_1_1"/>
    <dbReference type="InParanoid" id="C5E3B7"/>
    <dbReference type="OMA" id="ACVECID"/>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004450">
        <property type="term" value="F:isocitrate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006097">
        <property type="term" value="P:glyoxylate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006102">
        <property type="term" value="P:isocitrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006739">
        <property type="term" value="P:NADP+ metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.718.10:FF:000002">
        <property type="entry name" value="Isocitrate dehydrogenase [NADP]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.718.10">
        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004790">
        <property type="entry name" value="Isocitrate_DH_NADP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00127">
        <property type="entry name" value="nadp_idh_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006156">
        <property type="entry name" value="PRK08299.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11822:SF41">
        <property type="entry name" value="ISOCITRATE DEHYDROGENASE [NADP]-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11822">
        <property type="entry name" value="NADP-SPECIFIC ISOCITRATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000108">
        <property type="entry name" value="IDH_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0329">Glyoxylate bypass</keyword>
    <keyword evidence="6 11 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 11 14" id="KW-0464">Manganese</keyword>
    <keyword evidence="5 11 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11 15" id="KW-0521">NADP</keyword>
    <keyword evidence="8 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="16">
        <location>
            <begin position="9"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="75"/>
            <end position="77"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="94"/>
            <end position="100"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="312"/>
            <end position="317"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="site" description="Critical for catalysis" evidence="12">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <feature type="site" description="Critical for catalysis" evidence="12">
        <location>
            <position position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR000108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000108-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000108-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000108-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000108-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAR30528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="413" mass="46523" checksum="2B3A8A0B33984746" modified="2009-07-28" version="1">MAKIRVANPIVEMDGDEQTRIIWHLIREKLIMPFLDIDLKYYDLSIQNRDATDDEVTAASARATLKYGVAVKCATVTPDEARVREFGLKRMWASPNGTIRNILGGTVFREPIVIPRIPRLVPGWQRPIIIGRHAFGDQYKATDVAIEGAGELRLVFRSRDGDQAKDLDLQVYEFPESGGVALAMYNTTESIRGFARASFELALQRKMPLYSTTKNTILKRYDGRFKDEFEAMYEAEYRERFERAGIWYEHRLIDDMVAQMLKSAGGFVIAMKNYDGDVQSDIVAQGFGSLGLMTSVLVTPDGRAFESEAAHGTVTRHFRLHQQGKETSTNSIASIFAWTRGLLQRGRLDGTPEVEGFARALETATVATVNEDGIMTKDLALMLGRTDRASYVTTEDFIDAVEQRLIRSLKEDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBE1</accession>
    <name>C5DBE1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0A01848p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0A01848g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M20A family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551540.1">
        <property type="nucleotide sequence ID" value="XM_002551494.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBE1"/>
    <dbReference type="GeneID" id="8290335"/>
    <dbReference type="KEGG" id="lth:KLTH0A01848g"/>
    <dbReference type="eggNOG" id="ENOG502QPR4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024588_2_0_1"/>
    <dbReference type="InParanoid" id="C5DBE1"/>
    <dbReference type="OMA" id="IWPHGRW"/>
    <dbReference type="OrthoDB" id="4676at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016813">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amidines"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03884">
        <property type="entry name" value="M20_bAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010158">
        <property type="entry name" value="Amidase_Cbmase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01879">
        <property type="entry name" value="hydantase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32494:SF5">
        <property type="entry name" value="ALLANTOATE AMIDOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32494">
        <property type="entry name" value="ALLANTOATE DEIMINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001235">
        <property type="entry name" value="Amidase_carbamoylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="3">
        <location>
            <begin position="247"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR21098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="453" mass="49426" checksum="E246B16C8FF29259" modified="2009-07-28" version="1">MSTKSTTTTDLTCESGILNIPAAAPLAINPGRLNQTILETGTAYGGVARWGSQEHEFGMRRLAGTEEDGKMRGWFIKECEGLGCKIKVDQIGNIFAIFPGKNGGNPTATGSHLDTQPEAGKYDGILGVLAGLEVLRTFKENNYTPNYDVCVVVWFNEEGARFARSCTGSSVWSHDLPLKDAYELMSIGESIPESVYESLSKIGYIGEVSASYQENAIDAHFELHIEQGPILEDEKKEIGIVTGVQAYRWEKCTVFGVGAHAGTTPWRCRKDALLASSKMIVAASEIAKKHQGLMTCGVIDAKPYSVNIIPGEVSFTIDIRHPSDEVVAKIFSEVHAQFDLIVKDNVAGALTYDLEVLQVSPAVNFNEVCIECVSRSALAQFPASKVRQIWSGAGHDSCQTALHVPTSMIFIPSKDGLSHNFYEYSSAEEVENGFKVLLQAIVNYDNHRAIRGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBH7</accession>
    <name>C5DBH7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0A02728p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0A02728g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551576.1">
        <property type="nucleotide sequence ID" value="XM_002551530.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBH7"/>
    <dbReference type="GeneID" id="8290375"/>
    <dbReference type="KEGG" id="lth:KLTH0A02728g"/>
    <dbReference type="eggNOG" id="ENOG502SFSU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_145613_0_0_1"/>
    <dbReference type="InParanoid" id="C5DBH7"/>
    <dbReference type="OMA" id="VNIVIEY"/>
    <dbReference type="OrthoDB" id="4064992at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="89"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR21134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="137" mass="15308" checksum="443C6C756689DB89" modified="2009-07-28" version="1">MENDGSSSGLLRNRNVARSGKTSEERVFGPRFKSKLLKVQNQPVSTLGVFISACSIALSAVAMFLLGVNIVIQYRTIVPEDRAGMTRKASYIVLSAGAIYLLNKFNLWFFRKVVSYFSSGPYDRLPAQESFEMEQRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBJ3</accession>
    <name>C5DBJ3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phenylalanine--tRNA ligase alpha subunit</fullName>
            <ecNumber evidence="5">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Phenylalanyl-tRNA synthetase alpha subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">KLTH0A03102g</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>tRNA(Phe) + L-phenylalanine + ATP = L-phenylalanyl-tRNA(Phe) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="5"/>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551592.1">
        <property type="nucleotide sequence ID" value="XM_002551546.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBJ3"/>
    <dbReference type="FunCoup" id="C5DBJ3">
        <property type="interactions" value="1111"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBJ3"/>
    <dbReference type="GeneID" id="8290392"/>
    <dbReference type="KEGG" id="lth:KLTH0A03102g"/>
    <dbReference type="eggNOG" id="KOG2784">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025086_2_0_1"/>
    <dbReference type="InParanoid" id="C5DBJ3"/>
    <dbReference type="OMA" id="QIEGWVM"/>
    <dbReference type="OrthoDB" id="238316at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009328">
        <property type="term" value="C:phenylalanine-tRNA ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00496">
        <property type="entry name" value="PheRS_alpha_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2320:FF:000001">
        <property type="entry name" value="phenylalanine--tRNA ligase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2330:FF:000002">
        <property type="entry name" value="Phenylalanyl-tRNA synthetase alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000028">
        <property type="entry name" value="Phenylalanyl-tRNA synthetase alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004529">
        <property type="entry name" value="Phe-tRNA-synth_IIc_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002319">
        <property type="entry name" value="Phenylalanyl-tRNA_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040586">
        <property type="entry name" value="PheRS_DBD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040725">
        <property type="entry name" value="PheRS_DBD3"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00468">
        <property type="entry name" value="pheS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003210">
        <property type="entry name" value="PRK04172.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538:SF40">
        <property type="entry name" value="PHENYLALANINE--TRNA LIGASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538">
        <property type="entry name" value="PHENYLALANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18554">
        <property type="entry name" value="PheRS_DBD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18553">
        <property type="entry name" value="PheRS_DBD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01409">
        <property type="entry name" value="tRNA-synt_2d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0436">Ligase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="17">
        <location>
            <begin position="227"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00015409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00030612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAR21150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="499" mass="56956" checksum="FACFAB27B27929A0" modified="2009-07-28" version="1">MSDLQLEILQKLDQLGEIQSTLEVFPNINPQVVLSSLNSLKAHGKLEYTKQDKTYYTLSKEGEEIMGKGSHEIRLLQLVDKFTKLQIKDVATHMGADGKVGQARAFKNGWISKNKDNELTVSDKISSVNDVQDETKDILVQIKNGNYEGVNDKTLNDLKKRKLVAAKKEISFHVTKGADFSTELTKLETDLTADMVASGTYKDLKFKPYNFNSEGILPESGALHPLNKVREEFRQIFFSMGFTEMPANQYVDTGFWNFDALYVPQKHPARDLQDTFYLKDPIKSDLPEDKAYLENIKAVHEQGKFESIGYRYDWKPEESQKLVLRTHSTATSAYMLKKLAEDPKPTRLFSIDRVFRNEAVDATHLAEFHQVEGVLADYNITLGDLIQFMEGFFEKMGVTGLRFKPTYNPYTEPSMEIFSWHEGLGKWVEIGNSGMFRPEMLESMGLPPNMRVLGWGLSLERPTMIKYGVQNIRELLGHKVSLDFIETNPAARLDEDLYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBJ8</accession>
    <name>C5DBJ8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Enhancer of polycomb-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0A03212g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of selected genes principally by acetylation of nucleosomal histone H4 and H2A. The NuA4 complex is also involved in DNA repair. Involved in gene silencing by neighboring heterochromatin, blockage of the silencing spreading along the chromosome, and required for cell cycle progression through G2/M.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the enhancer of polycomb family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551597.1">
        <property type="nucleotide sequence ID" value="XM_002551551.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBJ8"/>
    <dbReference type="FunCoup" id="C5DBJ8">
        <property type="interactions" value="297"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBJ8"/>
    <dbReference type="GeneID" id="8290397"/>
    <dbReference type="KEGG" id="lth:KLTH0A03212g"/>
    <dbReference type="eggNOG" id="KOG2261">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010580_0_0_1"/>
    <dbReference type="InParanoid" id="C5DBJ8"/>
    <dbReference type="OMA" id="MLGTKSY"/>
    <dbReference type="OrthoDB" id="435275at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024943">
        <property type="entry name" value="Enhancer_polycomb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019542">
        <property type="entry name" value="Enhancer_polycomb-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14898">
        <property type="entry name" value="ENHANCER OF POLYCOMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10513">
        <property type="entry name" value="EPL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Enhancer of polycomb-like N-terminal" evidence="10">
        <location>
            <begin position="15"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="709"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="414"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="725"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="747"/>
            <end position="761"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF10513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR21155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="761" mass="88007" checksum="166FD7E00DBAF732" modified="2009-07-28" version="1">MAAPSSQEAANTRFRHRKISVKQRLQVYKASDLRKLDKDELQQRELVEIETGVEKNEEKEEHLHKILQKNQLTRKELYIPTPDASQTWGEYENFYRGKFEEPVSYIRFSATVEDCCGSCYNMDERDERFLESKVNNKLKDGEKLSETEFELMCSSLEAAIHERQPFLSIDPENILSLEEIKPTILKRDLGDAGIKDKLAEEIGVQPQHFITQFDSAVQQDSRPITVLFEKFGKQVYNHWKGRKIEAHGGKICPQLKSERPGDRDDNDPYVCFRRREVRQARKTRRVDYQNSHKLRLLHQQLLFTKKLALLVAKREKVSLDMLENDLRVFELRRDIKPIKRSAVIRGDDDLLLDQKKRRLVSNVVTSTALESNPKKLRTKSKKANADAANSKVAVAAKPQSRSSQAQSQQSAQQLHSQQQALLQQQQAQQQAQQQKQQANAIAAHVYVKLPSSKIPDILLEDVDDILLKKERSARNYVEERMRKRRLEDGERFFNLTDDPYNPVFDISVPAGVSPTNAPFSSIASSKFEIEKSYYVPGLDNYLEGKADDVVVLNRDGDRIDNPKYRKIERYRPFQQNSGVHTRELPFRLRRRVGRLGLEYIDRRRPSEAFEPLKSFINLAEVEKQERENDVIDVYDSKLDALSRLHDRWKHDSDYNDHGAKFSHEPARLNQISNDTQVIRFGTMLGTKSYEQLRDATFKYRQEIYNQRRIQKNSISQKHPRVNGVTPASASTPSMSSTPARTSSSSSIKRNSNTKQHVASIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBL0</accession>
    <name>C5DBL0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0A03476p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0A03476g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551609.1">
        <property type="nucleotide sequence ID" value="XM_002551563.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBL0"/>
    <dbReference type="SMR" id="C5DBL0"/>
    <dbReference type="FunCoup" id="C5DBL0">
        <property type="interactions" value="518"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBL0"/>
    <dbReference type="GeneID" id="8290409"/>
    <dbReference type="KEGG" id="lth:KLTH0A03476g"/>
    <dbReference type="eggNOG" id="KOG1577">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_0_0_1"/>
    <dbReference type="InParanoid" id="C5DBL0"/>
    <dbReference type="OMA" id="EMGIVVI"/>
    <dbReference type="OrthoDB" id="416253at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000002">
        <property type="entry name" value="2,5-diketo-D-gluconic acid reductase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11732">
        <property type="entry name" value="ALDO/KETO REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00798">
        <property type="entry name" value="ALDOKETO_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00063">
        <property type="entry name" value="ALDOKETO_REDUCTASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="5">
        <location>
            <begin position="30"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="4">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR21167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="308" mass="34507" checksum="7B6609B89098BC18" modified="2009-07-28" version="1">MSAPKPSTSVGKLNTGASIPLVGLGTYLSEDETGYKAVLHAIKVGYRHIDCAALYKNEDFVGRAIRDSGVPREELFVTTKVWNTQHREPAKALEESLSRLGLDYVDLFLMHWPQALRTERITDGNMITVPKREDEKPDVDTEWNYIKTWHLMQDLPASGKTKAVGVSNFSVKQLEKLLADPGSKVVPACNQVEVHPLLPQIPLYDFCKKKNIMLAAYSPLGSQKAPLLDDPVILEIAAKHKADPAQIIIGWDVKRGYVALPKSVTPSRIESNLQIVNLTDSELEKINEIHKKGEKRVVDPEWFDFEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBR7</accession>
    <name>C5DBR7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">40S ribosomal protein S24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0A04818g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS24 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551666.1">
        <property type="nucleotide sequence ID" value="XM_002551620.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBR7"/>
    <dbReference type="FunCoup" id="C5DBR7">
        <property type="interactions" value="1254"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBR7"/>
    <dbReference type="GeneID" id="8290470"/>
    <dbReference type="KEGG" id="lth:KLTH0A04818g"/>
    <dbReference type="eggNOG" id="KOG3424">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107248_1_0_1"/>
    <dbReference type="InParanoid" id="C5DBR7"/>
    <dbReference type="OMA" id="IRVKKYM"/>
    <dbReference type="OrthoDB" id="5571754at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3370:FF:000001">
        <property type="entry name" value="40S ribosomal protein S24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00545">
        <property type="entry name" value="Ribosomal_eS24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053709">
        <property type="entry name" value="eRP_eS24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001976">
        <property type="entry name" value="Ribosomal_eS24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018098">
        <property type="entry name" value="Ribosomal_eS24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_uL23/eL15/eS24_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10496">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01282">
        <property type="entry name" value="Ribosomal_S24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="Ribosomal proteins S24e, L23 and L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00529">
        <property type="entry name" value="RIBOSOMAL_S24E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="102"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="105"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="124"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR21224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="135" mass="15299" checksum="705936BA86E7C56A" modified="2009-07-28" version="1">MSDAITIRTRKVITNPLLARKQFVVDVLHPNRANVAKDELREKLAEAYKAEKDAVSVFGFRTQFGGGKSVGFGLIYNSVADAKKFEPTYRLVRYGLAEKVEKASRQQRKQKKNRDKKVFGTGKRLAKKVARRNAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBV4</accession>
    <name>C5DBV4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">G1/S-specific cyclin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0A05632g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Essential for the control of the cell cycle at the G1/S (start) transition. Interacts with the CDC28 protein kinase to form MPF.</text>
    </comment>
    <comment type="function">
        <text evidence="6">G1/S-specific cyclin essential for the control of the cell cycle at the G1/S (start) transition.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the cyclin family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551703.1">
        <property type="nucleotide sequence ID" value="XM_002551657.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBV4"/>
    <dbReference type="FunCoup" id="C5DBV4">
        <property type="interactions" value="383"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBV4"/>
    <dbReference type="GeneID" id="8290507"/>
    <dbReference type="KEGG" id="lth:KLTH0A05632g"/>
    <dbReference type="eggNOG" id="KOG0653">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_536434_0_0_1"/>
    <dbReference type="InParanoid" id="C5DBV4"/>
    <dbReference type="OMA" id="WDVYEPM"/>
    <dbReference type="OrthoDB" id="5590282at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007089">
        <property type="term" value="P:traversing start control point of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20559">
        <property type="entry name" value="CYCLIN_ScCLN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000080">
        <property type="entry name" value="G1/S-specific cyclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014399">
        <property type="entry name" value="Cyclin_CLN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21615">
        <property type="entry name" value="CYCLIN N-TERMINAL DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21615:SF2">
        <property type="entry name" value="CYCLIN N-TERMINAL DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001770">
        <property type="entry name" value="Cyclin_CLN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 6" id="KW-0132">Cell division</keyword>
    <keyword evidence="3 6 7" id="KW-0195">Cyclin</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="8">
        <location>
            <begin position="78"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00053308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR001770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR21261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="504" mass="56523" checksum="4B279829CF10C259" modified="2009-07-28" version="1">MGVSNGIPQGLIVTAKQTYYPIELSNAELLAHYEMIQDYHQEISANVISQSCKFKPDPKLIDQQPEMNPQQTRQQVMAVLFELSVKTRVTNGIFFHAARLYDRYCSKRVVLREQAKLVIATCLWLAAKTWGGCNHIINNVSVPTGGRFYGPNPRARIPRLSELVHYCGGADVFDESMFMQMERHILDTLSWDIFEPMVNDYVLNVDENCLLQYELYKRQLEHNKEWFHRASQSSDSDATEADEEEDEDLVDKIQLINIKKFLIDLATWQYDLLKFEMFEVAHGIFAIINKFTGQDQGPLLLSPLPTSTNQSLLLRIFINAVVKAPKQLLQFYENSQGVNQFVAAVRDFHASRQKKVQIASGMDLSGRPTVCYEVPSMPSPPHPNVNYTPMRNTSAPSDVSIFSAAESQPSPVTPVMYSFANNKTNSGSACGSVLSVNSLSNKRSRADDYDCMDEDKENQPHNPTFAPPPRAKFVGNSIFASGGTSATNSNRSSLVSLAISNNIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DC07</accession>
    <name>C5DC07_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0A06864p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0A06864g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">May be involved in regulation of tRNA subcellular distribution.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. 6-phosphogluconolactonase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551756.1">
        <property type="nucleotide sequence ID" value="XM_002551710.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DC07"/>
    <dbReference type="FunCoup" id="C5DC07">
        <property type="interactions" value="263"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DC07"/>
    <dbReference type="GeneID" id="8290563"/>
    <dbReference type="KEGG" id="lth:KLTH0A06864g"/>
    <dbReference type="eggNOG" id="KOG3147">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053947_0_1_1"/>
    <dbReference type="InParanoid" id="C5DC07"/>
    <dbReference type="OMA" id="KLAWCLP"/>
    <dbReference type="OrthoDB" id="432544at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017057">
        <property type="term" value="F:6-phosphogluconolactonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006409">
        <property type="term" value="P:tRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01400">
        <property type="entry name" value="6PGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1360:FF:000017">
        <property type="entry name" value="6-phosphogluconolactonase-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005900">
        <property type="entry name" value="6-phosphogluconolactonase_DevB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039104">
        <property type="entry name" value="6PGL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006148">
        <property type="entry name" value="Glc/Gal-6P_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01198">
        <property type="entry name" value="pgl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11054">
        <property type="entry name" value="6-PHOSPHOGLUCONOLACTONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11054:SF0">
        <property type="entry name" value="6-PHOSPHOGLUCONOLACTONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01182">
        <property type="entry name" value="Glucosamine_iso"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucosamine/galactosamine-6-phosphate isomerase" evidence="7">
        <location>
            <begin position="101"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="43"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="43"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="70"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00054376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR21314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="343" mass="37093" checksum="62DCB1DF6DF143B4" modified="2009-07-28" version="1">MTTTVPRVFAFHEFAGVAEAVADHVVHAQNLALSKEPYALKNTGGSNSTPANSSSLNVPGSNGAGNAPVSRQSSSRSIGHSHSAAGSGLRGPSSSKLKKIKEKRFKIALSGGSLIQVLHEGLLKRQDVLWGKWDIYFADERLVPFESSESNFGNTKRKILDLIDTCTYGTPRVYHINESLINDPQECADDYEKELIKGFAGRDSVKLPMFDLFLLGCAPDGHIASLFPNFSENLRENLAWVVPVTNAPSGPSNRISLTIPVICHSHQVTFVVEGATKAPVVKTIMERPEKGLPSSIVNEGAAGRVAWFVDDDALTDVQVTKKKYKFLVNTDASQISPSPPVPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DC36</accession>
    <name>C5DC36_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">KLTH0A07502p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0A07502g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551785.1">
        <property type="nucleotide sequence ID" value="XM_002551739.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DC36">
        <property type="interactions" value="1308"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DC36"/>
    <dbReference type="GeneID" id="8290592"/>
    <dbReference type="KEGG" id="lth:KLTH0A07502g"/>
    <dbReference type="eggNOG" id="KOG1831">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000286_3_1_1"/>
    <dbReference type="InParanoid" id="C5DC36"/>
    <dbReference type="OMA" id="VECHYQL"/>
    <dbReference type="OrthoDB" id="1933107at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
        <property type="term" value="C:CCR4-NOT core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000288">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20710">
        <property type="entry name" value="NOT1_connector"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.840">
        <property type="entry name" value="CCR4-NOT transcription complex subunit 1 TTP binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007196">
        <property type="entry name" value="CCR4-Not_Not1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032191">
        <property type="entry name" value="CNOT1_CAF1_bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024557">
        <property type="entry name" value="CNOT1_dom_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032194">
        <property type="entry name" value="CNOT1_HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032195">
        <property type="entry name" value="CNOT1_HEAT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032193">
        <property type="entry name" value="CNOT1_TTP_bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038535">
        <property type="entry name" value="CNOT1_TTP_bind_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040398">
        <property type="entry name" value="Not1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13162">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13162:SF8">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16415">
        <property type="entry name" value="CNOT1_CAF1_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16418">
        <property type="entry name" value="CNOT1_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16419">
        <property type="entry name" value="CNOT1_HEAT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16417">
        <property type="entry name" value="CNOT1_TTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12842">
        <property type="entry name" value="DUF3819"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04054">
        <property type="entry name" value="Not1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1 HEAT repeat 1" evidence="13">
        <location>
            <begin position="168"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1 HEAT repeat" evidence="12">
        <location>
            <begin position="406"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1 TTP binding" evidence="11">
        <location>
            <begin position="591"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1 CAF1-binding" evidence="10">
        <location>
            <begin position="781"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1" evidence="9">
        <location>
            <begin position="1103"/>
            <end position="1245"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-Not complex component Not1 C-terminal" evidence="8">
        <location>
            <begin position="1776"/>
            <end position="2142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1036"/>
            <end position="1087"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="1322"/>
            <end position="1397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1051"/>
            <end position="1065"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1077"/>
            <end position="1087"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF16418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF16419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR21343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="2169" mass="246701" checksum="326D7D07059245B9" modified="2009-07-28" version="1">MHTSPELQQSQRSKEVKEVAQITLTQISILITSLDESNCKKIENEILSLLERSPFVVYLKYWKKLLPLASDFIKQHRRLNTSSEPLHRLLYSLIQDLEFKNREVIEILTKEVFQNAEFQAQTSLNFTDLLALLDPEEDKRIIEQIDPEAAIKRLKKLRNLKMNAAQSLQDFLASETPDTLEQNLSSLLCSLKGESLNDSVALILAEILSPGSQNIQLPSTAWFTPNAISEASQRGTQISNAFTKLGPSAINWNRVFNLMSTKYFLNRPISISLASLNSLLAALNRGRLIDQFLSCDWNMQFKLSVCLLFHKWNLQQGCIDLLSMPDIKKVTNNIQNSSPKNTLLYLKSVARLDVEIFLLREELAGNPLLPAFQECFFEDYDLAPEYLALVVMSDNKHFTMLVDNKSIIDDLLITLLVKVFENSPAALTDLLKQLPFEKVVDMGSIIIRKDSSPLLNLVQVLSEVNILPEFIKTLPLHEALKVTPSAKKLGWIGFEDYMKSQLSAETVPLILDHLELQSKMDESSTTFYSSKVYDLSALHYLISLLNSFPLSGKERENLENVQFSLIIAFPRLINFGHGHDKAIIANGDSVPIPMDVEKEMQNYLQKMYSGELAIKDVIEVLRKLRDSDDPHDQDLFACMTHAVIAESNFFKDYPLEALATTSVLFGSMILFQLLRGFVLDVAFRVILNFAQDGTESKMFKFSVQALYAFRMRLKEFPQYCKHLIEAVPALQSQPQIYQTLSQAAAQSPERSNNGATKVQKPPEMIALKYFAISEVPPKVLQESPPKETTEKILFIVNNITQDNFEDKIEALKLNLKDKYFGWFSNYLVNQRAKTEPNYHSLYARLINSIGSTLLHDYVLNATFKQLYLLLSTKELQFNEKNFLKNLGAWLGSITVALNKPIRYNNIAFRELLLDAHRAERLEIIVPFVCRVLQQASSSKVFLPPNPWTLGILKVLLELNQKANWKLNLTFEVEVLFKSLKLKLTDVEPTNYLDNADSIAFISGDLFRLTAEQQQAEQQQIVTVMQQYQQQAVLLHNRQQRKAQQPQPPQSQQPQQSQTQQRASSTGFSGLPSGLDQPVSTGDSQANENPFNTLLGNTLFVTHPDLKRVFQMAIAKSVREILVPAVDKSSSIAVITTVNVVLKDFATEVDEMKLKTAAITMVRHLAQSLARATSIELLKDTIRSTTQSLAPNLMNVPNSPIEELNVAINDNISIALALIEKATMDKATQYVGEQLMQAVAIRRYHKERRSGQLFLAQNANSFSLNLPEPLGLKPTGVTPQQFRIYEEFGKNNIFDTVSSASGGGFSAEGGPVSQQQILGQQLLQQQQRQQQQQQQEQHQQQRQQLLEHEQQKQQQQQQLQLQQQQQLQQQQLQQQQQLQQQQQQQQQQQQQQQQQQLLLQQLQQPHAQSQPGTPQAQTQPSVQVELEQSHRVLVLLMDSLVAQIKENADKALDSLGQENGIKDILFQILTLISRSNQKDQLALKVSQAVVNSMFATSESPLCREVLSLLLEKLCSLSIVARKDVVWWLVYALDSRKFNVPVIRSLLEVNLIQSSKLDTVLVVAIKNNMEGALQFAVNLLRDVILSEKPLFMRSDFVQTLTYLRTVDCDITNSFFQDFEKANILPVNEVRSATNKERMLLVFTEWVKLLQRVPSDDVKVAVFISQMIEKGVLSETNNVIEFTKAALELSVGSFKESDPTSEVFTATDAFSKLIVNLLVLQDFSEVDRSSYFKLIFSVITFVFSEDQGKTENTFNERPYFRLFSSLLCDWEDISSHNFVKVEDQRCRAQLVEFNKEFYRVIASFLHAYQPIAFPGFSFAWITLISHRMFLPKMLKLPEKAGWSQLVLLFTDLLKFMAQYTKKQDVPDAVSVVYKGALRVFLAVANDAPEFLVENHYELLNNLPHVYMQLRNVVLSAFPKHVSIPNPYNPKLSIDTVKICDQPPNVFYDPVHDLKTLKKPVDNYLRIPSASLSRTISSNVFRSEYEKDNGIGFDTVSVDVKLINAIVLHVGIEAALEKQRTTANAVFNVKSSYYTLLADLLNDGTTEVRFHVVQAIANQLRYPNAHTQWFNYVLKNFFNSEEWGENKTEIQEIIVRTLLERIVTNKPHCWGIIVTFTDLLKSTECSLMDLPFVKDVPEVELIIKHLSKYIVKTEETTNEQQNERAPAEVASRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5DC52</accession>
    <name>C5DC52_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">D-serine dehydratase</fullName>
            <ecNumber evidence="11">4.3.1.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">D-serine deaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0A07986g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the conversion of D-serine to pyruvate and ammonia. May play a role in D-serine detoxification.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>D-serine = pyruvate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:13977"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:35247"/>
            <dbReference type="EC" id="4.3.1.18"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:13978"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DSD1 family.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.18" evidence="11"/>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551801.1">
        <property type="nucleotide sequence ID" value="XM_002551755.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DC52"/>
    <dbReference type="FunCoup" id="C5DC52">
        <property type="interactions" value="49"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DC52"/>
    <dbReference type="GeneID" id="8290614"/>
    <dbReference type="KEGG" id="lth:KLTH0A07986g"/>
    <dbReference type="eggNOG" id="ENOG502QRZ0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031639_0_0_1"/>
    <dbReference type="InParanoid" id="C5DC52"/>
    <dbReference type="OMA" id="NCEKMLA"/>
    <dbReference type="OrthoDB" id="20198at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008721">
        <property type="term" value="F:D-serine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036088">
        <property type="term" value="P:D-serine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009636">
        <property type="term" value="P:response to toxic substance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06817">
        <property type="entry name" value="PLPDE_III_DSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.10:FF:000016">
        <property type="entry name" value="D-serine dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="entry name" value="Alanine racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.37.20">
        <property type="entry name" value="D-serine dehydratase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001608">
        <property type="entry name" value="Ala_racemase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051466">
        <property type="entry name" value="D-amino_acid_metab_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026956">
        <property type="entry name" value="D-ser_dehydrat-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042208">
        <property type="entry name" value="D-ser_dehydrat-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28004:SF2">
        <property type="entry name" value="D-SERINE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28004">
        <property type="entry name" value="ZGC:162816-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01168">
        <property type="entry name" value="Ala_racemase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14031">
        <property type="entry name" value="D-ser_dehydrat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01119">
        <property type="entry name" value="D-ser_dehydrat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="PLP-binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0216">Detoxification</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="D-serine dehydratase-like" evidence="14">
        <location>
            <begin position="297"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00051198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00055764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00066349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00069616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00075219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR21359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="415" mass="46112" checksum="1D537AFF578B8725" modified="2009-07-28" version="1">MRYPYQYTALSKKAELLEAFKGKKLSELPTPSFIVDRGKFKENCEKMLANVDRLNVDFRAHVKTHKTVEGTLLQLGSENIKTKKIVVSTLMEAWGLLPLIEKGLIIDVLFSLPVVKSRLPELADLGARIPNLRLMVDNGEQFEVLAEFSKKHKIQKKWSLFIKVNMGSDRAGFDESSTLDEALFDLLQSPAKDYLSLYGFYCHAGHSYASSTEEQARHFLLNEIDSGNNACKKALNIDPSLKLQISVGATPTAHASQSLDISSIGELYGKLELHAGNYPFCDLQQVSTHCVGLDQVSCRVLAEVLSSYPQRGSRGPGEQLINAGVIALAREFGSLPGYGKIVSPKGYENWIVGRLSQEHGILTCLQDTETKMIPLGTKIGIVPQHSCITAASYPWYFVVEHGDIVVDIWVPWRGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>C5DC66</accession>
    <name>C5DC66_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">KLTH0B00396p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0B00396g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551815.1">
        <property type="nucleotide sequence ID" value="XM_002551769.1"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DC66"/>
    <dbReference type="GeneID" id="8290634"/>
    <dbReference type="KEGG" id="lth:KLTH0B00396g"/>
    <dbReference type="eggNOG" id="KOG0054">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_27_1_1"/>
    <dbReference type="InParanoid" id="C5DC66"/>
    <dbReference type="OMA" id="NPWMING"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18596">
        <property type="entry name" value="ABC_6TM_VMR1_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18604">
        <property type="entry name" value="ABC_6TM_VMR1_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000630">
        <property type="entry name" value="ATP-binding cassette (ABC) transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000010">
        <property type="entry name" value="Multidrug resistance-associated ABC transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000973">
        <property type="entry name" value="Multidrug resistance-associated protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF353">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN_PERMEASE VMR1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="16"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="109"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="179"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="312"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="353"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="462"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="549"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="960"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1034"/>
            <end position="1056"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1062"/>
            <end position="1081"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="12">
        <location>
            <begin position="324"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="637"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="12">
        <location>
            <begin position="927"/>
            <end position="1202"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="1238"/>
            <end position="1474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR21376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1507" mass="168237" checksum="8FE0D9B525FA01DE" modified="2009-07-28" version="1">MMQFATETEKLRSTVAFLYSFLVPLSGGSFVLLGWDLLRLYWASQRNACGYEAPEADASTSLISRKPEHQITEHASSYPQLEWRVLCESSQQDCEQVVKKVFGKELLKSYAELLTIILCIVLELSLQRAVNRTQEEAPWILSSQIFWFSLLVSSLCRIVSLHRGGKSFITFQKLRALNFLLFGFIFCVKVLHFTLVFLRSQVKSLEGKYVTGGTFLAGVLSILALSSQQSFGLTDPPLNQNDAPPAPEGSSSIFHSLTFSWLDGLIFRRSQKQLRFQDIFDLQPKYRTLPVIKRYSDRSHGCTSLIKRLTAFCWKSIIMQCTWSLAGAIASFMPTLQMKKILEYIENPSGSNIRVAWVCVFVMIVSKIIAALCESQAVYLGARVCFQLKSIITAEISRKALKQSLFSSESKTRCGATNGEVINLVAIDSTAVSELCGSLHYLVQAGIMTTVAISLLYQMLGWSAFVGVSVIIILLPFNVKIATLMQTYQSEALAMTDKKTNAISAMLGSIKNVKFLCWENHFQELIMGFRSSECSFLTKKLFCWATLHLVWFLTPTLVTAATFGASIYLQKQKITVASAYTALTLFALLRNPLTQLATMLGTIIQTTVSIQRIEKFLNRSNTKKHEILKDSFTEMGFRNASLTWNRFDTGFMLKNLTIDFELHKLNVIIGPSGSGKSSVLLALLGELDLIEGSIRVPRGYMHDDRCSFAFCSQTPWITNATVKENIVFSSQFNTKRYQEVLHACGLEEDILEMQEGDETVVGDKGMTLSGGQRQRLALARAVYSGAEHLLLDDCLSAVDPGMAVWIFQKCIMGPLMKGKTCILATHNIALVQKHADMIILLDNGRVSKIGKARDFNFDSEFDYSKPSKLEKAEMKPNKKPKKEMILQPDTGFSREEHKEDGSIKIEVYNWLVKHLGGWRTITAFTMLLCLTHGIEMTQNLWIKIWCSSGDGANEHYKSSIYYLSTYAAIGLFHASVTTLIMLKFLCIGLEASSKVFAEILNRVLHAKMTFFDFTPAGRILNRITRDFEILEQELMVLLLQLLFAITSLGAILLLISVIATKFLIFAFLLGIIYAVMITSYIPAFRDLKRYEGITRSPIFEQFSELLSGFVTIRGFGKEKSCMGQLFRKLDLNNRPAYYVSVVQLWLCLRVEVLGSLVLGATATLSLLNAKELGAGLAGLALTYAMPFSQSALWTATVSSNVEIKMTSVERIKEYTEIDCEDTKAGALINTQWSGGAEISFDHVYVRYAPHLPDVLKNVSFTLLPNSKVAIVGRTGSGKSTVMMSLFRLIELTNGTIRIGGIDIASLNLQTLRRELTVIPQDPSLFSGTIKSNIDPFGRYSDQELTHFARRIGLFSGTDDVRTFNSLGSLVSEGGENMSQGQRQLLCLARALLQKSKILILDEATASIDHETDATIQQVVRDFSSGGIVITVAHRMSTIIDYDQVIVLDSGIVKENNHPYLLLKQNGAFHQMCKESGEYERLEDAAKKAYQKGLSQARSAPRNSESKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>C5DC86</accession>
    <name>C5DC86_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0B00990p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0B00990g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Bud neck</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551835.1">
        <property type="nucleotide sequence ID" value="XM_002551789.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DC86"/>
    <dbReference type="FunCoup" id="C5DC86">
        <property type="interactions" value="206"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DC86"/>
    <dbReference type="GeneID" id="8290661"/>
    <dbReference type="KEGG" id="lth:KLTH0B00990g"/>
    <dbReference type="eggNOG" id="KOG2655">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017718_7_4_1"/>
    <dbReference type="InParanoid" id="C5DC86"/>
    <dbReference type="OMA" id="GYDSAMN"/>
    <dbReference type="OrthoDB" id="416553at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031105">
        <property type="term" value="C:septin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01850">
        <property type="entry name" value="CDC_Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001557">
        <property type="entry name" value="Septin homolog spn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016491">
        <property type="entry name" value="Septin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006698">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="7">
        <location>
            <begin position="19"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="296"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="344"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="297"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="329"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR21397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="404" mass="46192" checksum="E541BAC41E2D988E" modified="2009-07-28" version="1">MASIIEASAALRKRKNLKRGINFTLMVVGESGSGRSTFVNTLCGQQVVDVSTSIMLPTDRSTQIDLQLREETVELEDDEGVKIQLNIIDTPGFGDSLDNSQSFNVVSDYIRHQYDEILLEESRVRRNPRFKDGRVHCCLYLINPTGHGLKETDVEFMKTLGPLVNVIPVISKADSLTPAELKLNKKLIMEDIDYYHLPIYNFPFEENDISDEDYETNMYLRSLVPFSVIGSNEVYDLGDGAMIRGRQYPWGTLDVEDSSLSDFVVLRNTLLISHLHDLKDYTHEILYERYRTEALSGETSSQRANGNSSTAYADSPVASGKSFQHAETDSLNSSKQASANQDTYLAREEQIRLEEERLRAFEERVQQELLMKRQELLQREQELREIEERIEREARIKEESGDLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCF3</accession>
    <name>C5DCF3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0B02596p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0B02596g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551902.1">
        <property type="nucleotide sequence ID" value="XM_002551856.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCF3"/>
    <dbReference type="GeneID" id="8290733"/>
    <dbReference type="KEGG" id="lth:KLTH0B02596g"/>
    <dbReference type="HOGENOM" id="CLU_1845450_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCF3"/>
    <dbReference type="OrthoDB" id="4034580at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR21464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="147" mass="16142" checksum="D95344D3E2A49473" modified="2009-07-28" version="1">MSILVVDMDEVRSLAQRHSIEMGLERLRVVGQYVGSWWDAGRLRLEFGNVSGLASAGGRAVLDASESALERVRRARAGLVEGAVCDVRCCVMGRDVEVMELRVLTLREVQALGEFLAGDAGREFMSLSSGALQHAGHDRRDDEDRAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCF4</accession>
    <name>C5DCF4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribosome biogenesis protein RLP24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0B02618g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL24 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551903.1">
        <property type="nucleotide sequence ID" value="XM_002551857.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCF4"/>
    <dbReference type="FunCoup" id="C5DCF4">
        <property type="interactions" value="1085"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCF4"/>
    <dbReference type="GeneID" id="8290734"/>
    <dbReference type="KEGG" id="lth:KLTH0B02618g"/>
    <dbReference type="eggNOG" id="KOG1723">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089419_1_1_1"/>
    <dbReference type="InParanoid" id="C5DCF4"/>
    <dbReference type="OMA" id="NAGKEMT"/>
    <dbReference type="OrthoDB" id="10262490at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902626">
        <property type="term" value="P:assembly of large subunit precursor of preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00472">
        <property type="entry name" value="Ribosomal_L24e_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.170.20:FF:000001">
        <property type="entry name" value="probable ribosome biogenesis protein RLP24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.170.20">
        <property type="entry name" value="Ribosomal protein L24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038630">
        <property type="entry name" value="L24e/L24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056366">
        <property type="entry name" value="Ribosomal_eL24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000988">
        <property type="entry name" value="Ribosomal_eL24-rel_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011017">
        <property type="entry name" value="TRASH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10792">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10792:SF8">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN RLP24-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01246">
        <property type="entry name" value="Ribosomal_L24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00746">
        <property type="entry name" value="TRASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="TRASH" evidence="5">
        <location>
            <begin position="6"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="150"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="155"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR21465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="199" mass="23561" checksum="90DB078F8517EF2A" modified="2009-07-28" version="1">MRVYSCHFCSSPCYPGHGIMFVRNDAKEFRFCRSKCHKNFKQRRNPRKLRWTKAFRKAAGKELAVDSTLAFAQRRNVPVRYNRELVATTLRAMARVEEIRQKRERAFYKNRMRGNKEREFLRDRQLVEKNPELLNMRDVELLRTAERAERVEAASDAESEEMDVDSESEHESEDESEAEAQKQKVVLTNKRKVGKKIAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCI4</accession>
    <name>C5DCI4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Adenylate cyclase</fullName>
            <ecNumber evidence="5">4.6.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">ATP pyrophosphate-lyase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Adenylyl cyclase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ordered locus">KLTH0B03322g</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Plays essential roles in regulation of cellular metabolism by catalyzing the synthesis of a second messenger, cAMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP = 3',5'-cyclic AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15389"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <dbReference type="EC" id="4.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the adenylyl cyclase class-3 family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.1" evidence="5"/>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551933.1">
        <property type="nucleotide sequence ID" value="XM_002551887.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DCI4">
        <property type="interactions" value="589"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCI4"/>
    <dbReference type="GeneID" id="8290766"/>
    <dbReference type="KEGG" id="lth:KLTH0B03322g"/>
    <dbReference type="eggNOG" id="KOG0618">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000430_3_0_1"/>
    <dbReference type="InParanoid" id="C5DCI4"/>
    <dbReference type="OMA" id="CLICLYD"/>
    <dbReference type="OrthoDB" id="2021138at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006171">
        <property type="term" value="P:cAMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01775">
        <property type="entry name" value="RA_PHLPP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1230:FF:000084">
        <property type="entry name" value="Adenylate cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048580">
        <property type="entry name" value="CYAA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000159">
        <property type="entry name" value="RA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055071">
        <property type="entry name" value="RA_PHLPP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF1">
        <property type="entry name" value="RAS SUPPRESSOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21187">
        <property type="entry name" value="CYAA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23010">
        <property type="entry name" value="RA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00314">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50200">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0115">cAMP biosynthesis</keyword>
    <keyword evidence="7" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="14" id="KW-0456">Lyase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Ras-associating" evidence="19">
        <location>
            <begin position="540"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="domain" description="PPM-type phosphatase" evidence="20">
        <location>
            <begin position="1221"/>
            <end position="1487"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="18">
        <location>
            <begin position="1531"/>
            <end position="1668"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="94"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="317"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="383"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="17">
        <location>
            <begin position="55"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="121"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="139"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="166"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="220"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="264"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="285"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="323"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="391"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="460"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00021420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00032637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="CAR21495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1890" mass="209441" checksum="E02D25662BB8C68A" modified="2009-07-28" version="1">MSEQTRRRANSLEAEKSFQVPEDAAEAGASGGLHGRPHFRKQYSVGGFGPTFSGGASGASGGIAGAGSASAQGHGARGRAAKALGKGRYKGFHNPVALHSPDDPIPPYAHPIKPRIPGLNRTISGNSLFSSSQAARDPQQLQHQQQQQQRKGSRAGSLFKRLTGMKMTNASASEPSDQESAAANPTAGPAGIRRKMSTLIHGGSGAGSGQAQDAGHVSGSDLNSKTSKSGFLNTPSSTNSRRGSAASIITSPSGNNSPKISHISNSNVNNALSATSASASANDSGNQVSDSSESPGRNALNRSTSYFLLDTDLNNLSDITNISDKKPKEKAKLPPKSRSQTPVAKVQPQDIGKAPLVDTETSAPHEAAHSKVQWIAPESWDIDEEVGKPLPGKKNKRRVHRHHTSNTTSKIDRERAGGATKKPGRPKQKENTPKLGSLKEVTDQEHNHLSTTAQEDTKESSGSAASISSAASRSQEFSEAASSTSDYEDDYGDESHWRAGSLDSLQTYDSDQAKNDEAFDRVGYELDKYYNDFSDIDPTRRYAIRVFNTDETFTTLSLTPNTTVQEMIPQLKRKFNIGQGNYQVSLKVAKILKVLRPTARPILIQRKLLLLNGYQKGDPLHILGIEDLSYVFNFIFHPVATSHLSYEEEQRLTRGDFIHVDLRSMNLTTPPIIFYQYTPNIESLDVSNNANIFLPLDFIESASKLSSLRMVNIRASRFPPNITEAHVLVSLDLERNFIERIPPSISNLSELTILNLQCNELDRLPTGFTELKNLRLLDISSNNFAQYPEVINHCTNLLQVDLSYNKIQTLPDSINRLTKLAKMNLSNNKLTNIGSLSGMVNLRTLNLKDNRITSLKLENSNLQNLSISNNRISVFDDKLPKLKSLEIHENPITSISYDGEYLVNIVTLSLNKAKLASLPPELLCKLPRLEKLELNGNNLTQIPPEISKLTKLVYLSAARNKLESISEDITELRNLKTLDIHSNNLSNLVKGLGKMELINFNVSSNLLGNTMDIQEVFLNRRDSVLAKSLRFLSMADNNLGDQFWTLFNMYENLKTLNLSYNNLSDLSNFKLENLTELYLSGNKFITLQGDTVLRWKSLKVLMLNGNNLLSLPSELSQLTQLNVLDVGSNQLKYNISNYHYDWNWNNNKNLKYLNFSGNKRFEIKSTAALDSESKADLSDLTILKELRILGLMDVTLKTSKVPDEGVNMRLRTTASVLNGMKYGVADTLGLKDSVTSRDVTFERFRGKEDECLVCLFDGKNENVSTGHNISKIIRDIYHKILIRQLEKYGDTAEGIKNALRFSFLQLNKEINSTLNSVDNEATSEDFSSADLLSGSSATVLYMKGKRLYTANIGDTMAILSKNNGDVVPLTKLHVPSKREEYERIRISGGYVNNYKLDGVSDVSRAVGFFDLLPHIHASPDLSEFNLTTTDDLVIIATAKLWDYLDYQTACDICRENKVEPMLAAEKLKDYAISFGCAANMTILCISLDKSSSNQNNFMLNKNDLISRRSTFEDSTLRRLQPEISPPTGNLAMVFTDIKNSTFLWESFPNAMRSAIKTHNDIMRRNLRIFGGYEVKTEGDAFMVAFPTPISALVWCMSVQLKLLEAEWPEEITSIQGGCLITDNEDRKIYLGLSVRMGIHWGCPVPELDVVTQRMDYLGPVVNKASRVSGIADGGQITLSSDFCSEFNKILGLHERVVKENAPLKEVYGEEFVGEIMEREVHLLESIGWEFRELGEKKLKGLETKEFITIAYPKALEARHGFTTRDAETSLVDEETLFQLRAVSTKLESLVSVLNGVMLEKKEYSEAGSYVNLDKKQISAVSHSTTEGDRIALLDHIITRLESIVATLQIRQRVEGALEPPKIGDAGGERSIFEIVDQLLLETGRLTDSRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCK0</accession>
    <name>C5DCK0_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 12">Complex III subunit 9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0B03674g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The complex plays an important role in the uptake of multiple carbon sources present in different host niches.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII), a multisubunit enzyme composed of 3 respiratory subunits cytochrome b, cytochrome c1 and Rieske protein, 2 core protein subunits, and additional low-molecular weight protein subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Mitochondrion inner membrane</location>
            <topology evidence="1 12">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the UQCR10/QCR9 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551949.1">
        <property type="nucleotide sequence ID" value="XM_002551903.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCK0"/>
    <dbReference type="FunCoup" id="C5DCK0">
        <property type="interactions" value="346"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCK0"/>
    <dbReference type="GeneID" id="8290782"/>
    <dbReference type="KEGG" id="lth:KLTH0B03674g"/>
    <dbReference type="eggNOG" id="KOG3494">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_171977_1_0_1"/>
    <dbReference type="InParanoid" id="C5DCK0"/>
    <dbReference type="OMA" id="IKHKYEV"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045275">
        <property type="term" value="C:respiratory chain complex III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006122">
        <property type="term" value="P:mitochondrial electron transport, ubiquinol to cytochrome c"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.260:FF:000001">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.260">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008027">
        <property type="entry name" value="QCR9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036656">
        <property type="entry name" value="QCR9_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12980:SF0">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12980">
        <property type="entry name" value="UBIQUINOL-CYTOCHROME C REDUCTASE COMPLEX, SUBUNIT X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05365">
        <property type="entry name" value="UCR_UQCRX_QCR9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81514">
        <property type="entry name" value="Subunit X (non-heme 7 kDa protein) of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0249">Electron transport</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 12" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="16"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU368056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR21511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="71" mass="8095" checksum="69DBB496EB2C16C1" modified="2009-07-28" version="1">MAFSTIYNIFFKRNSIFVGTVFASSFVFQAAFDSAVTSWYEQHNKGKLWKDVKAKLAQGGDDDDEDDEDDE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2010-10-05" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCN3</accession>
    <name>ARGJ_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Arginine biosynthesis bifunctional protein ArgJ, mitochondrial</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="1">Glutamate N-acetyltransferase</fullName>
                <shortName evidence="1">GAT</shortName>
                <ecNumber evidence="1">2.3.1.35</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="1">Ornithine acetyltransferase</fullName>
                <shortName evidence="1">OATase</shortName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="1">Ornithine transacetylase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="1">Amino-acid acetyltransferase</fullName>
                <ecNumber evidence="1">2.3.1.1</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="1">N-acetylglutamate synthase</fullName>
                <shortName evidence="1">AGS</shortName>
            </alternativeName>
        </domain>
        <component>
            <recommendedName>
                <fullName evidence="1">Arginine biosynthesis bifunctional protein ArgJ alpha chain</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="1">Arginine biosynthesis bifunctional protein ArgJ beta chain</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name type="ordered locus">KLTH0B04488g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of acetylglutamate from glutamate and acetyl-CoA, and of ornithine by transacetylation between acetylornithine and glutamate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>N(2)-acetyl-L-ornithine + L-glutamate = N-acetyl-L-glutamate + L-ornithine</text>
            <dbReference type="Rhea" id="RHEA:15349"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:44337"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:57805"/>
            <dbReference type="EC" id="2.3.1.35"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-glutamate + acetyl-CoA = N-acetyl-L-glutamate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24292"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:44337"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.1.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; L-ornithine and N-acetyl-L-glutamate from L-glutamate and N(2)-acetyl-L-ornithine (cyclic): step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of an alpha and a beta chain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="1">The alpha and beta chains are autoproteolytically processed from a single precursor protein within the mitochondrion.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">This protein may be expected to contain an N-terminal transit peptide but none has been predicted.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ArgJ family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.35" evidence="1"/>
    <dbReference type="EC" id="2.3.1.1" evidence="1"/>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551982.1">
        <property type="nucleotide sequence ID" value="XM_002551936.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCN3"/>
    <dbReference type="SMR" id="C5DCN3"/>
    <dbReference type="FunCoup" id="C5DCN3">
        <property type="interactions" value="329"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCN3"/>
    <dbReference type="MEROPS" id="T05.001"/>
    <dbReference type="GeneID" id="8290819"/>
    <dbReference type="KEGG" id="lth:KLTH0B04488g"/>
    <dbReference type="eggNOG" id="KOG2786">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027172_1_0_1"/>
    <dbReference type="InParanoid" id="C5DCN3"/>
    <dbReference type="OMA" id="WGRIVMA"/>
    <dbReference type="OrthoDB" id="2017946at2759"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00106"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00111"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004042">
        <property type="term" value="F:L-glutamate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004358">
        <property type="term" value="F:L-glutamate N-acetyltransferase activity, acting on acetyl-L-ornithine as donor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006592">
        <property type="term" value="P:ornithine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02152">
        <property type="entry name" value="OAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.70.12:FF:000001">
        <property type="entry name" value="Arginine biosynthesis bifunctional protein ArgJ, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.340:FF:000002">
        <property type="entry name" value="Arginine biosynthesis bifunctional protein ArgJ, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2330.10:FF:000001">
        <property type="entry name" value="Arginine biosynthesis bifunctional protein ArgJ, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2330.10">
        <property type="entry name" value="arginine biosynthesis bifunctional protein suprefamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.340">
        <property type="entry name" value="ArgJ beta chain, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.70.12">
        <property type="entry name" value="L-amino peptidase D-ALA esterase/amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01106">
        <property type="entry name" value="ArgJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002813">
        <property type="entry name" value="Arg_biosynth_ArgJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016117">
        <property type="entry name" value="ArgJ-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042195">
        <property type="entry name" value="ArgJ_beta_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00120">
        <property type="entry name" value="ArgJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003802">
        <property type="entry name" value="PRK05388.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23100">
        <property type="entry name" value="ARGININE BIOSYNTHESIS BIFUNCTIONAL PROTEIN ARGJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23100:SF0">
        <property type="entry name" value="ARGININE BIOSYNTHESIS BIFUNCTIONAL PROTEIN ARGJ, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01960">
        <property type="entry name" value="ArgJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56266">
        <property type="entry name" value="DmpA/ArgJ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0012">Acyltransferase</keyword>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0055">Arginine biosynthesis</keyword>
    <keyword id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword id="KW-0496">Mitochondrion</keyword>
    <keyword id="KW-0511">Multifunctional enzyme</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000398058" description="Arginine biosynthesis bifunctional protein ArgJ alpha chain" evidence="1">
        <location>
            <begin position="1"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000398059" description="Arginine biosynthesis bifunctional protein ArgJ beta chain" evidence="1">
        <location>
            <begin position="215"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="215"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="301"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="436"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="441"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole" evidence="1">
        <location>
            <position position="136"/>
        </location>
    </feature>
    <feature type="site" description="Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole" evidence="1">
        <location>
            <position position="137"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by autolysis" evidence="1">
        <location>
            <begin position="214"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03124"/>
        </source>
    </evidence>
    <sequence length="441" mass="47232" checksum="D6A3DFFFBDFBB26B" modified="2009-07-28" version="1" precursor="true">MRVSSALLQKAAKTVDKYSLYVPKSGVFPKGFKVASLASGVKKNGNRDLGIILNTNKSRPSNAAAVFTTNRFKAAPVLVSKQVLDGSSGEGVNAIVANSGCANAVTGELGMQDAQKVAGQVNAHIGKENSTLVMSTGVIGQRLQMDKISKGINTLFDQKQFGSDFNSWLNLAKSICTTDTFPKLISSKFQLSDGTEYTLTGISKGAGMICPNMATLLGFIVTDLPIKASTLQAMLTAAVNRSFNCISVDGDMSTNDTICMLANGAVDTFVIDEASPDFEQVKSQVTEFAKQLAQLVVRDGEGATKFVTVNVKNSANFKDARIIAETISNSMLVKSALYGQDANWGRILCAIGYSKLENLASLDESKINVSFIATDNSSPRELKLIANGVPQFDIDENRASEILALPDLEILVDMGTGSEECQFWTCDLSHEYVTINGDYRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCP7</accession>
    <name>C5DCP7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0B04796p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0B04796g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551996.1">
        <property type="nucleotide sequence ID" value="XM_002551950.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCP7"/>
    <dbReference type="GeneID" id="8290833"/>
    <dbReference type="KEGG" id="lth:KLTH0B04796g"/>
    <dbReference type="eggNOG" id="ENOG502R0RA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1525423_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCP7"/>
    <dbReference type="OMA" id="EAWGDAK"/>
    <dbReference type="OrthoDB" id="4023585at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1430">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="207" mass="22039" checksum="8DDB60AAB6AC1463" modified="2009-07-28" version="1">MLRSTASHALRIPSRLVATPAVYSSASRVCALHSSARVPKSLGEKVRSTLSDLNKKVGEAAASGIDKAQEASHTVEEKSHTAAEATKHGLEQLNKKTGQAAASGIDKAQNMAQEAGEKAQELKKRDLTQEAKGKAQELKDSDLAQEAKDKAQDLKEGAQEKAGEAKGKAQELKHEGKGKAQELKHEAKGKAHEGAAKAKETAREYKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCU2</accession>
    <name>C5DCU2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA-directed RNA polymerase III subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0B05830g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the RNA polymerase III (Pol III) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the eukaryotic RPC7 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552041.1">
        <property type="nucleotide sequence ID" value="XM_002551995.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCU2"/>
    <dbReference type="FunCoup" id="C5DCU2">
        <property type="interactions" value="98"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCU2"/>
    <dbReference type="GeneID" id="8290880"/>
    <dbReference type="KEGG" id="lth:KLTH0B05830g"/>
    <dbReference type="eggNOG" id="ENOG502RZ0V">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072641_2_0_1"/>
    <dbReference type="InParanoid" id="C5DCU2"/>
    <dbReference type="OMA" id="ADPYENT"/>
    <dbReference type="OrthoDB" id="5377312at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006383">
        <property type="term" value="P:transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024661">
        <property type="entry name" value="RNA_pol_III_Rpc31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15367">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15367:SF2">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11705">
        <property type="entry name" value="RNA_pol_3_Rpc31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000777">
        <property type="entry name" value="RNA_polIII_C31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="169"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="189"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="215"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR000777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR21603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="232" mass="25901" checksum="90B616431B80BB8E" modified="2009-07-28" version="1">MSFQRGGREGNSFAKNLPFGLSYSDVGVNENTELPSIPLPVNGLVTSKERITSLKYMNFIKAVKEGPFYTGSMSLTIDGTGKQKQLVDEDGINDGIERYSDRYLKKRKIGVSIDEHPFHLEFFPDELYQVMGINKKKLLALSNLNRQDDVFTGSAKDDDAEGLSMLEKLKGLAEDEDETANADGKDDAHEAEDLDDDFGDSDEDDDYNAEKYFDDGDDDAENEDDYGDEPAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCV5</accession>
    <name>C5DCV5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0B06116p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0B06116g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the INSIG family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552054.1">
        <property type="nucleotide sequence ID" value="XM_002552008.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCV5"/>
    <dbReference type="FunCoup" id="C5DCV5">
        <property type="interactions" value="28"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCV5"/>
    <dbReference type="GeneID" id="8290893"/>
    <dbReference type="KEGG" id="lth:KLTH0B06116g"/>
    <dbReference type="eggNOG" id="ENOG502QX4Q">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088332_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCV5"/>
    <dbReference type="OMA" id="IEWSSFL"/>
    <dbReference type="OrthoDB" id="205546at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016126">
        <property type="term" value="P:sterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025929">
        <property type="entry name" value="INSIG_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15301">
        <property type="entry name" value="INSULIN-INDUCED GENE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15301:SF3">
        <property type="entry name" value="PROTEIN NSG1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07281">
        <property type="entry name" value="INSIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="132"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="165"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="236"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR21616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="262" mass="29281" checksum="F8A7FBCDC0DDF685" modified="2009-07-28" version="1">MARKKAQMANGNYSVSSESISNLTRPQLYGIYDADITHSEDAEVYEEAKKSVGKFPEDMIKRSGAHGQRSMLQRATHILFSLTFLGVAGISYNELSKQLHDNHELHEEFASRPLALGVEICRTLSFGVVPTWMAYALEGVLFGALLPLMDTLWGSPVRTTTFTSFLRSTNAMLGVAFGIRKIEWSSSLQASGAWFLLNIVLWMFFDGTPSMLLGCLGIGTIASVTSYRDVTEFSQMLYFMDFYFLGLLLFGKLGRYLYGHRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-04-02" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCZ5</accession>
    <name>C5DCZ5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0B07062p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0B07062g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May play a role in mRNA splicing.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of a tri-snRNP complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SNUT3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552094.1">
        <property type="nucleotide sequence ID" value="XM_002552048.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCZ5"/>
    <dbReference type="GeneID" id="8290934"/>
    <dbReference type="KEGG" id="lth:KLTH0B07062g"/>
    <dbReference type="HOGENOM" id="CLU_2292209_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCZ5"/>
    <dbReference type="OrthoDB" id="21368at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013957">
        <property type="entry name" value="SNRNP27"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31077">
        <property type="entry name" value="U4/U6.U5 SMALL NUCLEAR RIBONUCLEOPROTEIN 27 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31077:SF1">
        <property type="entry name" value="U4_U6.U5 SMALL NUCLEAR RIBONUCLEOPROTEIN 27 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08648">
        <property type="entry name" value="SNRNP27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="U4/U6.U5 small nuclear ribonucleoprotein 27kDa protein" evidence="9">
        <location>
            <begin position="48"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="23"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="84"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR21656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="101" mass="10766" checksum="FAE38953678ACA87" modified="2009-07-28" version="1">MSSQSSPSKEASKPAAASPENPSAEKQKVESKPKGLQPASNESQPQPADVASLMGFASFGTTKQKKVQGKGSGGKRVEKTAAKSSRKYRQYVNRKSKKLVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>C5DD11</accession>
    <name>C5DD11_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0B07414p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0B07414g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552110.1">
        <property type="nucleotide sequence ID" value="XM_002552064.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DD11"/>
    <dbReference type="FunCoup" id="C5DD11">
        <property type="interactions" value="33"/>
    </dbReference>
    <dbReference type="GeneID" id="8290950"/>
    <dbReference type="KEGG" id="lth:KLTH0B07414g"/>
    <dbReference type="eggNOG" id="ENOG502S3IE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039198_0_0_1"/>
    <dbReference type="InParanoid" id="C5DD11"/>
    <dbReference type="OMA" id="WSENAEQ"/>
    <dbReference type="OrthoDB" id="5328412at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR21672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="503" mass="57385" checksum="DD5061BCE12D7272" modified="2009-07-28" version="1">MRRNVSKKHVLTTEEDFYVDATSKEEQAERWALSDIKKTIGQYMAAVNAYEQGLHAPDRTEIGSYHIYYNQTRLLLKLHTDFIACDGINILQYVNIRDLGDVSPLFLPLDSIIDRFEFAITKFGDLCSWDLFFNLLTCYLSILEESQEELTGEVLLKIFERFVELAQNLIQLHLRELETWDVSFENKDDDFQSMDLSQQSFDSTTGKGIKEVHDTSTAEYVEMQDEITHLTLVETLSVCFRFVSTLMEILGEAAHGKRATINPIQSNYLGDAALQFTSQLKLFASEIDRSKVGNEIMELDLAMSLVQGLEIAISADYDSLQRFIEESGENLDKLMICIDILRLCLTNQNSTFSQKWSFCSEIGRLLSRVESQLVQKRLDIMSGHLKGLENELSPTVFKLCDVMITRSDNELLRWTMKESESGSSSNQNASKTSEILLKNAGVLLKNATAIAQKPCGFREYVSDKLKRNYIYKQAAERLEFINTGKITGVIEDLVSEHPFFAKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5DD57</accession>
    <name>C5DD57_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0B08470p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0B08470g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552156.1">
        <property type="nucleotide sequence ID" value="XM_002552110.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DD57"/>
    <dbReference type="FunCoup" id="C5DD57">
        <property type="interactions" value="43"/>
    </dbReference>
    <dbReference type="GeneID" id="8290996"/>
    <dbReference type="KEGG" id="lth:KLTH0B08470g"/>
    <dbReference type="eggNOG" id="KOG3378">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035143_1_0_1"/>
    <dbReference type="InParanoid" id="C5DD57"/>
    <dbReference type="OMA" id="EPPHFEL"/>
    <dbReference type="OrthoDB" id="436496at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008941">
        <property type="term" value="F:nitric oxide dioxygenase NAD(P)H activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019825">
        <property type="term" value="F:oxygen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071500">
        <property type="term" value="P:cellular response to nitrosative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046210">
        <property type="term" value="P:nitric oxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01040">
        <property type="entry name" value="Mb-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.490.10">
        <property type="entry name" value="Globins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000971">
        <property type="entry name" value="Globin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009050">
        <property type="entry name" value="Globin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012292">
        <property type="entry name" value="Globin/Proto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044399">
        <property type="entry name" value="Mb-like_M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43396:SF6">
        <property type="entry name" value="ABL201WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43396">
        <property type="entry name" value="FLAVOHEMOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00042">
        <property type="entry name" value="Globin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46458">
        <property type="entry name" value="Globin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01033">
        <property type="entry name" value="GLOBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Globin" evidence="2">
        <location>
            <begin position="190"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="364"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="161"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="373"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="420"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS01033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR21718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="431" mass="47286" checksum="A178DAC463A0D19D" modified="2009-07-28" version="1">MEENRSFYSPLDRSQIAFIPADLESISENDPSVEPSPGLPRLSGDSHIFKESFRDSYVPSVTSQSIFSEAHPPSSLSSVDELDRLSSLSSGSSCGTNMSYAARYKIVLKLTSREIKLIRESWSMMLNDELSGDNLRTFIRKIVHEIGGLPWVRASGRRTRNNSQNTSLSANSSASSPGSGSPPSPKHASAIPTGNTGAFASSIFCQQFYGNLLYMAPSIEAMFPSIRHQAVSFAGVLTMTVNNLDNLSALEAYLCALGKRHARILSIEPPHFELMGLAFLKTIKERFGVHCTLELEETWSRLYSYLANSILQFGIDPVLKIDALKNEMIFPVLDVTKGLPSTKSMFQPLQPSALNAHALATQKLRNNSRPENISPLTKPPISSKKSLPISTSNRNISGSALPQQHKKEQQKPKPLGASALKRDSDKDCVIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDA2</accession>
    <name>C5DDA2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0B09526p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0B09526g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552201.1">
        <property type="nucleotide sequence ID" value="XM_002552155.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDA2"/>
    <dbReference type="FunCoup" id="C5DDA2">
        <property type="interactions" value="54"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDA2"/>
    <dbReference type="GeneID" id="8291044"/>
    <dbReference type="KEGG" id="lth:KLTH0B09526g"/>
    <dbReference type="eggNOG" id="KOG2214">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009031_5_0_1"/>
    <dbReference type="InParanoid" id="C5DDA2"/>
    <dbReference type="OMA" id="GYGNIDQ"/>
    <dbReference type="OrthoDB" id="10049244at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006641">
        <property type="term" value="P:triglyceride metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050301">
        <property type="entry name" value="NTE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021771">
        <property type="entry name" value="Triacylglycerol_lipase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226">
        <property type="entry name" value="NEUROPATHY TARGET ESTERASE/SWISS CHEESE D.MELANOGASTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226:SF44">
        <property type="entry name" value="TRIACYLGLYCEROL LIPASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11815">
        <property type="entry name" value="DUF3336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNPLA" evidence="5">
        <location>
            <begin position="189"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="4">
        <location>
            <begin position="220"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR21763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="580" mass="66143" checksum="A53F21ED14A8A0AF" modified="2009-07-28" version="1">MFKLYILSFFYLFLNHIPPFVWALGHVVSDIFQFWMRRLMNYLRPRSRNLYHGAISDLEQCDNFSDWYQKAAIVDEITGADLWRRNFYSQRYDVNSVLEQYAVLDEALDENDAHTIVHRFSTTGPSMLRNFAGIVDKRLFTKSLIGTKLLIEQYLDKVLECLQFLCQTPGVARTSFFQRCKLSLGTTAFILQGGSLFGLFHLGVIRGLLEQKLLPNIISGSSMGACIGSLCSVLTNEELSVLLSGDNLLNAIKSDLDLLKQCGYGNIDQDLNLGSLLQNVVHRGYSKDVFLFINFVNKVIIKDLTFEEAFQRTGKILNIIVHPTDQNRCPSLLNYVTTPNVLIACAIDCSLGSEVVSSGTKLMCKNLKNEIVDYIPDTGSSRLVFLTPQNASETGLIESPYTRLTELFNVNNFIVSLARPYLAPLVMNDLKHEIKTSKYYYYKHYPTTDMSNFTPLQLSNMNEVEPLGFKFRYHLERKLKHIVTMELRHRVEVMDNLGILSHWIKRLAIDEKTPRSATEITIVPNLKSLSLSRVIEGQLDNIPYWIKCGEQSCWPVLALIKTRCAVEFGLDEIIKSKKNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDB9</accession>
    <name>C5DDB9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0B09922p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0B09922g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552218.1">
        <property type="nucleotide sequence ID" value="XM_002552172.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDB9"/>
    <dbReference type="FunCoup" id="C5DDB9">
        <property type="interactions" value="189"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDB9"/>
    <dbReference type="GeneID" id="8291062"/>
    <dbReference type="KEGG" id="lth:KLTH0B09922g"/>
    <dbReference type="eggNOG" id="KOG3351">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035272_0_1_1"/>
    <dbReference type="InParanoid" id="C5DDB9"/>
    <dbReference type="OMA" id="KDVCGPT"/>
    <dbReference type="OrthoDB" id="330671at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004140">
        <property type="term" value="F:dephospho-CoA kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02164">
        <property type="entry name" value="PPAT_CoAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000089">
        <property type="entry name" value="Bifunctional coenzyme A synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001985">
        <property type="entry name" value="PRK00777.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10695:SF46">
        <property type="entry name" value="BIFUNCTIONAL COENZYME A SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10695">
        <property type="entry name" value="DEPHOSPHO-COA KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="1">
        <location>
            <begin position="139"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="285" mass="32087" checksum="F11470693EEC77AB" modified="2009-07-28" version="1">MPAVAITLSSYKETDQNLLDGVLEDILPLLVKEGSSLDIIIVEKIHSVDYLDYVLGKLYSGTRETLLAEDLYDTPINVLFNQDLSSYVDKKWDLLITDKNEVVQLPHKELRKVRLLKEVNEHERQDSSKDDPLYMVSALGGTFDHLHDGHKILLSAAAFLTVSRLIIGVTDQELLQKKQFREQLEPYETRSGNVRSFLSRIKPSLKVEIVALRDVCGPTGAVPEIESLIVSRETVSGGQIVNNARRSKGLKELKVHVVNVLGGKEEDGWKEKISSTELRRRCSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDE0</accession>
    <name>C5DDE0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0C00220p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0C00220g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552239.1">
        <property type="nucleotide sequence ID" value="XM_002552193.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDE0"/>
    <dbReference type="FunCoup" id="C5DDE0">
        <property type="interactions" value="744"/>
    </dbReference>
    <dbReference type="GeneID" id="8291096"/>
    <dbReference type="KEGG" id="lth:KLTH0C00220g"/>
    <dbReference type="eggNOG" id="ENOG502RYBC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_1_2_1"/>
    <dbReference type="InParanoid" id="C5DDE0"/>
    <dbReference type="OMA" id="FGAPMQR"/>
    <dbReference type="OrthoDB" id="41238at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990189">
        <property type="term" value="F:protein N-terminal-serine acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008999">
        <property type="term" value="F:protein-N-terminal-alanine acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000047">
        <property type="entry name" value="Acetyltransferase, GNAT family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051908">
        <property type="entry name" value="Ribosomal_N-acetyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43441:SF2">
        <property type="entry name" value="FAMILY ACETYLTRANSFERASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_7G00850)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43441">
        <property type="entry name" value="RIBOSOMAL-PROTEIN-SERINE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13302">
        <property type="entry name" value="Acetyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="55"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="247" mass="28401" checksum="D8A6A85A8AB8A09B" modified="2009-07-28" version="1">MVAQNLYGQEIGDPLPNWAGREGPHRTVFEGNYCRLEPLNVQNHGLQLFQAFSNAKDDRMWTYLPVGPFKDYPEYKKIATKFEQDQETVHYAVISKEGNRALGSLSLMRADVSNGTIEIGFVMFSPKLQKTTASTEAQFLLMKHVFDGLGYRRCEWKCDSLNAPSRNCALRLGFTFEGTFRNAAVYKGRSRDTQWFSIIDTEWQDIRKSFELWLKAGNFLEGKQNKKLAAIRNEVKESKDVKKDHVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDM7</accession>
    <name>C5DDM7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0C02266p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0C02266g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552326.1">
        <property type="nucleotide sequence ID" value="XM_002552280.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDM7"/>
    <dbReference type="GeneID" id="8291186"/>
    <dbReference type="KEGG" id="lth:KLTH0C02266g"/>
    <dbReference type="eggNOG" id="ENOG502QR7D">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023537_1_1_1"/>
    <dbReference type="InParanoid" id="C5DDM7"/>
    <dbReference type="OMA" id="WFAWCNS"/>
    <dbReference type="OrthoDB" id="7771656at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008313">
        <property type="entry name" value="GH125"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31047">
        <property type="entry name" value="MEIOTICALLY UP-REGULATED GENE 157 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31047:SF0">
        <property type="entry name" value="MEIOTICALLY UP-REGULATED GENE 157 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06824">
        <property type="entry name" value="Glyco_hydro_125"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01149">
        <property type="entry name" value="DUF1237"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002947787" evidence="1">
        <location>
            <begin position="28"/>
            <end position="603"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="603" mass="68256" checksum="EBC30B760C2AE3D5" modified="2009-07-28" version="1">MRRVSSRFIPILASLLFVCIIYNLSTSNQGYLAEPLNEKTPRKPATRPFQGASTAQRYQARVRKCPNYADYASVPHYRDEVSATPLDLPFQRPPENCRTFTSELVEKFIRQFTSRFRDRDLARLFENSFPNTLDTTILWHVSGSSNRKLRNHKEKSALFRNEFPETFVVTGDIHAEWLRDSAWQLSVYQPFIKYDGKLAELIRGAINTQAQLVINNPFCNAFHPPSYCSVKRGRSSIDNVDPKPNWSQVFECKYEVDSLASFLTLSRQYYENAPQELKFSFVTPDWISAVEKLMIVVSVESDSTFDEDGFVNTFPYVFRRNTNVASETLPLAGTGNPVNSGIGLVRSAFRPSDDSTIFQYFIPGNAHLSVELETLAEYLRGYLKQTTSSPSELERVKELISAAAVTSSRIRDGIMDHAIVKHPKFGKVFAYEIDGFGSSLLMDDANIPSLLSLPDLGFLDVDDEIYQNTRRMITCKEGNPYYIKGTHFEGIGGPHIGIHNAWPMSLLVAIRTSENDKEIERLLDMVLQSTGGLGLIHESVQAFKEGGSTYTRPWFAWANSEFAKTILQLAQTKPYLIFRDEYAAEGFTLQNFLLSLPQQAGSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDR6</accession>
    <name>C5DDR6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Acetyltransferase</fullName>
            <ecNumber evidence="5">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0C03212g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="5"/>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552365.1">
        <property type="nucleotide sequence ID" value="XM_002552319.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDR6"/>
    <dbReference type="FunCoup" id="C5DDR6">
        <property type="interactions" value="33"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDR6"/>
    <dbReference type="GeneID" id="8291229"/>
    <dbReference type="KEGG" id="lth:KLTH0C03212g"/>
    <dbReference type="eggNOG" id="KOG4750">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051638_3_0_1"/>
    <dbReference type="InParanoid" id="C5DDR6"/>
    <dbReference type="OMA" id="PFRNMPD"/>
    <dbReference type="OrthoDB" id="25818at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008870">
        <property type="term" value="F:galactoside O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03357">
        <property type="entry name" value="LbH_MAT_GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.10.10:FF:000008">
        <property type="entry name" value="Maltose O-acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039369">
        <property type="entry name" value="LacA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024688">
        <property type="entry name" value="Mac_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43017:SF1">
        <property type="entry name" value="ACETYLTRANSFERASE YJL218W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43017">
        <property type="entry name" value="GALACTOSIDE O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12464">
        <property type="entry name" value="Mac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01266">
        <property type="entry name" value="Mac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Maltose/galactoside acetyltransferase" evidence="6">
        <location>
            <begin position="4"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU367021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR21927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="198" mass="21667" checksum="DE68955463C3541C" modified="2009-07-28" version="1">MEALQKIVPGELYDANYDPQILEIRRQAKIKIHEINHLSPAEGEKRESMIRELFGSVGKNLVIESPFYCDYGPNIHIGDDVAINHNLVILDGAEVRIGNSVFIAPNVGLYTAGHPIDVERRSKGLEFASPIIIEDYVWIGGGVSIVPGVTIKKGSVIAAGSVVIRDIPEGVVAGGNPCKVIRKITDEDKKTTNFIKRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDT3</accession>
    <name>C5DDT3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0C03608p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0C03608g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CCR4/nocturin family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552382.1">
        <property type="nucleotide sequence ID" value="XM_002552336.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDT3"/>
    <dbReference type="FunCoup" id="C5DDT3">
        <property type="interactions" value="513"/>
    </dbReference>
    <dbReference type="GeneID" id="8291246"/>
    <dbReference type="KEGG" id="lth:KLTH0C03608g"/>
    <dbReference type="eggNOG" id="KOG2338">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034867_0_0_1"/>
    <dbReference type="InParanoid" id="C5DDT3"/>
    <dbReference type="OMA" id="PEISNWA"/>
    <dbReference type="OrthoDB" id="428734at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050410">
        <property type="entry name" value="CCR4/nocturin_mRNA_transcr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121">
        <property type="entry name" value="CARBON CATABOLITE REPRESSOR PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121:SF45">
        <property type="entry name" value="NOCTURNIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="4">
        <location>
            <begin position="99"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="336"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="15"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="34"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="350"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR21944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="495" mass="57046" checksum="F907E4CDA55613B4" modified="2009-07-28" version="1">MEEERLADKVNTQIKQDKERHSGEKGHKSKKKGEKPPKVSPEHIAEVRAQREAAKKKKREDMIARGLDPDFPPDLQFIHRPMLSLNPSENANGFVFKLMTYNCLAQALIRRKLFPTSGNALKWFKRSRVLLNEFMHYNSDVLCLQEVDYIQYQSFWKEEFEKLGYESQFHRHGTKNHGIAIVWKRDMFTMVDKMLIDYDKEPSGALEPRTTTKNVGLAIALGFTKKVLAKYPGSSKRGIIVGTTHLFWHPFGTFERTRQCYVMLNKMKEFMHRINVLQDKGATQENLWYPFFCGDFNSQPFDTPYLSVSSKPVTYKGRAKTVIECSTSYTFSKARDGEECDDEEGGNIEKFGDGQPEHPVPEKFSATEEQKGLVESMASLHNSLDMRGISIYSVGYKHVHAENAGLDNNFNEPEISNWAETWRGLLDYIFFIKKWDFSDKASPEELEAFQDENGIKIRGLLRMPPAKEMPNHGQPHEGEYGSDHLSMMCELELKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-04-02" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDZ0</accession>
    <name>C5DDZ0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0C04928p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0C04928g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552439.1">
        <property type="nucleotide sequence ID" value="XM_002552393.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDZ0"/>
    <dbReference type="GeneID" id="8291306"/>
    <dbReference type="KEGG" id="lth:KLTH0C04928g"/>
    <dbReference type="HOGENOM" id="CLU_1570902_0_0_1"/>
    <dbReference type="InParanoid" id="C5DDZ0"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="109"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="144"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="170" mass="19228" checksum="5971A23C85A25053" modified="2009-07-28" version="1">MLRYAVLRCAQKHARPRNRCLRASGRGEARSPPRQGRRGHVPIHVRYFVRCLLSMVYVMYMRCRVRLRASAPRRAVGCYNTTGRSDAIWRRRLRNAAVCASAVLAEHQGGSPRSRGPTSSKTPEVAPTVSHGGQKDTRHPYAGVRRRKLRGGFRRRGGSHAFRATKSARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DE92</accession>
    <name>C5DE92_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0C07260p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0C07260g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552541.1">
        <property type="nucleotide sequence ID" value="XM_002552495.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DE92"/>
    <dbReference type="FunCoup" id="C5DE92">
        <property type="interactions" value="358"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DE92"/>
    <dbReference type="GeneID" id="8291410"/>
    <dbReference type="KEGG" id="lth:KLTH0C07260g"/>
    <dbReference type="eggNOG" id="KOG1989">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005611_0_0_1"/>
    <dbReference type="InParanoid" id="C5DE92"/>
    <dbReference type="OMA" id="MIETETH"/>
    <dbReference type="OrthoDB" id="2018507at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000147">
        <property type="term" value="P:actin cortical patch assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14037">
        <property type="entry name" value="STKc_NAK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22967">
        <property type="entry name" value="SERINE/THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22967:SF65">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE AKL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="42"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="391"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="447"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="576"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="621"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="742"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="458"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="481"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="578"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="595"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="624"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="652"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="663"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="747"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="778"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="821"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="870"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="890"/>
            <end position="901"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR22103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="901" mass="101952" checksum="B391B0D65F13E05A" modified="2009-07-28" version="1">MSKSEASSHEVVSGANSSRSATLPVLERLKTGSQVIVGNHRVEIVKYLAEGGFAHIYVVKFVEYANELEQAPSITLKVGETACLKRVLVTDENGLNEMRNEVSVMKQLSGCPNIVQYYDSHASRSRDGVPGFEVMLLMELCPNNSLLDYMNQRLTTKLSEQEILKIMFDVTRALAQMHYLPIPLIHRDVKIENVLVDGDNNFKLCDFGSTSTCLPVANTHQEIAVLTNNIYIHTTPQYRSPEMIDLYRCLPIDEKSDIWALGIFLYKLLFYTTPFELTGQFAILHSKYEFPINNYSSKLINLIIIMLAENPNLRPNIYQIMDNLCSIMKVENPIEDKYGMGPYSFEKYSAYQAKLQKVQYQMYLAYENKQQVDILNDLFINCFEVAPKQPVNVGQKGSHRESQVAAGPQVAELSETGNEGTPAGEEDIGLVQRQFPSVEDLDQYLHTETKRESGRQNSSNIGSLEGSSHSSSKTDLAGAQGSISKLAGNSTNPAQPAVPNPGALSMARESTARSSNSVKQHKPTNPFPYIQNEREVYSLDQDSNSVFHDDGLNTNTEEQFFSQEPVSASAADLKLGQDQAQRSSANVQQPMPTHHFQEQIRDPNVEKIGAFSQYFSATPAPAVHSTTNEPNQTEVPQYYAYQGSQSKNTQPQPQPQPQPQPQPQEQQQQQIKQQQQQQQIQQQLQQQRLQQQQQQQQQQQQQQRQKLQQQQLQQQQLQNQQLQQQQLQQQQLKENEKELLVDFSPAAKKEPQKLDLTYDKVDLSTPRKGGFKEDTNISQQSLASSESYSVELSKSKSESKNRGKTINEVVPQQQQQPRGRRSLDLKYQEMDFSKSPSPDLKLQKVKTITHQQRKSNLGLEAKKSFSRSRKSLDLDHARREAANSSENTSSKRKSFFGKFKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEE8</accession>
    <name>C5DEE8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0C08602p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0C08602g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552597.1">
        <property type="nucleotide sequence ID" value="XM_002552551.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEE8"/>
    <dbReference type="FunCoup" id="C5DEE8">
        <property type="interactions" value="113"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEE8"/>
    <dbReference type="GeneID" id="8291471"/>
    <dbReference type="KEGG" id="lth:KLTH0C08602g"/>
    <dbReference type="eggNOG" id="ENOG502R6DJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_544174_0_0_1"/>
    <dbReference type="InParanoid" id="C5DEE8"/>
    <dbReference type="OMA" id="PGGHYKN"/>
    <dbReference type="OrthoDB" id="4067583at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="13"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="216"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="272"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="324"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="393"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="441"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="180"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="324"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="354"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <begin position="450"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="462"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="551" mass="60742" checksum="DE21E8BD4EA18E88" modified="2009-07-28" version="1">MNTNTKRLSAMIDSFHDDGNDDALYVQRSTPSSPTKSQISKTDTLSRPPALHPAFSSPMSYVNSSPGNRESLISDYSASIHEGVPVSYVVTKAGVPSGKDASVVYSAVNVPVSEETVVREEMLEQDGAESSPVGLALNLKHVSGVRSPLRLASNRSHSKNHSSIGSGNLASESGHSHNFSTISASGSSSAPRFYAQNLESGEGHEENPIHIDQIEEESDAENSKGTSPLEAFHSPVRSLTNRSAVGVAKDSGDVHSMTSSIVPSLHTKVLDSCAQNKDPDRSSTTEYNPSIPPRSRNRPKSHMFIKDGLEDIQNQLQQQILQETENVSRASTNKSSTYYSAADQDNQYNTEEDNFGKRDDDSYYHRPLPTVPVTEKIHRKQASIDREDTIVLPSSERVDPEKPPRLPSLPASVMKASQTSNYDSDDDYEDIVDETFQKAKPNHDSKHVKNTTSSASHQLRTAPHKNKQKAKRKREMRSFDIDTISQMLNVTKGTLIGSEFSNLGMKTEEKRALERLVDSLSRLTADMVLDPERFQEGMRRLEKATRALEGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEJ2</accession>
    <name>C5DEJ2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0C09724p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0C09724g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002552595.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEJ2"/>
    <dbReference type="GeneID" id="8291521"/>
    <dbReference type="KEGG" id="lth:KLTH0C09724g"/>
    <dbReference type="HOGENOM" id="CLU_2850118_0_0_1"/>
    <dbReference type="InParanoid" id="C5DEJ2"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="65" mass="7120" checksum="B576079BBBD49C21" modified="2009-07-28" version="1">MVDEKKPSTIRRLVAVVVSIGVINILIDGCKFILKRALSPVTNAVYGNPDPYSALFKSNNSLFLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEM7</accession>
    <name>C5DEM7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0C10516p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0C10516g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552676.1">
        <property type="nucleotide sequence ID" value="XM_002552630.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEM7"/>
    <dbReference type="FunCoup" id="C5DEM7">
        <property type="interactions" value="234"/>
    </dbReference>
    <dbReference type="GeneID" id="8291557"/>
    <dbReference type="KEGG" id="lth:KLTH0C10516g"/>
    <dbReference type="eggNOG" id="ENOG502QWPB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019691_0_0_1"/>
    <dbReference type="InParanoid" id="C5DEM7"/>
    <dbReference type="OMA" id="CSCTMMH"/>
    <dbReference type="OrthoDB" id="2428527at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009410">
        <property type="term" value="P:response to xenobiotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052478">
        <property type="entry name" value="Metabolite_Synth_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31779">
        <property type="entry name" value="2-NITROPROPANE DIOXYGENASE FAMILY, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G17430)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31779:SF3">
        <property type="entry name" value="PROTEIN RDR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="7">
        <location>
            <begin position="19"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR22238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="561" mass="62413" checksum="ECDCB5AA15B895B9" modified="2009-07-28" version="1">MSTGIPSVPHKKRQRARKACVPCRQRKRRCDGANPCGMCVSYAYKCHYDEQGDPATLPAPLIPEIPDSTRSISPPRTRMVAKPPSIRTKILLNSTQCAADQGVFDAAKSRYMGLSSAVAFARSLGIELQSANPPHLHSFAWNCGLRSEEKSNTHCALHDLITKEETFHFTQIYFSAIHPVFDVVDPEHFKKSVESYWNDGCKVSAFGAVVGGIIALGSLFSGRPGHPRELEIVQYAKNVLEDPTFSRLPSAEQVSAWVLRTLYLRATTRPHAAWLASCVTIHLAEASALHHEVENVELTANDKALPPRTKKITERARRLFWCAWSINSILSYDYGRSSASLGGISCKLPTKSDGNYTAQLVDLALIIPPANTEASKETRVADLLQALTLVYETPDEHNFLSLVKADLCNSFYRRLRLFNYTLDSEVVFQLTTVCNHALSAAYDLVERKLPWWNVLSAPFQYICVLLAINTSESLANVAKAKNIFDRIVSNLNTHMAIEAQNTIKLLLQDSIKKKRQELTDLEAADQAGTASEAPFTAEIDWDVLLDPSHALHLMQQDFSSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEN7</accession>
    <name>C5DEN7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sorting nexin MVP1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Sorting nexin mvp1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0C10758g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the sorting nexin family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552686.1">
        <property type="nucleotide sequence ID" value="XM_002552640.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEN7"/>
    <dbReference type="FunCoup" id="C5DEN7">
        <property type="interactions" value="178"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEN7"/>
    <dbReference type="GeneID" id="8291568"/>
    <dbReference type="KEGG" id="lth:KLTH0C10758g"/>
    <dbReference type="eggNOG" id="KOG2273">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009058_2_0_1"/>
    <dbReference type="InParanoid" id="C5DEN7"/>
    <dbReference type="OMA" id="WEYAGAK"/>
    <dbReference type="OrthoDB" id="10064318at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07597">
        <property type="entry name" value="BAR_SNX8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06866">
        <property type="entry name" value="PX_SNX8_Mvp1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000042">
        <property type="entry name" value="Sorting nexin mvp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028662">
        <property type="entry name" value="SNX8/Mvp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035704">
        <property type="entry name" value="SNX8/Mvp1_PX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045734">
        <property type="entry name" value="Snx8_BAR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47554">
        <property type="entry name" value="SORTING NEXIN MVP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47554:SF1">
        <property type="entry name" value="SORTING NEXIN MVP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19566">
        <property type="entry name" value="Snx8_BAR_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PX" evidence="11">
        <location>
            <begin position="141"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="14"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00072009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR22248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="522" mass="59798" checksum="03F9D5A85F78FE98" modified="2009-07-28" version="1">MSLYDDLEDPWRSEQPTSTSHSANEWTGLNVDPLAGSDVLPSGSLISGINTMTLDSGLGNSGSLPVGSSTLLSAHPENSMEGAWSDSLDEANHRVSNDLNRGEMVAGVPFQSENPHSTEESGKEDYEVWSDGIRKAYNPLAPDIITVEEIPEREGLLFKHTNYLVKHLVELPDTEPSSDRSVIRRYSDFVWLQEVLLKKYPFRMIPDLPPKRIGSHSSDPIFLIKRRNGLSRFINIVVKHPVFKKDDLVLTFLTVPTDLLGWRKQASYDTSDEFTDRKIEASFIKMWRKDMAERWNQADASIDGALELWAKITVLVDRCEKRLRFIAQDRSMLASMLDSFSDSAPQIFASDDGTLLDIKNHLGIVSKHLTTCNNLVKEENMQASSELSPRFRTFTDVLLSLKGLFERYKIMAGNNVPQLQRRIEVNTDRLEQMKDKPDLKGAEYDRVKQSIQRDRKSIAEQMNRSWLVRKCILEEYVYFQETQFLVSDVFQRWVKLNMTFNELNTNEWEKLLDHVQAMPSTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DF67</accession>
    <name>C5DF67_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0D12628p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0D12628g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553260.1">
        <property type="nucleotide sequence ID" value="XM_002553214.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DF67"/>
    <dbReference type="FunCoup" id="C5DF67">
        <property type="interactions" value="80"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DF67"/>
    <dbReference type="GeneID" id="8295500"/>
    <dbReference type="KEGG" id="lth:KLTH0D12628g"/>
    <dbReference type="eggNOG" id="KOG1931">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009596_0_0_1"/>
    <dbReference type="InParanoid" id="C5DF67"/>
    <dbReference type="OMA" id="DYLNAYE"/>
    <dbReference type="OrthoDB" id="10256906at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990071">
        <property type="term" value="C:TRAPPII protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034498">
        <property type="term" value="P:early endosome to Golgi transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056915">
        <property type="entry name" value="Ig_TR130_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056916">
        <property type="entry name" value="NTS_TR130"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045126">
        <property type="entry name" value="TRAPPC10/Trs130"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022233">
        <property type="entry name" value="TRAPPC10/Trs130_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056913">
        <property type="entry name" value="TRAPPC10/Trs130_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13251">
        <property type="entry name" value="EPILEPSY HOLOPROSENCEPHALY CANDIDATE 1/TMEM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13251:SF3">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24966">
        <property type="entry name" value="Ig_TR130_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24967">
        <property type="entry name" value="NTS_TR130"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12584">
        <property type="entry name" value="TRAPPC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23036">
        <property type="entry name" value="TRAPPC10_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="TRAPPC10/Trs130 N-terminal" evidence="5">
        <location>
            <begin position="11"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="Trs130 NTS" evidence="7">
        <location>
            <begin position="306"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="domain" description="Trs130 second Ig-like" evidence="6">
        <location>
            <begin position="676"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="domain" description="TRAPPC10/Trs130 C-terminal" evidence="4">
        <location>
            <begin position="999"/>
            <end position="1083"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF24966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF24967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR22822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1101" mass="126473" checksum="FE6BF886748F8C70" modified="2009-07-28" version="1">MSDKHLTNPVCFSYFDPFNAFGSIQSELEDRLPFRNLHWKPSNQNLRTIAHLPIEIIPETDESMKKCGSEPLIMFLVIICTSIDDYRAKVRPLIRQWLPPPGSNKDAGSNVSEAPAKRIVLLHSNSDISETNLFKTVSFYDKFSKDFPFLTAIEVKSIYKSEKEKTNFWNSTVNQLRKYTMEVFQQRLGYLETKLRKVPEGNTMELASLQESILNLFLAFHLNDEASKELESLRHTLFTQLGPKLSKGELEVPFQFSHMEPDVSKNSIAFQLAKENLTIYQLNRFFLIKQCELIQKSYQQTARNLRLYQLVRSFLWVIQNEFNESPMIAQFKYSFLESLNHAGIFDIEKTTTFREIQADFEIIQRDCWLDMAFGQHSFRLNGRIYPPNKVSCSSDDLKSSFENEDVFQLSFLERTKNIITLLNECNSKRYRIVDLYSVEVALLYHQRKEYQKAIDILQSCHEYYKDSCWNELAIKLLECFVDCLIKCPETHVITLGEEDVPVATVLSNSILDLLASTQSGERKAFWWDLFLSINREGGDSLMYPLDNLFEIKVENELHIARPNVYALRVKVSSQKLPQDVSVAIMRVLLKNDLDEFLEFKLIDTVIRPGDNEVYLEATEISFGNFEIISTESTVGNTIFCKEFSGPCASISLVKPLSSHNFGVAVLSSKRLELTKNSIHLKYSNVDIPEKFKLVLTIIPPQGEAQSPVAFSADGKKLSVTVTGFDTSYVEYFILHPIDEFTLKQELSFNVIASPNHNFYEKKVEKVSCALPLSISVEDIARENCFFFKFLISSSLPTEPVLFYKSCLESYEPKKYTVTGGFEPEAPILLRNRLNETSLSFFKIATQDGAKFDSVDSFQLKVRFSTLKGQIDHLVTSAILIQGYPDIASKMELYRDVWNSSVLGALSYDYDLFESDNIIKLVSSKESVEAIRKLLVTRVRDEGFLKASSRCLFELYKGFQLSHIEIKEYTKDLETSELVVGVHLPSPSKFFSVNLKAETTCEQNLKVGQLLPVDIQIDDLSSCWASESRQEKNYIFELSNSSEWIINGKRRFCLCPGKSEYRVHMIPLRRGYLKYPRVEISEDGKKAPEVYYSNMHETILIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFD1</accession>
    <name>C5DFD1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Elongator complex protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0D14146g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the elongator complex which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine). The elongator complex catalyzes formation of carboxymethyluridine in the wobble base at position 34 in tRNAs.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the ELP1/IKA1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553324.1">
        <property type="nucleotide sequence ID" value="XM_002553278.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DFD1">
        <property type="interactions" value="1173"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFD1"/>
    <dbReference type="GeneID" id="8295568"/>
    <dbReference type="KEGG" id="lth:KLTH0D14146g"/>
    <dbReference type="eggNOG" id="KOG1920">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001477_0_1_1"/>
    <dbReference type="InParanoid" id="C5DFD1"/>
    <dbReference type="OMA" id="WRESLYC"/>
    <dbReference type="OrthoDB" id="40048at2759"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033588">
        <property type="term" value="C:elongator holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002926">
        <property type="term" value="P:tRNA wobble base 5-methoxycarbonylmethyl-2-thiouridinylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056167">
        <property type="entry name" value="A-sol_ELP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056164">
        <property type="entry name" value="Beta-prop_ELP1_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056165">
        <property type="entry name" value="Beta-prop_ELP1_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006849">
        <property type="entry name" value="Elp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056169">
        <property type="entry name" value="HB_ELP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056166">
        <property type="entry name" value="TPR_ELP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12747">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12747:SF0">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23925">
        <property type="entry name" value="A-sol_ELP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04762">
        <property type="entry name" value="Beta-prop_ELP1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23797">
        <property type="entry name" value="Beta-prop_ELP1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23936">
        <property type="entry name" value="HB_ELP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23878">
        <property type="entry name" value="TPR_ELP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017233">
        <property type="entry name" value="IKAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="ELP1 first N-terminal beta-propeller" evidence="8">
        <location>
            <begin position="1"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="ELP1 N-terminal second beta-propeller" evidence="9">
        <location>
            <begin position="419"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="domain" description="ELP1 alpha-solenoid" evidence="11">
        <location>
            <begin position="717"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="domain" description="ELP1 TPR" evidence="10">
        <location>
            <begin position="940"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="domain" description="ELP1 three-helical bundle" evidence="12">
        <location>
            <begin position="1112"/>
            <end position="1284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1188"/>
            <end position="1219"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="943"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1188"/>
            <end position="1202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR017233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF23925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF23936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR22886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1323" mass="150183" checksum="3400244D22B63591" modified="2009-07-28" version="1">MRNLITLNRGRFKPQSFEVPDLRLYGSVFDTLSDSITCVLGDYDLGAFEVHQYMKDGSCNTLASFNLQTLGGELLSFAHFGDLGQLVFVMSQGDIITASYDTVGFDPSFAVVEIVGSIDGGISAAEWSYDEETLALVTAERNVVLLSRQFDPIAEYKLEEDDLKMSKHVNVGWGKKETQFRGKGARAMEREALESLKASGLVGDELRDPTMPYLVDKGTVSAMDSRKASISWRGDCEYFVVSTIEAVHEESDEPFERRVLRVFSRDGQLQNVSEPVDGLEDLVSWKPQGSLIASVQRRDQIPGEESVDLVFFERNGLRHGEFDTRLSLNEKIQALCWNCNSEVLAIVLADRIQLWTTKNYHWYLKQEVYANDIKFVKWHPEKDFTLMFGDEDYVNVVDFAYKLALGPTLEPSDSGTVLVVDGCTVNITPLGLANVPPPAYYRDFDAPGNVLDAATSLSNELYVAITNNELVLATVPSLEDMKKGLQPCVVSRLHKSILATEYDSLRQVAFMNDNVLGVLLDADNLSRIALIDVTDITQPTVFNIIETFDKVVLLRTSFEYNYMVYETKDSRVVQLDQEGQTVEVAKFPQLVRDLRVKRVENSKAVDEDDRLAEDSKIVAFGLAANGKLYANDILLTSTVTSMEVTEKFLMFTTAQHNLQFVHLNSTEFKALPIVEDSVADERVRAIEMGSTLVTAMPSRSSVVLQAARGNLETIAPRIMVLAGVRKSILAKKYLEALMSCRTHRVHLDILHDYAPDLFYENLEHFINDIGKGEHLDLFISCLTEEDVTITKYKETLRASMDERFELAPSAPTEMELYMKKKMFNPELSKVNKICNALLEVFLNNPDYKKKYIQSILTAYACQNPPNLEAAMMLISSLEEESDTCLTYLCFLHDVNQLYKVALSLYDIKLTLNIAQKSQMDPREYLPFLQELHEKEPLVRQFMIDDYLKRYEKALDHLSALEKDSKRLSQKFIDYVQTHDLYIYALSMFRYEPDKQNDIYHIYAPFLSSKQEYGEAAVIYEMLGENREAVEAYTLGKKWCEALALAALKFSDDVPSVADQLVSSLSFDHRFEEAAQIEFVYLKNTREAVKLYCKAYSYEKATLVAVNDKHPELIEEVLDPGLGEGFGTIAELLADCQSQVNSQLRRLRELRQKKGDDPYAFYGQETEQADDVSIAASETSTKESFFTRYTGKTGGTAKTGASRRTAKNKRREERKRARGKKGTIYEEEYLIQSIGRLIDRLTQTEPDALKLIEALLRRRRREEAYQIQKRFVEVTSLLKDNVAEIYTLSEKDRERVDEDGNIYYVPEIPVPTIKDFPALKTVGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFE2</accession>
    <name>C5DFE2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0D14388p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0D14388g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553335.1">
        <property type="nucleotide sequence ID" value="XM_002553289.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFE2"/>
    <dbReference type="GeneID" id="8295579"/>
    <dbReference type="KEGG" id="lth:KLTH0D14388g"/>
    <dbReference type="eggNOG" id="ENOG502S1X2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078308_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFE2"/>
    <dbReference type="OMA" id="ICPNDNA"/>
    <dbReference type="OrthoDB" id="4095829at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008427">
        <property type="entry name" value="Extracellular_membr_CFEM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05730">
        <property type="entry name" value="CFEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00747">
        <property type="entry name" value="CFEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52012">
        <property type="entry name" value="CFEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002947817" evidence="7">
        <location>
            <begin position="23"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="CFEM" evidence="8">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="93"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="42"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS52012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR22897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="260" mass="24072" checksum="AF35DBED1606A139" modified="2009-07-28" version="1">MRSTIALSAAISVVGFASGVLATPPACLLACVAQVTKKSSTCDLDAVSCVCEKQAGDVKSCLDSACPNGDADSAYSAFKSSCSEQNASVSSASSSASKSSSASTSHSSSSSSASSTSSASKSSSASSTSASASSSSSSASPSSSSASPSSSSASSSNSSAPASSSSASASGTSASASSSSSSASQSSSAPASSSSAPVSSSSAVSSTAISSSTTPSFSSYSSATSSSSSEVVISTQTDAAGALGVGSGLVGGLLVAGMLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFF5</accession>
    <name>C5DFF5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0D14674p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0D14674g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553348.1">
        <property type="nucleotide sequence ID" value="XM_002553302.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFF5"/>
    <dbReference type="STRING" id="559295.C5DFF5"/>
    <dbReference type="GeneID" id="8295592"/>
    <dbReference type="KEGG" id="lth:KLTH0D14674g"/>
    <dbReference type="eggNOG" id="KOG1962">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_742001_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFF5"/>
    <dbReference type="OrthoDB" id="435607at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070973">
        <property type="term" value="P:protein localization to endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008417">
        <property type="entry name" value="BAP29/BAP31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040463">
        <property type="entry name" value="BAP29/BAP31_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12701">
        <property type="entry name" value="BCR-ASSOCIATED PROTEIN, BAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12701:SF20">
        <property type="entry name" value="ENDOPLASMIC RETICULUM TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05529">
        <property type="entry name" value="Bap31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="202"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="243"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="301"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="BAP29/BAP31 transmembrane" evidence="7">
        <location>
            <begin position="200"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="340"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR22910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="373" mass="42655" checksum="FE7915CF093FD3BF" modified="2009-07-28" version="1">MSGSTDVVLLQRQQTWRGVTRLTRPPARESAFCADVYAFCHVQQNTHVTRCRGMTSGRRGRVGEKVEKAREKRAWLNFLSQWSVRPRRAPRGGYSCLSGLCWHRSSPRKLACFRLGGNVHRKSNEENARHACVITNEENVMAYVKVLPGFKFNQSLKTETAISTRTLFPVLRRPFKTSSIPLLTTRKRLNACPVPSRTPMTLYYTFVFGILAFEMVMFLLLAMPVPSKFRKPITMALIKPFRLTQVQVAIKCVLAFILLLFIDTINRVYSINSELSQTPLASGVSDRNEVQSRKFYAQRNMYLTGITLFLTFTVFRTYGLVWELLEMKEKFRAVPNQMNVEDLQKQIEAADKEIASMKERAEGLQADMSGSNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFH5</accession>
    <name>C5DFH5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0D15136p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0D15136g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553368.1">
        <property type="nucleotide sequence ID" value="XM_002553322.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFH5"/>
    <dbReference type="FunCoup" id="C5DFH5">
        <property type="interactions" value="310"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFH5"/>
    <dbReference type="GeneID" id="8295612"/>
    <dbReference type="KEGG" id="lth:KLTH0D15136g"/>
    <dbReference type="eggNOG" id="ENOG502QX5C">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041192_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFH5"/>
    <dbReference type="OMA" id="SDSPCWR"/>
    <dbReference type="OrthoDB" id="2162994at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="3">
        <location>
            <begin position="432"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="242"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="280"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="280"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="326"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="363"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="409"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="421"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR22930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="528" mass="57797" checksum="B2E87698FC09E7D8" modified="2009-07-28" version="1">MSTFSAPMVPTVSAGPETNTRRKRSFDDLLLPSLGFDSSHVYNHESAYLFQPVALDRHNKRARTAPASPTGVGSLTPHTSPLLNKGTINPISLPPVPSVLNLAQSGAPTTLRESLSNPEYIPSTPTLSRVAHRDSFIKPSQKGPLPSLRHLRLLPHPKVQEHAYRFPDTSENTPIWRESLMNWCKSENYQDYQQIAKEVQRCVYSDSAPGLNILANVATITQKIPSILNPVDQFHDLSDSASTDGVVTPPMSPRNATAAGRSGPLTFTPTLSEKLVQTIRQKRAGSHRKTNSFKAREMKKLLNNRDVLSIDSKGKVEKPARKRKTSSPSSPQQLVMKISTLSPLRTPLSGTALPKGPAMGATPPTSRNSITRTPQRSVSPVRAVSPPRTHVFVLNEPRTPPMSSHTVASSGRSGGSPRSEACQTKKTTSPKRTSNRTCISCLSSDSPCWRPSWTNKKQDQLCNSCGLRYKKTQTRCLNETCRKIPSKGELAIMKANGKITRTNSDGTLTEGLGCLFCNSMVEIREKPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2023-06-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFP0</accession>
    <name>C5DFP0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0D16676p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0D16676g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553433.1">
        <property type="nucleotide sequence ID" value="XM_002553387.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFP0"/>
    <dbReference type="GeneID" id="8295681"/>
    <dbReference type="KEGG" id="lth:KLTH0D16676g"/>
    <dbReference type="HOGENOM" id="CLU_2171530_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFP0"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR22995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="110" mass="11674" checksum="6C37B239CC22AC79" modified="2009-07-28" version="1">MTEKYFECLESFTNDPSEGTVVSRSARGRALAAQSHRKLDQSSCLAPSIVLGSGRSSCLALGGRLLALPTQPLPPGGHSLSVGRDACTTMLQYSVGKKMEVDDTGLYRIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFT4</accession>
    <name>C5DFT4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0D17754p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0D17754g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR23039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553477.1">
        <property type="nucleotide sequence ID" value="XM_002553431.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFT4"/>
    <dbReference type="FunCoup" id="C5DFT4">
        <property type="interactions" value="1431"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFT4"/>
    <dbReference type="GeneID" id="8295728"/>
    <dbReference type="KEGG" id="lth:KLTH0D17754g"/>
    <dbReference type="eggNOG" id="KOG1991">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004196_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFT4"/>
    <dbReference type="OMA" id="WVAKTSW"/>
    <dbReference type="OrthoDB" id="760868at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000244">
        <property type="entry name" value="Nonsense-mediated mRNA decay protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997:SF18">
        <property type="entry name" value="D-IMPORTIN 7_RANBP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997">
        <property type="entry name" value="IMPORTIN-7, 8, 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="7">
        <location>
            <begin position="24"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR23039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1044" mass="118930" checksum="3263423026D1A441" modified="2009-07-28" version="1">MDPNTLLQCFAGTLNHDHSIRTEAESQLKIAGGTPGFLGACLDIISAADIPDNIKLSASLYFKNKILYGWSGKKHGKNELLDFTVDNDEKPVVKDMLVKALVQSSIYSPNCIRLLQPALSTIVGEDYPQKRWDSLLDSSFGLMDSNDINSAHIGLLCLSEIFRTYRWKDNDSRQELEHIIVKFFPPLLEFATSNLLSEGRNVENAKVGDMVKLVLKIYKFVTYHDLPFTLQRPENFISWANFHVAIIQQSLPTQILASMDTESRKSFSWIKSKKWAYANMSRLFQRYASTSLSRKFAYDEFKTLYLQEFLPQLLQLFFQQIEQWSSKSLWLSDEAIYHILGYIEQTVIQKATWPLVKPHFTIILEHVIYPLLCPTEKTLETFESDPQEYIHRNLELWDDNYSPDFAALSLLSTCVTKRGKSTLVPTVQFVTEKLKQDLNDFNNSEKVIKAESSLRIISNILDRLFQKNSAFANEAEDILSSFVFPFFLSSQEFLKARVCEICSKLGDYQFRNEHILQTIYNGIISCFMQDSDCLPVELLAALALQAFIHVPQFQEPISANVVQMTQKLLRISNEIESDAISGVLQEFVECFSEQLQPFGIELMNDLVQQFLKLAIDLQDSSNFDINLITDKSDLPDDTDKQMAALGILSTAISILLSFENSHEIVKSLEQSFYPAAEFILKNQMEDFYGEVCEFLENSTFLLRDISPISWKVLELIGECNRKDESMVSFYLDDFMVAFNNYLVYGQDELKKNQFYSNILFEVYTKATVSDDNGLDEMVSIFELSQKLVLALGPQTPNEYIERFLGDALASIKSEAQHIKENISFSVSAFNVICACLTCYPLNTINILNASGALESFFSMWFEFFVPNYKRVYDIKLSAMALLALVNEVELQDLVSLSVANVLPKSSSMIVKLLTTFPHALKALEEKRKEYSSDTLQEGANIDWEDAGQFDDASDEDGENDEEYLEFLNKDLKFVEEETNGYEPAESFEDLEEDPLSGSVIDSINVYQVLKSVISALQGDQVKYQAFTSGLSPEDQQTFLQLVNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFW2</accession>
    <name>C5DFW2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">pyruvate decarboxylase</fullName>
            <ecNumber evidence="5">4.1.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">KLTH0D00418g</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2-oxocarboxylate + H(+) = an aldehyde + CO2</text>
            <dbReference type="Rhea" id="RHEA:11628"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="EC" id="4.1.1.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>pyruvate + H(+) = acetaldehyde + CO2</text>
            <dbReference type="Rhea" id="RHEA:45484"/>
            <dbReference type="ChEBI" id="CHEBI:15343"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="12">Binds 1 Mg(2+) per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>a metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:25213"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the TPP enzyme family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.1" evidence="5"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552742.1">
        <property type="nucleotide sequence ID" value="XM_002552696.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFW2"/>
    <dbReference type="STRING" id="559295.C5DFW2"/>
    <dbReference type="GeneID" id="8294961"/>
    <dbReference type="KEGG" id="lth:KLTH0D00418g"/>
    <dbReference type="eggNOG" id="KOG1184">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013748_0_2_1"/>
    <dbReference type="InParanoid" id="C5DFW2"/>
    <dbReference type="OMA" id="IFWGQVS"/>
    <dbReference type="OrthoDB" id="4049618at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004737">
        <property type="term" value="F:pyruvate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000949">
        <property type="term" value="P:aromatic amino acid family catabolic process to alcohol via Ehrlich pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02005">
        <property type="entry name" value="TPP_PDC_IPDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07038">
        <property type="entry name" value="TPP_PYR_PDC_IPDC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1220:FF:000009">
        <property type="entry name" value="Pyruvate decarboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000019">
        <property type="entry name" value="Pyruvate decarboxylase isozyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000024">
        <property type="entry name" value="Pyruvate decarboxylase isozyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012110">
        <property type="entry name" value="PDC/IPDC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000399">
        <property type="entry name" value="TPP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047214">
        <property type="entry name" value="TPP_PDC_IPDC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047213">
        <property type="entry name" value="TPP_PYR_PDC_IPDC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43452">
        <property type="entry name" value="PYRUVATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43452:SF1">
        <property type="entry name" value="PYRUVATE DECARBOXYLASE C186.09-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036565">
        <property type="entry name" value="Pyruvt_ip_decrb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00187">
        <property type="entry name" value="TPP_ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="10" id="KW-0456">Lyase</keyword>
    <keyword evidence="8 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 13" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme N-terminal TPP-binding" evidence="16">
        <location>
            <begin position="3"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme central" evidence="14">
        <location>
            <begin position="199"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="15">
        <location>
            <begin position="407"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="438"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="465"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="467"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR036565-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAR22304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="561" mass="61317" checksum="8A31604C6D620C59" modified="2009-07-28" version="1">MTTVGNYLATRLVQAGIKNHFTVPGDYNLVLLDKLQEHPELEEVNCCNELNCSFAAEGYARTKGIAAVVVTFSVGAFSAFNGIGSAYGENLPVILISGSPNTNDSSDHRLLHHTIGTHNYDYQYEMAKQITCAAVQIKYPEDAPSLIDYAIRECLAKKKPCYIEVPTNMASAICAPPGPISSLVNSVPTPKDVLSQAVNASVDFINSHLKPTLLAGPKLKCADAQKAFLRLAEALKCAVAVQPAAKSMFPEDHPQFAGVYWGQVSTKKADAILEWSDLTICAGCIFTDYSTTGWTALQPSSHRLEADADNVRFPGHSFDQIGLANFLNVLAERVSENDKSLIEYNRLRQSPPLIKAADPKAAITRKEMARQIQQLITPKTTLFAETGDSWFNGVQMDLPKGALFEVEMQWGHIGWSVPASLGYAVGAPDRRIITMVGDGSFQMTAQEVSLMVRLRKPIIIFLINNYGYTIEVEIHDGPYNNIKNWNYAALMEVFNANEGSGKGFKARTGGELADAIEKAQANVSGPTLIEIQINRDDCTNELITWGHFVALANARPPKKTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DG87</accession>
    <name>C5DG87_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Translation initiation factor eIF2B subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">eIF2B GDP-GTP exchange factor subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0D03234g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="8">Component of the translation initiation factor 2B (eIF2B) complex which is a heterodecamer of two sets of five different subunits: alpha, beta, gamma, delta and epsilon. Subunits alpha, beta and delta comprise a regulatory subcomplex and subunits epsilon and gamma comprise a catalytic subcomplex. Within the complex, the hexameric regulatory complex resides at the center, with the two heterodimeric catalytic subcomplexes bound on opposite sides.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the eIF-2B alpha/beta/delta subunits family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552867.1">
        <property type="nucleotide sequence ID" value="XM_002552821.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DG87"/>
    <dbReference type="FunCoup" id="C5DG87">
        <property type="interactions" value="1248"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DG87"/>
    <dbReference type="GeneID" id="8295087"/>
    <dbReference type="KEGG" id="lth:KLTH0D03234g"/>
    <dbReference type="eggNOG" id="KOG1466">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016218_0_2_1"/>
    <dbReference type="InParanoid" id="C5DG87"/>
    <dbReference type="OMA" id="GDWESCK"/>
    <dbReference type="OrthoDB" id="10249309at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005851">
        <property type="term" value="C:eukaryotic translation initiation factor 2B complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10470:FF:000017">
        <property type="entry name" value="eIF2B 34 kDa alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1070:FF:000002">
        <property type="entry name" value="Translation initiation factor eIF-2B subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10470">
        <property type="entry name" value="Translation initiation factor eif-2b, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1070">
        <property type="entry name" value="Translation initiation factor eIF-2B, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051501">
        <property type="entry name" value="eIF2B_alpha/beta/delta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042528">
        <property type="entry name" value="elF-2B_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000649">
        <property type="entry name" value="IF-2B-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042529">
        <property type="entry name" value="IF_2B-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45860">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45860:SF1">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01008">
        <property type="entry name" value="IF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR22429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="305" mass="33997" checksum="2277A8395E0E0BA7" modified="2009-07-28" version="1">MSEEFNITETYLKFLEEDSEMTMPIAAIESLVTMLRVKNPETSAEMINTIKSAADTLTSSIPNSMSLRAGCDIFMRFVFRNTHLYGDWESCKKHLIENGQLFVSRANRSREKIAQIGLDFISDDDIILVHGFSRAVYSVLSHAASKFIRFRCVVTESRPTKQGAQLYKLLQEKNIPVKMVVDSAVGSIIHKVDKVLVGAEGVAESGGVINLVGTYSIGVLAKNTNKPFYVVSESHKFVRMFPLSPDDLPATAGPLDFTRHEDCEQSLLEPAIDYTSHEYITALITDLGVLTPSAVSEELIKMWYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGE9</accession>
    <name>C5DGE9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ATP-dependent DNA helicase II subunit 2</fullName>
            <ecNumber evidence="4">3.6.4.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">ATP-dependent DNA helicase II subunit Ku80</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ordered locus">KLTH0D04774g</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ku80 family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="4"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552929.1">
        <property type="nucleotide sequence ID" value="XM_002552883.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGE9"/>
    <dbReference type="FunCoup" id="C5DGE9">
        <property type="interactions" value="179"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGE9"/>
    <dbReference type="GeneID" id="8295156"/>
    <dbReference type="KEGG" id="lth:KLTH0D04774g"/>
    <dbReference type="eggNOG" id="KOG2326">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029650_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGE9"/>
    <dbReference type="OMA" id="DIRGFMD"/>
    <dbReference type="OrthoDB" id="30826at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043564">
        <property type="term" value="C:Ku70:Ku80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042162">
        <property type="term" value="F:telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00873">
        <property type="entry name" value="KU80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006164">
        <property type="entry name" value="DNA_bd_Ku70/Ku80"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024193">
        <property type="entry name" value="Ku80"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005161">
        <property type="entry name" value="Ku_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016194">
        <property type="entry name" value="SPOC-like_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12604">
        <property type="entry name" value="KU AUTOANTIGEN DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12604:SF4">
        <property type="entry name" value="X-RAY REPAIR CROSS-COMPLEMENTING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02735">
        <property type="entry name" value="Ku"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03731">
        <property type="entry name" value="Ku_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00559">
        <property type="entry name" value="Ku78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100939">
        <property type="entry name" value="SPOC domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="14" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0347">Helicase</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0779">Telomere</keyword>
    <feature type="domain" description="Ku" evidence="19">
        <location>
            <begin position="286"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="570"/>
            <end position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00031847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CAR22491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="602" mass="66950" checksum="8877C8D096CAC280" modified="2009-07-28" version="1">MGSEATTFIVDVSPSMVGLGYVEQATAYIEYTLFLKSKKQRKTDWVNCFLANCPYSRNNHETANVFELMPFTAPIGAHKVIEILKELKSLVTRSAPGKHNINSMVQCLLVSSVSMRDEFKKRKVRKRIIVFTDNLDGLDLTEEELATLEQELDCSIVLVTCGLKVEERKTSEIPIWEKCIQKISGSMEVSMTALLQEISTHRPAVVKPVRIFQGELRLGAPISDEDVTGPSYDSITVKVEGYPATKAVSSLNRRVVAKSEDGTFGPVKSVIEYEAIDDGEDSEGGVASVSKEYVTKAYRYGSDYVVLPQAIESERTYKTKPGLDLRGFVGNESIPRHFLSSESTFIIPATKDGTRADFVSFSALVDSMIKLRKFAIARYVQKLNGEVQMCLLCPILIPKKRKEPGDDETYTRALVLNRLPFAEDERCSDYPRLAQAAASSDAETAEIDALMGELIDTMDLGPGPDQTWCSTTLYRSFDPTVLEPTLPLPRPTEPRDSSVQDALCVPAIGIYRQQQILIEYMHQRIVQGSADFEVPGLPNDLQKKLFPSNELSKGTLLKLQELLAIEVNNSRASSATPNDEQQDEDEFQEIPSLESLLARGRT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-05-31" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGS4</accession>
    <name>SPC42_LACTC</name>
    <protein>
        <recommendedName>
            <fullName>Spindle pole body component SPC42</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">SPC42</name>
        <name type="ordered locus">KLTH0D07854g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Forms a polymeric layer at the periphery of the spindle pole body (SPB) central plaque which has an essential function during SPB duplication and may facilitate attachment of the SPB to the nuclear membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Spindle pole body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SPC42 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553054.1">
        <property type="nucleotide sequence ID" value="XM_002553008.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGS4"/>
    <dbReference type="SMR" id="C5DGS4"/>
    <dbReference type="FunCoup" id="C5DGS4">
        <property type="interactions" value="213"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGS4"/>
    <dbReference type="GeneID" id="8295287"/>
    <dbReference type="KEGG" id="lth:KLTH0D07854g"/>
    <dbReference type="eggNOG" id="ENOG502RYX7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056211_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGS4"/>
    <dbReference type="OMA" id="HNHATHR"/>
    <dbReference type="OrthoDB" id="4061426at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005816">
        <property type="term" value="C:spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1180">
        <property type="entry name" value="Geminin coiled-coil domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021611">
        <property type="entry name" value="Spc42"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11544">
        <property type="entry name" value="Spc42p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0175">Coiled coil</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0206">Cytoskeleton</keyword>
    <keyword id="KW-0539">Nucleus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000409210" description="Spindle pole body component SPC42">
        <location>
            <begin position="1"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="135"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="189"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="310"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="82"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="226"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="16"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="203"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="310"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="358" mass="39523" checksum="D73E6BF533CD94D9" modified="2009-07-28" version="1">MSPHTEGPLFAVLKNSRARPRPRESRLRRRVHKQMNISPTPKRYNSRGYEFPRSNDEAFHPFNREPPMVPSERILPEEYRVNSQMINKLIKQNKDLTQQLDRKQDEIDRLNVLVGSLRGKLIKYTELNKKLEQAAAGARQSSPDDVLQVNRTRPREPPAGGTRLDDRLSDLYSKLETLTELVVGSAAADRGARDHVHVPSAGATRTPSSGASVSHRPLQPGAASEDDILTQESAELKGLEDQIDLLKRKLLIKRENELRKLSLNKELLDLMEKLDVSRPAGPVPSSSTPPHCDQCHKAAYQTAAANGGHGVPAGAGSGAGAGASMPSPRNPPYMSMAQVLETPTPAHRSSHKPNDTLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGU8</accession>
    <name>C5DGU8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0D08448p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0D08448g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553078.1">
        <property type="nucleotide sequence ID" value="XM_002553032.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGU8"/>
    <dbReference type="FunCoup" id="C5DGU8">
        <property type="interactions" value="159"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGU8"/>
    <dbReference type="GeneID" id="8295314"/>
    <dbReference type="KEGG" id="lth:KLTH0D08448g"/>
    <dbReference type="eggNOG" id="KOG0725">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_2_10_1"/>
    <dbReference type="InParanoid" id="C5DGU8"/>
    <dbReference type="OMA" id="QETETWG"/>
    <dbReference type="OrthoDB" id="417891at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760:SF133">
        <property type="entry name" value="3-OXOACYL-[ACYL-CARRIER-PROTEIN] REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASES/REDUCTASES FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR22640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="265" mass="28683" checksum="594478BF718B3FA5" modified="2009-07-28" version="1">MPRTPVALITGATRGIGRAIVDKLSAEGLSCLMVGSRKESFGPMTADLPVICRELQWHRGLVVDLAQWPQWTQQGRFDGFHFSSVPGARAYRCSDGAWPLLDLPPSSELALLVNCAGVTQASPALRCDASEIQRITNINYLSAVSLCNMAAKRMLRSRKHLSRPPCIVNISSILGDPATSALPGTALYSASKAALAQFSRTLSAELARAGIRVHCLSPSLVAQTDMVQTLEEDAKVRLESTFSQFPAQSPQQIASQVWKLYDSDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGW5</accession>
    <name>C5DGW5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0D08822p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0D08822g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transcriptional coactivator PC4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553095.1">
        <property type="nucleotide sequence ID" value="XM_002553049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGW5"/>
    <dbReference type="FunCoup" id="C5DGW5">
        <property type="interactions" value="85"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGW5"/>
    <dbReference type="GeneID" id="8295331"/>
    <dbReference type="KEGG" id="lth:KLTH0D08822g"/>
    <dbReference type="eggNOG" id="KOG2712">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065855_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGW5"/>
    <dbReference type="OMA" id="MNRADDD"/>
    <dbReference type="OrthoDB" id="2505440at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060261">
        <property type="term" value="P:positive regulation of transcription initiation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.31.10">
        <property type="entry name" value="Transcriptional Coactivator Pc4, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003173">
        <property type="entry name" value="PC4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009044">
        <property type="entry name" value="ssDNA-bd_transcriptional_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045125">
        <property type="entry name" value="Sub1/Tcp4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13215">
        <property type="entry name" value="RNA POLYMERASE II TRANSCRIPTIONAL COACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02229">
        <property type="entry name" value="PC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54447">
        <property type="entry name" value="ssDNA-binding transcriptional regulator domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcriptional coactivator p15 (PC4) C-terminal" evidence="8">
        <location>
            <begin position="30"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="100"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="152"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="226"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="243"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="256"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR22657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="266" mass="30044" checksum="B048F01B1AACBCC1" modified="2009-07-28" version="1">MSYYNRYRGQRKFAGNGETGAAIDEPDAIFELGNNKRVTVRQFRNINLIDIREYYADSASGEMKPGKKGISLTEELYDELLKHRQNIDDALRRFGSKRPRTKTVNVLSDSEDEPQSMRVAPPQRSKPTRSEQSKPARRDRRTAAPAPPQSQPDEKRRKRDAREAGARLVMPGGQPSNPRLDDLHTSRTNVSNPRLDDLHTPPASASNPRLDDHLPRLPSVPKPDNDSDLDSSDEDFALALESEFQRDENSARANDHEDEGEISEES</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5DH46</accession>
    <name>C5DH46_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0E01276p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0E01276g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553544.1">
        <property type="nucleotide sequence ID" value="XM_002553498.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DH46"/>
    <dbReference type="FunCoup" id="C5DH46">
        <property type="interactions" value="44"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DH46"/>
    <dbReference type="GeneID" id="8291682"/>
    <dbReference type="KEGG" id="lth:KLTH0E01276g"/>
    <dbReference type="eggNOG" id="KOG1703">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016772_0_0_1"/>
    <dbReference type="InParanoid" id="C5DH46"/>
    <dbReference type="OMA" id="IYGSPFP"/>
    <dbReference type="OrthoDB" id="1112565at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030695">
        <property type="term" value="F:GTPase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08368">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09397">
        <property type="entry name" value="LIM1_UF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047120">
        <property type="entry name" value="Pk/Esn/Tes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24211">
        <property type="entry name" value="LIM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24211:SF22">
        <property type="entry name" value="TESTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0440">LIM domain</keyword>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="5">
        <location>
            <begin position="689"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="34"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="112"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="399"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="466"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="644"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="64"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="187"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="203"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="228"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="254"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="399"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="466"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="644"/>
            <end position="661"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR23107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="837" mass="92754" checksum="7AA54D674EA2289E" modified="2009-07-28" version="1">MQGWIYSSPFPALNPKVRYKTALERAGFDVNFHDKNKPREAHGSVLPAGRHVSEGGRDVFGTRSAPNLAQTAQRAVSERDPRGYQNASALRSPPHSPFVKERSAPALQEHYKVRRSDESKGAFSPRVGQPQEGRNFASAGSHKASPQPAAFPQLRKDSQSRGALDSRFQRHGSRTSPDRASLKSARTRGSSISQGRSAGEGSFQVSSAPQDRFSASETARRGPVARQSPASAAGAAGKESPVSQARLSTEDLGNRQSSVFDFENQRNSPAERNFRSPDIDPVERSFKQLTQNDNDSFSSQQEREFTPYARDSFTSVNQSDEVFEDAADIDDFNEKGDNASKWNSIMRLSKAPEDSVQEAAPLDRDSTNEYVDATPRTRPTSTSSISFTPVAELHVNLSPNRPTEQTESINSATDAPISQAEPIGLERESSASVSGVYDIDLASGHTDLSQGVPQFSIQPTYHFSEENVGREQELREEQEVDTEEGTAQDPTATVKSSLQVERLLAQLDNVSLNRNLSVGNRRHSPGSSRFKKSSAYLSGFVSNPAATGIDAKLQTGIASGDDQQFTPQVLSVAEDRAHALSGEISPGSDDSPQFYHIRRQDEPRQADHRESDMTDFMRPSQQLTESLKVPVIQPLQLYQLENTAEARAETAKPETKQEPHRLSSSSSLVEGSQPAEPPVPVFKYPPGEGPCRACGLEVTSRRIYSKKANELSGQWHRPCFKCIKCELTFSKKVVCYILDDEPYCQRHFHETNNSICRICQGFIEGKCLENDRDERFHVDCLKCFRCGEFICEDYFLFNNELPLCANHDIEALKLESFGNMGQNNTVSKRRTRIINFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>C5DH90</accession>
    <name>C5DH90_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0E02310p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0E02310g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553588.1">
        <property type="nucleotide sequence ID" value="XM_002553542.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DH90"/>
    <dbReference type="FunCoup" id="C5DH90">
        <property type="interactions" value="25"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DH90"/>
    <dbReference type="GeneID" id="8291729"/>
    <dbReference type="KEGG" id="lth:KLTH0E02310g"/>
    <dbReference type="eggNOG" id="ENOG502RJKI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031414_1_0_1"/>
    <dbReference type="InParanoid" id="C5DH90"/>
    <dbReference type="OMA" id="WNDYHET"/>
    <dbReference type="OrthoDB" id="4033945at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071467">
        <property type="term" value="P:cellular response to pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014844">
        <property type="entry name" value="PalH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35779">
        <property type="entry name" value="PH-RESPONSE REGULATOR PROTEIN PALH/RIM21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35779:SF2">
        <property type="entry name" value="PROTEIN DFG16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08733">
        <property type="entry name" value="PalH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="156"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="191"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="237"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="282"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="323"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="369"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="403"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="498"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="505"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR23151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="561" mass="64087" checksum="DF16B89ED8F0FDE0" modified="2009-07-28" version="1">MLSSRHVRIAWARCGSLQLLTHLSRAHPRCCTPRTPLSPPPPPHSRLSRAMLRYAEDNTEGYQDGLETLLNLLRIEMGSDRWANCSVRMLNSGNLLLRNASTGQMCNRSSSSPSFFVWCADNSTLEYKALVDVLRNHTLPTFGVQIEKDGFSAGCLMASFILCIVCAGSWMLLLVLLLLPANNHNHRKKMVYLGVVYQAIWHTVILDRSMDTVFEEQYMGNYQNAGALYDNVMTSTLYSVMLFFATVISNLNWLDIVYYMFHNYRKTNSSWVPRLFNNRNKRIIAVGVALTCAQAVLKAVKLWGARRYVGTVDVVLRSVDFTIYTLFSLSVAYYVWHDFGFTLEPQKQQRARSWRALFVFIWNDYHETVLLLFYNAVVMASLYMCTILIMTMPRHVFTWMQSLISFLNVLVTVNTWGLIGVLERRERTLSKETVLGRKINNRDKFFVDPKVDYDHGDGLVVSDDLGLAPDAATVQNSVHRRFGLDPVLRPAKVLRSKLAAKRRRGENASRSRHEGSTFEIQSMRHSIGERRASADQDWAQGGDNESAETLLTRNYIYDHDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHE8</accession>
    <name>C5DHE8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">E3 ubiquitin ligase complex SCF subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0E03784g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Essential component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complexes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the SKP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553646.1">
        <property type="nucleotide sequence ID" value="XM_002553600.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHE8"/>
    <dbReference type="FunCoup" id="C5DHE8">
        <property type="interactions" value="934"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHE8"/>
    <dbReference type="GeneID" id="8291795"/>
    <dbReference type="KEGG" id="lth:KLTH0E03784g"/>
    <dbReference type="eggNOG" id="KOG1724">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059252_4_0_1"/>
    <dbReference type="InParanoid" id="C5DHE8"/>
    <dbReference type="OMA" id="DKYTASM"/>
    <dbReference type="OrthoDB" id="2342932at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18322">
        <property type="entry name" value="BTB_POZ_SKP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000133">
        <property type="entry name" value="Suppressor of kinetochore protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016897">
        <property type="entry name" value="SKP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001232">
        <property type="entry name" value="SKP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036296">
        <property type="entry name" value="SKP1-like_dim_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016072">
        <property type="entry name" value="Skp1_comp_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016073">
        <property type="entry name" value="Skp1_comp_POZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11165">
        <property type="entry name" value="SKP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01466">
        <property type="entry name" value="Skp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03931">
        <property type="entry name" value="Skp1_POZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028729">
        <property type="entry name" value="E3_ubiquit_lig_SCF_Skp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00512">
        <property type="entry name" value="Skp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81382">
        <property type="entry name" value="Skp1 dimerisation domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="SKP1 component POZ" evidence="6">
        <location>
            <begin position="61"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="SKP1 component dimerisation" evidence="5">
        <location>
            <begin position="135"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="39"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="49"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR028729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR23209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="185" mass="21314" checksum="E98845877DCA842F" modified="2009-07-28" version="1">MGKDKAQNVVLVSVEGERFTVERKIAERSLLLKNYLNDMQDNGLQSDGSDSEEEEDADDEDGDDEIVMPVPNVRSSVLQKVIEWAEHHKDSNFPDENDDDARKTAPADPWDREFLKVDQEMLYEIMQAANYLNIKPLLDAGCKVVAEMIRGRTPEEIRRTFNIVNDFTPEEEAAIRRENEWAEDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHI6</accession>
    <name>C5DHI6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 12">1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase</fullName>
            <ecNumber evidence="4 12">5.3.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 12">5-proFAR isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9 12">Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0E04620g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide = 5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:15469"/>
            <dbReference type="ChEBI" id="CHEBI:58435"/>
            <dbReference type="ChEBI" id="CHEBI:58525"/>
            <dbReference type="EC" id="5.3.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 4/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the HisA/HisF family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.16" evidence="4 12"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553684.1">
        <property type="nucleotide sequence ID" value="XM_002553638.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHI6"/>
    <dbReference type="FunCoup" id="C5DHI6">
        <property type="interactions" value="248"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHI6"/>
    <dbReference type="GeneID" id="8291833"/>
    <dbReference type="KEGG" id="lth:KLTH0E04620g"/>
    <dbReference type="eggNOG" id="KOG3055">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065050_0_0_1"/>
    <dbReference type="InParanoid" id="C5DHI6"/>
    <dbReference type="OMA" id="IEWNKTH"/>
    <dbReference type="OrthoDB" id="446074at2759"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00009"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003949">
        <property type="term" value="F:1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04723">
        <property type="entry name" value="HisA_HisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000110">
        <property type="entry name" value="1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011858">
        <property type="entry name" value="His6/HISN3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006062">
        <property type="entry name" value="His_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044524">
        <property type="entry name" value="Isoase_HisA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02129">
        <property type="entry name" value="hisA_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43090">
        <property type="entry name" value="1-(5-PHOSPHORIBOSYL)-5-[(5-PHOSPHORIBOSYLAMINO)METHYLIDENEAMINO] IMIDAZOLE-4-CARBOXAMIDE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43090:SF2">
        <property type="entry name" value="1-(5-PHOSPHORIBOSYL)-5-[(5-PHOSPHORIBOSYLAMINO)METHYLIDENEAMINO] IMIDAZOLE-4-CARBOXAMIDE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00977">
        <property type="entry name" value="His_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 11" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="8 12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU364022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR23247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="266" mass="29804" checksum="130600A28DD5A3F9" modified="2009-07-28" version="1">MTRFVGCIDLHNGQVKQIVGGTLKEDQKEDLKTNFVSQHSSSYYAKLYRDNEVSGCHVIKLGPNNDEAARLALASVPGFLQVGGGINYDNCQEWLEIASKVIVTSWLFNSNGEFQLANLEKISSRCGKDRLVVDLSCRKVEDGTSHKWVVAMNKWQKLTDLELNHDTFSRLSQYADEFLIHAADVEGLCRGVDEALVAKLYEWTEHLPHLKIVYAGGAKSIQDLALMNRLSHGKVDLTYGSSLDIFGGNLVAFKECCEWNHRDHAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHL5</accession>
    <name>C5DHL5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pescadillo homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Nucleolar protein 7 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">NOP7</name>
        <name evidence="8" type="ordered locus">KLTH0E05346g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the NOP7 complex, which is required for maturation of the 25S and 5.8S ribosomal RNAs and formation of the 60S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the NOP7 complex, composed of ERB1, NOP7 and YTM1. Within the NOP7 complex ERB1 appears to interact directly with NOP7 and YTM1. The NOP7 complex also associates with the 66S pre-ribosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
            <location evidence="5">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
            <location evidence="5">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the pescadillo family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553713.1">
        <property type="nucleotide sequence ID" value="XM_002553667.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHL5"/>
    <dbReference type="FunCoup" id="C5DHL5">
        <property type="interactions" value="1457"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHL5"/>
    <dbReference type="GeneID" id="8291866"/>
    <dbReference type="KEGG" id="lth:KLTH0E05346g"/>
    <dbReference type="eggNOG" id="KOG2481">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019619_1_1_1"/>
    <dbReference type="InParanoid" id="C5DHL5"/>
    <dbReference type="OMA" id="QKVTWIV"/>
    <dbReference type="OrthoDB" id="10264910at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070545">
        <property type="term" value="C:PeBoW complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043021">
        <property type="term" value="F:ribonucleoprotein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd17709">
        <property type="entry name" value="BRCT_pescadillo_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000067">
        <property type="entry name" value="Pescadillo homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03028">
        <property type="entry name" value="Pescadillo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010613">
        <property type="entry name" value="PES"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12221">
        <property type="entry name" value="PESCADILLO - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12221:SF6">
        <property type="entry name" value="PESCADILLO HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16589">
        <property type="entry name" value="BRCT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06732">
        <property type="entry name" value="Pescadillo_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="2 5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="BRCT" evidence="7">
        <location>
            <begin position="349"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="301"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="452"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="308"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="466"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="511"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="534"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="563"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="573"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="586"/>
            <end position="596"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR23276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="603" mass="67715" checksum="4557C2BE41749601" modified="2009-07-28" version="1">MRIKKKNVSGNARNFITRSQAVRKLQVSLADFRRLCIFKGIYPREPRNKKKANKGSTAPTTFYYFKDIQYLMHEPVLERFREHKTFSKKLTKALGRGEVSSAKKLEEKSPSYKLDHIIKERYPSFADALRDIDDALNMLFLFANLPASDQVSARVTKDAQVICNQWLAYVARERAVRKVFVSIKGVYYQASVKGEDIRWLVPYKFPENIPSDVDFRIMLTFLEFYSTLMHFVLYKLYTDSGLVYPPKLDAAKDKIIGGLSAYILESNAEDSSLTAPQLADSNSDAKALDAKTLSSAIKADASEKAADDAEDAGGEDAEDELGEGELDTFEDKNKNQGDLLIQPSKYDSPTATLFSDFVFYISREVPVDILEFLVLACGGSVICEAALDQLESKKDIDLGKVTHQIVDRPVLKNKVGGRTYIQPQWVFDCINKGELVPANLYLPGETLPPHLSPWGDAAGYDPTAEPAEDESGDESEDVSDQEAGAESDDAEASAEAAAEDAAEEESDEDLEAQKELELEAKGIPYSDAKDKKEVKKKSKKPEKRKATEEDEEKDLKTIMMSNKQRKLYKKMKYSNEKKDDRVEKLKQKKKAIAKAKSKLEKTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHX0</accession>
    <name>C5DHX0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0E07788p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0E07788g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The proteasome degrades poly-ubiquitinated proteins in the cytoplasm and in the nucleus. It is essential for the regulated turnover of proteins and for the removal of misfolded proteins. The proteasome is a multicatalytic proteinase complex that is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. It has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the peptidase T1A family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553818.1">
        <property type="nucleotide sequence ID" value="XM_002553772.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHX0"/>
    <dbReference type="FunCoup" id="C5DHX0">
        <property type="interactions" value="1139"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHX0"/>
    <dbReference type="MEROPS" id="T01.971"/>
    <dbReference type="GeneID" id="8291975"/>
    <dbReference type="KEGG" id="lth:KLTH0E07788g"/>
    <dbReference type="eggNOG" id="KOG0182">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035750_4_1_1"/>
    <dbReference type="InParanoid" id="C5DHX0"/>
    <dbReference type="OMA" id="YGYDMPV"/>
    <dbReference type="OrthoDB" id="431557at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010499">
        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03754">
        <property type="entry name" value="proteasome_alpha_type_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000026">
        <property type="entry name" value="Proteasome subunit alpha type-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034642">
        <property type="entry name" value="Proteasome_subunit_alpha6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 7" id="KW-0647">Proteasome</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="8">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR23381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="246" mass="27601" checksum="89A30FE71DBF166A" modified="2009-07-28" version="1">MSAAGYDRHITIFSPEGRLYQVEYAFKATNQTNINSMAIRGNDCCVVINQKKVPDKLLDPSTMSYLFPISKHIGMVANGPIPDARNAALRAKAEVAEYRYKYGYDMPADVLAKRMANLSQIYTQRAYMRPLGVILTFVSVDEELGPSIYKCDPAGYYVGYKATATGPKQQELTSALEKYFKKRKSDCVNEDSYEKVVELGITHMIDALGTEFSKKDLEIGVALKDKFFCLTPDQIEERLVAIAEQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIB7</accession>
    <name>C5DIB7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0E11242p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0E11242g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553965.1">
        <property type="nucleotide sequence ID" value="XM_002553919.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIB7"/>
    <dbReference type="FunCoup" id="C5DIB7">
        <property type="interactions" value="21"/>
    </dbReference>
    <dbReference type="GeneID" id="8292131"/>
    <dbReference type="KEGG" id="lth:KLTH0E11242g"/>
    <dbReference type="eggNOG" id="ENOG502S7GU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064984_0_0_1"/>
    <dbReference type="InParanoid" id="C5DIB7"/>
    <dbReference type="OMA" id="INHLFHA"/>
    <dbReference type="OrthoDB" id="4038608at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR23528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="364" mass="43149" checksum="020940C4B2B96192" modified="2009-07-28" version="1">MGYVTAGYPSRPHKRVKKQERRVYYDRKRLKVNEIDERRDLQLRDRLRGPASLQLLPTDVLERIFTLAGRENSMPVLNRFFQRCLRPTRYLVSRFILENYTQDLNSLLPRDSPVLLVLLDVTFRNGTLLQFLNENPQFLDCVHDAVVSPDQADELSRERRILLEQGRLEDINALHWQTEMHPESNKPMREFPAAFYQRPALFFENEISKRPVTYNQLLLRLQSSYAIQQPAWVCDLLIEWFFLENENFDINHLFHAVNLVLHLSVNDRPRFEDVSPLTKLITYMYLDVTDHLLQLLLCENLEDVGLIRERKVKIVNKFIRKFYRGSTELLSTDELWMTVHEVKDRRLVESISDYGGKPSFNVVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIF7</accession>
    <name>C5DIF7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0E12166p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0E12166g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554005.1">
        <property type="nucleotide sequence ID" value="XM_002553959.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIF7"/>
    <dbReference type="FunCoup" id="C5DIF7">
        <property type="interactions" value="28"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIF7"/>
    <dbReference type="ESTHER" id="lactc-c5dif7">
        <property type="family name" value="Steryl_acetyl_hydrolase"/>
    </dbReference>
    <dbReference type="GeneID" id="8292173"/>
    <dbReference type="KEGG" id="lth:KLTH0E12166g"/>
    <dbReference type="eggNOG" id="KOG1515">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067868_0_0_1"/>
    <dbReference type="InParanoid" id="C5DIF7"/>
    <dbReference type="OMA" id="YGKVFTR"/>
    <dbReference type="OrthoDB" id="2152029at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050300">
        <property type="entry name" value="GDXG_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019436">
        <property type="entry name" value="Say1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081">
        <property type="entry name" value="AB HYDROLASE SUPERFAMILY PROTEIN C4A8.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081:SF8">
        <property type="entry name" value="ALPHA_BETA HYDROLASE FOLD-3 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10340">
        <property type="entry name" value="Say1_Mug180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="328" mass="37209" checksum="12E724D8D22C0E85" modified="2009-07-28" version="1">MIEPIIFFSKLAFRLPYSLVKAWVGGSLAELGSSKAVARVFLSEFAENLDPVTLRWVVNPVLRSVNVLSLWWLGAAGSDGRLRWLYKEEGAPVLLYVHGGGMCVDMFACSLGYLKLLKKEIGHLSIAIADYSLCARYPQAIEDVWTEYKKIIDEHKEVWLMGDSAGGNLGMNILQRCASDYTRFPEKCVLISPWLNVTKSETSFAHPGTDDYLTRNQLAMFKNVYAPNGSHQDPFLNIEANFDDGLWTTIMRHTKFIVLCGSDEILCPEIIRLCSKLAKIDEDRVKMCMEPRGVHCNTLLFDAWDPYAEHSSFPEILRFLRSDFYEKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIM4</accession>
    <name>C5DIM4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0E13662p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0E13662g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554072.1">
        <property type="nucleotide sequence ID" value="XM_002554026.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIM4"/>
    <dbReference type="FunCoup" id="C5DIM4">
        <property type="interactions" value="1348"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIM4"/>
    <dbReference type="GeneID" id="8292241"/>
    <dbReference type="KEGG" id="lth:KLTH0E13662g"/>
    <dbReference type="eggNOG" id="KOG0103">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005965_5_1_1"/>
    <dbReference type="InParanoid" id="C5DIM4"/>
    <dbReference type="OMA" id="WEQSPEI"/>
    <dbReference type="OrthoDB" id="434160at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.30:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000171">
        <property type="entry name" value="Heat shock 70 kDa protein 4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000004">
        <property type="entry name" value="Heat shock 70 kDa protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000020">
        <property type="entry name" value="Heat shock SSE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639:SF4">
        <property type="entry name" value="HSC70CB, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639">
        <property type="entry name" value="HSC70CB, ISOFORM G-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0346">Stress response</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="653"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="669"/>
            <end position="687"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR23635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="694" mass="77353" checksum="4368A9F5193ADF2A" modified="2009-07-28" version="1">MSTPFGLDLGNNNSVLAVARNRGIDIVVNEVSNRSTPSLVGFGQKNRFLGEAGKNKQISNIKNTVESLKRIIGLDYNDPDFEKEAKYFQAKLVKLENGKIGAQVRLAGEQATFSATQLVAMFINKVKNTVVDETKANITDVAIAVPAWYTEEQRYAVADAARIAGLNPVRVVNDVTAAAVTYGVFKSEMPEGEQKPRIVAFVDIGHSTYTCSIGAFKRGELKILGTAYDKHFGGRDFDRAITEHFADEFKTKYKIDIRENAKAYSRVMTGAERLKKVLSANTAAPFSVESVMNDIDVSSQLTREELEQLVKPLLERVTDPVTKALAQANLKAEEVDFVEIVGGTTRIPCLKNAISEAFGKPLSATLNQDEAIAKGAAFICAIHSPTMRVRPFKFEDIHPYSVSYFWDKQEEDEDNLEVFPARSAFPSTKMITLHRTGDFTMKAKFTNSKELPAGLPTEIAKWEITGVQIPEGQTSVPVKLKLRCDPSGLHTIESAYSVEDVKVQEEVPRAEDAPEDTEPEYREVTKTVKKDDLTIVAHTFALPAEELNALIEKENELFSQDKLVAETEDRKNALEEYIYTLRGKLDEEYAPFASDDEKAKLRDMLAKAEDWLYDDGYDSTKARYIAKYEELASLGNMIRGRYLAKEEEKRQALRQKDEAAKMAAMAEKMAAERKAQAEKQNEAKPDAEGDIDLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIR5</accession>
    <name>C5DIR5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein STU1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0E14586g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CLASP family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554113.1">
        <property type="nucleotide sequence ID" value="XM_002554067.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DIR5">
        <property type="interactions" value="177"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIR5"/>
    <dbReference type="GeneID" id="8292283"/>
    <dbReference type="KEGG" id="lth:KLTH0E14586g"/>
    <dbReference type="eggNOG" id="ENOG502QT5T">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_256206_0_0_1"/>
    <dbReference type="InParanoid" id="C5DIR5"/>
    <dbReference type="OMA" id="TFWYYYK"/>
    <dbReference type="OrthoDB" id="46159at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005881">
        <property type="term" value="C:cytoplasmic microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990023">
        <property type="term" value="C:mitotic spindle midzone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005876">
        <property type="term" value="C:spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060172">
        <property type="term" value="P:astral microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090307">
        <property type="term" value="P:mitotic spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024395">
        <property type="entry name" value="CLASP_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21567">
        <property type="entry name" value="CLASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21567:SF9">
        <property type="entry name" value="CLIP-ASSOCIATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12348">
        <property type="entry name" value="CLASP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CLASP N-terminal" evidence="8">
        <location>
            <begin position="279"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="513"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="952"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="527"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="557"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="585"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="958"/>
            <end position="974"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR23676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1365" mass="153524" checksum="49DCAE8AD894B20C" modified="2009-07-28" version="1">MANVRTFPLYDVLMAASTSDEQKLQQLSEFKGHVKKELVDERAVPAYFECLCHTLNIHLKNGRTGSRVFTLCHSTLCYLIKRVAMQSPAQFDAATTHSLLVPLLQTVNSEKKVWSGSMKALEAIYLAQPSVLEEALEKLQLRESDRVATLLVIDELVQLHQKNNRNPLEILNKFTALFLSILNQPKHSLSQGCELIRDILAKYYTMQSMQDFARRIRAPESRALFDVKPELPIEEVYVDSNPKPQQSIFSVGAELQSLFKDQAKSSPTQPKNYSSPEALRKDLENLVAPFASHKETEHNWRQRQEAIITMRGAVAGNAVDEYSEDLIALFKELQLSDCISKAVSSLRTSLSIHGCSLVKEMAGRLQEKIDPLAEGLFNSLRSVLSATKKLASQNAFFAACSLLAAMQFHNRIFQSCFMLSKDKNVSPRCFGAVFLRIFLIRFHSKLDNGLVYIDEWVHRSITDAQTKVRESMRTTFWYYYKAYPTNAKRLLDGLLPHFRRAVEASIPPNLDINYSMGNTKSSDSSRRSSMGPSRTPSYAGPTQSSHLQRLSALRSASDYQVPSNRAAQASFSARKTSGPAGLTSKPESVASQPFQGPINELSAQIDLTAEITQNNSNTLIKKYMQDSRSVQDKQKNAQSELKDVIHYLSSQNLSENKMGLQLLQNVILVGVPLQKQEISPLLRKIMLRDPQCLESLLRIPKFLDLLTVSQTIEAFAINRLPCTGLTERLTVEVLIEGVNALLADAYPSERENSFYYVKFRQTLFDYSFDLLTDICKYAKGLSNSSFGATVSKLFNLYGNDLDLQKYFDTLFHLFSHNRVVFSDLLGKSPVFLKTKIGRELEKRDPTLHFDFLAQHVPMSDIPLEDEKLYTELTMVQPSTVNQRSSSAESVLIHEIEGQNNQEMPNIETEPADENLEEVQGFTKFGGLSKLTEMTRVVSLYEKHNDQIEVSGKSALDTDGDHRMSSEGLENKSETPDVDLSDIFSEGRGKKVNSVKFHDNPSIIQQTGTSCCGVKKLESSNDLETISSNNSDLDTVGTIEAKSDHENSQSDPAGQEFEERAWSMHIAARNSPEKGAIGSAAVKTCSKKEDIPRRKIDFLALASKWPLLKFELEAIIADLGIPFRSESMASIINKIKSGSFTLKDLNLMIGFILVSVQQDTLENWFAQPNAFTELTEVQETLLESIHNSLKFPEALAVRCLLLCSCLVLMELESWEETGDQNQGMTDIAWASILSIVDRAEAFTSESFLLCEDLRNILLFRQHMERAQVKELIARLSLSENMSTVKKTFLLASSRIVLETHVDLIDIALLEEISKNAIPYVFDERTELRKESLELIGILWDLSSKQKQHVQTLDQIPEGLMKLIMSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIS3</accession>
    <name>C5DIS3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">NADPH-dependent diflavin oxidoreductase 1</fullName>
            <ecNumber evidence="10">1.18.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">NADPH-dependent FMN and FAD-containing oxidoreductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">TAH18</name>
        <name evidence="13" type="ordered locus">KLTH0E14762g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">NADPH-dependent reductase which is a central component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Transfers electrons from NADPH via its FAD and FMN prosthetic groups to the [2Fe-2S] cluster of DRE2, another key component of the CIA machinery. In turn, this reduced cluster provides electrons for assembly of cytosolic iron-sulfur cluster proteins. Positively controls H(2)O(2)-induced cell death.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>2 oxidized [2Fe-2S]-[protein] + NADPH = 2 reduced [2Fe-2S]-[protein] + NADP(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:67716"/>
            <dbReference type="Rhea" id="RHEA-COMP:17327"/>
            <dbReference type="Rhea" id="RHEA-COMP:17328"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 10">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with DRE2; as part of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Mitochondrion</location>
        </subcellularLocation>
        <text evidence="10">Relocalizes to mitochondria after H(2)O(2) exposure.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the NADPH-dependent diflavin oxidoreductase NDOR1 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">In the N-terminal section; belongs to the flavodoxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.18.1.-" evidence="10"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554121.1">
        <property type="nucleotide sequence ID" value="XM_002554075.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIS3"/>
    <dbReference type="FunCoup" id="C5DIS3">
        <property type="interactions" value="783"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIS3"/>
    <dbReference type="GeneID" id="8292291"/>
    <dbReference type="KEGG" id="lth:KLTH0E14762g"/>
    <dbReference type="eggNOG" id="KOG1159">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_17_6_1"/>
    <dbReference type="InParanoid" id="C5DIS3"/>
    <dbReference type="OMA" id="DIMSIPR"/>
    <dbReference type="OrthoDB" id="1856718at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160246">
        <property type="term" value="F:NADPH-iron-sulfur [2Fe-2S] protein oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.360:FF:000056">
        <property type="entry name" value="NADPH-dependent diflavin oxidoreductase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.80:FF:000030">
        <property type="entry name" value="NADPH-dependent diflavin oxidoreductase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.990.10">
        <property type="entry name" value="NADPH-cytochrome p450 Reductase, Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03178">
        <property type="entry name" value="NDOR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003097">
        <property type="entry name" value="CysJ-like_FAD-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001094">
        <property type="entry name" value="Flavdoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023173">
        <property type="entry name" value="NADPH_Cyt_P450_Rdtase_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028879">
        <property type="entry name" value="NDOR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384:SF10">
        <property type="entry name" value="NADPH-DEPENDENT DIFLAVIN OXIDOREDUCTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384">
        <property type="entry name" value="NITRIC OXIDE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00667">
        <property type="entry name" value="FAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00258">
        <property type="entry name" value="Flavodoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00369">
        <property type="entry name" value="FLAVODOXIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 10" id="KW-0274">FAD</keyword>
    <keyword evidence="4 10" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5 10" id="KW-0288">FMN</keyword>
    <keyword evidence="9 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7 10" id="KW-0521">NADP</keyword>
    <keyword evidence="8 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="11">
        <location>
            <begin position="6"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="12">
        <location>
            <begin position="220"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="12"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="59"/>
            <end position="62"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="106"/>
            <end position="115"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="374"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="404"/>
            <end position="407"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="436"/>
            <end position="439"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="529"/>
            <end position="530"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="538"/>
            <end position="542"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="614"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR23684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="614" mass="70377" checksum="9BCB7447A49B1078" modified="2009-07-28" version="1">MSSKKIAILYGSETGNSFEFASILSYKLHRQHFAHTLSSFGNYNARDILSCRYMFIICSTTGQGDLPRNAKENVSGEPRGTLWSFLKRRDLPANFLSHLHVTMLGLGDSSYPKFNFAIRKFHKRMVNQLGANEIFPRLEADESGIIGSNGGTGTGVEAVYFEYEKRIVQYLAGKFPTWKLNNQVIKREEIPAESFMAPKCVLKLSTNSNALEGPSFAAPSSIKTGSVVTNKRITHEDHFQDVRQFVFRGESEFYEPGDTVALYPHNKDEDVQKFLEAQPHWKEYADKALELSGPMIDQEGGFVSPMTLRNLLKFHCEITSIPRKTFFMKTWMFATDKSRLEGGEEQLIQQRNKLSQFALEEDMDDLYDYCNRPRRSLLEVLGDFPSLKLPWEYALEYLPHIKPRLFSISSKPLDPEIELTVAIVKYKTILRKIRKGLCTDYLQALEQGDTVRYKLQRNQLLKPWMKSLPAVLISPGVGLAPMKCLIQSEFFGQIALFFGNRVKERDFLYENELTAWHKSGKIALYTCFSRDPLHSPKARYVQDVMWQNGEKLADLILNGKAIVYICGSSGKMPVQVRLTIIEILKEWGGLTENNVAEDYLKNMEKDDRYLQETW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIX9</accession>
    <name>C5DIX9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0E16016p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0E16016g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554177.1">
        <property type="nucleotide sequence ID" value="XM_002554131.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIX9"/>
    <dbReference type="FunCoup" id="C5DIX9">
        <property type="interactions" value="1318"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIX9"/>
    <dbReference type="GeneID" id="8292348"/>
    <dbReference type="KEGG" id="lth:KLTH0E16016g"/>
    <dbReference type="eggNOG" id="KOG2584">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015572_4_0_1"/>
    <dbReference type="InParanoid" id="C5DIX9"/>
    <dbReference type="OMA" id="SRLHVCH"/>
    <dbReference type="OrthoDB" id="10258955at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004038">
        <property type="term" value="F:allantoinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006145">
        <property type="term" value="P:purine nucleobase catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050138">
        <property type="entry name" value="DHOase/Allantoinase_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002195">
        <property type="entry name" value="Dihydroorotase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018228">
        <property type="entry name" value="DNase_TatD-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43668">
        <property type="entry name" value="ALLANTOINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43668:SF2">
        <property type="entry name" value="ALLANTOINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00482">
        <property type="entry name" value="DIHYDROOROTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01137">
        <property type="entry name" value="TATD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="4">
        <location>
            <begin position="63"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Amidohydrolase-related" evidence="4">
        <location>
            <begin position="323"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR23740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="542" mass="59486" checksum="760CBB286E5555FD" modified="2009-07-28" version="1">MPVKAIASRSVIIDHQCIPATIVYSTDSGKIVEIYKNEVIRGIHDERLESHKVVDYGIVTPHVIMPGLVDSHVHLNEPGRTEWEGFATGTQAASSGGVTTVVDMPLNAVPPTTTVKNLNIKLNAAQNQMWCDVAFWGGLVPSNLDDLIPLVRAGVRGFKGFLLDSGVDEFPQVSKKYIMNALEVLKNEKTQLLFHAELPPDHNFGILEPSREPMDLEAVRSVESAKNLTNNEIRALAESSVLSSAEPRYGQPSHIVHHDDLISPPLTVAAGVDDTLANVDPTEYGSFLASRPDQCETSAIGLLISCLRESLALFQSAPPVHIVHLATQEAIPMIIKAQRELGLPITVETCFHYLTFAAEQIPAKATQFKCCPPIRTEDNRKALWKALHDRIITTVVSDHSPCTPQLKNLSEGDFFGAWGGISSVGLGLSILITEGSKMSPPVSLTDIAEWCCENTAKQVGLHDRKGFLRTGYDADFVVLDQHQRRTISKDNILYKNRLTAYEGRELKGQVMSTFLRGQLIFEATRGLSSIPKGRTLLEPRAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJ03</accession>
    <name>C5DJ03_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0E16566p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0E16566g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 4">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the trans-sulfuration enzymes family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554201.1">
        <property type="nucleotide sequence ID" value="XM_002554155.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJ03"/>
    <dbReference type="FunCoup" id="C5DJ03">
        <property type="interactions" value="198"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJ03"/>
    <dbReference type="GeneID" id="8292373"/>
    <dbReference type="KEGG" id="lth:KLTH0E16566g"/>
    <dbReference type="eggNOG" id="KOG0053">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018986_3_0_1"/>
    <dbReference type="InParanoid" id="C5DJ03"/>
    <dbReference type="OMA" id="HKKMHGV"/>
    <dbReference type="OrthoDB" id="3512640at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016846">
        <property type="term" value="F:carbon-sulfur lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000072">
        <property type="entry name" value="Putative cystathionine beta-lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054542">
        <property type="entry name" value="Cys_met_metab_PP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808:SF35">
        <property type="entry name" value="CYSTATHIONINE GAMMA-SYNTHASE (AFU_ORTHOLOGUE AFUA_7G01590)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808">
        <property type="entry name" value="TRANS-SULFURATION ENZYME FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001434">
        <property type="entry name" value="CGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00868">
        <property type="entry name" value="CYS_MET_METAB_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="3">
        <location>
            <position position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR001434-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR23764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="381" mass="42344" checksum="CFF2A6174D5B244A" modified="2009-07-28" version="1">MPFSSTATIHGDDRLNRVSDVAPPINVSTTFRYDDEHLEPATISTGTHTSDSPMIYSRLGHPNADRLEVVLTEILDGPTVVYASGLAAYHAILIHVNPKRLFQPHCYHGCEAVAKIISRISGMEILPLENLEDKCQSGDLVHLEDPGNPYGEVVNLDEFAEKIHRAGALMLVDSTLAPPPLRNTWEFGADIVMHSGTKYFGGHSDLLSGIVSVRDEKVAQQLRNDRTYLGTIPASLESYLLLRSLRTMEIRVLKQSSNCTRLVEYLASHKDRYNSVVKDIRHASLQKEEFVKKQLPGGYGPVFSLLLFSQEQCKLLVKNTVLFQHATSLGGAESLIEWRAMSDPFANRNLIRVSVGLENAEDLIEDLAGTLEKIEKGDLTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJA9</accession>
    <name>C5DJA9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0F14938p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0F14938g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554835.1">
        <property type="nucleotide sequence ID" value="XM_002554789.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJA9"/>
    <dbReference type="FunCoup" id="C5DJA9">
        <property type="interactions" value="38"/>
    </dbReference>
    <dbReference type="GeneID" id="8293064"/>
    <dbReference type="KEGG" id="lth:KLTH0F14938g"/>
    <dbReference type="eggNOG" id="ENOG502S2RD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112115_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJA9"/>
    <dbReference type="OrthoDB" id="3992052at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014804">
        <property type="entry name" value="Pet20-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08692">
        <property type="entry name" value="Pet20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="72"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="182"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="80"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="182"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR24398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="229" mass="25363" checksum="0C89A37BBC24DB37" modified="2009-07-28" version="1">MAHKRQFSLEASKMLKRKSSEIFRSLPKVPTTQYLEPAELTRDILYSGYRPVIYPVKENPLFKEAGWKTQRLRGERGARKSSTVPQSSEMNAMAGPLGTGGIGSGGVNGTWRYNPRIPSKLLPYNLWSCTSMAMEYHPEWLAVPRTVVNKLRPFESPHRPVAERSTDCKAGSSPTVTAAAVAPATALPATGARAKAPARKRKPAEHGEDNKLSKQVGEFINYLREKDRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJU6</accession>
    <name>C5DJU6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">KLTH0F19228p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0F19228g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RING-box family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555022.1">
        <property type="nucleotide sequence ID" value="XM_002554976.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJU6"/>
    <dbReference type="FunCoup" id="C5DJU6">
        <property type="interactions" value="1128"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJU6"/>
    <dbReference type="GeneID" id="8293258"/>
    <dbReference type="KEGG" id="lth:KLTH0F19228g"/>
    <dbReference type="eggNOG" id="KOG2930">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115512_2_1_1"/>
    <dbReference type="InParanoid" id="C5DJU6"/>
    <dbReference type="OMA" id="NACPLDN"/>
    <dbReference type="OrthoDB" id="8962942at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031463">
        <property type="term" value="C:Cul3-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16485">
        <property type="entry name" value="mRING-H2-C3H2C2D_RBX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000273">
        <property type="entry name" value="E3 ubiquitin-protein ligase RBX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051031">
        <property type="entry name" value="RING-box_E3_Ubiquitin_Ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024766">
        <property type="entry name" value="Znf_RING_H2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11210">
        <property type="entry name" value="RING BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12678">
        <property type="entry name" value="zf-rbx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="12">
        <location>
            <begin position="44"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR24585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="110" mass="12787" checksum="B3B551E024C59705" modified="2009-07-28" version="1">MSEDRMDLDEPDVVQSEPVPKKRFEVKKWTAVAFWSWDIAVENCAICRNHIMEPCIQCQPTAMTDTDNECVAAWGACNHAFHLHCINKWLQTRNACPLDNQTWQFAKYGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5DK27</accession>
    <name>C5DK27_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0F01276p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0F01276g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>1-chloro-2,4-dinitrobenzene + glutathione = 2,4-dinitrophenyl-S-glutathione + chloride + H(+)</text>
            <dbReference type="Rhea" id="RHEA:51220"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
            <dbReference type="ChEBI" id="CHEBI:34718"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:133977"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 glutathione + H2O2 = glutathione disulfide + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:16833"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58297"/>
            <dbReference type="EC" id="1.11.1.9"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554265.1">
        <property type="nucleotide sequence ID" value="XM_002554219.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DK27"/>
    <dbReference type="FunCoup" id="C5DK27">
        <property type="interactions" value="749"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DK27"/>
    <dbReference type="GeneID" id="8292456"/>
    <dbReference type="KEGG" id="lth:KLTH0F01276g"/>
    <dbReference type="eggNOG" id="KOG1752">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026126_7_2_1"/>
    <dbReference type="InParanoid" id="C5DK27"/>
    <dbReference type="OMA" id="YCHKARA"/>
    <dbReference type="OrthoDB" id="418495at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015038">
        <property type="term" value="F:glutathione disulfide oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004602">
        <property type="term" value="F:glutathione peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03419">
        <property type="entry name" value="GRX_GRXh_1_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000026">
        <property type="entry name" value="Glutaredoxin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011767">
        <property type="entry name" value="GLR_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011899">
        <property type="entry name" value="Glutaredoxin_euk/vir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014025">
        <property type="entry name" value="Glutaredoxin_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02180">
        <property type="entry name" value="GRX_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45694">
        <property type="entry name" value="GLUTAREDOXIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45694:SF18">
        <property type="entry name" value="GLUTAREDOXIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00160">
        <property type="entry name" value="GLUTAREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00195">
        <property type="entry name" value="GLUTAREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Glutaredoxin" evidence="8">
        <location>
            <begin position="48"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR23828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="138" mass="15497" checksum="4070464B76024392" modified="2009-07-28" version="1">MEYSRTLTLVALVFTLFIIRRFFGSGFSKMASPQTVQHVRSLIKDNKIFVAAKTYCPYCNATLKLLFQDKKLRKDQVLLLQLDTMKEGSEIQEALTEISGQRTVPNIYILGEHIGGNSDLQALEASGKLDELLEKALA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5DK49</accession>
    <name>C5DK49_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0F01760p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0F01760g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PA-phosphatase related phosphoesterase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554287.1">
        <property type="nucleotide sequence ID" value="XM_002554241.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DK49"/>
    <dbReference type="FunCoup" id="C5DK49">
        <property type="interactions" value="79"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DK49"/>
    <dbReference type="GeneID" id="8292478"/>
    <dbReference type="KEGG" id="lth:KLTH0F01760g"/>
    <dbReference type="eggNOG" id="KOG3030">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021458_4_0_1"/>
    <dbReference type="InParanoid" id="C5DK49"/>
    <dbReference type="OMA" id="YGLDICQ"/>
    <dbReference type="OrthoDB" id="10030083at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008195">
        <property type="term" value="F:phosphatidate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046839">
        <property type="term" value="P:phospholipid dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03390">
        <property type="entry name" value="PAP2_containing_1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043216">
        <property type="entry name" value="PAP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165">
        <property type="entry name" value="LIPID PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165:SF155">
        <property type="entry name" value="LIPID PHOSPHATE PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="70"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="129"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="244"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="294"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="7">
        <location>
            <begin position="179"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR23850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="327" mass="36153" checksum="B00EBD5A760C5626" modified="2009-07-28" version="1">MPHGVRTAQQSKLHKSRDCSFKRYSRLPTRRAYARDKGAARTQITTPHHAFPQPQPPLNPSNMPPSLKSAVAYVAGFVPLYAACVVLIAALGFSEFHMAPRLSRQVRTDDPAIKKSFVAHETVTPVQCLFLAFPLPVLVLAGWCTVGSDSLVSRYQRRSFARLARPDWVPAKFHLFHISCVALALALGINGVLTNGFKLLLSNARPDFLARCQPADPLALYVSVAQCTQPDKLVLYEGLKSTPSGHSSFAVAGLGFLYLWLARHTCVSRLRHVWCPLLCVFVMASRVVDHRHHWYDVLTGGALGLAVCVGVWRTLMREEPQANLLPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DK72</accession>
    <name>C5DK72_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0F02288p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0F02288g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554310.1">
        <property type="nucleotide sequence ID" value="XM_002554264.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DK72"/>
    <dbReference type="STRING" id="559295.C5DK72"/>
    <dbReference type="GeneID" id="8292502"/>
    <dbReference type="KEGG" id="lth:KLTH0F02288g"/>
    <dbReference type="eggNOG" id="KOG1153">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011263_1_0_1"/>
    <dbReference type="InParanoid" id="C5DK72"/>
    <dbReference type="OMA" id="AMDWAVK"/>
    <dbReference type="OrthoDB" id="206201at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04077">
        <property type="entry name" value="Peptidases_S8_PCSK9_ProteinaseK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034193">
        <property type="entry name" value="PCSK9_ProteinaseK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050131">
        <property type="entry name" value="Peptidase_S8_subtilisin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806">
        <property type="entry name" value="PEPTIDASE S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806:SF13">
        <property type="entry name" value="SUBTILASE-TYPE PROTEINASE RRT12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0720">Serine protease</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002948787" evidence="6">
        <location>
            <begin position="17"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="7">
        <location>
            <begin position="127"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="136"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="169"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR23873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="380" mass="39631" checksum="2BF4934CD857B0B8" modified="2009-07-28" version="1">MRLFMAALFGLRAAGAVRFVAELGDTASVEEVLGEQYAATVAGGGVGAITLGNSFRAIYGEFPQQLLEDFYRSGQIVAMSMDRPLGVAEYMVQQHAPNHLARLSQKSSLRGGNGSDNGAYIYHSNAGNGVDVYLLDTGIDGTHPAFEGRVQKAADFSSDPVATGDPHGHGTAVAGVIGSSVFGVAKKCNLFDVRVANSTGHASLIGVLRALEHASKLAAVTKRPSLITIPLEMPRNTILNSAVEAVVRDLSIPVVVAAGNENRSACSVSPAGAYGALTIGSIDVARNDALAPFTNFAECVDLFTAGVDVATLGLDHSSEHHLSGTSISAGVASGLVAYYMSIGHYGMDAVNKIKLLSLPNVIPNLQQRSPETRNAILQNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKB4</accession>
    <name>C5DKB4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0F03234p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0F03234g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Additional factor that functions in concert with eIF-2 and the initiator tRNA in directing the ribosome to the proper start site of translation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SUI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554352.1">
        <property type="nucleotide sequence ID" value="XM_002554306.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKB4"/>
    <dbReference type="FunCoup" id="C5DKB4">
        <property type="interactions" value="795"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKB4"/>
    <dbReference type="GeneID" id="8292544"/>
    <dbReference type="KEGG" id="lth:KLTH0F03234g"/>
    <dbReference type="eggNOG" id="KOG1770">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082805_3_2_1"/>
    <dbReference type="InParanoid" id="C5DKB4"/>
    <dbReference type="OMA" id="CEFMITQ"/>
    <dbReference type="OrthoDB" id="10248435at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11566">
        <property type="entry name" value="eIF1_SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.780.10:FF:000005">
        <property type="entry name" value="Sui1 translation initiation factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.780.10">
        <property type="entry name" value="SUI1-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001950">
        <property type="entry name" value="SUI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036877">
        <property type="entry name" value="SUI1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005874">
        <property type="entry name" value="SUI1_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01160">
        <property type="entry name" value="SUI1_MOF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10388">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01253">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004499">
        <property type="entry name" value="SUI1_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55159">
        <property type="entry name" value="eIF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50296">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="SUI1" evidence="5">
        <location>
            <begin position="26"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00060093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR23915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="108" mass="12319" checksum="74E4939228EFD957" modified="2009-07-28" version="1">MSIENLKSFDPFADTGDNETATANYIHIRIQQRNGRKTLTTVQGVPAEYDLKRILKVLKKDFACNGNIVKDEEMGEIIQMQGDQRAKVCEFMITQLGMKKKNIKIHGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2023-06-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKC9</accession>
    <name>C5DKC9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0F03630p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0F03630g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554367.1">
        <property type="nucleotide sequence ID" value="XM_002554321.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKC9"/>
    <dbReference type="GeneID" id="8292561"/>
    <dbReference type="KEGG" id="lth:KLTH0F03630g"/>
    <dbReference type="HOGENOM" id="CLU_2333963_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKC9"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR23930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="98" mass="10991" checksum="ABAEDC9B41840940" modified="2009-07-28" version="1">MREISGSPRLSGPVGGRNWVLCEDDASPVWRTALELGAWAGMPASDVRLRHKNWGYAAPVQPRGWAEIVFEAIMVQTPPRVSFMVLCRIRISNLKIPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKD4</accession>
    <name>C5DKD4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Elongation of fatty acids protein</fullName>
            <ecNumber evidence="12">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0F03740g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a very-long-chain acyl-CoA + malonyl-CoA + H(+) = a very-long-chain 3-oxoacyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:32727"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:90725"/>
            <dbReference type="ChEBI" id="CHEBI:90736"/>
            <dbReference type="EC" id="2.3.1.199"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>an acyl-CoA + malonyl-CoA + H(+) = a 3-oxoacyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:50252"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:90726"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:50253"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the ELO family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="12"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554372.1">
        <property type="nucleotide sequence ID" value="XM_002554326.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKD4"/>
    <dbReference type="FunCoup" id="C5DKD4">
        <property type="interactions" value="667"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKD4"/>
    <dbReference type="GeneID" id="8292566"/>
    <dbReference type="KEGG" id="lth:KLTH0F03740g"/>
    <dbReference type="eggNOG" id="KOG3071">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048483_6_1_1"/>
    <dbReference type="InParanoid" id="C5DKD4"/>
    <dbReference type="OMA" id="PISWVPI"/>
    <dbReference type="OrthoDB" id="434092at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009922">
        <property type="term" value="F:fatty acid elongase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034625">
        <property type="term" value="P:fatty acid elongation, monounsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034626">
        <property type="term" value="P:fatty acid elongation, polyunsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019367">
        <property type="term" value="P:fatty acid elongation, saturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030457">
        <property type="entry name" value="ELO_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002076">
        <property type="entry name" value="ELO_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157:SF134">
        <property type="entry name" value="ELONGATION OF FATTY ACIDS PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157">
        <property type="entry name" value="FATTY ACID ACYL TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01151">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01188">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="6 12" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="3 12" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8 12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="71"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="132"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="198"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="232"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="273"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR23935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="342" mass="39369" checksum="BFF82E4179E43BAB" modified="2009-07-28" version="1">MLSTAQTYANEMIQKYPVLEQFQPTLDRPFFNISLWENFDTAMRYATNGKFIPSEFQFVPGKLPLSELPQVIAAITTYYVVIFGGRFLLQGSQPFQLNALFQLHNLFLTTLSLTLLVLMVEQLVPMIARNGLYYAICNIGAWTQPMVTLYYMNYITKYIEFIDTVFLVLKHKNLRFLHTYHHGATALLCYTQLVGTTAISWVVISLNLGVHVVMYWYYFLAARGIRVWWKEWVTRFQIMQFVLDIGFIYFAVYQKVSHLFFPQLPHCGDCVGSTTATFSGCAIISSYLFLFVAFYIEVYKRRGTKKSRIVKRVRGGVAAKVNEYVNVDVAHTSTPSPSPARK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKD9</accession>
    <name>C5DKD9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0F03850p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0F03850g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS11 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554377.1">
        <property type="nucleotide sequence ID" value="XM_002554331.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKD9"/>
    <dbReference type="FunCoup" id="C5DKD9">
        <property type="interactions" value="1179"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKD9"/>
    <dbReference type="GeneID" id="8292571"/>
    <dbReference type="KEGG" id="lth:KLTH0F03850g"/>
    <dbReference type="eggNOG" id="KOG0407">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072439_6_0_1"/>
    <dbReference type="InParanoid" id="C5DKD9"/>
    <dbReference type="OMA" id="KWGVAHI"/>
    <dbReference type="OrthoDB" id="1677536at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.80:FF:000002">
        <property type="entry name" value="40S ribosomal protein S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.80">
        <property type="entry name" value="Ribosomal protein S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01310">
        <property type="entry name" value="Ribosomal_uS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001971">
        <property type="entry name" value="Ribosomal_uS11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018102">
        <property type="entry name" value="Ribosomal_uS11_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036967">
        <property type="entry name" value="Ribosomal_uS11_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007176">
        <property type="entry name" value="PRK09607.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11759">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S14/30S RIBOSOMAL PROTEIN S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00411">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002131">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00054">
        <property type="entry name" value="RIBOSOMAL_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="128"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR23940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="137" mass="14648" checksum="70D80C71FF3CDF71" modified="2009-07-28" version="1">MIHLVQARDNSQVFGVARIYASFNDTFVHVTDLSGRETISRVTGGMKVKADRDESSPYAAMLAAQDVAAKCKEVGITALHVKIRATGGTKTKTPGPGGQAALRALARSGMRIGRIEDVTPVPSDSTRKKGGRRGRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKE7</accession>
    <name>C5DKE7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0F04070p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0F04070g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554385.1">
        <property type="nucleotide sequence ID" value="XM_002554339.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKE7"/>
    <dbReference type="STRING" id="559295.C5DKE7"/>
    <dbReference type="GeneID" id="8292580"/>
    <dbReference type="KEGG" id="lth:KLTH0F04070g"/>
    <dbReference type="eggNOG" id="KOG1404">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016922_4_0_1"/>
    <dbReference type="InParanoid" id="C5DKE7"/>
    <dbReference type="OrthoDB" id="10261433at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43094">
        <property type="entry name" value="AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43094:SF1">
        <property type="entry name" value="AMINOTRANSFERASE CLASS-III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR23948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="474" mass="51845" checksum="E1C82CDCDE2CBCDC" modified="2009-07-28" version="1">MTVSSAKSISKPSYNFQGTVDQTGPQVVGGKGVRIDVEKEGKVHENVLDAITGAAVGALGWGDEDALDIINKAARTSTYSYPCLISNKPSEALAKYYIDHSPEGAFSAALWCTSGSESNENALRIIRQYWLERGLPNKIKLMSRESSYHGFTLGAQSISWNPRVEPYAPYLMDREKINIQIPAAYTYRFKKDDETEEDYAKRLVDATEKKILENDPNTIAALTVETLPGSSLGTTPPPKGYLKGLRDLCNKYDIIFHLDEVMCGTGRSNPNGNFNCWENYLAPTDAPDIQTVGKTLGSGYVTIAGILIGPKIRSAYVEGSKSIHGGNTYSSHAFNCAVSLGIMQKLKNEKLTANIFKMGNLMGEKLSQILLSEDNIAGDVRGIGGFWSVEFVKDRATKEPFDLSLNIAGRFKAICFENNLNVMGMPGNPDGSCGDRCLLAPSFIITEKDVDEIVEIMAKSVLQLSSDLRKEGAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKL1</accession>
    <name>C5DKL1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ATP-dependent RNA helicase eIF4A</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Eukaryotic initiation factor 4A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ordered locus">KLTH0F05610g</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DEAD box helicase family. eIF4A subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554449.1">
        <property type="nucleotide sequence ID" value="XM_002554403.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKL1"/>
    <dbReference type="FunCoup" id="C5DKL1">
        <property type="interactions" value="1415"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKL1"/>
    <dbReference type="GeneID" id="8292647"/>
    <dbReference type="KEGG" id="lth:KLTH0F05610g"/>
    <dbReference type="eggNOG" id="KOG0327">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_1_0_1"/>
    <dbReference type="InParanoid" id="C5DKL1"/>
    <dbReference type="OMA" id="FGCQALV"/>
    <dbReference type="OrthoDB" id="10265785at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18046">
        <property type="entry name" value="DEADc_EIF4AII_EIF4AI_DDX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000089">
        <property type="entry name" value="Eukaryotic initiation factor 4A-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000031">
        <property type="entry name" value="Eukaryotic initiation factor 4A-III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044728">
        <property type="entry name" value="EIF4A_DEADc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 15" id="KW-0347">Helicase</keyword>
    <keyword evidence="4 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="18">
        <location>
            <begin position="22"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="16">
        <location>
            <begin position="53"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="17">
        <location>
            <begin position="234"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="14">
        <location>
            <begin position="22"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAR24012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="396" mass="44840" checksum="92A1571370B9422A" modified="2009-07-28" version="1">MSEGITDIEESQIQTNYDKVVYKFDDLNLKEELLRGIYGYGFVDPSAIQQRAILPITEGHDVLAQAQSGTGKTGTFSIAALQKIDPKIKAPQALMLAPTRELALQIQKVVMALAFHMDIKVHACIGGTSFVEDAEALRDGAQIVVGTPGRVFDMIERRRFRTDHIKMFILDEADEMLSSGFKEQIYKIFTLLPPTTQVVLLSATMPQDVLEVTTKFMRNPIRILVKKDELTLEGIKQFFINVEEEDYKYDCLSDLYDSISVTQAVIFCNTRRKVEELTQRLVADNFTVSAIYSDLPQQQRDTIMKEFRTGSSRILISTDLLARGIDVQQVSLVINYDLPTNKENYIHRIGRGGRFGRKGIAINFVTNKDVGAMRELERFYSTQIEELPANISELFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKL2</accession>
    <name>C5DKL2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">glycogenin glucosyltransferase</fullName>
            <ecNumber evidence="12">2.4.1.186</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">KLTH0F05632g</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Self-glucosylating initiator of glycogen synthesis. It catalyzes the formation of a short alpha (1,4)-glucosyl chain covalently attached via a glucose 1-O-tyrosyl linkage to internal tyrosine residues and these chains act as primers for the elongation reaction catalyzed by glycogen synthase. Capable of transferring glucosyl residues to unbound acceptors such as free oligoglucans or oligoglucan derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-tyrosyl-[glycogenin] + UDP-alpha-D-glucose = alpha-D-glucosyl-L-tyrosyl-[glycogenin] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23360"/>
            <dbReference type="Rhea" id="RHEA-COMP:14604"/>
            <dbReference type="Rhea" id="RHEA-COMP:14605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:140573"/>
            <dbReference type="EC" id="2.4.1.186"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>[1,4-alpha-D-glucosyl](n)-L-tyrosyl-[glycogenin] + UDP-alpha-D-glucose = [1,4-alpha-D-glucosyl](n+1)-L-tyrosyl-[glycogenin] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:56560"/>
            <dbReference type="Rhea" id="RHEA-COMP:14606"/>
            <dbReference type="Rhea" id="RHEA-COMP:14607"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:140574"/>
            <dbReference type="EC" id="2.4.1.186"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Vacuole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the glycosyltransferase 8 family. Glycogenin subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.186" evidence="12"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554450.1">
        <property type="nucleotide sequence ID" value="XM_002554404.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKL2"/>
    <dbReference type="FunCoup" id="C5DKL2">
        <property type="interactions" value="115"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKL2"/>
    <dbReference type="GeneID" id="8292648"/>
    <dbReference type="KEGG" id="lth:KLTH0F05632g"/>
    <dbReference type="eggNOG" id="KOG1950">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_549889_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKL2"/>
    <dbReference type="OMA" id="YHWSESA"/>
    <dbReference type="OrthoDB" id="2014201at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008466">
        <property type="term" value="F:glycogenin glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02537">
        <property type="entry name" value="GT8_Glycogenin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000148">
        <property type="entry name" value="Glg2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002495">
        <property type="entry name" value="Glyco_trans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050587">
        <property type="entry name" value="GNT1/Glycosyltrans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11183">
        <property type="entry name" value="GLYCOGENIN SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01501">
        <property type="entry name" value="Glyco_transf_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0320">Glycogen biosynthesis</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0464">Manganese</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0926">Vacuole</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950413" description="glycogenin glucosyltransferase" evidence="17">
        <location>
            <begin position="26"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="292"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="399"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="412"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00050886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00052293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00059480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAR24013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="496" mass="54849" checksum="B49A9F936E4ED282" modified="2009-07-28" version="1">MQEPRNRCGVITLMYSWAYLPGVLALGHQLQRALENSGHGFRTCVLVAKGLLEQKDASKQILLSVLESLYQEVIAVDPASTCSDSLLKLNKGNLELLHRPELAFTFMKLELWRLQQFERVVYLDCDTLLVSDAFWDILDVTKNQKRNEVGAVPDCGWPDMFNSGVLTIVPDLEIYAELAEYVMSTISVDGADQGVLNQYFNPNCRFGSRQSTIGGENGWIRLPFTYNVTVPNAGYQNAPAVHFFRSQIKLVHFIGRDKPWITRSQASDRTNDSYRDKWWQVYNEFLRKQTSEEPLGPVADHPGDAIQTGSDASKVSVKNPGTPPRPLIACQEALETSQQAPRTFPAAAEGTGQAPEPNTAAPLNPQARKEIPLSAPAIQPVTDADRGAHIQAALNALLGTSPTRALTEDPSTDPSETTGSNAHQELTPSAWDATKSPPPRDGPAEAPNFSFDTDYHWSESATLLEDDLPKLKLGPKPVFPWELYPDAPEVQRVFPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKX3</accession>
    <name>C5DKX3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0F08250p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0F08250g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554561.1">
        <property type="nucleotide sequence ID" value="XM_002554515.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKX3"/>
    <dbReference type="FunCoup" id="C5DKX3">
        <property type="interactions" value="134"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKX3"/>
    <dbReference type="GeneID" id="8292767"/>
    <dbReference type="KEGG" id="lth:KLTH0F08250g"/>
    <dbReference type="eggNOG" id="KOG3872">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031992_1_0_1"/>
    <dbReference type="InParanoid" id="C5DKX3"/>
    <dbReference type="OMA" id="RQDNGYF"/>
    <dbReference type="OrthoDB" id="687730at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22695">
        <property type="entry name" value="FHA_VPS64-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051176">
        <property type="entry name" value="Cent_Immune-Sig_Mod"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15715">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 170 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15715:SF37">
        <property type="entry name" value="LD47843P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FHA" evidence="3">
        <location>
            <begin position="68"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="72"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="386"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="293"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR24124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="432" mass="48035" checksum="22538568DD59A97C" modified="2009-07-28" version="1">MSGDTKPVVTRRTRSNSSSDGSASPMTQEFMAPSTAPRNRYTHIIILKSLNNTFETKFLVVPFKPDGLKLGRPIANSGAQPGGKQDLQSQVRPDNGHFDSRVLSRNHASLSCEPFSGKIYIRDLKSSNGTFVNGNRITQSDTELKVGDIIDLGTDIDAKFEHRKISALVEDISVIPLINEDEPFTNGAGKHSRDDLTGGLATPNAQRAAFEAAMFGDVNNLDLEESLLGSETEILSGIFINNSIGTSPNLINVIKTLVTEISLEKHEFEKMKSIERFLIHFTTNLEHINKLRVENNDLQLVQLQNSLKQKLNEKHDVAMQTHVDRLKKVEEENDSLRSDLEKQSKVEAKELKSLKREIEDLNTRLEVEKFKNTQLKKSADENSIYSKRLRDSGLNKAQDSPSKKSAGRKSTILLSAFTVGIAAAAFQYASKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKZ3</accession>
    <name>C5DKZ3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0F08690p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0F08690g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554581.1">
        <property type="nucleotide sequence ID" value="XM_002554535.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKZ3"/>
    <dbReference type="FunCoup" id="C5DKZ3">
        <property type="interactions" value="1056"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKZ3"/>
    <dbReference type="GeneID" id="8292787"/>
    <dbReference type="KEGG" id="lth:KLTH0F08690g"/>
    <dbReference type="eggNOG" id="KOG3336">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067902_1_0_1"/>
    <dbReference type="InParanoid" id="C5DKZ3"/>
    <dbReference type="OMA" id="YCPWNEK"/>
    <dbReference type="OrthoDB" id="407630at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006797">
        <property type="entry name" value="PRELI/MSF1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037365">
        <property type="entry name" value="Slowmo/Ups"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11158">
        <property type="entry name" value="MSF1/PX19 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04707">
        <property type="entry name" value="PRELI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50904">
        <property type="entry name" value="PRELI_MSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRELI/MSF1" evidence="1">
        <location>
            <begin position="1"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="231" mass="26328" checksum="EC6C347E0952D98B" modified="2009-07-28" version="1">MKLFQNTQEFSYPWEQVTAANWRKYPNEVSTHVVAVDVLRRELDSTGTRLTSERLITCQQSVPKWLAMLVGSSNKSYVREVSVVDLNAKTLTLRSCNLTCAHLLKVYETVKYQPHALDASRTVFQQEAQITAYATIQTLCNKIEEWSVKRFGENASKGRKGFDSVLRMLSDNWEQRDKLVDELSDSIADRVAGTVGEIRATTDSLIKETEMKSTSLVSRYSKETQRAFNKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DL90</accession>
    <name>C5DL90_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0F10956p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0F10956g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554678.1">
        <property type="nucleotide sequence ID" value="XM_002554632.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DL90"/>
    <dbReference type="FunCoup" id="C5DL90">
        <property type="interactions" value="35"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DL90"/>
    <dbReference type="GeneID" id="8292890"/>
    <dbReference type="KEGG" id="lth:KLTH0F10956g"/>
    <dbReference type="eggNOG" id="KOG2273">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007739_1_0_1"/>
    <dbReference type="InParanoid" id="C5DL90"/>
    <dbReference type="OMA" id="QLWQDPE"/>
    <dbReference type="OrthoDB" id="2117459at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047168">
        <property type="entry name" value="LEC1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024554">
        <property type="entry name" value="LEC1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024555">
        <property type="entry name" value="PX-associated"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47185">
        <property type="entry name" value="PX DOMAIN-CONTAINING PROTEIN YPR097W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47185:SF1">
        <property type="entry name" value="PX DOMAIN-CONTAINING PROTEIN YPR097W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12825">
        <property type="entry name" value="DUF3818"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12828">
        <property type="entry name" value="PXB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PX" evidence="2">
        <location>
            <begin position="255"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="310"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="324"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR24241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="991" mass="113425" checksum="F7762A93B120C4FF" modified="2009-07-28" version="1">MTLDLTPAEEHYLKRELLKQELVREIEGLDDPKALRRFGYPFSAEDPRSAHSKKKLIRRLKRSKASETESDSESNEDQGSEFPLLSHFLQHFVMKLPLLAKDLVNKEDFWQEKVQLFFEHFMEQQFSSSFDREELTKRKRLATKLSKMVLLMYNSGIGCKKEVEYFEQDKFELEGSKRPLKQQKLDQFVMPSKESLRYLLTEEPYYINGWDFNVMAAVSHKTLNPEPPAKAKASSSSGSKWMKTPAFLSPSSLTSPSKFFSRLTMTDSSSEKGEYIFVTRIRQEGAEDAWFINKKYSDFKRLVSDLNKEFPGKELPKLPHQSKVGVSSTTSFEAPSDKSNTSQPSTPREKIVSTFSEEASSLEAMPDEALIRGGDDDDDFQDAAETVTKHLPREKLRTSLRKFLRVLVEDNEVAQGVSLKKFVRGSKLEYAALPPPVLQDMKSREAVDVVNLENQIVFQKLAFEQTLQLQNAMKEFKTSVLEDEDYLLKLFQEIKVKDCVEQLSPALKGFMGWCRINLSATIYQTFLGNDGGYELFTQVKRLHRLLPYTVMIQILKITNPMAIMKGMMDLFMARPFGGNSLLQNMFSSVLSDDLKSQLKLAKELEESAARESNFGSEVSKVLASAIFDNEDGKLLDMQAVHDEAVEMNMPLAIVLAMKCNEKGYLSHDALGELIESYSLWKTQENTTSEALFKTDSSTSIEDVPGLYFSHIKSLLQIYIRERDKRLMKQIWQDPELSQLLKSIVALFYEPMVRIFRVSRMDVAFRNFERFMNDLVSLLDGVINGRNSFSTSFNVVDAINKLVEKHENSLYQFIHEICMHDTEKTFEGMITYLTSIVKFLQKSKYGQTDRLDLSELANGGVAQGLDTALLKKQLAELVSSKEESRKLYQKLVDAKTSSPDKKSENLKEAMERKWKESNEAALPETVTELGLQDGDLVDLDLDVGDYAYLDEEEQEALQQEHREILDKKVDLSEVSKMVDVVFKDKLKEILKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLB7</accession>
    <name>C5DLB7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">glycine--tRNA ligase</fullName>
            <ecNumber evidence="4">6.1.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Diadenosine tetraphosphate synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">KLTH0F11660g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the ATP-dependent ligation of glycine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (Gly-AMP). Also produces diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs. Thereby, may play a special role in Ap4A homeostasis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>2 ATP + H(+) = P(1),P(4)-bis(5'-adenosyl) tetraphosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:34935"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58141"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.14" evidence="4"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554705.1">
        <property type="nucleotide sequence ID" value="XM_002554659.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLB7"/>
    <dbReference type="FunCoup" id="C5DLB7">
        <property type="interactions" value="1180"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLB7"/>
    <dbReference type="GeneID" id="8292918"/>
    <dbReference type="KEGG" id="lth:KLTH0F11660g"/>
    <dbReference type="eggNOG" id="KOG2298">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015515_1_2_1"/>
    <dbReference type="InParanoid" id="C5DLB7"/>
    <dbReference type="OMA" id="MEMQYFV"/>
    <dbReference type="OrthoDB" id="57698at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004820">
        <property type="term" value="F:glycine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070150">
        <property type="term" value="P:mitochondrial glycyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00774">
        <property type="entry name" value="GlyRS-like_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00858">
        <property type="entry name" value="GlyRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.720.200:FF:000001">
        <property type="entry name" value="Glycine--tRNA ligase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.800:FF:000004">
        <property type="entry name" value="Glycine--tRNA ligase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000158">
        <property type="entry name" value="Glycyl-tRNA synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.230:FF:000002">
        <property type="entry name" value="Glycyl-tRNA synthetase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000010">
        <property type="entry name" value="Glycyl-tRNA synthetase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.720.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027031">
        <property type="entry name" value="Gly-tRNA_synthase/POLG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033731">
        <property type="entry name" value="GlyRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002315">
        <property type="entry name" value="tRNA-synt_gly"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00389">
        <property type="entry name" value="glyS_dimeric"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003211">
        <property type="entry name" value="PRK04173.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10745:SF0">
        <property type="entry name" value="GLYCINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10745">
        <property type="entry name" value="GLYCYL-TRNA SYNTHETASE/DNA POLYMERASE SUBUNIT GAMMA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01043">
        <property type="entry name" value="TRNASYNTHGLY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="15">
        <location>
            <begin position="181"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00051967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00058014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAR24268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="661" mass="75003" checksum="F902F82CC5FDE313" modified="2009-07-28" version="1">MSVEEVAQAKNAVEFSRENLESVLKRRFFFAPSFELYGGVSGLYDYGPPGCAFQANVIDVWRKHFILEEDMLEVDCSMLTPYEVLKTSGHVDKFSDWMCRDLKTGEIFRADHLVEEVLESRLKGDQEARGLTKDANANAQEDADKKKRKKKVKEIKAVKLDDNVVKEYEEILAKIDGYSGQELGELMIKYNIGNPVTGEALEPPMAFNLMFETAIGPSGQLKGYLRPETAQGQFLNFNKLLEFNNHKTPFASASIGKSFRNEISPRSGLLRVREFLMAEIEHFVDPLNKTHPRFDEVKNIKLRFLPREVQQSGSTEPIETTIEDAVATKMVDNQTLGYFIARIYSFLMAIGVDSNKLRFRQHMANEMAHYAADCWDAELKTSYGWIECVGCADRSAYDLTVHANKTKEKLVVRQKLEEPVQVTKWEIELTKKLFGPKFRKDAPKVESFLLNLSQEELEAKAKELKAGGKIVFEVEGMDSQIELDDKFVSIEQVTRTEHVREFVPNVIEPSFGIGRIIYSVFEHVFWSRPEDSARAVLSFPPLVAPTKVLLVPLSNHPDLSSVAHQVSKVFRKEKIPFKVDDSGVSIGKRYSRNDELGTPFGVTIDFESAKDGTVTLRERDSTKQVRGTVEEVVKAIRDITYNGVTWEEGTKSLKPFETQSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLT5</accession>
    <name>C5DLT5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Derlin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0G03322g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">May be involved in the degradation of misfolded endoplasmic reticulum (ER) luminal proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Endoplasmic reticulum membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the derlin family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555183.1">
        <property type="nucleotide sequence ID" value="XM_002555137.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLT5"/>
    <dbReference type="FunCoup" id="C5DLT5">
        <property type="interactions" value="523"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLT5"/>
    <dbReference type="GeneID" id="8293447"/>
    <dbReference type="KEGG" id="lth:KLTH0G03322g"/>
    <dbReference type="eggNOG" id="KOG0858">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059047_0_0_1"/>
    <dbReference type="InParanoid" id="C5DLT5"/>
    <dbReference type="OMA" id="TYGVACF"/>
    <dbReference type="OrthoDB" id="19102at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007599">
        <property type="entry name" value="DER1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11009">
        <property type="entry name" value="DER1-LIKE PROTEIN, DERLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04511">
        <property type="entry name" value="DER1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="64"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="172"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="215"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR24746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="349" mass="39256" checksum="A1A167B4443CA031" modified="2009-07-28" version="1">MISVVRLYLETTTSNRVLSNTSISSLLIARENHQTLQMAVPKKQSNSNELAQVVSQIPPVTRTLLAGVVAMTIVSSMGIVPREWLLFFLYPTFMKLQLWRMYTSCFLLPMDKMAAVFWMYNLYSYSSHLENAHFYSKNNVDYLFLLWSLIGAIVVSVTALRLDLSYNLTNSFMGALACIWSIKNWNVTFMFYGLFPLKGKYDPLFQLFLAFVFENHPGGFMLTLIGYCVGYLYVCLDTRTLGPLYGYIAGKTSSYGLADCGHFRAPWWFSAVCEFFGKSKHKVNVIIDKTTSYQGQGHRLGSEKAAAADAARVAAARAERFKTTTVQTNHKTTASGFFPGKGQRVGSKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DM15</accession>
    <name>C5DM15_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Arginine N-methyltransferase 2</fullName>
            <ecNumber evidence="6">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0G05170g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">S-adenosyl-L-methionine-dependent protein-arginine N-methyltransferase that methylates the delta-nitrogen atom of arginine residues to form N5-methylarginine (type IV) in target proteins. Monomethylates ribosomal protein L12.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the class I-like SAM-binding methyltransferase superfamily. RMT2 methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="6"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555263.1">
        <property type="nucleotide sequence ID" value="XM_002555217.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DM15"/>
    <dbReference type="FunCoup" id="C5DM15">
        <property type="interactions" value="428"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DM15"/>
    <dbReference type="GeneID" id="8293529"/>
    <dbReference type="KEGG" id="lth:KLTH0G05170g"/>
    <dbReference type="eggNOG" id="KOG1709">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033831_0_0_1"/>
    <dbReference type="InParanoid" id="C5DM15"/>
    <dbReference type="OMA" id="YWVVDNY"/>
    <dbReference type="OrthoDB" id="19014at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019702">
        <property type="term" value="F:protein arginine N5-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000310">
        <property type="entry name" value="Arginine N-methyltransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017408">
        <property type="entry name" value="Arginine_N-MeTrfase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051038">
        <property type="entry name" value="RMT2/GAMT_Mtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026480">
        <property type="entry name" value="RMT2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32379">
        <property type="entry name" value="GUANIDINOACETATE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32379:SF1">
        <property type="entry name" value="GUANIDINOACETATE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038148">
        <property type="entry name" value="Arginine_N-mtfrase-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51559">
        <property type="entry name" value="SAM_RMT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RMT2" evidence="8">
        <location>
            <begin position="181"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="142"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="142"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="172"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR038148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR24826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="403" mass="45410" checksum="C6D115BB12679237" modified="2009-07-28" version="1">MASLHELLTLKTLPVDGTYVAELTRLLKSGIPATYTIEQVAAHEAGQDEADDTGSNTTPLHVLLRSLPDELSEEECQVVLRLMDVLFEFGAGWNFLDYENKSPGDLLVEQGYSCGHPLYERVVEAGVCAELLLRKLNDDIEFIEGPEDEDEEDEETEEPAAAETTADATAEADEKHGAPARDTAEHQDAYLKADLEYTDGSLVTKENKDGVMMDWETDIMRLARDSMFKHCGSGAVVLNIGFGMGIIDTFIQERGPAKHYICEAHPDVLVHMRSEGWYDKPNVVVLEGRWQDSLSALLDEGTVFFDAIYYDTFSEHYSDMLELYDTIVGLIKPEGLFSFFNGLGADRQVCYDVYKRIVELDVRNYGMDCKYTIVPLPQNPDWAGVRRSYFACDRYYHPEIRFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>C5DM19</accession>
    <name>C5DM19_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">t-SNARE affecting a late Golgi compartment protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0G05258g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the syntaxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555267.1">
        <property type="nucleotide sequence ID" value="XM_002555221.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DM19"/>
    <dbReference type="FunCoup" id="C5DM19">
        <property type="interactions" value="299"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DM19"/>
    <dbReference type="GeneID" id="8293533"/>
    <dbReference type="KEGG" id="lth:KLTH0G05258g"/>
    <dbReference type="eggNOG" id="KOG3202">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061883_0_2_1"/>
    <dbReference type="InParanoid" id="C5DM19"/>
    <dbReference type="OMA" id="NDCCIGL"/>
    <dbReference type="OrthoDB" id="546861at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15851">
        <property type="entry name" value="SNARE_Syntaxin6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000006">
        <property type="entry name" value="Syntaxin 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006012">
        <property type="entry name" value="Syntaxin/epimorphin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF224">
        <property type="entry name" value="HL02043P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00914">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="188"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="11">
        <location>
            <begin position="115"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="84"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="85"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00073343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR24830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="207" mass="23781" checksum="7D48DEB6170274B3" modified="2009-07-28" version="1">MDDPFEQVLNDTREQASRLAQYLDAGHELDQEVRDILEDLEETVRELDSSIMVMRREGFVDNATILARENRMQDLKRELARLKTHMTSSSRSAQGDGSPETSEDPSSPRNNLLQEQMMEEQDQHLDTIHQTMQNLHLQASTMGQELEDQGMLLEEMDGGVDNVMSKLSRGRRQLEWVYEKNKEKYNDCCIGLLIVALIVLLVLAFVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>C5DM31</accession>
    <name>C5DM31_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0G05566p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0G05566g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555279.1">
        <property type="nucleotide sequence ID" value="XM_002555233.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DM31"/>
    <dbReference type="FunCoup" id="C5DM31">
        <property type="interactions" value="66"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DM31"/>
    <dbReference type="GeneID" id="8293546"/>
    <dbReference type="KEGG" id="lth:KLTH0G05566g"/>
    <dbReference type="HOGENOM" id="CLU_2483758_0_0_1"/>
    <dbReference type="InParanoid" id="C5DM31"/>
    <dbReference type="OMA" id="AIKPAQG"/>
    <dbReference type="OrthoDB" id="2094445at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021278">
        <property type="entry name" value="ATP19"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11022">
        <property type="entry name" value="ATP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR24842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="87" mass="9110" checksum="490F638EB0C45053" modified="2009-07-28" version="1">MLVPKRILLASAIKPAQGDEVERCLTIQPHVLAIATIASTVGVAAYSMSGSKAEAAPQPASAMSTQESGGLDVEKLIDNYLKQSEKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>C5DM71</accession>
    <name>C5DM71_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">eIF-2-alpha kinase GCN2</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="ordered locus">KLTH0G06468g</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555319.1">
        <property type="nucleotide sequence ID" value="XM_002555273.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DM71">
        <property type="interactions" value="937"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DM71"/>
    <dbReference type="GeneID" id="8293587"/>
    <dbReference type="KEGG" id="lth:KLTH0G06468g"/>
    <dbReference type="eggNOG" id="KOG1035">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001222_2_0_1"/>
    <dbReference type="InParanoid" id="C5DM71"/>
    <dbReference type="OMA" id="FEDIAWD"/>
    <dbReference type="OrthoDB" id="341578at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004694">
        <property type="term" value="F:eukaryotic translation initiation factor 2alpha kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140469">
        <property type="term" value="P:GCN2-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990625">
        <property type="term" value="P:negative regulation of cytoplasmic translational initiation in response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009893">
        <property type="term" value="P:positive regulation of metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14012">
        <property type="entry name" value="PK_eIF2AK_GCN2_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23823">
        <property type="entry name" value="RWD_GCN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14046">
        <property type="entry name" value="STKc_EIF2AK4_GCN2_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000856">
        <property type="entry name" value="eIF-2-alpha kinase GCN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001061">
        <property type="entry name" value="eIF-2-alpha kinase GCN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000050">
        <property type="entry name" value="eIF-2-alpha kinase GCN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000379">
        <property type="entry name" value="eIF-2-alpha kinase GCN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050339">
        <property type="entry name" value="CC_SR_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016255">
        <property type="entry name" value="Gcn2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041715">
        <property type="entry name" value="HisRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024435">
        <property type="entry name" value="HisRS-related_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006575">
        <property type="entry name" value="RWD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042:SF136">
        <property type="entry name" value="EIF-2-ALPHA KINASE GCN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12745">
        <property type="entry name" value="HGTP_anticodon2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05773">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13393">
        <property type="entry name" value="tRNA-synt_His"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000660">
        <property type="entry name" value="Ser/Thr_PK_GCN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00591">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50908">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0010">Activator</keyword>
    <keyword evidence="10 20 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="22" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 20 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="13" id="KW-0346">Stress response</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="5" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="RWD" evidence="25">
        <location>
            <begin position="17"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="218"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="599"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="550"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="670"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="726"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1557"/>
            <end position="1582"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="22">
        <location>
            <begin position="136"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="23">
        <location>
            <begin position="676"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="23">
        <location>
            <begin position="731"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="763"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="793"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="19">
        <location>
            <position position="884"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <begin position="605"/>
            <end position="613"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="628"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="629"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00037982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00073598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR000660-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR000660-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EMBL" id="CAR24882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1710" mass="193475" checksum="507490F885F89460" modified="2009-07-28" version="1">MAPGSFTLDQNYELQKNELEAIKCIYMDDFTDKTVQKSSWDKHPLIQFEISLRSTDQEPAESSLTLDVTLPAKYPQEPPVISFKNIQNVLGSYLASIKAEINEIHKRSKGREEIIFEITSLVQEKLDEAQSSANTQSLEDERLQRIEDEKKKLEEEESKRKEDVKVERLKEQKLIDEIVRKEMEKRQDDDLSFKPDSTIDFNPPIDWITSGEAYVFPKMIRAKLPNNSFYKFKAVVNPQQIKLSNDLLGFSKQFLVKPYIPPESPLANTLASSDIMDNFFYLLTIVTLDNPYFNTNNGKKDISNLEKELDSLLHVRHDNINRLYAFAVERTGRSNSSYAWKIRILNEYSPLSLVGDVVDSVGYVNIATARGWVLRLIEGLESLHKQGVVHKSVNLQTVTLAKDADFGTTIPKLLHSGYGYTILNLISAHPSKTGPKIEMIEQPWVAPELIRFNNSKPQRKTDVWELGVLFVQTINGADTVVKFPTPQEFLSNVPMDESLFDFLQKLLEPDLKKRFGPLELLPMKFLRTNLDPTINKNLFPDSTSISAMNSSAALASGPEPRSSMSLSTTSGDIRRRSFNVSSRYSSTNPSSNSRYATDFEEIAVLGKGAFGQVVKARNALDSRYYAVKKIRHSEEKLSSILSEVMLLASLNHQYVVRYYAAWLEEDTQEQAIASSDSEETDSIETSEIEDYTESSTFNSTGQAFNNGWDFISNSLQASNYPEIVFANSSSENEEEKEKEYESEDFESSEGGSCARTSADFSLDEQKRSDGKGHQGQQTRRKGVFSAGACTGTHDMRQGAKHDSGKDDKVAPRYAGKETQAERRFSRRGGKSTLFIQMEYCENRTLFDLIHNENLSAQREEYWKLFREILDALSYIHSQGIIHRDLKPMNIFIDESRNIKIGDFGLAKNVNKPVDLLRMDSHMSAASAEDLTSAVGTALYVAVEVLSGSGNYNEKIDMYSLGIIFFEMVYSFNTGMERVNDIKKLRSSSIEFPSDFDDTKLAVEKKIIKQLLDHDPKKRPDAKTLLRSGLLPVKHQDDVIKEALRNLADPSSPWQQQVKESLFAQPYSVTNDILFDNHKVFSTPFNQLLKSQMMEEIVKIFKRHGGIENNEPPMIFPKAPVYSTQNVYEVLDKGGSVLQLQYDLTYPMARYISKGPNCVSKQYRVQYVYRPPQHAQPSTEPRRFVEVDFDIITPPNSETPLYDAESIKVIDEIIIQLPIFGKTNTIFAVNHADILESVFNFCSIDKAQRSLVSHMLSQVGFAKTFKDVKNELKSVLNISSTSLNDLELFDFRLDFESARKRLQKVMMDSPFLAKVEESLQYISKVINFLKPLKVSRNIIICPISNYNWGFYKGNIMFQAIYDDDKSRSLIAAGGRYDSLISLIARPSGGKINNIQRAVGFNLAWETILAVAQNYFKLATGKSTKKRSSLMKDVPLEWKPMRCEVLVSSFSKTILNTIGVETLNQLWKAGISADFVHSCYTVDDVVSAAQRDGVNWIILIKQQKYSAPSNKRKYKPLKVKKLGSELDVDLDFDEFLAIYQQEAESKFISSDFPQILESNPVSPDDKRWEDFTSTEESQDGASDTSAIGKTAQKVVYIPNMATRAKKSNKKDKWVYEDSARNASRAIISSLSTAPIFAVDAVRDETLEIISVTSLAQKDEWLRKIFGVGNNSAPRSFATSIYNNLAKEASKGGKWAIVHCDKTGKSCVVDLQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DM91</accession>
    <name>C5DM91_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0G06930p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0G06930g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555339.1">
        <property type="nucleotide sequence ID" value="XM_002555293.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DM91"/>
    <dbReference type="FunCoup" id="C5DM91">
        <property type="interactions" value="150"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DM91"/>
    <dbReference type="GeneID" id="8293608"/>
    <dbReference type="KEGG" id="lth:KLTH0G06930g"/>
    <dbReference type="eggNOG" id="KOG1347">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012893_1_2_1"/>
    <dbReference type="InParanoid" id="C5DM91"/>
    <dbReference type="OMA" id="WFFVWKL"/>
    <dbReference type="OrthoDB" id="2126698at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13132">
        <property type="entry name" value="MATE_eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045069">
        <property type="entry name" value="MATE_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="160"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="199"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="229"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="274"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="302"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="331"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="388"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="464"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="497"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="533"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="564"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="71"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="80"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR24902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="605" mass="66491" checksum="63C02320EAFCE138" modified="2009-07-28" version="1">MAPPSKQFTHTTGERRSSVIHSSGIIKGGIFAPVDYIPQEENAIGDVDSDDGENRYESLLDSNNAYTLQGEGLSSYGATSDSNNGASKRSSSRNSYSRDLLEEERDLLLDNKLISDDGPLNSVPEEEEEQIRQTWEDALNSGKIISTTYRRECQVLMKNAFPLIFTFVLQNSLSLASVFTTSHLGIKELGGITLGSMTANITGFAAIQGLCTCLDTLCSQAYGAKNYHLVGILLQRCALITIIFFTPILCIWWLWSEAILAALIPERELCRLAANYLKVVALGVPGFVFFECGKRFLQCQGIFHASTIVLLFCAPFNAVMNYMLVLDKRFGIGYLGAPISVAVNYWLMALGLLLYTMFTNHKINPRKCWSGTIKPNQIFKNWRKMFSLALPGIVMVEAEFLGFEILTIFASHLGTRELGAQSIVSTIASLAYQVPFSISISTSTRVANFIGASLYQNCIATCKVSLLLSAVASCFNMTIIYAYRVPLANAFSKEPDVVNLVVSVLPLLAFMQIFDAFNACTAGCLRGQGQQKIGGYINVLAFYCVGIPMSYFLTFNCGLGMAGLWYGITSALVFMSIFQSYAVFSCNWKEAIQAAKSRNSEGSRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMC4</accession>
    <name>C5DMC4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0G07700p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0G07700g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAD2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555372.1">
        <property type="nucleotide sequence ID" value="XM_002555326.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMC4"/>
    <dbReference type="FunCoup" id="C5DMC4">
        <property type="interactions" value="1208"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMC4"/>
    <dbReference type="GeneID" id="8293643"/>
    <dbReference type="KEGG" id="lth:KLTH0G07700g"/>
    <dbReference type="eggNOG" id="KOG3285">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072097_0_0_1"/>
    <dbReference type="InParanoid" id="C5DMC4"/>
    <dbReference type="OMA" id="WQFDVEI"/>
    <dbReference type="OrthoDB" id="1806at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033597">
        <property type="term" value="C:mitotic checkpoint complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.900.10:FF:000002">
        <property type="entry name" value="Mitotic spindle assembly checkpoint protein MAD2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.900.10">
        <property type="entry name" value="HORMA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003511">
        <property type="entry name" value="HORMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036570">
        <property type="entry name" value="HORMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045091">
        <property type="entry name" value="Mad2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11842">
        <property type="entry name" value="MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11842:SF11">
        <property type="entry name" value="MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02301">
        <property type="entry name" value="HORMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56019">
        <property type="entry name" value="The spindle assembly checkpoint protein mad2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50815">
        <property type="entry name" value="HORMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0498">Mitosis</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HORMA" evidence="7">
        <location>
            <begin position="8"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR24935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="199" mass="23217" checksum="375E250ED0FFF84D" modified="2009-07-28" version="1">MASSLSLKGSTRIVTEFFEYSINSILYQRAVYPPEDFVTVRKYDLNLLKTHDDELKSYIRQILLQVHRWLLGGKCNKLVLCIVDKSTCDIVERWEFDVQHNGQESYTEQTKEVENAETQKQIRALMRQITASVTFLPELEENDNYTFNVLAYTDANAKVPLEWADSDSMDIPDGERVEFRSFSTNNHKIRTQVSYKMNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMI5</accession>
    <name>C5DMI5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">L8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">RP24</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">YL11</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0G09218g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555433.1">
        <property type="nucleotide sequence ID" value="XM_002555387.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMI5"/>
    <dbReference type="FunCoup" id="C5DMI5">
        <property type="interactions" value="1001"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMI5"/>
    <dbReference type="GeneID" id="8293710"/>
    <dbReference type="KEGG" id="lth:KLTH0G09218g"/>
    <dbReference type="eggNOG" id="KOG3255">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065464_0_0_1"/>
    <dbReference type="InParanoid" id="C5DMI5"/>
    <dbReference type="OMA" id="YAHFPMK"/>
    <dbReference type="OrthoDB" id="10252633at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.930.12:FF:000003">
        <property type="entry name" value="60S ribosomal protein L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.930.12:FF:000004">
        <property type="entry name" value="60S ribosomal protein L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.930.12">
        <property type="entry name" value="Ribosomal protein L6, alpha-beta domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000702">
        <property type="entry name" value="Ribosomal_uL6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036789">
        <property type="entry name" value="Ribosomal_uL6-like_a/b-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020040">
        <property type="entry name" value="Ribosomal_uL6_a/b-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002359">
        <property type="entry name" value="Ribosomal_uL6_CS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655:SF16">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655">
        <property type="entry name" value="60S/50S RIBOSOMAL PROTEIN L6/L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00347">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002162">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56053">
        <property type="entry name" value="Ribosomal protein L6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00700">
        <property type="entry name" value="RIBOSOMAL_L6_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL6 alpha-beta" evidence="7">
        <location>
            <begin position="12"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL6 alpha-beta" evidence="7">
        <location>
            <begin position="97"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00075840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00080918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00082770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR24996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="191" mass="21507" checksum="B651D35F2E4B48F3" modified="2009-07-28" version="1">MKYIQTDQTLDIPEGVTVNIKSRVIKVTGPRGALTKNLKHIDVTFAKVSNKQVKITVHNGDRKHVAALRTVKSLVANMITGVTKGFKYKMRYVYAHFPINVNVVEKDGAKFVEIRNFLGDKQVRMVPVREGVSIDFSTNQKDEIVLSGNSIENVSQNAADIQQICRVRNKDIRKFLDGIYVSQKGFVEEEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMY9</accession>
    <name>C5DMY9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0G12760p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0G12760g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555587.1">
        <property type="nucleotide sequence ID" value="XM_002555541.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMY9"/>
    <dbReference type="FunCoup" id="C5DMY9">
        <property type="interactions" value="78"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMY9"/>
    <dbReference type="GeneID" id="8293870"/>
    <dbReference type="KEGG" id="lth:KLTH0G12760g"/>
    <dbReference type="eggNOG" id="ENOG502S42T">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105987_1_0_1"/>
    <dbReference type="InParanoid" id="C5DMY9"/>
    <dbReference type="OMA" id="SCEDHAC"/>
    <dbReference type="OrthoDB" id="3998251at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025928">
        <property type="entry name" value="Flocculin_t3_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13928">
        <property type="entry name" value="Flocculin_t3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950436" evidence="6">
        <location>
            <begin position="19"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR25150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="121" mass="11792" checksum="4600DBDC222B3DCC" modified="2009-07-28" version="1">MQFSTAAAVATVTAVVAATNTTTVHSDSTTLVTITSCEEHACSESVSPALVSTATVTVNDVVTKYTTWCPLPTTAAPSSSNGTKTEAPASSTASVTSYTGAAAKALPAAGALVAGAAALLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMZ9</accession>
    <name>C5DMZ9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Fimbrin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0G13024g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555597.1">
        <property type="nucleotide sequence ID" value="XM_002555551.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMZ9"/>
    <dbReference type="FunCoup" id="C5DMZ9">
        <property type="interactions" value="649"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMZ9"/>
    <dbReference type="GeneID" id="8293880"/>
    <dbReference type="KEGG" id="lth:KLTH0G13024g"/>
    <dbReference type="eggNOG" id="KOG0046">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015284_3_0_1"/>
    <dbReference type="InParanoid" id="C5DMZ9"/>
    <dbReference type="OMA" id="WQLMRKN"/>
    <dbReference type="OrthoDB" id="431378at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032432">
        <property type="term" value="C:actin filament bundle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051017">
        <property type="term" value="P:actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051639">
        <property type="term" value="P:actin filament network formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110009">
        <property type="term" value="P:formin-nucleated actin cable organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd21294">
        <property type="entry name" value="CH_FIMB_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21297">
        <property type="entry name" value="CH_FIMB_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21300">
        <property type="entry name" value="CH_FIMB_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21303">
        <property type="entry name" value="CH_FIMB_rpt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000042">
        <property type="entry name" value="Fimbrin, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000010">
        <property type="entry name" value="Plastin-3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000016">
        <property type="entry name" value="Probable fimbrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000027">
        <property type="entry name" value="Probable fimbrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001589">
        <property type="entry name" value="Actinin_actin-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039959">
        <property type="entry name" value="Fimbrin/Plastin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19961:SF18">
        <property type="entry name" value="FI19014P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19961">
        <property type="entry name" value="FIMBRIN/PLASTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00020">
        <property type="entry name" value="ACTININ_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="51"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="7">
        <location>
            <begin position="140"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="7">
        <location>
            <begin position="288"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="7">
        <location>
            <begin position="412"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="7">
        <location>
            <begin position="535"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="84"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="95"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00073963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR25160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="643" mass="71310" checksum="370744806DAAADED" modified="2009-07-28" version="1">MNIIKLQRKFPILTQEDIFSTIEKFRDIDLEDKGWIEKGQLLAAVSKSGDTSYDEARETLKQVNVDASGRVELDDYVELVAKLKDAKAGPPPPTSFNTGSGPSNAPTPASHAGLKHSGSGAQARIIVGGSTTGTTHTINEEERTEFTKHINSVLAGDPDVGDKLPFPTDTFQLFDECRDGLVLSKLINDSVPDTIDTRVLNWPKNGKRLNNFTASENANIVINSAKAIGCVVVNVHSEDIIEGKEHLIMGMIWQIIRRGLLSKIDIKHHPELYRLLEDDETLEQFLRLPPEQILLRWFNYHLKQAGWSRRVANFSKDVADGENYTILLNQLAPELCTRAPLQTTDLLTRAEQVLQNAEKLDCRKYLTPSALVAGNPKLNLAFVAHLFNTHPGLQPIEESENIEIEEFDAEGEREARVFTLWLNSLDVDPPVVSLFEDLKDGLVLLQAYEKVMPGAVNWKVVNKRPSSGAEVSRFKALENNNYAVELGKTRGFSLVGIEGSDIVDGNKLLTLGLVWQLMRRNIVNTMKTLSGNGKELSDGEILKWAQEQVTKGGKTSHVRSFKDPALSNAHFLLDVLNGIAPGYVDYALVTPGVTDEDKYANARLAISIARKLGALIWLVPEDINEVRSRLILTFVASLMALKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DN46</accession>
    <name>C5DN46_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0G14080p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0G14080g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555644.1">
        <property type="nucleotide sequence ID" value="XM_002555598.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DN46"/>
    <dbReference type="FunCoup" id="C5DN46">
        <property type="interactions" value="111"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DN46"/>
    <dbReference type="GeneID" id="8293928"/>
    <dbReference type="KEGG" id="lth:KLTH0G14080g"/>
    <dbReference type="eggNOG" id="ENOG502QQID">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013813_0_0_1"/>
    <dbReference type="InParanoid" id="C5DN46"/>
    <dbReference type="OMA" id="SHQECLI"/>
    <dbReference type="OrthoDB" id="299997at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16448">
        <property type="entry name" value="RING-H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11070">
        <property type="entry name" value="Protein Ste5, Fus3-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038382">
        <property type="entry name" value="Ste5_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021106">
        <property type="entry name" value="Ste5_Fus3-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12194">
        <property type="entry name" value="Ste5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="168"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="23"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR25207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="940" mass="104564" checksum="DEE5E5120923DD83" modified="2009-07-28" version="1">MLSPDQTQEQLIFQLANIGGHNVPMRDDCSSSLTTPRSSRSRRWGQGLTKTPKPKNGFVMQECSQPGLGIGFPGLEPPWGSTATPTPRSHSLSVLEIPSRSTSIPRPESKFLKPPFALASSSSFEEWNTPVSGPLPYQLSAGVTPPRVRPFDHPSHSTSKKSYIHEPCSLCLEFISCRTLGEKVVPLECGHLVHEECLVAYFDSPTKFDINDMFPYCRKCDNDVRCLPSDLQLRDSCISRALVSGSPRFKRFQDIAPKERLVDKVSSDSISIPNRLLSMEDFRLNAGPSRSRSNKNVKDLSLRLKPNNNFRGSVVSGISSVISSVSDFSVSQPQDSNGGMSITEHKPPLAALRSYYTHLLMENFPNQLHGWKIDANFGLLRLVDRLMFSEDGKKYQDACCYLFTNALLIALVSPTFDPKISAGIRLHTYVTYHPLSDTSVDSLNSSVLKCTILSATPNAKLMHGSTLYLTEALDSASSQVIEKWISALLNKDILFGNSALSSTLPPPPIIEEETRRSLMVELPGASSLDLDVSEQIDDNEAIVRTSTVISNNQSRRTTITSLLSLKRGRPTLLALVLQLDPSRLKDAELIGLVNSLMALTFKIRDARLCLVDLEGGIVSQGLVREQLSVVKDLKSTVKDHNRARFNPADFKNEFIWENVDKIGIAVISNTLVESGKSCLFMDYNSFAGINRTRPHELKIHIGFLNVDYTDCISELVEVASAHDLLETLCYGFNLSFEEEDDNNDDDDEDFDNDVSYEYDVKLDRFDDLHTLKTPGSDHTKASFIIVDNDNSAGLLTNTLNDEMLETDEWISTRSPLLRENRTSCSIESPLTDNLEIKEVPGSSSPIGEDVSLNAVLAKLHLQDFDEKFKKRTTEKPEIRQKQLKRSSKFINKICIDELAQESSINEAQNTLLGWTAFLKGISKQLDDAMDDKNEAEATPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>C5DN56</accession>
    <name>C5DN56_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GTP:AMP phosphotransferase, mitochondrial</fullName>
            <ecNumber evidence="7">2.7.4.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Adenylate kinase 3</fullName>
            <shortName evidence="7">AK 3</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ADK2</name>
        <name evidence="10" type="ordered locus">KLTH0G14322g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in maintaining the homeostasis of cellular nucleotides by catalyzing the interconversion of nucleoside phosphates. Has GTP:AMP phosphotransferase and ITP:AMP phosphotransferase activities.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a ribonucleoside 5'-triphosphate + AMP = a ribonucleoside 5'-diphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:13749"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.10"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon GTP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent GTP hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the adenylate kinase family. AK3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.10" evidence="7"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555654.1">
        <property type="nucleotide sequence ID" value="XM_002555608.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DN56"/>
    <dbReference type="FunCoup" id="C5DN56">
        <property type="interactions" value="373"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DN56"/>
    <dbReference type="GeneID" id="8293939"/>
    <dbReference type="KEGG" id="lth:KLTH0G14322g"/>
    <dbReference type="eggNOG" id="KOG3078">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032354_1_1_1"/>
    <dbReference type="InParanoid" id="C5DN56"/>
    <dbReference type="OMA" id="TIAHFST"/>
    <dbReference type="OrthoDB" id="439792at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:AMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046899">
        <property type="term" value="F:nucleoside triphosphate adenylate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006172">
        <property type="term" value="P:ADP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046033">
        <property type="term" value="P:AMP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046039">
        <property type="term" value="P:GTP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046041">
        <property type="term" value="P:ITP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01428">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000106">
        <property type="entry name" value="Adenylate kinase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00235">
        <property type="entry name" value="Adenylate_kinase_Adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03169">
        <property type="entry name" value="Adenylate_kinase_AK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006259">
        <property type="entry name" value="Adenyl_kin_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000850">
        <property type="entry name" value="Adenylat/UMP-CMP_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033690">
        <property type="entry name" value="Adenylat_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007862">
        <property type="entry name" value="Adenylate_kinase_lid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028586">
        <property type="entry name" value="AK3/Ak4_mitochondrial"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01351">
        <property type="entry name" value="adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23359">
        <property type="entry name" value="NUCLEOTIDE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00406">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05191">
        <property type="entry name" value="ADK_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00094">
        <property type="entry name" value="ADENYLTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00113">
        <property type="entry name" value="ADENYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 7 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Adenylate kinase active site lid" evidence="9">
        <location>
            <begin position="134"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="NMPbind" evidence="7">
        <location>
            <begin position="34"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="LID" evidence="7">
        <location>
            <begin position="133"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="13"/>
            <end position="18"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="61"/>
            <end position="63"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="92"/>
            <end position="95"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="143"/>
            <end position="144"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR25217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="225" mass="25090" checksum="76B385FF5F5D2117" modified="2009-07-28" version="1">MNPLRLLLLGAPGSGKGTQTSKLLNQFKHIKSVSSGDLLRRRIQQKTELGRIASEYISQGKLLPDSLMNDLVVGELSVRGWLSADSSFLLDGFPRTLEQARSLRVQLTPHNASLNLVVELDVPTDVILERIENRYVHVPSGRVYNLTYNPPKVPGKDDVTGEPLIKRPDDTSEVFGKRLKNYAETVEPLKQFYSELGILKTVSGPTSDIIFPQLLKLVVQKLHQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DN73</accession>
    <name>C5DN73_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0G14696p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0G14696g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555671.1">
        <property type="nucleotide sequence ID" value="XM_002555625.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DN73"/>
    <dbReference type="FunCoup" id="C5DN73">
        <property type="interactions" value="65"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DN73"/>
    <dbReference type="GeneID" id="8293956"/>
    <dbReference type="KEGG" id="lth:KLTH0G14696g"/>
    <dbReference type="eggNOG" id="KOG1659">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045277_9_1_1"/>
    <dbReference type="InParanoid" id="C5DN73"/>
    <dbReference type="OMA" id="IMKKTIM"/>
    <dbReference type="OrthoDB" id="653904at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017054">
        <property type="term" value="C:negative cofactor 2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001046">
        <property type="term" value="F:core promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22906">
        <property type="entry name" value="HFD_DRAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050568">
        <property type="entry name" value="Transcr_DNA_Rep_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10252:SF5">
        <property type="entry name" value="DR1-ASSOCIATED COREPRESSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10252">
        <property type="entry name" value="HISTONE-LIKE TRANSCRIPTION FACTOR CCAAT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription factor CBF/NF-Y/archaeal histone" evidence="3">
        <location>
            <begin position="16"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR25234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="106" mass="11926" checksum="9C7F475B98955F9E" modified="2009-07-28" version="1">MSETNGSEVFGNIKTHFPPAKIKKIMQTDEDIGKVSQATPVITGRSLEFFIAMLVNRSGHVAKEMGCRRISGEVMKKTIMTDEKFDFLRELICGDRAKEQTDEEEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNK9</accession>
    <name>C5DNK9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0G17886p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0G17886g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555807.1">
        <property type="nucleotide sequence ID" value="XM_002555761.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNK9"/>
    <dbReference type="FunCoup" id="C5DNK9">
        <property type="interactions" value="104"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNK9"/>
    <dbReference type="GeneID" id="8294101"/>
    <dbReference type="KEGG" id="lth:KLTH0G17886g"/>
    <dbReference type="eggNOG" id="KOG4462">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_386454_0_0_1"/>
    <dbReference type="InParanoid" id="C5DNK9"/>
    <dbReference type="OMA" id="PMRQSSN"/>
    <dbReference type="OrthoDB" id="2430277at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003124">
        <property type="entry name" value="WH2_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02205">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00246">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51082">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WH2" evidence="2">
        <location>
            <begin position="31"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="141"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="168"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="193"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="220"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="246"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="259"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="354"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="387"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="422"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="452"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="497"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="518"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="534"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="570"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="604"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="625"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="653"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="671"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="720"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="749"/>
            <end position="760"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR25370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="804" mass="78477" checksum="ED8720B8BECBAD34" modified="2009-07-28" version="1">MAGPPPPPPPMMPGAASGSSKPPAAPQMQMGRDALLGDIRKGARLKKAVTNDRSAPQVGGGVVSGNAPNPPTPASAPPVSQAQAMPPMPLPSGPQLGDILAGGIPKLRSTSGAPRGAPAPPVPGMSAPPTPAAPAPPAHPVPAGSAPGIPGSAPPIPGSAPPAPAAPQIPSSRPHMPSSRPTKRRSNIEGKAPPVPSAPPAPPAPPASAPPLPSSNAPGTPAPLLPQSSAPPAPPVPFAAAPPAPASSAPSVPKSSPSSAPPAPPAPSAPPVPGLPKSSAPPAPPAPPAPPAPPAPPAPPVPSAPALPKSGAPPAPPVPSAPALPKSGAPPAPPAPTLPKSSVPPAPPAPPALPASSAAPQRRAPSAASAPPAPMPAAGGLPFLAEIQKKRDDRFVIGENSKYTTKVEPTNDPEPVKAPTLDKTSQNAPSGQGMSFLDEMNSKLNAQHTKPPASPSIPPPPPTSGAPPVPRAPPAAPPPATKAPPLPSFSAPSTNAAPPPPPPPPVSSLPPPPPPIPTSSSPQAPALPKSSAPGSPPPPPPPPALGSGSSTNHQNAPKPSLGGGLPFLAEIEKKRDDSHVIEGVSSNSGPGKAENRTPSAPSVPAAPAPPVPSLPSFGAPPVPSPAKMASSGSSSSATMPAAPPLPSMGSPVLPTPPAPPAPPMASSDPHSAPPPPPMAPFSPASTATENGGSSKLPPPPPPPSSTATTSHKQRLFSNGDDAQSSHTLNPHTNQPEIKTSNLTISGSATTTGVKLGGTKVTVDDSRFSWGNAGQLPKPRAFSNKTKLYPSGRGSSVPLDLALYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNN7</accession>
    <name>C5DNN7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0G18612g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555835.1">
        <property type="nucleotide sequence ID" value="XM_002555789.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DNN7">
        <property type="interactions" value="302"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNN7"/>
    <dbReference type="GeneID" id="8294130"/>
    <dbReference type="KEGG" id="lth:KLTH0G18612g"/>
    <dbReference type="eggNOG" id="KOG0605">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000709_4_1_1"/>
    <dbReference type="InParanoid" id="C5DNN7"/>
    <dbReference type="OMA" id="HNRRFVG"/>
    <dbReference type="OrthoDB" id="162894at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901992">
        <property type="term" value="P:positive regulation of mitotic cell cycle phase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05611">
        <property type="entry name" value="STKc_Rim15_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000340">
        <property type="entry name" value="Serine threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001008">
        <property type="entry name" value="Serine/threonine-protein kinase cek1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF1">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE GREATWALL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PAS" evidence="14">
        <location>
            <begin position="43"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="679"/>
            <end position="1067"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="13">
        <location>
            <begin position="1390"/>
            <end position="1504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="517"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="881"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1105"/>
            <end position="1169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1222"/>
            <end position="1349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="18"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="529"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="540"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="552"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="905"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="932"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1109"/>
            <end position="1132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1133"/>
            <end position="1143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1156"/>
            <end position="1166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="1223"/>
            <end position="1232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1233"/>
            <end position="1246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1266"/>
            <end position="1277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR25398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1524" mass="168645" checksum="F3913C58244DE464" modified="2009-07-28" version="1">MMDLGSLSIGEGHEQRSARSTSPDSANSQVTSLGSRSGSTLSYDEYLKLATDTSASILLELEMNGIIRYVSPIWHDIVGTDTSDVVGKAISDILVGSDQDRNVFQRAVDLMLREDCSYRVRFLVESGNQNQSENVIEADSESTDIAARDEKGIIELEAQGIVISESSKNLPTHSMWIVKPFCEMGALDNLPLELVKRLGFGATIFSQYLTQVESSMVTDEADLPTPKNELCRVCESPVPAWWLETHSESCIVEHKIESMVQLLHDKLVERKLFLEEMFASLEANDGRITNYKGLPLPKPEEGILATSEGNSIPHGSQSPLDIFKKSSNARKNSSSIFQSLSFPFKELAGLLELCDGAINTNTSELRGTSSANPLENESINLFYEFSPATQRNIEQVVKWNSQSDSKNPAVNLLKSDTMFLASQKIEYLFRLDNAMRYSLKIKNEIDNYVLQIIRERIERNRLNSISDTEVFRPMTKSPVCVTVNGSENVPGGKIASPQPQKPQGEIFSDAYVGTDEIPRDTLGHTARNSSAMSDSCSNSRPLSRSVTPKQTLAEHDKTHEETRQEDAEMFKSATGTPKLVLPEPNVHPKLSNIHEFTPRRDSLASSAGFNTPLSSLQRNFVSRPLNQSFDRSPVTSPYAASSDYLTPETHTALVPKQPLSPLLLATNQAKPSTPSIKDYDIIKPISKGAYGSVFLAKRKITGEYFAIKVLKKSDMIAKNQVTNVKSERAIMMVQSNKPYVARLFATFQNRGNLFLVMEYLSGGDLGTLIKMMGSLPDKWAKQYISEVIYGVADMHESGIIHHDLKPDNLLIDRRGHLKLTDFGLSRMGLVTRHTGVNQKGRLLTNSRKGSATSEEINLGGSFKWQLKSDSPSLANAFDNVGSKAERSNSNSSSQSMVDAPPLHKSGSQLSFSMTDISRDNTPPPCSLHRHTNSSVSENWENSPTPDYALYNPDDSRTEKKFFGTPDYLAPETIKGTGETDCSDWWSVGCMLFEFFFGYPPFHAPTVEEVFSNILAGKIDWPEFPDAETEKEYIPPEAKDLVEKLLVVDPNKRLGANGAQEIFDHPYFQDVNFVQLYEEDGSFVPEVAHPESTDYFDLRGAQLEDFSESDQEHPRANDLIEHSSERAPKRTNSERSSQSSTPVQKLTIGSVLEPPQESGSNHSSPTTKYIPLAIPPHLRERRVSKLNEVQTEFGSFNFRNLPALDKANKDAINRLKSEHLAEHAHHHLHHHRGLSGSSSSSDTSSKLRYAKPSQGAQPPGTPALSLKASTSPGSSKNHSPCRRDSVDNAMVSRRSAPSAVEVSPNSLSPNHDEGDSPSPAFRFKSPLSPQAASSRARLHSRTSSMRSSLGDFAVDEGERLKALSRANSTRGRRRSGRESSSGISDIAYNMDVLVCEPIPIHRFKLTRDLESLGCSVVSVSMGDEMVRRATSDVKFDLIFTALKLPKLGAIDIVKLLRNTNSVNSTTSIIAVTAYFQEAQQACAFDEVLERPVSVQQLRSLLLKYSLKKSQESEENSISDAEHEVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNQ2</accession>
    <name>C5DNQ2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Elongation factor G, mitochondrial</fullName>
            <shortName evidence="10">EF-Gmt</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Elongation factor G 1, mitochondrial</fullName>
            <shortName evidence="10">mEF-G 1</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Elongation factor G1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">MEF1</name>
        <name evidence="12" type="ordered locus">KLTH0G18942g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Mitochondrial GTPase that catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.</text>
    </comment>
    <comment type="pathway">
        <text evidence="10">Protein biosynthesis; polypeptide chain elongation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555850.1">
        <property type="nucleotide sequence ID" value="XM_002555804.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNQ2"/>
    <dbReference type="FunCoup" id="C5DNQ2">
        <property type="interactions" value="690"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNQ2"/>
    <dbReference type="GeneID" id="8294145"/>
    <dbReference type="KEGG" id="lth:KLTH0G18942g"/>
    <dbReference type="eggNOG" id="KOG0465">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002794_4_0_1"/>
    <dbReference type="InParanoid" id="C5DNQ2"/>
    <dbReference type="OMA" id="GQFAKVQ"/>
    <dbReference type="OrthoDB" id="198619at2759"/>
    <dbReference type="UniPathway" id="UPA00345"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="8 10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 10" id="KW-0547">Nucleotide-binding</keyword>
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    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Tr-type G" evidence="11">
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            <end position="351"/>
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    <feature type="binding site" evidence="10">
        <location>
            <begin position="79"/>
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        <ligand>
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        <source>
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    </evidence>
    <sequence length="763" mass="84555" checksum="3B377DFCCEEA20C3" modified="2009-07-28" version="1">MSFLRRAVSGHSYGPKTFGRMGLLNGSIMPILTTQRQVHASSVLRTYEEEKAVLDEIAPRLTERDLETSRKLRNIGISAHIDSGKTTFTERVLFYTGRIKAIHEVRGRDNVGAKMDSMDLEREKGITIQSAATYCSWDKDGQNYHFNLIDTPGHIDFTIEVERALRVLDGAVLVVCAVSGVQSQTVTVDRQMRRYNVPRVTFINKMDRMGANPFRAIEQINKKLKTPAAAIQVPIGAESELKGVVNIIDRVALYNEGANGEQIVEGPVPNELNELVEERRAMLIETLADVDDEIAEIFLEEQEPSTEQIKAAIRRATIARKFTPVLMGSALANRSVQPVLDAIVDYLPNPSEILNTGLDIANNEAKVNLVPSVQEPFVGLAFKLEEGKYGQLTYIRVYQGRLRKGGYITNVKTGKKIKVSRLVRMHSNDMEDVDEVGSGEICATFGIDCASGDTFTDGTLQYSMSSMYVPDAVISLSITPTSRDSTNFSKALNRFQKEDPTFRVRFDAESKETVISGMGELHLEIYVERMRREYNVDCVTGKPQVSYRESITIPAEFDYTHKKQSGGAGQYGRVMGSLSPLEGSNGNKFETAIVGGRIPDKYLAACGKGFEEACEKGPLIGHKVLSTHMLINDGAIHAVDSNELAFKTATMAAFKEAFLEAKPVILEPVMNVSVTAPNEFQGNVISLLNKLQAVIQDTENAQDEFTINAECSLNTLFGFATSLRSSTQGKGEFSLEFKHYSPTSPHLQRQLIADFEKKQQQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2R6</accession>
    <name>C5E2R6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0H07128p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0H07128g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
            <location evidence="1">Telomere</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556189.1">
        <property type="nucleotide sequence ID" value="XM_002556143.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2R6"/>
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        <property type="interactions" value="20"/>
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    <dbReference type="STRING" id="559295.C5E2R6"/>
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    <dbReference type="KEGG" id="lth:KLTH0H07128g"/>
    <dbReference type="eggNOG" id="ENOG502S0RQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_513904_0_0_1"/>
    <dbReference type="InParanoid" id="C5E2R6"/>
    <dbReference type="OMA" id="CVAGWKW"/>
    <dbReference type="OrthoDB" id="77828at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990879">
        <property type="term" value="C:CST complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016233">
        <property type="term" value="P:telomere capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.230">
        <property type="entry name" value="Stn1, C-terminal wHTH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024263">
        <property type="entry name" value="Stn1_C_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038240">
        <property type="entry name" value="Stn1_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018856">
        <property type="entry name" value="Stn1_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10451">
        <property type="entry name" value="Stn1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12659">
        <property type="entry name" value="Stn1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0779">Telomere</keyword>
    <feature type="domain" description="CST complex subunit Stn1 N-terminal" evidence="5">
        <location>
            <begin position="12"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Stn1 C-terminal fungi" evidence="6">
        <location>
            <begin position="293"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="243"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="247"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF10451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR30327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="464" mass="52676" checksum="A9996DBC4628D923" modified="2009-07-28" version="1">MANWDHVVYRNKDVDYYVPSLFHLSPTFQTTVPCLVCDLSYHFKRSKIITQKYYAGEPDAWLYGNYPIGSVEVLGCVAGWKWKLIGECDNAMFKLDDSTREPRGPPTLLSVKCSKGVVLRSGLPLGDLSGWTLKLAGQMNRFGELDVQSVRICSELQDEIEFWKRAMKWRRELSVPWTVEKAVLDRVCAPAGNAPPSSYNFIQWLEQQHYQREMKIADDYSEGPREIVLPFKHVSQDMVLGDQEAESEPHTGRHEQTQARSPEMHERSRAPELVETIDQAAGPRASVHDYVRHVLDHLLTQNRVEVSTLNTFRSPGPLRVLRRVAAQRARRQARPVDEVASSLFADVLQRLARAGLISVFAQGRILNLRVLKNCDDYVTQRIAALVELRSYTGKIDFRRACDLLGLARSAPLERLLVDLHKRALRRLAAASDTPLQSWWIELAGPAAAFVHFEYHHDPSSLAPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2S1</accession>
    <name>C5E2S1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0H07238p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0H07238g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the PET100 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556194.1">
        <property type="nucleotide sequence ID" value="XM_002556148.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2S1"/>
    <dbReference type="FunCoup" id="C5E2S1">
        <property type="interactions" value="34"/>
    </dbReference>
    <dbReference type="GeneID" id="8294511"/>
    <dbReference type="KEGG" id="lth:KLTH0H07238g"/>
    <dbReference type="eggNOG" id="ENOG502S1QV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_156745_0_0_1"/>
    <dbReference type="InParanoid" id="C5E2S1"/>
    <dbReference type="OMA" id="MKLPFRY"/>
    <dbReference type="OrthoDB" id="18175at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018625">
        <property type="entry name" value="Pet100"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33968">
        <property type="entry name" value="PROTEIN PET100 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33968:SF1">
        <property type="entry name" value="PROTEIN PET100 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09803">
        <property type="entry name" value="Pet100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR30332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="106" mass="12635" checksum="599D3F9FCE35DD73" modified="2009-07-28" version="1">MKLPFRYTRSQLEVFRFAFCLLSPVAIMYWIGIDTDKKLNVPGFWPDPETLNKIPKEPYEIKAELARMKKERLEKRLRLEKKIAEEYGIDIEAEKARIKEEMNSGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5E313</accession>
    <name>C5E313_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0H09460p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0H09460g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556286.1">
        <property type="nucleotide sequence ID" value="XM_002556240.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E313"/>
    <dbReference type="FunCoup" id="C5E313">
        <property type="interactions" value="837"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E313"/>
    <dbReference type="GeneID" id="8294611"/>
    <dbReference type="KEGG" id="lth:KLTH0H09460g"/>
    <dbReference type="eggNOG" id="KOG3010">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049344_1_2_1"/>
    <dbReference type="InParanoid" id="C5E313"/>
    <dbReference type="OMA" id="GPYWPAE"/>
    <dbReference type="OrthoDB" id="10027013at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051052">
        <property type="entry name" value="Diverse_substrate_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44942">
        <property type="entry name" value="METHYLTRANSF_11 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44942:SF4">
        <property type="entry name" value="METHYLTRANSFERASE TYPE 11 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="4">
        <location>
            <begin position="57"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR30424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="305" mass="35672" checksum="F2AD3CB5332B7593" modified="2009-07-28" version="1">MDHQRSRPKYIQLTREMSTYSENSFNSDFYQEKRPRYPESLYQEILSYHSGDLKLAIDVGCGTGISTLPLLKSFERVVGCDPSSTMLESAKKFKDKITVKDINRIEYSVCGAEDLTSLFASDSIDLVTGAESIHWVDEERFFKQAFQVLKPNGTLAYWFYVEPIFIEHPQANEIYEEFVYEDPAYMGPQWKPGKEKLRVYGEQIRIPEDKFTDIERHVYRPLVSKEKTAYFYGRDSMTVDDLRQYLRSWSAYHTWQQKFGEKGCDVAELLLDELKSKCGWDEANTRLKVEWGTAYYLARKKATTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5E371</accession>
    <name>C5E371_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0H10868p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0H10868g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556344.1">
        <property type="nucleotide sequence ID" value="XM_002556298.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E371"/>
    <dbReference type="FunCoup" id="C5E371">
        <property type="interactions" value="15"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E371"/>
    <dbReference type="GeneID" id="8294675"/>
    <dbReference type="KEGG" id="lth:KLTH0H10868g"/>
    <dbReference type="eggNOG" id="KOG1716">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_365223_0_0_1"/>
    <dbReference type="InParanoid" id="C5E371"/>
    <dbReference type="OMA" id="TVCIAEC"/>
    <dbReference type="OrthoDB" id="273181at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008138">
        <property type="term" value="F:protein tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033260">
        <property type="term" value="P:nuclear DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053239">
        <property type="entry name" value="Dual_spec_PTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47550">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE PPS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47550:SF1">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE PPS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="4">
        <location>
            <begin position="566"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="5">
        <location>
            <begin position="684"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR30482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="783" mass="88194" checksum="392202A5FC2AB2BB" modified="2009-07-28" version="1">MFYPAEPNGPGQPPNKRRKGHSEEQGSSFTDSASDKNHLIPGRVSLISHEKMRKLVAQHISQRLPDCDEVFPWLHGYSGATNPPHSCQWLAVIRSQPLSQGLIENSGLLKSSLDPHEFLMTLEPRKHHLESLLSNVGGILNLTSAEIKILTSACERHKLLPFLISDAKAQSWFGVGANKHSVGKSSSKHDPPRNWKQPGMFRRFDLQVAKFVEMSRNCVVYCLDQTTHYTSCPCCGLALLIHVARRIVDPKGNFRISVLDSTQIDPEWWGTPPMKVNALKKVHNSQLASDFDVASFNNWDRDLFYRERLEISKMSSASCISADASVWCGNSTDFEIYRLYNGDAMNAKPVNASVTSPDTVVTLPNLRSSNDDELIDTQLFNLPRPSKPWRLFLHCSESSCLPELSKVTSLIEHVQRKTPLAQTLISFPNSGSIGLGNLNLDSIKVILNMCLLLYSVGKHTNCGSLIYCSDGYTETSFLLVAFLIFVWDQPLDKVLVRLHGEVNRPFFLFPIDLQVLGHLQILLREQSPRRTEPESSSLDVDPELFSKMFFTKVSDNLNLLQLKGPLPSKILPHLYLGSLEHAQNPKLLQEIGIKNIVSVGETMPWLLAAISRKRSLTVSDINDQKTCTRPSISSASSASQIPSLQGSFLKRENGCAIIEENGFRVLHITNLDDNGEDHLLSQLDEVLSFIEECYNRQEKVLVHCMVGVSRSATVCIAECMRRLNCDVLRAYLYVRVRRLNIIIQPNLMFVYELCKWQETHGRPSHVDWHIICRAISELNSVYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3C1</accession>
    <name>C5E3C1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0H12122p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0H12122g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat LST8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556394.1">
        <property type="nucleotide sequence ID" value="XM_002556348.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3C1"/>
    <dbReference type="FunCoup" id="C5E3C1">
        <property type="interactions" value="678"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3C1"/>
    <dbReference type="GeneID" id="8294731"/>
    <dbReference type="KEGG" id="lth:KLTH0H12122g"/>
    <dbReference type="eggNOG" id="KOG0315">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_57_5_1"/>
    <dbReference type="InParanoid" id="C5E3C1"/>
    <dbReference type="OMA" id="VQRNYKH"/>
    <dbReference type="OrthoDB" id="400at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098588">
        <property type="term" value="C:bounding membrane of organelle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031931">
        <property type="term" value="C:TORC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031932">
        <property type="term" value="C:TORC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032956">
        <property type="term" value="P:regulation of actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031929">
        <property type="term" value="P:TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000339">
        <property type="entry name" value="WD-repeat protein pop3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037588">
        <property type="entry name" value="MLST8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19842">
        <property type="entry name" value="G BETA-LIKE PROTEIN GBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19842:SF0">
        <property type="entry name" value="TARGET OF RAPAMYCIN COMPLEX SUBUNIT LST8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="71"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="112"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="203"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="246"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR30532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="303" mass="33939" checksum="214CC97FD175E000" modified="2009-07-28" version="1">MSVILASAGYDHTIRFWEALTGVCSRTIQHSDSQVNRLEITSDKRFLAAAGHLNVRLYDIRTTNPNPVTSFEGHRGNVTSIAFQQENKWMVSSSEDGTIKVWDVRAPSVQRNYKHHAAVNEVVIHPNQGELISCDQDGNIKIWDLGENQCVHQLAPEDDTPLQSLSVASDGSMLVAGNNKGNCYVWQMPHQTDAANPRPVTKFRSHAKYITRVLLSSDVKHLATCSADHTARVWSIDDNFQLETTLDGHQRWVWDCAFSADSAYLVTACSDHYVRLWDLSTREIVRQYGGHHKGAICVALNDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3G3</accession>
    <name>C5E3G3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0H13310p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0H13310g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556436.1">
        <property type="nucleotide sequence ID" value="XM_002556390.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3G3"/>
    <dbReference type="FunCoup" id="C5E3G3">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3G3"/>
    <dbReference type="GeneID" id="8294795"/>
    <dbReference type="KEGG" id="lth:KLTH0H13310g"/>
    <dbReference type="eggNOG" id="ENOG502RYG1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026237_0_0_1"/>
    <dbReference type="InParanoid" id="C5E3G3"/>
    <dbReference type="OMA" id="HKPFTEL"/>
    <dbReference type="OrthoDB" id="2354757at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035838">
        <property type="term" value="C:growing cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051380">
        <property type="entry name" value="pH-response_reg_palI/RIM9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009571">
        <property type="entry name" value="SUR7/Rim9-like_fungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28013:SF8">
        <property type="entry name" value="AEL027WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28013">
        <property type="entry name" value="PROTEIN DCV1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06687">
        <property type="entry name" value="SUR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="89"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="121"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="155"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="182"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="243"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="325"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="403"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="543"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="251"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="349"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="367"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="409"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="551"/>
            <end position="565"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR30574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="594" mass="64644" checksum="07E64DD07FDE95C2" modified="2009-07-28" version="1">MAKFLNVGVSSAITVAQFVSMAFLIICCVTAPVFKQIGLSKADGIVFGTFGYCEQGSCTSASASYHPEQLATSESWKMGTTAREALGKILIIMPVAAGLSFFSMLGSFVSLFGAIGTSGAAFVINLLLSVIAFAGSALMCIVVFLLFYPNITWCGWLLIPAAAINLVCIPLIFLSYSLAPSKNQDSDAESDYTDRQGLTSLDQPPSKDLYEEDVFYNSSKKSALEKPAYPDFYKGPEIATSTTLNSGTTDSRSDREKLLNDKPLSNPVLKTAYGTAKIMKPVTEDAENDFSFENNTKETLPYSAIAGSQRDDHASIEMSAPAAAAMNGYRDPSSTSSFYSERDHRASRQDTLNTADLQPSSALNVDADAKKDKSSKDVLQHIIDNAIDEDDEEFIKQQTIDPSERPYLDEDDGIKDDDSDFTSVSQRGINPNYMPLSNGVKSTNYPPQPMKTHVPRTINYPSVPLGGAQVRPLVPLDHQRAPMNHPRMVAPQRVPHMYQPQQPVPRGYVAPQQGYRPSNAPTASEAVLTTNPDFMIPGVPSGGLGAKRAQRQNYAPPSHSVSSTHYRPAYKKRLPRQNMPPASMSRDGPYSGMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3R4</accession>
    <name>C5E3R4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NADH:ubiquinone reductase (non-electrogenic)</fullName>
            <ecNumber evidence="3">1.6.5.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0H15708g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a quinone + NADH + H(+) = a quinol + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:46160"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.6.5.9"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a ubiquinone + NADH + H(+) = a ubiquinol + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:23152"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NADH dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.6.5.9" evidence="3"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556537.1">
        <property type="nucleotide sequence ID" value="XM_002556491.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3R4"/>
    <dbReference type="FunCoup" id="C5E3R4">
        <property type="interactions" value="374"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3R4"/>
    <dbReference type="GeneID" id="8294903"/>
    <dbReference type="KEGG" id="lth:KLTH0H15708g"/>
    <dbReference type="eggNOG" id="KOG2495">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021377_1_0_1"/>
    <dbReference type="InParanoid" id="C5E3R4"/>
    <dbReference type="OMA" id="QIPAQKD"/>
    <dbReference type="OrthoDB" id="3244603at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050136">
        <property type="term" value="F:NADH dehydrogenase (quinone) (non-electrogenic) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015980">
        <property type="term" value="P:energy derivation by oxidation of organic compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.100:FF:000007">
        <property type="entry name" value="Rotenone-insensitive NADH-ubiquinone oxidoreductase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045024">
        <property type="entry name" value="NDH-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054585">
        <property type="entry name" value="NDH2-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43706:SF47">
        <property type="entry name" value="EXTERNAL NADH-UBIQUINONE OXIDOREDUCTASE 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43706">
        <property type="entry name" value="NADH DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22366">
        <property type="entry name" value="NDH2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="12">
        <location>
            <begin position="92"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="External alternative NADH-ubiquinone oxidoreductase-like C-terminal" evidence="13">
        <location>
            <begin position="471"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF22366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR30675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="538" mass="59395" checksum="064A5D65AD0F72DE" modified="2009-07-28" version="1">MFQLAKSSAFRSGAQRAFSRSFRAAQQQSAAAGGARKPASLARKIGKYTLYSVVGSVLAGTGYVSYKLYKEKNPSPQKPQSATFDNGSPRKTLVILGSGWGSVSLLKNLDTTLYNVVVVSPRNYFLFTPLLPSTPVGTIELKSIVEPVRSIARRAPGEVTYYEAEALDVDPVGKTVKVKSVSGSDADAVRDLKYDYLVVGVGAQPTTFGIPGVFENASFLKEIPDAQEIRVKVMNNIEKATTLPVSDPERKRLLNFVVVGGGPTGVEFAAELQDYIDQDLSKWMPELSKELHVTLVEALPNILNMFDKSLIKYAEDLFQETKINLRTNTMVKNVTPTVITAKCGDDIEDIPYGVLVWATGNAPREVSKSLMNRMECQNSRRGLLINEKLQLLGAEDSIWAIGDCTFYPGLFPTAQVAHQEGEYLASVLKKQYKIDQLKWHTLQNSNAAENDKLKSKVEKLSSQIKAFKYNHHGALAYIGSEKAIADLALGESMYHLAGSFTFLFWKSAYLNMCLSFRNRLLVALDWCKVSFFGRDSSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>C5DB85</accession>
    <name>C5DB85_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2-dehydropantoate 2-reductase</fullName>
            <ecNumber evidence="4">1.1.1.169</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Ketopantoate reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0A00506g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the NADPH-dependent reduction of ketopantoate into pantoic acid.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>(R)-pantoate + NADP(+) = 2-dehydropantoate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16233"/>
            <dbReference type="ChEBI" id="CHEBI:11561"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15980"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.169"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the ketopantoate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.169" evidence="4"/>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551484.1">
        <property type="nucleotide sequence ID" value="XM_002551438.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DB85"/>
    <dbReference type="FunCoup" id="C5DB85">
        <property type="interactions" value="51"/>
    </dbReference>
    <dbReference type="GeneID" id="8290275"/>
    <dbReference type="KEGG" id="lth:KLTH0A00506g"/>
    <dbReference type="eggNOG" id="ENOG502QWBM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031468_2_1_1"/>
    <dbReference type="InParanoid" id="C5DB85"/>
    <dbReference type="OMA" id="FKPSMLQ"/>
    <dbReference type="OrthoDB" id="3609at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008677">
        <property type="term" value="F:2-dehydropantoate 2-reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015940">
        <property type="term" value="P:pantothenate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.10:FF:000017">
        <property type="entry name" value="2-dehydropantoate 2-reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003710">
        <property type="entry name" value="ApbA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013752">
        <property type="entry name" value="KPA_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051402">
        <property type="entry name" value="KPR-Related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013332">
        <property type="entry name" value="KPR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00745">
        <property type="entry name" value="apbA_panE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21708:SF30">
        <property type="entry name" value="2-DEHYDROPANTOATE 2-REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21708">
        <property type="entry name" value="PROBABLE 2-DEHYDROPANTOATE 2-REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02558">
        <property type="entry name" value="ApbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08546">
        <property type="entry name" value="ApbA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0521">NADP</keyword>
    <keyword evidence="3 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketopantoate reductase N-terminal" evidence="5">
        <location>
            <begin position="7"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Ketopantoate reductase C-terminal" evidence="6">
        <location>
            <begin position="192"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR21042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="334" mass="36846" checksum="E7DBD072ABAF9C5F" modified="2009-07-28" version="1">MPNKPNVLLVGAGGVGAMASFALEYSGESSVTSVLRSDYAHVVENGFKINSCDYGRGIRYRPSNVVNSIEASKQYGPFDYVVLATKCIPGVSSMIDIIAPAVSKDTAIVLIQNGIGIEDEALFKCPGNVILSGVSMIASSNVKGHIEHSDRDFLSVGYFENVNLTNEHQEKVCKRFVSMYKNDKNQCVFDENVKYSRWRKLVYNACLNPVCALTEADVGRLEIFGGTDSIIRKAMKEVIAIAKSDGVELSEALMDFMIRSKDPVYYRPSMLVDIDKGNPLEVEVICGNPVRIAKKNGVEAPYLTLIYDLLKVVQGRIMEKRGMLIVPKERQVPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBD3</accession>
    <name>C5DBD3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0A01628p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0A01628g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551532.1">
        <property type="nucleotide sequence ID" value="XM_002551486.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBD3"/>
    <dbReference type="GeneID" id="8290619"/>
    <dbReference type="KEGG" id="lth:KLTH0A01628g"/>
    <dbReference type="eggNOG" id="ENOG502S8V1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066755_0_0_1"/>
    <dbReference type="InParanoid" id="C5DBD3"/>
    <dbReference type="OMA" id="IVFKWRH"/>
    <dbReference type="OrthoDB" id="65445at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053218">
        <property type="entry name" value="Pathogen-related_defense"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31723:SF10">
        <property type="entry name" value="PATHOGEN-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31723">
        <property type="entry name" value="PATHOGENESIS-RELATED FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR21090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="215" mass="24417" checksum="CE73C5BD7CFECAF5" modified="2009-07-28" version="1">MTEQSTPLWLQDRDAVIAQTPAHMWRLGKPTYKFTDRRLNRERQVHFEPGSLGDLVTNLARVFEMEATNKANPDDWISVDPRVFRMTVNGSREYTVQDIVTKGGYNVFLGDLPNYKASESDYAKSEDAFHKAFRTGFLWEILTLEAELPVATFTWRHWGQQNGVFNGHKGDGSVLDIKGTTRATLNDKLQLVRLEHTFDAPGMIDTLSGVCPMAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBJ0</accession>
    <name>C5DBJ0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">KLTH0A03036p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0A03036g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551589.1">
        <property type="nucleotide sequence ID" value="XM_002551543.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBJ0"/>
    <dbReference type="STRING" id="559295.C5DBJ0"/>
    <dbReference type="GeneID" id="8290389"/>
    <dbReference type="KEGG" id="lth:KLTH0A03036g"/>
    <dbReference type="eggNOG" id="KOG1601">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_415082_0_0_1"/>
    <dbReference type="InParanoid" id="C5DBJ0"/>
    <dbReference type="OMA" id="EALWRMY"/>
    <dbReference type="OrthoDB" id="515401at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000007">
        <property type="entry name" value="Nitrogen regulatory AreA, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013860">
        <property type="entry name" value="AreA_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039355">
        <property type="entry name" value="Transcription_factor_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10071:SF281">
        <property type="entry name" value="BOX A-BINDING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10071">
        <property type="entry name" value="TRANSCRIPTION FACTOR GATA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08550">
        <property type="entry name" value="GATA_AreA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00619">
        <property type="entry name" value="GATAZNFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="10">
        <location>
            <begin position="455"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="125"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="371"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="506"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="642"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="165"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="377"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="387"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="403"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="512"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="526"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="539"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="585"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="642"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="658"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="681"/>
            <end position="690"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR21147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="720" mass="77455" checksum="00CA1ED9267811F8" modified="2009-07-28" version="1">MTERKEDARSRERQCQKKLQEAISDSSAEALWHMYSSAQASLPYRDRMVNLTWRMLGARMRKNVPVARQDQDQLYPEQVKYLSQSDKSSPMVPEEIDYMAELREIRGQSLSTAASSTLNMQATDGLAEGRASSSSASAPVKTEPLESKLSISDQIVSGMGRGPTSGPTPSNSMKPFSQRDMEPSALGFDDNVHFGSFSNISQHVGFMADVDVDSFNILDGRESASGRELSHEEDEDMGMRAATYSSPALISDSGVSSADNPSYGQQSMSNSTLLNNMDQASALSTVPNSVTIGGLNSSILGGQTFFDDHDFLHPVNEGNQDNENIFPPLHATNSQISLPDLYDRTSNITPISIRRPSSAWQALPNGLGASAPGTSAIAPSSLPNPQVSRRPVSVNSVRKKQIKSSNSRRGSLSFTSINGGSIPTGFVGQQEKGSNDAARKPTTNEGTSGASASGGKTDTKCTNCHTKTTPLWRRDPQGNPLCNACGLFLKLHGVVRPLSLKTDVIKKRQRSSNKATAANSSAVVGQDNSPSKDAVQNSKSKRPLSRKKTSSVNILGEARPSLPRSQSSFSRRKSKASFNTRDAENASSESGSGTPTPVQASGADTQTMSNTASEMDIDQHSTANVDEGTKHNESSFDLILEQWSQPTNSENNSGRTHPQQGSQPQSKQPEGQSAFYFSSSHGREGRRKPEPASGLSGPQNPADKKNAGENANWDWLTLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBK7</accession>
    <name>C5DBK7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">KLTH0A03410p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0A03410g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551606.1">
        <property type="nucleotide sequence ID" value="XM_002551560.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBK7"/>
    <dbReference type="FunCoup" id="C5DBK7">
        <property type="interactions" value="551"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBK7"/>
    <dbReference type="GeneID" id="8290406"/>
    <dbReference type="KEGG" id="lth:KLTH0A03410g"/>
    <dbReference type="eggNOG" id="KOG0385">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000315_17_3_1"/>
    <dbReference type="InParanoid" id="C5DBK7"/>
    <dbReference type="OMA" id="WNICRID"/>
    <dbReference type="OrthoDB" id="5857104at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000015">
        <property type="entry name" value="lymphoid-specific helicase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10799">
        <property type="entry name" value="SNF2/RAD54 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="198"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="565"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="134"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="78"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="134"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR21164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="806" mass="93200" checksum="FD18B23CE0E70677" modified="2009-07-28" version="1">MVKTRNASRRSQRVDYKEKQESNLEPDTLVSSESSDDEEKGDAPIWLSENNELGVQDVDLSSGDEEDDTELNNLIQEQESKRQEAQLKKDEEEKSEEEFDDTTVSMKLNRLQEFVKQSQVYSRIIADTLLTRSQERQTEKEAKEPPRKRTKVTDFFRKSKKEPVADSEEPEPVLHQQPSMLKNCQLKPYQLEGVNWLITLYENGLNGILADEMGLGKTLQSIALLAFITEMDTGGPFLIAAPLSTLDTWVNEFSRFAPDIKVLKYYSSQGQRQRQQLLKDFFRKTKNEGVIVTSYEVIIRDIEQILSYQWKFLIVDEGHRIKNINCKLIRELKRINTLNRLLITGTALQNNLSELWSLLNFIMPDIFADFEIFHKWFDFSDLELKSSSQGLNRLINDELEKNLISNLHTILKPFLLRRLKKVVLAKSLPPKREYIVNCPLTPIQRKFYKAALGGKLKQAIFKQAIKNFFTLNQDQIGCVSNKSIREFLEWRLKHGREEPETKVLRQMNELYNQHIHRALLHKRLQNPMIQLRQVVDSTLLFFFPFLDPEHLTLEFLLQTSGKLQILQQLVPPLVAKKHKVLIFTQFVSMLDLIEDWCELNEIRVCRIDGSMDNEARQEHIERFNDGHDSYDAFLISTRAGGLGINLTAADTVVLFDSDWNPQVDLQAMDRTHRIGQTKPVIVYRLCCDNTVEHVILTRAASKRKLEKMVIQLGNFGTLKRLAGNESSFISQGHTPANSNKELMHELSQLLMSDESSISGFPRSGNPELTTDELAELFDRSEKAFRIENAKKEWPHIRLFESTTAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBN3</accession>
    <name>C5DBN3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0A04048p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0A04048g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551632.1">
        <property type="nucleotide sequence ID" value="XM_002551586.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBN3"/>
    <dbReference type="FunCoup" id="C5DBN3">
        <property type="interactions" value="178"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBN3"/>
    <dbReference type="GeneID" id="8290435"/>
    <dbReference type="KEGG" id="lth:KLTH0A04048g"/>
    <dbReference type="eggNOG" id="KOG0703">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023062_3_1_1"/>
    <dbReference type="InParanoid" id="C5DBN3"/>
    <dbReference type="OMA" id="YEYKKWI"/>
    <dbReference type="OrthoDB" id="10266696at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.150:FF:000009">
        <property type="entry name" value="stromal membrane-associated protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051718">
        <property type="entry name" value="ARF_GTPase-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45705">
        <property type="entry name" value="FI20236P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45705:SF1">
        <property type="entry name" value="FI20236P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Arf-GAP" evidence="7">
        <location>
            <begin position="24"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="140"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="228"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="157"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="170"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="228"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="268"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR21190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="291" mass="30820" checksum="53BBC374B50E6D53" modified="2009-07-28" version="1">MSNFLWKLQKSGSRIGPMSSPAIKKALAALLRDPNNSTCADCKTAGHPRWASWSLGVFICIKCAGIHRSLGTHISKVKSVDLDTWQEEHMRKVVEFGNNAAANAVYECKLSGNHTPEASKIADFIRNKYELKKWIGNAAEVSAPPSRPKTVEKKPQVAQQAHGSQTSLVSQTSSAPSRSATSSQVDLNLASPSVASRTTLNAAAREPSGRPDLKKSILSLYSRPRTSTASVASGASNASFSSSTGTPSIASNSTPAAGYPLPGASFSLPAQTNTTSSASLEDNDLFKNVWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBN4</accession>
    <name>C5DBN4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="15">KLTH0A04070p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0A04070g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Transport of phosphate groups from the cytosol to the mitochondrial matrix.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551633.1">
        <property type="nucleotide sequence ID" value="XM_002551587.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBN4"/>
    <dbReference type="FunCoup" id="C5DBN4">
        <property type="interactions" value="846"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBN4"/>
    <dbReference type="GeneID" id="8290436"/>
    <dbReference type="KEGG" id="lth:KLTH0A04070g"/>
    <dbReference type="eggNOG" id="KOG0767">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039456_2_0_1"/>
    <dbReference type="InParanoid" id="C5DBN4"/>
    <dbReference type="OMA" id="YKGAIWL"/>
    <dbReference type="OrthoDB" id="427452at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035434">
        <property type="term" value="P:copper ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990547">
        <property type="term" value="P:mitochondrial phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000076">
        <property type="entry name" value="Mitochondrial phosphate carrier protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000131">
        <property type="entry name" value="Mitochondrial phosphate carrier protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044677">
        <property type="entry name" value="SLC25A3/Pic2/Mir1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45671">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25 (MITOCHONDRIAL CARRIER PHOSPHATE CARRIER), MEMBER 3, LIKE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45671:SF10">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25 MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="257"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="315"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="55"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="153"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="255"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="13"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00054508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR21191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="346" mass="38311" checksum="DD98A3DF91DB8C10" modified="2009-07-28" version="1">MVRSQLRDYISERPRQLPGRLPVHPLEPRSRPRPHSALMSQNSSIFNSPKLYSPDYYAACTIGGLVACGPTHSAVTPLDLVKCRRQVDSSLYKSNLEGWRQIIKTEGGVGKIFTGVGATAIGYSLQGAFKYGGYEFFKHQYSQLVSPETAHQYRTGIFLAASASAEFIADLFLCPWEAIKVRQQTAVPPPFARNVFEAYSKMVGAEGFASLYKGITPLWCRQIPYTMCKFTSFERIVEMIYARLPTPKREMSQLGQIGVSFAGGYLAGILCAVVSHPADVMVSKVNNERKKGESMMEATSRIYAKIGFPGLWNGLAVRILMIGTLTSFQWLIYDSFKATVGLPTTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBQ4</accession>
    <name>C5DBQ4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="18">KLTH0A04532p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">KLTH0A04532g</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002551607.1"/>
    </dbReference>
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        <property type="interactions" value="444"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBQ4"/>
    <dbReference type="GeneID" id="8290457"/>
    <dbReference type="KEGG" id="lth:KLTH0A04532g"/>
    <dbReference type="eggNOG" id="KOG2068">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028046_0_0_1"/>
    <dbReference type="InParanoid" id="C5DBQ4"/>
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    <dbReference type="OrthoDB" id="1923159at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
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        <property type="term" value="C:CCR4-NOT core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="P:positive regulation of macromolecule biosynthetic process"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Mot2"/>
    </dbReference>
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        <property type="entry name" value="NOT4_mRING-HC-C4C4"/>
    </dbReference>
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        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
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        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
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        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
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        <property type="entry name" value="Znf_RING"/>
    </dbReference>
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        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14570">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="6 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="15">
        <location>
            <begin position="33"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="16">
        <location>
            <begin position="137"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="17">
        <location>
            <begin position="231"/>
            <end position="258"/>
        </location>
    </feature>
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        <location>
            <begin position="231"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="278"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="559"/>
            <end position="597"/>
        </location>
    </feature>
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        <location>
            <begin position="98"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="318"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="568"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="583"/>
            <end position="597"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
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        </source>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBZ8</accession>
    <name>C5DBZ8_LACTC</name>
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        <recommendedName>
            <fullName evidence="2">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="2">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0A06666g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
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            </authorList>
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            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
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            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_002551701.1"/>
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    <dbReference type="GeneID" id="8290554"/>
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    <dbReference type="eggNOG" id="KOG0880">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="InParanoid" id="C5DBZ8"/>
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    <dbReference type="OrthoDB" id="193499at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
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        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016018">
        <property type="term" value="F:cyclosporin A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00317">
        <property type="entry name" value="cyclophilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF568">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE CPR4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0697">Rotamase</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002947755" description="peptidylprolyl isomerase" evidence="6">
        <location>
            <begin position="20"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="273"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="7">
        <location>
            <begin position="44"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR21305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="313" mass="35649" checksum="22AE9EEFB2CDEF99" modified="2009-07-28" version="1">MLVNYVLWVLSALMCFAKAATIDDAKLYAPNPPVTHRAFFTIEYFNNATQQVEETDVTIELYGTVVPKTVDNFVKLAKGVTAVMKGKDEKKDRFTLGYKNTLFHRIINDFMIQGGDVLPDVGPFNTFGGMFFDDENFDLKHDRPGRLSMANINKPDTNASQFFIVTSTRALDELDGKHVVFGQVVAGLEGLLEKVQFVETTKDEYKPKHDVKLKYSLVEDLQISNKEQLHEKYLQDLLAFQNGDRSKGLTMSTTIKQGAKEEKIMRELRYEDLHHPLVKVVLGMVLISAIYVVSKNRKRIFSKTTKIVSMRHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DC16</accession>
    <name>C5DC16_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein uS19m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0A07062g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the universal ribosomal protein uS19 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551765.1">
        <property type="nucleotide sequence ID" value="XM_002551719.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DC16"/>
    <dbReference type="FunCoup" id="C5DC16">
        <property type="interactions" value="166"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DC16"/>
    <dbReference type="GeneID" id="8290572"/>
    <dbReference type="KEGG" id="lth:KLTH0A07062g"/>
    <dbReference type="eggNOG" id="KOG0899">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_144911_1_0_1"/>
    <dbReference type="InParanoid" id="C5DC16"/>
    <dbReference type="OMA" id="EYVAFEV"/>
    <dbReference type="OrthoDB" id="2043at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.860.10:FF:000001">
        <property type="entry name" value="30S ribosomal protein S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.860.10">
        <property type="entry name" value="30s Ribosomal Protein S19, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00531">
        <property type="entry name" value="Ribosomal_uS19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002222">
        <property type="entry name" value="Ribosomal_uS19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020934">
        <property type="entry name" value="Ribosomal_uS19_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023575">
        <property type="entry name" value="Ribosomal_uS19_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880">
        <property type="entry name" value="RIBOSOMAL PROTEIN S19P FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880:SF8">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US19M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00203">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002144">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00975">
        <property type="entry name" value="RIBOSOMALS19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54570">
        <property type="entry name" value="Ribosomal protein S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00323">
        <property type="entry name" value="RIBOSOMAL_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00044183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR21323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="91" mass="10163" checksum="DA0412DC3DA8503B" modified="2009-07-28" version="1">MQFTLSLLSRSVWKGPNIVPLPVKEALAKGAPIRTNARSATILPQFVGLKFQVHNGKEYVAFEVSEDMVGSKLGEFAPTRKRFSYTHTKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>C5DC49</accession>
    <name>C5DC49_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0A07810p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0A07810g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551798.1">
        <property type="nucleotide sequence ID" value="XM_002551752.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DC49"/>
    <dbReference type="FunCoup" id="C5DC49">
        <property type="interactions" value="39"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DC49"/>
    <dbReference type="GeneID" id="8290606"/>
    <dbReference type="KEGG" id="lth:KLTH0A07810g"/>
    <dbReference type="eggNOG" id="ENOG502QRDE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023620_2_0_1"/>
    <dbReference type="InParanoid" id="C5DC49"/>
    <dbReference type="OMA" id="HCIDNGV"/>
    <dbReference type="OrthoDB" id="5595695at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01299">
        <property type="entry name" value="Met_dep_hydrolase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051781">
        <property type="entry name" value="Metallo-dep_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057744">
        <property type="entry name" value="OTAase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43135">
        <property type="entry name" value="ALPHA-D-RIBOSE 1-METHYLPHOSPHONATE 5-TRIPHOSPHATE DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43135:SF3">
        <property type="entry name" value="ALPHA-D-RIBOSE 1-METHYLPHOSPHONATE 5-TRIPHOSPHATE DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="1">
        <location>
            <begin position="86"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="441" mass="47767" checksum="4A30316CD941322D" modified="2009-07-28" version="1">MSSCAIDVNERNNKKLEDLAVPWRFKPLQKYCIKNSNLVDVVEGTTFPEAYIFVENGKISKVEFGSGQPVDVDTNEYQIIDGTGKYVTPGLFDNHVHMASVTGELDLGKLMTMPKTVSLLRVRYTMEASLARGFTTVRDCGGAESYIKAAVNDGSVIGPRMITCGHAISQTGGHGDLRPGNLPASAFESCSCHYGQVGVVADGVGECYRAAREEFRRGADFIKMMAGGGVASPTDKVTNVQFCHDEIRALVDVANTYHTYVTAHAYTAEAIQNCIKLGVRGIEHGNLLDEETAELMAKLGCYLTPTLVTYKIMASDQFGSFLGPVNSQKNAEVLSQGIDALKIAQSKGVKICYGSDLLGPLAGYQTQEFFIRGKVQTAQEVLLSATVTPAEMNGLGDKLGQIKPGFIADLLLMNANPLDDISILDEPETNLFLVMKEGRIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>C5DC60</accession>
    <name>C5DC60_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0B00264p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0B00264g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551809.1">
        <property type="nucleotide sequence ID" value="XM_002551763.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DC60"/>
    <dbReference type="FunCoup" id="C5DC60">
        <property type="interactions" value="39"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DC60"/>
    <dbReference type="GeneID" id="8290628"/>
    <dbReference type="KEGG" id="lth:KLTH0B00264g"/>
    <dbReference type="eggNOG" id="ENOG502QRDE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023620_2_0_1"/>
    <dbReference type="InParanoid" id="C5DC60"/>
    <dbReference type="OMA" id="DVFPGPR"/>
    <dbReference type="OrthoDB" id="5595695at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01299">
        <property type="entry name" value="Met_dep_hydrolase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051781">
        <property type="entry name" value="Metallo-dep_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057744">
        <property type="entry name" value="OTAase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43135">
        <property type="entry name" value="ALPHA-D-RIBOSE 1-METHYLPHOSPHONATE 5-TRIPHOSPHATE DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43135:SF3">
        <property type="entry name" value="ALPHA-D-RIBOSE 1-METHYLPHOSPHONATE 5-TRIPHOSPHATE DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="1">
        <location>
            <begin position="86"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="441" mass="48028" checksum="45FC3FFC40C44E48" modified="2009-07-28" version="1">MQLTAIDVNERNNKSLEDLVTPWKIPPLKDYYIKNANLVDVREGITKPGAHIFTSGGFITNICFSDHEADKCTSDKFEIIDATGKFVMPGLFDNHVHVTSVPGETDLSKTLNMPKTRALMRVRHKLEGALARGFTTLRGCGGAESYIKAAVIEGSVIGPRLLTCGHAISQTGGHGDMRPADLPGEAFDSCSCHMNDLGVVADGAAECYKVAREELRRGADFIKIMAGGGVASPTDKITNLQFCDDEVTALVKVAKTYNTYVTAHAYTPEAIKACVSYGVKGIEHGNLLDEETAQLMAKAGCYLTPTLVTYKIMASDQFSTFLSGSNREKNTQVLYRGIEALRIAKKNGVKICYGSDLLGSLGGYQTQEFFIRGRYLSTHEVLLSATITPAEMNGLQDQIGEIKPGFIADMLLMNSNPLENITVLDEPESNLVFVMKEGRVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>C5DC77</accession>
    <name>C5DC77_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0B00704p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0B00704g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551826.1">
        <property type="nucleotide sequence ID" value="XM_002551780.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DC77"/>
    <dbReference type="GeneID" id="8290648"/>
    <dbReference type="KEGG" id="lth:KLTH0B00704g"/>
    <dbReference type="eggNOG" id="ENOG502SX82">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2050076_0_0_1"/>
    <dbReference type="InParanoid" id="C5DC77"/>
    <dbReference type="OrthoDB" id="10325852at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR21388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="121" mass="13018" checksum="3320164BF2D0A3B1" modified="2009-07-28" version="1">MNSTAEQSQIIIPCGRIYNLTVTSNTSVLEGWCCGIAASSGADDREQTLSHEFSSISCGDNTMLQITANTTVMDGYFCGSGMANPSNAGQSSLTQTNAASDLAPRWTQFFILLSFFMQYFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCF7</accession>
    <name>C5DCF7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0B02684p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0B02684g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551906.1">
        <property type="nucleotide sequence ID" value="XM_002551860.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCF7"/>
    <dbReference type="FunCoup" id="C5DCF7">
        <property type="interactions" value="218"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCF7"/>
    <dbReference type="GeneID" id="8290737"/>
    <dbReference type="KEGG" id="lth:KLTH0B02684g"/>
    <dbReference type="eggNOG" id="KOG0519">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008307_3_0_1"/>
    <dbReference type="InParanoid" id="C5DCF7"/>
    <dbReference type="OMA" id="WREINDN"/>
    <dbReference type="OrthoDB" id="21225at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000156">
        <property type="term" value="F:phosphorelay response regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000146">
        <property type="entry name" value="Putative two-component response regulator SSK1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45339">
        <property type="entry name" value="HYBRID SIGNAL TRANSDUCTION HISTIDINE KINASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45339:SF1">
        <property type="entry name" value="HYBRID SIGNAL TRANSDUCTION HISTIDINE KINASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="407"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="138"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="569"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="571"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="3">
        <location>
            <position position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR21468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="595" mass="64759" checksum="A9BCDDD28DEB6A8E" modified="2009-07-28" version="1">MAQKVGFRSPVPSPSGLAVQVPHKEIRLKVWVRLDSQKLPPDTINQATAIEFEREDTIDDLKDRIFRRFRDTRWAQENDSFGIAIGCWCECGFGATSAGDLKDLLLQPVPNMASNAAPIDLSHAPPYIRRTSRTCSPHAATLGTPPSYSPHPAHPSYNKLQCSTQSSTPLAQLRLSVGAGNKELGGGMDGCALSPVRLGSARRLSRTATPAVGEVDPDSIPHQSLLEPDELVFNVCKNLFGGVAAQTANDALVVFSNNPDIPLFDETMDGAPMVEDLTDYKLVVDEEQLRKLSHTNDEENIDCPKQAILLLPRGFEGDVNLPSPNVATPSASSPPALEEVSPGAAISPTQRVSPVLMTSAPLVEEEIGLVPPRDPIVQREGSPFILSAMESVTTESITRDKVFPKISVLVVEDNMINQAILSSFLRKHKIHYKVAKNGVEAVDRWKEGGMHLILMDLEMPLLSGIDAAKEIRKLEKQNGIGTGDPLGKHAELKGLTCKAPVIIVALTASNSQTDKTEALLAGCNDYLTKPVNLDWLTRKITEWGCMQALIDFDDWKDNDERLNSCIEQKAKASQKTTSTSNASKSTNLNKRITTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCT0</accession>
    <name>C5DCT0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0B05544p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0B05544g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552029.1">
        <property type="nucleotide sequence ID" value="XM_002551983.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCT0"/>
    <dbReference type="FunCoup" id="C5DCT0">
        <property type="interactions" value="1606"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCT0"/>
    <dbReference type="GeneID" id="8290867"/>
    <dbReference type="KEGG" id="lth:KLTH0B05544g"/>
    <dbReference type="eggNOG" id="ENOG502R1FS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015202_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCT0"/>
    <dbReference type="OMA" id="PMDISFC"/>
    <dbReference type="OrthoDB" id="2288358at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000228">
        <property type="term" value="C:nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1390:FF:000005">
        <property type="entry name" value="Meiosis-specific transcription factor NDT80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1390">
        <property type="entry name" value="NDT80 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052605">
        <property type="entry name" value="Fungal_trans_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024061">
        <property type="entry name" value="NDT80_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037141">
        <property type="entry name" value="NDT80_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35144">
        <property type="entry name" value="MEIOSIS-SPECIFIC TRANSCRIPTION FACTOR NDT80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35144:SF2">
        <property type="entry name" value="MEIOSIS-SPECIFIC TRANSCRIPTION FACTOR NDT80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05224">
        <property type="entry name" value="NDT80_PhoG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51517">
        <property type="entry name" value="NDT80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NDT80" evidence="4">
        <location>
            <begin position="96"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="NDT80" evidence="2">
        <location>
            <begin position="96"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="40"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="418"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="51"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="62"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="467"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR21591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="725" mass="80667" checksum="B5ECA4E15CFAF740" modified="2009-07-28" version="1">MPDDANEETIQEFAYSLNAEDGLKDIAGGEVHDDLNEAIVENDSRGKVENSGGSERSAQQRRTGKRRKAHGRMRNFVYNQESSSSRPHCRGVLSAKEKSENNVETITHEDGSTSNYFDKRRLKIAPRSTLQFKIGPSFETVGRYCQVLGHRDKRPVNFMLRPRIDRGFDHIEDEWIGYKRNYFTLVTSFEVESLELPEFLNQSFEVDGVSGASSPLRPKYFAVRLMAVCDEDESQVSLVQHTAKRDKGPQFEPPILALVPATLPSHQIIREASNVRNESKMKKYDSLFFFYRNTDAQEFAPESVVHSYPKDRIKKVARYERVQFSSSINAKKQPNQCKHFKLRVVLGCVVDGQHLNQDSFCAYQGLDCLVENGTGTFVPILEMETPPLVIRGRSPSNYSAALATPSPKRAKACQNSISATRNSGPGKGVPTCGPLTRGTPRAVFKASEFQDSNEDGMQKRRGATKPLSFSSSNLKPSASVKTPDSNMPKKTPSSLTFSEKRLDMQTMSSIEAVMMRHLPHILERGSVVEGLDLLSKHCSDSSYTIGMRDIELTPFLLGTPQDFGGHHSTSGFESDVFGLGMSPEPAHPHSPSNACTIRETTKQLRPLRERKLNNKPASMDITFSGKPSSTNNDEATECTFSENSYQFHATINAKSSRGTANVPRSVEPNPMDISFRLNMGTDSASMPRPISNTGLASRRMLTASGKPSDIFGASELFDEPSFYRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>C5DD67</accession>
    <name>C5DD67_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">KLTH0B08712p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0B08712g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552166.1">
        <property type="nucleotide sequence ID" value="XM_002552120.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DD67"/>
    <dbReference type="FunCoup" id="C5DD67">
        <property type="interactions" value="885"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DD67"/>
    <dbReference type="GeneID" id="8291007"/>
    <dbReference type="KEGG" id="lth:KLTH0B08712g"/>
    <dbReference type="eggNOG" id="KOG0761">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015166_0_0_1"/>
    <dbReference type="InParanoid" id="C5DD67"/>
    <dbReference type="OMA" id="FVSPIEM"/>
    <dbReference type="OrthoDB" id="1747031at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990542">
        <property type="term" value="P:mitochondrial transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045315">
        <property type="entry name" value="Mtm1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45760">
        <property type="entry name" value="FI19922P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45760:SF2">
        <property type="entry name" value="FI19922P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="104"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="12"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="139"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="250"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR21728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="344" mass="38329" checksum="D5F4F84B2899FD2A" modified="2009-07-28" version="1">MSNEKKEGIGLKERLLSAVAGSLLTSLILTPMDVVRIRLQQQKMLPSCDCDANGKLSLKTTAPKGVFWQDACFADVHCQTSSVNYNSTWDAFTKIARIEGARSLWRGLSITLVMAVPANMVYFIGYESLRDNSFLQNKYSRLNPLLCGAIARVLAATTVAPLELFRTRLQSIPRSSPNSTAAMMVKDLIKESRYEISRVGYKALFRGLEITLWRDVPFSSIYWGCYEFYKSNISIKSERSIVNSSNTNWNHFVNSFVGGSFGGAVAAILTHPFDVGKTRMQISFLNNTPGKSPSKNMFKYLNQIRKSEGLGALYTGLVPRVIKIAPSCAIMISTYEVCKRLFST</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>C5DD91</accession>
    <name>C5DD91_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0B09284p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0B09284g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the lipid-translocating exporter (LTE) (TC 9.A.26.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552190.1">
        <property type="nucleotide sequence ID" value="XM_002552144.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DD91"/>
    <dbReference type="GeneID" id="8291033"/>
    <dbReference type="KEGG" id="lth:KLTH0B09284g"/>
    <dbReference type="eggNOG" id="ENOG502QURG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033465_3_1_1"/>
    <dbReference type="InParanoid" id="C5DD91"/>
    <dbReference type="OMA" id="EKHICIS"/>
    <dbReference type="OrthoDB" id="3358017at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007568">
        <property type="entry name" value="RTA1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31465">
        <property type="entry name" value="PROTEIN RTA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31465:SF1">
        <property type="entry name" value="PROTEIN RTA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04479">
        <property type="entry name" value="RTA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="121"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="151"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="194"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="227"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="272"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="305"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR21752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="359" mass="39375" checksum="6EB986565B62D75B" modified="2009-07-28" version="1">MSATVKEKVMFAATVSLKPSSYTAANGTIVHYLQRPYYTYTPTVGGAAVFAVLFGISLVVLSVQLWQYSKQRTSMERQIMADGSVSRDQTEDVESHDDGGLGLAAPAAVQPCSNARLIGKFVPLMVGIAVEIGGYAARIASRNDLYSIGPYVAQAVMLLVAPALMAATIYMNFGRLLTLLRATQLSIVPARFNTTIFVMGDILSFVLQGAGGGILGASDNHQLGSNIVIGGLAIQVFVFGFFVATEVRFLMCARRMSPVAYRISRHWKVLNINLFVSSLLILARSIVRLVEFAQGFNGYIMEHEWFIYVFDAVPMFLVVVSFIVFFNNGNLFRVEFECTSMISDSRKYSMDTMTGCIDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDJ9</accession>
    <name>C5DDJ9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0C01584p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0C01584g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552298.1">
        <property type="nucleotide sequence ID" value="XM_002552252.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDJ9"/>
    <dbReference type="FunCoup" id="C5DDJ9">
        <property type="interactions" value="91"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDJ9"/>
    <dbReference type="GeneID" id="8291156"/>
    <dbReference type="KEGG" id="lth:KLTH0C01584g"/>
    <dbReference type="eggNOG" id="KOG2043">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002149_0_0_1"/>
    <dbReference type="InParanoid" id="C5DDJ9"/>
    <dbReference type="OMA" id="SWLYHLI"/>
    <dbReference type="OrthoDB" id="342264at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035361">
        <property type="term" value="C:Cul8-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990683">
        <property type="term" value="P:DNA double-strand break attachment to nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00027">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053036">
        <property type="entry name" value="CellCycle_DNARepair_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031906">
        <property type="entry name" value="RTT107_BRCT_6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47667">
        <property type="entry name" value="REGULATOR OF TY1 TRANSPOSITION PROTEIN 107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47667:SF1">
        <property type="entry name" value="REGULATOR OF TY1 TRANSPOSITION PROTEIN 107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12738">
        <property type="entry name" value="PTCB-BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16770">
        <property type="entry name" value="RTT107_BRCT_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16771">
        <property type="entry name" value="RTT107_BRCT_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRCT" evidence="2">
        <location>
            <begin position="3"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="2">
        <location>
            <begin position="115"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="2">
        <location>
            <begin position="336"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="522"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="602"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="528"/>
            <end position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR21860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="913" mass="102394" checksum="0ABB16425EC0B894" modified="2009-07-28" version="1">MSASSKLFQGLNFVIVANKETEPETERIIEILENNSANKCTIYNVLENEAHQALDKDVFCKTFDFEVHAIIASTCDFWFYRLAAFDFLIPVVTSDWVNACVATRRITRASSFSPDSSHLLKNHQIYVSKHAFSPSEYTLYSAIVRALGGVCIDHLSSKTSHILTNDAQDPAIAAVADIKSLSIKYVLPTWAVAVFASGDLVSETPYSLDPHDRPEDVEDKSASTWELVQKTQVGSCTDFLCGHEFFFSSDLILPSQCFDFLVDFILAAGGNAIRHSEPMEISKFNGDCFLGQNSISEDFISANSRGLHKGNLSWLFHMWSLQSFVLPQFKLLLSPLRPPVFNKGQLILSYTNYLGQQRYYIQKLVEALGGVSTTELTKKNTHLLTCLPLGQKYEAALRWEGTCKIANHLWLEDCYRKQTQVPFVGTRYNEIPAPGGLSTKLGQMPLEINDEDLTDTILPQEADMPSQNHILLRGQDSATETPTQCEDVLPDNGIFPDPVTIPEEVPTPEEPHPQMQINIVEGTSSRQEPLEKHTEHKVSSKRAFDEFLKDDAFSPDQTLEPHKDNTPSSNIAAEGAPDIVGPQRDDLSQEATSYVPLRQASPNASFSSLPHDHSQFLPSSNSRKAKEKAALKLHTDMEALNEFEQNLKRKKNRSLLPEEIQKLNKLKEVGEKVREHLETVAGRPKGKSMKPYDIIAVCTGCHEEIDEFDLELLRRVGVTILKAVDVQCNTIIAPKKMRTAKFLTSFGFHPLKWALMPEFVSELLSIFRQTTPIQELPKPEDYLIPEIEPEVLKKTKLSTKVFQRAGIKSINLTEGVPGGEEVLSSILKAHGVLNVHMLPKKYTEEDVVINNGKKNSPTHILIAQKASQSKKFAKLCRNAGENKVMVVEWNWCVESIFTLEANLETSRYVVYRS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-11-24" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDM1</accession>
    <name>RSSA_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Small ribosomal subunit protein uS2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">40S ribosomal protein S0</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">RPS0</name>
        <name type="ordered locus">KLTH0C02112g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains about 33 different proteins and 1 molecule of RNA (18S). The 60S subunit contains about 49 different proteins and 3 molecules of RNA (25S, 5.8S and 5S). Interacts with RPS21.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDM1"/>
    <dbReference type="SMR" id="C5DDM1"/>
    <dbReference type="FunCoup" id="C5DDM1">
        <property type="interactions" value="1456"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDM1"/>
    <dbReference type="KEGG" id="lth:KLTH0C02112g"/>
    <dbReference type="eggNOG" id="KOG0830">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058171_2_0_1"/>
    <dbReference type="InParanoid" id="C5DDM1"/>
    <dbReference type="OMA" id="VKNFFEP"/>
    <dbReference type="OrthoDB" id="414863at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000461">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01425">
        <property type="entry name" value="RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10490:FF:000010">
        <property type="entry name" value="40S ribosomal protein S0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03015">
        <property type="entry name" value="Ribosomal_S2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001865">
        <property type="entry name" value="Ribosomal_uS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018130">
        <property type="entry name" value="Ribosomal_uS2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027498">
        <property type="entry name" value="Ribosomal_uS2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005707">
        <property type="entry name" value="Ribosomal_uS2_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023591">
        <property type="entry name" value="Ribosomal_uS2_flav_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01012">
        <property type="entry name" value="uS2_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11489">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN SA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00318">
        <property type="entry name" value="Ribosomal_S2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00395">
        <property type="entry name" value="RIBOSOMALS2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52313">
        <property type="entry name" value="Ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00962">
        <property type="entry name" value="RIBOSOMAL_S2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00963">
        <property type="entry name" value="RIBOSOMAL_S2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0007">Acetylation</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="initiator methionine" description="Removed" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000389277" description="Small ribosomal subunit protein uS2">
        <location>
            <begin position="2"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="213"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="222"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="modified residue" description="N-acetylserine" evidence="1">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="251" mass="27727" checksum="CF334A4722AECA2F" modified="2009-07-28" version="1">MSLPATFDLTPEDAQLLLAANVHLGSKNVQVHQEPYVFKTRPDGVNVVNVGKTWEKIVLAARIIAAIPNPEDVVAISSRTYGQRAVLKFSAHTGATAIAGRFTPGSFTNYITRSFKEPRLVIVTDPRSDAQAIKESSYVNIPVIALTDLDSPSEYVDVAIPCNNKGKHSIGLIWYLLAREVLRLRGSLVDRTQPWSIMPDLYFYRDPEEVDQQVAEEATAAADEDVKEEVAEEQTEAADWAEGNTEEVASW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDP0</accession>
    <name>C5DDP0_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA-directed RNA polymerase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0C02552g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 12">Belongs to the archaeal rpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552339.1">
        <property type="nucleotide sequence ID" value="XM_002552293.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDP0"/>
    <dbReference type="FunCoup" id="C5DDP0">
        <property type="interactions" value="748"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDP0"/>
    <dbReference type="GeneID" id="8291199"/>
    <dbReference type="KEGG" id="lth:KLTH0C02552g"/>
    <dbReference type="eggNOG" id="KOG2907">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093932_1_1_1"/>
    <dbReference type="InParanoid" id="C5DDP0"/>
    <dbReference type="OMA" id="EMQYHTL"/>
    <dbReference type="OrthoDB" id="10056816at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055029">
        <property type="term" value="C:nuclear DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006363">
        <property type="term" value="P:termination of RNA polymerase I transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10507">
        <property type="entry name" value="Zn-ribbon_RPA12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.10:FF:000019">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019761">
        <property type="entry name" value="DNA-dir_RNA_pol-M_15_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012164">
        <property type="entry name" value="Rpa12/Rpb9/Rpc10/TFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034004">
        <property type="entry name" value="Zn_ribbon_RPA12_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001529">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001222">
        <property type="entry name" value="Znf_TFIIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239:SF14">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02150">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01096">
        <property type="entry name" value="Zn_ribbon_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005586">
        <property type="entry name" value="RNApol_RpoM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00661">
        <property type="entry name" value="RPOL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00440">
        <property type="entry name" value="ZnF_C2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01030">
        <property type="entry name" value="RNA_POL_M_15KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00466">
        <property type="entry name" value="ZF_TFIIS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51133">
        <property type="entry name" value="ZF_TFIIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8 12" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="3 9 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIS-type" evidence="13">
        <location>
            <begin position="82"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="10">
        <location>
            <begin position="10"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR005586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR005586-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR005586-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR21901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="125" mass="13600" checksum="D9A5866916236E25" modified="2009-07-28" version="1">MSVVGSLIFCAECGNLLDNPSAVEGAQLECAQCGAEYAKAKFSNLRVVTSTADDAFPSALRSKKSVVKTSLKKDELEEGATIREKCPQCGNDEMHYHTLQLRSADEGATVFYTCTSCGYRFRTNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDW2</accession>
    <name>C5DDW2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0C04268p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0C04268g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="CAR21973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002552365.1"/>
    </dbReference>
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        <property type="interactions" value="151"/>
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    <dbReference type="InParanoid" id="C5DDW2"/>
    <dbReference type="OMA" id="AYIDMAS"/>
    <dbReference type="OrthoDB" id="360390at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12296">
        <property type="entry name" value="RRM1_Prp24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12298">
        <property type="entry name" value="RRM3_Prp24"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RRM4_Prp24"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="Euk_RNA-bind_prot"/>
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        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034397">
        <property type="entry name" value="Prp24_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034540">
        <property type="entry name" value="Prp24_RRM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
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        <property type="entry name" value="RRM_dom"/>
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        <property type="entry name" value="RRM_occluded"/>
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        <property type="entry name" value="OS02G0815200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025:SF1">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16842">
        <property type="entry name" value="RRM_occluded"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="RNA-binding domain, RBD"/>
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    </dbReference>
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        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="41"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="117"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="210"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="406"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="25"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="415"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR21973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="450" mass="50784" checksum="E9264086A67CD773" modified="2009-07-28" version="1">MSADELTEPQLKRRLSDASNTDETSTEKKPRAEPKKYREYTTVIVRNLPQSYNFHKVRKLFNSCGKVGHVDVHTSTDGQCKVARIEFENYSDVLSALTKSGKVLGGNEIEVEELSKSTLWATNFPPTYDQGKLMQLFGQFGGTVLSVRLPSLRFDSRRRFAYIDMASKQEASLALEKLDGVEIENYKMIVKLSQPSNAAARTDKAVIERRQVLVRNLNYNTNEAALSAIFAKFGHVEQINLPRTRENKHGEIDTKGETKERLNEGFAFITFSDASCARSSLSLNGTEIDNRILDVTLADRRPYLERQKVKHIMASKNQDGSVLGIYPLSDKIPTPQIRCFIVEQAKVKKEDIKKIYLVPDHEGALVVARSGPIAAKISLSIHGSLFNKRTIMCCSPKELQRQVHARYGRSSTKQRTHEDSKDIEITRSAPPLEGEKKMNNEDFRKLFLGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDY5</accession>
    <name>C5DDY5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0C04818p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0C04818g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="5">Protein modification.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552434.1">
        <property type="nucleotide sequence ID" value="XM_002552388.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDY5"/>
    <dbReference type="FunCoup" id="C5DDY5">
        <property type="interactions" value="302"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDY5"/>
    <dbReference type="GeneID" id="8291301"/>
    <dbReference type="KEGG" id="lth:KLTH0C04818g"/>
    <dbReference type="eggNOG" id="KOG0425">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030988_1_1_1"/>
    <dbReference type="InParanoid" id="C5DDY5"/>
    <dbReference type="OMA" id="FQAHIKF"/>
    <dbReference type="OrthoDB" id="19692at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23811">
        <property type="entry name" value="UBCc_ScCDC34-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000063">
        <property type="entry name" value="CDC34p Ubiquitin-conjugating enzyme (E2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="9">
        <location>
            <begin position="7"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="185"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="190"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="221"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="250"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00043952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR21996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="275" mass="31639" checksum="9A8233279AF4A873" modified="2009-07-28" version="1">MSNRKNTAASLLLRQYRELTDAKKAIPSFHIELDDDSNIFLWNIGVMVLNEDSIYHGGYFKAQMRFPEDFPFSPPTFRFTPAIYHPNVYRDGRLCISILHTSGDPTSEEPDSETWSPVQTVESVLISIVSLLEDPNISSPANVDAAVDYRKNIEQYKQRVQLEVERSKQDIPEGFVMPTSRTAYVAQRQAEPEETRDLGDNFWYDSDDEDDADIYGYDHNEEQEDNDIQFDDSENENDDDDDVSVDNDSVMDRKQPNKAEDESDDLEDGELKLEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEE3</accession>
    <name>C5DEE3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphatidylglycerol/phosphatidylinositol transfer protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0C08492g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the intermembrane transfer of phosphatidylglycerol and phosphatidylinositol.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NPC2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552592.1">
        <property type="nucleotide sequence ID" value="XM_002552546.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEE3"/>
    <dbReference type="FunCoup" id="C5DEE3">
        <property type="interactions" value="125"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEE3"/>
    <dbReference type="GeneID" id="8291466"/>
    <dbReference type="KEGG" id="lth:KLTH0C08492g"/>
    <dbReference type="eggNOG" id="KOG4680">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097982_0_0_1"/>
    <dbReference type="InParanoid" id="C5DEE3"/>
    <dbReference type="OMA" id="HQTYDLC"/>
    <dbReference type="OrthoDB" id="6409159at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032366">
        <property type="term" value="P:intracellular sterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00917">
        <property type="entry name" value="PG-PI_TP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.220.10">
        <property type="entry name" value="Ganglioside GM2 activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036846">
        <property type="entry name" value="GM2-AP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003172">
        <property type="entry name" value="ML_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033917">
        <property type="entry name" value="ML_PG-PI_TP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039670">
        <property type="entry name" value="NPC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11306">
        <property type="entry name" value="NIEMANN PICK TYPE C2 PROTEIN NPC2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11306:SF0">
        <property type="entry name" value="PHOSPHATIDYLGLYCEROL_PHOSPHATIDYLINOSITOL TRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02221">
        <property type="entry name" value="E1_DerP2_DerF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00737">
        <property type="entry name" value="ML"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002947807" description="Phosphatidylglycerol/phosphatidylinositol transfer protein" evidence="8">
        <location>
            <begin position="20"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="MD-2-related lipid-recognition" evidence="9">
        <location>
            <begin position="45"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR22154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="174" mass="18985" checksum="9507E7CA82A514B5" modified="2009-07-28" version="1">MTRTLQLYLAALLLALAAASPVFPAFFGLPGTRSQNKPIPGDSPLEQCDADDKQLLTIQLVNLLPNPPVRGENVTVSAAGHVKETIKEGAYVDVEVRLGYIKLLTQTYDLCEELEKNDVGGLKCPIAPGDYKIDKTVEIPQEVPPGKYTVLARAYTEDDDEITCLSGDIFFPSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEE5</accession>
    <name>C5DEE5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Small ribosomal subunit protein mS37</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0C08536g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the mitochondrial small ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mS37 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552594.1">
        <property type="nucleotide sequence ID" value="XM_002552548.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEE5"/>
    <dbReference type="FunCoup" id="C5DEE5">
        <property type="interactions" value="126"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEE5"/>
    <dbReference type="GeneID" id="8291468"/>
    <dbReference type="KEGG" id="lth:KLTH0C08536g"/>
    <dbReference type="eggNOG" id="ENOG502SBQ7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_162186_0_0_1"/>
    <dbReference type="InParanoid" id="C5DEE5"/>
    <dbReference type="OMA" id="INYHAAR"/>
    <dbReference type="OrthoDB" id="2210at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010625">
        <property type="entry name" value="CHCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017264">
        <property type="entry name" value="Ribosomal_mS37_fun"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28066">
        <property type="entry name" value="37S RIBOSOMAL PROTEIN MRP10, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28066:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06747">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037706">
        <property type="entry name" value="MRP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="CHCH" evidence="3">
        <location>
            <begin position="30"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR037706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR22156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="95" mass="10525" checksum="37DC5CBD9D2DA32D" modified="2009-07-28" version="1">MSGKVPVYRLPPLPRLKVKKPIVQQEANKCLVLMSNLLQCWSSNGHMNPVCENLVKDLKACSSESAMGKNSGVQKSNINYHAARLYDRVSGKPND</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEP1</accession>
    <name>C5DEP1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0C10846p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0C10846g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552690.1">
        <property type="nucleotide sequence ID" value="XM_002552644.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEP1"/>
    <dbReference type="FunCoup" id="C5DEP1">
        <property type="interactions" value="254"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEP1"/>
    <dbReference type="GeneID" id="8291572"/>
    <dbReference type="KEGG" id="lth:KLTH0C10846g"/>
    <dbReference type="eggNOG" id="KOG4090">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093520_0_1_1"/>
    <dbReference type="InParanoid" id="C5DEP1"/>
    <dbReference type="OMA" id="CDADARN"/>
    <dbReference type="OrthoDB" id="1106148at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055304">
        <property type="entry name" value="CHCHD2/10-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13523:SF2">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN CONTAINING 2, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13523">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN CONTAINING 2/NUR77"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="24"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="88"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="153" mass="15968" checksum="DCC6CB85E732B7E1" modified="2009-07-28" version="1">MARSRGGVRSRPAPAQTRSASSMAAYPPAQQHAPQTSAYSHPQSATQPRQPGLFAQMASTAAGVAVGSAVGHTVGAGITGMFSGSSSAPAEQQQQQLAAAAPAQQGFAQEQARSCDLDARNLTRCLEENSGNMQICEFYLQQLKACQEAARPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DF28</accession>
    <name>C5DF28_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0D11748p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0D11748g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553221.1">
        <property type="nucleotide sequence ID" value="XM_002553175.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DF28"/>
    <dbReference type="FunCoup" id="C5DF28">
        <property type="interactions" value="69"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DF28"/>
    <dbReference type="GeneID" id="8295461"/>
    <dbReference type="KEGG" id="lth:KLTH0D11748g"/>
    <dbReference type="eggNOG" id="KOG3138">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_5_3_1"/>
    <dbReference type="InParanoid" id="C5DF28"/>
    <dbReference type="OMA" id="EYAGAIC"/>
    <dbReference type="OrthoDB" id="47374at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031415">
        <property type="term" value="C:NatA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051556">
        <property type="entry name" value="N-term/lysine_N-AcTrnsfr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42919">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42919:SF8">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR22783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="162" mass="18195" checksum="A492CC22698A90C8" modified="2009-07-28" version="1">MSRNLVNLDNVYSNNVGTFQKIANTVLNVTYPDKFFQEQFDGGKETLFAQLGYYGEVAVGAVRAQLISNKNGGVKASGVYIQIFAVLENYRGKGVGSQLLKFVEEQCKAHFQHNIYVHVAVDNEKGIQWYQRKGFEQEGEVLKNYYKDTTGAPDALILKKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>C5DF44</accession>
    <name>C5DF44_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0D12100p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0D12100g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553237.1">
        <property type="nucleotide sequence ID" value="XM_002553191.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DF44"/>
    <dbReference type="FunCoup" id="C5DF44">
        <property type="interactions" value="53"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DF44"/>
    <dbReference type="GeneID" id="8295477"/>
    <dbReference type="KEGG" id="lth:KLTH0D12100g"/>
    <dbReference type="eggNOG" id="ENOG502S2PC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052222_0_0_1"/>
    <dbReference type="InParanoid" id="C5DF44"/>
    <dbReference type="OMA" id="ICASKQI"/>
    <dbReference type="OrthoDB" id="4036231at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="255"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="311"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="109"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="405" mass="44122" checksum="6B22D1338A098CDF" modified="2009-07-28" version="1">MPRKFLGQRLSAGTDFVRPASLNLTELDLDEIPDVPQGTSLDNRELGSYPAEDVRCRRLSRPFGGTLRLKKRLSSVPELLLRDLGRLEQKTGVKLAQNSNTTSERPARRSAPPCSAPRWTKDQRGKNLVTAAKRRPTSSTSNHQSYVPSPLAGPPSMGRGQTLETFPVTAVQIQFTPERPSHNRQKSDGELLFEEIIKAYANAPEREGPEPLALKAEMQSSLGKHFVHDSASRTFGQVKKQPLGFFGTVVKEEELSGIEGPKHTRRLSPPSLPVALDATLPGVEYSGSSSSDPSSSGEEFSDLGSILESLKASPRGARSSDEEENYFSADDLLSSPTKVNLPTARSARCSLRQVRTSQVTPVVLDFDELVSASEDEGEGSDAISQLQEELDDLGIMDTSSSIYDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>C5DF80</accession>
    <name>C5DF80_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein mL53</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">54S ribosomal protein L44, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0D12914g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mL53 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553273.1">
        <property type="nucleotide sequence ID" value="XM_002553227.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DF80"/>
    <dbReference type="FunCoup" id="C5DF80">
        <property type="interactions" value="121"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DF80"/>
    <dbReference type="GeneID" id="8295513"/>
    <dbReference type="KEGG" id="lth:KLTH0D12914g"/>
    <dbReference type="eggNOG" id="ENOG502S452">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131037_1_0_1"/>
    <dbReference type="InParanoid" id="C5DF80"/>
    <dbReference type="OMA" id="MDFNCSK"/>
    <dbReference type="OrthoDB" id="4136894at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000260">
        <property type="entry name" value="Mitochondrial ribosomal protein L44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019716">
        <property type="entry name" value="Ribosomal_mL53"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042776">
        <property type="entry name" value="Ribosomal_mL53_fung"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28236">
        <property type="entry name" value="54S RIBOSOMAL PROTEIN L44, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28236:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10780">
        <property type="entry name" value="MRP_L53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00077936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR22835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="98" mass="11066" checksum="9855CAFAD89F5F98" modified="2009-07-28" version="1">MITKFFTKVSVRFNPFGKEAKSARLFLSAIPPAQRLSGTSIQNELLTGGSVKTPIVKVTFKDKTEMEVDPSNLTFKELGNYFDRYSRKLSLKESIESQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DF91</accession>
    <name>C5DF91_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0D13200p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0D13200g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peroxisomal membrane protein PXMP2/4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553284.1">
        <property type="nucleotide sequence ID" value="XM_002553238.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DF91"/>
    <dbReference type="FunCoup" id="C5DF91">
        <property type="interactions" value="34"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DF91"/>
    <dbReference type="GeneID" id="8295526"/>
    <dbReference type="KEGG" id="lth:KLTH0D13200g"/>
    <dbReference type="eggNOG" id="KOG1944">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049109_8_0_1"/>
    <dbReference type="InParanoid" id="C5DF91"/>
    <dbReference type="OMA" id="FMCWGFI"/>
    <dbReference type="OrthoDB" id="10267969at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007248">
        <property type="entry name" value="Mpv17_PMP22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266:SF50">
        <property type="entry name" value="VACUOLAR MEMBRANE PROTEIN YOR292C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04117">
        <property type="entry name" value="Mpv17_PMP22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="55"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="187"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="233"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="263"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR22846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="319" mass="36014" checksum="97D4BB424E5F4ACE" modified="2009-07-28" version="1">MPIELLGRDQIAVSYGEDEEEPVWARQRPCDWRSRAMRWAAERARHARKAVETCTILRVGIVHWVMLAVWAALLLKIASVYGRWYARSALLTTICTNALLFGTSDILAQCVLQYTWAPVDPTPAPLDATARALAVRYALFTDQESDTDSASIFNEYGPGASSSEVASGAVAPSPPLGQPLCPPLFDFYRWLCFMAWGSFLSFFQVPWYRILNYLYTEDPTVVQVLERVLSDQLVYSPISLYCFFMYANYIMQKGDAASFRAKIQRLYIGTLGCNYLLWPAVQFINFLAVPKHLQVPFSSSVGVLWNCFLSMRNASHPAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFB8</accession>
    <name>C5DFB8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0D13816p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0D13816g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553311.1">
        <property type="nucleotide sequence ID" value="XM_002553265.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFB8"/>
    <dbReference type="FunCoup" id="C5DFB8">
        <property type="interactions" value="119"/>
    </dbReference>
    <dbReference type="GeneID" id="8295553"/>
    <dbReference type="KEGG" id="lth:KLTH0D13816g"/>
    <dbReference type="eggNOG" id="ENOG502SFCR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125609_1_0_1"/>
    <dbReference type="InParanoid" id="C5DFB8"/>
    <dbReference type="OMA" id="KWKSKRY"/>
    <dbReference type="OrthoDB" id="4036571at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="31"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="153" mass="15920" checksum="2A7E5C6D7ED209AA" modified="2009-07-28" version="1">MSTATHKPRLTGWAAAAAKAAPKTQSKPEESPSSSSSAAAARSTAAASSKSSTGTKTRPPAAARGVSPPASAPASKDPTKKNKKTSRPAFNSEQVAQFLRSQFAAQSSQPNTTVYTKPKTHSSHGSPDWGTTTSKWKTKKYGCLSDVARSLRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFF8</accession>
    <name>C5DFF8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0D14740p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0D14740g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553351.1">
        <property type="nucleotide sequence ID" value="XM_002553305.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFF8"/>
    <dbReference type="FunCoup" id="C5DFF8">
        <property type="interactions" value="14"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFF8"/>
    <dbReference type="GeneID" id="8295595"/>
    <dbReference type="KEGG" id="lth:KLTH0D14740g"/>
    <dbReference type="eggNOG" id="ENOG502S0BA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061225_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFF8"/>
    <dbReference type="OMA" id="FLQWSSY"/>
    <dbReference type="OrthoDB" id="4053690at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="CDD" id="cd23994">
        <property type="entry name" value="Seipin_Sei1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="240"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="277" mass="31750" checksum="F1EEE2A4B4E81B2D" modified="2009-07-28" version="1">MQINLTLPLKWAQWWAYILVLMLVNIAFIFPLSAFLFRDFYSRMIPPDTTRTVSFSESKREMGGWTGKTTFQFDLKRYSTEDAKLPFVSPNGFAQSVPLRSDIPYNMDVNLNIFCLNKVTDWNIRDAEVSISVLKSGKSNAAVVFRKTLLLSCANTRDVHSVSGTRRLTTTFAKQIQDELVNSYSLENPFFVEHDVKCLEVSLRCAGNANLIIDPNSSELKLSMNFENSLRNLMIRWKKLTYAVGTVVFDVIITSFFFLAFGLTFLRAGRVKERKDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFS8</accession>
    <name>C5DFS8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Mitochondrial import inner membrane translocase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0D17600g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion inner membrane</location>
            <topology evidence="7">Peripheral membrane protein</topology>
            <orientation evidence="7">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the small Tim family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR23033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553471.1">
        <property type="nucleotide sequence ID" value="XM_002553425.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFS8"/>
    <dbReference type="FunCoup" id="C5DFS8">
        <property type="interactions" value="559"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFS8"/>
    <dbReference type="GeneID" id="8295721"/>
    <dbReference type="KEGG" id="lth:KLTH0D17600g"/>
    <dbReference type="eggNOG" id="KOG3489">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_141397_1_0_1"/>
    <dbReference type="InParanoid" id="C5DFS8"/>
    <dbReference type="OMA" id="NEICWDK"/>
    <dbReference type="OrthoDB" id="344165at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.810">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit tim13 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004217">
        <property type="entry name" value="Tim10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035427">
        <property type="entry name" value="Tim10-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02953">
        <property type="entry name" value="zf-Tim10_DDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144122">
        <property type="entry name" value="Tim10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2 7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="3 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0811">Translocation</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Tim10-like" evidence="8">
        <location>
            <begin position="23"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR23033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="87" mass="9839" checksum="D6BE013FB9F0B934" modified="2009-07-28" version="1">MSSITQNELASLDESSKKEIMTFLESENSKQKVQMSIHQFTNICFKQCATTMNSGNLSSQEETCLNNCVNRFLDTNIRIVKGLQSIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFS9</accession>
    <name>C5DFS9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0D17622p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0D17622g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR23034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553472.1">
        <property type="nucleotide sequence ID" value="XM_002553426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFS9"/>
    <dbReference type="FunCoup" id="C5DFS9">
        <property type="interactions" value="66"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFS9"/>
    <dbReference type="GeneID" id="8295722"/>
    <dbReference type="KEGG" id="lth:KLTH0D17622g"/>
    <dbReference type="eggNOG" id="ENOG502S4RM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105159_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFS9"/>
    <dbReference type="OMA" id="MFFPERS"/>
    <dbReference type="OrthoDB" id="4035795at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR23034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="225" mass="25857" checksum="5853D3F047F272EE" modified="2009-07-28" version="1">MDNDNQLSIYVGSVEREIENKGYFLKQVLDAIEVLKLKRHADTEESHGSWHEFSRKVMFTSERSDPIGLCLAFCEERLRLKTSKEYLTMEPLDALKEALKFQKSLNRGLESFMELMKVRNKGSGPVFDQETGDLTKHQNSVLWRKLQIFVENFLAIDTSSESTEADIVAGKIFEVLSHLIKGQKAVQKDCVQQQLPGFYRLLIKTKCTYTDNEGCVRLIFHESAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFY1</accession>
    <name>C5DFY1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">KLTH0D00858p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0D00858g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transient receptor potential (TRP) ion channel family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552761.1">
        <property type="nucleotide sequence ID" value="XM_002552715.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFY1"/>
    <dbReference type="FunCoup" id="C5DFY1">
        <property type="interactions" value="74"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFY1"/>
    <dbReference type="GeneID" id="8294981"/>
    <dbReference type="KEGG" id="lth:KLTH0D00858g"/>
    <dbReference type="eggNOG" id="ENOG502QSVZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010226_0_1_1"/>
    <dbReference type="InParanoid" id="C5DFY1"/>
    <dbReference type="OMA" id="FQAQAFI"/>
    <dbReference type="OrthoDB" id="5212126at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009272">
        <property type="term" value="P:fungal-type cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010308">
        <property type="entry name" value="TRP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040241">
        <property type="entry name" value="TRP_Flc/Pkd2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032800">
        <property type="entry name" value="TRP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31145:SF2">
        <property type="entry name" value="FLAVIN CARRIER PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31145">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_7G01610)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06011">
        <property type="entry name" value="TRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14558">
        <property type="entry name" value="TRP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01320">
        <property type="entry name" value="TRP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950465" evidence="9">
        <location>
            <begin position="29"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="360"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="415"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="447"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="504"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="531"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="561"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="595"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="domain" description="ML-like" evidence="10">
        <location>
            <begin position="33"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="698"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="698"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="751"/>
            <end position="775"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR22323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="775" mass="86322" checksum="B3B5514ADC2CA1EC" modified="2009-07-28" version="1">MRPALTGPMRVLFLVFVFLQFWVSPASASNTSRHLETTSLLTCMQNSQLTASFFEVKFYPSLNKVYFNIDATTTISTNITAEVQVIVYGLNLMERSINFCNLDQPALCPLQAGRIDVNSSFTVDSDITSNIPNVAYTVPDLDAQVRVVVYSTSDTDHGTPLACVVAPLGNGKTVQTKYAAWPIAAISGLGVLTSGFVSVIGHSTTAAHIASNSISLFIYFQNLAITSMMGVSRVPPIAAAWSQNFQWSMGIINVKFMQDIFNWYIKATGGEPTVVVANKDVLSIAVQKKRVKRALVEGAKHLYKRLTISTTDSMDLDSFLSEQGLYTGNERNSTNYASKTLVLHGIQRVAYKAGIELSNLFLTGVVFTFFFVFIVVIALMFFKALVELLTRTRVMAESSKFFEYRKSWGNIIKGTLFRLSIIAFPQLSILCIWEFTQRDSAATMVDAIVILITIGAVLLYGVIKVVLRGRESARLYKTPAYMLYGDSNFLNRYGFLYVQFKADLYWWLMPLLGYSFLRSLFVAVLQNNGKAQAMVTFIIEMVYFVAICWKRPYLDKRTNAFNIVIHLVNFFNALFFLFFSGLFRQPQIVSSVMAVVLFVMNAVFALFLLIFTIVTCSLALIYRNPDARYQPMKDDRVSFIPKIQTNGNASKSETELFELGKAVMTTNEASNISSDKSYRNQSSKKLLFDDEVDDSSIFDDHSGAREKRANSGEAVQPSSAVLGPSTFGTYQKIPTAPSSRGAQNLRAPENTFGNTSYQGYTGASSNPYSKNNPYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>C5DG05</accession>
    <name>C5DG05_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0D01408p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0D01408g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552785.1">
        <property type="nucleotide sequence ID" value="XM_002552739.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DG05"/>
    <dbReference type="FunCoup" id="C5DG05">
        <property type="interactions" value="15"/>
    </dbReference>
    <dbReference type="GeneID" id="8295005"/>
    <dbReference type="KEGG" id="lth:KLTH0D01408g"/>
    <dbReference type="eggNOG" id="ENOG502S10A">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029218_0_0_1"/>
    <dbReference type="InParanoid" id="C5DG05"/>
    <dbReference type="OMA" id="CLAKFTL"/>
    <dbReference type="OrthoDB" id="4041451at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000372">
        <property type="term" value="P:Group I intron splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025694">
        <property type="entry name" value="MNE1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13762">
        <property type="entry name" value="MNE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR22347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="640" mass="72091" checksum="F0822A739A025A12" modified="2009-07-28" version="1">MLKKKGAPLSLQNFAAKILGGDKLVKQLENGSLKTASTKALSRSDVWLREALVNTATTRRSYTLPHSGAIYPSRSIDVSLNVILSTLRRLRFANDAGGYFILLNRIKSSRVRWVSSSGKPIAGNVPVELYREVSHMLRAKSSGFQREELVCLAKFTLKLLRDYNEIVAFRRPLKPDKIFLRNCADIVTRLGSMTFLNAFNNLIEHEDIKVYAMISFYLQTKQIAHLVQFLESSNLGRDSLGLELASSLLLHCIREFVTLGLEDIACSTLELLLKKCALDLEKPLNDVKLIAEKFGAFKVQLAINKANDPELRSAFVPWKTFQKDLSFKDYINLFDESEIDFYNETASMEFLSSKLPHHRMALEGWYHLFEETRPHPTASASLKAFHFNTILSTVASTGSLGLVTLFWRHSIVKLGLTEDFLNSHNLISSRNCGGFHVILRSVGKSSSAKLAGYQLFNFLKHDFKKLTSNHGVATFNLTNKDYFYLMQSATRGPNVSSVYFYLFHHLLDAGDTCFSFGKDGSPSWQPGEPILSLIKSKFSKDWHLTGVGKIVERVGTWYKNEKDRGVYSSHIQKDVLVDIFGPHFIQELSPKTLLQLESDSNNAMDAKSSSVYSLIADCENTERIKETLGHLTQSIKGKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGB3</accession>
    <name>C5DGB3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0D03916p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0D03916g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552893.1">
        <property type="nucleotide sequence ID" value="XM_002552847.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGB3"/>
    <dbReference type="FunCoup" id="C5DGB3">
        <property type="interactions" value="477"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGB3"/>
    <dbReference type="GeneID" id="8295118"/>
    <dbReference type="KEGG" id="lth:KLTH0D03916g"/>
    <dbReference type="eggNOG" id="KOG4210">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026791_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGB3"/>
    <dbReference type="OMA" id="EIRIVMN"/>
    <dbReference type="OrthoDB" id="439808at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12447">
        <property type="entry name" value="RRM1_gar2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000550">
        <property type="entry name" value="Nuclear localization sequence binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051106">
        <property type="entry name" value="RNA-bind/splicing_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48028">
        <property type="entry name" value="GLYCINE-RICH RNA-BINDING PROTEIN RZ1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48028:SF4">
        <property type="entry name" value="SC35-LIKE SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="256"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="357"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="324"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="426"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="11"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="28"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="80"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="129"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="157"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="175"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="217"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="232"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="242"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="443"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR22455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="505" mass="53692" checksum="770BB0F3624FBB52" modified="2009-07-28" version="1">MAKQVAKKVSKKDVKSKKQEKEAVAAKSESSSSSSSSESSSSSSESSSSSSESESESSSSSSSSDSESSDSSSDSEEEQEVKKDTKKDTKKDTKKEAKKDSKKDSKKEAKKETVKEAKKGSKKAVAPESDSESGSESDTESSDSESSDSDSSSSSESESEAEAEAETESKKAKESATSSSSSNSSSGSDSSSSSSSSESEGSSSSSDSDSSSEEEEAKEKDTSSKKRKAEESDVSEESEDATESKKAKTESNGEPATIFVGRLSWSVDDEWLKTEFEPIGGVISARVIMERGTDRSRGYGYVDFENKFYAEKAVKEMHGKEIDGRPINCDMSTSKPAGNPRNDRAKQFGDTPSQPSDTLFLGNLSFNADRDNIFETFAEHGEVVSVRLPTHPETNQPKGFGYVQYSSVDEAQKAFEALQGHYIDNRPVRLDFSTPKPRDEGSRGGFRGGRGGSRGGSRGGFGGGRGGFGGGNRGDFKPSGSGSNSAPLGKGRQTAEFQGNKKKFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGI2</accession>
    <name>C5DGI2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">glutamine--fructose-6-phosphate transaminase (isomerizing)</fullName>
            <ecNumber evidence="4">2.6.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">D-fructose-6-phosphate amidotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Hexosephosphate aminotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0D05522g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Involved in amino sugar synthesis (formation of chitin, supplies the amino sugars of asparagine-linked oligosaccharides of glycoproteins).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-fructose 6-phosphate + L-glutamine = D-glucosamine 6-phosphate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:13237"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="EC" id="2.6.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; alpha-D-glucosamine 6-phosphate from D-fructose 6-phosphate: step 1/1.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.16" evidence="4"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552962.1">
        <property type="nucleotide sequence ID" value="XM_002552916.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGI2"/>
    <dbReference type="FunCoup" id="C5DGI2">
        <property type="interactions" value="1170"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGI2"/>
    <dbReference type="GeneID" id="8295190"/>
    <dbReference type="KEGG" id="lth:KLTH0D05522g"/>
    <dbReference type="eggNOG" id="KOG1268">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012520_5_0_1"/>
    <dbReference type="InParanoid" id="C5DGI2"/>
    <dbReference type="OMA" id="ASEYRYA"/>
    <dbReference type="OrthoDB" id="15235at2759"/>
    <dbReference type="UniPathway" id="UPA00113">
        <property type="reaction ID" value="UER00528"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004360">
        <property type="term" value="F:glutamine-fructose-6-phosphate transaminase (isomerizing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006031">
        <property type="term" value="P:chitin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006048">
        <property type="term" value="P:UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00714">
        <property type="entry name" value="GFAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05008">
        <property type="entry name" value="SIS_GlmS_GlmD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05009">
        <property type="entry name" value="SIS_GlmS_GlmD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10490:FF:000001">
        <property type="entry name" value="Glutamine--fructose-6-phosphate aminotransferase [isomerizing]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10490:FF:000002">
        <property type="entry name" value="Glutamine--fructose-6-phosphate aminotransferase [isomerizing]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047084">
        <property type="entry name" value="GFAT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035466">
        <property type="entry name" value="GlmS/AgaS_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035490">
        <property type="entry name" value="GlmS/FrlB_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001484">
        <property type="entry name" value="PRK00331.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10937">
        <property type="entry name" value="GLUCOSAMINE--FRUCTOSE-6-PHOSPHATE AMINOTRANSFERASE, ISOMERIZING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10937:SF0">
        <property type="entry name" value="GLUTAMINE--FRUCTOSE-6-PHOSPHATE TRANSAMINASE (ISOMERIZING)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13522">
        <property type="entry name" value="GATase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SIS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="8" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="11">
        <location>
            <begin position="2"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="12">
        <location>
            <begin position="374"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="12">
        <location>
            <begin position="546"/>
            <end position="691"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR22524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="701" mass="78349" checksum="40EA9E15CAD76A2B" modified="2009-07-28" version="1">MCGIFGYCNYLVEKSRGDVIDTLVEGLQRLEYRGYDSTGIAIDGDEPDSTLIYKQIGKVAALEKEIEAEKPNRDVTFVSHCGIAHTRWATHGEPKWSNCHPQRSDTASDFVIVHNGIITNFRELKQLLINKGYKFESDTDTECIAKLFKHLYDTNLKHGNEPDFHELTMQVLLELDGSYGLLCRSVHYPNEVIATRKGSPLLIGVKSERKLKVDFVDVEFPDTDEAQPEIPLNSTRGEARNFLPIASNESALRRSQSRAFLSEDGAPNPVEFFVSSDAASVVKHTKKVLFLEDDDIAHIYDGELHIHRSRREIGASATRSIQTLEMELAQIMKGSYKHFMQKEIFEQPDSTFNTMRGRIDFENNSVMLGGLNSWLPVIRRARRLIMIACGTSYHSCLATRAIFEELSEIPVSVELASDFLDRKTPVFRDDIAVFVSQSGETADTMLALKYCLERGALTVGIVNNVGSSISRVTHCGVHINAGPEIGVASTKAYTSQYIALVMFALSLSDDRVSKLDRRREIIQGLKKIPEQIRKVLLMEPRIKELCEKELKDQKSLLLLGRGYQFASALEGALKIKEISYMHSEGVLAGELKHGVLALVDENLPIIAFGTRDSLFPKVVSSIEQVTARKGRPIIICNENDEVWAAKAANSNLATLEVPLTVDCLQGLLNIIPLQLTSYWLAVNKNIDVDFPRNLAKSVTVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGK1</accession>
    <name>C5DGK1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0D05984p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0D05984g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CENP-C/MIF2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552981.1">
        <property type="nucleotide sequence ID" value="XM_002552935.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGK1"/>
    <dbReference type="FunCoup" id="C5DGK1">
        <property type="interactions" value="147"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGK1"/>
    <dbReference type="GeneID" id="8295211"/>
    <dbReference type="KEGG" id="lth:KLTH0D05984g"/>
    <dbReference type="eggNOG" id="ENOG502S47H">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038087_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGK1"/>
    <dbReference type="OMA" id="AKMFFVQ"/>
    <dbReference type="OrthoDB" id="1939643at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019237">
        <property type="term" value="F:centromeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051315">
        <property type="term" value="P:attachment of mitotic spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051382">
        <property type="term" value="P:kinetochore assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051455">
        <property type="term" value="P:spindle attachment to meiosis I kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028386">
        <property type="entry name" value="CENP-C/Mif2/cnp3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025974">
        <property type="entry name" value="Mif2/CENP-C_cupin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028929">
        <property type="entry name" value="Mif2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16684">
        <property type="entry name" value="CENTROMERE PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16684:SF11">
        <property type="entry name" value="CENTROMERE PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11699">
        <property type="entry name" value="CENP-C_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15624">
        <property type="entry name" value="Mif2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mif2 N-terminal" evidence="7">
        <location>
            <begin position="3"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="Mif2/CENP-C cupin" evidence="6">
        <location>
            <begin position="454"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="46"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="344"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="556"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="111"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="186"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="228"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="243"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="380"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="568"/>
            <end position="583"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF11699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF15624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR22543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="583" mass="66287" checksum="DD405D8BC6BB32FA" modified="2009-07-28" version="1">MDYMNLGVKSRKTGIQVKGNIGRDEYSMENIDEFFKDDDTLISARRKSRRSSLLPLSKKFLPEDFSETSRRSSNYLPPSVPTIQEEDDENLHEGSDEPAGGLEINETLSRAVEDNRIHQELSEIPTGDHERRLRQPAEKSYEPDYRAEDDFTTDAVVPQTPPSDYGNESYRDVPDLVADDDDDVSHGNTTFNTSDNALLEDELQREVRAESEEDRDYIERYSNTENSDSSELEDEDNSDTNSPYETHSAQSYNTRVQPALSVSRGRHPLSEPQELSDTSDEEFIYDQARELASENSFTTSAGLRKSKRVKVAPLEYWRNEKVVYKRKSRKPVLEIDKIITYEDEDDEEEEISRRRKSSKKPPYNYGTSGKPRGRPRKSKTTNSASSSGLQNPNEDLISKIRTGDTQCSSWMRRGVFKASVKSSFHGKEENEELLACAPDSVHTEQRRKTADEDFSLAVLFDQHRDLFASGFLTIPTGSCKKLSDSRNAFINFYLIQGVIEVTVGRNKLLCTDGTSFQIPAFNEYAFRNKGKNDAKMYFVQTTIFPRLSQARGIRNLSRSPAEARDNQIPKSTTSPSDMSISEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGN8</accession>
    <name>C5DGN8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">N-alpha-acetyltransferase 40</fullName>
            <ecNumber evidence="4">2.3.1.257</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0D06820g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N-terminal L-seryl-[histone H2A] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-seryl-[histone H2A] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50600"/>
            <dbReference type="Rhea" id="RHEA-COMP:12742"/>
            <dbReference type="Rhea" id="RHEA-COMP:12744"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:64738"/>
            <dbReference type="ChEBI" id="CHEBI:83690"/>
            <dbReference type="EC" id="2.3.1.257"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>N-terminal L-seryl-[histone H4] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-seryl-[histone H4] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50596"/>
            <dbReference type="Rhea" id="RHEA-COMP:12740"/>
            <dbReference type="Rhea" id="RHEA-COMP:12743"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:64738"/>
            <dbReference type="ChEBI" id="CHEBI:83690"/>
            <dbReference type="EC" id="2.3.1.257"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the acetyltransferase family. NAA40 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.257" evidence="4"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553018.1">
        <property type="nucleotide sequence ID" value="XM_002552972.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGN8"/>
    <dbReference type="FunCoup" id="C5DGN8">
        <property type="interactions" value="126"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGN8"/>
    <dbReference type="GeneID" id="8295249"/>
    <dbReference type="KEGG" id="lth:KLTH0D06820g"/>
    <dbReference type="eggNOG" id="KOG2488">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087674_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGN8"/>
    <dbReference type="OMA" id="IYLYEIQ"/>
    <dbReference type="OrthoDB" id="424551at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043998">
        <property type="term" value="F:histone H2A acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010485">
        <property type="term" value="F:histone H4 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990189">
        <property type="term" value="F:protein N-terminal-serine acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039949">
        <property type="entry name" value="NAA40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20531">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20531:SF1">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="13">
        <location>
            <begin position="121"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="40"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="42"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR22580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="260" mass="28729" checksum="8352EE3AADA0E902" modified="2009-07-28" version="1">MVDYPASELFPHVTQCFPLVLTLPNGQQQLTRAVYGIPSDATQHEERREPRAAGPKPEPGPTPASECDSVPRPPADLLDQLLSLVTANLGPMYTRHARAIYGAGRQSWPARKRAEMETPGLVYVVYSDSHSQPLVFMSLLLTDEPELGQELAARVLYLFEIHVSDIIRGQGLGTHLLRDCLGGTLASLSRASPSVLGAELTVFSENQRALRLYLALGMHVAAWSPQDRRAQVRRRTRAIAYDSSLVTRPAYYILFWPAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGV1</accession>
    <name>C5DGV1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DASH complex subunit ASK1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Associated with spindles and kinetochores protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Outer kinetochore protein ASK1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ordered locus">KLTH0D08514g</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="19">Component of the DASH complex consisting of ASK1, DAD1, DAD2, DAD3, DAD4, DAM1, DUO1, HSK3, SPC19 and SPC34, with a stoichiometry of one copy of each subunit per complex. Multiple DASH complexes oligomerize to form a ring that encircles spindle microtubules and organizes the rod-like NDC80 complexes of the outer kinetochore. DASH complex oligomerization strengthens microtubule attachments. On cytoplasmic microtubules, DASH complexes appear to form patches instead of rings.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
            <location evidence="3">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DASH complex ASK1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553081.1">
        <property type="nucleotide sequence ID" value="XM_002553035.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGV1"/>
    <dbReference type="FunCoup" id="C5DGV1">
        <property type="interactions" value="95"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGV1"/>
    <dbReference type="GeneID" id="8295317"/>
    <dbReference type="KEGG" id="lth:KLTH0D08514g"/>
    <dbReference type="eggNOG" id="ENOG502S2V2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090087_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGV1"/>
    <dbReference type="OMA" id="EICERIM"/>
    <dbReference type="OrthoDB" id="5573898at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042729">
        <property type="term" value="C:DASH complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044732">
        <property type="term" value="C:mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008608">
        <property type="term" value="P:attachment of spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013964">
        <property type="entry name" value="DASH_Ask1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28200">
        <property type="entry name" value="DASH COMPLEX SUBUNIT ASK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28200:SF1">
        <property type="entry name" value="DASH COMPLEX SUBUNIT ASK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08655">
        <property type="entry name" value="DASH_Ask1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="16" id="KW-0137">Centromere</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="11" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="9" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="98"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="102"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="186"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="219"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00029735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00029932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00046633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="CAR22643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="251" mass="27304" checksum="07018C65CD7CA07B" modified="2009-07-28" version="1">MAEESLERLDQEITLHLQQIDSNLSYCFSKITQDVIPHVTKYGAVCDKAMDSSNWLKVMFQHAGNLQIDSVTGPQQQQQQQAPEAAENGSLQTLFPEKSRLSPASSSILAPPTAAPHQRPAAGLPVPDLNVTTTTTGKVLQLPDSSDDELGDAADRDPTDRSTTAHSRNDPDGSTLQRQRRKRKLSLLLQQQFGSSSSSVAPSPAQIKRRASSTIDSSPMRKDLASHADSTKQQGVQPGTVIYFPTRDETS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>C5DH99</accession>
    <name>C5DH99_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0E02530p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0E02530g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PI3/PI4-kinase family. TRA1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553597.1">
        <property type="nucleotide sequence ID" value="XM_002553551.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DH99">
        <property type="interactions" value="1329"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DH99"/>
    <dbReference type="GeneID" id="8291739"/>
    <dbReference type="KEGG" id="lth:KLTH0E02530g"/>
    <dbReference type="eggNOG" id="KOG0889">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000129_1_0_1"/>
    <dbReference type="InParanoid" id="C5DH99"/>
    <dbReference type="OMA" id="CLDLYGQ"/>
    <dbReference type="OrthoDB" id="5570127at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05163">
        <property type="entry name" value="PIKK_TRRAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000044">
        <property type="entry name" value="NuA4 histone acetyltransferase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050517">
        <property type="entry name" value="DDR_Repair_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003152">
        <property type="entry name" value="FATC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003151">
        <property type="entry name" value="PIK-rel_kinase_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014009">
        <property type="entry name" value="PIK_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046807">
        <property type="entry name" value="Tra1_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046805">
        <property type="entry name" value="Tra1_ring"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139">
        <property type="entry name" value="ATAXIA TELANGIECTASIA MUTATED ATM -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139:SF1">
        <property type="entry name" value="TRANSFORMATION_TRANSCRIPTION DOMAIN-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02259">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02260">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20175">
        <property type="entry name" value="Tra1_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20206">
        <property type="entry name" value="Tra1_ring"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01343">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51189">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51190">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAT" evidence="5">
        <location>
            <begin position="2594"/>
            <end position="3149"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="4">
        <location>
            <begin position="3352"/>
            <end position="3709"/>
        </location>
    </feature>
    <feature type="domain" description="FATC" evidence="6">
        <location>
            <begin position="3689"/>
            <end position="3721"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="516"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="463"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="516"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="540"/>
            <end position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR23160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="3721" mass="426539" checksum="8186D7992D6DEA77" modified="2009-07-28" version="1">MSYLERIEGLASRLREDNDLAKSLSTISEICEMVEPLGSSAEYEKFLNSILPALMERLESTLYTLDVMSPQQKLRNSILEVLNRCIMNEAFEPFAVKYLDKLLVVLQQDNEENGVLAMKIITGFFKSYKSALQERVTDFIDVIMKIYKHMPELVRQTFNDDLGDNGALDLTGLDNTSEVLDPRGNGEAVQSSDTLAATSKPLKRSMFSFKVLSECPITMVTLYSSYKQLTTSSLPQFMPLVIELLTMEADAQRKTREDFEAQGKRWISVSPNIKNRSAYCDFILSQIKATSFLAYVFIRGYAAEYLQNYVGFVPDLILRLLQDCPAELSAARKELLHATRHILSTNYKKLFLSKINLLFDEKVLIGNGFTSYETLRPLAYSTVADFVHNVRGDLQLDDIEKTIKMYTGFLLDESLALTVQIMSAKLLLNLVERVLKLGKENPNDSVRSKKLLIVIVDAYTTRFRNLNRQYDNLMKHHEEYEKEKMKKSATFRKELEKANEETENFIREMLRVKEPLKQETDAEGDVKMEENVEGHTETSPNDEESEPDNEDTTIDVFDIKFKSPILLSSPPNSDPLKDAFYLYRTLMSFLKTIIHDLKVFNPPSTEYTTMNPKVWTSLSRIFSHEEVVVFKDLFHECILGLQFFSSPEGKTVTDKKHFDIAAPSLPVSATKDGRELMDYFAIMFMQIDSPTFNDIVEAEIDFLYQAMLRDSALLHVAQSFLTSETTSPKFTGILLRFLKSKLSSLGNVDGNKSNILIRLFKLSFMSVNLFPITNEIVLLPHLNDLILDSLKFSTTAEEPLVYLYLIRTLFRSIGGGRFENLYRSIKPILQVLLQSLNRMILTARRPHERDLYVELCLTVPVRLSVLAPYLSYLMKPLVFALQGFPELTTQGLRTLELCIDNLTAEYFDPIIEPVVEDVIRSLFKLLKPQPFNHQISHTAVRILGKLGGRNRRFLKPHSDLRIAEELDIPVNATFRILGINDEVPLSITPGVRSALDILQDYRGEAFYKKSAYNYLKSILQLFLKPSLAIPENYEEWIGGALKVLAQEVVKPPVSQDTEDEKIRDKRLLNSQEMLLAKILEAVIFSASIEELKSEANDLFQNVVNHFCFLQLNNALNKKMGETEKFSLDVKLPKVSIDTNIILDAISEGLSSYMEESRNISVSAVKQIFGTSERIYGSELSLKYSFIPLLVEKFIHQCYDEAYFKKSSGVLGIQTMIEEVNIPVSFLKSYQFDLIAGLLFVLIDTPLEAPGIIRERARKLIISILASTCKNITEESLNEKGLQNTLIDIVCELSNANEAVRSACQDALSTVSEVSGIPIVKLMSHSKNFLLSPIFAKPLRALPFPMQIGNVDAIIYCLSLQGSFLEFNEELYRLLHEAIALVDAEDESLASAQRVTEYQTAEQLVQLRVVCIKLLSLALKNEEFASAQQGATRIRILAVFFKTMLKTSPKIIEATYQGLKSVLAENSKLPKELLQNGLKPMLMNLSDHQKLTVSGLEALAKLLELLIAYFKVEIGKKLLDHLDAWCRVEVLDMLFGKDLKEQTPTKIIYGIINIFHLLPPQADIFLNDLLLKVMLVEKKLRLQLDSPFREPMAKYLNRFHSSVTSYFKNNLGSRDLVLLMCSIIQRPEANNLRQDFEQELNNFYDYYMTGISSNKVRVVSFFANVVEIFYSLERVKGDAWLVNNGSFLYKLRSMLKLTLDIVEENDFHFDFLQLSQATGKVQDLHTRYLELNGNNPAKLFEFLEFAFTSGLKPSQSILSFIEKSVITTSEVETKKLYLHHALLFATSDKPFPATVFVLKNIVNSTLVYEGLTHKSLNRLIKEDDGTQPPWLGLLCSKIWKGSSTILDSFAAGEYDTFRFELLQLSSVFTEYAADLDPAIRKDIIRFCWNFIKLEDALVKQGAYMVTAYFIAKFEFPVKIVTQVFVALLRTSQIESRYMVKRSLDLLAPVMHERMASADPPNAWINWVRRVLSENNASQNSTLYQLLINHPDEFFESRELFIASVINFVGKLTLMTNPNLENQTLVIDLAELILKWEKKAKESDGGDDSVDGNETKEIKQQETFGQREAFVTYLVRYVCVSNHRTSETELGFRTLKVLSEILSENYWPDVTVKLNFFEKFLVQHDLSSSNVSFYCVNALDVLDVILRNKSASWIVSNLVLIQSLLDKCLRSDHHDIQEALQKVLGKVLSAIKHTEESSLESDTETPSKLLLNTLISIITEDLQGTSSVAAGVMLTWTLFMHFPQYVDSLLPSIMKTFSKLCKDHLATSQPKDPATLEESRITTKLLEKVLYLLSMKVALLGDARRPFLSTVALLIDRSMDQNFLKKVIMIARTWVFTNEIFPTVKEKAAILTKMLAFEVRGEPKLSRQFYEIILELFENKDFNNSEITVRMEQPFLVGTRVDDIYIRKRLMAILDESLERDIKERLYYVIRDQNWEFIADYPWLNQALQLLYGAFDKDFSLDLRGAYQLDQLGTALAEITIEKSDTEHEESPLHGLLERHKEFASNFSQVTCNDLVGPLIDIFYRSPQAIHAAWINVFGAAYQSIPKNEKFGFVRSLVTLLSKDYHARQLNVRSNVVSTLLDSISETDSLELPPHLIKYLATSYNSWYQSIKILESLEESASLENAKIIETNEDALLELYVNLQEEDMFYGLWRRRAKYTETNIALSFEQIGLWDKAQQLYEAAQVKARSGALPYSESEYSAWEDNWIMCAEKLQHWDVLTELAKHEGFTDLLLECGWRVADWSADREALEQSVKSVMDVPTPRRQIFETFLALQNFAETKKGDQEVKRLCDEGIQLSLHAWASMPERFTPAHKFLLHSFQQYMEFLEATQVYANLASTSIQNLDTKAQEVKRVLQAWRDRLPNIWDDINMWNDLITWRQHAFQVINNAYLPLVPALQQASNNSNVNTHAYRGYHEIAWVINRFAHVARKHNMPDVCISQLARIYTLPNIEIQEAFLKLREQAKCHYQNMNELTTGLDVISNTNLVYFGTVQKAEFFTLKGMFLSKLRAHDEANQAFATAVQIDLNLAKAWAQWGFFNDRRLSEEPQNISFANNAISCYLQAAGLYNNSKTRKLLCRILWLIGIEDSTGTLAGAFESFRGEIPVWYWITFIPQLLTSLSHKEANMVRQILIRIAKSYPQALHFQLRTTKEDFSVIQRQTMVAMGNSRLPNNKSPATASGTRQPWEYLDELNGILKTAYPLLALSLESLVDQINQKFKTTSDEDLFRLINVLLIDATYNYNRLPFPRENPLLPASTETNLVRFSENLLPPHISVKFNADFIDSKPNFEEYIKRLRYWRNRLENKLDRAPKIENMEKWCPHLSNFHHQKFEDIEIPGQYLLNKDSNAHFVKISRFLPHVEFVRGTHSSYRRLTIRGHDGSLHSFAVQYPAVRHSRREERMFQLFRLFNETLSKNVQTRRRNVQFTLPISVPLSPQVRIMNDSSSNTTMHNLYDEYCDKKGIDRGAIQDFVCQQLDAAYDKVLPPPEITAVKVEIFSSIQSMFLPSSVMKNYFTGLFTQFEDFWLFRKQFSSQYAMFIFMTYMLAINNRAPHKISIDQVSGNVFTLEMLPARYPYERVKQTAKNFEADIPPDSPVFHNNEAVPFRLTPNIQKLIGESALEGILSVNIFLVARALLEPDHELNTYLSLFIRDEVISWYSNLHRSIVEDPQLREIVRTNVDLITRKTAQLGHLSSKPSVVTQYVLEAISAAVSPRNLAKMDQSFMPYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHC7</accession>
    <name>C5DHC7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0E03234p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0E03234g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Allantoate permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553625.1">
        <property type="nucleotide sequence ID" value="XM_002553579.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHC7"/>
    <dbReference type="GeneID" id="8291770"/>
    <dbReference type="KEGG" id="lth:KLTH0E03234g"/>
    <dbReference type="eggNOG" id="KOG2533">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_0_5_1"/>
    <dbReference type="InParanoid" id="C5DHC7"/>
    <dbReference type="OMA" id="QYQWLGS"/>
    <dbReference type="OrthoDB" id="6730379at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17327">
        <property type="entry name" value="MFS_FEN2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000064">
        <property type="entry name" value="MFS allantoate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF1">
        <property type="entry name" value="ALLANTOATE PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="115"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="206"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="239"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="342"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="371"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="398"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="429"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="462"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="78"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR23188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="521" mass="57927" checksum="550E3F27B3B5754E" modified="2009-07-28" version="1">MLSQFKPNSPVFEMEKSCEKQHKVMTSSEEVALGEFEEGPLCSKAGVDLGMEAAQESMGLQVDEVTNKRLLRKADCYICTMMSVVYAVQYMDKQTNSYASIMGLRKDLKMHGNQYSWVGTCFYLGYMIFEIPSSLALQRFPIAKVSAVFIVLWGFVLCMTSLPKNYASFIATRTVLGILESAVTPAFMLLTSQWYRREEQFLRTSIWSASAGLGAILGSLIAYGLAVRDPSSLPIASWRLLYVVLGVVTIVLGVAFFFVPDTPASAWFLTKEERLLTVERIRSNKQGFGNRHFKKNQLREVFMDIRTYIYFATMVAAEIPNGGIGNFGSIMLTDMGYNSKRALLMGTPFGAVEFGGIILVGYIAQKKQKRMAAAFFSYCLNIVSGCLLAFPSNTNAQLAGYYLQGMAVIGWICFISCVSSNTAGHTKKVVTSAICMIGYCVGNLIGPQTFKSAEAPHYKSAKIAIVVCFVICLFLTVLLYCINVRANKIRDEKNEKLDSSFVNSEFADLTDFENPEFRYSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHD5</accession>
    <name>C5DHD5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Helicase SWR1</fullName>
            <ecNumber evidence="4">3.6.4.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Helicase swr1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="ordered locus">KLTH0E03476g</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalytic component of the SWR1 complex which mediates the ATP-dependent exchange of histone H2A for the H2A variant HZT1 leading to transcriptional regulation of selected genes by chromatin remodeling.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17 26">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the SWR1 chromatin-remodeling complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family. SWR1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="4"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553633.1">
        <property type="nucleotide sequence ID" value="XM_002553587.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DHD5">
        <property type="interactions" value="253"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHD5"/>
    <dbReference type="GeneID" id="8291781"/>
    <dbReference type="KEGG" id="lth:KLTH0E03476g"/>
    <dbReference type="eggNOG" id="KOG0391">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000315_24_4_1"/>
    <dbReference type="InParanoid" id="C5DHD5"/>
    <dbReference type="OMA" id="AFQQWFG"/>
    <dbReference type="OrthoDB" id="372624at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000812">
        <property type="term" value="C:Swr1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18003">
        <property type="entry name" value="DEXQc_SRCAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000005">
        <property type="entry name" value="Photoperiod-independent early flowering 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000655">
        <property type="entry name" value="Protein PHOTOPERIOD-INDEPENDENT EARLY FLOWERING 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.850">
        <property type="entry name" value="SWI2/SNF2 ATPases, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014012">
        <property type="entry name" value="HSA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050520">
        <property type="entry name" value="INO80/SWR1_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45685:SF1">
        <property type="entry name" value="HELICASE SRCAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45685">
        <property type="entry name" value="HELICASE SRCAP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07529">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00573">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51204">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0010">Activator</keyword>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HSA" evidence="23">
        <location>
            <begin position="298"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="21">
        <location>
            <begin position="668"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="22">
        <location>
            <begin position="1207"/>
            <end position="1357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="116"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="423"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="480"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1424"/>
            <end position="1456"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="343"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="139"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="425"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="446"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="511"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="531"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="595"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="1442"/>
            <end position="1453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00037570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00040599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00074297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="CAR23196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1474" mass="168943" checksum="E5933E5C8A78E323" modified="2009-07-28" version="1">MTVAPNIDEQIADLKFNYEVLINELFHLKEFTSLVEYDPCFRNSSESFEHFVKTSGLGLSKPAIEAVEDASASRRVRRRQSRRETIPEASCSESVEGLIDERFHELECQMTNKKYKRQSPAIRELSAPKKRKAAVKVEAPPKESIKKEEEKIAPPSPPKLHHKSSSIDNEQVSDKGYNSMCYTTSSSEDEAENRRTKRKRPRVNLIVNIPKQTVTNPLHVTKPEFGSLSEFLQSYKSLDEDMSIEEFNKFIEEQRRLFANIRKGLESGVLKYDPATDSIEPITLKDAAGVHAHRPEPITYFYKEQQQYTFQDHLINQGIVMSRAFQETKKARISRIRKITQMIEQHFRHIAGAEERRQKEEEKRLKTMARTAVQAVKRRWVTAEKAYKVLKKDEEDQLKKIQGKEHLSKMLEQSTQLLGAQLKRPNQDDTEKDLNTSASHSDVDQSDSNSDSFTSSDEEDSASGNEDDARLSVEQLQMKYANLDSSPKSEDEESKTEREDSMDAAPVNLLSEKERLELKKDLEDKSLGLLDESDDEDTDSMSEHSSEPDSGEDEQGSEATSESVPKSDDGLASIFNNIEEGESEVESDYASSTSAEEDDAFDSEESADDNSQEDLDHSANVGYEKKPEPQGGQLVPEKDSLTVVDVPIPSLLRGTLRIYQKQGLNWLASLYNNNTNGILADEMGLGKTIQTISLLAYLACEKQNWGPHLIIVPTSVLLNWEMEFKRFAPGFKVLTYYGSPQQRKDKRKGWNRPDAFHICITSYQLVVHDQHSFKRKKWQYMILDEAHNIKNFRSTRWQALLNFNTERRLLLTGTPLQNNLAELWSLLYFLMPQTAVGNNGGIQGFADLEAFQQWFGRPVDKIIQIGEGYAQDEETRKTVTKLHQVLRPYLLRRLKADVEKQMPAKHEHIVYCRLSKRQRFLYDDFMSRAQTKETLASGNFMSIINCLMQLRKVCNHPDLFEVRPILTSLCVEKSVAHDYVYLNNLVSQKLHAMDHEREIDLEVLNLKFTSNDWSLSTHHAASINKSQCVEQFVKEVSKLRAETEEQLKMGASESALNFQDINQFYRTFAAKKTDDMMGRLEHLQYLNNMRCTRKPVYGNNLIKLLSINKPVREGPLKELCRPLQTRILDSDALIDKFAVITPTVVSLDSRNMALGLNDESEFHSNLREDLKQVLRRTDNPLHKLQTKLAIAFPDKSLLQYDCGKLQKLATLLRDLKDGGHRALIFTQMTKVLDVLEQFLNYHGYLYMRLDGATKIEDRQILTERFNTDNRITAFILSSRSGGLGINLTGADTVIFYDSDWNPAMDKQCQDRCHRIGQTRDVHIYRFVSEHTIESNILKKANQKRQLDNVVIQRGDFTTDYFTKLSVKDLVGAEVPEIEANNRPLLMDADAATKDPRKLEKLLAQAEDADDVKAAKLAMKEVEVDNEDFQEVKNGPQGPKEDDASEEDDPDDEYQGTRHVEEYMIRFIANGWYWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHG5</accession>
    <name>C5DHG5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0E04158p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0E04158g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553663.1">
        <property type="nucleotide sequence ID" value="XM_002553617.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHG5"/>
    <dbReference type="FunCoup" id="C5DHG5">
        <property type="interactions" value="52"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHG5"/>
    <dbReference type="GeneID" id="8291812"/>
    <dbReference type="KEGG" id="lth:KLTH0E04158g"/>
    <dbReference type="eggNOG" id="ENOG502QWMQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045029_0_0_1"/>
    <dbReference type="InParanoid" id="C5DHG5"/>
    <dbReference type="OMA" id="WLENRWI"/>
    <dbReference type="OrthoDB" id="189226at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036149">
        <property type="term" value="P:phosphatidylinositol acyl-chain remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10983">
        <property type="entry name" value="1-ACYLGLYCEROL-3-PHOSPHATE ACYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10983:SF70">
        <property type="entry name" value="PROTEIN MUM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR23226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="469" mass="53652" checksum="19C236F49141324E" modified="2009-07-28" version="1">MSILDTLRTHFVGSGLQLRADNVPAALSLVLQLVLLVQYVAAYNVWSFKTWIVEQSVDSRPSKWAIGTVSKTLIWIPLVGERLTENLEHVVCSIKLRVLPPMKRVEERLELCYQVALADLTFRHGNVRLLVTGDTEGFPTAVTNKRSSLVIANHRSVMDYSLINYLAQGEERVAARQNFWRLFESKETDTMLGLKFVTWGRVCNMPSLRLLSSILAGDENCAVDAARLQEFLEDRGNQILAVFPEVNVLTPELRLVQRKLAKDNYLPMLNNVLYPRFKNFKAAIRCLSHVRGVGIARRGRRLRNAVTKADSMVCKIRHPGSRISDTQIAQVSMFLGRVEDDEALAKNKKKLKPTQRALLSVDPFVWDLTIVYYRAKISKGKDHAHLHEQEQKRLEDGAHYQLEQVTPSFLQMASSSFQDSPLLIRIHTCKHPMSKLLALSDRKLEKWLERRWMQKDEMIDSIQKNVKIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHH3</accession>
    <name>C5DHH3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Serine/threonine-protein kinase STE11</fullName>
            <ecNumber evidence="2">2.7.11.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">KLTH0E04334g</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Serine/threonine protein kinase required for cell-type-specific transcription and signal transduction in yeast. It is thought that it phosphorylates the STE7 protein kinase which itself, phosphorylates the FUS3 and or KSS1 kinases.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.25" evidence="2"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553671.1">
        <property type="nucleotide sequence ID" value="XM_002553625.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHH3"/>
    <dbReference type="FunCoup" id="C5DHH3">
        <property type="interactions" value="220"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHH3"/>
    <dbReference type="GeneID" id="8291820"/>
    <dbReference type="KEGG" id="lth:KLTH0E04334g"/>
    <dbReference type="eggNOG" id="KOG0198">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003051_2_1_1"/>
    <dbReference type="InParanoid" id="C5DHH3"/>
    <dbReference type="OMA" id="CFNADSI"/>
    <dbReference type="OrthoDB" id="266718at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004709">
        <property type="term" value="F:MAP kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019236">
        <property type="term" value="P:response to pheromone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09534">
        <property type="entry name" value="SAM_Ste11_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000334">
        <property type="entry name" value="Serine/threonine-protein kinase STE11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000272">
        <property type="entry name" value="Serine/threonine-protein kinase STE11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029458">
        <property type="entry name" value="Ras-bd_By2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11584:SF369">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 19-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11584">
        <property type="entry name" value="SERINE/THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14847">
        <property type="entry name" value="Ras_bdg_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01304">
        <property type="entry name" value="Ras_bdg_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0589">Pheromone response</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM" evidence="17">
        <location>
            <begin position="3"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="450"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="366"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="372"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="479"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00056321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00070094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAR23234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="742" mass="82453" checksum="3146B993DE424F7D" modified="2009-07-28" version="1">MDDSETYVSHFLQELNCHQYLDSFKNWNLTSQDALAHIDKEILVEVGVKKIGDRIRILKKCQSLRGSRTRPSHEVRELLQKFQALSVAQLTTTEELVTDKHSAIFILNDGSAKKVNVNGCFNADSIKKKLIKKLPSEFIACTPQGEETRNSQDYDVFVVDYVKNVLHLLYDVELVTICHSVDRVEKNRLIFVSKDQTPSDRATLTSKKLYLKTLSVIHQLGSLSALAGTTSSARAKIHAVNELAAPGTAAGAPNSLRIENNKDTIRQIFNQRPPSELISTNLSEYFPHTDAKSLRKTIGNSVRQSIRLSSINGSATPVGGNNVGDIWVNNSNAVGKALLQSLDGNHHVPTGSSLSKRRTQIEEDTLNFKAPESAHGESEVSKNSEGTTERRTGPTKRPGLISHAEYLRQGPRKQKSSERIELLRVADSDNDEEDEDMISLPTKIATPKNWLKGARIGAGSFGSVYLGMNAQTGELMAVKQVELQPTAVTAGVVSVSDEIKKQYNQNANASAVKNSSQVHRKMIDALQHEMGLLKELHHENIVTYYGSSQEGGNLNIFLEYVPGGSVSSMLNSYGPFEEPLIKNFTRQILIGLSYLHRKNIIHRDIKGANILIDIKGCVKITDFGISKKLSPLNQQQNKRASLQGSVYWMAPEVVKQVVTTKKADIWSVGCVIIEMFTGKHPFPDFSQMQAIFKIGTNTHPESPSWASDEAKNFLLKAFELDYRQRPDSIELLQHAWLDTSIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-04-02" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHI8</accession>
    <name>C5DHI8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0E04664p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0E04664g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553686.1">
        <property type="nucleotide sequence ID" value="XM_002553640.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHI8"/>
    <dbReference type="STRING" id="559295.C5DHI8"/>
    <dbReference type="GeneID" id="8291835"/>
    <dbReference type="KEGG" id="lth:KLTH0E04664g"/>
    <dbReference type="eggNOG" id="ENOG502S1Z7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_586689_0_0_1"/>
    <dbReference type="InParanoid" id="C5DHI8"/>
    <dbReference type="OMA" id="VRPDMVF"/>
    <dbReference type="OrthoDB" id="5344169at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BHLH" evidence="2">
        <location>
            <begin position="403"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="110"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="318"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="355"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="154"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="181"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="234"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="267"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="365"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="402"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR23249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="466" mass="50172" checksum="087659D0CD1758B5" modified="2009-07-28" version="1">MNEAESFDSKSILDQVDQYLSDQSVRQGEQPGAAPFQELSFHALPHGHEHNQDAEHGSEKGREHGHSFSQGDHSAHLGHGFQLELEVATHSHVRPDMVFSPVISPVVAPQHPSHTPFLRSQASGMSGGASTKPSFSPLSSPAIDAQFPEYPGAKRGSSSSSSRGKKTPLSTPALTATTFSNSSGSKVAKNSPQLSSRRSALSKRKSAATNWDDMFKLPDSSIPPPSVPLFGSAQQQQQQQQQQQQQQPQNTSHLHTHSLSESADDTVSGSCSSASSVRARASGTPALPSRDPDGAGHNDFNVTPATLMNYPKIILPSNSRDVGSHAQSPVRDQSTYAHSGNGHVIMATDSPVIQAKNPNLPLSRHASNTSIPTRSTPELRPVRKSSKSSLVDSGPDPDEDEQAKKEVHKAAEQGRRNRLNTALAELHTLVPAEMKEAVLIPSKATTVEMACTYIRQLIQRVEELQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHU2</accession>
    <name>C5DHU2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0E07128p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0E07128g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553790.1">
        <property type="nucleotide sequence ID" value="XM_002553744.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHU2"/>
    <dbReference type="FunCoup" id="C5DHU2">
        <property type="interactions" value="812"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHU2"/>
    <dbReference type="GeneID" id="8291945"/>
    <dbReference type="KEGG" id="lth:KLTH0E07128g"/>
    <dbReference type="eggNOG" id="KOG0671">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_5_8_1"/>
    <dbReference type="InParanoid" id="C5DHU2"/>
    <dbReference type="OMA" id="YKNHICL"/>
    <dbReference type="OrthoDB" id="283111at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043484">
        <property type="term" value="P:regulation of RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14134">
        <property type="entry name" value="PKc_CLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051175">
        <property type="entry name" value="CLK_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45646">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE DOA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45646:SF11">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE DOA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="245"/>
            <end position="677"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR23353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="692" mass="78746" checksum="EDA9C68504876B07" modified="2009-07-28" version="1">MSQHAQVKRKRTRTLDAAPQAMGVASGVSPGMPQGMFLDNDADPVIGEMLARQSALLQGNLIDTHFSDEPAPRALPQSTLGFHSFTDARDDTEQDDDLIFVKEQPVGGPSSSSAVALDQPQFDHHYTAKKFKKQRTISLPQLPYAKLLYEARGTVPHRQNDDDLLHLSGSSTKTIDGNRAVTLNVVRSVSPLTSQDHAPVLKRMAAAPKISKLVPSRKKKKKFFQTDKEGHYVFQQNDVFCNNRFIVKELLGQGTFGKVVQCVDTAPPVSPHVSDFQPFEDPFAESFEDSHFDLDYGHSQSNDSQPRLVAVKIIRAVDRYREAAKTELRVLKAIIENDSTGQYQCLLLRECFDYKNHICLVTDLFGKSVYDFMCNNGCPRFPGSHVQAISKQLIRSVCFLHDLGIIHTDLKPENVLLCDESYVGKPLSPQTYSSLSPRRKSACGGERRFLTNPEVKLIDFGSAVFHNEFHPAIISTRHYRAPEIVLGLGWSFPCDIWSIGCVLVELVTGESLYPIHENLEHMAMMQRFNGRPFPPKIVENMFYKADRKLGNMSPDLNSTVIKHFDRDTLALQWPERNKKGEIITKERSMKRILTSCDRLDKYILQKVRQDYGDWLTINWDLSPEDNWALLESKYDEQVLDREVFLFWYWFLDLCKKMFEFDPTKRITAREAIGHEWFNYGILDEGIASFGKV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-11-03" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHW0</accession>
    <name>GET1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Golgi to ER traffic protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Guided entry of tail-anchored proteins 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">GET1</name>
        <name type="ordered locus">KLTH0E07568g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Together with GET2, acts as a membrane receptor for soluble GET3, which recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. The GET complex cooperates with the HDEL receptor ERD2 to mediate the ATP-dependent retrieval of resident ER proteins that contain a C-terminal H-D-E-L retention signal from the Golgi to the ER.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the Golgi to ER traffic (GET) complex, which is composed of GET1, GET2 and GET3. Within the complex, GET1 and GET2 form a heterotetramer which is stabilized by phosphatidylinositol binding and which binds to the GET3 homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the WRB/GET1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553808.1">
        <property type="nucleotide sequence ID" value="XM_002553762.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHW0"/>
    <dbReference type="SMR" id="C5DHW0"/>
    <dbReference type="FunCoup" id="C5DHW0">
        <property type="interactions" value="45"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHW0"/>
    <dbReference type="GeneID" id="8291965"/>
    <dbReference type="KEGG" id="lth:KLTH0E07568g"/>
    <dbReference type="eggNOG" id="KOG4253">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089418_2_1_1"/>
    <dbReference type="InParanoid" id="C5DHW0"/>
    <dbReference type="OMA" id="AQDNYAR"/>
    <dbReference type="OrthoDB" id="69461at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043529">
        <property type="term" value="C:GET complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043495">
        <property type="term" value="F:protein-membrane adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071816">
        <property type="term" value="P:tail-anchored membrane protein insertion into ER membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.660">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03113">
        <property type="entry name" value="Get1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028945">
        <property type="entry name" value="Get1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027538">
        <property type="entry name" value="Get1_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029012">
        <property type="entry name" value="Helix_hairpin_bin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42650:SF1">
        <property type="entry name" value="GUIDED ENTRY OF TAIL-ANCHORED PROTEINS FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42650">
        <property type="entry name" value="TAIL-ANCHORED PROTEIN INSERTION RECEPTOR WRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04420">
        <property type="entry name" value="CHD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0175">Coiled coil</keyword>
    <keyword id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword id="KW-0931">ER-Golgi transport</keyword>
    <keyword id="KW-0333">Golgi apparatus</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000388597" description="Golgi to ER traffic protein 1">
        <location>
            <begin position="1"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="topological domain" description="Lumenal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="6"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="1">
        <location>
            <begin position="24"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="topological domain" description="Lumenal" evidence="1">
        <location>
            <begin position="126"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="170"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="1">
        <location>
            <begin position="187"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="67"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03113"/>
        </source>
    </evidence>
    <sequence length="195" mass="22164" checksum="B88B41D9149B088B" modified="2009-07-28" version="1">MVNSTILVTVVLVLALRALQWCSGYQHKFIDMIWCKPVALKLQGLIKKRRELHLAQQSTSAQDEYAKWTKLNRQIAQLDTQVKQTQEQLVENRKVGEKNLGKLRLVFFTAPLLVLRFWKGKLPVYALPSGMFPRVVESVLSQGWAAAALAPVRFVWASGTVKPMQVETPVCLAIWLWALSRVLDTSEFVVRSLCM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHX7</accession>
    <name>C5DHX7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Plasma membrane ATPase</fullName>
            <ecNumber evidence="17">7.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ordered locus">KLTH0E07942g</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The plasma membrane ATPase of plants and fungi is a hydrogen ion pump. The proton gradient it generates drives the active transport of nutrients by H(+)-symport. The resulting external acidification and/or internal alkinization may mediate growth responses.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 17">
            <text>ATP + H2O + H(+)(in) = ADP + phosphate + 2 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:20852"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 17">Cell membrane</location>
            <topology evidence="2 17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIIA subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.1" evidence="17"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553825.1">
        <property type="nucleotide sequence ID" value="XM_002553779.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHX7"/>
    <dbReference type="FunCoup" id="C5DHX7">
        <property type="interactions" value="483"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHX7"/>
    <dbReference type="GeneID" id="8291982"/>
    <dbReference type="KEGG" id="lth:KLTH0E07942g"/>
    <dbReference type="eggNOG" id="KOG0205">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002360_6_0_1"/>
    <dbReference type="InParanoid" id="C5DHX7"/>
    <dbReference type="OMA" id="VIEFHPF"/>
    <dbReference type="OrthoDB" id="116380at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008553">
        <property type="term" value="F:P-type proton-exporting transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120029">
        <property type="term" value="P:proton export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02076">
        <property type="entry name" value="P-type_ATPase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000011">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000005">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000008">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006534">
        <property type="entry name" value="P-type_ATPase_IIIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01647">
        <property type="entry name" value="ATPase-IIIA_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 17" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="14 17" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 17" id="KW-1278">Translocase</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 17" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="95"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="124"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="312"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="675"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="702"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="742"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="808"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="841"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="19">
        <location>
            <begin position="52"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="18"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU362083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CAR23388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="901" mass="98276" checksum="8774D13C6D76D71B" modified="2009-07-28" version="1">MSAATTEPTKEKPVQPVESDEEDDDIDALIDELQSNHNLDEEDEEDDVKVAAGEARPVPEELLQTDPSYGLTSDEVTKRRKRYGLNQMAEESESLIVKFLGFFIGPIQFVMEAAAILAAGLEDWVDFGVILGLLFLNAAVGFIQEYQAGSIVDELKKSLANSAVVIRDGNLVEIPANEVVPGDIMQLEDGTVICADGRLVTEECFLQIDQSAITGESLAVDKHYGDTTFSSSTVKRGEGFMIVTATGDNTFVGRAAALVNQASGDQGHFTEVLNGIGTILLVLVIVTLLLVWTACFYRTVRIVRILRYTLGITIIGVPVGLPAVVTTTMAVGAAYLAKKQAIVQKLSAIESLAGVEILCSDKTGTLTKNKLSLHEPYTVEGVEPDDLMLTACLAASRKKKGLDAIDKAFLKSLAQYPRAKNALTKYKVLDFHPFDPVSKKVTAVVESPEGERIICVKGAPLFVLKTVEEDHPIPEDVHENYENKVAELASRGFRALGVARKRGEGHWEILGVMPCMDPPRDDTAQTVNEARRLGLRVKMLTGDAVGIAKETCRQLGLGTNIYNAERLGLGGGGDMPGSELADFVENADGFAEVFPQHKYAVVEILQQRGYLVAMTGDGVNDAPSLKKADTGIAVEGATDAARSAADIVFLAPGLSAIIDALKTSRQIFHRMYSYVVYRIALSLHLEIFLGLWIAILNNSLDIDLIVFIAIFADVATLAIAYDNAPFDQKPVKWNLPRLWGMSIILGVILAVGTWLTLTFMFVPKGGIIQNFGSIDGVLFLQISLTENWLIFITRAVGPFWSSIPSWQLSGAVFIVDIIATMFCLFGWWSQNWNDIVTVVRVWVFSFGVFCVLGGAYYLMSGSVAFDRLMNGKPMKEKPPTRSVEDFLVAMQRVSTQHEKGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>C5DI17</accession>
    <name>C5DI17_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0E08976p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0E08976g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553865.1">
        <property type="nucleotide sequence ID" value="XM_002553819.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DI17"/>
    <dbReference type="GeneID" id="8292028"/>
    <dbReference type="KEGG" id="lth:KLTH0E08976g"/>
    <dbReference type="HOGENOM" id="CLU_2386569_0_0_1"/>
    <dbReference type="InParanoid" id="C5DI17"/>
    <dbReference type="OrthoDB" id="10462761at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="94" mass="10846" checksum="E1EAE8706BBE1301" modified="2009-07-28" version="1">MSAVWFYPNDNWSIGPTQRLGIYRGTAPKATELNWEANPRRSNHPKNSRSHAELDLSTRRRGCFCLIRYTRLRQQPLTCCSSPHDSDPAEAEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DI43</accession>
    <name>C5DI43_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0E09570p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0E09570g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA repair metallo-beta-lactamase (DRMBL) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553891.1">
        <property type="nucleotide sequence ID" value="XM_002553845.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DI43"/>
    <dbReference type="FunCoup" id="C5DI43">
        <property type="interactions" value="236"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DI43"/>
    <dbReference type="GeneID" id="8292055"/>
    <dbReference type="KEGG" id="lth:KLTH0E09570g"/>
    <dbReference type="eggNOG" id="KOG1361">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005260_7_0_1"/>
    <dbReference type="InParanoid" id="C5DI43"/>
    <dbReference type="OMA" id="QIFKVPY"/>
    <dbReference type="OrthoDB" id="262529at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035312">
        <property type="term" value="F:5'-3' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16273">
        <property type="entry name" value="SNM1A-1C-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12650">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011084">
        <property type="entry name" value="DRMBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23240:SF6">
        <property type="entry name" value="DNA CROSS-LINK REPAIR 1A PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23240">
        <property type="entry name" value="DNA CROSS-LINK REPAIR PROTEIN PSO2/SNM1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07522">
        <property type="entry name" value="DRMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA repair metallo-beta-lactamase" evidence="6">
        <location>
            <begin position="439"/>
            <end position="571"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR23454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="595" mass="68537" checksum="C2FA6BAC396F7E7D" modified="2009-07-28" version="1">MQSNKRQRTLVDFDIPKTDSLVPRHVKKRQKSPIFIDLETESYHEHILEENIAVKDEQQAVRNNLFVADEDQEDNSEAVEVISDVTKPSPKAQDLRLALSGNQNDAKPKPVRKQKNSFTESLTTVGQKLVSVTEVGEEQQVLCPVCGIVLTKLEIHEREAHCDHCIEKDAAPSKIRTGRRIPRLPEVKKVRFKNHVINVDGFNFQSDPSIHQYFLSHFHADHYMGVKKSWDQGTIYCSKVTADLLIYKFKVPQDRIIALPAEVTIQISSNVSVICFDANHCPGAFVFLFREFGGDNETVQWVLHTGDFRSNNDLITKIIEHTSGKPIDKVYLDTTYMYPSYHFPLQKSVLDVTGEFAFKLRDVGLKKLFGDRQSSIMSFVSNSLRPRHLYKYVFVIGTYTIGKEKLAIAIAERLQTKIFVARDTARHCIIQTYKDKFPEGMITHDITKACVHLVSLRTLSSKETLQYYFKPISHIYEDMVAFAPTGWSFKNGGRFIKLHETLEQKIEHTVNLLKDSTVDNLDPSSIHKQYKRDVRFQVFQVPYSEHSSFKDLANFCVRLPWVKIIPTVNTHDDYMVSQMREWFKAWKNVHGQRTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>C5DI45</accession>
    <name>C5DI45_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Malate dehydrogenase</fullName>
            <ecNumber evidence="3 12">1.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0E09614g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 12">
            <text>(S)-malate + NAD(+) = oxaloacetate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21432"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.37"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the LDH/MDH superfamily. MDH type 1 family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.37" evidence="3 12"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553893.1">
        <property type="nucleotide sequence ID" value="XM_002553847.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DI45"/>
    <dbReference type="FunCoup" id="C5DI45">
        <property type="interactions" value="237"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DI45"/>
    <dbReference type="GeneID" id="8292057"/>
    <dbReference type="KEGG" id="lth:KLTH0E09614g"/>
    <dbReference type="eggNOG" id="KOG1494">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047181_1_1_1"/>
    <dbReference type="InParanoid" id="C5DI45"/>
    <dbReference type="OMA" id="FQAGKVM"/>
    <dbReference type="OrthoDB" id="4069699at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030060">
        <property type="term" value="F:L-malate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006108">
        <property type="term" value="P:malate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01337">
        <property type="entry name" value="MDH_glyoxysomal_mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000013">
        <property type="entry name" value="Malate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.110.10:FF:000009">
        <property type="entry name" value="Malate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.110.10">
        <property type="entry name" value="Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001557">
        <property type="entry name" value="L-lactate/malate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022383">
        <property type="entry name" value="Lactate/malate_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001236">
        <property type="entry name" value="Lactate/malate_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015955">
        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001252">
        <property type="entry name" value="Malate_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010097">
        <property type="entry name" value="Malate_DH_type1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01772">
        <property type="entry name" value="MDH_euk_gproteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11540">
        <property type="entry name" value="MALATE AND LACTATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11540:SF72">
        <property type="entry name" value="MALATE DEHYDROGENASE, PEROXISOMAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02866">
        <property type="entry name" value="Ldh_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00056">
        <property type="entry name" value="Ldh_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000102">
        <property type="entry name" value="Lac_mal_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56327">
        <property type="entry name" value="LDH C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00068">
        <property type="entry name" value="MDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10 12" id="KW-0520">NAD</keyword>
    <keyword evidence="5 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Lactate/malate dehydrogenase N-terminal" evidence="13">
        <location>
            <begin position="2"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Lactate/malate dehydrogenase C-terminal" evidence="14">
        <location>
            <begin position="146"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="181"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="8"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="116"/>
            <end position="118"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR23456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="337" mass="36129" checksum="93BCB6780A818394" modified="2009-07-28" version="1">MTKVTVLGAAGGIGQPLSLLLKMSPYVSTLALYDLRLAPGVARDLSHINTNSKCRGYGKDELADALKGANVVVIPAGVPRKPGMTRDDLFKINAGIVANLVSATAKYAPSARILVISNPVNSTVPVACETLKNLGQFKPGKVMGVTTLDVVRAHTFLSDALGADHYGREAMKSHVTVIGGHSGTTIVPIVTNAKVRSKLVNKRQYDAYIHRVQFGGDEVVEAKQGAGSATLSMALAGFQFTNEVLRSIHLEKRAEPGPLPAFIYLPGVEGGKELQSKLGTNIEYFAAPVQMLRGDVVRVDVDWVDGLSDEEKKAIKICLEELDKNVQKGKRFVQSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIC6</accession>
    <name>C5DIC6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Probable vacuolar protein sorting-associated protein 16 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0E11462g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Essential for vacuolar protein sorting. Required for vacuole biogenesis, stability and to maintain vacuole morphology.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the VPS16 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553974.1">
        <property type="nucleotide sequence ID" value="XM_002553928.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIC6"/>
    <dbReference type="FunCoup" id="C5DIC6">
        <property type="interactions" value="1129"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIC6"/>
    <dbReference type="GeneID" id="8292141"/>
    <dbReference type="KEGG" id="lth:KLTH0E11462g"/>
    <dbReference type="eggNOG" id="KOG2280">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008909_1_0_1"/>
    <dbReference type="InParanoid" id="C5DIC6"/>
    <dbReference type="OMA" id="YVTFWYP"/>
    <dbReference type="OrthoDB" id="1792at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030897">
        <property type="term" value="C:HOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.780">
        <property type="entry name" value="Vps16, C-terminal region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016534">
        <property type="entry name" value="VPS16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006925">
        <property type="entry name" value="Vps16_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038132">
        <property type="entry name" value="Vps16_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006926">
        <property type="entry name" value="Vps16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12811">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING VPS16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12811:SF0">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 16 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04840">
        <property type="entry name" value="Vps16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04841">
        <property type="entry name" value="Vps16_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007949">
        <property type="entry name" value="VPS16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0653">Protein transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Vps16 N-terminal" evidence="4">
        <location>
            <begin position="6"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="Vps16 C-terminal" evidence="3">
        <location>
            <begin position="483"/>
            <end position="798"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR007949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR23537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="799" mass="92327" checksum="367F55BBB556EB5D" modified="2009-07-28" version="1">MGVKNPSLGWEKLKDVYYRNRELCQLSLPKEDQYKLIISTTVLAIEVEESIRVFQYKGEQLGVIELNKLPSELVCYELDSFDEGSLVLALSDRIRVYHNWSPLLFQDFLLPDTIQDKIWDYKNRVLVLSSSQDLFYFDGLGLQVLLKNTENFTLLTKNHWHCNEDIVVLLDLDHVFHLNIKSLELVPMFMGETWHTVSVSPRGLVCLFNAKTNELRIYKDSKTKLMELNLETQPNDMAWCGDDTIACAFNHEEIRLYGPDSEYVTFWYPDEIIVLRGESNGLRLITSEKVELISKVQQFTSNIFLMGSTEPSAILLDSVNLLSTQAPRALENLKIINLDQGVAECLDAALEELDPYWQKKLLAAAAFGKSSLPKNTQLSKVFVETCNKLRVLNVLTEMGIILTSSNLLVITLDGLIARLIKTGNFYECIQICKFLKDRGRLASIFKSWANAKIAFSADLDDEDLFKVIIRLAEGLSVQLPLAEIGLAAFSEGRPALAKQLVLKEPLPDLELPSLLELDEHELALKEGRRRGIPGMIMSILLILQKQLTTSQFTKVIMLVMQDNQLYTYYARHDDEFLFDFYRQTDQLSNLAHHIWDQEVRNQNPAEYLRQVEELYNRDSQDILIKNDQSLLHRQSELYEFQRSISHLLQVDLENMNLDGTIELLIKMKLDKQLNSLIKKFKVTDRKIYHIKCRVLTQQDRFDDLFKFAQERKSPIGYHPFFKYCLKQKRKKEAAVYVRMISGIPYEKRIEMYLRCESYNDAINLASKEKDIPSLKNIYKTIPPNQPQLKALVNEAMSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIE6</accession>
    <name>C5DIE6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Very-long-chain 3-oxoacyl-CoA reductase</fullName>
            <ecNumber evidence="12">1.1.1.330</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">3-ketoacyl-CoA reductase</fullName>
            <shortName evidence="12">3-ketoreductase</shortName>
            <shortName evidence="12">KAR</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Microsomal beta-keto-reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0E11902g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the microsomal membrane bound fatty acid elongation system, which produces the 26-carbon very long-chain fatty acids (VLCFA) from palmitate. Catalyzes the reduction of the 3-ketoacyl-CoA intermediate that is formed in each cycle of fatty acid elongation. VLCFAs serve as precursors for ceramide and sphingolipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a very-long-chain (3R)-3-hydroxyacyl-CoA + NADP(+) = a very-long-chain 3-oxoacyl-CoA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48680"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:85440"/>
            <dbReference type="ChEBI" id="CHEBI:90725"/>
            <dbReference type="EC" id="1.1.1.330"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Endoplasmic reticulum membrane</location>
            <topology evidence="12">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.330" evidence="12"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553994.1">
        <property type="nucleotide sequence ID" value="XM_002553948.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIE6"/>
    <dbReference type="FunCoup" id="C5DIE6">
        <property type="interactions" value="732"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIE6"/>
    <dbReference type="GeneID" id="8292161"/>
    <dbReference type="KEGG" id="lth:KLTH0E11902g"/>
    <dbReference type="eggNOG" id="KOG1014">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_38_0_1"/>
    <dbReference type="InParanoid" id="C5DIE6"/>
    <dbReference type="OMA" id="LVAPGMM"/>
    <dbReference type="OrthoDB" id="5545019at2759"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045703">
        <property type="term" value="F:ketoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141040">
        <property type="term" value="F:very-long-chain 3-oxoacyl-CoA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030497">
        <property type="term" value="P:fatty acid elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05356">
        <property type="entry name" value="17beta-HSD1_like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000317">
        <property type="entry name" value="Very-long-chain 3-oxoacyl-CoA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03107">
        <property type="entry name" value="3_ketoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027533">
        <property type="entry name" value="3_ketoreductase_fungal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43086:SF2">
        <property type="entry name" value="HYDROXYSTEROID DEHYDROGENASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43086">
        <property type="entry name" value="VERY-LONG-CHAIN 3-OXOOACYL-COA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000126">
        <property type="entry name" value="11-beta-HSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11 12" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5 12" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 12" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9 12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0521">NADP</keyword>
    <keyword evidence="8 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="237"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR23557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="361" mass="39438" checksum="3DB9E3728BB7CE61" modified="2009-07-28" version="1">MAFPWHLSKKQRPTKAEEMSFTEQFLALGSNNKITNALLWGIFAVGVLKATTFVLSLSSLVADLFVLPGVSFSKYGAKKGKYCVVTGASDGIGKEYARQLAKRGFNLILISRTLSKLEALKADFESAFGVQVKVLAIDIAQDSPENYSKIRDICAGLPVTVLINNVGQSHSIPVPFLETEEDEMRNIITINTTATLKITQLVAPLIVQNAKESRCRGLILTMGSFGGLLPTPLLATYSGSKAFLQAWSAALAGELKPQNVDVEIVLSYLVTSAMSKVRRSSAMIPSPAQFVAATLKSVGRRSGAQERFATMTPYWSHALYHFLIENTVGVYSKAANTINYTFHKSIRTRALRKAARQAKHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIJ1</accession>
    <name>C5DIJ1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GDT1 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0E12914g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the GDT1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554039.1">
        <property type="nucleotide sequence ID" value="XM_002553993.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIJ1"/>
    <dbReference type="FunCoup" id="C5DIJ1">
        <property type="interactions" value="725"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIJ1"/>
    <dbReference type="GeneID" id="8292207"/>
    <dbReference type="KEGG" id="lth:KLTH0E12914g"/>
    <dbReference type="eggNOG" id="KOG2881">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040186_0_0_1"/>
    <dbReference type="InParanoid" id="C5DIJ1"/>
    <dbReference type="OMA" id="ILGHAIC"/>
    <dbReference type="OrthoDB" id="442680at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015085">
        <property type="term" value="F:calcium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032468">
        <property type="term" value="P:Golgi calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032472">
        <property type="term" value="P:Golgi calcium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001727">
        <property type="entry name" value="GDT1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049555">
        <property type="entry name" value="GDT1-like_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12608:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 165"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12608">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN HTP-1 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01169">
        <property type="entry name" value="GDT1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01214">
        <property type="entry name" value="UPF0016"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017495488" description="GDT1 family protein" evidence="6">
        <location>
            <begin position="24"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="39"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="103"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="184"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="222"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="255"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR23602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="280" mass="30404" checksum="6B8D0065CA25596B" modified="2009-07-28" version="1">MSLMKKCVLPLAIISTLATAAVAVQEKEGTDSAPDSPAKAFLFSASMIAVSEIGDKTFLIAALMAMRYPRLLVFTASASSLFLMTILSGLVGRTFTSWIPQSYTQFLAGILFLIFGYKLTLEGLAMSKDMGVEEELAEVEEEIAVQDLNKNMTDVEGGELARDSSKNFTKNTVATKFVKKVTDLGAYIFTPVWVQIFSMVFLGEFGDRSQISTIAMASGSDYWFVIWGAIVGHAFCTALAVVGGKMLATKISMRTVTLGGALSFFIFAVMYIREAFLNPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIJ8</accession>
    <name>C5DIJ8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">low-specificity L-threonine aldolase</fullName>
            <ecNumber evidence="8">4.1.2.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0E13090g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-allo-threonine = acetaldehyde + glycine</text>
            <dbReference type="Rhea" id="RHEA:26209"/>
            <dbReference type="ChEBI" id="CHEBI:15343"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:58585"/>
            <dbReference type="EC" id="4.1.2.48"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-threonine = acetaldehyde + glycine</text>
            <dbReference type="Rhea" id="RHEA:19625"/>
            <dbReference type="ChEBI" id="CHEBI:15343"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="EC" id="4.1.2.48"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid degradation; L-threonine degradation via aldolase pathway; acetaldehyde and glycine from L-threonine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the threonine aldolase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.48" evidence="8"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554046.1">
        <property type="nucleotide sequence ID" value="XM_002554000.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIJ8"/>
    <dbReference type="STRING" id="559295.C5DIJ8"/>
    <dbReference type="GeneID" id="8292215"/>
    <dbReference type="KEGG" id="lth:KLTH0E13090g"/>
    <dbReference type="eggNOG" id="KOG1368">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029381_1_0_1"/>
    <dbReference type="InParanoid" id="C5DIJ8"/>
    <dbReference type="OMA" id="VQTNIVI"/>
    <dbReference type="OrthoDB" id="10261951at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008732">
        <property type="term" value="F:L-allo-threonine aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006545">
        <property type="term" value="P:glycine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006567">
        <property type="term" value="P:L-threonine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000030">
        <property type="entry name" value="Low-specificity L-threonine aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001597">
        <property type="entry name" value="ArAA_b-elim_lyase/Thr_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023603">
        <property type="entry name" value="Low_specificity_L-TA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48097:SF9">
        <property type="entry name" value="L-THREONINE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48097">
        <property type="entry name" value="L-THREONINE ALDOLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01212">
        <property type="entry name" value="Beta_elim_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017617">
        <property type="entry name" value="Thr_aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aromatic amino acid beta-eliminating lyase/threonine aldolase" evidence="10">
        <location>
            <begin position="13"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="9">
        <location>
            <position position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00050410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00050939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00060555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00066573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR017617-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR23609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="384" mass="42841" checksum="790657385988B375" modified="2009-07-28" version="1">MPDLPQYTTSYNDFRSDTFTIPTPDMFAAGANASVGDVIFQEDACTCLLEQRVSQELLGGKLYGGSEAQGLFCMSTTMSNQIAIRAHLNFAPPFSILCDKRAHVYVDECAGIAVLSQALVVPVAASNDRFLTLEDIQEAYIPDDGDIHLAPTRLICLENTLHGMCFPYEELKRISDWCHEEGIKLHLDGARFWNAACSSGEDPVGYMRKVGPLFDSLSLCFSKSLGAPIGSMLVGGPEFRARARHLLKQQGGGIRQAGFITKIAAYCLDRNFPQAVAEVSSKTLEFWEDLYTTLKDKYAMELRLAHPVETNFIFIDLASSGINMSKFLEIGERNNVRLFDGRLAFHYQNIKPEGLALLKKTFTDVAEYYQKNDYKPEGRSTRIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIN2</accession>
    <name>C5DIN2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">KLTH0E13838p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0E13838g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554080.1">
        <property type="nucleotide sequence ID" value="XM_002554034.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIN2"/>
    <dbReference type="FunCoup" id="C5DIN2">
        <property type="interactions" value="525"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIN2"/>
    <dbReference type="GeneID" id="8292249"/>
    <dbReference type="KEGG" id="lth:KLTH0E13838g"/>
    <dbReference type="eggNOG" id="KOG0072">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040729_9_3_1"/>
    <dbReference type="InParanoid" id="C5DIN2"/>
    <dbReference type="OMA" id="VRWSKDQ"/>
    <dbReference type="OrthoDB" id="2011769at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04151">
        <property type="entry name" value="Arl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000510">
        <property type="entry name" value="ADP-ribosylation factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 11 13" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0519">Myristate</keyword>
    <keyword evidence="5 11 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="25"/>
            <end position="32"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="127"/>
            <end position="130"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR23643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="183" mass="20647" checksum="F47274FFBDB486E8" modified="2009-07-28" version="1">MGNFFSSMFDRLWGVNREMRILILGLDGAGKTTILYRLQIGEVVTTKPTIGFNVETLVYKNLKLNVWDLGGQTSIRPYWRCYYSNTAAVIFVVDSTDRDRMPTAAHELHLMLREDELQDAALLVFANKQDQPAALSASEVSRELNLVELKDRSWSIVASSATNGDGISEGLDWLIDVVREEQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIS1</accession>
    <name>C5DIS1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0E14718p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0E14718g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL16 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554119.1">
        <property type="nucleotide sequence ID" value="XM_002554073.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIS1"/>
    <dbReference type="FunCoup" id="C5DIS1">
        <property type="interactions" value="318"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIS1"/>
    <dbReference type="GeneID" id="8292289"/>
    <dbReference type="KEGG" id="lth:KLTH0E14718g"/>
    <dbReference type="eggNOG" id="KOG3422">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078858_0_1_1"/>
    <dbReference type="InParanoid" id="C5DIS1"/>
    <dbReference type="OMA" id="KGAVEYW"/>
    <dbReference type="OrthoDB" id="268521at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01433">
        <property type="entry name" value="Ribosomal_L16_L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1170.10:FF:000010">
        <property type="entry name" value="60S ribosomal protein L16, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.10">
        <property type="entry name" value="Ribosomal protein L10e/L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047873">
        <property type="entry name" value="Ribosomal_uL16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000114">
        <property type="entry name" value="Ribosomal_uL16_bact-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020798">
        <property type="entry name" value="Ribosomal_uL16_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016180">
        <property type="entry name" value="Ribosomal_uL16_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036920">
        <property type="entry name" value="Ribosomal_uL16_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01164">
        <property type="entry name" value="rplP_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12220">
        <property type="entry name" value="50S/60S RIBOSOMAL PROTEIN L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12220:SF13">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL16M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00252">
        <property type="entry name" value="Ribosomal_L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00060">
        <property type="entry name" value="RIBOSOMALL16"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54686">
        <property type="entry name" value="Ribosomal protein L16p/L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00701">
        <property type="entry name" value="RIBOSOMAL_L16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR23682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="217" mass="25004" checksum="AAF88EAF3055EA6C" modified="2009-07-28" version="1">MFGSLLKPTRLTASPFNVLKRFTHEYAPRFKHVSKKQKGRVPVRVGGSIKGSTLQFGRFGLRLRSEGERLSAQQLREADNAIMRYVRPLTNGKMWRRLCTNIAVCIKGNETRMGKGKGGFDHWMARVPTGKIIFEMDGDDLHEKVAREAFRKAGTKLPGVYEFVSRDSLVRVGLKSFKDPKDDPKINYLEELKKKPTKDFINEQRAKQPQYRMFRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIU3</accession>
    <name>C5DIU3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">KLTH0E15224p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0E15224g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554141.1">
        <property type="nucleotide sequence ID" value="XM_002554095.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIU3"/>
    <dbReference type="FunCoup" id="C5DIU3">
        <property type="interactions" value="218"/>
    </dbReference>
    <dbReference type="GeneID" id="8292312"/>
    <dbReference type="KEGG" id="lth:KLTH0E15224g"/>
    <dbReference type="eggNOG" id="KOG0758">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015166_16_2_1"/>
    <dbReference type="InParanoid" id="C5DIU3"/>
    <dbReference type="OMA" id="HCITETV"/>
    <dbReference type="OrthoDB" id="409586at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000064">
        <property type="term" value="F:L-ornithine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990575">
        <property type="term" value="P:mitochondrial L-ornithine transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000163">
        <property type="entry name" value="Mitochondrial carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624:SF51">
        <property type="entry name" value="CARRIER PROTEIN YMC2, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="24"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="119"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="213"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR23704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="303" mass="32872" checksum="E4C6BB6B614A036E" modified="2009-07-28" version="1">MSEELGPPQLIDELNTPHDSSRVFKDLLAGTAGGIAQVLVGQPFDTTKVRLQTSSTPTTAVQVVKNLIKNEGLRGFYKGTLTPLVGVGACVSCQFGVNEAMKRFFRGPNSDVNKPLTLPQYYVCGLAGGIANSFLASPIEHVRIRLQTQTSSGTAASFKGPLDCIKKLKSNGALMRGLTPTVLREAQGCATYFLTYEALVANEVQKGLKRSDVPAWKLCMFGAISGVTLWLMVYPIDVVKSIMQTDNLQSPVHGRDPIVVAKKMYTQRGWRVFFKGFGPTMLRAAPANGATFATFELTMRFLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIV7</accession>
    <name>C5DIV7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0E15532p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0E15532g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554155.1">
        <property type="nucleotide sequence ID" value="XM_002554109.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIV7"/>
    <dbReference type="FunCoup" id="C5DIV7">
        <property type="interactions" value="518"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIV7"/>
    <dbReference type="GeneID" id="8292326"/>
    <dbReference type="KEGG" id="lth:KLTH0E15532g"/>
    <dbReference type="eggNOG" id="KOG1577">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_0_0_1"/>
    <dbReference type="InParanoid" id="C5DIV7"/>
    <dbReference type="OMA" id="CASIKPV"/>
    <dbReference type="OrthoDB" id="416253at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000002">
        <property type="entry name" value="2,5-diketo-D-gluconic acid reductase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11732">
        <property type="entry name" value="ALDO/KETO REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00798">
        <property type="entry name" value="ALDOKETO_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00063">
        <property type="entry name" value="ALDOKETO_REDUCTASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="5">
        <location>
            <begin position="22"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="4">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR23718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="309" mass="34526" checksum="08982CC9DC804B6E" modified="2009-07-28" version="1">MSGPTNSSAVGKLRTGATIPLLGFGTWRSTEEDGYNAVLTALKVGYRHIDTAAVYGNEAAVGRAIRDSGVPRDQLFVTTKLWNTQHRDPRLALDQSLQRLGLDYVDLYLIHWPLPLRTQRITDGNLFTVPTKEDGKPDVDEQWSYVKTWEMMQDLPDTSKTRAVGVSNFSVTQLQNLLAASGTKVIPAVNQVETHPLLPQDKLYKFCSENNILLEAYSPLGSQGSPLVEEPAVKEIASKHNADPAQVLISWGIKRGYVILPKSVTPSRIESNFKIVDLTDDDFAKINNIHKEKGEKRINDPDWFHFDEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIV8</accession>
    <name>C5DIV8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">T-complex protein 1 subunit eta</fullName>
            <shortName evidence="10">TCP-1-eta</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">CCT-eta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0E15554g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. Known to play a role, in vitro, in the folding of actin and tubulin. In yeast may play a role in mitotic spindle formation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Heterooligomeric complex that forms two stacked rings.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554156.1">
        <property type="nucleotide sequence ID" value="XM_002554110.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIV8"/>
    <dbReference type="FunCoup" id="C5DIV8">
        <property type="interactions" value="1548"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIV8"/>
    <dbReference type="GeneID" id="8292327"/>
    <dbReference type="KEGG" id="lth:KLTH0E15554g"/>
    <dbReference type="eggNOG" id="KOG0361">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008891_7_3_1"/>
    <dbReference type="InParanoid" id="C5DIV8"/>
    <dbReference type="OMA" id="HRKGNTW"/>
    <dbReference type="OrthoDB" id="10248520at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03340">
        <property type="entry name" value="TCP1_eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000074">
        <property type="entry name" value="T-complex protein 1 subunit delta, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000017">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.260.10:FF:000022">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000006">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012720">
        <property type="entry name" value="Chap_CCT_eta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02345">
        <property type="entry name" value="chap_CCT_eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="527"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="530"/>
            <end position="548"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR23719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="548" mass="59325" checksum="E4A4347868518124" modified="2009-07-28" version="1">MNFGNQTPTIVVLKEGTDASQGRGQIISNINACLAIQDTLRPTLGPLGSDILIVSSSGKTTISNDGATILKLLDVVHPAAKTLVDISRAQDAEVGDGTTSVTILAGELMKEAKPFLEEGISSHVIVKGYRKASQLAVAKIQELAQTVTNNGNDRDLLERCARTAMSSKLISSNADFFVKMCVDAVLSLDQEELDDKLIGIKKVPGGAMEDSLFVNGVAFQKTFSYAGFEQQPKKFKSPKILSLNVELELKAEKDNAEVRVEHVEDYHAVVDAEWQIIMDKLKQIEDSGANIVLSKLPIGDLATQYFADRNIFCAGRVAAEDMNRVILAVGGAVQSTTSDIRAEHLGTCEMFEEVQVGSERYNIFKGCPQARTCTLLLRGGAEQVIAEVERSLHDAIMVVKRAIKSKSVVPGGGAIEMEVSKYLRDWSKTIAGKQQLIMHAYAKALEVIPRQLCENAGLDATEILNKLRMAHSRGEQGYGVDFDAETVGDNFAKFVWEPALVKINALNSATEATNLILSVDETIKNKESQPANAGMPAPGARGMGMPMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJB0</accession>
    <name>C5DJB0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0F14960p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0F14960g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the actin family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554836.1">
        <property type="nucleotide sequence ID" value="XM_002554790.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJB0"/>
    <dbReference type="FunCoup" id="C5DJB0">
        <property type="interactions" value="975"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJB0"/>
    <dbReference type="GeneID" id="8293065"/>
    <dbReference type="KEGG" id="lth:KLTH0F14960g"/>
    <dbReference type="eggNOG" id="KOG0797">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006974_0_1_1"/>
    <dbReference type="InParanoid" id="C5DJB0"/>
    <dbReference type="OMA" id="AYKCMWA"/>
    <dbReference type="OrthoDB" id="5572108at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="CDD" id="cd10206">
        <property type="entry name" value="ASKHA_NBD_Arp8-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="140"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="32"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="151"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR24399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="775" mass="87511" checksum="86C133C18B32DBEA" modified="2009-07-28" version="1">MTSSQALEPPSEDVPMGDAEDAEMEHEGDYSTADQTPLSMTPALSSNPNASKVKKPDPHAKQQTKVPGHLLEKRRLGRQKAAEQYAQKMKTIGIEKRDNPLLHQLGLFKPLSLINQKNYSSDYLKRDDQIFAMRERKSLRNANANQATPDMPEDDDQESVDGESDAQTIVIHPGSRNLKIGLATEVLPQSIPCCVAVPKRPRTSDSTPEPQVDEEEYISAKESIQQDFKERMRYYKRKVIPNSHDIAVNFNARVTPEAIPEHNDLHRVDWIQDASKCYYGKDATRCLPSHFTVRHPFWKGRFNIESPDYGSLQELLVDVSELLKFALSQPKIDVRPSQIPNYKVVIVVPDAFDKAYVETFIRLLITDLKFQAVAVIQESLASCYGAGIGDSTCVVDIGATTTKIACVDEGLVIDNSVITLDYGGDDITKLFAKFLLESNFPYQDLDLNSPEGWSLMEQLKEKYATFQDADVTIQLYNFIKRIPNKPGAEKFEFKVFDEVMTAPMGLFFPHILRLLKDKNVPVNKYVTSQLPRSKDMFTCKDNNPKSVSQLACQKKTTYCDMHRDLDILQKLLNLPSEIEEYQSSTCANLEPAYTPLEKAIVESITNACVSLDNNHSKAANFYSNILVVGGSSKFSSLDFILTDRINIWRPRLLSVNTLPTFQNQVSKQVKEFEQTQKLSEIKDEEELAAQKKKLAKTIKVELQSYWEGVDALGGNESVFPANVLPAPREMDPALLTWKGGSVFARLKLIEELYVSESDWDILGSRILQYKCIFDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJL4</accession>
    <name>C5DJL4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0F17380p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0F17380g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554940.1">
        <property type="nucleotide sequence ID" value="XM_002554894.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJL4"/>
    <dbReference type="FunCoup" id="C5DJL4">
        <property type="interactions" value="218"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJL4"/>
    <dbReference type="GeneID" id="8293174"/>
    <dbReference type="KEGG" id="lth:KLTH0F17380g"/>
    <dbReference type="eggNOG" id="KOG4302">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_333226_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJL4"/>
    <dbReference type="OMA" id="KRITRHF"/>
    <dbReference type="OrthoDB" id="642895at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990023">
        <property type="term" value="C:mitotic spindle midzone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051256">
        <property type="term" value="P:mitotic spindle midzone assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007145">
        <property type="entry name" value="MAP65_Ase1_PRC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19321:SF41">
        <property type="entry name" value="FASCETTO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19321">
        <property type="entry name" value="PROTEIN REGULATOR OF CYTOKINESIS 1 PRC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03999">
        <property type="entry name" value="MAP65_ASE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="637"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="723"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="499"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="723"/>
            <end position="732"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR24503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="907" mass="103219" checksum="A2FD0F25D5EA4666" modified="2009-07-28" version="1">MSDLQNLNETHVPKCFYSQDNHSSSQVSSTAASSVRSPMLFANYSESTEFLRLTPVNIKNLTSPIRAPRELAQMGREESASFTAYKENFNLITRKLEKLLDELNVIYKEIGYSNTQIASKEKLIFNNISDSITGFFEQANQEREKITSENQICQQVLQRILQVTNDSKGTSTIPDLYTRNVIILGDAQVSNNGNPSSPKKPTSLLNKQKTLKKAKGYVIKTYIPKLSSFLDSVMKLKSLVNTVSDYQPEEQEWDVEELLSVIPPLETCKLYKSFLTSSVHDVDKIYDFIVQNRQALLSTINFNDISEKMVGRVDTLSSLFEKEFRSRMDKLHETGKGILDVLTILGLTPEGVLEDSVARALRQVSAESSQNTEDMCFSTNALTKLEEVQKHFKEIEQSRKQAKEKLQKNCSQLWEKLKIPSSYINKFESDNGNLSDMALKNFTEELDRLQEMKKKLIKLLIEDSWAKIQQLWLAMHFTDRETSAFKEMYASMTARSATLEDDEKLLETCEAEIVDLEKRFSLYRPILKLIEEFKSLQNDKVALEKSSKDSSRLLLRNSHRILLEEEKTRKRITRHFPDVIQDLIRKLIKFEEDSGGPLMIEGSRFLDVVLEQEEEIISKYPRSRVNSFFKGPTVSYNNSSKTAATRRSPVKDFTQQRKVPKTFRRSTERTPMRTSKPGPILGPSDNSTNQSFNRYMRATRMSSPIRELDHDMRAVVNMRSSPTKIPTLNRSATFPRESGMPGTSRAPKGTTINSTRLMQISPNKLNRVESNLGKTRHIRKAPMNLSQNSLGAKLEESGASIDKENNVLFMLKSPAKIKSNSVLAQQPPSKEQDDSVYRITKSPEGKFTLNVEQNDADNGFGEGEDTSIMADDENFAKWKTERLAKMNATQGDMGDAPSCINWDTDVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJS1</accession>
    <name>C5DJS1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">phosphoadenylyl-sulfate reductase (thioredoxin)</fullName>
            <ecNumber evidence="3">1.8.4.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">3'-phosphoadenylylsulfate reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">PAPS reductase, thioredoxin dependent</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">PAdoPS reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0F18656g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>[thioredoxin]-disulfide + sulfite + adenosine 3',5'-bisphosphate + 2 H(+) = [thioredoxin]-dithiol + 3'-phosphoadenylyl sulfate</text>
            <dbReference type="Rhea" id="RHEA:11724"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17359"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="EC" id="1.8.4.8"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PAPS reductase family. CysH subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.8.4.8" evidence="3"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554997.1">
        <property type="nucleotide sequence ID" value="XM_002554951.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJS1"/>
    <dbReference type="FunCoup" id="C5DJS1">
        <property type="interactions" value="699"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJS1"/>
    <dbReference type="GeneID" id="8293232"/>
    <dbReference type="KEGG" id="lth:KLTH0F18656g"/>
    <dbReference type="eggNOG" id="KOG0189">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044089_0_1_1"/>
    <dbReference type="InParanoid" id="C5DJS1"/>
    <dbReference type="OMA" id="PIARWTQ"/>
    <dbReference type="OrthoDB" id="7869097at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004604">
        <property type="term" value="F:phosphoadenylyl-sulfate reductase (thioredoxin) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019344">
        <property type="term" value="P:cysteine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019379">
        <property type="term" value="P:sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23945">
        <property type="entry name" value="PAPS_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000151">
        <property type="entry name" value="Phosphoadenosine phosphosulfate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00063">
        <property type="entry name" value="CysH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004511">
        <property type="entry name" value="PAPS/APS_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002500">
        <property type="entry name" value="PAPS_reduct_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011800">
        <property type="entry name" value="PAPS_reductase_CysH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00434">
        <property type="entry name" value="cysH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02057">
        <property type="entry name" value="PAPS_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002537">
        <property type="entry name" value="PRK02090.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46509">
        <property type="entry name" value="PHOSPHOADENOSINE PHOSPHOSULFATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46509:SF1">
        <property type="entry name" value="PHOSPHOADENOSINE PHOSPHOSULFATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01507">
        <property type="entry name" value="PAPS_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000857">
        <property type="entry name" value="PAPS_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0198">Cysteine biosynthesis</keyword>
    <keyword evidence="7" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphoadenosine phosphosulphate reductase" evidence="13">
        <location>
            <begin position="45"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00052536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00078053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00082472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00082553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR24560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="261" mass="29906" checksum="CC809EACFD9DF611" modified="2009-07-28" version="1">MVKSYQLNNEIVVTQEQVDHWNAALAQMQSPRQILEWALATFPHLFQTTAFGLTGLATIDMLSKIQDQSITPLIFIDTLHHFPQTLDLLRNIEDRYYKPRNQTINVYKPEGANNESEFAAKHGDFLWERDETKYDFLVKVEPAHRAYKELGVTAVFTGRRKSQGGSRSSLLFVELDELNGILKINPLANWDFQQVKAYIDNHQVPYNELLDLGYKSIGDYHSTQPVADGEDERAGRWKGKSKTECGIHETSRFAQFLGAKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKE2</accession>
    <name>C5DKE2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0F03938p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0F03938g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MNN1/MNT family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554380.1">
        <property type="nucleotide sequence ID" value="XM_002554334.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKE2"/>
    <dbReference type="FunCoup" id="C5DKE2">
        <property type="interactions" value="59"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKE2"/>
    <dbReference type="GeneID" id="8292574"/>
    <dbReference type="KEGG" id="lth:KLTH0F03938g"/>
    <dbReference type="eggNOG" id="ENOG502QTWU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013298_1_1_1"/>
    <dbReference type="InParanoid" id="C5DKE2"/>
    <dbReference type="OMA" id="IMFIHAS"/>
    <dbReference type="OrthoDB" id="430354at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046354">
        <property type="term" value="P:mannan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000177">
        <property type="entry name" value="MNN5p Alpha-1,2-mannosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022751">
        <property type="entry name" value="Alpha_mannosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31646">
        <property type="entry name" value="ALPHA-1,2-MANNOSYLTRANSFERASE MNN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31646:SF6">
        <property type="entry name" value="ALPHA-1,2-MANNOSYLTRANSFERASE MNN5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11051">
        <property type="entry name" value="Mannosyl_trans3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950514" evidence="7">
        <location>
            <begin position="20"/>
            <end position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR23943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="562" mass="64607" checksum="23BA94E3DC026BB6" modified="2009-07-28" version="1">MHIWRRKVVLCCCVLLALAYALFAATARKSLDTPSLRERVYSDVFDALLQYRPVRPPKDRQAAVRQAGCEMKGVGVNRGDAVDAMLYENLDRCYRPTKAQFEGLQAPHSAFLTQLRTTFAYEADSPVFERLFPRERGFVTVGGGRFSLLAYSMLRALRARGSKLPAEVVIPPQDAAGEEHFCNEVLPELNARCVFFEDALPRALVETWDFERYQIKAVALLLSSFKKIVFIDADDFPIQNLDSLFDSEVLREHGLVLWPDLWRRATAPVFYRLANIDVDLRRRVRNMGDNLSPASRYTDPDQDPEQLQKIAPFHDFAGTVPDPTTESGQMVIDKVRHFQTLLLALYYNVYGPEWYYSLFSQGTAGEGDKETFATAAHVLGLPFYQVKNKLAFDGYFHPNGDGFRGVALYQHDCVEDYLLYCRARDIIKENQDSFSAYKPDYDPEPDFYKSLMAPEGHPEIPVMFAHASFHKFDPWQLYHEKVYLDNDGAHFRSFHNIKRIGNFDIELFVFEGLQKAICSDNPIRFKYLEDKFIHADWPKVCQYVEDRVKYLQDTHDPAVGLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKL8</accession>
    <name>C5DKL8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0F05764p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0F05764g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554456.1">
        <property type="nucleotide sequence ID" value="XM_002554410.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKL8"/>
    <dbReference type="GeneID" id="8292654"/>
    <dbReference type="KEGG" id="lth:KLTH0F05764g"/>
    <dbReference type="HOGENOM" id="CLU_2413637_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKL8"/>
    <dbReference type="OrthoDB" id="4052953at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR24019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="92" mass="9902" checksum="B8797651F80C50AC" modified="2009-07-28" version="1">MLRLPQATTLVKSAPALMPTARNSAPSVSRIARRLHSCDDACVPNDAENAGTGARVLRNPVAGNTFSSFKEYREIARTYGPIHASRGSHPSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DL34</accession>
    <name>C5DL34_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="16">KLTH0F09614p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">KLTH0F09614g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Components of the endosome-vacuole trafficking pathway that regulates nutrient transport. May be involved in processes which determine whether plasma membrane proteins are degraded or routed to the plasma membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endosome membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SSH4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554622.1">
        <property type="nucleotide sequence ID" value="XM_002554576.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DL34"/>
    <dbReference type="FunCoup" id="C5DL34">
        <property type="interactions" value="69"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DL34"/>
    <dbReference type="GeneID" id="8292829"/>
    <dbReference type="KEGG" id="lth:KLTH0F09614g"/>
    <dbReference type="eggNOG" id="KOG1477">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016552_3_0_1"/>
    <dbReference type="InParanoid" id="C5DL34"/>
    <dbReference type="OMA" id="SPYPYFR"/>
    <dbReference type="OrthoDB" id="258495at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12910">
        <property type="entry name" value="SPRY_SSH4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000065">
        <property type="entry name" value="Ear1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035780">
        <property type="entry name" value="SPRY_Ssh4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050618">
        <property type="entry name" value="Ubq-SigPath_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12864">
        <property type="entry name" value="RAN BINDING PROTEIN 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0967">Endosome</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="5" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="56"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="15">
        <location>
            <begin position="159"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00025244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAR24185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="505" mass="56824" checksum="E2DBE811D22F4742" modified="2009-07-28" version="1">MFLAELLRKHLGVVLLGALWVRPSACGYVPERFEEQHYLGSEPYDDGYKNDLDIDLMLFSLAFMVSCYFVLTLVYFAVKFFLRRYFRSGIALSGDGEGHVIGPAVNARWPSPLDDVNAVRDKLAAMSPEEQFYYKQGEEFIKQNPPLLIPDQLPGGSNTVDPIINEHTLRFIDEEGAHAWEFQPDPKLPNDSVLVENKTEITFLNYNYDVSVMTNLPIPRLNWVYYCEFKIFELNTAGSNNNNHLSQNELISFGLSTSPYPYFRLPGRHHHSVAYDSTGARRFNDSFELSAELSSIFPSCEKGDVIGIGYRTNSGTIFFTRNGKKLSEKSIGGHIKGWKFKYIYPVVGSNIPCKIHVNFGTYGFVYIEANVKKWGYAKSHGLKLPPPSYEDYGQDVLLESSYEDDATDNESISTTDGDIIDNEGHLLPPPPGFEFSVSPQPSDAGQFTLNSLPAEPPVYASDNQIDGEDNETAERLEHIVRDSDIRDSAYPGNELGKNTMANQFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DL74</accession>
    <name>C5DL74_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chitobiosyldiphosphodolichol beta-mannosyltransferase</fullName>
            <ecNumber evidence="3">2.4.1.142</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Asparagine-linked glycosylation protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Beta-1,4-mannosyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">GDP-Man:GlcNAc2-PP-dolichol mannosyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">GDP-mannose-dolichol diphosphochitobiose mannosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ordered locus">KLTH0F10604g</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Participates in the formation of the lipid-linked precursor oligosaccharide for N-glycosylation. Involved in assembling the dolichol-pyrophosphate-GlcNAc(2)-Man(5) intermediate on the cytoplasmic surface of the ER.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>an N,N'-diacetylchitobiosyl-diphospho-di-trans,poly-cis-dolichol + GDP-alpha-D-mannose = a beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13865"/>
            <dbReference type="Rhea" id="RHEA-COMP:19510"/>
            <dbReference type="Rhea" id="RHEA-COMP:19511"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57269"/>
            <dbReference type="ChEBI" id="CHEBI:57527"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:58472"/>
            <dbReference type="EC" id="2.4.1.142"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:13866"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.4.1.142" evidence="3"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554662.1">
        <property type="nucleotide sequence ID" value="XM_002554616.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DL74"/>
    <dbReference type="FunCoup" id="C5DL74">
        <property type="interactions" value="945"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DL74"/>
    <dbReference type="GeneID" id="8292874"/>
    <dbReference type="KEGG" id="lth:KLTH0F10604g"/>
    <dbReference type="eggNOG" id="KOG2941">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012079_0_0_1"/>
    <dbReference type="InParanoid" id="C5DL74"/>
    <dbReference type="OMA" id="CKLIIDW"/>
    <dbReference type="OrthoDB" id="614844at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004578">
        <property type="term" value="F:chitobiosyldiphosphodolichol beta-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000216">
        <property type="entry name" value="Chitobiosyldiphosphodolichol beta-mannosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026051">
        <property type="entry name" value="ALG1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13036">
        <property type="entry name" value="BETA1,4 MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13036:SF0">
        <property type="entry name" value="CHITOBIOSYLDIPHOSPHODOLICHOL BETA-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="177"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="19">
        <location>
            <begin position="252"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00031434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00031566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00033088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00045071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CAR24225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="447" mass="51517" checksum="6FC7F2063916E4B4" modified="2009-07-28" version="1">MFEGLPQWVWWLLTVYASLPLVCYLTVPFLFYGNRSTKKRIIICVLGDLGHSPRMCYHARSFSQQGWQVELCGYLEEQPPSDILEDTNITIHQLPAFQGRKGGSFLIKAVRKVSLQIYAILRLLWRLRGSDYFLLQNPPSIPILPMAAVYCTFSRCKLIIDWHNFGYSILKLKLGSFWHPLVLISFAVEYIFAKFAAYNLTVTRAMKDYLIHTFGLSKKRVAVLYDRPAFQFRPLKADRQAALQQDFIKPFIPGDFNISKGDRIIVTSTSFTPDEDLSILIGALKIYENSCEKFDDKLPKILCFVTGKGPLKQHFIDKVAETKWDRVHIEFLWLSNEDYPKLLQLCDYGVSLHTSSSGLDLPMKILDMFGSGIPVVSYNYPVLNELVQHNVNGLKFLDRRELHEALIFIMKDSHVSEVLKAGALKESNNRWQDSWEKAMEEISVIRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLF9</accession>
    <name>C5DLF9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0G00418p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0G00418g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the trans-sulfuration enzymes family. MET7 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555057.1">
        <property type="nucleotide sequence ID" value="XM_002555011.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLF9"/>
    <dbReference type="FunCoup" id="C5DLF9">
        <property type="interactions" value="157"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLF9"/>
    <dbReference type="GeneID" id="8293315"/>
    <dbReference type="KEGG" id="lth:KLTH0G00418g"/>
    <dbReference type="eggNOG" id="KOG0053">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011302_1_0_1"/>
    <dbReference type="InParanoid" id="C5DLF9"/>
    <dbReference type="OMA" id="LACPYVH"/>
    <dbReference type="OrthoDB" id="10047078at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003962">
        <property type="term" value="F:cystathionine gamma-synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000063">
        <property type="entry name" value="Probable cystathionine gamma-synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051750">
        <property type="entry name" value="Trans-sulfuration_enzymes"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42699">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42699:SF1">
        <property type="entry name" value="CYSTATHIONINE GAMMA-SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="3 7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR24620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="568" mass="63843" checksum="8CBE2B1F099D0E25" modified="2009-07-28" version="1">MTEIEFGQPLPSNLEYAVSFGIPTWESAIGYVEKNPDYISKMVTGYPRYFPQPAIQKLCDHFLQKFGEGSEGCRPFPSAKSASNCLEYVKSVSGPQSKARMEVETFYLNDNGKDAGEDSYQLAITIAAIFASEDELETVKEYWKLTGECVSSRLAVFVNKFLEKLPADSKLSQNETGTDIQLLKRTGEEAKVLLRNRIANNHCNPFGEKREVVDKRPILDATKDVYLVSSGMSAVFTARKLLTHWEKNKNRKPLSHKNRDFHDKQHPVCETAAVFGFPFKDTKVIMEKFGKCQFFGFGDSRDLAGLRSLLKGGKERILGVFLETPSNPMLNMPDLKQLRNMANEYGFFIVVDDTIGGLNVDVLPYADIVCTSLTKLFSGSSNVMGGSIILNPKSALYSCALQYFRGGEFEELLWLEDAIILEANSRDFEERTTRTNENTMKLIQEVLLPEEGKIFKKVYYPTVSSKETFKNFESVRTNGGGYGSLFSLSFFNEEDAKKFYNSLTVFKGPSNGTNFTLACPYVHLAHHFELEEVSTFGAHSSLVRVSVGLENTQWLLNAFSSVINLLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLM6</accession>
    <name>C5DLM6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Clathrin heavy chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0G01892g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Clathrin is the major protein of the polyhedral coat of coated pits and vesicles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasmic vesicle membrane</location>
            <topology evidence="6">Peripheral membrane protein</topology>
            <orientation evidence="6">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <location evidence="6">Coated pit</location>
            <topology evidence="6">Peripheral membrane protein</topology>
            <orientation evidence="6">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the clathrin heavy chain family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555124.1">
        <property type="nucleotide sequence ID" value="XM_002555078.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DLM6">
        <property type="interactions" value="1371"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLM6"/>
    <dbReference type="GeneID" id="8293382"/>
    <dbReference type="KEGG" id="lth:KLTH0G01892g"/>
    <dbReference type="eggNOG" id="KOG0985">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002136_0_0_1"/>
    <dbReference type="InParanoid" id="C5DLM6"/>
    <dbReference type="OMA" id="HCYDLLH"/>
    <dbReference type="OrthoDB" id="2113814at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030132">
        <property type="term" value="C:clathrin coat of coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030130">
        <property type="term" value="C:clathrin coat of trans-Golgi network vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071439">
        <property type="term" value="C:clathrin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032051">
        <property type="term" value="F:clathrin light chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006898">
        <property type="term" value="P:receptor-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000001">
        <property type="entry name" value="Clathrin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000002">
        <property type="entry name" value="Clathrin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000082">
        <property type="entry name" value="Clathrin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.110:FF:000003">
        <property type="entry name" value="Clathrin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.110">
        <property type="entry name" value="Clathrin heavy-chain terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055358">
        <property type="entry name" value="CHCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000547">
        <property type="entry name" value="Clathrin_H-chain/VPS_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015348">
        <property type="entry name" value="Clathrin_H-chain_linker_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016025">
        <property type="entry name" value="Clathrin_H-chain_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016341">
        <property type="entry name" value="Clathrin_heavy_chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10292:SF1">
        <property type="entry name" value="CLATHRIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10292">
        <property type="entry name" value="CLATHRIN HEAVY CHAIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00637">
        <property type="entry name" value="Clathrin"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09268">
        <property type="entry name" value="Clathrin-link"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13838">
        <property type="entry name" value="Clathrin_H_link"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002290">
        <property type="entry name" value="Clathrin_H_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00299">
        <property type="entry name" value="CLH"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50989">
        <property type="entry name" value="Clathrin heavy-chain terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50236">
        <property type="entry name" value="CHCR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0168">Coated pit</keyword>
    <keyword evidence="5 6" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="3 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Clathrin heavy chain linker core motif" evidence="8">
        <location>
            <begin position="336"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="541"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="690"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="837"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="983"/>
            <end position="1128"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="1132"/>
            <end position="1273"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="1278"/>
            <end position="1424"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="1427"/>
            <end position="1570"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR002290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF09268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR24687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1657" mass="186964" checksum="0D42E0FE992923DA" modified="2009-07-28" version="1">MSDLPIEFTELVDLTSIGISPQSLDFRSTTFESDRFVTARESQNGANSVAIVDLQNGNQVTRKNMGGDSAIMHPSQMVISVRANGTIVQIFNLETKTKLKSFTLEEPVLFWKWLSEETLGFVTANSLYVSNVFDGDTSRKPSKLTQRHANLNNAQIINFVANKRLDWFAVVGIIQENGRVAGKIQLFSKARGISQAIEGHVATFSTILLEGNTQPVQVFVTGNKTASGTGELRIIEIDHDASNAVSYQKKTVDIFFPPDAVNDFPISVQVSEKYGVIYVLTKHGFIHLYELETGANLFVNRITVEPVFTATTYNQKNGIACINRRGQVLAVEISTDQIVPYVLNKLSNVSLALKMASRGGLPGADDLFNKQFDTLLASGDYQNAAKVAASSQQLRTQTTINRLKNIQAPPGAISPILLYFSTLLDKGKLNKYETIELARPVLQQDRKQLFEKWLKEDKLECSEELGDVVKPFDTTLALACYLRAEAHSKVVTCLAELQQFDKILPYAQKVGYQPNFIVLISNLARSSPDKASEFAVSLLQSPETASQIDIEKIADIFFAQNFVQQGTSFLLDALKGDTPDQGHLQTRVLEVNLLHAPQVADAIMGNSIFSHYDKPTIASLAEKAGLYQRALENYTDIKDIKRCIVHSNALPIDWLVAYFGKLNVEQSVACLRALLDDNLQANVPIAIQVATKYSDLIGSQVLIKLFEEYRATEGLYYYLASLVNLTEDKDVVFKFIQAAAKVGQFKEVERIVKTNNVYDPEKVKNFLKDIKLADQMPLVIVCDRFNYIHDLILYLYKAQDFKFIEVYVQQVNPSKTPQVVAALLDVDCDEKVIQNLLGSVLGQVPIGELTAEVEKRNRLKLLLPFLEDTLRQGTQEQAVYNTLAKIYIDSNNSPERFLKDNDQYDTLDVGRYCEKRDPYLAYIAYEKGANDDDLIRITNENSMYKYQARYLLKRSDANLWNKVLDEENIHRRQLIDAVVGTGIGELTDPEPVSLTVQAFMSNGLKGELIKLLEKIILEPSPFNDNPALQGLLMLSAIRYEPSKVSSYIEKLDKFDEDEIAPLCVENGLNEEAFEIYSKNEKHDKALQVLVEDVMSLDRAESYVDKINSPELWSQLGAAQLVGLRIPEAINSYIKANDPSNYETVIEVAEQAEKYEELIPYLSMARSSLKEPKIEGALILSYAKLGKLHEIENLLTGSTVADLESVGNKLFEEKDYKAAKLCFSAVSNYSRLATTLVYLNDYQAAVDTARKASNVRVWREVGDACVDNKEFRLAQICGLNLIVHAEELEELVEKYESHGYFEELISLFEAGLGLERAHMGMFTELAILYTKYDMGKTYEHLKLFWSRINIPKVIRAVESAHLWTELIFLYAHYDEWDNAALTMIDKSADDFDHSYFKEIIVKVSNLEIYYKAINFYVKEHPTLLIDLMTVLSSKLDIPRTIRIFSKSDNLPLIKPFLVNVLPKNNSVVNQAYHDLMIEEEDYKALQVAVDSYDKFDQLDLAARLEKHDLIFFKKIAAQLYRRNKKWSKSLAILREEKLWADAIETAAISQSTEVAEELLNYFVDTNNREGFVALLYTAYHLIRYEVVLELAWMNSLEDLIKPYEISVKKEQYSNVQKLLEQSKSGAKSDAAQSEDQPLLLTNGAMAYQATGQAFGNYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLZ8</accession>
    <name>C5DLZ8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Guanylate kinase</fullName>
            <ecNumber evidence="2">2.7.4.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">GMP kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0G04796g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the guanylate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.8" evidence="2"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555246.1">
        <property type="nucleotide sequence ID" value="XM_002555200.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLZ8"/>
    <dbReference type="FunCoup" id="C5DLZ8">
        <property type="interactions" value="595"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLZ8"/>
    <dbReference type="GeneID" id="8293512"/>
    <dbReference type="KEGG" id="lth:KLTH0G04796g"/>
    <dbReference type="eggNOG" id="KOG0707">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001715_0_1_1"/>
    <dbReference type="InParanoid" id="C5DLZ8"/>
    <dbReference type="OMA" id="EWAVVHG"/>
    <dbReference type="OrthoDB" id="6334211at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004385">
        <property type="term" value="F:GMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00071">
        <property type="entry name" value="GMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.63.10:FF:000002">
        <property type="entry name" value="Guanylate kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000776">
        <property type="entry name" value="Guanylate kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.63.10">
        <property type="entry name" value="Guanylate Kinase phosphate binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017665">
        <property type="entry name" value="Guanylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020590">
        <property type="entry name" value="Guanylate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03263">
        <property type="entry name" value="guanyl_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23117:SF13">
        <property type="entry name" value="GUANYLATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23117">
        <property type="entry name" value="GUANYLATE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00856">
        <property type="entry name" value="GUANYLATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Guanylate kinase-like" evidence="9">
        <location>
            <begin position="2"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR24809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="185" mass="20504" checksum="BB16ACB4C0B918E2" modified="2009-07-28" version="1">MSRPIVISGPSGTGKSTLLKKLFNEFPNKFGFSVSSTTRSPRPGEVNGTHYNFVTVEQFKEMIADGKFIEWAQFSGNYYGTTIASVNEVTNSGKRCILDIDMQGVKAVKKTDLNARFLFIAPPSVDSLRERLTGRGTETPESLQKRLDAATAEMDYAQTGAHDKIIVNDEVDVAYAQMKEFIFSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>C5DM20</accession>
    <name>C5DM20_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0G05280p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0G05280g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dpy-30 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555268.1">
        <property type="nucleotide sequence ID" value="XM_002555222.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DM20"/>
    <dbReference type="STRING" id="559295.C5DM20"/>
    <dbReference type="GeneID" id="8293534"/>
    <dbReference type="KEGG" id="lth:KLTH0G05280g"/>
    <dbReference type="eggNOG" id="KOG4109">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2441248_0_0_1"/>
    <dbReference type="InParanoid" id="C5DM20"/>
    <dbReference type="OMA" id="QPFNADR"/>
    <dbReference type="OrthoDB" id="417678at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd22965">
        <property type="entry name" value="DD_DPY30_SDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.890.10">
        <property type="entry name" value="cAMP-dependent protein kinase regulatory subunit, dimerization-anchoring domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007858">
        <property type="entry name" value="Dpy-30_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049629">
        <property type="entry name" value="DPY30_SDC1_DD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05186">
        <property type="entry name" value="Dpy-30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="67"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="75"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR24831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="90" mass="9910" checksum="AFC45F286B82C625" modified="2009-07-28" version="1">MDPEQAPKQESPQPFNADRVDFAETIGGSHTRKYLNQHVTPALLEGMRLVAKNKPSDPLRYLGEYLIKAGEDQDQPTNSSSQPNGSASQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DM52</accession>
    <name>C5DM52_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein BFR2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0G06050g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AATF family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555300.1">
        <property type="nucleotide sequence ID" value="XM_002555254.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DM52"/>
    <dbReference type="FunCoup" id="C5DM52">
        <property type="interactions" value="1019"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DM52"/>
    <dbReference type="GeneID" id="8293568"/>
    <dbReference type="KEGG" id="lth:KLTH0G06050g"/>
    <dbReference type="eggNOG" id="KOG2773">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018299_1_1_1"/>
    <dbReference type="InParanoid" id="C5DM52"/>
    <dbReference type="OMA" id="INFMAPN"/>
    <dbReference type="OrthoDB" id="5783963at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025160">
        <property type="entry name" value="AATF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039223">
        <property type="entry name" value="AATF/Bfr2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012617">
        <property type="entry name" value="AATF_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15565">
        <property type="entry name" value="AATF PROTEIN APOPTOSIS ANTAGONIZING TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15565:SF0">
        <property type="entry name" value="PROTEIN AATF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13339">
        <property type="entry name" value="AATF-Che1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08164">
        <property type="entry name" value="TRAUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AATF leucine zipper-containing" evidence="5">
        <location>
            <begin position="185"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="Apoptosis-antagonizing transcription factor C-terminal" evidence="4">
        <location>
            <begin position="414"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="354"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="28"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="59"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="137"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="384"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR24863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="531" mass="57829" checksum="41E3AF0EEEF4912E" modified="2009-07-28" version="1">MAKSISDQIAAIANEPAVPDYDIEDEDVFQHREHSGDELSESEDDTQAKSHYVSVGKSRLRDQGIALKDEKYSGQKGSRKALSEGQSEGESEVDSESEIESESESEIETENKRRGSEPADESDGSSNSESDGKSDDGGDSESSEDSADEGVSADQGDERRRRLAALVQRDARDAVARLSDTTRRDAAKGLAVLQQTREFDRILDARIKLQKAVAAANRLPVSAQAWDGHLRESETNAALLSETMRLLEKVLAQTLDFRAELQDAAPTGSDAAATRKRTFSDLCEDSRGLDAKLREFRVAVLSKWSQRVAAASGQSVLAASKFKAIHQPAHVQVETQLADMPRLLRRTALNRRDVAPLGQSEHNIAGGGDVSNDTPAISDGPEPDIPRDFDANARRGRTADTTTDSYVFDDEDFYRVLLNDLVDKKVAGASGDSAVAITSRATANKKLHKNVDTKASKGRKLNFTVQEPIANYEAPANGGLRWSDEQIDEFCAGLLGQRVSFAEDEKEEDRDATEETADGELLQADGIRLFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DM70</accession>
    <name>C5DM70_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mediator of RNA polymerase II transcription subunit 20</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Mediator complex subunit 20</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">MED20</name>
        <name evidence="6" type="ordered locus">KLTH0G06446g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the Mediator complex subunit 20 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DM70"/>
    <dbReference type="FunCoup" id="C5DM70">
        <property type="interactions" value="278"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DM70"/>
    <dbReference type="KEGG" id="lth:KLTH0G06446g"/>
    <dbReference type="eggNOG" id="ENOG502RXMU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065844_1_0_1"/>
    <dbReference type="InParanoid" id="C5DM70"/>
    <dbReference type="OMA" id="WTQRQSI"/>
    <dbReference type="OrthoDB" id="1854899at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.180">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013921">
        <property type="entry name" value="Mediator_Med20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000082">
        <property type="entry name" value="SEA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08612">
        <property type="entry name" value="Med20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50024">
        <property type="entry name" value="SEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="SEA" evidence="5">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR24881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="212" mass="23775" checksum="ECAB7D13B02242CB" modified="2009-07-28" version="1">MHSTVIFSEKATPASITEFHDILSNDLVSIKEKWSFEFKTYRLTVKNLPQGSSKLMHVITFTNRDNQSVIIKNNSAIVTSPATEGVQPSLTLNGCSAGTLDTFDNLLASKLSNLWTQRQNIKGDFGSTFKTTELTVRATNVFSYGGFKGLLIELECEDDVSDGEFEQRVMKIRHLLDDMLTGEYRVCSASMDPKNRNLICDLAFQYVKVLEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>C5DM77</accession>
    <name>C5DM77_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0G06622p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0G06622g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RMD1/sif2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555325.1">
        <property type="nucleotide sequence ID" value="XM_002555279.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DM77"/>
    <dbReference type="FunCoup" id="C5DM77">
        <property type="interactions" value="89"/>
    </dbReference>
    <dbReference type="GeneID" id="8293594"/>
    <dbReference type="KEGG" id="lth:KLTH0G06622g"/>
    <dbReference type="eggNOG" id="KOG2861">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011220_0_0_1"/>
    <dbReference type="InParanoid" id="C5DM77"/>
    <dbReference type="OMA" id="QGLYQTK"/>
    <dbReference type="OrthoDB" id="242766at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070131">
        <property type="term" value="P:positive regulation of mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003734">
        <property type="entry name" value="DUF155"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051624">
        <property type="entry name" value="RMD1/Sad1-interacting"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16255">
        <property type="entry name" value="REQUIRED FOR MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16255:SF1">
        <property type="entry name" value="REQUIRED FOR MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02582">
        <property type="entry name" value="DUF155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF155" evidence="2">
        <location>
            <begin position="141"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR24888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="392" mass="44640" checksum="FEB3FC47171C8E34" modified="2009-07-28" version="1">MLRCFTFSRTFPTNVQLLVPKRILSFTVKILQANNEIGTSKVRSVKVPRGSLSAAKNLRRSFLPNKKGTHSQMVVNNPNTNKLAYFLKTKPCTTVTTSESYDLNKVISSLNASGYHAISLVPDEIITFKYFYNGCHSDIMVLGNSGTVVAWGLEESFVFENILPLIEHGRVNPLTEECVESEDLDFLQVENAEDLEKVRQITNRSAPESFVEGDLIIVNAIDSDLGLLDKAAFSSGMSRSTRLAVLEHLLEAHIEKSRKFTELLSQGKKLSLTESEVLQSTGSLFLIRGKLNLYSELIETPDLYWSEPQLERIYKQMSRSLDIQPRISILNTKLDYATEESRAFMAVLNEKKSTHLEWIIIYLITVEVFFELHHYYERYWVDSKEDCVRTTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMB7</accession>
    <name>C5DMB7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">26S proteasome regulatory subunit 7 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0G07524g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555365.1">
        <property type="nucleotide sequence ID" value="XM_002555319.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMB7"/>
    <dbReference type="FunCoup" id="C5DMB7">
        <property type="interactions" value="1179"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMB7"/>
    <dbReference type="GeneID" id="8293635"/>
    <dbReference type="KEGG" id="lth:KLTH0G07524g"/>
    <dbReference type="eggNOG" id="KOG0729">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_6_0_1"/>
    <dbReference type="InParanoid" id="C5DMB7"/>
    <dbReference type="OMA" id="RSKYHIE"/>
    <dbReference type="OrthoDB" id="1937997at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010604">
        <property type="term" value="P:positive regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070682">
        <property type="term" value="P:proteasome regulatory particle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080090">
        <property type="term" value="P:regulation of primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19502">
        <property type="entry name" value="RecA-like_PAN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000005">
        <property type="entry name" value="26S protease regulatory subunit 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000027">
        <property type="entry name" value="26S protease regulatory subunit 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000329">
        <property type="entry name" value="26S protease subunit component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048723">
        <property type="entry name" value="OB_PRS7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21236">
        <property type="entry name" value="OB_PRS7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0647">Proteasome</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="10">
        <location>
            <begin position="220"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00067449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR24928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="445" mass="49903" checksum="DE8B1446B5A92DD9" modified="2009-07-28" version="1">MPPKEDWEKFKAPAEEEKQEEKPNVPLTEGDIQVLKTYGAAPYADKLKETEKDLKDIEARIKEKAGVKESDTGLAPSHLWDIMGDRQRLSEEHPLQVARCTKIIKPEASDESPADEDEEDSKYIINLKQIAKFVVGLGERVSPTDIEEGMRVGVDRSKYHIELPLPPRIDPSVTMMTVEEKPDVTYSDVGGCKEQIDKLREVVELPLLSPERFAALGIDPPKGILLYGPPGTGKTLCARAVANRTDATFIRVIGSELVQKYVGEGARMVRELFEMARTRKACIIFFDEIDAVGGARFDDGAGGDNEVQRTMLELITQLDGFDPRGNIKVMFATNRPNTLDPALLRPGRIDRKVEFSLPDLEGRANIFRIHSKSMSVERGIRWELISRLCPNSTGAELRSVCTEAGMFAIRARRKVATEKDFLQAVEKVINGYKKFSSTSRYMQYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMF8</accession>
    <name>C5DMF8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0G08536p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0G08536g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the acyl coenzyme A hydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555406.1">
        <property type="nucleotide sequence ID" value="XM_002555360.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMF8"/>
    <dbReference type="STRING" id="559295.C5DMF8"/>
    <dbReference type="GeneID" id="8293680"/>
    <dbReference type="KEGG" id="lth:KLTH0G08536g"/>
    <dbReference type="eggNOG" id="KOG2763">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032862_1_0_1"/>
    <dbReference type="InParanoid" id="C5DMF8"/>
    <dbReference type="OMA" id="RAESHNA"/>
    <dbReference type="OrthoDB" id="331699at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047617">
        <property type="term" value="F:fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006637">
        <property type="term" value="P:acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03442">
        <property type="entry name" value="BFIT_BACH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.10:FF:000032">
        <property type="entry name" value="Acyl-CoA thioester hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033120">
        <property type="entry name" value="HOTDOG_ACOT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12655">
        <property type="entry name" value="ACYL-COA THIOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12655:SF0">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE 9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51770">
        <property type="entry name" value="HOTDOG_ACOT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="HotDog ACOT-type" evidence="6">
        <location>
            <begin position="115"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="HotDog ACOT-type" evidence="6">
        <location>
            <begin position="318"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="20"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="37"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR24969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="524" mass="58511" checksum="5674DB237F26E1DF" modified="2009-07-28" version="1">MLRSLRPRSGTALKTLKPSMPQQRVRMLHHKDDGKSTDASTQPDAAATTLTDKYEHKRLSNVPKTAKGYEATWLNALAERQKQLAQGKIIDSYVYSSVKSTEVGEKTRKDSFAYLALPFREDASVADFYVNAAGRLRMGQIFQDLDALAGRIAYRHTSPAQPVIVTASVDRIYLLKSIDFMADYNIVLSGSVVWTGRSSMEIAIKATAIKGDLPAEITEDSLAAEDTFLTASFTFVARNPETHKSLAVNKLLPLNEKEWMDFRRAESHNAAKKLRASNESLDTNPPTGEESKIIHNMWVASKELSKLSHKPPNVMSMKDTNAKSTMFMQPQYRNRHSYMIFGGYLMRQTFELAYCAAASFSHAFPRFVSLDSTHFKAPVPVGSILHMDATVVHTEHLFDRSSKPKVTTVTHKSADGNTVFHFDSSPVNELSTQKGALLSKPGTLIQVKVDTIVQELASEKKNSSGSFIYSFYVHRDADEKTIGKPGYTTVIPETYSEMIEYISARRRAIDTAAYANTLRRNNKL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-05-03" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMP2</accession>
    <name>UTP25_LACTC</name>
    <protein>
        <recommendedName>
            <fullName>U3 small nucleolar RNA-associated protein 25</fullName>
            <shortName>U3 snoRNA-associated protein 25</shortName>
        </recommendedName>
        <alternativeName>
            <fullName>U three protein 25</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">UTP25</name>
        <name type="ordered locus">KLTH0G10494g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DEAD-box RNA helicase-like protein required for pre-18S rRNA processing, specifically at sites A0, A1, and A2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UTP25 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555490.1">
        <property type="nucleotide sequence ID" value="XM_002555444.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMP2"/>
    <dbReference type="FunCoup" id="C5DMP2">
        <property type="interactions" value="1333"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMP2"/>
    <dbReference type="GeneID" id="8293768"/>
    <dbReference type="KEGG" id="lth:KLTH0G10494g"/>
    <dbReference type="eggNOG" id="KOG2340">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018705_0_1_1"/>
    <dbReference type="InParanoid" id="C5DMP2"/>
    <dbReference type="OMA" id="PSIFGYH"/>
    <dbReference type="OrthoDB" id="10264378at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010678">
        <property type="entry name" value="UTP25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053939">
        <property type="entry name" value="UTP25_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053940">
        <property type="entry name" value="UTP25_NTPase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12933">
        <property type="entry name" value="ORF PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12933:SF0">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 25 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06862">
        <property type="entry name" value="Utp25_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22916">
        <property type="entry name" value="UTP25_NTPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0539">Nucleus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0690">Ribosome biogenesis</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <feature type="chain" id="PRO_0000408119" description="U3 small nucleolar RNA-associated protein 25">
        <location>
            <begin position="1"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="21"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="115"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="747" mass="85710" checksum="F3D8813D230B757F" modified="2009-07-28" version="1">MSSLKRSSSAIKGSNEYRSSKSGRKQLRTIRKASGPRRGLAEESANEENNGSETEAEDKLAAPELNEPAQDQRAGGEAYAALLTLLKAEHGGPARRKTKTDDKKTSADPTSNVKDEEESESRDNEDEEEDEDEEAAIENALMDEHTSGDEDDEGDDGSHQDDINGVTREGGDATELAAGFEEEDKPDTLEIHFNSVSEKDTNALDAAFKAKEVRYRSCKLHVDKNQEFIYSRPTLVNEPASAVSAPDGSQSLNSYFIKQRLKIQNDLLDSQDKLTPLQKQIVDPMFQYQDLLYEYEDYDKETEYRDLYALHVLNHIYKTRDRILKNNQRLQENPDQELLDQGFTRPKVLIVAPTRDAAYDILSKIIQKSGLDQVDKKAKFKDQFFQEALPPSYKPKSFQQLFKGNTNDFFVLGAKFTRKTIKLYSNFYQSDIIICSPLGIQLILENTDKKKRQDDFLSSIELLVVDQLHSIEFQNMLHLTSIFEHINKIPQQQHDADFSRIKMWYINDQAKLFRQTLIFTKFSSPFANSLINGKCRNYAGRWKNHRVIFPENSSLGQLGMRTRLIFQRFDLVGKSVSEEPDSRFKHFCSVIVPNIVKSTGYEDGILLYIPDYTDFVRVRNYLREKTTILFGDINEYSEQRQLTSNRAMFQQGRVKVLLYTERLHHFRRYEIKGVKTVIFYKPPTNPEFFEEVARYLGKSAFLGLADLNISVVRCLFSKLDGLSLERIVGTERAAVLTHGPNETYEFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMR3</accession>
    <name>C5DMR3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Alpha-mannosidase</fullName>
            <ecNumber evidence="3">3.2.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0G11000g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Degrades free oligosaccharides in the vacuole.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.</text>
            <dbReference type="EC" id="3.2.1.24"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 38 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.24" evidence="3"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555511.1">
        <property type="nucleotide sequence ID" value="XM_002555465.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMR3"/>
    <dbReference type="FunCoup" id="C5DMR3">
        <property type="interactions" value="292"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMR3"/>
    <dbReference type="GeneID" id="8293790"/>
    <dbReference type="KEGG" id="lth:KLTH0G11000g"/>
    <dbReference type="eggNOG" id="KOG4342">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003442_0_1_1"/>
    <dbReference type="InParanoid" id="C5DMR3"/>
    <dbReference type="OMA" id="GQYWDAW"/>
    <dbReference type="OrthoDB" id="10261055at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004559">
        <property type="term" value="F:alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006013">
        <property type="term" value="P:mannose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009313">
        <property type="term" value="P:oligosaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.50:FF:000004">
        <property type="entry name" value="alpha-mannosidase 2C1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.110.10:FF:000002">
        <property type="entry name" value="alpha-mannosidase 2C1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.98.30:FF:000001">
        <property type="entry name" value="alpha-mannosidase 2C1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.110.10">
        <property type="entry name" value="Glycoside hydrolase 38, N terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.50">
        <property type="entry name" value="Glycoside hydrolase family 38, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.30">
        <property type="entry name" value="Golgi alpha-mannosidase II, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054723">
        <property type="entry name" value="Ams1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041147">
        <property type="entry name" value="GH38_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011682">
        <property type="entry name" value="Glyco_hydro_38_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015341">
        <property type="entry name" value="Glyco_hydro_38_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037094">
        <property type="entry name" value="Glyco_hydro_38_cen_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000602">
        <property type="entry name" value="Glyco_hydro_38_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027291">
        <property type="entry name" value="Glyco_hydro_38_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028995">
        <property type="entry name" value="Glyco_hydro_57/38_cen_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46017">
        <property type="entry name" value="ALPHA-MANNOSIDASE 2C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46017:SF1">
        <property type="entry name" value="ALPHA-MANNOSIDASE 2C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09261">
        <property type="entry name" value="Alpha-mann_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22907">
        <property type="entry name" value="Ams1-like_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17677">
        <property type="entry name" value="Glyco_hydro38C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07748">
        <property type="entry name" value="Glyco_hydro_38C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01074">
        <property type="entry name" value="Glyco_hydro_38N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00872">
        <property type="entry name" value="Alpha-mann_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88688">
        <property type="entry name" value="Families 57/38 glycoside transferase middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 38 central" evidence="9">
        <location>
            <begin position="565"/>
            <end position="645"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00054985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00071615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR25074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1082" mass="123402" checksum="D5005C6E162B08E0" modified="2009-07-28" version="1">MSYAQLNSDPQFKPVQGIYEGRLRQFTDGGGEYKELNLPKFYDKKRISLDQDSVKVQWYQVRFEKGSSPVSPDKRPPWSEIVEKDKNKELEFRDAQKGQPFGPSWSTTWFRVQVTVPDEWLAAEEQLIFEWDCSNEGIVIDPETLIPKTAFSGGGERNEYMLPKGQKHHVFYIESGNNGMFGCGAGSSINPPDNNRYFHLNKADLVWPNWEARALFIDFWMLSDAARELPGDSWQKHKARHVGNSVMDLFDPEDASTVTKCRKLIREEYFDQLADDPKVFQAGDSSVRADVHGVGNCHIDTAWLWPFAETRRKVVRSWTSQCTLMDQYPEYRFVASQAQQFKWLLKEHSNFFHEVLIPKIQQSQFFPIGGSWVENDTNIPSGESLCRQFFLGQRFYLKHFGKKSDVFWLPDTFGYSSQVPQIAKISGIDKFLTQKLSWNNINSFPHTTFNWAGIDGSQLLTHMPPGNTYTASSHFGDVLRTAKGNKNSDVYGSGLMLYGKGDGGGGPTAEMLEKMRRIRSISNRNGNVIPKLQVGTTIEEFYQDILEKTHNGLDLPTWVGELYFEFHRGTYTTQAEVKRLMRLSEIKMHDLEWIATKASLVYPDEYEYPINKINRIWEDILLCQFHDVLPGSCIELVYKYEARPMLKDAITEIESLINDALKFLKKKGKGNSSIGTLELVESDATNSAKNEREFVKLKDEAKHIKMTNDVLEVTIDKEKGVISSIKDREREVEYLDLDGGRNKRGANQFVLFDDKPLGWQAWDTELYSVNQYKYITSLESVEIAENSSEKCAVELTFKVSNDCEILTSISLGSRTQHESGGLIEIKTSVKNWNLRNKFLKVEFPVNIRNDFASYETQFGITKRPTHYNTSWDVAKFEVCHHKFADYSEFSKGVSVINDSKYGFATHGNLMRLSLLRSPKAPDAHADMGSHEIRYALYPHRGALSSSTVHEALKFNYRHHYSIPESVSEAFDGVIGIEGDPNVILTNIKRGEDDEGLDSEYSTTKGEKRSFVVRLYESLGGESFAVLKTSLPVKNALKVDNLELSEINSIPLTTKSSASSDKSTLEFKVKLRPFEIATYKLVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMT3</accession>
    <name>C5DMT3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0G11440p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0G11440g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555531.1">
        <property type="nucleotide sequence ID" value="XM_002555485.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMT3"/>
    <dbReference type="GeneID" id="8293810"/>
    <dbReference type="KEGG" id="lth:KLTH0G11440g"/>
    <dbReference type="HOGENOM" id="CLU_2347070_0_0_1"/>
    <dbReference type="InParanoid" id="C5DMT3"/>
    <dbReference type="OrthoDB" id="4036582at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057785">
        <property type="entry name" value="YLR146W-A-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23482">
        <property type="entry name" value="YLR146W-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR25094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="97" mass="10504" checksum="813F4298817FC536" modified="2009-07-28" version="1">MSSSNSSLSSPKVRRLSEGESLAGEMTSSDHSLSIADSQISDILKELTGLQSEVVALHSSLKESNEQIRRDIQDFCSIGENDLSPSNATSSFHEFQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5DN24</accession>
    <name>C5DN24_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein bL34m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0G13574g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL34 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555622.1">
        <property type="nucleotide sequence ID" value="XM_002555576.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DN24"/>
    <dbReference type="FunCoup" id="C5DN24">
        <property type="interactions" value="194"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DN24"/>
    <dbReference type="GeneID" id="8293905"/>
    <dbReference type="KEGG" id="lth:KLTH0G13574g"/>
    <dbReference type="eggNOG" id="KOG4612">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_129938_0_1_1"/>
    <dbReference type="InParanoid" id="C5DN24"/>
    <dbReference type="OMA" id="GRWYLAH"/>
    <dbReference type="OrthoDB" id="431691at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.3980:FF:000001">
        <property type="entry name" value="Mitochondrial ribosomal protein L34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3980">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00391">
        <property type="entry name" value="Ribosomal_bL34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000271">
        <property type="entry name" value="Ribosomal_bL34"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01030">
        <property type="entry name" value="rpmH_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14503:SF4">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL34M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14503">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOMAL PROTEIN 34 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00468">
        <property type="entry name" value="Ribosomal_L34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR25185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="112" mass="12916" checksum="C37970721CD59A19" modified="2009-07-28" version="1">MSFIYNRLFQFSARRSLSSLSSFSPLNAVSCRPSVFQAAAPMAAERSSQGSSPLFSALFGFTQRRWKSRGNTYQPSTLKRKRRVGFLARAKSKQGYKVLKRRREKGRWYLTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>C5DN81</accession>
    <name>C5DN81_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0G14872p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0G14872g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555679.1">
        <property type="nucleotide sequence ID" value="XM_002555633.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DN81"/>
    <dbReference type="FunCoup" id="C5DN81">
        <property type="interactions" value="77"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DN81"/>
    <dbReference type="GeneID" id="8293964"/>
    <dbReference type="KEGG" id="lth:KLTH0G14872g"/>
    <dbReference type="eggNOG" id="ENOG502RZEU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111698_0_0_1"/>
    <dbReference type="InParanoid" id="C5DN81"/>
    <dbReference type="OMA" id="KMYTLAI"/>
    <dbReference type="OrthoDB" id="433738at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR25242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="196" mass="22276" checksum="DADD695EFEB51799" modified="2009-07-28" version="1">MSTDLSELQYNPNSKLLGLPHSSEDVTMNISQINKLTQDLVAETNANFTPQPHAESTAMIKKMFENGLKQTQQQKLADALKSINLAIEMAQRKRTTWEAFAVQLQELQFMLKNRVDLCLVQGRFMDALQDLDFLQNTGLNSSDVFIRKTDALIKLKQYEQARAECERGLSLDPTNVKLKALLIENTRRLAEYNGDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNA5</accession>
    <name>C5DNA5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0G15422p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0G15422g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555703.1">
        <property type="nucleotide sequence ID" value="XM_002555657.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNA5"/>
    <dbReference type="FunCoup" id="C5DNA5">
        <property type="interactions" value="537"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNA5"/>
    <dbReference type="GeneID" id="8293989"/>
    <dbReference type="KEGG" id="lth:KLTH0G15422g"/>
    <dbReference type="eggNOG" id="KOG2704">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011340_5_0_1"/>
    <dbReference type="InParanoid" id="C5DNA5"/>
    <dbReference type="OMA" id="WHGTRPG"/>
    <dbReference type="OrthoDB" id="286734at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003841">
        <property type="term" value="F:1-acylglycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047184">
        <property type="term" value="F:1-acylglycerophosphocholine O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046474">
        <property type="term" value="P:glycerophospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030258">
        <property type="term" value="P:lipid modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049941">
        <property type="entry name" value="LPLAT_7/PORCN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13906:SF4">
        <property type="entry name" value="LYSOPHOSPHOLIPID ACYLTRANSFERASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13906">
        <property type="entry name" value="PORCUPINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="51"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="234"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="424"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="460"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="579"/>
            <end position="606"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR25266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="606" mass="70302" checksum="AFBF48E1A87A3706" modified="2009-07-28" version="1">MGHPIEAKILALSQRTGLDEVTLKLAICLFTSFPFNALLKRLPDHRVNLKCWYIISVAAFYLFGVLNIFTGFRTLFVSTMFTYVITRFYRSRFMPYVNFVGVMVHLAVNHLGAQFFGTADPSKVDITGAQMVLVMKLSSFAWSYHDGTNADHAHLTDHQKSRAISHHPSLLSFLAYAFFYPSLFTGPSFDFADFESWLNCEVFRDLPDARKPRRRWTTRDRNLRRQIPKCGGLVLWRIAQGIFWITLSSVLPNYISKEYMFTPEYANKSFFYKIHYLYILGFTFRLKYYAAWTMSEASCILCGLGYNGYDPKTKKIRWDRVRNIDIWKFETASNTHEGLEAWNMNTNKWLKYYVYLRVTKPGAKPGFRSTLFTFLTSAFWHGTRPGYYLSFATGALYQTCGKIYRRNLRPLFLAADGSALPTKIIYDVICFYLMKLAFGYLVQPFVILELPGSLACWRSVYFYIHLGIAATFFIFKGPFAKPVLKFIKSHHPAVVAEEKQKQQLEEAGPLGDIVKAKNLYEQEESSSNMHLGIPDADFDRFNLDEAKKEFKEFLESYEKWRSEKGLEIEEQNLQKAFENFKEEMGPGKTRRTSFSSYSPKPIQKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNK2</accession>
    <name>C5DNK2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">1,3-beta-glucanosyltransferase</fullName>
            <ecNumber evidence="8">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0G17732g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Splits internally a 1,3-beta-glucan molecule and transfers the newly generated reducing end (the donor) to the non-reducing end of another 1,3-beta-glucan molecule (the acceptor) forming a 1,3-beta linkage, resulting in the elongation of 1,3-beta-glucan chains in the cell wall.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Lipid-anchor</topology>
            <topology evidence="8">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the glycosyl hydrolase 72 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="8"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555800.1">
        <property type="nucleotide sequence ID" value="XM_002555754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNK2"/>
    <dbReference type="FunCoup" id="C5DNK2">
        <property type="interactions" value="64"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNK2"/>
    <dbReference type="GeneID" id="8294094"/>
    <dbReference type="KEGG" id="lth:KLTH0G17732g"/>
    <dbReference type="eggNOG" id="ENOG502QRZZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021855_2_0_1"/>
    <dbReference type="InParanoid" id="C5DNK2"/>
    <dbReference type="OMA" id="ICLRDIP"/>
    <dbReference type="OrthoDB" id="421038at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042124">
        <property type="term" value="F:1,3-beta-glucanosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071970">
        <property type="term" value="P:fungal-type cell wall (1-&gt;3)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000038">
        <property type="entry name" value="1,3-beta-glucanosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004886">
        <property type="entry name" value="Glucanosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012946">
        <property type="entry name" value="X8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31468">
        <property type="entry name" value="1,3-BETA-GLUCANOSYLTRANSFERASE GAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31468:SF10">
        <property type="entry name" value="1,3-BETA-GLUCANOSYLTRANSFERASE GAS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03198">
        <property type="entry name" value="Glyco_hydro_72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07983">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00768">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 8" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005125175" description="1,3-beta-glucanosyltransferase" evidence="8">
        <location>
            <begin position="21"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="domain" description="X8" evidence="10">
        <location>
            <begin position="404"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="352"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="352"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR25363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="548" mass="62084" checksum="05D48616F2721522" modified="2009-07-28" version="1">MNIMLLLLNIFITYFMFASGVIKTEDFKLEYPTSISDLPAIEVHGDKFFNSMTNEQFFMKGIAYQPSYSMNDLKLLEGGAMDTKYIDPLASPDLCLRDIPHLVELGVNTIRVYAIDPTKSHDVCMKELAANGIYVLLDLSEPDNSISRDSPTWDVSVYQRYKDVVDSMHQYSNVLGFFAGNEVTNDITNTDASPFVKASIRDIKGHISENGYRSIPIGYSTNDDIETRENLAQFFICGDSVADFYGINMYEWCGYSSYHSSGYDQRTKEFKDYPVPIFFSEFGCNSVRPRPFTEVEALYGPHMTKVWSGGPAYMYFEEENNYGVVKIDEDNEVVHLKDFDFLKDEFSRAKPKGITREEHTRKQGSDPRRKKRRECPAISKTWKAAATIPDTPSQTKCSCLEESLPCLVQPFHDPSRYKDYFEYVCSQVDCSDIKADGEKGQYGEFSDCSTNQKLALEISKMYYLDPKSSGTCPIVDGQIHYNTKSKQAPQDKRCAAIIKTVRSASKATNGDTRQLHQNSNERASSGSRNAAKWFSLTLALGILMFSFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNP8</accession>
    <name>C5DNP8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">KLTH0G18854p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0G18854g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555846.1">
        <property type="nucleotide sequence ID" value="XM_002555800.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNP8"/>
    <dbReference type="FunCoup" id="C5DNP8">
        <property type="interactions" value="84"/>
    </dbReference>
    <dbReference type="GeneID" id="8294141"/>
    <dbReference type="KEGG" id="lth:KLTH0G18854g"/>
    <dbReference type="eggNOG" id="KOG0254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_30_12_1"/>
    <dbReference type="InParanoid" id="C5DNP8"/>
    <dbReference type="OMA" id="ITYICEM"/>
    <dbReference type="OrthoDB" id="4142200at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF7">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="110"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="143"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="170"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="260"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="296"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="324"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="367"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="407"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="435"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="12"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="482"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="482"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="493"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR25409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="502" mass="54731" checksum="9BA5D482CF53BA69" modified="2009-07-28" version="1">MKRNPDYGFGKIIGTVAVAGTILGMDVSSMTVFLTSSYFNQYFNFPGPALQGLITGANPVGGLVGCILFGIMTDKLGRAPTFRVLALIWIIGSIISAAVLNVWMLIAGRFIRGIAVGSFSVLLPVYIGEVVPEQRRGLATSVVQLALTSAILVVFFVCFLLNYFEHQYSFRIAWGLEMVPALLLLVLSFSLLESPKWLASHGRYDQARTILQNSGVIENHDREEVDAAPVTKIDILELYGGSQKISYGDLFSGTLRKHMAVGVTVQVLVQACGINILMFYIVYICEMIGLRGGTKLAAASVPYFINVVFTCIPIALLDRLERKAVVSFGGLSLGFIMALMGAIMGWFGHSVLPVNGNATIVWEITGAPGFLILALCFLFVALFASTLSCCAWLYSSEVLPRDARSKGMALCMAASWFLNSCLTFTTPLLLRKIKWITFVLLGSTTVLLSIVVAVWFPETKGLSEDQIKQIFRKPAIEIELGEKEKYSDESDTRSSAPSPVTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5E215</accession>
    <name>C5E215_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0H01386p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0H01386g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555938.1">
        <property type="nucleotide sequence ID" value="XM_002555892.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E215"/>
    <dbReference type="FunCoup" id="C5E215">
        <property type="interactions" value="86"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E215"/>
    <dbReference type="GeneID" id="8294251"/>
    <dbReference type="KEGG" id="lth:KLTH0H01386g"/>
    <dbReference type="eggNOG" id="KOG4480">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049906_0_0_1"/>
    <dbReference type="InParanoid" id="C5E215"/>
    <dbReference type="OMA" id="FAFAFQM"/>
    <dbReference type="OrthoDB" id="652091at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004392">
        <property type="term" value="F:heme oxygenase (decyclizing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006788">
        <property type="term" value="P:heme oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19165">
        <property type="entry name" value="HemeO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.910.10">
        <property type="entry name" value="Heme oxygenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002051">
        <property type="entry name" value="Haem_Oase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016053">
        <property type="entry name" value="Haem_Oase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016084">
        <property type="entry name" value="Haem_Oase-like_multi-hlx"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10720">
        <property type="entry name" value="HEME OXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10720:SF0">
        <property type="entry name" value="HEME OXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01126">
        <property type="entry name" value="Heme_oxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000343">
        <property type="entry name" value="Haem_Oase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48613">
        <property type="entry name" value="Heme oxygenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0349">Heme</keyword>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="146"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="286"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="4">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000343-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR30076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="307" mass="35870" checksum="E88C5CFA4CD07959" modified="2009-07-28" version="1">MTTIPSPTDVGALANRINFHTRDQHNKIDKQMSAKFAFAMKHGFIYRQGVLAFFYVFQAIEQELDRLRQEAQTPEEEQTRDILNQFWTEEFRRAPRILQDLELLYAKEFPTSASLRTFLEETTLAPELQGFVDYVHASIRSRPTAILAYCHVLYLALFAGGRVMRSTIYRSTGLFPKFPNLTTEEVVRRGTNFFTFSDAGVQAENQLRKNYKRGYELATRQGLTEEQKLNILDVSNAIFEWNTRVVTEIGEINRQELMGKLSFRLLTFVIEEWQYSDKLTRKQKSLVLATGVVLQILMMYWVLRKFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>C5E216</accession>
    <name>C5E216_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">DNA ligase</fullName>
            <ecNumber evidence="14">6.5.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">KLTH0H01408g</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + diphosphate.</text>
            <dbReference type="EC" id="6.5.1.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the ATP-dependent DNA ligase family.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.1" evidence="14"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555939.1">
        <property type="nucleotide sequence ID" value="XM_002555893.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E216"/>
    <dbReference type="FunCoup" id="C5E216">
        <property type="interactions" value="745"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E216"/>
    <dbReference type="GeneID" id="8294252"/>
    <dbReference type="KEGG" id="lth:KLTH0H01408g"/>
    <dbReference type="eggNOG" id="KOG0967">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005138_4_1_1"/>
    <dbReference type="InParanoid" id="C5E216"/>
    <dbReference type="OMA" id="WIKYKRD"/>
    <dbReference type="OrthoDB" id="206088at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003910">
        <property type="term" value="F:DNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903461">
        <property type="term" value="P:Okazaki fragment processing involved in mitotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07900">
        <property type="entry name" value="Adenylation_DNA_ligase_I_Euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07969">
        <property type="entry name" value="OBF_DNA_ligase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3260.10:FF:000001">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000062">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.30:FF:000002">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3260.10">
        <property type="entry name" value="DNA ligase, ATP-dependent, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.30">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050191">
        <property type="entry name" value="ATP-dep_DNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000977">
        <property type="entry name" value="DNA_ligase_ATP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012309">
        <property type="entry name" value="DNA_ligase_ATP-dep_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012310">
        <property type="entry name" value="DNA_ligase_ATP-dep_cent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016059">
        <property type="entry name" value="DNA_ligase_ATP-dep_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012308">
        <property type="entry name" value="DNA_ligase_ATP-dep_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036599">
        <property type="entry name" value="DNA_ligase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00574">
        <property type="entry name" value="dnl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674:SF4">
        <property type="entry name" value="DNA LIGASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674">
        <property type="entry name" value="DNA LIGASE 1/3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04679">
        <property type="entry name" value="DNA_ligase_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01068">
        <property type="entry name" value="DNA_ligase_A_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04675">
        <property type="entry name" value="DNA_ligase_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117018">
        <property type="entry name" value="ATP-dependent DNA ligase DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56091">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00697">
        <property type="entry name" value="DNA_LIGASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00333">
        <property type="entry name" value="DNA_LIGASE_A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50160">
        <property type="entry name" value="DNA_LIGASE_A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="7 14" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9 14" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="10 14" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3 14" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-dependent DNA ligase family profile" evidence="17">
        <location>
            <begin position="468"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="16"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="702"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="27"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="71"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="705"/>
            <end position="723"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAR30077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="723" mass="80073" checksum="EF9EDC317480A65A" modified="2009-07-28" version="1">MLRLVRAEMSSIKKQATLAKFFSSKKRSVEEEKSHEPAKKLHSDPIGAGSGSPSSSPPLATGSSDAGSVKVETSATSTGENTKAGTPTLPDSDEPAKRAVAATKGDPAKLGASAPKDSKILYAEMCNVFNEIEGTSSRLQIVKLCGDFLYSVLLKARKDIVPITYLLINKLGPDYQPGLELGLGEGLLMKTISESCGKSLQQIKARYRELGDLGRIAQEARAVQPTMFKPKPLTTGEVFSNLQAIARSQGKDSQLRKVQLIKRMLTACQGLEAKFLIRSLESKLRIGLAEKSVLVALSKALLTYEHNGKEPSSELVDTAEQKIRDAFCQVPNYQIVIETALEHGIMELDKHCVLTPGIPLKPMLAKPSKSISEVLDRFQGQRFTCEYKYDGERAQVHLLPDGSMRIYSRNGENMTERYPEIRISDFIADPARTHTLILDCEAVAWDKEKNTILPFQVLSTRKRKGVAVEDVKVRVCLFAFDILCYNDEPLITRPLAERRACLERALVPVPGELQLASEVTTMSLDELQHYLDQAVRDCCEGLMVKILEGEESHYEPSKRSRNWLKLKKDYLQGVGDSLDLCVLGAYYGRGKRTGTYGGFLLGCYNQDSGEYETCCKIGTGFSEEMLQTLFDRLSPTAIPTPKAFYVHSESAPPDVWFEPTLLFEVLTADLSLSPVYKAGTSSYGKGISLRFPRFIRLRDDKTPEEATSSEQVVEFYESQSHVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5E271</accession>
    <name>C5E271_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein bL28m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0H02618g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL28 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555994.1">
        <property type="nucleotide sequence ID" value="XM_002555948.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E271"/>
    <dbReference type="FunCoup" id="C5E271">
        <property type="interactions" value="270"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E271"/>
    <dbReference type="GeneID" id="8294307"/>
    <dbReference type="KEGG" id="lth:KLTH0H02618g"/>
    <dbReference type="eggNOG" id="KOG3278">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090033_0_0_1"/>
    <dbReference type="InParanoid" id="C5E271"/>
    <dbReference type="OMA" id="IKMTAKV"/>
    <dbReference type="OrthoDB" id="361870at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.170.40:FF:000003">
        <property type="entry name" value="54S ribosomal protein L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.170.40">
        <property type="entry name" value="Ribosomal protein L28/L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00373">
        <property type="entry name" value="Ribosomal_bL28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026569">
        <property type="entry name" value="Ribosomal_bL28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034704">
        <property type="entry name" value="Ribosomal_bL28/bL31-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037147">
        <property type="entry name" value="Ribosomal_bL28_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13528">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L28, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13528:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL28M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00830">
        <property type="entry name" value="Ribosomal_L28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143800">
        <property type="entry name" value="L28p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR30132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="273" mass="31282" checksum="53235733108692F8" modified="2009-07-28" version="1">MLEHRHGDPRASDTTQAMSVNWPRLQRGFSTIPCLFREWRLVECRNVAKKPDYKVGDARPIFVPKARKAFPDYEFGESQIFKQSNKGLYGGSFIQYGNNVAESKTKTRRRWLPNVIKKGLWSEALSRNVSIRMTAKVLRTVTKEGGIDNYLTKEKSARIKELGPTGWKLRYRVLQRQELRENPPHKDAAVVRGIDGAEHTVYFEEALDGETVRVTCGRRKLLQLLYPLEKLEHRADGETLTYKQFLDAYAGTSVRDILAALSSYGFDLKTITV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>C5E293</accession>
    <name>C5E293_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">RuvB-like helicase</fullName>
            <ecNumber evidence="15">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">KLTH0H03102g</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">DNA helicase participates in several chromatin remodeling complexes, including the SWR1 and the INO80 complexes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the RuvB family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="15"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556016.1">
        <property type="nucleotide sequence ID" value="XM_002555970.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E293"/>
    <dbReference type="FunCoup" id="C5E293">
        <property type="interactions" value="1740"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E293"/>
    <dbReference type="GeneID" id="8294329"/>
    <dbReference type="KEGG" id="lth:KLTH0H03102g"/>
    <dbReference type="eggNOG" id="KOG2680">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028311_4_0_1"/>
    <dbReference type="InParanoid" id="C5E293"/>
    <dbReference type="OMA" id="IINTEPY"/>
    <dbReference type="OrthoDB" id="10060499at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097255">
        <property type="term" value="C:R2TP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000812">
        <property type="term" value="C:Swr1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000492">
        <property type="term" value="P:box C/D snoRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002221">
        <property type="entry name" value="RuvB-like 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000010">
        <property type="entry name" value="RuvB-like helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.360:FF:000002">
        <property type="entry name" value="RuvB-like helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.360">
        <property type="entry name" value="RuvB-like helicase, domain II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027238">
        <property type="entry name" value="RuvB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041048">
        <property type="entry name" value="RuvB-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042487">
        <property type="entry name" value="RuvBL1/2_DNA/RNA_bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010339">
        <property type="entry name" value="TIP49_P-loop"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11093">
        <property type="entry name" value="RUVB-RELATED REPTIN AND PONTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06068">
        <property type="entry name" value="TIP49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17856">
        <property type="entry name" value="TIP49_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0010">Activator</keyword>
    <keyword evidence="7 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 15" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4 15" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12 15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6 15" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 15" id="KW-0804">Transcription</keyword>
    <keyword evidence="9 15" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="16">
        <location>
            <begin position="67"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU363048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAR30154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="470" mass="51275" checksum="D94E4618A4AC9D2C" modified="2009-07-28" version="1">MSIQTSDSSSNDYGKALSLIAAHSHISGLGLDENLQPKATSQGMVGQSQARRAAGVILKMVQSGTIAGRAVLIAGPPSTGKTALAMGLSQSLGQDVPFTAIAGSEIFSLELSKTEALTQAFRKSIGVKIKEETELIEGEVVEIQIDKSITGGHKQGKLTIKTTDMETIYELGSKMIEGLTKEKVLAGDVISIDKASGKITKLGRSFARSRDYDAMGADTRFVQCPEGELQKRKSVVHTVSLHEIDVINSRTQGFLALFTGDTGEIRSEVRDQINTKVAEWKEEGKADIVPGVLFIDEVHMLDIECFSFINRALEDEFAPIVVMATNRGISRTRGTNYKSPHGLPLDLLDRSIIITTHNYNEQEIKTILSIRAQEEEVEVAPDALDLMTKIGLESSLRYASNLISVSQQIALKRKSNTVEIADIKRAYLLFLDSSRSVKFLQEYQPQYIDDEGKVQIASNMTGTDAMDTTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2F6</accession>
    <name>C5E2F6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">AP-3 complex subunit delta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0H04554g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Part of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Adaptor protein complex 3 (AP-3) is a heterotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Clathrin-coated vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the adaptor complexes large subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556079.1">
        <property type="nucleotide sequence ID" value="XM_002556033.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2F6"/>
    <dbReference type="FunCoup" id="C5E2F6">
        <property type="interactions" value="668"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2F6"/>
    <dbReference type="GeneID" id="8294392"/>
    <dbReference type="KEGG" id="lth:KLTH0H04554g"/>
    <dbReference type="eggNOG" id="KOG1059">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001908_1_1_1"/>
    <dbReference type="InParanoid" id="C5E2F6"/>
    <dbReference type="OMA" id="CIHTIII"/>
    <dbReference type="OrthoDB" id="10264595at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030123">
        <property type="term" value="C:AP-3 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030665">
        <property type="term" value="C:clathrin-coated vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000251">
        <property type="entry name" value="AP-3 complex subunit delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017105">
        <property type="entry name" value="AP3_complex_dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22781:SF12">
        <property type="entry name" value="AP-3 COMPLEX SUBUNIT DELTA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22781">
        <property type="entry name" value="DELTA ADAPTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037092">
        <property type="entry name" value="AP3_complex_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Clathrin/coatomer adaptor adaptin-like N-terminal" evidence="11">
        <location>
            <begin position="49"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="698"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="757"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="811"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="701"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="758"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="775"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="785"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="822"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="836"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="890"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="907"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="923"/>
            <end position="936"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR037092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR30217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="943" mass="107100" checksum="7EC2EB633CAC3122" modified="2009-07-28" version="1">MTSLYAPSADDVMRRLRPFGLFFEKSLKDLIKGIRSCGENSEELERFLSKTLSECRQEVESPDLDLKTNAVVKLTYLEMYGFDMSWANFYILEVMSSNRLQHKRVGYLAASQSFHKDPDILMLATNLLKKDLKYDGNNDVLKMGVTLSGLSTMVTAPLARDICGDLFSMLGSSKPYIRKKAISALFKVFLQYPEALRDNFDKFVAKLEDEDMSVVSATVSVICELSKKNPHPFVQLSPLLYETLFTIDNNWIIIRLLKLFTNLSQVEPKLRVKVLPKILELMEVTSATSVIYESINCIVKGHMLEPDDYDTALSCLEELTKFCNSNDPNLRYISVVLFYKIGKINTSFISEFNTLVIRLLKDVDISIRSRALELLEGITDDENIAQIVQILVKQFADKDVVLANKLFKQTRQENIEIEVPNSYKIKMVSTILRICSLNNYANVPDFDWYLAVLSDLCVISQDLNDEAIGLQLGAELRNIMVKVPSMRETCISTIVGLVSNNDICRQLPMVLKECLWCIGEYPSSISNGDDIIKLITRQRRLTPEVQQIAAQALIKIFSSWCNTNGNVPINDVKHTIQELISFFESLSSSKSFEVQERCVEFLEFFKLCAESLEQNDEELPLLITEVLPSFFNAYELNPILHGTLKKVQNSLSIDLETPFLSEDEVKNLLEEESSKHEAESMLASDLDMDDEFEEDDAIENPAHEDKLLGDRSRDDLAEEERRVLEEERRKEKLTNPFYLEGDYEDFAAKRENPLIDFNDNQDQNYPESSSLIAGELKHVSETSKKTKKERKKKKKKAEVLIDEGVPEITSDVAAIPEPQPAKSISSSKNSSRINLKMQTKLESFNFDQPQKYVEDSGAAGTEGQQEIDQLRAKFAESSLGQDMDEEVVIVKRKKKKRSKDKKHSKKKDVEHHSAHGEPAKCDSGQNPQAENASAHDQTAHESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2L8</accession>
    <name>C5E2L8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TBP-associated factor 6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Transcription initiation factor TFIID subunit 6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0H06006g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556141.1">
        <property type="nucleotide sequence ID" value="XM_002556095.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2L8"/>
    <dbReference type="FunCoup" id="C5E2L8">
        <property type="interactions" value="1298"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2L8"/>
    <dbReference type="GeneID" id="8294456"/>
    <dbReference type="KEGG" id="lth:KLTH0H06006g"/>
    <dbReference type="eggNOG" id="KOG2549">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021711_3_1_1"/>
    <dbReference type="InParanoid" id="C5E2L8"/>
    <dbReference type="OMA" id="YFVQFIA"/>
    <dbReference type="OrthoDB" id="361039at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046695">
        <property type="term" value="C:SLIK (SAGA-like) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22931">
        <property type="entry name" value="HFD_TAF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08050">
        <property type="entry name" value="TAF6C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000033">
        <property type="entry name" value="Transcription initiation factor TFIID complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.770:FF:000003">
        <property type="entry name" value="Transcription initiation factor TFIID complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.770">
        <property type="entry name" value="TAF6, C-terminal HEAT repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037796">
        <property type="entry name" value="TAF6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011442">
        <property type="entry name" value="TAF6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046344">
        <property type="entry name" value="TAF6_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004823">
        <property type="entry name" value="TAF_TATA-bd_Histone-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10221">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10221:SF9">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02969">
        <property type="entry name" value="TAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07571">
        <property type="entry name" value="TAF6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00803">
        <property type="entry name" value="TAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TATA box binding protein associated factor (TAF) histone-like fold" evidence="9">
        <location>
            <begin position="10"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="317"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="324"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00076308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00093655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR30279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="511" mass="57416" checksum="7D33D14C69EFFEF9" modified="2009-07-28" version="1">MASQQQSYTIWSPQDTVKDVADSLGITNISDDVLRSLAMDVEYRILEIVEQAVKFKRHAKREVLTTEDIARALRVLNVEPLYGYQDGSARAKDASFSKVNAPGGQTLYYLNDEEVDFDKLINEPLPKVPRLPTFTTHWLAIEGVQPTIPQNPSLNDIRMSQPPIVRGAIVTTINDTSFQTSASDEKETQHISLIKPGQANEVKPLVKHVLSKELQIYFDKVVGALISKDRDDKAHHLRAAALTSLRSDTGLHQLVPYFIQFIAEQITHNLSDLDLLTTMLEMIYSLLSNTHVFLDPYIHSLMPSILTLLLAKKLGDNPPPNSSKEQREASEKVSKDTGTEFLEKTNALRDFAASLLDHVLRKFPQVYKSLKPRVMRTLLKTFLDTNRSFGTYYGCIKGVAVLGPESVRFFLGNLQSWANLVFEEHTTKENDKDEGGHTGRFTEQETKLLCDVIIESLVVLKSDLPPGQKYDEEITQSQKENLVKRCGSTIADRLLERSDASELVAAIFFGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2M2</accession>
    <name>C5E2M2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0H06094p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0H06094g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oligopeptide OPT transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556145.1">
        <property type="nucleotide sequence ID" value="XM_002556099.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2M2"/>
    <dbReference type="FunCoup" id="C5E2M2">
        <property type="interactions" value="34"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2M2"/>
    <dbReference type="GeneID" id="8294460"/>
    <dbReference type="KEGG" id="lth:KLTH0H06094g"/>
    <dbReference type="eggNOG" id="ENOG502QQ2H">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010539_2_0_1"/>
    <dbReference type="InParanoid" id="C5E2M2"/>
    <dbReference type="OMA" id="TPTAYVW"/>
    <dbReference type="OrthoDB" id="627262at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035673">
        <property type="term" value="F:oligopeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004813">
        <property type="entry name" value="OPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045035">
        <property type="entry name" value="YSL-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00728">
        <property type="entry name" value="OPT_sfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31645">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER YGL114W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31645:SF0">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER YGL114W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03169">
        <property type="entry name" value="OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="15"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="43"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="79"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="124"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="266"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="306"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="357"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="448"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="477"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="566"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="622"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="651"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="697"/>
            <end position="719"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR30283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="727" mass="79585" checksum="CE0BFC4FDA8576DB" modified="2009-07-28" version="1">MSEVPFEHKALTRQVSLRATIAGLAIGSLVLISNFQFGLQTGWVSMMSLPSALLACAIFKQVVPLFYPNAAVFTDVENVYVQSLAVAVGTGPLAYGFVGVIPAIENFLTAEESGYTRELGQRFSFQQLLLWSLALAFFGIFFAVPLRKQVIVREKLPFPSGSATATLIAVLNGSEILQEISPKELEEMRARRLREYSDVLANDEQTPLVEERVGNSAVEESEESNGGSSRTCLDFGCPAYEATPEPAPTAHCGSYERNISILSKTFLVSSSYTILSYFLPFMKAIPVFGQHLSKTYLWNLQPSPAYIGQGIIMGLPTVSYMVFGCVLGWAVLAPLARHKKWVDPKADINDWENGVQGWILWSSLAVMVVDSVVGFIVFTTKSLVQFCLRNDKRHLLESMIDDSVQSMLLEEERIINKGNGAGRRRVSTVKLVRTDMDHEVDHRQLVKFTTVVSGLVVSSIICIVCTLYLFGTEIVPLSSVIIALFVALFLSILAVRALGETDLNPVSGIGKLSQLIFTILMPHKKGAILLNLVAGGIAEAGAQQAGDLMQDLKTGHLLGASPKAQFFAQLIGASWSIVFSSVMHIFYNRIYEIPSSQFRVPTAVIWIDCARLVTGKGLPDKALECTIILGSIFAILSLIKNLYRDRGHKWLLWIPSGVAVGVGIYNAPSFTLARFIGGCTAQYWLRTHGSDSSAKTCMIVFSSGLVLGEGICSIFNMIFTSYNVPHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2Q9</accession>
    <name>C5E2Q9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">glucan 1,3-beta-glucosidase</fullName>
            <ecNumber evidence="9">3.2.1.58</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0H06974g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>Successive hydrolysis of beta-D-glucose units from the non-reducing ends of (1-&gt;3)-beta-D-glucans, releasing alpha-glucose.</text>
            <dbReference type="EC" id="3.2.1.58"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.58" evidence="9"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556182.1">
        <property type="nucleotide sequence ID" value="XM_002556136.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2Q9"/>
    <dbReference type="SMR" id="C5E2Q9"/>
    <dbReference type="FunCoup" id="C5E2Q9">
        <property type="interactions" value="176"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2Q9"/>
    <dbReference type="GeneID" id="8294499"/>
    <dbReference type="KEGG" id="lth:KLTH0H06974g"/>
    <dbReference type="eggNOG" id="ENOG502QPYU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004624_0_1_1"/>
    <dbReference type="InParanoid" id="C5E2Q9"/>
    <dbReference type="OMA" id="MDYHEYQ"/>
    <dbReference type="OrthoDB" id="62120at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004338">
        <property type="term" value="F:glucan exo-1,3-beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009251">
        <property type="term" value="P:glucan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000033">
        <property type="entry name" value="Glucan 1,3-beta-glucosidase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018087">
        <property type="entry name" value="Glyco_hydro_5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050386">
        <property type="entry name" value="Glycosyl_hydrolase_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31297:SF1">
        <property type="entry name" value="GLUCAN 1,3-BETA-GLUCOSIDASE I_II-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31297">
        <property type="entry name" value="GLUCAN ENDO-1,6-BETA-GLUCOSIDASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00150">
        <property type="entry name" value="Cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00659">
        <property type="entry name" value="GLYCOSYL_HYDROL_F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6 10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950732" description="glucan 1,3-beta-glucosidase" evidence="11">
        <location>
            <begin position="20"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="12">
        <location>
            <begin position="91"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00036824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR30320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="442" mass="50673" checksum="9F0F49F601CC3E80" modified="2009-07-28" version="1">MLALTWVYTLSVAVCAALAQPVPAKGPHSVQFMHEQNKKRFYDYSSATIRGVNIGGWFVLEPFITPSLFEVFRTIDNNDEGIPADEYHYCQALGQEVAASRLEQHWSSWFTEKDFANIADSGLNFVRIPIGYWAFKTLESDPYVTGKQEYYLDQAIGWARNNGLKVWVDLHGAAGSQNGFDNSGLRDSYAFLEDSNLALTKEVLQYLLEKYSRDEYLDTVIGVELINEPLGPVLDMDKLKEYYQFGYDYLRNELGRDQIVIIHDAFEPYNYWDDTLVYNQGAWGVVVDHHHYQVFSNKELLRSIDEHVSVACEWGSGTLSEAHWTVCGEWSAALTDCAKWLNGVGYGHRYDGTFHKGDDTSSYIGSCENNEDINSWSDERKADTRKYVEAQLDAFELRGGWVIWTYKTETSLEWDLQRLMFNGLFPQPVTDRQYPNQCGFSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2T5</accession>
    <name>C5E2T5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0H07568p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0H07568g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556208.1">
        <property type="nucleotide sequence ID" value="XM_002556162.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2T5"/>
    <dbReference type="FunCoup" id="C5E2T5">
        <property type="interactions" value="121"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2T5"/>
    <dbReference type="GeneID" id="8294526"/>
    <dbReference type="KEGG" id="lth:KLTH0H07568g"/>
    <dbReference type="eggNOG" id="ENOG502S5CB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116829_1_0_1"/>
    <dbReference type="InParanoid" id="C5E2T5"/>
    <dbReference type="OMA" id="EQRQYKI"/>
    <dbReference type="OrthoDB" id="4067384at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR30346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="155" mass="17724" checksum="57FE312E0D06B204" modified="2009-07-28" version="1">MWWLSRACFVCDDYYVTPSPRTVPLPQLLSWPTSSHQPPLTVIHTSEPPPPMDPQEPRHAQYKVQLLLHINSVLLARVNQLSYNTAHFSPEQQQNIVSQYLKRVHANLQCISQLNQGHAGCKPAILEPPQLPSQQPSQDILAKLYLLTSRVFEVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2V6</accession>
    <name>C5E2V6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0H08030p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0H08030g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556229.1">
        <property type="nucleotide sequence ID" value="XM_002556183.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2V6"/>
    <dbReference type="FunCoup" id="C5E2V6">
        <property type="interactions" value="30"/>
    </dbReference>
    <dbReference type="GeneID" id="8294547"/>
    <dbReference type="KEGG" id="lth:KLTH0H08030g"/>
    <dbReference type="eggNOG" id="ENOG502S6BB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125085_0_0_1"/>
    <dbReference type="InParanoid" id="C5E2V6"/>
    <dbReference type="OrthoDB" id="4069967at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16588">
        <property type="entry name" value="zf-C2H2_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="103"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR30367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="150" mass="16451" checksum="1B2BA49E67B42016" modified="2009-07-28" version="1">MGSQAQDPEQVSRAMDALARSILAKRVQDHKRKPERLRQLELLKQQFDALVLARQAQKEPAPRAPLLDAAQLDRELEQGLGFVERNGRNYVLLPLVGNPNPAPAAAPGATPAAPKHAKKKKNKIPCSYCHEAGHTRARCEKRLLGESGGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>C5E367</accession>
    <name>C5E367_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">3-ketodihydrosphingosine reductase TSC10</fullName>
            <ecNumber evidence="14">1.1.1.102</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">3-dehydrosphinganine reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">KDS reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ordered locus">KLTH0H10780g</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Catalyzes the reduction of 3'-oxosphinganine (3-ketodihydrosphingosine/KDS) to sphinganine (dihydrosphingosine/DHS), the second step of de novo sphingolipid biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>sphinganine + NADP(+) = 3-oxosphinganine + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22640"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57817"/>
            <dbReference type="ChEBI" id="CHEBI:58299"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.102"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="19">
            <dbReference type="Rhea" id="RHEA:22642"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.102" evidence="14"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556340.1">
        <property type="nucleotide sequence ID" value="XM_002556294.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E367"/>
    <dbReference type="FunCoup" id="C5E367">
        <property type="interactions" value="140"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E367"/>
    <dbReference type="GeneID" id="8294671"/>
    <dbReference type="KEGG" id="lth:KLTH0H10780g"/>
    <dbReference type="eggNOG" id="KOG1210">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_3_0_1"/>
    <dbReference type="InParanoid" id="C5E367"/>
    <dbReference type="OMA" id="PRQWGFF"/>
    <dbReference type="OrthoDB" id="10267115at2759"/>
    <dbReference type="UniPathway" id="UPA00222"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047560">
        <property type="term" value="F:3-dehydrosphinganine reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006666">
        <property type="term" value="P:3-keto-sphinganine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08939">
        <property type="entry name" value="KDSR-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000578">
        <property type="entry name" value="3-ketodihydrosphingosine reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045022">
        <property type="entry name" value="KDSR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43550">
        <property type="entry name" value="3-KETODIHYDROSPHINGOSINE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43550:SF3">
        <property type="entry name" value="3-KETODIHYDROSPHINGOSINE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="5 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="279"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00026112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00026241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00029797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00032891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00044737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="CAR30478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="314" mass="35088" checksum="6B44F2A9A4DBF86A" modified="2009-07-28" version="1">MAKFSLENQVALIAGGSQGLGKEFARKLYQEGTNNTVIVVSRSQDKLQRAVCSITGLETALELSEDARNLQHEQQQNRIFFASCDLAEYAQVEKLYELLAACEIVPTQVFLCAGGSTPGLFKDLSGRELDLGVRVNYMTCVNLAHIAVRKWPGSHLVFFSSEVAFFPFIGYSQYAPLKQSIKSLVAILRQECAPQRISCVYPGNFDSEGYALENETKPTITKEIEGPSDAISCAECCDRICKTLKAGYDDVTTDFIGWVLMSTDMGLNKQNNVSFGWPLQWLLGIFANLIIVPIYMLICKFQIRQWRKSCQKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3A5</accession>
    <name>C5E3A5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0H11704p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0H11704g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
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        <property type="protein sequence ID" value="CAR30516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome H"/>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
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    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
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    </dbReference>
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        <property type="entry name" value="OAF3"/>
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        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
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    <dbReference type="PANTHER" id="PTHR31069">
        <property type="entry name" value="OLEATE-ACTIVATED TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069:SF12">
        <property type="entry name" value="TRANSCRIPTION FACTOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="8">
        <location>
            <begin position="11"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="203"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="982"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1044"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="203"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="226"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="982"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1044"/>
            <end position="1054"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR30516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1185" mass="134357" checksum="F2F9CBF3BF777F7C" modified="2009-07-28" version="1">MSGPKIRAGGSCLVCRRRKVKCDRTKPVCLACIKYKSDSACDYETKRNVRFLHMVYSEPTGNIDGENKVTKPQLQKPNTNVSNVGGSNCNMTFKKTKGVEASEQRLPTQLLAFGHMSFYDGYEAVEIKASRLLFTGALHSIAVLRRDPFLYVLVAMIRKDRSKLHERISKAEPSSSRKYPVPLYTQEEIASQLIINARANHEHPCSNSVDDCRRYSRSGRSQNDSKISESLKLEPQKPGFPSQGKLTSADISTDTKFQQKLIENEGLDEVKSVVEEGNLPRNPLSVVNLLNEDSQAKSSLANSSTQNNDKNKDIEIWRKLQSMLATLQQRQHRLMRQLEPSRKNDLNAITSASFLGAMPSPEMSVLAKVREILPPTKIIWLHLRNYFRSPLHALFPVLTEDWFCDTVKEFIKNHKDDMSKPEVVVTQRCDFGKIGCMLIIMRLSFLMYADKAEKNLPEEQRDVYAHSIGPEFISTAELCLSLFRLLRKAILPVLHCAVLLRIYRRYAPDEGDIADSGGSEPFSGILTKMATSIGLHVDAEFSGQFAFSKMYSQAWRKCWYVIYFLDLYEGMNSGNATTINEHSFSTRLPVVGTNEDGTLSSYITVPELEIASIECLRKNYDFSIACHKLLNLVMNRNFKVSCEKVQSAAGELELLLKKTYEKGLRKILKHPAKTIDESITKCNDFKVFIETYSFLFMVYFHLFLHVDKLVIGDPDRCPPSTSFHYLQKLLEIYVELEPALVVLRASKDYHGNDSEFFDTLFGNNSKLVVIQSCGMFIVRFTTILQCLAARLLHLKYNFLLRPSPYRLAGEEREVPGVSEAADELITLLLKKLSIINEVTQSLSETYFYAWRISKGNSYIYDLLMAEDSILDHKSNISRSYRAACKDEPAFNSEKPMPFVNFYDVIPESNSLLHAKVSDLKKLLFILQSDDGNLLSSLTHRSHSFVTENTPLSAFQSPLSKFEEPGQNALSLIGMSSIKKATDASSDPNTFTSLASPTSFRVEGTSSGSNSLNSQKMEKSRSYVNSEDIDTFWYKIKLRNANMSAERSFSQSSGPPNRPNASDEKSKIFSSSWHNNNHNPPSPTERLVRNELHDTGHFNEIFNNQSLLQEHVMQQLQQLTEEQPIPQNPSRQGEYVESFTPHLAPNHREDKRNIEGSFLNNNSLEAGDFDMFLPTFDAFIEDIDFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3A7</accession>
    <name>C5E3A7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">diphthine methyl ester synthase</fullName>
            <ecNumber evidence="4">2.1.1.314</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0H11748g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">S-adenosyl-L-methionine-dependent methyltransferase that catalyzes four methylations of the modified target histidine residue in translation elongation factor 2 (EF-2), to form an intermediate called diphthine methyl ester. The four successive methylation reactions represent the second step of diphthamide biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>2-[(3S)-amino-3-carboxypropyl]-L-histidyl-[translation elongation factor 2] + 4 S-adenosyl-L-methionine = diphthine methyl ester-[translation elongation factor 2] + 4 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:42652"/>
            <dbReference type="Rhea" id="RHEA-COMP:9749"/>
            <dbReference type="Rhea" id="RHEA-COMP:10173"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73995"/>
            <dbReference type="ChEBI" id="CHEBI:79005"/>
            <dbReference type="EC" id="2.1.1.314"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the diphthine synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.314" evidence="4"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556380.1">
        <property type="nucleotide sequence ID" value="XM_002556334.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3A7"/>
    <dbReference type="FunCoup" id="C5E3A7">
        <property type="interactions" value="995"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3A7"/>
    <dbReference type="GeneID" id="8294714"/>
    <dbReference type="KEGG" id="lth:KLTH0H11748g"/>
    <dbReference type="eggNOG" id="KOG3123">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066040_1_0_1"/>
    <dbReference type="InParanoid" id="C5E3A7"/>
    <dbReference type="OMA" id="HNASIMS"/>
    <dbReference type="OrthoDB" id="2516at2759"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141133">
        <property type="term" value="F:diphthine methyl ester synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd11647">
        <property type="entry name" value="DHP5_DphB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.950.10:FF:000004">
        <property type="entry name" value="Diphthine synthase putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1010.10:FF:000004">
        <property type="entry name" value="Putative diphthine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="entry name" value="Cobalt-precorrin-4 Transmethylase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="entry name" value="Methyltransferase, Cobalt-precorrin-4 Transmethylase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01084">
        <property type="entry name" value="Diphthine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004551">
        <property type="entry name" value="Dphthn_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00522">
        <property type="entry name" value="dph5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10882:SF0">
        <property type="entry name" value="DIPHTHINE METHYL ESTER SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10882">
        <property type="entry name" value="DIPHTHINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036432">
        <property type="entry name" value="Diphthine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="Tetrapyrrole methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tetrapyrrole methylase" evidence="10">
        <location>
            <begin position="1"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="113"/>
            <end position="114"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR036432-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR30518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="299" mass="33911" checksum="1DAB6A0B66FF3432" modified="2009-07-28" version="1">MLYLIGLGLSYTTDITVRGLEAIKKCDRIYLEHYTSILMAASLEELEQFYGKPVTLADRELVESGCEEILRDADKQDVAFLVVGDPFGATTHTDLVLRAKRQGLPVEVIHNASVMNAVGSCGLQLYTFGQTVSMVFFTDDWRPDSWYNKIWENRKIGLHTLVLLDIKVKEQSIENMARGRLIYEPPRYMSISQCCEQLLEIEETRGTKAYTPDTPVVAISRLGSASQEFKSGTIEELSRYDAGEPLHSLVILGRQCHELELEYLLDFASDREAFKQAVIKDQEYFKPAPWVPPAEEDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3C9</accession>
    <name>C5E3C9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0H12298p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0H12298g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556402.1">
        <property type="nucleotide sequence ID" value="XM_002556356.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3C9"/>
    <dbReference type="FunCoup" id="C5E3C9">
        <property type="interactions" value="459"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3C9"/>
    <dbReference type="GeneID" id="8294739"/>
    <dbReference type="KEGG" id="lth:KLTH0H12298g"/>
    <dbReference type="eggNOG" id="KOG3038">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023535_2_0_1"/>
    <dbReference type="InParanoid" id="C5E3C9"/>
    <dbReference type="OMA" id="VGRSYWT"/>
    <dbReference type="OrthoDB" id="10265994at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20393">
        <property type="entry name" value="Tudor_SGF29_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20394">
        <property type="entry name" value="Tudor_SGF29_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000055">
        <property type="entry name" value="SAGA complex component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037802">
        <property type="entry name" value="SGF29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010750">
        <property type="entry name" value="SGF29_tudor-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002999">
        <property type="entry name" value="Tudor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047288">
        <property type="entry name" value="Tudor_SGF29_rpt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047287">
        <property type="entry name" value="Tudor_SGF29_rpt2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21539">
        <property type="entry name" value="SAGA-ASSOCIATED FACTOR 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21539:SF0">
        <property type="entry name" value="SAGA-ASSOCIATED FACTOR 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07039">
        <property type="entry name" value="SGF29_Tudor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00333">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51518">
        <property type="entry name" value="SGF29_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="SGF29 C-terminal" evidence="6">
        <location>
            <begin position="111"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="94"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR30540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="246" mass="28030" checksum="C249266FE03406B5" modified="2009-07-28" version="1">MDDQWDVVVSSLQELLKKHETTSFDDLVNEKRLNFHNLSSEQLLTQLESFTTHSQNLEKAIELIETISNNLNLVIKYVQENTESAEAAETKTPSSSESYSGRSYWTSPYNPSEPIVLESEVAYRPKKGGDGEWFQCQVIKISSDGTRFEVRDPEPDELGNPGQMYKCNWKDVILIPARSAPKHQTPNYPSGTKVLARYPETTTFYPAVVIGHKRDGTCKLRFDGEEEVDKETEVARRLVLPFPARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3J5</accession>
    <name>C5E3J5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Asparagine--tRNA ligase, mitochondrial</fullName>
            <ecNumber evidence="2">6.1.1.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Asparaginyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0H14080g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.22" evidence="2"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556468.1">
        <property type="nucleotide sequence ID" value="XM_002556422.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3J5"/>
    <dbReference type="FunCoup" id="C5E3J5">
        <property type="interactions" value="519"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3J5"/>
    <dbReference type="GeneID" id="8294830"/>
    <dbReference type="KEGG" id="lth:KLTH0H14080g"/>
    <dbReference type="eggNOG" id="KOG0554">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004553_2_0_1"/>
    <dbReference type="InParanoid" id="C5E3J5"/>
    <dbReference type="OMA" id="PEMAFYD"/>
    <dbReference type="OrthoDB" id="43906at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004816">
        <property type="term" value="F:asparagine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006421">
        <property type="term" value="P:asparaginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00776">
        <property type="entry name" value="AsxRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04318">
        <property type="entry name" value="EcAsnRS_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000016">
        <property type="entry name" value="Asparagine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004522">
        <property type="entry name" value="Asn-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00457">
        <property type="entry name" value="asnS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003037">
        <property type="entry name" value="PRK03932.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594:SF34">
        <property type="entry name" value="ASPARAGINE--TRNA LIGASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594">
        <property type="entry name" value="ASPARTYL/LYSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="10">
        <location>
            <begin position="142"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00068798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR30606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="468" mass="52966" checksum="D9E8BF9E0856C78C" modified="2009-07-28" version="1">MPGLPPTFKSLFRNASNPPAKVELLCGWIKSVRLLKKLAFIDLQDGTTPNPLKLVVRRSEDGSDMLKQLRTGQSLMVKDALWKATPSRLQPFELETDASPECLQVLGQVPASYPLQKKHHSLAFLRSNPTLKHRSNYLGSLLRFRSCVESSLTQFFDSQDFTKVSPPVLTSGDCEGAGELFRAESNAHIARKTSYFGRPTYLTVSTQLHLEVLAMALNRVWTLTPCFRAEESDTNRHLAEFWMLETELCYVQNVKGLTKVAEHMLKHVVESCLAQKLSLLPSVVPQENAESPESIVARWESLLHSEWPRVTYTSAIELLKRQHSVSQFSFEPQWGKDLQSEHEKWLAAHYNSPVFITDYPRNCKPFYMKQNFDAADTVACFDLIVPQMGEIIGGSIREDNYDTLIQEMDRRGMKSEDLDWYTSLRLNGGAPHGGFGLGLERFVSWLFGAQNIRDAIPFYRSAGGTINM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3N5</accession>
    <name>C5E3N5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">Protein phosphatase methylesterase 1</fullName>
            <shortName evidence="6">PME-1</shortName>
            <ecNumber evidence="6">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0H15026g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Demethylates proteins that have been reversibly carboxymethylated.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>[phosphatase 2A protein]-C-terminal L-leucine methyl ester + H2O = [phosphatase 2A protein]-C-terminal L-leucine + methanol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48548"/>
            <dbReference type="Rhea" id="RHEA-COMP:12134"/>
            <dbReference type="Rhea" id="RHEA-COMP:12135"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17790"/>
            <dbReference type="ChEBI" id="CHEBI:90516"/>
            <dbReference type="ChEBI" id="CHEBI:90517"/>
            <dbReference type="EC" id="3.1.1.89"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the AB hydrolase superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="6"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556508.1">
        <property type="nucleotide sequence ID" value="XM_002556462.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3N5"/>
    <dbReference type="FunCoup" id="C5E3N5">
        <property type="interactions" value="886"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3N5"/>
    <dbReference type="ESTHER" id="lactc-c5e3n5">
        <property type="family name" value="PPase_methylesterase_euk"/>
    </dbReference>
    <dbReference type="GeneID" id="8294872"/>
    <dbReference type="KEGG" id="lth:KLTH0H15026g"/>
    <dbReference type="eggNOG" id="KOG2564">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024818_3_0_1"/>
    <dbReference type="InParanoid" id="C5E3N5"/>
    <dbReference type="OMA" id="VMVCHHG"/>
    <dbReference type="OrthoDB" id="194865at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051723">
        <property type="term" value="F:protein methylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016812">
        <property type="entry name" value="PPase_methylesterase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14189:SF0">
        <property type="entry name" value="PROTEIN PHOSPHATASE METHYLESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14189">
        <property type="entry name" value="PROTEIN PHOSPHATASE METHYLESTERASE-1 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF022950">
        <property type="entry name" value="PPase_methylesterase_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0719">Serine esterase</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="8">
        <location>
            <begin position="83"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="173"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="201"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR022950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR022950-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR30646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="365" mass="39875" checksum="8FD0DE8923A9946F" modified="2009-07-28" version="1">MSDDLRRALLEKLGKADAQLGAASQPAPAAPAPAGLPTWHDFFQQNARVQIGDRGLDFNTYFSLPAASELADPSNTTSVPIFIFHHGAGSSGLSFAPLAKALHEQLDGRCGSFAFDARGHGLTAPVDAAAHPADYSLDAFVRDFRELIAWFHHTHLQALPASPTFTLVITGHSLGGSICTNVFPSLDPALRTKVAGLAMLDIVEEAAILALSKVDSFLSNTPNVFPSYANAIDWHVKRGLSRLRSSAEIVVPSLFVPTSSGKVVRRTNLQIFKPFWDTWFQSLSRRFVALPTSKLLILAGNDNLDRELMIGQMQGKFQLVVFQESGHFIEEDAPVKTAITLVDFWRRNDTKSVVIKTNWTKKANT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBJ4</accession>
    <name>C5DBJ4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0A03124p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0A03124g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551593.1">
        <property type="nucleotide sequence ID" value="XM_002551547.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBJ4"/>
    <dbReference type="GeneID" id="8290393"/>
    <dbReference type="KEGG" id="lth:KLTH0A03124g"/>
    <dbReference type="HOGENOM" id="CLU_1390467_0_0_1"/>
    <dbReference type="InParanoid" id="C5DBJ4"/>
    <dbReference type="OrthoDB" id="4036656at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="153"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="196" mass="21634" checksum="8B49A5100E674E8B" modified="2009-07-28" version="1">MIFAHTDLDFQETSMGLARSYPSLDAGSLSEGAEQEQQPRAPIEDVLELIISGTESELDPPIFEAELLTSDMAVDGLRQDSIFSLDGDADLSLPDLQETESFDTSLEQLPYEAFNACADQPAETPGAPARPRLSHRKSNPFYLPSKHIKNMISKDRHRQSARKVESASRSQWGGAGEALIPDQCTKPFLERRQTIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DC14</accession>
    <name>C5DC14_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0A07018p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0A07018g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS12 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551763.1">
        <property type="nucleotide sequence ID" value="XM_002551717.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DC14"/>
    <dbReference type="FunCoup" id="C5DC14">
        <property type="interactions" value="408"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DC14"/>
    <dbReference type="GeneID" id="8290570"/>
    <dbReference type="KEGG" id="lth:KLTH0A07018g"/>
    <dbReference type="eggNOG" id="KOG1750">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104295_0_1_1"/>
    <dbReference type="InParanoid" id="C5DC14"/>
    <dbReference type="OMA" id="VCIRVYT"/>
    <dbReference type="OrthoDB" id="361013at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03368">
        <property type="entry name" value="Ribosomal_S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000099">
        <property type="entry name" value="Ribosomal protein S12, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006032">
        <property type="entry name" value="Ribosomal_uS12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005679">
        <property type="entry name" value="Ribosomal_uS12_bac"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00981">
        <property type="entry name" value="rpsL_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11652">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S12 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00164">
        <property type="entry name" value="Ribosom_S12_S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01034">
        <property type="entry name" value="RIBOSOMALS12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00055">
        <property type="entry name" value="RIBOSOMAL_S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR21321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="171" mass="18894" checksum="B004313B31C2DDB5" modified="2009-07-28" version="1">MLFPSLTSRFVLGLSRASMIPRAPARSSAMAIFSPLRAFSICRPNWATLNQIKRGVEPPKRKKATVSPDLYQSPLRKGVVLRVMVLKPKKPNSAQRKACRVRLTNGKVISAYIPGEGHNAQEHSIVYVRGGRCQDLPGVKYRVIRGTGDLSGVVNRITSRSKYGVKKPQKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5DC50</accession>
    <name>C5DC50_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0A07832p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0A07832g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551799.1">
        <property type="nucleotide sequence ID" value="XM_002551753.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DC50"/>
    <dbReference type="GeneID" id="8290607"/>
    <dbReference type="KEGG" id="lth:KLTH0A07832g"/>
    <dbReference type="eggNOG" id="KOG2533">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_0_5_1"/>
    <dbReference type="InParanoid" id="C5DC50"/>
    <dbReference type="OMA" id="YCAGCIG"/>
    <dbReference type="OrthoDB" id="4454541at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF103">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="97"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="156"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="193"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="289"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="332"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="359"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="383"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="418"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="444"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR21357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="504" mass="57123" checksum="1FD4FB23C7C0B79E" modified="2009-07-28" version="1">MSEEEKPVQRGTIEEAEISSRSSSLIEKPDKITNEDALNDEYLETVTFTAEEEKALVRKIDLYILPLMCFVFLSQYLDKQSLSYATIFGLTKDLKMGQHDISWCSTIFSIGQLGANFVFTYLMTIVPRAPMTGACVVIWSVCCMCLAAPTTKEGFWVGRLFLGIFEAVVQPACVLITSYWYRKREQPLRTACWISMNAIAQIVGSFLMYGIGKTNKSSVADWKLMFIACGVISLVAGIAFYLLIPVSPRTAWFLTKREKEIAVKRLFDESDRTATNKFKKDQLFECFKFDWLFLSSLAFGFLVTVTSGTIIFQSLMLFSFGYDKFEVMKYGSPAGAVQLLFIWVAVVAVKLFPRERAMISLALVCVPLAGSIMVLSLKQSSGWWIIVGSWLGSVISCIMSILLSLISSNVRGNTKKSVCSNAFFAGYCLAAIIYPQWWVGTYKGGLIVNIIMWIIMDIYLIFYRFKAIFENKKRDKLQEEGFTLETHLVDDLTDKQNIHHRYVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCD0</accession>
    <name>C5DCD0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0B02024p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0B02024g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551879.1">
        <property type="nucleotide sequence ID" value="XM_002551833.1"/>
    </dbReference>
    <dbReference type="SMR" id="C5DCD0"/>
    <dbReference type="STRING" id="559295.C5DCD0"/>
    <dbReference type="GeneID" id="8290707"/>
    <dbReference type="KEGG" id="lth:KLTH0B02024g"/>
    <dbReference type="eggNOG" id="KOG3687">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003828_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCD0"/>
    <dbReference type="OMA" id="WISTAID"/>
    <dbReference type="OrthoDB" id="19311at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033596">
        <property type="term" value="C:TSC1-TSC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032007">
        <property type="term" value="P:negative regulation of TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051056">
        <property type="term" value="P:regulation of small GTPase mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11210:FF:000007">
        <property type="entry name" value="Tuberous sclerosis 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11210">
        <property type="entry name" value="Rap/Ran-GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035974">
        <property type="entry name" value="Rap/Ran-GAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000331">
        <property type="entry name" value="Rap/Ran_GAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018515">
        <property type="entry name" value="Tuberin-type_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027107">
        <property type="entry name" value="Tuberin/Ral-act_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024584">
        <property type="entry name" value="Tuberin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10063">
        <property type="entry name" value="TUBERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10063:SF0">
        <property type="entry name" value="TUBERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11864">
        <property type="entry name" value="DUF3384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02145">
        <property type="entry name" value="Rap_GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03542">
        <property type="entry name" value="Tuberin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111347">
        <property type="entry name" value="Rap/Ran-GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50085">
        <property type="entry name" value="RAPGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rap-GAP" evidence="2">
        <location>
            <begin position="1162"/>
            <end position="1391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR21441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1413" mass="158311" checksum="4669A7FBA3997224" modified="2009-07-28" version="1">MAPPLIYKAYTSGLKRELFRTLSGNFKSTPTPSNYEQCAVTKEPLHAQKLIPLIADRASGYSSRARLVKEIIPELSFLSAESVLELWDGANDLIAPEIPSSTRAYGLDLLHACIERLLPVMSETLRLAFFYNIMDNCGVADEKAGKAEKELHKVIKSLDALTCHGTRLESILRADESTCGLEDFFESIFASISVGGEPKPPHQTVVSALRFAESCARHGVPITDELISCVVTISRGWDENEVRKGALDLLKAAFSERPVESKELSRSVLQALLTIYHENNELSDSVTETLHLALSGQNGTQLLPLFLDMGFFDGGNTDVSYISLLVSLLTVDNVEGLWQKCGSSPRHIAELLLRLLRQEFNKHSSSNISASQLFGNILCLLESEEFLRWISKFRGFWLVSEEEPSIQDLLTPFLQKVELDDLDVKYLRMIFNQILNISQSGNYKLFGINEFKDIFEFLFAYSRHLNDSLANQLLDLLDQSTSDAVLVDGLVHDIITRVYLADFGTNVRCRTLEVIEKCCYEIISKRKQWDSSLCRSLKHLFSMLETEKDNTVALKQIEIYCETCRYLPSGFIAAVNSEVFGQAFTKPSSRRRSSLIPLSLGSSPAPAWSEVKLIKLTEGLAGLLVWCIAFERGDCFGSFYRLLTKAAQYAYQTAQSELFLASARVLSKVYCGNSRQVVFQETNDVEGITIALNKNSRAASTLENNGWSYPETIIYFNEAQSKVLKCDAYSPELVKDDFVTPGAVDIRTWISTAIDMIGNPTTWEVYSYLLTFMCPQFANLVALRSIDDLVGLYRDVICRHMKSGLPSKLKIPSGLCTDNLHAVYVRSLSPLLAYHSYLPKDFTDGIVESLLYGLHTKDSTLTLSLHLLTICCQEIPGSVKKLLTPILVQLQTRVTSSMCTPAILEFLLALSNSPFITSHLTADEFKRVFAIAFKFIQSSRDLKERAQTQNIIQQISYLEQAADSSPSTHSFKITASVAHFFLTLSYKVISSWFLRMKLSNRVELAPFVVKCLVSSASEAESKDYDVSAHIDIISHLTATPHGPSFPHRINEDRANESNDGYDFGRWILSDKIVSIETDIQTGDSVITMRSPSSSEVFKLQFERSQIPKPYDIFRIGQEEEEEPSTEKFEQFKFTASFVLTQLGCVGRHPVKVPDDSSIARSVHLFDKTPVSEYHKIGLIYVGPQQDSEAEVLSNTNGSYQYKLFLSRLGHLVKLKDCMNVYTGGLEPDVDGEFALVYNAEKTQAVFHAATLMPNNPQDDQYSMKKRHIGNNFVTIFFDESGSASFDFNIIKSQFNFINIVIKPYQATDTGKGSKHFKVRMYRKAGVPAFFSTSHFKILSAENLAKYVRHVSLIADTFSTCWFSASAPEVCTTWARRAKQMSSIREKTLKWYEGHGEPVSPPDFASVVEAAGGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCK8</accession>
    <name>C5DCK8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0B03872p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0B03872g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551957.1">
        <property type="nucleotide sequence ID" value="XM_002551911.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCK8"/>
    <dbReference type="FunCoup" id="C5DCK8">
        <property type="interactions" value="85"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCK8"/>
    <dbReference type="GeneID" id="8290791"/>
    <dbReference type="KEGG" id="lth:KLTH0B03872g"/>
    <dbReference type="eggNOG" id="ENOG502S031">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058662_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCK8"/>
    <dbReference type="OMA" id="PSKIMIG"/>
    <dbReference type="OrthoDB" id="4068255at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR21519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="362" mass="41452" checksum="671778AD39270C8E" modified="2009-07-28" version="1">MSLQKPLLDAIVESDHEGSVMVDHATDDSTSEVDESFVQLDGDEFRLDQEARRKGPVGKKLFVEEDSIAESPLRNGLLGPSLIASSPQKSDLESPSKRVQFKHHIDSASEEEPISEQPIDQWNFKDLIGDRFRERLPETDLKAWQRPSKRLLNSITDILENNIDLALEATFEKYEAECERLAIGRSVKHIRRDKERMLHKTIGKIERQLQSSKFPSRVKDRDFDIEYIYAKRTFIQNSFSQELEHTDSVEHQVLREQKALEDLKHVQAKLSNRALRQTKLLTEQLSHNLHPALSKAMVNSFGLIGDNAINKERYKKDVDELNLKLADPSAGVTHSLLHKYLPSLKNYEKTAEKLQGSLPNLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCM7</accession>
    <name>C5DCM7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="24">KLTH0B04356p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="24" type="ordered locus">KLTH0B04356g</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the multicopper oxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551976.1">
        <property type="nucleotide sequence ID" value="XM_002551930.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCM7"/>
    <dbReference type="FunCoup" id="C5DCM7">
        <property type="interactions" value="778"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCM7"/>
    <dbReference type="GeneID" id="8290813"/>
    <dbReference type="KEGG" id="lth:KLTH0B04356g"/>
    <dbReference type="eggNOG" id="KOG1263">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006504_7_3_1"/>
    <dbReference type="InParanoid" id="C5DCM7"/>
    <dbReference type="OMA" id="CDIAPGS"/>
    <dbReference type="OrthoDB" id="2121828at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033573">
        <property type="term" value="C:high-affinity iron permease complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004322">
        <property type="term" value="F:ferroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010106">
        <property type="term" value="P:cellular response to iron ion starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033215">
        <property type="term" value="P:reductive iron assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13851">
        <property type="entry name" value="CuRO_1_Fet3p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13877">
        <property type="entry name" value="CuRO_2_Fet3p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13899">
        <property type="entry name" value="CuRO_3_Fet3p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000022">
        <property type="entry name" value="FET5p Multicopper oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000024">
        <property type="entry name" value="FET5p Multicopper oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000025">
        <property type="entry name" value="FET5p Multicopper oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002355">
        <property type="entry name" value="Cu_oxidase_Cu_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044130">
        <property type="entry name" value="CuRO_2_Fet3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF361">
        <property type="entry name" value="IRON TRANSPORT MULTICOPPER OXIDASE FET3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00080">
        <property type="entry name" value="MULTICOPPER_OXIDASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0186">Copper</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0410">Iron transport</keyword>
    <keyword evidence="16 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 20" id="KW-0732">Signal</keyword>
    <keyword evidence="7 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950318" evidence="20">
        <location>
            <begin position="20"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="559"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="23">
        <location>
            <begin position="29"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="21">
        <location>
            <begin position="152"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="22">
        <location>
            <begin position="359"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="612"/>
            <end position="631"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="CAR21538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="631" mass="71196" checksum="98976440AD18D8AC" modified="2009-07-28" version="1">MIGSSWLLVMLAAVQSVAAETHVFNWTTGWGNYNVDGGAERPVITCNGEFPWPDVRVKKGDQVEVYLKNGFDDRNTSMHFHGLFQNGTNSMDGPPMVVQCPIGPGDTFLYNFTVDDNVGTYWYHSHTAGQYQDGMRGAFVIEDDDFPYDYDEDVILQLGEWYHDTTDVLNPKFMSVYNPTGAEPIPQNLIMNNTRNMTWEVQPDTTYLLRVINTGGFVSQYFWIEDHEFDVVEVDGIYTEKNTTDMIYITTAQRYTLLLHTKNDTSRNYAIMTKFDDTMLDLLPKDLQLNSTNYLIYNTSADKPTESYVDELAPLDDFYLVPYQKEEALGDPDYRVVVNVTMDNLVSGINYAFFNNLTYTAPVVPTLFTVLSADEDDVKNPEIYGSNINAFVLDKDEIVEIVLNNEDTGTHPFHLHGHAFQVMVRDVTYDDALGETPHAYDENDHPDFPEYPMIRDTLYVRPQSNFVIRFKANNPGVWMFHCHIEWHLTQGLAIILIEDPESIKATQSQQLSDNHKSVCANVGMATEGNAAGNTDDFFNLKGEPIQEKFIPDGFTAKGIVAMTFSCLAGVLGLIMIAIYGLMDISNVEEKVIDELHLDADKVAAVDVDDVDDIDNDETSSISAAREEVVNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCS9</accession>
    <name>C5DCS9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 11">RNA cytidine acetyltransferase</fullName>
            <ecNumber evidence="11">2.3.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">18S rRNA cytosine acetyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">NAT10</name>
        <name evidence="17" type="ordered locus">KLTH0B05522g</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">RNA cytidine acetyltransferase with specificity toward both 18S rRNA and tRNAs. Catalyzes the formation of N(4)-acetylcytidine (ac4C) in 18S rRNA. Required for early nucleolar cleavages of precursor rRNA at sites A0, A1 and A2 during 18S rRNA synthesis. Catalyzes the formation of ac4C in serine and leucine tRNAs. Requires the tRNA-binding adapter protein TAN1 for full tRNA acetyltransferase activity but not for 18S rRNA acetylation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 11">
            <text>a cytidine in 18S rRNA + acetyl-CoA + ATP + H2O = an N(4)-acetylcytidine in 18S rRNA + ADP + phosphate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:51424"/>
            <dbReference type="Rhea" id="RHEA-COMP:13575"/>
            <dbReference type="Rhea" id="RHEA-COMP:13576"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:74900"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a cytidine in tRNA + acetyl-CoA + ATP + H2O = an N(4)-acetylcytidine in tRNA + ADP + phosphate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53876"/>
            <dbReference type="Rhea" id="RHEA-COMP:13670"/>
            <dbReference type="Rhea" id="RHEA-COMP:13671"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:74900"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Interacts with TAN1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
            <location evidence="1 11">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the RNA cytidine acetyltransferase family. NAT10 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="11"/>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCS9"/>
    <dbReference type="FunCoup" id="C5DCS9">
        <property type="interactions" value="1308"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCS9"/>
    <dbReference type="KEGG" id="lth:KLTH0B05522g"/>
    <dbReference type="eggNOG" id="KOG2036">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004652_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCS9"/>
    <dbReference type="OMA" id="HLHYIMS"/>
    <dbReference type="OrthoDB" id="10067491at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990883">
        <property type="term" value="F:18S rRNA cytidine N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051392">
        <property type="term" value="F:tRNA cytidine N4-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904812">
        <property type="term" value="P:rRNA acetylation involved in maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051391">
        <property type="term" value="P:tRNA acetylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11040:FF:000002">
        <property type="entry name" value="RNA cytidine acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002218">
        <property type="entry name" value="tRNA(Met) cytidine acetyltransferase TmcA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03211">
        <property type="entry name" value="RNA_acetyltr_Nat10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033688">
        <property type="entry name" value="NAT10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007807">
        <property type="entry name" value="TcmA/NAT10_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032672">
        <property type="entry name" value="TmcA/NAT10/Kre33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013562">
        <property type="entry name" value="TmcA/NAT10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027992">
        <property type="entry name" value="tRNA_bind_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10925">
        <property type="entry name" value="N-ACETYLTRANSFERASE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10925:SF5">
        <property type="entry name" value="RNA CYTIDINE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13718">
        <property type="entry name" value="GNAT_acetyltr_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05127">
        <property type="entry name" value="NAT10_TcmA_helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08351">
        <property type="entry name" value="TmcA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13725">
        <property type="entry name" value="tRNA_bind_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="3 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 11" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="TmcA/NAT10 N-terminal" evidence="14">
        <location>
            <begin position="9"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="TcmA/NAT10 helicase" evidence="13">
        <location>
            <begin position="281"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="15">
        <location>
            <begin position="534"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="domain" description="Possible tRNA binding" evidence="16">
        <location>
            <begin position="771"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1011"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="286"/>
            <end position="295"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="473"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="636"/>
            <end position="638"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="643"/>
            <end position="649"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="737"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00052133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00068357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF05127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF13718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF13725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAR21590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1055" mass="118282" checksum="20203EB0CD319287" modified="2009-07-28" version="1">MAKKAIDSRIPSLIRNGAQTKQRSFFVIVGDKARNQLPNLHYLMMSADLKMNKSVLWAYKKKLLGFTSHRKKREAKIKKEIKRGTREVNEQDPFETFISNQNIRYVYYKETEKILGNTYGMCILQDFEALTPNLLARTVETVEGGGIVVILLKSMTSLKQLYTMTMDIHSRYRTEAHSDVVARFNERFILSLGSNENCLVVDDELNVLPISGGKNVQPLPPKDEDEIPPRQQELIELKESLEDVQPAGSLVALSKTVNQAQAILNFIDAISEKNLNSTVALTAGRGRGKSAALGIAIAAAVSHGYSNIFVTSPSPENLKTLFEFIFKGFDALGYQEHMDYDIIQSTNPSFNKAIVRVDIKKSHRQTIQYIVPNDSHVLGQAELVVIDEAAAIPLPVVKNLLGPYLVFMASTINGYEGTGRSLSLKLIQQLRDQANSSGRETSETMVVSRDNKKQDFHASGRSLKEVVLDEPIRYAPGDPVEKWLNKLLCLDVTLIKNPRFAARGTPHPSECNLFVVNRDTLFSYHPVSETFLEKMMALYVASHYKNSPNDLQLMSDAPAHQLFVLLPPINPKDGGRIPDPLVVIQVALEGEISRDSVKSSLSRGQRAGGDLIPWLISQQFQDEEFPSLSGARVVRVATNPEYSSMGYGSRAIELLRDYYEGKFADLNENSKPASHELKRVNDQELSKTNLLKDEVKLRDAKTLPPLLLKLSEQPPHFLHYLGVSYGLTSSLHKFWKKNKFVPVYLRQTANDLTGEHTCVMLNVLEGRESHWLSEYANDFHKRFLSLLSYEFRKFSAVQALNVIESVKKANGLSGNAENKHAARPLTKEDIDQVLSPFDLKRLDSYANNLLDYHVIVDLLPLLSSLYFSGRMGSSVNLSSVQSAILLAVGLQRKSVDDVSKELNLPFNQAIAMFAKIIRKFSIYFREVVSQSIERSMPEAKDDNIAEMNGEEVTALDASSVMEKMDDDLEKAGTRALTAMQEKQKELINSLNLDKYAIDDNAEEWAQSKKDLDKAAKKNGTVAVKSNKKRKTENAEDIFNAEMSAVKKSSKKKPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCY6</accession>
    <name>C5DCY6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0B06864p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0B06864g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRP1/MDG1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552085.1">
        <property type="nucleotide sequence ID" value="XM_002552039.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCY6"/>
    <dbReference type="STRING" id="559295.C5DCY6"/>
    <dbReference type="GeneID" id="8290925"/>
    <dbReference type="KEGG" id="lth:KLTH0B06864g"/>
    <dbReference type="eggNOG" id="KOG1616">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_323911_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCY6"/>
    <dbReference type="OMA" id="AMANQNP"/>
    <dbReference type="OrthoDB" id="5976022at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031588">
        <property type="term" value="C:nucleotide-activated protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02859">
        <property type="entry name" value="E_set_AMPKbeta_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032640">
        <property type="entry name" value="AMPK1_CBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050827">
        <property type="entry name" value="CRP1_MDG1_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10343">
        <property type="entry name" value="5'-AMP-ACTIVATED PROTEIN KINASE , BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10343:SF81">
        <property type="entry name" value="CRUCIFORM DNA-RECOGNIZING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16561">
        <property type="entry name" value="AMPK1_CBM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-activated protein kinase glycogen-binding" evidence="4">
        <location>
            <begin position="4"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="121"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="258"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="606"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="703"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="810"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="133"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="153"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="203"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="258"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="277"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="373"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="460"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="647"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="675"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="704"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="720"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="810"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="861"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="877"/>
            <end position="892"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR21647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="892" mass="94151" checksum="B4C4D19D36B37F79" modified="2009-07-28" version="1">MAEYTFRWPEGPQDVILTGDFDNWSGSLPLVKSPRGDFAITMPIPPSSKNKFHFKFIVDGQWMVSDNYDVDHSSEGIENNFLVVSSLPTPSSNKAAAAGGKIPEIGLNLVAPEVAAPVLGTQAPQKAQPAPRSNKKSKKKRIQIKRRIRRNKKTGERTVLSEERSELKSGTDDETYETEETATATNTSREDTPLSTDLDPLVNPTQRQNEFSSTVMPSTENRQTTLGEPGIAIVKDAAQIKEFSEIRDVDADELNERLNRELAEKQNREASTVEETPAEKETEPLKSADEGVHVSPPEFPVVEKNISPPEFPVVEKDLSSPAVKGGEEESSSKAPVAAFAAIPTGDHDVASESPVPESPTLKEESAPATLAAGKKDVKPLEASAAEEKGPKPALASGKQPVVAALPAPEKDTSSSKALVPENDDSSTRALAGDVSPAEVAAIATSPSALPVAEKEVEPSSAVSEEISTPLVSQKAAYADSTSGVALASEDKEDGSEAPAAAGIFAVEQKSFIPAAAGAGAVATTVAAVGAVEALPEEKDSSLKGNAFASSLDPIQGKSQKLFERDVSEEEPSTAGATKDTIDTFEAIEAPQQKGVAPIAVAEVDTVQDHSKKMPSNEFGTQPIDENAKPSKVVQEETIVAAPVTGLDESHPDTAEHVHAEKDFTETPIAEPKTGSGASQKSKVTSPANDYDIVVVEGELGKLPAKSSAADASAAISEAPASHEKPADVVEGVPKESELVPKESADAFESNVSSSEQKVNVVEEIGVITSPEQEAELIRKTLDPKASSPGETEELLIVEGDIPPSQIAEYQEASEAAAAKAMANQNPEEAVVREEPSTNAAPKEPVKETSNVAHAKKASPVKQKPAASSTSKPAEKEKKKKKGLFSKIKKIFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>C5DD10</accession>
    <name>C5DD10_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">chitin synthase</fullName>
            <ecNumber evidence="2">2.4.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0B07392g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.4.1.16" evidence="2"/>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552109.1">
        <property type="nucleotide sequence ID" value="XM_002552063.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DD10"/>
    <dbReference type="FunCoup" id="C5DD10">
        <property type="interactions" value="117"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DD10"/>
    <dbReference type="GeneID" id="8290949"/>
    <dbReference type="KEGG" id="lth:KLTH0B07392g"/>
    <dbReference type="eggNOG" id="KOG2571">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004760_3_1_1"/>
    <dbReference type="InParanoid" id="C5DD10"/>
    <dbReference type="OMA" id="AWILHYV"/>
    <dbReference type="OrthoDB" id="26569at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030428">
        <property type="term" value="C:cell septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004100">
        <property type="term" value="F:chitin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006031">
        <property type="term" value="P:chitin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04190">
        <property type="entry name" value="Chitin_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004835">
        <property type="entry name" value="Chitin_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013616">
        <property type="entry name" value="Chitin_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914">
        <property type="entry name" value="CHITIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914:SF9">
        <property type="entry name" value="CHITIN SYNTHASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01644">
        <property type="entry name" value="Chitin_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08407">
        <property type="entry name" value="Chitin_synth_1N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="722"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="758"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="792"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="842"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="873"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="963"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1018"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin synthase N-terminal" evidence="12">
        <location>
            <begin position="302"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="48"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="62"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF08407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR21671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1054" mass="120619" checksum="CBF8FACB4126F55B" modified="2009-07-28" version="1">MSYRYNHGYGNDQTNNGENPFMSSRGERSDQSYEEDQREQEYKDFVKQYPTTYQAQQTTPYNTSSGVFQGNSQQNSEPYPPHDGHQLPQSYNVPTINVYQAPQTIPFAPNTVAPMTSMNHETSNDQIYHMDYEPRNSSANSSTGAEYYPQETQPILDHRRPPPAPQPDFLSSNLNSSSVFYDYDPEDPVGFVDQNGDDYQINTFYNNENEMANPYSSAFNLGDYADNVDSGDEYSNTNYDDRQERDLGEYSYDNDANTLAKIRSSTNLLEDGASGDVLMNSAEKLGDNSSASSHNRPSRRLTTVRKFKLSMGNFIFDCPISEHLISQYVRAVDDPEKLSNEFKFMRYQAVTCEPEMFQSNNFTLRQLKYGLPRQTELMIVITMYNEDDVLLARTLKGVMDNIRHFTKKTNSSTWGPDAWKKIVVCIVSDGRSKINERSLALMSALGCYQDGFAKGEINDKKVIAHVYEHTSKVNIRGIRDGKVDLLCGENTVPIQLLFCLKENNQKKINSHRWALEAFAEVLQPTVINLLDAGTMPGKDSIYELWREFKDPQVGGACGEIKTDLGRGYKKLLNPLVASQNFEYKLSNILDKTTESNFGFISVLPGAFSAYRFEAIRGEPLRKYFLGDDLNARLFMTNMYLAEDRILCFEVVTKKDSNWTLKYSRSSYGSTDVPEKIPEFILQRRRWMNGAFFAALYSFLHFYKIWSSGHSFIRKLMLNLQFVYLFLSTLISWFSLSSFFLVFRILTMSIAVSNNDQKVFRVLSVLFLWLYGVSILITFILSLGNKPKGTSKFYLSTFIFFAILMVYMIFCSIFMSVHSIRNIIDNGNVTFVSLMKQETFRDLIVSMGSTYCLYLLSSVIYLQPMHMLTSFVQYLLLSPSYINVLNIYAFCNVHDISWGTKGFVAKPLGKIHSKEDGTVKLEIPVSAREIDANFNKHREILMSEAPEEGQEEVSFEEKKTNYYAMVRSLVIILWVISNFIIVAVVLETGGIGQYESLDKMQSSTSTETTVSILPQRSTVYFSVILWLVAFMAAFRFIGCCLYVVQRFFRKLRFGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>C5DD89</accession>
    <name>C5DD89_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0B09240p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0B09240g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552188.1">
        <property type="nucleotide sequence ID" value="XM_002552142.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DD89"/>
    <dbReference type="FunCoup" id="C5DD89">
        <property type="interactions" value="75"/>
    </dbReference>
    <dbReference type="GeneID" id="8291031"/>
    <dbReference type="KEGG" id="lth:KLTH0B09240g"/>
    <dbReference type="eggNOG" id="ENOG502RXHE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103810_0_0_1"/>
    <dbReference type="InParanoid" id="C5DD89"/>
    <dbReference type="OMA" id="HEDFPYY"/>
    <dbReference type="OrthoDB" id="5330253at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018809">
        <property type="entry name" value="DUF2406"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28186">
        <property type="entry name" value="MEIOTICALLY UP-REGULATED GENE 9 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28186:SF1">
        <property type="entry name" value="MEIOTICALLY UP-REGULATED GENE 9 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10295">
        <property type="entry name" value="DUF2406"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="171"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="180"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="197" mass="21736" checksum="E59868A30716ED0E" modified="2009-07-28" version="1">MGLFRKSSNASTGAGHLHAPDTKLTKEDREHFKLRASSVHDPVLEAVQEAQPFEQAADVSQDDPNRKSYYSGDGPDHLTRDVFGQPIQQPDISNPMRARDERPLDTIRGFEYSISGDPSWGQRLETHQYGFRVRPDFPQFGNSGDAMAASGPGGMPSSQFMMQEQAIYSAPAPGSALNPSKKKKKRGLFGRKKDTAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDC1</accession>
    <name>C5DDC1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Mitochondrial escape protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0B09966g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10 11">Plays a role in maintaining the mitochondrial genome and in controlling the mtDNA escape. Involved in the regulation of mtDNA nucleotide structure and number. May have a dispensable role in early maturation of pre-rRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Mitochondrion inner membrane</location>
            <topology evidence="1 11">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the YME2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552220.1">
        <property type="nucleotide sequence ID" value="XM_002552174.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDC1"/>
    <dbReference type="FunCoup" id="C5DDC1">
        <property type="interactions" value="171"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDC1"/>
    <dbReference type="GeneID" id="8291064"/>
    <dbReference type="KEGG" id="lth:KLTH0B09966g"/>
    <dbReference type="eggNOG" id="ENOG502QS0P">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007861_1_0_1"/>
    <dbReference type="InParanoid" id="C5DDC1"/>
    <dbReference type="OMA" id="FQFFRPY"/>
    <dbReference type="OrthoDB" id="10267654at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12433">
        <property type="entry name" value="RRM_Yme2p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018850">
        <property type="entry name" value="Mt_escape_2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039627">
        <property type="entry name" value="Yme2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034260">
        <property type="entry name" value="Yme2_RRM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32198">
        <property type="entry name" value="MITOCHONDRIAL ESCAPE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32198:SF2">
        <property type="entry name" value="MITOCHONDRIAL ESCAPE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10443">
        <property type="entry name" value="RNA12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 11" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="193"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="Mitochondrial escape protein 2 C-terminal" evidence="13">
        <location>
            <begin position="347"/>
            <end position="770"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF10443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR21782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="807" mass="92073" checksum="70679943DB2FFB75" modified="2009-07-28" version="1">MLALLSRSLRPVRYGRVKPNILLPLKRQVSTEIQEKDEQAGESTTATDTGVIHKTEEETLIYFDNVYPRATSLWSPTQWYNILLTNQSRDAVRSKIMEFASPPRNPIHGLELRSTIPVKRDGGVFATFLVPRDYSKAQVNSIIQQNTAKESSKSILSFVTRAAAFPVKGSPWIEDLRRLPSTVIKIKFEGQPLTEEEIYSLFRRYGTILDIYPSKKGDTVATLRYRSYRGAICAKNCVSGMEVHNSTIHVQYQRLADEHMIRDFFVNHQRIAIPLMIALLSIVAVLVFDPIREFSVRQKITHRFAFSRENYLIKELFKLTSFTVSSFKSLIGSENVETNKKQLWEERIEKVSDLKMWLEENNNTFVIVRGPRGSGKHDLVIQHTLHDRPNVLYLDCDKLIKSRTDSKFLMNAAAEIGYFPIFPWINSFTNVVDLAVQGLTGQKTGLSESKETQFRNMLTTALMAIRQVALKGYHPIVGRGDDAITMKEEDYLQQHPEKKPVIVIDRFNDKAEMNGFAYKELSDWAAMLVQMNIAHVIFLTETVSPNQLLADSLPNQVFKTLILSDASKESARSYVLASLDHTEPLDDDQEKSKPIILSDDIKRELDESLEPLGGRMLDLQAFVRRVKSGENPKEALDKMVEQALEQITQIFLAENVDQVKSAQAWELIEMLSKEPVVEFKNVVFRPLFKSAPEAGLLELEKNGLITVSRDRGVLKDIRPAKPLYRAAFGYLLNDSELFTVLETGYLLRIINFETKRIQKWEEELKSLGKVSEHRMFKSRLLYLAAKIDASSDSINKCEDKIKALARS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDC4</accession>
    <name>C5DDC4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0B10076p</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="9">KLTH0E00440p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0B10076g</name>
        <name evidence="9" type="ordered locus">KLTH0E00440g</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
            <strain evidence="8">CBS 6340</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2009-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Genoscope - CEA"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CBS 6340</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552223.1">
        <property type="nucleotide sequence ID" value="XM_002552177.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553508.1">
        <property type="nucleotide sequence ID" value="XM_002553462.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDC4"/>
    <dbReference type="GeneID" id="8291069"/>
    <dbReference type="GeneID" id="8291644"/>
    <dbReference type="KEGG" id="lth:KLTH0B10076g"/>
    <dbReference type="KEGG" id="lth:KLTH0E00440g"/>
    <dbReference type="eggNOG" id="ENOG502RZ2S">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028136_0_0_1"/>
    <dbReference type="InParanoid" id="C5DDC4"/>
    <dbReference type="OrthoDB" id="3364175at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051127">
        <property type="entry name" value="Fungal_SecMet_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47424:SF6">
        <property type="entry name" value="PROLINE UTILIZATION TRANS-ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47424">
        <property type="entry name" value="REGULATORY PROTEIN GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="7">
        <location>
            <begin position="9"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="49"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR21785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR23071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="688" mass="77704" checksum="AEB7A13C6C658379" modified="2009-07-28" version="1">MPRKKMMKACIKCKQRKKKCSGQLPCDYCVKIEQPGGCEYRTRTKSKVVKVTERYISSLKNKIRALEAELAKASEGPDSEGCLEDGATSAEVNPLIEPEFRLTPDLDSPKQESLTIGSVEHCVQASQHSKQFLGMSSCVQYLAKLKQSLVNSCGSSTEHFVATDGGWPPNDLDINRSFVENVATKELPDLRKAKELIEIAHEIIGADYMFLETGYFEDTAQHLICKNPPPLQSEAIIEYTTELLRLVAHLALGSLFDKENAESRSLGLKYHRTTLLLYSELVKTYDCAASTSLILSLLYMAYFSLSLNKATSAFVMTGSAIRTMFTLGLHKRTKTIVENRVFWLCFIYDRLLAVRFGFPLMIDERNINIPLLTEVDDNLTAVSLDIYHFVSQVRLAKITTQIITKIYTRNPYSFLHNCHAVLKQLKNWFESLPAELKFDYNDIKTATTRSTFNLHINYNYSIIIATRPVLLYVFNSILTENRTKEKIYEARQLELISVLLESCVQAAEIQSRILAKLYYDGKLANRSFLDCHYIFSATTVLILTGYCSMLSGESIAFSGDIEGLFDFIQHNLKILQGLSEYNLAAKNFNKQLTELIDMMSSDEVVKALATGRSQNDPTSVDFQQAVASPRPMSRILRKRGNNPPKDFEEIGYVDLSQLVNNMDAEFRSGSTTDLFLDEDFMNYASGSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDG3</accession>
    <name>C5DDG3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA helicase</fullName>
            <ecNumber evidence="4">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0C00770g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DNA2/NAM7 helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="4"/>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552262.1">
        <property type="nucleotide sequence ID" value="XM_002552216.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDG3"/>
    <dbReference type="FunCoup" id="C5DDG3">
        <property type="interactions" value="578"/>
    </dbReference>
    <dbReference type="GeneID" id="8291120"/>
    <dbReference type="KEGG" id="lth:KLTH0C00770g"/>
    <dbReference type="eggNOG" id="KOG1803">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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        <property type="component" value="Chromosome C"/>
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        <property type="term" value="F:5'-3' DNA helicase activity"/>
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        <property type="term" value="F:ATP binding"/>
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        <property type="term" value="F:hydrolase activity"/>
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        <property type="entry name" value="DEXXQc_SMUBP2"/>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolase"/>
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        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
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        <property type="entry name" value="Helicase_ATP-bd"/>
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        <property type="entry name" value="P-loop_NTPase"/>
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        <property type="entry name" value="SF1_C_Upf1"/>
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        <property type="entry name" value="SMUBP-2/Hcs1-like"/>
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        <property type="entry name" value="SMUBP-2_HCS1_1B"/>
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    <dbReference type="NCBIfam" id="TIGR00376">
        <property type="entry name" value="IGHMBP2 family helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43788:SF8">
        <property type="entry name" value="DNA-BINDING PROTEIN SMUBP-2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY MEMBER"/>
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        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="SMUBP-2_HCS1_1B"/>
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        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0347">Helicase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="185"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="12">
        <location>
            <begin position="202"/>
            <end position="414"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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            <dbReference type="ARBA" id="ARBA00022490"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048432"/>
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    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00487"/>
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    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR21824.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="659" mass="74258" checksum="41EDE3FEA233B274" modified="2009-07-28" version="1">MNKVIGERFLECIAHEQSQDTELTARLLETLPLSQLVSKGLAINHLTLQNVRSGLAGRLYVELGPDTAIDQEIIRGDVRNGDLVVIRPSGAKKDAFSCEGVVTKCSEKCITVAVDEAQEKDALNLYSSLRLYLLKTTNTITYKRMQSTLRKLCEFDGVPQNEVIQYLLDAPVFTSQTQPLKVDFFNPDLNTSQKKAIEFSLQNRISIIHGPPGTGKTHTVVELVHQLYNKGQRVLVCGPSNIAVDTILERLAKVMPGESLLRIGHPARLLESNLSHSLEVLSKSGDAGAITKDISKEIDDTIASIKKLKSYKQRKEQWKEVRELRKELKLREKKVISDLVLSARVVVSTLHGSSARELVDCYKHTDHIFDTLIIDEVSQSLEPQCWIPLIAHYKSDIQKLVLAGDDKQLPPTIKTEDNTKVKNTLGTTLFDRLVKKYGDSFRNLLDTQYRMNSEIMEFSSIKMYDGKLKAADSVASWTLVDLPATECNENTEVPLIWYDTQAGDFPERAEDQDSGSLVSSKYNENEAFLALHHVTSLIDSNVPQECIGIISPYNAQVAFLKKTVHPQYPLVEISTVDGFQGREKEVIILSLVRSNDKFEVGFLSDQRRLNVAMTRPKKQLCVIGNMETLERSGIEYLKDWVRWSEEHSEIRYPDLSEIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDG8</accession>
    <name>C5DDG8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0C00880p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0C00880g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552267.1">
        <property type="nucleotide sequence ID" value="XM_002552221.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDG8"/>
    <dbReference type="FunCoup" id="C5DDG8">
        <property type="interactions" value="393"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDG8"/>
    <dbReference type="GeneID" id="8291125"/>
    <dbReference type="KEGG" id="lth:KLTH0C00880g"/>
    <dbReference type="eggNOG" id="ENOG502QQXX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006632_0_0_1"/>
    <dbReference type="InParanoid" id="C5DDG8"/>
    <dbReference type="OMA" id="DCQDWRL"/>
    <dbReference type="OrthoDB" id="2264294at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001080">
        <property type="term" value="P:nitrogen catabolite activation of transcription from RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050797">
        <property type="entry name" value="Carb_Metab_Trans_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31668">
        <property type="entry name" value="GLUCOSE TRANSPORT TRANSCRIPTION REGULATOR RGT1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31668:SF4">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN DAL81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="8">
        <location>
            <begin position="134"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="54"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="221"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="806"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="898"/>
            <end position="1085"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="95"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="227"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="806"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="825"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="898"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="910"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="942"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="976"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1014"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1056"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1073"/>
            <end position="1085"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR21829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1085" mass="121647" checksum="62F478675B0301A7" modified="2009-07-28" version="1">MTNNTLDNWNSGASGGQGSGNVNNSMLNLNDDYTNILNNLGNSQSQLLGDDLANRHDSQSSQQSPYNLPNLNARPSNEKQPNLDLLLQHYQELFSRSSGGSGDNSPDPSGVVAPNTNSKALAAQENKSVIAEKPCDHCRRRQIKCVIAPDLPNCVQCENKGIKCAYSELPSSRVPTDIVPDKQKRSRVDENVDVHELLKRAKLNNNDSVSQYYSDLLQNLNQSSNSRNPSGPSQYPTRSNQLNPFMMYGTPGLNDQASKSGTASVHNGASPAYLSGNTTYNTNNPIAQKMAYTPPPIQYPRSSFYVGPTSVFDINLVNHVKLDKIDQVQLSKTLALRKVAPNVQFLLRDDLNQQLYMKQEQEIDMVEKLVHPHGKILVDIFFKLIHPQYPILHERVFLEKYSRSYRELTAPLLAALYSLSLQWWDFHPQLIGFPKPDVVNQLNEIALRTFFDVVERPKLSIIQTGLLILQCRSESSSNWVICSEIVALAEDLGLGIDCQDWRLPGWERGLRRRLAWAVWLEDKWASMMEARYSHLILGRNWLVKMLADEDFPESSPVINDSNGNNKMNYQGLKDVTSPVGNISMLDMTPTGEDFKNGKLLFQHMVSLSIILGEVMDTFYTLGALKTVTKIEQVLKLAKPLQLKMREWYHSLPSQLSMNNFQQRRFTSNASLTLSYFAVELTLHRKIITTLNSETSPQLVQVCRLAAKTRLIAAIEFVRDLKFEHVNSFWYTCSTGNLTLIGTFAALLYVTAPSKEESIVFRDCLRNFVWILRMSSKGFEKAENGLKKIHMLLAQIPGLLTDDPVVPQQPQQSFVAPPSESPMMPQQQLQQQQQRQQQQQQQQQQQQQQQQQQQQQQQAGQQSYGAALSQSPFDPQRSFNQLRQLPPEILQQLASLQNNMPSINSSSSTEETMKPSDHRSVKPSRPEGPENRETLPDFHNMSPELTSLDLRSSPQKNTRAQPEHESSTRPEQAVGSEDTQQSPTKTPGITEKSKILTPQSVYGANGKKANASEQNDDHSREINNYDPKREGSHVSPASLAESNGNDKPVDSGNAQKVSRDLEDSATAESKETKSGSGTSSPSSDQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDQ2</accession>
    <name>C5DDQ2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0C02838p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0C02838g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552351.1">
        <property type="nucleotide sequence ID" value="XM_002552305.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDQ2"/>
    <dbReference type="FunCoup" id="C5DDQ2">
        <property type="interactions" value="400"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDQ2"/>
    <dbReference type="GeneID" id="8291212"/>
    <dbReference type="KEGG" id="lth:KLTH0C02838g"/>
    <dbReference type="eggNOG" id="KOG2741">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023194_25_0_1"/>
    <dbReference type="InParanoid" id="C5DDQ2"/>
    <dbReference type="OMA" id="KVPHHRE"/>
    <dbReference type="OrthoDB" id="64915at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055080">
        <property type="entry name" value="Gal80p-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051317">
        <property type="entry name" value="Gfo/Idh/MocA_oxidoreduct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43708">
        <property type="entry name" value="CONSERVED EXPRESSED OXIDOREDUCTASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43708:SF1">
        <property type="entry name" value="GALACTOSE_LACTOSE METABOLISM REGULATORY PROTEIN GAL80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22685">
        <property type="entry name" value="Gal80p_C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase N-terminal" evidence="1">
        <location>
            <begin position="18"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Gal80p-like C-terminal" evidence="2">
        <location>
            <begin position="156"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR21913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="424" mass="46880" checksum="53A54D6CDA7E965A" modified="2009-07-28" version="1">MNNNKRSYVSTVPKANPLRVGFIGLSAYKGWAIKSHYPAILQLPSQFQVTALLNLDIETSLATIKKLKLTKATAFSDVSSFASYDNVDMVVISVNVPELFTIVMELLENSKANPNLKYIYSEWSLGKNLKEAEAIHKAAKERGVQTIISLQGRKSPYVVRAKELVSEGCIGDINSIEIAANGGWYGYERPIKSPKYLYDAASGVSLLSIAFGHTIDILQYITASYFSEINAMIFNNIPYQVLLDEHGKKTGEKAEKTAPDHLLFQGSLSHGNVPVSCSFKGGTPVKKFSKNLVIDIHGTKGDLKLEGDAGFAEMSNLVLYFCGLKKEGSMNVSTGSSGAETMEVYHLRNYNSVVGNILRIYEAIADFHFRTNPRTKLPPKLERQGFLFEGFPTFKDALIVHQLIQRVIESSQQKTTLPTSFEAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-04-02" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>C5DE44</accession>
    <name>C5DE44_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0C06160p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0C06160g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552493.1">
        <property type="nucleotide sequence ID" value="XM_002552447.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DE44"/>
    <dbReference type="GeneID" id="8291361"/>
    <dbReference type="KEGG" id="lth:KLTH0C06160g"/>
    <dbReference type="eggNOG" id="ENOG502S9RK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_746112_0_0_1"/>
    <dbReference type="InParanoid" id="C5DE44"/>
    <dbReference type="OMA" id="PCKINEA"/>
    <dbReference type="OrthoDB" id="4068074at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="184"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="274"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="200"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="274"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="371" mass="41144" checksum="D28556CE7AE1CFDF" modified="2009-07-28" version="1">MANSLLSLYSDSWTLSERPGSNLIKFDAPASDSGSSVILDRSNAVEGSIDSVSPQQLRRFTADVDIQRVGDLSVLSMAQRSLETLRRVEESMHTDNVFLTEVMVMHRLAWDIDVYSQTKSEFPLENVPIKCNILKTLFELLRDYQESKPELPLENVAKVLHYLNKLIQASQLEVPAPQSLAPQPLAKVSSATQPLRKTRTGVSSRSSHVGGHDSYSSNIVRETPVYNSRVSMHSGSGKSRIFSKFFTPARSRESMHTRSTITVQPVNNMAATANTSSTATTNSGSLTSGGSDSEPLAPDPVLISHKLQDIESMKNYKESIVALAKTLRSLPQTDQNTIIFHFVDKSIVPFILKDCKLLLMHYLHEEVILKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>C5DE60</accession>
    <name>C5DE60_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0C06512p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0C06512g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552509.1">
        <property type="nucleotide sequence ID" value="XM_002552463.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DE60"/>
    <dbReference type="FunCoup" id="C5DE60">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="GeneID" id="8291377"/>
    <dbReference type="KEGG" id="lth:KLTH0C06512g"/>
    <dbReference type="eggNOG" id="ENOG502S9AV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1461559_0_0_1"/>
    <dbReference type="InParanoid" id="C5DE60"/>
    <dbReference type="OMA" id="RIREPYS"/>
    <dbReference type="OrthoDB" id="4036589at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="148"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="16"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="28"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="80"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR22071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="185" mass="21557" checksum="C8EEB341E165E605" modified="2009-07-28" version="1">MQPFSSPPRVEHRSYYDQSPDSPPSYDTKLSTPTSGLDASVVDKLTSERRRNYQRVKQPFSSPLKAEKHSGSRPSPQRRQQNEQRHRERESRAMKARGGAFKMEKDVMDLEKKCELRLLEREAEFYQLDPDNVAALESEQEALEDEELVALLQEREEYEKLLELEQQQIEEALACFTLSNSEEHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>C5DE95</accession>
    <name>C5DE95_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="14">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">KLTH0C07326g</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="14"/>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552544.1">
        <property type="nucleotide sequence ID" value="XM_002552498.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DE95"/>
    <dbReference type="FunCoup" id="C5DE95">
        <property type="interactions" value="1022"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DE95"/>
    <dbReference type="MEROPS" id="C19.083"/>
    <dbReference type="GeneID" id="8291413"/>
    <dbReference type="KEGG" id="lth:KLTH0C07326g"/>
    <dbReference type="eggNOG" id="KOG0944">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009884_1_0_1"/>
    <dbReference type="InParanoid" id="C5DE95"/>
    <dbReference type="OMA" id="FVPCEHT"/>
    <dbReference type="OrthoDB" id="361536at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02658">
        <property type="entry name" value="Peptidase_C19B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14296">
        <property type="entry name" value="UBA1_scUBP14_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14298">
        <property type="entry name" value="UBA2_scUBP14_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000086">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033864">
        <property type="entry name" value="UBA2_scUBP14-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016652">
        <property type="entry name" value="Ubiquitinyl_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041432">
        <property type="entry name" value="UBP13_Znf-UBP_var"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF664">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17807">
        <property type="entry name" value="zf-UBP_var"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016308">
        <property type="entry name" value="UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00290">
        <property type="entry name" value="ZnF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50271">
        <property type="entry name" value="ZF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 14" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 14" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="7 14" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBP-type" evidence="17">
        <location>
            <begin position="156"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="16">
        <location>
            <begin position="309"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="15">
        <location>
            <begin position="564"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="15">
        <location>
            <begin position="628"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="11">
        <location>
            <position position="318"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="715"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR016308-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR016308-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAR22106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="762" mass="85704" checksum="E38272EA22014B78" modified="2009-07-28" version="1">MSELEEFGLLQIPSVIHKDDCGYCFESMYNDDQMASHALNVCLTCFQSFCDTHIPVHQSVAKNDFNDKHDFLLKVSKIRKPEQKQPIEKKLKLEITEKSEDDTYESLWWLTKGAVIIADYRTQGLNSSVTTKVTEILNARSSNFQNMTQAWQLEVKPCVHVENLEVPNDNPQTVANRCTDCKLGSNLWMCLHCGNVGCGRQQVGIEGHSHALKHFESNPGHPLAVKLGSLSNSAADIYCYSCDDEVQFADTKKWIQLLLYWGIDIQSRVAQEKTLLELQVEQNMNWDFQMVDAEGHELRHLEGSKKYGYGLQNLGNSCYLNSVLQVLLNGDLSRWSLEGLGDFPGDVLYVSSNLRCQLIKMRNALKNEPEKYPHGVKPTTFKHCIGGTHEEFSSGRQQDALEFFSYFVDLLDKKVFEKSSSNPNDLMKFSLQDRIECTKCHGVKYTSQVSDYLQVLLSDSEEPQDLLANLAQNFQGDEVEFKCPTCNETTKANKSCGFRTFPEILVASCSRIKLVNWVPVKTSQEVLVPGTQAADTAILELGQFKSNGFDPDNETLLEKGEPAGFEPKANCIIQLTEMGFSENAAVRALYHSGNSSAEVAANWLFQHMDDADLNDPFIVPSAPSKAAEVNPQALENMAAMGLNPELCRKALIINQGNVSASVEWVFNNLDYCDSSIQESTVDEDDKEYGITDKDKANYRLKAVVCHKGASVHSGHYVAFIKKQVENEVKWVLYNDEKIVVADSPTNIEEIKKNGYIYFFDRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEF3</accession>
    <name>C5DEF3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0C08712p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0C08712g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552602.1">
        <property type="nucleotide sequence ID" value="XM_002552556.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEF3"/>
    <dbReference type="FunCoup" id="C5DEF3">
        <property type="interactions" value="353"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEF3"/>
    <dbReference type="GeneID" id="8291476"/>
    <dbReference type="KEGG" id="lth:KLTH0C08712g"/>
    <dbReference type="eggNOG" id="ENOG502S1F7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115506_0_0_1"/>
    <dbReference type="InParanoid" id="C5DEF3"/>
    <dbReference type="OMA" id="QNQVDTQ"/>
    <dbReference type="OrthoDB" id="690068at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11387">
        <property type="entry name" value="bHLHzip_USF_MITF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BHLH" evidence="2">
        <location>
            <begin position="16"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="163"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR22164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="183" mass="19886" checksum="09DA4C53491BF16E" modified="2009-07-28" version="1">MSSIPRSNSVSSSVPSTAGTTMENDKKRRGNINDKIQQLLSMIPPEYFQDYYRRSDSIDYENSQMTPGGSILSSAGMSSKNKGTGTKDGKPNKGQILTQAVEYISKLQNQVDARNREEVELILRVKELSKKTGVAVDDINLENTSAEVALAKIGVGPLAGVSEPYYGSGNQKNNPADYEGCEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEG4</accession>
    <name>C5DEG4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0C08954p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0C08954g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552613.1">
        <property type="nucleotide sequence ID" value="XM_002552567.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEG4"/>
    <dbReference type="GeneID" id="8291487"/>
    <dbReference type="KEGG" id="lth:KLTH0C08954g"/>
    <dbReference type="HOGENOM" id="CLU_2097304_0_0_1"/>
    <dbReference type="InParanoid" id="C5DEG4"/>
    <dbReference type="OrthoDB" id="4031750at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR22175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="120" mass="13759" checksum="6165C5DF1317A38A" modified="2009-07-28" version="1">MSSVSFTKKELLTCIKASSTWAQWDNPNEGLVDLIQPFVDGPYEVKDFSAANSEAAQEARDRVNHLIGKHILKTKLSSGSWYAYTKGKKQYRFALRYCSEPTTLYFKRITRSLSREKQLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>C5DES4</accession>
    <name>C5DES4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0C11682p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0C11682g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552723.1">
        <property type="nucleotide sequence ID" value="XM_002552677.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DES4"/>
    <dbReference type="FunCoup" id="C5DES4">
        <property type="interactions" value="77"/>
    </dbReference>
    <dbReference type="GeneID" id="8291610"/>
    <dbReference type="KEGG" id="lth:KLTH0C11682g"/>
    <dbReference type="HOGENOM" id="CLU_1337710_0_0_1"/>
    <dbReference type="InParanoid" id="C5DES4"/>
    <dbReference type="OrthoDB" id="4036555at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="153"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="205" mass="22714" checksum="A919700231EDE1EB" modified="2009-07-28" version="1">MNVRSPGKLRRALSGKNINSKSGLRKRSASLSPKKLGTARRSAEHSVLQVKSIEKVQPFQFFEQSVEDQQAVSSAQNKLCHAGDDKVCTTKEKENIPLDNPNCRFGSKPTQVSNERAPLSRLNADDYGGVLQIHDLPTYADSEHQSENLIEVSGFTTPPRGGKTGTDTAFHSFNTSPRKIETQQGREERTSFSALKVARKLKFED</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEV2</accession>
    <name>C5DEV2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0D10010p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0D10010g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the importin beta family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553145.1">
        <property type="nucleotide sequence ID" value="XM_002553099.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEV2"/>
    <dbReference type="FunCoup" id="C5DEV2">
        <property type="interactions" value="1126"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEV2"/>
    <dbReference type="GeneID" id="8295384"/>
    <dbReference type="KEGG" id="lth:KLTH0D10010g"/>
    <dbReference type="eggNOG" id="KOG1993">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003886_0_0_1"/>
    <dbReference type="InParanoid" id="C5DEV2"/>
    <dbReference type="OMA" id="SFHYVFH"/>
    <dbReference type="OrthoDB" id="361693at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058669">
        <property type="entry name" value="TPR_IPO7/11-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997:SF7">
        <property type="entry name" value="IMPORTIN-11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997">
        <property type="entry name" value="IMPORTIN-7, 8, 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25758">
        <property type="entry name" value="TPR_IPO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="5">
        <location>
            <begin position="34"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR22707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1034" mass="118930" checksum="0B33D14F2F3CCF01" modified="2009-07-28" version="1">MGDGSLALTELDLVQVLGQASDPRHAGSQAQRFAEQQLKLWETQKGFHFLLQSVYLNLSCPLDVRWLAIIQFKNGVEKYWRSTRINAIGKDEKASIRARLFDLIDENNNQLGIQNSQAAARIARLDFPGDWPTLFEDLEHLLGTEAIWQDNVRMFNLLLIMNQMIKTLATARIGRCRPAMQSKSPLVFPLLVRTYLKAFNEWTSAASVDEESLSQIQVSYLALKVLRRLVTEGCETPHRNVSIAEFLQISINHFELLMTHFDNYKGFDNFEKFVRCYGKLYYSLICASPSNFILLPCSLQVLTAFTKLLIERAPDVYRENAEVDGNFWEQIAIRSFLILKRLINFIHKKGAVTIKAKNDKYEVDVAVKRITSEFLNENLIKKWVDLLMDWYLKLRKSDLEDWSLDPEEWINEQLATSYEYQIRPCAENFFQDLINSFPDLLVPYLLNKIQTEASSLNNSVEDLLKKDAIFASFQLSATSISEAVDFDTLLVNVFLPEALNPQQLPEFSKIVKRRVALVINEWSTIKCSEESKQACYKFFYKILTEEEDKVVRLTAIQSLRTLVDDWNFDKNSFQPYLDDFVVVLLKRTLPSVSLTETRLYVLNTLSDIIVQTKPLVSRNLLVEILQVVPELWELSMNDSSESILANALLRLLRRAIDSLGKNSPATWQISLPITLAACDPSSPQYSLLYEDGFELWQSLLQNYSPSEQALDETFFKLVPFLKNAVQNQTEILPTLLEIVKSYALVLPESQFLNNECFFFAFSHLSGYLLKLRDDSFDIVLSILDILTLARDPDNDRLLLEFLLETGMLPSIFNALFREDQISLFQAGQLLQTIARIAYFDPGSIIQLLERYRSALPTSERNSLLPLEERKALSVETPLENVINKLVTIWILCFKDFFDPRVRKIHILGLSSMLRTQYPAVISEIASIVSLWVEFLEETNETTNGDCEKYYINDPAYGESQDDSEEHILTCEHQRLQKLVSERDPAHNVSLKEHITYTLQYLETEMGPQFQTLLSSVDEALIQDLRLFLSIPAQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEW5</accession>
    <name>C5DEW5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0D10318p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0D10318g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S9B family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553158.1">
        <property type="nucleotide sequence ID" value="XM_002553112.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEW5"/>
    <dbReference type="FunCoup" id="C5DEW5">
        <property type="interactions" value="335"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEW5"/>
    <dbReference type="ESTHER" id="lactc-c5dew5">
        <property type="family name" value="DPP4N_Peptidase_S9"/>
    </dbReference>
    <dbReference type="MEROPS" id="S09.005"/>
    <dbReference type="GeneID" id="8295397"/>
    <dbReference type="KEGG" id="lth:KLTH0D10318g"/>
    <dbReference type="eggNOG" id="KOG2100">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006105_0_1_1"/>
    <dbReference type="InParanoid" id="C5DEW5"/>
    <dbReference type="OMA" id="NYDMHIF"/>
    <dbReference type="OrthoDB" id="16520at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000003">
        <property type="entry name" value="Dipeptidyl peptidase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.140.10.30">
        <property type="entry name" value="Dipeptidylpeptidase IV, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002469">
        <property type="entry name" value="Peptidase_S9B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050278">
        <property type="entry name" value="Serine_Prot_S9B/DPPIV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731:SF160">
        <property type="entry name" value="DIPEPTIDYL AMINOPEPTIDASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731">
        <property type="entry name" value="PROTEASE FAMILY S9B,C DIPEPTIDYL-PEPTIDASE IV-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00930">
        <property type="entry name" value="DPPIV_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0720">Serine protease</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="Dipeptidylpeptidase IV N-terminal" evidence="9">
        <location>
            <begin position="199"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="8">
        <location>
            <begin position="658"/>
            <end position="863"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR22720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="871" mass="99536" checksum="7E52FD45E85736DE" modified="2009-07-28" version="1">MEPSYKKRHPSLYLLKSNSSAQFMPAQEFELQPKGEAPGTAGKLPRDEEAQVTDGADFLSRVPFRDFQNRIRVRKSAVIFLALIISWLTVGFSVLRFKDSNLSLSKPASPSGRTFSIENVLNGEFSYYDTYFKFIRPGVLHQPQDNDPGLYLKIENKNGIPKYVAKQLVDVEFYKELGRTSFTYGDVEYQVSDFDVSHRLDKAILATDLEKKFRHSSTAYYWLKDIPTNTYRPISPFPEDGKLYKLSFARFSPHRNFVYFVYNNDLYVQSATNSQGFRRLTADGSEDVKNGRPDWVYEEEVLASDQAVWWAPDDSQIVFAKFNDSEVLTESVPTYINEQAYDDVQQIKYPKPGTPNPRVQLFSYKMSEGVTYSIPFGGETGGRILYAAQWLDNNHFMFKDTDRASQAMFVKIYESNARKVSTVRIINATAYSGWIEKVKDVQPIPPSEIHGRSELSYVDVQVDESGYPHLFHFKSVFASTGTQLTFGDWEVTGKGIVGYDQDADSVFFLSNRLSRFSQHLFMVPLREDSTKAAHGLQDPRDRDSFYEFELSVSCRFGVKRYLGPDEPMSDAGVLTSLLLNDQSRAITQLTSNAGMLEALKKYDLPKSSFSSTQLDDDVQIDYKETVPYDFDPRKKHPLLIHVYGGPGSQTVDHKFAISLEESLASSANAIVLSIEPRGTGGRGWPYRSWAKNKIGYWEPRDIISVVKDYVNKHSDSIDPDHVAIWGWSYGGFVTLKTLELDQGQIFKYGVAVAPVTNWKYYNSIYTERYLGLFENNEKAYEDTSLIKDVQNFSSVKRFLLIHGSADDNVHVKNTMQLLDNFNLNNVKNYDLSIFPDSEHSIVFHNANRVVYERIFNWIKNAFEGKLDNLSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEX0</accession>
    <name>C5DEX0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0D10428p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0D10428g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553163.1">
        <property type="nucleotide sequence ID" value="XM_002553117.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEX0"/>
    <dbReference type="FunCoup" id="C5DEX0">
        <property type="interactions" value="488"/>
    </dbReference>
    <dbReference type="GeneID" id="8295402"/>
    <dbReference type="KEGG" id="lth:KLTH0D10428g"/>
    <dbReference type="eggNOG" id="ENOG502QQGC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010748_0_2_1"/>
    <dbReference type="InParanoid" id="C5DEX0"/>
    <dbReference type="OMA" id="TRCVKRN"/>
    <dbReference type="OrthoDB" id="65716at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056751">
        <property type="entry name" value="PAS_13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053045">
        <property type="entry name" value="Zinc_cluster_trans_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31986">
        <property type="entry name" value="REGULATOR OF DRUG SENSITIVITY 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31986:SF7">
        <property type="entry name" value="REGULATOR OF DRUG SENSITIVITY 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24990">
        <property type="entry name" value="PAS_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="8">
        <location>
            <begin position="15"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR22725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="353" mass="40592" checksum="C0FD6D5069D10434" modified="2009-07-28" version="1">MNESSQKKRKKVSKACLYCRRSHMICDEQRPCSRCLRRDIGHLCRDEDSRLPMDVEVDPERIQPTLASLDKQHPALQQPPKNVAYSGLLSQQPTFVSEHVGSEFLSLNEFMNMLEEPIILEPPAVTHSAPRNPEDQPTLKENFFLTAADPSAEMTPEDRLKSVINAKLEAGLLRPYNYAQGYARLQTYMDKHMDFLSKQRILKPLSIIRPEFRAIARSLKDVDLILVEESFERMLLSYDRVFTSMSMPACLWRRTGEIYRGNKEFASLVDCKVDDLRDGKLAIYELMTEESAVNFWEKYGSIAFDKAQKAVLTSCNLRARDGGRQRSCCFSFTIRRDRYNIPICIVGNFIPTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5DF15</accession>
    <name>C5DF15_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0D11462p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0D11462g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553208.1">
        <property type="nucleotide sequence ID" value="XM_002553162.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DF15"/>
    <dbReference type="FunCoup" id="C5DF15">
        <property type="interactions" value="287"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DF15"/>
    <dbReference type="GeneID" id="8295448"/>
    <dbReference type="KEGG" id="lth:KLTH0D11462g"/>
    <dbReference type="eggNOG" id="KOG1208">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_44_6_1"/>
    <dbReference type="InParanoid" id="C5DF15"/>
    <dbReference type="OMA" id="FTWFRYA"/>
    <dbReference type="OrthoDB" id="191139at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320">
        <property type="entry name" value="RETINOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320:SF282">
        <property type="entry name" value="WW DOMAIN-CONTAINING OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="234"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR22770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="324" mass="37012" checksum="2FF06DA46392E3A4" modified="2009-07-28" version="1">MPARNLDQLPYYDLRKQRKVALVTGGNSGIGWYTVLHLYMHGFVVYVAGRNSSRVNRAMKDIKKEARTRIKTLATDQRHPVGELHFLSLDLTALKVVEKAAKRFRSLESSLDVLINNAGVMALPYSVTEDGFEIQLQTNYVAHFLLTMRLLPSVKRCRGRIITVSSIGHNLEFSYFSLSQSFNYKPNMLFTWMRYAMAKTASIQFAKMLAIKHPDVLCISVHPGLVMKTNLFSYWTRLPIVGIFFWFFFQLVGYFFGVSNEEGSVATLKGALSTELEREWDNGKYYTTGGIESKPSYVANNLDNAASTWLWTIHELRDRGYEPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFA7</accession>
    <name>C5DFA7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0D13574p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0D13574g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553300.1">
        <property type="nucleotide sequence ID" value="XM_002553254.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFA7"/>
    <dbReference type="FunCoup" id="C5DFA7">
        <property type="interactions" value="322"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFA7"/>
    <dbReference type="GeneID" id="8295542"/>
    <dbReference type="KEGG" id="lth:KLTH0D13574g"/>
    <dbReference type="eggNOG" id="KOG4305">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001251_6_1_1"/>
    <dbReference type="InParanoid" id="C5DFA7"/>
    <dbReference type="OMA" id="FAHVNDI"/>
    <dbReference type="OrthoDB" id="2272012at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04435">
        <property type="entry name" value="DEP_fRom2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000405">
        <property type="entry name" value="RHO1 GDP-GTP exchange protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001180">
        <property type="entry name" value="CNH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000591">
        <property type="entry name" value="DEP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041675">
        <property type="entry name" value="PH_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052233">
        <property type="entry name" value="Rho-type_GEFs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46572">
        <property type="entry name" value="RHO1 GDP-GTP EXCHANGE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46572:SF2">
        <property type="entry name" value="RHO1 GDP-GTP EXCHANGE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00780">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00610">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15405">
        <property type="entry name" value="PH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00036">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00049">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50219">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="4">
        <location>
            <begin position="553"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="domain" description="CNH" evidence="5">
        <location>
            <begin position="928"/>
            <end position="1223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="71"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="434"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="71"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="87"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="122"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="163"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="195"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="208"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="229"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="436"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR22862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1244" mass="140365" checksum="3CFDC59314F8D899" modified="2009-07-28" version="1">MTEDLKGLDGRRRLFTEIFGVDRPISRLEVQGSSDHVPDRIEQIQSLHDAILTDIEEDVLPPEAEHYILSQNTEVRSVSDTQSLSSNEDRLSADSPRKIIPNSSPKRKTGQGSARPLPPPISTSFSCAANSTHVPSPQRSQSDLPRLKSSFSQLSMSGHLKKQQTNLSSTPTTLKKSPLQGFGFFSRPGSKDNFDNTQHPANNGQQANSMNSRSLSLNSSSLKNFASSFQQKASSGRTTSQKLPGSSSSIYEGSHYQVNPQNNTGLGIRSRESSASLNSSQTNINPRKLGSTPSLHYHRRSSASLHSKPILSATSMKRPQVYPAMLSRVATRFRKSVQVGEYKKDGLIYRDAFTGQQAVDVICSIIRTSDRNLALLLGRALDAQKLFHDVVYEHRLRDSPNEVYEFTGNSRIIGAGATGMVPASSQDPLLMGSHHNLMSTHPSKSTLNTSASTNSVTSEGFGKQVDPAKMHQVNGVFTLLAECYVPTCSRNKLCYSISCPRRLEQQARLNLKPNGGLKRNISMAVDDEEEEKPSWTSSVPEEVWSSLPKKEIKRQEAIYEVYITEKNFVKSLEIIRDTFMKSLAETNIIASDIRKNFLKHVFAHVNDIYSVNRRFLDALSDRQKSSPIVDGIGDIFLRFIPFFEPFVMYVASRPYAKYLIETQRSVNPYFARFDEDLMNSSLRHGIDSFLSQGVSRPGRYILLVREIIKSSDSEKHKRDLENMNKALEALREFMTRIDRASGASQDRHDIELLKQKILFKNEYVNLGLNDGKRKIKHEGVLSRKELTKSDNSFGGDIQFYLLDNMLLFLKAKAVNKWQQHKVFQRPIPLPLLFACAAEDMPPIRKYINSSPECSSGIISTEHHYSNPKNAITFSYFGAKHRYQVTLYAAQYAALQTLLEKIKQEQAALLAANDIFNMTKINSRFFDFNNKINSVASCDGGRKLLIATNQGLYVSDTKHEAASEGRKPTTYFAAPVKILHKTNITQVAVLEEFQSVLLLVDKKLYSCPVALFKEPKDGTQYFKKHAKELVNHVNFFSEGDCDGKRLVVTAHSSSHSIKYFEHDHPMLMEGKKNMKRKITEIVFESEPVSISFLRTNLCIGCKKGFQIVSVSQNVHEPLLDPADTSLEFALKDVLRPLSIYRVGASMFLLCYSEFAFFVNSQGWRKKESYVIHWEGEPQKFTIWYPYILAFDPSFIEIRKIDTGELVRCVLGEKIRLLQASSQQILYAYEDEYGYDTVMSLDFWGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFD4</accession>
    <name>C5DFD4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0D14212p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0D14212g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553327.1">
        <property type="nucleotide sequence ID" value="XM_002553281.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFD4"/>
    <dbReference type="FunCoup" id="C5DFD4">
        <property type="interactions" value="631"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFD4"/>
    <dbReference type="GeneID" id="8295571"/>
    <dbReference type="KEGG" id="lth:KLTH0D14212g"/>
    <dbReference type="eggNOG" id="KOG2785">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018787_1_1_1"/>
    <dbReference type="InParanoid" id="C5DFD4"/>
    <dbReference type="OMA" id="QRYHMKT"/>
    <dbReference type="OrthoDB" id="19329at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041661">
        <property type="entry name" value="ZN622/Rei1/Reh1_Znf-C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040025">
        <property type="entry name" value="Znf622/Rei1/Reh1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13182:SF8">
        <property type="entry name" value="CYTOPLASMIC 60S SUBUNIT BIOGENESIS FACTOR ZNF622"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13182">
        <property type="entry name" value="ZINC FINGER PROTEIN 622"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12756">
        <property type="entry name" value="zf-C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="8"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="145"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="290"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="394"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="298"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR22889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="413" mass="47348" checksum="A483C9704A122EF4" modified="2009-07-28" version="1">MSEAIFTCNSCMIQFRSSDQQRYHMKTEWHRYNLKRRISQLPPISADVFAEKLQISERAKELNQVDEFGFPILKAKTPRYKRSDEVDLSKLQRRRNRKLREDNHERSVSPTPSVASQISKLSVNSNEIHTDFDEEKSYEYGFTTDSNNEYNSSDFESTSDELSGDEASSERPSITDCIFCKAQNKEVERNLKHMFASHGLYIPERSYLIDLTGLLNFLIDTIVVANECLCCSFKGSSLQSIRAHIASKGHSRLPYETKEERRRVAGFYDFSSENEVQSNLTRNNGRSVAFEAEPDSGSEGTLPSDTDDINSNYTHAEVDDTGVELTLPSGSRAGHRSMRRIYRQNLPLPPMASDGNRTVAAGDRRFFGGVTDKTMKKNDKKSQQIERQIMNRDIRNQAKRGNFQTHYRDELLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFE1</accession>
    <name>C5DFE1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0D14366p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0D14366g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553334.1">
        <property type="nucleotide sequence ID" value="XM_002553288.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFE1"/>
    <dbReference type="FunCoup" id="C5DFE1">
        <property type="interactions" value="299"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFE1"/>
    <dbReference type="GeneID" id="8295578"/>
    <dbReference type="KEGG" id="lth:KLTH0D14366g"/>
    <dbReference type="eggNOG" id="KOG3133">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_863835_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFE1"/>
    <dbReference type="OMA" id="YEPMREM"/>
    <dbReference type="OrthoDB" id="21292at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033328">
        <property type="term" value="F:peroxisome membrane targeting sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045046">
        <property type="term" value="P:protein import into peroxisome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.900">
        <property type="entry name" value="Pex19, mPTS binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006708">
        <property type="entry name" value="Pex19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038322">
        <property type="entry name" value="Pex19_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12774">
        <property type="entry name" value="PEROXISOMAL BIOGENESIS FACTOR 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12774:SF2">
        <property type="entry name" value="PEROXISOMAL BIOGENESIS FACTOR 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04614">
        <property type="entry name" value="Pex19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="285"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="296"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="308" mass="33700" checksum="25C404342488F39E" modified="2009-07-28" version="1">MSSNIDDYDDLDEFLEDPSKLDEEQANTAAETSKSAAAMSNNNPETASAIEDLQSEFAKLMQEGGDSEENKKTVENFKQLLNVLGEAGSGDPAATTGTRQPAGATTDTGAFNGVTGASTSGKGAGFKDIVSNTLDRLKENGTKVDTNLMEEKKKGNSDDILSQLLGQLVEGDADDEEGVENAIKSMLNQMSAKEVLYQPMKEMHVEYTQWMDANSESEEHADKLPVYRQQFELVGRIVTIYERDDYEDSKYRDEVGQLLDELEQLGESPVSKGFGSADSADLNKLLEVDGNEDMSNIDKELQDTCKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFW3</accession>
    <name>C5DFW3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">L-lactate dehydrogenase</fullName>
            <ecNumber evidence="3 9">1.1.1.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0D00440g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 9">
            <text>(S)-lactate + NAD(+) = pyruvate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23444"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16651"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LDH/MDH superfamily. LDH family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.27" evidence="3 9"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552743.1">
        <property type="nucleotide sequence ID" value="XM_002552697.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFW3"/>
    <dbReference type="SMR" id="C5DFW3"/>
    <dbReference type="STRING" id="559295.C5DFW3"/>
    <dbReference type="GeneID" id="8294962"/>
    <dbReference type="KEGG" id="lth:KLTH0D00440g"/>
    <dbReference type="eggNOG" id="KOG1495">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045401_1_1_1"/>
    <dbReference type="InParanoid" id="C5DFW3"/>
    <dbReference type="OMA" id="EWDLDDY"/>
    <dbReference type="OrthoDB" id="6270329at2759"/>
    <dbReference type="UniPathway" id="UPA00554">
        <property type="reaction ID" value="UER00611"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004459">
        <property type="term" value="F:L-lactate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006089">
        <property type="term" value="P:lactate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05292">
        <property type="entry name" value="LDH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000018">
        <property type="entry name" value="Malate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.110.10">
        <property type="entry name" value="Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00488">
        <property type="entry name" value="Lactate_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001557">
        <property type="entry name" value="L-lactate/malate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011304">
        <property type="entry name" value="L-lactate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018177">
        <property type="entry name" value="L-lactate_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022383">
        <property type="entry name" value="Lactate/malate_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001236">
        <property type="entry name" value="Lactate/malate_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015955">
        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01771">
        <property type="entry name" value="L-LDH-NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000824">
        <property type="entry name" value="PRK00066.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43128">
        <property type="entry name" value="L-2-HYDROXYCARBOXYLATE DEHYDROGENASE (NAD(P)(+))"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43128:SF16">
        <property type="entry name" value="L-LACTATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02866">
        <property type="entry name" value="Ldh_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00056">
        <property type="entry name" value="Ldh_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000102">
        <property type="entry name" value="Lac_mal_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00086">
        <property type="entry name" value="LLDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56327">
        <property type="entry name" value="LDH C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00064">
        <property type="entry name" value="L_LDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8 9" id="KW-0520">NAD</keyword>
    <keyword evidence="4 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactate/malate dehydrogenase N-terminal" evidence="10">
        <location>
            <begin position="17"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="Lactate/malate dehydrogenase C-terminal" evidence="11">
        <location>
            <begin position="158"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="188"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="23"/>
            <end position="28"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR22305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="326" mass="34986" checksum="69DCDE0965D6E7C9" modified="2009-07-28" version="1">MAQNKESSTMKAGLNPVKVVVVGTGSVGSTTAYTLLLSGMVAEIVLIDINKDKADGEGMDLNHAAPMTTDCRVYVGDYPDCANAAIVIITGGANQKPGQTRMDLAAKNAKIMQEIIPNIVKNAPDTILLLATNPVDVLTSISYKLSGFPASRVIGSGTLLDSARLRYNLSKYYNISSESIGAFIIGEHGDSELPVWSLASIAGMRLRDYCEKSNQKFDQDALEKIFEKTRSAAYDIIKRKGYTAYGIAAGLVRIVETILKNEGSLLTVSTVGDYFGVKQVALSVPTKVDRTGAHHIVGLSLDDNEIEEVKKSGQNIKSVCSRLGFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFZ2</accession>
    <name>C5DFZ2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0D01100p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0D01100g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the NFYC/HAP5 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552772.1">
        <property type="nucleotide sequence ID" value="XM_002552726.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFZ2"/>
    <dbReference type="FunCoup" id="C5DFZ2">
        <property type="interactions" value="576"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFZ2"/>
    <dbReference type="GeneID" id="8294992"/>
    <dbReference type="KEGG" id="lth:KLTH0D01100g"/>
    <dbReference type="eggNOG" id="KOG1657">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045277_5_2_1"/>
    <dbReference type="InParanoid" id="C5DFZ2"/>
    <dbReference type="OMA" id="MRAWCIA"/>
    <dbReference type="OrthoDB" id="1272441at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016602">
        <property type="term" value="C:CCAAT-binding factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22908">
        <property type="entry name" value="HFD_NFYC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000017">
        <property type="entry name" value="Ccaat-binding factor complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050568">
        <property type="entry name" value="Transcr_DNA_Rep_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10252">
        <property type="entry name" value="HISTONE-LIKE TRANSCRIPTION FACTOR CCAAT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10252:SF8">
        <property type="entry name" value="NUCLEAR TRANSCRIPTION FACTOR Y SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="8">
        <location>
            <begin position="40"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR22334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="138" mass="16056" checksum="56B44DA546A607CB" modified="2009-07-28" version="1">MDPHPEEEEIDVFRNVGQGLVGPYREITIQYWQELINEIEMTNEPGSPHQDDFKSHSLPLARIKKVMKTDEDVRMISAEAPILFAKACEIFITELTMRAWCIAEEHKRRTLQKSDIAQALLKSDMFDFLIDIVPRNIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-04-02" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>C5DG32</accession>
    <name>C5DG32_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0D02024p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0D02024g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552812.1">
        <property type="nucleotide sequence ID" value="XM_002552766.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DG32"/>
    <dbReference type="STRING" id="559295.C5DG32"/>
    <dbReference type="GeneID" id="8295032"/>
    <dbReference type="KEGG" id="lth:KLTH0D02024g"/>
    <dbReference type="eggNOG" id="ENOG502QQ7A">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016142_1_0_1"/>
    <dbReference type="InParanoid" id="C5DG32"/>
    <dbReference type="OMA" id="YICLRTH"/>
    <dbReference type="OrthoDB" id="2553626at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="258"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="299"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="385"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="497"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="593"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="61"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="385"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="405"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="439"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="506"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="638" mass="70777" checksum="177F65E8BD034AD3" modified="2009-07-28" version="1">MSFDWLNVPGLSGSGNENIEAAEPPPSVSFNFGNSASEPPTERESHVTMPQRADRADAFHSQSSYDSASSSAKSQARPDNYMETKEDLQVPLSLSRTQLTREEVRTYLRWYGYIASRKHTKLIALDDVFHFMTNFPLGQDVKERIAHIFKTCKNALNIGQFFAILRLIARALTDGVLPTRKMIMKAAPVPKPKPILAAGNEEVYEEIDDSQDGPELKVDFDSFASLLLTGQKKKRMRRRITNDHKRSKRVRFTDKLVTFQDHVQSPENEQGTEDEEKSGDNEPLDLSLPMDQLLKKMAARKKNNSALVSEPPSQKVPESQEEKEVLEDMKESLNHFHQIHNVDSVSLGGVPAQVPSAGTPSANATAQIEPLKPTATGSANYLFRSSQQQPQSVNQEPLQPLKPTATGSANYLFRSSQQQATSQKPEHNSFNFMHAVSPDPSNNAMHRNNTTFQSPTPEIPQIRAPLAANFAGQPLHSSSQAPSNLNVPFSNQSFLPPPSSGFGISPQPTSQNNFSPNLSANNYFQNLLSSSPSPNASTLQLPQSASVGPYANAYSSNSKVAQDSNYLKPNLTGPSNPQYQINPNQYQRREYQSFTPSPVPTLPSYPQAPQNTYKSHDYLGDLRALQEHVDQLQNAYNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>C5DG50</accession>
    <name>C5DG50_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0D02420p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0D02420g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552830.1">
        <property type="nucleotide sequence ID" value="XM_002552784.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DG50"/>
    <dbReference type="FunCoup" id="C5DG50">
        <property type="interactions" value="47"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DG50"/>
    <dbReference type="GeneID" id="8295050"/>
    <dbReference type="KEGG" id="lth:KLTH0D02420g"/>
    <dbReference type="eggNOG" id="ENOG502QVX4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062645_2_0_1"/>
    <dbReference type="InParanoid" id="C5DG50"/>
    <dbReference type="OMA" id="REFWPLF"/>
    <dbReference type="OrthoDB" id="10012223at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005619">
        <property type="term" value="C:ascospore wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005628">
        <property type="term" value="C:prospore membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052786">
        <property type="entry name" value="Spore_wall_assembly"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34292">
        <property type="entry name" value="OUTER SPORE WALL PROTEIN LDS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34292:SF3">
        <property type="entry name" value="OUTER SPORE WALL PROTEIN LDS2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="278"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="311" mass="34703" checksum="5A64E7565902B1D8" modified="2009-07-28" version="1">MSDAHLVAPLNNNSATAATTAPAEAEVHLVKRLRRETFVQALAVKLRRRVSYVRKNFLFGLFVDQTYVYPVLGFLRFVVTPSFWLYSFVLAFCFAFIFVTVAVLYYLFLLPILLIWAVSTLGPVGFVIVHIQWLLQSNALAITLTNILIAPIFADSIFDATLTKLGHAEFLERAKHLPVTSPKQASWRSLEFWLVVFPSKTVSLMFSLSSKVALSMLSLIPIVGPTIVNQLLSPTRGFSYGKRFYVLKNLSGSQLKDKFYEHLGQYTAFGMMAGLLELIPIMSIITIPSNVIAGALWASNELKKGEMSINA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DG55</accession>
    <name>C5DG55_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0D02530p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0D02530g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the phosphatase 2A regulatory subunit A family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552835.1">
        <property type="nucleotide sequence ID" value="XM_002552789.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DG55"/>
    <dbReference type="FunCoup" id="C5DG55">
        <property type="interactions" value="572"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DG55"/>
    <dbReference type="GeneID" id="8295055"/>
    <dbReference type="KEGG" id="lth:KLTH0D02530g"/>
    <dbReference type="eggNOG" id="KOG0211">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015533_2_1_1"/>
    <dbReference type="InParanoid" id="C5DG55"/>
    <dbReference type="OMA" id="NRVEAMQ"/>
    <dbReference type="OrthoDB" id="340346at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000062">
        <property type="entry name" value="Serine/threonine-protein phosphatase 2A regulatory subunit A alpha isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055231">
        <property type="entry name" value="2AA_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000357">
        <property type="entry name" value="HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054573">
        <property type="entry name" value="PP2A/SF3B1-like_HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051023">
        <property type="entry name" value="PP2A_Regulatory_Subunit_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10648:SF4">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2 (FORMERLY 2A), REGULATORY SUBUNIT A, BETA ISOFORM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10648">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE PP2A 65 KDA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02985">
        <property type="entry name" value="HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22646">
        <property type="entry name" value="PPP2R1A-like_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22956">
        <property type="entry name" value="VPS15-like_hel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="65"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="104"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="182"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatase PP2A regulatory subunit A/Splicing factor 3B subunit 1-like HEAT repeat" evidence="5">
        <location>
            <begin position="307"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="349"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="388"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="427"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatase 2A Regulatory Subunit A helical" evidence="6">
        <location>
            <begin position="509"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="546"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="591"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF22646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF22956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR22397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="622" mass="69699" checksum="16C253B29808465F" modified="2009-07-28" version="1">MSGNKQAKNKSGDKDDNGQNSEDLYPLALLMDELKHDDIANRVEAMKKLDTIALALGPQRTRDELVPFLTEVAQDDEDEVFAVLAEQLGKFVPFIGGAKFATLLLPALEILASTEETLVRDKAVESLNNIAQELSEEQLFHDFIPLIEHLATAEWFSSKVSSCGLFKSVLARVKDDKFRKELLALYLQLVQDDTPMVRRAAGKNLPVLIDLLTQNPDLSTSEDWDYISSMFQKIVSDTQDSVKFLAVDILVSILKFFNTKHDTTHSKDLLRSAIQLITDEAWRVRYMAADRFDDLASQFKSNPEYIEELSGPFLALCEDNESDVRKGVAKQLPGFSKLLGDPQVVLKKVLPAVQNLSMDESEIVRASLASEITNLAPLIPKQDAIDSLIPILLNMLKDEYPDVRLNIIAKLKVVNEVVGIDLLSESLLPAITELAKDVNWRVRMAIIDYIPLLAEQLGVQFFDQQLGDLCLSWLWDTVYSIRSAAVVNLKNLTKIFGSEWSSSKIISRLLKSDSQLLENFVYRITLLSALTELVPVVSTEVTTEKILPFINHLAEDSVPNIRFNVAKSYAVVVESLAEEKGKYKDLINKSIISSLEKLCQDSDIDVRYFASQSLEKCQNLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>C5DG64</accession>
    <name>C5DG64_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">cystathionine gamma-lyase</fullName>
            <ecNumber evidence="4">4.4.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Gamma-cystathionase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0D02728g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-homocysteine and L-serine: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the trans-sulfuration enzymes family.</text>
    </comment>
    <dbReference type="EC" id="4.4.1.1" evidence="4"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552844.1">
        <property type="nucleotide sequence ID" value="XM_002552798.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DG64"/>
    <dbReference type="FunCoup" id="C5DG64">
        <property type="interactions" value="513"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DG64"/>
    <dbReference type="GeneID" id="8295064"/>
    <dbReference type="KEGG" id="lth:KLTH0D02728g"/>
    <dbReference type="eggNOG" id="KOG0053">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018986_2_3_1"/>
    <dbReference type="InParanoid" id="C5DG64"/>
    <dbReference type="OMA" id="YKQDGVG"/>
    <dbReference type="OrthoDB" id="3512640at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004123">
        <property type="term" value="F:cystathionine gamma-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019343">
        <property type="term" value="P:cysteine biosynthetic process via cystathionine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00614">
        <property type="entry name" value="CGS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000043">
        <property type="entry name" value="Cystathionine gamma-lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000008">
        <property type="entry name" value="Cystathionine gamma-synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054542">
        <property type="entry name" value="Cys_met_metab_PP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808:SF15">
        <property type="entry name" value="CYSTATHIONINE GAMMA-LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808">
        <property type="entry name" value="TRANS-SULFURATION ENZYME FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001434">
        <property type="entry name" value="CGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00868">
        <property type="entry name" value="CYS_MET_METAB_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0198">Cysteine biosynthesis</keyword>
    <keyword evidence="5 8 9" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="8">
        <location>
            <position position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001434-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR22406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="392" mass="42149" checksum="D0F95C4E056A2B5F" modified="2009-07-28" version="1">MTVLPSDKFATLAIHAGAHTDVHGSVIEPISLSTTFKQSAPAQPIGAYEYSRSQNPNRANLEAAVAALERAQFGLAFSSGSATTAVVLQSLPTDSHAISIGDVYGGTHRYFTQVAHAHGVQTSFTNDLERDLPQLVRPNTRLVWLESPTNPTLKVTDIRRVADAAHAANPDILVVVDNTFLSPYISNPLTHGADIVVHSATKYINGHSDVVLGVLATNNEPLYERLRFLQNAIGAVPSPFDSWLTHRGLKTLHLRVRAAAQSAQRIAKFLEASDRVEHVNYPGLSSHPAYAVVQKQHRDALGGGMISFRIKGGAGAAARFTSTTRLFTLAESLGGIESLVEVPAVMTHGGIPREQREASGVFDNLIRLSVGIEDIDDLLEDVRQALEKAGAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGC2</accession>
    <name>C5DGC2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0D04114p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0D04114g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552902.1">
        <property type="nucleotide sequence ID" value="XM_002552856.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGC2"/>
    <dbReference type="GeneID" id="8295127"/>
    <dbReference type="KEGG" id="lth:KLTH0D04114g"/>
    <dbReference type="eggNOG" id="ENOG502S3YH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_150213_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGC2"/>
    <dbReference type="OMA" id="RAYAKSW"/>
    <dbReference type="OrthoDB" id="10008801at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR22464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="141" mass="15507" checksum="F40B11F155A710FE" modified="2009-07-28" version="1">MFKTLLRSASSVPFRQPALPRAIVQTPRSLFYAKRCYAKSWDGNNSSPDVEAHLKVQKLLDDIQQHPGVAAKLHAVSEIMVSKGLANESAPPGPWQVVKILTDKDVRKAMGEFKAELEKSGIELGPDQLGPLMTVLGMEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGF5</accession>
    <name>C5DGF5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Alkyl transferase</fullName>
            <ecNumber evidence="3">2.5.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0D04906g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the UPP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.-" evidence="3"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552935.1">
        <property type="nucleotide sequence ID" value="XM_002552889.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGF5"/>
    <dbReference type="FunCoup" id="C5DGF5">
        <property type="interactions" value="48"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGF5"/>
    <dbReference type="GeneID" id="8295162"/>
    <dbReference type="KEGG" id="lth:KLTH0D04906g"/>
    <dbReference type="eggNOG" id="KOG1602">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038505_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGF5"/>
    <dbReference type="OMA" id="TKGQPDP"/>
    <dbReference type="OrthoDB" id="4173905at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904423">
        <property type="term" value="C:dehydrodolichyl diphosphate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045547">
        <property type="term" value="F:ditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyl diphosphate specific] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016094">
        <property type="term" value="P:polyprenol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00475">
        <property type="entry name" value="Cis_IPPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1180.10:FF:000005">
        <property type="entry name" value="Alkyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1180.10">
        <property type="entry name" value="Decaprenyl diphosphate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001441">
        <property type="entry name" value="UPP_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018520">
        <property type="entry name" value="UPP_synth-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036424">
        <property type="entry name" value="UPP_synth-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00055">
        <property type="entry name" value="uppS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291:SF2">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE COMPLEX SUBUNIT SRT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01255">
        <property type="entry name" value="Prenyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64005">
        <property type="entry name" value="Undecaprenyl diphosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01066">
        <property type="entry name" value="UPP_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="52"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU363018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR22497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="362" mass="41487" checksum="79A6A670520E6A58" modified="2009-07-28" version="1">MVEHNRSSNTVDVIEKELKQTKERFKPKAPPFPYVLSPMSILEYAHAQKLRLWSFILSLACVSWALLQIQMTLTNILKMGPLPEHVSFIMDGNRRYAKSLSLPVKSGHEAGAITLLNLLTVCRALGVKTVSAYAFSIENFNRPQEEVDTLTSLLAEKLDEVARRAQDCNSELFGLRLCVVGERSRISKELNDRISSVERMTSEGDSMMLYICFPYTSRNDIYHAIYDCAETAKYHGFPAAQIDESVLTNHMMLRENSNNCDLLVRTSGHTRLSDYMLWQVHQNGHVEFSSTLWPDYGFLEFFRVLLKWSFFTSVQRRRNSRRLLRLHTACHLKETLFPRAPKHVSIEDLPSPPQAISVADRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGR4</accession>
    <name>C5DGR4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0D07458p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0D07458g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NifU family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553044.1">
        <property type="nucleotide sequence ID" value="XM_002552998.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGR4"/>
    <dbReference type="FunCoup" id="C5DGR4">
        <property type="interactions" value="362"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGR4"/>
    <dbReference type="GeneID" id="8295276"/>
    <dbReference type="KEGG" id="lth:KLTH0D07458g"/>
    <dbReference type="eggNOG" id="KOG2358">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060555_0_2_1"/>
    <dbReference type="InParanoid" id="C5DGR4"/>
    <dbReference type="OMA" id="AIMEHYM"/>
    <dbReference type="OrthoDB" id="565552at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.130:FF:000001">
        <property type="entry name" value="NFU1 iron-sulfur cluster scaffold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="entry name" value="Fe-S cluster assembly (FSCA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.70">
        <property type="entry name" value="Scaffold protein Nfu/NifU, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014824">
        <property type="entry name" value="Nfu/NifU_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036498">
        <property type="entry name" value="Nfu/NifU_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035433">
        <property type="entry name" value="NFU1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001075">
        <property type="entry name" value="NIF_FeS_clus_asmbl_NifU_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11178">
        <property type="entry name" value="IRON-SULFUR CLUSTER SCAFFOLD PROTEIN NFU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11178:SF1">
        <property type="entry name" value="NFU1 IRON-SULFUR CLUSTER SCAFFOLD HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08712">
        <property type="entry name" value="Nfu_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01106">
        <property type="entry name" value="NifU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036773">
        <property type="entry name" value="HIRIP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00932">
        <property type="entry name" value="Nfu_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="Fe-S cluster assembly (FSCA) domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110836">
        <property type="entry name" value="Hypothetical protein SAV1430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Scaffold protein Nfu/NifU N-terminal" evidence="2">
        <location>
            <begin position="23"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR22606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="248" mass="27879" checksum="C855E45759AFE3AA" modified="2009-07-28" version="1">MFVARRIQTVKKSLGCGSQFLHIKSVSTPNENALKFISTDGELLQERGKPSVEIKNTDESLIKHSPLASRLFTQCPGVEALMIGDDFITVNKDEMVHWNQITPSVIDLLTQYLASGREAVTPEFFSVQEQGVGYDVNVPKFEYNEDEQEISDMIDELIQTRIRPAIMDDGGDIQYRGYDPQTGTVYLKLQGACKSCSSSEVTLKHGIESMLKHYIEEVENVVQILDPEEQVALKEFEKLEKKLESTAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGR5</accession>
    <name>C5DGR5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">4a-hydroxytetrahydrobiopterin dehydratase</fullName>
            <ecNumber evidence="3">4.2.1.96</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">4-alpha-hydroxy-tetrahydropterin dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0D07480g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(4aS,6R)-4a-hydroxy-L-erythro-5,6,7,8-tetrahydrobiopterin = (6R)-L-erythro-6,7-dihydrobiopterin + H2O</text>
            <dbReference type="Rhea" id="RHEA:11920"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15642"/>
            <dbReference type="ChEBI" id="CHEBI:43120"/>
            <dbReference type="EC" id="4.2.1.96"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the pterin-4-alpha-carbinolamine dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.96" evidence="3"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553045.1">
        <property type="nucleotide sequence ID" value="XM_002552999.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGR5"/>
    <dbReference type="FunCoup" id="C5DGR5">
        <property type="interactions" value="48"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGR5"/>
    <dbReference type="GeneID" id="8295277"/>
    <dbReference type="KEGG" id="lth:KLTH0D07480g"/>
    <dbReference type="eggNOG" id="KOG4073">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081974_4_0_1"/>
    <dbReference type="InParanoid" id="C5DGR5"/>
    <dbReference type="OMA" id="IAMRSHL"/>
    <dbReference type="OrthoDB" id="277398at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008124">
        <property type="term" value="F:4-alpha-hydroxytetrahydrobiopterin dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006729">
        <property type="term" value="P:tetrahydrobiopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00488">
        <property type="entry name" value="PCD_DCoH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.20">
        <property type="entry name" value="Transcriptional coactivator/pterin dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036428">
        <property type="entry name" value="PCD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001533">
        <property type="entry name" value="Pterin_deHydtase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12599">
        <property type="entry name" value="PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12599:SF0">
        <property type="entry name" value="PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01329">
        <property type="entry name" value="Pterin_4a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55248">
        <property type="entry name" value="PCD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00030497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR22607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="109" mass="12425" checksum="A1CFAA4EF2C396DE" modified="2009-07-28" version="1">MYNKVKRVAAFPLSTAEISKHLVKHPKWSYDGLRISRDVAFKDFETTWSFLGQLAMRSHLWGHHPTITTTYNKVNLRLTTHDVNGVSDIDIKLMERIEGYIAGHGEASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>C5DH43</accession>
    <name>C5DH43_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="13">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">KLTH0E01210g</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the helicase family. RAD25/XPB subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="13"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553541.1">
        <property type="nucleotide sequence ID" value="XM_002553495.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DH43"/>
    <dbReference type="FunCoup" id="C5DH43">
        <property type="interactions" value="1083"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DH43"/>
    <dbReference type="GeneID" id="8291679"/>
    <dbReference type="KEGG" id="lth:KLTH0E01210g"/>
    <dbReference type="eggNOG" id="KOG1123">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008213_0_0_1"/>
    <dbReference type="InParanoid" id="C5DH43"/>
    <dbReference type="OMA" id="RCQEIDY"/>
    <dbReference type="OrthoDB" id="10262986at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000112">
        <property type="term" value="C:nucleotide-excision repair factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005675">
        <property type="term" value="C:transcription factor TFIIH holo complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18029">
        <property type="entry name" value="DEXHc_XPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18789">
        <property type="entry name" value="SF2_C_XPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000077">
        <property type="entry name" value="Probable DNA repair helicase RAD25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000117">
        <property type="entry name" value="Putative DNA repair helicase rad25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050615">
        <property type="entry name" value="ATP-dep_DNA_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032438">
        <property type="entry name" value="ERCC3_RAD25_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001161">
        <property type="entry name" value="XPB/Ssl2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032830">
        <property type="entry name" value="XPB/Ssl2_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00603">
        <property type="entry name" value="rad25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11274:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION AND DNA REPAIR FACTOR IIH HELICASE SUBUNIT XPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11274">
        <property type="entry name" value="RAD25/XP-B DNA REPAIR HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16203">
        <property type="entry name" value="ERCC3_RAD25_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13625">
        <property type="entry name" value="Helicase_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00851">
        <property type="entry name" value="XRODRMPGMNTB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="16">
        <location>
            <begin position="369"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="17">
        <location>
            <begin position="585"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="34"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="254"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="775"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="34"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="55"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="264"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="784"/>
            <end position="793"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAR23104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="839" mass="94616" checksum="C3D34FCF632EC904" modified="2009-07-28" version="1">MAEHQYQTRSKGGLYSNTDLNYFSDDSVATDNSDYDDGFIDGDDDMGMTNASSRPKPKKQVRKTNQKNKKNNSSTNETLNKLAAKDQTFLHDVQTDAPPDFVPDVVSGLFRRNDFSYLKLKPDHASRPLWISPGDGRVILESFSPLAEQAQDFLVTIAEPVSRPSHIHEYKITAYSLYAAVSVGLETDDIIAVLDRLSKVPVAPSIINFIKSATVSYGKVKLVIKHNRYFVETSQADILQMLLKDPVIGSLRIDSDATPAPNKAGSQQASRPNESAPSEKVDPNDVEAVFSSVVGEIRLDDDNDDIDAVHAFEIANESVEIVKKRCQEIDYPVLEEYDFRNDHRNPDLDIDLKPSTQIRPYQEKSLSKMFGNGRARSGIIVLPCGAGKTLVGITAACTIKKSVIVLCTSSVSVMQWRQQFLQWCTLQPENVAVFTSDNKEMFQTESGLVVSTYSMVANTRNRSHDSQKVMDFLTGREWGFIILDEVHVVPAAMFRRVVTTIAAHAKLGLTATLVREDDKISDLNFLIGPKLYEANWMELSQKGHIANVQCAEVWCPMTAEFYQEYLRENARKRMLLYIMNPTKFQACQFLIQYHEKRGDKIIVFSDNVYALQEYALKLGKPFIYGSTPQQERMNILQNFQFNDQINTIFLSKVGDTSIDLPEATCLIQISSHYGSRRQEAQRLGRILRAKRRNDEGFNAFFYSLVSKDTQEMYYSTKRQAFLVDQGYAFKVITHLHGMESLPNLTYSTARERRELLQEVLLKNEEAAGIEVGDDAENSVGRGAHANKKAKSKAVRGEGSLSGLAGGEDMAYVEYSSNRNKDLKEHHPMIRKLYYRNAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DH77</accession>
    <name>C5DH77_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0E01980p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0E01980g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553575.1">
        <property type="nucleotide sequence ID" value="XM_002553529.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DH77"/>
    <dbReference type="FunCoup" id="C5DH77">
        <property type="interactions" value="165"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DH77"/>
    <dbReference type="GeneID" id="8291714"/>
    <dbReference type="KEGG" id="lth:KLTH0E01980g"/>
    <dbReference type="eggNOG" id="KOG0876">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057349_1_1_1"/>
    <dbReference type="InParanoid" id="C5DH77"/>
    <dbReference type="OMA" id="MTAREPN"/>
    <dbReference type="OrthoDB" id="275227at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.55.40.20">
        <property type="entry name" value="Iron/manganese superoxide dismutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019832">
        <property type="entry name" value="Mn/Fe_SOD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036324">
        <property type="entry name" value="Mn/Fe_SOD_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036314">
        <property type="entry name" value="SOD_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43595">
        <property type="entry name" value="37S RIBOSOMAL PROTEIN S26, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43595:SF2">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02777">
        <property type="entry name" value="Sod_Fe_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54719">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46609">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Manganese/iron superoxide dismutase C-terminal" evidence="2">
        <location>
            <begin position="111"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="Manganese/iron superoxide dismutase C-terminal" evidence="2">
        <location>
            <begin position="187"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00037226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR23138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="252" mass="28749" checksum="9FBF62B3832284A4" modified="2009-07-28" version="1">MFRFASKRSIHSVPKLKNSGHVLEQGIPNVLSASGFNIAWTQQQKYLCDKLTLATAGTSQESYLPFHLILNTAKKPFQTNIFNLASAAHNNHLFVENVLPGPANTAPSRLFRTRIEEQYNCTWEEFKTEMVRRAETDVVGQGWLFLVENSDKELHILTVQNNGTPYYFPRNQSFDLNSALKLEEYSQLDAVRTIVKKGGKIQDWTMPLIAVSLWDHAYLHDYGVKGRSQYVKNVLDNLNWTVVNNRLYTGPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHB0</accession>
    <name>C5DHB0_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">arginine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Arginyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0E02816g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>tRNA(Arg) + L-arginine + ATP = L-arginyl-tRNA(Arg) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:20301"/>
            <dbReference type="Rhea" id="RHEA-COMP:9658"/>
            <dbReference type="Rhea" id="RHEA-COMP:9673"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78513"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.19"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.19" evidence="2"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553608.1">
        <property type="nucleotide sequence ID" value="XM_002553562.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHB0"/>
    <dbReference type="FunCoup" id="C5DHB0">
        <property type="interactions" value="1074"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHB0"/>
    <dbReference type="GeneID" id="8291752"/>
    <dbReference type="KEGG" id="lth:KLTH0E02816g"/>
    <dbReference type="eggNOG" id="KOG1195">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006406_6_2_1"/>
    <dbReference type="InParanoid" id="C5DHB0"/>
    <dbReference type="OMA" id="YEFKWER"/>
    <dbReference type="OrthoDB" id="68056at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004814">
        <property type="term" value="F:arginine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006420">
        <property type="term" value="P:arginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07956">
        <property type="entry name" value="Anticodon_Ia_Arg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00671">
        <property type="entry name" value="ArgRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.70:FF:000006">
        <property type="entry name" value="Arginyl-tRNA synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000006">
        <property type="entry name" value="Arginyl-tRNA synthetase 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000058">
        <property type="entry name" value="Mitochondrial arginyl-tRNA synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.70">
        <property type="entry name" value="Arginyl tRNA synthetase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00123">
        <property type="entry name" value="Arg_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001278">
        <property type="entry name" value="Arg-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005148">
        <property type="entry name" value="Arg-tRNA-synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036695">
        <property type="entry name" value="Arg-tRNA-synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035684">
        <property type="entry name" value="ArgRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008909">
        <property type="entry name" value="DALR_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00456">
        <property type="entry name" value="argS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11956:SF11">
        <property type="entry name" value="ARGININE--TRNA LIGASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11956">
        <property type="entry name" value="ARGINYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03485">
        <property type="entry name" value="Arg_tRNA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05746">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00750">
        <property type="entry name" value="tRNA-synt_1d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01038">
        <property type="entry name" value="TRNASYNTHARG"/>
    </dbReference>
    <dbReference type="SMART" id="SM01016">
        <property type="entry name" value="Arg_tRNA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00836">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55190">
        <property type="entry name" value="Arginyl-tRNA synthetase (ArgRS), N-terminal 'additional' domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arginyl tRNA synthetase N-terminal" evidence="12">
        <location>
            <begin position="71"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="DALR anticodon binding" evidence="11">
        <location>
            <begin position="527"/>
            <end position="645"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR23171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="645" mass="73753" checksum="0403411623E860D2" modified="2009-07-28" version="1">MLRRAVNFVAISRSFQVRLKQHRIWRLQGLRYFSHQPEIMSATSITSQLQKLSIQEPPALEGSFPNVNVVDLMRNFISVELSKISGVDTQLIYPALEWTNTLERGDLLIPVPRLRIKGSNPKDLATEWAEKFPLGNFLTKVEANGPFVQFFFKPDFLFKVVLPDILTRKEEYGSAPLVQNKKVVIEFSSPNIAKPFHAGHLRSTIIGGFLSNLYEKMGWDVVRLNYLGDWGKQFGVLAVGFERYGDEEALSKDPIKHLYDVYVRVNQDITAEGDSLPEDKSTDGQARAYFKKMEDGDENALKIWKRFRELSIEKYIDTYARLNIKYDSYSGESQASRESMQLALDMFREKGLTHEDRGALLIDLTKFNKKLGKTIVQKSDGTTLYMTRDVGTAMDRHKKYNFDKMIYVIACQQDLYTAQLFEVLKQLGFGWAKDLQHVNFGMVQGMSTRKGTVVFLDNILEETKEKMHEVMKTNKAKYAQIENPDEVADLVGISAVMIQDMQAKRINNYEFKWERMTSFEGDTGPYLQYAHSRLRSVERNASEITQEKMVNADFSLLHEPAAVLLVRLLAQYPDVLRNAIKTHEPATVVTYLFKLTHQVSSCYDVLWVAGQTPELAAARLALYASARQVLYNGMTLLGLTPVDRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHN3</accession>
    <name>C5DHN3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 18">Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial</fullName>
            <ecNumber evidence="5 18">1.3.5.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ordered locus">KLTH0E05742g</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a quinone + succinate = fumarate + a quinol</text>
            <dbReference type="Rhea" id="RHEA:40523"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.3.5.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
        <text evidence="18">Binds 1 [2Fe-2S] cluster.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18">
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </cofactor>
        <text evidence="18">Binds 1 [3Fe-4S] cluster.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="18">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 18">Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (eukaryal route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of complex II composed of four subunits: a flavoprotein (FP), an iron-sulfur protein (IP), and a cytochrome b composed of a large and a small subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 18">Mitochondrion inner membrane</location>
            <topology evidence="1 18">Peripheral membrane protein</topology>
            <orientation evidence="1 18">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family.</text>
    </comment>
    <dbReference type="EC" id="1.3.5.1" evidence="5 18"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553731.1">
        <property type="nucleotide sequence ID" value="XM_002553685.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHN3"/>
    <dbReference type="FunCoup" id="C5DHN3">
        <property type="interactions" value="754"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHN3"/>
    <dbReference type="GeneID" id="8291884"/>
    <dbReference type="KEGG" id="lth:KLTH0E05742g"/>
    <dbReference type="eggNOG" id="KOG3049">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044838_0_0_1"/>
    <dbReference type="InParanoid" id="C5DHN3"/>
    <dbReference type="OMA" id="DGQYFGP"/>
    <dbReference type="OrthoDB" id="1696654at2759"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER01006"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051538">
        <property type="term" value="F:3 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008177">
        <property type="term" value="F:succinate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000007">
        <property type="entry name" value="Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1060.10:FF:000001">
        <property type="entry name" value="Succinate dehydrogenase iron-sulfur subunit SdhB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="entry name" value="Alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006058">
        <property type="entry name" value="2Fe2S_fd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050573">
        <property type="entry name" value="SDH/FRD_Iron-Sulfur"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004489">
        <property type="entry name" value="Succ_DH/fum_Rdtase_Fe-S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025192">
        <property type="entry name" value="Succ_DH/fum_Rdtase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00384">
        <property type="entry name" value="dhsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004616">
        <property type="entry name" value="PRK05950.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11921:SF29">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE [UBIQUINONE] IRON-SULFUR SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11921">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE IRON-SULFUR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13085">
        <property type="entry name" value="Fer2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13534">
        <property type="entry name" value="Fer4_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46548">
        <property type="entry name" value="alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00197">
        <property type="entry name" value="2FE2S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 18" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="16 18" id="KW-0003">3Fe-4S</keyword>
    <keyword evidence="8 18" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="12" id="KW-0249">Electron transport</keyword>
    <keyword evidence="14 18" id="KW-0408">Iron</keyword>
    <keyword evidence="15 18" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="18" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="18" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="19">
        <location>
            <begin position="33"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="20">
        <location>
            <begin position="166"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00016766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU361237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="CAR23294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="263" mass="30097" checksum="217E0E95A8162469" modified="2009-07-28" version="1">MMMSGILKRNIAGVVARRGLATEVVQEPRLKTFKIYRWNPDHPADKPKLQEFKVDLNKCGPMVLDALLKIKDEQDATLTLRRSCREGICGSCAMNIGGRNTLACLCKIDTDVSKQTKIYPLPHMYIVKDLVPDLTQFYKQYKSVQPYLQRSKMPEDGKENLQSIEDRKKLDGLYECILCACCSTSCPSYWWNQEQYLGPAVLMQAYRWLIDSRDEASKFRKEMLQNSMSLYRCHTIMNCTRTCPKGLNPGYAIAEIKKALATS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHN4</accession>
    <name>C5DHN4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Vacuolar protein sorting-associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0E05764g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Mediates the transfer of lipids between membranes at organelle contact sites. May play a role in mitochondrial lipid homeostasis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the VPS13 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553732.1">
        <property type="nucleotide sequence ID" value="XM_002553686.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DHN4">
        <property type="interactions" value="787"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHN4"/>
    <dbReference type="GeneID" id="8291885"/>
    <dbReference type="KEGG" id="lth:KLTH0E05764g"/>
    <dbReference type="eggNOG" id="KOG1809">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000135_0_0_1"/>
    <dbReference type="InParanoid" id="C5DHN4"/>
    <dbReference type="OMA" id="SGWRPIR"/>
    <dbReference type="OrthoDB" id="428159at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045324">
        <property type="term" value="P:late endosome to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045053">
        <property type="term" value="P:protein retention in Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026847">
        <property type="entry name" value="VPS13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056748">
        <property type="entry name" value="VPS13-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056747">
        <property type="entry name" value="VPS13-like_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017148">
        <property type="entry name" value="VPS13_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026854">
        <property type="entry name" value="VPS13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009543">
        <property type="entry name" value="VPS13_VAB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16166:SF93">
        <property type="entry name" value="INTERMEMBRANE LIPID TRANSFER PROTEIN VPS13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16166">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VPS13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25037">
        <property type="entry name" value="VPS13_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25033">
        <property type="entry name" value="VPS13_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12624">
        <property type="entry name" value="VPS13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25036">
        <property type="entry name" value="VPS13_VAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037235">
        <property type="entry name" value="VPS13_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="3 4" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Chorein N-terminal" evidence="5">
        <location>
            <begin position="1"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="domain" description="VPS13-like middle region" evidence="6">
        <location>
            <begin position="1118"/>
            <end position="1802"/>
        </location>
    </feature>
    <feature type="domain" description="Vacuolar protein sorting-associated protein 13 VPS13 adaptor binding" evidence="7">
        <location>
            <begin position="1868"/>
            <end position="2444"/>
        </location>
    </feature>
    <feature type="domain" description="Intermembrane lipid transfer protein VPS13-like C-terminal" evidence="8">
        <location>
            <begin position="2971"/>
            <end position="3076"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR037235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF25036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF25037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR23295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="3102" mass="351646" checksum="4029E5312B29843D" modified="2009-07-28" version="1">MLESLAAGLLNRFLGSYVENFDPKQLNVGIWSGDVKLKKLKLKKESLDALDLPIDVRFGFLGDLTLLVPWSSLKNKPVKILIEDVYLLCGPRDQSAKSFAEDEERELRLKLQKLDEYEALGKSQPTGKEDSSSSESFTQSLLTKVVDNLQISIKNIHIRYEDMDCVFGEVPYSLGATLSELSIVSTDEHWEPSFISITSSIARKLLTLNSLSVYWNTDCESVYHPDHDELFRRLRNSISSKKNIPDHEFLLRPVTGIGRLVLSKKGSTETNPHVDIALVFDEFGFDINNCQYEDILNTASKLHWYQKTLKSRKHRPNFPVSEDPKAWFKYAAKAVLQEVHEKNYKWSWDYIKWRRDRRIEYIPLWKKKLGTEKKTQSLSDEEQQKLKELHKELSFEDIKLFRALAKKSYLTEKLEVSNSAAESKGSSAAGSSGWFSSWWNGNSPQNDDDLVMSEEQQQELYDAIEFDENKQVIDSIDIPKDRITAKVTCVLNRGSLSIVDKNRSIKLGEMVFEGCETEFLQRPDSFFCSFKLHEFKVEDGSPNTLYKHIVSVKNLQMNEGNVSQHDEPFFKVAVEKNPLDDSADSKIDIKLRSMTIFYHVHFVNEIIKLFRPPRQHLDTIGAIMSAAEATVEGWTVQTRMGLESILEDHKTVNLSLDLQAPLLIIPLDPHVWASPCAVIDAGHMSIVSDLVPKETLTKYKNMSSSEYEKLDTRDIKRLMFDRFKLRLTDTQFLIGPDIRSTISNLNVSERESSFVILDKLEVELDVDVSILPKALNLPKIKIYGKLPLLKLSLNDYQYKVMMQLIDQCLPDLDYPESDLDSVDPKTSSDSKILNSLESKEVAILRQTLSRLQKMSEAELKQRLLQLTFDVEVIQLSIFKCNNAETMKSDLLVDLFGDRMHLTFAKKAQDMDLHLNLQSFKVVDHIDSGASSEFKNMIASSNENTQASHLFELKLNRAQRIVTYNNTLIEVFDQDVSLDMTELTFVLTPKSILTILNYTLTTFTNPEAPELPGDALKHNSPDLADAPQKINLRMNMDNIIVILNDDSVKLATFRLSAAQIEQLLLPEKMKINAKLGAMQLTNDADDGLSIDSPLRELISMSGSELAELTYETFDPSTEKLDYSSLLKYKTGSMNVNFVDHAVNKIVNYLAKFQKMKVFFDRAREAAYNQAGNIETVNNIKLDILVRAPVITFPKLIDPRRNLFDHVSFFLGEFFLQNSFHNEGERLVNKIKAGIRSGNLGSTFHMEHELTQNLHIIENLDLHFDIDHYQGTKQIPVKIDAKGVFEPLAAHLTELQLQYIYLLSQRLPNAFIIDYCEDDFAEIQDVARNANTFVSPEAKTETKPDPILFTEEEDRTSETEFSVNFTAPQLSLTIYNDTKNSSDISQSGITKISLNDIGLSLGIDKDSRMRAETHIASFTVDDIRKVKQNKHDKIIPKVSHDSYQFMASYTKEQHESLTVDNVNVTVDSPRIILALDYLFALKSFIDASVNINPPAKVFDKSPAHASPPALEQEQQPSSTKFQYSINVVDSSLVLLSDPSEENTEAIVFNVGQLLFADQNIMTASANNIGMFLCRMQSFDKNRIRLLDDFSSSILFDQRSSTSEKLITDVHASVEPLVMRLALRDIRLAMSIFNKAMTLAREHGLSPQVEPEADESGTKYGSFSKEFKKTLSRYAPSIVSSMSNISKSDDISEEELETIVKHEKLEANFEGLRLVLIGDIHELPIIDLSIKPFTLNAKDWTNDVEAVASIETYANVFNYSRSSWEPLLETVPLSFHLSKGIETDAALCFDIISRQLAEVSLSSRTIALLSQIPNSLYYTKDTEARGATKPYKICNDTGMDLKIWIASEKPQSKDQITILKSGETIPWEFEDWRKVRENLDTDDSMNTLGASIEGGHYESTLKIDVTSEGEDVYTLQPKVNGVHTRLACELKLCEDNVKLLIIRSTLTLENATSDLLEFKLSNEDGIFTINPGESRSIPAEKAYSSRFLLKPSGNYGWSRESISWRDLMSRPRSVVCENKEDPALFYYEVDAKFDKEEPLASIYPHMKVIISSPLILENLLPYSMKYKLFWRSESANATKGKEETETRLLHKGERVLIHSVTLRDYLLLAVQPMDDGLQMSEQSLINTRPNSELKAESKLFLKYENGQKLNLNLHYRTVEGSRAKIISVYSPYVILNGSNRDIQVEGDRNNIAISKVLLKDDSSKYTNPRMFSFERDRSNKNRARIKFKDSDWSVRTSFDAIGQSVDLTMDIPNKNQESNIGVVVSEGEGKYALSKIIKIMPRYILRNNLDVDLEVSEYGSSNVLTAFSKVSFPLYKMRNIVNKHLSIKFLGSQSEWSSPFMIKDIGSSYVKVLKNRSGHQLLKLDIVLEDSTIFISIDNADGEWPYSIRNFSDHEFIFYQRDPHTFDTEEDYDLYDDLGDMKYEALYYRVPPRSAMPYAWDYPAARQKKLVLVCRQRQREIQLAEIGNLRPMRIPSRNGNEAPAIVDLNVVADGPTQALIITNYRPELSLYKLKTGNTTSSSLSSDKGDRFEVRDEDKNVFTKVVVSFEGVGISLINTRLQELCYVTLNGLELRYNESDLYQTASVKLKWLQIDNQLFGGIFPNILYPTAIQKTAKELNNHPVLSGSVSKVKDDTHGVLYLKHATLLLQELTVQLDEDFLMALIDFTKVPGASWVNNVEDNMCTECTMLSYPSELQRNNDIYFEVFHLQPTMLHLSFVRTERLNVEGEKVDPQNTLMFFTNVLTMAFANINDAPIKLNSLFLDNAKVPLPMLMSAIQVHYGQQFFYQIHKILGSADFLGNPVGLFNNISSGVWDIFYEPYQGYMMNDRPQELGISIAKGGLSFVKKSVFGLSDSFARFTGSVAKGLSVATQDAQFQERRRLEQRKARNNKSINGFGSGATSFVNGVSSGLRGIAFDPYQGASKEGVPGFLKGLGKGLVGLPTKTAIGMLDLASNVSEGIRNTTTVMDGSLTTQVRLPRYIGYDKIIKPYNLRESQGQYWLKSANGGECITDQYLAHVVLPGKQLAVLVSLQHIIEVRLASLEIMWKVRYTDVRSMTLERAGLVIVLKGASSELFVPISDPEEKRFLYKNISVAVNEYNKYCQAEL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2010-10-05" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHP6</accession>
    <name>IRC19_LACTC</name>
    <protein>
        <recommendedName>
            <fullName>Increased recombination centers protein 19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">IRC19</name>
        <name type="synonym">RRG4</name>
        <name type="ordered locus">KLTH0E06028g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in sporulation and maintenance of the mitochondrial DNA. Is probably involved in a pathway contributing to genomic integrity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IRC19 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553744.1">
        <property type="nucleotide sequence ID" value="XM_002553698.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHP6"/>
    <dbReference type="FunCoup" id="C5DHP6">
        <property type="interactions" value="32"/>
    </dbReference>
    <dbReference type="GeneID" id="8291897"/>
    <dbReference type="KEGG" id="lth:KLTH0E06028g"/>
    <dbReference type="eggNOG" id="ENOG502S35W">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106818_0_0_1"/>
    <dbReference type="InParanoid" id="C5DHP6"/>
    <dbReference type="OMA" id="FMRLKPF"/>
    <dbReference type="OrthoDB" id="3991133at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030435">
        <property type="term" value="P:sporulation resulting in formation of a cellular spore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0749">Sporulation</keyword>
    <feature type="chain" id="PRO_0000399060" description="Increased recombination centers protein 19">
        <location>
            <begin position="1"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="204" mass="23856" checksum="5207F34FAC1D931E" modified="2009-07-28" version="1">MASRGITITTNKAITTSKSTLILKHDKFLPPRNMINEFPHDDVLRMCYRRHMRLKPFISQRSMIQTTYVDYVRYKYKNEDYPKKCRASGIAHKTPVQSVLQQAELSLRFCLQAVMQVKKGVSDERSVSNEIRLSRNMLKNILAIEHEKAKLIAQNPRQNYPILREAFSYISPTAHKSSLLLRFNALREFDRCLIRFNMCMGTKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHQ3</accession>
    <name>C5DHQ3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Iron-sulfur assembly protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0E06182g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Involved in the assembly of mitochondrial and cytoplasmic iron-sulfur proteins. Probably involved in the binding of an intermediate of Fe/S cluster assembly.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HesB/IscA family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553751.1">
        <property type="nucleotide sequence ID" value="XM_002553705.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHQ3"/>
    <dbReference type="FunCoup" id="C5DHQ3">
        <property type="interactions" value="212"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHQ3"/>
    <dbReference type="GeneID" id="8291904"/>
    <dbReference type="KEGG" id="lth:KLTH0E06182g"/>
    <dbReference type="eggNOG" id="KOG1120">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069054_0_1_1"/>
    <dbReference type="InParanoid" id="C5DHQ3"/>
    <dbReference type="OMA" id="AMSCHES"/>
    <dbReference type="OrthoDB" id="333486at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.300.12:FF:000001">
        <property type="entry name" value="Iron-binding protein IscA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.300.12">
        <property type="entry name" value="HesB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016092">
        <property type="entry name" value="ATAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000361">
        <property type="entry name" value="ATAP_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050322">
        <property type="entry name" value="Fe-S_cluster_asmbl/transfer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017870">
        <property type="entry name" value="FeS_cluster_insertion_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035903">
        <property type="entry name" value="HesB-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00049">
        <property type="entry name" value="iron-sulfur cluster assembly accessory protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10072:SF41">
        <property type="entry name" value="IRON-SULFUR CLUSTER ASSEMBLY 1 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10072">
        <property type="entry name" value="IRON-SULFUR CLUSTER ASSEMBLY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01521">
        <property type="entry name" value="Fe-S_biosyn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89360">
        <property type="entry name" value="HesB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01152">
        <property type="entry name" value="HESB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core" evidence="5">
        <location>
            <begin position="164"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="95"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="101"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="130"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00054873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00071673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR23314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="267" mass="30149" checksum="AD1CDD70CD20ED1A" modified="2009-07-28" version="1">MRFAMSCHESLAVRSLQAFRLFSSRSILLNSAPAQASHTENHTYRARNAKRFLQTNHLQRTLGNSGPERWSFDFNQEKKSAPFKKPALTPLKPFHQESKESTIQTAASPNASKWSTYSMPSKESLAKQQDQAKRQGQEIEKQLASEPKPVKKQKRRLRPRKALISLTPNAVDHLKLLLNQPEPKLIRVGVKNRGCSGLTYDLEYITAPGKFDEVVEQDGVRVILDSKALFSIVGSEMDWIDDRLSSRFVFKNPNSKGTCGCGESFMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIH4</accession>
    <name>C5DIH4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0E12540p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0E12540g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
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            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="USP_3"/>
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    </dbReference>
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    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
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            <end position="52"/>
        </location>
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    <feature type="domain" description="USP" evidence="3">
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            <begin position="71"/>
            <end position="584"/>
        </location>
    </feature>
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            <end position="169"/>
        </location>
    </feature>
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        <location>
            <begin position="555"/>
            <end position="694"/>
        </location>
    </feature>
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        <location>
            <begin position="120"/>
            <end position="129"/>
        </location>
    </feature>
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        <location>
            <begin position="131"/>
            <end position="145"/>
        </location>
    </feature>
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        <location>
            <begin position="555"/>
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        </location>
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        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAR23585.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
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</entry>
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        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
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            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR23655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="55" mass="5006" checksum="5581194B645C3775" modified="2009-07-28" version="1">MKFTTVFAAAAAVTVASAANSSNSSSSNAAPAAGSNAVGYTAAGAALAGAVALLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIV9</accession>
    <name>C5DIV9_LACTC</name>
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        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0E15576g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
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        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
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                <person name="Jauniaux N."/>
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                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
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            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the WD repeat MDV1/CAF4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_002554111.1"/>
    </dbReference>
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    <dbReference type="KEGG" id="lth:KLTH0E15576g"/>
    <dbReference type="eggNOG" id="KOG4155">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012350_1_0_1"/>
    <dbReference type="InParanoid" id="C5DIV9"/>
    <dbReference type="OMA" id="ERLRYMD"/>
    <dbReference type="OrthoDB" id="496at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000266">
        <property type="term" value="P:mitochondrial fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016559">
        <property type="term" value="P:peroxisome fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Mdv1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021061">
        <property type="entry name" value="Mt_division_protein_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19855:SF28">
        <property type="entry name" value="CCR4-ASSOCIATED FACTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19855">
        <property type="entry name" value="WD40 REPEAT PROTEIN 12, 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11542">
        <property type="entry name" value="Mdv1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 9" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="402"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="445"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="500"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="585"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="608"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="125"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="166"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="291"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="125"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="323"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR23720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="723" mass="80394" checksum="DCECBE812E1CBD8E" modified="2009-07-28" version="1">MGSESNNKDQLSQIKRAVSTTASALFGSQSMEDTILSYSSPYKKLLHQTITNSGSSSALTKLRSTKNPFKSEENKAFQDIYSASNGKSFFANQFSNTKTSFRVLSYLSDDLLYDIPTITSRGNRDKKLLTENGDSTRRSKKSHPEEPSLFQGFRSSLPIIDETIKAQGPSVQDSQEAPRLLSGSEGGSETEKDERFSLPGDFSWDELNESYSVADLSQTVQSITDNLDLLEIQKNLAASEIREIDLKLQKLKMVRDLVFQRVAKVEQNELFLEKHLALLKDRIDMIEEYGLEDTQSSDTAEKTPQVPEKQHQNNNSASSETSTESEDATDDDSGDDTSALLSQSIYQKIKKSPPNSKPRGSKNALGQKKLPPNLHLNHNRKRKTYPTLQQYYEPGTKISSFTKAHDDSITCLDFDMPFGTMCTAGKLDHSVKIWNLSKKTQIGTLPGHLASVSCMQMDESSSLITGGRDALLKLWDLDKAEEQYFEGVPEEDEELCLHTFDAHVDEITALHFEGNSLVSGSQDRTIRQWDLRTGKCLQTIDINFTNRGTSDGFSTISSNNPRSSVLLTHNDPPVIGALQCYDAAMATGTKDGIVRLWDLRMGQVVRTLEGHTDAVTGLQFDAVNLVTGSLDRSIRIWDLRTGTLADAFAYDSPVSSLQFDLDKIVVANNESMVRVYNRKEEAHWTCGEADAEASGTDVVDFVRYKNGYLVEGRTTGDVNTWAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIZ5</accession>
    <name>C5DIZ5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0E16390p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0E16390g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ERCC1/RAD10/SWI10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554193.1">
        <property type="nucleotide sequence ID" value="XM_002554147.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIZ5"/>
    <dbReference type="FunCoup" id="C5DIZ5">
        <property type="interactions" value="149"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIZ5"/>
    <dbReference type="GeneID" id="8292365"/>
    <dbReference type="KEGG" id="lth:KLTH0E16390g"/>
    <dbReference type="eggNOG" id="KOG2841">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1267070_0_0_1"/>
    <dbReference type="InParanoid" id="C5DIZ5"/>
    <dbReference type="OMA" id="ITKLCMF"/>
    <dbReference type="OrthoDB" id="10262814at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070522">
        <property type="term" value="C:ERCC4-ERCC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000110">
        <property type="term" value="C:nucleotide-excision repair factor 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006312">
        <property type="term" value="P:mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070914">
        <property type="term" value="P:UV-damage excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22325">
        <property type="entry name" value="ERCC1_C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10130:FF:000015">
        <property type="entry name" value="SsDNA endonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047260">
        <property type="entry name" value="ERCC1-like_central_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004579">
        <property type="entry name" value="ERCC1/RAD10/SWI10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00597">
        <property type="entry name" value="rad10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12749:SF0">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12749">
        <property type="entry name" value="EXCISION REPAIR CROSS-COMPLEMENTING 1 ERCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03834">
        <property type="entry name" value="Rad10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ERCC1-like central" evidence="8">
        <location>
            <begin position="86"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="22"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="57"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="35"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="58"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR23756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="204" mass="23322" checksum="14ACF1F97FC735B0" modified="2009-07-28" version="1">MSEGATTSFESILAGVQRLREGKAQAKDVQVQPQAERKPPEVRETQKRELIINAFNQRKQDFNRNEPSEVEDRKRKWGNASAQGKTVLVSSSQRGNPLLSSMANTNWRYVSSTGGNKVYYDYCVQGRNIIFLSLKYHKLHPEYISKKIQPLVKNKNNILICVVDVENSENILKDLNKACMFNGFALLLAFNFEQAAKYIIFMNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJ40</accession>
    <name>C5DJ40_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Translation machinery-associated protein 20</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0F13266g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 6">Involved in translation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 6">Belongs to the TMA20 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554766.1">
        <property type="nucleotide sequence ID" value="XM_002554720.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJ40"/>
    <dbReference type="FunCoup" id="C5DJ40">
        <property type="interactions" value="558"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJ40"/>
    <dbReference type="GeneID" id="8292990"/>
    <dbReference type="KEGG" id="lth:KLTH0F13266g"/>
    <dbReference type="eggNOG" id="KOG2523">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090468_0_1_1"/>
    <dbReference type="InParanoid" id="C5DJ40"/>
    <dbReference type="OMA" id="GVENIHY"/>
    <dbReference type="OrthoDB" id="10249667at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11609">
        <property type="entry name" value="MCT1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21155">
        <property type="entry name" value="PUA_MCTS-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.400.20:FF:000001">
        <property type="entry name" value="Malignant T-cell-amplified sequence 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.400.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016437">
        <property type="entry name" value="MCT-1/Tma20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041366">
        <property type="entry name" value="Pre-PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004521">
        <property type="entry name" value="Uncharacterised_CHP00451"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00451">
        <property type="entry name" value="unchar_dom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22798:SF0">
        <property type="entry name" value="MALIGNANT T-CELL-AMPLIFIED SEQUENCE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22798">
        <property type="entry name" value="MCT-1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17832">
        <property type="entry name" value="Pre-PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005067">
        <property type="entry name" value="Tma_RNA-bind_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PUA" evidence="7">
        <location>
            <begin position="88"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00060251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00061046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00070056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR005067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR24329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="178" mass="19835" checksum="DCA9A38C926D4B8E" modified="2009-07-28" version="1">MFRKEDVHSRSNVKSSVQRTLKTKLVSQFPKLEEVIDELVPKKSHVELIKCEDKIQLYAVDGEVCFFQKFDELIPTLKLVHKFPEAFPTVQVDRGAIKFVLSGANIMCPGLTSPGAQLPEAPGLEKDALVIVKAENKESALAVGRLLMSTEDIKSINKGHGVEMIHHLGDPLWTFSVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJ65</accession>
    <name>C5DJ65_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">KLTH0F13882p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0F13882g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAPRE family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554791.1">
        <property type="nucleotide sequence ID" value="XM_002554745.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJ65"/>
    <dbReference type="FunCoup" id="C5DJ65">
        <property type="interactions" value="759"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJ65"/>
    <dbReference type="GeneID" id="8293017"/>
    <dbReference type="KEGG" id="lth:KLTH0F13882g"/>
    <dbReference type="eggNOG" id="KOG3000">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041744_2_0_1"/>
    <dbReference type="InParanoid" id="C5DJ65"/>
    <dbReference type="OMA" id="WIKRFWD"/>
    <dbReference type="OrthoDB" id="2119228at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035371">
        <property type="term" value="C:microtubule plus-end"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051233">
        <property type="term" value="C:spindle midzone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051010">
        <property type="term" value="F:microtubule plus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030473">
        <property type="term" value="P:nuclear migration along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035372">
        <property type="term" value="P:protein localization to microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000028">
        <property type="entry name" value="RP/EB family microtubule-associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004953">
        <property type="entry name" value="EB1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036133">
        <property type="entry name" value="EB1_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027328">
        <property type="entry name" value="MAPRE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10623">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEIN RP/EB FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03271">
        <property type="entry name" value="EB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140612">
        <property type="entry name" value="EB1 dimerisation domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51230">
        <property type="entry name" value="EB1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5 9" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="12">
        <location>
            <begin position="5"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="EB1 C-terminal" evidence="13">
        <location>
            <begin position="192"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="126"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="179"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="133"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR24354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="316" mass="35818" checksum="638BABDB678CF169" modified="2009-07-28" version="1">MSGVGESRSELLSWLNELLKLNYVKIEQCGTGAAYCQIIDSIYRDVPMHRVKFNATAEYEFLTNFKILQSSFTKHRLEKTILVEKLVRCRFQDNLEFLQWLKKFWVQNKDESEYDAESRRKYRAPAPAGGAALSQSAPSLSGGASSVVKRRTGGALRGSTAVPPRTAALGRSSAPLATRKAFNEQLLNTQAQLSQTQQDMEKLQKEAAQYREAMAIMERERDFYFGKLRDIEILSQSTKDLCKEGVYSSDPNELVRFLDKVQQILYATEEGFEVVEEYPSDGNLRDPVINNISTAPDPMEVKHQPAHNLITDEETF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJ68</accession>
    <name>C5DJ68_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit protein uS5m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0F13948g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the universal ribosomal protein uS5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554794.1">
        <property type="nucleotide sequence ID" value="XM_002554748.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJ68"/>
    <dbReference type="FunCoup" id="C5DJ68">
        <property type="interactions" value="670"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJ68"/>
    <dbReference type="GeneID" id="8293020"/>
    <dbReference type="KEGG" id="lth:KLTH0F13948g"/>
    <dbReference type="eggNOG" id="KOG2646">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037994_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJ68"/>
    <dbReference type="OMA" id="YWDYKPG"/>
    <dbReference type="OrthoDB" id="309483at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000022">
        <property type="entry name" value="28S ribosomal protein S5, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000041">
        <property type="entry name" value="37S ribosomal protein S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000851">
        <property type="entry name" value="Ribosomal_uS5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005324">
        <property type="entry name" value="Ribosomal_uS5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013810">
        <property type="entry name" value="Ribosomal_uS5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018192">
        <property type="entry name" value="Ribosomal_uS5_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48277">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOMAL PROTEIN S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48277:SF1">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOMAL PROTEIN S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00333">
        <property type="entry name" value="Ribosomal_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03719">
        <property type="entry name" value="Ribosomal_S5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00585">
        <property type="entry name" value="RIBOSOMAL_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50881">
        <property type="entry name" value="S5_DSRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 8 9" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="S5 DRBM" evidence="10">
        <location>
            <begin position="154"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR24357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="317" mass="35072" checksum="6E8C4FB6D7E17FB8" modified="2009-07-28" version="1">MLRRTFSTGLRALQQLPKQPPAAPAAQASHADFLAKYYPQELVRSIQLAESVIPADTAFRVSSNVEFAPAYLDDFSKLDSYWDYLPGSPNVHSQGPSTQFNFDSIQQPLPNGQEIPPGATVPRLDGGRSSATADIARGLQKQTGLDADYISRKLTMRPLVMKRVSNQTGKGKIPSFYALVVVGDRNGMVGLGEGKSRDEMSKAVSKAHWDAVRNLKQIPRYENRTIFGDIDHRYHGVKLFLRSVKPGFGLRVNHIIFEICECAGIKDLSGKVYKSRNEMNIAKGTLEALTEGQKTLDEIALGRGKKVVDVRKVYYSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJ95</accession>
    <name>C5DJ95_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">asparagine synthase (glutamine-hydrolyzing)</fullName>
            <ecNumber evidence="2">6.3.5.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0F14630g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-aspartate + L-glutamine + ATP + H2O = L-asparagine + L-glutamate + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12228"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (L-Gln route): step 1/1.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.4" evidence="2"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554821.1">
        <property type="nucleotide sequence ID" value="XM_002554775.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJ95"/>
    <dbReference type="FunCoup" id="C5DJ95">
        <property type="interactions" value="634"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJ95"/>
    <dbReference type="GeneID" id="8293297"/>
    <dbReference type="KEGG" id="lth:KLTH0F14630g"/>
    <dbReference type="eggNOG" id="KOG0571">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014658_2_2_1"/>
    <dbReference type="InParanoid" id="C5DJ95"/>
    <dbReference type="OMA" id="GIVCAFD"/>
    <dbReference type="OrthoDB" id="409189at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004066">
        <property type="term" value="F:asparagine synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006529">
        <property type="term" value="P:asparagine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01991">
        <property type="entry name" value="Asn_synthase_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00712">
        <property type="entry name" value="AsnB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000031">
        <property type="entry name" value="Asparagine synthase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000050">
        <property type="entry name" value="Asparagine synthetase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006426">
        <property type="entry name" value="Asn_synth_AEB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001962">
        <property type="entry name" value="Asn_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050795">
        <property type="entry name" value="Asn_Synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033738">
        <property type="entry name" value="AsnB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01536">
        <property type="entry name" value="asn_synth_AEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006949">
        <property type="entry name" value="PRK09431.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11772">
        <property type="entry name" value="ASPARAGINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11772:SF2">
        <property type="entry name" value="ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00733">
        <property type="entry name" value="Asn_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13537">
        <property type="entry name" value="GATase_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001589">
        <property type="entry name" value="Asn_synthetase_glu-h"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 11" id="KW-0061">Asparagine biosynthesis</keyword>
    <keyword evidence="6 10 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 11" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 10 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="14">
        <location>
            <begin position="2"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="active site" description="For GATase activity" evidence="11">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="366"/>
            <end position="367"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for beta-aspartyl-AMP intermediate formation" evidence="13">
        <location>
            <position position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR001589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR24384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="573" mass="64736" checksum="E659737542E604C9" modified="2009-07-28" version="1">MCGIFTCFRHQSVEEFKPDALRLVKRIRHRGPDWSGNIIKNSTLMAHERLAIVGLDSGAQPFVSEDNNYILGVNGEVYNHIQLREEFSSYPFKTLSDCEPIIPLYEKYGIDAPKYLDGMFAWALYDAKKDRIVAARDPIGITTLYMGRDSKSPKTVYFASELKCLTDNCDSIIAFPPGHVYDSETDQITRYFNPDWIDEDRIPSEPLDLLKIRHSLEKAVRKRLMAEVPYGVLLSGGLDSSLIASIAARETEKANSQMDPVKYDSEDKHLAGVDDAGNLRSAGWSHLHSFAIGLPNAPDLQAARKVAKFIGSIHHEHTFTLQEGLDALSDVIYHLETYDVTTIRASTPMFLLSRKIKAQGVKMVLSGEGSDEIFGGYLYFAQAPSAAEFHTESVKRVKNLHLADCLRANKSTMAWGLEARVPFLDREFLQLCMNLDPKEKMINAAEGRIEKYILRKAFDTSDEPGAKPYLPEEILWRQKEQFSDGVGYSWIDGLKDAAEAAISDEQFKQPKAEWGDDIPTTKEAYWYRLKFDALFPQKTAASTVMRWIPKADWGCAEDPSGRYAKIHEQHIDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJ97</accession>
    <name>C5DJ97_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">KLTH0F14674p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0F14674g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554823.1">
        <property type="nucleotide sequence ID" value="XM_002554777.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJ97"/>
    <dbReference type="FunCoup" id="C5DJ97">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJ97"/>
    <dbReference type="GeneID" id="8293052"/>
    <dbReference type="KEGG" id="lth:KLTH0F14674g"/>
    <dbReference type="eggNOG" id="KOG0236">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003182_0_2_1"/>
    <dbReference type="InParanoid" id="C5DJ97"/>
    <dbReference type="OMA" id="IEITPFY"/>
    <dbReference type="OrthoDB" id="409725at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034490">
        <property type="term" value="P:basic amino acid transmembrane import into vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07042">
        <property type="entry name" value="STAS_SulP_like_sulfate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.750.24:FF:000012">
        <property type="entry name" value="Sulfate transporter family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="entry name" value="STAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052706">
        <property type="entry name" value="Membrane-Transporter-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43310:SF4">
        <property type="entry name" value="AFR304WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43310">
        <property type="entry name" value="SULFATE TRANSPORTER YBAR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01740">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SpoIIaa-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="211"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="306"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="340"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="386"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="415"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="479"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="512"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="552"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="605"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="domain" description="SLC26A/SulP transporter" evidence="10">
        <location>
            <begin position="213"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="domain" description="STAS" evidence="11">
        <location>
            <begin position="673"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="19"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="67"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="19"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR24386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1010" mass="112552" checksum="1B3CBB07C57963E5" modified="2009-07-28" version="1">MGFSRPRNKHSLSEAISVSLGLQSQPGSETDTLSVPRGNPFSNGADGSPHYYLGRSYVANLSPPPLAIKERFSGDPTSSSRPEQSQSIHQSLSLRRQTAALSNEFDGTFEFEDQLEDLAENGNTAVPDESVMAGSAISPTSSGPEPSVLDNTDVSNLLLPPNTQRDLEYGSIDEFDHQYVSLQSQRAGSNSRAIDPNASMRDKTLFTFRRLGNYVPAVILGLLLNILDALSYGMIIFPITEPLFSHLGPSGLSMFYMSSVVSQLVYSCGFSAFGNAVGSEMIEITPFYHSMAASIMASMPGEKDRVLTTTIVCYAMSSIMTGLVFFLLGRLKLGKIVGFFPRHILIGCIGGVGYFLVITGFEVTTRASKFEYSFEFLSSLLTDPEILGKWGLPALMAVLLVVIQRACKNSLVLPTFYIAALLLFHFIVALIPSLSLHSLRDSGWVFPATTTTEHWFDFYKLYNFKLVSWPLVLKQVPTMLALTFFGILHVPINVPALAMSIGLDKLDVDKELIAHGYSNFLSGMIGSVQNYLVYTNSLLFIKAGADDSLAGLMLAAATFGIMVIGPVIVAYIPVCIVGSLIFLLGYELLKEALVDTWGKLNNFEYATVVAIVFTMGAFDFVLGIIVGILIACFSFLVDSTKLQTINGEFDGQVARSTVRRDYIQTQFLNKIGEQIHVLKLQNLLFFGTIISIEEKIDKLLELSDKDASKKRIKYLILDFKNINADNIDYSAAEGFNRIKRFTFSKRIHLIISSIKTTDRIYRAFNKVGTLDGVELFDDLNSALEWCENEFLRQFRDLRNKTKLKAVRKQERQLSKGALPFNTPRNNHFVSVAQKLFTDEQSMPHLKTHYKEKTPVLHLLLSALQRYRPEIMSPEKPVKETEEKMWSELGKFFFKRRLAAQSLLPHKNNIFLVVESGMVKVTYNLRQGQLYEILSGKTCYGRITAPNSSEVPISSVEIKMETDCALWILDEANLAKLKKENLALYTELLLVTLCMNQDRLKELLGHCLVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJD2</accession>
    <name>C5DJD2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0F15488p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0F15488g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554858.1">
        <property type="nucleotide sequence ID" value="XM_002554812.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJD2"/>
    <dbReference type="FunCoup" id="C5DJD2">
        <property type="interactions" value="17"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJD2"/>
    <dbReference type="GeneID" id="8293089"/>
    <dbReference type="KEGG" id="lth:KLTH0F15488g"/>
    <dbReference type="eggNOG" id="ENOG502QQUZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_324667_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJD2"/>
    <dbReference type="OMA" id="CFNMINS"/>
    <dbReference type="OrthoDB" id="66409at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051666">
        <property type="term" value="P:actin cortical patch localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000282">
        <property type="term" value="P:cellular bud site selection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052809">
        <property type="entry name" value="Actin_polarity_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012860">
        <property type="entry name" value="Afi1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037516">
        <property type="entry name" value="Tripartite_DENN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28245">
        <property type="entry name" value="ARF3-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28245:SF1">
        <property type="entry name" value="ARF3-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07792">
        <property type="entry name" value="Afi1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08616">
        <property type="entry name" value="SPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50211">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDENN" evidence="1">
        <location>
            <begin position="31"/>
            <end position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="888" mass="101676" checksum="3652BB9FDE7281B8" modified="2009-07-28" version="1">MFTHSHAIRSGLGGRLRQSHDEHISYRYNAQYIISAEFDNKVGPVIKHQHPKPLAGFKSSTSRSASVNLASLMIPNSAESRPDVADFTVFILYKDKYTRNYHVFPIADSKLPQNDARSPTILEEDEPQSGLAGEASTNHNADTKEPPLFFLSVVNALHDKSNDRGATIKSIALGTTMQNFIIFKPLLAMTLDLYMRSNDDPRLLIDCFNMINSLDLSFAKRTLANASLQNLLNSISDENLIAEIFNPIKQNLQNILHLNTLPKADVFGNKISFKRHLVEYQFTKFKPTLLPPALSRISLQIDLINFDPVKVNINYNDHVLKFLLKFIPQLDELPRSRFSWRLFVNSTKLPKDTLCQFILSLSNFIKHFDFHYFENAQVIIFPYMDISFLDALREQLVLQNGRRMFTIVGVSNPIFEYQKDVWDFYYDMDAGSLQTRVSSMQSVASLQSISQETAKSSKKMKNIFQKRASYAMPLTVPSKKCGLTWKMINYLVNERHDNETVLNVFKRINILQLLSLLKDIDNRPQAEAELSLKDDYVVTYRDFIIFPEFFEYGTLKLIRCFAQLEQDLNYILFDGKKSGGGNNYNVLESIFASLKQINKFMASSTANTDKFFNIGLNFPLTAIQREESLTRKDFLDVDLEKYFSELKMRNCMEMLDSHHKGVVDYFAMKRFQRLLFLPLLIDPEIEYCSDYSSRFGLADAAHEDSGSNIKRILMSEISLSPMSSIFNDDSLFRDGNFEDLKSRTLSSKSGSIGRDSFRDRSELIVPKIRSACLAIVHRTQKHAVGRLLLEYKLNPMFQSIFASLREETISKRKSDIEKRDSLRKIQVSAHEVNMNVSPSRRNLLNDLNAISQQQDDIKSKMASEGKPARASILSTLNRLTSGLDEMEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJD8</accession>
    <name>C5DJD8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="15">KLTH0F15620p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0F15620g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the snRNP Sm proteins family. SmF/LSm6 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554864.1">
        <property type="nucleotide sequence ID" value="XM_002554818.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJD8"/>
    <dbReference type="FunCoup" id="C5DJD8">
        <property type="interactions" value="902"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJD8"/>
    <dbReference type="GeneID" id="8293095"/>
    <dbReference type="KEGG" id="lth:KLTH0F15620g"/>
    <dbReference type="eggNOG" id="KOG1783">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076902_7_1_1"/>
    <dbReference type="InParanoid" id="C5DJD8"/>
    <dbReference type="OMA" id="MYISEQK"/>
    <dbReference type="OrthoDB" id="268799at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005732">
        <property type="term" value="C:sno(s)RNA-containing ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005688">
        <property type="term" value="C:U6 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000037">
        <property type="entry name" value="U6 snRNA-associated Sm-like protein LSm6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016487">
        <property type="entry name" value="Lsm6/sSmF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11021">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN F SNRNP-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11021:SF1">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 13" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="10 13" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="11 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 13" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="9 13" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="8 13" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Sm" evidence="14">
        <location>
            <begin position="11"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR006609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR24427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="84" mass="9085" checksum="A441AD86F80F2366" modified="2009-07-28" version="1">MSSSGESTTTASAQFLSNIIGQSVVVKLHNGMLYQGVLESIDGFMNIALNQATEHYETASNGLLHRYDGDVFVRGTQVLYISEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJL5</accession>
    <name>C5DJL5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0F17424p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0F17424g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0538 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554941.1">
        <property type="nucleotide sequence ID" value="XM_002554895.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJL5"/>
    <dbReference type="FunCoup" id="C5DJL5">
        <property type="interactions" value="74"/>
    </dbReference>
    <dbReference type="GeneID" id="8293176"/>
    <dbReference type="KEGG" id="lth:KLTH0F17424g"/>
    <dbReference type="eggNOG" id="KOG4147">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117792_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJL5"/>
    <dbReference type="OMA" id="YRNVKNH"/>
    <dbReference type="OrthoDB" id="937at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018794">
        <property type="entry name" value="UPF0538"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18444">
        <property type="entry name" value="UPF0538 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18444:SF9">
        <property type="entry name" value="UPF0538 PROTEIN C2ORF76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10209">
        <property type="entry name" value="DUF2340"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="148" mass="16796" checksum="9DF7FACE41AB3085" modified="2009-07-28" version="1">MSTSGNFDVEEPLISSVKPLTDATITVRVIKSFPYRNVKNIVFTGYDLTSKNAQELLADSVAHIKTTGAFRAFRNVDYNTLKIYTHAHGSKTVNLVVNFDHDEDWVLDTTEKGNDKKLVDYGIGNETEISLFNDQDYLQFKANPEEKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJN1</accession>
    <name>C5DJN1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosome production factor 2 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Ribosome biogenesis protein RPF2 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0F17776g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Nucleus</location>
            <location evidence="1 4">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the RPF2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554957.1">
        <property type="nucleotide sequence ID" value="XM_002554911.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJN1"/>
    <dbReference type="FunCoup" id="C5DJN1">
        <property type="interactions" value="1099"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJN1"/>
    <dbReference type="GeneID" id="8293192"/>
    <dbReference type="KEGG" id="lth:KLTH0F17776g"/>
    <dbReference type="eggNOG" id="KOG3031">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049783_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJN1"/>
    <dbReference type="OMA" id="VGLKPMF"/>
    <dbReference type="OrthoDB" id="407658at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007109">
        <property type="entry name" value="Brix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039770">
        <property type="entry name" value="Rpf2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12728">
        <property type="entry name" value="BRIX DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12728:SF0">
        <property type="entry name" value="RIBOSOME PRODUCTION FACTOR 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04427">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00879">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50833">
        <property type="entry name" value="BRIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Brix" evidence="6">
        <location>
            <begin position="28"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="317"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR24520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="345" mass="39569" checksum="C480BD99A62A8E12" modified="2009-07-28" version="1">MIRTVKPKNARAKRALDKKEPKLVENTKQALFVTGKSSTKVLHDAMVDLSALKKPDIKRFNKKNEVRPFEDAEPLEFFSEKNDTSLMVLGTSSKKRKNNLTFVRTFGYKIYDMIELQIADNYKFLSDFKKQTFAVGLKPMFSFQGSAFDTHPVYKHVKSLFLDFFRGTTTDLHDVAGLQHVISLTIQGDFEDGESLPNVLFRVYKLKTYKSEQGGRKLPRVELEEVGPRFDFKIGRIHSPSPELEKEALKRPKQLEVKTVKNIQLDSMGDKIGTIHMNKQDLGKLQTRKMKGLKSRFDQTSVDSDTENYLNDEEQELQGAEESYGEDFVTANDIDVEPPAKRMKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJQ4</accession>
    <name>C5DJQ4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pyrroline-5-carboxylate reductase</fullName>
            <ecNumber evidence="5">1.5.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0F18282g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-proline + NADP(+) = (S)-1-pyrroline-5-carboxylate + NADPH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:14109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17388"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="EC" id="1.5.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Amino-acid biosynthesis; L-proline biosynthesis; L-proline from L-glutamate 5-semialdehyde: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the pyrroline-5-carboxylate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.2" evidence="5"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554980.1">
        <property type="nucleotide sequence ID" value="XM_002554934.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJQ4"/>
    <dbReference type="FunCoup" id="C5DJQ4">
        <property type="interactions" value="488"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJQ4"/>
    <dbReference type="GeneID" id="8293215"/>
    <dbReference type="KEGG" id="lth:KLTH0F18282g"/>
    <dbReference type="eggNOG" id="KOG3124">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042344_1_2_1"/>
    <dbReference type="InParanoid" id="C5DJQ4"/>
    <dbReference type="OMA" id="VWAVKPQ"/>
    <dbReference type="OrthoDB" id="10263291at2759"/>
    <dbReference type="UniPathway" id="UPA00098">
        <property type="reaction ID" value="UER00361"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004735">
        <property type="term" value="F:pyrroline-5-carboxylate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3730.10:FF:000001">
        <property type="entry name" value="Pyrroline-5-carboxylate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3730.10">
        <property type="entry name" value="ProC C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01925">
        <property type="entry name" value="P5C_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028939">
        <property type="entry name" value="P5C_Rdtase_cat_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053790">
        <property type="entry name" value="P5CR-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029036">
        <property type="entry name" value="P5CR_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000304">
        <property type="entry name" value="Pyrroline-COOH_reductase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00112">
        <property type="entry name" value="proC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11645">
        <property type="entry name" value="PYRROLINE-5-CARBOXYLATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11645:SF0">
        <property type="entry name" value="PYRROLINE-5-CARBOXYLATE REDUCTASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03807">
        <property type="entry name" value="F420_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14748">
        <property type="entry name" value="P5CR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000193">
        <property type="entry name" value="Pyrrol-5-carb_rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00521">
        <property type="entry name" value="P5CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2 4 5" id="KW-0521">NADP</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0641">Proline biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyrroline-5-carboxylate reductase catalytic N-terminal" evidence="6">
        <location>
            <begin position="9"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Pyrroline-5-carboxylate reductase dimerisation" evidence="7">
        <location>
            <begin position="178"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="12"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>NADPH</name>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000193-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR24543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="285" mass="30269" checksum="C2D3D9E9959B6B58" modified="2009-07-28" version="1">MSDSARYTLTILGCGVMGQALLSAIYNAPEASAETKKLYPSKIITCNHDKDSTSQVVKLLEGFGESPNGIEVECTYNDNERAVDEASVVLLSTKPFLAQEVLSAVPVKKKLLISLVAGWTIGQLGEYSQYVTRIMTNTPAKFGYGMAVLSHSDSVSEENKALVRELVGRVGSCIELPEKNMDAATALVGSGPAFALLVLESFMESGLKLGIPLKESKLCAMKVLEGTIKMVEKTGEHPSVLKHQVCTPGGTTIAGLCVMEENGVRSGIIKGVEEAAAVASRLGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DK06</accession>
    <name>C5DK06_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0F00814p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0F00814g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554244.1">
        <property type="nucleotide sequence ID" value="XM_002554198.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DK06"/>
    <dbReference type="FunCoup" id="C5DK06">
        <property type="interactions" value="813"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DK06"/>
    <dbReference type="GeneID" id="8292435"/>
    <dbReference type="KEGG" id="lth:KLTH0F00814g"/>
    <dbReference type="eggNOG" id="KOG2201">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011154_3_0_1"/>
    <dbReference type="InParanoid" id="C5DK06"/>
    <dbReference type="OMA" id="FKNPDIC"/>
    <dbReference type="OrthoDB" id="498611at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004594">
        <property type="term" value="F:pantothenate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd24123">
        <property type="entry name" value="ASKHA_NBD_PanK-II_Pank4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000115">
        <property type="entry name" value="Pantothenate kinase PanK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.510">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004567">
        <property type="entry name" value="Type_II_PanK"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00555">
        <property type="entry name" value="panK_eukar"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12280:SF20">
        <property type="entry name" value="4'-PHOSPHOPANTETHEINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12280">
        <property type="entry name" value="PANTOTHENATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03630">
        <property type="entry name" value="Fumble"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR23807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="356" mass="39427" checksum="5E723CA83258C5C8" modified="2009-07-28" version="1">MGQNIEFQENQWNSTGLLTIAVDIGGSLAKLVFNPRNCRTLIFETVETEKITEFMQLIHRIIKEYNSGDQATTRIIATGGGAYKFNDLLRSEFPRVIEIARLDEMECLIKGLDFLIHEVPEEVFTYNDLDGEHVVVPEHAKVYPYMLVNIGSGVSISKVDSPTKFTRVGGSSLGGGTLWGLLSLITGARTYDEMLDWAHSGDNTNVDMLVGDIYGTDYGKIGLKSTHIASSFGKVFQRGTHAATADGVLSERPSQTSTEEIETRSRKFSNSDISKSLLYAISNNIGQIAYLQAKVHNVPNIYFGGSYIRGHLMTMNTLSYAINFWSNGKKQAFFLKHEGYLGAMGALLTFQDRSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5DK08</accession>
    <name>C5DK08_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0F00858p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0F00858g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RPAP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554246.1">
        <property type="nucleotide sequence ID" value="XM_002554200.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DK08"/>
    <dbReference type="FunCoup" id="C5DK08">
        <property type="interactions" value="200"/>
    </dbReference>
    <dbReference type="GeneID" id="8292437"/>
    <dbReference type="KEGG" id="lth:KLTH0F00858g"/>
    <dbReference type="eggNOG" id="KOG1894">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031074_0_0_1"/>
    <dbReference type="InParanoid" id="C5DK08"/>
    <dbReference type="OMA" id="DQESPYY"/>
    <dbReference type="OrthoDB" id="348201at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039913">
        <property type="entry name" value="RPAP1/Rba50"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013929">
        <property type="entry name" value="RPAP1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013930">
        <property type="entry name" value="RPAP1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21483">
        <property type="entry name" value="RNA POLYMERASE II-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21483:SF18">
        <property type="entry name" value="RNA POLYMERASE II-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08620">
        <property type="entry name" value="RPAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08621">
        <property type="entry name" value="RPAP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RPAP1 N-terminal" evidence="4">
        <location>
            <begin position="79"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="RPAP1 C-terminal" evidence="3">
        <location>
            <begin position="297"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="40"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="55"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="77"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR23809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="450" mass="51047" checksum="68875A89D4B8DABA" modified="2009-07-28" version="1">MDLLGDIVEKDVSEAPALHEAPPTGFPALYQPEKVSSWKVRLQQKNKQKRTSQTSKRERDTPAKRAVEEPVIPPETEAQRIHQENVTRMRSMTPEQLEQERRELLESLDPAVLHGLLKRAARREEPASLQESMKTVKAPLFAEVEGAPGTWVGGSREFSDLPHLDDAAVDKALGIPETKAKHVKFQDQDDESRAEFPGDEEECFPEPLPDDDMAPQEYQFVQQMDHMNNEDLLKDVHFIRPNTVDNDFEALDINDPSFDEKLHKKYFPDLPKESDKMAWMKPVSSAKPTGLIEDVTQCRFDFQGSLVPPDREIKSTQDGLHHHSEEPELAGYTIVELQHLSRSTFPAQRCIAIQTLGRILYKLGKQSYNQLIPEVDAGTYEEMGGAEAILNHIYGMFWDLCKVSMVIESLTDAADENSTKNLSVRNYAIDALWLWKEGGGDFRTGKNENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DK14</accession>
    <name>C5DK14_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0F00990p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0F00990g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554252.1">
        <property type="nucleotide sequence ID" value="XM_002554206.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DK14"/>
    <dbReference type="FunCoup" id="C5DK14">
        <property type="interactions" value="267"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DK14"/>
    <dbReference type="GeneID" id="8292443"/>
    <dbReference type="KEGG" id="lth:KLTH0F00990g"/>
    <dbReference type="eggNOG" id="ENOG502QU5T">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020222_0_0_1"/>
    <dbReference type="InParanoid" id="C5DK14"/>
    <dbReference type="OMA" id="HFWIIPG"/>
    <dbReference type="OrthoDB" id="2595934at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001080">
        <property type="term" value="P:nitrogen catabolite activation of transcription from RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050797">
        <property type="entry name" value="Carb_Metab_Trans_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31668">
        <property type="entry name" value="GLUCOSE TRANSPORT TRANSCRIPTION REGULATOR RGT1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31668:SF9">
        <property type="entry name" value="URACIL CATABOLISM PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="9">
        <location>
            <begin position="56"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="715"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="130"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="29"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="722"/>
            <end position="741"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR23815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="785" mass="89520" checksum="C82321030EF31561" modified="2009-07-28" version="1">MTEPKNFHLPTDHEPTIHLNTDGNYVKKVRQEDSRSYNRSETEAARKPRKKRKTYSCEVCRKFKTRCDFEPLVGRCHRCSVLKIQCSLTKEREEEILAAIETSSSVALPTSGVSSSEVSSKDSLVSHPVATTLNNRLNKLESNLNRLNSKLDLLVLLAQGSNSAAANSVSNLAGDRKAGSAVGIADLSTNRGDLLGSKPLHNRLDDSDDQLTDEAVSAFKDFSPPPPRPPQRLDLNGLKLQEPPLKLISDIDERLFPTKVESERDRIAKTQRPFVVARVNFLKFFDQHEQLCLDLSRDFLVKSHFWIIPGGIKEIDRSYVEKHLFISSVFAIIAMGFDENNKYEEEQEIMYPLVERLLTNTLTMFEKLTDHDIEAILYCSMFNVSRKSKRHRQMKLNSLVLTNFAISSLLNIIDFHKIKERVLSDKYDSLDLYHLRILNSLTACRLQYSIGYGNFMMSDDLLKDFNNLTARFPQATFGDDIKISEINLSEIVNNIFLNFKTYFKEFTISFKNCLHNNQFALGSQENLNNGVGSSNDTLAFPELEYWLKNWDELLSKDGGGVLLFAFNYYYIMICRSFLTEFYEEYHSDLHYFKCILKTMKNYCFQLLDGFLKLPSSLIKGAPVITVHQLVYACLTLCDFLHFFDASERQQILNLCTKIYWHLHTIGEKLNEATENVGKIINSLIDTGKKKVHVGSDPFSLTEKPKHARKAHSILKKHTVSDSQNSPHSVESSSSHNSGIESATGAGFNMPDVGQFNSFEDFFQDFFENLKPTTQNIFSSMNQTPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKA2</accession>
    <name>C5DKA2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein mL46</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0F02970g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mL46 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554340.1">
        <property type="nucleotide sequence ID" value="XM_002554294.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKA2"/>
    <dbReference type="FunCoup" id="C5DKA2">
        <property type="interactions" value="513"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKA2"/>
    <dbReference type="GeneID" id="8292532"/>
    <dbReference type="KEGG" id="lth:KLTH0F02970g"/>
    <dbReference type="eggNOG" id="KOG4548">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040204_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKA2"/>
    <dbReference type="OMA" id="HPFENAF"/>
    <dbReference type="OrthoDB" id="414075at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04661">
        <property type="entry name" value="NUDIX_MRP_L46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040008">
        <property type="entry name" value="Ribosomal_mL46"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021757">
        <property type="entry name" value="Ribosomal_mL46_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033650">
        <property type="entry name" value="Ribosomal_mL46_NUDIX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13124">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L46, MITOCHONDRIAL PRECURSOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13124:SF12">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11788">
        <property type="entry name" value="MRP-L46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Large ribosomal subunit protein mL46 N-terminal" evidence="9">
        <location>
            <begin position="40"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="107"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="133"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF11788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR23903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="294" mass="33170" checksum="413E0E8DB6F7E1A0" modified="2009-07-28" version="1">MRLEEAEVFVNGLLQQHKGGSMTFQQVALRGMATASKPRAIKAGLILSRIPIVTQEATTLEKQYYEYQSELERRLMWTFPQYFYFRKGTLSEKRFLAAQRGPVSRQPGVWFPKGVPDVRHNRERRTKQEVTLPKSSESESLSGANNEDDVSRPVVPNSRITDADRENDTTSLERQLSSTLYLIVKNAAGKWAFPSFSVPLDSEAKKPLHITAEAGLRELGGPNINTWTVSNTPAGVLGDAQATEFMIKSHIVSGRFVLQPQKQYDAFAWLTKSEIQQRVEEAYFKELSCILADN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKE6</accession>
    <name>C5DKE6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0F04048p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0F04048g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554384.1">
        <property type="nucleotide sequence ID" value="XM_002554338.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKE6"/>
    <dbReference type="GeneID" id="8292579"/>
    <dbReference type="KEGG" id="lth:KLTH0F04048g"/>
    <dbReference type="eggNOG" id="KOG1286">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007946_12_1_1"/>
    <dbReference type="InParanoid" id="C5DKE6"/>
    <dbReference type="OMA" id="QYWTDAN"/>
    <dbReference type="OrthoDB" id="3900342at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000001">
        <property type="entry name" value="Amino acid permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF36">
        <property type="entry name" value="PROLINE-SPECIFIC PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="105"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="186"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="219"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="309"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="411"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="440"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="482"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="517"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="7">
        <location>
            <begin position="75"/>
            <end position="543"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR23947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="583" mass="64201" checksum="0734C5830D0E1574" modified="2009-07-28" version="1">MVKTPQMQELPLSPYASKVAIDNSHSRPNYGHSADSWNNEKSAYQVDQIIDVEDASMELIEGQEHQVARKLKSRHIQLIALGGAIGTGLFIGSGGALSVCGPAPLLISYMIMSFFVWCIMNFLTEMVCMMPISGETSMFAMTGTYLNKPLSFMCGINLFYAMSMIAPSEITATAILIQYWTDANSAIFISIFIVVTVSLTMLPVHFFGESEFWVSIIKLFCITGLVILGIVIFFGGAPNQDKVLGFHYWKHPGAFNPHLVPGNTGKFLACWTAIIKSGFSYVLVPEVVVSCAAEAKDPRRNMPRVAQRFVYRLALFYVCGSLTIGIMVGYDNSRLLNAIAAGESNAAASPFVIGIQEVGIRVLPHIINACILTSAYSCGTSVLYGASRVLHSMAVNGAVPKIFATTNRFGTPYYSTAAASVFCLLAYLNCSDSSSVVFTWLSNIATISGFVDWMLVCVVYLRFRKVIEHANLTDKMPFRKRFMKPLAYLSCGFFAILSLTNGYAVFVADNWNVSDFFACYTTLGLAAVLYIGAAIYYKTIKLRDLDEVAAEIIPKIAMADEEEKGEVPIVPKNWLEKVWYFLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKH8</accession>
    <name>C5DKH8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0F04774p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0F04774g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554416.1">
        <property type="nucleotide sequence ID" value="XM_002554370.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKH8"/>
    <dbReference type="FunCoup" id="C5DKH8">
        <property type="interactions" value="127"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKH8"/>
    <dbReference type="GeneID" id="8292611"/>
    <dbReference type="KEGG" id="lth:KLTH0F04774g"/>
    <dbReference type="eggNOG" id="ENOG502S1AY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_141769_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKH8"/>
    <dbReference type="OMA" id="QNHNGHM"/>
    <dbReference type="OrthoDB" id="1696305at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007741">
        <property type="entry name" value="Ribosomal_mL43/mS25/NADH_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040049">
        <property type="entry name" value="Ribosomal_mS25/mL61"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13274">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOMAL PROTEIN S25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13274:SF2">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05047">
        <property type="entry name" value="L51_S25_CI-B8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00916">
        <property type="entry name" value="L51_S25_CI-B8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein/NADH dehydrogenase" evidence="5">
        <location>
            <begin position="40"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR23979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="131" mass="15163" checksum="B25539C2569093A4" modified="2009-07-28" version="1">MSRVARQLKFLNKISSSTLEPQVVLNSQKYAGLNLTFQYQNHNGHMGARKFWHEYMPTLQFYNPGLQFQVTRVKNEDKNNAGVPCVLQTIGKDGSVLETLDMRDKQHSEIMQEFLEKADYEKVPESDIVRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKI1</accession>
    <name>C5DKI1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">cAMP-dependent protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0F04840g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.11"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.11" evidence="2"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554419.1">
        <property type="nucleotide sequence ID" value="XM_002554373.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKI1"/>
    <dbReference type="FunCoup" id="C5DKI1">
        <property type="interactions" value="865"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKI1"/>
    <dbReference type="GeneID" id="8292614"/>
    <dbReference type="KEGG" id="lth:KLTH0F04840g"/>
    <dbReference type="eggNOG" id="KOG0616">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_63_5_1"/>
    <dbReference type="InParanoid" id="C5DKI1"/>
    <dbReference type="OMA" id="YPETDEQ"/>
    <dbReference type="OrthoDB" id="63267at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004691">
        <property type="term" value="F:cAMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010607">
        <property type="term" value="P:negative regulation of cytoplasmic mRNA processing body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010737">
        <property type="term" value="P:protein kinase A signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05580">
        <property type="entry name" value="STKc_PKA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000005">
        <property type="entry name" value="cAMP-dependent protein kinase catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000005">
        <property type="entry name" value="cAMP-dependent protein kinase catalytic subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353:SF73">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE TYPE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="71"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="14">
        <location>
            <begin position="326"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="344"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="26"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="52"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="371"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR23982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="381" mass="43757" checksum="2336F34C64CB5D2E" modified="2009-07-28" version="1">MSQSEQRMRETLQELPSGLLGSGSVPETQQQSLPETQQQSSLGQQAVEAKNAQKTELQGRQTSGKYTLNDFHILRTLGTGSFGRVHLVRSNHNGRFYAMKVLKKRTIVKLKQVEHTNDERRMLSIVSHPFIIRMWGTFQDAEQVFMIMDYIEGGELFSLLRKSQRFPNPVAKFYAAEVCLALEYLHAHEIIYRDLKPENVLLDKNGHVKITDFGFAKYVPDVTFTLCGTPDYIAPEVVSTKPYNKSVDWWSFGILIFEMLAGYTPFYDSNTMKTYENILNAQLQFPPFFHADVQDLLSQLITRDLSKRLGNLQNGSEDVKNHPWFSEVVWEKLLCRNIETPYEPPVHAGQGDTSQYDRYPEEENSYGAEGDDPHHDLFAEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKM8</accession>
    <name>C5DKM8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0F05984p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0F05984g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the carbon-nitrogen hydrolase superfamily. NIT1/NIT2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554466.1">
        <property type="nucleotide sequence ID" value="XM_002554420.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKM8"/>
    <dbReference type="FunCoup" id="C5DKM8">
        <property type="interactions" value="101"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKM8"/>
    <dbReference type="GeneID" id="8292664"/>
    <dbReference type="KEGG" id="lth:KLTH0F05984g"/>
    <dbReference type="eggNOG" id="KOG0807">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030130_1_2_1"/>
    <dbReference type="InParanoid" id="C5DKM8"/>
    <dbReference type="OMA" id="MRVAVCQ"/>
    <dbReference type="OrthoDB" id="10250282at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07572">
        <property type="entry name" value="nit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.110.10:FF:000024">
        <property type="entry name" value="Deaminated glutathione amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045254">
        <property type="entry name" value="Nit1/2_C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23088:SF27">
        <property type="entry name" value="DEAMINATED GLUTATHIONE AMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23088">
        <property type="entry name" value="NITRILASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="5">
        <location>
            <begin position="54"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR24029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="352" mass="39388" checksum="7C9EA3A26FE91270" modified="2009-07-28" version="1">MLKPTFKFLKTEQWTRCLFKSQKRLAPAWDLLSAPKYNRRFFYSSSSRCQESMNRIAIGQMCSSSSLDQNLGVVKTLIDQALASDAKVIFFPEAADYISRSASHSLELAEKSPAFVEGLQKAIPELCSKHQKRIDVSIGVHLPATSSDKSQHDTRLRNCLLYITHEGKIRQEYQKLHLFDVEVPNGPILKESQAVQPGRAIPDVLDTPVGKLGSAICFDVRFPELALKLRSKGAEILCFPSAFTMKTGEAHWQLLACARALDTQCYVIMPGQQGEHEVGIDPSKPGESPLKRVSWGHSMVVGPWGKVVAAADPANNEPQLLLADLDLDAQKKIRRDMPLWEQRRRDVFGDFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKM9</accession>
    <name>C5DKM9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRM61</fullName>
            <ecNumber evidence="2 9">2.1.1.220</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0F06006g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalytic subunit of tRNA (adenine-N(1)-)-methyltransferase, which catalyzes the formation of N(1)-methyladenine at position 58 (m1A58) in initiator methionyl-tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>adenosine(58) in tRNA + S-adenosyl-L-methionine = N(1)-methyladenosine(58) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43152"/>
            <dbReference type="Rhea" id="RHEA-COMP:10365"/>
            <dbReference type="Rhea" id="RHEA-COMP:10366"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74491"/>
            <dbReference type="EC" id="2.1.1.220"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM61 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.220" evidence="2 9"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554467.1">
        <property type="nucleotide sequence ID" value="XM_002554421.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKM9"/>
    <dbReference type="FunCoup" id="C5DKM9">
        <property type="interactions" value="656"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKM9"/>
    <dbReference type="GeneID" id="8292665"/>
    <dbReference type="KEGG" id="lth:KLTH0F06006g"/>
    <dbReference type="eggNOG" id="KOG2915">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025402_4_0_1"/>
    <dbReference type="InParanoid" id="C5DKM9"/>
    <dbReference type="OMA" id="RPDHRMI"/>
    <dbReference type="OrthoDB" id="1925287at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031515">
        <property type="term" value="C:tRNA (m1A) methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160107">
        <property type="term" value="F:tRNA (adenine(58)-N1)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.330.20:FF:000002">
        <property type="entry name" value="tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049470">
        <property type="entry name" value="TRM61_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014816">
        <property type="entry name" value="tRNA_MeTrfase_Gcd14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12133">
        <property type="entry name" value="TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12133:SF2">
        <property type="entry name" value="TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASE CATALYTIC SUBUNIT TRMT61A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08704">
        <property type="entry name" value="GCD14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14801">
        <property type="entry name" value="TrmI-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017269">
        <property type="entry name" value="GCD14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51620">
        <property type="entry name" value="SAM_TRM61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 9" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRM61 C-terminal" evidence="12">
        <location>
            <begin position="69"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="305"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="377"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR017269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR017269-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF08704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR24030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="400" mass="45837" checksum="730729CAF3688AC9" modified="2009-07-28" version="1">MSHFSEYKDLIQVGDLALVWISRDNIKPVTITAGETFNTRYGSFPHDDMIGKPYGSQIAVRTKGSNRFGFIHVLQPTPELWTISLPHRTQIVYTPDSSYIMQRLQCSPHSRVIEAGTGSGSFSHAFARSAGHLFSYEFHEVRYEQAKKEFEEHGLFRDGNVTITHRDVCHDGFTIKNTDKTTFFDGKSAEEAQQPACIGADVIFLDLPAPWDAIPNLDCVISQNSKTSMCCFSPCIEQVDKTLEALEKHGWTEVEMVEIQGRQYESRRQMVRHLDDALQRLRDVRERKQVGVERRKRMFNKIISDQEEPYEERPETPKTQFNPFGKGSRVKEGDSNYSWKEVSKVESEIKSHTSYLTFASKIVHKARNEELVQSIIEEHKDVTPESTLPGKKDKKQSVNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>C5DL03</accession>
    <name>C5DL03_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0F08910p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0F08910g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554591.1">
        <property type="nucleotide sequence ID" value="XM_002554545.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DL03"/>
    <dbReference type="FunCoup" id="C5DL03">
        <property type="interactions" value="93"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DL03"/>
    <dbReference type="GeneID" id="8292797"/>
    <dbReference type="KEGG" id="lth:KLTH0F08910g"/>
    <dbReference type="eggNOG" id="ENOG502S4AD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_138722_0_0_1"/>
    <dbReference type="InParanoid" id="C5DL03"/>
    <dbReference type="OMA" id="FDCRDVY"/>
    <dbReference type="OrthoDB" id="10265275at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR24154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="170" mass="19042" checksum="E821580525345924" modified="2009-07-28" version="1">MDDELASKDLSAHVVVALEDPSHIHYKDLWLESSTGAEQCVLELFSFGKVKDAGAELRAMLTTAMWQKLQRLTMLTLSCQERVLSYERIRDECALGACGEAEQLVMSLGSLAEFSIDQVKQLVVVQKCRDCRDVYNGERALIVVGSPVASGQEILHDLQRWQRKLGNDML</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>C5DL05</accession>
    <name>C5DL05_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">mitochondrial processing peptidase</fullName>
            <ecNumber evidence="5">3.4.24.64</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Beta-MPP</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">KLTH0F08954g</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalytic subunit of the essential mitochondrial processing protease (MPP), which cleaves the mitochondrial sequence off newly imported precursors proteins. Preferentially, cleaves after an arginine at position P2.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of N-terminal transit peptides from precursor proteins imported into the mitochondrion, typically with Arg in position P2.</text>
            <dbReference type="EC" id="3.4.24.64"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the peptidase M16 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.64" evidence="5"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554593.1">
        <property type="nucleotide sequence ID" value="XM_002554547.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DL05"/>
    <dbReference type="FunCoup" id="C5DL05">
        <property type="interactions" value="932"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DL05"/>
    <dbReference type="MEROPS" id="M16.003"/>
    <dbReference type="GeneID" id="8292799"/>
    <dbReference type="KEGG" id="lth:KLTH0F08954g"/>
    <dbReference type="eggNOG" id="KOG0960">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009902_4_0_1"/>
    <dbReference type="InParanoid" id="C5DL05"/>
    <dbReference type="OMA" id="IDVVCDM"/>
    <dbReference type="OrthoDB" id="10251424at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006627">
        <property type="term" value="P:protein processing involved in protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000002">
        <property type="entry name" value="Mitochondrial-processing peptidase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000001">
        <property type="entry name" value="Mitochondrial-processing peptidase subunit beta, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050361">
        <property type="entry name" value="MPP/UQCRC_Complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001431">
        <property type="entry name" value="Pept_M16_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851:SF149">
        <property type="entry name" value="GH01077P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851">
        <property type="entry name" value="METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00143">
        <property type="entry name" value="INSULINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="16">
        <location>
            <begin position="33"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="17">
        <location>
            <begin position="186"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAR24156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="458" mass="50509" checksum="34628848B6DFC9B7" modified="2009-07-28" version="1">MLRNLLFGTRFSRPLATAASSTRTSVLRNGLTVATEHIPNTSSATVGIFVDAGSRAENTRNNGTAHFLEHLAFKGTKNRTQVGIELEIENIGSHLNAYTSRENTVYYAKTLTQNIPNAVDVLSDILTRSVLDARAIERERDVIIRESEEVDKMYDEVVFDHLHAITYKDQPLGRTILGPIENIKTIQRRDLQDYISKNYKGDRMVLAGAGAVDHEKLVEYADKYFGHIPKSESPVPLGSPRGPLPVFYGNEMNIQEDTLPTTHIALAVEGVSWSAPDYFTALATQAIVGNWDRALGTGTNSPSPLAVSASNNGTLANSYMSFSTSYADSGLWGMYIVIDSKEHNAKLIIDEVLKDWQRIKSGNISDEEVMRAKSQLKASLLLSLDGSTAIVEDIGRQIVTTGKRLSPEEVFEQVDRITKDDIITWANYRLKDKPVSIVALGNTKTVPALKEIEQGLNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLB8</accession>
    <name>C5DLB8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0F11682p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0F11682g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554706.1">
        <property type="nucleotide sequence ID" value="XM_002554660.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLB8"/>
    <dbReference type="FunCoup" id="C5DLB8">
        <property type="interactions" value="163"/>
    </dbReference>
    <dbReference type="GeneID" id="8292919"/>
    <dbReference type="KEGG" id="lth:KLTH0F11682g"/>
    <dbReference type="eggNOG" id="ENOG502SAQZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_149479_0_0_1"/>
    <dbReference type="InParanoid" id="C5DLB8"/>
    <dbReference type="OrthoDB" id="2107880at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061671">
        <property type="term" value="C:Cbp3p-Cbp6 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034551">
        <property type="term" value="P:mitochondrial respiratory chain complex III assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037653">
        <property type="entry name" value="Cbp6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28250">
        <property type="entry name" value="CYTOCHROME B PRE-MRNA-PROCESSING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28250:SF1">
        <property type="entry name" value="CYTOCHROME B PRE-MRNA-PROCESSING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20180">
        <property type="entry name" value="UQCC2_CBP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR24269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="156" mass="17565" checksum="1821894F3D017F3D" modified="2009-07-28" version="1">MSSAVRGAAVQLVKTIEKLPAERMKHMVSLKESQLERFKPVAGLEAGAHGSKQKPTLQEIKDIISRTSGPLGLQKEALKKVQEAFPQEQYTAEAIQEQTKSLNNILSNKYRDYYAVGDKLLKPQGNPAYYQRLMDEIEGKKKETFWTAMRTVVLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLD5</accession>
    <name>C5DLD5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0F12078p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0F12078g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554723.1">
        <property type="nucleotide sequence ID" value="XM_002554677.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLD5"/>
    <dbReference type="FunCoup" id="C5DLD5">
        <property type="interactions" value="26"/>
    </dbReference>
    <dbReference type="GeneID" id="8292937"/>
    <dbReference type="KEGG" id="lth:KLTH0F12078g"/>
    <dbReference type="eggNOG" id="ENOG502S4TY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_957155_0_0_1"/>
    <dbReference type="InParanoid" id="C5DLD5"/>
    <dbReference type="OMA" id="GTEMLYN"/>
    <dbReference type="OrthoDB" id="4068068at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="134"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="212"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR24286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="298" mass="33916" checksum="6598DD2F4299BA20" modified="2009-07-28" version="1">MPHQLIPEVYKRRRKQLQDDLVNQVSFLGYAVIVLHYLKFGSSTLTLILRLCIQSLLTSPYPSEFQLRRLTFSAQSRTIPGLRRDNGASVPQTINRSGTRIPGTFTPSDQPAIEHETEVETEDISDRLRHNIRWILFHCSFTVNFILIMLSIIWPIDFQAKVGEYRLNEKGLSDVPSPFNNHNGLVGGELRGRIFIQFIGERVSSSNFRENLGLLFLEVLIFICQFGLYLLTCYNLAKPPIDAFTSEDSDAVRENDIEYGGYGGNVLATTVDPMKVFDHILQERYARPFSRPTGSEAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLE6</accession>
    <name>C5DLE6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Acireductone dioxygenase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Acireductone dioxygenase (Fe(2+)-requiring)</fullName>
            <shortName evidence="11">ARD'</shortName>
            <shortName evidence="11">Fe-ARD</shortName>
            <ecNumber evidence="11">1.13.11.54</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Acireductone dioxygenase (Ni(2+)-requiring)</fullName>
            <shortName evidence="11">ARD</shortName>
            <shortName evidence="11">Ni-ARD</shortName>
            <ecNumber evidence="11">1.13.11.53</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ADI1</name>
        <name evidence="12" type="ordered locus">KLTH0F12408g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes 2 different reactions between oxygen and the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) depending upon the metal bound in the active site. Fe-containing acireductone dioxygenase (Fe-ARD) produces formate and 2-keto-4-methylthiobutyrate (KMTB), the alpha-ketoacid precursor of methionine in the methionine recycle pathway. Ni-containing acireductone dioxygenase (Ni-ARD) produces methylthiopropionate, carbon monoxide and formate, and does not lie on the methionine recycle pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + O2 = 3-(methylsulfanyl)propanoate + CO + formate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:14161"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:17245"/>
            <dbReference type="ChEBI" id="CHEBI:49016"/>
            <dbReference type="ChEBI" id="CHEBI:49252"/>
            <dbReference type="EC" id="1.13.11.53"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + O2 = 4-methylsulfanyl-2-oxobutanoate + formate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:24504"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:16723"/>
            <dbReference type="ChEBI" id="CHEBI:49252"/>
            <dbReference type="EC" id="1.13.11.54"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <cofactor evidence="11">
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </cofactor>
        <text evidence="11">Binds either 1 Fe or Ni cation per monomer. Iron-binding promotes an acireductone dioxygenase reaction producing 2-keto-4-methylthiobutyrate, while nickel-binding promotes an acireductone dioxygenase reaction producing 3-(methylsulfanyl)propanoate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="11">Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 5/6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the acireductone dioxygenase (ARD) family.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.54" evidence="11"/>
    <dbReference type="EC" id="1.13.11.53" evidence="11"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554734.1">
        <property type="nucleotide sequence ID" value="XM_002554688.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLE6"/>
    <dbReference type="FunCoup" id="C5DLE6">
        <property type="interactions" value="299"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLE6"/>
    <dbReference type="GeneID" id="8292952"/>
    <dbReference type="KEGG" id="lth:KLTH0F12408g"/>
    <dbReference type="eggNOG" id="KOG2107">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090154_1_1_1"/>
    <dbReference type="InParanoid" id="C5DLE6"/>
    <dbReference type="OMA" id="WYMDESQ"/>
    <dbReference type="OrthoDB" id="1867259at2759"/>
    <dbReference type="UniPathway" id="UPA00904">
        <property type="reaction ID" value="UER00878"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010308">
        <property type="term" value="F:acireductone dioxygenase (Ni2+-requiring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010309">
        <property type="term" value="F:acireductone dioxygenase [iron(II)-requiring] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02232">
        <property type="entry name" value="cupin_ARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000099">
        <property type="entry name" value="1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03154">
        <property type="entry name" value="Salvage_MtnD_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004313">
        <property type="entry name" value="ARD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027496">
        <property type="entry name" value="ARD_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23418">
        <property type="entry name" value="ACIREDUCTONE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23418:SF0">
        <property type="entry name" value="ACIREDUCTONE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03079">
        <property type="entry name" value="ARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 11" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="8 11" id="KW-0408">Iron</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 11" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="3 11" id="KW-0533">Nickel</keyword>
    <keyword evidence="10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="11">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <note>for iron-dependent acireductone dioxygenase activity</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
            <note>for nickel-dependent acireductone dioxygenase activity</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <note>for iron-dependent acireductone dioxygenase activity</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
            <note>for nickel-dependent acireductone dioxygenase activity</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <note>for iron-dependent acireductone dioxygenase activity</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
            <note>for nickel-dependent acireductone dioxygenase activity</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <note>for iron-dependent acireductone dioxygenase activity</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
            <note>for nickel-dependent acireductone dioxygenase activity</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR24297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="176" mass="20450" checksum="A3C7D2489313541D" modified="2009-07-28" version="1">MVEAYYHDNDDSVDFREPHNSGEALSLEQLANIGVIYKHCPTEAEMDKVALERGYKNRDTVRMSSETLGDQLLPKLRTFYTEHLHEDEEIRYIIDGEGYFDVRNEPDDRWVRCKLVPGDLLILPAGIYHRFTLTTDNYVAAVRLFQDEPKWIAHSRPDADGFPIRREYLSSVQASK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLG3</accession>
    <name>C5DLG3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0G00506p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0G00506g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555061.1">
        <property type="nucleotide sequence ID" value="XM_002555015.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLG3"/>
    <dbReference type="FunCoup" id="C5DLG3">
        <property type="interactions" value="54"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLG3"/>
    <dbReference type="GeneID" id="8293319"/>
    <dbReference type="KEGG" id="lth:KLTH0G00506g"/>
    <dbReference type="eggNOG" id="ENOG502RZ15">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_630164_0_0_1"/>
    <dbReference type="InParanoid" id="C5DLG3"/>
    <dbReference type="OrthoDB" id="2507795at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018822">
        <property type="entry name" value="UPF0646"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10336">
        <property type="entry name" value="DUF2420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="311"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="311"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="332"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="372"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="435" mass="48177" checksum="FCFC1E2374BBC9A3" modified="2009-07-28" version="1">MVEASDSPAVVSETEVSLKKAPEVIVGSSRSHAEALSTFEKKQRAMDGPQKSPEGSRHQSPEPNYFSLQSPEPHSEREVTVDNDDSSKINGEERSKLKQGYQSDDDNDSIIDIVSESEVYEAAKQLLSSKPPQIRVNYQGESYLLLGEYDGDNAKEWSSDKIICTDSADLHRGCNVAFGRMRLFFQQVHGGLELLSKELSFEFPDLDLVMEEDNMYNKDITVNDVVSIFKIIKDNSIKKCEPDVSNHLTINVSTKPRFVSRYNSLVEMLNGDATFSNIKVFTNDRSHPVVLDESEQTPNSKDAEVIIMSSEGEEADENEEADDNEADDDETGGEKVNDEEAGDEEPRKIELDGMERDGKESFGVHRDGLTPGNGTSVSNPAVLSEELGQHNAFSPKRGAEKPEHSGIQMAEVDNSESGDSLKRRRDSVSDGDLFV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2010-10-05" modified="2025-06-18" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLJ0</accession>
    <name>IRC6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName>Increased recombination centers protein 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">IRC6</name>
        <name type="ordered locus">KLTH0G01100g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in gross chromosomal rearrangements (GCRs) and telomere healing.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IRC6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555088.1">
        <property type="nucleotide sequence ID" value="XM_002555042.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLJ0"/>
    <dbReference type="SMR" id="C5DLJ0"/>
    <dbReference type="FunCoup" id="C5DLJ0">
        <property type="interactions" value="28"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLJ0"/>
    <dbReference type="GeneID" id="8293346"/>
    <dbReference type="KEGG" id="lth:KLTH0G01100g"/>
    <dbReference type="eggNOG" id="ENOG502S7AE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079666_0_0_1"/>
    <dbReference type="InParanoid" id="C5DLJ0"/>
    <dbReference type="OMA" id="GMESACT"/>
    <dbReference type="OrthoDB" id="10261384at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11960">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034627">
        <property type="entry name" value="Irc6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28043">
        <property type="entry name" value="INCREASED RECOMBINATION CENTERS PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28043:SF1">
        <property type="entry name" value="INCREASED RECOMBINATION CENTERS PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0160">Chromosomal rearrangement</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000399223" description="Increased recombination centers protein 6">
        <location>
            <begin position="1"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="251" mass="28309" checksum="19FEAB8514FBC4A7" modified="2009-07-28" version="1">MTTGLSLDEGKVIEGRPKALRDKVLIVFSNKVFNREEILFEVFGVEDNAGKQVHRGVDWTTKYYTVTIDVYVDSFSNLEDWTNEFCDPDFDELRDVIAGWIIVLPFSGNIEEETKALSRLVNNATSEDFFTAILATELNVGKNERAFLEYNVLAGAVELVHNELDSDGELKEVNGVDRVKEIIDTCDWHPRLLRTAHLESETGMESACTGISLAQIMGSLREARTKYMAMDPGLDREEFANEMANELSKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DM62</accession>
    <name>C5DM62_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribosome-recycling factor, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Ribosome-releasing factor, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0G06270g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Necessary for protein synthesis in mitochondria. Functions as a ribosome recycling factor in mitochondria.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RRF family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555310.1">
        <property type="nucleotide sequence ID" value="XM_002555264.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DM62"/>
    <dbReference type="FunCoup" id="C5DM62">
        <property type="interactions" value="80"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DM62"/>
    <dbReference type="GeneID" id="8293578"/>
    <dbReference type="KEGG" id="lth:KLTH0G06270g"/>
    <dbReference type="eggNOG" id="KOG4759">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085410_0_0_1"/>
    <dbReference type="InParanoid" id="C5DM62"/>
    <dbReference type="OMA" id="PNNDQQL"/>
    <dbReference type="OrthoDB" id="407355at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.20">
        <property type="entry name" value="Ribosome-recycling factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002661">
        <property type="entry name" value="Ribosome_recyc_fac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023584">
        <property type="entry name" value="Ribosome_recyc_fac_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036191">
        <property type="entry name" value="RRF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20982:SF3">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOME RECYCLING FACTOR PSEUDO 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20982">
        <property type="entry name" value="RIBOSOME RECYCLING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01765">
        <property type="entry name" value="RRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55194">
        <property type="entry name" value="Ribosome recycling factor, RRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosome recycling factor" evidence="7">
        <location>
            <begin position="74"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="201"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00033107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR24873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="239" mass="26774" checksum="023F2924A3934D0D" modified="2009-07-28" version="1">MLASVKLVSPLYRVLSQKGVQFRQFQTCYNLLKKASKKSGKGKIDEEEPTEVVDVKQYVLKAKTQFSVTLDLHKKKLSEIRAGSASPSIFDKLSVGKENSKFTDVATTSMKGKNSLIVTVFDPKDTKSVVSSILAAGLNLNPEKIPNNDQQLKISLPPPTTETRKQTCKQLKEVFEEFKNSANKNSLGHIRGDILKQLKNIDKKNDSVKKVIQDLDKLHKEYTNTLQEQLKQAEKNVLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DM64</accession>
    <name>C5DM64_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">V-type proton ATPase subunit G</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0G06314g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">V-ATPase is a heteromultimeric enzyme made up of two complexes: the ATP-hydrolytic V1 complex and the proton translocation V0 complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the V-ATPase G subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555312.1">
        <property type="nucleotide sequence ID" value="XM_002555266.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DM64"/>
    <dbReference type="FunCoup" id="C5DM64">
        <property type="interactions" value="317"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DM64"/>
    <dbReference type="GeneID" id="8293580"/>
    <dbReference type="KEGG" id="lth:KLTH0G06314g"/>
    <dbReference type="eggNOG" id="KOG1772">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125101_2_1_1"/>
    <dbReference type="InParanoid" id="C5DM64"/>
    <dbReference type="OMA" id="ARKYRQD"/>
    <dbReference type="OrthoDB" id="250802at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000221">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.620:FF:000004">
        <property type="entry name" value="V-type proton ATPase subunit G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.620">
        <property type="entry name" value="F1F0 ATP synthase subunit B, membrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.730">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005124">
        <property type="entry name" value="V-ATPase_G"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01147">
        <property type="entry name" value="V_ATP_synt_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12713:SF11">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12713">
        <property type="entry name" value="VACUOLAR ATP SYNTHASE SUBUNIT G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03179">
        <property type="entry name" value="V-ATPase_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="4 5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0813">Transport</keyword>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="10"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR24875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="114" mass="12509" checksum="A39758E1971F5057" modified="2009-07-28" version="1">MSQPNGIATLLKAEKEAHEIVSKARQYRQEKLKQAKSDAATEINAYKQKKEQELKDFEAKNAGGVGGLEKDAEGKVQVEIQEIQKIGKDKKKNVVKLLVDAVTTPVAEVHVNAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>C5DM90</accession>
    <name>C5DM90_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0G06908p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0G06908g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555338.1">
        <property type="nucleotide sequence ID" value="XM_002555292.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DM90"/>
    <dbReference type="GeneID" id="8293607"/>
    <dbReference type="KEGG" id="lth:KLTH0G06908g"/>
    <dbReference type="eggNOG" id="ENOG502S2V7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072832_0_0_1"/>
    <dbReference type="InParanoid" id="C5DM90"/>
    <dbReference type="OMA" id="KIRTYQR"/>
    <dbReference type="OrthoDB" id="4061572at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060963">
        <property type="term" value="P:positive regulation of ribosomal protein gene transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052146">
        <property type="entry name" value="HOT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022210">
        <property type="entry name" value="TF_GCR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37784:SF2">
        <property type="entry name" value="HIGH-OSMOLARITY-INDUCED TRANSCRIPTION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37784">
        <property type="entry name" value="PROTEIN MSN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12550">
        <property type="entry name" value="GCR1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription activator GCR1-like" evidence="1">
        <location>
            <begin position="263"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="361" mass="41752" checksum="245339DF3D93E980" modified="2009-07-28" version="1">MEAPMNLDEVVNSFKNRVDQLSRDWDAIKSSFVGSQDEELKQLKVKFDLIQEQNSVILIDLYQLKKFLNLEDNNYNNVFSTVLDSPAGALHFPTASSERYAENAVPATSGVTYLGSVEQPRIHDTQSALRTQQLYSSASSKIQEKHPVHLHSLWSEIHSPLINDEISYWKKTRSPMSPQLFSHLRQQNPKPTINDENDATEYLGLSKLIGVETPSKNPREKTHGKLQMKPLNSSLLKSGQTPCSSRHLRYAKDAKDISAYVLKMSGNPKTVGDMYYEYEHQLRPQILEFEKRYGKGQISRLPKVRTYQRRRALINEIEKFARREGKSIEEAVQYFEEIRTQNKKTVPWLYNNLNRILQKYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMB8</accession>
    <name>C5DMB8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA-directed RNA polymerase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0G07546g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">DNA-dependent RNA polymerase which catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555366.1">
        <property type="nucleotide sequence ID" value="XM_002555320.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMB8"/>
    <dbReference type="FunCoup" id="C5DMB8">
        <property type="interactions" value="777"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMB8"/>
    <dbReference type="GeneID" id="8293636"/>
    <dbReference type="KEGG" id="lth:KLTH0G07546g"/>
    <dbReference type="eggNOG" id="KOG3297">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073901_1_0_1"/>
    <dbReference type="InParanoid" id="C5DMB8"/>
    <dbReference type="OMA" id="LGPTLWW"/>
    <dbReference type="OrthoDB" id="10256606at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006384">
        <property type="term" value="P:transcription initiation at RNA polymerase III promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04330">
        <property type="entry name" value="RNAP_III_Rpc25_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000221">
        <property type="entry name" value="DNA-directed RNA polymerase III subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.120">
        <property type="entry name" value="RNA polymerase Rpb7-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013238">
        <property type="entry name" value="RNA_pol_III_Rbc25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036898">
        <property type="entry name" value="RNA_pol_Rpb7-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004519">
        <property type="entry name" value="RNAP_E/RPC8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045113">
        <property type="entry name" value="Rpb7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005576">
        <property type="entry name" value="Rpb7-like_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00448">
        <property type="entry name" value="rpoE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II, III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709:SF1">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08292">
        <property type="entry name" value="RNA_pol_Rbc25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03876">
        <property type="entry name" value="SHS2_Rpb7-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88798">
        <property type="entry name" value="N-terminal, heterodimerisation domain of RBP7 (RpoE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="RNA polymerase Rpb7-like N-terminal" evidence="8">
        <location>
            <begin position="8"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase III subunit Rpc25" evidence="9">
        <location>
            <begin position="83"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="160"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="160"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="170"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU369086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR24929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="208" mass="23919" checksum="6C0F1A71A762712F" modified="2009-07-28" version="1">MFILSKISDLVRVPPDQFHRHTKAAIFHQLNNKYANKVIPHLGLCITIYDLLEVREGQLKPGDGAAYINTTFRALVFKPFVGEIITGWISQCTAEGIKVSLLGVFDDIFIPQKMLFEGCYFSPDDNAWVWPMDEETKLYFDVNEKIRFRVEQEVFVDVKPKSPKEREAEEQMEQNEDSEEKPKIEKPPAYALLGSCQTDGMGLVGWWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMG0</accession>
    <name>C5DMG0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0G08602p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0G08602g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MAD2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555408.1">
        <property type="nucleotide sequence ID" value="XM_002555362.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMG0"/>
    <dbReference type="FunCoup" id="C5DMG0">
        <property type="interactions" value="54"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMG0"/>
    <dbReference type="GeneID" id="8293683"/>
    <dbReference type="KEGG" id="lth:KLTH0G08602g"/>
    <dbReference type="eggNOG" id="ENOG502QZWG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1129615_0_0_1"/>
    <dbReference type="InParanoid" id="C5DMG0"/>
    <dbReference type="OMA" id="CFINLIL"/>
    <dbReference type="OrthoDB" id="21254at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016035">
        <property type="term" value="C:zeta DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.900.10">
        <property type="entry name" value="HORMA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003511">
        <property type="entry name" value="HORMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036570">
        <property type="entry name" value="HORMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045091">
        <property type="entry name" value="Mad2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11842">
        <property type="entry name" value="MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11842:SF10">
        <property type="entry name" value="MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02301">
        <property type="entry name" value="HORMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56019">
        <property type="entry name" value="The spindle assembly checkpoint protein mad2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50815">
        <property type="entry name" value="HORMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HORMA" evidence="2">
        <location>
            <begin position="3"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR24971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="243" mass="28253" checksum="98E45A350E76CC8C" modified="2009-07-28" version="1">MFKVVEKWLQIYFKCIINVVLYYRNVYPKESFDWTTYQAFNLPRHIPLNRHPQLQEYVETLVTDVISKLGYIKNFNLRIVNQEDGACIERYVLDFSEFRHEEVGNTTELEIFDEMRSSLNSLIAQLEKLPKIRPGSVTFEIVIDSEGLDLGHRMNRVHTLKERLEQEQDTNWVKCSEGSFTAEPRPKRSQAQDPRIKMIALAGCDSGPLVIYQFMERLLAPTGESLSQVYESSQTSESSVLFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMK8</accession>
    <name>C5DMK8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">KLTH0G09746p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0G09746g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytochrome c oxidase IV family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555456.1">
        <property type="nucleotide sequence ID" value="XM_002555410.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMK8"/>
    <dbReference type="FunCoup" id="C5DMK8">
        <property type="interactions" value="138"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMK8"/>
    <dbReference type="GeneID" id="8293734"/>
    <dbReference type="KEGG" id="lth:KLTH0G09746g"/>
    <dbReference type="eggNOG" id="KOG4075">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070101_2_0_1"/>
    <dbReference type="InParanoid" id="C5DMK8"/>
    <dbReference type="OMA" id="WYISYGA"/>
    <dbReference type="OrthoDB" id="186013at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00922">
        <property type="entry name" value="Cyt_c_Oxidase_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.442.10:FF:000002">
        <property type="entry name" value="Cytochrome c oxidase subunit V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.442.10">
        <property type="entry name" value="Cytochrome c oxidase subunit IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004203">
        <property type="entry name" value="Cyt_c_oxidase_su4_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036639">
        <property type="entry name" value="Cyt_c_oxidase_su4_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10707:SF10">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10707">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02936">
        <property type="entry name" value="COX4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81406">
        <property type="entry name" value="Mitochondrial cytochrome c oxidase subunit IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR25019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="150" mass="16922" checksum="FA4C437BBA648402" modified="2009-07-28" version="1">MLRSSFARVARVTPARFAQTQAISKAAIVDLHSRWEDLPAVEQQELVSKLTERQKLPWNQMTETEKQAAWYISYGEWGPRRPVHVKGDGAYIAKGVAVGLVGSVVLFAIIRQFGGEEPKTMTKEWQLKSDEYLKSKNANPWGGYSQVQSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMQ0</accession>
    <name>C5DMQ0_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Histone H4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0G10692g</name>
        <name evidence="14" type="ordered locus">KLTH0G16236g</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
            <strain evidence="13">CBS 6340</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2009-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Genoscope - CEA"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CBS 6340</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the histone H4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555498.1">
        <property type="nucleotide sequence ID" value="XM_002555452.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMQ0"/>
    <dbReference type="SMR" id="C5DMQ0"/>
    <dbReference type="FunCoup" id="C5DMQ0">
        <property type="interactions" value="1559"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMQ0"/>
    <dbReference type="GeneID" id="8293776"/>
    <dbReference type="KEGG" id="lth:KLTH0G10692g"/>
    <dbReference type="KEGG" id="lth:KLTH0G16236g"/>
    <dbReference type="eggNOG" id="KOG3467">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109117_2_3_1"/>
    <dbReference type="InParanoid" id="C5DMQ0"/>
    <dbReference type="OMA" id="QKEHING"/>
    <dbReference type="OrthoDB" id="4061161at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22912">
        <property type="entry name" value="HFD_H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000007">
        <property type="entry name" value="Histone H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CENP-T/H4_C"/>
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    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001951">
        <property type="entry name" value="Histone_H4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019809">
        <property type="entry name" value="Histone_H4_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10484">
        <property type="entry name" value="HISTONE H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15511">
        <property type="entry name" value="CENP-T_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00623">
        <property type="entry name" value="HISTONEH4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00417">
        <property type="entry name" value="H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00047">
        <property type="entry name" value="HISTONE_H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4 10" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="9 10" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CENP-T/Histone H4 histone fold" evidence="12">
        <location>
            <begin position="36"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF15511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR25061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR25303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="103" mass="11352" checksum="69AE3666FE62DE41" modified="2009-07-28" version="1">MSGRGKGGKGLGKGGAKRHRKILRDNIQGITKPAIRRLARRGGVKRISGLIYEEVRAVLKSFLESVIRDAVTYTEHAKRKTVTSLDVVYALKRQGRTLYGFGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMQ9</accession>
    <name>C5DMQ9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0G10912p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0G10912g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555507.1">
        <property type="nucleotide sequence ID" value="XM_002555461.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMQ9"/>
    <dbReference type="FunCoup" id="C5DMQ9">
        <property type="interactions" value="949"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMQ9"/>
    <dbReference type="GeneID" id="8293786"/>
    <dbReference type="KEGG" id="lth:KLTH0G10912g"/>
    <dbReference type="eggNOG" id="KOG0118">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025000_4_0_1"/>
    <dbReference type="InParanoid" id="C5DMQ9"/>
    <dbReference type="OMA" id="RINWASK"/>
    <dbReference type="OrthoDB" id="8093034at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050502">
        <property type="entry name" value="Euk_RNA-bind_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025">
        <property type="entry name" value="OS02G0815200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025:SF1">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="67"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="154"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="321"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="231"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="389"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="19"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="238"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="413"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR25070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="436" mass="48219" checksum="A5B58E61146D95AC" modified="2009-07-28" version="1">MSETVAETRPEQPPVTADESASSTPAPAPAPEVAQTPVQTPESSTKPEEPTVTPANASRGGRETSDRILYVGNLDLAVTEEMLKQYFQVGGSIANVKILMDKNNKQANYAFVEFHQPHDANVAFQTLDGKQIENHVIKINWAFQSQQVSSEDTFNLFVGDLNVDVDDETLARTFKDIPTFIQAHVMWDMQTGRSRGYGFVSFGEQTQAQKAMEDNQGAVVNGRAIRINWASKREHNSHNNNPMNNRGGARRGGFRNHGNNQLRHQMPPMGMPGRGAMLPNMGMPSQPPMPQGAQPQGPIMPPQVPPQAVEAMMRSAPPRVTTVYIGNIPHFATEQDLIPLLQNFGFIVDFKHYPDRGCCFIKYGTHEQAAVCILTLGNFPFQGRNLRTGWGKEKPSYIPQPKPGQGASKMEQLPTQPIEQDPQSNTPAEQPQEEEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMY0</accession>
    <name>C5DMY0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0G12540p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0G12540g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555578.1">
        <property type="nucleotide sequence ID" value="XM_002555532.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMY0"/>
    <dbReference type="FunCoup" id="C5DMY0">
        <property type="interactions" value="232"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMY0"/>
    <dbReference type="GeneID" id="8293860"/>
    <dbReference type="KEGG" id="lth:KLTH0G12540g"/>
    <dbReference type="eggNOG" id="KOG0001">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010412_6_4_1"/>
    <dbReference type="InParanoid" id="C5DMY0"/>
    <dbReference type="OMA" id="YAGKQMA"/>
    <dbReference type="OrthoDB" id="428577at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="CDD" id="cd01806">
        <property type="entry name" value="Ubl_NEDD8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000306">
        <property type="entry name" value="Putative ubiquitin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038738">
        <property type="entry name" value="Nedd8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019954">
        <property type="entry name" value="Ubiquitin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019956">
        <property type="entry name" value="Ubiquitin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050158">
        <property type="entry name" value="Ubiquitin_ubiquitin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10666">
        <property type="entry name" value="UBIQUITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00348">
        <property type="entry name" value="UBIQUITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00299">
        <property type="entry name" value="UBIQUITIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="2">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR25141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="77" mass="8644" checksum="7723EB9E2DE6905A" modified="2009-07-28" version="1">MKLTVKTLTGKEIPVVVDPDDKVYRIKEYLEEKEGIPPTQQRLIFQGKQIDDDKSVSSANLSSGMQLHLVLTLRGGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMY1</accession>
    <name>C5DMY1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Pre-mRNA-splicing factor CLF1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0G12562g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in pre-mRNA splicing and cell cycle progression. Required for the spliceosome assembly and initiation of the DNA replication.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the crooked-neck family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555579.1">
        <property type="nucleotide sequence ID" value="XM_002555533.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMY1"/>
    <dbReference type="FunCoup" id="C5DMY1">
        <property type="interactions" value="1167"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMY1"/>
    <dbReference type="GeneID" id="8293861"/>
    <dbReference type="KEGG" id="lth:KLTH0G12562g"/>
    <dbReference type="eggNOG" id="KOG1915">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011554_1_0_1"/>
    <dbReference type="InParanoid" id="C5DMY1"/>
    <dbReference type="OMA" id="HIKVWIS"/>
    <dbReference type="OrthoDB" id="541719at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071007">
        <property type="term" value="C:U2-type catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000245">
        <property type="term" value="P:spliceosomal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055433">
        <property type="entry name" value="HAT_Syf1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055430">
        <property type="entry name" value="HAT_Syf1_CNRKL1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045075">
        <property type="entry name" value="Syf1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11246:SF3">
        <property type="entry name" value="CROOKED NECK-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11246">
        <property type="entry name" value="PRE-MRNA SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23231">
        <property type="entry name" value="HAT_Syf1_CNRKL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23233">
        <property type="entry name" value="HAT_Syf1_CNRKL1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Pre-mRNA-splicing factor Syf1-like N-terminal HAT-repeats" evidence="12">
        <location>
            <begin position="94"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="Pre-mRNA-splicing factor Syf1/CRNKL1-like C-terminal HAT-repeats" evidence="11">
        <location>
            <begin position="398"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="596"/>
            <end position="625"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF23231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF23233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR25142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="725" mass="84993" checksum="53002A1D958BC321" modified="2009-07-28" version="1">MPLGANIHSLLEFLYYFCVLWIYPLHFISTKVYSEGVELVKSKLNHMEPEPVQISADSLLRGAFDQKAPIKPTTKVEILDLEELKEYQRRKRSEFENVLKVKRHDIKQWARYAKFELDQRDMRRARSVFERALQINNAYVPLWIKYIDSELKARNVNHARNLLNRATNLLPRVGKLWLKYVIVEESLNNTDIVRQLFAKWCSLGPGKNAFDAYVDFEIRHGNFENVRKVYGRYVLAHPEISTWLKWVAFEKKHGDSDTTRQVLSLGLDTFSLYEISKDSDIASLVGAYAEWEATQQEYERSSALFDLASQRWPHNGDLERRRVQFEKMYGTSTNVNDSITSKRKREYEIALSNDPKDYDTWWIYLDLLQKHYPNQAIPGFHKSVTGNAPDGKVKNLSWERYIYLWLRCLTYFELDVSDLKETRRMYKRLIKEVIPHKSFTFAKVWVMYAKFELRQGDIMTARKILGQALGICPKNKIFKYYIELEIQLKEFDRVRKIYEQYIAFNALESDNWLAYAELEDNLGDEERARGIFHIALSDKVGLDTNERFKILEQLITFETNAGEYAKARQAYDALVQLSGYSPTTYIERAMFESTIPADNLSKDDQSQNMAEGDEDEPLDVTEANKRNSRSIFNKALDFYKEQGDKESRCAMLRALNSYEAVHGTVQTREAARKRLPKRRTLTKRENNVEVEYTDYEFPEDEEGEVGFSNNDKLLALAQKWKQGNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMY7</accession>
    <name>C5DMY7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="17">KLTH0G12716p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">KLTH0G12716g</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Cooperates for the ATP-dependent vacuolar transport of bilirubin and glutathione conjugates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555585.1">
        <property type="nucleotide sequence ID" value="XM_002555539.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DMY7">
        <property type="interactions" value="327"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMY7"/>
    <dbReference type="GeneID" id="8293868"/>
    <dbReference type="KEGG" id="lth:KLTH0G12716g"/>
    <dbReference type="eggNOG" id="KOG0054">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_27_3_1"/>
    <dbReference type="InParanoid" id="C5DMY7"/>
    <dbReference type="OMA" id="CFETGMR"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042592">
        <property type="term" value="P:homeostatic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18595">
        <property type="entry name" value="ABC_6TM_MRP1_2_3_6_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18603">
        <property type="entry name" value="ABC_6TM_MRP1_2_3_6_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000565">
        <property type="entry name" value="ABC bile acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000020">
        <property type="entry name" value="ABC metal ion transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000450">
        <property type="entry name" value="ABC transporter C family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000001">
        <property type="entry name" value="ATP-binding cassette subfamily C member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056227">
        <property type="entry name" value="TMD0_ABC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF443">
        <property type="entry name" value="MULTIDRUG-RESISTANCE LIKE PROTEIN 1, ISOFORM I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24357">
        <property type="entry name" value="TMD0_ABC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-1278">Translocase</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="5" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="74"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="171"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="278"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="336"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="415"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="439"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="517"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="555"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="937"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="977"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1066"/>
            <end position="1092"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1162"/>
            <end position="1183"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="16">
        <location>
            <begin position="291"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="15">
        <location>
            <begin position="620"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="16">
        <location>
            <begin position="938"/>
            <end position="1221"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="15">
        <location>
            <begin position="1258"/>
            <end position="1493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00053425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAR25148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1500" mass="168272" checksum="ECB0D8784E2195DE" modified="2009-07-28" version="1">MENVSLSSSSWRICDFCQDKEGLGPISKYGDFTPCFLDGVLLNATSLLMLAFGLKEIARLCRGPHPGIKFRRHWLLASRMTLVMFQIVLACIAIGKADSELEQQWASVSQRILGLASLCVALPLHWIEYHRSRVSNGVLLFFWLFEGLFTLFRAVHFKILLSYEEFLPVSHAGYVLTIFQSVTSFFILLLEAKFQKPQLGLADLSSSSRRKRNPYDSANIFSKISFSWMTQLMKTGYEKYLTETDLYNLPAEFGSADISRKFGQHWDYEVKHRAKPSLAWAMCVTFGGKMIVGACFKALYDILAFTQPQLLKSLIKFVTDYTDQNAFSQVPIVKGFMLSIGMFLVSVFQTSLLHQYFLRAFDTGMNAKSGLTSVIYQKALVLSNEASSASSTGDIVNLMSVDVQRIQDLTQWGQIIWSGPFQLILCLVSLYKLLGNSMWIGVIIMVITIPANSLIMRYQKKLQKVQMKYKDGRTRLISEILNNIKSLKLYAWEEPYRKKLDYVRNEKELKNLRRMGITNACASFQFNVIPFLVSCSTFGVFIWTQDAPLTTDLVFPALTLFNLLAFPLAVIPTAITAFVEASVSITRLQSFLTNEELQRDSVQRKSKVTKKGEVAVNVGADATFLWQRKPEYKVALKNINFAAKKGKLTCVVGKVGSGKSALIQAILGDLFRVKGFASVHGSIAYVSQVPWIMNGTVKDNILFGHKYDESFYTMTLKACALIVDLAVLPKGDQTLVGEKGISLSGGQKARLSLARAVYARADTYLLDDPLAAVDEHVTKHLVEHVLGPNGLLASKTKVLATNKITVLSIADHIALLENGEIIQQGSYDDVTSAKSSALSKIISTFGKKPEKSKTGENTVATTPELGAIAGSEIDLKKLDDKLIQEDTRSLRRASDATLRSLGFDDEEQPSLREHREQGKVKWDVYMEYAKACNPKHVLLFLAFVVLSMFLSVMSSVWLKHWSEVNTKYGFNPNSSKYLTVLFLLGVGSAISTLIQTVILWVYCTIHGSRYLHNIMADAVFKAPMSFFETTPIGRILNRFSNDIFKVDELLGRTFAQFFVNAIKVSFTIIVICFSTWQFILVILPMGTLYIYYQQYYLRTSRELRRLDSVTRSPIYAHFQETLGGISTIRGYNQQKRFVHINQARVDNNMSAYYPSVNANRWLAFRLEFIGSVIILAASSLSVLRLKQGALTPGMIGLSVSYALQITQSLNWIVRMTVEVETNIVSVERIKEYANLESEAPAVIESNRPSEDWPSQGSIKFENYSARYRPELELSLKDINLDIKPQERIGIVGRTGAGKSSLTLALFRIIEAASGRIYIDGLPIDSIGLRDLRHHLSIIPQDSQLFEGTIRENIDPTNEYTDEQIWRALELSHLKDHVKGLGKEELDAPLTEGGSNLSVGQRQLMCLARALLIPSKILVLDEATAAVDVETDQVIQETIRTAFKNRTILTIAHRINTILDSDRILVLDSGKVAEFDTPDNLLKNPESLFYSLCYEAGVLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DN28</accession>
    <name>C5DN28_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glutamate pyruvate transaminase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Glutamic--alanine transaminase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Glutamic--pyruvic transaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0G13662g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555626.1">
        <property type="nucleotide sequence ID" value="XM_002555580.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DN28"/>
    <dbReference type="FunCoup" id="C5DN28">
        <property type="interactions" value="458"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DN28"/>
    <dbReference type="GeneID" id="8293909"/>
    <dbReference type="KEGG" id="lth:KLTH0G13662g"/>
    <dbReference type="eggNOG" id="KOG0258">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014254_3_0_1"/>
    <dbReference type="InParanoid" id="C5DN28"/>
    <dbReference type="OMA" id="FGFECPP"/>
    <dbReference type="OrthoDB" id="1732682at2759"/>
    <dbReference type="UniPathway" id="UPA00528">
        <property type="reaction ID" value="UER00586"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042853">
        <property type="term" value="P:L-alanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1970:FF:000001">
        <property type="entry name" value="Alanine aminotransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000012">
        <property type="entry name" value="alanine aminotransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000010">
        <property type="entry name" value="Alanine aminotransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045088">
        <property type="entry name" value="ALAT1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11751">
        <property type="entry name" value="ALANINE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11751:SF29">
        <property type="entry name" value="ALANINE TRANSAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="10">
        <location>
            <begin position="129"/>
            <end position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00077894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00078532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00080525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR25189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="519" mass="57118" checksum="2FEE7F756D76B7AF" modified="2009-07-28" version="1">MMLSRQLTRTMPHSRLILRRLSAKAAVKSDLSQKALGSKKTLRDVNQCVLNAKYAVRGPIPLRAEELRHKLVEEPGSLPFNKIINANIGNPQQLDQKPLTFYRQVLSLLQNPALIESLPTGELHKLFKPDAVERAKKMLKQVGGSVGAYSASQGVAGFRQSVAEFIEERDGEPASANDIFLTAGASSAVSAILSMFCTGSENGALIPIPQYPLYTATLALNDAHALPYYLDEASGWSMDTEQVEQVVKDAIRKGVRPTCLVVINPGNPTGAILSPEAIEKILTVAAKYSLVVIADEVYQENGFDDADFVSMKKVLRVLQKTEPGKYDNVQLASLHSTSKGVSGECGQRGGYMELVGFSEEVKQVFLKLASISLCPVVTGQALVDLMVRPPKKGDASYELDRQERNAIHNSLHQKSTLLYETFQSLEGVSCQKPQGAMYLFPKLDLSRKAIRKAESLGMAPDEYYCKELLEATGICTVPGSGFRQVPGTYHLRTTFLAPGTEWVDSWKKFHQKFVDEHRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNB3</accession>
    <name>C5DNB3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-directed RNA polymerases I, II, and III subunit RPABC1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0G15598g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the archaeal Rpo5/eukaryotic RPB5 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555711.1">
        <property type="nucleotide sequence ID" value="XM_002555665.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNB3"/>
    <dbReference type="FunCoup" id="C5DNB3">
        <property type="interactions" value="1156"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNB3"/>
    <dbReference type="GeneID" id="8293997"/>
    <dbReference type="KEGG" id="lth:KLTH0G15598g"/>
    <dbReference type="eggNOG" id="KOG3218">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058320_0_0_1"/>
    <dbReference type="InParanoid" id="C5DNB3"/>
    <dbReference type="OMA" id="VRDRGYF"/>
    <dbReference type="OrthoDB" id="248779at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1340.10:FF:000002">
        <property type="entry name" value="DNA-directed RNA polymerases I, II, and III subunit RPABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.940.20:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerases I, II, and III subunit RPABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1340.10">
        <property type="entry name" value="RNA polymerase, Rpb5, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.940.20">
        <property type="entry name" value="RPB5-like RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00025">
        <property type="entry name" value="RNApol_Rpo5_RPB5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014381">
        <property type="entry name" value="Arch_Rpo5/euc_Rpb5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005571">
        <property type="entry name" value="RNA_pol_Rpb5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036710">
        <property type="entry name" value="RNA_pol_Rpb5_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000783">
        <property type="entry name" value="RNA_pol_subH/Rpb5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020608">
        <property type="entry name" value="RNA_pol_subH/Rpb5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035913">
        <property type="entry name" value="RPB5-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007129">
        <property type="entry name" value="PRK09570.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10535">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10535:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01191">
        <property type="entry name" value="RNA_pol_Rpb5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03871">
        <property type="entry name" value="RNA_pol_Rpb5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000747">
        <property type="entry name" value="RPB5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53036">
        <property type="entry name" value="Eukaryotic RPB5 N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55287">
        <property type="entry name" value="RPB5-like RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01110">
        <property type="entry name" value="RNA_POL_H_23KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="RNA polymerase Rpb5 N-terminal" evidence="8">
        <location>
            <begin position="6"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase subunit H/Rpb5 C-terminal" evidence="7">
        <location>
            <begin position="142"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR25274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="215" mass="25010" checksum="28D298A21ED0B058" modified="2009-07-28" version="1">MDQENERNVSRLWRAFKTVKEMVRDRGYFITQEEVDLSLEDFKAKYCDSMGRPQRKMMSFQSNPVEEALEKFPNMGSLWVEFCDEASVGIKTMKNFVIHISEKNFQTGIFVYQNGVTPSAMKLLPSVAPATIETFHEASLVVNITHHELVPKHIKLSDEEKKELLRRYRLKESQLPRIQRMDPVALYLGLKRGDVVKIIRKSETSGRYASYRVCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-04-02" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNS4</accession>
    <name>C5DNS4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">KLTH0G19426p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0G19426g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555872.1">
        <property type="nucleotide sequence ID" value="XM_002555826.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNS4"/>
    <dbReference type="STRING" id="559295.C5DNS4"/>
    <dbReference type="GeneID" id="8294167"/>
    <dbReference type="KEGG" id="lth:KLTH0G19426g"/>
    <dbReference type="HOGENOM" id="CLU_368036_0_0_1"/>
    <dbReference type="InParanoid" id="C5DNS4"/>
    <dbReference type="OMA" id="MPLANCH"/>
    <dbReference type="OrthoDB" id="4837779at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009791">
        <property type="term" value="P:post-embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003656">
        <property type="entry name" value="Znf_BED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052035">
        <property type="entry name" value="ZnF_BED_domain_contain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481:SF10">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02892">
        <property type="entry name" value="zf-BED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00614">
        <property type="entry name" value="ZnF_BED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50808">
        <property type="entry name" value="ZF_BED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BED-type" evidence="11">
        <location>
            <begin position="67"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="27"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR25435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="680" mass="76458" checksum="F8D810C1EE3D0BB1" modified="2009-07-28" version="1">MESVGKRARSGSHSEDGGRLSQLGSPDFHKPKVLKSSEKTSSKWLERSRLRIHFTVAFHGGQKTASCNYCGKVYQDGDSTSNLARHVTHRHTAEYKKTVEEHALSKTAHPATRPLAALTKPLSAAHAPPAAMSQAGNLSSLVVSSGRYFLPSGCHSCALEPPNFESDIYQHMENYAHSLDARFGLCEDLVVRSLKERLLKSTHINIQLELMQNTWGSHVAVFASFAPNLVDSEDSESFEQDEMPLANCHMLDIFELGSDKGESLSRKLYDAFKEFGIANKVGTITADIATSMAIISRGTTESLPTITCAQGLISVLFKCIFNELVRAPAFSTAFTKISDFAQKTIATPSLRASMETFGVPFLPTSIQSDSVHEWRVVKSFLDNQGKYLEWLSKSPINTALTPAACTLSQEQLKVLKHFVNCLSIFEVWATKLQNASSNTIMNAVLVYYSLNRFYASARSTLQRPDNEYPFFPVEYGTSREQRNQVLQGILTSQKIFEAQFDSFKRNPLFFAAAALNPVSKFESFKELMDEAERSARITELDSFFHGWLTGSQDSRLLEPAGEITVSSLTTTHVPDPEAEQMAVVDEWDFYKREPKLYGGSELDGVKWWYDHRSAYPQLFKLAVALYYTKISVRGSERLFSAKESLRRRERQNIQNENIKRSVILRDRLKSFRFVRSNDQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5E1X4</accession>
    <name>C5E1X4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cytosolic Fe-S cluster assembly factor NAR1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Cytosolic Fe-S cluster assembly factor nar1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Nuclear architecture-related protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0H00396g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NARF family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555897.1">
        <property type="nucleotide sequence ID" value="XM_002555851.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E1X4"/>
    <dbReference type="FunCoup" id="C5E1X4">
        <property type="interactions" value="404"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E1X4"/>
    <dbReference type="GeneID" id="8294208"/>
    <dbReference type="KEGG" id="lth:KLTH0H00396g"/>
    <dbReference type="eggNOG" id="KOG2439">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018240_0_1_1"/>
    <dbReference type="InParanoid" id="C5E1X4"/>
    <dbReference type="OMA" id="VMPCTCK"/>
    <dbReference type="OrthoDB" id="10253113at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1780">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.950.10">
        <property type="entry name" value="Fe-only Hydrogenase (Larger Subunit), Chain L, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050340">
        <property type="entry name" value="Cytosolic_Fe-S_CAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009016">
        <property type="entry name" value="Fe_hydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004108">
        <property type="entry name" value="Fe_hydrogenase_lsu_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11615">
        <property type="entry name" value="NITRATE, FORMATE, IRON DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02906">
        <property type="entry name" value="Fe_hyd_lg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53920">
        <property type="entry name" value="Fe-only hydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Iron hydrogenase large subunit C-terminal" evidence="7">
        <location>
            <begin position="92"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR30035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="463" mass="50755" checksum="505734C00591E7E1" modified="2009-07-28" version="1">MSSLLSEKDLNDFISPGLACVKPAEPIEKGEKQEGDLEVGKESNDMGKVSISLQDCLACSGCITSSEEILLSQQSHTVFLEAWKNLARDTALVVSIPPQCRLSLAHYYDMSLESFDKSFLTLMKEKFGARYIVGTQIGRNITVNQTNKLLTERKSLSSSKKPALCAVCPGFVLYAEKTKPELVPYLLDVKSPQQITGALLKKAIPNIYHLTIMPCFDKKLEASRKDGEGEVDCVITPREFATMLQEQDLKLTDFLTSTDETVLKQSCSPPGWDISVHWASNEGSASGGYAYQYILHQKSLHPGSCISTITGKNSDLKEFRLMDVSNENVIASSSELYGFRNIQNMVRKLTSSGSASRNIKVLRKRATTSSNNGSSFGSAVDPSKTDFIEVMACPGGCINGGGLLNGEANSSRRRALAKSLELEYGKVIPKVSCSDHPRDDSTYKYKFSAIEKSQDVLTVGNTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>C5E256</accession>
    <name>C5E256_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0H02288p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0H02288g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555979.1">
        <property type="nucleotide sequence ID" value="XM_002555933.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E256"/>
    <dbReference type="STRING" id="559295.C5E256"/>
    <dbReference type="GeneID" id="8294292"/>
    <dbReference type="KEGG" id="lth:KLTH0H02288g"/>
    <dbReference type="eggNOG" id="KOG2953">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_850116_0_0_1"/>
    <dbReference type="InParanoid" id="C5E256"/>
    <dbReference type="OMA" id="ASKSMIA"/>
    <dbReference type="OrthoDB" id="278430at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006012">
        <property type="term" value="P:galactose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02642">
        <property type="entry name" value="R3H_encore_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.50">
        <property type="entry name" value="R3H-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001374">
        <property type="entry name" value="R3H_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036867">
        <property type="entry name" value="R3H_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051937">
        <property type="entry name" value="R3H_domain_containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15672">
        <property type="entry name" value="CAMP-REGULATED PHOSPHOPROTEIN 21 RELATED R3H DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15672:SF8">
        <property type="entry name" value="PROTEIN ENCORE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01424">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00393">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82708">
        <property type="entry name" value="R3H domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51061">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="R3H" evidence="3">
        <location>
            <begin position="27"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="139"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="260"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="178"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="271"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="294"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR30117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="327" mass="36718" checksum="E374F7413B8AB081" modified="2009-07-28" version="1">MTSNNGMAIGKLTPALVAALFERAHDKQFIIQLENSMVEFMGSPAEAYQLEPMNSYYRLLAHHVAEYHGLGHSLSRNNDACVVVFKGETFLRDKSIVLLQNLDPSCVTNAVDTQRSESKQSASKSMIAQETRQVYNSAGLKPLAAVSEKEGTRTPETPSTPKKPIPIEDDSPQPHQFETSRYRFRQQSEKPRRRKNYKGSTNRTAQPVYFPPPPAFPVPYMLYNPYPMMFVPPERQFSQFAPVAPVGQYPNPNPFIYGHPAHNKVEPYGPNGSSSTFSSMRTYKTEDSESLESLSKDASEKQEPRMSRKDSETSVGKISDKMDKLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>C5E299</accession>
    <name>C5E299_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">mRNA-capping enzyme subunit beta</fullName>
            <ecNumber evidence="8">3.6.1.74</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">mRNA 5'-phosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">mRNA 5'-triphosphate monophosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0H03234g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">First step of mRNA capping. Converts the 5'-triphosphate end of a nascent mRNA chain into a diphosphate end.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 5'-end triphospho-ribonucleoside in mRNA + H2O = a 5'-end diphospho-ribonucleoside in mRNA + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:67004"/>
            <dbReference type="Rhea" id="RHEA-COMP:17164"/>
            <dbReference type="Rhea" id="RHEA-COMP:17165"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:167616"/>
            <dbReference type="ChEBI" id="CHEBI:167618"/>
            <dbReference type="EC" id="3.6.1.74"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:67005"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="8">Heterodimer. The mRNA-capping enzyme is composed of two separate chains alpha and beta, respectively a mRNA guanylyltransferase and an mRNA 5'-triphosphate monophosphatase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the fungal TPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.74" evidence="8"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556022.1">
        <property type="nucleotide sequence ID" value="XM_002555976.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E299"/>
    <dbReference type="FunCoup" id="C5E299">
        <property type="interactions" value="87"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E299"/>
    <dbReference type="GeneID" id="8294335"/>
    <dbReference type="KEGG" id="lth:KLTH0H03234g"/>
    <dbReference type="eggNOG" id="ENOG502RZAX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037653_0_0_1"/>
    <dbReference type="InParanoid" id="C5E299"/>
    <dbReference type="OMA" id="DWVYATI"/>
    <dbReference type="OrthoDB" id="272147at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031533">
        <property type="term" value="C:mRNA capping enzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140818">
        <property type="term" value="F:mRNA 5'-triphosphate monophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004651">
        <property type="term" value="F:polynucleotide 5'-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006370">
        <property type="term" value="P:7-methylguanosine mRNA capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07470">
        <property type="entry name" value="CYTH-like_mRNA_RTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.100.10">
        <property type="entry name" value="mRNA triphosphatase Cet1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040343">
        <property type="entry name" value="Cet1/Ctl1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033469">
        <property type="entry name" value="CYTH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004206">
        <property type="entry name" value="mRNA_triPase_Cet1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037009">
        <property type="entry name" value="mRNA_triPase_Cet1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28118:SF1">
        <property type="entry name" value="POLYNUCLEOTIDE 5'-TRIPHOSPHATASE CTL1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28118">
        <property type="entry name" value="POLYNUCLEOTIDE 5'-TRIPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02940">
        <property type="entry name" value="mRNA_triPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55154">
        <property type="entry name" value="CYTH-like phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0506">mRNA capping</keyword>
    <keyword evidence="4 8" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="mRNA triphosphatase Cet1-like" evidence="10">
        <location>
            <begin position="210"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="17"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="51"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="62"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="75"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="96"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR30160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="479" mass="53809" checksum="A83714044427F095" modified="2009-07-28" version="1">MSSSKPNFSRGLSLEDLVNHDERSDAKAGQKSNPLPPAEVKHRMSVDGLGSEQNGSASSSRVPREQRGPRRNADGDEETDTDEEVGGPGDIVFETGDFKFDYEKQEGAGKNRDARAGREDTSSSSPSSKAKGGQDVKDIFQERASSQSKRNAIKKDLNVLSEIAATAKPTRYNTAPIWAQKWKPTVKALQSIDTKDLNIDASFTNIIPDDDLTKSVQDWVYATLVSIPPDQRQYVEMEMKFGIIVEGSDSNRVSPPVSSQTVYTDMDAHLTPDVDERVFTEINRYVKGISELSEYTGKFNIIESHTKDLLYRVGMSTQRPRFLRMSRDVKTGRVGEFIEKRHVSQLLLFSPKDSYDVKISINLELPVPDNDPPEKYKDNTPVNTRTKQRISYIHNDSCTRMDITKVANHNQGVKQRHTESTHEIELEVNTAALLTAFEHIGNNSKEYASILRTFLNNGTIIRRKLTSLSYEIFEGQKKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2C4</accession>
    <name>C5E2C4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">U2 small nuclear ribonucleoprotein A'</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0H03806g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the U2 small nuclear ribonucleoprotein A family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556047.1">
        <property type="nucleotide sequence ID" value="XM_002556001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2C4"/>
    <dbReference type="FunCoup" id="C5E2C4">
        <property type="interactions" value="1371"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2C4"/>
    <dbReference type="GeneID" id="8294360"/>
    <dbReference type="KEGG" id="lth:KLTH0H03806g"/>
    <dbReference type="eggNOG" id="KOG1644">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061027_3_0_1"/>
    <dbReference type="InParanoid" id="C5E2C4"/>
    <dbReference type="OMA" id="CHLEDYR"/>
    <dbReference type="OrthoDB" id="433501at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030620">
        <property type="term" value="F:U2 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000629">
        <property type="entry name" value="U2 snRNP component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044640">
        <property type="entry name" value="RU2A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10552">
        <property type="entry name" value="U2 SMALL NUCLEAR RIBONUCLEOPROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10552:SF6">
        <property type="entry name" value="U2 SMALL NUCLEAR RIBONUCLEOPROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR30185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="232" mass="25951" checksum="1AAB09226E585A22" modified="2009-07-28" version="1">MKITPSVVLDAPTYYVSHFNGVYDTEKCVVIRDLGIECDDEAMPAVLAKLPKPTHVLDLTNNELNQIPDLSAHDEITTLLLGRNRISKIDGRRLPRRLRRLVLSNNGVEQLAALEGLRDAPKTLENLSLRGNSVCHLEDYRVFALSRAPQLKTLDFTKISDQERNAGCKFRSSPGKDIAPSRQKFKDKNEEIMDVIVGKMSDEVRNRLKDELAAASTLDEIERIESLLSGGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2C9</accession>
    <name>C5E2C9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">60S ribosome subunit biogenesis protein NIP7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0H03916g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Required for proper 27S pre-rRNA processing and 60S ribosome subunit assembly.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Interacts with pre-ribosome complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the NIP7 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556052.1">
        <property type="nucleotide sequence ID" value="XM_002556006.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2C9"/>
    <dbReference type="FunCoup" id="C5E2C9">
        <property type="interactions" value="1172"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2C9"/>
    <dbReference type="GeneID" id="8294365"/>
    <dbReference type="KEGG" id="lth:KLTH0H03916g"/>
    <dbReference type="eggNOG" id="KOG3492">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097217_0_0_1"/>
    <dbReference type="InParanoid" id="C5E2C9"/>
    <dbReference type="OMA" id="LISMGTC"/>
    <dbReference type="OrthoDB" id="27490at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902626">
        <property type="term" value="P:assembly of large subunit precursor of preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd21146">
        <property type="entry name" value="Nip7_N_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21151">
        <property type="entry name" value="PUA_Nip7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.130.10:FF:000002">
        <property type="entry name" value="60S ribosome subunit biogenesis protein NIP7 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.220:FF:000001">
        <property type="entry name" value="60S ribosome subunit biogenesis protein NIP7 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="entry name" value="PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040598">
        <property type="entry name" value="NIP7_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055359">
        <property type="entry name" value="Nip7_N_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016686">
        <property type="entry name" value="Ribosomal_synth_fac_NIP7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005155">
        <property type="entry name" value="UPF0113_PUA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23415">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASES REGULATORY SUBUNIT/60S RIBOSOME SUBUNIT BIOGENESIS PROTEIN NIP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17833">
        <property type="entry name" value="pre-PUA_NIP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03657">
        <property type="entry name" value="UPF0113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017190">
        <property type="entry name" value="Rbsml_synth_fac_NIP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88802">
        <property type="entry name" value="Pre-PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="PUA" evidence="8">
        <location>
            <begin position="95"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00054591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR017190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR30190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="181" mass="20469" checksum="86AAD15CBD562BF7" modified="2009-07-28" version="1">MRQLTEDETKVVFEKLAGYIGRNIKFLVDNKDQPFVFRLQKDRVYYVPEHIAKLATSVARPNLMSVGLCLGKFTKTGKFRLHITALTILAQHAKYKIWIKPNGEMPFLYGNHVLKAHVGKMSEDIPEHAGVIVFSMHDVPLGFGVSSKSTSESRDVQPTGIVAFRQADIGEYLRDEDTLFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2F7</accession>
    <name>C5E2F7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0H04576p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0H04576g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556080.1">
        <property type="nucleotide sequence ID" value="XM_002556034.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2F7"/>
    <dbReference type="FunCoup" id="C5E2F7">
        <property type="interactions" value="197"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2F7"/>
    <dbReference type="GeneID" id="8294393"/>
    <dbReference type="KEGG" id="lth:KLTH0H04576g"/>
    <dbReference type="eggNOG" id="ENOG502QT39">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_490204_0_0_1"/>
    <dbReference type="InParanoid" id="C5E2F7"/>
    <dbReference type="OMA" id="EHYCLFT"/>
    <dbReference type="OrthoDB" id="3992718at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030896">
        <property type="term" value="C:checkpoint clamp complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071479">
        <property type="term" value="P:cellular response to ionizing radiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000076">
        <property type="term" value="P:DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031573">
        <property type="term" value="P:mitotic intra-S DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000725">
        <property type="term" value="P:recombinational repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.70.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026217">
        <property type="entry name" value="Ddc1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046938">
        <property type="entry name" value="DNA_clamp_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007268">
        <property type="entry name" value="Rad9/Ddc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15237:SF0">
        <property type="entry name" value="CELL CYCLE CHECKPOINT CONTROL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15237">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02063">
        <property type="entry name" value="DNADAMAGECP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55979">
        <property type="entry name" value="DNA clamp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="396"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="510"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="406"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="425"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="510"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR30218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="544" mass="62437" checksum="1F1D5420D964D77C" modified="2009-07-28" version="1">MIFKASIIKAENHSVWRRAVQMLSTLHEDIKLTITSSELIVWSMNSADTSMSQIRFKRNFFDEYEFQPENTVFGEDGLQQVEDRALVVHKLYSFKTNGKHLSVLFRKPENDNIKSLELAIHNGAACPESLANKLQITIHTENLMLKEYVPHIIPIKYDPIVINLRYKKRFLEVYGSSSETQEEQLDPRLLEIFRKFDRELSESYFNNDIVSNTKRSRDLVPEDEINYLCCNIQLLKNFIDSCNTGATEEVKLEVSLSKLCLTAFTRGVYSKNNDILRNAMRASNTVGTSDLEHYCLFTTTGDDDQRRPPSKVVSFGMKDFRNFINTVAFWRNDQEIHMWFCRPGDPVFLELDKDDLCLELVQVTSSNEDLVPSGNKIKTQTTSPLKEIVHIKLGSPRKSPLKGLTPSKTPSSNETTSPLKPKTLFVKEKSFDADQQRWKNPVRGKRLPDSQEEIPKSLHHAPNEPAFKAQRTQTTIEWGQSVPESANDAAATALDQRDLLKQEKRKFLQNIRNEREKDVEKDEPTQGVDEFGPTQVDKPKGLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2J0</accession>
    <name>C5E2J0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0H05390p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0H05390g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556113.1">
        <property type="nucleotide sequence ID" value="XM_002556067.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2J0"/>
    <dbReference type="SMR" id="C5E2J0"/>
    <dbReference type="FunCoup" id="C5E2J0">
        <property type="interactions" value="1032"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2J0"/>
    <dbReference type="GeneID" id="8294428"/>
    <dbReference type="KEGG" id="lth:KLTH0H05390g"/>
    <dbReference type="eggNOG" id="KOG1426">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005210_4_0_1"/>
    <dbReference type="InParanoid" id="C5E2J0"/>
    <dbReference type="OMA" id="IFVWGTG"/>
    <dbReference type="OrthoDB" id="61110at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051553">
        <property type="entry name" value="Ran_GTPase-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058923">
        <property type="entry name" value="RCC1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45982">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45982:SF1">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00625">
        <property type="entry name" value="RCC1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="47"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="RCC1-like" evidence="5">
        <location>
            <begin position="49"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="104"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="183"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="239"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="292"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="348"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="412"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="130"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="132"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR30251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="481" mass="53072" checksum="C14367EC52F2A150" modified="2009-07-28" version="1">MSKRSASPPLTPGKRARKHLVSTIHKHYELINSQDDYKYMYLSLQPLDIFCWGTGSMCELGLGPSAKNKEVKRPRLNPYLPRDQAKIVSFAVGGMHVLALDQDNHVWSWGTNDSGALGRDTSHANEQLKDMDADDSSDDEDGDLNDQESTPIKLPDESLPLKDHKVVQLAATDNLSCVLLDNGEVYAWGTFRCNEGILGFYKDTIKIQNTPWKVPSFTTSKVVQMAAGKDHILFLDEGGVVYAWGNGQQYQLGRKIMERSRLRTLDPRAFGLDKVKYIATGENHSFALTRDGRLYSWGLNQFGQCGVSEEVDDGALVTVPTEVLLPEGTRVKMVSAGEHHSMVLTEEGELYTFGRLDMFEIGIPKAKLPEQTYIDAHGKPRAVPVPTKLVEVPRFKNIAAGSHHSLAISEDGVVFSWGFGETYAVGLGPSGDDIEVPTRIKNTATQDHDIIFVGGGGQFSVSGGIKLNDQDAEKRADKYDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2T7</accession>
    <name>C5E2T7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">LSM complex subunit LSM5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LSM5</name>
        <name evidence="13" type="ordered locus">KLTH0H07612g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Plays a role in U6 snRNP assembly and function. Binds to the 3' end of U6 snRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">LSm subunits form a heteromer with a doughnut shape.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556210.1">
        <property type="nucleotide sequence ID" value="XM_002556164.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2T7"/>
    <dbReference type="FunCoup" id="C5E2T7">
        <property type="interactions" value="708"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2T7"/>
    <dbReference type="GeneID" id="8294528"/>
    <dbReference type="KEGG" id="lth:KLTH0H07612g"/>
    <dbReference type="eggNOG" id="KOG1775">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076902_6_0_1"/>
    <dbReference type="InParanoid" id="C5E2T7"/>
    <dbReference type="OMA" id="NNICTLI"/>
    <dbReference type="OrthoDB" id="429711at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990726">
        <property type="term" value="C:Lsm1-7-Pat1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005688">
        <property type="term" value="C:U6 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01732">
        <property type="entry name" value="LSm5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000067">
        <property type="entry name" value="U6 snRNA-associated Sm-like protein LSm5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033871">
        <property type="entry name" value="LSm5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20971">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20971:SF0">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8 11" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6 11" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Sm" evidence="12">
        <location>
            <begin position="7"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU365055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR30348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="81" mass="9050" checksum="0092CFB1590BB71A" modified="2009-07-28" version="1">MSIAEVLPLEIIDKTVGQPVWVMLTSHREFAGTLVGYDDFVNVVLEDVIEYDHDQEIKRHAGKMLLSGNNISMLVPGGKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5E301</accession>
    <name>C5E301_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Regulatory protein MIG1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">KLTH0H09196g</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Involved in glucose repression of glucose metabolism genes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the creA/MIG C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556274.1">
        <property type="nucleotide sequence ID" value="XM_002556228.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E301"/>
    <dbReference type="FunCoup" id="C5E301">
        <property type="interactions" value="645"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E301"/>
    <dbReference type="GeneID" id="8294599"/>
    <dbReference type="KEGG" id="lth:KLTH0H09196g"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047856_0_0_1"/>
    <dbReference type="InParanoid" id="C5E301"/>
    <dbReference type="OMA" id="KRIHTNP"/>
    <dbReference type="OrthoDB" id="654211at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000433">
        <property type="term" value="P:carbon catabolite repression of transcription from RNA polymerase II promoter by glucose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000089">
        <property type="entry name" value="DNA-binding protein creA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000152">
        <property type="entry name" value="DNA-binding protein creA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051007">
        <property type="entry name" value="creA/MIG_C2H2-ZnF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47428">
        <property type="entry name" value="REGULATORY PROTEIN MIG1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47428:SF1">
        <property type="entry name" value="REGULATORY PROTEIN MIG1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="26"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="54"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="67"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="187"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="235"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="353"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="95"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="132"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="187"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="197"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="243"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="282"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="297"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="318"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="359"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00056233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00068528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAR30412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="460" mass="49398" checksum="1268CA0AB9C09C78" modified="2009-07-28" version="1">MSSTDDTKGNKKRRSPNSGPDSPRPYICPICSRAFHRLEHQTRHIRTHTGEKPHACDFPGCTKKFSRSDELTRHKRIHTNPHPKGKRGRKKKTETQSSGADTAVQQGNLPRERATRPAPTFQLGGEDDDASDVNNPASSPEDQSMGILANAALKFQARSTDTPLHSVRSLPSLKTLEANGMRVASPPGLAGAALPPHNSSTGNLQSVGSLLPARSTPSRLKLNVLSSLQRMTPLAPVQAGNPGTAQYSSQTASASNSGTTTPYQSPEHPEYLGSCPPRHKTYSSSASLTSISNLITTDDEDREFDEEEMGRARKKSRTTTPTISRSHSQTNLSSRTGTFTNLASLNGVGQPPGITGILSSRPSSSDFSSELNQRLQSVQRSRSSQFLDRYASTPSVALSGAPSAKFTPLGTPPQLASPPNARAVPTDLQGHSQSSHCIKTESEESMLPPIRSLDLQFPTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>C5E348</accession>
    <name>C5E348_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0H10362p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0H10362g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556321.1">
        <property type="nucleotide sequence ID" value="XM_002556275.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E348"/>
    <dbReference type="FunCoup" id="C5E348">
        <property type="interactions" value="85"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E348"/>
    <dbReference type="GeneID" id="8294652"/>
    <dbReference type="KEGG" id="lth:KLTH0H10362g"/>
    <dbReference type="eggNOG" id="KOG2911">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021165_2_0_1"/>
    <dbReference type="InParanoid" id="C5E348"/>
    <dbReference type="OMA" id="NEQMATT"/>
    <dbReference type="OrthoDB" id="10250120at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000815">
        <property type="term" value="C:ESCRT III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005771">
        <property type="term" value="C:multivesicular body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006900">
        <property type="term" value="P:vesicle budding from membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761:SF96">
        <property type="entry name" value="BCDNA.GH08385"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25880">
        <property type="entry name" value="WHD_CHMP7_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="413"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="369"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="432"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR30459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="441" mass="50405" checksum="759EA01B2D3F332A" modified="2009-07-28" version="1">MRDTTLSKSRLTSLYSDFRKLKDLNPEGYEANVRYWRHLLLDEVFSDKLICQCGSELLKELSDKELGPPKCLDTALDSLISDGLLIAKERFKRNNNSKILKAFSWTIGYILAGRSQATTRTSEGANYLRDADFVVLPTTKRKSLQAEQRMRSSICKKAIRYSDLIFSKLEFCKIVGMEENLQGWNEFEIVLMYMETYMGVIVTDCNTVKVCTRDMAPLLAKFDFNFISENDRNIASVKETYSILQFQVSMLEDRIRERKQVLAECIKSDKPKDLQRLHLRAKKLLERNLTTACRNLQNIETLKSDIEMSLDNVNLRNTLLASRDVMSNVSAELGDTESVEKLLDEISLERSKNEEISDILAGKLDDQNDEQLNEELEELDAEVKKEQEVTRELSKLKISSYQGFGPEEVALVAGRESPVEEESRADLGPSESKNKEPILST</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3L2</accession>
    <name>C5E3L2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Nuclear pore complex protein Nup85</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0H14476g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Functions as a component of the nuclear pore complex (NPC).</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the nuclear pore complex (NPC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
            <location evidence="1 9">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the nucleoporin Nup85 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556485.1">
        <property type="nucleotide sequence ID" value="XM_002556439.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3L2"/>
    <dbReference type="FunCoup" id="C5E3L2">
        <property type="interactions" value="165"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3L2"/>
    <dbReference type="GeneID" id="8294847"/>
    <dbReference type="KEGG" id="lth:KLTH0H14476g"/>
    <dbReference type="eggNOG" id="KOG2271">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019986_0_0_1"/>
    <dbReference type="InParanoid" id="C5E3L2"/>
    <dbReference type="OMA" id="VKHYSWM"/>
    <dbReference type="OrthoDB" id="17644at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031080">
        <property type="term" value="C:nuclear pore outer ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011502">
        <property type="entry name" value="Nucleoporin_Nup85"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13373">
        <property type="entry name" value="FROUNT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13373:SF21">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07575">
        <property type="entry name" value="Nucleopor_Nup85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 9" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="7 9" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0811">Translocation</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR30623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="739" mass="83359" checksum="6EC69A699EFC8883" modified="2009-07-28" version="1">MTANILGEKSDLFMDVDNLDFLKDSTTDINDESYMDEDNFGIDEVSGAIETQFEGEQPIKEPLSSFRAEGKALQFRVGPVLSRSLGFVSGKCQDASESKLYTPNFHGIDTTENYKQYIMTCFEIYKNLGEDRFFSVPTMGLINHSAPMEHSQAVNMAIDAMSDELEVFSGTQKPARAAELEECLAIINCSRALHFTDGNDNEEANSAVLKQLTQWVNRADGEPSEEAVAEAFSGSVSYKSPLFWRLVNQLLLRGLFDQAIGVLERSHLLKDIENTCEITFNVLNDAISLLKQYPFELGEAFREWKATALQISQFFVDSTTNVDPELRDFVSDMILLMSGNQSKTLQYSRTWYESFCGLMLFYIPTLELTEEYLQISLKAHPLDVCNNWEQACVYVIQGKLYAVLPILESLDYCTAVFYSAICEAKGLLENSSTLMAHSNGSEGLEGDLFSSNNSMACYLMHEFALELCCYEDKNLWSVGIGILALSPSDNDSAKRLAIAELLPHYPFQNNDDIEWLLTLCAKWRLPDVAKTIYRILGNKVLYENNVIEAMTNFSKAGEFEWVKHYSWMIFEASAMKGAPLEDMIIDAIVSGEAETEVAPEILESVMTNAMRQTLSPYAVLYQFYQQRVAHDWEGALSSLVSLLEFRYMPKHYISLLVAQFLYPTFLKDDTAAISEDMVIRIIKTLDRVSTDDKSVALYEALKSGEGRDDEGLPKQLGDLLPLVRQRLSYKICQEYMSVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>C5DB80</accession>
    <name>C5DB80_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0A00374p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0A00374g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551479.1">
        <property type="nucleotide sequence ID" value="XM_002551433.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DB80"/>
    <dbReference type="FunCoup" id="C5DB80">
        <property type="interactions" value="28"/>
    </dbReference>
    <dbReference type="GeneID" id="8290269"/>
    <dbReference type="KEGG" id="lth:KLTH0A00374g"/>
    <dbReference type="eggNOG" id="ENOG502RCZR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065853_0_1_1"/>
    <dbReference type="InParanoid" id="C5DB80"/>
    <dbReference type="OMA" id="AKANPQW"/>
    <dbReference type="OrthoDB" id="2101473at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007396">
        <property type="entry name" value="TR_PAI2-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35802">
        <property type="entry name" value="PROTEASE SYNTHASE AND SPORULATION PROTEIN PAI 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35802:SF1">
        <property type="entry name" value="PROTEASE SYNTHASE AND SPORULATION PROTEIN PAI 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04299">
        <property type="entry name" value="FMN_bind_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR21037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="240" mass="27738" checksum="1970C0020784383E" modified="2009-07-28" version="1">MYIPKLTSVDDFDKQARTIKDHPLGILFSYSAPKIALTDFLLGDKIDKPVDSEMCATHVPFFLQKSQDGSCRLVAHLAGKNQQIKLLKENPSCMVVFQGPNSYVTPAWYPEKEETHKFVPTWDYSCVHVYGKAKIIEEKEWLLKMLDSLTDQEENKRPDRDEFESKWKVEQTNQKYLDSLIKGIVGLEIEVSHIQSKLKVHQNQNPKNVNGVLRGYEMEMGDDTAKQMCKLVRENYPREL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBB8</accession>
    <name>C5DBB8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0A01254p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0A01254g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551517.1">
        <property type="nucleotide sequence ID" value="XM_002551471.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBB8"/>
    <dbReference type="FunCoup" id="C5DBB8">
        <property type="interactions" value="258"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBB8"/>
    <dbReference type="GeneID" id="8290309"/>
    <dbReference type="KEGG" id="lth:KLTH0A01254g"/>
    <dbReference type="eggNOG" id="ENOG502RNWJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030906_0_0_1"/>
    <dbReference type="InParanoid" id="C5DBB8"/>
    <dbReference type="OMA" id="QPIGWLC"/>
    <dbReference type="OrthoDB" id="428577at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060963">
        <property type="term" value="P:positive regulation of ribosomal protein gene transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052146">
        <property type="entry name" value="HOT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022210">
        <property type="entry name" value="TF_GCR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37784">
        <property type="entry name" value="PROTEIN MSN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37784:SF8">
        <property type="entry name" value="PROTEIN MSN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12550">
        <property type="entry name" value="GCR1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription activator GCR1-like" evidence="2">
        <location>
            <begin position="190"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="132"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR21075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="294" mass="33296" checksum="3E0B255CB666DEDF" modified="2009-07-28" version="1">MDDVSPLIARISGLESQIAMYEKLFQTLSAKLDHHFKKYDTVVNAQQQQINSLTDVLFTLLNDRCRYADIQREKLGNTLAGVASTGASLSGVVATAGLGRQPKQATGPQTSSRHVSGLQSDHQSASLYAEDTPSRREDERRSSDSYYSDNSVTSAESDESEDRENSELRPELHESADAAHKKRKLFDRKFRFLASPQSVAEVWKEYTEGLAGQPSLKEMESTYRSSWRRDAAVSRKFYRRKVLCRAIETGLARGYALEEIIDMLESHRQQREGRQDKLPIGWLCRRSNLPSIFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBK2</accession>
    <name>C5DBK2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0A03300p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0A03300g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sirtuin family. Class I subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551601.1">
        <property type="nucleotide sequence ID" value="XM_002551555.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBK2"/>
    <dbReference type="STRING" id="559295.C5DBK2"/>
    <dbReference type="GeneID" id="8290401"/>
    <dbReference type="KEGG" id="lth:KLTH0A03300g"/>
    <dbReference type="eggNOG" id="KOG2684">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023643_3_1_1"/>
    <dbReference type="InParanoid" id="C5DBK2"/>
    <dbReference type="OMA" id="YHYWREL"/>
    <dbReference type="OrthoDB" id="424302at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017136">
        <property type="term" value="F:histone deacetylase activity, NAD-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1600.10">
        <property type="entry name" value="SIR2/SIRT2 'Small Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050134">
        <property type="entry name" value="NAD-dep_sirtuin_deacylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003000">
        <property type="entry name" value="Sirtuin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026591">
        <property type="entry name" value="Sirtuin_cat_small_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026590">
        <property type="entry name" value="Ssirtuin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085:SF10">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02146">
        <property type="entry name" value="SIR2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50305">
        <property type="entry name" value="SIRTUIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="Deacetylase sirtuin-type" evidence="8">
        <location>
            <begin position="1"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="182"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="185"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="209"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="219"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="147"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="250"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR21159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="363" mass="40808" checksum="65E3B24A70FCE5E6" modified="2009-07-28" version="1">MAESDGQMQQFQRYLSKARKILCIVGSGVSASSGIATYQLQKGSWHGYTALDLATPEAFQNDPGLVWLFYSNRRYEALRAKPNEGHYALAELCRRYPSGISRRGDSASGQHPPASVNDKKKVLVVSQNVDGLHQRAGHPPESLVELHGSVFGLRCTQFFCNYRSRNSNDRFLTPALYRYTPRNRGANKKRTKRVSLHDGTETKKRRLDSTANGVQGQETDLEPPSSEEESERPEMFPTLEPGDLPSCPQCHQGILRPAVVWYGESLPLDQMDEVDQFFNKGGPVDLVLVIGTSGKVWPAMGYVERAKKSGSRVAIFNRVIEDLDQVRDSKDIWGFQGDAAQLLPKALKPLIGERYKPRDWRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBL7</accession>
    <name>C5DBL7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0A03630p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0A03630g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551616.1">
        <property type="nucleotide sequence ID" value="XM_002551570.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBL7"/>
    <dbReference type="FunCoup" id="C5DBL7">
        <property type="interactions" value="103"/>
    </dbReference>
    <dbReference type="GeneID" id="8290416"/>
    <dbReference type="KEGG" id="lth:KLTH0A03630g"/>
    <dbReference type="eggNOG" id="ENOG502S7IH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_454197_0_0_1"/>
    <dbReference type="InParanoid" id="C5DBL7"/>
    <dbReference type="OMA" id="HINTARK"/>
    <dbReference type="OrthoDB" id="4036011at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="290"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="581"/>
            <end position="600"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="601" mass="66177" checksum="CC9A1B482FEC3863" modified="2009-07-28" version="1">MHGPLELFPSLRQRLLSCMFKPQIFAPHAVFWFSTWMLYFLFQDETASLSSTASLAAAGATLVTTTATATATMTATTTKTIAYTSSVETEAAGANITSAVETFLSLKANDSARQVATYIEGFNENCKLKLRLWNASLETQLGFWEREYESLKAYNNTIFSNLLEQAQTINSSVDYLSNSGFLVASDSQSSVLRGLSLNTSFLQSVFGNVSSNLGKIRNLTLPAAPTATASALSASEILDSITSNKTTSNFTSMLGVLGSDKKIRTTLLVKRDSASDTSGKYKQKAIRISSALAATYVAATFAFVCLEYVTYKIEVRGAQDTLQHQLECIDELYKGETVISVARIRAHESFQQLLICYNDFAKHPVTYTFTEMALELLRRPLSKTWSPVAVAKFVKAYNWWLTTNGLHVFCLFLAAVVEGQILRAFLDVSHSTHPTSLYPRSKFSEAALSTKTLGSISSSCALFESTVNAEINSVVHERLWNASRGNVTQINSLLDEYFSNVNATLKDQVPAIDMNMSYWSSELNSEQTFNFTSFSETLAHEIYEVVAPSENLETKRLVRRSEAAQSQAMGKTILARYEKGCLCLLGSVVFYLLCGFLAAAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBP7</accession>
    <name>C5DBP7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0A04356p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0A04356g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551646.1">
        <property type="nucleotide sequence ID" value="XM_002551600.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBP7"/>
    <dbReference type="STRING" id="559295.C5DBP7"/>
    <dbReference type="GeneID" id="8290449"/>
    <dbReference type="KEGG" id="lth:KLTH0A04356g"/>
    <dbReference type="eggNOG" id="KOG2914">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045011_13_4_1"/>
    <dbReference type="InParanoid" id="C5DBP7"/>
    <dbReference type="OrthoDB" id="40579at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000121">
        <property type="term" value="F:sn-glycerol 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006114">
        <property type="term" value="P:glycerol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006970">
        <property type="term" value="P:response to osmotic stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07527">
        <property type="entry name" value="HAD_ScGPP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000033">
        <property type="entry name" value="Sugar phosphatase YfbT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="entry name" value="Putative phosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051806">
        <property type="entry name" value="HAD-like_SPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43481">
        <property type="entry name" value="FRUCTOSE-1-PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43481:SF4">
        <property type="entry name" value="GLYCEROL-1-PHOSPHATE PHOSPHOHYDROLASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01135">
        <property type="entry name" value="C1.5.6:_HAD__Beta-PGM__Phospha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00037">
        <property type="entry name" value="glycerol-3-phosphate_phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR21204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="249" mass="27446" checksum="F05FC5067FA72915" modified="2009-07-28" version="1">MSSSKPISLKVNAALFDVDGTIIISQPATNAFWRDFAKDKPYFIVDEVLKVSHGCRTFDTISRFAPDFADEAKIVEMETQIPAKYGEHSIEVPGAVNLCHALNTLPKEKWAVATSGTRGLAVQWFKLLGITMPTYFITANDVKQGKPHPEPYLKGRNGLGYPVNEKEPSKSKAIVFEDAPAGIAAGKAAGCKVIGIATTFDLDYLKKHDCDIIVEDLSTVRIGSYDAETDEVEFIFSDYLYAKDDVLTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBS9</accession>
    <name>C5DBS9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">U three protein 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0A05082g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in nucleolar processing of pre-18S ribosomal RNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551678.1">
        <property type="nucleotide sequence ID" value="XM_002551632.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBS9"/>
    <dbReference type="FunCoup" id="C5DBS9">
        <property type="interactions" value="1188"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBS9"/>
    <dbReference type="GeneID" id="8290482"/>
    <dbReference type="KEGG" id="lth:KLTH0A05082g"/>
    <dbReference type="eggNOG" id="KOG1272">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022996_1_1_1"/>
    <dbReference type="InParanoid" id="C5DBS9"/>
    <dbReference type="OMA" id="EFLPYHW"/>
    <dbReference type="OrthoDB" id="10251154at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000378">
        <property type="entry name" value="U3 small nucleolar RNA-associated protein 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012952">
        <property type="entry name" value="BING4_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040315">
        <property type="entry name" value="WDR46/Utp7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14085:SF3">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14085">
        <property type="entry name" value="WD-REPEAT PROTEIN BING4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08149">
        <property type="entry name" value="BING4CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01033">
        <property type="entry name" value="BING4CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="4 8" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="269"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="BING4 C-terminal" evidence="10">
        <location>
            <begin position="365"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="13"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00076453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR21236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="552" mass="61400" checksum="716F48FC66CA0A90" modified="2009-07-28" version="1">MAKSSKPATGSHGVRDNAGKFDRDASVSKRTNGYKTKDKKLRAGLKRIDDQYKDAVSSAAATEYLLPESSGFLEAEDEMEKTFRVKQDEIKSAVDVTTANKALDLKLTNFGPYSVNYSRNGTHLLVCGKKGHVASMDWRKGELRAELNLNETCQSAIYLQNEQYFAVAQKKYTFVYDHEGVELHRLKQHIEVKHMEFLPYHYLLATAGQTGWLKYQDVTTGQLQAELRTKLGPTSAMAQNPWNAIIHLGHSNGTVTLWAPNMPTPLARLLSARGPITSIAIDRQGYYMATTGADKSLKLWDIRNFRELHTIENLPTPGSNLAISDTGLLALSRGPHVTLWKDAFRSNKSSRPCFGSSGLPDRNTPYMSSMFPGNKVNNMQFVPFEDLLGVGHGTGVRNLIIPGAGEANYDALEVNPFETAKQRQEQEVRTLLNKLPADSIALDPTVIGTVDKRASATRLNAKDLAEITNAKALESKTNKDIPQVMPSVKGKNSGLRSFLRKKTQNVIDERKLRVNAQLEKEKAARQRKEMVKRGEIEEGHQDLVNEALSRFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBT4</accession>
    <name>C5DBT4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Threonine--tRNA ligase, cytoplasmic</fullName>
            <ecNumber evidence="3">6.1.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Threonyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">KLTH0A05192g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>tRNA(Thr) + L-threonine + ATP = L-threonyl-tRNA(Thr) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24624"/>
            <dbReference type="Rhea" id="RHEA-COMP:9670"/>
            <dbReference type="Rhea" id="RHEA-COMP:9704"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78534"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.3" evidence="3"/>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551683.1">
        <property type="nucleotide sequence ID" value="XM_002551637.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBT4"/>
    <dbReference type="FunCoup" id="C5DBT4">
        <property type="interactions" value="1049"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBT4"/>
    <dbReference type="GeneID" id="8290487"/>
    <dbReference type="KEGG" id="lth:KLTH0A05192g"/>
    <dbReference type="eggNOG" id="KOG1637">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008554_0_1_1"/>
    <dbReference type="InParanoid" id="C5DBT4"/>
    <dbReference type="OMA" id="WYADGMY"/>
    <dbReference type="OrthoDB" id="5423599at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004829">
        <property type="term" value="F:threonine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006435">
        <property type="term" value="P:threonyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01667">
        <property type="entry name" value="TGS_ThrRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00860">
        <property type="entry name" value="ThrRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00771">
        <property type="entry name" value="ThrRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000009">
        <property type="entry name" value="Threonine--tRNA ligase 2, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.800:FF:000003">
        <property type="entry name" value="Threonine--tRNA ligase 2, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000006">
        <property type="entry name" value="Threonine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.980.10:FF:000005">
        <property type="entry name" value="Threonyl-tRNA synthetase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.980.10">
        <property type="entry name" value="Threonyl-trna Synthetase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00184">
        <property type="entry name" value="Thr_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002320">
        <property type="entry name" value="Thr-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047246">
        <property type="entry name" value="ThrRS_anticodon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033728">
        <property type="entry name" value="ThrRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00418">
        <property type="entry name" value="thrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451:SF46">
        <property type="entry name" value="THREONINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451">
        <property type="entry name" value="THREONINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01047">
        <property type="entry name" value="TRNASYNTHTHR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="TGS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="ThrRS/AlaRS common domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TGS" evidence="15">
        <location>
            <begin position="74"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="14">
        <location>
            <begin position="321"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="14"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="14"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00073157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAR21241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="740" mass="85745" checksum="1375C6FCA26E6FBC" modified="2009-07-28" version="1">MSVKEAVNRVEEKVKDLVLDNKDNKSSEKGNGKSKNKQDSKQSSLYLDPEPDFIDERNQLFDKLLKEYQEKVEQMPRVPIKVVLKDGSEKEATAWETTPMDIAKQIAKSFPDKQCISKANGQLWDLERPLEGKEGDEVKLEFFDFDSDEGKRVFWHSSAHVLGEACECNLGAHICLGPPTEDGFFYEFAVKNAPNAPADAPERTVSQNDFPALESVSKNVIKEKQKFERLVMSKEDLLKMFHYSKFKTYLVSTKVPDGGSTTVYRCGKLIDLCVGPHIPHTGRIKAFKLLKNSSCYFLGDSNNDSLQRIYGISFPDKKLMDAHLKFLAEASMRDHRKIGREQELFYFNEMSPGSCFWLPHGTRIYNNLVDLMRAEYRKRGFDEVITPNMYNSKLWETSGHWGNYKENMFTFEVEKETFGLKPMNCPGHCILFKSRERSYKELPWRVADFGVIHRNEFSGALTGLTRVRRFQQDDAHIFCAQNQIEQEIENCFSFLQYVYGVFGFEFKMELSTRPEKFVGEIETWNNAEAKLESALRKWGGNWELNPGDGAFYGPKIDIKISDALRRWHQCATIQLDFQLPNRFELEFKSKDNQENENYERPVMIHRAILGSVERMTAILTEHFAGKWPFWLSPRQVLVVPVGVKYQEYAQEVRDKLCEQDFYADVDLTGNTLQKKVRNGQMLKYNFIFIVGEQEMTEKSVNIRNRDVIEQQGKNATIAFETVLQQLKKLKEEKRLDNVLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>C5DC63</accession>
    <name>C5DC63_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">KLTH0B00330p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0B00330g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551812.1">
        <property type="nucleotide sequence ID" value="XM_002551766.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DC63"/>
    <dbReference type="FunCoup" id="C5DC63">
        <property type="interactions" value="1256"/>
    </dbReference>
    <dbReference type="GeneID" id="8290631"/>
    <dbReference type="KEGG" id="lth:KLTH0B00330g"/>
    <dbReference type="eggNOG" id="KOG0254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_30_1_1"/>
    <dbReference type="InParanoid" id="C5DC63"/>
    <dbReference type="OMA" id="PHENDIE"/>
    <dbReference type="OrthoDB" id="5141738at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055056">
        <property type="term" value="F:D-glucose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17356">
        <property type="entry name" value="MFS_HXT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000044">
        <property type="entry name" value="Hexose transporter Hxt3p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF75">
        <property type="entry name" value="GALACTOSE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="110"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="141"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="164"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="201"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="234"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="361"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="386"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="429"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="463"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="491"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="11">
        <location>
            <begin position="65"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="11"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR21373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="566" mass="62984" checksum="1550C6B5EED8FEBF" modified="2009-07-28" version="1">MSEPGVKLDRVSSNPVEGSTKPGSISSHEKLDIEREKDLHNFPPHENDIELPKQPLSHYFSISILCLMIAFGGFIFGWDTGTISGFVNLSDFVRRFGSRNSKGVYYLSKVRMGLIISIFNIGCAIGGIVLSKIGDVYGRRIGLISVTIVYVIGIIIQISSTDKWYQYFIGRIISGLGVGGIAVLSPMLLSEVSPKHIRGMLVNLYQLLGTMGIFLGYCTNYGTKKYTDSTQWRVGLGLCFAWAMLMISGMLFVPESPRYLVEAGKLEEAKRSLSRSNKVLIDDPAIAAELDAISAGVESERLAGDATWGELLSTKTKVIQRVIMGIMIQSLQQLTGDNYFFYYGTTIFKSIGLEDSFETSIIIGAVNFFSSFIAIYTIERFGRRTCLLWGAASMFCCFIVFASVGVTKLWPEGPDHPGISSKGAGNCMIVFTMFFIFSFATTWAGCCYVIVSETFPLRVKSKGMALSTAANWMWGFLISFFTPFITGAINFYYGYVFMGCLIFAYFYVFFFVPETKGLTLEEVDIMWLESVPAWNSSSWVPPTRRSADYVVDTSSKNFFSKFFSKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCK3</accession>
    <name>C5DCK3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome b mRNA-processing protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0B03762g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Essential for the assembly of ubiquinol-cytochrome c reductase. It has a direct effect on the correct occurrence of the Rieske protein, core 4, core 5 and apocytochrome b.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Mitochondrion inner membrane</location>
            <topology evidence="1 10">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the CBP4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551952.1">
        <property type="nucleotide sequence ID" value="XM_002551906.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCK3"/>
    <dbReference type="FunCoup" id="C5DCK3">
        <property type="interactions" value="57"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCK3"/>
    <dbReference type="GeneID" id="8290786"/>
    <dbReference type="KEGG" id="lth:KLTH0B03762g"/>
    <dbReference type="eggNOG" id="ENOG502S2G8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_147520_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCK3"/>
    <dbReference type="OMA" id="KDPIWKT"/>
    <dbReference type="OrthoDB" id="5576752at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034551">
        <property type="term" value="P:mitochondrial respiratory chain complex III assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012420">
        <property type="entry name" value="Cbp4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28202">
        <property type="entry name" value="ASSEMBLY FACTOR CBP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28202:SF1">
        <property type="entry name" value="ASSEMBLY FACTOR CBP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07960">
        <property type="entry name" value="CBP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0143">Chaperone</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 10" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="102"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU368005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR21514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="143" mass="16875" checksum="AC0BA598E8453FB5" modified="2009-07-28" version="1">MDKPLWLRWLRVYIAGGAIIGVGAVLFKYTTPTDEELINSLSPELRLQYERERQLRQAEQKELMNIVRETAKSDDPIWKTGAINSPWEKNSSEQSREQFQRVKAEEIQKEELQRIRQELQQIRADSVAKTQEEVKKSAGWKFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCN6</accession>
    <name>C5DCN6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0B04554p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0B04554g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551985.1">
        <property type="nucleotide sequence ID" value="XM_002551939.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCN6"/>
    <dbReference type="FunCoup" id="C5DCN6">
        <property type="interactions" value="469"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCN6"/>
    <dbReference type="GeneID" id="8290822"/>
    <dbReference type="KEGG" id="lth:KLTH0B04554g"/>
    <dbReference type="eggNOG" id="KOG0251">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025901_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCN6"/>
    <dbReference type="OMA" id="AWTIVFK"/>
    <dbReference type="OrthoDB" id="44015at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005545">
        <property type="term" value="F:1-phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032050">
        <property type="term" value="F:clathrin heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048268">
        <property type="term" value="P:clathrin coat assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006900">
        <property type="term" value="P:vesicle budding from membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="ANTH_N_YAP180"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.150">
        <property type="entry name" value="ANTH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011417">
        <property type="entry name" value="ANTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014712">
        <property type="entry name" value="ANTH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045192">
        <property type="entry name" value="AP180-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951">
        <property type="entry name" value="CLATHRIN ASSEMBLY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951:SF5">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-BINDING CLATHRIN ASSEMBLY PROTEIN LAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07651">
        <property type="entry name" value="ANTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89009">
        <property type="entry name" value="GAT-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="2">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="260"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="410"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="527"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="265"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="410"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="479"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="543"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="558"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="576"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="612"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="654"/>
            <end position="674"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR21547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="686" mass="76956" checksum="7FCF8DF6B559C165" modified="2009-07-28" version="1">MTTYVKLVKGATKIKMAPPKAKYVDPILLGTADPHEFREIMNALDARVQDTAWTIVYKSLIVVHLMIREGEPLVTIKYLSKNQDFFSLKDIFHSKLSSGDLQALRRYRDYLRTRCVEYANTGKDYVRENNSSLTTSAASDPKLSLSHVESLEAQISALIKNRYSQYDLGNDLLLTAFRLLVQDLLVLYNSLNEGIITLLESFFELTHQDAERTLKLYKRFVELTESVVKYLKTGKAVGLKIPVIKHITTKLIRSLEEHLKDDNNNHGQNFSSSDSKTPAQRELEQIREQKRQLEEQMKTQQQMIISPTIPQQQTGYNPFAEGFSFEQPAAAQPIATQPTSNPFMAQAQVPQHTAQPLQYQQTQQAVQPLQYQQTQQTVQPLQYQQTQQTPAVGQLQQVSTTGQFQQTPMAGQFQAAQHMAQQAPQQVPQQMSQQMPQADQYQQAQMQQQTQVPQQTPQAFQYQHTQGPQQASPGEHFQPFQSLNPQTAQPSPQLQSASTGFFSSNTQVTPSFTGAGFGGYYSPDTGAGAASGPGPAPAPVQTAMPTGSNNPFSMENIARSKDERERVNPFSQTNQSPALNSSAAANPFDKPMQNHHTFGGLENMPTVSVFPQTRSQTTQLQQAQIQPQHTQLQFQQPQQAYGHPQQSSLNPYTQAQQNLQQQQQQQQQYGNLQYSRPGEGPNLIDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCP8</accession>
    <name>C5DCP8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Peroxisomal membrane protein PEX13</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Peroxin-13</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">KLTH0B04818g</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="13">Interacts (via SH3 domain) with PEX14 (via SH3-binding motif); forming the PEX13-PEX14 docking complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peroxin-13 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551997.1">
        <property type="nucleotide sequence ID" value="XM_002551951.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCP8"/>
    <dbReference type="FunCoup" id="C5DCP8">
        <property type="interactions" value="204"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCP8"/>
    <dbReference type="GeneID" id="8290834"/>
    <dbReference type="KEGG" id="lth:KLTH0B04818g"/>
    <dbReference type="eggNOG" id="KOG3875">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034386_2_0_1"/>
    <dbReference type="InParanoid" id="C5DCP8"/>
    <dbReference type="OMA" id="EGWFPKK"/>
    <dbReference type="OrthoDB" id="10037838at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990429">
        <property type="term" value="C:peroxisomal importomer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016560">
        <property type="term" value="P:protein import into peroxisome matrix, docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11771">
        <property type="entry name" value="SH3_Pex13p_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000128">
        <property type="entry name" value="Peroxisomal membrane protein (Pex13)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007223">
        <property type="entry name" value="Peroxin-13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035463">
        <property type="entry name" value="Pex13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19332">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19332:SF1">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04088">
        <property type="entry name" value="Peroxin-13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="263"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="17">
        <location>
            <begin position="308"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00065871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAR21559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="380" mass="39732" checksum="2519F5BD07931671" modified="2009-07-28" version="1">MSTSVKQPRPKPWEAQGGQNTPMDDGAMITAGEAQGTRGEGAKADGNAAPEPPQKPAALGPENGVGARSGLGNAGTFLGSGGPYSSGYGGSLYGGGMGSMYSGGYNSMYGGGYGSMYGGGYGSMMGGGYGAGGMYGMNNNGAQTLAESTQATFQLIEGLIGAVAGFAQMLEATYMATHNSFFTMVSMAEQISSLKEMVGSFFGIFAAMKMLKRLLYKVTNGRAGLAPARAAAGSKSPLVQEFAKFGDASAQAKRRRRMSWKPLLVFVAAVVGFPLLLRKFIAKLSEIQQRRLGAGAVGAGAGGTLDPRNLEFARAVYDFTPENPQVEAALRKGDLMAVISKQDPLGNASEWWQVRTKKGDVGYVPSNYVELIRRTRSESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCW3</accession>
    <name>C5DCW3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Prefoldin subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0B06292g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 4">Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Heterohexamer of two PFD-alpha type and four PFD-beta type subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the prefoldin subunit beta family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552062.1">
        <property type="nucleotide sequence ID" value="XM_002552016.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCW3"/>
    <dbReference type="FunCoup" id="C5DCW3">
        <property type="interactions" value="775"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCW3"/>
    <dbReference type="GeneID" id="8290901"/>
    <dbReference type="KEGG" id="lth:KLTH0B06292g"/>
    <dbReference type="eggNOG" id="KOG1760">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130032_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCW3"/>
    <dbReference type="OMA" id="KFGRAIN"/>
    <dbReference type="OrthoDB" id="10250441at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd23165">
        <property type="entry name" value="Prefoldin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.370:FF:000005">
        <property type="entry name" value="Prefoldin subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002777">
        <property type="entry name" value="PFD_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016661">
        <property type="entry name" value="PFDN4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21100">
        <property type="entry name" value="PREFOLDIN SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21100:SF9">
        <property type="entry name" value="PREFOLDIN SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01920">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016477">
        <property type="entry name" value="Prefoldin_subunit_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="75"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR016477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR21624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="129" mass="15077" checksum="3AE5BC976E56B24A" modified="2009-07-28" version="1">MDLLPEGKKNTVQVLYEDQQKINEFSKLIMRKDAINQELAHYKQEKGYLDDVSLEIELIDEDEQIQYRIGEAFVFMKQSEVVEQLEKDAEALDQKIEELEDADSEISSRTSELKTVLYAKFGDNINLER</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>C5DD76</accession>
    <name>C5DD76_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">EKC/KEOPS complex subunit BUD32</fullName>
            <ecNumber evidence="6">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="24 25">Atypical Serine/threonine protein kinase BUD32</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">EKC/KEOPS complex subunit bud32</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="29" type="ordered locus">KLTH0B08954g</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="29 30" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="30">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the EKC/KEOPS complex composed of at least BUD32, CGI121, GON7, KAE1 and PCC1; the whole complex dimerizes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. BUD32 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="6"/>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552175.1">
        <property type="nucleotide sequence ID" value="XM_002552129.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DD76"/>
    <dbReference type="FunCoup" id="C5DD76">
        <property type="interactions" value="929"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DD76"/>
    <dbReference type="GeneID" id="8291018"/>
    <dbReference type="KEGG" id="lth:KLTH0B08954g"/>
    <dbReference type="eggNOG" id="KOG3087">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063953_1_1_1"/>
    <dbReference type="InParanoid" id="C5DD76"/>
    <dbReference type="OMA" id="HKLYMEY"/>
    <dbReference type="OrthoDB" id="3399at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000408">
        <property type="term" value="C:EKC/KEOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070525">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000745">
        <property type="entry name" value="Serine/threonine-protein kinase BUD32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000639">
        <property type="entry name" value="Serine/threonine-protein kinase BUD32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022495">
        <property type="entry name" value="Bud32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03724">
        <property type="entry name" value="arch_bud32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12209:SF0">
        <property type="entry name" value="EKC_KEOPS COMPLEX SUBUNIT TP53RK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12209">
        <property type="entry name" value="NON-SPECIFIC SERINE/THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06293">
        <property type="entry name" value="Kdo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="21" id="KW-0010">Activator</keyword>
    <keyword evidence="18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0158">Chromosome</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="16" id="KW-0418">Kinase</keyword>
    <keyword evidence="15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="23" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="19" id="KW-0779">Telomere</keyword>
    <keyword evidence="22" id="KW-0804">Transcription</keyword>
    <keyword evidence="20" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="13" id="KW-0808">Transferase</keyword>
    <keyword evidence="14" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Protein kinase" evidence="28">
        <location>
            <begin position="16"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00019973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00030980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00033194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="EMBL" id="CAR21737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="265" mass="30236" checksum="4EF25B9CCCD88B02" modified="2009-07-28" version="1">MSKEVIEQVVTYLTPNVPIVPISQGAEAFVFTTNVHPYNASACKTAGDIPVKYIIKYRPPKKYRHPSIDKTLTKHRTLSESRILAKLSQVPGLHVPKLIACDVYNGCIWIEFIGEDLPESHGFSNLKNFLWMYKTDPYNIAVEKVLFKVGEQIGLLHWNDYCHGDLTTSNLVLHQENGDWSPYLIDFGLGSASTLVEDKGVDLYVLERAIISTHSKFADKYNEWILMGFKSVYENEGKKGQQKLKDVIKRFEEVRLRGRKRSMLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDH0</accession>
    <name>C5DDH0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0C00924p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0C00924g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552269.1">
        <property type="nucleotide sequence ID" value="XM_002552223.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDH0"/>
    <dbReference type="FunCoup" id="C5DDH0">
        <property type="interactions" value="1260"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDH0"/>
    <dbReference type="GeneID" id="8291127"/>
    <dbReference type="KEGG" id="lth:KLTH0C00924g"/>
    <dbReference type="eggNOG" id="KOG0152">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005825_1_1_1"/>
    <dbReference type="InParanoid" id="C5DDH0"/>
    <dbReference type="OMA" id="NEPIYKH"/>
    <dbReference type="OrthoDB" id="187617at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.440">
        <property type="entry name" value="FF domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002713">
        <property type="entry name" value="FF_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036517">
        <property type="entry name" value="FF_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039726">
        <property type="entry name" value="Prp40-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11864">
        <property type="entry name" value="PRE-MRNA-PROCESSING PROTEIN PRP40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11864:SF0">
        <property type="entry name" value="PRP40 PRE-MRNA PROCESSING FACTOR 40 HOMOLOG A (YEAST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01846">
        <property type="entry name" value="FF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00441">
        <property type="entry name" value="FF"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81698">
        <property type="entry name" value="FF domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51676">
        <property type="entry name" value="FF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WW" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="2">
        <location>
            <begin position="44"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="FF" evidence="3">
        <location>
            <begin position="336"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="534"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="535"/>
            <end position="548"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR21831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="564" mass="65961" checksum="7755139FB0D5747F" modified="2009-07-28" version="1">MSSWKEANDAEGRVYYYNADSGETTWDKPRELFTQLELKLEKHGWKTGKTDEGQVYYYNQETGKSCWEIPTFEEHGEEEEAERPHTSDERSTTPAQSNIESYVNNSIILNAPVMSQNEAEHVFMEMLKEHQIDSTWSFNKIISELGCKDPRYWCVDDDPLWKSQAFEKYLSNRSEDQLLKEHSAVNKFKAAFTAMLSQNKDIRYYTRWTTAKRLFANEPIYKHSVVSERTKRQVFQDYVDDLRRNQSEELNKTKQQAKTELQDYLESIMPDKKSLLSWQELSTKYLFENSTRFTSNRHFQALSKHDVLMQYITIVEAYTSKTEEELKKLKSANYTKDRIARDHFKELLAEHSKSIRCNSKWEDLYSLFKSDSRFLSLLGRNGSSALDLFMDLVEEKANIMKAQKSIANQILIDTSFQWSSDAVQDREKVNEILSKHQQLNSLDTIDRGILVDKMIDDQNQKRAQQAEMIQRLLEQRKKYFLLLLQRVFSSPNAKPETWEKARDVLKGYPEYIDIKEESVKEQIFKEFEPAKGQNTAAAAATGSSALAAPQISRKRQMTPVELDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDW0</accession>
    <name>C5DDW0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0C04224p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0C04224g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sorting nexin family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552409.1">
        <property type="nucleotide sequence ID" value="XM_002552363.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDW0"/>
    <dbReference type="FunCoup" id="C5DDW0">
        <property type="interactions" value="54"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDW0"/>
    <dbReference type="GeneID" id="8291274"/>
    <dbReference type="KEGG" id="lth:KLTH0C04224g"/>
    <dbReference type="eggNOG" id="KOG2101">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002131_1_0_1"/>
    <dbReference type="InParanoid" id="C5DDW0"/>
    <dbReference type="OMA" id="AMYVVEV"/>
    <dbReference type="OrthoDB" id="120967at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06876">
        <property type="entry name" value="PX_MDM1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003114">
        <property type="entry name" value="Phox_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013937">
        <property type="entry name" value="Sorting_nexin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22775">
        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22775:SF3">
        <property type="entry name" value="SORTING NEXIN-13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08628">
        <property type="entry name" value="Nexin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02194">
        <property type="entry name" value="PXA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00313">
        <property type="entry name" value="PXA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51207">
        <property type="entry name" value="PXA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PXA" evidence="5">
        <location>
            <begin position="84"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="RGS" evidence="3">
        <location>
            <begin position="417"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="domain" description="PX" evidence="4">
        <location>
            <begin position="763"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="639"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="643"/>
            <end position="666"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR21971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1115" mass="127171" checksum="6498322BF4AC441A" modified="2009-07-28" version="1">MAKGRLRRTITFTFYAAIFLLCFSSLCRKLLLCAAVFQLAVKIPCGYKFELVREPSPKECPRPHGSAIHTSTSRAARQPIFPNNPEVSHEVELIIDSIVRDFVASWYQSISSDPQSPFLEELKSVLYTVFINLETAVSKVDLPEFAVLKVLPLVTKHFKAFRVAREKVTSRLLLKSSPSEGDVSFAVAVEFGRYYRVHPAISLGSEPLGVSLERYSREKALFILQRTLNDAELSSKFVQILGREILCCNVICPMLQRFVNADFWNDTVESVATTILRERTQVKEIRRVLSTQLDAPKFDIERRSLSQLESVDFPMNISIETNSKGFEKFLKCINSESTEQSLRAIKFSVLSQLLNLGQAKKAGKEEQLFKSRLLLSLNLLESRLEYLRKGSSEAPVLLNELASTQIDAFKRFLSSMSIDDVLSDEFCCNVFYEFLKENNKKGECCLKFWMRIDEFKNPLEDPSTDDLAMMSEGDVKGLHDACSDFFSSSNMPVMQTLNEQYAQDISDFCKAQICSNLRTAGELYPAARKSAMRMQALAHEYLQENCLPNFKASQSFSNMISTDSFHNTRLYTNFVFGSEGGRSRQEYRKFVAFPQQEVNDLDKVKESQEGEDEALHLKVKKSFSDIFGRNEEGVIFESKIFEDESSENENDSSEDYEQSDFSEEGNEISNSRVEISWPSSSMHGNRADLKSSIAELTISIDRLRKQLSLLDHLCLKADLTDSGSQLRLLKKSERSVTKEIYQKELLRQQLIVQENSNSLFRKCQVSIKSHLSDISQDTGKEVVYYIISVSHSNNDVITSWDTPRRYSEFYELNAYLRKRFSGLVKHLQNKDYFPEKIKMSLVYHVSKTFLYKERSVKLEKYLRNLLLISEVCQDQHFRRFLTQAGSTFRIKSTSKINEDRHSLLSRIDSVSSKMCDAKIASTASPNHVKSPELELKLEDEMFKVPGQGGAETDQIKKGNFIKPICDLFITLFSLNHSRSSWLRGRALLIVIQQLLGGTIEKYVKESIERISEKDNILKTVSKLRLTLWPDGVFYKKTQTQERPQKGGDELLASQKDAKAKLEMLLSETCGRVVGIRSSKKAGAEINAMFQNEYLNASFFLEVLDLVFAEAFANFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>C5DE08</accession>
    <name>C5DE08_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Chromatin modification-related protein EAF6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0C05346g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of selected genes principally by acetylation of nucleosomal histone H4 and H2A. The NuA4 complex is also involved in DNA repair.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the NuA4 histone acetyltransferase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the EAF6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552457.1">
        <property type="nucleotide sequence ID" value="XM_002552411.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DE08"/>
    <dbReference type="FunCoup" id="C5DE08">
        <property type="interactions" value="129"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DE08"/>
    <dbReference type="GeneID" id="8291324"/>
    <dbReference type="KEGG" id="lth:KLTH0C05346g"/>
    <dbReference type="eggNOG" id="KOG3856">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093901_2_1_1"/>
    <dbReference type="InParanoid" id="C5DE08"/>
    <dbReference type="OMA" id="FVKQQEG"/>
    <dbReference type="OrthoDB" id="440324at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015418">
        <property type="entry name" value="Eaf6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13476">
        <property type="entry name" value="CHROMATIN MODIFICATION-RELATED PROTEIN MEAF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09340">
        <property type="entry name" value="NuA4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="6 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU368022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR22019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="102" mass="11621" checksum="8DA250CCAAD04397" modified="2009-07-28" version="1">MDEAHQHYQKLKQELQEALVDRETLEKQWDQIQQEIFDKESAYLTGNSSSQYGTIVKGFDAFGKHIHDSAHAFSDKDRIFSLSSARFVKQQEGDDKGGDGQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEB0</accession>
    <name>C5DEB0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0C07722p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0C07722g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552559.1">
        <property type="nucleotide sequence ID" value="XM_002552513.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEB0"/>
    <dbReference type="GeneID" id="8291431"/>
    <dbReference type="KEGG" id="lth:KLTH0C07722g"/>
    <dbReference type="eggNOG" id="ENOG502S4KN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056726_0_0_1"/>
    <dbReference type="InParanoid" id="C5DEB0"/>
    <dbReference type="OMA" id="GMLQFFI"/>
    <dbReference type="OrthoDB" id="4030310at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.320.10">
        <property type="entry name" value="YwqG-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015315">
        <property type="entry name" value="DUF1963"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035948">
        <property type="entry name" value="YwqG-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36436">
        <property type="entry name" value="SLL5081 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36436:SF6">
        <property type="entry name" value="SLL5081 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09234">
        <property type="entry name" value="DUF1963"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103032">
        <property type="entry name" value="Hypothetical protein YwqG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR22121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="267" mass="30929" checksum="0B7C46FA843A886C" modified="2009-07-28" version="1">MIATLGPNMKENFELPEYFEKHRAVLKDTIKECVDIHAEKASTGTLDSKFGGCPYFPKGMAYPLNQEGNPLKLLAQINFEQVPRLNNYPEKGILQFFLDPCDELYGCSFDENTEQKNFRIIYHEEIETDESKLLSDFSFVKVEEEMFPIESEAKLIFTKGTQIMPAGDFRFDSLIGADDKYEHYAELERYYELYGSLSHKIGGYPGFTQFDPREHYHKDRTELLLQVDSDDAIDIIWGDCGVANFFISEEDLKNKDFSKVLYNWDCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEF7</accession>
    <name>C5DEF7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0C08800p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0C08800g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552606.1">
        <property type="nucleotide sequence ID" value="XM_002552560.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEF7"/>
    <dbReference type="FunCoup" id="C5DEF7">
        <property type="interactions" value="185"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEF7"/>
    <dbReference type="GeneID" id="8291480"/>
    <dbReference type="KEGG" id="lth:KLTH0C08800g"/>
    <dbReference type="eggNOG" id="ENOG502QU8E">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_314960_0_0_1"/>
    <dbReference type="InParanoid" id="C5DEF7"/>
    <dbReference type="OMA" id="NMEGGLY"/>
    <dbReference type="OrthoDB" id="5368598at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023041">
        <property type="entry name" value="Glucose_rcpt_Git3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022596">
        <property type="entry name" value="GPR1/2/3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23112">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR 157-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23112:SF37">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR GPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11710">
        <property type="entry name" value="Git3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11970">
        <property type="entry name" value="GPR_Gpa2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="87"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="131"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="177"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="252"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="591"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="630"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose receptor Git3-like N-terminal" evidence="7">
        <location>
            <begin position="50"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="G protein-coupled receptor GPR1/2/3 C-terminal" evidence="8">
        <location>
            <begin position="581"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="853"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="853"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="921"/>
            <end position="930"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF11710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF11970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR22168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="950" mass="108245" checksum="45827B47374FD9CD" modified="2009-07-28" version="1">MPQPQNSTFLVNGEVVPPVYLNRTWNTTTVNQLLGLPGMFSTFTAPELLRLRGIAITSSSISILAAAVGFLYITGIDQRRRVFRHNLVFFLIVCDFLKALVLLIYPVVILARNDVYGMPAFYNTLGWLSAFAIEGADFAIAIFAIHFALLIFVPSWKWQNRSTGNMEGGLYRMRHSLYPVTFLVPMILASLAFVGFNTFIPVNLNDRVVLDNDSFDFSYQARKGGYKALSAWCYLPPHPVWYRICLSWGPRYFILVLIMFIYVSIYIFVSKENRKIKNQIGSFKSKDSLDIHRARDSMGKILTLQQRLLIYLKFFYRKSRIPKIMSALGNFFFLSLEDYRHEDLTERESSDQALASLRRANGDDVTARAASDLNSNYIPLSLARRNVLSSLKASNKRFSCSCPTLPRYRLQRRGGSVGNLRTACSSAGSTRRHSVNVSAFGDSVSTTNVFEPPHQLSNEEPNWNKALGVPSRVGSKNSQSGLTAYPKTNMTSSSLQATNSFERSFNFERQQNSTNLSPVRSVASNRDSSFRTAPVHLKNTTSRTLTRLNADPDDPDVIALDTNHLNGVKQNFQSETYQEFKKRRAQIQKQMRAIFIYPFSYILIWTFPLAVDVIQYRYEIVHGPVVWLEYIATFMQPLSCFVDVMVFIYRERPSRHSWASITKNELLNTYSLKGELGEQKIRELCNSELGKKGWYYRGRWTKLGCWRYKPQRWKRVAWFIYRFVKGFVKNNYDFSDNCYDATYWEAHYTGKSPSENMGSSNMRCSEALKEILDVNNTRKDSYFSGSTSEYLYENSGFVKVPLKWRILHCLPMLEGVDLDELEYQLRTSNNSGNFEIPGLNLAVNNFGKNKNEDNSNSFFKPDYNMTNTSAGGPKSPSARPQPVTAKHSSSSSSSPLHSLIYEGSGGRRRFSSAIFGDDSRNNFTNSSQDQDINEEDSSGKQLGLMDFLKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEL2</accession>
    <name>C5DEL2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0C10164p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0C10164g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552661.1">
        <property type="nucleotide sequence ID" value="XM_002552615.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEL2"/>
    <dbReference type="STRING" id="559295.C5DEL2"/>
    <dbReference type="GeneID" id="8291541"/>
    <dbReference type="KEGG" id="lth:KLTH0C10164g"/>
    <dbReference type="eggNOG" id="KOG3699">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006033_1_2_1"/>
    <dbReference type="InParanoid" id="C5DEL2"/>
    <dbReference type="OMA" id="NPWGLWW"/>
    <dbReference type="OrthoDB" id="3238794at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.225.10:FF:000009">
        <property type="entry name" value="Class II aldolase/adducin N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="entry name" value="Class II aldolase/adducin N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051017">
        <property type="entry name" value="Aldolase-II_Adducin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001303">
        <property type="entry name" value="Aldolase_II/adducin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004855">
        <property type="entry name" value="PRK06208.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10672">
        <property type="entry name" value="ADDUCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10672:SF25">
        <property type="entry name" value="MEIOTICALLY UP-REGULATED GENE 14 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00596">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01007">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="AraD/HMP-PK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Class II aldolase/adducin N-terminal" evidence="2">
        <location>
            <begin position="65"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR22223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="301" mass="33701" checksum="BF00DBD78B3CE8C0" modified="2009-07-28" version="1">MSTTTTATTEESAGTQFRETLKASAAPPSVEATQGVHNLSMGGLEYKNKRPTFSSKLEERQHVLKHMACAFRVFGRKGYNEGTAGHMSVRDPVDPSTFWINPLGQHFSLMRVSDLVHVDSNGNVLPDGNQAVINKAGFKIHSHLHKARPNVNAACHTHSEYGRAWSAFGREIEMLNQDSCMFYKKHTVYRNFGGVVLEDEEGERLAQALGDNKAIILQNHGLLTVGETVDEAAYLFTLLERTCKVQLQVEMASRGGLERNIIGDEEAYYTYFNTSDPETLYTEFQPEYDLERELTNAAFEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEM1</accession>
    <name>C5DEM1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0C10362p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0C10362g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552670.1">
        <property type="nucleotide sequence ID" value="XM_002552624.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEM1"/>
    <dbReference type="FunCoup" id="C5DEM1">
        <property type="interactions" value="597"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEM1"/>
    <dbReference type="GeneID" id="8291550"/>
    <dbReference type="KEGG" id="lth:KLTH0C10362g"/>
    <dbReference type="HOGENOM" id="CLU_184661_2_0_1"/>
    <dbReference type="InParanoid" id="C5DEM1"/>
    <dbReference type="OMA" id="KKGPMNT"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015157">
        <property type="entry name" value="TMA7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28632">
        <property type="entry name" value="TRANSLATION MACHINERY-ASSOCIATED PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09072">
        <property type="entry name" value="TMA7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="64" mass="6778" checksum="7EAB97561A0A7104" modified="2009-07-28" version="1">MSGRQGGKLKPLKSKKKGPQELDPEEAAFKEKQKADAAAKKALMNNLKGGKNLVSGGIKKSGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEN0</accession>
    <name>C5DEN0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0C10582p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0C10582g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552679.1">
        <property type="nucleotide sequence ID" value="XM_002552633.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEN0"/>
    <dbReference type="FunCoup" id="C5DEN0">
        <property type="interactions" value="94"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEN0"/>
    <dbReference type="GeneID" id="8291560"/>
    <dbReference type="KEGG" id="lth:KLTH0C10582g"/>
    <dbReference type="eggNOG" id="ENOG502R1IK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034126_0_0_1"/>
    <dbReference type="InParanoid" id="C5DEN0"/>
    <dbReference type="OMA" id="EVWFIYA"/>
    <dbReference type="OrthoDB" id="2159786at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070860">
        <property type="term" value="C:RNA polymerase I core factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001164">
        <property type="term" value="F:RNA polymerase I core promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001181">
        <property type="term" value="F:RNA polymerase I general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042790">
        <property type="term" value="P:nucleolar large rRNA transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053029">
        <property type="entry name" value="RNA_pol_I-specific_init_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007224">
        <property type="entry name" value="TIF_Rrn11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28244">
        <property type="entry name" value="RNA POLYMERASE I-SPECIFIC TRANSCRIPTION INITIATION FACTOR RRN11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28244:SF1">
        <property type="entry name" value="RNA POLYMERASE I-SPECIFIC TRANSCRIPTION INITIATION FACTOR RRN11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04090">
        <property type="entry name" value="Rrn11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="481" mass="54877" checksum="540AF7B8ADDBD333" modified="2009-07-28" version="1">MFELPLVRNTKRVRTVRKLRYQYINTLYQEYRKVQSFANANSTLPTPENSAAEEASDGDHNSADATSRRRRRKRRLLSVLGHAETDTELSDMEAEEGSHEADQADSEKEFFTRHEKPQETFEVWNTDRQKAVPMNTATLSYDTCKQIEKHAQKRVSAGMAHISKNANFHFQAMKDGYETVAETPESHHLVHIAQLNELLHTNIMKGKWDTAYRCFSLLIRLPGIDIRSVWGPGARILRELASNDKGLGTSEEFLGWLSGIFSSRSNFNQTMNFLMDPVFRCGSKTHAAKFVVAWLWELLFASCPDGTEHPSAEDASNRKLSHLLERLSEMVLIPPYMEDPEVWFIFAVCHLVSADQLSQRFISTKLRTSELERDISRNQVTQHITNAHMCIRTCEAKKEEFSFPRRIIEEQLACFEKRLYQNSGESTASDLGSGFELDNSPENLDTQNVLGPGMSPMIDEEENFFGSAEQVHFGFDSDSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEY3</accession>
    <name>C5DEY3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0D10714g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553176.1">
        <property type="nucleotide sequence ID" value="XM_002553130.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEY3"/>
    <dbReference type="FunCoup" id="C5DEY3">
        <property type="interactions" value="397"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEY3"/>
    <dbReference type="GeneID" id="8295415"/>
    <dbReference type="KEGG" id="lth:KLTH0D10714g"/>
    <dbReference type="eggNOG" id="KOG0583">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002888_1_1_1"/>
    <dbReference type="InParanoid" id="C5DEY3"/>
    <dbReference type="OMA" id="YRYITRT"/>
    <dbReference type="OrthoDB" id="193931at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF110">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="40"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="328"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="536"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="663"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="14"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="346"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="370"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="551"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="694"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="712"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="722"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="776"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="794"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="821"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR22738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="872" mass="95938" checksum="9077A727564B24B2" modified="2009-07-28" version="1">MTSVPQRHTYYGAPTSSYTQSRQSASYSSQKRKHVTFGPYIVGSTLGEGEFGKVKMGWSKTSSSSMDVPKQVAIKLIRRDTIPKNSEKEVKIYREINALKHLNHPNIVRLEEVLQNSKYIGIVLEYASGGEFYKYIQKKRRLKESAACRLFAQLISGVYYMHSKGLVHRDLKLENLLLDKNENLLITDFGFVNEFLPENELMKTSCGSPCYAAPELVVTARPYEARKADIWSCGVIMYAMLAGYLPWDDDPENPDGDDIGKLYRYITRTPLKFPDYIAPVPRDLLRAILVADPKKRITIAQTLHHQWLQPHLPFLSVMPEEWDHVSKNQELLRQPPQKGEAVNSARPRSNCSVSSTNSKGEKRNSLIMDSSLYSQPVPPQQSQSHAVAMPSSPSPRLRQSPVKSNNRHSRSNSAASIALQAVVDAEREYHATHPPFTSSASTSALQFEVSPHSTSPRLYTFSPTKANQNTVTSLRDSVIIETSPQKGSGAFLIASSLKESPNGTGLPEASSVPTTSGFKAPLAVKAQVPHHGTHNLALHRRPRPTSYHPASCSTFGDTSQAPTSQPAVSGEATTGHTQANIGKSTSQGIMHRDSFSMNKPFVDMQIVAGDLIKSGGDGDTVAKKIHDQVEDADHRVDLSRQRRQSQRYSVVYDKLFGVAQQSEDNAVEDAGPGGSTVERGASGTSVKRDKKRFSIMSIYSPSRSSVDSGSPKNEDKHKRTGSENNPSGSSKRTPSRQESLVNDQADTSRKSSAAKKPEPTKQASTLKEVKADASRKTSNPISKQESLGQSSVHGDGKAEARAEPEANKKNPTVQRSASLKKAAKPTQTRKKVNRSSVMVSSVAAGSTENARPREQSTAKRVIDFFKRRSMRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5DF35</accession>
    <name>C5DF35_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0D11902p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0D11902g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553228.1">
        <property type="nucleotide sequence ID" value="XM_002553182.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DF35"/>
    <dbReference type="FunCoup" id="C5DF35">
        <property type="interactions" value="119"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DF35"/>
    <dbReference type="GeneID" id="8295468"/>
    <dbReference type="KEGG" id="lth:KLTH0D11902g"/>
    <dbReference type="eggNOG" id="KOG4568">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018256_0_0_1"/>
    <dbReference type="InParanoid" id="C5DF35"/>
    <dbReference type="OMA" id="LWHERDH"/>
    <dbReference type="OrthoDB" id="2130750at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.190">
        <property type="entry name" value="CAP Gly-rich-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036859">
        <property type="entry name" value="CAP-Gly_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000938">
        <property type="entry name" value="CAP-Gly_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18916">
        <property type="entry name" value="DYNACTIN 1-RELATED MICROTUBULE-BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18916:SF93">
        <property type="entry name" value="RESTIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01302">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01052">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74924">
        <property type="entry name" value="Cap-Gly domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00845">
        <property type="entry name" value="CAP_GLY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50245">
        <property type="entry name" value="CAP_GLY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CAP-Gly" evidence="2">
        <location>
            <begin position="21"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="107"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="183"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="245"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="619"/>
            <end position="691"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR22790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="819" mass="92965" checksum="2C93C8EC35A1EE65" modified="2009-07-28" version="1">MAELGKRVALNGLQGTVRFIGETEFAPGTWCGVELDEKCGKNDGSVQGVRYFNQTLKDGLYGLFGRIETVRPISTSGSVASSPNESRNLHGVILKLQDKLYGLHHRYADLELQLENCAIDKEALKEENEELMSVIIGLQQELATLRQDLSALQQEVDLRRSIEEHEWETDAGKLSHEALWKRNQLVEVALSKLQESLDESNEECISLAAENDQIKMELSVTAAELDECKHVVESLASKSADNQIIDKLTEENTDLNARVESFKATVEELKSEHVVAKDLQQIYSDLEEELSEQLRILRENADRDKHTITLLESERAELLHELERVKSHANTRDFCQQIEQLSSQLSKTQMIAKRSVLNEKFSNIGLGDQGSRAPLTLSKQVQFLSNHLLQEQFPADLQVKLQGSFFLQLMAQELMAWHRQREIYTDTLHSFKPTAFQINEWKELIFDNKIPQECSADSIAHHLKNQGGPLNKDASFLLSFISATAETFLPLIFDILSKDFDLKKVSLIHSKAKQLATTCKKQQEQIPIGSAGVTHDPKISELLSTYFQDVVFILTSGKSDNDSIDRAIESLEALNFVLENMKFDKISSASKVSVEQPKLQSNLLVEDRVHNSNGSTPKARLRDNRIEELELKLKVYENKVERHKHQEETIVKLQQELHGLKCNKNTLEASVSALQARSSELTEKLESERIRQNFLFPKPQMIDLVKEMEATDKIDLISQISDLRRVIVKHEEAKYTLEEDKWLRKPIEARGNIVNSEFFTKLNDAHKSIDCLVKSLDVVPLDVNYSGKERSTNISSYYCKSLKERSIGIESLLSGLCKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFE7</accession>
    <name>C5DFE7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 11">Cytochrome c oxidase subunit 8, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Cytochrome c oxidase polypeptide VIII</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0D14498g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the cytochrome c oxidase (complex IV, CIV), a multisubunit enzyme composed of a catalytic core of 3 subunits and several supernumerary subunits. The complex exists as a monomer or a dimer and forms supercomplexes (SCs) in the inner mitochondrial membrane with ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Mitochondrion inner membrane</location>
            <topology evidence="1 11">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the cytochrome c oxidase VIIc family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553340.1">
        <property type="nucleotide sequence ID" value="XM_002553294.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFE7"/>
    <dbReference type="FunCoup" id="C5DFE7">
        <property type="interactions" value="123"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFE7"/>
    <dbReference type="GeneID" id="8295584"/>
    <dbReference type="KEGG" id="lth:KLTH0D14498g"/>
    <dbReference type="eggNOG" id="KOG4527">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_169812_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFE7"/>
    <dbReference type="OMA" id="IHNRKIP"/>
    <dbReference type="OrthoDB" id="9974841at2759"/>
    <dbReference type="UniPathway" id="UPA00705"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.49.10:FF:000001">
        <property type="entry name" value="Cytochrome c oxidase subunit 7C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.49.10">
        <property type="entry name" value="Cytochrome c oxidase subunit VIIc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004202">
        <property type="entry name" value="COX7C/Cox8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036636">
        <property type="entry name" value="COX7C/Cox8_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13313:SF0">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 7C, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13313">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT VIIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02935">
        <property type="entry name" value="COX7C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81427">
        <property type="entry name" value="Mitochondrial cytochrome c oxidase subunit VIIc (aka VIIIa)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 11" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="43"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00071004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR22902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="74" mass="8279" checksum="D7C5EC578555C47F" modified="2009-07-28" version="1">MIFGATSRIAARRAFTVSARQSGAHFKEGVYSNIPVKVRNRRIPFAVIHFGYFTLGFLVPFISSYVQLKKSGVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFG7</accession>
    <name>C5DFG7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0D14960p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0D14960g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553360.1">
        <property type="nucleotide sequence ID" value="XM_002553314.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFG7"/>
    <dbReference type="FunCoup" id="C5DFG7">
        <property type="interactions" value="1236"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFG7"/>
    <dbReference type="GeneID" id="8295604"/>
    <dbReference type="KEGG" id="lth:KLTH0D14960g"/>
    <dbReference type="eggNOG" id="KOG1539">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002774_2_0_1"/>
    <dbReference type="InParanoid" id="C5DFG7"/>
    <dbReference type="OMA" id="CIYAWRA"/>
    <dbReference type="OrthoDB" id="10250769at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034388">
        <property type="term" value="C:Pwp2p-containing subcomplex of 90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000410">
        <property type="entry name" value="U3 small nucleolar RNA-associated protein 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000710">
        <property type="entry name" value="U3 snoRNP protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059157">
        <property type="entry name" value="WDR36-Utp21_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007319">
        <property type="entry name" value="WDR36/Utp21_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22840">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22840:SF12">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25171">
        <property type="entry name" value="Beta-prop_WDR36-Utp21_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25168">
        <property type="entry name" value="Beta-prop_WDR36-Utp21_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04192">
        <property type="entry name" value="Utp21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="WDR36/Utp21 N-terminal" evidence="5">
        <location>
            <begin position="52"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="582"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="domain" description="WDR36/Utp21 C-terminal" evidence="4">
        <location>
            <begin position="715"/>
            <end position="936"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR22922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="939" mass="103783" checksum="42D1DDE4AF7E6E3E" modified="2009-07-28" version="1">MTESYKKRKVPSKEDGARSFAKSKVLSPFRVVGNVSNGVPFAVGTLGSTFYIVTSVGKTFQIYDANNLNLLFVSETETESVITCLAAHFQYVFVGFGNKVGIYRRGKLERLLQLPDTGVTVTDICVFGEFLCVSANNNCVYIFKKAPEDKFATIFYTKFSVSQLQGSEIVSIIHLPTYLNKIVVVTKSNVLVYNVKSGKLLFVSDEFPHQITAAESAPVLDVVALGCASGEVILFNVKKARKIRTIKTPVRVSSLSFRTDGSAHLAVGASNGDLIFYDLDRRCRIHVLKNVHKENAGGISRACFLNGQPIVVTSGNDNQLKEYVFDPSLSQGDSEVVVQPPRFLRSRGGHSKPPSSILFADNQSHFILSASSDRSFWGFSLRKDAQSQELSQRQHKKKDGGRLAGSTMKEKFSEVLAMAIENSRQGEWENVLTAHKDEKFARTWDLATKRVGRWTLNTIDDGLAKSVAISPCGNFGFVGSSNGGIGVYNLQSGLPRKKYRLHKKSVTGIAVDGMNRKMVSCGLDGIVGFYDFSKSSFLGKLQLSAPITSMVYHRSSDLFALALDDFSIVVVDAVTQKVVRQLWGHSNRVSSFDFSPDGRWIVSTSLDSTIRTWDLPTGSCIDGVRLDNVATNIRFSPNADFLATTHVSGNGILIWTNRAQFRAFPARQIDEEEFAGISTSVSFGQNGASMLDGAFDAKESNDMGEVDFGHYASVDQVSSDLITLSLGPRNKLQTLLNLDVIRQRSKPIEPPKKPEKAPFFLQLTGEKVGDDTSVRENVATQDASELEKQRGVQNKQAEAEDKLMKHKPIVASFESQFTKLLREGSLDKSYSQFLDQLINLSPASVDLEIRSLNSFEPFHEIIWFLEALTEGLLSNKNFELYEAFMSLLMKAHGDVIHTNNQNEGLSAALRNWQEVHALKDRLDDIIKYCSSVANFVSTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFL2</accession>
    <name>C5DFL2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Ornithine aminotransferase</fullName>
            <ecNumber evidence="4 9">2.6.1.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0D16038g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 2-oxocarboxylate + L-ornithine = L-glutamate 5-semialdehyde + an L-alpha-amino acid</text>
            <dbReference type="Rhea" id="RHEA:13877"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:58066"/>
            <dbReference type="ChEBI" id="CHEBI:59869"/>
            <dbReference type="EC" id="2.6.1.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-ornithine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.13" evidence="4 9"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553405.1">
        <property type="nucleotide sequence ID" value="XM_002553359.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFL2"/>
    <dbReference type="FunCoup" id="C5DFL2">
        <property type="interactions" value="898"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFL2"/>
    <dbReference type="GeneID" id="8295652"/>
    <dbReference type="KEGG" id="lth:KLTH0D16038g"/>
    <dbReference type="eggNOG" id="KOG1402">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016922_10_3_1"/>
    <dbReference type="InParanoid" id="C5DFL2"/>
    <dbReference type="OMA" id="RSAWDLC"/>
    <dbReference type="OrthoDB" id="10261433at2759"/>
    <dbReference type="UniPathway" id="UPA00098">
        <property type="reaction ID" value="UER00358"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004587">
        <property type="term" value="F:ornithine aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019544">
        <property type="term" value="P:L-arginine catabolic process to L-glutamate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010121">
        <property type="term" value="P:L-arginine catabolic process to proline via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000011">
        <property type="entry name" value="Ornithine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000152">
        <property type="entry name" value="Ornithine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049704">
        <property type="entry name" value="Aminotrans_3_PPA_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050103">
        <property type="entry name" value="Class-III_PLP-dep_AT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010164">
        <property type="entry name" value="Orn_aminotrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01885">
        <property type="entry name" value="Orn_aminotrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986">
        <property type="entry name" value="AMINOTRANSFERASE CLASS III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986:SF18">
        <property type="entry name" value="ORNITHINE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="7 8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR22967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="434" mass="47528" checksum="0B039AD6DA6CF676" modified="2009-07-28" version="1">MASVNVNLSSKQTVELEQEYSAHNYHPLPVVFHKALGAHVWDPEGKEYLDFLSAYSAVNQGHCHPEIIAALVDQASKLTLSSRAFSNDVFAAFAKYVTEYFNYEMMLPMNTGAEAVETALKLARRWGYMKKGIPDNQAIILGAHSNFHGRTFGAISLSTDEDSRNKFGPFLENTTSKIPGTTDRYLRYGVVEDVELAFQNAGDCIAALIVEPIQGEAGIVVPRADYLTKVQQLCRKYNVLLICDEIQTGIARTGKMLCYEHSPDVKPDIILLGKAISGGVLPVSCVLSSKDIMLCFEPGSHGSTYGGNPLASRVAIAALEVVRKENLVQRAQDLGEFLQGELEKLRDSSNGIISEVRGKGLLTAIVIDPKRANGRSAWDLCLLMKDQGVLAKPTHEHIIRLAPPLVISKEDLLKGVDAIRKSLELLPTVEKAAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFR8</accession>
    <name>C5DFR8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0D17380p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0D17380g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR23023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553461.1">
        <property type="nucleotide sequence ID" value="XM_002553415.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFR8"/>
    <dbReference type="FunCoup" id="C5DFR8">
        <property type="interactions" value="256"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFR8"/>
    <dbReference type="GeneID" id="8295711"/>
    <dbReference type="KEGG" id="lth:KLTH0D17380g"/>
    <dbReference type="eggNOG" id="KOG4129">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019358_1_0_1"/>
    <dbReference type="InParanoid" id="C5DFR8"/>
    <dbReference type="OMA" id="TMTIFFN"/>
    <dbReference type="OrthoDB" id="374045at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004309">
        <property type="term" value="F:exopolyphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.20">
        <property type="entry name" value="DHHA2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1640.10">
        <property type="entry name" value="inorganic pyrophosphatase (n-terminal core)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001667">
        <property type="entry name" value="DDH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004097">
        <property type="entry name" value="DHHA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038222">
        <property type="entry name" value="DHHA2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12112">
        <property type="entry name" value="BNIP - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12112:SF39">
        <property type="entry name" value="EG:152A3.5 PROTEIN (FBGN0003116_PN PROTEIN)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01368">
        <property type="entry name" value="DHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02833">
        <property type="entry name" value="DHHA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01131">
        <property type="entry name" value="DHHA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="DHH phosphoesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DHHA2" evidence="5">
        <location>
            <begin position="223"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR23023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="380" mass="43023" checksum="94D0CBA3DA6C0934" modified="2009-07-28" version="1">MGEGVLAFLKFLKTSHLVGLQEKSCLRIVCGNESADLDSVVCAITYAYLSYIKDPSKPVLPVVNIMKQDLELRRDIVWVLQQRNIPHSLLYFQEDLRELKRKLNCKVDAILVDHNDQQSVARKLVDSVIGIIDHHEDLGLHRQIKDGPRIITKAGSCSSLVSNYWFDTQKIKQNNVGKDAALLSVGALLTDTSNMSHRVERPDLVACERYKAELDDFDFDKYFQSIRQAKEDITGLLLRDVLRKDYKEFEFLSSQKKSIRCGMASVVKPLGWIIEQYSADGFATACNNFLDERSLDILLILTSWTEEGEFRRELAFYAKTSENIQISLKVASKVQGSLSLEALPAKMALADSKFLFYNQLNTNASRKQVVPHTQAAIQSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFV6</accession>
    <name>C5DFV6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0D00220p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0D00220g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552736.1">
        <property type="nucleotide sequence ID" value="XM_002552690.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFV6"/>
    <dbReference type="FunCoup" id="C5DFV6">
        <property type="interactions" value="33"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFV6"/>
    <dbReference type="GeneID" id="8294952"/>
    <dbReference type="KEGG" id="lth:KLTH0D00220g"/>
    <dbReference type="eggNOG" id="ENOG502RZMY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082825_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFV6"/>
    <dbReference type="OMA" id="THYGFIR"/>
    <dbReference type="OrthoDB" id="42525at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009784">
        <property type="entry name" value="DUF1349"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015987">
        <property type="entry name" value="UCP022704"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35332">
        <property type="entry name" value="REGULATION OF ENOLASE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35332:SF2">
        <property type="entry name" value="REGULATION OF ENOLASE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07081">
        <property type="entry name" value="DUF1349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF022704">
        <property type="entry name" value="UCP022704"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR22298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="195" mass="22078" checksum="033E6F1319BFDD03" modified="2009-07-28" version="1">MTDSPWSKGSWLNAPSQVTIDNDRLKITTDEKTDFWRQTFYGFTRDSGHFFGIKTGSSFTAELRVQGSYESLYDQAGIMVRIDSKNWIKAGIEVSDGQKMLSSVLTTGVSDWTTAVYNGNAYDFWLRVTVENGVLRLQVSSDKKRWPLVRLAPFPSSEEYFVGPMACTPKRGGLNVTFSDWKLTPPLKRELHDLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFV8</accession>
    <name>C5DFV8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0D00264p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0D00264g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the purine-cytosine permease (2.A.39) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552738.1">
        <property type="nucleotide sequence ID" value="XM_002552692.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFV8"/>
    <dbReference type="FunCoup" id="C5DFV8">
        <property type="interactions" value="77"/>
    </dbReference>
    <dbReference type="GeneID" id="8294954"/>
    <dbReference type="KEGG" id="lth:KLTH0D00264g"/>
    <dbReference type="eggNOG" id="ENOG502QQ8Y">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026016_2_2_1"/>
    <dbReference type="InParanoid" id="C5DFV8"/>
    <dbReference type="OMA" id="TIACVGW"/>
    <dbReference type="OrthoDB" id="2116389at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015205">
        <property type="term" value="F:nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015856">
        <property type="term" value="P:cytosine transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.4160.10:FF:000002">
        <property type="entry name" value="Purine-cytosine permease fcyB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4160.10">
        <property type="entry name" value="Hydantoin permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001248">
        <property type="entry name" value="Pur-cyt_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026030">
        <property type="entry name" value="Pur-cyt_permease_Fcy2/21/22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31806">
        <property type="entry name" value="PURINE-CYTOSINE PERMEASE FCY2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31806:SF1">
        <property type="entry name" value="PURINE-CYTOSINE PERMEASE FCY2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02133">
        <property type="entry name" value="Transp_cyt_pur"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002744">
        <property type="entry name" value="Pur-cyt_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="75"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="103"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="143"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="183"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="211"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="241"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="282"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="326"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="379"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="410"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="449"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR002744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR22300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="515" mass="56224" checksum="0E42F35AA26A8F49" modified="2009-07-28" version="1">MPLKSDVEVQTSDRLTETTSVLPQDDHVNKGAASCNRYFAFLDAINAALGTETKGIEPTTEDERNDASLLNASTMWFSANMVIAAFSVGVLGPIVFELNFWGCVVAVVVFAFLGVLPVAFYSVFGAELGLRQMVLSRYLLGNLTARIFAMINIIACIGWGAVNTIASAQLLHMVNPHGARCPPWAGCLIIVLSTIIVSFFGYRVIHIYEKWAWVPNLAVFLIVIARVRINGSFTIGHWEEGANTAAGILSLGGIVFGFATGWTTYASDYTVYMPSTINKYKVFFAVTAGLMLPLCFTILLGAAAGRAAMNNELYRYYYDKESTGGLLYAILVPKSLGGFGQFCCVVLAMSTVANCIPNMYSIALGTQSLWQPLAKVPRVFWTVTGNFVTLAIAIPAYYKFASFMTTFMDAIGYYLSIYIGISISEHFVYRRGRFQNYIVADWSSWDRLPVGYAGCAALFVGGVGVALGMCQSYWSGEIGRLIGDYGGDIGFELGLGFSVITYNVLRPLELKFVGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFV9</accession>
    <name>C5DFV9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0D00308p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0D00308g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552739.1">
        <property type="nucleotide sequence ID" value="XM_002552693.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFV9"/>
    <dbReference type="FunCoup" id="C5DFV9">
        <property type="interactions" value="43"/>
    </dbReference>
    <dbReference type="GeneID" id="8294956"/>
    <dbReference type="KEGG" id="lth:KLTH0D00308g"/>
    <dbReference type="eggNOG" id="ENOG502RTN8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048223_1_0_1"/>
    <dbReference type="InParanoid" id="C5DFV9"/>
    <dbReference type="OMA" id="CNATQRR"/>
    <dbReference type="OrthoDB" id="4689212at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11307">
        <property type="entry name" value="M35_Asp_f2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029482">
        <property type="entry name" value="HRXXH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039124">
        <property type="entry name" value="PRA1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39399">
        <property type="entry name" value="PROTEIN ZPS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39399:SF1">
        <property type="entry name" value="PROTEIN ZPS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13933">
        <property type="entry name" value="HRXXH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950364" evidence="1">
        <location>
            <begin position="23"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Putative peptidase" evidence="2">
        <location>
            <begin position="13"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR22301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="252" mass="28216" checksum="DBF6AC0D32AF9BBD" modified="2009-07-28" version="1">MFENFKVMKITNLLSCTSLLAAISLSAPVEYSSNATISSYVEYNSDIPGWARPTFPELYQTCNSTKRRILQSFIEDSLEVSAYAKDRLLNYGAEDVFFQRWFGNGSIITVLGTLDHLVEASKAGFVFRCDDIDGLCLKNPTTYPGYHRQTADKETVICDLFFQSKKPLVNICYEGAIKDVGPKHYAGVDILHRYLHISSMNMNGFIGEFVEEFDDLAEFASGNSTFAVRNVDSYLYYMADVYSSSVVPGGCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFZ1</accession>
    <name>C5DFZ1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0D01078p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0D01078g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552771.1">
        <property type="nucleotide sequence ID" value="XM_002552725.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFZ1"/>
    <dbReference type="FunCoup" id="C5DFZ1">
        <property type="interactions" value="246"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFZ1"/>
    <dbReference type="GeneID" id="8294991"/>
    <dbReference type="KEGG" id="lth:KLTH0D01078g"/>
    <dbReference type="eggNOG" id="KOG1895">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021804_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFZ1"/>
    <dbReference type="OMA" id="YKNFVQF"/>
    <dbReference type="OrthoDB" id="331600at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021850">
        <property type="entry name" value="Symplekin/Pta1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032460">
        <property type="entry name" value="Symplekin/Pta1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15245:SF20">
        <property type="entry name" value="SYMPLEKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15245">
        <property type="entry name" value="SYMPLEKIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11935">
        <property type="entry name" value="SYMPK_PTA1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Symplekin/Pta1 N-terminal" evidence="5">
        <location>
            <begin position="87"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="455"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="485"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF11935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR22333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="719" mass="81443" checksum="F15839D15FBF6BAB" modified="2009-07-28" version="1">MSSEVLQLAQARELAMDNKPGEMLPKVLDTAMALHRSSRSPSVELGRLCAKLFLDVASHEAVVSSEKPFLASQHLGELWKLCSSSTDHSTLRDGILGLSRCYDWLFDLVAKTSNKELWETLCQFRSFVLSKWKTVYPLTPSEDSLQDHSRSLGVKLATVKFISTLVIVHTPGNSGISIASVPENHPVITGKSQLESGAKKLLGFLLSYLSEEPMMVASLFIGVLNCLAFVMKRRPQATYHILGGLLRFNVDMKYQQNAESVLQYRLAKRFVERCYKVFVQFGLKSQLIKNSGNMAQYHAKLSKISQTLHIIGEETKAKGIMNFDPKQVERKMPSRERAKYVTALKASNQNSSSRNNSTQPRMSPDMQLLTELQKYTMSKNSSAGFFNTSPIAFDSTYASVYSLMNSKHSEIDVSKLSPAVMAKLCTECLYQTDTNKMISGLSIVASRYTDLMNKASQATDNKKRPLEADTVQQPSKKREVVKQPEDEELSEDEDKQEFTLGAPAPMSEEEKKQHFIRIVGHIMAVKDSEEEPGASNEVPVNMLHKVRLLDWNNKTSWLTLLTRLASRGVSHNEEMSNTARQAIYDYFIEDFGNRVAVVIEWLSEEWYYESLNNEKGETPVYDQWSLKILDALIPFLEVSHRRLFIRLVSELPRLSQEHVDKIRSLCLDPLRGSLGFQSLKFMVMFRPPVKPHIAKLLTSMKEQDESVREQCEAVLAKFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DG01</accession>
    <name>C5DG01_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Holocytochrome c-type synthase</fullName>
            <ecNumber evidence="10">4.4.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0D01320g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Lyase that catalyzes the covalent linking of the heme group to the cytochrome C apoprotein to produce the mature functional cytochrome.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>holo-[cytochrome c] = apo-[cytochrome c] + heme b</text>
            <dbReference type="Rhea" id="RHEA:22648"/>
            <dbReference type="Rhea" id="RHEA-COMP:10725"/>
            <dbReference type="Rhea" id="RHEA-COMP:10726"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <dbReference type="ChEBI" id="CHEBI:83739"/>
            <dbReference type="EC" id="4.4.1.17"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the cytochrome c-type heme lyase family.</text>
    </comment>
    <dbReference type="EC" id="4.4.1.17" evidence="10"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552781.1">
        <property type="nucleotide sequence ID" value="XM_002552735.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DG01"/>
    <dbReference type="FunCoup" id="C5DG01">
        <property type="interactions" value="368"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DG01"/>
    <dbReference type="GeneID" id="8295001"/>
    <dbReference type="KEGG" id="lth:KLTH0D01320g"/>
    <dbReference type="eggNOG" id="KOG3996">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048602_0_1_1"/>
    <dbReference type="InParanoid" id="C5DG01"/>
    <dbReference type="OMA" id="KARFWLF"/>
    <dbReference type="OrthoDB" id="1158011at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004408">
        <property type="term" value="F:holocytochrome-c synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000511">
        <property type="entry name" value="Holocyt_c/c1_synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12743">
        <property type="entry name" value="CYTOCHROME C1 HEME LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12743:SF3">
        <property type="entry name" value="HOLOCYTOCHROME-C SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01265">
        <property type="entry name" value="Cyto_heme_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00821">
        <property type="entry name" value="CYTO_HEME_LYASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00822">
        <property type="entry name" value="CYTO_HEME_LYASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0349">Heme</keyword>
    <keyword evidence="6 10" id="KW-0408">Iron</keyword>
    <keyword evidence="9 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 10" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR22343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="274" mass="31156" checksum="D59D4B4E21627E89" modified="2009-07-28" version="1">MGWFWADSGKNSTSGNKITDKPSECPLGHSTSKVTSGADGCPVLHGSNAQNEINPFNNMPKNLSHEKHIGQKLELPKDRTTSSIPKGNPGEEEFWEYPSPQQMYNAMIRKGKIDPNTGEEIPEDAVESMVFVHNFLNEGCWQEILDWEKKYSEQTEMEPKLLQFMGKPSQLSPRARCFSILGTLFPSKFSAELPFDRHDWVVLRPDPTSSDTKNPGFRQVRYVIDFYGGPDDEDGLPTFNLDVRPALDNFTNAKDRFTRWTEPIIQEYFGSDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DG72</accession>
    <name>C5DG72_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0D02904g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the phosphatase 2A regulatory subunit B family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552852.1">
        <property type="nucleotide sequence ID" value="XM_002552806.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DG72"/>
    <dbReference type="FunCoup" id="C5DG72">
        <property type="interactions" value="740"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DG72"/>
    <dbReference type="GeneID" id="8295072"/>
    <dbReference type="KEGG" id="lth:KLTH0D02904g"/>
    <dbReference type="eggNOG" id="KOG2085">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012437_1_0_1"/>
    <dbReference type="InParanoid" id="C5DG72"/>
    <dbReference type="OMA" id="MYAHVVN"/>
    <dbReference type="OrthoDB" id="10264446at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005816">
        <property type="term" value="C:spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051754">
        <property type="term" value="P:meiotic sister chromatid cohesion, centromeric"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901991">
        <property type="term" value="P:negative regulation of mitotic cell cycle phase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098813">
        <property type="term" value="P:nuclear chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000016">
        <property type="entry name" value="Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002554">
        <property type="entry name" value="PP2A_B56"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10257">
        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE 2A PP2A REGULATORY SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10257:SF3">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 2A 56 KDA REGULATORY SUBUNIT GAMMA ISOFORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01603">
        <property type="entry name" value="B56"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028043">
        <property type="entry name" value="PP2A_B56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="132"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="22"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="32"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="84"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="105"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="138"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00064351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR028043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR22414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="655" mass="74079" checksum="2B7A4C1D3B775038" modified="2009-07-28" version="1">MMRGFKQKLIKKTTGSSSSGQKKKDKEEAKKTSSGTSSPGSASGTSKSAAEKKSASSSSSSKHTKSKTATNNGSEKKSNPPPSSAQNDSPNAAPTSKNKLEVMGNINQPSRSSQPTLIAAAALPPSPTTATVSLAVPGTQSSKEPTTASGIEIPRSSHSFERLPNASKLNPEADLDLIKTPQRHSSSRFEPSRYTQITKLPHFDDVSPEEQIPLFIAKVDQCNTIFDFGDPSFDIQGKEIKRVTLQELIEFVVTNRFAYTEEMYAHVVNMFKNNLFRPIPPPVNPIGDVFDPDEDEPVNELAWPHMQCIYEFFLRFVESPDFNHQIAKQYIDQDFILRLLELFDSEDIRERDCLKTTLHRIYGKFLSLRSFIRRSINNIFLQFVYETERFNGIAELLEILGSIINGFALPLKEEHKVFLVRVLMPLHKVRCLSLYHPQLAYCIVQFLEKEPLLTEEVVMGLLRYWPKINSTKEIMFLNEIEDIFEVIEPLEFIKVEVPLFVQVAKCISSSHFQVSEKVLSYWNNEYFLNLCIENAEVILPIIFPALYELTSQLDLESQSDENGHPTSDPYVLVEQAISSGSWNRAIHAMAFKALKIFLETNPVLYDNCNSLYLTSVKETQRRKVEREENWHRLESYVEKLKISGTAGGDESSPQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGH4</accession>
    <name>C5DGH4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0D05346p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0D05346g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552954.1">
        <property type="nucleotide sequence ID" value="XM_002552908.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGH4"/>
    <dbReference type="FunCoup" id="C5DGH4">
        <property type="interactions" value="1066"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGH4"/>
    <dbReference type="GeneID" id="8295182"/>
    <dbReference type="KEGG" id="lth:KLTH0D05346g"/>
    <dbReference type="eggNOG" id="KOG0310">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021102_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGH4"/>
    <dbReference type="OMA" id="ATYQVVH"/>
    <dbReference type="OrthoDB" id="431715at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045943">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000551">
        <property type="entry name" value="UTP15p Nucleolar protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001186">
        <property type="entry name" value="UTP15p Nucleolar protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018983">
        <property type="entry name" value="U3_snoRNA-assocProt_15_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19924:SF26">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 15 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19924">
        <property type="entry name" value="UTP15 U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 15 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09384">
        <property type="entry name" value="UTP15_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="3 6" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="136"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="164"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="U3 small nucleolar RNA-associated protein 15 C-terminal" evidence="7">
        <location>
            <begin position="360"/>
            <end position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF09384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR22516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="507" mass="56470" checksum="A4D7E7E0E35635C7" modified="2009-07-28" version="1">MSSSRPRATLVRAPVLPEQTTPEQRYWRSYGSTQLVKEHNSVTSISFNPTHPNDFAITSSTRVQLFSSRTRQVIKTFSRFKDVVYSASFRQDGKLLAMGDATGRVSIYDSYNPRTQLVSIAASSHPTHVAKFHPADSRTLVTASDDRVVRVWDITQAQEPQVELTGAADYVRSVCMVPQAPHLVVTGSYDGFVRLFDTRSNEAATTLVQGMPVEDVIAVSPTQLVSCGGPKFKVWDLTAQRQLLERVNFAKTVTCLDHVELMEDSPMSSTLVASSLDGHVKVFDPLDGYKVKFGWKFSSPVLSCAVSPGNAQGNKHLVAGLSSGLLAIRTKRRAEKSEKIDIEKVAKSSNFQRMMRGSEYAGDQEHIIHNDKTRPQRKLRAFERHMNQFKWAEALDAAFVSGMAKELTLTALHELRRRGKIRVALIGRDESSLEPLLNWCLRGIEDVRSATVVADWVAVVLELYGDLIAKSSVLEEMIVALKDKVKQEVHRAEEAQKIEGMLQLLVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGI1</accession>
    <name>C5DGI1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0D05500p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0D05500g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552961.1">
        <property type="nucleotide sequence ID" value="XM_002552915.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGI1"/>
    <dbReference type="FunCoup" id="C5DGI1">
        <property type="interactions" value="97"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGI1"/>
    <dbReference type="GeneID" id="8295189"/>
    <dbReference type="KEGG" id="lth:KLTH0D05500g"/>
    <dbReference type="eggNOG" id="ENOG502S0GH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018639_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGI1"/>
    <dbReference type="OMA" id="FNDSYLD"/>
    <dbReference type="OrthoDB" id="4085524at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="263"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="388"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="461"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="598"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="140"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="322"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="346"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="393"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="463"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="489"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="565"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="608"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="687"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="750"/>
            <end position="765"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="765" mass="84023" checksum="D9014051E6BA43F0" modified="2009-07-28" version="1">MFHSRRSRESRAIDSEALAAASAIGRALDSKGTKVNAEKLPRYNSLSRSNSMVRAASISSRNNSLRTRRDSRRSSSLQLKGPRVRDSIDESEFYDAEDTFHAFGGAQTQTKPVTVKRYVPGPSGLKLIEVPISEVQKPPSRNSSMNRRSASVHSGLSVRNNSLGRKTSLNSHASQKQKSSAPVTQKQKKEPMIRRSEPPKNNKPLVEQSLKEETDEQLQLDRQNKEVVNPIELTPPSKKAAPEVSILRLREKQVNIVTKTVDSGDLNETKQPPTVLISPPNEDISETQNHADLNDASPYPTEEEALKQATVSSEPAPRSGIRGKENESGKENTGKTVEDVIKEESNASSRNEANSPTQSASAEEGGGTNGNSMAKYIRSANQYLNKGHKKVLTSAQESNAGGLEQQASSQITGNGGGEMAPLNKVPSPMKPALKKTASDNSMRNPYKENFGSKAEEAYLSLATAENTRLNAQMSDEHSRRQPPVRKTRPSSMVTNATAVQKNIPAVTKPKHMSMQTPLRSEIRPPARSTMRPESIRQDKKIKPKSRTRPQQTINDKRAFFPPEPPQKRSSFEKIRPNETHMGFKKLSLRDEVSEEANYGPVVGENFHNDANSISTGRDSSSQIRSQASLPASNSGWKSRFHDSDSEDEYSPISTVSPAKPTAKPGVRNGGFSLRNKKDSGFAPPQPNFVQSSTAPNSKHNTPNKKISTLSLRSVSNKSDMSPAKGNNNLSQRFFSEQLSPSAEEGDTKKKSSFGKKLKKIFGRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGR3</accession>
    <name>C5DGR3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">KLTH0D07436p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0D07436g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LU7TM family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553043.1">
        <property type="nucleotide sequence ID" value="XM_002552997.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGR3"/>
    <dbReference type="FunCoup" id="C5DGR3">
        <property type="interactions" value="635"/>
    </dbReference>
    <dbReference type="GeneID" id="8295275"/>
    <dbReference type="KEGG" id="lth:KLTH0D07436g"/>
    <dbReference type="eggNOG" id="KOG2568">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024065_1_0_1"/>
    <dbReference type="InParanoid" id="C5DGR3"/>
    <dbReference type="OMA" id="TWGFYDF"/>
    <dbReference type="OrthoDB" id="19932at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053937">
        <property type="entry name" value="GOST_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009637">
        <property type="entry name" value="GPR107/GPR108-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053938">
        <property type="entry name" value="PTM1-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21229:SF1">
        <property type="entry name" value="GH17801P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21229">
        <property type="entry name" value="LUNG SEVEN TRANSMEMBRANE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06814">
        <property type="entry name" value="GOST_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21902">
        <property type="entry name" value="PTM1-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002947829" evidence="9">
        <location>
            <begin position="20"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="216"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="250"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="291"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="319"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="361"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="408"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="PTM1-like N-terminal" evidence="11">
        <location>
            <begin position="28"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="GOST seven transmembrane" evidence="10">
        <location>
            <begin position="181"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="460"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF06814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF21902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR22605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="488" mass="56004" checksum="9DDF3563A2C0DABE" modified="2009-07-28" version="1">MIAQGPFLVLAILANFAFGNRESLDGYRQVCSGMYSKEDWGGKVDPFISFNLKEISGDEGVSVAVLDFQDYEHLGIKSGERTFYICSDTAIEKGLCTEEHKNEYLFEEMIFDSETGENKTLAASIQTFYQTESGLHNPKYFVKSTGYYCVITHTENSNADFDAVVNFRNAFGQLPAAEINNLPLYGLLAIFYVVAMALYSFAFWKHKNEILPVQKYLLAFFAFLTVEQIFIWAYYDIKNEKGNTAGTKVYMVFVSILSAGKTSFSFFLLLVIALGYGVVYPKLNKKLMRRCQIFAGLNFSFSVAYMIQNYLTNPESVTLLPLITLVPAAITILGFYFWILKSMTETLRYLQEQRQVVKLKMYRRLLSTIYFSLFVLFAGIVVTSFVFIGMNSIEMVEQHWRTRFIVDFWPSLVYYFVFITVAFIWRPTDTSYMLACSQQLPTDPENVADFDLDDLQSLEEPSNHIDDDLNFSDDEDHQQGRNSPRQNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGU2</accession>
    <name>C5DGU2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0D08316p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0D08316g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553072.1">
        <property type="nucleotide sequence ID" value="XM_002553026.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGU2"/>
    <dbReference type="FunCoup" id="C5DGU2">
        <property type="interactions" value="174"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGU2"/>
    <dbReference type="GeneID" id="8295308"/>
    <dbReference type="KEGG" id="lth:KLTH0D08316g"/>
    <dbReference type="eggNOG" id="KOG0314">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019105_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGU2"/>
    <dbReference type="OMA" id="CGAKDHW"/>
    <dbReference type="OrthoDB" id="106784at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.60.10:FF:000005">
        <property type="entry name" value="E3 ubiquitin-protein ligase RBBP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014891">
        <property type="entry name" value="DWNN_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033489">
        <property type="entry name" value="RBBP6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025829">
        <property type="entry name" value="Zn_knuckle_CX2CX3GHX4C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15439:SF0">
        <property type="entry name" value="CELL DIVISION CYCLE AND APOPTOSIS REGULATOR PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15439">
        <property type="entry name" value="RETINOBLASTOMA-BINDING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08783">
        <property type="entry name" value="DWNN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13696">
        <property type="entry name" value="zf-CCHC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01180">
        <property type="entry name" value="DWNN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51282">
        <property type="entry name" value="DWNN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DWNN" evidence="8">
        <location>
            <begin position="5"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="7">
        <location>
            <begin position="175"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR22634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="396" mass="44139" checksum="AB9A9E2FB3B1FAD9" modified="2009-07-28" version="1">MSSTIFYKFRSQKGTSRILFDGTGITVFDLKREIIQENRLGDGTDFQLRLYNPDTGEELEDDSYVIPRSSNVEVRRSPAIKVSGLNGRTSLGNATRYITGKPRVMKATSHASPAVSAALNTKGMSEEDAIASMFANQENQWQQTQQEMSSATPIFHKTGAAAGQDEGPPPPGYMCYRCGGKDHWIKNCPTNSDPNFEGKRIRRTTGIPKKFLKSVEIDPSTMTPEEMAQRKIMVTDDGSFVVQVADQHSWEDYQRKQQSRVMNHADAIWAKDHFANLSEDLKCPITGGLLKAPVKTSKCCNKTFSRQALEDLLVEHDFVCPGCGAEETLLDTLVVDEEKEKQVEAFLSENSVKRKDADADGRDAKKPKLAMPVPPFGMPFPMFPMPFLPNMNPTKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DH71</accession>
    <name>C5DH71_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">KLTH0E01848p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0E01848g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ERD2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553569.1">
        <property type="nucleotide sequence ID" value="XM_002553523.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DH71"/>
    <dbReference type="FunCoup" id="C5DH71">
        <property type="interactions" value="400"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DH71"/>
    <dbReference type="GeneID" id="8291708"/>
    <dbReference type="KEGG" id="lth:KLTH0E01848g"/>
    <dbReference type="eggNOG" id="KOG3106">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057784_0_0_1"/>
    <dbReference type="InParanoid" id="C5DH71"/>
    <dbReference type="OMA" id="WKSRSCE"/>
    <dbReference type="OrthoDB" id="7694678at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046923">
        <property type="term" value="F:ER retention sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006621">
        <property type="term" value="P:protein retention in ER lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000133">
        <property type="entry name" value="ER_ret_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10585">
        <property type="entry name" value="ER LUMEN PROTEIN RETAINING RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00810">
        <property type="entry name" value="ER_lumen_recept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00660">
        <property type="entry name" value="ERLUMENR"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00951">
        <property type="entry name" value="ER_LUMEN_RECEPTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="64"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="101"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="157"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="187"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR23132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="220" mass="25772" checksum="56A3382288994667" modified="2009-07-28" version="1">MFNIFRISADLAHLASILILVHNIRKTRQIDGISLKTQVLYVLVFCTRYLDLLTFRWRSVYNTLMKFVFIGSSVYVVSLIQKCRVTNPVAYRDMLVRDTFHIKYLLAGSAVLALIFNYKFTFLEIFWSFSVWLESFAIMPQLFMISRSGKASTITSHYIFAMGLYRALYIPNWIWRYSIDGRPLDKLSFCTGLIQTALYSDFFYVYYKKVVKGSGFQLPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DH80</accession>
    <name>C5DH80_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0E02046p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0E02046g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553578.1">
        <property type="nucleotide sequence ID" value="XM_002553532.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DH80"/>
    <dbReference type="FunCoup" id="C5DH80">
        <property type="interactions" value="59"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DH80"/>
    <dbReference type="GeneID" id="8291717"/>
    <dbReference type="KEGG" id="lth:KLTH0E02046g"/>
    <dbReference type="eggNOG" id="KOG1861">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015513_4_0_1"/>
    <dbReference type="InParanoid" id="C5DH80"/>
    <dbReference type="OMA" id="WWSQTIP"/>
    <dbReference type="OrthoDB" id="199574at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045107">
        <property type="entry name" value="SAC3/GANP/THP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005062">
        <property type="entry name" value="SAC3/GANP/THP3_conserved"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12436">
        <property type="entry name" value="80 KDA MCM3-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12436:SF4">
        <property type="entry name" value="LEUKOCYTE RECEPTOR CLUSTER MEMBER 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03399">
        <property type="entry name" value="SAC3_GANP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="2">
        <location>
            <begin position="364"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="159"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR23141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="554" mass="63609" checksum="C8A29260DB7FCE3B" modified="2009-07-28" version="1">MDGAHHIPSKREVQISPSMKDQHGLSQVFDIDDVRERRMKKGGKKYNQVQPVALGQSRKIPSSENRKAMSEKEKTELLQRIIGGGSQQFPSGMREFILGCFQLAEQHKFDQSAHMLLVQQLKDLISKAYTEKKEYINDWWSQTIPCLTKSRTELVLACDKPSRKPSAPVSSPRQSSLPPPPPPPPPSQEAVRGTLRVPPPPPTVDMARRQNSNTVAPDRLASRSRGSTELERRRKRMERFSNDSTGSKKSKVTSDENFANLNAISTNFYKFDKNKPVVGRCQTLEKKYLRLTSEPNPDLVRPLNVLKKAYDSILEKHTKGEASYAYLCDQFKSMRQDLRVQIIENQFALKVYQTHARLALQNNDIGEFNQCQSRLGQLFELPNLPNSNLEEFVSYRILYYLMMNNQNSINELKLKYMTAENLAVYRHPIVRHALKMANSLLMDDYHSFFKLYAETSGPTRCLVDTFISRERLRALNTISKSYNQIPLPFLFKELQLQSFDEGVEFIEQLGLSQYVSYKNKGQEGELATLNTKTSRFSIIQQFEKSKRVDIKGQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DH81</accession>
    <name>C5DH81_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="8">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0E02068g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7 8">Belongs to the peptidase C12 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="8"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553579.1">
        <property type="nucleotide sequence ID" value="XM_002553533.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DH81"/>
    <dbReference type="FunCoup" id="C5DH81">
        <property type="interactions" value="582"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DH81"/>
    <dbReference type="MEROPS" id="C12.002"/>
    <dbReference type="GeneID" id="8291718"/>
    <dbReference type="KEGG" id="lth:KLTH0E02068g"/>
    <dbReference type="eggNOG" id="KOG1415">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054406_0_2_1"/>
    <dbReference type="InParanoid" id="C5DH81"/>
    <dbReference type="OMA" id="IDLHYVC"/>
    <dbReference type="OrthoDB" id="427186at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.532.10">
        <property type="entry name" value="Peptidase C12, ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001578">
        <property type="entry name" value="Peptidase_C12_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036959">
        <property type="entry name" value="Peptidase_C12_UCH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057254">
        <property type="entry name" value="UCH_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10589">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10589:SF17">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01088">
        <property type="entry name" value="Peptidase_C12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00707">
        <property type="entry name" value="UBCTHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00140">
        <property type="entry name" value="UCH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52048">
        <property type="entry name" value="UCH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7 8" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7 8" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="4 7 8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UCH catalytic" evidence="9">
        <location>
            <begin position="6"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="93"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="166"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="7">
        <location>
            <position position="87"/>
        </location>
    </feature>
    <feature type="site" description="Important for enzyme activity" evidence="7">
        <location>
            <position position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS52048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR23142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="233" mass="26289" checksum="53530D30F7461D1E" modified="2009-07-28" version="1">MSLPRSVVPLESNPEVFTRFAHELGLSPEYAVHDIYSLTEPDLMAFLPRPMKAIVLLFPINDVYESSKDTSTRNEGASQASPIWFKQTVKNACGLYALLHSLANNPDTLQENSKISHFLSSNPRPDNRFADEKTDEFIVSISELYNKNSSYGQTAVPQAEDEVNLHFITYVKSGDYIFEMDGRRNGANLLGTSSSQDLLEEPLVRERVEWYMNNADENMKLSFSLLGLAPSWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>C5DH89</accession>
    <name>C5DH89_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0E02244p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0E02244g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553587.1">
        <property type="nucleotide sequence ID" value="XM_002553541.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DH89"/>
    <dbReference type="FunCoup" id="C5DH89">
        <property type="interactions" value="237"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DH89"/>
    <dbReference type="GeneID" id="8291726"/>
    <dbReference type="KEGG" id="lth:KLTH0E02244g"/>
    <dbReference type="eggNOG" id="ENOG502S9HM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2133960_0_0_1"/>
    <dbReference type="InParanoid" id="C5DH89"/>
    <dbReference type="OMA" id="QHHHFAR"/>
    <dbReference type="OrthoDB" id="4038621at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="129" mass="14410" checksum="BAC2EF6823E48C52" modified="2009-07-28" version="1">MYRDSHFQSHHMLRSHHVLYLDWNNDVFTAPDQPVSNAPRAGSSALPINPFWDYFHDDDDWDRFHPLQVDCSGQLTAMPPLVPTPTALGSLFAPRRAELGAWRDTGDLTSADLADRDPDAQAQGQGPDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHA2</accession>
    <name>C5DHA2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">KLTH0E02618p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0E02618g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SEC23/SEC24 family. SEC24 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553600.1">
        <property type="nucleotide sequence ID" value="XM_002553554.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHA2"/>
    <dbReference type="FunCoup" id="C5DHA2">
        <property type="interactions" value="181"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHA2"/>
    <dbReference type="GeneID" id="8291743"/>
    <dbReference type="KEGG" id="lth:KLTH0E02618g"/>
    <dbReference type="eggNOG" id="KOG1984">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004589_1_0_1"/>
    <dbReference type="InParanoid" id="C5DHA2"/>
    <dbReference type="OMA" id="RLCKHGD"/>
    <dbReference type="OrthoDB" id="49016at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1670">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.730">
        <property type="entry name" value="Sec23/Sec24 helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012990">
        <property type="entry name" value="Beta-sandwich_Sec23_24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036180">
        <property type="entry name" value="Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006900">
        <property type="entry name" value="Sec23/24_helical_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036175">
        <property type="entry name" value="Sec23/24_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006896">
        <property type="entry name" value="Sec23/24_trunk_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050550">
        <property type="entry name" value="SEC23_SEC24_subfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006895">
        <property type="entry name" value="Znf_Sec23_Sec24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036174">
        <property type="entry name" value="Znf_Sec23_Sec24_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13803">
        <property type="entry name" value="SEC24-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13803:SF4">
        <property type="entry name" value="SECRETORY 24CD, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08033">
        <property type="entry name" value="Sec23_BS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04815">
        <property type="entry name" value="Sec23_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04811">
        <property type="entry name" value="Sec23_trunk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04810">
        <property type="entry name" value="zf-Sec23_Sec24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82754">
        <property type="entry name" value="C-terminal, gelsolin-like domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81811">
        <property type="entry name" value="Helical domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82919">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Zinc finger Sec23/Sec24-type" evidence="9">
        <location>
            <begin position="191"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 trunk" evidence="10">
        <location>
            <begin position="270"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 beta-sandwich" evidence="12">
        <location>
            <begin position="511"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 helical" evidence="11">
        <location>
            <begin position="606"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF08033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR23163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="902" mass="101085" checksum="099ED6C9628F650C" modified="2009-07-28" version="1">MQRLNQSLSSLSLDRDSPSPIGVTRKNKRPNRAFHSFATDSYGLPDPQASAQNNFQPLAASNLGNISGNQASNFRVPFPSPSQRVSSGGPAESITSHYVPKQRLEDQQRYLTRSFVTSQDSVPPCVSTQYYCIDQGISDPRKLCLTMYNVPRNEQLRSATKLPVGAIIQPFAASVPDTAIDIIDASQRNGPLRCRRCRSYSNPNYSFTFDSKAVCNFCQVSTQLSEEHYAPLNPNGLRADINERPDLTKGTVEFLVPDTYNTVPGKPNIPLHYVFLVDISTLSNENKSSLAMVEGIRTCIEHISAKQPNCKVAIMAFDKEIRFFNLRPELNQAQEYIISDLQDVFVPIFTGLFVRPEESMHVIQDTLCKITAYIEDGKFSHHFEACYGSALKAARLALDTVTGGAGGKILASLSSKPTHGIGNLRLRNEDALKKTLKCENEAYVKLGQELLKNNISLDLFCTGSAFMDMVSVAQPVKVTSGILKYYPGFQLDKDEFTFVNDLLHSVANTVGYAGQLKVRCSSGLSVYNYYSESVENSDREPVIPVLHQDTTVNVLFKYDGRLPPAKDVHFQCALLYTDLNGARKVRCLNASAAVSDNIHEIFKFINQDAVVGIVLHDMLTTLDDCNFVQKRKTIDDKIVDVLTQYRALVSKSSSSQLVLPDSLKTWAAYLLSFEKSELMKNNNKSANGNSRVYDMFKYLNFTPAQLVFKLYPQILPLHELLQESDYTFYDENELLLQAAPLENLCVRASRSELVDGGCYLIFDGTTAYLWFNENTNTYLLRDLLDVDTDSVKHSEIALIGNAIPTLDSGINLKARSVLRNWAQVSGRSYVPVSPLRPHVDVYYSHTMAALLVEDRSIEMIEAYDAYLVSLHKRINEKVKREDYTKVGNPKDHEHITQKFIQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHB5</accession>
    <name>C5DHB5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0E02948p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0E02948g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily. MetX family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553613.1">
        <property type="nucleotide sequence ID" value="XM_002553567.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHB5"/>
    <dbReference type="STRING" id="559295.C5DHB5"/>
    <dbReference type="GeneID" id="8291758"/>
    <dbReference type="KEGG" id="lth:KLTH0E02948g"/>
    <dbReference type="eggNOG" id="ENOG502QR3J">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028760_7_0_1"/>
    <dbReference type="InParanoid" id="C5DHB5"/>
    <dbReference type="OMA" id="HPILVMG"/>
    <dbReference type="OrthoDB" id="444135at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004414">
        <property type="term" value="F:homoserine O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009001">
        <property type="term" value="F:serine O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009092">
        <property type="term" value="P:homoserine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00296">
        <property type="entry name" value="MetX_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008220">
        <property type="entry name" value="HAT_MetX-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01392">
        <property type="entry name" value="homoserO_Ac_trn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001209">
        <property type="entry name" value="PRK00175.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32268">
        <property type="entry name" value="HOMOSERINE O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32268:SF16">
        <property type="entry name" value="SERINE O-SUCCINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000443">
        <property type="entry name" value="Homoser_Ac_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="109"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR23176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="489" mass="54858" checksum="2B8F0BF6FAF34FD7" modified="2009-07-28" version="1">MLLKTYPSLIRGLHRSLPRCSRSSNPAMQFPCLDKMEERSAQLKKNTISCSSTDLTSSPNNEPVYGKVRSGFQIFKSNSPLFLDYGGVLPNFQIAYETWGTLNSDRSNAVLVHTGLSASSHAKSSESNLQPGWWEKFIGPGNPVLDTDKLFVICTNILGGCYGSTGPSSPDPADGAPYATRFPMLSVQDIIRGQQELVKECLGIDKLYASVGSSMGGMQSLAYAQQFPEAVEKVVTVSACARSHPSSIAMRHAQRQVLMADPHWSRGFYYPTEAEPNKILPHVGMKLAREIATVTYRSGPEWESRFGTERLDDERTPVLCPDFLIETYLDHQGEKFSLEYDPNSFLYLSKTMDLFDLSQSHQERSQRRRKEAFEQHRHQQVGNLTARLSDEPYEPVKRRRTTTVEEAREDLRVGMESIRGKNVLVIGVKSDGLFPYWQQREIVDLLGGGPEVEHVELDESQSLYGHDTFLLDLEHVGGSIGKFLMKSFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHC0</accession>
    <name>C5DHC0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0E03058p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0E03058g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553618.1">
        <property type="nucleotide sequence ID" value="XM_002553572.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHC0"/>
    <dbReference type="FunCoup" id="C5DHC0">
        <property type="interactions" value="856"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHC0"/>
    <dbReference type="GeneID" id="8291763"/>
    <dbReference type="KEGG" id="lth:KLTH0E03058g"/>
    <dbReference type="eggNOG" id="KOG2020">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003712_0_0_1"/>
    <dbReference type="InParanoid" id="C5DHC0"/>
    <dbReference type="OMA" id="IAKRSWG"/>
    <dbReference type="OrthoDB" id="2215036at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042565">
        <property type="term" value="C:RNA nuclear export complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005049">
        <property type="term" value="F:nuclear export signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006405">
        <property type="term" value="P:RNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045478">
        <property type="entry name" value="Exportin-5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045065">
        <property type="entry name" value="XPO1/5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11223">
        <property type="entry name" value="EXPORTIN 1/5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11223:SF3">
        <property type="entry name" value="EXPORTIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19273">
        <property type="entry name" value="Exportin-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exportin-5 C-terminal" evidence="2">
        <location>
            <begin position="331"/>
            <end position="1169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1198"/>
            <end position="1220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR23181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1220" mass="141248" checksum="F2455A1C52CB512D" modified="2009-07-28" version="1">MDENGASQVVGALEAIYSPKSDNARRLEAQKFLDEVKMHEESPLWGYEIALNNPGNSILKHYGLGLLAFAIKRRWADYDQNRRIALRKWVVELNYRVQESDPRYIKEKLAFLWVEVAKCVWGEALKDDDPTDQQLEESWVGMDNDLSALWQLSEASRELTLIIFRTLFEDVFLVEDLTVLKRISVIQPLCVMVISPMDVFTARYRFTDKWTLFKSNGNGWFEHWVSELRAALTVGNSVYVVRLLETLKTCLNWPLSEIMIRNDVCGLLLECLLSNIPKAQSMALDSLHILLTRPYNDESHYQTVINRVFSSMDLLDKVYDNLQFDPNEIDEQKYPIVKKFVDMVSCLYTCVFKTDEDEATIQKYLRLVLRTTFNPSLIVSGLTLDLWCSCLRNDDFLPALEGSIIPELLQFSADALIYYEQIENHVSKKFADIDFQSKSEFQSFCSTYRKRIRDIIRLISCVQLDFAYDWLNARLNSYFSSPFGQQVLSSQFLDHKTEPYLSSLSQLMVVECFINGCIRWKIWFPDTSSYNTKLNEILVKIETLSDQLIALNLKEPLLLKKQIQNFALFLTMLKDNVLLKLLEKIITSATLDYPNVDLDEKNEHSDAVRDLRYACGIELNRMAILMPDSLGKIYDDLQNVVAGIMPKLSYHEKISFKSFLLTIVLKSSLGEKEERFTLIVDPELSAWSDKSTVVGLTDLPWFMERLGIVQISEYFQKRGISENVDLLSIPIDEEGKQLKTQLSKRWQTLFPVRATRMFVHYSMQSIKNDEEFEVLQALWKPRVIPILPYIMRLLYQLQSYHDPENWRDLPVIVQSFVKCSTIERFWEAGASNKSKDEFIDEHMKAMQTLRDFADSVGHIVRYTREYVLLVISAISSLGSVFYEIEELPQMLMDSIAIYKPATGEISPGVSTHGWKHIINVAIRPLLKNCPPRSAKKFMTTFLPKLFDTLDALLCKKWSVYMNDIDVNPSPRDDDEMTEEILEENLLRQLTTVVVRLLIDCVGQVGTNSQASKMKLNSHQIEMRKIIFGNSEVMASFLKLLNHLMSFRDSKCSFNSILVMKSCLVDTLIKNESVDQFFTTEIMPNLLLNVLTQNAFKDSLYEGLYVFTVIFLTLCKEYKSSIQYLCQLSNGFDVESLYESVRSVENYKSQRALMVEFIDWIKTVNGNNMEDQDDDDKRRQEKRQILLERANERLIKKNKEQKDILDDPNTEDGAFGSLFTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHQ2</accession>
    <name>C5DHQ2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Xaa-Pro aminopeptidase</fullName>
            <ecNumber evidence="5">3.4.11.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">KLTH0E06160g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of any N-terminal amino acid, including proline, that is linked to proline, even from a dipeptide or tripeptide.</text>
            <dbReference type="EC" id="3.4.11.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the peptidase M24B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.9" evidence="5"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553750.1">
        <property type="nucleotide sequence ID" value="XM_002553704.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHQ2"/>
    <dbReference type="FunCoup" id="C5DHQ2">
        <property type="interactions" value="399"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHQ2"/>
    <dbReference type="MEROPS" id="M24.A10"/>
    <dbReference type="GeneID" id="8291903"/>
    <dbReference type="KEGG" id="lth:KLTH0E06160g"/>
    <dbReference type="eggNOG" id="KOG2413">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011781_2_2_1"/>
    <dbReference type="InParanoid" id="C5DHQ2"/>
    <dbReference type="OMA" id="EPGMILS"/>
    <dbReference type="OrthoDB" id="9995434at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01085">
        <property type="entry name" value="APP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.350.10:FF:000015">
        <property type="entry name" value="Xaa-Pro aminopeptidase app-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.230.10:FF:000007">
        <property type="entry name" value="Xaa-Pro aminopeptidase P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000587">
        <property type="entry name" value="Creatinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033740">
        <property type="entry name" value="Pept_M24B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032416">
        <property type="entry name" value="Peptidase_M24_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001131">
        <property type="entry name" value="Peptidase_M24B_aminopep-P_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050422">
        <property type="entry name" value="X-Pro_aminopeptidase_P"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43763">
        <property type="entry name" value="XAA-PRO AMINOPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43763:SF6">
        <property type="entry name" value="XAA-PRO AMINOPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01321">
        <property type="entry name" value="Creatinase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16189">
        <property type="entry name" value="Creatinase_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16188">
        <property type="entry name" value="Peptidase_M24_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53092">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00491">
        <property type="entry name" value="PROLINE_PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0464">Manganese</keyword>
    <keyword evidence="9 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Creatinase N-terminal" evidence="14">
        <location>
            <begin position="99"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M24" evidence="13">
        <location>
            <begin position="435"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M24 C-terminal" evidence="15">
        <location>
            <begin position="662"/>
            <end position="724"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF16188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAR23313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="724" mass="81765" checksum="1CEF04BF7EB663DD" modified="2009-07-28" version="1">MSTRQNTLNMKNSLSSMNTFGGATPVAGSARPSRPCANCTCSPGLLSRQNRRSSLLLRQVSSSRKRPILRTSVATGGSGESIYSSDTICQVKNVNTSERLLHLRRKMSEKEICCYVVPSEDEHQSEYVSQADQRRSFISGFTGSAGVACITRDLLNFNNDKPEGKSILSTDGRYFNQATQELDFNWTLLRQGEDKMTWPEWCVYEAKEMSLALGGKEARIGIDPKLISYDQVIRFQKLIKDKTDGSKAKVSLVPVVENLIDAIWSRFEPLPVRDANDLLLLNSHFTGETFKEKRERLMNYLNEKTPGCSTFCIAALDEISWLLNLRGSDIEYNPVFYAYLLVHNEETILFTDDPYDDKIKGYFEDNNIAVKAYEDVWSFLCSTATKTKSSNDVMAIASGSSWEIVRSLGNAPYKQIQSPLELLKAVKNDVEISNARAAQVKDAVCLVQYFAWLEEQLISKEALIDEYKAACKLVEIRKTQKNFMGNSFETISSTGPNAAIIHYAPPAENSAMIDPCRIYLCDSGSQFLEGTTDITRTLHFTKPSDEEVRNYTLVLKGNLALERLVIPQGTSGYNIDVIARQFLWQHGLDYRHGTGHGVGSYLNVHEGPIGIGFRPHLGNFALEKGNIITNEPGFYKDGEYGIRIENDMLVQKAEGLKFGEHEFLKFENITLVPYCRKLIDRKLLTHEEKAQINSYYKRIWHTVVPFIQPQSISYKWLKRETAPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>C5DI81</accession>
    <name>C5DI81_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">tRNA (guanine-N(7)-)-methyltransferase</fullName>
            <ecNumber evidence="11">2.1.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Transfer RNA methyltransferase 8</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">tRNA (guanine(46)-N(7))-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">tRNA(m7G46)-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">TRM8</name>
        <name evidence="13" type="ordered locus">KLTH0E10428g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>guanosine(46) in tRNA + S-adenosyl-L-methionine = N(7)-methylguanosine(46) in tRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42708"/>
            <dbReference type="Rhea" id="RHEA-COMP:10188"/>
            <dbReference type="Rhea" id="RHEA-COMP:10189"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74480"/>
            <dbReference type="EC" id="2.1.1.33"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="10 11">tRNA modification; N(7)-methylguanine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Forms a complex with TRM82.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.33" evidence="11"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553929.1">
        <property type="nucleotide sequence ID" value="XM_002553883.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DI81"/>
    <dbReference type="FunCoup" id="C5DI81">
        <property type="interactions" value="572"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DI81"/>
    <dbReference type="GeneID" id="8292094"/>
    <dbReference type="KEGG" id="lth:KLTH0E10428g"/>
    <dbReference type="eggNOG" id="KOG3115">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050910_3_1_1"/>
    <dbReference type="InParanoid" id="C5DI81"/>
    <dbReference type="OMA" id="LPNYFAK"/>
    <dbReference type="OrthoDB" id="47276at2759"/>
    <dbReference type="UniPathway" id="UPA00989"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106143">
        <property type="term" value="C:tRNA (m7G46) methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008176">
        <property type="term" value="F:tRNA (guanine(46)-N7)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000060">
        <property type="entry name" value="tRNA (guanine-N(7)-)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03055">
        <property type="entry name" value="tRNA_methyltr_TrmB_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025763">
        <property type="entry name" value="Trm8_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003358">
        <property type="entry name" value="tRNA_(Gua-N-7)_MeTrfase_Trmb"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00091">
        <property type="entry name" value="tRNA (guanosine(46)-N7)-methyltransferase TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23417">
        <property type="entry name" value="3-DEOXY-D-MANNO-OCTULOSONIC-ACID TRANSFERASE/TRNA GUANINE-N 7 - -METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23417:SF16">
        <property type="entry name" value="TRNA (GUANINE-N(7)-)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02390">
        <property type="entry name" value="Methyltransf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51625">
        <property type="entry name" value="SAM_MT_TRMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 11" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="3 11" id="KW-0820">tRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="181"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="123"/>
            <end position="124"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="158"/>
            <end position="159"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="256"/>
            <end position="258"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00060552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR23492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="283" mass="32998" checksum="1669211E95F176D4" modified="2009-07-28" version="1">MKAGMSLDDPSSKRQAYRLKKEENRKELKHVKIDAKASSEKMDLPRKRFYRQRAHSNPFSDHQLEYPLSPDEMDWDRLYPHFVDEATGAMTKEVTIADIGCGFGGLLIDLSPAFADKLILGMEIRVQVTNYVEDRIIALRTNHAETNAYQNINVLRANSMKFLPNFFHKAQLEKMFFCFPDPHFKQRKHKARIITNTLLSEYAYVLKEGGVVYTITDVLDLHEWMVKHLEEHPLFERLSTEWEDQDECVEIMRNATEEGKKVERKKGAKYVACFTRLPTPEIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIA6</accession>
    <name>C5DIA6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">KLTH0E11000p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0E11000g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the XPF family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553954.1">
        <property type="nucleotide sequence ID" value="XM_002553908.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIA6"/>
    <dbReference type="FunCoup" id="C5DIA6">
        <property type="interactions" value="985"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIA6"/>
    <dbReference type="GeneID" id="8292120"/>
    <dbReference type="KEGG" id="lth:KLTH0E11000g"/>
    <dbReference type="eggNOG" id="KOG0442">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002265_2_0_1"/>
    <dbReference type="InParanoid" id="C5DIA6"/>
    <dbReference type="OMA" id="THILDIM"/>
    <dbReference type="OrthoDB" id="361020at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000110">
        <property type="term" value="C:nucleotide-excision repair factor 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000014">
        <property type="term" value="F:single-stranded DNA endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000736">
        <property type="term" value="P:double-strand break repair via single-strand annealing, removal of nonhomologous ends"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901255">
        <property type="term" value="P:nucleotide-excision repair involved in interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20078">
        <property type="entry name" value="XPF_nuclease_XPF_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10130:FF:000009">
        <property type="entry name" value="UV endonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006166">
        <property type="entry name" value="ERCC4_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006167">
        <property type="entry name" value="XPF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047520">
        <property type="entry name" value="XPF_nuclease"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00596">
        <property type="entry name" value="rad1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10150">
        <property type="entry name" value="DNA REPAIR ENDONUCLEASE XPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10150:SF0">
        <property type="entry name" value="DNA REPAIR ENDONUCLEASE XPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02732">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00891">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="RuvA domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ERCC4" evidence="10">
        <location>
            <begin position="772"/>
            <end position="852"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR23517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1019" mass="117005" checksum="DA0086B0B87A49F6" modified="2009-07-28" version="1">MSLFIRDESDEEDLVLELSRHEEDRSKQIDDASNGEMLYPLLPDDGDSHMRPNIEEIRPVDIQLSLPLPFQQMIVEDLLVSEDSLLILGKGLGIEPIVANLLHVLATPTRINGQDKRSLVLLLNASEEDNEKILEELLELAWSGTEEDGQRPFSVVTADLLTVDQRRRLYLHGGIVSVTSRILIVDLLSGIIHPNKITGLVILHVENLHNYSNESFIAEMYRSVNRWGFIKAVSDEPEAFMTEFSPLKRKLKDLRLKRTLLWPRFHVEVSSSLNVRNENKVIEVKVSLTNSMSQIQFGLFECLKKCIDELNRKNPTLSLESWNADNCLHPNFFRSLYSVLTPNWHRISFESKQLVKDIGTLKKLLHALVSYDAVDFYEIIQVILDANKPSVSRKYSESPWLMAEESQAVLSYAKKRVFSDGNYELEELPKWEQLTSILDDIAVEQAKLGSSLGPTLIMCSEDRTCRQLRKIIGYSNRKEGARRFMLNKLRAYMNRRDLLKKTAEDIQEESRKEGTSAELSVSRSFAKEEVASKRRRTRGAASVAAVTRLKRATSNGGEDIESVMSVDDIEQHISQIIEDTDEGLIGDQEIDNLAQNFDEDNELLLTEETNIDTSELEPMIKNENTWAYGQNNFEYIDRGDQIVIEKFYNRANDVLLQELLPSHIIMYEPDLAFIRRVEMFKALHKDWPLKVYFMYYGDSFEEQSHLIAIKKEKDAFTRLIREHSGLAQHFEADEDLSRYKNLAQRKLLLNQSLRNSRLAGGQKGYDAVTGDVVVVDMREFRAPLPGLLYRYGVKVIPCMLTIGDYILSPQICVERKSISDLIGSFKNGRLNDQCKKMSRYYEYPTLLIEFDDGDSFSLEPFSEKRYNSASSTSHPIGSKLMQDEIQMQLAQLIMKFPSLRILWSSSPLQTVNLILDLKLGREQPDPTTCVEIGVNRKSSGAKDKTNHSNEKFKELLSIPGLSNVDYYTIIRRVKRFTNLQKLSVDQITDLVNDKNLAERIWHYVQTENEEKDNDIMLEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIC8</accession>
    <name>C5DIC8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GID complex catalytic subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Glucose-induced degradation protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0E11506g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the RMD5/GID2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553976.1">
        <property type="nucleotide sequence ID" value="XM_002553930.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIC8"/>
    <dbReference type="FunCoup" id="C5DIC8">
        <property type="interactions" value="624"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIC8"/>
    <dbReference type="GeneID" id="8292143"/>
    <dbReference type="KEGG" id="lth:KLTH0E11506g"/>
    <dbReference type="eggNOG" id="KOG2817">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020227_2_0_1"/>
    <dbReference type="InParanoid" id="C5DIC8"/>
    <dbReference type="OMA" id="PYSLPCH"/>
    <dbReference type="OrthoDB" id="1933281at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034657">
        <property type="term" value="C:GID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16652">
        <property type="entry name" value="dRING_Rmd5p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000143">
        <property type="entry name" value="Regulator of gluconeogenesis Rmd5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024964">
        <property type="entry name" value="CTLH/CRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045098">
        <property type="entry name" value="Fyv10_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037683">
        <property type="entry name" value="Rmd5_dRing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044063">
        <property type="entry name" value="ZF_RING_GID"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12170:SF3">
        <property type="entry name" value="GH10162P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12170">
        <property type="entry name" value="MACROPHAGE ERYTHROBLAST ATTACHER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10607">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51867">
        <property type="entry name" value="ZF_RING_GID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-Gid-type" evidence="10">
        <location>
            <begin position="342"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="RING-Gid-type" evidence="9">
        <location>
            <begin position="342"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00075398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00080744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR23539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="402" mass="46345" checksum="DC4082020B76EDE0" modified="2009-07-28" version="1">MSDLLKNLNTEFGKLKEGASDGQSHLRRCLKDTHEFKLNIKKLKSYLTKQMLEPGAGQDPVDDKISRKRQLAVDKLNKLHRQWDSGVKKHGKNALHQHTKFQKSVLNKIYDFDLDQVYVNQLPADARQHIEKSIGVHISRYSMSEIPKTDPEAMVQYLKEVYGVDPQVSSSYVEMSQIVRQLRGGNLEPCMRWCTEGSSLQFELHLLNAMYFLQAGDKVSTYQYLLKHIPSFMEKTKKTHLRHQVAPLLAQLVVSSEAKVNVEDQRKKCMKLFTKEYCAQSRLPFDSPLFLVVLSGVISFQFFIKYRTLRAVSHVDWSTKNELPFNVKLPEFLTNFHPIFICPVLKEETTQENPPYALPCHHIISKFSLDKMSKNGTCNFKCPYCPVMAARSKTSRVNFVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-04-02" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJ06</accession>
    <name>C5DJ06_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0E16632p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0E16632g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554204.1">
        <property type="nucleotide sequence ID" value="XM_002554158.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJ06"/>
    <dbReference type="STRING" id="559295.C5DJ06"/>
    <dbReference type="GeneID" id="8292376"/>
    <dbReference type="KEGG" id="lth:KLTH0E16632g"/>
    <dbReference type="eggNOG" id="ENOG502SAAE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1714345_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJ06"/>
    <dbReference type="OMA" id="TYTEGPD"/>
    <dbReference type="OrthoDB" id="4069481at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015891">
        <property type="term" value="P:siderophore transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035323">
        <property type="entry name" value="Fit2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17357">
        <property type="entry name" value="FIT1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950499" evidence="2">
        <location>
            <begin position="21"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="107"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR23767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="158" mass="15565" checksum="3F35608FC7888C4F" modified="2009-07-28" version="1">MKFSSTVLAASVIGASAVSAATSVMTTKTITATNGSNVYTKVVTDTADPIISQSTTRTVTVKNSDATYTKVVTEGPDTTSLSSTTKTITATNQSGSYTKTVTAAVTSSTSDSSSSSSSSSSSASSASSSSSSTGAAEGIMSNMNLGGALFVALASCML</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJ34</accession>
    <name>C5DJ34_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 12">Mannose-6-phosphate isomerase</fullName>
            <ecNumber evidence="5 12">5.3.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">KLTH0F13134g</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-mannose 6-phosphate = D-fructose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:12356"/>
            <dbReference type="ChEBI" id="CHEBI:58735"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="EC" id="5.3.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11 12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11 12">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 14">Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; alpha-D-mannose 1-phosphate from D-fructose 6-phosphate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the mannose-6-phosphate isomerase type 1 family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.8" evidence="5 12"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554760.1">
        <property type="nucleotide sequence ID" value="XM_002554714.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJ34"/>
    <dbReference type="FunCoup" id="C5DJ34">
        <property type="interactions" value="892"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJ34"/>
    <dbReference type="GeneID" id="8292984"/>
    <dbReference type="KEGG" id="lth:KLTH0F13134g"/>
    <dbReference type="eggNOG" id="KOG2757">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026967_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJ34"/>
    <dbReference type="OMA" id="DIGLFCG"/>
    <dbReference type="OrthoDB" id="6605218at2759"/>
    <dbReference type="UniPathway" id="UPA00126">
        <property type="reaction ID" value="UER00423"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004476">
        <property type="term" value="F:mannose-6-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009298">
        <property type="term" value="P:GDP-mannose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd07011">
        <property type="entry name" value="cupin_PMI_type_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.441.10:FF:000001">
        <property type="entry name" value="Mannose-6-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.441.10">
        <property type="entry name" value="Phosphomannose Isomerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001250">
        <property type="entry name" value="Man6P_Isoase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016305">
        <property type="entry name" value="Mannose-6-P_Isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018050">
        <property type="entry name" value="Pmannose_isomerase-type1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046456">
        <property type="entry name" value="PMI_typeI_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046457">
        <property type="entry name" value="PMI_typeI_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046458">
        <property type="entry name" value="PMI_typeI_hel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00218">
        <property type="entry name" value="manA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10309">
        <property type="entry name" value="MANNOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10309:SF0">
        <property type="entry name" value="MANNOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01238">
        <property type="entry name" value="PMI_typeI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20511">
        <property type="entry name" value="PMI_typeI_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20512">
        <property type="entry name" value="PMI_typeI_hel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001480">
        <property type="entry name" value="Mannose-6-phosphate_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00714">
        <property type="entry name" value="MAN6PISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00965">
        <property type="entry name" value="PMI_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00966">
        <property type="entry name" value="PMI_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Phosphomannose isomerase type I catalytic" evidence="16">
        <location>
            <begin position="5"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphomannose isomerase type I helical insertion" evidence="17">
        <location>
            <begin position="169"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphomannose isomerase type I C-terminal" evidence="15">
        <location>
            <begin position="344"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="300"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001480-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001480-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF20511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF20512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAR24323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="429" mass="47685" checksum="81519C438E12B4DA" modified="2009-07-28" version="1">MSNRLFRLDAGFQQYDWGKIGSSSAVAQFASHSDPSVAIEEDKPYAELWMGTHHKVPSLNHDTKQNLRDLIAAEPAKMLGQGNVEKFGSANELPFLFKVLSIEKVLSIQAHPDKELAKKLHMNDPSNYPDDNHKPEMAIAVTDFEGFCGFKPLEEIADELKRIPELRNIVGEEVSNQFVENYRASAVAGSAEDAENRKLLQSVFSKVMHATQEEITANSRSLIKRASDSPSDFNKKELPDLLIRLNKQFPDDVGLFCGGLLLNHCSLKAGEAIFLRAKDPHAYISGDIIECMAASDNVVRAGFTPKFKDVTNLVDMLTYAYDSVEDQKMKLEEFPRSSGDGKTVLYNPPIEEFAVLETTFKSGPGVRRFEGLQGPSILITTKGNGFIKSGETKLKSESGYVFFIAPETEVQVIAEDTDFTTYRAFVEPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJ91</accession>
    <name>C5DJ91_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0F14498g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554817.1">
        <property type="nucleotide sequence ID" value="XM_002554771.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJ91"/>
    <dbReference type="FunCoup" id="C5DJ91">
        <property type="interactions" value="397"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJ91"/>
    <dbReference type="GeneID" id="8293045"/>
    <dbReference type="KEGG" id="lth:KLTH0F14498g"/>
    <dbReference type="eggNOG" id="KOG0239">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001485_12_4_1"/>
    <dbReference type="InParanoid" id="C5DJ91"/>
    <dbReference type="OMA" id="ETARDKW"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01366">
        <property type="entry name" value="KISc_C_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47972">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KLP-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47972:SF28">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KLP-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="3 4" id="KW-0505">Motor protein</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="7">
        <location>
            <begin position="363"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="190"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="10"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="447"/>
            <end position="454"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR24380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="702" mass="80392" checksum="939861A6AB51F48B" modified="2009-07-28" version="1">MESESPRTPPRSTASRLGTSSKIPSPNGPSSPKFKKRSASPRPPSVSASEHQSMVAHTKKQNGGSEGSSHMTAFYRENIKQLNQLQDIMFRKKSKLDSVKDELFEGKEQFKSLMLKLETLKEEKHVKLQQFKLKHNDLKKLGDEHSTREKFMIKGYDLEIQQLRAKSTAELNAMEGDYRRQLENLRFAKVRKLENSLDELRRDISKARGSIANNKENFESQLNECEVRHNYRKEEWLRTHQKELNALIEESSTLNRESEKLQAVLDNELKAKLLKKNEEIEVLNSKLLGFQEQLEQSRLGNRQLEDEISKYTSETIQSLAKRDELGHYVSSSTYELQQIGEILLKEETMRRKLHNELQELRGNIRVFCRLRPALENENYSSSSIEIEKFSDETGMQSITIKRDSKQHKFTFDRCFSAQETNGDVFSEIGQLIQSSLDGYNVCIFAYGQTGSGKTFTMLNPNDGIIPSTLNHIFLWVEKLKELGWVYEITSQFVEIYNENIKDLFKENDADVDDTGESLKFEIRHDNESRTTHITNITVCKLTSREMVNRMLRRALKMRSTAATVANSRSSRSHSVFIIKLDGYNTISGEKSTGTLNLVDLAGSERIHSLQPQAERLRETQNINKSLSCLGDVIHALGSADASKRHIPFRNSKLTYLLQYSLMGDSKTLMFVNVSPCANSMLETLNSLRFAAKVNSTKMAKRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJ92</accession>
    <name>C5DJ92_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0F14520p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0F14520g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RRP15 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554818.1">
        <property type="nucleotide sequence ID" value="XM_002554772.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJ92"/>
    <dbReference type="FunCoup" id="C5DJ92">
        <property type="interactions" value="406"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJ92"/>
    <dbReference type="GeneID" id="8293046"/>
    <dbReference type="KEGG" id="lth:KLTH0F14520g"/>
    <dbReference type="eggNOG" id="KOG2974">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058264_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJ92"/>
    <dbReference type="OMA" id="FVKQRFY"/>
    <dbReference type="OrthoDB" id="20949at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000460">
        <property type="term" value="P:maturation of 5.8S rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000470">
        <property type="term" value="P:maturation of LSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012459">
        <property type="entry name" value="Rrp15"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13245">
        <property type="entry name" value="RRP15-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13245:SF14">
        <property type="entry name" value="RRP15-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07890">
        <property type="entry name" value="Rrp15p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="208"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="59"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR24381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="249" mass="27449" checksum="59B6D378CDB8FD01" modified="2009-07-28" version="1">MVSNKSNMNSENSKGHQIRSVKGSENAKTAESKENSAAVVNRSDNEDEEPSSVDGQEAPSEEEIPDGSSSEESEEDESEEEDDDLPRKKKAKLGKHDDGSSGFSTAVNAILGSHLKAHDRKDPIMARKRGVVKKLESDKLEQKAKRALLAEKKKLLNKTRKKDIIPTVSAESESGAEIRDVLEKERRLRKIAQKGVVKLFNAILSTQVKTEKEGAEKLSEVKNRAEKKELLAELSKEKFLDLVKAAGDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJC8</accession>
    <name>C5DJC8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">KLTH0F15400p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0F15400g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554854.1">
        <property type="nucleotide sequence ID" value="XM_002554808.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJC8"/>
    <dbReference type="FunCoup" id="C5DJC8">
        <property type="interactions" value="108"/>
    </dbReference>
    <dbReference type="GeneID" id="8293085"/>
    <dbReference type="KEGG" id="lth:KLTH0F15400g"/>
    <dbReference type="eggNOG" id="KOG0758">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015166_16_3_1"/>
    <dbReference type="InParanoid" id="C5DJC8"/>
    <dbReference type="OMA" id="PIDCFRQ"/>
    <dbReference type="OrthoDB" id="2139348at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000064">
        <property type="term" value="F:L-ornithine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990575">
        <property type="term" value="P:mitochondrial L-ornithine transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000099">
        <property type="entry name" value="Mitochondrial carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624:SF31">
        <property type="entry name" value="MITOCHONDRIAL ORNITHINE TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="4"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="98"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="197"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR24417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="278" mass="30220" checksum="09E6791EC9A04F92" modified="2009-07-28" version="1">MDIQEALKDILYGSIAGAAGKVIEYPLDTIKVRLQTQPAHLFPTSWSCIKYTYANEGFLKGFYQGVSSPLVGAALENAVLFVTFNRAQNFLKQYESLSPLSLTVWSGAFAGACTSYVLTPVELVKCTLQVSNLKNSKTSHSKVWPTVKHIVSQNGISGLWRGQSSTFIRECAGGAVWFTTYESVKQYLANKRNDTENQTWELLTAGASAGVAFNASVFPADTIKSTAQTEHLGIVNATKRILARNGPAGLYRGLGITLIRAAPANAVVFYTYETLSNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJH3</accession>
    <name>C5DJH3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chromatin modification-related protein EAF1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Chromatin modification-related protein eaf1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13 15">ESA1-associated factor 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Vacuolar import and degradation protein 21</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ordered locus">KLTH0F16434g</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of selected genes principally by acetylation of nucleosomal histone H4 and H2A. The NuA4 complex is also involved in DNA repair.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EAF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554899.1">
        <property type="nucleotide sequence ID" value="XM_002554853.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJH3"/>
    <dbReference type="FunCoup" id="C5DJH3">
        <property type="interactions" value="304"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJH3"/>
    <dbReference type="GeneID" id="8293132"/>
    <dbReference type="KEGG" id="lth:KLTH0F16434g"/>
    <dbReference type="eggNOG" id="ENOG502QSEY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004795_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJH3"/>
    <dbReference type="OMA" id="KPLDCKN"/>
    <dbReference type="OrthoDB" id="5364245at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000484">
        <property type="entry name" value="Chromatin modification-related protein EAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014012">
        <property type="entry name" value="HSA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46459:SF1">
        <property type="entry name" value="E1A-BINDING PROTEIN P400"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46459">
        <property type="entry name" value="E1A-BINDING PROTEIN P400-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07529">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00573">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51204">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="5" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HSA" evidence="18">
        <location>
            <begin position="337"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="17">
        <location>
            <begin position="592"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="66"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="402"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="454"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="712"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="763"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="867"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="97"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="208"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="773"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="800"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="868"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="914"/>
            <end position="928"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00072841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00082479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAR24462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="928" mass="106005" checksum="BE4A27C8A1655A3E" modified="2009-07-28" version="1">MVKLDRTAAAEEVSSLESMIAERNKTLAELYCISRLHDFLSISDEQTLQSQIDVFLEANDIRKGHHFDPSTLPKFTQIEPSPDKRVSKSKSGTPVDSARKEREHIKHDRDERNFDRDKDSSKESKVRISQDIRMNGGVAQPSPSKADGIELELEKFPNGAVRSQFNSNKRPREDENVQPNGPQSIIKEEHASMAPGTPHPSHTAPKRQRLDPSSAEHKEPSMNLTKKRSSSLLRELQAKPRKTEELFHQDNINAKESVYLIMNDKVPSKIPHAVPLSELKYNAQTLPLIKLIPTAHKVLTTDIMNTALNECRITVVSSRIEELRRLGLWSLRQPKKFIDPFENKKKQTHWGKLLEEAEWVSCDFDEFKKYKIAVCLTIAQSVMDFWNYGKACCVKVNPKKPIASPETTADPAESDGNGHDVSLPDSEGEIDHPLTTALEQYPSIDTKVLLQESVQPEDTHPIPSVVEDSSQLPSSKQHASLPFKLHVSFDDFSLVERKILSDLPLNGGLEHESLDDKPQDVAPFDPISKATVMLDDDQFVKLVERQLIDEEPSLVPFSKRRGMFYGNRRSHYLRPPIAPSLRYLRYRTPTIWLPEDDQELVRNINTYAYNWELISAHISSRPTRSYCSNIERRTPWQCFERFVQLNERFQFIDMKGPRAHSAQMWLIEAHKLQQQQKRRISPLGVGEDSIQRGHRRLRWASMFEAMRKCFKKRENAPKPNPTQPRKPLDCKNTSVPTPAEMSQLKAQRDDALRRDIQMRRIAKQKLQAAAMGQSLSSNSASKAIPRNSPSSGQRRAPDTARSSIRGSSPENSVRITTQTPQQTHIKQLSESEFVESYARKILLQKPDFSPELALKAAKSQFKLLAVKQQQQQQQQQQQQQQLYNQQTPHRSDGLSRTPYATLKTEPSNEKILSPTPQDILQKMQQSKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJJ5</accession>
    <name>C5DJJ5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0F16918p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0F16918g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554921.1">
        <property type="nucleotide sequence ID" value="XM_002554875.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJJ5"/>
    <dbReference type="FunCoup" id="C5DJJ5">
        <property type="interactions" value="90"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJJ5"/>
    <dbReference type="GeneID" id="8293154"/>
    <dbReference type="KEGG" id="lth:KLTH0F16918g"/>
    <dbReference type="eggNOG" id="KOG0504">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052404_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJJ5"/>
    <dbReference type="OMA" id="KKWQPLP"/>
    <dbReference type="OrthoDB" id="9942608at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031624">
        <property type="term" value="F:ubiquitin conjugating enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14372">
        <property type="entry name" value="CUE_Cue5p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000064">
        <property type="entry name" value="Similar to CUE domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003892">
        <property type="entry name" value="CUE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041807">
        <property type="entry name" value="Cue5/Don1_CUE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16461">
        <property type="entry name" value="TOLL-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16461:SF5">
        <property type="entry name" value="TOLL-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02845">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00546">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51140">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CUE" evidence="2">
        <location>
            <begin position="108"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="199"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="299"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="366"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="17"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="302"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="316"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="370"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="425"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="442"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR24484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="455" mass="50280" checksum="DA7A81B6F9466B29" modified="2009-07-28" version="1">MAESENKETVDEVSLSEETQATGAQAAPADATEQVAAAISGVGPEKPEGASEKPETELEKPSGASEKPQDATEEPEGSGKAPQANSEHDESEERPALPKRRDPTGDVIKNPILLQLTEAFPGIELKYINAVLIASQGVLDPAFNALLYLSDPSFEAEAAIPNIEPKAAAAAPAQPPVSKTQLEQDELLARQLDAQYNKSRARNVARSMEDERHARERRIRKRQQEYERRMAQSGRPLSPEEQQYYAELEELEEGENDFLSQLVDKDLPEFREKVGRQVQETGKRVNEWLSGFRKNWAQDPLQQSQYSQSPQQTAQRDFSTKSSSGSDYDTHGRPSHRTARFNSFGAKVGDDSVDSASRLASHGISLYNKDDDEINNDDDDDGDVAPQLPSRNKQKEVKPETTYIDTPENGTRKKWQPLPPEPINGTPSKVNATATKGRGNADADEEFLINTDDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJK4</accession>
    <name>C5DJK4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0F17160p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0F17160g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554930.1">
        <property type="nucleotide sequence ID" value="XM_002554884.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJK4"/>
    <dbReference type="FunCoup" id="C5DJK4">
        <property type="interactions" value="2080"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJK4"/>
    <dbReference type="GeneID" id="8293164"/>
    <dbReference type="KEGG" id="lth:KLTH0F17160g"/>
    <dbReference type="eggNOG" id="ENOG502QQ2N">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007860_1_0_1"/>
    <dbReference type="InParanoid" id="C5DJK4"/>
    <dbReference type="OMA" id="RSRQTCF"/>
    <dbReference type="OrthoDB" id="2427554at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004366">
        <property type="term" value="F:glycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016287">
        <property type="term" value="F:glycerone-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052744">
        <property type="entry name" value="GPAT/DAPAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31605">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE O-ACYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31605:SF2">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE O-ACYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="437"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="488"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="531"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="4">
        <location>
            <begin position="61"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="618"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="661"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="570"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="618"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="628"/>
            <end position="642"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR24493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="696" mass="77878" checksum="C542FEBD18125A06" modified="2009-07-28" version="1">MASHVPKSETFKNPYNGESYGLATWVYDLVVFVFNVCFTIFFREIKIRGTHNMPCKGTPTILVCAPHANQFVDGALVMSQVRNLRGSRSRQTCLVTAESSYKKRFISIFSRSTGGIPVPRAQDNLKPVDPALEIYVPDWAEPTVLKCRVVGRNESPRLSSRFTPKSLVGMPSFLGNAQIAEIPDDETLVLTKPFKDNSQVRDLLTKGTNFKYAPKIDNTSVFQNVFNHLHSNGCVGIFPEGGSHDRPSLLPIKAGVAIMALGAAAADPSSKIHVVPCGLSYFHRNKFRSRAVLEFGQPIIVDGEMGKRYEADPRGQTSELLDKITQALYAVTVNAPDYETLMTIQAARRLYQPAIAKTPDGNGHLPLPLVVEMNRRLLIGYSKYKDDPRIRHLKAMVLQYNSKLYALGLRDHQVPKLTSKGGEWISLLTICTRVIRICFYLLLSLPGTILFMPVFVTCHYYAQKKAEDGLKKSLVKIKGTDLLATWKLLVALVLAPTLYVTYSLMLCYLVKHYPQLFESLWVIGTRTNGYTSVNFVFFYALLVSATYASFKTGETGMDLFKSLPPLAVSLVYTKRQLEDLKETRKQLSLEVTQVCNDLGPTVFPDFGRFITTQKAAERGEEDKDYVPRSRSSSVHSMESQMSNALSKVNSNVSLSDIPILAEGSNEQQDTIEVEKTEKPSLIGNLVRQRRQQEKQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJN6</accession>
    <name>C5DJN6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">KLTH0F17886p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0F17886g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the syntaxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554962.1">
        <property type="nucleotide sequence ID" value="XM_002554916.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJN6"/>
    <dbReference type="FunCoup" id="C5DJN6">
        <property type="interactions" value="311"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJN6"/>
    <dbReference type="GeneID" id="8293197"/>
    <dbReference type="KEGG" id="lth:KLTH0F17886g"/>
    <dbReference type="eggNOG" id="KOG3894">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069210_1_0_1"/>
    <dbReference type="InParanoid" id="C5DJN6"/>
    <dbReference type="OMA" id="YRIRTHI"/>
    <dbReference type="OrthoDB" id="342981at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019529">
        <property type="entry name" value="Syntaxin-18_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15959">
        <property type="entry name" value="SYNTAXIN-18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15959:SF0">
        <property type="entry name" value="SYNTAXIN-18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10496">
        <property type="entry name" value="Syntaxin-18_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="308"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="11">
        <location>
            <begin position="236"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="203"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR24525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="327" mass="38323" checksum="F99ED4267D1F63D7" modified="2009-07-28" version="1">MSDLTPLFKKYVTVFSEDEKRSIDRRAQDNRKPSKKYMVNDTFVKECSELLRHILELQKVVFSLKPQYESDSELSEREKDDFDTEVRLLIQQYFGKLKFLGRYEKKRQQIVKDQFLTNSDSELFSLFKSRDEQFELFHLTNNKHRDGVLQSLNMLLSSIFSSISRMQQQRLSRKKELESIDFNAQLYAPIHNMVGSVSQSPPIETTQQEVEQYKQTVSQLSQQQLQILETEHEELLNLKTQELESAENLGRTMVQISSLQNEIATHLQSQTQNIFTLLDNHDDVELDIQKGNRQLKKAQRRSGKSAKLIVYLSVIFGVLILFLDFIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DK57</accession>
    <name>C5DK57_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ribosomal RNA small subunit methyltransferase NEP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0F01958g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase NEP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554295.1">
        <property type="nucleotide sequence ID" value="XM_002554249.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DK57"/>
    <dbReference type="FunCoup" id="C5DK57">
        <property type="interactions" value="1035"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DK57"/>
    <dbReference type="GeneID" id="8292487"/>
    <dbReference type="KEGG" id="lth:KLTH0F01958g"/>
    <dbReference type="eggNOG" id="KOG3073">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055846_1_1_1"/>
    <dbReference type="InParanoid" id="C5DK57"/>
    <dbReference type="OMA" id="VHNTFEL"/>
    <dbReference type="OrthoDB" id="269804at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070037">
        <property type="term" value="F:rRNA (pseudouridine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18088">
        <property type="entry name" value="Nep1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1280.10:FF:000003">
        <property type="entry name" value="Ribosomal RNA small subunit methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005304">
        <property type="entry name" value="Rbsml_bgen_MeTrfase_EMG1/NEP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12636">
        <property type="entry name" value="NEP1/MRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12636:SF5">
        <property type="entry name" value="RIBOSOMAL RNA SMALL SUBUNIT METHYLTRANSFERASE NEP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03587">
        <property type="entry name" value="EMG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="8" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00071403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR23858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="252" mass="27860" checksum="A60EDF13E59B3484" modified="2009-07-28" version="1">MVEDSKVRDALKTGEKKSLPASLVPQAPMVLTSRDKVTQRMIVVLSMASLETHKISSSGPGGDKYALLNCDDHQGLLKKMGRDISEARPDITHQCLLTLLDSPINKAGKLQVYIQTSRGVLVEVNPTVRIPRTFKRFSGLMVQLLHKLSIRSVNSEEKLLKVIKNPITDHLPTKCRKVTLSFDAPVIRVQDFIEKLDKDESICVFVGAMARGKDNFADEFVDEKIGLSNYPLSASVACSKFCHGAEDAWGIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DK83</accession>
    <name>C5DK83_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ubiquitin fusion degradation protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0F02530g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UFD1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554321.1">
        <property type="nucleotide sequence ID" value="XM_002554275.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DK83"/>
    <dbReference type="FunCoup" id="C5DK83">
        <property type="interactions" value="1145"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DK83"/>
    <dbReference type="GeneID" id="8292513"/>
    <dbReference type="KEGG" id="lth:KLTH0F02530g"/>
    <dbReference type="eggNOG" id="KOG1816">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037790_1_0_1"/>
    <dbReference type="InParanoid" id="C5DK83"/>
    <dbReference type="OMA" id="VCMIETD"/>
    <dbReference type="OrthoDB" id="422728at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034098">
        <property type="term" value="C:VCP-NPL4-UFD1 AAA ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032182">
        <property type="term" value="F:ubiquitin-like protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.50:FF:000001">
        <property type="entry name" value="Ubiquitin fusion degradation protein 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.330.10:FF:000002">
        <property type="entry name" value="ubiquitin fusion degradation protein 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.50">
        <property type="entry name" value="Ubiquitin fusion degradation protein UFD1, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004854">
        <property type="entry name" value="Ufd1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042299">
        <property type="entry name" value="Ufd1-like_Nn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055417">
        <property type="entry name" value="UFD1_N1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055418">
        <property type="entry name" value="UFD1_N2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12555">
        <property type="entry name" value="UBIQUITIN FUSION DEGRADATON PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12555:SF13">
        <property type="entry name" value="UBIQUITIN RECOGNITION FACTOR IN ER-ASSOCIATED DEGRADATION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03152">
        <property type="entry name" value="UFD1_N1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24842">
        <property type="entry name" value="UFD1_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin fusion degradation protein UFD1 N-terminal subdomain 1" evidence="5">
        <location>
            <begin position="22"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin fusion degradation protein UFD1 N-terminal subdomain 2" evidence="6">
        <location>
            <begin position="123"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="207"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="248"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="301"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="248"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="262"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="312"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00074895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF24842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR23884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="351" mass="39150" checksum="CDE4391C5EC0414A" modified="2009-07-28" version="1">MFSGFGGFGGFNAGFVNIPQKFEDFFRCYPISMMNDRIRKDDANFGGKIFLPPSALNKLTMLNVRYPMLFELTVPDTKKVTHGGVLEFIAEEGRVYLPQWMMETLGVNPGSLLQIASTDVPLGQFVKIEPQSVDFLDISDPKAVLENVLRKFSTLTVDDIIEINYNSKLYRIKVLEVKPESSSNSICVIETDLVTDFAPPVGYVEPDYRTEHRKSQKPKLDPSAQGLGPMSQRIDYATSVKSTSTTRAFVGTGQSLSGRTANKSEERELERPMISLDNEPAPLRLPEGQLFFGFPVVLPKKASDSQDENTEESQFQGAGQSLRQSNKRRGKKDHSSPRPKAPKSPEVIEID</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DK85</accession>
    <name>C5DK85_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0F02574p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0F02574g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554323.1">
        <property type="nucleotide sequence ID" value="XM_002554277.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DK85"/>
    <dbReference type="STRING" id="559295.C5DK85"/>
    <dbReference type="GeneID" id="8292515"/>
    <dbReference type="KEGG" id="lth:KLTH0F02574g"/>
    <dbReference type="eggNOG" id="KOG1470">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014001_1_1_1"/>
    <dbReference type="InParanoid" id="C5DK85"/>
    <dbReference type="OMA" id="HFVYCME"/>
    <dbReference type="OrthoDB" id="75724at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008526">
        <property type="term" value="F:phosphatidylinositol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052578">
        <property type="entry name" value="PI_Transfer_CRAL-TRIO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45824">
        <property type="entry name" value="GH16843P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45824:SF5">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL TRANSFER PROTEIN PDR17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03765">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="1">
        <location>
            <begin position="143"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="374" mass="43021" checksum="0223BE4725B30982" modified="2009-07-28" version="1">MGLFSKRNVEVRKEYNKSDIIKCNKAIRPRSQRSGKSKRVTGLSKEELLVFGNVLKHFQQEGCTFPVQSPRVKDSEHCQKLPEQPLSSWEKCWLTADRILRFLRSAKWDQEEAIARLTNTVIWRREFGIIDNEGRFHSSLVEAASSENESGGMLLLGYDRSQRPILIVRPGRQNTTTSFAQVQHLIFMVESALVLMPPGVESMTVLIDFQTPAGIPFTRMPPISVSRQVLHLLQKHYPECLGKAILINIPWYGWNFLKLFHPLIDPTTRSKVRYEDAFDDHVEETQLENSYGGRLNFTYEHELYWPDLIQLLSERSRVVYKKFLELGGQIGQSEFDLKDVSDVVEQPTCDGDQDFSLMSHDHIGDGFPGEKHKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKB7</accession>
    <name>C5DKB7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0F03300p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0F03300g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554355.1">
        <property type="nucleotide sequence ID" value="XM_002554309.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKB7"/>
    <dbReference type="STRING" id="559295.C5DKB7"/>
    <dbReference type="GeneID" id="8292547"/>
    <dbReference type="KEGG" id="lth:KLTH0F03300g"/>
    <dbReference type="eggNOG" id="ENOG502RZM9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_530034_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKB7"/>
    <dbReference type="OrthoDB" id="3163292at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="1">
        <location>
            <begin position="13"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="518" mass="60210" checksum="4EC173FE6ACC5DE0" modified="2009-07-28" version="1">MDDKGNSRKALRSCVRCRKNKIKCDLQEKRPGSCTPCSRRGIECYVDYVVPHQRSQELINLFQNVEDVKRSMGKLTSSYEKIAREHVPFFCMDRQGFSPSVGSKVLKLAEGEFIIIALSGEGLRVNNYLINSEDIEVALKDFQDVVGTLLQLYERPDCGRSCVNETVIDESVGKYTVENLFHSEQLPFLLCVVNFYFEIPGLKYNELFDTILEDYCFMAIEDEKSGKNFDRRTLSKLIIGHSSSDFNFLFNGEMFIKKFTLYLFYHIVIYGVRYYMDCFMEKYIRTLENVRKKVNIEKNWEVKWVNFYIKVFGLMDNMKEVLEIGEENDPFLKVLEYDSLIVNSDISIDTRFPDVLKQCHQDVKPLLRRGHMRIPFVKEFMAQFITMNYFVANLKFKKCLNEMESMKYTGFNTKVISELGSEAKCLCCDVNKGENENKLYAERGSAEDVVVEDPEECSANGNMRAGVRKVRYEEYDVYDIVATCLTKDGYKDILRRSCCGLVRDMCEKVIIEGMIMKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKD5</accession>
    <name>C5DKD5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0F03762p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0F03762g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="CAR23936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_002554327.1"/>
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    <dbReference type="STRING" id="559295.C5DKD5"/>
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    <dbReference type="KEGG" id="lth:KLTH0F03762g"/>
    <dbReference type="eggNOG" id="KOG1878">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034967">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Homeodomain-like_sf"/>
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        <property type="entry name" value="N-CoR_corepressor"/>
    </dbReference>
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        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
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        <property type="entry name" value="NUCLEAR RECEPTOR CO-REPRESSOR RELATED NCOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13992:SF39">
        <property type="entry name" value="SMRTER, ISOFORM G"/>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
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        </location>
    </feature>
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            <end position="891"/>
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    </feature>
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    <feature type="region of interest" description="Disordered" evidence="1">
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="1122"/>
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        </location>
    </feature>
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            <begin position="1195"/>
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        </location>
    </feature>
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        <location>
            <begin position="36"/>
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        </location>
    </feature>
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        </location>
    </feature>
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            <begin position="1200"/>
            <end position="1215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAR23936.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1215" mass="134808" checksum="BBEE7F151A3BC03D" modified="2009-07-28" version="1">MAPLNRVGEKKPYQYTNADPRHMPYSRTSGTLPAGSTARSAPSESAPPHSSGSVHRPSRYNPSSTSRPHTPFGTVHSKPRTGSRYNPEPGFVQSGSRGSSETRRFPNSPNAPSSSRYSFQAAAPIPKASHHSYYNPQYSRRRPSSATDGYDPASHGYTARSKWKEPYGSASGAAYDGRMSPSNTSPVPPLSKPPYYNRYHGTTVHQNIHAQPYSSRYGHYRKEHSSQDSSYTTSNKFPLYENEHSLGSSLINSVPQTSRKTEERVSNNHERIDLQKTVNDDSVKREEPSSLNTTPSDTKSGQTDNADENNAESAPKEEEAVYNKPGKHDIIRKAHSIEDFNEAHPSQPTSSSDDQGAAGSTDTYFAAKPVEKPSHLPSRHSSEAAQGYTQPLNEVESCIFPLPEAETKLWELKNRDRESIVKNQKYLLKSPVTCLQEYPFMSQNILVHEQAVRPILVKSLSKLQDYERLRKLQLKWQFLKLDEKWTKNCQMLEAVSEEVKKVDHATTDSTEEESQFSKSKEKEEREQQSRASSRRRNRADFVDDNEIENVLLRIDPDYKHHQLAAKIPPMIIDPVKKFAVKYKDVNNLVTDKNKWATRVLRDGIDTFTSAEHDLFVEGYLAYPKRFGKISQFMGGLRKPQECVLHYYKTKKETNYKQLLVEKNKRRKINAGRRRREKDKEKTSAAVPEQSGQEAEEMSERNDSNFTEKERTMDETEDEEVDQGAQIKEAAFGPAEKVEDTSIVSTGSNVALPDGPRGLVQQQQEEVSAQDLSMENEEDTESKKRKIQEIEEVTNDLPNLQPIAPAFASEDKDATDIDESRRPFKADDNEKVQARKKTKHSDGYHKSSYWSVKETNLFPELLREYGTQWALISERLGTKSTTMVRNYFQRNADQMGWQALVESSNSESPENKLNTQFGNSDATPGAYDTVPMQQAPTVSIFSQIHRDAATPMQLPIASVSAMDSFSQQSTPQGLPPPRLPSIQLGLSTDQAERKPLLLSDSHSSNSAHAPEAMPSVHFAPSVAINNSSCPSPATSSQAGISSRRSSIKNLLNNDDHTDSNSAWSKQRAVELPPIAFPEVNATAPVVVQELRSSNFTSDTAAPSPSSNLPPKQPSFLNSILNAASAPASQSPHVPTGTAPRTRYSVSSATRPILPPVGLSTATAPTPARPREFNFANDPLAALAAVASAPEALVSLIPQDGGASNSSTQQKPSNTGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKN8</accession>
    <name>C5DKN8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0F06226p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0F06226g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="CAR24039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_002554430.1"/>
    </dbReference>
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        <property type="interactions" value="20"/>
    </dbReference>
    <dbReference type="GeneID" id="8292675"/>
    <dbReference type="KEGG" id="lth:KLTH0F06226g"/>
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    <dbReference type="HOGENOM" id="CLU_083988_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKN8"/>
    <dbReference type="OMA" id="HANSIHT"/>
    <dbReference type="OrthoDB" id="4035860at2759"/>
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        <property type="component" value="Chromosome F"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="26"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="29"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="191" mass="21165" checksum="F5B242CBE884554C" modified="2009-07-28" version="1">MSESHHDNAFQERKRKLSVWVKRIIQPNREQAQTQQHQRTQRQERRRPQQFAVAHSKPHSKRRSVKGGLASAELTTPQAKREPLARSVAWGDPIYKEAIPHLPRLEFDHIQDNASTAPLVSHCSSSAKSSVFSDVTSLQSTRATVSSSKTFDTNASTVGIPSASILDRARHTNPAQGAPSVFSAATTQHTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKQ6</accession>
    <name>C5DKQ6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Dipeptidyl peptidase 3</fullName>
            <ecNumber evidence="11">3.4.14.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Dipeptidyl aminopeptidase III</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Dipeptidyl peptidase III</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0F06666g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>Release of an N-terminal dipeptide from a peptide comprising four or more residues, with broad specificity. Also acts on dipeptidyl 2-naphthylamides.</text>
            <dbReference type="EC" id="3.4.14.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11 13">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11 13">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the peptidase M49 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.14.4" evidence="11"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554494.1">
        <property type="nucleotide sequence ID" value="XM_002554448.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKQ6"/>
    <dbReference type="FunCoup" id="C5DKQ6">
        <property type="interactions" value="674"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKQ6"/>
    <dbReference type="MEROPS" id="M49.004"/>
    <dbReference type="GeneID" id="8292695"/>
    <dbReference type="KEGG" id="lth:KLTH0F06666g"/>
    <dbReference type="eggNOG" id="KOG3675">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011977_1_0_1"/>
    <dbReference type="InParanoid" id="C5DKQ6"/>
    <dbReference type="OMA" id="QRYWIRD"/>
    <dbReference type="OrthoDB" id="4694525at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008235">
        <property type="term" value="F:metalloexopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.540.30:FF:000001">
        <property type="entry name" value="Dipeptidyl peptidase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.30">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005317">
        <property type="entry name" value="Dipeptidyl-peptase3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039461">
        <property type="entry name" value="Peptidase_M49"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23422:SF11">
        <property type="entry name" value="DIPEPTIDYL PEPTIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23422">
        <property type="entry name" value="DIPEPTIDYL PEPTIDASE III-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03571">
        <property type="entry name" value="Peptidase_M49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007828">
        <property type="entry name" value="Dipeptidyl-peptidase_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="5 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 11 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="6 11" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 11 13" id="KW-0862">Zinc</keyword>
    <feature type="active site" evidence="12">
        <location>
            <position position="468"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="467"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="472"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="525"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR007828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR007828-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR007828-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR24057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="719" mass="81217" checksum="BE5EA8F62FEFCC9D" modified="2009-07-28" version="1">MLRTLKSGLSRALRSAKGTKPYSKSSIMAKSTYLADHKAPLVMLSAKDFFAQLSRDEKFYAHYMSKASHAGSRVVLRQVSHESESIYDLIKTIHEKLKGTYPEGEETDLYLEYVSQFLSNLGNYKSFGDFKFVPRCSESFIMGLLELAGIDKNSKPSNKSCPYKTWADVVSKGIFHVDEKCALLGFPSEGHCSSYYFGDVSPEDMRLLKDEVFGHLKILPENTRIKKLSSNNFEILVASKVSNYTLPEYPKSPVKLSNGAEVIFVFGDHSREMGLIADYLRKARDYAANDTQKSMLDAYIKHFETGSSHAHKDSQKLWVKDLSPVIETNVGFIETYREPSGIIGEFESLVAIQNKERTQKFATMVQNAGKFISLLPWDKEFEKPKFQPPDFTSLEVLTFAGSGIPAGINIPNYDDVRLNIGFKNVSLGNILSSSLGSKMPITFVTDDDRKILEKFQSDSFEVQVGIHELLGHGSGKLLSELDDGTFNFDKENPPLGLEGKPVTTYYKKGETWGSKFGQLAGAFEECRAEVIAMYLITNRELLEIFGFKTQEDQDNIIYAGYLQMARAGLLALEFWDPSTKKWGQPHMQARFSIMKTFLKHSGDKGFLTLSHSEPDFSDLKIHLNRSMIENVGRNAVRDYLTHLHIYKSSGDVEQGSKYFVERSTVDPELAQFRSIVIEKKLPRRQFIQANTVIEKGDVSLKEYDETSVGMLQSYLEREV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKZ2</accession>
    <name>C5DKZ2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Palmitoyltransferase</fullName>
            <ecNumber evidence="11">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0F08668g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DHHC palmitoyltransferase family. ERF2/ZDHHC9 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="11"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554580.1">
        <property type="nucleotide sequence ID" value="XM_002554534.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKZ2"/>
    <dbReference type="FunCoup" id="C5DKZ2">
        <property type="interactions" value="188"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKZ2"/>
    <dbReference type="GeneID" id="8292786"/>
    <dbReference type="KEGG" id="lth:KLTH0F08668g"/>
    <dbReference type="eggNOG" id="KOG1311">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047581_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKZ2"/>
    <dbReference type="OMA" id="YVTMFLI"/>
    <dbReference type="OrthoDB" id="9909019at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF43">
        <property type="entry name" value="PALMITOYLTRANSFERASE APP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="79"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="220"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="253"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="13">
        <location>
            <begin position="175"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR24143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="358" mass="40591" checksum="DBFBF465B75D85D6" modified="2009-07-28" version="1">MSERNLQEGHHRSPTNGECKVPTQEPNAQPSWWRDCLYWLVTFEARETRKSNYSSMPKVVNYNFFLGGRLRAVSGTEPLSLFVLIALLAPMVLYSIFEAQALWRLGRGHGALVILFYYFWAICLVSFIKTATSDPGVLPRNVHVPIVGEEFQLPRSYYNIITLPSAHPEGKTVDVKYCATCRIWRPPRASHCSTCEACVLTHDHHCTWVNNCIGQRNYRYFLTFLASCCLATTLCIVGCGIRVAQATRPDRVVVAILLIIYCALGLCYPLLLLVYHMFLTSTQQTTREYLKHVPSKTAIRQALSSPNANPFEKGNRIKNMISLICQRRGVSVMSARESHSAGDWRFVSLPQPHSFEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DL37</accession>
    <name>C5DL37_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0F09680p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0F09680g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554625.1">
        <property type="nucleotide sequence ID" value="XM_002554579.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DL37"/>
    <dbReference type="FunCoup" id="C5DL37">
        <property type="interactions" value="362"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DL37"/>
    <dbReference type="GeneID" id="8292832"/>
    <dbReference type="KEGG" id="lth:KLTH0F09680g"/>
    <dbReference type="eggNOG" id="KOG2450">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_0_0_1"/>
    <dbReference type="InParanoid" id="C5DL37"/>
    <dbReference type="OMA" id="ILMGAWK"/>
    <dbReference type="OrthoDB" id="310895at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046394">
        <property type="term" value="P:carboxylic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006598">
        <property type="term" value="P:polyamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000001">
        <property type="entry name" value="Aldehyde dehydrogenase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000026">
        <property type="entry name" value="Aldehyde dehydrogenase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000012">
        <property type="entry name" value="Betaine aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43720">
        <property type="entry name" value="2-AMINOMUCONIC SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43720:SF2">
        <property type="entry name" value="2-AMINOMUCONIC SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="2 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="6">
        <location>
            <begin position="28"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR24188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="502" mass="54532" checksum="754FE9C63E220000" modified="2009-07-28" version="1">MSLFTEISIPQLNISYKQPVGLFINNKFVESSENEQIETVNPATGEAITKFHAASEADIDKAVAAAREAYESTWSKTSPEERGVLLGKLGQLIEQEKELLAAIETVDSGKPYYTNALGDLEQILQLTRYYAGAADKFTQGKLLPVSSDKYAYTVKAPYGVVAQVVPWNYPLAMASWKMQGCLAAGNTIVIKPAENTSLSLLYFAQLVEKAGFPPGVVNVVPGYGGVAGSRLAAHPDVDKIAFTGSTAVGQKVMAAAAMSNLKAVTLECGGKSPALVFEDANLDEAVKWTAAGIYYNSGQNCTANSRIYVQESVYEKFLLKFTAHVQETWKFGKTSDLFDQECTVGPVVSKTQYERVQGYIEHGKSTEKLSLKQMGQPSDSKGYFITPTIFINVPQDSKLNQEEIFGPVAVIAKFKDYKEGIALANDTSYGLASAVFTEDIRKANYFARDIKAGTVWINSSNDEEVSVPFGGFKMSGIGRELGESGMDAYLQTKAVHVNISKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLH8</accession>
    <name>C5DLH8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0G00836p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0G00836g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the APC10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555076.1">
        <property type="nucleotide sequence ID" value="XM_002555030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLH8"/>
    <dbReference type="FunCoup" id="C5DLH8">
        <property type="interactions" value="773"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLH8"/>
    <dbReference type="GeneID" id="8293334"/>
    <dbReference type="KEGG" id="lth:KLTH0G00836g"/>
    <dbReference type="eggNOG" id="KOG3437">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039415_2_0_1"/>
    <dbReference type="InParanoid" id="C5DLH8"/>
    <dbReference type="OMA" id="WVALTFE"/>
    <dbReference type="OrthoDB" id="24948at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070979">
        <property type="term" value="P:protein K11-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08366">
        <property type="entry name" value="APC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016901">
        <property type="entry name" value="APC10/Doc1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004939">
        <property type="entry name" value="APC_su10/DOC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12936">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12936:SF0">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03256">
        <property type="entry name" value="ANAPC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01337">
        <property type="entry name" value="APC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51284">
        <property type="entry name" value="DOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="DOC" evidence="6">
        <location>
            <begin position="53"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR24639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="242" mass="27371" checksum="8AF6049834AC1A5D" modified="2009-07-28" version="1">MLDRWQGLVPDAEGRSFPVKPNQYLVLDDKQEDFDSNGVTYIGRPSEEYDDEVATEAYLERYTQGLELMSSQGHIDITNLAYWKASSQKDGNPVSQALDDNPTTFWQSDGLQPHKIEISFSKRVDIVQFALFLSLVVDESYTPQIIKIYAGHSASDATLYKTLEVRNVNCWVALTFEDNRPEDNLLKCQYIRMIIPVNHENGKDTHLRGARIYALSLKPPVDNSGVLDSFPMGTLLSGFSVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLK4</accession>
    <name>C5DLK4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0G01408p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0G01408g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555102.1">
        <property type="nucleotide sequence ID" value="XM_002555056.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLK4"/>
    <dbReference type="FunCoup" id="C5DLK4">
        <property type="interactions" value="48"/>
    </dbReference>
    <dbReference type="GeneID" id="8293360"/>
    <dbReference type="KEGG" id="lth:KLTH0G01408g"/>
    <dbReference type="eggNOG" id="ENOG502SBF7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_446925_0_0_1"/>
    <dbReference type="InParanoid" id="C5DLK4"/>
    <dbReference type="OrthoDB" id="4068191at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="418"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="446"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="481"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="507"/>
            <end position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="611" mass="66108" checksum="4CA45FDE0C4CD231" modified="2009-07-28" version="1">MCHGIGIRHYVRASVCHFARAARVARASSPNCRCTTSTSTSSHGQQNPDPPAAMATTADALFREKPVGELRNYRLQLSHDIAKAKDRFETELGRRYTDMLAVTDQVDELLQQARTADADLMELCFNDAKFKLAALPDPAAFHAAAPAAATADPELGPGAQPDLAGIEATLAVSEWVLAVKDFAREPALQKHFDSLVQGFHTVLSLHDVSRYASVLARNCRALEDTILEQQAEFSASQWVKLHRLVHSHAEVFPFKRVDELDALVFSFLLANEEPLRAAGVDADVQAFLEGEPFKLKFVERVLQDVDAEFQALEEAAAGSAPTPELYRMNFDDGSLLALVTEANLYCSGAISPRDRALYALVENITALVRKLQRSQADSAVISKVKERLSEVLIERRKEVVEAPSDNSVLQLEALQKKGDTEFGTSQQGDAGPELKHKDAPLDSVTAAQKGESTDVVGNAARKTPVVSTDAEAEAHDSDTGASSTETEAPGAETETTSAETEVVTPSAETETQTPSAETETQTPSAETETQTPSAETETQTPSAEAEATSAQTQPPSAENNNYEKTMHKGNIWQPVESLPLTRDIVRAAVQKLNTDNLLAYLGGQIAQVDSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLM3</accession>
    <name>C5DLM3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">KLTH0G01826p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0G01826g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555121.1">
        <property type="nucleotide sequence ID" value="XM_002555075.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLM3"/>
    <dbReference type="FunCoup" id="C5DLM3">
        <property type="interactions" value="1177"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLM3"/>
    <dbReference type="GeneID" id="8293379"/>
    <dbReference type="KEGG" id="lth:KLTH0G01826g"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1086140_0_0_1"/>
    <dbReference type="InParanoid" id="C5DLM3"/>
    <dbReference type="OMA" id="LWGHKLG"/>
    <dbReference type="OrthoDB" id="654211at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000433">
        <property type="term" value="P:carbon catabolite repression of transcription from RNA polymerase II promoter by glucose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000018">
        <property type="entry name" value="Krueppel-like factor 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000072">
        <property type="entry name" value="zinc finger protein 143 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051007">
        <property type="entry name" value="creA/MIG_C2H2-ZnF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47428">
        <property type="entry name" value="REGULATORY PROTEIN MIG1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47428:SF1">
        <property type="entry name" value="REGULATORY PROTEIN MIG1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="13"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="41"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="56"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="251"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR24684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="307" mass="32434" checksum="22F13548CC80D85A" modified="2009-07-28" version="1">MGVAQQESDHRPFKCDMCGKGFHRLEHKRRHIRTHTGEKPHACNFPGCVKRFSRSDELKRHVRTHMSTSRGKSRKRSGSSPALEHPVVVHQLPPISVPGVAQMPPLVPVAQLASVAQLASVAAPVAVAPSATAYIPRAGPSSSTSLLIPGVVAPMVSPAVSRAVSPAVSPAFSPHTSSIHLHPGHASFLSSSSSSMSSVFSGGESGLASPLPSSPDLRVHKLAGLLPSVQKSTRSASGGMSIASAVLNNAKRQQPLPAARPRLQGRNSQRAKFELNDDDDSDSSQPADVRLPPLSSMLDGIQAFQER</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLQ6</accession>
    <name>C5DLQ6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">26S proteasome regulatory subunit 6B homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0G02662g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555154.1">
        <property type="nucleotide sequence ID" value="XM_002555108.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLQ6"/>
    <dbReference type="FunCoup" id="C5DLQ6">
        <property type="interactions" value="1410"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLQ6"/>
    <dbReference type="GeneID" id="8293417"/>
    <dbReference type="KEGG" id="lth:KLTH0G02662g"/>
    <dbReference type="eggNOG" id="KOG0727">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_2_0_1"/>
    <dbReference type="InParanoid" id="C5DLQ6"/>
    <dbReference type="OMA" id="QDIGGMD"/>
    <dbReference type="OrthoDB" id="10255768at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010604">
        <property type="term" value="P:positive regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070682">
        <property type="term" value="P:proteasome regulatory particle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080090">
        <property type="term" value="P:regulation of primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000020">
        <property type="entry name" value="26S protease regulatory subunit 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000046">
        <property type="entry name" value="26S protease regulatory subunit 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000033">
        <property type="entry name" value="26S protease regulatory subunit 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032501">
        <property type="entry name" value="Prot_ATP_ID_OB_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16450">
        <property type="entry name" value="Prot_ATP_ID_OB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0647">Proteasome</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="13">
        <location>
            <begin position="202"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="48"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00068703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR24717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="425" mass="47074" checksum="3C13616A30A55032" modified="2009-07-28" version="1">MEELGILSTPTSGSGETAPIGSYGSLVAQLNSNAGSAINGSSDNYFRLKKLEKELELLNLQEDYIKDEQRHLKRELLRAQEEVKRIQSVPLVIGQFLEPIDENTGIVSSTTGMSYVVKILSTLDRELLKPSTSVALHRHSNALVDILPPDSDSSISIMSANEKPDVSYSDVGGLDMQKQEIREAVELPLVQADLYQQIGIDPPRGVLLYGPPGTGKTMLVKAVANSTNVAFIRVNGSEFVHKYLGEGPRMVRDVFRLARENAPSIIFIDEVDSIATKRFDAQTGSDREVQRILIELLTQMDGFDQSINVKVIMATNRADTLDPALLRPGRLDRKIEFPSLRDRRERRLIFGTVAAKMALAPEVDLDSLIIRNDPLSGALIAAIMQEAGLRAVRKNRYVILQSDLEEAYASQVKSSSDVDKFEFYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>C5DM38</accession>
    <name>C5DM38_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0G05720p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0G05720g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sirtuin family. Class I subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555286.1">
        <property type="nucleotide sequence ID" value="XM_002555240.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DM38"/>
    <dbReference type="FunCoup" id="C5DM38">
        <property type="interactions" value="94"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DM38"/>
    <dbReference type="GeneID" id="8293553"/>
    <dbReference type="KEGG" id="lth:KLTH0G05720g"/>
    <dbReference type="eggNOG" id="KOG2684">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021544_4_0_1"/>
    <dbReference type="InParanoid" id="C5DM38"/>
    <dbReference type="OMA" id="THKFIAH"/>
    <dbReference type="OrthoDB" id="2919105at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017136">
        <property type="term" value="F:histone deacetylase activity, NAD-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01407">
        <property type="entry name" value="SIR2-fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1600.10">
        <property type="entry name" value="SIR2/SIRT2 'Small Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050134">
        <property type="entry name" value="NAD-dep_sirtuin_deacylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003000">
        <property type="entry name" value="Sirtuin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026591">
        <property type="entry name" value="Sirtuin_cat_small_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026590">
        <property type="entry name" value="Ssirtuin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085:SF8">
        <property type="entry name" value="NAD-DEPENDENT HISTONE DEACETYLASE HST3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02146">
        <property type="entry name" value="SIR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50305">
        <property type="entry name" value="SIRTUIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Deacetylase sirtuin-type" evidence="7">
        <location>
            <begin position="39"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="366"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="451"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="366"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="471"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="486"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="194"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR24849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="497" mass="56203" checksum="A9203C6EEF157C19" modified="2009-07-28" version="1">MPCLILSQESFDEKVPSSLSKRVANRPDLKTSKLRHLSITEPSEKDKELLKQVNKQISKSRKILVLTGAGISCNAGIPDFRSSSGCYELVKQEYPDAAIRSGQEMFDISLFREEQKICIFATFMEKLYSSARVAQPTRTHRFIAHLKNRGKLLRCYTQNIDGLEENLGLEMSSERDCDTSMANQWRNLDVVQLHGDLNRLSCTQCFKTFNWNRGLSRCLRSGQLPSCPRCEDSSTQRSLMGKRNTGSIGLLRPNIVLYGENHPSCEFITQGLNLDIARGRPDLFFIMGTSLKVDGVKKLVRNFSKQVHDRNGLVVLINKTRIGDCNWHGIIDYQILSDCDAWVDHLKEDIPEFFKTQQQVEKARQLKRENSILRKRQREERKLTPPSTPSRKKATAQKSVKSEIHKSNDGASTPEDSCPTLFTKGERTSQESVLLKLQIEDQKSEPLSLLDASSLPSLGGDNSMSNHTDTTIEDDPTDIDEDLPISSYSTLSRKQTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>C5DM73</accession>
    <name>C5DM73_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutaredoxin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0G06534g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutaredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555321.1">
        <property type="nucleotide sequence ID" value="XM_002555275.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DM73"/>
    <dbReference type="STRING" id="559295.C5DM73"/>
    <dbReference type="GeneID" id="8293590"/>
    <dbReference type="KEGG" id="lth:KLTH0G06534g"/>
    <dbReference type="eggNOG" id="ENOG502SC8X">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125054_0_0_1"/>
    <dbReference type="InParanoid" id="C5DM73"/>
    <dbReference type="OMA" id="SDVWDKR"/>
    <dbReference type="OrthoDB" id="429967at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008554">
        <property type="entry name" value="Glutaredoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052565">
        <property type="entry name" value="Glutaredoxin-like_YDR286C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33558">
        <property type="entry name" value="GLUTAREDOXIN-LIKE PROTEIN C5ORF63 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33558:SF1">
        <property type="entry name" value="GLUTAREDOXIN-LIKE PROTEIN C5ORF63 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05768">
        <property type="entry name" value="Glrx-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0249">Electron transport</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="108" mass="12219" checksum="10453FA2CD0BEA4F" modified="2009-07-28" version="1">MLFIRRFHAASALANYAHVNLTLFSKANCGLCDKAKGVLSQVLAEKPYADIGVKIIDIDEPQNKEWWTKYCLDVPVLHIENTKNPGSLSKIFHKLDVDQVKHQVQDSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DM97</accession>
    <name>C5DM97_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">KLTH0G07062p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0G07062g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Proton-conducting pore forming of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments.</text>
    </comment>
    <comment type="function">
        <text evidence="9">Proton-conducting pore forming subunit of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10 11">V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'', d, e, f and VOA1). The decameric c-ring forms the proton-conducting pore, and is composed of eight proteolipid subunits c, one subunit c' and one subunit c''.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the V-ATPase proteolipid subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555345.1">
        <property type="nucleotide sequence ID" value="XM_002555299.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DM97"/>
    <dbReference type="FunCoup" id="C5DM97">
        <property type="interactions" value="268"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DM97"/>
    <dbReference type="GeneID" id="8293614"/>
    <dbReference type="KEGG" id="lth:KLTH0G07062g"/>
    <dbReference type="eggNOG" id="KOG0233">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085752_0_1_1"/>
    <dbReference type="InParanoid" id="C5DM97"/>
    <dbReference type="OMA" id="TSPYMWG"/>
    <dbReference type="OrthoDB" id="10264021at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18177">
        <property type="entry name" value="ATP-synt_Vo_c_ATP6F_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18178">
        <property type="entry name" value="ATP-synt_Vo_c_ATP6F_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.610:FF:000002">
        <property type="entry name" value="V-type proton ATPase proteolipid subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.610">
        <property type="entry name" value="lithium bound rotor ring of v- atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002379">
        <property type="entry name" value="ATPase_proteolipid_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000245">
        <property type="entry name" value="ATPase_proteolipid_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035921">
        <property type="entry name" value="F/V-ATP_Csub_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10263">
        <property type="entry name" value="V-TYPE PROTON ATPASE PROTEOLIPID SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00137">
        <property type="entry name" value="ATP-synt_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00122">
        <property type="entry name" value="VACATPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81333">
        <property type="entry name" value="F1F0 ATP synthase subunit C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7 11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="57"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="100"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="144"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="183"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="12">
        <location>
            <begin position="61"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="12">
        <location>
            <begin position="145"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00046480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR24908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="212" mass="22394" checksum="E25E3F81B4D1FD62" modified="2009-07-28" version="1">MSSKSESSMGISLPSFRQWLRLLVITVAVWYAGKKLFTGHGEDINFGRFLSKTSPYMWANLGICMCIGLSVVGAAWGIFITGSSIIGAGVRAPRITTKNLISIIFCEVVAIYGLIMAIVFSSKLTVASKETLFSKSNLYTGYSLFWAGLTVGLSNLICGVAVGITGATAAVSDAADSSLFVKILVIEIFGSVLGLLGLIVGLLMAGKAVEFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMF3</accession>
    <name>C5DMF3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">KLTH0G08404p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0G08404g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the OST3/OST6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555401.1">
        <property type="nucleotide sequence ID" value="XM_002555355.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMF3"/>
    <dbReference type="FunCoup" id="C5DMF3">
        <property type="interactions" value="296"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMF3"/>
    <dbReference type="GeneID" id="8293674"/>
    <dbReference type="KEGG" id="lth:KLTH0G08404g"/>
    <dbReference type="eggNOG" id="KOG2603">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052855_1_0_1"/>
    <dbReference type="InParanoid" id="C5DMF3"/>
    <dbReference type="OMA" id="VLFGMYS"/>
    <dbReference type="OrthoDB" id="67566at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008250">
        <property type="term" value="C:oligosaccharyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018279">
        <property type="term" value="P:protein N-linked glycosylation via asparagine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021149">
        <property type="entry name" value="OligosaccharylTrfase_OST3/OST6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12692">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12692:SF0">
        <property type="entry name" value="GH11935P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04756">
        <property type="entry name" value="OST3_OST6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0732">Signal</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950546" evidence="10">
        <location>
            <begin position="21"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="178"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="210"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="265"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="305"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR24964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="347" mass="38586" checksum="614B852620020D70" modified="2009-07-28" version="1">MRFTQLAVFFAAFFLPFISAISNEKLSKEASRYKGIIQLTNNNFKRILDSKREAYIVVLLTATNAQVGCTLCMELDPEFSMMADSWYKDHPEGLSESGTGMFFARADFDPKKNSDVFMHFKVNNVPRLLFLTPQGDPSSFSQINLPGEGGMTRVLAIVNTLRDATGISDFKIYQPINWGSVFVTAFATAAVTLLVKKYKPVAAKLITSKYVWGLATVFVIILWNAGYMFNSIRGSQFAGMTQDGSAVIYFMEGQQQNQFGIETQIVGVIYGVLASCVAGLVLFVPYAKQFYATDKSGRPTLSKACFVEFSLTLSFLVTLYLVYGALTGVFKLKSPGYPFKLIKFPFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMK1</accession>
    <name>C5DMK1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0G09592p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0G09592g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein-tyrosine phosphatase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555449.1">
        <property type="nucleotide sequence ID" value="XM_002555403.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMK1"/>
    <dbReference type="FunCoup" id="C5DMK1">
        <property type="interactions" value="33"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMK1"/>
    <dbReference type="GeneID" id="8293727"/>
    <dbReference type="KEGG" id="lth:KLTH0G09592g"/>
    <dbReference type="eggNOG" id="KOG1572">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047845_3_1_1"/>
    <dbReference type="InParanoid" id="C5DMK1"/>
    <dbReference type="OMA" id="MPLNYSF"/>
    <dbReference type="OrthoDB" id="6375174at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052840">
        <property type="term" value="F:inositol diphosphate tetrakisphosphate diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000035">
        <property type="entry name" value="Tyrosine phosphatase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004861">
        <property type="entry name" value="Siw14-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126:SF74">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE-LIKE PROTEIN OCA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03162">
        <property type="entry name" value="Y_phosphatase2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR25012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="169" mass="19792" checksum="2A9B8D3582A04892" modified="2009-07-28" version="1">MVYIPPLNFAPVVSTEVSLYRSGYPMPLNYAFIADQLHLRTIIYVGDKELSEDYNEFLTQHNIQYHFVHMNSCRDKNVQEQMDKVLRLIVDRANYPILIHSNKGKHRVGVVVGIIRKLLQGWSTTGIYQEYDIFSGGLKGQVDLEFITMFDTELIVRRDHLPDFVKWER</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMK3</accession>
    <name>C5DMK3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0G09636p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0G09636g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555451.1">
        <property type="nucleotide sequence ID" value="XM_002555405.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DMK3">
        <property type="interactions" value="163"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMK3"/>
    <dbReference type="GeneID" id="8293729"/>
    <dbReference type="KEGG" id="lth:KLTH0G09636g"/>
    <dbReference type="eggNOG" id="KOG3630">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_250354_0_0_1"/>
    <dbReference type="InParanoid" id="C5DMK3"/>
    <dbReference type="OMA" id="NIYTWRI"/>
    <dbReference type="OrthoDB" id="248320at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039462">
        <property type="entry name" value="Nup159/Nup146_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16755">
        <property type="entry name" value="Beta-prop_NUP159_NUP214"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117289">
        <property type="entry name" value="Nucleoporin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950434" evidence="5">
        <location>
            <begin position="26"/>
            <end position="1599"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin Nup159/Nup146 N-terminal" evidence="6">
        <location>
            <begin position="97"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="494"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1069"/>
            <end position="1241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1265"/>
            <end position="1284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="503"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="550"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="573"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="625"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="669"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="703"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="758"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="779"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="798"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="832"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="849"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="889"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="929"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="954"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="973"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="989"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1018"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1087"/>
            <end position="1107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1110"/>
            <end position="1142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1144"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1165"/>
            <end position="1178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1206"/>
            <end position="1216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF16755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR25014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1599" mass="173078" checksum="EC5C1CB9A6E4C163" modified="2009-07-28" version="1">MRRCLPTRVCPVYIQPLFILCLCACTPPRALRLIFFFNSHNKVIASHHQYKKENLRPDLCTLMSVLGDELPTATSEDFGFKPLGKIDILPSYEEQLPFSKLQNLAISNISKLYAAATNQKVVVGTLHNLRSQLPDAAGTDADASEEPRQLQFLSEKDLECVVYVGFNSAGTAALCITKDFGLFRFDVSQKEWSSLNISLPTSQELTVTSVKPLSPDLQILLVQASNQLYQVEISGASSVIANDITDFDVGHNQYVLMHGDGTIEIHKDFQEQSPKTLTPPDDVLEQISEECVPLAIEVLSELRYLVVFGNPVTESDEDVMYDHRMFLVTLTGNNEAKFQESFDIAPAFGSVLRTPNYYRISLNQLMPSLPHLYVISSAASSELTLLDDNAVIQPNQDSDRAVLPINQDTDNDTNPVGMAVDLTSELQVAEPCQGVNVSNNLPIVFVLNSEGELLVFALFNSSGIKKNEFTAEGAFNALQSEWKASEEMLSIKLKTSTDKETGSSETSGGSRPPTDSSTGTSIEISGSDDSSITAQPSSPSIKSSSPQPFEGQQKSSPFTGQLASEQSSFGKPSFGQPSFGQSSSGQSSFGQSSYGQTSFGKSSLGQSSFGQPSFGQSSFGQSASEKSTFGQPSFGQTAFGKPSLEQPVAGQQAFGKPSFGQPAFGQSVFGKSSVDSSATKPAQSLESKTPALGSDREAVSGAKDTSSQLTQTKPLFGNTTFGQSTPTKPLTDSSMSTAGAGSFGKPAFGKPAFGSGFQFGTSNPQINSDGSKDTASSQPAFKAPAFGAPAFGTPAFGQTSIGSHESKPQVTGTENSTGSGFGAFSAFAKTDSPFSKLSSGPSPFINKPVADKPESNESEGKETLKSKPDESSSIGSEHGFGIPSKSLFSKTAISNSSSVNSSASKSDSKDNASSLFSNFNDKIAEHKQSQSPFSQFQNQPVPSATNNEESTNDDESHQAEDSDSNSDKETSTGDDASSTESSEDAQSQPEHDEQLKLTKEGDSGNSDQFSKEQHSLEPGSNKTDFSSLTERIKKVANIDGNSFSDAFSKSMTVKEPEASQTGASAFSKFSKGLQKSPKPAFSFANIGKQQSLNTDSKLTNSSNNLGRSTLGERDTEKDSKVNRKESQDSKKSEHQSAAKEEGSEATTTFNKSEGSFDQKGANEAVLREGKTLSGKETDELGESDTWKPSAAVSQKETTDEYCSSEVDSKEASKEVSEESPVNLPDTEYPGKRTETSTEGESFDDLDDLKEELEHIKTNTDKIKMPTSSEHATEPAHTNPRFALGNSSTETFDAKTQTFRQVHDAEVGTEKNAVDHADVQAFENDEKYLAQQYAPKKIGTFFIGAKLLQKPSLSKNETMKRIEATFNVVSSELEVLEGNLKNIGEFINDQSKNPFSRTAETLFLNYTWRLEEASVLNSFVKELFEEGGPTFAEVSSLMCDAENLRDKDLVSLKSNQFSTREYFCQLKTLCEQNETFKRGLTFRQTQFQRKLRSKISSVQNLIHNMGGTLRVLQAQASTGDVKETPLVSRLIESSSRRGDLLEAIQALRSEVAELKIRDRSLIKFSDADSATRTAPISELKLKLDTKQQIGQVLMNRLQNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMZ3</accession>
    <name>C5DMZ3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">RNA exonuclease 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0G12870g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">3' to 5' exoribonuclease required for proper 3' end maturation of MRP RNA and of the U5L snRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the REXO1/REXO3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555591.1">
        <property type="nucleotide sequence ID" value="XM_002555545.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMZ3"/>
    <dbReference type="FunCoup" id="C5DMZ3">
        <property type="interactions" value="106"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMZ3"/>
    <dbReference type="GeneID" id="8293874"/>
    <dbReference type="KEGG" id="lth:KLTH0G12870g"/>
    <dbReference type="eggNOG" id="KOG2248">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022453_5_4_1"/>
    <dbReference type="InParanoid" id="C5DMZ3"/>
    <dbReference type="OMA" id="IDCEMGF"/>
    <dbReference type="OrthoDB" id="3996471at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010629">
        <property type="term" value="P:negative regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06145">
        <property type="entry name" value="REX1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000031">
        <property type="entry name" value="RNA exonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034922">
        <property type="entry name" value="REX1-like_exo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047021">
        <property type="entry name" value="REXO1/3/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Ribonucl_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801:SF118">
        <property type="entry name" value="RNA EXONUCLEASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801">
        <property type="entry name" value="RNA EXONUCLEASE REXO1 / RECO3 FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Exonuclease" evidence="12">
        <location>
            <begin position="231"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR25154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="392" mass="43925" checksum="7D21319CC95A3319" modified="2009-07-28" version="1">MAVLRPVDLKTQPAPYQSRYMVLQKVLKTLEKFHAASPELGKKAVEVEASVAKKSASSQSYRFNASVVLRDILKSKGKLDCLEPSSKKKGVIASAAKLTKSQAMETLQAVLVDQATLVANGYNTGAPEKTEQMSDTDVQGVYTTCIRCNTKFRKDQIMNPTTCRFHVQRKKFNRETRQGEYACCGETTNSSSFLALGCKTLFHHVFRPETFSEMERISPFRNTCKVQGKANVLALDCEMAFTSCGYELIRLTIVDFFTSKVLYDEIVRPFGEVIDLNSEFSGVHVIKEETSVSFDEMLEKILHESLINKNSILIGHGLENDLNVMRLIHDKIIDTAILYPRGHYKSSLKDLAFEVVSRRIQTGEHDSSEDAIATMSVLKSKLGIPLAQDVWE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2010-10-05" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>C5DN25</accession>
    <name>GEP5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName>Genetic interactor of prohibitin 5, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Required for respiratory growth protein 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">GEP5</name>
        <name type="synonym">RRG5</name>
        <name type="ordered locus">KLTH0G13596g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Essential for respiratory growth and required for maintenance of mtDNA. Required for cell survival in the absence of prohibitins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GEP5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555623.1">
        <property type="nucleotide sequence ID" value="XM_002555577.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DN25"/>
    <dbReference type="FunCoup" id="C5DN25">
        <property type="interactions" value="34"/>
    </dbReference>
    <dbReference type="GeneID" id="8293906"/>
    <dbReference type="KEGG" id="lth:KLTH0G13596g"/>
    <dbReference type="eggNOG" id="ENOG502S2Q8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079415_0_0_1"/>
    <dbReference type="InParanoid" id="C5DN25"/>
    <dbReference type="OMA" id="FWPYERH"/>
    <dbReference type="OrthoDB" id="4066262at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031455">
        <property type="entry name" value="Gep5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17053">
        <property type="entry name" value="GEP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0496">Mitochondrion</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000399686" description="Genetic interactor of prohibitin 5, mitochondrial">
        <location>
            <begin position="1"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="267" mass="31397" checksum="73897C19FD2CCB52" modified="2009-07-28" version="1">MNMAVRALEKLPLDIRTLELLERKLLKKSLPGFQELAQQYTADGSSKSLESIVHLTYFHWCNEDAPHIKKFREHYAELRDHWPYEFHRSILSMDTPKTQSIRFLWSSNSPKVLSKMSYEKYAHDTEERPSRLTELQRTVQFHEVFQHFLFLKSRPHLCKTRKGLAVPIVEIPMKPLGQNIPEVRVRNLFKRKIAYVWKVLALDSPALSRQNELSLAKIIDSPQLPEDVSSKRELKRLYQRACRGAYTIEQHPVLGLEIKESKLLKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>C5DN79</accession>
    <name>C5DN79_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0G14828p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0G14828g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555677.1">
        <property type="nucleotide sequence ID" value="XM_002555631.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DN79"/>
    <dbReference type="GeneID" id="8293962"/>
    <dbReference type="KEGG" id="lth:KLTH0G14828g"/>
    <dbReference type="eggNOG" id="ENOG502SCQR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_963355_0_0_1"/>
    <dbReference type="InParanoid" id="C5DN79"/>
    <dbReference type="OMA" id="DWHDIAN"/>
    <dbReference type="OrthoDB" id="4035321at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR25240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="289" mass="32237" checksum="87D4A279169ACFBB" modified="2009-07-28" version="1">MSNERDPACILHTNTTNNHPSKEVPSRRADTRTRTAHRMSSRFPSIHDAASYVNEELHRRALLPASQKLRFRSTDDKALQPQLASVNNDRLLINTIHKLLKALDRAEQVPETGPAEAAELPSPKPKPAPARASPPKTKVVKPTSRHTLASLRRYEVTIEQLRDKLRLDGPDLTWQVSPLPEPSAHVTSNKVTRDQDLDATTALLRKLIAHRDGSEKALAAVTEFLQASNSFLYTRCVHNCECSVPPPVAITQKQEKAEEPDLVAPIELAECLGKLQELVDDWHDIAQLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNA4</accession>
    <name>C5DNA4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Ferrochelatase</fullName>
            <ecNumber evidence="12">4.98.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0G15400g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the ferrous insertion into protoporphyrin IX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>heme b + 2 H(+) = protoporphyrin IX + Fe(2+)</text>
            <dbReference type="Rhea" id="RHEA:22584"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:57306"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <dbReference type="EC" id="4.98.1.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Porphyrin-containing compound metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the ferrochelatase family.</text>
    </comment>
    <dbReference type="EC" id="4.98.1.1" evidence="12"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555702.1">
        <property type="nucleotide sequence ID" value="XM_002555656.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNA4"/>
    <dbReference type="FunCoup" id="C5DNA4">
        <property type="interactions" value="754"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNA4"/>
    <dbReference type="GeneID" id="8293988"/>
    <dbReference type="KEGG" id="lth:KLTH0G15400g"/>
    <dbReference type="eggNOG" id="KOG1321">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018884_1_0_1"/>
    <dbReference type="InParanoid" id="C5DNA4"/>
    <dbReference type="OMA" id="DPYHCEC"/>
    <dbReference type="OrthoDB" id="1323at2759"/>
    <dbReference type="UniPathway" id="UPA00252">
        <property type="reaction ID" value="UER00325"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004325">
        <property type="term" value="F:ferrochelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006783">
        <property type="term" value="P:heme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00419">
        <property type="entry name" value="Ferrochelatase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03411">
        <property type="entry name" value="Ferrochelatase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1400:FF:000003">
        <property type="entry name" value="Ferrochelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1400">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00323">
        <property type="entry name" value="Ferrochelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001015">
        <property type="entry name" value="Ferrochelatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019772">
        <property type="entry name" value="Ferrochelatase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033644">
        <property type="entry name" value="Ferrochelatase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033659">
        <property type="entry name" value="Ferrochelatase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00109">
        <property type="entry name" value="hemH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11108">
        <property type="entry name" value="FERROCHELATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11108:SF1">
        <property type="entry name" value="FERROCHELATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00762">
        <property type="entry name" value="Ferrochelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53800">
        <property type="entry name" value="Chelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00534">
        <property type="entry name" value="FERROCHELATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="6 12" id="KW-0408">Iron</keyword>
    <keyword evidence="10 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 12" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11 12" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR25265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="378" mass="43175" checksum="38A580A2FC93592B" modified="2009-07-28" version="1">MFRAQLPAMRAFSTVGGLFQKSPTGIVFMNMGGPSTVPETRDFLYQLFSDNDLIPISRKYQPMIAKFISKFRTPKIEKQYKEIGGGSPIRKWSEYQAKRVCEILDETCPETAPHKPYVAFRYARPLTSETYERMLKDGVRKAIAFSQYPQFSYSTTGSSINELWRQIKKLDPERTINWSVIDRWPTNKGLVKAFADNITAKLQEYPEDIRKDVVLLFSAHSLPMDVINTGDAYPAEVGATVYRVMEQLKFCNPYRLVWQSQVGPKPWLGAQTADVTEWLAPSEKGLIYIPIAFTSDHIETLFEIDLEVIGESEYKDKLKRCDSLNGSETFIEGCADLVKEHLESGELYSKQLPLDFRLGRSSDTFEHPSQVFGDHSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNE8</accession>
    <name>C5DNE8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0G16368p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0G16368g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555746.1">
        <property type="nucleotide sequence ID" value="XM_002555700.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNE8"/>
    <dbReference type="FunCoup" id="C5DNE8">
        <property type="interactions" value="596"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNE8"/>
    <dbReference type="GeneID" id="8294032"/>
    <dbReference type="KEGG" id="lth:KLTH0G16368g"/>
    <dbReference type="eggNOG" id="KOG0776">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014015_1_0_1"/>
    <dbReference type="InParanoid" id="C5DNE8"/>
    <dbReference type="OMA" id="AFDYYLH"/>
    <dbReference type="OrthoDB" id="9927103at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990234">
        <property type="term" value="C:transferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004659">
        <property type="term" value="F:prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001:SF69">
        <property type="entry name" value="ALL TRANS-POLYPRENYL-DIPHOSPHATE SYNTHASE PDSS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001">
        <property type="entry name" value="GERANYLGERANYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00723">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR25309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="470" mass="51858" checksum="368135C9B343915C" modified="2009-07-28" version="1">MLLSRSASIGRVRILEYVRSKSSFATALETAARLVTPKVLLNNPVSLVSKEMNTLAKNIVALMGSGHPTLNRVTGYYFEAEGKKVRPMLVLLLSRALAEIPIEERNRITVDNTDVPEDPVYSKPPQSLLFQHPAKSISPLHILHGIKPLNPLTKGPEPLPEDFDRERGILPKQRRLAEIVEMIHTASLLHDDVIDHSDTRRGRASGNVAFTNKMAVLAGDFLLGRATVSISRLRNPEVVELISNSIANLVEGEFMQLKNTAVDSDLTTVANGTQKIPPPSPKLTNPTHDYRVPVERGAVFSQEIVIDTAFDYYLHKTYLKTASLISKSCRAAAILSGAQETVVDECYNFGKNLGICFQLVDDMLDFTISAKELGKPAGADLQLGIATAPVLYAWKEDHSLGPLIQRNFSLPGDVERTAEAVAKYEGVKKTRELAYEYRDRALANLRAALPESDARSALEFLTNSILTRRK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-02-08" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNG5</accession>
    <name>YFH7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName>ATP-dependent kinase YFH7</fullName>
            <ecNumber>2.7.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">YFH7</name>
        <name type="ordered locus">KLTH0G16808g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">ATP-dependent kinase that could be involved in endoplasmic reticulum membrane assembly.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the YFH7 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.-"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555763.1">
        <property type="nucleotide sequence ID" value="XM_002555717.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNG5"/>
    <dbReference type="SMR" id="C5DNG5"/>
    <dbReference type="FunCoup" id="C5DNG5">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNG5"/>
    <dbReference type="GeneID" id="8294052"/>
    <dbReference type="KEGG" id="lth:KLTH0G16808g"/>
    <dbReference type="eggNOG" id="KOG2702">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067202_1_0_1"/>
    <dbReference type="InParanoid" id="C5DNG5"/>
    <dbReference type="OMA" id="LYDQENW"/>
    <dbReference type="OrthoDB" id="6362633at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10285">
        <property type="entry name" value="URIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000404215" description="ATP-dependent kinase YFH7">
        <location>
            <begin position="1"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="33"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="347" mass="38972" checksum="FC0CF797063DBB44" modified="2009-07-28" version="1">MTFETAEGLADQVLKFLSDKLETNYRVAVIVVGPPGSGKSTISEKLCHEINSRYNKYLKESGSRPHLQENLNERIDLCEGIPKFEEKSLHDVQNGFFNHVQDQDFQPKKFVDKNDGSEVVVGIGGLPNSIRVENVAPLEPSNHDYKIAKIVPMDGFHLSRRHLDHFDDPVEAHRRRGSPPTFDSNNCLQLCKLLAKTCTIKPTLPVNKTTADTGTLFDKISDTFSESVPSIYVPGFDHALKDPSTGQHCVDAFTRIIVLEGLYLLLDEDNWRDIYPTFKDTHAVIVWKLDLGVDVLEQRVAKRHLQSGLAATLEAGVERFRMNDLINALRIKEHCLAADDIVSISNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNG7</accession>
    <name>C5DNG7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0G16852p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0G16852g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555765.1">
        <property type="nucleotide sequence ID" value="XM_002555719.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNG7"/>
    <dbReference type="FunCoup" id="C5DNG7">
        <property type="interactions" value="158"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNG7"/>
    <dbReference type="GeneID" id="8294054"/>
    <dbReference type="KEGG" id="lth:KLTH0G16852g"/>
    <dbReference type="eggNOG" id="ENOG502QWJ1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_399675_0_0_1"/>
    <dbReference type="InParanoid" id="C5DNG7"/>
    <dbReference type="OMA" id="YGVTICY"/>
    <dbReference type="OrthoDB" id="5427633at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008278">
        <property type="term" value="C:cohesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007062">
        <property type="term" value="P:sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21790">
        <property type="entry name" value="Rad21_Rec8_M_ScRec8p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039781">
        <property type="entry name" value="Rad21/Rec8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006910">
        <property type="entry name" value="Rad21_Rec8_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12585:SF51">
        <property type="entry name" value="MEIOTIC RECOMBINATION PROTEIN REC8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12585">
        <property type="entry name" value="SCC1 / RAD21 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04825">
        <property type="entry name" value="Rad21_Rec8_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad21/Rec8-like protein N-terminal" evidence="4">
        <location>
            <begin position="17"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="416"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="489"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="535"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="420"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="503"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR25328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="658" mass="74002" checksum="87C070A3108B82D6" modified="2009-07-28" version="1">MDLSNQQRLQLQRSNAHLDAYSGVTTVWLLSTLGGSRNTKAVGVNKRDIMSLSIPKTCEVLLQKKSDYTLRSASNLLYGVTVCYGRKTDYVLADIVSVKSQLQRQLLETVDRRGRILAQNATPTTIFDGVNEYRSLLEFQKFQSAFHQQRRSSAALLVDDPLFDINQTRDISFLLGKGHETNNSASSIHQRDIMDELKNGYSHETGAHAPFFEKQRLYADHEDLSALNADIHLDFDDVMSDVEMGSAKSSDGRNSERGDRAGFELSFDTGHIEPSENIEVPDLDIQLLGAADVDGNTKRVYPATDLEKDVIVKKKSKSAFSSGSLVRLKLDDRIGLPTETLRENNEHYCDVMDAQAHPRIKTHRKSTHEENWREVVFGQGQSPFLRFCFEEVLLKPALQNPQDTSSSSIDFIERGRQKMRSLPSSRSSSSAMSTERGRKQGPADLSKVGSGSGNNLFDLPELEQIDEDLEARYYSDDLDNDIMKIDLHLPPSSFGRSSSKTDGGTKDHIDELRHVHTERRLNRDGTLQSIEDYRDGSNQSEQHNKSKEKQIQSQALDNQSRRLFEYVLERASIVGKTTRTHPPFERKLLFEDLIPSNISRKHPDYSSEDGLSSVSRKICANAFLSLLQLASKANIDLHSFHSDTSLKTLGGDDIVIYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNM4</accession>
    <name>C5DNM4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0G18326p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0G18326g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555822.1">
        <property type="nucleotide sequence ID" value="XM_002555776.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNM4"/>
    <dbReference type="FunCoup" id="C5DNM4">
        <property type="interactions" value="454"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNM4"/>
    <dbReference type="GeneID" id="8294117"/>
    <dbReference type="KEGG" id="lth:KLTH0G18326g"/>
    <dbReference type="eggNOG" id="KOG2355">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057592_3_0_1"/>
    <dbReference type="InParanoid" id="C5DNM4"/>
    <dbReference type="OMA" id="YLGTEWV"/>
    <dbReference type="OrthoDB" id="6512918at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001930">
        <property type="entry name" value="ABC ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43158">
        <property type="entry name" value="SKFA PEPTIDE EXPORT ATP-BINDING PROTEIN SKFE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43158:SF2">
        <property type="entry name" value="SKFA PEPTIDE EXPORT ATP-BINDING PROTEIN SKFE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="5"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR25385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="269" mass="30745" checksum="E3916A6100F9585D" modified="2009-07-28" version="1">MSLAAEVRDLTYKFPHSDKASLCNIEIEIPWNTRTLIIGSNGAGKSTLLKLLSGKHLCLTGKIRVNGLDPFSPHSMHQNEADECQITTYLGTEWCHMSIIHRDIGVLELLESIGFHAHRERGETLIDILDVDVNWRMHRLSDGQKRRVQLTMGLLKPWRLLLLDEVTVDLDVRARSRLLSFLEQETKARRCSIVYATHIFDGLAEWPDRVVHLRDGRIVQQLSYAEEVRFSREDSSVVEHNDNSVTIGYAKSLHPLALTWLSQDETSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNT7</accession>
    <name>C5DNT7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0G19756p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0G19756g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555885.1">
        <property type="nucleotide sequence ID" value="XM_002555839.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNT7"/>
    <dbReference type="FunCoup" id="C5DNT7">
        <property type="interactions" value="42"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNT7"/>
    <dbReference type="GeneID" id="8294182"/>
    <dbReference type="KEGG" id="lth:KLTH0G19756g"/>
    <dbReference type="eggNOG" id="KOG1208">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082210_0_0_1"/>
    <dbReference type="InParanoid" id="C5DNT7"/>
    <dbReference type="OrthoDB" id="2898509at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052228">
        <property type="entry name" value="Sec_Metab_Biosynth_Oxidored"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47534:SF3">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE-LIKE C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47534">
        <property type="entry name" value="YALI0E05731P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR25448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="303" mass="33228" checksum="7996772102BEADC1" modified="2009-07-28" version="1">MGLGLKIDRAQENKELVWTNVPASQLKLKNLKVAIVGGTSGIGQSLARLFQSREAEVVVVGRTFRDHDLANIQFIKADLELMSEAKRVAKKLAQYSLDVVIFTAGILAAPTREETVEGLERDVAVSYLSRLVIIRNLLPALEKANSKFLTRPRVFIFGFPCQGEMGTPDDLNSTGKYNGMKTHMSTVAGNETLVFDTARRYPGVGVYGLNPGLIKTTIRDNFLGAGSFKSWVIEGIIGFLFQSSENYASKIAPMMVATELDNQSPAFFDSNGTAVLSKGFTNSYIEKYISKSEELLNECAIPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5E1W9</accession>
    <name>C5E1W9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0H00286p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0H00286g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555892.1">
        <property type="nucleotide sequence ID" value="XM_002555846.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E1W9"/>
    <dbReference type="GeneID" id="8294203"/>
    <dbReference type="KEGG" id="lth:KLTH0H00286g"/>
    <dbReference type="eggNOG" id="KOG3378">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_568657_0_0_1"/>
    <dbReference type="InParanoid" id="C5E1W9"/>
    <dbReference type="OrthoDB" id="4036345at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008941">
        <property type="term" value="F:nitric oxide dioxygenase NAD(P)H activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019825">
        <property type="term" value="F:oxygen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071500">
        <property type="term" value="P:cellular response to nitrosative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046210">
        <property type="term" value="P:nitric oxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01040">
        <property type="entry name" value="Mb-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.490.10">
        <property type="entry name" value="Globins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000971">
        <property type="entry name" value="Globin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009050">
        <property type="entry name" value="Globin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012292">
        <property type="entry name" value="Globin/Proto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044399">
        <property type="entry name" value="Mb-like_M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43396:SF6">
        <property type="entry name" value="ABL201WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43396">
        <property type="entry name" value="FLAVOHEMOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00042">
        <property type="entry name" value="Globin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46458">
        <property type="entry name" value="Globin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01033">
        <property type="entry name" value="GLOBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Globin" evidence="2">
        <location>
            <begin position="164"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="383"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="529"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="383"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="535"/>
            <end position="544"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS01033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR30030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="552" mass="59981" checksum="2F8BC1B93152936A" modified="2009-07-28" version="1">MFVGTNHRPNAAPQFVESLLTESQSTEDICLQSGDLKHRVSSDRSSERIRLLRQSAVSSDTYPRSFLDAYHDEFSSSSSNGELTSERGESEKGDEAKIPKGITPEASVTFQKHYKMSLQLQPHEIEMVRCSWSILLDDECSKPKYDAFVDKCVKPRINVPYKPPANEDSKNNVKEQLANSGKYEDDLKVPTFLFGAQFLHNIREIIPTIENSFPGIQHAASGVTGVVGMAISNLEDLSVMDYYLSSLGKHHARILGVYASHFDVAGIAFLKTLRDRFGYHYTKELDELWSRIYIYLANSMLQYGIDPILQDSGSSEITLGFPQLKEDSLSTSNADLSSKDSCGENEKIVGSLSDLSKNSTYSSKSAKEVSLVSSNKLLSRTSGWQSSKSSASSGQEGINSKTKKKEPTRNVGSVRGGGAWSGSHPQPTLAEESNVHYGASVRGGGGWQRSSGRSPSEKDFKGNSIGVVNDGKHALSTSRINGKSNFSLPASRPGATYAGKTSDITGKTHLRCSENYEMVTKAYSTVNSTARSSGGVRGGGGFNSGNGDCTLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>C5E242</accession>
    <name>C5E242_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">T-complex protein 1 subunit delta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0H01980g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555965.1">
        <property type="nucleotide sequence ID" value="XM_002555919.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E242"/>
    <dbReference type="FunCoup" id="C5E242">
        <property type="interactions" value="1538"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E242"/>
    <dbReference type="GeneID" id="8294278"/>
    <dbReference type="KEGG" id="lth:KLTH0H01980g"/>
    <dbReference type="eggNOG" id="KOG0358">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008891_9_1_1"/>
    <dbReference type="InParanoid" id="C5E242"/>
    <dbReference type="OMA" id="HPAANMI"/>
    <dbReference type="OrthoDB" id="10248520at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03338">
        <property type="entry name" value="TCP1_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000010">
        <property type="entry name" value="T-complex protein 1 subunit delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012717">
        <property type="entry name" value="Chap_CCT_delta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02342">
        <property type="entry name" value="chap_CCT_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR30103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="528" mass="57345" checksum="33856B7E06123D27" modified="2009-07-28" version="1">MTGKASTNSTFKNREKPQEVRKANIIAARAVADAIRTSLGPKGMDKMIKTSRGEVIISNDGHTILKQMAILHPVAKMLVEVSAAQDTEAGDGTTSVVILTGALLGAAEKLLNKGVHPTIIAESFQRAAKRSVEILLDMSQKISLSDKQALTRAAATSLSSKIVSQHSSFLAPLAVDCVLSIIDENATNVDLNDIRLIKKVGGTIDDTEMVDGVVLTQNVVKTAGGPTRIEKAKIGLIQFQISPPKPDTENNIVVNDYRQMDKILKEERAYLLNICKKIKKAKCNVLLIQKSILRDAVNDLALHFLSKLGIMVIKDVEREEIEFLSKSLNCKPISDPEFFTEDRLGAADLIEEVDSDGSKIVKITGVKSNVARPTVSVIVRGANNVILDETERSLHDALCVIRCLVKERALIAGGGAPEIEASRTLMKEARTLQGVEAFVWQEFAAALEVIPTTLAENAGLYSIRVVTDLRTLHENGEKNAGISIRRSATTNTFDEHVLQPVLVSTSAITLASECVKSILRIDDITFSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5E244</accession>
    <name>C5E244_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0H02024p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0H02024g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555967.1">
        <property type="nucleotide sequence ID" value="XM_002555921.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E244"/>
    <dbReference type="FunCoup" id="C5E244">
        <property type="interactions" value="139"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E244"/>
    <dbReference type="GeneID" id="8294280"/>
    <dbReference type="KEGG" id="lth:KLTH0H02024g"/>
    <dbReference type="eggNOG" id="KOG1536">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006150_1_1_1"/>
    <dbReference type="InParanoid" id="C5E244"/>
    <dbReference type="OMA" id="HHAFYSN"/>
    <dbReference type="OrthoDB" id="10250105at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004077">
        <property type="term" value="F:biotin--[biotin carboxyl-carrier protein] ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03144">
        <property type="entry name" value="GATase1_ScBLP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019197">
        <property type="entry name" value="Biotin-prot_ligase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003142">
        <property type="entry name" value="BPL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12835">
        <property type="entry name" value="BIOTIN PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12835:SF5">
        <property type="entry name" value="BIOTIN--PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02237">
        <property type="entry name" value="BPL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03099">
        <property type="entry name" value="BPL_LplA_LipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09825">
        <property type="entry name" value="BPL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="1">
        <location>
            <begin position="368"/>
            <end position="599"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR30105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="682" mass="74896" checksum="0750A7CA2117FD67" modified="2009-07-28" version="1">MNVLVYNGPGASPDSVKHALNTLRRLLEPFYAVCTTNARVLQTEPWAGKTSAVVFPGGADLPYTKECASVISQLKKFVSKQGGTFIGFCAGGYFATNCVEFAQGDPQLEVVGKRELKFFPDTGRGPAFHGFKYNSEAGARAASLRLSNGEELKTYYNGGCVFVNAEKYENVEVLAHYNEALDVPYSQDPSSLGAAVVLCQIGKGRALLTGPHPEFVPKLLQKTNDDKYNSSVVEVLLEYEKGRLNFMKDILTKAGLHCNNDFADRVAPSLTPLLVMASSNNHLIEELENNVRNSDGIFEDGAAILEGETDSFRIYKGFDQYEAAKSELLGEEPDEVPKSIIISSASEPFAPRKLTPNFDTAKYFQYLNPQSSVGSLLMYGEVITSTSSLLNNNKKLLSLFPRNSMLHVGTIQVSGRGRGGNTWVNPKGVSASTACINLPLVSPTTGRPVSIVFVQYLAMLAYCKAILSYAPGFEDLPVKIKWPNDLYAMKPEYYRTKKLRLTGRGLAGSKTPLTDVEPAYVKISGLLVNTNFLNNCYSLLLGCGLNVSHDGPTTSLNSWVSILNKEREKANMNPLPFVEIEKLQALYMNNLNVVLDTFLNKGPVSVLPEYYDLWLHNDQIVTLTDHQNVKAKIVGITKDYGLLIAKELVSGTNTQFTGNVYHLQPDGNTFDIFKGLISKKVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>C5E248</accession>
    <name>C5E248_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0H02112p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0H02112g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555971.1">
        <property type="nucleotide sequence ID" value="XM_002555925.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E248"/>
    <dbReference type="FunCoup" id="C5E248">
        <property type="interactions" value="41"/>
    </dbReference>
    <dbReference type="GeneID" id="8294284"/>
    <dbReference type="KEGG" id="lth:KLTH0H02112g"/>
    <dbReference type="eggNOG" id="KOG4138">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_169171_0_0_1"/>
    <dbReference type="InParanoid" id="C5E248"/>
    <dbReference type="OMA" id="CWKRQGN"/>
    <dbReference type="OrthoDB" id="5586401at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039870">
        <property type="entry name" value="Coa4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13639">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 4 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13639:SF2">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 4 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR30109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="82" mass="9832" checksum="F8C9E765E3195CC2" modified="2009-07-28" version="1">MAQKEDSKYYKEALEEYKELCNDEEDVWDKRINDTGCYVENMALQLCHADTNDWRQCLGEMALFRKCWDQKGNRERVSTVDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>C5E274</accession>
    <name>C5E274_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">KLTH0H02684p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0H02684g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555997.1">
        <property type="nucleotide sequence ID" value="XM_002555951.1"/>
    </dbReference>
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        <property type="entry name" value="XnlR_reg_dom"/>
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        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47424">
        <property type="entry name" value="REGULATORY PROTEIN GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47424:SF3">
        <property type="entry name" value="REGULATORY PROTEIN GAL4"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Fungal_trans"/>
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        <property type="entry name" value="Gal4_dimer"/>
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        <property type="entry name" value="Zn_clus"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
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        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
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        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
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        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0299">Galactose metabolism</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="12">
        <location>
            <begin position="10"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="613"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="656"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="627"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="657"/>
            <end position="668"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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            <dbReference type="ARBA" id="ARBA00023159"/>
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            <dbReference type="ARBA" id="ARBA00023163"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR30135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="749" mass="84719" checksum="1AFB1D8BB5371823" modified="2009-07-28" version="1">MFSLDEAEQACDWCRRKKLKCSREHPICSNCFKHNWDCHYSPKKVRSPLTRAHLTEVENRLHQLERLFGTLFPGYDWKDTSKDPSLSKLRHMLKNQKDQYERTLPITTDNLHAPSNVPSHPSQRPPLDQLPRDPLFGFEWSEEEDSQFLQNDGMGVLNVNINNKGYFGVGSNSVLLRALYVVDSLFTIQRVPSSSEDLSILSSKQIMDEYVEAYFGQFHAAFPIVDESYFRLCYAKKIIPTSEKAWQTLLYAVLALGSWSVVGEDSNIDIEYYKSAKYHLCSSVFETGSHSLLASLVLLSNYTQKRNKPNTSWNYLGLAASMAISLGLHREMRDSARTDNSGLEIRRRMWWVLYGYDCSMAMTFGRPGQLPKLPDVDILLPSNLDKNGVEVDSPTEFSFIIETAKLTKNAHKIISSSTGPYANLAETLKCHQKLDDLANKLPDYFSTGFARWHQKLGADNKWFPLNRFRLRWMYYNLTITIFRPLFLGILNGEKVRVDNHDFTKCLNLCHEAAGSTIESVSEFVSHYKLTTLSAWYATFFLVSATIIPAVGLVTEPGSSQAARWKLRVTTARNTFDVLKEKNATAEKFIHVIDSVCGYLLDTSEALSSTPKSFSSILHTEKRPGVAGTSSQQPTKDKSMTSISDLANILSPRDFTIPTDTVRSGSSETPDIAVQPENQDEDPFAWRSSSAQPLKQDATVFPNWNDQSVQTLFNPGTSNMFNTTTMDDIFNYVFNDNELAPSISNSSNQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2B2</accession>
    <name>C5E2B2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">endopeptidase La</fullName>
            <ecNumber evidence="7">3.4.21.53</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0H03542g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Hydrolysis of proteins in presence of ATP.</text>
            <dbReference type="EC" id="3.4.21.53"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the peptidase S16 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.53" evidence="7"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556035.1">
        <property type="nucleotide sequence ID" value="XM_002555989.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2B2"/>
    <dbReference type="STRING" id="559295.C5E2B2"/>
    <dbReference type="GeneID" id="8294348"/>
    <dbReference type="KEGG" id="lth:KLTH0H03542g"/>
    <dbReference type="eggNOG" id="KOG2004">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004109_4_0_1"/>
    <dbReference type="InParanoid" id="C5E2B2"/>
    <dbReference type="OMA" id="RCMNPVI"/>
    <dbReference type="OrthoDB" id="2411602at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19500">
        <property type="entry name" value="RecA-like_Lon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000021">
        <property type="entry name" value="Lon protease homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.5270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054594">
        <property type="entry name" value="Lon_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008269">
        <property type="entry name" value="Lon_proteolytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027065">
        <property type="entry name" value="Lon_Prtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008268">
        <property type="entry name" value="Peptidase_S16_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10046">
        <property type="entry name" value="ATP DEPENDENT LON PROTEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05362">
        <property type="entry name" value="Lon_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22667">
        <property type="entry name" value="Lon_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00830">
        <property type="entry name" value="ENDOLAPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51786">
        <property type="entry name" value="LON_PROTEOLYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01046">
        <property type="entry name" value="LON_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1 8" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Lon proteolytic" evidence="10">
        <location>
            <begin position="753"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="351"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="853"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="896"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00050665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00066743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR30173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="989" mass="110034" checksum="C704C04E43232711" modified="2009-07-28" version="1">MQFLSKRKEQTANYLELPCLLPQVTPSVVPLPGVRYRISIPKDKAAAMLQDLQEFVDISSYELSKGKELLRGPKKNKQTSKSEVRSSFQCIDEKLKVSNRSQIYICLLPTVQTNYTRGCVSILEEILAEGELVTFCLKGVRRAIIKDPEINLQNRLWISKLLVLDDGPYLTSLSPESCGKLVKQLQNNFTNITSAFDSFRATYRNAVRLQSGNSERFFLLSPLSNTLFILLSKGSFSKSWKLLFELYQSLEVHKPGNQKDAIFSLIDMTVAVLPVTNKQRLRCLELEDPLERAKELDATIHDLVALLASLRDGFKFFEAKVQDFSPGDKAAFVALQLSALRGFIEEPKKGSRFIRNPKSGPPSGARVGADKVEKENGDEDEDLKTIGAFIDNLEISEVHPDGLKMLKKDFRRLERLTPQSSEYNVLRGYFDTIMDIPFKAKDVSKSNIDLEECKKKLDNDHYGLLDVKKRLIEYLSVLKLNVDFKNEGSKAKPPIMLLVGPPGVGKTSIAKSIAGVLNRRFHRISLGGIHNEAEIRGHRRTYVGSMCGLIINALRKSGSMAPFILLDEIDKVLSVQAGGNRGAGLNGDPGAALLEVLDPEQNSTFTDHYVGFPVDLSNVLFFCTANNLTGISAPLLNRMEVIEIPGYTPKEKLNIGLKFLLPKQIQLNGLDKASIKVELTKEAWDDLVLEYTREPGVRGLERQIASIVRGKVVEYVQSHESEHKIVTSQELLKYIGFPLHPISRELVKKIKFADKCGVVNGLAYGSDGTGNVLVFEMVKMGKLQGNSSGPRIRTTGNLGKVLNESIEIATTLVKSLSERKIIHGLPSQAFNDFLNSEWHIHVPMGAVSKDGPSAGVAIALALISAALNKPVSPKLCLTGEITLRGKVLPIGGVREKLLGAQLFGMERVLLPLSNRPDVIEAVMKPEEYESCLASSSMPELRRVQQKWQLEAFYIDDFYDVMTKCWPREIQLTDARILISPSDNSIRALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2I5</accession>
    <name>C5E2I5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0H05258p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0H05258g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556108.1">
        <property type="nucleotide sequence ID" value="XM_002556062.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2I5"/>
    <dbReference type="FunCoup" id="C5E2I5">
        <property type="interactions" value="31"/>
    </dbReference>
    <dbReference type="GeneID" id="8294423"/>
    <dbReference type="KEGG" id="lth:KLTH0H05258g"/>
    <dbReference type="eggNOG" id="ENOG502S0PG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_408853_0_0_1"/>
    <dbReference type="InParanoid" id="C5E2I5"/>
    <dbReference type="OMA" id="HFRIESL"/>
    <dbReference type="OrthoDB" id="4061472at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="171"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR30246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="672" mass="72787" checksum="3A3A3BFA4E19DB39" modified="2009-07-28" version="1">MFSATWPRSCVSRGPCPLSLCHGLACVAPHGHALSGPAASLFPPRSQCSSGKTLYMTNSAKRGGSRAGYRCTAETHIVRAYRSTDDSLAILTKAKTLFFRARVCKQAPSCSATFHSGGTTSAGSGDESFAQYGSAFVTLDSVYKGGAQGRYRRRGAARRDTHTPKRACHKMNSSTPSTATQISLPRDSGCAPAPPSYTAAAGEAARQPRRLELSSLAARGTLLKHPEPTPPSFGYNAFDRGKNPCITSHDSSLLHVTLVPDRDCFYMAALEPQLLSRRDAADHAPAFEGRGVVSGTLRVVVKGPDALLLENVHVRLSGFCSEFARCASAGSATHSMRMVSATNCGTTPFVQDFIRFGTPASAGAANAASGPSAGLVLLPPGEYEYRFEFVVDAATFPASIETAYGSTIYRLEALVSMPKRKRRFGNKYESVFLTSAVTLKKALQHRYAALYQGFTAVTSLREEMLACDIFLSSRVVEFDAPLQVSLQMVKRDAQECQVLFTDVLVQQTLSIPCVDTATGVLEPTNFHQVQTVRLGNMDFCDEQQQCLNAAFPELRVPSTLKTVERNRSFFPHYEEPSLAARGLEPVRSRLRVAHELKIVIGVHINQPGDQQRPKSVRLTIKFPIALVDPDMGCCVNLPRYEPKSLIPLPSGLCKLDSDSAPSTPPEYSDAEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2K5</accession>
    <name>C5E2K5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0H05720p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0H05720g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556128.1">
        <property type="nucleotide sequence ID" value="XM_002556082.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2K5"/>
    <dbReference type="FunCoup" id="C5E2K5">
        <property type="interactions" value="631"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2K5"/>
    <dbReference type="GeneID" id="8294443"/>
    <dbReference type="KEGG" id="lth:KLTH0H05720g"/>
    <dbReference type="eggNOG" id="ENOG502QU1Y">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050082_0_0_1"/>
    <dbReference type="InParanoid" id="C5E2K5"/>
    <dbReference type="OMA" id="CWACRLK"/>
    <dbReference type="OrthoDB" id="416217at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050675">
        <property type="entry name" value="OAF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069:SF32">
        <property type="entry name" value="ARGININE METABOLISM REGULATION PROTEIN II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069">
        <property type="entry name" value="OLEATE-ACTIVATED TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="8">
        <location>
            <begin position="14"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="85"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="204"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="106"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="116"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="204"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR30266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="444" mass="51770" checksum="00D8DB69C218B421" modified="2009-07-28" version="1">MSNCNKTKARSFTGCWACRFKKRRCDENKPFCSLCLRHGDQCSYDIRLVWQNENIFSVNENKELVSWKKLRNLCKQPNRNRISRRRFQQMTQFRQISPPNSDDEDSDKKDAAHDDGESDEDRDEENDSSKKNTFTISVRRLKIYDNALDCVHGQTDKNFDQRFVDQQLGQLLTKLESSSQVAGDDRSGPFCVFKVDSFKKRNDSEKPVISRTSHETHNELSSLPNTPISETNSFLAMRLSTAEATSKFLSHHWPNLLLANDTNFCLQQPAYVKWLTTHIGSMGHLLHPDFLEELMNGSVDSSKWLSMIPHFSMECQALYLVLLVMAEREKDMVDFVSRWVLSQTQVSYLSFPLIAHSLKSCNKIAFLFHCHELLADATLQDLFQYELTSMLKINTVRLILQYWSNLMMDQICQCQDTTNSEMQLKFWEMQLQCNEEFYRDICLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2Z0</accession>
    <name>C5E2Z0_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chitin biosynthesis protein CHS5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0H08954g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CHS5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556263.1">
        <property type="nucleotide sequence ID" value="XM_002556217.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2Z0"/>
    <dbReference type="FunCoup" id="C5E2Z0">
        <property type="interactions" value="115"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2Z0"/>
    <dbReference type="GeneID" id="8294588"/>
    <dbReference type="KEGG" id="lth:KLTH0H08954g"/>
    <dbReference type="eggNOG" id="KOG1181">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019904_3_0_1"/>
    <dbReference type="InParanoid" id="C5E2Z0"/>
    <dbReference type="OMA" id="NGMSEVG"/>
    <dbReference type="OrthoDB" id="245697at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034044">
        <property type="term" value="C:exomer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000747">
        <property type="term" value="P:conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13945">
        <property type="entry name" value="Chs5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000077">
        <property type="entry name" value="Chitin biosynthesis protein CHS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.120.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031673">
        <property type="entry name" value="Chs5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052827">
        <property type="entry name" value="CHS_Export/Cell_Fusion_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031669">
        <property type="entry name" value="Fn3_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47351">
        <property type="entry name" value="CHITIN BIOSYNTHESIS PROTEIN CHS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47351:SF1">
        <property type="entry name" value="CHITIN BIOSYNTHESIS PROTEIN CHS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16892">
        <property type="entry name" value="CHS5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16893">
        <property type="entry name" value="fn3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="7">
        <location>
            <begin position="78"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="6">
        <location>
            <begin position="166"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="280"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="327"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="363"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="374"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="412"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="464"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="597"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="621"/>
            <end position="635"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00060872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00071189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR30401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="635" mass="68448" checksum="838B9DD37095C822" modified="2009-07-28" version="1">MASVEVSLTVGRLDASLALLTTKNHHVIEFPTMLLPDNIKAGSIVKIQVAEDADLERQELEQFQTLQDEILEKYGTHSPQSPVLKVVNVTQTSGVLGWEPIELGSAQLKSLLLYKDGVRSLMIPNPFKTTATKISGLSVDSEYTFQLKLCTTSGDLWSEKITLHTHKMTDMSGITACLGALDGIEGITREHIENSLKEIGAKPLQSHVTIDTTHFISNDKDSDDDEFHKAQQSNVPIVRPEWVRACELERRIVGVRGFYLDADPNNLASYKFLKLPPKEGQQPVSNAAEDGNVMATDSKSLKEPVEEGEDAVNGDISESATPIQAEKGSREDTVINDSAELKTEDEGTQASQVKLDEAVTNPEYDDGMPEADVAEPAAEFAAEPAAEPVAEPAAESVAESITETAAEPIAESTEEPAEEPAEEPAEEPAEEPAEEPAEEPADEPAEEPADEPEVPVKAEAEIESINNEDINTSRVNTELDESQDAQAVAENAGFHDQPPANTETTVTGEKDLQEAADENAGDFASRQEAEIQEASSTIEPNGMSEVGAVAPTSSQELRHGNAEEDEARAAGTQLDQVEVVGEDNSQLNQKEESTDVSAAEESAEPNASLPKPEANGTQTSSKKKKNKKNKKKGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2Z3</accession>
    <name>C5E2Z3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0H09020p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0H09020g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556266.1">
        <property type="nucleotide sequence ID" value="XM_002556220.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2Z3"/>
    <dbReference type="STRING" id="559295.C5E2Z3"/>
    <dbReference type="GeneID" id="8294591"/>
    <dbReference type="KEGG" id="lth:KLTH0H09020g"/>
    <dbReference type="eggNOG" id="KOG2241">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009710_6_4_1"/>
    <dbReference type="InParanoid" id="C5E2Z3"/>
    <dbReference type="OMA" id="KKCKLRG"/>
    <dbReference type="OrthoDB" id="19141at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004831">
        <property type="term" value="F:tyrosine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002547">
        <property type="entry name" value="tRNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051270">
        <property type="entry name" value="Tyrosine-tRNA_ligase_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11586">
        <property type="entry name" value="TRNA-AMINOACYLATION COFACTOR ARC1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11586:SF43">
        <property type="entry name" value="TYROSINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01588">
        <property type="entry name" value="tRNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50886">
        <property type="entry name" value="TRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 4" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="TRNA-binding" evidence="5">
        <location>
            <begin position="26"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR30404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="205" mass="22136" checksum="A015B694F085CB7E" modified="2009-07-28" version="1">MVRLTIALGSKCATRHYTVAARPQTGFSLLNLRIGFASQVGAHPNSDKMFVSQIKVSNGGADAFKQVCSGLRGFVTREDLEGSLLVLVDNMKKCKLRGEVSEAMILCGDDSVSGIVQPCKPANFDLTLVGQPVVLEGTPAGAQPTSRRIKNNEWLEVSSRLYVDNKARVVYKDPETQTETPLCVYNEEKAIPILVGSVSPGSPVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5E336</accession>
    <name>C5E336_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0H10010p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0H10010g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556309.1">
        <property type="nucleotide sequence ID" value="XM_002556263.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E336"/>
    <dbReference type="FunCoup" id="C5E336">
        <property type="interactions" value="305"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E336"/>
    <dbReference type="GeneID" id="8294636"/>
    <dbReference type="KEGG" id="lth:KLTH0H10010g"/>
    <dbReference type="eggNOG" id="KOG2722">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026460_2_0_1"/>
    <dbReference type="InParanoid" id="C5E336"/>
    <dbReference type="OMA" id="IFVVQTF"/>
    <dbReference type="OrthoDB" id="2499604at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004776">
        <property type="entry name" value="Mem_transp_PIN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31794">
        <property type="entry name" value="AUXIN EFFLUX TRANSPORTER FAMILY PROTEIN (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31794:SF2">
        <property type="entry name" value="AUXIN EFFLUX TRANSPORTER FAMILY PROTEIN (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03547">
        <property type="entry name" value="Mem_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="241"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="280"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="317"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="348"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="384"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR30447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="410" mass="45138" checksum="D16C5A1C26A3A8C2" modified="2009-07-28" version="1">MDDSSGLSFGHISYVTLESVLQVVIVAFSGFWCAYTGLLPKQGQKVISRLNVDVFTPCLIFSKLARSLSLAKILEIAVIPVFYAMTTGVSFMSGKLMSRILGLDKDESNFVVANSIFGNSNSLPVSLTVSLAYTLPGLLWDEIKDDNRDNVASRGILYLLIFQQIGHVLRWSYGYNTLMRWSGDRGHPSIASVSEQLEVPDSEAGRSETPSGISARSYSSLYKLKGKVMKMWEKIQAVMNPPLWAMVISVFVASVHPIQHEFFSKNGFINNTLSDAIKELGALSIPLILVVLGSNLYPSQDTPQMTRNHKKMVLGSIVGRMILPSCFMLPVIALAVKYIKVSILDDPIFLVCGFILTISPPAIQLTQITQLNEFFEAEMASVLFWGYVVLSLPISIFGVTAAMTVLKWAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3D5</accession>
    <name>C5E3D5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein DOM34 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0H12474g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the Dom34-Hbs1 complex, a complex that recognizes stalled ribosomes and triggers the No-Go Decay (NGD) pathway (PubMed:20890290). In the Dom34-Hbs1 complex, dom34 recognizes ribosomes stalled at the 3' end of an mRNA and engages stalled ribosomes by destabilizing mRNA in the mRNA channel. Following ribosome-binding, the Dom34-Hbs1 complex promotes the disassembly of stalled ribosomes, followed by degradation of damaged mRNAs as part of the NGD pathway.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the eukaryotic release factor 1 family. Pelota subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556408.1">
        <property type="nucleotide sequence ID" value="XM_002556362.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3D5"/>
    <dbReference type="FunCoup" id="C5E3D5">
        <property type="interactions" value="568"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3D5"/>
    <dbReference type="GeneID" id="8294747"/>
    <dbReference type="KEGG" id="lth:KLTH0H12474g"/>
    <dbReference type="eggNOG" id="KOG2869">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023334_3_1_1"/>
    <dbReference type="InParanoid" id="C5E3D5"/>
    <dbReference type="OMA" id="DDLWHLK"/>
    <dbReference type="OrthoDB" id="10249111at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990533">
        <property type="term" value="C:Dom34-Hbs1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070651">
        <property type="term" value="P:nonfunctional rRNA decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070966">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, no-go decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070481">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, non-stop decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032790">
        <property type="term" value="P:ribosome disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071025">
        <property type="term" value="P:RNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.60:FF:000004">
        <property type="entry name" value="Protein DOM34 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000008">
        <property type="entry name" value="Protein pelota homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.60">
        <property type="entry name" value="eRF1 domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.870">
        <property type="entry name" value="Pelota, domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042226">
        <property type="entry name" value="eFR1_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005141">
        <property type="entry name" value="eRF1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005142">
        <property type="entry name" value="eRF1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005140">
        <property type="entry name" value="eRF1_Pelota-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038069">
        <property type="entry name" value="Pelota/DOM34_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058547">
        <property type="entry name" value="Pelota_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004405">
        <property type="entry name" value="TF_pelota"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00111">
        <property type="entry name" value="pelota"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10853">
        <property type="entry name" value="PELOTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10853:SF0">
        <property type="entry name" value="PROTEIN PELOTA HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03464">
        <property type="entry name" value="eRF1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03465">
        <property type="entry name" value="eRF1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26356">
        <property type="entry name" value="Pelota_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01194">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159065">
        <property type="entry name" value="Dom34/Pelota N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0469">Meiosis</keyword>
    <keyword evidence="6 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0498">Mitosis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="eRF1/Pelota-like N-terminal" evidence="11">
        <location>
            <begin position="1"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR30546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="386" mass="43811" checksum="E57D19A4610CB8FE" modified="2009-07-28" version="1">MKLISQVTGNGNNPEQLTLLPQDKEDLFCLYNIINTDDEVIFKRMVTSKVDSTGKKKTTELIKLRLRIVSSEFEPQHEFLRYKGITTEDESGRANVDIPLGKYFSFTVDYKYSFTLLKYDYNNFVAKQLKEACNLESRSDIAAIVLQEGIAHICLLSSFSTILKNKIEYSLPKKKRGTDVAKFDEKVEKFYKATYESMKRHFDFAQLKVILLCSPGFYAKTLFDKILSYAQEEQNKTILANKSRFLVAHCSTGYLQGINEVLKNPAYGSRLQDAKNSKEALVMDEFLQHLNDDDFKAWYGEAEVTKASEMGAIDTLLVTDGLLRSDDLNMRKKCVGITQDVERAGGSVVVFSSLHSSGEELDRLTGLACILKFPIPDLDEFSEEDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3D6</accession>
    <name>C5E3D6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cell division control protein 10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0H12518g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Bud neck</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556409.1">
        <property type="nucleotide sequence ID" value="XM_002556363.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3D6"/>
    <dbReference type="FunCoup" id="C5E3D6">
        <property type="interactions" value="273"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3D6"/>
    <dbReference type="GeneID" id="8294749"/>
    <dbReference type="KEGG" id="lth:KLTH0H12518g"/>
    <dbReference type="eggNOG" id="KOG1547">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017718_7_1_1"/>
    <dbReference type="InParanoid" id="C5E3D6"/>
    <dbReference type="OMA" id="QCEFVYL"/>
    <dbReference type="OrthoDB" id="416553at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000144">
        <property type="term" value="C:cellular bud neck septin ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031105">
        <property type="term" value="C:septin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000921">
        <property type="term" value="P:septin ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043934">
        <property type="term" value="P:sporulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01850">
        <property type="entry name" value="CDC_Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000260">
        <property type="entry name" value="Cell division control 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016491">
        <property type="entry name" value="Septin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006698">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="4 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="8">
        <location>
            <begin position="34"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00069702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR30547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="331" mass="37783" checksum="419B4AB685B66202" modified="2009-07-28" version="1">MSIKTPPDNSLISPTGYVGFDTITSQIEHRLLKKGFQFNIMVVGHSGLGKSTLINTLFASHLIDSATGRDISKDPISKTTEIKVSSHSLVEDRVRLNINVIDTPGFGDQVNNDKVWEPMVKYIKEQHSQYLRRELTAQRERFIPDTRVHAVLYFIQPNGKGLTQLDIASLKRLTEIANVVPVIAKADTLTMDERAEFREIIQRQFEKFDFKIYPYQSEDLTEEELELNESIRSIIPFAVVGSEKEISVNGELVRGRKTRWGSIVVEDINQCEFVFLREFLIRTHLQDLIETTSLVHYEGFRSRQLIALKENASSRASATHMAQTSNTMLQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3D9</accession>
    <name>C5E3D9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0H12584p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0H12584g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WrbA family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556412.1">
        <property type="nucleotide sequence ID" value="XM_002556366.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3D9"/>
    <dbReference type="FunCoup" id="C5E3D9">
        <property type="interactions" value="227"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3D9"/>
    <dbReference type="GeneID" id="8294752"/>
    <dbReference type="KEGG" id="lth:KLTH0H12584g"/>
    <dbReference type="eggNOG" id="KOG3135">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051402_0_0_1"/>
    <dbReference type="InParanoid" id="C5E3D9"/>
    <dbReference type="OMA" id="HHGMLIM"/>
    <dbReference type="OrthoDB" id="504689at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032126">
        <property type="term" value="C:eisosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003955">
        <property type="term" value="F:NAD(P)H dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160020">
        <property type="term" value="P:positive regulation of ferroptosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.360:FF:000001">
        <property type="entry name" value="NAD(P)H dehydrogenase (Quinone) FQR1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010089">
        <property type="entry name" value="Flavoprotein_WrbA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005025">
        <property type="entry name" value="FMN_Rdtase-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01755">
        <property type="entry name" value="flav_wrbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002999">
        <property type="entry name" value="PRK03767.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30546">
        <property type="entry name" value="FLAVODOXIN-RELATED PROTEIN WRBA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30546:SF23">
        <property type="entry name" value="FLAVOPROTEIN-LIKE PROTEIN YCP4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03358">
        <property type="entry name" value="FMN_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="3">
        <location>
            <begin position="4"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="210"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="214"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR30550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="245" mass="26289" checksum="EE9E4EBD7EB8D1D8" modified="2009-07-28" version="1">MAKIAIITYSLYGHIDTLAKSIQKGVQAAGGQADLFRVEETLPEEVLEKMFAPAKPDIPIATTQVLEEYDAFLFGVPTRYGNVPAQWAAFWDQTGGLWVKGALQGKPAGLFVSTGSYGGGQEITIKTCMTYLVHHGLIYIPLGYKNVFAELSNVEEIHGGSAWGAGTLAGVDGSRQPSELELRMAVAQGKSFYEVAQHFPLSAKKAAKKEVGSAQKSSAAQRTTQQAPAKTEKKEPSKSGCCVMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3F5</accession>
    <name>C5E3F5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0H13002p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0H13002g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
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                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
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                <person name="de Montigny J."/>
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                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
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        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
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            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
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            <title>A HORMA domain in Atg13 mediates PI 3-kinase recruitment in autophagy.</title>
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                <person name="Ragusa M.J."/>
                <person name="Stanley R.E."/>
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        <citation type="journal article" date="2014" name="Nat. Struct. Mol. Biol." volume="21" first="513" last="521">
            <title>Structural basis of starvation-induced assembly of the autophagy initiation complex.</title>
            <authorList>
                <person name="Fujioka Y."/>
                <person name="Suzuki S.W."/>
                <person name="Yamamoto H."/>
                <person name="Kondo-Kakuta C."/>
                <person name="Kimura Y."/>
                <person name="Hirano H."/>
                <person name="Akada R."/>
                <person name="Inagaki F."/>
                <person name="Ohsumi Y."/>
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            <dbReference type="DOI" id="10.1038/nsmb.2822"/>
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        <scope>X-RAY CRYSTALLOGRAPHY (3.20 ANGSTROMS) OF 392-404</scope>
    </reference>
    <reference evidence="12" key="4">
        <citation type="journal article" date="2016" name="Dev. Cell" volume="38" first="86" last="99">
            <title>The Intrinsically Disordered Protein Atg13 Mediates Supramolecular Assembly of Autophagy Initiation Complexes.</title>
            <authorList>
                <person name="Yamamoto H."/>
                <person name="Fujioka Y."/>
                <person name="Suzuki S.W."/>
                <person name="Noshiro D."/>
                <person name="Suzuki H."/>
                <person name="Kondo-Kakuta C."/>
                <person name="Kimura Y."/>
                <person name="Hirano H."/>
                <person name="Ando T."/>
                <person name="Noda N.N."/>
                <person name="Ohsumi Y."/>
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            <dbReference type="DOI" id="10.1016/j.devcel.2016.06.015"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (3.21 ANGSTROMS) OF 392-404 AND 325-337</scope>
    </reference>
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        <text evidence="2 5">Belongs to the ATG13 family. Fungi subfamily.</text>
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            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the palH/RIM21 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551523.1">
        <property type="nucleotide sequence ID" value="XM_002551477.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBC4"/>
    <dbReference type="FunCoup" id="C5DBC4">
        <property type="interactions" value="63"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBC4"/>
    <dbReference type="GeneID" id="8290315"/>
    <dbReference type="KEGG" id="lth:KLTH0A01386g"/>
    <dbReference type="eggNOG" id="ENOG502QWMT">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026111_0_0_1"/>
    <dbReference type="InParanoid" id="C5DBC4"/>
    <dbReference type="OMA" id="CVVIPWE"/>
    <dbReference type="OrthoDB" id="5393256at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071467">
        <property type="term" value="P:cellular response to pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014844">
        <property type="entry name" value="PalH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35779">
        <property type="entry name" value="PH-RESPONSE REGULATOR PROTEIN PALH/RIM21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35779:SF1">
        <property type="entry name" value="PH-RESPONSE REGULATOR PROTEIN PALH_RIM21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08733">
        <property type="entry name" value="PalH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="92"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="176"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="210"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="254"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="287"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="382"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="500"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="411"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR21081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="521" mass="59814" checksum="8940D88B9835BA22" modified="2009-07-28" version="1">MVKKTSWRYSQVPKEYQSCGGIDLGNGVLIWKNKWQPVAYFDSVRFTSYCDEGNPVYSSLKRNRLSFPYSKALQRDWHTFITSGNDRGPFKYSIYAIIMSFTTNFVITLFLTVIVFITIRQKPHRGASNLLKLGSTLASVNLAIFISKALKVLHQDHIYKGVVSTERVLDLLWMDLTFTCLDLLVVLICQLCQVQIIMRLFSRVKEKRTVFLVGVTLSIISQVLWAIPTLAQSINDNFSGLSYDADGLTLLSPFVYLFRIALAASYACIICFNMFNKRHLCFLYQRMMILTLLALIIVLLQPGFFVADVANIWVDDLSEIFNTTCYVGSAVIVWEWVDHILILERKVQAQSVLGRPVYEDDQEDYHFAKYALKVQDAISRDSETGEIGSRNDTANSGTEEQRVRWQYGDDTPPRDTNKVEFNEPQPLKNVILEKCSRIWDGMIYYTDHFIVKGLVIKTLSIHSKSSSDDHRKRKAKVRRRIGLDRPNEVYVYATRDVVFDSDEDEAESNQGSSVDEERDFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBF3</accession>
    <name>C5DBF3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">60S ribosomal subunit assembly/export protein LOC1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">60S ribosomal subunit assembly/export protein loc1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0A02134g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LOC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551552.1">
        <property type="nucleotide sequence ID" value="XM_002551506.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBF3"/>
    <dbReference type="FunCoup" id="C5DBF3">
        <property type="interactions" value="418"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBF3"/>
    <dbReference type="GeneID" id="8290348"/>
    <dbReference type="KEGG" id="lth:KLTH0A02134g"/>
    <dbReference type="eggNOG" id="ENOG502RY6R">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096593_1_0_1"/>
    <dbReference type="InParanoid" id="C5DBF3"/>
    <dbReference type="OMA" id="RESMNTI"/>
    <dbReference type="OrthoDB" id="1743802at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008298">
        <property type="term" value="P:intracellular mRNA localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037650">
        <property type="entry name" value="Loc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28028">
        <property type="entry name" value="60S RIBOSOMAL SUBUNIT ASSEMBLY/EXPORT PROTEIN LOC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28028:SF1">
        <property type="entry name" value="60S RIBOSOMAL SUBUNIT ASSEMBLY_EXPORT PROTEIN LOC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="150"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="27"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="150"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="170"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR21110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="209" mass="23764" checksum="B734AFA5673213C3" modified="2009-07-28" version="1">MAGKQTKTAKRSNKQNGKREVNPEVFQDSQARNQLSSAPKLTEKSASRKPSKLKVSKEQALARLYGSKNKKVKAYSEKELNIPSLNRAVIPGVKFKRGKKGKKFVGDHDELLYSRLISTIGDKQEQLTESKLEKARRLEEIRELKRQEIERKEDAKKDKLDSKKDELKKKASVARTLRRKNKRDQYNESKAPAAASNDVGPKKKTVSFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBF8</accession>
    <name>C5DBF8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0A02266p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0A02266g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551557.1">
        <property type="nucleotide sequence ID" value="XM_002551511.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBF8"/>
    <dbReference type="FunCoup" id="C5DBF8">
        <property type="interactions" value="32"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBF8"/>
    <dbReference type="GeneID" id="8290354"/>
    <dbReference type="KEGG" id="lth:KLTH0A02266g"/>
    <dbReference type="eggNOG" id="KOG2016">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019618_2_0_1"/>
    <dbReference type="InParanoid" id="C5DBF8"/>
    <dbReference type="OMA" id="KLITHQY"/>
    <dbReference type="OrthoDB" id="1708823at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019781">
        <property type="term" value="F:NEDD8 activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045116">
        <property type="term" value="P:protein neddylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF29">
        <property type="entry name" value="NEDD8-ACTIVATING ENZYME E1 REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR21115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="447" mass="51138" checksum="8355ECD30E3CEFA5" modified="2009-07-28" version="1">MDKFDRQLRLWGKHGQLELSKAHICIIGNTLLASEILKNLVLPGATKFHLIKETSYVKSDCFLDVEDLKSINEHAEFNEETWNAHLMGNQEYWRYFDLIILTTRDVSVIESIRCLPMPPMLACNWREYHGYACFVSQEPHFVTECHRQHKSPDLRIDKPPQPLLNFYESIKPSQLSQEQLNDLPFAVIIYHALKSLKENCTKIDRSSLRIEIRRLHEKTLPGPECLNFIEAERYLYLGLQDSEIIPDNLLKCFSLASSECVVPFNSMFTDMISSLKDYLKHPSSEHQLPLSGLIPDMESSSEMYNAIQEAYLRCASKNLNTFADIVTSHNASVPLSQIKNFCSNVQNVRGVVPASHIPTEQDCRTVKQITPAAAAILRKLVKSKHELETIRPSFVESRDELRYYPTCSLLAGIVAQEAVKILTHQFVPLENTLIYDGKAGQVYRVKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBH0</accession>
    <name>C5DBH0_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribosome quality control complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0A02530g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NEMF family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551569.1">
        <property type="nucleotide sequence ID" value="XM_002551523.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBH0"/>
    <dbReference type="FunCoup" id="C5DBH0">
        <property type="interactions" value="1069"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBH0"/>
    <dbReference type="GeneID" id="8290366"/>
    <dbReference type="KEGG" id="lth:KLTH0A02530g"/>
    <dbReference type="eggNOG" id="KOG2030">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003612_1_1_1"/>
    <dbReference type="InParanoid" id="C5DBH0"/>
    <dbReference type="OMA" id="MFLEFFA"/>
    <dbReference type="OrthoDB" id="207084at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990112">
        <property type="term" value="C:RQC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990116">
        <property type="term" value="P:ribosome-associated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.310.10:FF:000003">
        <property type="entry name" value="Zinc knuckle domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.310.10">
        <property type="entry name" value="ibrinogen binding protein from staphylococcus aureus domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021846">
        <property type="entry name" value="NFACT-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008532">
        <property type="entry name" value="NFACT_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051608">
        <property type="entry name" value="RQC_Subunit_NEMF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15239">
        <property type="entry name" value="NUCLEAR EXPORT MEDIATOR FACTOR NEMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15239:SF6">
        <property type="entry name" value="RIBOSOME QUALITY CONTROL COMPLEX SUBUNIT NEMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11923">
        <property type="entry name" value="NFACT-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05670">
        <property type="entry name" value="NFACT-R_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05833">
        <property type="entry name" value="NFACT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NFACT RNA-binding" evidence="8">
        <location>
            <begin position="576"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="domain" description="NFACT protein C-terminal" evidence="9">
        <location>
            <begin position="932"/>
            <end position="1033"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="437"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="708"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1037"/>
            <end position="1058"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="529"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="441"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="462"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="473"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="489"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="710"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="747"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="825"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="851"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="887"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="908"/>
            <end position="917"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00070414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF05670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF11923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR21127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1058" mass="120638" checksum="C95B3A6293EB1D38" modified="2009-07-28" version="1">MKQRISSLDLELLYRELKSQLEGYRLSNIYNIAESSRQFLLKFNKPDSKLNAIIDCGLRVHLTDFTRPVPATPSGFVVKLRKHLKSKRLTTVKRVANDRILVLSFNDGQFFLVLEFFSAGNVILLDSDRKIIVLQRIVHEHENKVGHIYNMFDGSFLENTRIEPPKSKVHSADEVNGWIKEAKDFADSSVKAKTGKGAKVLSIHKLLFLREPQLSSDLISRNLKSRGIAPNSPCLNFLDKIDEIVDLLDATESEVNELLRDGCKLGYIIAKKNPHYDSEKGDANLEFVYEQFHPFPPHLSEDEKGYTKIIEVPGQYNKTVDDFFSTIESSKYALRIQNQEFQAKNRLESAKLDNEKRIQALIDVQTQNEVRGHAIIAAADLVEEAQNAIKALVEQQMDWKTIEVLISNEQKKNNRIARLIKLPLDLKNNKFTLSLPRNDEIESDNSDEEEDNLTSSEDETSSSDSSDSSLSDFEADDNDEDELTSVSNIKKDRNDNKKKEKPSIDATIDLTLSAYANASNYFNIKKSNVEKQKKVEKNAQKALKNIEQRIEKDLKKKLKESHDVLNKTRKPYFFEKFHWFVSSEGFLVLMGKSGMESDQIYGKYIHDNDVFVSNSFDTHVWIKNPDETEVPPNTLMQAGIMCMSASPAWSKKIQSSAWWCFAKELSKFDNYGGEVLPAGTFRLKDEKKKSFLPPSQLVMGFALLWKTKDSDEEEEEDGADDEDQDDEEAQKEKSVLDEDAVPGQVQGKDDELEISKDENRRIDEGFVIGKKMEGVKEHQSPTKSTDLKSVKEKSDSDEVPVTTGLYPKISNSDSDVQADKEPSEQSETNNNSENGSAFSDKSGSNKETIKKNVRGKKGKMKKMQKKYADQDEEERLMRLAVLGTLKGSERQQQKAEEEAAKLKEREQKKFRRERQKKQQSLMFGSEQKVNVNYAKIFSEISPVVDDKSKVRDIVPVYAPWPALTKYKYKVKVQPGNAKKTKSLNEILTYFSKRKVDETESDKELDWPCEHELIKKLKELDLVSILYTDKLKITIPGQNEVKSKTKGTPSSKGKKKGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBI3</accession>
    <name>C5DBI3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0A02860p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0A02860g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551582.1">
        <property type="nucleotide sequence ID" value="XM_002551536.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBI3"/>
    <dbReference type="FunCoup" id="C5DBI3">
        <property type="interactions" value="152"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBI3"/>
    <dbReference type="GeneID" id="8290381"/>
    <dbReference type="KEGG" id="lth:KLTH0A02860g"/>
    <dbReference type="eggNOG" id="ENOG502S44P">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102617_1_1_1"/>
    <dbReference type="InParanoid" id="C5DBI3"/>
    <dbReference type="OMA" id="NKGVFQG"/>
    <dbReference type="OrthoDB" id="2348401at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007250">
        <property type="entry name" value="HSP9_HSP12"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04119">
        <property type="entry name" value="HSP9_HSP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002590">
        <property type="entry name" value="HSP9/HSP12_fun"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="104" mass="11194" checksum="3CC095770C29FEF6" modified="2009-07-28" version="1">MSDSYRKDFSDKAAEKVKPDSQKSYAEQGKEFVTDQADKVAGKMQPEQEKGAFQGVNDAAHKGKSEATGKSMTEQAGEYVDAAKAKLNDAAEYVSSSLHGGEKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBI9</accession>
    <name>C5DBI9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Dihydrolipoyl dehydrogenase</fullName>
            <ecNumber evidence="11">1.8.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0A02992g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>N(6)-[(R)-dihydrolipoyl]-L-lysyl-[protein] + NAD(+) = N(6)-[(R)-lipoyl]-L-lysyl-[protein] + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15045"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="EC" id="1.8.1.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9 11">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="9 11">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="11">The active site is a redox-active disulfide bond.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.4" evidence="11"/>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551588.1">
        <property type="nucleotide sequence ID" value="XM_002551542.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBI9"/>
    <dbReference type="FunCoup" id="C5DBI9">
        <property type="interactions" value="1194"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBI9"/>
    <dbReference type="GeneID" id="8290387"/>
    <dbReference type="KEGG" id="lth:KLTH0A02992g"/>
    <dbReference type="eggNOG" id="KOG1335">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016755_0_1_1"/>
    <dbReference type="InParanoid" id="C5DBI9"/>
    <dbReference type="OMA" id="CAQLGMK"/>
    <dbReference type="OrthoDB" id="361797at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045252">
        <property type="term" value="C:oxoglutarate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004148">
        <property type="term" value="F:dihydrolipoyl dehydrogenase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006103">
        <property type="term" value="P:2-oxoglutarate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.30:FF:000001">
        <property type="entry name" value="Dihydrolipoyl dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000001">
        <property type="entry name" value="Dihydrolipoyl dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050151">
        <property type="entry name" value="Class-I_Pyr_Nuc-Dis_Oxidored"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006258">
        <property type="entry name" value="Lipoamide_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001100">
        <property type="entry name" value="Pyr_nuc-diS_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012999">
        <property type="entry name" value="Pyr_OxRdtase_I_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01350">
        <property type="entry name" value="lipoamide_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22912:SF151">
        <property type="entry name" value="DIHYDROLIPOYL DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22912">
        <property type="entry name" value="DISULFIDE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000350">
        <property type="entry name" value="Mercury_reductase_MerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="FAD/NAD-linked reductases, dimerisation (C-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00076">
        <property type="entry name" value="PYRIDINE_REDOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 9 11" id="KW-0274">FAD</keyword>
    <keyword evidence="2 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5 9 11" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7 11" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="13">
        <location>
            <begin position="29"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="Pyridine nucleotide-disulphide oxidoreductase dimerisation" evidence="12">
        <location>
            <begin position="379"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="477"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="175"/>
            <end position="177"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="212"/>
            <end position="219"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="345"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="351"/>
            <end position="354"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="10">
        <location>
            <begin position="66"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR21146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="498" mass="53666" checksum="D6C2DC017359E0FC" modified="2009-07-28" version="1">MLRMRLQAGASRRFLSGTSRMLAEKKKHDLVVVGSGPGGYVAAIKAAQLGFDTACVEKRGRAGGTCLNVGCIPSKALLNNSHLYHQMKHEAKQRGIDISGDVSVNVAQLQKAKDTSVKQLTGGIEMLFKKNGVTYYKGHGSFEDENNIKVSPVEGVEGSVTEETILEAKNIIVATGSEVTPFPGIKIDEERIVSSTGALSLKEVPKRLAIIGGGIIGLEMGSVYSRLGSKVSVVEFLPKIGATMDDEVASATQKFLKKQGFDFKLGTKVLSAERNGDVVNIEVENVKSGKKESLEADVLLVAIGRRPYIQGLNAEKLGLEVDKRGRLVIDEQFNTKFPHIKVIGDVTFGPMLAHKAEEEGIAAVEYLKEGHGHVNYANIPSVMYTHPEVAWVGKTEEQLKEAGISYKIGKFPFMANSRAKTNQDSDGFVKILIDAETERILGAHIIGPNAGEMIAEAGLALEYGASAEDVARVCHAHPTLSEAFKEANLAAYSKSINF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBT0</accession>
    <name>C5DBT0_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Dihydroorotate dehydrogenase (quinone), mitochondrial</fullName>
            <ecNumber evidence="5">1.3.5.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Dihydroorotate oxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0A05104g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(S)-dihydroorotate + a quinone = orotate + a quinol</text>
            <dbReference type="Rhea" id="RHEA:30187"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
            <dbReference type="ChEBI" id="CHEBI:30864"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.3.5.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.5.2" evidence="5"/>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551679.1">
        <property type="nucleotide sequence ID" value="XM_002551633.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBT0"/>
    <dbReference type="STRING" id="559295.C5DBT0"/>
    <dbReference type="GeneID" id="8290483"/>
    <dbReference type="KEGG" id="lth:KLTH0A05104g"/>
    <dbReference type="eggNOG" id="KOG1436">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013640_4_0_1"/>
    <dbReference type="InParanoid" id="C5DBT0"/>
    <dbReference type="OMA" id="IYGTDTR"/>
    <dbReference type="OrthoDB" id="14784at2759"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00946"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106430">
        <property type="term" value="F:dihydroorotate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04738">
        <property type="entry name" value="DHOD_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050074">
        <property type="entry name" value="DHO_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005719">
        <property type="entry name" value="Dihydroorotate_DH_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005720">
        <property type="entry name" value="Dihydroorotate_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001295">
        <property type="entry name" value="Dihydroorotate_DH_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003645">
        <property type="entry name" value="PRK05286.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003652">
        <property type="entry name" value="PRK05286.2-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01036">
        <property type="entry name" value="pyrD_sub2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48109:SF4">
        <property type="entry name" value="DIHYDROOROTATE DEHYDROGENASE (QUINONE), MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48109">
        <property type="entry name" value="DIHYDROOROTATE DEHYDROGENASE (QUINONE), MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01180">
        <property type="entry name" value="DHO_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00912">
        <property type="entry name" value="DHODEHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0288">FMN</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dihydroorotate dehydrogenase catalytic" evidence="13">
        <location>
            <begin position="102"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR21237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="439" mass="47817" checksum="C3219FCB577619DB" modified="2009-07-28" version="1">MFVRPTLRGLSKTPNYFLKNSKLPLKSGFNGGKFILYTAGAIGGGFAGLYLLNSRSAIHEYLFCPLIRLVTPDAETGHQLGVFFLKWGLAPKLLFDKDDEVLKVNVFGTILTNPVGCAAGLDKDGEAIDGILQSGFSYHEIGSITPLPQPGNPRSRFFRLPQDEAVINRYGFNSTGKDSVQGTLVERASKYVKSYFKDADVGKMSLYKNKLLAINLGKNKNGDEVQDYLKGVETFQSLADVLVINVSSPNTPGLRDLQNESKLADLLQKVVDKRNSLVDAGNQLGAKAHKPPVLVKIAPDLTEPELLSIAEAAKKSKVDGIIVSNTTIQRPNSLITQDPEVKFQTGGLSGKPLKQYSLKALRTIYKYTKDSDLVLIGCGGISSAQDALEYARAGATFVQLYTSFAYKGPGVVSKIKDGLTEELKREGKTWMQIIGEDSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5DC25</accession>
    <name>C5DC25_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0A07260p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0A07260g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551774.1">
        <property type="nucleotide sequence ID" value="XM_002551728.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DC25"/>
    <dbReference type="FunCoup" id="C5DC25">
        <property type="interactions" value="83"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DC25"/>
    <dbReference type="GeneID" id="8290581"/>
    <dbReference type="KEGG" id="lth:KLTH0A07260g"/>
    <dbReference type="eggNOG" id="ENOG502SJDI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010594_0_0_1"/>
    <dbReference type="InParanoid" id="C5DC25"/>
    <dbReference type="OMA" id="YLENLWH"/>
    <dbReference type="OrthoDB" id="4356994at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052693">
        <property type="entry name" value="Yeast_MDR_Regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31405">
        <property type="entry name" value="TRANSCRIPTION FACTOR PDR8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31405:SF8">
        <property type="entry name" value="TRANSCRIPTION FACTOR PDR8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="2">
        <location>
            <begin position="35"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="662"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR21332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="733" mass="84575" checksum="0B688A54941C9DF5" modified="2009-07-28" version="1">MSSEDHVEDEGGFEPNEDQFEVDGRSKKRRKAIKQCLFCRKRKLKCDKKKPMCTTCVTRGLGECIYVEQFGRDVSARELLNSAPNVDLLNKIKALEKELDSYKHTSLTKGEGFNPLRDVQILIKKDERTIYHGCTSFRAALSESRFKFSTYHAFFWARVKDDRRRWKALNGYTTLKEITVIEKERDNGLSVLGAVCKALPPFEQIEAIVKKFFTSILLEGYKVLDMKKTLQNLHMSFIRGPQNPITGMHPVIHLWPSFKKNYYCIGIILEIMCLMSYSSVIPEAIDVFNKYLTSFVTKKAFYIERVQYLFLRYLYKSLNYGGGDDSNLVFLAQAMSASAICVGLHRNIRDLLKGDNVDRGDAVYFENLWLWILQADLETSFNVGTPPKIAESFVNYQMIEDPNYGSCELLKNLIRPMRNIMTVIYLENRIPDMEAIVGKIKRLLETHFKPLKLYREKENLKQIPFVEIDLYTWLLGMIGVFSNFRRVYFKDHSPSAINSALQFTILPLAMSMSTIEAFFELDKEAAAKTQAPLQGGLPFHLAIANSLVQKHFPRSLSEIYTLMGSLGSFPDFGTARFQYPLSPVFDIPLDSLECVGDHYVSFKQASEVIENLLSKWEEEKNRDMVKLLKRYSYAFVVSYAIQKSHKDLFDQCFKRRELQFTRGEEQSNPDMEQKAALPSPSNSFGGQEFIPDETLKELADEFWKNYDIEVDDFFSDAFDQTSIWFDSSAQPQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCH2</accession>
    <name>C5DCH2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">dynamin GTPase</fullName>
            <ecNumber evidence="2">3.6.5.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0B03058g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.5"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.5" evidence="2"/>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551921.1">
        <property type="nucleotide sequence ID" value="XM_002551875.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCH2"/>
    <dbReference type="FunCoup" id="C5DCH2">
        <property type="interactions" value="1422"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCH2"/>
    <dbReference type="GeneID" id="8290754"/>
    <dbReference type="KEGG" id="lth:KLTH0B03058g"/>
    <dbReference type="eggNOG" id="KOG0446">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008964_5_0_1"/>
    <dbReference type="InParanoid" id="C5DCH2"/>
    <dbReference type="OMA" id="KICHNCG"/>
    <dbReference type="OrthoDB" id="5061070at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048312">
        <property type="term" value="P:intracellular distribution of mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030001">
        <property type="term" value="P:metal ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000266">
        <property type="term" value="P:mitochondrial fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016559">
        <property type="term" value="P:peroxisome fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08771">
        <property type="entry name" value="DLP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1240:FF:000002">
        <property type="entry name" value="Dynamin-1-like protein isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1240">
        <property type="entry name" value="Dynamin, middle domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022812">
        <property type="entry name" value="Dynamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001401">
        <property type="entry name" value="Dynamin_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019762">
        <property type="entry name" value="Dynamin_GTPase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000375">
        <property type="entry name" value="Dynamin_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003130">
        <property type="entry name" value="GED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020850">
        <property type="entry name" value="GED_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566">
        <property type="entry name" value="DYNAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566:SF235">
        <property type="entry name" value="DYNAMIN-RELATED PROTEIN DNM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01031">
        <property type="entry name" value="Dynamin_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02212">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00195">
        <property type="entry name" value="DYNAMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00053">
        <property type="entry name" value="DYNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00302">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00410">
        <property type="entry name" value="G_DYNAMIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51718">
        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51388">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynamin-type G" evidence="14">
        <location>
            <begin position="25"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="GED" evidence="13">
        <location>
            <begin position="684"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="579"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="630"/>
            <end position="675"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR21483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="771" mass="86513" checksum="331F846B94F173F5" modified="2009-07-28" version="1">MASLEDLIPTVNKLQDVMYDAGIDTLDLPVLAVIGSQSSGKSSILETLVGKDFLPRGTGIVTRRPLVLQLNNISPNSPLINEYQDIHGQNYDTSDTKGELTLEDHLRKSQGFEPTTKDEWGEFLHLPGRRFYDFGEIRQEIENETARIAGKNKGISRIPINLKVFSPRVLNLTLVDLPGITKVPIGEQPADIEKQIKNLILEHVAKPNCIILAISPANVDLVNSESLKLARDVDPHGKRTIGVITKMDLMDSGTNALDILSGKLYPLKLGFVGIVNRSQQDIQANRSVEEALNNEEIFFTRHPVYRTISTKCGTRYLAKLLNHILMNHIRDKLPDIKARLNTLMGQTEQELATYGGSGIITKENRAGLVLQLMNKFAARFVSSIEGTSSDISTKELCGGARIYYIYNNIFGHSLESINPTSNLSTADIRTAIRNSTGPRPSLFVPELAFDLLVKPQIYLLLEPSQRCVEMVYEELMKICHNCGSPELARYPKLQAKLIEVVSELLRERLGPTRSYVESLIDIHRAYINTNHPNFLSATDAMSDIAEAKKNKQKATPGKSDLENAKISLVKELSRKDAELQFSNATGKEIPESSTPSEASSEEEERIPSKESKDSFLNYFFGKDQQKSGHFTKFPENGQFPGSHSFDQAENAPPSSDFEKLQINGSTTERTFDEPKLTEREELECELIRRLIISYFGIVREMIQDQIPKAVMCLLVNFSKEIIQNRLVTKLYKESLFEELLLEDQNLAQDREKCVKLLDTYKEASKIIGDIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCJ3</accession>
    <name>C5DCJ3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pumilio homology domain family member 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0B03520g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PUF3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551942.1">
        <property type="nucleotide sequence ID" value="XM_002551896.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCJ3"/>
    <dbReference type="FunCoup" id="C5DCJ3">
        <property type="interactions" value="295"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCJ3"/>
    <dbReference type="GeneID" id="8290775"/>
    <dbReference type="KEGG" id="lth:KLTH0B03520g"/>
    <dbReference type="eggNOG" id="KOG1488">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004017_6_1_1"/>
    <dbReference type="InParanoid" id="C5DCJ3"/>
    <dbReference type="OMA" id="VEYSKHK"/>
    <dbReference type="OrthoDB" id="668540at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000288">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07920">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000004">
        <property type="entry name" value="Pumilio homolog 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033133">
        <property type="entry name" value="PUM-HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033712">
        <property type="entry name" value="Pumilio_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537:SF12">
        <property type="entry name" value="MATERNAL PROTEIN PUMILIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537">
        <property type="entry name" value="RNA BINDING PROTEIN PUMILIO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00806">
        <property type="entry name" value="PUF"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50303">
        <property type="entry name" value="PUM_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="PUM-HD" evidence="9">
        <location>
            <begin position="393"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="7">
        <location>
            <begin position="414"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="7">
        <location>
            <begin position="450"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="7">
        <location>
            <begin position="486"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="7">
        <location>
            <begin position="522"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="7">
        <location>
            <begin position="558"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="7">
        <location>
            <begin position="594"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="7">
        <location>
            <begin position="684"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="151"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="277"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="316"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="16"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="318"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="375"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00060736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00081811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR21504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="756" mass="83314" checksum="D6C56BC92B9A8396" modified="2009-07-28" version="1">MSTTENQRDPWVTHDAAGSAGGAHAANMDSELASIVSSLSALSSVSAHQQHGQIGSFRRGSFHSNQGSDVESEMLFHTQTSPPIKRTTLSVGGMPLGGPSGVNNRLSKMKNYSGSLAGGSVLQQQPIQGGFFERFGRSLAEGTREVELNLGSTSGRASRRESMNAMDSLSRATTNSTFQGVAMEGRRTSVASDAMESLSENLNMQPEEPGNLNHRNIWKVAEAPVFRPQASENSGMEPAFPQPPAEALNQPYGFSYPNYWKMGAPMIPLAVPPSEQLKAGKDFPEPSAAGGNVPVDPSSQPFPPFMFPNNPYLFFPPGATPPPPPHPFQPAASNPKDLPKGPNAKTHGNPYLYNSRNHKPARGSPSAPFPPQPNPAAAANSSTPRGKGKYNVVRSPLLEEFRNNSSGKEYRLPDIYGSALEFCKDQHGSRFIQQELVKATDTEKEVIFNEIRDEAINLADDVFGNYVIQKYFEHGLKSQKDVLFEKFTGKMEKLSLQMYACRVIQRALECIDLDQKVILVNELSGCVLKMIKDQNGNHVIQKAIERIPIEKLPFILGSLEGQIYHLSTHSYGCRVVQRLLEYGTLEDQEKILADLDQFIPFLIQDQYGNYVIQHILKQGPETDDTHIGRTKQNIVDIVCKNVVEYSKHKFASNVVEKTMVFGSPAQKKQFMEKVLPESAEHATRLEDNAPLILMMRDQYANYVVQKLVGVAEGDDKKVTVIAIRSYLEKLNKINALGNRHLASVEKLAAIVENVQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCM3</accession>
    <name>C5DCM3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">V-type proton ATPase subunit a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0B04268g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Essential component of the vacuolar proton pump (V-ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the V-ATPase 116 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551972.1">
        <property type="nucleotide sequence ID" value="XM_002551926.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCM3"/>
    <dbReference type="FunCoup" id="C5DCM3">
        <property type="interactions" value="346"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCM3"/>
    <dbReference type="GeneID" id="8290809"/>
    <dbReference type="KEGG" id="lth:KLTH0B04268g"/>
    <dbReference type="eggNOG" id="KOG2189">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005230_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCM3"/>
    <dbReference type="OMA" id="TYVQLYI"/>
    <dbReference type="OrthoDB" id="10264220at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000220">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051117">
        <property type="term" value="F:ATPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002490">
        <property type="entry name" value="V-ATPase_116kDa_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026028">
        <property type="entry name" value="V-type_ATPase_116kDa_su_euka"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629:SF59">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT A, GOLGI ISOFORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629">
        <property type="entry name" value="VACUOLAR PROTON ATPASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01496">
        <property type="entry name" value="V_ATPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001293">
        <property type="entry name" value="ATP6V0A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 9" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="456"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="500"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="578"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="607"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="672"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="773"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="805"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="115"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR21534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="866" mass="98665" checksum="B1DFE79F06EB06EF" modified="2009-07-28" version="1">MTREEAIFRSADMTYVQLYIPLELSREVVCLLGNMGNVMFRDLNTDLTTFQRGYVNQIRKFDDLERLIDYMSNVASRYSEATWKYVFHGDNDNATQHPSMNFLMSSMHTHSLDTVSDLTAEITEFESRVRQLDESLVNLRKRLNTLIEHRHAAFECGRYMEVNPGLRGRAARSQQHRDLEQLTVEDFRLSDDEVSETLSDTFSFDEVSEDNSVPSAGRDLSAELEEGFRHRTTIIGSINRSKVETLNKILWRILRGNLFFHNIPIEEPLLEKGELVQKDCFVVFTHGDVLLKKVKRVVESLNGTIFPGNEGRSTIKNLNTQIADLQQICQTTEQTLHTELLIVSDQLPMWRAVARREKLIYAALNLFRQESQGLVAEGWLPSTELLIVSNALKNHSENIGSENSAVVSVIKTSKKPPTYHRTNKFTQSFQAIVDAYGIATYKEVNPGLATIVTFPFMFAIMFGDTGHGAIVFLIALFLVLNEKKLAQAQNGEIFDMAFSGRYVILLMGFFSIYTGLLYNDIFSLSMTFFKSGWKWNSGFKEGEAIEATNTGVYPFGLDYAWHGTENNLIFSNSYKMKLSILMGFIHMTYSLFFSLVNYRYFRSRVDIIGNFIPGFIFMQSIFGYLSWAIIYKWSKDWIKDGKVAPGLLNMLINMFLAPGVVDEPLFRGQSYLQVFLLLCALICVPWLLLYKPLKLRAQNKAAISNGYQNIHDQNASESLLESQEDAGGEILVTDYDENHSNTFEFGDIMIHQVIHTIEFCLNCISHTASYLRLWALSLAHAQLSSVLWTMTIANAFSSKDSGSPLAVIKVVFLFGMWFVLTVCILVLMEGTSAMLHSLRLHWVEAMSKFFEGDGYPYEPFSFKALD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDA9</accession>
    <name>C5DDA9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Eukaryotic translation initiation factor 3 subunit C</fullName>
            <shortName evidence="4">eIF3c</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Eukaryotic translation initiation factor 3 93 kDa subunit homolog</fullName>
            <shortName evidence="4">eIF3 p93</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Translation initiation factor eIF3, p93 subunit homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">NIP1</name>
        <name evidence="7" type="ordered locus">KLTH0B09680g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the eIF-3 subunit C family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552208.1">
        <property type="nucleotide sequence ID" value="XM_002552162.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDA9"/>
    <dbReference type="SMR" id="C5DDA9"/>
    <dbReference type="FunCoup" id="C5DDA9">
        <property type="interactions" value="1274"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDA9"/>
    <dbReference type="GeneID" id="8291051"/>
    <dbReference type="KEGG" id="lth:KLTH0B09680g"/>
    <dbReference type="eggNOG" id="KOG1076">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004304_0_2_1"/>
    <dbReference type="InParanoid" id="C5DDA9"/>
    <dbReference type="OMA" id="FRCGLIK"/>
    <dbReference type="OrthoDB" id="29647at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03002">
        <property type="entry name" value="eIF3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027516">
        <property type="entry name" value="EIF3C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008905">
        <property type="entry name" value="EIF3C_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13937">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITATION FACTOR 3, SUBUNIT 8 EIF3S8 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13937:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05470">
        <property type="entry name" value="eIF-3c_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="6">
        <location>
            <begin position="607"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="18"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR21770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="817" mass="92549" checksum="622754637B84F56B" modified="2009-07-28" version="1">MSRFFANSYDYESGSSSSEEDLLSTSEEELMSSSSSEEEQANDSFFDESESESDVDSDGSGDRPYGPDWFKKPEFRKGGSTGANKFLKNADYSNSEDSDDEGRKVVKSAKDKLLDEMRGVYDKIEDAEMTQDWITILAELDNMTRLSTRAQQQNYGVPNIFVKVLAQVEDAVAATSLQDINNKAVARAYNTTKQRVRKVARDYEQALSSFRENPEAHADDEAVQDNNSVSTFALAGKKTADLSSMATASSESDFFTALRIVIDSRGKKGTDFQAQIKTLEELLDIAKSPYETILAYLNLIPIRFDSSATLSYQPIDQWKASQQDIAKLFDTLEDSIDAYHVSELAPHNDFIEEEPQPNEQGIKQVLGSVYSFVERLDEEFNKSLLNTDPHSSDFLDRLKDEQGVYNLILRSQLYLEATSSDSELERRLARVLTRRFDHIYFKSVKLVEVSESKAWKSLREGAKSKVTPLDGEVDESYTFGLIDTLHKMLSTQKDGAQRRRAGLYQIYFYALNNQFEKAKHMLLSSHIQSSINNADAPLQILFNRTVVQLGLAAFKLCLIDDCHQILNEVSTSTHLRDILGQQSLQRVAANNATNPGGASNEQLCLPFHQHINLDLIDVVFMTCSLLIEVPHMAAFFSGIKVKRIPYSQKSIRRALEHYDKSSFQGPPETLRDHVLYAAKSMQRGDWDKSVEYLKKVPTWSLLPNTAEVLTNLAHRVQVEALRTYIFTYKRFYSKLSLLKLSQLFGLSQETVVEVVSAIISQYDIKGRLDEESKFLVFEKGYEITKLEEVAVKLAKETKYVSERLNEKKYVGSSRKQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDD0</accession>
    <name>C5DDD0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0B10252p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0B10252g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552229.1">
        <property type="nucleotide sequence ID" value="XM_002552183.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDD0"/>
    <dbReference type="FunCoup" id="C5DDD0">
        <property type="interactions" value="186"/>
    </dbReference>
    <dbReference type="GeneID" id="8291077"/>
    <dbReference type="KEGG" id="lth:KLTH0B10252g"/>
    <dbReference type="eggNOG" id="ENOG502SAGH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081384_1_0_1"/>
    <dbReference type="InParanoid" id="C5DDD0"/>
    <dbReference type="OMA" id="WNEIANI"/>
    <dbReference type="OrthoDB" id="4036517at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001142">
        <property type="entry name" value="DUP/COS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00674">
        <property type="entry name" value="DUP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="223" mass="25823" checksum="C16888E284FB1F4A" modified="2009-07-28" version="1">MMREERVEEYSLHASQEDDQSLTLPRDIYPNKFLYQISRHRAPLFATSLELVLCTLLIRYFMKKNDSSARFEAVSSVVSFGLPLGLSLFSGMVHLVHNCGKAPVNRVRFMEEIIAVEPGTETRKWDSIAARLNAIFYSNNSLATPHFFYDGNACYFYFRLHYVRPLFEKKESSYAFREFPDEFKALIHQAMEAYKKGLDKDWQRLLNTGSSLGIRASTSEKMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDL9</accession>
    <name>C5DDL9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0C02068p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0C02068g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552318.1">
        <property type="nucleotide sequence ID" value="XM_002552272.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDL9"/>
    <dbReference type="FunCoup" id="C5DDL9">
        <property type="interactions" value="102"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDL9"/>
    <dbReference type="GeneID" id="8291177"/>
    <dbReference type="KEGG" id="lth:KLTH0C02068g"/>
    <dbReference type="eggNOG" id="KOG1183">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007914_3_0_1"/>
    <dbReference type="InParanoid" id="C5DDL9"/>
    <dbReference type="OMA" id="CFWPVQY"/>
    <dbReference type="OrthoDB" id="70250at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007720">
        <property type="entry name" value="PigQ/GPI1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21329:SF3">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21329">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT Q-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05024">
        <property type="entry name" value="Gpi1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="381"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="413"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="450"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="605" mass="69360" checksum="5CC76368382DFD49" modified="2009-07-28" version="1">MKYIYWPKVAGFNKNDARIVVALQAPESDLIVISLLTARETKLLQKSNRFKRIGHIDGNNEFVSELDEKDICIVEFWPPKLNLMQFYALEPISLALPEKADSTLLSRATGGQPSPTEKFQMRESINSINLYYKHLQSLRALIGRAGPSNRGIPVRRIARALADLCGLLPLLSALRQALTYMIIFIHSFARFLSAVLNWNPLCLVSFSATAQQIDLRSQQFCYFPVQYLRITENAVFNRMRPRSELSVCHPKEKQETRGNASAGVFPCEYYPDYIRFYNTIWLIVNDVSFGLTVGTLLAENKLRIAKFINFHLTDYLFHKVHYITAYLGQNPLGIKLNGELAHFLSELFLWIIDFSYSTYIRRILDVSSIQTVISIVSRTSCCFGVTFALSLTVDLMSVLSMHISLFYYISAKMYHWQLHVMRSLLYLFWGKKRNLLRKRVDSNMFELDQLVMGTLFFTMMVFLLPTLAVFYISFTALRMTILMPELLLESLMALLNHFPLFVLLLRLKDPSRIPGGVSMEALGSKNRFALQNNPLTVSSMFRPYSLLMGMMAENYCSVATLKQILVGRPITIKRYKMYQVLYSALPKVPIATSKLWEALKGSRPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDQ8</accession>
    <name>C5DDQ8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0C02992p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0C02992g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552357.1">
        <property type="nucleotide sequence ID" value="XM_002552311.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDQ8"/>
    <dbReference type="FunCoup" id="C5DDQ8">
        <property type="interactions" value="361"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDQ8"/>
    <dbReference type="GeneID" id="8291219"/>
    <dbReference type="KEGG" id="lth:KLTH0C02992g"/>
    <dbReference type="eggNOG" id="ENOG502R6H5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037140_0_0_1"/>
    <dbReference type="InParanoid" id="C5DDQ8"/>
    <dbReference type="OMA" id="QEIDHNT"/>
    <dbReference type="OrthoDB" id="19159at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990334">
        <property type="term" value="C:Bfa1-Bub2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044732">
        <property type="term" value="C:mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031578">
        <property type="term" value="P:mitotic spindle orientation checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034586">
        <property type="entry name" value="Bfa1/Byr4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35140">
        <property type="entry name" value="MITOTIC CHECK POINT PROTEIN BFA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35140:SF1">
        <property type="entry name" value="MITOTIC CHECK POINT PROTEIN BFA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="359"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="421"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="42"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="174"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="452"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="535" mass="61496" checksum="16EAA8A3D237733E" modified="2009-07-28" version="1">MSIRPVNIDEFTETSFEDIEASVSPTNWKKRSQQSSPLRDVAAAPSTASSESTTFSNDKKRYSTHTESDEDGGAFLDDFEEFHGKASRGEIVKSHFENVNLEPTFRPSTEAQESEGDDELQELTRRFGNKFDIKSRHLSGAQRPRSAFKTFKQPQSMMDLRESTTRPSSRSSHELTNSKSATNLRSGSRLGPRGIQYKKSVPALSKYDMIREEDEQDNYNDEFEDELKFEKSLLQPQFLPKEDEGSLLDLSPSQYTITADDSILTPQLHKRHRDWVRPSQLEMFREPLRHEKPAVKHRDSRKLSVSHRLKTIKQEIDHNTPMKKGRMVYNPETLKWEGNEQALAKFRDVDSFDKKALVIQGRPDLQPPRERSSSTRSGTRKHGKVVGRMMFDQDNLRWIRLDGDEKDPFAEIPDYIPPVSFSEPSLKKKLPTKRSQSQFAPSRDQCGGRYPSSATTRYHSLNTPSSSSDPTFNIDSKSLERFYHEENKWSKKVGGWFILRDAEAEGYGADQELPDPKNNSYMYEIRNMVMSSARN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDQ9</accession>
    <name>C5DDQ9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0C03014p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0C03014g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the PRP39 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552358.1">
        <property type="nucleotide sequence ID" value="XM_002552312.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDQ9"/>
    <dbReference type="FunCoup" id="C5DDQ9">
        <property type="interactions" value="215"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDQ9"/>
    <dbReference type="GeneID" id="8291220"/>
    <dbReference type="KEGG" id="lth:KLTH0C03014g"/>
    <dbReference type="eggNOG" id="KOG1258">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024449_0_0_1"/>
    <dbReference type="InParanoid" id="C5DDQ9"/>
    <dbReference type="OMA" id="SLELWCD"/>
    <dbReference type="OrthoDB" id="10265668at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000243">
        <property type="term" value="C:commitment complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030627">
        <property type="term" value="F:pre-mRNA 5'-splice site binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000395">
        <property type="term" value="P:mRNA 5'-splice site recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059164">
        <property type="entry name" value="HAT_PRP39_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17204">
        <property type="entry name" value="PRE-MRNA PROCESSING PROTEIN PRP39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17204:SF5">
        <property type="entry name" value="PRE-MRNA-PROCESSING FACTOR 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23241">
        <property type="entry name" value="HAT_PRP39_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23240">
        <property type="entry name" value="HAT_PRP39_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR21920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="674" mass="79181" checksum="F34A1FBA4E4A363C" modified="2009-07-28" version="1">MFYLFTLSGIYDHFLFTTCNDFYLQTSISRKDNRREAQPMDLRTSMDEALDTKTGPKGEEVPESIFEGLEVGFLKDNEQWVSKVKALNWDDVASLEEMVKATEQLVLKYSNPNQHIKHSIKKVFEKVLGRYPLLFGYWKRFTAVQYQLNGLQSSIDVLSQAVDAFPNSLELWCDYLSVLLANNPDQVDQIRVNFLIAKDLVGLQFLSHPFWDKFIEFESRHQQWKNVLAIYREVSKIPLHQYSKYYTAYKQLAERTDTGIRIEEDIDQVFANTQKLVNEVWTYESQIAQSFFNIGPVRQKELDNWDQYLEFVINSEHFHASLVKSTFMRCLVPCRNYEHFWLRFTKWMEENSSLEECLDVYQKGVNAISQEMRTLRQQFLDYMKLRLKDDEDKVLELYLLTLSEFSRIYQQDASFMVEFLAAVRRTSFSSDLSQTDQEILRQQTSYASYLEKNVRCYTNQTPDGDVKLQGILNDKNLSVAVVELIKVTHLNLRNVSQSRKYFKEFESLPQIRNSTAFWLLYYKILKVSVELNELERFVARLGTEIFLPTLVVNDILDDFKSFFLTNVNYPDYEHEVYKGRYTSGIDPLTDLYFKVNNPLWTKQKSTDRQRNEIKDNGHIGLYLEKPEISNTIIESHSKSFSNGPPSLPTFKNLDKANKQASFKDFLSTDYTNVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDR8</accession>
    <name>C5DDR8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pre-mRNA-splicing factor ISY1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Pre-mRNA-splicing factor isy1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0C03256g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ISY1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552367.1">
        <property type="nucleotide sequence ID" value="XM_002552321.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDR8"/>
    <dbReference type="FunCoup" id="C5DDR8">
        <property type="interactions" value="269"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDR8"/>
    <dbReference type="GeneID" id="8291231"/>
    <dbReference type="KEGG" id="lth:KLTH0C03256g"/>
    <dbReference type="eggNOG" id="KOG3068">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043453_2_1_1"/>
    <dbReference type="InParanoid" id="C5DDR8"/>
    <dbReference type="OMA" id="WEHHIKS"/>
    <dbReference type="OrthoDB" id="1739576at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005684">
        <property type="term" value="C:U2-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000350">
        <property type="term" value="P:generation of catalytic spliceosome for second transesterification step"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.660:FF:000001">
        <property type="entry name" value="pre-mRNA-splicing factor ISY1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.660">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029012">
        <property type="entry name" value="Helix_hairpin_bin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009360">
        <property type="entry name" value="Isy1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037200">
        <property type="entry name" value="Isy1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13021">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR ISY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06246">
        <property type="entry name" value="Isy1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140102">
        <property type="entry name" value="ISY1 domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00073166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR21929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="266" mass="31101" checksum="B71A695B0F67E6DA" modified="2009-07-28" version="1">MSRNVDKANSVLVRFQELEAEKNGGYKDYTRFKRPTRVGSVSKSQEAQQWRRQVISDINTRLTRMHDPSLNEFQLRELNDELNDLFSEKARWERHIHRNLKGPDFSRDKSFKHTGTVLDGRRYFGRALALPEVQQLAKNAADWRKKSQSARQRQKTLQDRVRVWKKSLGPEYYGFVPEDSASLRVPLHLTVETVKEILTSKPASRSHFCQLEEDKTQELQAEAGGQGSVMAPKRIIPQFSQLPDLKDMEEWLVQRRKSQLQRQLNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDY4</accession>
    <name>C5DDY4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">amidase</fullName>
            <ecNumber evidence="3">3.5.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0C04796g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a monocarboxylic acid amide + H2O = a monocarboxylate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:12020"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:35757"/>
            <dbReference type="ChEBI" id="CHEBI:83628"/>
            <dbReference type="EC" id="3.5.1.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amidase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.4" evidence="3"/>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552433.1">
        <property type="nucleotide sequence ID" value="XM_002552387.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDY4"/>
    <dbReference type="FunCoup" id="C5DDY4">
        <property type="interactions" value="73"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDY4"/>
    <dbReference type="GeneID" id="8291300"/>
    <dbReference type="KEGG" id="lth:KLTH0C04796g"/>
    <dbReference type="eggNOG" id="KOG1212">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009600_9_2_1"/>
    <dbReference type="InParanoid" id="C5DDY4"/>
    <dbReference type="OMA" id="GAKCSVI"/>
    <dbReference type="OrthoDB" id="6428749at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004040">
        <property type="term" value="F:amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020556">
        <property type="entry name" value="Amidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46072:SF4">
        <property type="entry name" value="AMIDASE C550.07-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46072">
        <property type="entry name" value="AMIDASE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001221">
        <property type="entry name" value="Amidase_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00571">
        <property type="entry name" value="AMIDASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="7">
        <location>
            <begin position="97"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="152"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="227"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="5">
        <location>
            <position position="251"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="248"/>
            <end position="251"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001221-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001221-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR21995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="569" mass="63473" checksum="A691D5DC7F58F0E8" modified="2009-07-28" version="1">MTQQKFPAQDAAKFSKYEPLIEEYKKEAVAQMQQFDPAFYEKCVATIPSENELDTEPIDARQFLSFLTNEEASIVNDLTISDLTRKQLDGELTATVITKAFIKAAIIAQLATNCVMQFLIPEALARAQELDDYLKENKKLVGPLHGVVVSLKEHMNFKGKVTNASYVAYLENIPDNEAVSITILRKLGAVFHTRTAQPQGIMHLDTWNNITGRTRNPLSTRLSPGGSSGGESAIVAMHGSVIGHGSDIGGSVRAPAAFTDIFGLRPTTKRISLLNGLSSGKGQESIVAVQGPLARSIDELDAYMRAYINEGQPWTYDPNCIPIAWREPSLPPKIKIGVLYDDNLVTPFPAVTRAISETVAKLSKNPAFEIVNLKPDWFSEEEMLDIYTANLKLYTCDGNEIQLSMWTPSGEPLLPLTEHYMKFGGGKKLSIYENRMLNAQRDALKLQMQQKYFSELDFILSPTYNGPSEIPGESLYWGYTSFWNLLDYPNVTFPVDVAHDKEKDAGIPELRKNEYEKMVWYKENGDVRYDPESYIGGPVALQLTGKRFCDEDVVAATKQIISALGVERR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDZ9</accession>
    <name>C5DDZ9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0C05126p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0C05126g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552448.1">
        <property type="nucleotide sequence ID" value="XM_002552402.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDZ9"/>
    <dbReference type="FunCoup" id="C5DDZ9">
        <property type="interactions" value="99"/>
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    <dbReference type="eggNOG" id="ENOG502QQ8J">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033323_3_3_1"/>
    <dbReference type="InParanoid" id="C5DDZ9"/>
    <dbReference type="OMA" id="MRLFCMR"/>
    <dbReference type="OrthoDB" id="10261749at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="CDD" id="cd07067">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="His_Pase_superF_clade-1"/>
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        <property type="entry name" value="His_PPase_superfam"/>
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        <property type="entry name" value="PG/BPGM_mutase_AS"/>
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        <property type="entry name" value="PGM-Related"/>
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        <property type="entry name" value="BROAD-RANGE ACID PHOSPHATASE DET1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <feature type="binding site" evidence="1">
        <location>
            <begin position="13"/>
            <end position="20"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="310" mass="36663" checksum="11CF79EE2EF25E56" modified="2009-07-28" version="1">MGHHKPRLIILVRHGESVSNKDKSVNQHMPNHLIPLTDNGWKQARRSGSELLQLLNVDDPQIVKDLAEKFAWEGDHRKPLKQDYRNVNKNLDSNIVFYTSPYRRTRETLRGMLDVLDEYNKRNCGVSARDNDGYQPSVKSRLAHWQCPEFPHGFYENTESRDLIASLNRTAAHKTYVHYRVKDEPRIREQDFGNFQELSSMRDVMSTRANYGHFFFRFPQGESAADVYDRCSSFQETLFRHFNQPDRKPRDVVVLVTHGIYLRVFLMKWFRWTYEEFEAFTNVPNGSLVVMELDTELDRYVLRTTLPKWC</sequence>
</entry>
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    <accession>C5DE42</accession>
    <name>C5DE42_LACTC</name>
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        <submittedName>
            <fullName evidence="1">KLTH0C06116p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0C06116g</name>
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    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
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                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
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                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
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                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
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                <person name="Talla E."/>
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            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="CAR22053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="KEGG" id="lth:KLTH0C06116g"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2237083_0_0_1"/>
    <dbReference type="InParanoid" id="C5DE42"/>
    <dbReference type="OMA" id="TWCICRT"/>
    <dbReference type="OrthoDB" id="4028543at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR22053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="105" mass="12266" checksum="1646BE57C032B7CB" modified="2009-07-28" version="1">MIRDPLSRKRTPRFFKKTPTIQFLKRVESRPHRLKRFVRVSVLGPLVIRYGALHGTPDLRPIKCRYRGINHGDLVQPGTWCICRTLSLRPSPLHNDISKIMEKVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DE78</accession>
    <name>C5DE78_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0C06952p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0C06952g</name>
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    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RICTOR family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002552481.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DE78">
        <property type="interactions" value="544"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DE78"/>
    <dbReference type="GeneID" id="8291396"/>
    <dbReference type="KEGG" id="lth:KLTH0C06952g"/>
    <dbReference type="eggNOG" id="KOG3694">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001013_1_1_1"/>
    <dbReference type="InParanoid" id="C5DE78"/>
    <dbReference type="OMA" id="EIRIHAT"/>
    <dbReference type="OrthoDB" id="271111at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031932">
        <property type="term" value="C:TORC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038203">
        <property type="term" value="P:TORC2 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028268">
        <property type="entry name" value="Pianissimo_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028267">
        <property type="entry name" value="Pianissimo_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029453">
        <property type="entry name" value="Rictor_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029451">
        <property type="entry name" value="RICTOR_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029452">
        <property type="entry name" value="RICTOR_V"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13298">
        <property type="entry name" value="CYTOSOLIC REGULATOR PIANISSIMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13298:SF11">
        <property type="entry name" value="RAPAMYCIN-INSENSITIVE COMPANION OF MTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14663">
        <property type="entry name" value="RasGEF_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14666">
        <property type="entry name" value="RICTOR_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14664">
        <property type="entry name" value="RICTOR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14668">
        <property type="entry name" value="RICTOR_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01303">
        <property type="entry name" value="RasGEF_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01307">
        <property type="entry name" value="RICTOR_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01308">
        <property type="entry name" value="RICTOR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01310">
        <property type="entry name" value="RICTOR_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rapamycin-insensitive companion of mTOR N-terminal" evidence="5">
        <location>
            <begin position="246"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="domain" description="Rapamycin-insensitive companion of mTOR middle" evidence="4">
        <location>
            <begin position="661"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="domain" description="Rapamycin-insensitive companion of mTOR" evidence="6">
        <location>
            <begin position="1069"/>
            <end position="1141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="140"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="1197"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="27"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="141"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="203"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR22089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1329" mass="150909" checksum="0DEE1F1D013239F4" modified="2009-07-28" version="1">MTSQRSSPHLTRSRSSADEILTRSRHNTVLSTSFVSTRRADEQPASPLSAHKPRRTRSGTAKSINNLKHDIHHVQQDLVRLRKSKEDTEKLKESATNDIYPGNYSQEHLQRHSMVLQSNNQLRKLDQQIKKSGDLLSSLRKKLDSTTEEVRPTASTNPSFISKELRRTSDYTTADDSTTAEELSARSLSEDDVSSASLDEASNGESPGNKQVSSSDAAASEKHATWLVSDYLQSLQDKNASTDYLLETANDLTLLLKQNPTIKFDLYLPAFSSTIQQLLLSDADVTVAAAYRICRHLITGQEFIQELLKLHLEPFLIISLAKPNSCYVERLHALKLIRSFLGHDAGITLGLVQAIISCIEKPDDRLREVALETLLELCYTKPMFVRRCGGIQFLGSFIGECSSPQQAAYVLDAMLDLMTFRETRSCFIDSFDVRVVLTSLSDSQIKANFNAERLKSGINLVSRCLTNYNGIILFSQDDFRPLRELLAFFQAPQLASYLVDLFLDVLRIRSISPTGKKTANHNFKISPSHFHFETMPINQYVGLLAGVLYKVGFIDCLLGVLIQLAPGKKDHGVAAKTRYLITEYCNIAMNLAGLKPSASAGLLSFNYSDFEKVFSEVFQYEKVTNKLNKSRNTIGMSDIGSLRDIMDFSEQLKHKALINDVDEARFKRMVYDTRVLQTKDFSLWNWSILSDLLEGPLLNGKRLDDLARTTKFFRRLMVFYRPMRFRFSKIRQGSRLAPKCVHVGRQLFRTLTSNREGIKVLSDDTKLIPQIASLLFKAMEGHTAGNVFSRESLSKTVCAGYFKIIGTFTESLRGVRILEKWNVFTVIYKMFQHTSKFTDSFLLLILPELDLEHSLHCRNILNKALVHPMEEVRVEATRLLGNKIQDIPQKASTSGREKVLEKFLVELLVRQLYDLSPKVIAAADKILYDYCATQDWPPRVDIPRHHLLDQLMFIRSPILLEFLKTPAGFKQLNDIGFVETEREKWISHKNKEYVSRVEAFIQAEASKFSNDTSYSTPSRKKLPMHFCHSLASTEEGVNLISQKGDFVQIINIIKRYRHNNFPDLTVDEDIELKAALWCCGHIGSTEPGINLLDNYSIVNDIVSISLRTSSMSIKFTSFFVLGLVAKTNEGCEILDELGWDCSLNVQKVPLGLTFPRDVLRFLRFPESNLSDDRFADQGTQINDGDAPLEYEVPTLDLDMLLNNKAKAENTMGDAQEELQAEIERQTRNLEMFRLQACQISSDEALDRVMRAASKLNNHILCNAAVKEITEVISTYGAARFETPKVFFWVLGLLEQYRFKPQARKFLCDLLLRKKTLEVMIRKDKKKRKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DE89</accession>
    <name>C5DE89_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">coproporphyrinogen oxidase</fullName>
            <ecNumber evidence="4">1.3.3.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0C07194g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; protoporphyrinogen-IX from coproporphyrinogen-III (O2 route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the aerobic coproporphyrinogen-III oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.3.3" evidence="4"/>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552538.1">
        <property type="nucleotide sequence ID" value="XM_002552492.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DE89"/>
    <dbReference type="FunCoup" id="C5DE89">
        <property type="interactions" value="626"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DE89"/>
    <dbReference type="GeneID" id="8291407"/>
    <dbReference type="KEGG" id="lth:KLTH0C07194g"/>
    <dbReference type="eggNOG" id="KOG1518">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026169_0_0_1"/>
    <dbReference type="InParanoid" id="C5DE89"/>
    <dbReference type="OMA" id="VHANWRY"/>
    <dbReference type="OrthoDB" id="15318at2759"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00322"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004109">
        <property type="term" value="F:coproporphyrinogen oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1500.10:FF:000006">
        <property type="entry name" value="Coproporphyrinogen III oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1500.10">
        <property type="entry name" value="Coproporphyrinogen III oxidase, aerobic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001260">
        <property type="entry name" value="Coprogen_oxidase_aer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036406">
        <property type="entry name" value="Coprogen_oxidase_aer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018375">
        <property type="entry name" value="Coprogen_oxidase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003727">
        <property type="entry name" value="PRK05330.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10755">
        <property type="entry name" value="COPROPORPHYRINOGEN III OXIDASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10755:SF0">
        <property type="entry name" value="OXYGEN-DEPENDENT COPROPORPHYRINOGEN-III OXIDASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01218">
        <property type="entry name" value="Coprogen_oxidas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000166">
        <property type="entry name" value="Coproporphyri_ox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00073">
        <property type="entry name" value="COPRGNOXDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102886">
        <property type="entry name" value="Coproporphyrinogen III oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01021">
        <property type="entry name" value="COPROGEN_OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR22100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="327" mass="37562" checksum="D16366EDFD7B52E1" modified="2009-07-28" version="1">MPAPEDPTHLPIRKQMEELIRRKQREITQGLETLDTVKFTPDSWTRGNDGGGGTSMVLQNGTTFEKGGVNVSVVYGQLSPAAISAMKHDHKNLKLPEDPATGLPNTEGVKFFACGLSMVIHPSNPHAPTTHLNYRYFETWNPDGTPQTWWFGGGADLTPSYLYEDDARLFHEMHKKALDKHDAALYPRYKKWCDEYFFIKHRNETRGIGGIFFDDMDDRDPQEILSIVEDCFDAFLPSYLPIIARRKDMPFTEEEKQWQLIRRGRYVEFNLVLDRGTQFGLRTPGSRTESILMSLPVTACWFYDHHPEPGSREDKLLKVLENPVDWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEA6</accession>
    <name>C5DEA6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0C07634p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0C07634g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552555.1">
        <property type="nucleotide sequence ID" value="XM_002552509.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEA6"/>
    <dbReference type="FunCoup" id="C5DEA6">
        <property type="interactions" value="129"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEA6"/>
    <dbReference type="GeneID" id="8291427"/>
    <dbReference type="KEGG" id="lth:KLTH0C07634g"/>
    <dbReference type="eggNOG" id="KOG4155">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042821_0_0_1"/>
    <dbReference type="InParanoid" id="C5DEA6"/>
    <dbReference type="OMA" id="WNSEGSE"/>
    <dbReference type="OrthoDB" id="361494at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031464">
        <property type="term" value="C:Cul4A-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000109">
        <property type="term" value="C:nucleotide-excision repair complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006283">
        <property type="term" value="P:transcription-coupled nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042238">
        <property type="entry name" value="Rad28/ERCC8/Ckn1/ATCSA-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46202">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46202:SF1">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="50"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="138"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="247"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="96"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR22117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="425" mass="47336" checksum="ADCE54BAA53463F8" modified="2009-07-28" version="1">MNRTQLQWQLGKLDNRRLYDLVLCSELEKLRDAKPIVYSHRKASFPIGTTSLDFDSTGQFLLSSGEDGSLALWALDEGHSEGKLVNKRIQFAAGESKESVNPSSRRVHVTARSPGGKAPRFLTSVESRPPDSLQGPREASHAFSVTSVKWYGPDNGLFFTGSNDHKVKIWDTNTFQVASSIDIAHRVAQLDTLGDLLAVASEDSHPRLIDLRSMSAAISLGVKRSDMRHGINAAKFSHTSSGAKPLLLATGDDDGNVRVWDLRMGNRWLCDLTEADTLSKSHARCCNDLCWDPSGSLKLVTTGNDGKCKMWDLHEGGAKPQLVRQLGATDLTSNRFRRRTSHYLMWQGPYVFFNSDHGEILVYQSHSGLLWHAFEYPLRVAQPERLRSTPKLQSMAIQTRLANTLGVRLVLGTDNTHGRILEYRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEF6</accession>
    <name>C5DEF6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0C08778p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0C08778g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552605.1">
        <property type="nucleotide sequence ID" value="XM_002552559.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEF6"/>
    <dbReference type="FunCoup" id="C5DEF6">
        <property type="interactions" value="111"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEF6"/>
    <dbReference type="GeneID" id="8291479"/>
    <dbReference type="KEGG" id="lth:KLTH0C08778g"/>
    <dbReference type="eggNOG" id="KOG0565">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025224_2_0_1"/>
    <dbReference type="InParanoid" id="C5DEF6"/>
    <dbReference type="OMA" id="ITIWARC"/>
    <dbReference type="OrthoDB" id="62798at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004439">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046985">
        <property type="entry name" value="IP5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000300">
        <property type="entry name" value="IPPc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200">
        <property type="entry name" value="INOSITOL 5-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200:SF275">
        <property type="entry name" value="LD06095P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22669">
        <property type="entry name" value="Exo_endo_phos2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00128">
        <property type="entry name" value="IPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="354"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="Inositol polyphosphate-related phosphatase" evidence="2">
        <location>
            <begin position="3"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR22167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="372" mass="41731" checksum="4583F867C8DD04C2" modified="2009-07-28" version="1">METKYKLFVSTFNCGKCLHFEQTSEIDSILSHIIPKDHVHDIYALGFQELVPLWQGSFPDEVHAQLQSLSSRVLNLINSLNPRRSFRLVGLNSVGAIGLLLFADTTYEVTSILKASARCGALCSSLKGATAIKFNLSRPSSSATDSFIFIAAHFAANEGELQRQRREGDYNNIVGTLQQELGCFEGNHVLFCGDFNFRSRKWASTPADFNNLELLQAAVGVHDELSLSRKAERVFQNFEEAPITFAPTYKFKLSVPDPEYNLRRIPSWCDRILFQNYGSPVKILSYAACKREAVLQFTDHLPVVLQLEVPHLSPRSPTRSKALSSQSRSYKLMGDVADFLIGYGGWAYVFHGHWLLAVSLVLGLLVMFHLFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEN5</accession>
    <name>C5DEN5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription initiation factor TFIID subunit 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">TBP-associated factor 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0C10714g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Functions as a component of the DNA-binding general transcription factor complex TFIID. Binding of TFIID to a promoter (with or without TATA element) is the initial step in pre-initiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552684.1">
        <property type="nucleotide sequence ID" value="XM_002552638.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEN5"/>
    <dbReference type="FunCoup" id="C5DEN5">
        <property type="interactions" value="389"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEN5"/>
    <dbReference type="GeneID" id="8291566"/>
    <dbReference type="KEGG" id="lth:KLTH0C10714g"/>
    <dbReference type="eggNOG" id="KOG2341">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036634_0_0_1"/>
    <dbReference type="InParanoid" id="C5DEN5"/>
    <dbReference type="OMA" id="YGWLTSS"/>
    <dbReference type="OrthoDB" id="21060at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08045">
        <property type="entry name" value="HFD_TAF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045144">
        <property type="entry name" value="TAF4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007900">
        <property type="entry name" value="TAF4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15138">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15138:SF14">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05236">
        <property type="entry name" value="TAF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription initiation factor TFIID component TAF4 C-terminal" evidence="10">
        <location>
            <begin position="162"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="27"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="44"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="109"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="141"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR22246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="410" mass="44995" checksum="DB19EB622AA0B4FA" modified="2009-07-28" version="1">MARSPKRQDPNAQENLRAKRPKKEEQEQIFDEEEPAFEIGGGNTNESASPENTDFSTNMPTPFDNIVEDPVGGASLPESASPTTNLAQTTNSNSQSVKDVDDKKRGRPSGSQTQNTKVNSTGPKKSVMTQSSASAHHPNTQQAQSQNQQAASSKMQTDPDKLSDALFSAGVDIREEEALLNSSMNITKSSAQAGSQAVNNQIPPQPPLLHPTHVASFMKKVGSEQNFNQDFSKAHDVLSLMSTACEVFMTDIITNSLVISRHRRRSIKLNSGRRSEVSRALRDLAIHQKEQEERRVKRRIAMGLEKETAEAKMETEETLHRASNATANMMIAGGKKKYSWLNAGAKANNNSVKVLGKVSSDVAARGEMGIRYREAREEPGIVMRDLLNALENRRVGVNTVVAKGYARIRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEQ1</accession>
    <name>C5DEQ1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0C11132p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0C11132g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
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        <property type="protein sequence ID" value="CAR22262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_002552654.1"/>
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        <property type="interactions" value="1008"/>
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    <dbReference type="STRING" id="559295.C5DEQ1"/>
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    <dbReference type="KEGG" id="lth:KLTH0C11132g"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="HOGENOM" id="CLU_041217_10_6_1"/>
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        <property type="component" value="Chromosome C"/>
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        <property type="term" value="C:fungal-type vacuole membrane"/>
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        <property type="term" value="C:late endosome"/>
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        <property type="term" value="F:GTP binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:GTPase activity"/>
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        <property type="term" value="P:regulation of vacuole fusion, non-autophagic"/>
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        <property type="entry name" value="Rab7"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ras-related small GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="P-loop_NTPase"/>
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        <property type="entry name" value="Small_GTP-bd"/>
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        <property type="entry name" value="small_GTP"/>
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        <property type="entry name" value="RAB FAMILY"/>
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        <property type="entry name" value="RAS-RELATED PROTEIN RAB-7A"/>
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        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
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    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
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        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RAN"/>
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        <property type="entry name" value="RAS"/>
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        <property type="entry name" value="RHO"/>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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        <property type="entry name" value="RHO"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0636">Prenylation</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR22262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="208" mass="22930" checksum="5238E8C5C75DB800" modified="2009-07-28" version="1">MSSRKKNILKVIILGDSGVGKTSLMHRYVNDKFSQQYKATIGADFLTKEVTMDGDKVATMQVWDTAGQERFQSLGVAFYRGADCCVLVYDVTNAKSFENIKSWRDEFLVHANVSSPETFPFVILGNKLDVEESKKVVSNRSAQDMAKSLGNIPLFLTSAKGSINVDTAFEEIARNALQQSQADADAFEDDFNDAINIQLDGEPSSCSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFF4</accession>
    <name>C5DFF4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0D14652p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0D14652g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002553301.1"/>
    </dbReference>
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        <property type="interactions" value="637"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFF4"/>
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    <dbReference type="KEGG" id="lth:KLTH0D14652g"/>
    <dbReference type="eggNOG" id="KOG1474">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001499_4_0_1"/>
    <dbReference type="InParanoid" id="C5DFF4"/>
    <dbReference type="OMA" id="KMNIPHY"/>
    <dbReference type="OrthoDB" id="784962at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
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        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:chromatin remodeling"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Bromo_BDF1_2_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05499">
        <property type="entry name" value="Bromo_BDF1_2_II"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="Bromo_chromatin_reader"/>
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        <property type="entry name" value="Bromodomain"/>
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        <property type="entry name" value="Bromodomain-like_sf"/>
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        <property type="entry name" value="Bromodomain_CS"/>
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        <property type="entry name" value="NET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038336">
        <property type="entry name" value="NET_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22880:SF225">
        <property type="entry name" value="BROMODOMAIN-CONTAINING PROTEIN BET-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22880">
        <property type="entry name" value="FALZ-RELATED BROMODOMAIN-CONTAINING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51525">
        <property type="entry name" value="NET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bromo" evidence="4">
        <location>
            <begin position="114"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="4">
        <location>
            <begin position="280"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="NET" evidence="5">
        <location>
            <begin position="467"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="202"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="232"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="369"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="437"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="547"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="605"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="19"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="79"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="232"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="373"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="439"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="556"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="616"/>
            <end position="634"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR22909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="634" mass="70040" checksum="9FC065371375D270" modified="2009-07-28" version="1">MTSEVKTEVNGDGAKAREAAAPAGAPAAAPEGAPAGTPASAPAGGSSDAAPGAPADTPAGAPADASTKTPAGDGSPVLTPAPAPPPEPDMNNLPADPMPKHQHKHATTAIKAVKRLKDARPFLQPVDPVALNVPLYYNYIKRPMDLSTIERKLTANAYAQPEEVSADFKLMVENCARFNGESSAIAQMGRNIEASFEKHMLGMPTRDAPPRPRAKRRRSELETPVVIRRAESHNGRPKREIHPPRSKDIYPYETAKPRSKKHQGELKFAQQVVKELMSKKYSSFNYPFLEPVDPVALNCPTYFDYVKEPMDLGTVSRKLNNWEYENADQVEVDIRLVFKNCYAFNPDGTIVNMMGHRLEDVFNTRWVDRPVVPDEESDEEEEDAESDYSSDENLRAGADDDIDESSITNPAIQYLEQQLERMKVELQQLKTQELERIRKERRLARGSRKRRTGRKKSRSTDAAASGKRKKSKLKAVVTYDMKKIISERINDLPPSKLEKAVDLIRKSMPDIGKDDEVELDIDMLDNTTILTLFNTFFREYATSNGNGSVADESLRRSSMSPGSASNAKKTGRRRSKALSEQEQNKQIEKIKSKLAILDGASPTNSNAFNALLAGDDSSDDGDDDDDLSSESEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFH1</accession>
    <name>C5DFH1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0D15048p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0D15048g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SRR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553364.1">
        <property type="nucleotide sequence ID" value="XM_002553318.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFH1"/>
    <dbReference type="FunCoup" id="C5DFH1">
        <property type="interactions" value="83"/>
    </dbReference>
    <dbReference type="GeneID" id="8295608"/>
    <dbReference type="KEGG" id="lth:KLTH0D15048g"/>
    <dbReference type="eggNOG" id="KOG3131">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084828_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFH1"/>
    <dbReference type="OMA" id="THIRCLA"/>
    <dbReference type="OrthoDB" id="551431at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012942">
        <property type="entry name" value="SRR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040044">
        <property type="entry name" value="SRR1L"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28626">
        <property type="entry name" value="SRR1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28626:SF3">
        <property type="entry name" value="SRR1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07985">
        <property type="entry name" value="SRR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SRR1-like" evidence="2">
        <location>
            <begin position="44"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR22926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="264" mass="30702" checksum="0DCCE3117652D395" modified="2009-07-28" version="1">MTESLESGFRQVRRTKEPRGQVGFDVSLAKHRETVRSSEMFKNLKEHLEPHSKDIRRVRCLAIGSFHEEFAALYQLALLLETCDHLKPSEGEPIRVSVYDPVFTTSDQDFIKNLGPDWSVDEETPWEPSEARYVLFFLPHAPLDLTERVVTLDRPHLWLANHIVKHTDRYTKLQLFDKYPVLSKLLNSLVNAQESVPDSEFKPFVSRKNRRKNKTRIQEQVVDYSAIPTHFSKCKILTDFNNGKTLADQPWINSFSDLTLHLIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFL0</accession>
    <name>C5DFL0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0D15994p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0D15994g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553403.1">
        <property type="nucleotide sequence ID" value="XM_002553357.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFL0"/>
    <dbReference type="FunCoup" id="C5DFL0">
        <property type="interactions" value="64"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFL0"/>
    <dbReference type="GeneID" id="8295650"/>
    <dbReference type="KEGG" id="lth:KLTH0D15994g"/>
    <dbReference type="eggNOG" id="KOG2226">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003989_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFL0"/>
    <dbReference type="OMA" id="WENGINT"/>
    <dbReference type="OrthoDB" id="432953at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005797">
        <property type="term" value="C:Golgi medial cisterna"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000138">
        <property type="term" value="C:Golgi trans cisterna"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026705">
        <property type="entry name" value="Hid-1/Ecm30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21575">
        <property type="entry name" value="PROTEIN HID1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21575:SF12">
        <property type="entry name" value="PROTEIN HID1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12722">
        <property type="entry name" value="Hid1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="433"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="659"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="996"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1175"/>
            <end position="1204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="433"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="453"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1012"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1179"/>
            <end position="1204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1204" mass="135191" checksum="C1B82C6F6E3EAB31" modified="2009-07-28" version="1">MGQSDSKLTQYRDHIFQLADPGVLPLYSPSAHPRDIIRCYNDPLSQNGLTGEVEDGAGDALPDEELFSPVFSAFASSDAEARDVYSVVSTQELRLIFQSNQANFISLVRFFAFKIHALACQLQHCDDVDEFRARRAELLSSVRIMTKIMPVFFEMDTDQSLEDSLFWSRDSSQALQCGELPAPVVVPKLNVNHAPTESQAVVPLGVALLQACVKLLFMQGFTLPLSKPQLDELGKTSFLLWENGINTSESNYRPPSPKLDSNRLEVLRLLLTVSSKELYSNHIPKFLCVLTTSLPEFHIICLASSLLNLVCRSCRNNEENNGLNYPPNSYHTSCKSTNMRALRKALAAASAQMLNLSLLCSIEGPRRSEIYLFLYGLNLYSTNYEIKNLVSSYLGTLNREFDLKFILISLVTLLKRPIDRAIENESNPFSLLNGSSNASTKQGKGPTKKNGHSRSNSAKGASSKSNLSRLPRSTLQVTTLLWELMKCNKSFENYVADKYANKLLLVCIFHIKYYSDSPDWRTSLIPAISGFAIYLSSKKLVFSKMSFCFNVNYYTNKLPNFFKLSVGDVNHLTYRDFAIIHLSNIAMNQVHENLFLDPNLIEIIYNLLPMAHEKDGGELVQLSSERSNKLTGLSYNACVALLQLISKMTSKNYLTTFAGEKGDPDSSGNSSDATINKPDEKAKPYAFSPGFKLDTLALLLHAFNSCIFNYFRDSKHLLFVLCRHARVVQRLDEILQEISSEINYVYDRQDSRASLKPNSLFISDFFDDSLPYDVRISQANTGLQSFSGSEYSLMEKAQLQSLLFFDAEKTYSEEVEATAERIANGSPNKTIELFEHSDKEHNPILSSTELLLALRPIRPLGLSFKSKLKTRKEGPLKDSWLGSQTLSILKRTVKIVNKMFPEIPQVTTATYIPLLRKLLESESFLKKELSHAMPYETKISIKVTNPLLISWEHSAPASRWYLSILWADIFNYNSVPFISAYTKENSQQDAFLQHQNQSLSQPQTPRLERHNSQGSSLSRTNSNSSSISTFFLHQEPESASNSAPNSPSVGSSFSSWLNNKYQAGSQNNERSSLFRFSWAGFQKNPQDEKICEEISNEPEDDLVSPEGSFVLDMGLLKPNIWVGTRVELFKTKVDERENYSLMEMTSNFLRKLRLGNSWGVNAPDGVMVTPGTLAPGTARSWTPRNSMIGNGTASLQRHPNGSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5DG81</accession>
    <name>C5DG81_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0D03102p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0D03102g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552861.1">
        <property type="nucleotide sequence ID" value="XM_002552815.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DG81"/>
    <dbReference type="FunCoup" id="C5DG81">
        <property type="interactions" value="136"/>
    </dbReference>
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        <property type="taxonomic scope" value="Eukaryota"/>
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        <property type="component" value="Chromosome D"/>
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        <property type="term" value="C:Rpd3L-Expanded complex"/>
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        <property type="term" value="C:Set3 complex"/>
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    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15550">
        <property type="entry name" value="PHD_MLL5"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
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    </dbReference>
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        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
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        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
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        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
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    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46462">
        <property type="entry name" value="UPSET, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="UPSET, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20826">
        <property type="entry name" value="PHD_5"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="7">
        <location>
            <begin position="177"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="SET" evidence="8">
        <location>
            <begin position="343"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="23"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="130"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="230"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="640"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="68"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="136"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="230"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="255"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="650"/>
            <end position="659"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR22423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="751" mass="83421" checksum="A51E32E724628862" modified="2009-07-28" version="1">MGGNDISERSVYEDASALLMLSRGKDSSTNHTPLQDIKQPIGSPGLRSSSQVHSEPRSPLTSGDVRSRASSISLIQHNSTASPGPAYATLSAEDEDGLSSRMHEKTESPSSRSNSNKGIVAAAALAAAATVPLPLRKQDPEHRESKHNSRKSDHSEQRPKRSWPVPNSYVVDPDSGIITCICGYDDDDGFTIQCDHCNRWQHAICHGIRNIETAPDDYLCSNCHPRKLDVKRAKRKQQERLNPRNNKKRRKSSQGEESSGKSNSSHASDSANTSVNNLGGNEVASSAELKPTQRHLNSLEHHPVVYVPLTANDFKDRYVEMFVERHCDDDWVLPYSHRIFQPVPLEVKSCSESSRSFTGLPKLGLFAKQPCTVGSYIEEVVGEVDFTKKYYGDSRNNYRVCGTAKPKVFIHPHWPIYIDCRLSGNLTRFIRRGCQPNVELVSVHLNGDAPQVKFVLRALKDIGDGDELQIGWQWDLRHPIWHLIKGDNKSVDSLDDPDKFTLVHSVDTILSCADCACGAHNRDCYLLKVKKFSQSLIKSVKSKMNGRYKLNEVLQNAQNKNKKLQTPILSRLAHEAISNAARANELLVDFHAAKLKYLKEEGIPVSQLISRQPSLSDTENFDHAKPFKLYLVNKHFTTLRGGGEQENHRPSITTGTSNSLRYDESHITDLKSLPLPVELQLPRLSSMGEKALSIRENSNGDVAPELSTKAPVTPSVALPAVAVAAGPAINSRHTLKKKLSFADYKKKMKPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGD4</accession>
    <name>C5DGD4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein mL60</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0D04400g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Component of the mitochondrial large ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552914.1">
        <property type="nucleotide sequence ID" value="XM_002552868.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGD4"/>
    <dbReference type="FunCoup" id="C5DGD4">
        <property type="interactions" value="123"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGD4"/>
    <dbReference type="GeneID" id="8295140"/>
    <dbReference type="KEGG" id="lth:KLTH0D04400g"/>
    <dbReference type="eggNOG" id="ENOG502S81F">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_141719_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGD4"/>
    <dbReference type="OMA" id="KYTVFDR"/>
    <dbReference type="OrthoDB" id="2332379at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016340">
        <property type="entry name" value="Ribosomal_mL60"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28271">
        <property type="entry name" value="54S RIBOSOMAL PROTEIN L31, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28271:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09784">
        <property type="entry name" value="L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002216">
        <property type="entry name" value="MRPL31_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR002216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="131" mass="15386" checksum="DE6673B86FF5C803" modified="2009-07-28" version="1">MFGPFKSSNTLLGGLLWKIPWRMSRPQKQRQRHRLQDVDSVIKSLNLGLHAERSVQKGISYDEAINSSKQLKPGVKQLRLLNKPSLFPNEPQMSYKDKYTYFNKQAKGYRKGIHKLPKWTKITQRRNPAFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGE4</accession>
    <name>C5DGE4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mediator of RNA polymerase II transcription subunit 11</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Mediator complex subunit 11</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">MED11</name>
        <name evidence="5" type="ordered locus">KLTH0D04642g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the Mediator complex subunit 11 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552924.1">
        <property type="nucleotide sequence ID" value="XM_002552878.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGE4"/>
    <dbReference type="FunCoup" id="C5DGE4">
        <property type="interactions" value="141"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGE4"/>
    <dbReference type="GeneID" id="8295150"/>
    <dbReference type="KEGG" id="lth:KLTH0D04642g"/>
    <dbReference type="eggNOG" id="ENOG502S3YW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121031_1_0_1"/>
    <dbReference type="InParanoid" id="C5DGE4"/>
    <dbReference type="OMA" id="IMDDNIG"/>
    <dbReference type="OrthoDB" id="5418434at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019404">
        <property type="entry name" value="Mediator_Med11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10280">
        <property type="entry name" value="Med11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR22486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="111" mass="12794" checksum="7A6665525CE722E2" modified="2009-07-28" version="1">MPQPEFIQERLESLNAVDNQLLSTLLHASQAVGTIEELKRGNENMKSQFENHIRSFYGSLEEATVALRREIRHLDENVGTRLLPINVNKKAVGQDNEKMREQFEHLEHEAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGM8</accession>
    <name>C5DGM8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0D06600p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0D06600g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553008.1">
        <property type="nucleotide sequence ID" value="XM_002552962.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGM8"/>
    <dbReference type="FunCoup" id="C5DGM8">
        <property type="interactions" value="61"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGM8"/>
    <dbReference type="GeneID" id="8295239"/>
    <dbReference type="KEGG" id="lth:KLTH0D06600g"/>
    <dbReference type="eggNOG" id="KOG4812">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041193_2_0_1"/>
    <dbReference type="InParanoid" id="C5DGM8"/>
    <dbReference type="OMA" id="YNEICID"/>
    <dbReference type="OrthoDB" id="10003116at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030001">
        <property type="term" value="P:metal ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031398">
        <property type="term" value="P:positive regulation of protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22212">
        <property type="entry name" value="NDFIP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019325">
        <property type="entry name" value="NEDD4/Bsd2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13396:SF5">
        <property type="entry name" value="NEDD4 FAMILY INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13396">
        <property type="entry name" value="NEDD4 FAMILY INTERACTING PROTEIN 1/2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10176">
        <property type="entry name" value="NEDD4_Bsd2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="180"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="281"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR22570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="320" mass="35569" checksum="8D28B9ECB1D99590" modified="2009-07-28" version="1">MACKFRKRILSIVHCIQITKRSRYKHKAPACCKRSEHMAELNLGAGVVGRSSDAIPESDSHGQDAPQGQRGFASTLQENIMRSFDSVGKKFNVLDRLFKKNNGTTGLQFGANADGVFSNLTAKPDESGSVELQDRDKPPNYEEAAADMAPSYYGMDEDGIGMYYNEICFEGLPVGNVVNLLWNIIVSVSFQFVGFLLTYILHTSHAAKQGSRFGLGLTFLGYGYSMIPNDVTSKVGKSKSISKLKPLDPNEFDDMHLYSGSATKDEFESSLSHGIDEKKSGIPFLAILLFVSGFSIMFKSIYDYVKVKRKERRYLTQEQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGN0</accession>
    <name>C5DGN0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">KLTH0D06644p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0D06644g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553010.1">
        <property type="nucleotide sequence ID" value="XM_002552964.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGN0"/>
    <dbReference type="FunCoup" id="C5DGN0">
        <property type="interactions" value="237"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGN0"/>
    <dbReference type="GeneID" id="8295241"/>
    <dbReference type="KEGG" id="lth:KLTH0D06644g"/>
    <dbReference type="eggNOG" id="KOG0752">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015166_10_0_1"/>
    <dbReference type="InParanoid" id="C5DGN0"/>
    <dbReference type="OMA" id="VYERMKW"/>
    <dbReference type="OrthoDB" id="270584at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000151">
        <property type="entry name" value="LEU5p Mitochondrial carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002167">
        <property type="entry name" value="GDC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24089">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00928">
        <property type="entry name" value="GRAVESDC"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="315"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="25"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="124"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="256"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR22572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="349" mass="39536" checksum="B49C866FFF9F92BB" modified="2009-07-28" version="1">MCNADNNNEIRASPEARMVDKYSAHYILRSGLAGGIAGSCAKTLIAPLDRIKILFQTSNPHYLKYSGSMLGLVQAGFHINARDGIRGFYQGHSATLIRIFPYAAIKFIAYEQIRNVMIPSKEYETHARRLASGSLAGLCSVFMTYPLDLIRVRLAYVTDHRRIRMLPIIKQIYTERASESLTSKPFVPQWFAHWCNFYRGFTPTVLGMIPYAGVSFFAHDLCGDILRSSVLAPYSVLHVSNEELSMRSKKQNNRPLKTWAELVAGGLAGMASQTASYPFEIIRRRLQVSVVSPTSIHEFQSIPDMIRVIYKERGWRGFFVGLSIGYIKVTPMVACSFFVYERMKWYLAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGP6</accession>
    <name>C5DGP6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0D07018p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0D07018g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PDCD5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553026.1">
        <property type="nucleotide sequence ID" value="XM_002552980.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGP6"/>
    <dbReference type="FunCoup" id="C5DGP6">
        <property type="interactions" value="428"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGP6"/>
    <dbReference type="GeneID" id="8295257"/>
    <dbReference type="KEGG" id="lth:KLTH0D07018g"/>
    <dbReference type="eggNOG" id="KOG3431">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_122978_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGP6"/>
    <dbReference type="OMA" id="HANICTY"/>
    <dbReference type="OrthoDB" id="10252486at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.140">
        <property type="entry name" value="PDCD5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002836">
        <property type="entry name" value="PDCD5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036883">
        <property type="entry name" value="PDCD5-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10840">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10840:SF0">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01984">
        <property type="entry name" value="dsDNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015730">
        <property type="entry name" value="TFAR19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46950">
        <property type="entry name" value="Double-stranded DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="19"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR22588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="137" mass="14982" checksum="B3FFA044B8A9031D" modified="2009-07-28" version="1">MDPELQALREARLAELKNHANPAAAAAPGRPQKSAADADAVAALLQPQALERLSRVALVRPERARAVEAYLQQLAARGQLSRKVSEPEIVDILNGVARDEQRRSTTRIIFDRRDARDASIVTPNGAVTSDDDDDFFD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-03-08" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGU9</accession>
    <name>DEF1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName>RNA polymerase II degradation factor 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">DEF1</name>
        <name type="ordered locus">KLTH0D08470g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Recruits the ubiquitination machinery to RNA polymerase II for polyubiquitination, removal and degradation, when the transcription-coupled repair (TCR) factor RAD26 fails to efficiently displace stalled RNA polymerase II. Also involved in telomere length regulation. Binds DNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer; may form higher order oligomers. Interacts with the large RNA polymerase II subunit RPO21; the interaction is direct and serves to bridge RPO21 to the Elongin complex in a manner dependent on transcription stress. Interacts with RAD26.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
            <location evidence="1">Telomere</location>
        </subcellularLocation>
        <text evidence="1">During transcription stress, localizes to the nucleus following proteolytic cleavage by the proteasome.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">Ubiquitinated.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">Proteolytically cleaved by the proteasome in response to transcription stress; the resulting N-terminal form constitutes the activated nuclear form and the C-terminal portion is degraded.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DEF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553079.1">
        <property type="nucleotide sequence ID" value="XM_002553033.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGU9"/>
    <dbReference type="SMR" id="C5DGU9"/>
    <dbReference type="FunCoup" id="C5DGU9">
        <property type="interactions" value="305"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGU9"/>
    <dbReference type="GeneID" id="8295315"/>
    <dbReference type="KEGG" id="lth:KLTH0D08470g"/>
    <dbReference type="eggNOG" id="ENOG502S359">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023119_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGU9"/>
    <dbReference type="OMA" id="MYQNQFP"/>
    <dbReference type="OrthoDB" id="5396806at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14368">
        <property type="entry name" value="CUE_DEF1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003892">
        <property type="entry name" value="CUE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041803">
        <property type="entry name" value="DEF1_CUE"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51140">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0158">Chromosome</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0539">Nucleus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0779">Telomere</keyword>
    <keyword id="KW-0832">Ubl conjugation</keyword>
    <keyword id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="chain" id="PRO_0000405669" description="RNA polymerase II degradation factor 1">
        <location>
            <begin position="1"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="domain" description="CUE" evidence="2">
        <location>
            <begin position="24"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="61"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="366"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="518"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="633"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="68"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="104"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="158"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="180"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="206"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="243"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="279"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="318"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="382"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="424"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="457"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="542"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="580"/>
            <end position="590"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P35732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="733" mass="80081" checksum="6CE8FDB5078897F2" modified="2009-07-28" version="1">MSTQSRKSNSSKQPHSTHKKLDPELKFKLETLTELFPDWTNDDLIDLVQEYEDLETIIDKITSGAATKWDEVKKPSKKERQREQQQQQQQQQQAQLAAQQATQPSSQSHHNNHTHISPSHDGDHSHSAQTSHNHHQSKSSKFSSRERDSSSRSHKKSSNNAAASGPNGSGNARRERASGASRVPATSAASAASANASAVQPDHLKTAVSAAKTASSTSWAAMASDKKAAKQSAQAKKAEEQQQEQQSPQQAQHESTAPSPQQEAEPQSQSQSKSQPQSDNKSAESVSSTSTPATEDLEKPKKMTWAAIVKPKTKPSVKKSEPLEELEDLKREAAQISTEEPEAAEKVIEQVIEQVEQTPEEVVEQVEVTVVPEEVSQESEEASAQQEETAEPAAPVVPAEPEAASSEEKAAPAQPEQGTYSPVAQQQQPQQQQQPQQQQQAQQQQQQPTAYSEDKQSQAQQAQSQQAQSFYQAQPQQYGSQTPQQTPQTLQQQNAAAAAAAAQQQYYMYQNQFPGYSYPGMFDSQSYPAGYGQQYAPQSQNGSQPQTASTQQSQSGQYGVPPGYASTGRDLGAASPMAAQVQLQQQQQQQPYGGSFMPYYHFYQQSFPYGQPQYGMAGQYPYQVPKAYNYMNQYQPQQGGQTPSSQSQQGEEAQQSAQQGQATASQGQAQGSGSQGQAQTNAQQQAQLQQYYQFQQQQQQQQAAAAAAAAQQGGVPYGYSGYDFSSQATRGFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGZ3</accession>
    <name>C5DGZ3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0D09504p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0D09504g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553123.1">
        <property type="nucleotide sequence ID" value="XM_002553077.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGZ3"/>
    <dbReference type="STRING" id="559295.C5DGZ3"/>
    <dbReference type="GeneID" id="8295361"/>
    <dbReference type="KEGG" id="lth:KLTH0D09504g"/>
    <dbReference type="eggNOG" id="ENOG502QV33">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018393_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGZ3"/>
    <dbReference type="OMA" id="KSIMHIN"/>
    <dbReference type="OrthoDB" id="5367584at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024312">
        <property type="entry name" value="TACC_fungi"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12709">
        <property type="entry name" value="Fungal_TACC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="142"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="193"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="407"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="568"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="681"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="52"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="146"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR22685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="718" mass="82820" checksum="F4EFA2F4A415A78C" modified="2009-07-28" version="1">MGDSNIGSFSTPLKNGISRSVELRVPNSERPYPKGVDSPGKPANDDRALRIQKNNENASQLRSSPWKRAKLDKFSSRSHPVSSPAVIEMLDRNVSGPHGSLRDSQALEIQDEISEAISSAVREVSGSDSSNHDNQHRTSLINDLLSPRRDGTPDHIPDADLANLGKLTPPKNYMHNTESSIIQNLQSCFRDELGKFEQYLKAKNEQADEYRRRTVDCFEKIKQLEEALDEQKLTYSSLLGEYEMLRASQSVRDDQWQTQNREIEKLSTEKTSLEERVSRLKLRLSEARNEMKMINQNSQILQEKFQTQIQQNERLQNQVSELEDSENQLRVHIENTTNSKKRVEEEIERTRAELALLTEGKFKTQSENEDLKLSVSKAQTLLAEKTKFIEELQANLRASHDTKQKSTIELEQRINDLTLEKNELVRELEDMSTLRSKIEESENEIQSVKTELDRTQVALQHLKEEHSELREHCRSLEAELKTSHQKLETANDQLAIKSAEVVELGHDVKELRQGKSHLEDSLKLRDASISEWNSKYEEKCAENNKLSIEIESFQFKNGNLETEHLVELEQLHQQMTSLQETLKSNSERIKELTEENSSLKDQLEVRELPQPQQGPLVDPEQVEELHAKIQSLEQQLREKEAEAGKRLQLLAEDLYIQYSSKHEQKVKMLKKGYESKYQDKLERLGLENSGLKDELMQLNDTLKAEREEKHNLVKLLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHE0</accession>
    <name>C5DHE0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0E03608p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0E03608g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553638.1">
        <property type="nucleotide sequence ID" value="XM_002553592.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHE0"/>
    <dbReference type="FunCoup" id="C5DHE0">
        <property type="interactions" value="80"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHE0"/>
    <dbReference type="GeneID" id="8291787"/>
    <dbReference type="KEGG" id="lth:KLTH0E03608g"/>
    <dbReference type="eggNOG" id="ENOG502RXPE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073166_0_0_1"/>
    <dbReference type="InParanoid" id="C5DHE0"/>
    <dbReference type="OMA" id="FVENECK"/>
    <dbReference type="OrthoDB" id="198787at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021211">
        <property type="entry name" value="SAM35"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10806">
        <property type="entry name" value="SAM35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR23201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="324" mass="36801" checksum="72E7B4E08FA28DA8" modified="2009-07-28" version="1">MRSILQVPGPVKAFFDQFPLQTYPAVSKKDDASVYEYNAQSYGFREQEAAQRSANDVFKLGVYNVVEDPESGCVLATDPWCLYAQLSLCKKNGLKLAHKKGAQQQQQRQQQRQQPALLVVSRLAASDKSLPILIEGCSKRNVRSTAGINEILSSRLRDAEELMYVSLLDSVVYDCWTTQALCELSVAKFLELYDCAPIDVALLQRAFYQTLREDLLERNDFSLRHLEISRHVRYMHLYQSRNAHQLTAPLFESCKAALDRFAALLGSRTFFSGEMEPGYLDLKLASYVLCFLSLDDQESSLARYLRQEVHALAIHSRAVIAHFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHF5</accession>
    <name>C5DHF5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0E03938p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0E03938g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553653.1">
        <property type="nucleotide sequence ID" value="XM_002553607.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHF5"/>
    <dbReference type="FunCoup" id="C5DHF5">
        <property type="interactions" value="1087"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHF5"/>
    <dbReference type="GeneID" id="8291802"/>
    <dbReference type="KEGG" id="lth:KLTH0E03938g"/>
    <dbReference type="eggNOG" id="KOG2051">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002633_1_0_1"/>
    <dbReference type="InParanoid" id="C5DHF5"/>
    <dbReference type="OMA" id="DFQHHQI"/>
    <dbReference type="OrthoDB" id="27832at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035145">
        <property type="term" value="C:exon-exon junction complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003890">
        <property type="entry name" value="MIF4G-like_typ-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039762">
        <property type="entry name" value="Nmd2/UPF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007193">
        <property type="entry name" value="Upf2/Nmd2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12839">
        <property type="entry name" value="NONSENSE-MEDIATED MRNA DECAY PROTEIN 2 UP-FRAMESHIFT SUPPRESSOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12839:SF7">
        <property type="entry name" value="REGULATOR OF NONSENSE TRANSCRIPTS 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02854">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04050">
        <property type="entry name" value="Upf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00543">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIF4G" evidence="4">
        <location>
            <begin position="34"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="MIF4G" evidence="4">
        <location>
            <begin position="367"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="domain" description="MIF4G" evidence="4">
        <location>
            <begin position="572"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="801"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="801"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="821"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="881"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="901"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="930"/>
            <end position="949"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR23216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1068" mass="122717" checksum="4607D7713847F900" modified="2009-07-28" version="1">MNNERQLELFNLNSKAWAGESPLFEGGKKLDSSLKKNTAFIRKLKLGISKDSKQNFLKDIRELSLEKYLSELVSTSSEGLSKVSGKAEDVDAAVEVMSALHQRFSCKFTPVLMESFLNNFSLTQSSHESEKEELARILRLRSHVRIFTELYLVGLFRNTDYLNKENVPAFIAKKLSKKEPIIFTILKEVLNYRFRTGLTTTIATSFITKFPILFEGNAFNEELELESGTRKLLQSLIKVYTEACIGQAVELNKTLNKLKREHSKAQIRTGKNTDEYIEEYNDLLPVFERFEGAVNVLTDALQIEAPKLDKGIDSEANTTGPASIITNQLKPNSEKVWENEEVRKFYEVLPDLSSVHHDIEDRDAPDSTKLSDFFAGLEFADTREAIDELSVKYWSDSLNKKATRRRLLKFFIETQDWSKLRIYARFIASNAEYLSDTKDELIEYLDNGFRSQLRSNRINVKNIIFFSELVKFMLIPSYLVFHKIRTLAMNIQVPNNIEILTILFENFGKFLINHPTYKDQMEKMVDLIQQKRKDHDLTVSNKCALDNLIVLIYPPSLSKLNSAVKELNPEQQFYQIIIRRELHTISAEKTVKLLRKAHWKDEKIYKTMFSLFTKPEKVSYQSLDVLSSVLCGLYPYYRNFVIEVIDTLLEKIERGLEISEFNLNMVRMAQIKYLTSLFNKKLVRLEVLIEIFCKIMKFGYPGGRPNPYFLNEFDLPDNYFRVQLITTSLLSIEELPASKSDKMLLFMRLFEFYTFVKEQPLPRETQFKVSSAFQKLTSTDNFKRSCDVAESAKELANALNIPQATSLGSSPHQQTKKEGAYEEGEEDDDDDDEDDDAEIEEEVDNEEDDDEEDEDDDTEEGREPVKIIQEDEEGNLAALDSSDESASSDDSDDSGTSSNSDSDEEDGDDDEDDEDDEDDEDDDSIDLDGELERKRIHEEHLNRLRTEEELRAEEELEKQFQQLMQESMESRKNEKVSGNSIPIISAGYSTSLGKESGGEALRALNKSHDGKSKKVAFTFLTKSGKKTQAKTLGLPRNVKFVSGVLEEEQKLKAEREKIKNIVLNQKFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHN8</accession>
    <name>C5DHN8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Polyubiquitin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0E05852g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ubiquitin family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553736.1">
        <property type="nucleotide sequence ID" value="XM_002553690.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHN8"/>
    <dbReference type="FunCoup" id="C5DHN8">
        <property type="interactions" value="749"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHN8"/>
    <dbReference type="GeneID" id="8291889"/>
    <dbReference type="KEGG" id="lth:KLTH0E05852g"/>
    <dbReference type="eggNOG" id="KOG0001">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010412_0_1_1"/>
    <dbReference type="InParanoid" id="C5DHN8"/>
    <dbReference type="OMA" id="VHENTRR"/>
    <dbReference type="OrthoDB" id="428577at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031386">
        <property type="term" value="F:protein tag activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01803">
        <property type="entry name" value="Ubl_ubiquitin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000004">
        <property type="entry name" value="Polyubiquitin Ubiquitin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019954">
        <property type="entry name" value="Ubiquitin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019956">
        <property type="entry name" value="Ubiquitin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050158">
        <property type="entry name" value="Ubiquitin_ubiquitin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10666">
        <property type="entry name" value="UBIQUITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00348">
        <property type="entry name" value="UBIQUITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00299">
        <property type="entry name" value="UBIQUITIN_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="10">
        <location>
            <begin position="105"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="10">
        <location>
            <begin position="181"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="10">
        <location>
            <begin position="257"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="10">
        <location>
            <begin position="333"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="89"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00069010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR23299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="409" mass="46175" checksum="5E3D12C4DBE9FB2D" modified="2009-07-28" version="1">MVIPEHQLGAELPKPIFAVENYVREQCSSCGGRDKRKGCERGARFRGRLSEANGLPEHRSINTKELEYLPPLLTAERHSFPSVKASNNDTRKRLTERKTQARDTMQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHR0</accession>
    <name>C5DHR0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0E06380p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0E06380g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553758.1">
        <property type="nucleotide sequence ID" value="XM_002553712.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DHR0">
        <property type="interactions" value="339"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHR0"/>
    <dbReference type="GeneID" id="8291912"/>
    <dbReference type="KEGG" id="lth:KLTH0E06380g"/>
    <dbReference type="eggNOG" id="ENOG502QSQ9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_252311_0_0_1"/>
    <dbReference type="InParanoid" id="C5DHR0"/>
    <dbReference type="OMA" id="WQSHHKV"/>
    <dbReference type="OrthoDB" id="10001928at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056737">
        <property type="entry name" value="Beta-prop_ATRN-MKLN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43503">
        <property type="entry name" value="MCG48959-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43503:SF2">
        <property type="entry name" value="NEGATIVE REGULATOR OF SPORULATION MDS3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24981">
        <property type="entry name" value="Beta-prop_ATRN-LZTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Attractin/MKLN-like beta-propeller" evidence="4">
        <location>
            <begin position="131"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="560"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="621"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="668"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="716"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1124"/>
            <end position="1147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1177"/>
            <end position="1198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1240"/>
            <end position="1331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1423"/>
            <end position="1523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1547"/>
            <end position="1577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="564"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="677"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="720"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1131"/>
            <end position="1142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1240"/>
            <end position="1253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1286"/>
            <end position="1297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1452"/>
            <end position="1469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1480"/>
            <end position="1499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1508"/>
            <end position="1522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR23321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1577" mass="173225" checksum="1446772533F0161F" modified="2009-07-28" version="1">MSPLLPSPVTSHTLKLPELDPQSQVSLSECEKKARTLSCRTGSAVVLARSAIFVNGGFTIPLDLAEVNSISIQKELILYFSRESKSPDSFQNLSEWISTETFFLDLISRTWERVITEEGTSPIVEGSENNKSAVMRERVFHSICHHDGHLYIFGGLVVSSQSDYELIASNELWSLDLETKTWLRLSSDPSITRRFNHSMHLVPGNDESEDTRIYIVGGLNKFDRPVHVVDCFNLTQQRWEFADDEPDHGNRPEIITNINGQNTHLVKNSGFSLLLRDQISDEPSIAMYNPSDDGSGDIVNPFVAQPLVPGSRGQRMMSFQGYSSSEKQSFEVPFNLQHASGDYFGYNIILSGFHPDVQPENFRCFTYNIPTGKWTEINTMSDDPSGATPRLWQLFVWHSHHKVVFLGTRGKGNHLPSVQKFDTLLCIALPMINVFHKAMHYAQGTRGKSAASSEQLTLGHEGFEGYSRYSAPPLEITTIGSVFPSHAMALGKDSLEFYGQHLSDFEIVTEDGDSVRVPMYLLRKRWGRYFDEVLSQGYVKAIKDFENQNRHNEFAKLSYDSSHHNVSTPTTQHFGSTGSKDAPGTSLNGSFDAGNSKTEQVQVSQPLYPLVSRKSSPLFFANKSSQNDKAEKNDNGTADSRNAPAMHHSKSDSSAICSANTLSQYSKDAQDTVKADSQTSQNSNKTTSSSGGMVFRLPFQEASNTTFSTLVPHASMLDSGRSDGGDRHLGQYNRRRSSASTSLLFDVGGQNSRRASHPAVFNSDERFAIPGILQQSQSPFCSRKASVTSQNSSISYVSSTSDRMGNPAYRHMSQDSGTTNSTFNSVGSQIPPPQPMPMEPVPIPPQSHLEISNCPPSAKNSPFSSRRSSLYHELLRPGLASSVSQSIIGVQGNSGLESGPLYEPDPHSIHKRSMDRQLLEDNLIDVELEGNFGNVSQRYTATRCPKASREDSYGNTSVHVEGGRPSYFSTADSNCSVTGTITNELEPLLLPRSLYMPWPTSTIRSFTEFFYTGQVNGKWLLSPVALNLLVMAKLYEIPLLYDLMSEVFYSILGRKGESLLTTAEAMRHVFVSSFLAKHEGNEETVKQLKENFVYQEILDIEDSLRSIDNGHFNTHLLRDLTRLGSTSSSESGEKITSREGKDGTSLNVPILFAGGPRGSHNSVGSVGLPPNLALSPFKGAQPNISQPHKKSSLSKDVTADQPERVNLEGVNGTKTVTLERTEKTNQENKAEKIEQMKLSNMSQNESSALSNQVAGIKSPTGGCEGEAEKEPEYNMKQGENNKTLEDSTSSSESGDISAGFGLASTSKIEKKLRRRDSEKSIDPLSKLSDSRNSSLKNVIPYFKQGEKYFKTDNSPRNVDTLTLDNMASPNALPPVDYVIELIHETASLVHDVRLIVRCISVIKLSKALKSLKQQLDHEFASLSEEFRRAETPTDTTPRSPGPGLKSDGAPTLTPTTSVDKITKTTSNISLAKKISKPGTPESNARNPNTEVASRFTVSDPNLADKSDTASINTKNSKASSKQARIKKVNSHLAGSGLTGAALFMSGSFAPTPSKPKKESSSNNLSAMGGIPFFGKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHR5</accession>
    <name>C5DHR5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">Protein phosphatase PP2A regulatory subunit B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0E06534g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Phosphatase 2A affects a variety of biological processes in the cell such as transcription, cell cycle progression and cellular morphogenesis, and provides an initial identification of critical substrates for this phosphatase. The regulatory subunit may direct the catalytic subunit to distinct, albeit overlapping, subsets of substrates.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the phosphatase 2A regulatory subunit B family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553763.1">
        <property type="nucleotide sequence ID" value="XM_002553717.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHR5"/>
    <dbReference type="FunCoup" id="C5DHR5">
        <property type="interactions" value="894"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHR5"/>
    <dbReference type="GeneID" id="8291918"/>
    <dbReference type="KEGG" id="lth:KLTH0E06534g"/>
    <dbReference type="eggNOG" id="KOG1354">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021713_3_3_1"/>
    <dbReference type="InParanoid" id="C5DHR5"/>
    <dbReference type="OMA" id="NQIKWCR"/>
    <dbReference type="OrthoDB" id="6274823at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010972">
        <property type="term" value="P:negative regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902531">
        <property type="term" value="P:regulation of intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000189">
        <property type="entry name" value="Protein phosphatase PP2A regulatory subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000009">
        <property type="entry name" value="PP2A_PR55"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018067">
        <property type="entry name" value="PP2A_PR55_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11871">
        <property type="entry name" value="PROTEIN PHOSPHATASE PP2A REGULATORY SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037309">
        <property type="entry name" value="PP2A_PR55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00600">
        <property type="entry name" value="PP2APR55"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01024">
        <property type="entry name" value="PR55_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01025">
        <property type="entry name" value="PR55_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 7" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00055525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00067298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU331113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR23326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="460" mass="52068" checksum="11351FCB37E9CDA1" modified="2009-07-28" version="1">MSKNSFDFKFSQCFGDKADIVVTEADIITAVEFDHTGDFLATGDRGGRVVLFERNNSKHCEYKFLTEFQSHDAEFDYLKSLEIEEKINQIKWCRPTNQSHFLLSTNDKTIKLWKVYEKNIKLVSENNLSENSFARGSTGSGNGSINSSNGNGAAKAVLSLSTLKLPKLSQHDKIIAAAPKKIYSNAHTYHINSISINSDQETFISADDLRINLWNLDIPDQSFNIVDIKPANMEELTEVITSAEFHPQHCNLFMYSSSKGTIKLADMRQNALCDYRAKTFEEYMDPINHNFFTEITSSISDVKFSPDGRYIASRDYLTVKIWDINMGNKPVKTINIQDQLKDRLSDTYENDAIFDKFEVSFSGDSSSLMTGSYNNTFMIYPDVVKGKTSTSPPMDEIVLQADKTAFKSKKLGSLEQQSARNKEWGDDIDFKKSILHFSWHPRENSVAIAATNNLFIFSAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHY8</accession>
    <name>C5DHY8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0E08228p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0E08228g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553836.1">
        <property type="nucleotide sequence ID" value="XM_002553790.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHY8"/>
    <dbReference type="FunCoup" id="C5DHY8">
        <property type="interactions" value="311"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHY8"/>
    <dbReference type="GeneID" id="8291995"/>
    <dbReference type="KEGG" id="lth:KLTH0E08228g"/>
    <dbReference type="eggNOG" id="ENOG502RZ4Z">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_618299_0_0_1"/>
    <dbReference type="InParanoid" id="C5DHY8"/>
    <dbReference type="OMA" id="NDNEGKH"/>
    <dbReference type="OrthoDB" id="5370852at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044615">
        <property type="term" value="C:nuclear pore nuclear basket"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008298">
        <property type="term" value="P:intracellular mRNA localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031990">
        <property type="term" value="P:mRNA export from nucleus in response to heat stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006607">
        <property type="term" value="P:NLS-bearing protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034398">
        <property type="term" value="P:telomere tethering at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034432">
        <property type="entry name" value="Nup60"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28284">
        <property type="entry name" value="NUCLEOPORIN NUP60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28284:SF1">
        <property type="entry name" value="NUCLEOPORIN NUP60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="205"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="322"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="223"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="323"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="346"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="387"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="443" mass="47520" checksum="58E13549E21161C6" modified="2009-07-28" version="1">MNAKLEPNSNDNNLGDTEEAKLPNLTLAQFFREKGDEPLSEIEYEGVLSLIKRSKSASLVEDTQNALMAGNNSSELSSAVLKPSSVREASLKAPSFRPKYDEFASTANTSLRSVVSAGSRKSRVFDYSSLPSPYRSCSYRHSTTDAILAQKNTAGLESSGADAFSTSSDKMSNTASALISLLDSREAKSEASGLANPYSTRVSEFKRYRRDPESDRFVPHTPSKANGASSPAHSIALSEIQNNENKEAETTEKPTNQQFTKYRPAKASSLRTTVSAKKSPGAESSKPLGSVPHLQETDAPVVPKSSFGFSYPALAMSFDKSSRNANSANSTGKMAAGVSEEGTETKPASSMNTKTTPSFNFSQQLGARKANSPAQTSLAAFAKESDQSSARKANEGQSSRELQSAFSNNEPLQFDFSAPPASGIDPASIDEELVQKSRNIYVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DI02</accession>
    <name>C5DI02_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0E08602p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0E08602g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553850.1">
        <property type="nucleotide sequence ID" value="XM_002553804.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DI02"/>
    <dbReference type="FunCoup" id="C5DI02">
        <property type="interactions" value="76"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DI02"/>
    <dbReference type="GeneID" id="8292011"/>
    <dbReference type="KEGG" id="lth:KLTH0E08602g"/>
    <dbReference type="eggNOG" id="ENOG502QT80">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016397_0_0_1"/>
    <dbReference type="InParanoid" id="C5DI02"/>
    <dbReference type="OMA" id="PPFYILT"/>
    <dbReference type="OrthoDB" id="5586090at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006614">
        <property type="entry name" value="Peroxin/Ferlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052646">
        <property type="entry name" value="Peroxisomal_PEX28-32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010482">
        <property type="entry name" value="TECPR1-like_DysF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31679">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX30-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31679:SF2">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX30-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06398">
        <property type="entry name" value="Pex24p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00694">
        <property type="entry name" value="DysFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00693">
        <property type="entry name" value="DysFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="91"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="176"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Peroxin/Ferlin" evidence="8">
        <location>
            <begin position="273"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="Peroxin/Ferlin" evidence="9">
        <location>
            <begin position="364"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="35"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="413"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="464"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="413"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="478"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR23413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="522" mass="58936" checksum="7C6C0DFBF048EAEE" modified="2009-07-28" version="1">MSEHPKERETRARFVEEPTARLGGKTNKVLRSALKNRGKKALGESEQEEVESSPLLTSTPPTVSKALVRLYPYLIITDKVLSIITWTNDDIWPSVLVVVGYITSVLYFQNILKYFGHLVLVGILGCYSLLDMFVEESIKDRPTLDDIVHIITCVNAKADLLLSPIAVLTGNDVKRLLFTTIFLSPLYVIITLFIFPPRRLLLFGGVYLLTYHSAWSRVSRKLLWKFKLVRLLVFYITGLDLSGVNKNQGIFAAVQSKVKKMSSTRGISEEGKPIRFTYVLYENQRKWLGIGWTSNMLSYERAPWSDEFINEAPSPENFRLPEENMGMTWRWVDRTWRLDLTNDGAIQLPSSRPKTSAQPNSDEGFIYYDNTWKKPSTEDSFSKYTRRRRWIRTAELLRGDVLDVGGIMSSASAVDPTPQNVTVAGDTAKPEGTEPPSTILENVKELESFRRKVSFNDTEDVHIIPSDEKTRAPQLAVTDEDEENEAEGEASGITERSSARDLNVDPPPTSPPGAAVETKKHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>C5DI88</accession>
    <name>C5DI88_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 17">Homoaconitase, mitochondrial</fullName>
            <ecNumber evidence="5 17">4.2.1.36</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16 17">Homoaconitate hydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ordered locus">KLTH0E10582g</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 17">Catalyzes the reversible hydration of cis-homoaconitate to (2R,3S)-homoisocitrate, a step in the alpha-aminoadipate pathway for lysine biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 17">
            <text>(2R,3S)-homoisocitrate = cis-homoaconitate + H2O</text>
            <dbReference type="Rhea" id="RHEA:15485"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15404"/>
            <dbReference type="ChEBI" id="CHEBI:58174"/>
            <dbReference type="EC" id="4.2.1.36"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="17">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 17">Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-alpha-aminoadipate from 2-oxoglutarate: step 3/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 17">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">Belongs to the aconitase/IPM isomerase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.36" evidence="5 17"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553936.1">
        <property type="nucleotide sequence ID" value="XM_002553890.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DI88"/>
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        <property type="interactions" value="156"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DI88"/>
    <dbReference type="GeneID" id="8292101"/>
    <dbReference type="KEGG" id="lth:KLTH0E10582g"/>
    <dbReference type="eggNOG" id="KOG0453">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006714_3_1_1"/>
    <dbReference type="InParanoid" id="C5DI88"/>
    <dbReference type="OMA" id="LCDADNI"/>
    <dbReference type="OrthoDB" id="10262323at2759"/>
    <dbReference type="UniPathway" id="UPA00033">
        <property type="reaction ID" value="UER01027"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004409">
        <property type="term" value="F:homoaconitate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01582">
        <property type="entry name" value="Homoaconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01674">
        <property type="entry name" value="Homoaconitase_Swivel"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Homoaconitase, mitochondrial"/>
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    </dbReference>
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        <property type="entry name" value="Homoaconitase, mitochondrial"/>
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        <property type="entry name" value="Aconitase, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="entry name" value="Aconitase, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
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        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004418">
        <property type="entry name" value="Homoaconitase_mito"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039386">
        <property type="entry name" value="Homoaconitase_swivel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050067">
        <property type="entry name" value="IPM_dehydratase_rel_enz"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00139">
        <property type="entry name" value="h_aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43822:SF2">
        <property type="entry name" value="HOMOACONITASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43822">
        <property type="entry name" value="HOMOACONITASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="Aconitase iron-sulfur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52016">
        <property type="entry name" value="LeuD/IlvD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01244">
        <property type="entry name" value="ACONITASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 17" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10 17" id="KW-0408">Iron</keyword>
    <keyword evidence="11 17" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="14 17" id="KW-0456">Lyase</keyword>
    <keyword evidence="13 17" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="8 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 17" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 17" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha" evidence="19">
        <location>
            <begin position="22"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="Aconitase A/isopropylmalate dehydratase small subunit swivel" evidence="20">
        <location>
            <begin position="488"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="467"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00021560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00029338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00032706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU362038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="CAR23499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="687" mass="74241" checksum="B652E49A3B4FBC8A" modified="2009-07-28" version="1">MLRFARTFSSSRHVLRGQNMTEKIVQAYAVGLAEGKKVGSGDYVSIQPAHCMSHDNSWPVALKFMGLGASKIRNPRQIVNTLDHDVQNNTEKNLTKYKNIENFAKKHGVDFYPAGRGIGHQIMIEEGYAFPLNLTVASDSHSNTYGGIGCLGTPVVRTDAAAIWATGQTWWQIPPVAQVELKGELPHGISGKDIIVALCGIFNNDQVLNHAIEFTGDSLEKIPVDYRLTIANMTTEWGALSGLFPVDKTLINWYNNRLHKVGPNHPRINQQRIDDLVEKSKSLKADNDATYAKKLVIDLNTLTHYVSGPNSVKVSATVQDLSQQNIKVNKAYLVSCTNSRLSDLEAAAHVVCPTGDLNKVNKVAPGVEFFIAAASSEVERDAKASGAWEKLLRAGCKTLPAGCGPCIGLGTGLLEPGEVGISATNRNFKGRMGSKDALAYLASPSVVTASALLGKIGSPAEVLREQEPSFSGVKASVEDTQSQAKEGATASDKGPVEMLEGFPSVITGELVLCDADNINTDGIYPGKYTYQDDVTREKMAQVCMENYDAEFQTKVNAGDIVISGYNFGTGSSREQAATALLAKGIDLVVSGSFGNIFSRNSINNALLTLEIPALIEMLRAKYRDAPKELTRRTGWFLKWDLPNSTVIATEGSLDGPVVLEQKVGELGKNLQEIIVKGGLEGWVKSQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIM0</accession>
    <name>C5DIM0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0E13574p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0E13574g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the POMP/UMP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554068.1">
        <property type="nucleotide sequence ID" value="XM_002554022.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIM0"/>
    <dbReference type="FunCoup" id="C5DIM0">
        <property type="interactions" value="443"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIM0"/>
    <dbReference type="GeneID" id="8292237"/>
    <dbReference type="KEGG" id="lth:KLTH0E13574g"/>
    <dbReference type="eggNOG" id="KOG3061">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100687_0_1_1"/>
    <dbReference type="InParanoid" id="C5DIM0"/>
    <dbReference type="OMA" id="MSMRIVP"/>
    <dbReference type="OrthoDB" id="15001at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008012">
        <property type="entry name" value="Ump1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12828:SF3">
        <property type="entry name" value="PROTEASOME MATURATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12828">
        <property type="entry name" value="PROTEASOME MATURATION PROTEIN UMP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05348">
        <property type="entry name" value="UMP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00043974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR23631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="167" mass="18271" checksum="73823647049CA0ED" modified="2009-07-28" version="1">MLSSLNHRYCRSSQSIMSIIPSKSYQSSVSSISNSHAASNAAPALPDALRHQDGGSAPLSSQLNGKHPLEKSIQNWDQTQRERNMQQYKQVFGVAEPLRREMELQIVQNSDFNPLSSLTNGSSIHRDILLNKEASVDWEDIYPGTGLTGGVNLGADVHGSIEKQLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIS2</accession>
    <name>C5DIS2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Phenylalanine--tRNA ligase, mitochondrial</fullName>
            <ecNumber evidence="3">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Phenylalanyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">KLTH0E14740g</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Is responsible for the charging of tRNA(Phe) with phenylalanine in mitochondrial translation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>tRNA(Phe) + L-phenylalanine + ATP = L-phenylalanyl-tRNA(Phe) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="3"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554120.1">
        <property type="nucleotide sequence ID" value="XM_002554074.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIS2"/>
    <dbReference type="FunCoup" id="C5DIS2">
        <property type="interactions" value="795"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIS2"/>
    <dbReference type="GeneID" id="8292290"/>
    <dbReference type="KEGG" id="lth:KLTH0E14740g"/>
    <dbReference type="eggNOG" id="KOG2783">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022696_0_1_1"/>
    <dbReference type="InParanoid" id="C5DIS2"/>
    <dbReference type="OMA" id="PISHYPQ"/>
    <dbReference type="OrthoDB" id="4457at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00496">
        <property type="entry name" value="PheRS_alpha_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.380:FF:000002">
        <property type="entry name" value="phenylalanine--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000053">
        <property type="entry name" value="Phenylalanyl-tRNA synthetase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.380">
        <property type="entry name" value="Ferrodoxin-fold anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005121">
        <property type="entry name" value="Fdx_antiC-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036690">
        <property type="entry name" value="Fdx_antiC-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004530">
        <property type="entry name" value="Phe-tRNA-synth_IIc_mito"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002319">
        <property type="entry name" value="Phenylalanyl-tRNA_Synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00469">
        <property type="entry name" value="pheS_mito"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538:SF41">
        <property type="entry name" value="PHENYLALANINE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538">
        <property type="entry name" value="PHENYLALANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03147">
        <property type="entry name" value="FDX-ACB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01409">
        <property type="entry name" value="tRNA-synt_2d"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00896">
        <property type="entry name" value="FDX-ACB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54991">
        <property type="entry name" value="Anticodon-binding domain of PheRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51447">
        <property type="entry name" value="FDX_ACB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="15">
        <location>
            <begin position="158"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="FDX-ACB" evidence="16">
        <location>
            <begin position="375"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00057761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00073229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAR23683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="471" mass="54241" checksum="FDE9EE41B2E75503" modified="2009-07-28" version="1">MSRSRALISWCKTSLRRYSSAGSKITINGVEYETHPEFTNVSGTIHNLCDRSLHLKPSHPIGIIRKLIEDKLNSTSDAYSIYSNFKPVVTVEENFDSLGFPNDHPGRSKSDTYYVNDKHLFRTHTSAHEMECFQNIKAPRNVTGSGTRTSKPGFLISADVYRRDEIDRTHYPVFHQMEGAHIWTRSKETLKEGQEPTHIQKLRAELEAIEKQLQGESMKLQVEDDTSLEGNPKQDYMSDLEVELCSQHLKRSIELVVAQVFNKKVESMGGAAKDPVLPVRWVKAYFPWTAPSWEIEVFWQGEWLELCGSGIVREQVLLNAGYEHNSTIGWAFGLGLDRIAMLLFEIPDIRLFWTHDDRFHSQFEEGKISKFKPYSKYPGTTRDVAFWLPDDQTCKVIHENDLMEIVREMAGDLVESVKIIDDFTHPKTKKRSLCYRINYQSMDRNLTNDEINAIQESVGNELVDKFKVTLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-04-02" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIU9</accession>
    <name>C5DIU9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Pheromone-regulated membrane protein 10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0E15356g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ThrE exporter (TC 2.A.79) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554147.1">
        <property type="nucleotide sequence ID" value="XM_002554101.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIU9"/>
    <dbReference type="GeneID" id="8292318"/>
    <dbReference type="KEGG" id="lth:KLTH0E15356g"/>
    <dbReference type="eggNOG" id="ENOG502QPMM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007078_1_1_1"/>
    <dbReference type="InParanoid" id="C5DIU9"/>
    <dbReference type="OMA" id="MTLISCC"/>
    <dbReference type="OrthoDB" id="413008at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010619">
        <property type="entry name" value="ThrE-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051361">
        <property type="entry name" value="ThrE/Ser_Exporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024528">
        <property type="entry name" value="ThrE_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31082">
        <property type="entry name" value="PHEROMONE-REGULATED MEMBRANE PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31082:SF4">
        <property type="entry name" value="PHEROMONE-REGULATED MEMBRANE PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06738">
        <property type="entry name" value="ThrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12821">
        <property type="entry name" value="ThrE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="655"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="680"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="707"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="735"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="774"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="818"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="842"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="872"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="952"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="domain" description="Threonine/serine exporter-like N-terminal" evidence="9">
        <location>
            <begin position="551"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="domain" description="Threonine/Serine exporter ThrE" evidence="10">
        <location>
            <begin position="821"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="15"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="56"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="78"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="104"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="134"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="184"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="239"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="296"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF06738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF12821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR23710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="982" mass="106724" checksum="7E51383F5C179CCF" modified="2009-07-28" version="1">MSDTRPKMGARKSNRGSSERFQFLRPSSTARNRQQQHSPLAHSDSDSDSEDGAGTRGAPAASPRAGSPALPSFARPVAARDSRQRIIEQDAEDTIRVNSGNNLSDDDDDDDDGDQGAVQHYSHKDSSDSDSDGPENRKDHERREDGEDCGIERVAAPAARRRRSSGAVPPEANVGNQNYDGERSGSSSPEKSAKSSPSSAHRLSNPFNNNINNSIAEDEKDNDDGSSVSDEDKVDHFKRFFRRRSSTRRRSSHDSRPSGMRSVREEDEDAESGNGFLDKFLAYTGGGMTPGLTRAESQKSRPDRENVAGQENLEEPVEDSDIRETAEQILQTHGARFAEHGAQGHSGDAGNVGNAAAGAGAGEARNSFLEVGSPSDGETMVNSAQEHFFAPRVGHYDDIDTSEAFDPLMEDESYIPPPMQVRGGVLGSLLKMYQNEDDLASRSQVSLDTTPEAWDYYGEDAPLSGTMSGVTATEPGKKSKKISFGGLGKKTQSMLSSEHLPNGGKLPNFKATRPKMKNVKSVAKHANKLRRKQAAEAKITVHIAALLQRQRFILRLCKAFMLYGAPTHRLEEYMIMTSRVLEIDGQFLYVPGCMVVSFGDATTRTSEVQLVRCAQGLNLWKLHQVHAIYKSVIHDLISAEDANVAIDRIMAEPNLYPPWMCVLLYGFCSSMVTPFAFGGWWINMATSFFIGCCVGCLQFIVSQRSNLYSNVFEVTASIVVSFCGRALGSIKNSDICFGATVQGSLALILPGFIILTGSLELQSRNLVAGSVRMFYAIIYSLFLGFGITLGAALFGWIYNKGDRQASDETTCNRKVSPWYYFIFVPFFSIGLGLINQAKWSQLPVMTLISCCGYGVTYASGLHFKNSTEFRSAMAAFVIGILGNLYSRIWKGLAVSAMLPAIFVQVPSGIASQSTLLAGVQSANEITNTNGTTTVVSGTSDISSSMSFGVTMIQVSIGISVGLFASTIFVYPFGKKKTGLFTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIZ0</accession>
    <name>C5DIZ0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0E16280p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0E16280g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554188.1">
        <property type="nucleotide sequence ID" value="XM_002554142.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIZ0"/>
    <dbReference type="STRING" id="559295.C5DIZ0"/>
    <dbReference type="GeneID" id="8292360"/>
    <dbReference type="KEGG" id="lth:KLTH0E16280g"/>
    <dbReference type="eggNOG" id="KOG3113">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048955_2_1_1"/>
    <dbReference type="InParanoid" id="C5DIZ0"/>
    <dbReference type="OMA" id="YKSEEYT"/>
    <dbReference type="OrthoDB" id="247013at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006274">
        <property type="term" value="P:DNA replication termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006735">
        <property type="entry name" value="Rtf2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12775">
        <property type="entry name" value="PROTEIN C20ORF43 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12775:SF0">
        <property type="entry name" value="REPLICATION TERMINATION FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04641">
        <property type="entry name" value="Rtf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="182"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="182"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="214" mass="24155" checksum="74EC7C52B9AA6544" modified="2009-07-28" version="1">MGNDGSSIVRVKNLQVSLDHLPNKTRDKEAKNFDAVSRWTVCRLTGQPLRLPVVSDYLGNLLNKESILEWLLTPDKEDYSEEQIRLFGHIRSLKDVVELGNLVVDKDSRLKCDVGDEILGKSSSKLSYPAICGHVFPRRMLDNATEQPKCPVCDKPISKSDTITLNAESTDRDALEKRMIRLKRSNLTHSGKRSKSKKREAPAIGNSNTKRQRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJ11</accession>
    <name>C5DJ11_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0E16764p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0E16764g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tellurite-resistance/dicarboxylate transporter (TDT) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554209.1">
        <property type="nucleotide sequence ID" value="XM_002554163.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJ11"/>
    <dbReference type="FunCoup" id="C5DJ11">
        <property type="interactions" value="69"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJ11"/>
    <dbReference type="GeneID" id="8292382"/>
    <dbReference type="KEGG" id="lth:KLTH0E16764g"/>
    <dbReference type="eggNOG" id="ENOG502QT02">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030057_6_2_1"/>
    <dbReference type="InParanoid" id="C5DJ11"/>
    <dbReference type="OMA" id="AWHAVIM"/>
    <dbReference type="OrthoDB" id="1099at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000319">
        <property type="term" value="F:sulfite transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.150">
        <property type="entry name" value="Voltage-dependent anion channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004695">
        <property type="entry name" value="SLAC1/Mae1/Ssu1/TehA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051629">
        <property type="entry name" value="Sulfite_efflux_TDT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038665">
        <property type="entry name" value="Voltage-dep_anion_channel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31686">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31686:SF1">
        <property type="entry name" value="SULFITE EFFLUX PUMP SSU1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03595">
        <property type="entry name" value="SLAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="92"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="125"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="180"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="214"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="252"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="307"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="382"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR23772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="439" mass="49549" checksum="F125D8F199B6BEA0" modified="2009-07-28" version="1">MKSVFKGLEHLLVDEFSPFWFAQVVGTGICSTILHSYQMPWLLWCSYIMFSIASLLLILTLALMLTNMTRTVRKTGLRQYFAKYFDEPGNNVFWAFLPMGLGTLVNFLSQLCNHELKDSRRSRRLSIAVYSLWCLNTVISFATAWGVKFLLWRSREDKEVFNQDKLGQRNSGEALKPPHLLPIIPLLVVSSSSGLFTMSASFAHFADRKIQLSILIATSLIWLHAIFLAFFIICSCVWGFYINRFPPGKLAFTMFLIIGPLGQASFGILLLTDNIKRYIMLYLPITHKESVNAHIGNLIAAMSFKTLGFAAGLALISLGIFFTVMAFAAILSSSRKTPPDERKFKMVTFHKGWWAMPFPLGTMAVANKEFSRQYTPQIGGRVFEVIGIIYAVLCTTAVVTCMMGSIYIFCTAWKTTILEDQKNRLAKGVSINRPHELDV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2010-10-05" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJ44</accession>
    <name>AIM21_LACTC</name>
    <protein>
        <recommendedName>
            <fullName>Altered inheritance of mitochondria protein 21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">AIM21</name>
        <name type="ordered locus">KLTH0F13376g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in mitochondrial migration along actin filaments.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Actin patch</location>
        </subcellularLocation>
        <text evidence="1">Cortical actin patches.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the AIM21 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554770.1">
        <property type="nucleotide sequence ID" value="XM_002554724.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJ44"/>
    <dbReference type="FunCoup" id="C5DJ44">
        <property type="interactions" value="120"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJ44"/>
    <dbReference type="GeneID" id="8292994"/>
    <dbReference type="KEGG" id="lth:KLTH0F13376g"/>
    <dbReference type="eggNOG" id="ENOG502S25J">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_421542_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJ44"/>
    <dbReference type="OMA" id="VICERET"/>
    <dbReference type="OrthoDB" id="3995855at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021582">
        <property type="entry name" value="Aim21"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11489">
        <property type="entry name" value="Aim21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0206">Cytoskeleton</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000399522" description="Altered inheritance of mitochondria protein 21">
        <location>
            <begin position="1"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="197"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="559"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="63"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="85"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="198"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="242"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="264"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="282"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="320"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="368"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="407"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="444"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="488"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="515"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="562"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="578"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="640"/>
            <end position="650"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="650" mass="70179" checksum="13EED2902440809B" modified="2009-07-28" version="1">MQETQDQLEQMKTMISKPAGRKDYSLMQEESVPSTPSGSTVGSLITAVGQNEHNKVRNDEEATSSASPDGTPSTVDEGDVEESNSDISGVNEQNFENELETSLAEGKSWHSAQDLNKSHTEEPIDDPNEDKMSEINLEPSNKGIEKNATVTEKSATILAKELSATTDVKKKCLRAAKPNELPAEEYCNEDLKGAISGLERRTSKPDLETNEIQDAKSLESGIGSSGNVNEVPKQNAKHPMDSCQNLEQSAGTTSAEKEAIRALESQSSGTSQASLAKHKSETNTSIQSSTQETAQAPTVYEELGSEAERSKSHAQAYKNQTDEKEMSGEIIKENPENAIDDASSPSKEYDTPSFAGKPEEGSENLKAPAKDGEPVDSEKKKKNGGTPIEGRPLQSLEGDAPQVPQRPNKRPPPKKPSSKIAAFQAMLKQQQLMDASKTKDKQIDSGNSLLSGKRANITNNLNGIFGLPGMAPGMTPGMVPPQRPPARQQTGSDGGSQSSENQTSSQSAAPSGPQRRARGPKGRKLPAHIANVEKVETSIKTNEIQVLKTWSLKFQKISPTCSEKDSEPLKDIPESGVVSPSNPKSMSELNGVPVESDVICERETDILQNSKNEMNDLEDTNEDNVKEPRQFSIPTHFDLGESDANQEESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJ62</accession>
    <name>C5DJ62_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0F13816p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0F13816g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554788.1">
        <property type="nucleotide sequence ID" value="XM_002554742.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJ62"/>
    <dbReference type="FunCoup" id="C5DJ62">
        <property type="interactions" value="188"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJ62"/>
    <dbReference type="GeneID" id="8293014"/>
    <dbReference type="KEGG" id="lth:KLTH0F13816g"/>
    <dbReference type="eggNOG" id="KOG4206">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041869_3_2_1"/>
    <dbReference type="InParanoid" id="C5DJ62"/>
    <dbReference type="OMA" id="HIVFYDA"/>
    <dbReference type="OrthoDB" id="277802at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12246">
        <property type="entry name" value="RRM1_U1A_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="35"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR24351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="124" mass="14037" checksum="CF9A57112A30983E" modified="2009-07-28" version="1">MAVKKRSSEHTAAPLETKKPRAPSSTAEETISPKKTLYVNNLNDKIKVQTLRENLYLLFSTYGEVLQINVSNKNRGQAFIVLRTPDEANVAMISLQKETFFEKELHIQFSRQDSKLVASDSPAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJ81</accession>
    <name>C5DJ81_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">KLTH0F14234p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0F14234g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the Tom5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554807.1">
        <property type="nucleotide sequence ID" value="XM_002554761.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJ81"/>
    <dbReference type="FunCoup" id="C5DJ81">
        <property type="interactions" value="36"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJ81"/>
    <dbReference type="GeneID" id="8293033"/>
    <dbReference type="KEGG" id="lth:KLTH0F14234g"/>
    <dbReference type="eggNOG" id="ENOG502SD3F">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_209440_1_1_1"/>
    <dbReference type="InParanoid" id="C5DJ81"/>
    <dbReference type="OMA" id="SPIVWHF"/>
    <dbReference type="OrthoDB" id="3999796at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006626">
        <property type="term" value="P:protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019603">
        <property type="entry name" value="Tom5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10642">
        <property type="entry name" value="Tom5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR24370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="50" mass="5889" checksum="CE3837953A6870A0" modified="2009-07-28" version="1">MFGLPQQPPSEEEKKIHEQQSNQTLINAGYAAVLLWVSPIVWHFVKRQWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJB4</accession>
    <name>C5DJB4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Isocitrate dehydrogenase [NAD] subunit 2, mitochondrial</fullName>
            <ecNumber evidence="6">1.1.1.41</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Isocitric dehydrogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">NAD(+)-specific ICDH</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">KLTH0F15048g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-threo-isocitrate + NAD(+) = 2-oxoglutarate + CO2 + NADH</text>
            <dbReference type="Rhea" id="RHEA:23632"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.41"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Octamer of two non-identical subunits IDH1 and IDH2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.41" evidence="6"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554840.1">
        <property type="nucleotide sequence ID" value="XM_002554794.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJB4"/>
    <dbReference type="FunCoup" id="C5DJB4">
        <property type="interactions" value="963"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJB4"/>
    <dbReference type="GeneID" id="8293069"/>
    <dbReference type="KEGG" id="lth:KLTH0F15048g"/>
    <dbReference type="eggNOG" id="KOG0785">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031953_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJB4"/>
    <dbReference type="OMA" id="VRPCRYY"/>
    <dbReference type="OrthoDB" id="10261637at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004449">
        <property type="term" value="F:isocitrate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006102">
        <property type="term" value="P:isocitrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.718.10:FF:000003">
        <property type="entry name" value="Isocitrate dehydrogenase [NAD] subunit, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.718.10">
        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004434">
        <property type="entry name" value="Isocitrate_DH_NAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00175">
        <property type="entry name" value="mito_nad_idh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835">
        <property type="entry name" value="DECARBOXYLATING DEHYDROGENASES-ISOCITRATE, ISOPROPYLMALATE, TARTRATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835:SF34">
        <property type="entry name" value="ISOCITRATE DEHYDROGENASE [NAD] SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="15">
        <location>
            <begin position="37"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00072026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAR24403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="368" mass="39498" checksum="759941D4F6B7DEAD" modified="2009-07-28" version="1">MFKSSFVRNSARFLATKKQPSIGRYTGKPDPATGKYTVSFIEGDGVGPEISKAVKDIFAAAKAPIQWESCDVSPLFINGLTTIPQPAQDSINKNLIALKGPLATPIGKGHRSLNLTLRKTFGLFANVRPAKSVQGYKTAYDNVDLVLIRENTEGEYSGIEHVVSPGVVQSIKLITQDASERVIRYAFEYARAVERPKVVVVHKSTIQRLADGLFVSVAEQLSKEYPDIELQTELIDNTVLHTVRDPTKYSDVVVVCPNLYGDILSDMNSGLSAGSLGLTPSANIGHKVSIFEAVHGSAPDIAGQNKANPTALLLSSVMMLNHMGLTEHADKIEKAVFTTIAAGPESRTGDLGGSASTSSFAEAVISRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJC7</accession>
    <name>C5DJC7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0F15378p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0F15378g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the large ribosomal subunit (LSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes). The 5 acidic ribosomal P-proteins form the stalk structure of the 60S subunit. They are organized as a pentameric complex in which uL10/P0 interacts with 2 heterodimers, P1A-P2B and P1B-P2A.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic ribosomal protein P1/P2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554853.1">
        <property type="nucleotide sequence ID" value="XM_002554807.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJC7"/>
    <dbReference type="FunCoup" id="C5DJC7">
        <property type="interactions" value="1054"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJC7"/>
    <dbReference type="GeneID" id="8293084"/>
    <dbReference type="KEGG" id="lth:KLTH0F15378g"/>
    <dbReference type="eggNOG" id="KOG3449">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114656_0_1_1"/>
    <dbReference type="InParanoid" id="C5DJC7"/>
    <dbReference type="OMA" id="MKVIASY"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002182">
        <property type="term" value="P:cytoplasmic translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05833">
        <property type="entry name" value="Ribosomal_P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1410:FF:000002">
        <property type="entry name" value="60S acidic ribosomal protein P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01478">
        <property type="entry name" value="Ribosomal_L12_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038716">
        <property type="entry name" value="P1/P2_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027534">
        <property type="entry name" value="Ribosomal_P1/P2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001859">
        <property type="entry name" value="Ribosomal_P1/P2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044076">
        <property type="entry name" value="Ribosomal_P2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21141">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21141:SF5">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00428">
        <property type="entry name" value="Ribosomal_60s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00456">
        <property type="entry name" value="RIBOSOMALP2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950396" evidence="8">
        <location>
            <begin position="17"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="82"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="88"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00063629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR24416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="110" mass="10859" checksum="C5D5ADBA362A0777" modified="2009-07-28" version="1">MKYLAAYLLLVQGGNASPSAADIKKVVEAVGVEAEDAKISALLSSFEGKGSIDEIIAEGNTKLATVPAGGAAAAAGGAAAAGGAAPEAAEEKEEEAKEESDDDMGFGLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJI0</accession>
    <name>C5DJI0_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Endosomal/vacuolar adapter protein YPT35</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">PX domain-containing protein YPT35</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0F16588g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Recruits the lipid transfer protein VPS13 to endosomal and vacuolar membranes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endosome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the YPT35 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554906.1">
        <property type="nucleotide sequence ID" value="XM_002554860.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJI0"/>
    <dbReference type="FunCoup" id="C5DJI0">
        <property type="interactions" value="107"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJI0"/>
    <dbReference type="GeneID" id="8293139"/>
    <dbReference type="KEGG" id="lth:KLTH0F16588g"/>
    <dbReference type="eggNOG" id="ENOG502S40T">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070610_0_2_1"/>
    <dbReference type="InParanoid" id="C5DJI0"/>
    <dbReference type="OMA" id="FAVWKIT"/>
    <dbReference type="OrthoDB" id="10254720at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07280">
        <property type="entry name" value="PX_YPT35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037917">
        <property type="entry name" value="Ypt35_PX"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0967">Endosome</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0926">Vacuole</keyword>
    <feature type="domain" description="PX" evidence="11">
        <location>
            <begin position="86"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="31"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="44"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00033728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR24469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="214" mass="24347" checksum="BFB9215054DBF996" modified="2009-07-28" version="1">MPSVFQNSRLESCHLQNNVGLSIRSSLTEVHLSHQGPLRTSTGRQRRPRPSRTRKMPQSIQFLAPEPINLVNNEPSADEVTGSSLHFKNITVGSCTIVNSQNNKFSVWQLEVTLSPTGTSGAGSPHIRVYKRYTDFELFREKLLRSLTPALRPCVPQLPPKVSWYESWHYGQANFKSSWLARRRAGLEYFLNQVLLNDKVLTEALPCVKEFLEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJP6</accession>
    <name>C5DJP6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0F18106p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0F18106g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554972.1">
        <property type="nucleotide sequence ID" value="XM_002554926.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJP6"/>
    <dbReference type="FunCoup" id="C5DJP6">
        <property type="interactions" value="281"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJP6"/>
    <dbReference type="GeneID" id="8293207"/>
    <dbReference type="KEGG" id="lth:KLTH0F18106g"/>
    <dbReference type="eggNOG" id="ENOG502S51X">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077704_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJP6"/>
    <dbReference type="OMA" id="IRSKDRW"/>
    <dbReference type="OrthoDB" id="4096107at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013268">
        <property type="entry name" value="UTP16"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08297">
        <property type="entry name" value="U3_snoRNA_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="185"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="201"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="221"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="231" mass="26662" checksum="624436459BE08657" modified="2009-07-28" version="1">MVTPQKHKKFDDQEEALKDVPSEIPEKTSKVAEQDSDASSSDDDEAPEEEGMAQARSAVQQRDHERQQAAAREQEALREKRRKQDLRFKQQQEQKREREEKELEARLKAQVSSRGAAESGSIQDDDMVPAELPENFFEELEEQESATITTKPQHVNFNDIDDNYSQEIKQQLQKQKKKTLRQLRKTSMKRGPVKVNLLPPASNSKALAPQQDTTVTNKREKWLKRKALARK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJP9</accession>
    <name>C5DJP9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Patatin-like phospholipase domain-containing protein</fullName>
            <ecNumber evidence="5">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0F18172g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Lipid hydrolase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Membrane</location>
            <topology evidence="5">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PLPL family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="5"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554975.1">
        <property type="nucleotide sequence ID" value="XM_002554929.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJP9"/>
    <dbReference type="FunCoup" id="C5DJP9">
        <property type="interactions" value="113"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJP9"/>
    <dbReference type="GeneID" id="8293210"/>
    <dbReference type="KEGG" id="lth:KLTH0F18172g"/>
    <dbReference type="eggNOG" id="KOG2214">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009031_4_0_1"/>
    <dbReference type="InParanoid" id="C5DJP9"/>
    <dbReference type="OMA" id="FFTESDN"/>
    <dbReference type="OrthoDB" id="10049244at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006641">
        <property type="term" value="P:triglyceride metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07230">
        <property type="entry name" value="Pat_TGL4-5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1090.10:FF:000036">
        <property type="entry name" value="Patatin-like phospholipase domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050301">
        <property type="entry name" value="NTE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021771">
        <property type="entry name" value="Triacylglycerol_lipase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226">
        <property type="entry name" value="NEUROPATHY TARGET ESTERASE/SWISS CHEESE D.MELANOGASTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226:SF10">
        <property type="entry name" value="TRIACYLGLYCEROL LIPASE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11815">
        <property type="entry name" value="DUF3336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 4 5" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3 4 5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNPLA" evidence="7">
        <location>
            <begin position="224"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="652"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="733"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXGXXG" evidence="4">
        <location>
            <begin position="228"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="4">
        <location>
            <begin position="255"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="41"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="671"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="733"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="786"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="798"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="810"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="831"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="257"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR24538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="849" mass="95226" checksum="7256D6FB72A8E606" modified="2009-07-28" version="1">MVKSEFSLSGERLDITQRLIHRYYNGNLSKGNVSRRSGSAKKHDVEAPKTDADEIANEQGHSGVLSNLVSALKRVFSRDHERNVLIEKLTQEKQHATSYEEWYTTALQLDELTHTNDWKARDETTLYDYMLIKTHTKNMRDAREKRDYAQLLYLIRTTWVRNLGNMGNVNLYRHSHVGTKNLIDEYMRESKLALQELVRNSDLDENYLLGMLVQTRKNIGRTALVLSGGGTFGLFHIGVLATLFEQDLLPRVISGSSAGAIVASILCSCPKQDLVELVESVVGKEFNIFQDDSEKTQSENLLIKVSRFFKSGTWFDNKNLVKTMIGFLGDLTFREAYNKTGKILNITVSPATVFEQPGLLNNLTAPNVLVWSAVCASCSLPGIFPSTPIYEKDPKTGETREWNSSTVKFVDGSVDNDLPISRLSEMFNVDHIIACQVNIHVFPFLKMSVSCVGGEIEDEFSARFRQNLSSVYNFMANEVIHILEIATELGVATNVLTKFRSILSQQYSGDITILPEMNMLLRINELLANPSPEFLLRETVNGARATWPKISIIKNHCEQEFELDKAISFLKGRIISSPSFRTKNPFQFTDLSISLINTPEMISNDGHVPNVLDDTILNPNATDRLLVVNDTSRRPHSLGKTSISIAEASKQLHHYRRKSDSYAKGRGHSRYSISHPGNTSSHISSKLLPTLMGYSPRLDRKKSVGQIPFGSFGGANNEKQKEADLTLENQRHSNSLTTLHGSKNPSYYASNHTSPPSPLSAPISSALEYPTRKDSVSSYDSPLKSYYKRNSKQHRNRSPTVSRTSTGNPSKGKTSSRSKSTTKQEFKNPEYVDDSIDPVDSEDKGETEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJT5</accession>
    <name>C5DJT5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0F18986p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0F18986g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the KRI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555011.1">
        <property type="nucleotide sequence ID" value="XM_002554965.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJT5"/>
    <dbReference type="FunCoup" id="C5DJT5">
        <property type="interactions" value="711"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJT5"/>
    <dbReference type="GeneID" id="8293247"/>
    <dbReference type="KEGG" id="lth:KLTH0F18986g"/>
    <dbReference type="eggNOG" id="KOG2409">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009647_3_0_1"/>
    <dbReference type="InParanoid" id="C5DJT5"/>
    <dbReference type="OMA" id="KIMATRH"/>
    <dbReference type="OrthoDB" id="10252032at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018034">
        <property type="entry name" value="Kri1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024626">
        <property type="entry name" value="Kri1-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14490:SF5">
        <property type="entry name" value="PROTEIN KRI1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14490">
        <property type="entry name" value="ZINC FINGER, ZZ TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05178">
        <property type="entry name" value="Kri1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12936">
        <property type="entry name" value="Kri1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kri1-like C-terminal" evidence="3">
        <location>
            <begin position="453"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="133"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="272"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="367"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="516"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="290"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="395"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="516"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="535"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR24574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="562" mass="64792" checksum="CB867928B600860C" modified="2009-07-28" version="1">MPRKKSAAKRARESIQKEATKENTSVSEPKSRGPSKDNQNSDTSPKDLSEAEDESSSSEEEDDYGELVTEEVESGINQVLEAIKNNDKKLFDPSVRFFEDPKSAAAKLAKARKEKPVYLKDYHRMNILSGDALKDEDDEEMQTVDGKQSFVAQQKEEKDQLLEEIQDQFAEKEGSGDDSEDEGDFLVKKAPSKAQKVSDTSLPNPEVDDEKFLEAFVSKHAWIPKEGDREVNLDGPGMEEDDEEFDNAAEQFENAYNFRYEDPNAAEIVSYARNQATLRRSTSNSRRRKRDEEKREKTAEKMEKEMAVQKKKKEKVNKLTDVLEQVRKEYGAEIDEATVQKLTSTLLNGDFKDDQWDAVVSELFNEEYYNQEGKPTWDEDDEIMGDLYREGNKEEDADEDNNEEGETEEGPARKKSRRELKEEKRSKKKDKKKVSEMVETALEGNKLALVDEVEEERKSRARTKEEEDVKFRYREVSPESFGLTNREIFEADDGALNDFIGLKKFAPYRAKELRAKDRRKVTKSRRMREWKKKVFGSEEGLKDDASSQTQSQEKKHSKKAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJX1</accession>
    <name>C5DJX1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0F19778p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0F19778g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Peroxisome membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555047.1">
        <property type="nucleotide sequence ID" value="XM_002555001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJX1"/>
    <dbReference type="FunCoup" id="C5DJX1">
        <property type="interactions" value="286"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJX1"/>
    <dbReference type="GeneID" id="8293283"/>
    <dbReference type="KEGG" id="lth:KLTH0F19778g"/>
    <dbReference type="eggNOG" id="KOG4186">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049216_0_1_1"/>
    <dbReference type="InParanoid" id="C5DJX1"/>
    <dbReference type="OMA" id="IYRITQY"/>
    <dbReference type="OrthoDB" id="411017at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016559">
        <property type="term" value="P:peroxisome fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008733">
        <property type="entry name" value="PEX11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12652:SF50">
        <property type="entry name" value="PEROXIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12652">
        <property type="entry name" value="PEROXISOMAL BIOGENESIS FACTOR 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05648">
        <property type="entry name" value="PEX11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="1" id="KW-0962">Peroxisome biogenesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00046271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR24610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="221" mass="24935" checksum="D254476A57B15016" modified="2009-07-28" version="1">MVCDLLVYHPSVSRLIRLLDASAGREKVLRLLQYLCRFLGEQYSMVLARKLQAEFALVRKMLRFLKPLNHLQAASKFFDNKIASDNVVRWCNVIKNLAYAGYLALDQVTLLRLLRLVPNTPITGTRIPRWSNWCWFAGLSAGLVLDLRQLQVAREQGRGDKEATKAVAADRAKALRRLLWDSLDTFIVLNNLKFLHSPDNSVGLAGVATSLFGLQGLWEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5DK81</accession>
    <name>C5DK81_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0F02486p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0F02486g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IST1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554319.1">
        <property type="nucleotide sequence ID" value="XM_002554273.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DK81"/>
    <dbReference type="FunCoup" id="C5DK81">
        <property type="interactions" value="820"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DK81"/>
    <dbReference type="GeneID" id="8292511"/>
    <dbReference type="KEGG" id="lth:KLTH0F02486g"/>
    <dbReference type="eggNOG" id="KOG2027">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037652_2_0_1"/>
    <dbReference type="InParanoid" id="C5DK81"/>
    <dbReference type="OMA" id="HEVREFT"/>
    <dbReference type="OrthoDB" id="29853at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1260.60:FF:000002">
        <property type="entry name" value="Vacuolar protein sorting-associated protein IST1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.60">
        <property type="entry name" value="Vacuolar protein sorting-associated protein Ist1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005061">
        <property type="entry name" value="Ist1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042277">
        <property type="entry name" value="IST1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12161">
        <property type="entry name" value="IST1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12161:SF5">
        <property type="entry name" value="IST1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03398">
        <property type="entry name" value="Ist1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="172"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="172"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="229"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR23882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="264" mass="30061" checksum="D737F593EA5F0A5E" modified="2009-07-28" version="1">MCIQRLRYAQEKQQSLAKKARRDVAQLLADGKEQKAHYRIESLINDDVHEELLEVLELYCELLHARIALLCSVQDEADLIENHAENGLNEAARAVVFATLHAPEIKELQQAKELLTLKFGNDFTRTIIDEKLGVPDKVLKKCSPRLPDEELITLYLKEIAITYEVPFSKLEEASREEAEHEKSPTPTDSSSEDSRPIVAVDNNSLEDGKHAITVKKPRKDSDTMGSDLKVPRDMKKDVKVKNNKQNAHDTDLDELKKRFAALRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKG7</accession>
    <name>C5DKG7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein LST4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0F04532g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LST4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554405.1">
        <property type="nucleotide sequence ID" value="XM_002554359.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKG7"/>
    <dbReference type="FunCoup" id="C5DKG7">
        <property type="interactions" value="64"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKG7"/>
    <dbReference type="GeneID" id="8292600"/>
    <dbReference type="KEGG" id="lth:KLTH0F04532g"/>
    <dbReference type="eggNOG" id="ENOG502QPJF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010482_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKG7"/>
    <dbReference type="OMA" id="SEYDEYP"/>
    <dbReference type="OrthoDB" id="4063558at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037545">
        <property type="entry name" value="DENN_FNIP1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041153">
        <property type="entry name" value="LST4_longin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18639">
        <property type="entry name" value="Longin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51836">
        <property type="entry name" value="DENN_FNIP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="UDENN FNIP1/2-type" evidence="7">
        <location>
            <begin position="48"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="304"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="312"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR23968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="668" mass="75436" checksum="0F1EA0797611DB3A" modified="2009-07-28" version="1">MLGRLLAKNMLYESSSTADETSVPYVPHVSDETKQKLYGTKHLEMQASSAHRFRVVVAQELGQMMSRHNYQVVLDYATSRGSGMNHISVSDLKEYIFGSPVRAKTRHKCDKFRVVAGAGVVLVTRVFYPSQRDTRLAICLSLPESFLSALSEIWTHIGRWLDECQALLLAILSNDSLVSLPRDLRAHDPQITESIIQSIHTLIVPLVSSHTDIPRLLLYPSSSRQFVLSWFKDVFHWLDIKDGHRLRFLPVLFAKIAQDFAAELRANECSRIIIISGNMVVANKLIFLISALLGPRYSGRIDISDDTPQLGHSERQKPLKDERQSLDNKYFSTITGKGWEIPNKKTPNSSTSLSSDETSAQVIQPSSIRSSSSSLQYLSSSLSSVYGSYGSWFSKRIGMQSPSAKTNESSEQSYFQHHNGSSTSLHQVALNHNRVTPLPSPSINEYEEFPWCSSPSSPRTDNTQQGWPAHTEPSFQNVDIKRTCNRITNAKDLDQAFESICQFDSDSFKAETSAPSEGHAQVLEVELEIDSHSSYSELLPKYTSFLPNFVPWFQLQAVPVTSDAERKVLNAMKADLHTREYSRTLLISLRSREIKDIVVEKDHFLTKNVLRTKRVFSSGKCSSMSPKFQQQVLQCEQNIKKAMSVWEDVENVADKMEILQKIFEDIRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKI5</accession>
    <name>C5DKI5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0F04972p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0F04972g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554423.1">
        <property type="nucleotide sequence ID" value="XM_002554377.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKI5"/>
    <dbReference type="FunCoup" id="C5DKI5">
        <property type="interactions" value="100"/>
    </dbReference>
    <dbReference type="GeneID" id="8292620"/>
    <dbReference type="KEGG" id="lth:KLTH0F04972g"/>
    <dbReference type="eggNOG" id="ENOG502S82Z">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1555531_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKI5"/>
    <dbReference type="OMA" id="QLANMAH"/>
    <dbReference type="OrthoDB" id="4053693at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="51"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="117"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="144"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR23986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="172" mass="19017" checksum="6F6D67B0D6438154" modified="2009-07-28" version="1">MKPSDSGSHVDGDSRPKTGLHSENLSRETSGNSRVKAKPQYVPFKASKSNVFSNVVINSLFGLGLYYLYRDYQEDKRASAKARGTVTIVHDRASDSQAKGGQVRGSGLLPKMSYKDLSFVTVGFGFLLQLANMAHVSFGKRSPLYRASILSVALYPPFAYYMYSMRDQKVAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKL0</accession>
    <name>C5DKL0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0F05588p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0F05588g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554448.1">
        <property type="nucleotide sequence ID" value="XM_002554402.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKL0"/>
    <dbReference type="FunCoup" id="C5DKL0">
        <property type="interactions" value="408"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKL0"/>
    <dbReference type="GeneID" id="8292646"/>
    <dbReference type="KEGG" id="lth:KLTH0F05588g"/>
    <dbReference type="eggNOG" id="KOG1685">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063901_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKL0"/>
    <dbReference type="OMA" id="ADFRVHH"/>
    <dbReference type="OrthoDB" id="10251727at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023674">
        <property type="entry name" value="Ribosomal_uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028364">
        <property type="entry name" value="Ribosomal_uL1/biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016095">
        <property type="entry name" value="Ribosomal_uL1_3-a/b-sand"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00687">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56808">
        <property type="entry name" value="Ribosomal protein L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR24011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="270" mass="30413" checksum="65F8F6CBECD3C0FC" modified="2009-07-28" version="1">MAVPVVSKSGLAAEALQSLVKRCSEDPKLANDTNIHIVINTEKPIGIKNDYVPRIIPLTSCKMHKPSDLRILLITKDPSTLYRKTLTKDPSTADLFKTILSVRNLKGKYRGSRLSKLFREYDLVVADYRVHHLLPQILGSAFFHSNKKLPFMVKMSQAVKAKREKLPEECDAAYVRAQLRSICKNTFYVPNVDNCLSVKIGEVGVHTVDEMVHNIDDIVRFLTDKSKKPQGGVIRGDISAIFVKTSNSVSLPIYKAEKKSEVHEEEELRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKM7</accession>
    <name>C5DKM7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0F05962p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0F05962g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554465.1">
        <property type="nucleotide sequence ID" value="XM_002554419.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKM7"/>
    <dbReference type="FunCoup" id="C5DKM7">
        <property type="interactions" value="1437"/>
    </dbReference>
    <dbReference type="GeneID" id="8292663"/>
    <dbReference type="KEGG" id="lth:KLTH0F05962g"/>
    <dbReference type="eggNOG" id="ENOG502QRFX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_42_4_1"/>
    <dbReference type="InParanoid" id="C5DKM7"/>
    <dbReference type="OMA" id="ETGKYPL"/>
    <dbReference type="OrthoDB" id="10264707at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052742">
        <property type="entry name" value="Mito_N-acetyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015416">
        <property type="entry name" value="Znf_H2C2_histone_UAS-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43138">
        <property type="entry name" value="ACETYLTRANSFERASE, GNAT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43138:SF2">
        <property type="entry name" value="PROTEIN SPT10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09337">
        <property type="entry name" value="zf-H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="2">
        <location>
            <begin position="91"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="410"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="533"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="417"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="429"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="533"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR24028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="554" mass="62432" checksum="B9DDE7E911D22F56" modified="2009-07-28" version="1">MDSNEGPEELRMTHLQPQSILLKDGETTATMYPIPAYAEQLPAGLLAFLLDEFNMEIEKGDSLPYYEPLTLEGFNETWFHRDGLVCVMVLGEIPELDYSKSEEAGNGSTASDDPQAEVTRHTSQYIRRKERRNLNLNIQWEKQCLGAFNLRPAYPGRSSHVVTATFLVNAGIRGKGIGRTLVECFLSWAPQMGFTSSVFPLVYGTNVGVRRILETLSFRRVGKLPESAILKGCDVPVDSFIYAKEFTHVSKSIDLLNEPNKHFAKYERLRYYLETGKYPDYCDRNEKARLRASSKYHSVQNGKLMRQGREVVYDPEKQLEVAFEVHQVSHQGINKVTTKIAERYHWKGIKTTVARIVAACPLCKTQHQDDLGIVVEAGSPQQQAHMIVPSGGDVDHSSQLSRVTAAVIGSLQEPGTDMERTPVDEEPRKRSKPTIHRIKFSGSRDPQISTQEVLDNEHSMNNFSEYARHQQKSKRRYLDVAMSANMSRSATLPGSDIPSGPATVEIDEQDDTHMVDDALLDLEDNVMAAVEAVRSEQKSKDDQYGHESNVQQFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKT7</accession>
    <name>C5DKT7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">Bis(5'-adenosyl)-triphosphatase</fullName>
            <ecNumber evidence="3 13">3.6.1.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">KLTH0F07414g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Cleaves A-5'-PPP-5'A to yield AMP and ADP. Can cleave all dinucleoside polyphosphates, provided the phosphate chain contains at least 3 phosphates and that 1 of the 2 bases composing the nucleotide is a purine. Is most effective on dinucleoside triphosphates. Negatively regulates intracellular dinucleoside polyphosphate levels, which elevate following heat shock.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 13">
            <text>P(1),P(3)-bis(5'-adenosyl) triphosphate + H2O = AMP + ADP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13893"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58529"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.1.29"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.29" evidence="3 13"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554525.1">
        <property type="nucleotide sequence ID" value="XM_002554479.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKT7"/>
    <dbReference type="FunCoup" id="C5DKT7">
        <property type="interactions" value="186"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKT7"/>
    <dbReference type="GeneID" id="8292729"/>
    <dbReference type="KEGG" id="lth:KLTH0F07414g"/>
    <dbReference type="eggNOG" id="KOG3379">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056776_7_2_1"/>
    <dbReference type="InParanoid" id="C5DKT7"/>
    <dbReference type="OMA" id="QVVHQFI"/>
    <dbReference type="OrthoDB" id="680339at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047710">
        <property type="term" value="F:bis(5'-adenosyl)-triphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01275">
        <property type="entry name" value="FHIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.428.10:FF:000011">
        <property type="entry name" value="Fragile histidine triad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051884">
        <property type="entry name" value="Bis(5'-adenosyl)-TPase_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039383">
        <property type="entry name" value="FHIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019808">
        <property type="entry name" value="Histidine_triad_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011146">
        <property type="entry name" value="HIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46243">
        <property type="entry name" value="BIS(5'-ADENOSYL)-TRIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46243:SF1">
        <property type="entry name" value="BIS(5'-ADENOSYL)-TRIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01230">
        <property type="entry name" value="HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00892">
        <property type="entry name" value="HIT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51084">
        <property type="entry name" value="HIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HIT" evidence="15">
        <location>
            <begin position="3"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="137"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Histidine triad motif" evidence="12">
        <location>
            <begin position="96"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-AMP-histidine intermediate" evidence="9">
        <location>
            <position position="98"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="91"/>
            <end position="94"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for induction of apoptosis" evidence="11">
        <location>
            <position position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR639383-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR639383-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR639383-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU366076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAR24088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="182" mass="21200" checksum="85211FEA7B789E64" modified="2009-07-28" version="1">MTSAVYFSKFLVTKQVFYKSKHSYALVNLKPLVPGHVLVVPLKTSVVNLADLSREENEDFFNTVQLIHRFIKHHYKADSLNIAIQDGPEAGQTVPHLHTHIIPRYRTNNVGDKIYEMIDNWSYESWEERRREYLSSGGREGRKQLAKPDDQRVESSEERMEQEAVELSKELARFLENNPTLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKY1</accession>
    <name>C5DKY1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">KLTH0F08426p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0F08426g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CSC1 (TC 1.A.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554569.1">
        <property type="nucleotide sequence ID" value="XM_002554523.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKY1"/>
    <dbReference type="FunCoup" id="C5DKY1">
        <property type="interactions" value="96"/>
    </dbReference>
    <dbReference type="GeneID" id="8292775"/>
    <dbReference type="KEGG" id="lth:KLTH0F08426g"/>
    <dbReference type="eggNOG" id="KOG1134">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002458_0_2_1"/>
    <dbReference type="InParanoid" id="C5DKY1"/>
    <dbReference type="OMA" id="CSCKKEN"/>
    <dbReference type="OrthoDB" id="1689567at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005227">
        <property type="term" value="F:calcium-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045122">
        <property type="entry name" value="Csc1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003864">
        <property type="entry name" value="CSC1/OSCA1-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027815">
        <property type="entry name" value="CSC1/OSCA1-like_cyt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032880">
        <property type="entry name" value="CSC1/OSCA1-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018">
        <property type="entry name" value="PROBABLE MEMBRANE PROTEIN DUF221-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018:SF5">
        <property type="entry name" value="RE44586P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14703">
        <property type="entry name" value="PHM7_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02714">
        <property type="entry name" value="RSN1_7TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13967">
        <property type="entry name" value="RSN1_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="39"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="116"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="176"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="435"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="480"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="530"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="573"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="621"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="648"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="685"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like N-terminal transmembrane" evidence="9">
        <location>
            <begin position="35"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like cytosolic" evidence="10">
        <location>
            <begin position="219"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like 7TM region" evidence="8">
        <location>
            <begin position="435"/>
            <end position="704"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF14703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR24132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="784" mass="88921" checksum="BFAB39AFA3EABE8C" modified="2009-07-28" version="1">MGTFFDIVQTLSVPEIESLSDGGHSDFRKPSTRVVTTQLLIALSLGLFALLCFSLLLKKLPRLYASRMGRNHGSLPTWDENSLFSWLPVLYRITDSQVLEFAGLDAYVFLGFFKMCIKLLAICCAFSVCLISPIRHHFTGRYDDGNDEASFLLKSSVQDGVDDGSNGGQAPEHAEVYLWMYVIFTSFFTLVALKLLIEQTKNVVVTRQSFLGKQNTLTDRTIRLTGIPVELRDPEALKARIEELKIGKVASVSICREWGPLNNLFNYRKKVLQKLELKYSECPEALRDLEHFDENYRLRSETSLSSDEQLGLNHDSAFAPDAPDSSRIYSDIQLRGRQKIRTGLFGIFGERVDAIDYLEKQLKFIDGEIIEARKKTYPATPTAFVTMDSVANAQMAAQAVLDPRVHFFMTRLAPAPHDIKWDNVCLSRKERLVKVWSVTIFIGVCSLFLLIPVSYLATLLNLKTLSKFWPQLGQYLKKNHWAQTLVTSLLPTYLFTLLNVGIPYFYEFLTSRQGLVSYSEEETSLVSKNFFYIFVNLFLVFTLAGTASNYWGYLSDTTKIAYQLATSVKEFSLFYTDLIILQGVGMFPFKLLLAGSLIGFPFIKIKCKTPRQESEMLRPPIFNFGLQLPQPILILIVTLIYSVMSTKILTSGLAYFVIGFYVYKYQLVFATDHLPHSTGKVWPLIFRRVILGLLLFQLTMAGTLAGFQGGWVLSCCLAPLPLITLSFLWDFEKNYLPLSNFIALSSIREHERDNSTVSSPFESGNYQYPYLVAPLERPIVRRAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>C5DL54</accession>
    <name>C5DL54_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0F10120p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0F10120g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554642.1">
        <property type="nucleotide sequence ID" value="XM_002554596.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DL54"/>
    <dbReference type="FunCoup" id="C5DL54">
        <property type="interactions" value="25"/>
    </dbReference>
    <dbReference type="GeneID" id="8292852"/>
    <dbReference type="KEGG" id="lth:KLTH0F10120g"/>
    <dbReference type="eggNOG" id="ENOG502S1CR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076662_0_0_1"/>
    <dbReference type="InParanoid" id="C5DL54"/>
    <dbReference type="OMA" id="NWNVDMT"/>
    <dbReference type="OrthoDB" id="4036611at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="267"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="164"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="276"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="333" mass="37439" checksum="815FE2FE0F38EE06" modified="2009-07-28" version="1">MSEEEEKRLKQLEEARRRVQELKNKKKKKDKKKKTKGEENESEVGNASENTSEAVEERVDVAPDANAAHSDDTAEKLDNRAPEIVNVESSVETQESEGTSEALSVPKNDVQEVETATTENEEKGAEEGLINEEEKHKDEEQADVEVENAQSAEDNTVEAALEGTGQNSAETETIVEQQRSDDQATELFSSDNDNKDSDFLTELQRENDRLALIDLEKQVSELSSQLRSLKFVNMEQETTVEELQERVHELEQEVSVSQQELASVKQELMQEKQKTARSAASTEPSLLQTRSSTFSPYANNNPVVDRAAIDKWKNWNVDMTAWRSIGSGPVVHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DL62</accession>
    <name>C5DL62_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Signal recognition particle subunit SRP14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Signal recognition particle 14 kDa protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0F10296g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the signal recognition particle (SRP) complex, a ribonucleoprotein complex that mediates the cotranslational targeting of secretory and membrane proteins to the endoplasmic reticulum (ER).</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of a fungal signal recognition particle (SRP) complex that consists of a 7SL RNA molecule (scR1) and at least six protein subunits: SRP72, SRP68, SRP54, SEC65, SRP21 and SRP14.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the SRP14 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554650.1">
        <property type="nucleotide sequence ID" value="XM_002554604.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DL62"/>
    <dbReference type="FunCoup" id="C5DL62">
        <property type="interactions" value="74"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DL62"/>
    <dbReference type="GeneID" id="8292860"/>
    <dbReference type="KEGG" id="lth:KLTH0F10296g"/>
    <dbReference type="eggNOG" id="KOG1761">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094309_3_0_1"/>
    <dbReference type="InParanoid" id="C5DL62"/>
    <dbReference type="OMA" id="DKFWQDY"/>
    <dbReference type="OrthoDB" id="19209at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005786">
        <property type="term" value="C:signal recognition particle, endoplasmic reticulum targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030942">
        <property type="term" value="F:endoplasmic reticulum signal peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.720.10">
        <property type="entry name" value="Signal recognition particle alu RNA binding heterodimer, srp9/1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003210">
        <property type="entry name" value="Signal_recog_particle_SRP14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009018">
        <property type="entry name" value="Signal_recog_particle_SRP9/14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12013">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 14 KD PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02290">
        <property type="entry name" value="SRP14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54762">
        <property type="entry name" value="Signal recognition particle alu RNA binding heterodimer, SRP9/14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 7" id="KW-0733">Signal recognition particle</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU368100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR24213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="137" mass="15162" checksum="5978B174E81D6D98" modified="2009-07-28" version="1">MSTSPHSTPEEFLKAVPDVFALANEGQNSINLSVKRLVTQDPVEGNDEFNASELPQFDVSKKSQFTKVPQNANNKQYDVLVRLKKGSGEGVIKHSTVVKAEALDKFWKDYSAAIKSGMVGLVKKKKKKAKAKKSQRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5DL70</accession>
    <name>C5DL70_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0F10472p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0F10472g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554658.1">
        <property type="nucleotide sequence ID" value="XM_002554612.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DL70"/>
    <dbReference type="FunCoup" id="C5DL70">
        <property type="interactions" value="693"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DL70"/>
    <dbReference type="GeneID" id="8292868"/>
    <dbReference type="KEGG" id="lth:KLTH0F10472g"/>
    <dbReference type="eggNOG" id="KOG3041">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062658_0_1_1"/>
    <dbReference type="InParanoid" id="C5DL70"/>
    <dbReference type="OMA" id="NDPGLCN"/>
    <dbReference type="OrthoDB" id="10249920at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047631">
        <property type="term" value="F:ADP-ribose diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006753">
        <property type="term" value="P:nucleoside phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019693">
        <property type="term" value="P:ribose phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18888">
        <property type="entry name" value="NUDIX_ADPRase_Nudt5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000016">
        <property type="entry name" value="ADP-sugar pyrophosphatase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11839:SF1">
        <property type="entry name" value="ADP-SUGAR PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11839">
        <property type="entry name" value="UDP/ADP-SUGAR PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="2">
        <location>
            <begin position="80"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR24221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="234" mass="26025" checksum="AEA63188D8C0F15C" modified="2009-07-28" version="1">MIRTCSRNLYDTARPLSSRTIGIKRMSSKGRPENAKFLKAHPVSNADECKWIGLERIEYLDPNGTKRQWDSAVRRTRNSGGVDGVGILAILRAPGQDPEILLQKQFRPPVEGVCIEMPAGLIDSEESVDMAALRELKEETGYSGKIVSKTPTIFNDPGFTNTNLSLVTVEIDTSAPENQNPQTQLEDSEFIECIKVPLKNFADEMDNLAKQGYKLDARVQNVAQGIRLAQKYSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>C5DL82</accession>
    <name>C5DL82_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0F10780p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0F10780g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554670.1">
        <property type="nucleotide sequence ID" value="XM_002554624.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DL82"/>
    <dbReference type="FunCoup" id="C5DL82">
        <property type="interactions" value="168"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DL82"/>
    <dbReference type="GeneID" id="8292882"/>
    <dbReference type="KEGG" id="lth:KLTH0F10780g"/>
    <dbReference type="eggNOG" id="ENOG502S9WN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119596_0_0_1"/>
    <dbReference type="InParanoid" id="C5DL82"/>
    <dbReference type="OrthoDB" id="4059443at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008409">
        <property type="entry name" value="SPF27"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05700">
        <property type="entry name" value="BCAS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0747">Spliceosome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="51"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="69"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR24233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="194" mass="21868" checksum="33B6029DD9D8F08C" modified="2009-07-28" version="1">MNGIVDWLPHVDGIETSYRAKAEELVDLELQSLDQQGLHPDIAHLIGQPVPARRGHDFGATKSLRGSKRPAEAAKPEKTSRRKMITDSLEEPEHNYSEQEGAIVESYLGHQLSVLERLLPQTLAAQWATNNEYMSSAQAALTEVIEAQETQLRDLDAHRETLQEQEGGELNYLEQQWRDALVQNVERAIQLSHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>C5DL91</accession>
    <name>C5DL91_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0F11000p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0F11000g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554679.1">
        <property type="nucleotide sequence ID" value="XM_002554633.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DL91"/>
    <dbReference type="FunCoup" id="C5DL91">
        <property type="interactions" value="32"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DL91"/>
    <dbReference type="GeneID" id="8292892"/>
    <dbReference type="KEGG" id="lth:KLTH0F11000g"/>
    <dbReference type="eggNOG" id="KOG0929">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008280_0_0_1"/>
    <dbReference type="InParanoid" id="C5DL91"/>
    <dbReference type="OMA" id="LYEIRRN"/>
    <dbReference type="OrthoDB" id="430364at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032012">
        <property type="term" value="P:regulation of ARF protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1000.11">
        <property type="entry name" value="Arf Nucleotide-binding Site Opener,domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023394">
        <property type="entry name" value="Sec7_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000904">
        <property type="entry name" value="Sec7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035999">
        <property type="entry name" value="Sec7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663:SF405">
        <property type="entry name" value="ARF GUANINE NUCLEOTIDE EXCHANGE FACTOR SYT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663">
        <property type="entry name" value="GUANYL-NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01369">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00222">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48425">
        <property type="entry name" value="Sec7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50190">
        <property type="entry name" value="SEC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SEC7" evidence="3">
        <location>
            <begin position="334"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="686"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="213"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="107"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="170"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="228"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="309"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR24242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="973" mass="108081" checksum="AEA26BA23F18E006" modified="2009-07-28" version="1">MNHALAEIFKSRLFHADHKRRTKDHAESTEVPINTAEPQQQPDNPRILKSIEEEERPLAESGASDDGSEESSHSGKGPGSNKFRSLRKRITSFPDLRFRSASSLSSASEGSLAGLSDKQQIAGSAKSPAKKWINGRAADRRVSSSPHLNLSEKPPIINITNDTGVSFEEESLKVPRKSSSEKRLSSTSITRISTASSAGSSIFARQPRRVLIRSNSDNCQPKKVSPLTPYSNPSPFATTLDEAPVSGITRRRSRTVGASDYGKTNISLHTGLSGGRVGSDSNLREQAVTEGSVCTSAASRPTHLRQHSSSRVSTTTSRRSSSLVNAISNFVSLRSPSVSSRNSLQKAVIDLEDLPKPPTPNENDTPETYLRSLGGFGKYLAVILASDDSGFKIDCLDIFLENEFDFHLEPLDISLRKLLMFLELPKESQQIDRVLTAFSKSYYKQNNKVCIWDTDEDVYSIVFSLLMLHTDAFNVNNKTKMTKQDFVKLMRADAESGGSFTPKEILEYFYDNVTSKEFSKNDYLIYGSEDEIEENYFQNSETGYSPKEIIQNRLLISCASTPLSPAGFSSSGYSLSPYKASSPYSNSTSLGGTSSKHESLDIYYHIASNTLASVTLGAQLKCLPLSEELQKDDELSSTQYAKYLSVIKETKGGYLRLRKAHLESLFEANLEVISPAKEGDDYQYLKIVQMGNLEELVTNRKFSLVGSVNRRVWKRQFGILTTSCLFLFNSMDWVEPQLLVDSTTKISNYIIDCPPQLQINHKFGCNGLFATSINHVPTIEGNAAGGVVIGHSEMYVYSGSKRFVFSCPNDSDRQKWIDSINVAAAFDGCFIEVSAIPDTVVTMRKATVAEKIQKLQGNGFQREERLRRLQSLIAFVHQSIPLNSKIRTALAEYLSQLEKRLDWQLYEIRRNDVYSKILKELGPAPEDNSSQCDTSADQSFAFSEDLERCPTNYSTEHLSDSSINLSGIMGEFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLD8</accession>
    <name>C5DLD8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Catalase</fullName>
            <ecNumber evidence="14">1.11.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">KLTH0F12210g</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes the degradation of hydrogen peroxide (H(2)O(2)) generated by peroxisomal oxidases to water and oxygen, thereby protecting cells from the toxic effects of hydrogen peroxide.</text>
    </comment>
    <comment type="function">
        <text evidence="2">Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>2 H2O2 = O2 + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:20309"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="EC" id="1.11.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the catalase family.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.6" evidence="14"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554726.1">
        <property type="nucleotide sequence ID" value="XM_002554680.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLD8"/>
    <dbReference type="FunCoup" id="C5DLD8">
        <property type="interactions" value="786"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLD8"/>
    <dbReference type="GeneID" id="8292943"/>
    <dbReference type="KEGG" id="lth:KLTH0F12210g"/>
    <dbReference type="eggNOG" id="KOG0047">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010645_2_0_1"/>
    <dbReference type="InParanoid" id="C5DLD8"/>
    <dbReference type="OMA" id="FRYSVND"/>
    <dbReference type="OrthoDB" id="6880011at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004096">
        <property type="term" value="F:catalase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042542">
        <property type="term" value="P:response to hydrogen peroxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08157">
        <property type="entry name" value="catalase_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.180.10:FF:000013">
        <property type="entry name" value="Catalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.180.10">
        <property type="entry name" value="Catalase core domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018028">
        <property type="entry name" value="Catalase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024708">
        <property type="entry name" value="Catalase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024711">
        <property type="entry name" value="Catalase_clade1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011614">
        <property type="entry name" value="Catalase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002226">
        <property type="entry name" value="Catalase_haem_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010582">
        <property type="entry name" value="Catalase_immune_responsive"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020835">
        <property type="entry name" value="Catalase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11465">
        <property type="entry name" value="CATALASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11465:SF62">
        <property type="entry name" value="CATALASE T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00199">
        <property type="entry name" value="Catalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06628">
        <property type="entry name" value="Catalase-rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038928">
        <property type="entry name" value="Catalase_clade1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00067">
        <property type="entry name" value="CATALASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01060">
        <property type="entry name" value="Catalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56634">
        <property type="entry name" value="Heme-dependent catalase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00437">
        <property type="entry name" value="CATALASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00438">
        <property type="entry name" value="CATALASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51402">
        <property type="entry name" value="CATALASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 13 14" id="KW-0349">Heme</keyword>
    <keyword evidence="11 14" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="10 13 14" id="KW-0408">Iron</keyword>
    <keyword evidence="8 13 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6 14" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Catalase core" evidence="16">
        <location>
            <begin position="14"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="62"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="135"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="13">
        <location>
            <position position="349"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR038928-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR038928-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM01060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAR24289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="555" mass="63059" checksum="6E01C9AFE8A7559D" modified="2009-07-28" version="1">MIEKKDSTGQQVYALANGIPYSHHPYGSQVSRPDGPILLQDVHLIESIAHFDRERVPERVVHAKGGGCRLEFELTDSLSDITFAAPYQKPGYKCPGVVRFSTVGGESGTPDTARDPRGFSVKFYTEWGNHDWVFNNTPVFFIRDANKFPHFIHTQKRDPRSHLNHGNDSTMYWDYLTQNLESIHQITYMFGDRGTPADWAQMSGYSGHTFKFFNEKGELTYVQIHVRPDEGFKTLTDQQAAELSGANPDYNTRKLFDRLESGERPTYTCYVQTMTPAQAENFRYSINDLTKIWPHKEFPLRKFGKITLTENVDNYFEEIEQIAFSPSNTCVPGIEPSNDNVLQSRLFSYPDTQRHRLGANYTQLPVNSPRNMCPSAPGSSGCPFLMGNFQRDGPGCIYNQNSYPNYISTHYKERVQFKNLLSDKRSHSKYTGVVLDETSKAAVQQQEDYKKHNEIVDGKINEYQWVTGCSPLDLEQPRALYEKVYDAGAKKRFIENVVGHATKISDDIVKSRVPQYFGLLNQDLGSAIAKGLGIKYEPVSFEEYAHQSASLASAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLE8</accession>
    <name>C5DLE8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0F12496p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0F12496g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554736.1">
        <property type="nucleotide sequence ID" value="XM_002554690.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLE8"/>
    <dbReference type="FunCoup" id="C5DLE8">
        <property type="interactions" value="570"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLE8"/>
    <dbReference type="GeneID" id="8292956"/>
    <dbReference type="KEGG" id="lth:KLTH0F12496g"/>
    <dbReference type="eggNOG" id="ENOG502QUE5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1065852_0_0_1"/>
    <dbReference type="InParanoid" id="C5DLE8"/>
    <dbReference type="OMA" id="SHERAKP"/>
    <dbReference type="OrthoDB" id="2285533at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14688">
        <property type="entry name" value="bZIP_YAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050936">
        <property type="entry name" value="AP-1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018287">
        <property type="entry name" value="Hap4_TF_heteromerisation"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40621:SF7">
        <property type="entry name" value="BZIP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40621">
        <property type="entry name" value="TRANSCRIPTION FACTOR KAPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10297">
        <property type="entry name" value="Hap4_Hap_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="7">
        <location>
            <begin position="76"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="53"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="95"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="73"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR24299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="261" mass="28799" checksum="F1B4D47B4306DA66" modified="2009-07-28" version="1">MSRPRASTHYFLTLHHGVMNYSALRPSVSNSGASASCGELRTSKTWVLPAKTKPGRRAKTCTQQDGGSVSLEDDSRKAKNREAQRAYRERQAGHIKELQDLVEKWRMKCEGLKAELRNRNFELEQHKARVQDLEKKVETLEAERKPVPCDLCALKVTDAGATTGFLDPKLQDHIDNFKPMKAVPLSSGRKKRKWSPSALPTFKRPDERGISAGSLLELPVVDDSLGCGFCSESSACLCKDLAAQPDPAEPAAKRREAGQGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLJ5</accession>
    <name>C5DLJ5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0G01210p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0G01210g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555093.1">
        <property type="nucleotide sequence ID" value="XM_002555047.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLJ5"/>
    <dbReference type="FunCoup" id="C5DLJ5">
        <property type="interactions" value="155"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLJ5"/>
    <dbReference type="GeneID" id="8293351"/>
    <dbReference type="KEGG" id="lth:KLTH0G01210g"/>
    <dbReference type="eggNOG" id="KOG1477">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_308189_0_0_1"/>
    <dbReference type="InParanoid" id="C5DLJ5"/>
    <dbReference type="OMA" id="RHNERQI"/>
    <dbReference type="OrthoDB" id="25503at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013144">
        <property type="entry name" value="CRA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024964">
        <property type="entry name" value="CTLH/CRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006595">
        <property type="entry name" value="CTLH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050618">
        <property type="entry name" value="Ubq-SigPath_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12864">
        <property type="entry name" value="RAN BINDING PROTEIN 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10607">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00757">
        <property type="entry name" value="CRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50897">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="2">
        <location>
            <begin position="234"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="CTLH" evidence="3">
        <location>
            <begin position="635"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="263"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="184"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="263"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR24656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="823" mass="93569" checksum="1DCA3CAE90F82290" modified="2009-07-28" version="1">MSFCMDQVDCAYVRALFPAYLLEQPIGHELWKIYLRNRKLFHKLKNKGQSVPVLKEARLHKLKPNVSHATRKEIWEKLMNLGVLGTVPYNSVGDEYLMQVYQYFYPDIDQDSFGDSRRNEAADEMQIDSDSADLTKPVDPIAHLVRRLGEDDDGEDDDEDLDDDDDDDDDDEDVTQAAERDDQVGDDEDEEDDDDNIEDIHQTEEHRGASDQKLQDSEKDQYFSPLTESSHQNRPKKNYVSMDIYKLLGYPLPHAWDAQSSNSMMVSSDGSNQLKPNPSLEGFPRRSDSIVGSGFSVSYSSYSAKFEYVSARANNSILSKKMAIFYYEIRVLSVTSSQSGQNCNIKVGFKDLSKFQAGSASPSRTDLHAGDPVSINRQSASIMRSTVESSSTTSTKSRGDGFDKGSFGYCGQDGYITDGTQYKIYGKQYGRGDVIGCGVNFVDGTIFFTKNGINLGTAFTDVHDLDLVPFIALKPGNMVRTNFGLHEEFVFDILNYQRMLKSKAYHHIYKSIDGHDGRNDFESSIHSDQDDFDDDPPLSGDNRGGFLLQNDERFSGDKLYRPEVEKLNNLSTDDDSIPCAMHAMINDHLIHEGLIDVAKGFLKDLQKDCIPNSDEERARLVIRHNERQIIKEENNLKLRQSIRRFINEGDIAQCLVFINSQYPGLIEKNVDLLFELKVAEYLLMFVQFKSHSIDEILRKGQELTSEFVYSQQVPADYKDGFRNHLNDISSLLAYDNPLRECNEDLAIYLTPAYLQDRLFQLVNSRVLAFLEKKSESSLESMVSYTRAMIDTLMEYGEGSSLVHNDSELRFHKLVNIDEDLLNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DM12</accession>
    <name>C5DM12_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0G05104p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0G05104g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555260.1">
        <property type="nucleotide sequence ID" value="XM_002555214.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DM12"/>
    <dbReference type="FunCoup" id="C5DM12">
        <property type="interactions" value="804"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DM12"/>
    <dbReference type="GeneID" id="8293526"/>
    <dbReference type="KEGG" id="lth:KLTH0G05104g"/>
    <dbReference type="eggNOG" id="ENOG502S1NP">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025391_0_0_1"/>
    <dbReference type="InParanoid" id="C5DM12"/>
    <dbReference type="OMA" id="LKMIKTS"/>
    <dbReference type="OrthoDB" id="9439903at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051643">
        <property type="entry name" value="Transcr_Reg_ZincFinger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24396:SF19">
        <property type="entry name" value="FI01119P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24396">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="159"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="38"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="352"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="39"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="55"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="356"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR24823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="538" mass="59997" checksum="B93AC422E280EC6F" modified="2009-07-28" version="1">MPYVSLLKPSQNKILVSIAEKVKEFFRNLADFVVAGDGNSKDAKKDVEEGINEAEEVKNSPEKSRSLFPAQNNVDKSLELAPTIVPCALDLSTLQSPISMTKTPEGHLDWQLMAPSMTGDAAGPNGPPSFAPTLNSPESIECKTEDDEHGCSDKDQKRFVCHYCNAKFYIRGYLTRHIKKHAVEKAYYCPYFNADAPKDQRCHTTGGFSRRDTYKTHLRSRHFICPKGVRSQEKAKSSGRCAHCNEQFDKTDDWIKSHVEAGECKGLPDGFKVAVKSSRKTGKLKMIKTSNGHSRFISTQQCVIEPSVMHNKEALEATAIVIDQSKNNGNPLESTVLTTNDNKIMLNSEHFQGASKTKKNNKVSPKKPEVASLDGPQDSFFNYPNSQASLGTAQPHLQSYPFSQVTPVKETSLDEGCLSMDPSPTEDAGLEAVNSSSSASSRVSFHDHNVKDNQASSMPYPNAHDDFFQFPLDIDQCPMNPMYYERPVQSHNEGLASEGSQINETLNKQMDAVVLSERHFRENQQYLNFYNYTFDSHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DM18</accession>
    <name>C5DM18_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0G05236p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0G05236g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555266.1">
        <property type="nucleotide sequence ID" value="XM_002555220.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DM18"/>
    <dbReference type="FunCoup" id="C5DM18">
        <property type="interactions" value="136"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DM18"/>
    <dbReference type="GeneID" id="8293532"/>
    <dbReference type="KEGG" id="lth:KLTH0G05236g"/>
    <dbReference type="eggNOG" id="KOG2852">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007884_14_0_1"/>
    <dbReference type="InParanoid" id="C5DM18"/>
    <dbReference type="OMA" id="DDTVYAC"/>
    <dbReference type="OrthoDB" id="498204at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000237">
        <property type="entry name" value="FAD dependent oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847:SF150">
        <property type="entry name" value="OXIDOREDUCTASE TDA3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847">
        <property type="entry name" value="SARCOSINE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="3">
        <location>
            <begin position="27"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="3">
        <location>
            <begin position="236"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="176"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR24829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="492" mass="53650" checksum="FCBBF5C644399D86" modified="2009-07-28" version="1">MSPSTHTVDQIEFLSRPSGKAEGKHHIVIVGAGIIGVCIAYYLTRHPSFDPKKHHITVLESKRVAGGASGKAGGLLATWAFPQQIVPLSFQLHQELSDEFDGENQWDYRRLTTVSLEADVQHVSEATDKDDANSTKSADSRSHTLSTRKKNKEPRSSVPKKKRCAQTTRSDRDGSDGDDESDDEDNDELESKEMGPEGLQESYSMGANQPPAPVLPEDLNWIRTQLVRDWSSLGGADSTAQVHPYKFTHFILHEAMKTGAVDLILGKVNEIKFNDVGCASGISYIPTSDDYDGEPDAVETLEAQQIVLAMGPWTSKILPDCPISGLRAHSITIKPSTGTVSPYAIFTELKIAHNNYFSPEMYARKDEVYVCGEGDTLVELPETSDAVEVVREKCDELYHYVSKLSPNLSQGHILKRQACYLPVLNVPTSSGPLIGETNMEGLYVASGHSCWGINNAPGTGKIMSELLLDGAAKSADISALDPGLYFDASVLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DM23</accession>
    <name>C5DM23_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0G05346p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0G05346g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL27 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555271.1">
        <property type="nucleotide sequence ID" value="XM_002555225.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DM23"/>
    <dbReference type="FunCoup" id="C5DM23">
        <property type="interactions" value="1239"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DM23"/>
    <dbReference type="GeneID" id="8293537"/>
    <dbReference type="KEGG" id="lth:KLTH0G05346g"/>
    <dbReference type="eggNOG" id="KOG3418">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067359_0_1_1"/>
    <dbReference type="InParanoid" id="C5DM23"/>
    <dbReference type="OMA" id="NQWFFTK"/>
    <dbReference type="OrthoDB" id="2365484at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06090">
        <property type="entry name" value="KOW_RPL27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.770:FF:000001">
        <property type="entry name" value="60S ribosomal protein L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001141">
        <property type="entry name" value="Ribosomal_eL27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041991">
        <property type="entry name" value="Ribosomal_eL27_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038655">
        <property type="entry name" value="Ribosomal_eL27_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10497">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01777">
        <property type="entry name" value="Ribosomal_L27e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="KOW" evidence="4">
        <location>
            <begin position="8"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR24834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="136" mass="15549" checksum="B9277324C6131FF9" modified="2009-07-28" version="1">MAKFLKAGKVAVVVRGRYAGKKVVIVKPHDEGSKAHPFGHALVAGIERYPLKVTKRQGAKKVAKRTRIKPFIKVINYNHLLPTRYTLDVEGFKSVVSTETFEEPSQREEAKKVIKKAFEERHQAGKNQWFFTKLNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>C5DM80</accession>
    <name>C5DM80_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0G06688p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0G06688g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555328.1">
        <property type="nucleotide sequence ID" value="XM_002555282.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DM80"/>
    <dbReference type="FunCoup" id="C5DM80">
        <property type="interactions" value="45"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DM80"/>
    <dbReference type="GeneID" id="8293597"/>
    <dbReference type="KEGG" id="lth:KLTH0G06688g"/>
    <dbReference type="eggNOG" id="ENOG502QWA5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042082_0_0_1"/>
    <dbReference type="InParanoid" id="C5DM80"/>
    <dbReference type="OMA" id="ADWNIYT"/>
    <dbReference type="OrthoDB" id="4138492at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="557" mass="61747" checksum="DADA54501EC942BA" modified="2009-07-28" version="1">MLYVSLKLITTISLVVVASVWFGSKLVINSRRDLHPIALNGQANVGPTRKEKETAVYRNFTVPPGFPLSTGLGLSMGYKLRNGNFGDIWSNIMSLSAENFVELSGQEYSVKKINAISKCLSKYVVQHSESGRLGIAAPIFELPGFTISIAGLMSSLNDVIPVFLSSVPRSKQELDILAIDSWTTFHQMNGSHKWYKIVIVCNDHSGQPPQTIPPNVVTWKDIVSNQEEDTVFEYEPPKDNSDDTKLLAIVSSPWNLSTSFSHMALVSSVSAFIKSFPMDNELSGKDHLTVVIEEATKYYYPIQVWTKILTTLLHGGSASLLYAEPKQVDLQSCINEKTSLLQIDSSSPLLDSILQSSTAVLQELKLSWALSLLSEGIFTPSALSCSAFHNIRCLFLSTTVLDARAVTAMEMPSRKVQTKAKNSRSTLTLNKLRALIGSRLVVELYSPFMVMGPIACTNFFDYRILPDSVDSRLSHYGPISTSLEAKLVEDEHRPELQSSKRQGMLCIRGFTIGKPVEPERLERALQIVEKFDGGEGWMPMLGVYGLFGKDGCFYEFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMD6</accession>
    <name>C5DMD6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0G07986p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0G07986g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MNN1/MNT family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555384.1">
        <property type="nucleotide sequence ID" value="XM_002555338.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMD6"/>
    <dbReference type="GeneID" id="8293655"/>
    <dbReference type="KEGG" id="lth:KLTH0G07986g"/>
    <dbReference type="eggNOG" id="ENOG502RZ48">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015387_1_0_1"/>
    <dbReference type="InParanoid" id="C5DMD6"/>
    <dbReference type="OMA" id="CEMYTIC"/>
    <dbReference type="OrthoDB" id="430354at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000033">
        <property type="term" value="F:alpha-1,3-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022751">
        <property type="entry name" value="Alpha_mannosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31392">
        <property type="entry name" value="ALPHA-1,3-MANNOSYLTRANSFERASE MNN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31392:SF1">
        <property type="entry name" value="ALPHA-1,3-MANNOSYLTRANSFERASE MNN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11051">
        <property type="entry name" value="Mannosyl_trans3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR24947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="569" mass="65153" checksum="35DB61356B2FDF47" modified="2009-07-28" version="1">MRIFTRKLLKVGAAAVCVICLLYAYQSSLHNHVSKSLTEDIAAHLVKDKEPFSSPSEICSQYFRKLYELEPNWWNSYSEGWQLAATDYETENLRLIMERLRTYEKCLEIGALPSNIQDIDKRMFPFIRLEQSPIFESSDLKKAYQNGQFPSMSNGAISKYRYNPQQSWLANWNRTSQETSGPTRGIVLSFPDAHASLVVRLLKVLEHQGNRLPIQIIHKGDLSDYSRSLISRSLPPNQRLWFVNVKPMLDPHFIDDFSLYRNKWLAMIFNTFSTPIFIDADAVSVQPVEDYLQSEAYVESGTIFFRDRAFAHKFDYEYCVPLLKALQPSRLETLYFGQRRLIDEQLMMSDSTSLEQRIVQSFFVGGQRHQMDSGLLAVDKSKHLMPLMAGVFLNLAPKLSKCTHGDKETFWLAFLLTNHAYRFHPEEASAIGEDDGSGEVCSVQLGHTSSHGTLLWFNSGFRVCKFADAIDTDWEGYPKEYLHETFASIQEAREYYESMTNIKAAVLPDISVNPWTGGFADLCIGYRYCARVSPGSGQLINFNAEEQKKMREIGNAWMSATEAELNFRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMI0</accession>
    <name>C5DMI0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0G09108p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0G09108g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555428.1">
        <property type="nucleotide sequence ID" value="XM_002555382.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMI0"/>
    <dbReference type="FunCoup" id="C5DMI0">
        <property type="interactions" value="361"/>
    </dbReference>
    <dbReference type="GeneID" id="8293705"/>
    <dbReference type="KEGG" id="lth:KLTH0G09108g"/>
    <dbReference type="eggNOG" id="ENOG502QSY2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010084_1_0_1"/>
    <dbReference type="InParanoid" id="C5DMI0"/>
    <dbReference type="OMA" id="VENDRCH"/>
    <dbReference type="OrthoDB" id="422427at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051711">
        <property type="entry name" value="Stress_Response_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47540:SF1">
        <property type="entry name" value="ACTIVATOR OF STRESS GENES 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47540">
        <property type="entry name" value="THIAMINE REPRESSIBLE GENES REGULATORY PROTEIN THI5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="9">
        <location>
            <begin position="14"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="627"/>
            <end position="647"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR24991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="782" mass="87678" checksum="FC21F11E1F9E9DE7" modified="2009-07-28" version="1">MSDLQNKRRRVTRACDECRKKKVKCDGQQPCIHCTVYSYECTYNQPSKRVATASASTAAAALTPPYGVSQPSRVKPHKALRDAKLQLSKYKMLFGRLFPQMPPIEQVDVASFANAFQGFVASNEEFQNATKNSHVTVPVSSASGAGSPATASATPEGSVGVDAIEGIDGSIQSHEGREIKIILPPKPVALHFIQVTWEQCCVLFRFYHRPSFIASLDELYETDPHDYTHEQMHFLPLCYAVMAVGALFSKSMQSTGTPSDTASPGKFMQDEGYKYFVAARKLIDITNARDLNSIQAIVMLFIFLQCSARLSTCYTYIGVAMRNALREGMHRNLNADTHSYNPIEIEMRKRLFYTIYKMDVYVNTMLGLPRSVSQRDFDQALPAELTDEFITKDGLHFEKQGNVLSSAGIANQHTKLIMILDNIVAELYPVKKTNNLISHDVVTQLELKLRQWLDNLPPELAPGSNDVPPKYERANQLLHLSFLQVQVILYRPFIHYLSATVPTKAADSLSIRRARNCISVARTVVKLAKKMMEKKTLTGSYWFSIYNIFFSVAGLIFYLHEVAPTDEAGELEYSEIQKDAELGKGVLLYLKDTSMAANRTYNLLNMLFEKFNSKTIKWSQVKQGEFKASAPESSQQAPIKTPPVNMDTEPSTYNLDPGNMDLLAFENPQDATLQSTSTISGVNTMNTINTSNMLMNKDFFGVNDSLEMDKFFNLSENGSLDEKAAPLQPPDIQLKEEYEVPSEQNMSESYVPGAFDQLDVQLFGRYLPPYMSQQNESPKFGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMN3</accession>
    <name>C5DMN3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0G10296p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0G10296g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555481.1">
        <property type="nucleotide sequence ID" value="XM_002555435.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMN3"/>
    <dbReference type="FunCoup" id="C5DMN3">
        <property type="interactions" value="769"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMN3"/>
    <dbReference type="GeneID" id="8293759"/>
    <dbReference type="KEGG" id="lth:KLTH0G10296g"/>
    <dbReference type="eggNOG" id="KOG1188">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037323_3_1_1"/>
    <dbReference type="InParanoid" id="C5DMN3"/>
    <dbReference type="OMA" id="SIHSCGW"/>
    <dbReference type="OrthoDB" id="25131at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039328">
        <property type="entry name" value="WDR89"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22889">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 89"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22889:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 89"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="162"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="376"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="378"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="405"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR25044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="426" mass="48070" checksum="EC4BF5DC4BFE4708" modified="2009-07-28" version="1">MIINLLISQSSRTSRTTYSMSYSKLESIEFGPNNWCLKLQPIYNSGLLTSLSNGRVELIDWATHKSILQIQTHATSVNDMVIINNDRMNGSLIATAAEDAVKIYDLKSNDCVATLKNGKSAPFLSLDSRHGLLGCGTELSGVDAELHVYDIRSWQQPLRSLVDSHHDDITSIKFHPSDPNVLLSGSTDGYVNIYDLTQQEEDDALHQVINFASIHSCGWLSPKRIYTLSHMETYGIHELNDKRDEPTEPKPVDFGDVRKPWDCNYVIDVYPGFIATGKSEEGRGELKLIPLDHEKPELQSAITIPSAHDDEVVRDVLVPFQHEDLLYSCGEDGSLKVWKSSAGPLNVPQEFWQYSEKIDVFSGTVAEVEMMDSEVPDIELRQEPSDVDAVSKDQDEKRKRKHERRSKDKSKSKTKSSSKDHRYQPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMQ7</accession>
    <name>C5DMQ7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0G10846p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0G10846g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPC synthetase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555505.1">
        <property type="nucleotide sequence ID" value="XM_002555459.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMQ7"/>
    <dbReference type="FunCoup" id="C5DMQ7">
        <property type="interactions" value="847"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMQ7"/>
    <dbReference type="GeneID" id="8293783"/>
    <dbReference type="KEGG" id="lth:KLTH0G10846g"/>
    <dbReference type="eggNOG" id="KOG2728">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042326_1_0_1"/>
    <dbReference type="InParanoid" id="C5DMQ7"/>
    <dbReference type="OMA" id="LERYQHH"/>
    <dbReference type="OrthoDB" id="70224at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004632">
        <property type="term" value="F:phosphopantothenate--cysteine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10300:FF:000002">
        <property type="entry name" value="Phosphopantothenate--cysteine ligase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10300">
        <property type="entry name" value="CoaB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035929">
        <property type="entry name" value="CoaB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007085">
        <property type="entry name" value="DNA/pantothenate-metab_flavo_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12290">
        <property type="entry name" value="CORNICHON-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04127">
        <property type="entry name" value="DFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102645">
        <property type="entry name" value="CoaB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA/pantothenate metabolism flavoprotein C-terminal" evidence="2">
        <location>
            <begin position="65"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR25068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="349" mass="39995" checksum="E3D74AF331A7FC1B" modified="2009-07-28" version="1">MPIHTSTTEVARAIDRSLNAELFPPAETADEEKFFLTNPRPEYLDDLTQQARDFIELQYSLGRSRIVLVTSGGTTVPLENNTVRFIDNFSAGTRGASSAEQFLRQGYAVIFLHREFSLTPFNRNFTHNSDVCFLDYFDNSGAVKPQFRQDLLSAKSLFERYMHEERRLLLLPFTTVNQYLWSLKSVAKLLDRKGSLFYLAAAVSDFFVPHSRLPEHKIQSQEVSQPSTLPNSTAHTSVDGKLIVDLDPVPKFLRRLVVSWAAQAMIVSFKLETDESILIKKATQALDRYNHQLVIGNLLQTRNKEVVFVSEQNRKGDWIRVPDDKQAIEELIIPQVISHHDKWIRDQTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMX8</accession>
    <name>C5DMX8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0G12496p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0G12496g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the DOP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555576.1">
        <property type="nucleotide sequence ID" value="XM_002555530.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DMX8">
        <property type="interactions" value="137"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMX8"/>
    <dbReference type="GeneID" id="8293858"/>
    <dbReference type="KEGG" id="lth:KLTH0G12496g"/>
    <dbReference type="eggNOG" id="KOG3613">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001197_1_0_1"/>
    <dbReference type="InParanoid" id="C5DMX8"/>
    <dbReference type="OMA" id="SHFNDYW"/>
    <dbReference type="OrthoDB" id="297643at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040314">
        <property type="entry name" value="DOP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056457">
        <property type="entry name" value="DOP1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007249">
        <property type="entry name" value="DOP1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056458">
        <property type="entry name" value="TPR_DOP1_M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14042">
        <property type="entry name" value="DOPEY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14042:SF24">
        <property type="entry name" value="PROTEIN DOPEY-1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24598">
        <property type="entry name" value="DOP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04118">
        <property type="entry name" value="Dopey_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24597">
        <property type="entry name" value="TPR_DOP1_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="DOP1 N-terminal" evidence="7">
        <location>
            <begin position="19"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="DOP1-like middle TPR" evidence="8">
        <location>
            <begin position="364"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="domain" description="DOP1-like C-terminal" evidence="9">
        <location>
            <begin position="1222"/>
            <end position="1651"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00046326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF24597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF24598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR25139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1676" mass="189756" checksum="8A279D07185A52E9" modified="2009-07-28" version="1">MMSVPLKPLSVDFNSRQLDSKQKKFHASIERALQHFDSVTEWADYIASLGKLLKALQSWTPQFQNVKYYVPFPYQVSRRLASSLSPNLPSGVHQKTIEVYNVIFSKIGIEALNKECNIWVPGILPLMSYASISVKGPLIDLYENYLLRISPSALRLLVKPLVASLFPGIDDDSSEFQPAVFSLLEVLRANVADEPLFWQSCFIVIITNKDRRLGGLAWLTRMLPSLNAVPHKVAQNKLEEDKAKAKGDAKYRRQTALDLLLPEAKNVVHPEPGLLIRCLVNCLGEENELLIKRGVLDLLLQRINLDSPVIQSLASDSDLRMLMMSCCKTMLNRDVSISRRIWNWLLGPASINANQQHQTNANEYFPHFGEQCLKEGLTAMMGDEAQAPVAFRICIAIMDRWEIASRIIPLVFVPLMKTVRGFENNEIVMKNASSFFDSVETNIICSHLFQVTVFDLDLDLLSFVLSTFKVENDEEIVVRHLPLLLMTVLAKRDEFALPDVYRVSALLVNLIPERAFLPIEHTGVLQDSRCNNQDVLKLISEYYTKLLSPATLSNVNDSYDLSPPFSSEDLTYLIVAEFHDVLLKTFNNEREFNKMVGLFVKLMEKIPNDSNSTDDMSSVSAGKGLEQVLVNKLFEIKGELENHPNGSIFGWLKLYTDYLAPKVTVIQSAKLTKTFVVGAWPYLIDPNTQIETVKSLDSLQGSVRGKHVESALAYAFISELGISSRISALEGIWVHSGDASTLARRPLELVLDELFDDQDPNFLSTSRWVLSVVKQGLAGRLFHILCENILNCKLIYKGALEDLDDIDSYIYHCQTLINVLRIEPSIVLHTFNLEQTSVQTLEIWNGADVSTYKNLAIAIAIKFLKTKNTTGKSVRTTLLLLDILLDGTEHNFKHIVIFLLHLATKHFSSGDLESELISVSLLDIISKVLALSHNNQITLGIFEDDASHLRYIDFLVLGVTSMDKPLIIHSYVKLLSESLVYFQNSIFSIILPLTTSITGCINKLFHSTSVKGDRYQSIAYLIDGLQELMEVSHSYLAAEESGGYLSAGSSKNDFLQNMVANVFSNDSNASNSKLKHQREIIVQSFKLVVSCCLNIWSWAHDYSKAREIEDHEPNEGLFEDSLHHQAYKYKYQSKKLLSAVFGLEPLEVLEELISEGPSNVSLTLVHALDGNKPALTLPHLFQGIVYRCNKFSSITFSNTTASKSAPHPLLINQLETQPLLNFVLAYLSALESAALEEIFGDLMLFLKEITSNHQHYKQLAPLVLKMIALVCEKLHNSRFGEDKRVRRELSDAFCRYLPNAVGEDVDINNATLYEDFDFVCSRLKYIVIDYPSGDKFCTCVSLFVQFAVTPYFKKQNDRYIPRHVVRLALTTSKIGSKVKAWKALVSEAFMSDKKFSLVGQSPLCAEVIFEWSNYPENREKLMQELIASIGTKANGISPNINPFTAWSDSEIETKCHNILRVSFLLLISPRDHHLLCFRSIMGQLEQNLISDDQRMKSHSFLLLRCVLLQFSAMHFGEYWSMLAYSLQTGLQDFYEHLQMQQEFPADVVFQVSKCVDLILTLNFEEFSACYEWLFVIDTMNCIYKSDPYISLADEISDFKDFTKSESDDFAMAQDPIVRVPLLQGIHKVEKYTQLRGFFHNLSYAHYEEMYAMKKVDLDSCTSDALADVFALQLHQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMZ7</accession>
    <name>C5DMZ7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0G12980p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0G12980g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CDC45 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555595.1">
        <property type="nucleotide sequence ID" value="XM_002555549.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMZ7"/>
    <dbReference type="FunCoup" id="C5DMZ7">
        <property type="interactions" value="741"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMZ7"/>
    <dbReference type="GeneID" id="8293878"/>
    <dbReference type="KEGG" id="lth:KLTH0G12980g"/>
    <dbReference type="eggNOG" id="KOG2475">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005871_3_0_1"/>
    <dbReference type="InParanoid" id="C5DMZ7"/>
    <dbReference type="OMA" id="EDCFMEA"/>
    <dbReference type="OrthoDB" id="10258882at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031261">
        <property type="term" value="C:DNA replication preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902977">
        <property type="term" value="P:mitotic DNA replication preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003874">
        <property type="entry name" value="CDC45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10507">
        <property type="entry name" value="CDC45-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10507:SF0">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 45 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02724">
        <property type="entry name" value="CDC45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="165"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="165"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="205"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR25158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="637" mass="73150" checksum="814564A0BB6BC9BF" modified="2009-07-28" version="1">MYLKITQFQEAYERILKFSSSHSSCQLVIFVSCLNIDALCASKMLSKLFKKQLIQLQLVPVFGYSELRSHFQKLDENVSSVIFVGCGGMIDIEAFLEINEQDYWEETSSGQKLKRNIYVLDTHRPWNLHNLFGSQIVTCFDDGTVEETLQSQKEAYIKLVELEEQGANDEEEVSSDEDAEEDQDGDTEEDDEQDSDGNGGETAKRRNTQHNARSQRKLRKKLANQYEEILEEYYAQGTSVSNSISVQVYSLLSSQGETSIPYLWLTVLGATSLDTSYPQIYNRLQPILQDEVRRLSPNESYKTPDTISLEIQPDYYLFLLRHSSLYDSFFYSNYVNAKLSLWNENGKKRLHKIFARMGIPLSTAQENWLYMDNRIKKELGSIFDKNLERYGLQDIVRDGFVRTFGYRGAVSASEFVEAITALLEVGQVTNPDEITNRGNANADNDSDQNMRDVLTRRERKWVSNFWLSWDALDDNIDLMRRGIKHAQHLQRCVFNTGVAILEKKMIKNLRLYRLCALQDGPDLDLYRNPLTLLRLGNWIIECCAEAEDRQLLPLVLASLDVTTETYLVAGMAPRYPRGMDKLEAYKPILNNFHAAFQYIAAETGAKVRIDNFESSIIEIKKEDLSPFLEKLTLSGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>C5DN57</accession>
    <name>C5DN57_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Polyadenylate-binding protein</fullName>
            <shortName evidence="14">PABP</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ordered locus">KLTH0G14344g</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Binds the poly(A) tail of mRNA. Appears to be an important mediator of the multiple roles of the poly(A) tail in mRNA biogenesis, stability and translation. In the nucleus, involved in both mRNA cleavage and polyadenylation. Is also required for efficient mRNA export to the cytoplasm. Acts in concert with a poly(A)-specific nuclease (PAN) to affect poly(A) tail shortening, which may occur concomitantly with either nucleocytoplasmic mRNA transport or translational initiation. In the cytoplasm, stimulates translation initiation and regulates mRNA decay through translation termination-coupled poly(A) shortening, probably mediated by PAN.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the polyadenylate-binding protein type-1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555655.1">
        <property type="nucleotide sequence ID" value="XM_002555609.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DN57"/>
    <dbReference type="FunCoup" id="C5DN57">
        <property type="interactions" value="1547"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DN57"/>
    <dbReference type="GeneID" id="8293940"/>
    <dbReference type="KEGG" id="lth:KLTH0G14344g"/>
    <dbReference type="eggNOG" id="KOG0123">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012062_22_4_1"/>
    <dbReference type="InParanoid" id="C5DN57"/>
    <dbReference type="OMA" id="NATYSMA"/>
    <dbReference type="OrthoDB" id="19742at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12378">
        <property type="entry name" value="RRM1_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12379">
        <property type="entry name" value="RRM2_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12380">
        <property type="entry name" value="RRM3_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12381">
        <property type="entry name" value="RRM4_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000003">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000211">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000648">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000590">
        <property type="entry name" value="Polyadenylate-binding protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1900.10">
        <property type="entry name" value="c-terminal domain of poly(a) binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036053">
        <property type="entry name" value="PABP-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006515">
        <property type="entry name" value="PABP_1234"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002004">
        <property type="entry name" value="PABP_HYD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034364">
        <property type="entry name" value="PABP_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045305">
        <property type="entry name" value="RRM2_I_PABPs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01628">
        <property type="entry name" value="PABP-1234"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24012">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00658">
        <property type="entry name" value="MLLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00517">
        <property type="entry name" value="PolyA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63570">
        <property type="entry name" value="PABC (PABP) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51309">
        <property type="entry name" value="PABC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="10 13 14" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="9" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RRM" evidence="17">
        <location>
            <begin position="40"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="17">
        <location>
            <begin position="128"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="17">
        <location>
            <begin position="221"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="17">
        <location>
            <begin position="324"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="PABC" evidence="18">
        <location>
            <begin position="493"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="293"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="27"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU362004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAR25218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="587" mass="65361" checksum="3054AF2BF0750473" modified="2009-07-28" version="1">MSDVTDKTAEQLEQLKIQDEPQTEPTSAESESSKVESSSASLYVGELDPSVTEALLYDLFSPIGSVSSIRVCRDAITKTSLGYAYVNFHDHNAGRTAIEKLNYTPIKGRPCRIMWSQRDPSLRKKGSGNVFIKNLHPAIDNKALHDTFSVFGNILSCKIATDETGKSRKFGFVHFEEEEAAKEAIDAINGMLLNGLEVYVAPHVSKKDRQSKLDEVKSNFTNVYVKNIDSETTQEEFEKMFSNYGAITSAVLERDNEGKLRGFGFVNFEDHNAALKACEELNDTDFKGQKLYVGRAQKKYERLQELKKQYESSRMEKLAKYQGVNLFVKNLDDSVDDQKLEEEFAPFGTITSVKVMRDEAGNSKGFGFVCFSTPEEATKAITEKNQQIVAGKPLYVAIAQRKEVRRSQLAQQIQARNQMRYQQATAAAAAAAGMPGQFMQPMFYGVMPPRGVPFNGPNPQQMAAMNGIPKNGVPPQQFGRPAGPMYGVPPQGPQGAFPRNGNQFFHQKQRQALGEQLYKRVSAKTQDEEAAGKITGMILDLPPQEVVPLLENDELFEQHFKEAFAAYDSFKKDQDAQAQAEPATEQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNC9</accession>
    <name>C5DNC9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Carboxypeptidase</fullName>
            <ecNumber evidence="11">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0G15950g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>Release of a C-terminal amino acid with broad specificity.</text>
            <dbReference type="EC" id="3.4.16.5"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the peptidase S10 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="11"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555727.1">
        <property type="nucleotide sequence ID" value="XM_002555681.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNC9"/>
    <dbReference type="FunCoup" id="C5DNC9">
        <property type="interactions" value="816"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNC9"/>
    <dbReference type="MEROPS" id="S10.A49"/>
    <dbReference type="GeneID" id="8294013"/>
    <dbReference type="KEGG" id="lth:KLTH0G15950g"/>
    <dbReference type="eggNOG" id="KOG1282">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008523_10_4_1"/>
    <dbReference type="InParanoid" id="C5DNC9"/>
    <dbReference type="OMA" id="TSCDDTV"/>
    <dbReference type="OrthoDB" id="443318at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000328">
        <property type="term" value="C:fungal-type vacuole lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046938">
        <property type="term" value="P:phytochelatin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031638">
        <property type="term" value="P:zymogen activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.410:FF:000001">
        <property type="entry name" value="Carboxypeptidase Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033124">
        <property type="entry name" value="Ser_caboxypep_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF51">
        <property type="entry name" value="VACUOLAR SERINE-TYPE CARBOXYPEPTIDASE ATG42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00560">
        <property type="entry name" value="CARBOXYPEPT_SER_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 11" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005125152" description="Carboxypeptidase" evidence="11">
        <location>
            <begin position="20"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00052076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR25290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="496" mass="55340" checksum="38F1D3F286074912" modified="2009-07-28" version="1">MKLSSAAYSLLGLSSIASAFSLLEGLGLESDLAHLWKGLRVPKNTVQSIKNVRESLDEFVTIKSELDDTYSMRVKRVDPSKLGVDSVKQYSGYLDYEDSKHFFYWAFESRNDPLNDPVILWLNGGPGCSSFTGLFFELGPSSVGPELKPVRNPYSWNNNATVIFLEQPLGVGFSYGDERVASTNAAGKDVFIFLELFFQEFPQFRSNDFHIAGESYAGHYIPEIAHQIAVVHESDKTFNLTSIMIGNGITDSLVQYDYYEPMACGRGGYKAVITEEECAKMRNQMPRCRALNNACYSSSSTFACIAAGAYCENMAMSAYTKTGLNVYDIRSPCETEEGGLCYAGLSYVEDYLNQPEVQVALGSDVSNFTGCSNEVGLAFLLTGDNNRPFQQYVAELVNRDIPVLLYAGDKDFICNWLGNLAWSDELEWKHKEQYSVLPLRPWKSEDSGETLGQVKSYSSFTFLRVFGAGHMVPYNQPEASLEMVNRWISGDYSLGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNF7</accession>
    <name>C5DNF7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0G16588p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0G16588g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555755.1">
        <property type="nucleotide sequence ID" value="XM_002555709.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DNF7">
        <property type="interactions" value="1121"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNF7"/>
    <dbReference type="GeneID" id="8294042"/>
    <dbReference type="KEGG" id="lth:KLTH0G16588g"/>
    <dbReference type="eggNOG" id="KOG1823">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000327_0_0_1"/>
    <dbReference type="InParanoid" id="C5DNF7"/>
    <dbReference type="OMA" id="EGLMAMF"/>
    <dbReference type="OrthoDB" id="360653at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052575">
        <property type="entry name" value="SSU_processome_comp_20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057525">
        <property type="entry name" value="UTP20_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046523">
        <property type="entry name" value="UTP20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011430">
        <property type="entry name" value="UTP20_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17695">
        <property type="entry name" value="SMALL SUBUNIT PROCESSOME COMPONENT 20 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17695:SF11">
        <property type="entry name" value="SMALL SUBUNIT PROCESSOME COMPONENT 20 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20416">
        <property type="entry name" value="UTP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23099">
        <property type="entry name" value="UTP20_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07539">
        <property type="entry name" value="UTP20_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="U3 small nucleolar RNA-associated protein 20 N-terminal" evidence="3">
        <location>
            <begin position="833"/>
            <end position="1419"/>
        </location>
    </feature>
    <feature type="domain" description="U3 small nucleolar RNA-associated protein 20" evidence="4">
        <location>
            <begin position="1610"/>
            <end position="1827"/>
        </location>
    </feature>
    <feature type="domain" description="U3 small nucleolar RNA-associated protein 20 C-terminal" evidence="5">
        <location>
            <begin position="2255"/>
            <end position="2484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2463"/>
            <end position="2498"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="2394"/>
            <end position="2421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="2466"/>
            <end position="2481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF20416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR25318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="2498" mass="283579" checksum="8A367FCC708F3904" modified="2009-07-28" version="1">MVKQKVTSKSSKRYRYSSFKDKIDDLRIEPARNLGKRVHDHVESSHFLASFEHWEDTNMSANFASFADDVRPMAQTLPQILFHEEQIFEQLYKHISKHDEYSLQPLLDLLAQFCHDLGPDFMKFYERAMHMLTTLLDDAANFESSNVFEWGFNCLAYVYKYLSRVLAQDLLPTYNLLFPLVSHRKEYLSRFSAEALSFLVRKAKLKSLSAFVTHAFNQLRDVTESTEENACENNIYDGLKMLFTEALITTKESLHSKFNVIMEALVHECLSGAGDQCCISLVADVTMNVLRHASSDNAPPVYDVFISEVKGFLEDPSAKLDAPMKLLLTLAFAESGKKVKSWPELIDSVQKVLCHANVRSLSPEVTALAFCSILRNAPIPDLTKFHRTLFDFYLNNFPQNFIEFFKMGLSMDKDRMFSFNGAKSLQRYIDQNWEFNEKKIALFLMESRQNSPLGQKLALTIPKQFAESIAHGISAESEHLRLGSLFQILWKMQVLYYTSYDASTVIEPLIKTLLQQERLNDFEKDVLGNMLLLLNPRDNGNVLEILDFAVTNLGKFQDSVLCIKGLRQLLGKLENGSNKFSQACENQAFIQISGNLCLPDEQIRYESLKLLISLLKYQGLEVPEILNDCKIIEEIPRNLQTARDITMRIRKLGSDFAKMEPESLVSHVVFNYLFGILTVRFSPVWEGAYEVLCDVYEKNHELVWSLFSKFVEVLDNNFRLEYYEKSQLEEERAVFWSVSTSRLNDVLQTCADVFNSYSFAESSILELLKNRRGDLTYPGLIRNQALKGLLRIPQLAERHSRDIVPYMLKTDPSEENALALEGVEERPSSSSTWSEADRKLLLQLIGKFKNIKSIYKSEEVYQRFMDLLGSRTTEIQKLALDGILAYKESVVIKYRDNLRNLLDDNAFKDESLKLLSHSNDRIVEDNDETFLMPLVLRILFGRAQTPVTSGLKKSRKTAVITLLPSLHEKYVIGFLRLASEGLPYKKFLEQGTGFDSTILSKHLLRRMAGFAALGNLAIKSLGSKYPNATVALLDPALYTACVSNEVASKKSEEEVVLKQAGNVRQAIMKLILNLFSTVGTLIDWDSSVQKIHKLVLQPRISNFEYDNLQHPSSLMSLFCLWATDTCFYKFLYHDECSVAKALMKLLGNSNAKEIVIENTLNFSNYIIKNPTKDDAYVDLVSLVASTCLRELPGLLKNAKSQEVVSITVDLLLNLVEAGYVDEDETKKYLLSSLAHILEGEVKGVQTADKVKVLQSIASLIIGYNCEWTEIEHLYRSCSKLYRVFAEKSLRLSLNKVFISIGTQFEHLSKVAGLLSELNAYSTDRMESYNFKSVLPAFKKIADGSCQLTDELEWLPVIHTCLYFITDEEELAIRTNATHTIKLLIDYSNSKSSASDAEKAVELIGSDLIPHIKNGLRHKNIEIQVEYISVVSYIIANSIYYTELNDMKSLLFKGDEEANFFTNLTHIQLHRRQRAIKRLGENASTLSGSSISHYLIPMVERYIYCTDEKYRNICNETISTVGLLTHFVTWNQYKAVIRRFTAALKQKPAFLKEMVSLIVQCSKSLMKSMQAARAQDPSNISLKKFPKTLTEPENFIQGEIYPTFKNILNTRNEDTIVARIPLCEATVNFILGLDPNDRGRLLPGALSSICQVLRSRSEELREAVRKSLASISVVLGPEYLTFILNELKSALRRGSQIHILSYTVHSLLVAMSHSLSHKDLDTNSALIVSIIMEDIFGAAGQEKEAEGYSSKLKELKFNKSYDTGEILASNISLPAFGSLLHPIRALLSENLGLKNRRKLDELMRRYALGLNHNEESSSKDALILCYEIFTQSANFTSKGGSFKPKASNKLKARDDSFFIVNLNAKDGRVQTETNPNGSVLQKFSLDLLKAVLARNPNLLEAPYLEGFIPLLQGSLDCDDESVLVSALRVLTVFVKLNFDEDSEGIFKNCARKVLNIIKDSPSTSTEICQVSLKFLSSLIRHKDVKLKDTALSFILERVRPDLNEPNKQGLAFNFLKSLLAKHVVLPEMYDIVDSVAGIMVTNHSKEIRDVSRSVYYQFIMEYDQSRGRLEKQFKFLVSNLEYPSQEGRQSVMELINLIVNKSSQDLLRRLSASFFLSLSNVAVNDNSPRCREMASALLRNLLAKLGQGNIGTEEKYILAWLKQDTASFLELGLRIYKIYLSSMSLGANSELDGLALSKARKIIAESDAGSETEWNLVYTALNVLTAYVDATDEAFAVSNTPMWTSVINCLLYPHPWVRLISSRMVNKYVGHQGKLENPFSDLEIQNIAYRIFRQLGAPSISESQATTSIRTIITIMARWQKDNTPFVTAGEGDRKYNTAIDFALSRIGSIIRDEENGRELFPSKKACVQLLALIIQLLDEEQLKHGANTIILSLFTYLEDERRNLDERSEELKQLAQECLELLQGKLSVADFTTIYSSVKHEVTRRRYERRAKRATLAVVAPEAAARRKLKKHARSKEKRKHEKDENGYYHAKNKKRKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNM5</accession>
    <name>C5DNM5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">KLTH0G18348p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0G18348g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the WD repeat SPT8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555823.1">
        <property type="nucleotide sequence ID" value="XM_002555777.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNM5"/>
    <dbReference type="FunCoup" id="C5DNM5">
        <property type="interactions" value="486"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNM5"/>
    <dbReference type="GeneID" id="8294118"/>
    <dbReference type="KEGG" id="lth:KLTH0G18348g"/>
    <dbReference type="eggNOG" id="ENOG502QS8F">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010934_2_1_1"/>
    <dbReference type="InParanoid" id="C5DNM5"/>
    <dbReference type="OMA" id="WDRRQPN"/>
    <dbReference type="OrthoDB" id="10260946at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010557">
        <property type="term" value="P:positive regulation of macromolecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000925">
        <property type="entry name" value="Transcription factor SPT8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057544">
        <property type="entry name" value="Beta-prop_SPT8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848:SF8">
        <property type="entry name" value="F-BOX AND WD REPEAT DOMAIN CONTAINING 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23798">
        <property type="entry name" value="Beta-prop_SPT8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3 9" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Transcription factor spt8 beta-propeller" evidence="11">
        <location>
            <begin position="163"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="299"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="367"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="15"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="38"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="127"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="367"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="384"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="409"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF23798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR25386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="601" mass="67192" checksum="5378398B42F63BDB" modified="2009-07-28" version="1">MDGVGDILNENHVAEEEEEEDEELDEEMEDIAGRGDEQEQDQEDDEQDDQDDQDDQDDQDDQDDDLEQSQDPNQDEDEDMDAEDDENDENDDNDENPNEEDTQDAAGNGARKDTEGARDGNGTLDGRSSEIAKEKSSRGPSAFEKIHQYYTQLHNSAKLADVYSIYPTAAIPIQTHVHSMAMSKGLKYLFLGGQDGYVRKFDFLNTIEGKLSLTILQKHSLVESISNAGILLSYWENEVPQQRNSLKLSKTGKEYEPAVSPVHAIDVQSECLFLLSGLENGGITMQGCRYMEGHIAHYFQKHTSTVNQLKINNDESKFISGGWDKQILEWDLNSGQCVNEFRGSTAQISSLEFRPLYSTVVIDSATNKEDSAGGEDSQIKRDDDDLDSLFGDEEEEEKRRAQESAQEPARPEDGPKSQPLSKDEISQTTLRTVYDENTFLASCTNGSVQVWDRRISTKPALNLIRGNQVPPWCMSACWSTDGDRIYAGRRNAVVEEYDLKMGLNPSKTLKFPSISGPVSCVRAMPNNKHVLCASYDNVRIYDVSQYGSSKVPFLIVPGHHGGMISNLYVDPTCRFLISTSGDRGWQGISTDATLIYDIDVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5E1X5</accession>
    <name>C5E1X5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="20">KLTH0H00418p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="ordered locus">KLTH0H00418g</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S8 family. Furin subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555898.1">
        <property type="nucleotide sequence ID" value="XM_002555852.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E1X5"/>
    <dbReference type="FunCoup" id="C5E1X5">
        <property type="interactions" value="176"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E1X5"/>
    <dbReference type="MEROPS" id="S08.070"/>
    <dbReference type="GeneID" id="8294209"/>
    <dbReference type="KEGG" id="lth:KLTH0H00418g"/>
    <dbReference type="eggNOG" id="KOG3525">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002976_2_1_1"/>
    <dbReference type="InParanoid" id="C5E1X5"/>
    <dbReference type="OMA" id="AYEFDII"/>
    <dbReference type="OrthoDB" id="300641at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007323">
        <property type="term" value="P:peptide pheromone maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04059">
        <property type="entry name" value="Peptidases_S8_Protein_convertases_Kexins_Furin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000026">
        <property type="entry name" value="proprotein convertase subtilisin/kexin type 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000005">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034182">
        <property type="entry name" value="Kexin/furin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002884">
        <property type="entry name" value="P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42884:SF14">
        <property type="entry name" value="NEUROENDOCRINE CONVERTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42884">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01483">
        <property type="entry name" value="P_proprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51829">
        <property type="entry name" value="P_HOMO_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 15" id="KW-0645">Protease</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 15" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5 18" id="KW-0732">Signal</keyword>
    <keyword evidence="4 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950715" evidence="18">
        <location>
            <begin position="23"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="673"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="domain" description="P/Homo B" evidence="19">
        <location>
            <begin position="454"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="598"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="755"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="606"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="630"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="788"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="14 15">
        <location>
            <position position="169"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="14 15">
        <location>
            <position position="207"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="14 15">
        <location>
            <position position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CAR30036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="811" mass="89616" checksum="3618D128B7C74DD5" modified="2009-07-28" version="1">MRLAFCKYFLLQVLLLSIVSQAVRVPVKDHSSRSYFAVESVLEVPRLLKLHPNWVFEHEARGLDKHYVFSTKKDEYSKRDEVEASYEGVKSYHPLPPKKLQKRLPVPDRPVDSSLEVVNEVRDRLSIEDPLFGIQWHLVNGNYPGHDVNVSGLWYENVTGHNVVVAVVDDGLDYESEDLKDNFSAEGSWDFNDNGPMPKPRLSDDYHGTRCAGEIAAVKNKACGIGVAYNAKVAGIRILSAEVTAEDEAASLIHALDVNDIYSCSWGPLDDGRVLQGPDDLVRKALVTGVTKGRNEKGALYVFASGNGGMYDDNCNYDGYTNSIYSITVGAIDHKGLHPPYSESCSAVMVVTYSSGSGEHIHSTDIHGKCSDTHGGTSAAAPLAAGVYSLVLEANPNLSWRDVQYLSILSSEEINDDDGEWQEGALGKRYSHKYGYGKLDAYRIVTMGREWENVGPQSWYYSSVQKVGKSTNSTDEDLVSSISVSSEQLKNANFKRVEHITVTVSIETTIRGRTTINLVSPKGIVSKLGVVRRSDTSPEGFQNWTFMSVAHWGEIGEGDWSLHVRTTEDSNNVEFKDWRLKLFGESLDASKAKTFEFGNDKEPTEDLQQQQQEESVSDHPTSLASSTQPTFTAASSITSTHSTVSATTTTAETSDQDVNSPNKLPRPFEGLRYFLAIFAVGFLVLVLYYYFFVKSRRLVRRSRAEAYEFDIIDTDSDHDSSLNSASNTSVPMMGELNEEDFNFDLSDEELLRSATVSPAPRYEQNVPAGASSDDLPTADPSKGKVHSENSSNNNGKRNNMPREGSQGPNNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5E241</accession>
    <name>C5E241_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Translation initiation factor eIF2 assembly protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0H01958g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the CDC123 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555964.1">
        <property type="nucleotide sequence ID" value="XM_002555918.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E241"/>
    <dbReference type="FunCoup" id="C5E241">
        <property type="interactions" value="799"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E241"/>
    <dbReference type="GeneID" id="8294277"/>
    <dbReference type="KEGG" id="lth:KLTH0H01958g"/>
    <dbReference type="eggNOG" id="KOG2983">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034402_2_0_1"/>
    <dbReference type="InParanoid" id="C5E241"/>
    <dbReference type="OMA" id="TFPDPNF"/>
    <dbReference type="OrthoDB" id="360540at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009772">
        <property type="entry name" value="CDC123"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15323:SF6">
        <property type="entry name" value="CELL DIVISION CYCLE PROTEIN 123 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15323">
        <property type="entry name" value="D123 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07065">
        <property type="entry name" value="D123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007807">
        <property type="entry name" value="Cdc123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="346"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR007807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR30102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="366" mass="42125" checksum="7A53488AC81C13F6" modified="2009-07-28" version="1">MTNEYVELSEIKVSVQQVKNCAFSQWYSKFAGHTPQAKVIKPVPGGFLEYLEQDGIKLPNDSQFGKSSYYAEIGNNEDNEYSDWEASSDEEHPQGQDVVQLFPELHKELKDIFREMGSLTPKLTWSSPKDATWILANNTLKCTEVNDMYLLLKASNYITHDLDRALAECFDKNSALEQDGPLQHELVLRKWFDLNPALEFRVFVKDSAIIGVSQRDLNYYDYLELLTETFKDLLDEFVEDIVLPRFPDSSFVCDVYIPRPFEKVWLIDFNPFARKTDSLLFSWNELATIDPYKLESDYELRLVQENNVARFASKEHSENQVPKEVADAITNPKAIQELAEKWGELLNMQKAGDSSDDEPGEQNNSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2J2</accession>
    <name>C5E2J2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0H05434p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0H05434g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bystin family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556115.1">
        <property type="nucleotide sequence ID" value="XM_002556069.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2J2"/>
    <dbReference type="FunCoup" id="C5E2J2">
        <property type="interactions" value="936"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2J2"/>
    <dbReference type="GeneID" id="8294430"/>
    <dbReference type="KEGG" id="lth:KLTH0H05434g"/>
    <dbReference type="eggNOG" id="KOG3871">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029727_0_1_1"/>
    <dbReference type="InParanoid" id="C5E2J2"/>
    <dbReference type="OMA" id="TKLPVIW"/>
    <dbReference type="OrthoDB" id="2192561at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007955">
        <property type="entry name" value="Bystin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12821">
        <property type="entry name" value="BYSTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12821:SF0">
        <property type="entry name" value="BYSTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05291">
        <property type="entry name" value="Bystin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="39"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="90"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR30253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="462" mass="53335" checksum="035853D9A7CFBD86" modified="2009-07-28" version="1">MGRVASSKSRKQRHDPLLKDLDSAQGNLRKAPKKREDQKDEDDQEEYVDAASSRRILQLAKEQQQELEEEQENEKNGGNNFARFRLAGNYEEDEEEDEDQEREENISDFEPEYDEEQEEEEVVEIDEEDAAMFEQYFKRSTDYNSDFQSYNLADKIMASIREKEMELNAGSASARDETQPEGVALPPKVIRAYSTVGVILKTWTHGKLPKLFKVIPSLNNWQDVLYVTNPADWSPHVVYEATKLFVSNLPAKEAQKFINAVLLDRFRDNIETAEDHSLNYHIYRALKKSLYKPSAYFKGFLFPLLEMGCNIREATIAGSVLAKVSVPALHSAAALSYLLRLPFSPATTVFIKILLEKKYALPYQTVDDCVYYFMRFRILSDGSNGEDSSRTLPVVWHKAFLSFAQRYKNDITQDQRDFLLETVRQRGHKDIGPEIRRELMAGESREFVNNLESKDDLMLDVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2J9</accession>
    <name>C5E2J9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glutathione peroxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0H05588g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a hydroperoxide + [thioredoxin]-dithiol = an alcohol + [thioredoxin]-disulfide + H2O</text>
            <dbReference type="Rhea" id="RHEA:62620"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:35924"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.11.1.24"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the glutathione peroxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556122.1">
        <property type="nucleotide sequence ID" value="XM_002556076.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2J9"/>
    <dbReference type="FunCoup" id="C5E2J9">
        <property type="interactions" value="497"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2J9"/>
    <dbReference type="PeroxiBase" id="12379">
        <property type="entry name" value="LtheGPx01"/>
    </dbReference>
    <dbReference type="GeneID" id="8294437"/>
    <dbReference type="KEGG" id="lth:KLTH0H05588g"/>
    <dbReference type="eggNOG" id="KOG1651">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029507_2_2_1"/>
    <dbReference type="InParanoid" id="C5E2J9"/>
    <dbReference type="OMA" id="TFPMTEK"/>
    <dbReference type="OrthoDB" id="446890at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004602">
        <property type="term" value="F:glutathione peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047066">
        <property type="term" value="F:phospholipid-hydroperoxide glutathione peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140824">
        <property type="term" value="F:thioredoxin-dependent peroxiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00340">
        <property type="entry name" value="GSH_Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000010">
        <property type="entry name" value="Glutathione peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000889">
        <property type="entry name" value="Glutathione_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029759">
        <property type="entry name" value="GPX_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029760">
        <property type="entry name" value="GPX_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11592">
        <property type="entry name" value="GLUTATHIONE PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11592:SF78">
        <property type="entry name" value="GLUTATHIONE PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00255">
        <property type="entry name" value="GSHPx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000303">
        <property type="entry name" value="Glutathion_perox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01011">
        <property type="entry name" value="GLUTPROXDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00460">
        <property type="entry name" value="GLUTATHIONE_PEROXID_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00763">
        <property type="entry name" value="GLUTATHIONE_PEROXID_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51355">
        <property type="entry name" value="GLUTATHIONE_PEROXID_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2 9" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="6" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="8">
        <location>
            <position position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000303-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR30260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="161" mass="18413" checksum="62F8CCB3B81A055F" modified="2009-07-28" version="1">MSKFYDLAPLDRTGKPFPFKQLEGKVVLIVNVASKCGFTPQYEELQELYKRYHDKGFEIIGFPCNQFGHQESGTDEEIGQFCKLNYGVTFPVMKKVHVNGDNADPVYEYLKNEKAGMLGFKGIKWNFEKFLIDKHGNVYERYSSLTKPANLDAKVQKLLGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2K9</accession>
    <name>C5E2K9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0H05808p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0H05808g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556132.1">
        <property type="nucleotide sequence ID" value="XM_002556086.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2K9"/>
    <dbReference type="FunCoup" id="C5E2K9">
        <property type="interactions" value="64"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2K9"/>
    <dbReference type="GeneID" id="8294447"/>
    <dbReference type="KEGG" id="lth:KLTH0H05808g"/>
    <dbReference type="eggNOG" id="ENOG502QUSW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019840_0_0_1"/>
    <dbReference type="InParanoid" id="C5E2K9"/>
    <dbReference type="OMA" id="EMKYGYL"/>
    <dbReference type="OrthoDB" id="4081443at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="104"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="112"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR30270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="647" mass="74161" checksum="9EE46DFCD5083F48" modified="2009-07-28" version="1">MLRFVQQSHVLKSRVSNQFVSASARSPFHHSLRFNSAVAMDRTPSQEAAAVAPAPPNVAKAVDKVRAMRKNIKINKQLSATPDSPWFHKVCAFEDCVAQTLYMSQTPRRRNNNNNNNGRRGQQQPLAASNTNPLFWDSIGRAMGLYRELLGTPELNSDRVSKLVHLLHNGLRANRNQLTRMNKKPDYDSQSFHKEMTNYLCESLREISQDVLSAKVELNEYGAMHLITAFKELLLFEEAVSIWRSAINGNSNYTANIFLNPRVVGVVLPILYDNGVTYAEIQELYEKSSAMINYFHPNLSVGMIRASLSAGENEMALTLFQKLCEESSEMKYGYLIETHLSFIGECKDLNISQAFFDKALNNEMPYKIDLQVSYVKSFLKNIWSQTHDFQKVYDIWYKSSVHYGRDVNHGISSSLNDTFFDIFFEHYSQDKIIGLQQLQEMISIYNNIKPIDEPFFNIILTKCTVWRDRQIIESIDKAYDLYQIPRTIVAYRILLKSMGSVHVTNEEIFQRWCNLVKKSDEIGQSFIANADWAALRDATVTWTQLYSQPDDLYSDGFNAAMQAANASGAFDDIPQQTQQQQPNDRLELYMKIVKRYSPFCRDSRQLSRLTSGTAVKYPVLQQALSKFNDLDVSDVYIPQFHNLVAQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2P4</accession>
    <name>C5E2P4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">DASH complex subunit DUO1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Outer kinetochore protein DUO1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ordered locus">KLTH0H06622g</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
            <location evidence="3">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DASH complex DUO1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556167.1">
        <property type="nucleotide sequence ID" value="XM_002556121.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2P4"/>
    <dbReference type="FunCoup" id="C5E2P4">
        <property type="interactions" value="84"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2P4"/>
    <dbReference type="GeneID" id="8294483"/>
    <dbReference type="KEGG" id="lth:KLTH0H06622g"/>
    <dbReference type="eggNOG" id="ENOG502S4KM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114619_0_0_1"/>
    <dbReference type="InParanoid" id="C5E2P4"/>
    <dbReference type="OMA" id="DTWIKIQ"/>
    <dbReference type="OrthoDB" id="4067138at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042729">
        <property type="term" value="C:DASH complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013960">
        <property type="entry name" value="DASH_Duo1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28216">
        <property type="entry name" value="DASH COMPLEX SUBUNIT DUO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28216:SF1">
        <property type="entry name" value="DASH COMPLEX SUBUNIT DUO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08651">
        <property type="entry name" value="DASH_Duo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="16" id="KW-0137">Centromere</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="10" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="8" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9" id="KW-0498">Mitosis</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="156"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="156"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00044152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00044358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CAR30305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="223" mass="24911" checksum="A67D02B2AC47A6A4" modified="2009-07-28" version="1">MSEHAEEDSNINKLIPEIFDQMRSNLSLAPLAGGIKPFVSSKSSSNLTTQSLLEENKCLDKIIPVIQKLNSSLSSSGPQHLKRIKETCESSNKILDAWIKIQSQAGYVHELMSDHSYLEYVSATQENEALTAEAFVQRKEAQVASLKRTLEEKQQLRESVKKEQEVRSSSDVPSLPRGRRAINPRREVRGGMKKPTGIPRSSTRVSKPPLSRPPHSSVKKPSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2Y2</accession>
    <name>C5E2Y2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0H08756p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0H08756g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556255.1">
        <property type="nucleotide sequence ID" value="XM_002556209.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2Y2"/>
    <dbReference type="FunCoup" id="C5E2Y2">
        <property type="interactions" value="75"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2Y2"/>
    <dbReference type="GeneID" id="8294579"/>
    <dbReference type="KEGG" id="lth:KLTH0H08756g"/>
    <dbReference type="eggNOG" id="KOG4113">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_124782_0_0_1"/>
    <dbReference type="InParanoid" id="C5E2Y2"/>
    <dbReference type="OMA" id="GPIGMVC"/>
    <dbReference type="OrthoDB" id="30840at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006892">
        <property type="term" value="P:post-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.10">
        <property type="entry name" value="Metal Binding Protein, Guanine Nucleotide Exchange Factor, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007515">
        <property type="entry name" value="Mss4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011323">
        <property type="entry name" value="Mss4/transl-control_tumour"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13276">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR MSS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13276:SF0">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR MSS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04421">
        <property type="entry name" value="Mss4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="Mss4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51796">
        <property type="entry name" value="MSS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR30393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="135" mass="14923" checksum="42FF25489E547850" modified="2009-07-28" version="1">MTKLRCPFAGCDSCIVSAEEGRKVVLPAEVYDKLRLALPSNQAPRSGEFSVFEDIWDFDNIGVSRQVPTEIEAAAENAQELRFTWKERPYTLGKLQKYLICADCDRGPLGIVCEILDAEGARETVNLLSLASVLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5E345</accession>
    <name>C5E345_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0H10296p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0H10296g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556318.1">
        <property type="nucleotide sequence ID" value="XM_002556272.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5E345">
        <property type="interactions" value="117"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E345"/>
    <dbReference type="GeneID" id="8294649"/>
    <dbReference type="KEGG" id="lth:KLTH0H10296g"/>
    <dbReference type="eggNOG" id="ENOG502QPT7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_241900_0_0_1"/>
    <dbReference type="InParanoid" id="C5E345"/>
    <dbReference type="OMA" id="MKLEYYT"/>
    <dbReference type="OrthoDB" id="4070686at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022031">
        <property type="entry name" value="Rif1_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12231">
        <property type="entry name" value="Rif1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Telomere-associated protein Rif1 N-terminal" evidence="2">
        <location>
            <begin position="239"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1231"/>
            <end position="1256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1318"/>
            <end position="1388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1409"/>
            <end position="1432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1488"/>
            <end position="1515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="55"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="112"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1232"/>
            <end position="1241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1323"/>
            <end position="1337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1365"/>
            <end position="1388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1420"/>
            <end position="1432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1499"/>
            <end position="1508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR30456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1668" mass="188337" checksum="A7C5B7B3516D5AD1" modified="2009-07-28" version="1">MTANSSPTKKRGKALDVLEKHISQQNKKPKNNQYGALLSMFQTITDSIKLPAPVSQPRPRMISSSPPRRVEQPFGSRQHPTSKSPEPPSQRSSPKKSVAFSDQIDSSPPPASLHSSPHRSSQAVPSKPILKFTLPLARTPSPRRKNNQPGFNDKLTIDQRENDSSEDPMSLSYWVSGEVHGLGDIKSISEFKDVLTGGLYFLSSDIPEAKKRYFEIYATFNNIMPIFTSPNYSEVKDKKVQILAQNMNVILATCIPHLISIQAELLSSLKKDPFTSRIYVQIVRFFNALFSNFKLVKICENNQSFQTALHKVVESFTEAIQHPNSNKVMVTAQLALLRDEQCGIKRLTPLKVQSFVKSLANMKKISSSNLTCEKLLLLKIFLAKYPEVMFETIDIWLQAEVLSRLLIEEEFYSLKIANNCVSILLDLLKRCLTDRDNHQVLEVLKQTTTKELFSVNCEGNKTLAHLQTKEDNLETLLRKRVIYLIEEKDEPKLAMDLWLAMVGLLFNSAGEVSSLCSDRGKLWLDLNLRYQNNSNPKAKKLALRSWRIVTYMICSKRLADIGESQKLVAVLLDPFSYLSKDYYKDPDYESLIYVLNGLLYSTLCNHHKDQFTYNLEFILSPFINEILMGQKSLKIRKVALEVLLRVVNQSPGGPTPKKEFNALKVVTSSGVEAADFIPFSSPLLSRNWHALMSIVQKCKQTSPPETYDLSFTLLLAVIERIPQRAIDGTTRDNCSATLLPGTEDSEQKPRSRMTDALASLMRTFKDLMCEPSPIINKLCMEIATAIKNGEIDCTDMLKTVLKETSNSISYLEVYSFFLRLKDTRIDDFIYNSISSKILSPKISAHAFEAFIDIVNSYSSTEIIQAFLDTSEKGSFDLWKASKLDTSRWDFGCTVRYFTMRFQREDLKVDDEFVSQFSSALTQHSRLAEELRKSLGVDRYAKSVVKFLKQEKRTDSYGLDSQWKLSMISCFSMDDYLEMLKFAADLREDVQLLLINGAQNNDESLHLSLRFIQLLISSEKQIVDDIKNGCDRALYELLEKCYQKKLLCLLNQCVLFIIENDAYYLVEKFWATCGPDFLNLLENSTLATIMNQVASKNEVVINIFNFVFEQKPTDYVLGLMENVLAQDQLPTLNCCREKIINFLVREGIRSNEGGRKKVLAIFERASEVLIFQHKRLLAEFMRSLISKLPAEKGEFVYDLLSFICKDERFKLKGYDNLLVKINHGLPLASDTFQSEKKRETQRYKFHSAPQTPPNKTSSVSEAAAYSLNQLPQKVVEMRETSPSDATFPSCRELSSNLNQQRKLKESAAASETKTLHALGTVLNDQKHMDTSDEDQSCREKKRIPGPNNGDKALRSITPPEETVCSSKDDGLTPRNNEKGGQDPTHKLTEISQKKQIIVSEVAQSKLQASRGAEALSEKSTTKGQIKQNVKSQGVSLNDEVETVAVPSPPKKHESSTTDAAAQIACSKLHENAQKTHHGDEVHEKRSCFEETQPKAASASALHNENSQISDGEKRTSVDSKVGSLVIEQSLMNETLMNREGFSRGTDLSNAIGIKSTFSNVESLAKIPIFNSRKLISKNKQPVTCENKSNEGVTANKSSKELPGKLEDFETIKRKNLHGSVYSACEDKKLKVILQIMKEFGVNEFSQLSDVQKQFVKQEMIKFLSKSNAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3D3</accession>
    <name>C5E3D3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 18">CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase</fullName>
            <ecNumber evidence="5 18">2.7.8.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ordered locus">KLTH0H12430g</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Essential for the viability of mitochondrial petite mutant. Catalyzes the committed step to the synthesis of the acidic phospholipids.</text>
    </comment>
    <comment type="function">
        <text evidence="18">Functions in the biosynthesis of the anionic phospholipids phosphatidylglycerol and cardiolipin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 18">
            <text>a CDP-1,2-diacyl-sn-glycerol + sn-glycerol 3-phosphate = a 1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycero-3'-phosphate) + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12593"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:58332"/>
            <dbReference type="ChEBI" id="CHEBI:60110"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="2.7.8.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 18">Phospholipid metabolism; phosphatidylglycerol biosynthesis; phosphatidylglycerol from CDP-diacylglycerol: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 18">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 18">Belongs to the CDP-alcohol phosphatidyltransferase class-II family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.5" evidence="5 18"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556406.1">
        <property type="nucleotide sequence ID" value="XM_002556360.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3D3"/>
    <dbReference type="FunCoup" id="C5E3D3">
        <property type="interactions" value="448"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3D3"/>
    <dbReference type="GeneID" id="8294745"/>
    <dbReference type="KEGG" id="lth:KLTH0H12430g"/>
    <dbReference type="eggNOG" id="KOG3964">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030471_1_1_1"/>
    <dbReference type="InParanoid" id="C5E3D3"/>
    <dbReference type="OMA" id="HKCLAQC"/>
    <dbReference type="OrthoDB" id="10250191at2759"/>
    <dbReference type="UniPathway" id="UPA00084">
        <property type="reaction ID" value="UER00503"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008444">
        <property type="term" value="F:CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032049">
        <property type="term" value="P:cardiolipin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09135">
        <property type="entry name" value="PLDc_PGS1_euk_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09137">
        <property type="entry name" value="PLDc_PGS1_euk_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.870.10:FF:000044">
        <property type="entry name" value="CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.870.10:FF:000046">
        <property type="entry name" value="CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016270">
        <property type="entry name" value="PGS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025202">
        <property type="entry name" value="PLD-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12586:SF1">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--GLYCEROL-3-PHOSPHATE 3-PHOSPHATIDYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12586">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--SERINE O-PHOSPHATIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13091">
        <property type="entry name" value="PLDc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000850">
        <property type="entry name" value="Phospholipase_D_PSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00155">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 18" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="12 18" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13 18" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14 18" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="15 18" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 18" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PLD phosphodiesterase" evidence="19">
        <location>
            <begin position="178"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00014944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00058636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU365024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CAR30544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="523" mass="59645" checksum="CFC979FFE5DC45AD" modified="2009-07-28" version="1">MRPATLQKQGIFAGSFWRSLRLHTTSVESMKNSQKAAQSRRVSYTDEVKQQLSKLNACFFFRKGEIKILDTPSCFYNTLKQKISVAKDRIFLASLYIGTSERELIKCISDALDRNASLKVYFLVDGLRGTREAPKSCSASLLSELLRSHKDRVDIRLYRTPDLTPFKEAVIPRRFNEGIGLQHMKIYGVDDEVILSGANLSNDYFSNRQDRYYLFKSRKLASYYFKLHQLVSSMSYQVHYSEAVQKYKMIWPHDNIAVDPRADKASFLKVCSSTLKTFLTDPVDETADYEAFEPKTYSTRVYPISQFTPLFRASPQDYSTEKPTILQILSHIPDGSVKWTFTAGYFNMLPEIKSLLLGAPCKEGNVITASPFANGFYQSKGVSKFLPSAYLHLSEKFLSEVRNRGKESQISLNEWKKGIVNTPGGWSYHAKGIWLSDNNEKDQRPAITIVGSSNYTRRAYSVDLESNVVLVTEDSDLKDEMQAEIDRLMFHTEPVTIENFKNEPQRHVSLGIQAATRIIGKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3E8</accession>
    <name>C5E3E8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">1-phosphatidylinositol-3-phosphate 5-kinase</fullName>
            <ecNumber evidence="2">2.7.1.150</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Type III PIP kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">KLTH0H12804g</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,5-bisphosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13609"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57923"/>
            <dbReference type="ChEBI" id="CHEBI:58088"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.150"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.1.150" evidence="2"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556421.1">
        <property type="nucleotide sequence ID" value="XM_002556375.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5E3E8">
        <property type="interactions" value="896"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3E8"/>
    <dbReference type="GeneID" id="8294762"/>
    <dbReference type="KEGG" id="lth:KLTH0H12804g"/>
    <dbReference type="eggNOG" id="KOG0230">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000480_3_1_1"/>
    <dbReference type="InParanoid" id="C5E3E8"/>
    <dbReference type="OMA" id="QSVWNDT"/>
    <dbReference type="OrthoDB" id="158357at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000285">
        <property type="term" value="F:1-phosphatidylinositol-3-phosphate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03334">
        <property type="entry name" value="Fab1_TCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17300">
        <property type="entry name" value="PIPKc_PIKfyve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.810.10:FF:000001">
        <property type="entry name" value="1-phosphatidylinositol 3-phosphate 5-kinase FAB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000007">
        <property type="entry name" value="1-phosphatidylinositol 3-phosphate 5-kinase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000283">
        <property type="entry name" value="1-phosphatidylinositol-3-phosphate 5-kinase (Fab1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.800.10:FF:000005">
        <property type="entry name" value="1-phosphatidylinositol-3-phosphate 5-kinase (Fab1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.810.10">
        <property type="entry name" value="2-Layer Sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.800.10">
        <property type="entry name" value="Phosphatidylinositol Phosphate Kinase II Beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044769">
        <property type="entry name" value="PIKfyve_PIPKc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027483">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002498">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027484">
        <property type="entry name" value="PInositol-4-P-5-kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45748">
        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL 3-PHOSPHATE 5-KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45748:SF7">
        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL 3-PHOSPHATE 5-KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01504">
        <property type="entry name" value="PIP5K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00330">
        <property type="entry name" value="PIPKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51455">
        <property type="entry name" value="PIPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FYVE-type" evidence="15">
        <location>
            <begin position="209"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="PIPK" evidence="16">
        <location>
            <begin position="1789"/>
            <end position="2119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="76"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="269"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="323"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="464"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1638"/>
            <end position="1662"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1748"/>
            <end position="1783"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1795"/>
            <end position="1840"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="952"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="1270"/>
            <end position="1297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="13"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="76"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="269"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="349"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="472"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1753"/>
            <end position="1764"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1765"/>
            <end position="1776"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1795"/>
            <end position="1809"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1811"/>
            <end position="1821"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00075294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAR30559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="2132" mass="240859" checksum="C6D2BBD9DD18B2CC" modified="2009-07-28" version="1">MGSAGMEAPEFLSQKSLKPKTTTDLDTNPVLSDISLPAKLEVSNTDEHESTSTNNRPPHTLESCEDFHGLEPVLHESQNGVSPRNSKGMASKVHSEGTPSNRKSMGLELHSGIGNSNQQLRPYSIPLASSARTKRSSIYHMKTPNAIPIRAPSHRSTHFEGDDRTSVKSISSSLTASFSKSFLFGFYHNYKNGSKASKSVLSKEYWMKDESAKDCFSCAKPFTTFRRKHHCRICGQIFCGNCTFLIDGGKFQHDGKMRVCRNCHTDVDTLEDSSDESSAEEHEESTNEDQVESSLDQRLPSTDVLILEDDEVQSILADKKGAQVFRSTPPPPPKMAIPATRQGGSLEISVPSRSSPSSRGDASRHWRSMRDVYTIRDVDILPPYTIDTKGSRGSHGHVSALSSQAHEAHIPHLKHSMSSFAPNGGNNEVYKQPSRSPSSVIANLSNNDFKFEFNYRTKNFQDYLNGNNSRNSRTEQTHINRSESSPILPSHQEDTIIDNESGISDDGSEDERSMSLYAALNDSHQDQHYMRPMRNTTKSFQRAQASLQRMKSRGKFKGRSHASNSVVRKQFEFFNQSSPFLKPALNNDEDYFNLNESSLCLERPHPRNDASTGSRDMRRIISTASVLRLKSQESKSELSDVAQLHMNALLNQVLKDQDVEDLNEWIEVFKSILSSLQGIELDARNMNGLDFKQYYIKIKRIGGSFISNSRFVNGVVYSKNIPLKSMPRCLTNPRILLIMFPLEYQKNENHFMSLDSIMAQEEEYLRKLVLRLKSLNPDVVLVGANVSGFALKLMNDAGILVQFNMKPQVMERIAKLTEASIAITVDKLATSVKLGTCASFQVRTFCYGNLVKSYTFLEGCKASLGGTILLRGSSFDVLQKIKDITEFMAYAVFSLKLESSFFNDNFLQLSVELYKQSHKQQLESAAPQYFADFIEKFHQRILSVSPTVEFPMPFLLRKARELESELIRKESESKDLAATSTNSNSDLEKTIQDLGLESALTNQDFKNLVKFVHKKDVEDLKARFKWRSRQWELSYALSKNMLGTGTHQTIHVLYSMISKKTATPCVGPQLVSIDYFWDTDISIGQFIENIVATANCTCLHGCGGILLDHYRSYVHGTGKVDVLIERFQSRLPSLKNILLTWSYCKECSSSTPILQMSKKTWNYSFGKYLELLFWSRGEGIKTIGNCNHDFAKDHVKYFSYDDLMVRMEYSNVDVHELITPRSQISWKPNIDIKLKVELYYQILEKINNFYASVSDRLLRIKLDSMSSDRAVAAEGKIEELKKRVLKEKEILIDLTESIYRNTPGDQHLPLNATIRSLHDNAAGWDSEFSEFGKTFLPSENDIARITAIQLKKLFTDAAKTDQEQITLTEKLTTSDATQQKAHETGSDVTKNEIANDKRLSNMFEGSTTIGKLGSLYQDRSETFKEAHRPSITHRQTTPNIKKINTRDNSAILPKDDILIGSKKKVQGSTSDVRPSLDILTARPPIDLSDSVAKNSNSLRSLSNGSGGSFNSLHSKEYGSGSKVGQLANFFDQIHFDALSKEFELQREFERLQLNKNKYKALRVEPSKPIVEIYKNAKDAVDGPLHTDSRRKKLPFANGRQEWKNITNASKSHCSSNDELENKLEHSIHQWGERILRKEDKSVSDEGSTSENLTEKHESGEALSPVVTPAAAKMHKLDPESQPEKSSLMKTLANFWADRSATLWKSLDYPTASTEHIFVDSPVIIREDEPCSLISFCLSSSDYLQKMRVSNSSEGEKNGSNRAEPHNSTPCGTNAQGISEGGEANESLLSNDTAANKNSSLQNKESSTESAPEPREDTKTDGNDVENSNTGETEETNSEKIMTKKTAMHLRYQFQDGSSVMSCKIFFAEQFDAFRKTCSCEENFIQSLSRCVKWDSSGGKSGSAFLKTLDDRFVIKELSHTELDSFIKFAPSYFEYMAQAMFHDLPTALAKVFGLYQIQTKNSDSGKSFKMDVIIMENLFYDKKTSRIFDLKGSMRNRHVKQTGKENEVLLDENMVEYIYESPIFVREYDKKLLRASLWNDTLFLAKMNVMDYSLVIGVDNNNHTLTVGIIDFIRTFTWDKKLESWVKERGFVGGSTKEPTVVTPRQYKNRFREAMERYILMVPDPWFQEIHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBG5</accession>
    <name>C5DBG5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0A02420p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0A02420g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551564.1">
        <property type="nucleotide sequence ID" value="XM_002551518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBG5"/>
    <dbReference type="FunCoup" id="C5DBG5">
        <property type="interactions" value="25"/>
    </dbReference>
    <dbReference type="GeneID" id="8290361"/>
    <dbReference type="KEGG" id="lth:KLTH0A02420g"/>
    <dbReference type="eggNOG" id="ENOG502S29D">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_726055_0_0_1"/>
    <dbReference type="InParanoid" id="C5DBG5"/>
    <dbReference type="OMA" id="WMSIDEQ"/>
    <dbReference type="OrthoDB" id="3979912at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="238"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="238"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="284"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="393" mass="43014" checksum="13937F4BDA7B23AC" modified="2009-07-28" version="1">MLLERFQSKLHGSRLSRHSNSKSRSKCDADAQPVQNIADDLMTGDGMSDSDAYASMRELQISTQLCEPRSGTPLNGVSTPVMQPIMPYMYGNQKRQDSVASFASSISDMPGPARTATTPLPTTFSTQFVKMLMEVYQDICSDPTATPFDASNPPSGILNRTAKVAVERAEQKGVDMGREKNALLVNSVKQRLLQELRRDAYLSRNSSIVSLPPMPQFNTSDIAPTACVDYFSTHAEASSNGQLSTPFQMPTSSLGSSQPLQTPFDQRPQVPKLSHRSRNDSFISAAGRSRSGSSHMFPSQQQQQQPQPSAALQPSSFIETTTAPRAQFEGNGYFVLTPTNSMLGEPQQPFIARERSNASSAFEDMMSGELSRHRQNSFRIEKGSATGSLAMDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBJ2</accession>
    <name>C5DBJ2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0A03080p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0A03080g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551591.1">
        <property type="nucleotide sequence ID" value="XM_002551545.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBJ2"/>
    <dbReference type="FunCoup" id="C5DBJ2">
        <property type="interactions" value="191"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBJ2"/>
    <dbReference type="GeneID" id="8290391"/>
    <dbReference type="KEGG" id="lth:KLTH0A03080g"/>
    <dbReference type="eggNOG" id="ENOG502S125">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105947_0_0_1"/>
    <dbReference type="InParanoid" id="C5DBJ2"/>
    <dbReference type="OMA" id="KINYEMV"/>
    <dbReference type="OrthoDB" id="4063618at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="138"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="228" mass="25675" checksum="90077113E92D1674" modified="2009-07-28" version="1">MSDESALSICEDSVREFTELLAKDTQIRYAQNAIRRYKSSEAVDVDDERKSRHKRNIIAVDDNLKINYEMVKMTPGTFIESSVSTSKNKALKEKAGKVTKMKTNYYKKRLYNMNEQFLAEQGTLEPVSCSDSQSPESAADAGDAGDTAGAASIADDAPLDQQARWLAEIHKDLLQQYNTLLQEEKKWFLKKEVLLDANAKLDLFTTRDEDQSTLQLGSKDTAGLCMFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBT8</accession>
    <name>C5DBT8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">D-2-hydroxyglutarate dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="11">1.1.99.39</ecNumber>
            <ecNumber evidence="17">1.1.99.40</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">D-lactate ferricytochrome C oxidoreductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ordered locus">KLTH0A05280g</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the oxidation of D-2-hydroxyglutarate (D-2-HG) to alpha-ketoglutarate. Also catalyzes the oxidation of other D-2-hydroxyacids, such as D-malate (D-MAL) and D-lactate (D-LAC). Exhibits high activities towards D-2-HG and D-MAL but a very weak activity towards D-LAC.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>(R)-2-hydroxyglutarate + pyruvate = (R)-lactate + 2-oxoglutarate</text>
            <dbReference type="Rhea" id="RHEA:51608"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15801"/>
            <dbReference type="ChEBI" id="CHEBI:16004"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="EC" id="1.1.99.40"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>(R)-lactate + 2 Fe(III)-[cytochrome c] = 2 Fe(II)-[cytochrome c] + pyruvate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13521"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16004"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.1.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>(R)-malate + A = oxaloacetate + AH2</text>
            <dbReference type="Rhea" id="RHEA:67460"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15588"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:67461"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FAD-binding oxidoreductase/transferase type 4 family.</text>
    </comment>
    <dbReference type="EC" id="1.1.99.39" evidence="11"/>
    <dbReference type="EC" id="1.1.99.40" evidence="17"/>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551687.1">
        <property type="nucleotide sequence ID" value="XM_002551641.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBT8"/>
    <dbReference type="FunCoup" id="C5DBT8">
        <property type="interactions" value="411"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBT8"/>
    <dbReference type="GeneID" id="8290491"/>
    <dbReference type="KEGG" id="lth:KLTH0A05280g"/>
    <dbReference type="eggNOG" id="KOG1232">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017779_4_1_1"/>
    <dbReference type="InParanoid" id="C5DBT8"/>
    <dbReference type="OMA" id="YNEDWMR"/>
    <dbReference type="OrthoDB" id="5332616at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051990">
        <property type="term" value="F:(R)-2-hydroxyglutarate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004458">
        <property type="term" value="F:D-lactate dehydrogenase (cytochrome) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006089">
        <property type="term" value="P:lactate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006108">
        <property type="term" value="P:malate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2190:FF:000001">
        <property type="entry name" value="D-2-hydroxyglutarate dehydrogenase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2740:FF:000002">
        <property type="entry name" value="D-2-hydroxyglutarate dehydrogenase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.43.10:FF:000002">
        <property type="entry name" value="D-2-hydroxyglutarate dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.465.10:FF:000053">
        <property type="entry name" value="D-lactate dehydrogenase (Cytochrome), putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.45.10:FF:000001">
        <property type="entry name" value="D-lactate dehydrogenase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="entry name" value="Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.45.10">
        <property type="entry name" value="Vanillyl-alcohol Oxidase, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004113">
        <property type="entry name" value="FAD-bd_oxidored_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016164">
        <property type="entry name" value="FAD-linked_Oxase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051264">
        <property type="entry name" value="FAD-oxidored/transferase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016171">
        <property type="entry name" value="Vanillyl_alc_oxidase_C-sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43716">
        <property type="entry name" value="D-2-HYDROXYGLUTARATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43716:SF1">
        <property type="entry name" value="D-2-HYDROXYGLUTARATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02913">
        <property type="entry name" value="FAD-oxidase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55103">
        <property type="entry name" value="FAD-linked oxidases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="19">
        <location>
            <begin position="107"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00039639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00050208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00051436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00066713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00083446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CAR21245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="541" mass="60103" checksum="AADAC212DF233B2F" modified="2009-07-28" version="1">MLRSTVRKPLQLASRVKLSTASWSASRRAVPLQAGRVWWPKVQEYSTKIQPKLTAESYPDVHRFSGFKKLADEDLSFFRSILSDSEILQAKADEDLALFNEDWMRKYKGQSQLVLRPKSVNQVSQILKYCNEQKLAVVPQGGNTGLVGGSVPVFDEIVLSLTQLNKIRDFDPVSGILKCDAGVILENADSFLAEHGYIFPMDLGAKGSCHVGGIVATNAGGLRLLRYGSLHGTVLGLEVVLPNGEILDSMNALRKDNTGYDLKQMFIGSEGTIGVITGVSILTPPRPKAFNVSFLGVESYEAVQKTFVQAKQELSEILSAFEFMDLNSQRLTKEHLKDVPHPLEEEYPFYILIETSGSNKEHDDVKLETFLEKAMEDGTVLDGVVAQDETELRNLWQWREMIPESSQAGGGVYKYDVSLPLKDLYSLVEAANAKLTEKGLLSVEDTSKPVISAIGYGHVGDGNLHLNVAVREYSKDVEKALEPFVYEHVSSKNGSISAEHGLGFQKKNYIGYSKNDQEIKMIKQLKNLYDPNAILNPYKYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBY1</accession>
    <name>C5DBY1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0A06248p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0A06248g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551730.1">
        <property type="nucleotide sequence ID" value="XM_002551684.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBY1"/>
    <dbReference type="FunCoup" id="C5DBY1">
        <property type="interactions" value="181"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBY1"/>
    <dbReference type="GeneID" id="8290535"/>
    <dbReference type="KEGG" id="lth:KLTH0A06248g"/>
    <dbReference type="eggNOG" id="KOG0504">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078327_0_0_1"/>
    <dbReference type="InParanoid" id="C5DBY1"/>
    <dbReference type="OMA" id="TPLHHCE"/>
    <dbReference type="OrthoDB" id="19174at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR21288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="202" mass="22520" checksum="CC54DA6DD4564517" modified="2009-07-28" version="1">MNIWIAASDGRAELVEKFLSEGFTAESKDPNGYTPIHAAASYGHIDLLRELCSAPYSGSVNVRDNDGDTPLHHCEDANTARVILEELGGDAKLTNNEGKTALAVCEEDGEFTDLIQYLRGVSGQQEEPLGIDSEQLAQFKDNLRYTLENDPETDDPQSIERRQKLEQILNGGNPEQDLENYIRDLVRSQLLADDEPSKRRKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBY8</accession>
    <name>C5DBY8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0A06424p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0A06424g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IMPACT family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551737.1">
        <property type="nucleotide sequence ID" value="XM_002551691.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBY8"/>
    <dbReference type="FunCoup" id="C5DBY8">
        <property type="interactions" value="423"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBY8"/>
    <dbReference type="GeneID" id="8290543"/>
    <dbReference type="KEGG" id="lth:KLTH0A06424g"/>
    <dbReference type="eggNOG" id="KOG3299">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045276_1_0_1"/>
    <dbReference type="InParanoid" id="C5DBY8"/>
    <dbReference type="OMA" id="WKPKFDW"/>
    <dbReference type="OrthoDB" id="69641at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140469">
        <property type="term" value="P:GCN2-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006446">
        <property type="term" value="P:regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23822">
        <property type="entry name" value="RWD_ScYIH1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.30">
        <property type="entry name" value="Impact, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023582">
        <property type="entry name" value="Impact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001498">
        <property type="entry name" value="Impact_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036956">
        <property type="entry name" value="Impact_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006575">
        <property type="entry name" value="RWD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16301">
        <property type="entry name" value="IMPACT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16301:SF25">
        <property type="entry name" value="PROTEIN IMPACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01205">
        <property type="entry name" value="Impact_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05773">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00591">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50908">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0346">Stress response</keyword>
    <keyword evidence="5" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="RWD" evidence="7">
        <location>
            <begin position="8"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR21295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="270" mass="29914" checksum="AB5C1245728A97AE" modified="2009-07-28" version="1">MSQEELQQELEAIDAIYPDFMERLSETQCVIKIPEHEAISVHLSFPTGYPTQSAPQVLQVNGAHDPKALRDQFEGVMEDIWKQDVCIFDFLTEVAEACSQVSDQDLLLSESDVQADAALQQQLESLQLEVSAAALDVTWFQSDPVVDRGSTFIAFAAHASSEEDACLKLDQLRMDPKVSKCQHVMTAWRIRGEGSVTYQDCEDDGETAAGGRLLHLLTLMDAWDVVVGVARWFTGTHIGPDRFKHINSTAREAVLRGSFADASKAGKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5DC02</accession>
    <name>C5DC02_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosome assembly protein 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Notchless protein homolog 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Ribosome biogenesis factor RSA4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0A06754g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the NLE1/RSA4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551751.1">
        <property type="nucleotide sequence ID" value="XM_002551705.1"/>
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        <property type="interactions" value="824"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DC02"/>
    <dbReference type="GeneID" id="8290558"/>
    <dbReference type="KEGG" id="lth:KLTH0A06754g"/>
    <dbReference type="eggNOG" id="KOG0271">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_57_16_1"/>
    <dbReference type="InParanoid" id="C5DC02"/>
    <dbReference type="OMA" id="AWEPYHR"/>
    <dbReference type="OrthoDB" id="10267436at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000092">
        <property type="entry name" value="notchless protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
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        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR036322">
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        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848:SF0">
        <property type="entry name" value="NOTCHLESS PROTEIN HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 9" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="NLE" evidence="11">
        <location>
            <begin position="32"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="138"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="181"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="224"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="273"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="397"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="439"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="481"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00061016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00068030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00077034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00080836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR21309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="514" mass="57172" checksum="BB8E9906EAB0B960" modified="2009-07-28" version="1">MATLVPPPSKKQKRQAQQPRQVDLIPEDLPHVLVKFQAFDTGESVGGSVRVPGAMTEQQLEQLLNQLHGETDEPVPYTFSCMVDAEKGKPEQLVDIKDNIYNSILKPGYKTTEDFITIVYTPRAVFKVRPVTRSSSAIAGHGSTILCSAFAPHTSSRMVTGAGDNTARIWDCNTNTPMQTLKGHFNWVLCVAWSPDGELIATGSMDNTIRLWQSEKGENFGDALRGHSKWITSLSWEPIHLVKPGDKPRLASASKDGTIKIWDTARRVCLFTLSGHTNSVSCVKWGGRGVLYSGSHDKTIRAWDMNAGGKCINILKSHAHWINHLSLSTDYALRVGAFDHTGKKPSSPEEARAKALENYEKIAKKNGKLEELMVTASDDFTMYLWDPIKSTKPITRMTGHQKLVNHVAFSPDGRYIVSASFDNSIKLWDGRDGTFIATFRGHVASVYQVAWSSDCRLLVSCSKDTTLKVWDVRTKKLSVDLPGHKDEVYTVDWSVDGKRVCSAGKDKMVRIWTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DC13</accession>
    <name>C5DC13_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Arp2/3 complex 34 kDa subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0A06996g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Functions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the Arp2/3 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cytoplasm</location>
            <location evidence="1 6">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ARPC2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551762.1">
        <property type="nucleotide sequence ID" value="XM_002551716.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DC13"/>
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        <property type="interactions" value="806"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DC13"/>
    <dbReference type="GeneID" id="8290569"/>
    <dbReference type="KEGG" id="lth:KLTH0A06996g"/>
    <dbReference type="eggNOG" id="KOG2826">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059439_1_0_1"/>
    <dbReference type="InParanoid" id="C5DC13"/>
    <dbReference type="OMA" id="YFDFQEE"/>
    <dbReference type="OrthoDB" id="148331at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005885">
        <property type="term" value="C:Arp2/3 protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030041">
        <property type="term" value="P:actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1460.20:FF:000009">
        <property type="entry name" value="Arp2/3 complex 34 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1460.20:FF:000010">
        <property type="entry name" value="Arp2/3 complex 34 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1460.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007188">
        <property type="entry name" value="ARPC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034666">
        <property type="entry name" value="ARPC2/4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12058:SF0">
        <property type="entry name" value="ACTIN-RELATED PROTEIN 2_3 COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12058">
        <property type="entry name" value="ARP2/3 COMPLEX 34 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04045">
        <property type="entry name" value="P34-Arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69645">
        <property type="entry name" value="Arp2/3 complex subunits"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR21320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="318" mass="36802" checksum="F142A7E9B1238A72" modified="2009-07-28" version="1">MLHLRPENLLVQKTLTEAWNASKAGSPLTLDRVISDFDYTTLHVSNSPDDKSILWLSIRTKAWQSVVKCGGDFAGYLKSKYNGFHAVSECSPEQGYDFTVQVKLDELDADAIVQLSLMKIHIMSYPFQLAFDEFARLSQLPEGSNASDTVHQIQYRDDENIFIKPSSDRITVIFETFFQDETDKVFGKVFLQEFVDARKRNRNIQSAPQVLFSHEPPLEAASLMAARSADKNRRFITFVLFPRHFQTPELQFSSVCHLSLFRNYFHYHIKCSKAYMHSRMRYRVDTFIKVLNRAKVDDEEEAGEQTTRRTITGRKLVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DC40</accession>
    <name>C5DC40_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Saccharopine dehydrogenase [NADP(+), L-glutamate-forming]</fullName>
            <ecNumber evidence="8">1.5.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Saccharopine reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0A07590g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-saccharopine + NADP(+) + H2O = (S)-2-amino-6-oxohexanoate + L-glutamate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10020"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57951"/>
            <dbReference type="ChEBI" id="CHEBI:58321"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.5.1.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (fungal route): step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the saccharopine dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.10" evidence="8"/>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551789.1">
        <property type="nucleotide sequence ID" value="XM_002551743.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DC40"/>
    <dbReference type="FunCoup" id="C5DC40">
        <property type="interactions" value="210"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DC40"/>
    <dbReference type="GeneID" id="8290596"/>
    <dbReference type="KEGG" id="lth:KLTH0A07590g"/>
    <dbReference type="eggNOG" id="KOG0172">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016207_3_1_1"/>
    <dbReference type="InParanoid" id="C5DC40"/>
    <dbReference type="OMA" id="WNYKFTW"/>
    <dbReference type="OrthoDB" id="10059875at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004755">
        <property type="term" value="F:saccharopine dehydrogenase (NADP+, L-glutamate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000008">
        <property type="entry name" value="Alpha-aminoadipic semialdehyde synthase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000072">
        <property type="entry name" value="Saccharopine dehydrogenase [NADP(+), L-glutamate-forming]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1870.10:FF:000002">
        <property type="entry name" value="Saccharopine dehydrogenase Lys9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1870.10">
        <property type="entry name" value="Domain 3, Saccharopine reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051168">
        <property type="entry name" value="AASS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032095">
        <property type="entry name" value="Sacchrp_dh-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005097">
        <property type="entry name" value="Sacchrp_dh_NADP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11133:SF22">
        <property type="entry name" value="ALPHA-AMINOADIPIC SEMIALDEHYDE SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11133">
        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16653">
        <property type="entry name" value="Sacchrp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03435">
        <property type="entry name" value="Sacchrp_dh_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Saccharopine dehydrogenase NADP binding" evidence="11">
        <location>
            <begin position="5"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Saccharopine dehydrogenase-like C-terminal" evidence="12">
        <location>
            <begin position="123"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00051869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00060549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00066976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00067598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00083134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF16653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR21347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="446" mass="49074" checksum="4147721D6DF3FF7F" modified="2009-07-28" version="1">MVKNVLLLGSGFVAQPVVDVLAGTEGINVTVGCRTLAKAQELAASSKSAAISVDVTKDDDLDAALAKHDLVISLIPYIYHAAVVKSAIRLKKDVVTTSYVSPALRELEPQIKEAGITVMNEIGLDPGLDHLYAVKTIDEVHRAGGKIKSFLSYCGGLPAPEDSDNPLGYKFSWSSRGVLLALRNSAKYWKDGKIEEVSSEDLMASAKPYFIFPGYALVCYPNRDSTPFKEFYKMPEADTVIRGTLRFQGFPEFVKVLVDVGMLKDDENEIFQKPISWKEALHQYWGSKSSSKDDLIAAIDSKTQWVTDEDRERILNGFSWLGLFSDNKINPKGNALDTLCATLEELMQFEEGERDMIILQHKFGIEWADGTPETRTSTLVAYGQPGGYSAMAATVGLPCAIATKLVLDGKIKGPGLVVPYSPEINDPIMKELKEKYNIFLKEKTIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DC57</accession>
    <name>C5DC57_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0B00154p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0B00154g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551806.1">
        <property type="nucleotide sequence ID" value="XM_002551760.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DC57"/>
    <dbReference type="GeneID" id="8290623"/>
    <dbReference type="KEGG" id="lth:KLTH0B00154g"/>
    <dbReference type="eggNOG" id="KOG1286">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007946_12_1_1"/>
    <dbReference type="InParanoid" id="C5DC57"/>
    <dbReference type="OMA" id="HWYGESE"/>
    <dbReference type="OrthoDB" id="10062876at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000001">
        <property type="entry name" value="Amino acid permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF15">
        <property type="entry name" value="GENERAL AMINO ACID PERMEASE AGP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="56"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="83"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="131"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="163"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="196"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="243"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="284"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="333"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="385"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="414"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="457"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="488"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="7">
        <location>
            <begin position="54"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR21367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="556" mass="61146" checksum="83D83D6033AA4C65" modified="2009-07-28" version="1">MSKKENFELAVTTLEEGETTTIHDVGAETIRSLEGQAPSLEERIARRKLFPRQVSMIGIGGAIGTALFVSIGTQIIHGGPGSLLIAFCLWSVVFIGLSQCMCVMVTYLPVTGSFVHFTERFVDSSCGFSVGWTYFVCQAANVCFEITAVCLVVEFWTDKIPKAALISILIALFGSLNLFSVFIFGEGEFYLSIGKVILAIGLIFFTIVVMAGGNPQHKVLGFKNWSNPGAFAEYISKGSAGKFHGFMSCLIFALYVFWGVDYLGNAASEAMNPRKVIPSSFRKVFGRLIIFYIGGAICVGILVPFNDHNMIKAISEGAVGAGASPYVSAMKTLGIGVLPHIVNVLILTSIISAGNSSLYSASRVLHRLALEGQAPKLFKITKRGVPIYCCVAVLLVCGLAYLSVSNSTNNVLTWFLNVETAAMAIVYIFICISYLQFAKGCKAQNIDLKTLPYSSSFLPYLGWHSLFWLVLMLFMNGYAVFLKDSWDLQSFIFSYFMIPFFIVLFAGHKLYKKTRFVKPVEMDLFSGIAELAEEDAYWEENPPKMNLLDKAFGFIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCC1</accession>
    <name>C5DCC1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0B01826p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0B01826g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551870.1">
        <property type="nucleotide sequence ID" value="XM_002551824.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCC1"/>
    <dbReference type="GeneID" id="8290698"/>
    <dbReference type="KEGG" id="lth:KLTH0B01826g"/>
    <dbReference type="HOGENOM" id="CLU_1200005_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCC1"/>
    <dbReference type="OMA" id="TSHYMTD"/>
    <dbReference type="OrthoDB" id="4036442at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR21432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="231" mass="26363" checksum="66603315A1070A98" modified="2009-07-28" version="1">MDSQAFSPSFEDELTRCATIIERFIVSLVNVAYYACFHKQNEDASPSTAAAKSAAFKKVRDSLLALAVRAEKLTSSEKISPADMKGLVCRDFLQELHRCSEVASDELLQVLNPITTKPLDGYEEPSSLNKIPTHLRNCILGFVQIFHFFRKLPVQEQYHISALQARILERELKSDLLGPWTRQVETLHSTIGWILLSDSHFQQKLNEYKSKTQTEPGALAFNEWLRHEIRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCF6</accession>
    <name>C5DCF6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Non-structural maintenance of chromosomes element 1 homolog</fullName>
            <ecNumber evidence="13">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0B02662g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Acts in a DNA repair pathway for removal of UV-induced DNA damage that is distinct from classical nucleotide excision repair and in repair of ionizing radiation damage. Functions in homologous recombination repair of DNA double strand breaks and in recovery of stalled replication forks.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="13">Component of the Smc5-Smc6 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the NSE1 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="13"/>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551905.1">
        <property type="nucleotide sequence ID" value="XM_002551859.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCF6"/>
    <dbReference type="FunCoup" id="C5DCF6">
        <property type="interactions" value="155"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCF6"/>
    <dbReference type="GeneID" id="8290736"/>
    <dbReference type="KEGG" id="lth:KLTH0B02662g"/>
    <dbReference type="eggNOG" id="KOG4718">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_826927_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCF6"/>
    <dbReference type="OMA" id="KIIRINH"/>
    <dbReference type="OrthoDB" id="185455at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030915">
        <property type="term" value="C:Smc5-Smc6 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011513">
        <property type="entry name" value="Nse1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014857">
        <property type="entry name" value="Nse1_RING_C4HC3-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20973">
        <property type="entry name" value="NON-SMC ELEMENT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20973:SF0">
        <property type="entry name" value="NON-STRUCTURAL MAINTENANCE OF CHROMOSOMES ELEMENT 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07574">
        <property type="entry name" value="SMC_Nse1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08746">
        <property type="entry name" value="zf-RING-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10 13" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="11 13" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 13" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9 13" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Non-structural maintenance of chromosomes element 1 RING C4HC3-type" evidence="14">
        <location>
            <begin position="239"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU368018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR21467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="289" mass="32575" checksum="9A5504B5021A8CAB" modified="2009-07-28" version="1">MEVDAVREESGGDEAQLSYEDKGKFLLQYVLSRRGVCSEKALAKALKTLERDGEQLEDSETEAQVVKMVGRLNVRLSALDYKLVRQRDQLGAWMYAYVDVSPSGDTKLATPLTIEELKYVQWCVREMLEAGQEVAEARGAKSAVERSVDEALVQSGSGRDELSAEFDSKSLRFRVSYTAGARQLFQFDDLGPLETERLLTRLCQLRWFYRTNEGHFGLATRALSELQEYLRTQYTVPVCTVCDEVALRGVKCQCAEGAWHAPCFQHYVAHVSNACARCERPVLEAIYMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCS0</accession>
    <name>C5DCS0_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">H/ACA ribonucleoprotein complex non-core subunit NAF1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0B05324g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NAF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552019.1">
        <property type="nucleotide sequence ID" value="XM_002551973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCS0"/>
    <dbReference type="FunCoup" id="C5DCS0">
        <property type="interactions" value="458"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCS0"/>
    <dbReference type="GeneID" id="8290857"/>
    <dbReference type="KEGG" id="lth:KLTH0B05324g"/>
    <dbReference type="eggNOG" id="KOG2236">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025072_1_0_1"/>
    <dbReference type="InParanoid" id="C5DCS0"/>
    <dbReference type="OMA" id="WKNDDEP"/>
    <dbReference type="OrthoDB" id="21550at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005732">
        <property type="term" value="C:sno(s)RNA-containing ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000493">
        <property type="term" value="P:box H/ACA snoRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001522">
        <property type="term" value="P:pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.230">
        <property type="entry name" value="Probable tRNA pseudouridine synthase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038664">
        <property type="entry name" value="Gar1/Naf1_Cbf5-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007504">
        <property type="entry name" value="H/ACA_rnp_Gar1/Naf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040309">
        <property type="entry name" value="Naf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31633">
        <property type="entry name" value="H/ACA RIBONUCLEOPROTEIN COMPLEX NON-CORE SUBUNIT NAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31633:SF1">
        <property type="entry name" value="H_ACA RIBONUCLEOPROTEIN COMPLEX NON-CORE SUBUNIT NAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04410">
        <property type="entry name" value="Gar1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="255"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="22"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="258"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="280"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="318"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="333"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="343"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="368"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="394"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="410"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="441"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR21581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="479" mass="53401" checksum="70CF5FFE49CBD04D" modified="2009-07-28" version="1">MCVDTFSKALLNPEEKPTGIEDNVDVGEISSDEEAENVTHSAEATAAVEESERTSSGESSMLNGAHEVSKSQVSEELPSLDAGEGSSSSEDEDSDDSSSSSSKEEGFLIDGLDEEDEEGDTVHGPIKSKNELNEEPIFEIPEDFELGPDTPIREIGFIKSAFDYNIIVQSVSSAEQRVLKENSLLCLEDRQILGPLCEVFGPLQSPFYRVLLPKSKNEQFEALRSKAGAKVFYVAPEAHWLDTFELKRMRGTDASNGFDEELPEDEQEFSDDEKEAEFKKLKKNSRKRKNENATNVGPIPSKKSVNSAHSKPKPRQVASAAFEYASSSSSYKPRNERQHETSRSTSAISQSQGSYPQQQHVPTPQPQYASQQTFGYPQQVYASHQMYSPPPQPAVQQFGNPFTHPYSQYPQMLPPQHLMPHPQHLPQHPQGQFPGSYPYTQAQSPPQQPVAAQTQNMDQVLQLQQILMNQQRQHQNQSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCY1</accession>
    <name>C5DCY1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0B06732p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0B06732g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552080.1">
        <property type="nucleotide sequence ID" value="XM_002552034.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCY1"/>
    <dbReference type="FunCoup" id="C5DCY1">
        <property type="interactions" value="829"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCY1"/>
    <dbReference type="GeneID" id="8290919"/>
    <dbReference type="KEGG" id="lth:KLTH0B06732g"/>
    <dbReference type="eggNOG" id="KOG1414">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027901_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCY1"/>
    <dbReference type="OMA" id="HNAQTES"/>
    <dbReference type="OrthoDB" id="295274at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14687">
        <property type="entry name" value="bZIP_ATF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051027">
        <property type="entry name" value="bZIP_transcription_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020956">
        <property type="entry name" value="TF_Aft1_OSM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19304">
        <property type="entry name" value="CYCLIC-AMP RESPONSE ELEMENT BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11785">
        <property type="entry name" value="Aft1_OSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="7">
        <location>
            <begin position="468"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="90"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="245"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="287"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="563"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="662"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="486"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="91"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="144"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="215"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="255"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="321"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="389"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="424"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="569"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="599"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="668"/>
            <end position="686"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR21642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="696" mass="74415" checksum="954EB82559312F6D" modified="2009-07-28" version="1">MNECQTGTALQRKCHAHSYWLRHTLALITRSSSPTGQLGTYCSAKHVPASTVLSSSARTDYRVAPERAQLSATRSSLPAARAQETVAATYRQAQPVTSPSAMRASDPPANDASLVSSFDLEPNPFEQSFASSDKPHGAGGAGVAGHQQVQRARQQSQSQSQSQPQSQPQSQPQLQGQSQQPTPSQPTLPSVHSAQSAASAQPKLPVIPTTPGGSRRLPPLLLANPAPAPATATTAVTATNALATPSAPQHGASAGGSQQAPQVPQASAGSATPGFFINLSKTGLTPNESGLRTGLTPGILTAAAPPHPPPPVSLPSGQFTPGLSSLLGLPLGQQPGQQQQAAQQPQQPQQTQQVSQQPAQQQQQQQQQQQQQQQQQQQQQQLPPHPAMSWSHSSSTAVSSDPSTTSSQNVKPRNNTSSSSSSSLAQDGTSALSLSEPAQKRQKLSSALPAKKPKNSEPSPLDVKDEQERKRKEFLERNRVAASKFRKRKKEYIKKIESDLQFYEAEYDDLSQCMDKLCGISKQTINSSLVGMLKQALLRHDVTSSLTVCNHIEQVLLQTKYVQRGGRNPRREEEEKRRMESPDADINSDSTYPRRASLATASTDGYHTSQLQPHQLQSHIRPDAGPQLSAADHATGTINNLPLVINGNTLLGLGDIQDPSHNAGAGTVTMSRPTSLTSLQPENNRSVPLHEYPNSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>C5DD09</accession>
    <name>C5DD09_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">D-aminoacyl-tRNA deacylase</fullName>
            <ecNumber evidence="2 6">3.1.1.96</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0B07370g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a D-aminoacyl-tRNA + H2O = a tRNA + a D-alpha-amino acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13953"/>
            <dbReference type="Rhea" id="RHEA-COMP:10123"/>
            <dbReference type="Rhea" id="RHEA-COMP:10124"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59871"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:79333"/>
            <dbReference type="EC" id="3.1.1.96"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>glycyl-tRNA(Ala) + H2O = tRNA(Ala) + glycine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53744"/>
            <dbReference type="Rhea" id="RHEA-COMP:9657"/>
            <dbReference type="Rhea" id="RHEA-COMP:13640"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78522"/>
            <dbReference type="EC" id="3.1.1.96"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the DTD family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.96" evidence="2 6"/>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552108.1">
        <property type="nucleotide sequence ID" value="XM_002552062.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DD09"/>
    <dbReference type="FunCoup" id="C5DD09">
        <property type="interactions" value="1650"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DD09"/>
    <dbReference type="GeneID" id="8290948"/>
    <dbReference type="KEGG" id="lth:KLTH0B07370g"/>
    <dbReference type="eggNOG" id="KOG3323">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076901_0_4_1"/>
    <dbReference type="InParanoid" id="C5DD09"/>
    <dbReference type="OMA" id="VFGADMK"/>
    <dbReference type="OrthoDB" id="275783at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051500">
        <property type="term" value="F:D-tyrosyl-tRNA(Tyr) deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106026">
        <property type="term" value="F:Gly-tRNA(Ala) deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00563">
        <property type="entry name" value="Dtyr_deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.80.10:FF:000001">
        <property type="entry name" value="D-aminoacyl-tRNA deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.80.10">
        <property type="entry name" value="D-tyrosyl-tRNA(Tyr) deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00518">
        <property type="entry name" value="Deacylase_Dtd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003732">
        <property type="entry name" value="Daa-tRNA_deacyls_DTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023509">
        <property type="entry name" value="DTD-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00256">
        <property type="entry name" value="D-aminoacyl-tRNA deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10472:SF5">
        <property type="entry name" value="D-AMINOACYL-TRNA DEACYLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10472">
        <property type="entry name" value="D-TYROSYL-TRNA TYR DEACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02580">
        <property type="entry name" value="Tyr_Deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69500">
        <property type="entry name" value="DTD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0820">tRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR21670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="150" mass="16470" checksum="945B151160D24F44" modified="2009-07-28" version="1">MRVVLQKVSKAAVTVDSEVVSAIKEGYMLLVGISVHDTIEDAEKISRKVLNLRIFEDENGAFWKKNVKEAGGQILSISQFTLQAVTKKGTKPDFHLAQKGPIAKGLYDEFLGLLRKDMGDENVKDGVFGAMMSCELVNEGPVTIVLDTKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDY6</accession>
    <name>C5DDY6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0C04840p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0C04840g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552435.1">
        <property type="nucleotide sequence ID" value="XM_002552389.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDY6"/>
    <dbReference type="FunCoup" id="C5DDY6">
        <property type="interactions" value="44"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDY6"/>
    <dbReference type="GeneID" id="8291302"/>
    <dbReference type="KEGG" id="lth:KLTH0C04840g"/>
    <dbReference type="eggNOG" id="ENOG502S129">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1133757_0_0_1"/>
    <dbReference type="InParanoid" id="C5DDY6"/>
    <dbReference type="OMA" id="HTCVAQI"/>
    <dbReference type="OrthoDB" id="4033908at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071986">
        <property type="term" value="C:Ragulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.30">
        <property type="entry name" value="Dynein light chain 2a, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020233">
        <property type="entry name" value="Slm4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16818">
        <property type="entry name" value="SLM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="245" mass="27122" checksum="F2E9876D95639CAB" modified="2009-07-28" version="1">MGSWSGELIALRSLPRLLQKATHGPARCTPGFCLALRACILFSNCSLFPSTSFVVSLAITCVMLYLIWETFNLLGSVVLLHEPHAPSTIIMLHGKNIHHLLELTLSPVQTVVAEHEPLFSSLLVSCTNGSIISYANSDTAPTTYNNSSNNLKMMALLVRDKWNEDQQDPSAQATHSCYQCEAEDGEVTRVYTYELEDLHACVAQIPRSDLLLLFIASSDYPYGLLVKKMKAALRAFSSMYGYKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>C5DE10</accession>
    <name>C5DE10_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Histidine--tRNA ligase, mitochondrial</fullName>
            <ecNumber evidence="3">6.1.1.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Histidyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">KLTH0C05390g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the aminoacylation of histidyl-tRNA in both the cytoplasm and the mitochondrion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>tRNA(His) + L-histidine + ATP = L-histidyl-tRNA(His) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17313"/>
            <dbReference type="Rhea" id="RHEA-COMP:9665"/>
            <dbReference type="Rhea" id="RHEA-COMP:9689"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78527"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.21"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.21" evidence="3"/>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552459.1">
        <property type="nucleotide sequence ID" value="XM_002552413.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DE10"/>
    <dbReference type="FunCoup" id="C5DE10">
        <property type="interactions" value="912"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DE10"/>
    <dbReference type="GeneID" id="8291326"/>
    <dbReference type="KEGG" id="lth:KLTH0C05390g"/>
    <dbReference type="eggNOG" id="KOG1936">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025113_4_2_1"/>
    <dbReference type="InParanoid" id="C5DE10"/>
    <dbReference type="OMA" id="CGGGNFK"/>
    <dbReference type="OrthoDB" id="1906957at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004821">
        <property type="term" value="F:histidine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006427">
        <property type="term" value="P:histidyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00773">
        <property type="entry name" value="HisRS-like_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.800:FF:000015">
        <property type="entry name" value="Histidyl-tRNA synthetase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000021">
        <property type="entry name" value="Probable histidine--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015807">
        <property type="entry name" value="His-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041715">
        <property type="entry name" value="HisRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004516">
        <property type="entry name" value="HisRS/HisZ"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00442">
        <property type="entry name" value="hisS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11476:SF7">
        <property type="entry name" value="HISTIDINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11476">
        <property type="entry name" value="HISTIDYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13393">
        <property type="entry name" value="tRNA-synt_His"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001549">
        <property type="entry name" value="His-tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="15">
        <location>
            <begin position="60"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="129"/>
            <end position="131"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="326"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="330"/>
            <end position="331"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00058343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00067413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR001549-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAR22021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="546" mass="60763" checksum="FFCAA2EF28DE5761" modified="2009-07-28" version="1">MGSMIDENIDIICWMSIAAINSTLLRQSGRAARRLSIMASTQQEKVGKSKVQFSLKTPKGTKDWSDKDVVIREEIFSRLSGLFKRHGGITLDTPVFELREILAGKYGEDSKLIYNLEDQGGELCSLRYDLTVPFARFVAMNNIQSMKRYHIAKVYRRDQPAMTKGRMREFYQCDFDIAGSYDSMIPDAEVLSIVVEGLSNLGIKDYKIKLNHRKILDGIFQVAGVSDENVRKISSAVDKLDKSPWEAVKHEMVVEKGQTEETADKIGEYVKLNGTLQEIYALLSQDSSITSNEKAKQGLDEMATLIKYSEALEIDSHISFDLSLARGLDYYTGIIFEAVTSASAPPKNAAELKKKAKGVEDASEYVGVGSIVGGGRYDNLVNMFAESTGKKSGQIPCVGLSFGVERIFSILKQRAEQASAIRPTATKVYVMAFGGGKDWAGYLPERMKVTKQLWNAGIATEFVYKAKSNPRKQFDAADKCGCPLAVILGKEEYLDNKLRVKRLGPEFANDDGELVDAADLVVVVKRKLDEIYQDDADEVTKLFRQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>C5DE16</accession>
    <name>C5DE16_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">KLTH0C05522p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0C05522g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552465.1">
        <property type="nucleotide sequence ID" value="XM_002552419.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DE16"/>
    <dbReference type="FunCoup" id="C5DE16">
        <property type="interactions" value="186"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DE16"/>
    <dbReference type="GeneID" id="8291332"/>
    <dbReference type="KEGG" id="lth:KLTH0C05522g"/>
    <dbReference type="eggNOG" id="KOG0955">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002663_1_0_1"/>
    <dbReference type="InParanoid" id="C5DE16"/>
    <dbReference type="OMA" id="CKYWSMK"/>
    <dbReference type="OrthoDB" id="20839at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15492">
        <property type="entry name" value="PHD_BRPF_JADE_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000007">
        <property type="entry name" value="Bromodomain containing 1, isoform CRA_b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019542">
        <property type="entry name" value="Enhancer_polycomb-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034732">
        <property type="entry name" value="EPHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050701">
        <property type="entry name" value="Histone_Mod_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13793:SF107">
        <property type="entry name" value="BROMODOMAIN-CONTAINING PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13793">
        <property type="entry name" value="PHD FINGER PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10513">
        <property type="entry name" value="EPL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13831">
        <property type="entry name" value="PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13832">
        <property type="entry name" value="zf-HC5HC2H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51805">
        <property type="entry name" value="EPHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="10">
        <location>
            <begin position="239"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="11">
        <location>
            <begin position="293"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="441"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="535"/>
            <end position="568"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR22027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="721" mass="82201" checksum="9DE4E8777920D3C5" modified="2009-07-28" version="1">MSSSQATQLKDEGPKLREEKHFADFYPELDQQEPLPIIVTNEIKDDANLRNVETKEARHIKQMIFKERVTLESINIGANRAKFKKCRFKVSSQGINNHPRISKNYQKFGYISGSLADKFNEHDTPYIKKTDSLALIKDNPLGHISRYQSNFRVEYDMDEQDELYLRFLNESKSITPNNKLTHEIFEIAMTALENEWFFLEKKIPPRISTTSEVSTRETRAAWAHYEAFGSDDGTGHTIDQPCAVCGGTECDNSNAIVFCDGCDVAVHQECYGVVFIPEGQWLCRRCMISRNRKINCLFCPSHTGAFKQTDTGSWGHVVCGLWIPELYFVNSHYMEPIEGVDLIPRSRWKLTCYICRKKVGACIQCSNKNCFCAYHVTCAKRSALCMDFGSCSIIEASSNAIPPGLKLLSFCDKHSPPGWPSCEEGIQKTRLYFASADNDANSGSGSQASYPHSSTKAKWTTNRGTPIAPSTFASVVQRILEMFQVENSAKVSADLCKYWSMKRELKRGAPLVRKFDPTSFSTFTVEQLGKRIEFADRLSKDLEMLQDMAQLLVERQMTTRRRNKAEEKIQNLYHHPSRFILQESVIKKFQNSAPYKALLREKPIGARMSAILRDCTSSDSHDEITQFKKGMTEFLDRIETEQNVPRRVKIEAKKLRAHIAVLLASVEGFDYRSWISEDFTIEGGRVKAVPWKGPALMERENLDAVEELSQAEMRKLRSLLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5DE27</accession>
    <name>C5DE27_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Kynurenine 3-monooxygenase</fullName>
            <ecNumber evidence="11">1.14.13.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Biosynthesis of nicotinic acid protein 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Kynurenine 3-hydroxylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">BNA4</name>
        <name evidence="14" type="ordered locus">KLTH0C05786g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the hydroxylation of L-kynurenine (L-Kyn) to form 3-hydroxy-L-kynurenine (L-3OHKyn). Required for synthesis of quinolinic acid.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>L-kynurenine + NADPH + O2 + H(+) = 3-hydroxy-L-kynurenine + NADP(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:20545"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57959"/>
            <dbReference type="ChEBI" id="CHEBI:58125"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.14.13.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="11">Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from L-kynurenine: step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Mitochondrion outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the aromatic-ring hydroxylase family. KMO subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.14.13.9" evidence="11"/>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552476.1">
        <property type="nucleotide sequence ID" value="XM_002552430.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DE27"/>
    <dbReference type="FunCoup" id="C5DE27">
        <property type="interactions" value="828"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DE27"/>
    <dbReference type="GeneID" id="8291344"/>
    <dbReference type="KEGG" id="lth:KLTH0C05786g"/>
    <dbReference type="eggNOG" id="KOG2614">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023210_0_1_1"/>
    <dbReference type="InParanoid" id="C5DE27"/>
    <dbReference type="OMA" id="REFMFIA"/>
    <dbReference type="OrthoDB" id="10053569at2759"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00328"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004502">
        <property type="term" value="F:kynurenine 3-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034354">
        <property type="term" value="P:'de novo' NAD+ biosynthetic process from L-tryptophan"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043420">
        <property type="term" value="P:anthranilate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070189">
        <property type="term" value="P:kynurenine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006569">
        <property type="term" value="P:L-tryptophan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019805">
        <property type="term" value="P:quinolinate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000129">
        <property type="entry name" value="Kynurenine 3-monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01971">
        <property type="entry name" value="Kynurenine_monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027545">
        <property type="entry name" value="Kynurenine_monooxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46028">
        <property type="entry name" value="KYNURENINE 3-MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46028:SF2">
        <property type="entry name" value="KYNURENINE 3-MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0274">FAD</keyword>
    <keyword evidence="2 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 11" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="8 11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6 11" id="KW-0521">NADP</keyword>
    <keyword evidence="7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3 11" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="432"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding" evidence="13">
        <location>
            <begin position="4"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR22038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="463" mass="51984" checksum="33F8A26DD32FD09D" modified="2009-07-28" version="1">MPETVAVIGAGLVGCLAAVALAKRGYEVTLFDYRLDPRLATTQDRNLRSINLAISARGITSLEYVDKGISKRVLKDIIPMQGRMIHNLRGHQEAQNYGLFGECINSIDRGVLNNSLLDEVDNLQNVALKFGHKLSQIKFTPNAKQTCVFTTNAASAAKEYAFDFVLGCDGAYSSTRSQMQRELRMDFSQEYIDCCYIELYVPPAKEHNPKFNGKFAISPNHLHIWPRGNYMLIALANGDGSFTSTFFGPWSLVEGLDTRSKIQDFLTNNFPDAVEIIGLKQAVDAFENHPRGALMCVECSPYHLSQGRAIILGDAAHSMVPFYGQGMNCGFEDVRVLMQLLDKHSGNRAAAFEEYSSKRHTDLVSIIKLAKNNYKEMSHGVISNLYLLRKKLDTLLGRVLKDRWLPLYTMVSFRADIPYHKAIQIEQRQKKILAYLQAFILALTSLGGLGLYKVLRRALKNRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEI6</accession>
    <name>C5DEI6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0C09526p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0C09526g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552635.1">
        <property type="nucleotide sequence ID" value="XM_002552589.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEI6"/>
    <dbReference type="GeneID" id="8291513"/>
    <dbReference type="KEGG" id="lth:KLTH0C09526g"/>
    <dbReference type="HOGENOM" id="CLU_1402676_0_0_1"/>
    <dbReference type="InParanoid" id="C5DEI6"/>
    <dbReference type="OrthoDB" id="10327850at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR22197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="194" mass="21756" checksum="B0A8E996098111EA" modified="2009-07-28" version="1">MSALVNSNFEEEVGEYVSRAKFRKRIRSWLSTSKEDWAGASGCEKHTAKEVSYDTEQLDFLLRLSGLQETQEDPRRDSLSLTSAEKLCSSVLSSGDEAEPTTRRGRILQFYRKLKSSFHRPSAGSLPGLREKHASKPWINLQEGEANFPELPQSKTDGAGLSDFVSPPCLSHDQMCTDVAVTRLSLEQWSLRDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEQ8</accession>
    <name>C5DEQ8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0C11308p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0C11308g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NPR2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552707.1">
        <property type="nucleotide sequence ID" value="XM_002552661.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEQ8"/>
    <dbReference type="FunCoup" id="C5DEQ8">
        <property type="interactions" value="228"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEQ8"/>
    <dbReference type="GeneID" id="8291593"/>
    <dbReference type="KEGG" id="lth:KLTH0C11308g"/>
    <dbReference type="eggNOG" id="KOG3789">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014995_3_0_1"/>
    <dbReference type="InParanoid" id="C5DEQ8"/>
    <dbReference type="OMA" id="IDVNWDP"/>
    <dbReference type="OrthoDB" id="338854at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990130">
        <property type="term" value="C:GATOR1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904262">
        <property type="term" value="P:negative regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010508">
        <property type="term" value="P:positive regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009348">
        <property type="entry name" value="NPR2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12991:SF10">
        <property type="entry name" value="GATOR COMPLEX PROTEIN NPRL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12991">
        <property type="entry name" value="NITROGEN PERMEASE REGULATOR 2/TUMOR SUPPRESSOR CANDIDATE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06218">
        <property type="entry name" value="NPR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="346"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="391"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="576"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="399"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR22269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="690" mass="77596" checksum="834CB324310CCC6F" modified="2009-07-28" version="1">MSGHFDGFVPVHGIFYSVFHPTEGTKVRHQFPSGCLELSDITFDTIKNYVIPKPQLCNKLLTFKYGPFRLVCYPVNVVAPYYARNSFSFDFVFVFPYDCATSPYEPAIERLGKMFTVLEEQSQILSKAENSSVYFQLKARNTQKHGNTETAKGTEQPSSLDIGEIAQPENQGYASAKYYEILKDLEGPSKKIAIGDLITKIYRDLNDYSECLIPIDSGNAVDIKLFPLLSPPSSCFSIEDVPISTVNLAKLIDVNWDPTMLKIVPHINGINSIAKVAKFSDSDVNLVVECIKHLIYYNCVILTDIFQFSNIYASTNELSRFLTEPTLAAECQIYVAYNESAHLPNLPFERGPSRSGQGNIRDRSSNGTLDILRKGKQTPLRENSFNSGMDFLSNKHQHTPQSRSSSIPYHNTSDASFGNSDAGLKGKLMPFTSKSCLFDLYRSLTQGQTIREWYKTHFEKIRAARIDVRRFITFGVLHGLIYRSYSYPVLRNIGFAESVEALGATNHSRYAKYKHLLKTNQPLNNVLSAADIRIGLENAGFKARSEKLKQHSTADAAEEVLSDVYRKLALSDKKTDHVAQGNNPISKVFGRNSEDSTDISTRESANSMGSDIKNKVAFDVRKTLATSSADERRRSEILRSIEEKRNEELILLKSVRSVHNFDKICTLLGKDRERVESMLSELGPYNVLHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DET9</accession>
    <name>C5DET9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 9">Imidazoleglycerol-phosphate dehydratase</fullName>
            <ecNumber evidence="4 9">4.2.1.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0D09702g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + H2O</text>
            <dbReference type="Rhea" id="RHEA:11040"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57766"/>
            <dbReference type="ChEBI" id="CHEBI:58278"/>
            <dbReference type="EC" id="4.2.1.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 6/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the imidazoleglycerol-phosphate dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.19" evidence="4 9"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553132.1">
        <property type="nucleotide sequence ID" value="XM_002553086.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DET9"/>
    <dbReference type="FunCoup" id="C5DET9">
        <property type="interactions" value="268"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DET9"/>
    <dbReference type="GeneID" id="8295370"/>
    <dbReference type="KEGG" id="lth:KLTH0D09702g"/>
    <dbReference type="eggNOG" id="KOG3143">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044308_3_0_1"/>
    <dbReference type="InParanoid" id="C5DET9"/>
    <dbReference type="OMA" id="GIPFFDH"/>
    <dbReference type="OrthoDB" id="447729at2759"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00011"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004424">
        <property type="term" value="F:imidazoleglycerol-phosphate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd07914">
        <property type="entry name" value="IGPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.40:FF:000005">
        <property type="entry name" value="Imidazoleglycerol-phosphate dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.40:FF:000001">
        <property type="entry name" value="Imidazoleglycerol-phosphate dehydratase HisB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.40">
        <property type="entry name" value="Imidazole glycerol phosphate dehydratase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00076">
        <property type="entry name" value="HisB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038494">
        <property type="entry name" value="IGPD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000807">
        <property type="entry name" value="ImidazoleglycerolP_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020565">
        <property type="entry name" value="ImidazoleglycerP_deHydtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002114">
        <property type="entry name" value="PRK00951.2-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23133:SF2">
        <property type="entry name" value="IMIDAZOLEGLYCEROL-PHOSPHATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23133">
        <property type="entry name" value="IMIDAZOLEGLYCEROL-PHOSPHATE DEHYDRATASE HIS7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00475">
        <property type="entry name" value="IGPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00954">
        <property type="entry name" value="IGP_DEHYDRATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00955">
        <property type="entry name" value="IGP_DEHYDRATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 9" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="8 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR22694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="227" mass="24587" checksum="A2EBF16EFABC8C32" modified="2009-07-28" version="1">MNGTSNQGKTERRSFVSRNTNETKIQIALSINGGHVEIKDSILPKKAADIASQATSSQIIDIHTGVGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGEAFKEAVGAVRGVKRFGHGYAPLDEALSRAVVDLSNRPYAVVDLGLKREKIGDLSTEMIPHFLESFAEAARITLHVDCIRGFNDHHRSESAFKALAIALRESLSSNGTNDVPSTKGVLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DF34</accession>
    <name>C5DF34_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Phosphatidylinositol N-acetylglucosaminyltransferase GPI3 subunit</fullName>
            <ecNumber evidence="4">2.4.1.198</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">GlcNAc-PI synthesis protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0D11880g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalytic subunit in the complex catalyzing the transfer of N-acetylglucosamine from UDP-N-acetylglucosamine to phosphatidylinositol, the first step of GPI biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.4.1.198" evidence="4"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553227.1">
        <property type="nucleotide sequence ID" value="XM_002553181.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DF34"/>
    <dbReference type="FunCoup" id="C5DF34">
        <property type="interactions" value="490"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DF34"/>
    <dbReference type="GeneID" id="8295467"/>
    <dbReference type="KEGG" id="lth:KLTH0D11880g"/>
    <dbReference type="eggNOG" id="KOG1111">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_19_0_1"/>
    <dbReference type="InParanoid" id="C5DF34"/>
    <dbReference type="OMA" id="SHFWMSG"/>
    <dbReference type="OrthoDB" id="734129at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000506">
        <property type="term" value="C:glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017176">
        <property type="term" value="F:phosphatidylinositol N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03796">
        <property type="entry name" value="GT4_PIG-A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000026">
        <property type="entry name" value="Phosphatidylinositol N-acetylglucosaminyltransferase subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039507">
        <property type="entry name" value="PIG-A/GPI3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013234">
        <property type="entry name" value="PIGA_GPI_anchor_biosynthesis"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45871">
        <property type="entry name" value="N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL BIOSYNTHETIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45871:SF1">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08288">
        <property type="entry name" value="PIGA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PIGA GPI anchor biosynthesis" evidence="12">
        <location>
            <begin position="36"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="11">
        <location>
            <begin position="187"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00068617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF08288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR22789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="437" mass="49087" checksum="DACDA0C72DCF8DBB" modified="2009-07-28" version="1">MVCDFFYPQLGGVEFHIYHLSQKLISLGHLVVIITHAYGDRTGVRYLTNGLKVYHIPYFVAYRETAFPTIFGSFLIVRNILIREQIQIVHTHVSVSPLTHETLLHANVMGLQTLLTDHSLYGFDTLSEILNNKMLKFALTNTGAVICVSNTCKENMIIRSKLNPSSISVIPNAVVSHDFTPATKGSKAPGEITIVVISRLYPNKGADLLTHIIPRICEIEDKVKFIIGGDGPKFVDFQQMIESYRLQDKVQLVGAVQHENVRDVLCRGDIYLHASLTEAFGTALVEAASCGLLIVTTTAGGIPEVLPKHMTVFAQETSVSCLVAATRKAIQSLREGTVDTTSFHEEISSMYDWVDVAKRTVAVYDSLAQSPMPRNKSWYKMLKNFHERNDGPWAMPLYVLCYLVENMLYLFLEWWYPRSEIDLAPKWPINQNHAASP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFB4</accession>
    <name>C5DFB4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0D13728p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0D13728g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553307.1">
        <property type="nucleotide sequence ID" value="XM_002553261.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFB4"/>
    <dbReference type="FunCoup" id="C5DFB4">
        <property type="interactions" value="23"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFB4"/>
    <dbReference type="GeneID" id="8295549"/>
    <dbReference type="KEGG" id="lth:KLTH0D13728g"/>
    <dbReference type="eggNOG" id="ENOG502S12B">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103058_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFB4"/>
    <dbReference type="OrthoDB" id="4055611at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097196">
        <property type="term" value="C:Shu complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000725">
        <property type="term" value="P:recombinational repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031779">
        <property type="entry name" value="Psy3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16836">
        <property type="entry name" value="PSY3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR22869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="182" mass="20852" checksum="0C946533C49EE781" modified="2009-07-28" version="1">MTKIKSSVVHRLIDELPNQTFVLMIDIASCWREFATEYSHEEKSVAYMNSASLLHFESLINFLAQLNDNPQEALSRCKQAAPLQFQLASIVIDNISYYTHDNASYELLFKILRMLRSRYGCSVMTVGFGLDFYNGVEQSFATNKSYDIPTRLPMSYVKNMDCVLQRDNASTARVVYTKNSMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFN5</accession>
    <name>C5DFN5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0D16566p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0D16566g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytochrome b5 family. MAPR subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553428.1">
        <property type="nucleotide sequence ID" value="XM_002553382.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFN5"/>
    <dbReference type="GeneID" id="8295676"/>
    <dbReference type="KEGG" id="lth:KLTH0D16566g"/>
    <dbReference type="eggNOG" id="KOG1110">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070889_2_1_1"/>
    <dbReference type="InParanoid" id="C5DFN5"/>
    <dbReference type="OMA" id="YGKWGAY"/>
    <dbReference type="OrthoDB" id="10257697at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050577">
        <property type="entry name" value="MAPR/NEUFC/NENF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10281:SF76">
        <property type="entry name" value="CALCUTTA CUP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10281">
        <property type="entry name" value="MEMBRANE-ASSOCIATED PROGESTERONE RECEPTOR COMPONENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="3">
        <location>
            <begin position="93"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR22990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="197" mass="21566" checksum="11A9CDC1A2E5B460" modified="2009-07-28" version="1">MLRRRSTRHRSPAAGPGRGRSTSDGATARSRFTWLDIARMLCGLALAVLAVGRLATGSATWHVVATWRARASAPSAPPALSEFWRPYSLPLTFSPADLSRYTGADGAPLLVAVDGQVFDVTRSARLYGPRGAYHRFVGRDCSRAFAYSIWSMRGLREPCSADLSGLATEERARVVAWAEYFARKYPRVGHVAHVTQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFR9</accession>
    <name>C5DFR9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0D17402p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0D17402g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR23024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553462.1">
        <property type="nucleotide sequence ID" value="XM_002553416.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFR9"/>
    <dbReference type="FunCoup" id="C5DFR9">
        <property type="interactions" value="51"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFR9"/>
    <dbReference type="GeneID" id="8295712"/>
    <dbReference type="KEGG" id="lth:KLTH0D17402g"/>
    <dbReference type="eggNOG" id="KOG4784">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029122_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFR9"/>
    <dbReference type="OMA" id="QAMKVFY"/>
    <dbReference type="OrthoDB" id="386949at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030332">
        <property type="term" value="F:cyclin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031624">
        <property type="term" value="F:ubiquitin conjugating enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051865">
        <property type="term" value="P:protein autoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006513">
        <property type="term" value="P:protein monoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019193">
        <property type="entry name" value="UBQ-conj_enz_E2-bd_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31531:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE E3D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31531">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE E3D FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09814">
        <property type="entry name" value="HECT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR23024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="352" mass="40168" checksum="9672976DF78BC85C" modified="2009-07-28" version="1">MKFLAEYLPRIRTVVITIESPKEVHVKGLEPESVELANDQGARYVIELPHRVVIDSSQNSFQSYKDYKTLRLRAVEDKGDRDLYKNRSLNFMMQVVPQRWMKRDLLGEEFQLRCKDCESCVLAKKDCVQINEMPSEFWAELMDYWHCHKPASSDGMRVDRYAKLSPLPGELLVGNSFFLISSSWPGNQLSFEGDNTACSKCSSVLGCLSTDNLYKINKWNLGLVRNGRKEVYPPEHFVISALLDLLNADGARKIKLKCQDSSQHLLLWIFAVGLQVTLPLGNRVSNCMKVYYKEEKVDESAINASKVQNIEELAVPAIAFSSFIEKLKTAHQQLPAVVKKMDNWSLGFVEYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFS6</accession>
    <name>C5DFS6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0D17556p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0D17556g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR23031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553469.1">
        <property type="nucleotide sequence ID" value="XM_002553423.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFS6"/>
    <dbReference type="STRING" id="559295.C5DFS6"/>
    <dbReference type="GeneID" id="8295719"/>
    <dbReference type="KEGG" id="lth:KLTH0D17556g"/>
    <dbReference type="eggNOG" id="KOG3009">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012201_3_0_1"/>
    <dbReference type="InParanoid" id="C5DFS6"/>
    <dbReference type="OMA" id="FNCIIAT"/>
    <dbReference type="OrthoDB" id="198885at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016798">
        <property type="term" value="F:hydrolase activity, acting on glycosyl bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004730">
        <property type="term" value="F:pseudouridylate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1790.10">
        <property type="entry name" value="Indigoidine synthase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01876">
        <property type="entry name" value="PsiMP_glycosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022830">
        <property type="entry name" value="Indigdn_synthA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007342">
        <property type="entry name" value="PsuG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42909:SF1">
        <property type="entry name" value="CARBOHYDRATE KINASE PFKB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42909">
        <property type="entry name" value="ZGC:136858"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04227">
        <property type="entry name" value="Indigoidine_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110581">
        <property type="entry name" value="Indigoidine synthase A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0464">Manganese</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carbohydrate kinase PfkB" evidence="6">
        <location>
            <begin position="351"/>
            <end position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR23031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="730" mass="78367" checksum="9C511D777385A7DD" modified="2009-07-28" version="1">MLKSAKALGLRGFARKLCISDEVRSALSLGKPVVALESTIITHGLPFPQNMQMAKNAESIIRNNHAVPATIAFMNGVPKVGLSLHEIEQLAGSAGSLPINKVSRRDIAYTMANNLFGGTTISSTMILAHMAGIQIFATGGLGGVHRGAEVTMDISADLEELGKTPVAVVCAGPKAILDIEKTMEFLETKGCMVATLGPPGVKVPGFYCRESNVTSPYSFETFKDAARIIESGHQLGLGSGSLLCIPPPPEVALEDSFIEAVIDKASHKAEKMGIKGKQLTPFLLSEIAHKTNGLSVSSNIDFVMNNAKAGSQIACELSKLQSPDKNLTYFQPSGVYDGIQADERVNSEKLNIAVVGAIALDTHCSSLNEIRMADSNPGKIGSAIGGVGYNVALAAHLSNRYQSVSTSLVSSLGNDLSGSAICEKMKLNSKTIYRDDKNPTAQYLSVHSSGGELVVACSDMDIIAKLPPSYVSDRLESLAPKVVIADANVSVEVLNLLMVLQEKLGYKLIFEPTSDTKARKLSQLSNLSPFPDHRFYTITPTVSELESIFEAFKEANKFDVNKWFPVLDSLQIDAALKKLRSGENPAVMQQLTKKGILQMACYLLPYFPNILIKDGANGLFLVTVKDCMSDIKGKASLANFSFLAEGLQVNGKKRGVLFEHYEVPEKSVVKNVTGAGDTLLGVLSNEICFKGDIFDAEPLQRYECTRRAQLGAKLTVEDCGAVSENLARLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DG23</accession>
    <name>C5DG23_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0D01826p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0D01826g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552803.1">
        <property type="nucleotide sequence ID" value="XM_002552757.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DG23">
        <property type="interactions" value="521"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DG23"/>
    <dbReference type="GeneID" id="8295023"/>
    <dbReference type="KEGG" id="lth:KLTH0D01826g"/>
    <dbReference type="eggNOG" id="KOG1879">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002668_1_0_1"/>
    <dbReference type="InParanoid" id="C5DG23"/>
    <dbReference type="OMA" id="FIWRSTC"/>
    <dbReference type="OrthoDB" id="27683at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003980">
        <property type="term" value="F:UDP-glucose:glycoprotein glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018279">
        <property type="term" value="P:protein N-linked glycosylation via asparagine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040497">
        <property type="entry name" value="Glyco_transf_24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009448">
        <property type="entry name" value="UDP-g_GGtrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040694">
        <property type="entry name" value="UGGT_TRXL_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040692">
        <property type="entry name" value="UGGT_TRXL_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11226">
        <property type="entry name" value="UDP-GLUCOSE GLYCOPROTEIN:GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11226:SF0">
        <property type="entry name" value="UDP-GLUCOSE:GLYCOPROTEIN GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18404">
        <property type="entry name" value="Glyco_transf_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18401">
        <property type="entry name" value="Thioredoxin_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18402">
        <property type="entry name" value="Thioredoxin_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950459" evidence="6">
        <location>
            <begin position="18"/>
            <end position="1324"/>
        </location>
    </feature>
    <feature type="domain" description="UGGT thioredoxin-like" evidence="7">
        <location>
            <begin position="240"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="UGGT thioredoxin-like" evidence="8">
        <location>
            <begin position="403"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="domain" description="Glucosyltransferase 24 catalytic" evidence="9">
        <location>
            <begin position="1064"/>
            <end position="1282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF18401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF18404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR22365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1324" mass="151673" checksum="442FCD37E5F577A8" modified="2009-07-28" version="1">MLLWTLLVWSYAIFASCTTVRLSNASSGVKLRLWNAVVGLVNDEQLVQDLYFLIAGVSDELDELKTDSTPLDQIVEALARHNPELASLLSLRYALEPGFSNDTSNQNYFLLNNKRFENADDIFYLKTDDLNAQKSACINDVLDNEDVVMGSNRDAPVILFYGCDRVEDWEEFNRNLYMEAQAGKVRFVWRSTCPNDLPFEFEPSAVALTVKGSQWNTEFGHALDVPESLVLGTEPSFRAQQVSDEQLQDLDMKVGTLVYDFYEKSQNATETIRYFRQIVNNFPVLARSLAEQGDITIDNRWEAALKKLAKEGIDNTMLGLFVNGQFHKLSNLNKWSVLQSVILELQRVNFLMDLLQKYQNITPGESLVLAKKLLNTFSKRSLTTQQLLQPVKYDLHRIPGFSESIIYFNDIELDSDYAEKLKDDIAMFFEESEFGELPAYRENWNEIVFVIDFSDLRSKDTWEALQGLLRALQVVKKGYPQRIGLLPFSTSAQDHGVLGKIYELKQNKPLETLVSYLEDLSPIERPQQSVFQKLPPVLEIINDRLKINETSIVINGEIYPFKSNLWNYLVSKVLKKDVSFIKSELLRINTKNFKAREILHAKSFIERNKKYAPDYFEDATYSNTNVTALLSLAHRIVETSTGGGLNTIHTVTIVEDFNTPLGLQRLIHLLQIKLHGVRVRAVHSGLLGKNWDKLKFTISNADTSKLQNYLRSLKSNGECNYIEPAIVGQWLLDLPRKILQRTSYLAVNGRFIHFEQGELPTIYEYEMIVQREATRVLDAVASLQEVFPDSLKEPLDPNYVEMVSAVLAKMFYDGERLYNNGIDFTAEASISRINLGELLPSESFGAFQTSEEPRKVEVTLILDPLEERTQNLLALASYLGSLDFLKLQVVLMPTTDLKVFPNHRLWIEGEKDLSIIDTRKFYVDVEVPPYLHVGERKDNVFELEYIKVEVNAFENKEEPSKGLVEGIGNVCLNLLNANHEVIDTATTTDTFGYTQFRLRRLEQGLTIESCSSNYRVSSFTSNSKADYIASDHFDVTSLAPTRLYVRLEKTQGMLPEVSEDNRIINILSIIENAEDEVSFEKQASSVMRSRGSFDTKFWIILGEEPSASFLSFVEFMTAESAGEVTFEYLTVDWPRWLRPQRFRPRQLAAAKVMMLDTLFPKGVTKVVYMSPGARVPNITELWEYDFDSVFCAPRAYQRDGTPYWKQGYWRNFLSKNNLKFHATEPLFIVNLGKYRQEHAGSKFRIHYQRLSPGINFLVQLDQDLLNDMQTLFPISSLNKRLLKVAPHLQTLDDNLYQSLQEKFNAKKSGQKLEDLSETIVHDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DG28</accession>
    <name>C5DG28_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Magnesium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0D01936g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">High-conductance magnesium-selective channel that mediates the influx of magnesium into the mitochondrial matrix. Essential for the splicing of mRNA group II introns in mitochondria by affecting mitochondrial magnesium concentrations, which are critical for group II intron splicing. It also suppresses a variety of mitochondrial intron mutations and its absence may disturb the assembly of mitochondrial membrane complexes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Homopentamer. Forms homooligomers. Interacts with MFM1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Mitochondrion inner membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the CorA metal ion transporter (MIT) (TC 1.A.35) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552808.1">
        <property type="nucleotide sequence ID" value="XM_002552762.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DG28"/>
    <dbReference type="FunCoup" id="C5DG28">
        <property type="interactions" value="383"/>
    </dbReference>
    <dbReference type="GeneID" id="8295028"/>
    <dbReference type="KEGG" id="lth:KLTH0D01936g"/>
    <dbReference type="eggNOG" id="KOG2662">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025144_1_0_1"/>
    <dbReference type="InParanoid" id="C5DG28"/>
    <dbReference type="OMA" id="TLLIHMF"/>
    <dbReference type="OrthoDB" id="10251508at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045016">
        <property type="term" value="P:mitochondrial magnesium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12823">
        <property type="entry name" value="Mrs2_Mfm1p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.340:FF:000005">
        <property type="entry name" value="Inner membrane magnesium transporter MRS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.340">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045863">
        <property type="entry name" value="CorA_TM1_TM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039204">
        <property type="entry name" value="MRS2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13890:SF27">
        <property type="entry name" value="MAGNESIUM TRANSPORTER MRS2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13890">
        <property type="entry name" value="RNA SPLICING PROTEIN MRS2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22099">
        <property type="entry name" value="MRS2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144083">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 14" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 14" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="300"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="330"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00046105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00046701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU366042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR22370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="427" mass="49072" checksum="2238EA737B9DCFFB" modified="2009-07-28" version="1">MIWSRLRPFRLLAKPFLRSRRLYHRLLPEQNSGILSLKPITPNDAFVSCTLFNKNGAVTAVSQKFPKWAFLKDHNLYPRDLRKIDTTSVDVIPSIVVKPTCILINLLHIKALIQHDCVFVFDTSNSEAAMKLGVLMYDLESKLSTNPNAHMAQLYEHRALESILMNVMTSLETEYKQHYSICGIILKDLEDEISRDKLRDLLIKSKNLTSYYKKSLLIRDVLDELLDSDEDLAAMYLGEHKNENDDFADLEMLLETYYKQCDEYVQQSETLLQDIRSTEEIVNIILDANRNALMLFELKVTIYTLGFTIATLVPAFYGMNLKNFIEDSPLGFGCVVGFSVIAALCVTWSNFKALSSVKRLTMMNNHTGVNTARHAANARHELNQEAPILWKKWASSLRTVWLGSNHPVRNGKHRDLIWKWLVDDNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGB4</accession>
    <name>C5DGB4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">pH-response regulator protein palF/RIM8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0D03938g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the arrestin family. PalF/RIM8 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552894.1">
        <property type="nucleotide sequence ID" value="XM_002552848.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGB4"/>
    <dbReference type="FunCoup" id="C5DGB4">
        <property type="interactions" value="44"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGB4"/>
    <dbReference type="GeneID" id="8295119"/>
    <dbReference type="KEGG" id="lth:KLTH0D03938g"/>
    <dbReference type="eggNOG" id="ENOG502QTQN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006001_1_0_1"/>
    <dbReference type="InParanoid" id="C5DGB4"/>
    <dbReference type="OMA" id="GMSIEIV"/>
    <dbReference type="OrthoDB" id="7785529at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070086">
        <property type="term" value="P:ubiquitin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011021">
        <property type="entry name" value="Arrestin-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050357">
        <property type="entry name" value="Arrestin_domain-protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188">
        <property type="entry name" value="ARRESTIN DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188:SF161">
        <property type="entry name" value="PH-RESPONSE REGULATOR PROTEIN PALF_RIM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00339">
        <property type="entry name" value="Arrestin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01017">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="4">
        <location>
            <begin position="250"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="220"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="460"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="225"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="472"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00037950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00040066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR22456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="550" mass="61096" checksum="A13E5EE5C02C2C3F" modified="2009-07-28" version="1">MGFLTKLKAVSSRNSDPSISSTKVSAVSDAKTRYNYAKRSRFRHACAARDFYINQESPHKVWKPNEQISGEAVLVLKDDLENVAVRLSLIGEVRIRGSGSKTHCDQLFEKTTLVYGDQEEVAPETRSIVNGLTKGEHRFPFRLKVPSKNIFTSIKFERGSISYCIRCTLEPLRCSDPEKVLAACEKSISVMVPLDVSDMPKPNTKSVVLQSPSAVKQIKLANGEQDASSSFTRRTGGSKNSGSTVNTSGSLKTVTINVDLPSSGYVIGEVIPVKLNLCHYREYSHSTGLIATLVRICRVGGASKENPMEMYRKDICQTVSPIYVNPDTFECSVTVYLKIPLDAFPTLRLPDRKFSFQYYVEVLVNLSRKNIAFTESNRVVDTTTNSHVSASPGKTIEETFTLLQRKIRSGSETEPFKNNDRESMIFFKDLINVDKLKRLRNVTGMSIEVIVGTNRSEIPQDSSLDEVEENPETTNSGLQSIDYTNTAVNNYTVAGYSPLGDCKDVLNFDGGSVLPLPVYTPNSHYNAADDKDELELQRLQELESEPPMAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGD7</accession>
    <name>C5DGD7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein RACK1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Guanine nucleotide-binding protein subunit beta-like protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0D04466g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat G protein beta family. Ribosomal protein RACK1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552917.1">
        <property type="nucleotide sequence ID" value="XM_002552871.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGD7"/>
    <dbReference type="FunCoup" id="C5DGD7">
        <property type="interactions" value="1503"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGD7"/>
    <dbReference type="GeneID" id="8295143"/>
    <dbReference type="KEGG" id="lth:KLTH0D04466g"/>
    <dbReference type="eggNOG" id="KOG0279">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_57_7_1"/>
    <dbReference type="InParanoid" id="C5DGD7"/>
    <dbReference type="OMA" id="NCKLKIN"/>
    <dbReference type="OrthoDB" id="7875889at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005092">
        <property type="term" value="F:GDP-dissociation inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045182">
        <property type="term" value="F:translation regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000039">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit beta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045223">
        <property type="entry name" value="RACK1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19868">
        <property type="entry name" value="RECEPTOR FOR ACTIVATED PROTEIN KINASE C RACK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 6" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="13"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="61"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="103"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="144"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="192"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="291"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR22479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="319" mass="35117" checksum="401B17E851C650FD" modified="2009-07-28" version="1">MSSAEVLVLRGTLEGHNGWVTSLATSPAQPNLLLSGSRDKSLITWKLTGDDQQYGVPVRSFRGHSHIVQDCTVTPDGEYALSASWDKTVRLWELASGKCIQRFVGHKSDVLSVTIDRRASQIVSASRDKTVKVWNTLGECMVTLLGHNDWVSQVRVAPNETPEDETVTVISAGMDKVVKVWNLHSFQIEADFIGHNNYVNTVTASPDGTLVASAGKDGQIMLWNLTEKEALYTLNAQDEVFAVAFSPNRYWLTAATSSGIKIFNLEERKVVDELKPEFAGYTKAAEPHAISLAWSADGQTLFAGYTDNVIRVWQVMTAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGK0</accession>
    <name>C5DGK0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0D05962p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0D05962g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552980.1">
        <property type="nucleotide sequence ID" value="XM_002552934.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGK0"/>
    <dbReference type="FunCoup" id="C5DGK0">
        <property type="interactions" value="20"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGK0"/>
    <dbReference type="GeneID" id="8295210"/>
    <dbReference type="KEGG" id="lth:KLTH0D05962g"/>
    <dbReference type="eggNOG" id="KOG2401">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_590497_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGK0"/>
    <dbReference type="OMA" id="NDDHESK"/>
    <dbReference type="OrthoDB" id="4080456at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14279">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003892">
        <property type="entry name" value="CUE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052772">
        <property type="entry name" value="Endo/PolyKinase_Domain-Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002625">
        <property type="entry name" value="Smr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036063">
        <property type="entry name" value="Smr_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46535">
        <property type="entry name" value="NEDD4-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46535:SF1">
        <property type="entry name" value="NEDD4-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00463">
        <property type="entry name" value="SMR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160443">
        <property type="entry name" value="SMR domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51140">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50828">
        <property type="entry name" value="SMR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CUE" evidence="3">
        <location>
            <begin position="2"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="Smr" evidence="2">
        <location>
            <begin position="364"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR22542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="458" mass="50934" checksum="6996DB426ED7F03E" modified="2009-07-28" version="1">MMSSQDLALLAELFPSVNENKLRGTLKSAGGDAQVACSMILSEQETLNQMAKNSLSNNTDGRSEGEGMGSRHAKALSEENFPDENHTKARKGAVNLKKRMWRPIHSFTKGQSSRLELDQAAAVHSNAWVSATDSIQDIIHYTNAAPKSAQKAFYKKTLNSARAIIDIIYHYDEYAEDINGSRLPGDNEPEYGTPQRDFMKAGGRVQSPTGFAHKKKPVGLVFSADQERANIFSPRSEENYKYDSSSLEAKELNSIITNNPSLRAISPSFSKRALEFYEGDVERTTLTLIFIIEKNCAQYTFVDAASKAPSLNSQKNLSKVNKGSVKPRRMGAEIGGLDPSCFASDENYDRALLIIDNILTMYTADLHGFLPYEAELIAERCLNVWWTKELALREMNGQRLNQIKAINLAPFKIITGRGLHSVGGVSKVRIKVKKFLDTCLYSYTEEASYFIVEGKKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-04-02" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGP1</accession>
    <name>C5DGP1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0D06886p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0D06886g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553021.1">
        <property type="nucleotide sequence ID" value="XM_002552975.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGP1"/>
    <dbReference type="STRING" id="559295.C5DGP1"/>
    <dbReference type="GeneID" id="8295252"/>
    <dbReference type="KEGG" id="lth:KLTH0D06886g"/>
    <dbReference type="eggNOG" id="KOG1940">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013368_7_1_1"/>
    <dbReference type="InParanoid" id="C5DGP1"/>
    <dbReference type="OMA" id="CIERATM"/>
    <dbReference type="OrthoDB" id="411372at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16464">
        <property type="entry name" value="RING-H2_Pirh2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039512">
        <property type="entry name" value="RCHY1_zinc-ribbon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008913">
        <property type="entry name" value="Znf_CHY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037274">
        <property type="entry name" value="Znf_CHY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017921">
        <property type="entry name" value="Znf_CTCHY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037275">
        <property type="entry name" value="Znf_CTCHY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21319:SF0">
        <property type="entry name" value="AND RING FINGER DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G08900)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21319">
        <property type="entry name" value="RING FINGER AND CHY ZINC FINGER DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05495">
        <property type="entry name" value="zf-CHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14599">
        <property type="entry name" value="zinc_ribbon_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161219">
        <property type="entry name" value="CHY zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161245">
        <property type="entry name" value="Zinc hairpin stack"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51266">
        <property type="entry name" value="ZF_CHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51270">
        <property type="entry name" value="ZF_CTCHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CHY-type" evidence="7">
        <location>
            <begin position="242"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="CTCHY-type" evidence="8">
        <location>
            <begin position="318"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="385"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="106"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="214"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="592"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="106"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="119"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="592"/>
            <end position="612"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR22583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="664" mass="73572" checksum="7B1E34345DDFD498" modified="2009-07-28" version="1">MSDSASESGRSLAEEESEDELIMSAFDASFEKIGRGFPRISFPSIWGAKSAGPGTKDEVEGDFGRWSRRFQMNTFLNDLQKGLSFSNALTRLQTVKAITEEAISNAINSSDTGHGDNSASREDGDADKPEVPAGVVPAAKPKNVPAYNDMVIPQGKGPQVLDEHLLKLTELPSDQNNDEATLRQRIQHIRSLDLGPAKTAAMMQRLMMGKFVDELNRPSPTPTLEAPLTRDPSEAEKAPTYHNTGALGCAHYQRNCKLQCHQCLGWYTCRFCHDESPEISGSSHPHSFQRAETRQVMCMRCNHVQAPQKDCENCDEEFAMYFCPECKLYDNDETKDIYHCAKCGICRLGLGLGLDFFHCDGCQACLSIELQGNHKCIERATMSSCPICGDFMFTSVKPVVYMSPCGHAIHQHCFDEYTKHSYKCPSCQVSILNMEAQFRVLDKEIEEQPLPAPYCDWICYVSCNDCKAKSTCKYHILGLRCGNCMSYNTSQLKLVKPEEAATHDNAAEFDTITQSNSSFDSLNVMRQELLRGQFQREEVSPLITVEKGGMLSNIEQYMNGYFHDDTQGASEGASADHGDFSSQNLFNTAGFFKNNNRNENQSGDATVGSSSMARAPSLTERLRQFINDAPPQLSISDAFQKFIQGSHQGLSSGDEDDCSSQASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGV4</accession>
    <name>C5DGV4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">KLTH0D08580p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0D08580g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553084.1">
        <property type="nucleotide sequence ID" value="XM_002553038.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGV4"/>
    <dbReference type="FunCoup" id="C5DGV4">
        <property type="interactions" value="95"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGV4"/>
    <dbReference type="GeneID" id="8295320"/>
    <dbReference type="KEGG" id="lth:KLTH0D08580g"/>
    <dbReference type="eggNOG" id="KOG2398">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_434790_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGV4"/>
    <dbReference type="OMA" id="RFAKSWN"/>
    <dbReference type="OrthoDB" id="27823at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120104">
        <property type="term" value="C:mitotic actomyosin contractile ring, proximal layer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00174">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065:SF7">
        <property type="entry name" value="NOSTRIN, ISOFORM H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065">
        <property type="entry name" value="PROLINE-SERINE-THREONINE PHOSPHATASE INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00611">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00055">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="F-BAR" evidence="11">
        <location>
            <begin position="1"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="10">
        <location>
            <begin position="533"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="284"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="320"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="398"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="139"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="284"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="326"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="354"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="414"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="428"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR22646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="600" mass="68823" checksum="A4991C1D3C97C3E8" modified="2009-07-28" version="1">MSHSYNYQLSFWDEQDTGTRILLEHVSQGLESCEGLIDFFEERSKLEKDYARRLSAITAKFNSNIQSQPDYGKLGLSVKSLHSSQERLSQSHSKQAVAIYRDGFSELKLFVQDLQARYKTISLKTRNLCNDKITKKQLCDVLQGKLEKASTELRDCQLNQDNYLGKRDSSQNDRHIQKWRSIVDELQMKLDVLKQEYKNSTKHWLHEWSRLSLELQELERSRIQFIKVKLQEFSKQCSDAAVEEQISMDNLTQQLTKFTADQDISSFAYNHGTGRIRGNFVHAASADSPRTSSTSSKHVQNVRQLSSQLQRSRLSGYYEEQAADVPHPEVEKQKPEPTKLDRLEPPETAAEVYTSSESSNESSNPTEFTSNVRHRASVESMSTSISSLAHSIGDSQRFAKSWNSQNRRKSKTRSQLLESSQSSNLNEGSRTSSMETTRFHASSGPRTTQRRKSMVASDSSSNPFKEALEAMKRGSSGYDDVEDERPSNSTSSQKKHLIPFQHSRSSTNETCTKRVMDKDHYVELPLFSSKGEEVLRYAKALYTFMEPNEQQIVNFHVGDYLLLTEQLDQDWFIGEVLDSQNVDPEYRYGIIPRNYIEILP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGV9</accession>
    <name>C5DGV9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 10">M-phase inducer phosphatase</fullName>
            <ecNumber evidence="2 10">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0D08690g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the MPI phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2 10"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553089.1">
        <property type="nucleotide sequence ID" value="XM_002553043.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGV9"/>
    <dbReference type="FunCoup" id="C5DGV9">
        <property type="interactions" value="296"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGV9"/>
    <dbReference type="GeneID" id="8295325"/>
    <dbReference type="KEGG" id="lth:KLTH0D08690g"/>
    <dbReference type="eggNOG" id="KOG3772">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_496118_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGV9"/>
    <dbReference type="OMA" id="DKCETEM"/>
    <dbReference type="OrthoDB" id="26523at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000086">
        <property type="term" value="P:G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010971">
        <property type="term" value="P:positive regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110032">
        <property type="term" value="P:positive regulation of G2/MI transition of meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01530">
        <property type="entry name" value="Cdc25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000021">
        <property type="entry name" value="M-phase inducer phosphatase cdc-25.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000751">
        <property type="entry name" value="MPI_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10828">
        <property type="entry name" value="M-PHASE INDUCER PHOSPHATASE DUAL SPECIFICITY PHOSPHATASE CDC25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10828:SF17">
        <property type="entry name" value="PROTEIN-TYROSINE-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00716">
        <property type="entry name" value="MPIPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 10" id="KW-0132">Cell division</keyword>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="6 10" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="12">
        <location>
            <begin position="271"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="34"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="83"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="166"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="90"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00067190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU368028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR22651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="572" mass="64262" checksum="60C431BFE37052B6" modified="2009-07-28" version="1">MSDPKIFVRKAKRTTLQEGGNFLKNINLLVGINRRKSGKSRAARGGDAPGLNHPQSNLQQNETEKTTFAADSVFGAQVLHISSENSSTSCEEDDEEKGEGEGESCNSPVVRQPFFPILDSPSEDVFEHSMRLKPQRAPAPFLARIGNERANSASLAAIKNSDGFNLRSRPLKRQPSGVKKEHATSQVAKGQLRRIHSMYASQKEVSKGLEVPKMRSLEEPEAECDIECPAYESKLERSGIPHYFDNKTDDNLPRISVETLVNIMDGDVKKQYDTVHIVDCRFEYEYQGGHIGDAINISSQTSLESVFIHNRKNYCKSHLPSLVIFHCEFSSYRGPIMASHLRNCDRMLNYDNYPKLHYPDILIVEGGYKSFFEKYSHRCFPRRYIEMGSQDHIDKCESEMEKFRRNSKRVATKSNSLHSLNKGNVTSRASTPFRSEFASRNQSCFSDYKKHDRPGLSHYTTKSNLSFRYEAPPKLTLSQFSMNSGSGSPSSVSSSAASSRALLLDELQDDLESSDFDNISFEENEHPHTYCGGTDPVLALNSPKASLLSSIFQEEEVDSRYPSKQLANATTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-04-02" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>C5DH44</accession>
    <name>C5DH44_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0E01232p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0E01232g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553542.1">
        <property type="nucleotide sequence ID" value="XM_002553496.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DH44"/>
    <dbReference type="STRING" id="559295.C5DH44"/>
    <dbReference type="GeneID" id="8291680"/>
    <dbReference type="KEGG" id="lth:KLTH0E01232g"/>
    <dbReference type="eggNOG" id="KOG2992">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040344_1_1_1"/>
    <dbReference type="InParanoid" id="C5DH44"/>
    <dbReference type="OMA" id="ENNSGME"/>
    <dbReference type="OrthoDB" id="5599646at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007718">
        <property type="entry name" value="Srp40_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05022">
        <property type="entry name" value="SRP40_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Srp40 C-terminal" evidence="2">
        <location>
            <begin position="355"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="27"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="120"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="291"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="314"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR23105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="427" mass="42155" checksum="5CE003B7F117F337" modified="2009-07-28" version="1">MASKKSGKVAKSEAPKLSEKMKKEELSTSGSSSSSSSSSSSDSSSSRSSSSDESSSSSSSSSSSSSSSSSSSSSSSSSSDSELSTSSGESSNEEKSKPGNKRKKSPKTAKAAKKQKLSKKSSSSESSSSSSSSGSSSSSSDSESSSSSSASSDESSSSSSSDSESSSSGSSSSSSGSSSSSSGSSSSSSDSDSSSSDSDSSSSGSDSSSSSSDSSSSDSDSSSSDSDSSSSGSDSSSSGSDSSSSGSSSSSSDSDSSSSSSGSSSSDSDSSESDASSSSSSDDEAEKSQLKSHSSPAGSSSSTKKNSKHDGETQETSQGSSSSSKTASPDAPSPAAPKDGIPAAVDELKPGQRKHFSRIDKNAIKFEDRVLTDNTYKGAAGTWGEKANEKLGRVRGKDFTKNKNKMKRGSYRGGSITLSSGSYKFTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DH84</accession>
    <name>C5DH84_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0E02134p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0E02134g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553582.1">
        <property type="nucleotide sequence ID" value="XM_002553536.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DH84"/>
    <dbReference type="FunCoup" id="C5DH84">
        <property type="interactions" value="312"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DH84"/>
    <dbReference type="GeneID" id="8291721"/>
    <dbReference type="KEGG" id="lth:KLTH0E02134g"/>
    <dbReference type="eggNOG" id="KOG1036">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038526_2_0_1"/>
    <dbReference type="InParanoid" id="C5DH84"/>
    <dbReference type="OMA" id="TPEKVYT"/>
    <dbReference type="OrthoDB" id="10262475at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002372">
        <property type="entry name" value="PQQ_rpt_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10971">
        <property type="entry name" value="MRNA EXPORT FACTOR AND BUB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13360">
        <property type="entry name" value="PQQ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Pyrrolo-quinoline quinone repeat" evidence="4">
        <location>
            <begin position="63"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="259"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR23145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="341" mass="37946" checksum="CE8722C4E6B28970" modified="2009-07-28" version="1">MLNHICTKTLKFKTSLARKVRHQRPNCKLGMQFECLKNVPEDLVSSLLVSDRTPHINVTAWDGTVSLYDYNANELVVRMKHTDPLLCSAWLEHGSKKYAGSVAGEVLEVDMESGKFHLVSDAAELGISAMRCSENDVLAGSWDGSLQALDTRSGKAWFTVRHENRKVLALDCAGNTVVVATTGGKVTIYDLRNMHNPKLQESGLKFQTRDIKLMPSGGGYVQSSLDGRVAVEYFGQDSSRFAFRCHRMNLSDTQFVFPVNALCFNRSDELLYTGGSDGRVFSWNLTTRKKSEELPKFEDSVLKLGCNDDVFCVATGDDSFKTLATIQDADVQPGKIYYTKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>C5DH93</accession>
    <name>C5DH93_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="17">KLTH0E02398p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">KLTH0E02398g</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the XPG/RAD2 endonuclease family. EXO1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553591.1">
        <property type="nucleotide sequence ID" value="XM_002553545.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DH93"/>
    <dbReference type="FunCoup" id="C5DH93">
        <property type="interactions" value="208"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DH93"/>
    <dbReference type="GeneID" id="8291733"/>
    <dbReference type="KEGG" id="lth:KLTH0E02398g"/>
    <dbReference type="eggNOG" id="KOG2518">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008978_5_0_1"/>
    <dbReference type="InParanoid" id="C5DH93"/>
    <dbReference type="OMA" id="WLHRAAC"/>
    <dbReference type="OrthoDB" id="26491at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035312">
        <property type="term" value="F:5'-3' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017108">
        <property type="term" value="F:5'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09908">
        <property type="entry name" value="H3TH_EXO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09857">
        <property type="entry name" value="PIN_EXO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000011">
        <property type="entry name" value="exonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000002">
        <property type="entry name" value="Exonuclease 1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036279">
        <property type="entry name" value="5-3_exonuclease_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037315">
        <property type="entry name" value="EXO1_H3TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008918">
        <property type="entry name" value="HhH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044752">
        <property type="entry name" value="PIN-like_EXO1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006086">
        <property type="entry name" value="XPG-I_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006084">
        <property type="entry name" value="XPG/Rad2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006085">
        <property type="entry name" value="XPG_DNA_repair_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081:SF65">
        <property type="entry name" value="DNA DAMAGE-INDUCIBLE PROTEIN DIN7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081">
        <property type="entry name" value="FLAP ENDONUCLEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00867">
        <property type="entry name" value="XPG_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00752">
        <property type="entry name" value="XPG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00853">
        <property type="entry name" value="XPGRADSUPER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00279">
        <property type="entry name" value="HhH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00484">
        <property type="entry name" value="XPGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00485">
        <property type="entry name" value="XPGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0267">Excision nuclease</keyword>
    <keyword evidence="8" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XPG N-terminal" evidence="16">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="XPG-I" evidence="15">
        <location>
            <begin position="139"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="449"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="518"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="628"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="454"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="518"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="641"/>
            <end position="650"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAR23154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="665" mass="74190" checksum="6A46C2D3AA611945" modified="2009-07-28" version="1">MGIQGLLPQLKPIQQPITLIRYDGQTLAIDGYAWLHRAAHSCAEELALGRPTSKYLEFFIKRLNMLRNRFNINPYLVFDGDAIMVKKDTELKRKQKRAENKERALALWKAGDKRQAYDYFQKCVDVTPEMAKCVIEYCQVQDIKYIVAPFEADAQMVYLEKKGLVHGIISEDSDLLIFGCRKLITKLTDHGEGIEICRDDFPRLPSKFPLSQLCPEETRAMVCLSGCDYTAGIPKIGLLTAMKLVRKHKTMDNIIKNIQREGKFVVPKEFLEEYQLASFAFQFQRVYCPENGMMTTLSEIPEELRNCQALFSCIGKVISKSEHTKVVIVDDSEVDHHLHGRIAAGELCPYNFQKILVNRERKLQLTTKSEPIIGKPLASQAGSIDTFFSKTASVSVSSTSTGVTTQMRFAIKKQEEKLIENERKIASAVERRKLFRRDTPVVAGVGMSRYFGKSQDHPTNEEKDVTSTMATPTPVEPLISPRLPDFTKASTNSNFASCSNESLGSQDEAATDIPSSLLSTEVPSSMVPTQVDMESSPYSEEDSEVLSEIEDTAEARRDEKASNKRHCNSIADLRESFSYHRAPLQDKSVNVASGNGLLRNSKGAKSHVSAVKAPSLGLNSHVGAVKAPSLGTKRPRHYVASPSSATAPRQATRTLSLSDFIYRGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHB7</accession>
    <name>C5DHB7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0E02992p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0E02992g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553615.1">
        <property type="nucleotide sequence ID" value="XM_002553569.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHB7"/>
    <dbReference type="STRING" id="559295.C5DHB7"/>
    <dbReference type="GeneID" id="8291760"/>
    <dbReference type="KEGG" id="lth:KLTH0E02992g"/>
    <dbReference type="HOGENOM" id="CLU_296305_0_0_1"/>
    <dbReference type="InParanoid" id="C5DHB7"/>
    <dbReference type="OMA" id="FLHENTE"/>
    <dbReference type="OrthoDB" id="1879at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR23178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1033" mass="117184" checksum="07C3D16ED71BD1AF" modified="2009-07-28" version="1">MLNESLMDVFGILNDRSGPTDEARVYKQLWELLKRDPTSIHALLSHEFMNRPIEELAGCVAQECLEKRLVCLCVLDFRLRSGCVLPSPSDALSSIFSTVSMVQADPQATNACFRILSTVLAFAESTQTVGLRQCDVDCISSEHLVSENAAVLMDYVYLALTHPWISESEAQQWLSELYRSGVFWWLRQKLQSTAKLDFLETLFEDTMKRAYNSFIQTMQTFLRAQGIAKEWQFCGASKFSELFDPMVLKNCVEDALAALKIQPRTRDAGTAPRLLAHLSKRPIQSRDFWQGIMLDRPALDLMWISPSRDMFTHASKTYMHEFAKLQCDIKNFILRINSRYSISHGKLTGNSKYATSAYELGQDPVTKSWFIKVAKTFAPEAQVEKWGNIQGKRLCLSRLDGAFFVVQVASVHKVGDMLQLFIGTENVSGLQNCQLLSVLSTELEFQVKAVDDLLKFNLENERLQAMTPMTNLVRSSCPSKTVILSNLFSDEQQIKNFLHENTESVKQKPSKRRLVPVKEAISLDLEKGKWAIEKAVKNSPKHDFTLEQIESVIHGVCESASCVNGDVGTGKATIIAAIIDNLHLNSFYKRFPQMCSRFLIVCDTDETCQKIASYLSIIQPQSVVHYSNDTSSLIEKKKKGVKEALSRVQEIASILRIPGDHSSSVPAALSFLQHFLEPMLHDYRASIKTNDEISKRAFPLLEDGVLTSPSSEMAERIIETSYQVAVMYDQLQKWQFLFLNGDLEKTLTEACDLLLISKDRFQKCLVDKKLSFNSFESLVICNADFFEPSEIWRALSLNTHWQRTIFSGDFTNSPLPYVARQLFSSKSLQFRLTTSYNTNREVLRIRFPYEEQPDPVPVLYSPVQVIKTPGKIVNNINWDEAEYCVLLYAYLRLAGYPAKVISIIALNPYQKYAIDTELEDWLQLEPSHKDIKKPSSISSLENNRSHRNDIVIISTAGIQPNVPFLSRYARRGLLVLSAAGDDLQIVAGESFPSVKSDKREARRIQNLDQLKGLVDSLLCSKRPRKQSRKTHNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHJ8</accession>
    <name>C5DHJ8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0E04884p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0E04884g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="CAR23259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_002553650.1"/>
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        <property type="interactions" value="663"/>
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    <dbReference type="KEGG" id="lth:KLTH0E04884g"/>
    <dbReference type="eggNOG" id="KOG0296">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="HOGENOM" id="CLU_000288_57_9_1"/>
    <dbReference type="InParanoid" id="C5DHJ8"/>
    <dbReference type="OMA" id="SIWDYSK"/>
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        <property type="component" value="Chromosome E"/>
    </dbReference>
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        <property type="entry name" value="Ribosome assembly protein SQT1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
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        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051179">
        <property type="entry name" value="WD_repeat_multifunction"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19857:SF8">
        <property type="entry name" value="ANGIO-ASSOCIATED MIGRATORY CELL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19857">
        <property type="entry name" value="MITOCHONDRIAL DIVISION PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
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        <property type="entry name" value="WD40 repeat-like"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="78"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="16"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR23259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="439" mass="47789" checksum="0F80791CD0B9128E" modified="2009-07-28" version="1">MSEYPSEQEHQTGDQIPEEEFITNQEVDQEVVLDDNDNDGDFPVDEDDLEEIDGAGEDAEDEEDTIEIDMSNNSIAYFDKHTDSVFSIFAHPTLPLVCSGGGDNTAYLWTSHSQPPKFAGSIAHSESVIGGGFTPDGQFLITADMNGQILVHKASNGGAKWKKCAELQEVQEVVWIKVHPTVSGVFAFGSTDGSVWCYQVDSQSGELEQIMSQFVHQSDCTMGEFINVEQGDANLSLVTCSLDSTIIGWSCYTNQQIFKVTSSEIRGYEAPWVSLAKAPVSMTKNTPVVACGANNGTMAILNCSNGAVLQLSIVIELQADQDELDASIESIAWSEKLPLMAVGLVSGEVLLYDALTWRIRRKFVLPDSVTKLQFEDSAATTLLASCINGKVYQYDARTGSEKFVCVGHNMGVLDFVLIENGKKLITAGDEGVSLVYQLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHQ5</accession>
    <name>C5DHQ5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0E06226p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0E06226g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553753.1">
        <property type="nucleotide sequence ID" value="XM_002553707.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHQ5"/>
    <dbReference type="FunCoup" id="C5DHQ5">
        <property type="interactions" value="134"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHQ5"/>
    <dbReference type="GeneID" id="8291906"/>
    <dbReference type="KEGG" id="lth:KLTH0E06226g"/>
    <dbReference type="eggNOG" id="ENOG502QUGB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016668_0_0_1"/>
    <dbReference type="InParanoid" id="C5DHQ5"/>
    <dbReference type="OMA" id="LNKTMHV"/>
    <dbReference type="OrthoDB" id="410044at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12453">
        <property type="entry name" value="RRM1_RIM4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034352">
        <property type="entry name" value="Rim4_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21245">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="150"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="370"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="38"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="452"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="562"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="80"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="456"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="482"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="571"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="606"/>
            <end position="636"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR23316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="775" mass="83399" checksum="601927DCB7B3FF34" modified="2009-07-28" version="1">MSLESATTAIDPTAAFAAQTSAKDGLLVPDVSDSIAQFLPTEVSSSESEDEEEAGSAAGGSARPPTASDPHPESCGAPESESEFLEELDDISDGEGAGAASKEKDSAPGAGAGAGAGAGAASGIASAHVAPRSGIAASEAAPKFRGRPSSCVFVASLAASLGDDDLCLSVTENFKKFGRLTMVKVLRDPANRPYAFVQYTNDADAKKALKEAQGSSLNGRTIRCGPARVNRTLFVCYKGRNLNSSGLVSAAFVNQLLEKFGELEQLVACRDQFFHRKNNAYSGSDRSNAWFIQFAYRDDAIRAFANLKSDLNWVVEWAQNIEAPSHMNMLSDSKNLVIREETEPENEGACIMPADSYSESSGSEVVIDQKSIFVGQLDPMATEEGVLERFSRHGSILELNLISKPNNVFAFIRFTTEEAAAAALEMENHAMFLNKTMHVQYREIGGIKKFRRGNYNHRNNNNGVHGNHNPSGSGNGSRHESTSNSQAYNQATYQPPRLNLAPPPINVGKRRSSTGSGNYNHHYQHYPFGHEFQVYAHQPHQLSMMGGPKKSKASLRHRNVEITKSIDESNNDSTNTSSNNNNNDTDNLNGGASEKSKGLPNELKSAASQSFVTDVTSDTSGSAGNNDASTNASTSRYTYSAGGINYKKKLDEYKKRGNYPTFDPYYYPPYYYPVEFPLNPMGPSGLAGRAGPGAPLSASGSPAHPGNQPYFMYYPLPQGSGMEFADMHNMMVPHLGGSPGPMGSPIFPLPISQQHFIPSDRSVSFNGEEKAALEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>C5DI10</accession>
    <name>C5DI10_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0E08778p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0E08778g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553858.1">
        <property type="nucleotide sequence ID" value="XM_002553812.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DI10"/>
    <dbReference type="GeneID" id="8292019"/>
    <dbReference type="KEGG" id="lth:KLTH0E08778g"/>
    <dbReference type="eggNOG" id="ENOG502QV53">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_329013_0_0_1"/>
    <dbReference type="InParanoid" id="C5DI10"/>
    <dbReference type="OMA" id="VYWEMPR"/>
    <dbReference type="OrthoDB" id="5121955at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052073">
        <property type="entry name" value="Amide_Lactam_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47171">
        <property type="entry name" value="FARA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47171:SF3">
        <property type="entry name" value="FARA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="8">
        <location>
            <begin position="18"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="80"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="634"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="688"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="777"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="853"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="93"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="645"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="716"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="794"/>
            <end position="805"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR23421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="873" mass="97295" checksum="FDA5B7B0F739830D" modified="2009-07-28" version="1">MNSEKSAYRVRKPRTSRACEVCHDRKVRCDANIRVPCTSCQTFGLVCRLRDVKKVKRGKKVGGAAGGAEAGLKAEDDAEVGAHPSAGADGCAPGHGHGHGPGQGAGQGRENEEEQTVKETVSNGMPYGDEYLRGRGVEPGVFFDAEREDRDRVYVYFGSSSFLSMLTGSSFPDGSNFASAQKLRSALPLSGEGSPRVANLEMLRITGAFLLPSKQICDELVDLYFRYIHPLMPIMNQTEFRKAYAANTCSIFLLQAILCVAVKLSRNPLLMDKNGSTDLASNIFYQRAKSLYDGRYEENEVCLLQGMMLLSKQAFNEKNTIHMPMYFIKNAVTIAQTYGLHRSADFHPTLTANEKRARKLIWWILYVIDTLVSISIGRPQAINLDDCDVPVLTHDDFVFEGEAPEHSMPVDHMDCIISTVQIAEIMSRISRELNRPAAAHCDAKFLIQHFDLLLQRWRNNLPESLTYHSNAEIFTKHSIPKAFVNALYYKTLCLLHKSNIHDMVRASDEQYLSAGIAFQAAHSLSLVGQILLDRDELGYCYAGHAYCFFEAMTIFVHHMYDSSSIFSEIALKCYHVLEQVLLKLGQQWNSCALLGEVLFSFSKKPEYIRRLVKSFKSKISNEKSPAIPIEVPSPARSIEIDRQRPSPTSVGSWSSPIQGINAVDNKSPNSAYKQLRYAARPVFPVIPKNPRIVSPSQHSPKNPNPQVPFIQAATNPQPPPIQGPPPPWAHDFNFTTTDLSQVGPHHKSFDPHQLFPDVSQPLPADFHFNSNLEAELDTAPGPLYEGVEDEDQTEPPSSTSENPPTDLVQSSGDKTPAQLTEIRDRQPVQKTSIPLPPGFWDSIQVNFNFFTPPENVNFQWPAADSRGSDDPRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DI14</accession>
    <name>C5DI14_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0E08866p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0E08866g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553862.1">
        <property type="nucleotide sequence ID" value="XM_002553816.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DI14"/>
    <dbReference type="FunCoup" id="C5DI14">
        <property type="interactions" value="47"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DI14"/>
    <dbReference type="GeneID" id="8292023"/>
    <dbReference type="KEGG" id="lth:KLTH0E08866g"/>
    <dbReference type="eggNOG" id="KOG2382">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_53_0_1"/>
    <dbReference type="InParanoid" id="C5DI14"/>
    <dbReference type="OMA" id="KPYDYIT"/>
    <dbReference type="OrthoDB" id="8119704at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46118">
        <property type="entry name" value="PROTEIN ABHD11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46118:SF4">
        <property type="entry name" value="PROTEIN ABHD11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="34"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR23425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="310" mass="34921" checksum="EAC08D65023990F8" modified="2009-07-28" version="1">MPIKRLVHEKLPYKPVVDLAYTHFKSPDFNTQRPPVVTLHGLFGSGKTFGSVGRKLSKDLGTEVYNLDLRNHGKSEIAKPYDYLTMARDVAKFLEDKIGPQKPVSLVGFSMGGKVGLLTSLSKAVNVHKCVSIDMPPYNVDDLGTDVDQNYGKILEFCNKKIKVEKGSKGWKDKVIKIFREVPTNKNAGAALYFSQGFTQLPVNNKPFDQARDLDPYIDYKAPLLEMTDLLDQIKSWPDEVVLSREGYRIRSDTPVLFMKGLKSPMIKSTYEKLGHIFPNYEVQEFDTGHVLITEAPDEFYKSCSAFLSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>C5DI16</accession>
    <name>C5DI16_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0E08954p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0E08954g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553864.1">
        <property type="nucleotide sequence ID" value="XM_002553818.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DI16"/>
    <dbReference type="FunCoup" id="C5DI16">
        <property type="interactions" value="25"/>
    </dbReference>
    <dbReference type="GeneID" id="8292027"/>
    <dbReference type="KEGG" id="lth:KLTH0E08954g"/>
    <dbReference type="eggNOG" id="ENOG502QV7Y">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026112_0_0_1"/>
    <dbReference type="InParanoid" id="C5DI16"/>
    <dbReference type="OMA" id="CLSHCIE"/>
    <dbReference type="OrthoDB" id="66726at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16616">
        <property type="entry name" value="mRING-HC-C4C4_Asi1p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22696">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22696:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="147"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="270"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="641" mass="72488" checksum="CA037AD666529534" modified="2009-07-28" version="1">MTDLNDTTLGHFSYLNNTRAALIDENMFLAPFINYPYKIARSFYSAVLSQYKSQTGVSLATNSAHLGASTLVETAGYFFSNYAIGCFVTAMVLNRIVAMASLRSNSQVARLPLWSRFIFHACAILALGYNVILTLRPFADEKPLVDIHNYFGITYGVICLSHCIETFVTTTTNLKPMEEFDYSIFELSMHFYALTRSNPRPEDFIPDCLMALLGRLIIHSVELTNKRNRRLLLSTILNMGHLVYLGQRVYTLGTGSLPLLVRYRHFPKLLALFCTAVSLACYALACIARLNPFSSNDEAHDISDLQFHSFMNNWYATLNCTGEEEFTSTLIKLAIMICNPEQAKRFGLHRELSQLRQPGEVHKSFFVSGYNSKWQSIPQEAITDGAAKQGRTVWQRKVTAVRDLLVAVFQRLKATFGKAPTGHANESFSEEHKKNLNDFVSERNYIKFLSLAPDLNANRIQSNDLKYLLPEDDFSADYLASDDEGSEELLEEFGSEDEESELGDVPLTDLIVPPNSSMPAQMGNTNDLKWFYSMWSILSCELQHNKRLTRSQYSELNEPGILHEVMMERIAETGSSNEAKEKSERDMTCVVCKTNTRNIVLWPCKCFALCEECRVSLGLRGFKTCICCRTDVRGYSKINAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>C5DI35</accession>
    <name>C5DI35_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0E09394p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0E09394g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553883.1">
        <property type="nucleotide sequence ID" value="XM_002553837.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DI35"/>
    <dbReference type="FunCoup" id="C5DI35">
        <property type="interactions" value="971"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DI35"/>
    <dbReference type="GeneID" id="8292047"/>
    <dbReference type="KEGG" id="lth:KLTH0E09394g"/>
    <dbReference type="eggNOG" id="KOG0302">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025272_1_1_1"/>
    <dbReference type="InParanoid" id="C5DI35"/>
    <dbReference type="OMA" id="RHWKPNA"/>
    <dbReference type="OrthoDB" id="2161379at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051972">
        <property type="entry name" value="Glutamate-rich_WD_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022052">
        <property type="entry name" value="Histone-bd_RBBP4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45903">
        <property type="entry name" value="GLUTAMATE-RICH WD REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45903:SF1">
        <property type="entry name" value="GLUTAMATE-RICH WD REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12265">
        <property type="entry name" value="CAF1C_H4-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Histone-binding protein RBBP4-like N-terminal" evidence="7">
        <location>
            <begin position="119"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="328"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="424"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="191"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="38"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="91"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="195"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR23446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="522" mass="58364" checksum="1597AC95C1A5E5CC" modified="2009-07-28" version="1">MVKRSAQIEEVADTADDRVVSRKVGSSDLPPSASRDEGDIDMGEFEDQYSDEFESDGEIIQMDGDEENEDEQDGDEDFMEKNQQKAQELVNQDQAAEQNQQNAEQQLYLPHMSRPLGPDEVLEADPTVYEMLHNVNLPWPCLSLDIVPDKLGSERRNYPQSLLLTTATQASKKKDNELLLLKMSQLAKTLVKDDNNEEEDDDDEDEDQDPIVENENIPLKDTTNRLKVSPFASESPEKLTATMSENGEVYIFDLGPQVKAFEIPGYQLPKTAKKPIHTVRSHGNVEGYALDWSPLNKSGSLLTGDCSGRVYLTQRHTSKWITDKTAFSAGNNQSIEDIQFSRTEATVFASCGCDGYIRIWDTRSKKHKPAISVKASATDVNVISWNEKIGYLLASGDDNGSWGVWDLRQFSPNTAASTSPVAQYNFHKGAITSISFNPLDDSIIAVASEDNTVSLWDLSVEADDEEIKQQAAETRELQEIPPQLLFVHWQKEVKDVKWHKQIPGCLVSTGTDGLNIWKTISV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DI49</accession>
    <name>C5DI49_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">KLTH0E09702p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0E09702g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Binds 1 Mg(2+) per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TPP enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553897.1">
        <property type="nucleotide sequence ID" value="XM_002553851.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DI49"/>
    <dbReference type="FunCoup" id="C5DI49">
        <property type="interactions" value="369"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DI49"/>
    <dbReference type="GeneID" id="8292061"/>
    <dbReference type="KEGG" id="lth:KLTH0E09702g"/>
    <dbReference type="eggNOG" id="KOG1184">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013748_0_2_1"/>
    <dbReference type="InParanoid" id="C5DI49"/>
    <dbReference type="OMA" id="LRQEWVW"/>
    <dbReference type="OrthoDB" id="3970464at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004737">
        <property type="term" value="F:pyruvate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000949">
        <property type="term" value="P:aromatic amino acid family catabolic process to alcohol via Ehrlich pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02005">
        <property type="entry name" value="TPP_PDC_IPDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07038">
        <property type="entry name" value="TPP_PYR_PDC_IPDC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000019">
        <property type="entry name" value="Pyruvate decarboxylase isozyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000024">
        <property type="entry name" value="Pyruvate decarboxylase isozyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012110">
        <property type="entry name" value="PDC/IPDC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047214">
        <property type="entry name" value="TPP_PDC_IPDC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047213">
        <property type="entry name" value="TPP_PYR_PDC_IPDC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43452">
        <property type="entry name" value="PYRUVATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43452:SF30">
        <property type="entry name" value="PYRUVATE DECARBOXYLASE ISOZYME 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036565">
        <property type="entry name" value="Pyruvt_ip_decrb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme N-terminal TPP-binding" evidence="13">
        <location>
            <begin position="17"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme central" evidence="11">
        <location>
            <begin position="213"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="12">
        <location>
            <begin position="400"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="572"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="455"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="482"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="484"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR036565-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR23460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="607" mass="66991" checksum="E5958E2951DCBD44" modified="2009-07-28" version="1">MCVNYAYECGLPPEIPLAQYMLHRLRQLGIATIFGVPGGFNVPLLREICNVPQMKWAGNTNELNAAYAADGYSRIKHLACLVTTFGVGELSAVNGVAGSFAEHVGLLHFVGMPPLAAQRRRLLLHHTLGNGNYKVFHRLGSDVTQYTSVLTDTETCSKEVDKCISIAFTKQRPVYLGVPTNISDTLVSSALLNIPLDLSAHEGNTEVQEDFVNAILSAMYKCRSPAIVVDACVSRHNVVAEVRQLVELTQFPVFCTPMGKGAINEHHPRFGGTFVGSISPPQVREVVDFSDFVLVVGALLSDFNSSSFHFAYKAKNTALLFSSYARLKNAMYPDLELKTALRVLLTKLDPSKLRYRPEEVPEVIRPKIKLMSNVPLRQEWVWNQISNWFREGDIVITETGTSAFGVNQSKFPNNTRCITQALWGSVGYSVGACLGASFAAREEGNNSRVILFVGDGALQLTVQELSTMIRWGLKPIIFVMNNSGYTIDRLLHRKSNAGYHDIQAWDNLKLLPLFGALDYDTRVVKTVGDFLGLVSDPGFALNNKIKMVEVVLPPMDAPPALMDKWLVDDNDEEVGSEDTNGWQSSKRIKLDSQPSVDSLASMDSLDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIJ2</accession>
    <name>C5DIJ2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0E12936p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0E12936g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554040.1">
        <property type="nucleotide sequence ID" value="XM_002553994.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIJ2"/>
    <dbReference type="FunCoup" id="C5DIJ2">
        <property type="interactions" value="676"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIJ2"/>
    <dbReference type="GeneID" id="8292208"/>
    <dbReference type="KEGG" id="lth:KLTH0E12936g"/>
    <dbReference type="eggNOG" id="KOG0744">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028208_3_0_1"/>
    <dbReference type="InParanoid" id="C5DIJ2"/>
    <dbReference type="OMA" id="VCIEEMI"/>
    <dbReference type="OrthoDB" id="5925at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051598">
        <property type="term" value="P:meiotic recombination checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044539">
        <property type="entry name" value="Pch2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45991">
        <property type="entry name" value="PACHYTENE CHECKPOINT PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45991:SF1">
        <property type="entry name" value="PACHYTENE CHECKPOINT PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="4">
        <location>
            <begin position="293"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR23603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="544" mass="60647" checksum="ACD30DFF722A707A" modified="2009-07-28" version="1">MALIVDVQLKGPTLEVFRRLMQEMESEDNFRCCGVMSKVIIEAVKNKLRKQSFGTSDGVALDSKILLLEGQGSVEISLKPTKAQENIVKSLTKVILHQIGSKKQYNLTEGQENLLLSLCVENMQVEHVAAAAPLRGAHSSIASAIEDIFCSADPDLLHKRGAEREREHERQRERLSTSFQVNCYCCLESDRPSSNYSNIAEEFDKIDIQDSCSSGETAREAQPPHPLLAGISLSQETLSQSKISFLPSPEFEGLWESLHFEDGVKQKLSSYATISLKLASFSKQRLSQTLVSNNKILLIHGPPGTGKTTVCRALCQKLAIRCNNDPENLFKEVDYQAVVVELSCSRIFSRWFGESAKNLEKIFQDLERLLMSSLNQNRFVCLLMDEVETLASSRESLMKKNETTDGIRVVNTLLTKLDSLKRFNNLLVLATSNVASSLDAAFLDRADGVFYIGAPSEHGTCVILTSAIRELLEMGAIYATEGAALLESSEVQEAIRKVSHQCVVSLLCDKPHHPRVRESYTNYQKTAIRSHWSFIEKTSSRVHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIK4</accession>
    <name>C5DIK4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0E13222p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0E13222g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554052.1">
        <property type="nucleotide sequence ID" value="XM_002554006.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIK4"/>
    <dbReference type="FunCoup" id="C5DIK4">
        <property type="interactions" value="37"/>
    </dbReference>
    <dbReference type="GeneID" id="8292221"/>
    <dbReference type="KEGG" id="lth:KLTH0E13222g"/>
    <dbReference type="eggNOG" id="KOG0255">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008455_8_7_1"/>
    <dbReference type="InParanoid" id="C5DIK4"/>
    <dbReference type="OMA" id="FQAFGSC"/>
    <dbReference type="OrthoDB" id="3066029at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005275">
        <property type="term" value="F:amine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17323">
        <property type="entry name" value="MFS_Tpo1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1720.10">
        <property type="entry name" value="Multidrug resistance protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF21">
        <property type="entry name" value="DITYROSINE TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="107"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="141"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="175"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="202"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="235"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="263"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="357"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="400"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="443"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="468"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="507"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="532"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="110"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="47"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="51"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR23615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="566" mass="62101" checksum="9832C985898FC664" modified="2009-07-28" version="1">MPAERPLESTSPSIDNLAEWEVVEPEVVGSEYNAELVSEANEFIHRAVNEGPSNPENRENITNVDIESEKEHPSGKLKEEELKTEDADQNLSEQPDPPYTAFSQRQVLVIFIIVIFVGFLGPLSGNIYIPALPILQNVFGLSATAINATVSVFMAVFAVGPLIWASFADFGGRKILYMISLLMTVVVNVLLASVPVNVGALFTLRVVQAFASSSVMALGAGTVSDIRPPKVRGKAIAYFMLGPNMGPILAPIIAGLILMHGSYWRWLFGFTCILSGFGLLMVVVLLPETLRCIVGNGDIKWRDCEEKDNIGPTRTISVAGLLIFSDIGVVKPASVSPQFQKLYPRPPKPSLAVYWKLLKFLPVTICSITTAILFATYYAFSVTFSHFLREDYGLTNLQIGACYVCPGVALLLGSIIGGHLSDYFRKLWVKRHNGEKFPSEKRLVLQIWGLLLNICGCVGYGWSVQFHHHLVVVLVFSFFTAFGMTWCSNTSMTYLTECVPRRAAGTVAVGSFFRNLAAGISSVIVMKLCDKMGVGWCFTGLAFCDLASMAGVLYLITNGHRWKPEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIS7</accession>
    <name>C5DIS7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0E14850p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0E14850g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the NHP6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554125.1">
        <property type="nucleotide sequence ID" value="XM_002554079.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIS7"/>
    <dbReference type="FunCoup" id="C5DIS7">
        <property type="interactions" value="483"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIS7"/>
    <dbReference type="GeneID" id="8292295"/>
    <dbReference type="KEGG" id="lth:KLTH0E14850g"/>
    <dbReference type="eggNOG" id="KOG0381">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082854_10_3_1"/>
    <dbReference type="InParanoid" id="C5DIS7"/>
    <dbReference type="OMA" id="MKNMGGK"/>
    <dbReference type="OrthoDB" id="1919336at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008301">
        <property type="term" value="F:DNA binding, bending"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001195">
        <property type="term" value="P:maintenance of transcriptional fidelity during transcription elongation by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070898">
        <property type="term" value="P:RNA polymerase III preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01390">
        <property type="entry name" value="HMG-box_NHP6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000016">
        <property type="entry name" value="FACT complex subunit SSRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050342">
        <property type="entry name" value="HMGB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112">
        <property type="entry name" value="HIGH MOBILITY GROUP PROTEIN DSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112:SF22">
        <property type="entry name" value="MITOCHONDRIAL TRANSCRIPTION FACTOR A, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00886">
        <property type="entry name" value="HIGHMOBLTY12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0158">Chromosome</keyword>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="8">
        <location>
            <begin position="21"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="6">
        <location>
            <begin position="21"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="67"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="7"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00043963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR23688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="93" mass="10715" checksum="383738AE9BDE1210" modified="2009-07-28" version="1">MSAPREAKKRTTRRKKDPNAPKRALSAYMFFANENRDIVRAENPGVTFGQVGRLLGDKWKALTDEEKQPYEAKHAADKKRYESEKELYNATKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIW8</accession>
    <name>C5DIW8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Prefoldin subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0E15774g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 4">Heterohexamer of two PFD-alpha type and four PFD-beta type subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the prefoldin subunit alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554166.1">
        <property type="nucleotide sequence ID" value="XM_002554120.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIW8"/>
    <dbReference type="FunCoup" id="C5DIW8">
        <property type="interactions" value="1052"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIW8"/>
    <dbReference type="GeneID" id="8292337"/>
    <dbReference type="KEGG" id="lth:KLTH0E15774g"/>
    <dbReference type="eggNOG" id="KOG3313">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083737_0_0_1"/>
    <dbReference type="InParanoid" id="C5DIW8"/>
    <dbReference type="OMA" id="YNWDVAQ"/>
    <dbReference type="OrthoDB" id="6375174at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007021">
        <property type="term" value="P:tubulin complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23156">
        <property type="entry name" value="Prefoldin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.370:FF:000001">
        <property type="entry name" value="Prefoldin subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016655">
        <property type="entry name" value="PFD3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004127">
        <property type="entry name" value="Prefoldin_subunit_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12409">
        <property type="entry name" value="PREFOLDIN SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12409:SF0">
        <property type="entry name" value="PREFOLDIN SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02996">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016396">
        <property type="entry name" value="Prefoldin_subunit_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="131"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR016396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR23729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="192" mass="22447" checksum="21F1552EB216DB63" modified="2009-07-28" version="1">MEALFNSTKTNPRGIPEAPFVERVEDFLKDPNDFDLCFNKFQERLSKYKFMQESKLVTVRQLRQKIPDIENTLNMCRALEKKKESGSNMETNYQLNETLYTKAEIDTSEDLKVGLWLGADVMLEYPVEEAITLLEGKLSDAKQNLEVNQEDVEFLRENITTMEVNCARLYNWDVEKRQQLKHAQQGTENLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJ28</accession>
    <name>C5DJ28_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0F12980p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0F12980g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554754.1">
        <property type="nucleotide sequence ID" value="XM_002554708.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJ28"/>
    <dbReference type="GeneID" id="8292977"/>
    <dbReference type="KEGG" id="lth:KLTH0F12980g"/>
    <dbReference type="HOGENOM" id="CLU_2333964_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJ28"/>
    <dbReference type="OrthoDB" id="5398685at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="98" mass="11057" checksum="81D5D4C96F0E23E5" modified="2009-07-28" version="1">MWKVITNKLRRGSTKSMVHTPEEKQDQNFSNSAGFLETFKQLQEGEKTAEKLESMLDKLEGKLEELLSQADAVIQEPHNKPANSKPESRIDAQGKTSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJD6</accession>
    <name>C5DJD6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0F15576p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0F15576g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the complex I LYR family. SDHAF1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554862.1">
        <property type="nucleotide sequence ID" value="XM_002554816.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJD6"/>
    <dbReference type="FunCoup" id="C5DJD6">
        <property type="interactions" value="102"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJD6"/>
    <dbReference type="GeneID" id="8293093"/>
    <dbReference type="KEGG" id="lth:KLTH0F15576g"/>
    <dbReference type="eggNOG" id="KOG4620">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_154777_1_1_1"/>
    <dbReference type="InParanoid" id="C5DJD6"/>
    <dbReference type="OMA" id="VEMYSSP"/>
    <dbReference type="OrthoDB" id="273010at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034553">
        <property type="term" value="P:mitochondrial respiratory chain complex II assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20268">
        <property type="entry name" value="Complex1_LYR_SDHAF1_LYRM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008011">
        <property type="entry name" value="Complex1_LYR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045295">
        <property type="entry name" value="Complex1_LYR_SDHAF1_LYRM8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052687">
        <property type="entry name" value="SDHAF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47046">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE ASSEMBLY FACTOR 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47046:SF1">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE ASSEMBLY FACTOR 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05347">
        <property type="entry name" value="Complex1_LYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Complex 1 LYR protein" evidence="5">
        <location>
            <begin position="10"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR24425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="79" mass="9585" checksum="46C1331F68D20733" modified="2009-07-28" version="1">MAKRLSGLQREVLQMYRQCIRAAHDKPKANQPNFVTHIRKEFRQYQDLPRKDFSTIEHLLRVGHRRLEMYSQPEVKDIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJI3</accession>
    <name>C5DJI3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0F16654p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0F16654g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554909.1">
        <property type="nucleotide sequence ID" value="XM_002554863.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJI3"/>
    <dbReference type="FunCoup" id="C5DJI3">
        <property type="interactions" value="29"/>
    </dbReference>
    <dbReference type="GeneID" id="8293142"/>
    <dbReference type="KEGG" id="lth:KLTH0F16654g"/>
    <dbReference type="eggNOG" id="ENOG502QR4N">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019068_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJI3"/>
    <dbReference type="OMA" id="WWNILER"/>
    <dbReference type="OrthoDB" id="289721at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031403">
        <property type="entry name" value="Sbe2/Sbe22_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053949">
        <property type="entry name" value="SBE2/SBE22_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053948">
        <property type="entry name" value="SBE2/SBE22_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17076">
        <property type="entry name" value="SBE2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22874">
        <property type="entry name" value="SBE2_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22876">
        <property type="entry name" value="SBE2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SBE2/SBE22 N-terminal" evidence="9">
        <location>
            <begin position="49"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="SBE2/SBE22 middle" evidence="8">
        <location>
            <begin position="336"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="Sbe2/Sbe22 C-terminal" evidence="7">
        <location>
            <begin position="463"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="71"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="200"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="298"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="40"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="71"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="91"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="114"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="208"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="269"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="312"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF22874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF22876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR24472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="773" mass="87542" checksum="703243D4BF1356DC" modified="2009-07-28" version="1">MSLHSAHTLDTLQEEAGTPRSKHNVSTQSPALRVKTGLYPKPSSGSSNGSKTIGLGIARRPSDNLLMNLAQEQQRQQGQPSAVVPPPSRKQLVSSNDRPLSNESILTQNSALFSSNPNSTASSSSDNSRGSSFIDDLSTDSVKRIGDETTFAETDGESTIDLTSTLGSEVSNSTHQSLVSAPPKTRYVFTNSSLTRSQVSFSASSPSPLKNNISPNAAPNKLFSKSTSALSQQQVLTPSQRYRLRKEKNKTALQNSIKQKEKFYEEQESPNTGGYTNESDIDNSLIWNIPVASNSTNSFLMSLKPPPKTTRSKSTGGFSRSSSQHSNLSESNSGYLDYHEMPPSPVPGLHKTSDFEYLQKTSESLSTIYQHSSNRFSQSKLWERNSSMEFLPMQFKTASDEGMEDLRLVSDDKVRLCSPSRPTWLPPKVESERKSHEEQINKTMSMASIDLLDKSREREERLVRDETNRQKIVLLIDRGLTRQSSLHNLKKIIWDTQLMPEVRAQVYDKILQSESRLISKLYIEPLRDISSVMDKMHFPRSKQTEIERLIARIPAIEELKDVEDLKHLLKLKSISSKGLLPGDELLFFHLLIDGSFSNIEQIWEMVNLIQLTCFNEVTAEKFDARAVGSRGVVAHYLCKNDEFKNEFNSRCLNIGTWWNVLQRVNHSLFMWCLDVIVAHNSQCFKASSIQKEKFVDKPWELYMSQHVVVNYKILVSMMLSVLLNYHFGFNDLISLADLQDKEFRIPCPMDTLLDESSVYEVFVKKWRHYYKKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJK3</accession>
    <name>C5DJK3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sphingoid long-chain base transporter RSB1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0F17138g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the ATP-dependent translocation of sphingoid long-chain bases (LCBs) from the cytoplasmic site toward the extracytoplasmic side of the membrane (flip-flop). Involved in the establishment of the functional lipid asymmetry of the plasma membrane. Regulates intracellular levels of LCBs, sphingolipid precursors that are growth inhibitory at increased levels.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the lipid-translocating exporter (LTE) (TC 9.A.26.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554929.1">
        <property type="nucleotide sequence ID" value="XM_002554883.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJK3"/>
    <dbReference type="FunCoup" id="C5DJK3">
        <property type="interactions" value="59"/>
    </dbReference>
    <dbReference type="GeneID" id="8293163"/>
    <dbReference type="KEGG" id="lth:KLTH0F17138g"/>
    <dbReference type="eggNOG" id="ENOG502QU4U">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033465_6_3_1"/>
    <dbReference type="InParanoid" id="C5DJK3"/>
    <dbReference type="OMA" id="IMGGIYY"/>
    <dbReference type="OrthoDB" id="3358017at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007568">
        <property type="entry name" value="RTA1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31465">
        <property type="entry name" value="PROTEIN RTA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31465:SF9">
        <property type="entry name" value="SPHINGOID LONG-CHAIN BASE TRANSPORTER RSB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04479">
        <property type="entry name" value="RTA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="75"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="188"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="262"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="298"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="342"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="351"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="361"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR24492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="370" mass="40976" checksum="A42C7F96DD51307C" modified="2009-07-28" version="1">MLATPVLRAASASAASAASAASSAALSAALSSASSTEYSNYSGMVPNLAFNVCMAVIFGLLLIMQVGIGIWTKQWWFLVSFTFTCILEVLGYAGRASGHFDVYNVDTFLLQFITLTIAPVFTMAGIYYQLAKLIEVFGHQFALIKNPMLYSYIFMGCDIISLIVQAVGGGMAGSAAADEKDPANGDHVFVAGLAFQVASMSVFLMLWFHLVYQIFVVTRKRHTGAKRASLSLFHVSQEEIDYMYRPKFEFLRLHPKRWVFTYFPWILTAASLLIFVRCIYRVIELAEGWSGNLITHEIYFIILDALMMSLATTLLTVFHPGFVFKGRTTSIPIQNVRKAVAKDGAENATDSLEDDNDNDNDSWRANKEEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJT1</accession>
    <name>C5DJT1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein uL23</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">60S ribosomal protein L25</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0F18898g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the universal ribosomal protein uL23 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555007.1">
        <property type="nucleotide sequence ID" value="XM_002554961.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJT1"/>
    <dbReference type="FunCoup" id="C5DJT1">
        <property type="interactions" value="883"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJT1"/>
    <dbReference type="GeneID" id="8293243"/>
    <dbReference type="KEGG" id="lth:KLTH0F18898g"/>
    <dbReference type="eggNOG" id="KOG1751">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037562_0_1_1"/>
    <dbReference type="InParanoid" id="C5DJT1"/>
    <dbReference type="OMA" id="RLDHHKV"/>
    <dbReference type="OrthoDB" id="1267328at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000035">
        <property type="entry name" value="60S ribosomal protein L23a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01369_A">
        <property type="entry name" value="Ribosomal_uL23_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013025">
        <property type="entry name" value="Ribosomal_uL23-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_uL23/eL15/eS24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001014">
        <property type="entry name" value="Ribosomal_uL23_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005633">
        <property type="entry name" value="Ribosomal_uL23_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011118">
        <property type="entry name" value="PRK14548.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11620">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L23A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00276">
        <property type="entry name" value="Ribosomal_L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03939">
        <property type="entry name" value="Ribosomal_L23eN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="Ribosomal proteins S24e, L23 and L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00050">
        <property type="entry name" value="RIBOSOMAL_L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL23 N-terminal" evidence="9">
        <location>
            <begin position="4"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00075004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR24570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="142" mass="15765" checksum="33DDB3C7B02B0544" modified="2009-07-28" version="1">MAPSTKATAAKKAVVKGSNGKKALKVRTSATFRLPKTLKLARRPAYASKSVPHYNRLDSYKVIEQPITSETAMKKVEDGNTLVFQVSIKSNKHQIKKAVKDLYEVDVRSVNTLVRPNGTKKAYVRLTADYDALDIANRIGYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJV4</accession>
    <name>C5DJV4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">KLTH0F19404p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0F19404g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555030.1">
        <property type="nucleotide sequence ID" value="XM_002554984.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJV4"/>
    <dbReference type="FunCoup" id="C5DJV4">
        <property type="interactions" value="28"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJV4"/>
    <dbReference type="GeneID" id="8293266"/>
    <dbReference type="KEGG" id="lth:KLTH0F19404g"/>
    <dbReference type="eggNOG" id="KOG1397">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001583_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJV4"/>
    <dbReference type="OMA" id="PQWDMIT"/>
    <dbReference type="OrthoDB" id="16982at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015369">
        <property type="term" value="F:calcium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.30:FF:000014">
        <property type="entry name" value="Cation/H+ exchanger protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004713">
        <property type="entry name" value="CaH_exchang"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005185">
        <property type="entry name" value="YccF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31503">
        <property type="entry name" value="VACUOLAR CALCIUM ION TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31503:SF10">
        <property type="entry name" value="VNX1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03733">
        <property type="entry name" value="YccF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="169"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="293"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="380"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="404"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="439"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="475"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="507"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="569"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="636"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="673"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="705"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="746"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="777"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="domain" description="Inner membrane component" evidence="12">
        <location>
            <begin position="165"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="11">
        <location>
            <begin position="410"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="11">
        <location>
            <begin position="634"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="81"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF03733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR24593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="802" mass="90098" checksum="4FA516C1BCC57DAB" modified="2009-07-28" version="1">MPKERPLKPVSSNQEPLRRVFSVDDGIQEIEDDIRYLEGLQEGLKFAAANRKTSGNVNTPPPIATRPNLVSHKTSDLSIIQDTDRLAGGDASTDAAPGDDEERGSVASRESYTLRERQDAINETHPFGIRIWKPALYKKHRSVQRAADEDIHETKYKRISWKVHVGNNIWAATVGIFLLAFLSSCGLLVCLLGLFTQPSVEYASVLFRLGHYLFWPFGKVVLIISDQQYLHEDQDEGISAQQFYNWVTGHRNRLYFHNSQAGSGNYGSTGSDNNNTDRHRRLFGRGEWSFGRFWFYLVFYVFVQPVSLLLAFLTWLGVFTIPMSNVLWNLMYHCRRHPLALEFKTLRHSVNGIKDKNVLLCTFRCAGSHYYKYTVDGTNVIVMNLGAIVVFTIFQFYVLQNHIFPMNGSLIFCLCLLSIIPLAFYIGQAVASISAQTSMGVGAVINAFFSTVVEVFLYCVALQQEKGLLVEGSMIGSILGAVLLLPGLSMCGGAIKRKTQRYNPASAGVSSAMLIFSMIVMFVPTIIFQIYGGYLMDCPPEHQEKPSRCYFRQPPLKFDGLYSHVIKPMSIFCALILFMAYIIGLWFTLRTHAAAIWQVPEKQSLNASSQLGFIPEEEESEGGHDGPNWSRKKSTCILLGATLLYAVIAEILVDCVDNVLQQFPSLDPKFLGLTVFALVPNSTEFLNAISFAIHGNVALSMEIGTAYALQVCLLQIPALVVFSIIYTMNYPNDSIVIRDQMFSLVFPRWDLLACMVSVFLFTYLYAEGKSNYFKGSILILLYCVMTLGFYFQGAIDNNNWDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>C5DK09</accession>
    <name>C5DK09_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0F00880p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0F00880g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0057 (PMP3) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554247.1">
        <property type="nucleotide sequence ID" value="XM_002554201.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DK09"/>
    <dbReference type="FunCoup" id="C5DK09">
        <property type="interactions" value="733"/>
    </dbReference>
    <dbReference type="GeneID" id="8292438"/>
    <dbReference type="KEGG" id="lth:KLTH0F00880g"/>
    <dbReference type="eggNOG" id="KOG1773">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107649_5_0_1"/>
    <dbReference type="InParanoid" id="C5DK09"/>
    <dbReference type="OMA" id="HALWVIS"/>
    <dbReference type="OrthoDB" id="2802411at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000612">
        <property type="entry name" value="PMP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21659">
        <property type="entry name" value="HYDROPHOBIC PROTEIN RCI2 LOW TEMPERATURE AND SALT RESPONSIVE PROTEIN LTI6 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21659:SF112">
        <property type="entry name" value="PROTEIN SNA2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01679">
        <property type="entry name" value="Pmp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01309">
        <property type="entry name" value="UPF0057"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR23810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="76" mass="8432" checksum="0E31534C88FCDBDB" modified="2009-07-28" version="1">MHARDWFLVFLSIFVPPIAVGLKRGFFTKDTLLNLLLFLLGYLPGLIHALYVISKHPYPSVGGSLTQPNDGYGSLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKA1</accession>
    <name>C5DKA1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0F02948p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0F02948g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the THOC3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554339.1">
        <property type="nucleotide sequence ID" value="XM_002554293.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKA1"/>
    <dbReference type="FunCoup" id="C5DKA1">
        <property type="interactions" value="803"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKA1"/>
    <dbReference type="GeneID" id="8292531"/>
    <dbReference type="KEGG" id="lth:KLTH0F02948g"/>
    <dbReference type="eggNOG" id="KOG4155">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_672809_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKA1"/>
    <dbReference type="OMA" id="WNADGRH"/>
    <dbReference type="OrthoDB" id="340259at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000445">
        <property type="term" value="C:THO complex part of transcription export complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040132">
        <property type="entry name" value="Tex1/THOC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22839:SF0">
        <property type="entry name" value="THO COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22839">
        <property type="entry name" value="THO COMPLEX SUBUNIT 3 THO3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="45"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="229"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="356"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="389"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00046343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR23902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="399" mass="44670" checksum="339A5C419281450F" modified="2009-07-28" version="1">MPNLDSAQAVEKYVSFYPECQKEVMADDHYSQLTLKNSSRFAHASVEEGNRVVALEFNTRGTYLAYSREDGSLNLWKVKSDRAQTYSVVREVHGIGRKISSISWHPVTHFLLATVGGTSQVNIWDASTGVLIKSFETSTETTNLKCCYDASGRWLGVLSSTNEFYMFDADQNYNLASVSELGSECAESGDQVTALCWSPDSKHLYAGRRSGKLGVFAVRNPGLEEQLQVSGHTSAIYCLRIDPQSRFLIAGGDDAVCSVWDASNMCCETVISDFDTPIIDMDLSRDALALALCSETQTRVYSVSSGACIFQLDQKNRVDDQKFRFYPEKMSFVTIAENECVVKYYVPKITEPPLQKEWAEADKRKRPARKVSSLGNLGAARRDDEPPRRGRFSKRPLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKI8</accession>
    <name>C5DKI8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0F05060p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0F05060g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the PIR protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554426.1">
        <property type="nucleotide sequence ID" value="XM_002554380.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKI8"/>
    <dbReference type="GeneID" id="8292624"/>
    <dbReference type="KEGG" id="lth:KLTH0F05060g"/>
    <dbReference type="eggNOG" id="ENOG502RKR1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039662_2_0_1"/>
    <dbReference type="InParanoid" id="C5DKI8"/>
    <dbReference type="OMA" id="AVSQIWD"/>
    <dbReference type="OrthoDB" id="5415592at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005199">
        <property type="term" value="F:structural constituent of cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054508">
        <property type="entry name" value="PIR1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051153">
        <property type="entry name" value="Yeast_CWMannoprotein_PIR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000420">
        <property type="entry name" value="Yeast_PIR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47254">
        <property type="entry name" value="CELL WALL MANNOPROTEIN CIS3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47254:SF1">
        <property type="entry name" value="CELL WALL MANNOPROTEIN CIS3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00399">
        <property type="entry name" value="PIR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22799">
        <property type="entry name" value="PIR1-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00929">
        <property type="entry name" value="PIR_REPEAT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50256">
        <property type="entry name" value="PIR_REPEAT_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0134">Cell wall</keyword>
    <keyword evidence="4" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Cell wall mannoprotein PIR1-like C-terminal" evidence="9">
        <location>
            <begin position="163"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="79"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="83"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="117"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF22799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR23989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="246" mass="25202" checksum="EC7A08F8751600EB" modified="2009-07-28" version="1">MQIRNIIASAIASSCVVSAEEVNSSSWTTLTPSKTYQGGATDYADSFGIAVKPVSGTSVTASAASATGKSKRDAVSQIGDGQIQGTTSTALPVSTVSTPEPESTAAASQVSDGQIQATTSTASKETTTLSSASASASSDVDDDIKVVSCKVNGTLEMTLSNSILKDGKGRIGAIVANRQFQFDGPPPQAGSIYAAGWSITTDGNLAIGETEVFYQCLSGTFYNLYDESIGAQCSPVHLEVVKLIDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKM0</accession>
    <name>C5DKM0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0F05808p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0F05808g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554458.1">
        <property type="nucleotide sequence ID" value="XM_002554412.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKM0"/>
    <dbReference type="FunCoup" id="C5DKM0">
        <property type="interactions" value="620"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKM0"/>
    <dbReference type="GeneID" id="8292656"/>
    <dbReference type="KEGG" id="lth:KLTH0F05808g"/>
    <dbReference type="eggNOG" id="KOG2641">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012923_4_0_1"/>
    <dbReference type="InParanoid" id="C5DKM0"/>
    <dbReference type="OMA" id="YNLALFW"/>
    <dbReference type="OrthoDB" id="5348404at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005178">
        <property type="entry name" value="Ostalpha/TMEM184C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23423">
        <property type="entry name" value="ORGANIC SOLUTE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03619">
        <property type="entry name" value="Solute_trans_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01417">
        <property type="entry name" value="Solute_trans_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="49"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="79"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="169"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="244"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR24021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="413" mass="48280" checksum="EAC27C1E1CAD63A9" modified="2009-07-28" version="1">MNYYIPRWWFLLCVLSSVVSITISAFSISQQLLNYRKPYEQRLVIRIQLMVPLFCVTCLLACVIPQWSEVWINPIREIYEAFVIYTFFSLLTLILGGERKIITELSMGKPPMRHPIPVLGSFISAVDMSDPNDFLSIKRGILQYVWIKPFYCLGNIICMKYEWKREFWSLFWTIVYNVSASLSLYNLALFWKCLYGDLQKYNPWSKFLCVKLIIFASYWQEILISVLSKYGVIRHDSDVDYGYVYQNAVLCVEMIGFAIGHLYAFIWTTYDSKRIPEGARLRFYSAVKDCFGFGDLIWDFRVTFLGNLYSYRNFNSAEAVVAHLDARSRLNRLQAGLRYTDGGRENYWVNEGDQSPASSRYGSVGEDDSWTEISPTGFVPEDPNYPVKWDVDAHKYSRDIERLRQNTRRAYPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKN5</accession>
    <name>C5DKN5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0F06160p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0F06160g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Bud neck</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the nucleosome assembly protein (NAP) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554473.1">
        <property type="nucleotide sequence ID" value="XM_002554427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKN5"/>
    <dbReference type="SMR" id="C5DKN5"/>
    <dbReference type="FunCoup" id="C5DKN5">
        <property type="interactions" value="1840"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKN5"/>
    <dbReference type="GeneID" id="8292672"/>
    <dbReference type="KEGG" id="lth:KLTH0F06160g"/>
    <dbReference type="eggNOG" id="KOG1507">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038841_1_0_1"/>
    <dbReference type="InParanoid" id="C5DKN5"/>
    <dbReference type="OMA" id="YSGDFMY"/>
    <dbReference type="OrthoDB" id="27325at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005940">
        <property type="term" value="C:septin ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1120.90:FF:000003">
        <property type="entry name" value="Nucleosome assembly protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1500:FF:000001">
        <property type="entry name" value="Nucleosome assembly protein 1-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.90">
        <property type="entry name" value="Nucleosome assembly protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037231">
        <property type="entry name" value="NAP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002164">
        <property type="entry name" value="NAP_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11875">
        <property type="entry name" value="TESTIS-SPECIFIC Y-ENCODED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00956">
        <property type="entry name" value="NAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143113">
        <property type="entry name" value="NAP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="136"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="311"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="375"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="18"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="156"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="324"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="375"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR24036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="432" mass="48547" checksum="71425B15157FB670" modified="2009-07-28" version="1">MSKPIKTTSSKIDAAPTPHNTPSSVLGSSYLKNGNPARNIPQTINEEEINSALANNPQLLASIQDKLGDLVGQDSGYVASLPGAVRDRIYALKKLQRDLFEMEKEFQLEMFALEEKYLKKYAPVHQRRFEIVTGKQEPTAQEIEAGKDEGDQLDTLAEEPEEDEEGADNAAEDLKGIPSFWLTAFENLPIMSQTVTSADAEVLEFLTDVKLEYLTEGKPGFKLVFEFDPENPFFSNSQLVKTYYYQSELGYSGDFIYDHAEGCKIDWTDNESNVTISVEKRKQRNKTTKQVRTIEKITPVESFFNFFDPPKLPAKAKDASGSGDENEDDDEDEEDDEEVAELESRLALDYAIGEEFKDKLIPRAVDWFTGAAIEFEYDADQPDDEEFDSELDDDEEDDDEDDDEDGDDDGSENDFASAAPGTKEQPPECKQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKQ2</accession>
    <name>C5DKQ2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0F06578g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554490.1">
        <property type="nucleotide sequence ID" value="XM_002554444.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKQ2"/>
    <dbReference type="FunCoup" id="C5DKQ2">
        <property type="interactions" value="247"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKQ2"/>
    <dbReference type="GeneID" id="8292691"/>
    <dbReference type="KEGG" id="lth:KLTH0F06578g"/>
    <dbReference type="eggNOG" id="KOG0590">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_82_3_1"/>
    <dbReference type="InParanoid" id="C5DKQ2"/>
    <dbReference type="OMA" id="EIACYFK"/>
    <dbReference type="OrthoDB" id="6513151at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13994">
        <property type="entry name" value="STKc_HAL4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343">
        <property type="entry name" value="SERINE/THREONINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343:SF515">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE RTK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="221"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="50"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="116"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="181"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="552"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="68"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="116"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="577"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR24053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="596" mass="66506" checksum="1C668CA8CD0A5044" modified="2009-07-28" version="1">MPRQEHLSATPSANEASSIKSNGSTSSLTSHFFGKKHGFGLGKLFKGNSHSSNNESGKELLGSILSPKHSNSSTKSQDSYQRLRSAVPMMDHPQLSHAEAKHLHNPHNYVSFGPSKNNSAYSSQTHVANSVSPSAPHMHPAQIMQRQIEEQQRRNTPPKLAHEKPKRKVLHLKRFFKKIHGEDASPQKAQTPVAVSGPDSKSTAKTLYEADSARELIEKYGIPGKMIGEGASGSVSVVKSMDGKLFAVKRFRARGTKESQIEYSKKVTAEFCIGSTLHHCNIIETLDLLQEGPNFLVVMEYCPYDFFTLVMSDLMTKHEIACYFKQICNGVAYLHKTGLAHRDLKLDNCVVTSQGILKLIDFGSAVVFQYPYEQEILNARGIVGSDPYLAPELLTYPSYDPRPVDVWSVAIMFYCMTLRRFPWKAPRDKYQSFRLFCEETDNEKDPTRGAYRLLKLLPRHSRHIIGQMLQLDPRKRILMNEVMADPWVVSIESCELDDKGELKSAAKSHKHHLITEEELTELNLQRKEEARLAKLHKEAGESTDVPEVLGKEAAAVPEKDGHPQNVGVSTHLDDGTAASQKPTGENVTQAVEAKTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKQ3</accession>
    <name>C5DKQ3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">KLTH0F06600p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0F06600g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_002554445.1"/>
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    <dbReference type="AlphaFoldDB" id="C5DKQ3"/>
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        <property type="interactions" value="99"/>
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    <dbReference type="STRING" id="559295.C5DKQ3"/>
    <dbReference type="GeneID" id="8292692"/>
    <dbReference type="KEGG" id="lth:KLTH0F06600g"/>
    <dbReference type="eggNOG" id="KOG2118">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="HOGENOM" id="CLU_011310_3_1_1"/>
    <dbReference type="InParanoid" id="C5DKQ3"/>
    <dbReference type="OMA" id="DIHQHIM"/>
    <dbReference type="OrthoDB" id="5353557at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
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        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0030026">
        <property type="term" value="P:intracellular manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010960">
        <property type="term" value="P:magnesium ion homeostasis"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04590">
        <property type="entry name" value="CBS_pair_CorC_HlyC_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DUF21 and CBS domain protein"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="CBS-domain"/>
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        <property type="entry name" value="ACDP"/>
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        <property type="entry name" value="CBS_dom"/>
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        <property type="entry name" value="CBS_dom_sf"/>
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    <dbReference type="InterPro" id="IPR002550">
        <property type="entry name" value="CNNM"/>
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    <dbReference type="InterPro" id="IPR044751">
        <property type="entry name" value="Ion_transp-like_CBS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12064">
        <property type="entry name" value="METAL TRANSPORTER CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12064:SF97">
        <property type="entry name" value="METAL TRANSPORTER CNNM-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01595">
        <property type="entry name" value="CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51846">
        <property type="entry name" value="CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0129">CBS domain</keyword>
    <keyword evidence="5 7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 7 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="78"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="164"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="191"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="CNNM transmembrane" evidence="11">
        <location>
            <begin position="74"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="10">
        <location>
            <begin position="338"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="441"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="499"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="550"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="646"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="515"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="552"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="681"/>
            <end position="704"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
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    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR24054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="728" mass="78886" checksum="B9746472FD900838" modified="2009-07-28" version="1">MSLHTHAFRSKDFMASAEVFTSTVPAQAPRVELHRFMASPGTLLSVRRTVWLFSLIHAISGRPIAHVAENDEIQEARFATFLSISVLLVLLGGVFAGLTLGLMGQDEVYLKVISTSGTPREQRAATKVLNLISKGKHWLLVTLLLSNVITNETLPIVLDRCLGGGWQAVVSSTVLIVIFGEVIPQSICVRYGLEVGAFFCPFVLVLMYAMYPVAYPVALLLDWILGEDHGTMYKKSGLKTLVTLHRTMGVDRLTNDEVTIISAVLDLKEKKVSEIMTPIVNVFTMSADATLDEKTVGEIFNSGFSRIPIHLPGEKNNFIGMLLVRVLISYDPDDCLPVSHFPLATLPETSPDTSCLNILNYFQEGKSHMCVVSQEPGSSSGALGVLTLEDVIEELIGEEIVDESDVFVDIHQRIMREQPGPLSKRHITSYLHSLYKVTQRHSVDNTGENSPLLEESPDPVQKPKVAHHVSGVVPSNLASNPLHTQNPHVKVKRQIDIPGHHLKLSSPGYGGTDVSPKSTRSGSINSAQHSDTSILREHLGAAKKAMAHTLETGESTQAVTSKQSTPAEDVGNLSTISHENAASTSNSTSDRQPSEPASETQLISSSYRPSIKGIVESVVTVKGVPKTIIAPAKDWDESQGVVINESNNVHESGGSGDDNMAHSLSSRPSKSRRGSIFSLFRQGSSDGGSSASIRGRSAQASSSSDRVEPETLLSDEAYSLRNTSPRTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKQ5</accession>
    <name>C5DKQ5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Argininosuccinate synthase</fullName>
            <ecNumber evidence="3">6.3.4.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Citrulline--aspartate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0F06644g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the argininosuccinate synthase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.5" evidence="3"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554493.1">
        <property type="nucleotide sequence ID" value="XM_002554447.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKQ5"/>
    <dbReference type="FunCoup" id="C5DKQ5">
        <property type="interactions" value="1026"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKQ5"/>
    <dbReference type="GeneID" id="8292694"/>
    <dbReference type="KEGG" id="lth:KLTH0F06644g"/>
    <dbReference type="eggNOG" id="KOG1706">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032784_4_2_1"/>
    <dbReference type="InParanoid" id="C5DKQ5"/>
    <dbReference type="OMA" id="WRWTVSP"/>
    <dbReference type="OrthoDB" id="1688907at2759"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00113"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004055">
        <property type="term" value="F:argininosuccinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000053">
        <property type="term" value="P:argininosuccinate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000050">
        <property type="term" value="P:urea cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01999">
        <property type="entry name" value="ASS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000019">
        <property type="entry name" value="Argininosuccinate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1260.10:FF:000003">
        <property type="entry name" value="Argininosuccinate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1260.10">
        <property type="entry name" value="Argininosuccinate synthetase, chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00005">
        <property type="entry name" value="Arg_succ_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048268">
        <property type="entry name" value="Arginosuc_syn_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048267">
        <property type="entry name" value="Arginosuc_syn_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001518">
        <property type="entry name" value="Arginosuc_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018223">
        <property type="entry name" value="Arginosuc_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023434">
        <property type="entry name" value="Arginosuc_synth_type_1_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024074">
        <property type="entry name" value="AS_cat/multimer_dom_body"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00032">
        <property type="entry name" value="argG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001770">
        <property type="entry name" value="PRK00509.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11587">
        <property type="entry name" value="ARGININOSUCCINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11587:SF2">
        <property type="entry name" value="ARGININOSUCCINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20979">
        <property type="entry name" value="Arginosuc_syn_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00764">
        <property type="entry name" value="Arginosuc_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69864">
        <property type="entry name" value="Argininosuccinate synthetase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00564">
        <property type="entry name" value="ARGININOSUCCIN_SYN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00565">
        <property type="entry name" value="ARGININOSUCCIN_SYN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arginosuccinate synthase-like N-terminal" evidence="12">
        <location>
            <begin position="5"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="Arginosuccinate synthase C-terminal" evidence="13">
        <location>
            <begin position="178"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF20979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR24056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="418" mass="46493" checksum="8A36E7862AD0055A" modified="2009-07-28" version="1">MSKGKVCLAYSGGLDTSIILAWLLEEGYEVVAFMANVGQEEDFDAAEKKAMAIGATKYVCVDCREDFVKDVLFPAVQVNAVYEDVYLMGTSLARPVIAKAQIDVAEAEGCFAVAHGCTGKGNDQIRFELGFYALKPDVKVIAPWRDPAFFERFAGRKDLLDYAAEKGIPVTQTKAKPWSTDENQAHISYEAGILEDPDTTPPKGMWKLIVDPQDAPDKPEDLTIVFEKGIPVKISFTEDGEAKEVTKPLDVFLTASNLARRNGVGRIDIVEDRYINLKSRGCYEQAPLTVLRRAHVDLEGLVLDKEVRHLRDQFVTPTYSRLMYNGSYFTPEFDFIKSMIAPSQTHVNGQVRLRLYKGNVIVLGRSSETENLYDPTESSMDELTGFLPTDTTGFIAIQAIRVKKYGEAKKAKGEKLTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DL11</accession>
    <name>C5DL11_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Kinase</fullName>
            <ecNumber evidence="4">2.7.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0F09086g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the inositol phosphokinase (IPK) family.</text>
    </comment>
    <dbReference type="EC" id="2.7.-.-" evidence="4"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554599.1">
        <property type="nucleotide sequence ID" value="XM_002554553.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DL11"/>
    <dbReference type="FunCoup" id="C5DL11">
        <property type="interactions" value="79"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DL11"/>
    <dbReference type="GeneID" id="8292805"/>
    <dbReference type="KEGG" id="lth:KLTH0F09086g"/>
    <dbReference type="eggNOG" id="KOG1620">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042569_3_0_1"/>
    <dbReference type="InParanoid" id="C5DL11"/>
    <dbReference type="OMA" id="FRICGMK"/>
    <dbReference type="OrthoDB" id="338650at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000824">
        <property type="term" value="F:inositol-1,4,5,6-tetrakisphosphate 3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008440">
        <property type="term" value="F:inositol-1,4,5-trisphosphate 3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032958">
        <property type="term" value="P:inositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.160">
        <property type="entry name" value="Inositol polyphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005522">
        <property type="entry name" value="IPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038286">
        <property type="entry name" value="IPK_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12400">
        <property type="entry name" value="INOSITOL POLYPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12400:SF103">
        <property type="entry name" value="INOSITOL POLYPHOSPHATE MULTIKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03770">
        <property type="entry name" value="IPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR24162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="334" mass="37806" checksum="3BC53F0A44F545DE" modified="2009-07-28" version="1">MSGSYRKAKHQAAGHDGNFTDEGESLFFKPTTAQEVEFYQDIQRRYTDSTSEDLKLDAWMPVFLGTLELGVTGRARESDDSEVQSAITAHANLTQVEPELVPPNKPIIVLENLLRGYRTPNILDVKLGKTLHDEFASEDKKLRLRKVSQETTSGSLGLRICGMKIQESAAASTLDDCTYARESDGYISINKFYGRELNVNNVKDGFKLFFDSNSLSCDRRKALTETFLQRIQLLYNTLLEEEVRMISASLLLVYEADSDRWDELQDEDTLLRTDFFQDFSDDEEEEEPTAPLSSLSLIDFAHSKVTKGQGYDENVICGVESLIDIFDEMRLPSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLB3</accession>
    <name>C5DLB3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0F11572g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the PP2C family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="3"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554701.1">
        <property type="nucleotide sequence ID" value="XM_002554655.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLB3"/>
    <dbReference type="FunCoup" id="C5DLB3">
        <property type="interactions" value="171"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLB3"/>
    <dbReference type="GeneID" id="8292914"/>
    <dbReference type="KEGG" id="lth:KLTH0F11572g"/>
    <dbReference type="eggNOG" id="KOG0698">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013173_4_2_1"/>
    <dbReference type="InParanoid" id="C5DLB3"/>
    <dbReference type="OMA" id="MQGYRMT"/>
    <dbReference type="OrthoDB" id="10264738at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000087">
        <property type="entry name" value="Type 2C protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832:SF803">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0464">Manganese</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="10">
        <location>
            <begin position="50"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR24264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="395" mass="44297" checksum="93223FB6B7917DF0" modified="2009-07-28" version="1">MGQLLSHPLTEKTIIYNDYEHLSQQASRRRSSEHSSSANKHRAQKGAQFFNCVGSMQGYRLTQEDAHLVANCDSMMELNFYNPFTDREEMLSMSVFGVFDGHGGDECSKYVSSENDAYQGIRKWIRLSFETHEYGPVKGLESKSFKRRFRTIEGLVSQVLKDAFLLQDRELYCHYANSSCGSTGILAVIINEKKLYVANTGDSRCVLSTKGRGVKTLSYDHKPQHIGELVRINDDGGTVSLGRVGGVLALSRAFGDFQFKTSVSYTNSAHGPAAAQRYVAPAQESQVTVEPDVICHQISYDRDEFLVLACDGIWDLYSNRNLVQFIKYHLMLGQKLDDIVTKLLDHGINSADSNTGVGFDNMTIIIIALNKQGETLAHWYGKMKSRLEREKGLTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLI0</accession>
    <name>C5DLI0_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transcriptional activator HAP2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0G00880g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the NFYA/HAP2 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555078.1">
        <property type="nucleotide sequence ID" value="XM_002555032.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLI0"/>
    <dbReference type="STRING" id="559295.C5DLI0"/>
    <dbReference type="GeneID" id="8293336"/>
    <dbReference type="KEGG" id="lth:KLTH0G00880g"/>
    <dbReference type="eggNOG" id="KOG1561">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080763_1_0_1"/>
    <dbReference type="InParanoid" id="C5DLI0"/>
    <dbReference type="OrthoDB" id="1097733at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016602">
        <property type="term" value="C:CCAAT-binding factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018362">
        <property type="entry name" value="CCAAT-binding_factor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001289">
        <property type="entry name" value="NFYA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12632">
        <property type="entry name" value="TRANSCRIPTION FACTOR NF-Y ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02045">
        <property type="entry name" value="CBFB_NFYA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00616">
        <property type="entry name" value="CCAATSUBUNTB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00521">
        <property type="entry name" value="CBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00686">
        <property type="entry name" value="NFYA_HAP2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51152">
        <property type="entry name" value="NFYA_HAP2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="32"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="146"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="225"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="264"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="46"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="88"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="231"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR24641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="308" mass="34457" checksum="4D51486AFA498549" modified="2009-07-28" version="1">MTMDNARGNHSAGEKCDTAAIKASVILDMDATKEFPPLESDAQPCQRRDVNGKEELNPNIIGSSLGRQNVEVPYEDEQSPTNMYLYDQPQTNASRNESQTESHVDPTALNEEDLSTSVDHHASLEVQFPETEVSGVSIKPVEQPKYPVDSKIGARDGSQQQGGDGSIVSQAESEIEQVAGSTIGSLSEPTEQPFYVNAKQYYRILKRRYARAKLEENLKISRERKPYLHESRHKHAMRRPRGQGGRFLTAAEIAELKQHKELQLNLDIKQQDPADQSSKNVEEETTEEPKSQTAKRSSGKHMSEVVRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLL4</accession>
    <name>C5DLL4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0G01628p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0G01628g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555112.1">
        <property type="nucleotide sequence ID" value="XM_002555066.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLL4"/>
    <dbReference type="FunCoup" id="C5DLL4">
        <property type="interactions" value="33"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLL4"/>
    <dbReference type="GeneID" id="8293370"/>
    <dbReference type="KEGG" id="lth:KLTH0G01628g"/>
    <dbReference type="eggNOG" id="ENOG502RYK1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034017_0_0_1"/>
    <dbReference type="InParanoid" id="C5DLL4"/>
    <dbReference type="OMA" id="MYYPNYA"/>
    <dbReference type="OrthoDB" id="244495at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20557">
        <property type="entry name" value="CYCLIN_ScPCL1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013922">
        <property type="entry name" value="Cyclin_PHO80-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615:SF27">
        <property type="entry name" value="PHO85 CYCLIN CLG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="403" mass="44998" checksum="0E7C041984025784" modified="2009-07-28" version="1">MASYMYYPNYAAPQLQPFHGHSQSMGAPPVGNYGYYQAAQPMQPYYQPQPHRSFHNHSSSYPMVMPPPGMGYLAPPAPSHHMPLPPVQPQLQPPVQQHPPPAQNPESQVNGGVREVLDYDLKLMSEFVVKNAYLVFDNDDAINVESSSVVDVFTKGVSSVLNATRLPSVTIFMALDFLCKYISKLPAGVEAVGGDSVNVIYQNLMIALVLANKFNDDKTFTNKSWSHATGMELSVINQFERAWLAVFEWRLFDDNFVLFDEYSNSFKQYCQEKSSPVHQYNAPSLPPSASCVSGLSPVQQNCSGFQTPMQAPPMVYSSPCFTEERSTTSNSFYQPTFASPISNESPMTKQGMNGYSYNYYSAPQPAPALAPMSSNLLWNNRVDEFYPQSQFAPPNKAYYCYSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLS5</accession>
    <name>C5DLS5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0G03080p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0G03080g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555173.1">
        <property type="nucleotide sequence ID" value="XM_002555127.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLS5"/>
    <dbReference type="FunCoup" id="C5DLS5">
        <property type="interactions" value="1299"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLS5"/>
    <dbReference type="GeneID" id="8293436"/>
    <dbReference type="KEGG" id="lth:KLTH0G03080g"/>
    <dbReference type="eggNOG" id="KOG2081">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005996_0_0_1"/>
    <dbReference type="InParanoid" id="C5DLS5"/>
    <dbReference type="OMA" id="LECITSW"/>
    <dbReference type="OrthoDB" id="435593at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000266">
        <property type="entry name" value="mRNA transport regulator MTR10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013598">
        <property type="entry name" value="Exportin-1/Importin-b-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051345">
        <property type="entry name" value="Importin_beta-like_NTR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057941">
        <property type="entry name" value="TPR_TNPO3_IPO13_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057942">
        <property type="entry name" value="TPR_TNPO3_IPO13_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058537">
        <property type="entry name" value="TPR_TNPO3_IPO13_4th"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12363:SF53">
        <property type="entry name" value="MRNA TRANSPORT REGULATOR MTR10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12363">
        <property type="entry name" value="TRANSPORTIN 3 AND IMPORTIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24138">
        <property type="entry name" value="TPR_TNPO3_IPO13_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24140">
        <property type="entry name" value="TPR_TNPO3_IPO13_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24139">
        <property type="entry name" value="TPR_TNPO3_IPO13_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08389">
        <property type="entry name" value="Xpo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="1">
        <location>
            <begin position="27"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="966" mass="109212" checksum="3F8FB932A51E06B2" modified="2009-07-28" version="1">MSYNIGDIGTALQCITSSSEQETKKQALQFLEHFQKSPEAWNLCHEALASNGGQISLELQVFSAQTLRNKVTYDLSQLDGHLFTFKDSLLQLITQHSQKLIVTQLSVALARLAIQFLEWREPIAEIIGSLRQFPSKLLEFLKILPEETLDIKSTPLSEDEFRSRTHELIDQIAGDVLQYLISAAESLRSGSTEVSVSQLLNCVNSWAYEIPIEELLSVNTLMSLIFHALNQGEESDPEVFDVAVECMCTVLKETRDVPSEAVIKALYEQLVSMQTTLLPVDQISDFEDYAEVMDGLTRVFVEAGEAWCIHIAKNPQIFKPLVSVLLLLTCKNTDLDVVKYTFPFWFNLKQMLVLPRFKDQRAAYQDIFTELVDGIITHLHYPTGSFSSREEEDKFRDFRYDMGGVLKDCTAVVGSATALSRPYEKITNALNEPNPLANWQNLEAPLFSLRTMAQEISKNENVVLPQLFRLLCTLPEHPKVRYATTLVLGRYTEWTSKHPDFLEMELNYIFNGFQHANGSIEILTASSHALMYFCQDCSSLLSGFVSQLIEFYWKIETMVESESLFEVCQGLSCVIDRQTDDQVGASLELFLKPHLSKLVDAVSVWKANNNDKKATAEVCGKIDLIFAIFEELKPRYETPEQGREPLQPYICTIWETLESLLVNQEAFNNPDVAEIAMKWVRKVALNFHIFIAPILPSVANFLAQSYASTGFGVYLWCSGSIIAVFGDDESFPIDQQTKEAVWEFTCTQCVTFMNNFTGIEATKLESYHEAIQDFFMMMTDVVMFFPDRFVTSDILLSPVFSIGLNCVTKITNYDAYITIVRFLDDVLSWGFETPPISTATLDVVPEQWRQKILTEVVQAQGSLLVRTLLIGLVTNFSSDAHPDAIGCLVKCLRLCQDCSANDPTVSLGWLNEAMDTLGNVSAQERTNLVSTVGNALPQKDMRRVRTGLKDFVSWYTRKNVSPRLYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLW8</accession>
    <name>C5DLW8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0G04136p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0G04136g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555216.1">
        <property type="nucleotide sequence ID" value="XM_002555170.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLW8"/>
    <dbReference type="FunCoup" id="C5DLW8">
        <property type="interactions" value="153"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLW8"/>
    <dbReference type="GeneID" id="8293482"/>
    <dbReference type="KEGG" id="lth:KLTH0G04136g"/>
    <dbReference type="eggNOG" id="KOG1363">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_414514_0_0_1"/>
    <dbReference type="InParanoid" id="C5DLW8"/>
    <dbReference type="OMA" id="VYAFVEC"/>
    <dbReference type="OrthoDB" id="1026733at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14273">
        <property type="entry name" value="UBA_TAP-C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001012">
        <property type="entry name" value="UBX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050730">
        <property type="entry name" value="UBX_domain-protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23322:SF1">
        <property type="entry name" value="FAS-ASSOCIATED FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23322">
        <property type="entry name" value="FAS-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14555">
        <property type="entry name" value="UBA_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00789">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00166">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50033">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBX" evidence="2">
        <location>
            <begin position="430"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="366"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="371"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR24779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="586" mass="67763" checksum="C359E9634776BA6E" modified="2009-07-28" version="1">MPVIQHGDQEIHLSHDEEEKLNNFQVITTFPEESLPQIVKLLQDHGWQLEPALSRYFDGNWQENLNPPPVAARPHTPPIPQRPSNPVTPFMFGDTASLVPRLPLVKRLPLDFKEKFRYAGLDKRPEEFSTHPALFLLVLLPKVLFKIGTGIFTLLWSIISFGFRTEGVNTNHIHRVPESPSENVGSIHETLESIFNGNTELSSLVSSKPFNEVYDECEKEFKFLLVVCLGDVETDEAGARDANSRLFLESILNDPSTLQILREHSENLVIYMRLAQDMEMWSLTKQLKLRYTPECLLIGNVLNSKDSVNGATRMSVLSRLKVSTLRKFQNSLKIIVERHSAELIVSRSEQEELRMAREIKQMQDQAYQESLKQDQEKEQRRQAAEEEAKLKREAELIKEMEFKTTQTIQKLRILSFCLEHLESLTSSNITQEKHATLQIRTPNGRRFVKKFTGVSTLEYIYRVAKCFLFLDLESFNKDHVLQCMATKVKEMAQDEDVLCFKDERNIEDLRIDSQPEELSQILQNELPKWSTPDCEVELDIDFELVTPFPRFKLPNDKDVLIKDTSQIWPNGSILVEYLEDAEYESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMW7</accession>
    <name>C5DMW7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">KLTH0G12254p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0G12254g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555565.1">
        <property type="nucleotide sequence ID" value="XM_002555519.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMW7"/>
    <dbReference type="FunCoup" id="C5DMW7">
        <property type="interactions" value="628"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMW7"/>
    <dbReference type="GeneID" id="8293847"/>
    <dbReference type="KEGG" id="lth:KLTH0G12254g"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021006_0_0_1"/>
    <dbReference type="InParanoid" id="C5DMW7"/>
    <dbReference type="OMA" id="FQHTPTK"/>
    <dbReference type="OrthoDB" id="3437960at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000125">
        <property type="entry name" value="Putative zinc finger protein 143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001752">
        <property type="entry name" value="Transcriptional factor SWI5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050329">
        <property type="entry name" value="GLI_C2H2-zinc-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19818:SF144">
        <property type="entry name" value="METALLOTHIONEIN EXPRESSION ACTIVATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19818">
        <property type="entry name" value="ZINC FINGER PROTEIN ZIC AND GLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="583"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="613"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="250"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="350"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="518"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="180"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="356"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="368"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR25128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="746" mass="82458" checksum="952B26B7D8757E3B" modified="2009-07-28" version="1">MTSTREQWFNSADVLNLEVPEQGDLGYAADFSKAPSAGFNSNPGDQNAMDAELGNFFSSNYEDIDDFLTQELKDLDIPMVPQKFAAGTDSQAFLNYDAVWTRDEGTTGTPRKDHKRGPSGTAIFGFSNHNKQLSISKAGSETLLQGNPSSSQLLNYSAANTDKHYDAQNGNLNSLILKQQEELRLALERQQHMNEQLEEQLRHSQLQQKQLQMALQEQAIATHQLSGGISPAKTPSSRASHNTERNIIITSNSSNGGYKFPPPQTASSSKNGVISPISGTSVSGSPGRKAHKLRMPLVSLNGTHTDSSEEYSSPENFLGSLNGRINTNGVQKVNHCFKRSGDRGAPAALNFKDKFSSNNPITPQQQVRAGRGSSASSSPRINHHKAKESISSSASTIPQVLEDDDAQSSGAPDEGVIGLGIKFAGAKPKPKYTLNKPPPLDLLPTIPGSMDNTPMNSKLISTGDPSALPQKHVFQHTPTKNTFNNPHNSRTTLESAPNIFCTLKEELKPPDFYHSDDDFDEPESEFNHAKTPSPILKSQSRFEGSSPIIEHAHSSPVKITRKPTTLPPGEIDKYVKELPDKTFECLHPDCGKNFRRRYNIRSHIQTHLEDRPYICDFPDCNKAFVRNHDLIRHKKTHAEKCYICPCGKKFVREDALLVHRSRMICVGAKKFSNVVIKKSPRRRGRPRKDGTFSVNNSPVKESVNRDTNGTIALQMEEQLRKELIENGLLDPSYSMPDMNSPIEESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMW9</accession>
    <name>C5DMW9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TBP-associated factor 12</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Transcription initiation factor TFIID subunit 12</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0G12298g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF12 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555567.1">
        <property type="nucleotide sequence ID" value="XM_002555521.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMW9"/>
    <dbReference type="FunCoup" id="C5DMW9">
        <property type="interactions" value="606"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMW9"/>
    <dbReference type="GeneID" id="8293849"/>
    <dbReference type="KEGG" id="lth:KLTH0G12298g"/>
    <dbReference type="eggNOG" id="KOG1142">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021602_0_0_1"/>
    <dbReference type="InParanoid" id="C5DMW9"/>
    <dbReference type="OMA" id="ADDFITN"/>
    <dbReference type="OrthoDB" id="2193432at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07981">
        <property type="entry name" value="HFD_TAF12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000011">
        <property type="entry name" value="Transcription initiation factor TFIID subunit 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037794">
        <property type="entry name" value="TAF12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003228">
        <property type="entry name" value="TFIID_TAF12_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12264">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12264:SF21">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03847">
        <property type="entry name" value="TFIID_20kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription initiation factor TFIID subunit 12" evidence="9">
        <location>
            <begin position="367"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="75"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="222"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="222"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="245"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00075089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00093657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR25130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="500" mass="54356" checksum="5FE8721A4855D055" modified="2009-07-28" version="1">MSSNPNSGQPAQSGNNQQGTPAQRLTPESLQQMAYKFSQCFNEAKRVGLQTPQGQELLKQASRIKAMYDAYRQRQQSAQGFEQGGATSNTGDNTAGTPTNARSPNGTPNNNMSSNIQNLLTPQQNEAYGRLIQTSNENGEKIKGEYTYLKKQIEILDGEIKKRQGDPATVKQLETNKTEILNKLRGFSASYLALNQKLREDKKKFYIECAATNPNLKRFLQASTQQQRANSTASQNASPLPAATGANQASSPIQQTGAPSQVQQQTATPSQPRSGVNSRSPTQVTSVNAAAHLANNATSAARQAIFKQPNPSVPVSETITQRMPTPVVHRSNRPTITGGSAMNAAALTTPVMTKLPPYEVDNERVMSKRKLRELVKSVGIDEGDGETTIDGDVEELLLDLADDFITNVTSFACRLAKHRKSDNLDVRDIQLHLERNWNIRIPGYAADEVRSTRKWNPTTSYNQKLQGINSAKVTKTTSAAQAAAAQAVSSNADSKNQPAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DN09</accession>
    <name>C5DN09_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0G13244p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0G13244g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555607.1">
        <property type="nucleotide sequence ID" value="XM_002555561.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DN09"/>
    <dbReference type="FunCoup" id="C5DN09">
        <property type="interactions" value="147"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DN09"/>
    <dbReference type="GeneID" id="8293890"/>
    <dbReference type="KEGG" id="lth:KLTH0G13244g"/>
    <dbReference type="eggNOG" id="KOG2997">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017706_1_0_1"/>
    <dbReference type="InParanoid" id="C5DN09"/>
    <dbReference type="OMA" id="RWNRLDF"/>
    <dbReference type="OrthoDB" id="2117972at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09917">
        <property type="entry name" value="F-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045464">
        <property type="entry name" value="Hrt3/FBXO9_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12874:SF9">
        <property type="entry name" value="F-BOX ONLY PROTEIN 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12874">
        <property type="entry name" value="F-BOX ONLY PROTEIN 48-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19270">
        <property type="entry name" value="FBO_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="F-box protein Hrt3/FBXO9 C-terminal" evidence="3">
        <location>
            <begin position="197"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="80"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="80"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="90"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR25170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="369" mass="42992" checksum="58D2FDEE15807687" modified="2009-07-28" version="1">MTGIHHDTHHESHLLGDSGNVGLEAVNLWKRGVLKEKDGLMMDAIGCYRRALKMDGEVEKQYRKLVHQETQTMREMEKLSLQSRLPSTQEGKEAEKAGDLVKESGEQVEQNDLPCWILEMLPDDILKKIVLETVLISGESWLNLSLTCRRFHRLCFREPEPYRTFAQHIYSLQHYDKAAMALNGLSSIKTLEEAFWGVDYRRMLEERAYVKFQGCYISVVNYLRQGASAEGSSSWINPVHMITYYRYFRFYPDGSCLRLVTTDEPSNVVRGYDRDKDIKNADLCHWSLSIDDDLSLLTITRRNDKYSFEEKLRIHKHAHKTHQRLKWVSSGAFDNRGERIDFSMRNEKPFSFSRVTSYKPVKEDHGGNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5DN37</accession>
    <name>C5DN37_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0G13860p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0G13860g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555635.1">
        <property type="nucleotide sequence ID" value="XM_002555589.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DN37"/>
    <dbReference type="FunCoup" id="C5DN37">
        <property type="interactions" value="45"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DN37"/>
    <dbReference type="GeneID" id="8293918"/>
    <dbReference type="KEGG" id="lth:KLTH0G13860g"/>
    <dbReference type="eggNOG" id="KOG1938">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015504_0_0_1"/>
    <dbReference type="InParanoid" id="C5DN37"/>
    <dbReference type="OMA" id="KLADWSM"/>
    <dbReference type="OrthoDB" id="203724at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990072">
        <property type="term" value="C:TRAPPIII protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024420">
        <property type="entry name" value="TRAPP_III_complex_Trs85"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12975:SF6">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12975">
        <property type="entry name" value="TRANSPORT PROTEIN TRAPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12739">
        <property type="entry name" value="TRAPPC-Trs85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="477"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="589"/>
            <end position="620"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR25198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="708" mass="80421" checksum="84584327120747BB" modified="2009-07-28" version="1">MPLSFGKYMNLAFHLDSALEPVPTEISRKIVSSAVSPVVTVTSTAALDEHIQESYGVDSLYMLLRYFGDCVSDRDQAEAGPELGTESGKNRNRARSNSLFQRQASHYLRFTRPLTDLIDVRESRDLLFDYHSLEVFLEKYLSLVEANTTSDTPHTLLQHSIYHKFFTTAISSTAHLSPYESFNHPVASLLALDISKDQGYEEARDLLIAFKNMHNTTPHFPAFINVNDILPVFLLCYEEDSREQFELCQSLSKTIKKQLFVESLLLPCWGQNINAGASPSRILHQPIMSSLEETMYSMMKESTITLPLSLINYIYDRLTNLIDELMIPFMKRKISFWDESILQPRKSIFPNSKFFRKLISKTPAPPPASDSATHSQQGVAYFAATSNEFILRKLADWSFMLSDFKNAYSTYELLSKDFEQRPEYLASCLEWCALSVLMGAQSIVTVKMIKNDIDPLINRALKSYELSARKAKEKNAQLKSSALAKKAEKDSKSATLPSTVSDEHSGQELDEGVSSAPSQSAQSYETRCMLLAAELFLSLSDTWTATPYAIKYLETILDECTLGSLSEIVLWERLAYCYALRVDPRVKGKLPSLDGQSGQNEKRGEESEGSDESDDETQKYDHKISQNCMASLGLARRRKSTLFRLIATKKWTESKQWRQAYWGLKDIEVVYSELDFANRDDLILEKLRKKIHEHEPLPQKLNTSFIST</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNI6</accession>
    <name>C5DNI6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0G17358p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0G17358g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555784.1">
        <property type="nucleotide sequence ID" value="XM_002555738.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNI6"/>
    <dbReference type="GeneID" id="8294077"/>
    <dbReference type="KEGG" id="lth:KLTH0G17358g"/>
    <dbReference type="eggNOG" id="ENOG502RNFM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_274068_0_0_1"/>
    <dbReference type="InParanoid" id="C5DNI6"/>
    <dbReference type="OMA" id="LATEWCP"/>
    <dbReference type="OrthoDB" id="4033386at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050863">
        <property type="entry name" value="CenT-Element_Derived"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004875">
        <property type="entry name" value="DDE_SF_endonuclease_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19303:SF74">
        <property type="entry name" value="POGO TRANSPOSABLE ELEMENT WITH KRAB DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19303">
        <property type="entry name" value="TRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03184">
        <property type="entry name" value="DDE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE-1" evidence="3">
        <location>
            <begin position="442"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1137"/>
            <end position="1168"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1104"/>
            <end position="1131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1154"/>
            <end position="1165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR25347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1178" mass="132652" checksum="3B3B359311657527" modified="2009-07-28" version="1">MASIGRVLYQEDYRKYKIVKRELLVFLKEHRLDASSPSLFLLLSHNTHSNGTNLYLPLMVAERSAAQNASTNYRVIHHFKHHQNFPAVIQDANGNSNVSTSISICSTCPWDLVVPLLQYLHASARPSGDYSHLTCLQKQKKFNGEKARVAAQEYLNDPKSSKSIVANRYSISLSTLNDAVKRLKRQREVSEGHNQLLDYKSAPDPLSRKRVASVALLNVLNSSLSPSKKSRLVPHISFKYNIPERTLRDRLKSFNPSVSKAAEGVRRRKLSPSAEQFLTTFYAICNVQNSQITVQQADAICELMAATTDCPEIKFFDHDLEENGAINLPRLLREQETRLGRNFGWKFRQRDGFHSSKARQIDCSRLKASDVNNIAMWLSEDAYKILSTTPAELVFNADEIGFGHTINSTNRHSRYVSFTNSQKMHGKGVVYKAKEAATNTKTLTTVTACVSAAGEIVPPMVTASSSIAKLKTECHTFNPRFETLARSKLHPGVGENTLLLFTSNGWNNSKAFFEYIKWFDSHTEHLAFDQGDNMPQKQRVLIIDNHYSHYCRPVLDYVATRKITLLLLPPNTTHVLQPLDVGVFSCLKKKVRTLATEWCPHPSAGADGKPRYGHFDILCNFMNALNHTLSFEFEQTKISQAFATAGIYPPEKSLFNPAIRTVMKHFDAHARMKDIFLNYILPTTQCSRPESRTITGATFTQTENPSSPVVPLIGSEAFFIGEEDLESLDYLAVNAQEVMLGTAGGQETIEESEYDVSSSQASDKIELACALDDKFISKFTFEDVLKMISDLIGGEVDTESISLVPKLMERLDKCSELAFGSGSHLTITKELFLEQQANVMGIIMYLIYFGACFLGVREQNKIEEALQCFDNKETSVRATGASVEKQTTSVLRDLSKKQFFKLFPKIAGSIDRDLSKKISLIQNTTQDSESMSDELINELGFSVWEFLQKMIKNENSSLSLQPEQLRNQLLSKAFNAPEALAKAATVRSLFDHNGSMPFSKKDLPLVSKIESISAIPEELLDPSVSDEEFARRVSELREIAAEAMRDMTYWDDEEEDSADSMLGSTQTEDSALDTEERNKKHLQNWFLVYHVDNVVGRIMKDRENAKLREENRMHQKKIQALTNAIAEMANDVRYSETPETLLETPGLRLGTPDLRSETPDPRLETPDPFLETELVESF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNK5</accession>
    <name>C5DNK5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0G17798p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0G17798g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555803.1">
        <property type="nucleotide sequence ID" value="XM_002555757.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNK5"/>
    <dbReference type="FunCoup" id="C5DNK5">
        <property type="interactions" value="80"/>
    </dbReference>
    <dbReference type="GeneID" id="8294097"/>
    <dbReference type="KEGG" id="lth:KLTH0G17798g"/>
    <dbReference type="HOGENOM" id="CLU_390819_0_0_1"/>
    <dbReference type="InParanoid" id="C5DNK5"/>
    <dbReference type="OMA" id="ARPETIC"/>
    <dbReference type="OrthoDB" id="4034825at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="594"/>
            <end position="617"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR25366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="706" mass="80254" checksum="8474F49B53370FDA" modified="2009-07-28" version="1">MSEMYKDFIQPTLGYLNSLRHSKSTGPSILSAPSHESDVGQSDSDQSFLCDRGTGPKLHRASPTSTPGDLFDPYRLDCEQAPFKKGSADCDTIGDISDGGSVIRYSLNSSMTHSYAHDNSRKMESLTATATNENNELTLKLLNAYLSLYVADARYAQDNGCAEYSVEANQISSPQEIIGTIPESIGPLTDIIGFVDLTSQTLFKFVTNSGFLQNCNANSWDQTELCELKNHISAILECLILEYAPQEQQPLFEKFEKSLALLKRTRFDTRCWKQTRSLQKLHGDDWILEKLETLKFVPRTLLDALNFDVIMPQDASPFSKSRKGDIETLEVFTTLYAILLLEIKKLFELVVLFAQTAGIIKDLECPLLLGCLEGFLRNSVVSGNTELVVGLDKLVQNWTHSCESFKTKTVHWANHFLSQWKVARPETICETTFYSRRSLEQASQNPKSYEFDLVHLFINEVMDICALDSLNTEVLEATTVKEKASASGLGWLTIFQDEDPHANPSTIQTRTIETSASNDFFRYQNNYSLIEDETTIIIGGQGHFWTRGRRNHEHRHTQQLRNWIRRGFHSNDVGAKHQILNKCQRAKNKLKDLAQKLSKSEGPQSQLRNNKVAPGKEPTQVLKSEWHRNAGTVRVAAKGAVIDNKNNNQKSVTEERNHNSYYHIAAFHGAFEATTYSDHYEHRPRRYENLRRKKDRFMFLFFGEER</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNN6</accession>
    <name>C5DNN6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0G18590p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0G18590g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the oligoribonuclease family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555834.1">
        <property type="nucleotide sequence ID" value="XM_002555788.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNN6"/>
    <dbReference type="FunCoup" id="C5DNN6">
        <property type="interactions" value="803"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNN6"/>
    <dbReference type="GeneID" id="8294129"/>
    <dbReference type="KEGG" id="lth:KLTH0G18590g"/>
    <dbReference type="eggNOG" id="KOG3242">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064761_0_0_1"/>
    <dbReference type="InParanoid" id="C5DNN6"/>
    <dbReference type="OrthoDB" id="270189at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06135">
        <property type="entry name" value="Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000003">
        <property type="entry name" value="Oligoribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022894">
        <property type="entry name" value="Oligoribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Ribonucl_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003765">
        <property type="entry name" value="PRK05359.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11046">
        <property type="entry name" value="OLIGORIBONUCLEASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11046:SF0">
        <property type="entry name" value="OLIGORIBONUCLEASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exonuclease" evidence="6">
        <location>
            <begin position="75"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="279"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="309"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR25397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="323" mass="36303" checksum="FA645FC342D01FDB" modified="2009-07-28" version="1">MWTSIAKKRLPRSILTASTSKRLLQNTKKTALSNIYKQKNPYLTHKASAPKFVKLIRMSGSELNTKQEAPSISRPIVWVDCEMTGLDHVNDHIIEICCIITDGDLNIIDEAGYESVIHCDKKIMDAMNEWCIDHHGSSGLTQKVLDSTKTTGQVEEELLAYLRKYIPAERKGVLAGNSVHMDRVFMMREFPKVIDHLFYRIIDVSTIMEVSFRHNPDLASVFPRKKGAHTARADILESIAQLKWYQDHYLKNSSETQEFVEGRRREMVIEEVAQSVASATTTVGKTAEEAAGNPPTNVTDTAAAAAKRSSTDTELPLKKQKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNT4</accession>
    <name>C5DNT4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0G19668p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0G19668g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555882.1">
        <property type="nucleotide sequence ID" value="XM_002555836.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNT4"/>
    <dbReference type="FunCoup" id="C5DNT4">
        <property type="interactions" value="211"/>
    </dbReference>
    <dbReference type="GeneID" id="8294178"/>
    <dbReference type="KEGG" id="lth:KLTH0G19668g"/>
    <dbReference type="eggNOG" id="KOG2533">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_0_1_1"/>
    <dbReference type="InParanoid" id="C5DNT4"/>
    <dbReference type="OMA" id="HEKDICT"/>
    <dbReference type="OrthoDB" id="2985014at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17327">
        <property type="entry name" value="MFS_FEN2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000013">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000018">
        <property type="entry name" value="MFS transporter permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF24">
        <property type="entry name" value="NICOTINIC ACID PLASMA MEMBRANE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="160"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="195"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="230"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="303"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="336"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="364"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="390"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="423"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="451"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="69"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR25445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="508" mass="56186" checksum="983C58EBAF85AFC6" modified="2009-07-28" version="1">MNGHSSPAKELEVTTINECCLAKSSTQLSVVEGEVCDQSISGDTPTTHGTLQIDPAEEKALVRKLDRRFVPVLAFAYFLQSLDKTNIGNAYTSGMKEDLDLTPKQYSNAVSVLYSTFLIAQVPTTLYLRVIPPRYFMSGMVFCWSIITLCSGFVKSYHSLIALRVLLGAFEGGYFPAMVLLVSMIYKPEEQAKRIAFFFSCAALAGAFGGLIATGLATVHGAGGLNGWRWLYIIEGLVSTTAAVWIYFGLPTDVEMPAFMNNREKSLLKVRSKQRLQYIGPQPNFSWDFVWDALKDFKVYSGLAIQFCQNIILYAFTTFLPAILKLGMGYSSRQAQYLTVPVYILAAAVFLSSAYFSDRFNMRGPIILCFNIVTIIGYIIMLTAKTSGVKYFACYLMTFSAFTGPGLNVTWVSNNIAPHYKRATAIGLSQMFGNLAGAIAGQVYIKPPYVLGNSFSLGCVGFSSLLVALQIILFYRINERRAAILRGAVVDKFKKRSGDKDLNFKYCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>C5E1X8</accession>
    <name>C5E1X8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0H00506p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0H00506g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555901.1">
        <property type="nucleotide sequence ID" value="XM_002555855.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E1X8"/>
    <dbReference type="STRING" id="559295.C5E1X8"/>
    <dbReference type="GeneID" id="8294213"/>
    <dbReference type="KEGG" id="lth:KLTH0H00506g"/>
    <dbReference type="eggNOG" id="ENOG502RZYI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_758796_0_0_1"/>
    <dbReference type="InParanoid" id="C5E1X8"/>
    <dbReference type="OrthoDB" id="4035904at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE Tnp4" evidence="3">
        <location>
            <begin position="244"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR30039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="365" mass="41228" checksum="66694DFD67F522E4" modified="2009-07-28" version="1">MMKTSDVERLAYLHQERHLKNDGFCEFIENVATNQYLLRYTYGNLVIRQATNKLTQRTPQLVLETLEDNGVFHNEERILIEKFTEQLIANQIQFETRKRSRGRGKNFCYNKNYHSGGKFKLKYTNTDFRKLTGFCRSDFDRVVEALKMPSYIGAYSLDAFECFFIFVVRLKSGCTYTVLTSLCGREASTISRIITFVTCYLFHIASSCLSVTNASWLAGRLANIDQSLLSSSRSISNSRIVGFIDGTHVKIARPHGNARLENAAYSGHKKTHTLKYQAVTTPDGICIDLAGPFAGSQHDSFLFANSGLYRRLKPALKVGGIQYRISGDPAYRSSDLLTVGYKGQNLTNGQKEYNKILSGSRVSIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>C5E217</accession>
    <name>C5E217_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0H01430p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0H01430g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the epsin family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555940.1">
        <property type="nucleotide sequence ID" value="XM_002555894.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E217"/>
    <dbReference type="FunCoup" id="C5E217">
        <property type="interactions" value="165"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E217"/>
    <dbReference type="GeneID" id="8294253"/>
    <dbReference type="KEGG" id="lth:KLTH0H01430g"/>
    <dbReference type="eggNOG" id="KOG2056">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012678_0_1_1"/>
    <dbReference type="InParanoid" id="C5E217"/>
    <dbReference type="OMA" id="STEFYDI"/>
    <dbReference type="OrthoDB" id="4033880at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030125">
        <property type="term" value="C:clathrin vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16991">
        <property type="entry name" value="ENTH_Ent1_Ent2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000006">
        <property type="entry name" value="Clathrin interactor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276:SF110">
        <property type="entry name" value="EPSIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276">
        <property type="entry name" value="EPSIN/ENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01417">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="9">
        <location>
            <begin position="11"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="143"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="327"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="483"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="187"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="164"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="328"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="355"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR30078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="502" mass="57759" checksum="13A2EA78DA24A069" modified="2009-07-28" version="1">MSKSLMRRAKNVVNGYSTTQVLLREATSNDPDGPSLDLMDEIAERSWDSVSFFEIMDMLDKRLNDKGKNWRHVAKSLTVLDYLVRCGSEHCVPWAKENLYIIKTLREFTHEDETGVDQGQIVRVKARELTALLQDEERLREERELKGRNRRPRRRKNSDSYDDDMQRALEESRMTAEQEEAERRRKLEMYDNSADDLQTALQLSKEEEELRRLRELQMQQQMQQAQQQMFGLQQQQTQQPMYYDVFGNPISAEEYLQYQQQMLEQQQMLEQQQQQQALAQQLAQQQYLAQQQYLAQQQQQQQALAQQQYMQAYAQQQNAQQQLLPLETGSNNPFAKNAQQPSAEDPSKYAAASSPQRTQQLSPQLQPQPEQFQQQQQQIPQQPLKQTRTGNQSVTDKFSDLNNLLASGTGLDTFGNTGQTRIPAQHTKTGTFINSQGTGYKQVSNMPQGKHNPFLGQQYTGLPSTSVVPSYTGYGFGNQNVQKQTNQNRANGADQGVSLIDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>C5E275</accession>
    <name>C5E275_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="11">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0H02706g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="11"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555998.1">
        <property type="nucleotide sequence ID" value="XM_002555952.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5E275">
        <property type="interactions" value="493"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E275"/>
    <dbReference type="GeneID" id="8294311"/>
    <dbReference type="KEGG" id="lth:KLTH0H02706g"/>
    <dbReference type="eggNOG" id="KOG0351">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001103_22_1_1"/>
    <dbReference type="InParanoid" id="C5E275"/>
    <dbReference type="OMA" id="LCHKNCD"/>
    <dbReference type="OrthoDB" id="10261556at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031422">
        <property type="term" value="C:RecQ family helicase-topoisomerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000729">
        <property type="term" value="P:DNA double-strand break processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031573">
        <property type="term" value="P:mitotic intra-S DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006312">
        <property type="term" value="P:mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17920">
        <property type="entry name" value="DEXHc_RecQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18794">
        <property type="entry name" value="SF2_C_RecQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000668">
        <property type="entry name" value="ATP-dependent DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000296">
        <property type="entry name" value="ATP-dependent DNA helicase RecQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000340">
        <property type="entry name" value="Bloom syndrome, RecQ helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.80">
        <property type="entry name" value="HRDC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004589">
        <property type="entry name" value="DNA_helicase_ATP-dep_RecQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022758">
        <property type="entry name" value="Helicase_Sgs1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010997">
        <property type="entry name" value="HRDC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044876">
        <property type="entry name" value="HRDC_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032284">
        <property type="entry name" value="RecQ_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018982">
        <property type="entry name" value="RQC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00614">
        <property type="entry name" value="recQ_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF153">
        <property type="entry name" value="RECQ-LIKE DNA HELICASE BLM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11408">
        <property type="entry name" value="Helicase_Sgs1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16124">
        <property type="entry name" value="RecQ_Zn_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09382">
        <property type="entry name" value="RQC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00956">
        <property type="entry name" value="RQC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47819">
        <property type="entry name" value="HRDC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="645"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="14">
        <location>
            <begin position="829"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="90"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="314"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="418"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1157"/>
            <end position="1196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1322"/>
            <end position="1403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1182"/>
            <end position="1196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1326"/>
            <end position="1335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1347"/>
            <end position="1361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="1386"/>
            <end position="1403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR30136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1403" mass="155898" checksum="905257E15F043ECF" modified="2009-07-28" version="1">MQDNLRAQINWLKKEKAYQPKNEFLLRLLSSDVGGKKQKSQNGSEAQSQPEMSVIDLTADSRTIKASSDLGSSARVWNASAVFSTIEGNSTQRPGISYSNPAVTSRFGRTSESTQHSQKPASLPATSYVESGSLSANHISDQSSDAVRRTPNRQASLAELVPLQDRVIELLKKQTGLLMHKCSIIESTSLSEDAKRVKIQSEVNPSLSTLESEIRTAGSLVEAMKSEASHPVARTVGNLATTITHTPAISTVSIRTVANNSILRPPPRPQAPVVGVPQDLITVLDDDDDDDDDDVDVDGEEEPRVIAKVHTSAIGTEDRSSNPAKRSLRPHKEINYRIPELEDSLNYNMKPALAQSRQEDNAEEDTTMEAELEDSQFLLTAEEEHDEIHTSDHDFVADGLENLLDETDDGEYHEAVSNADGINSSQGGTFPEGPTQGSTFAGELSNIEVIGSSPVRQISEHQEYSRPKTPELDLVGTGYADDPEFDPIVDSIQQTFPNSYAHRSPVTGSITDNNIGRNQALQNSIDDTFTHSDSDDFEDFDAERENLTQKSGIRKLDDDLKIISEQKLDVSNARPSNIKQEVINVESLLDEGVENFQGGALSLSSIGAAGHGNTGPQFEWTAEVYHRLHNVFKLPGFRPNQLEAINASLEGKDVFVLMPTGGGKSLCYQLPAVVRSGKTSGTTVVISPLISLMQDQVEHLLAKNIKACMFSSKGTADQKRQTFNLFINGLLDLIYISPEMISASEQCKKAIGKLYRDKKLARIVVDEAHCVSNWGHDFRPDYKELKYFKREYPDIPMIALTATASEQVRLDIVHNLQLNNPVFLKQSFNRTNLYYEVLKKSKNVVFDICNAVKTRFKNQTGIIYCHSKNSCEQTASLLQRNGINSAYYHAGMEPDERLEVQQAWQANRIRIICATVAFGMGIDKPDVRFVYHLTIPRTLEGYYQETGRAGRDGKFSFCIMYYTFRDVRTMQTMIQKDKNLDRENKEKHLTKLQQVMQYCENLADCRRQLVLSYFNENFDSALCRRNCDNCKNSINTTTEERDVTHEALNIVKLVSSVQGDKVTLIHCQDIYKGSRNSKIVQAGHDQLEYHGMGKALPKSDIERVFFRLVTTQILQEYTVMNGRGFASNYLKVGPKATQLLNGNLKIVMQFAAAGESGSRQSSVPPAPATRAASTEFRKGHTNARQTTSSGSLARKTSSLIPDLGGFVYDDNGAGASSAPKKVSRPIVLGDKTNLLSHQEQLSVTSAYQELKERAISTARELRFNGFMSVISETALRKAAVVLPSTEQQFRQLPGLTSTQAQHYHFFKDLFVRLNVLRKSLPKDSSQRLSQTQTTSPFFHGPGASENNEERERDEEILRQIRDSQAPSRTSAVGKAPGTKPAGSQSKARRGRKSKWHSKSWSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>C5E277</accession>
    <name>C5E277_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glycine cleavage system P protein</fullName>
            <ecNumber evidence="7">1.4.4.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0H02750g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">The glycine cleavage system catalyzes the degradation of glycine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 7">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[glycine-cleavage complex H protein] + glycine + H(+) = N(6)-[(R)-S(8)-aminomethyldihydrolipoyl]-L-lysyl-[glycine-cleavage complex H protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:24304"/>
            <dbReference type="Rhea" id="RHEA-COMP:10494"/>
            <dbReference type="Rhea" id="RHEA-COMP:10495"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83143"/>
            <dbReference type="EC" id="1.4.4.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 6 7">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="7">The glycine cleavage system is composed of four proteins: P, T, L and H.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the GcvP family.</text>
    </comment>
    <dbReference type="EC" id="1.4.4.2" evidence="7"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556000.1">
        <property type="nucleotide sequence ID" value="XM_002555954.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E277"/>
    <dbReference type="FunCoup" id="C5E277">
        <property type="interactions" value="763"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E277"/>
    <dbReference type="GeneID" id="8294313"/>
    <dbReference type="KEGG" id="lth:KLTH0H02750g"/>
    <dbReference type="eggNOG" id="KOG2040">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004620_3_1_1"/>
    <dbReference type="InParanoid" id="C5E277"/>
    <dbReference type="OMA" id="RNLICTC"/>
    <dbReference type="OrthoDB" id="6537869at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005960">
        <property type="term" value="C:glycine cleavage complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016594">
        <property type="term" value="F:glycine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004375">
        <property type="term" value="F:glycine dehydrogenase (decarboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019464">
        <property type="term" value="P:glycine decarboxylation via glycine cleavage system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00613">
        <property type="entry name" value="GDC-P"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000005">
        <property type="entry name" value="Glycine dehydrogenase (decarboxylating), mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000007">
        <property type="entry name" value="glycine dehydrogenase (Decarboxylating), mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003437">
        <property type="entry name" value="GcvP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049316">
        <property type="entry name" value="GDC-P_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049315">
        <property type="entry name" value="GDC-P_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020581">
        <property type="entry name" value="GDC_P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00461">
        <property type="entry name" value="gcvP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003346">
        <property type="entry name" value="PRK04366.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11773:SF1">
        <property type="entry name" value="GLYCINE DEHYDROGENASE (DECARBOXYLATING), MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11773">
        <property type="entry name" value="GLYCINE DEHYDROGENASE, DECARBOXYLATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21478">
        <property type="entry name" value="GcvP2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02347">
        <property type="entry name" value="GDC-P"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3 6 7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Glycine cleavage system P-protein N-terminal" evidence="8">
        <location>
            <begin position="56"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="Glycine cleavage system P-protein N-terminal" evidence="8">
        <location>
            <begin position="524"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="domain" description="Glycine dehydrogenase C-terminal" evidence="9">
        <location>
            <begin position="832"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="6">
        <location>
            <position position="755"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR603437-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF21478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR30138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1019" mass="111134" checksum="2EF0A7DC3C2A3F91" modified="2009-07-28" version="1">MLRSRLLTSTKFIARSLHSGRPSLYAASVANVSSNQYAKIYKPAARSLEPLDTFQRRHLGPNPDNVQDMLKTMGYEDLDKFIETLVPPQVLERRPLQLEAPQKGFSEQEMLQHLQEIANKNKFQARNFIGKGYYGTVLPPVIQRNLLECPEWYTSYTPYQPEISQGRLESLLNFQTVVSDLTGLPVANASLLDEGTAAGEAMLLSFNTAKRKKSKFIVDKRLHPQTKSVLKTRATPFNIELVEVDPLEVEANKTILADKAVFGGLIQYPATDGTIIPGEIVKSIAESLHANKALLSVASDLMALTLLKPPSEFGADIALGSSQRFGVPFGYGGPHAAFFAVTEKLNRKIPGRIVGVSKDRLGSPALRLALQTREQHIKRDKATSNICTAQALLANIAANYCVYHGPQGLKDIAGRIYGFTTVLANKISAETPHTVVNNTWFDTLTIKLAEGTSSERLLKAAYEKYSINLFGVDENTVSVSLDETVTRKDLLNLLQVFGASEELPELLPEFPEELSRTDSILSNEVFNQHHSETAMLRYLHRLQSRDLSLANSMIPLGSCTMKLNSTVEMMPVTWPQFANMHPFQPVEQAEGYLELIKSLEADLADITGFDNVSLQPNSGASGEYAGLRVIKSYLEAQGQSHRNVCLIPISAHGTNPASAAMCGMKVVPVNCLSDGALDLQDLEAKAEKHKDDLAAMMITYPSTYGLFGPGIRAAIDAVHKHGGQVYLDGANMNAQVGLTSPGDLGADVCHLNLHKTFAIPHGGGGPGVGPICVKSHLGPFLPGHDLVNIATGAPSDLRISAVASAPYGSASILPISYAYIKMMGSTGLPFSSVVAILNANYMMSRLKPHYSILFAGDNSTTKHCGHEFIVDLREFKAAGVEAIDVAKRLQDYGFHAPTLAFPVPGTLMVEPTESENLQELDRFVDALIAIRREIDLYAAGDPKGAVLKNAPHSLTDLVCSTDWDARGYSREFAAFPLPFLKHNKFWPPVARLDDTYGDLNLICTCPSVEDVANDNAEHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2C0</accession>
    <name>C5E2C0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">KLTH0H03718p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0H03718g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the TRAFAC class translation factor GTPase superfamily. Bms1-like GTPase family. BMS1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556043.1">
        <property type="nucleotide sequence ID" value="XM_002555997.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2C0"/>
    <dbReference type="FunCoup" id="C5E2C0">
        <property type="interactions" value="1589"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2C0"/>
    <dbReference type="GeneID" id="8294356"/>
    <dbReference type="KEGG" id="lth:KLTH0H03718g"/>
    <dbReference type="eggNOG" id="KOG1951">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002486_0_0_1"/>
    <dbReference type="InParanoid" id="C5E2C0"/>
    <dbReference type="OMA" id="KLHVPMV"/>
    <dbReference type="OrthoDB" id="10260897at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000479">
        <property type="term" value="P:endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01882">
        <property type="entry name" value="BMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000105">
        <property type="entry name" value="BMS1 ribosome biogenesis factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012948">
        <property type="entry name" value="AARP2CN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039761">
        <property type="entry name" value="Bms1/Tsr1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037875">
        <property type="entry name" value="Bms1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007034">
        <property type="entry name" value="BMS1_TSR1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030387">
        <property type="entry name" value="G_Bms1/Tsr1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12858">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12858:SF2">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN BMS1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08142">
        <property type="entry name" value="AARP2CN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04950">
        <property type="entry name" value="RIBIOP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00785">
        <property type="entry name" value="AARP2CN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01362">
        <property type="entry name" value="DUF663"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51714">
        <property type="entry name" value="G_BMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Bms1-type G" evidence="12">
        <location>
            <begin position="68"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="323"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="371"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="423"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="563"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="623"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1128"/>
            <end position="1168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="7"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="427"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="461"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="481"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="563"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="579"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="636"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="686"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1128"/>
            <end position="1155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00061391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR30181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1168" mass="133111" checksum="9CBA8152CFDC5A0C" modified="2009-07-28" version="1">MDQSNKEHRKKSEKATAKKKLHTQGHNAKAFAVAAPGRMARNMMRSSDVNERKLHVPMVDRTPEDDPPPVIVTVVGPPGTGKTTLIKSLVRRMTKSTLSEVNGPITVVSGKRRRLTFIECPADDLNSMIDIAKVADLVLLMIDGNFGFEMETMEFLNLAQHHGMPRVLGVATHLDLFKSQSTLRATKKRLKHRFWTEVYQGAKLFYLSGVINGRYPDREILNLSRFISVMKFRPLKWRNEHPYLLADRITDITHPELLETQGKQIDRKVAIYGYLHGTPLPSTAGARVHLAGVGDYSISHVEKLPDPCPTPYFQQKLDEYERERVKEQGAGTTGAPRRRKRLDDRQKLIYAPMSDVGGVLMDKDAVFIDVGGKKPGDGPSFVPGQEKGEGERLVTSLQSVEKSISENFDGVGLQLFSNGQELHEADSDGENSVSEDEGDSNKDGGRKSLRKPKIYGKSIQEEDEEIDNLESDEEMEAGASDDDRPRQRRLVEVDFDAGNDASVNAHTDSEFELSDHEEELWNRSTASKLQGSKIRPRKWDIGKLIYMENIEPFDCIKRWKGELEDENEESGEQSEEEDFFKQADKPENKNTEAEDDLEKFKPHFETFKKLAAKWSSFDSLKGRFAGRPNLEQSSADNDEDDEELYGDFEDLENGDQEAASEQDSHSSNEDGDDGSDGAGSDNSFADFEKEEKKDLTTDQERERNAAKKESLRLQFEMEEGDNFKEDDPENEFDTWYELQKAKMAKQQEINNAELESMTPEQRQKIEGFKAGSYVRIVFDALPMEFVENFDARYPIIMGGLLPTELKFGILKARMRRHRWHKKILKTNDPLVLSLGWRRFQTLPIYTTTDSRTRNRMLKYTPEHSYCFASFYGPLCSPNTTFCGVQVVANSDTTGNFRISATGIIEEIDASVEIVKKLKLVGYPYKIFRNTAFIKDMFSSAMEVARFEGAQIKTVSGIRGEIKRALSKPEGHFRATFEDKILLSDIIILRSWYPVHIKKFYNPVTSLLLKNKSEWKGLRLTGQIRASQGLETPTNADSAYKKVERVERRFNGLKVPSSVQKTLPFKSQIHQMKPQKKKTYMAKRAVVLGGEEKKARSFMQKVFTISNAKDEKKKAKKQDERKERLKKLAKIEETKTEKEKERKKKFFSEHGKKRQFDSSADGASSKKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2C2</accession>
    <name>C5E2C2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0H03762p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0H03762g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CBP3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556045.1">
        <property type="nucleotide sequence ID" value="XM_002555999.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2C2"/>
    <dbReference type="FunCoup" id="C5E2C2">
        <property type="interactions" value="123"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2C2"/>
    <dbReference type="GeneID" id="8294358"/>
    <dbReference type="KEGG" id="lth:KLTH0H03762g"/>
    <dbReference type="eggNOG" id="KOG2873">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051390_1_0_1"/>
    <dbReference type="InParanoid" id="C5E2C2"/>
    <dbReference type="OMA" id="FSQWFQI"/>
    <dbReference type="OrthoDB" id="10253878at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034551">
        <property type="term" value="P:mitochondrial respiratory chain complex III assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021150">
        <property type="entry name" value="Ubiq_cyt_c_chap"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007129">
        <property type="entry name" value="Ubiqinol_cyt_c_chaperone_CPB3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12184">
        <property type="entry name" value="UBIQUINOL-CYTOCHROME C REDUCTASE COMPLEX ASSEMBLY FACTOR 1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12184:SF1">
        <property type="entry name" value="UBIQUINOL-CYTOCHROME-C REDUCTASE COMPLEX ASSEMBLY FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03981">
        <property type="entry name" value="Ubiq_cyt_C_chap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquinol-cytochrome c chaperone" evidence="3">
        <location>
            <begin position="146"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="312"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR30183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="336" mass="39098" checksum="2D14DC01A37363EC" modified="2009-07-28" version="1">MFRTLFVANKASSCSASTPLKAFRCLARAGYSVKANREEAKELESPEELAQKSKLKAKPIDYSNNAPVNPKKKRPFLSGSKYESRVYQLPKWKEALGEIVIRSFNLDMDKVRAGPVAGSYYYALCKEQGLQFENEPLSTTARFFYEDLKLPRTFSQWFQITILHEWILFVRMRAMPFKYGRNYQQKLVDRTFSDIELRLFDEMNVNSGRIADQYLKDFNSQLRGAVFAYDEGLFTDDSTLAAAIWRNLFGGRKNVDMVHLEATVRYVRAQIYVLSKMSDREFAMGKFKFVSPDETVYRLTAQQEAEMKENVKRKYEEMDRNPDLLPSERSKLSYDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2L3</accession>
    <name>C5E2L3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">KLTH0H05896p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0H05896g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC12A transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556136.1">
        <property type="nucleotide sequence ID" value="XM_002556090.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2L3"/>
    <dbReference type="FunCoup" id="C5E2L3">
        <property type="interactions" value="195"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2L3"/>
    <dbReference type="GeneID" id="8294451"/>
    <dbReference type="KEGG" id="lth:KLTH0H05896g"/>
    <dbReference type="eggNOG" id="KOG1288">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001883_4_0_1"/>
    <dbReference type="InParanoid" id="C5E2L3"/>
    <dbReference type="OMA" id="NIKYWRP"/>
    <dbReference type="OrthoDB" id="2020542at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015379">
        <property type="term" value="F:potassium:chloride symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006884">
        <property type="term" value="P:cell volume homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055064">
        <property type="term" value="P:chloride ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055075">
        <property type="term" value="P:potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034486">
        <property type="term" value="P:vacuolar transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000013">
        <property type="entry name" value="Solute carrier family 12 member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018491">
        <property type="entry name" value="SLC12_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004842">
        <property type="entry name" value="SLC12A_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11827:SF72">
        <property type="entry name" value="GH08340P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11827">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 12, CATION COTRANSPORTERS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03522">
        <property type="entry name" value="SLC12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="91"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="126"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="191"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="219"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="281"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="314"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="355"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="424"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="463"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="9">
        <location>
            <begin position="64"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="domain" description="SLC12A transporter C-terminal" evidence="10">
        <location>
            <begin position="549"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="930"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="35"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="960"/>
            <end position="974"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR30274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1123" mass="124833" checksum="99FF23330581F035" modified="2009-07-28" version="1">MSKFFEIPGSHKPTKKGEEQALSRPQNESLSYFNYGSTKRVPKTSSKLDPDNPNKSKLGTFDGVFVPTTLNVLSILMFLRFGFIIGQMGVLGTLFLLLLSYGINLLTTLSISAISTNGTVRGGGAYYMISRCLGPEFGGSIGLIFFLGQMLNSGMNVVGIIEPIMYNFGTSIDSETPALYPLLRRGYWWEFGYASFILLICLIISMVGSAMVSRAGKVLFWLLLFSTFSIPLSALLVAPFNDGEVNYTGLSSETLKMNLYPQFTKHAAGSLLKGKETFNDLFGIFFPATAGIFAGAGMSSELRKPSKSIPKGTLWGLALTFACYLLVIVTIGSCVPRDSLHKDVQIIQTVSASQIVILVGELSTSIFSIIVGIVGAAYVLEAIAKDSIFPGISIFEKKPLYSVLFSWFLTQLCLFSDVNKIATIITMAFLMTFLVMNLACFLLEISSAPNFRPSFNYFDRYTAFIGGSLSLIAMFIVDMISAAVVILTISTIFVVIHFFSPPKPWGDVSQNIIYHQVRKYLLRLRQDNVKYWRPQILLLVDNPRTSWNLIKFCNHLKKGGLYVLGHVTVSKTFQGEYEEMRRQTKAWVKIRDMANIKAFVQVGTGPTLPWGVRNVFLGSGLGGMKPNITVIGFFDLKNYHETRNKASDALERGKYSKNFQGLRAARRDDGSVAVEIPDNVGLLPTDDCKNERKIKVQQWVQIIEDLSLMHSNIAVARGFLDLSLPSKGDHSKKKRFIDLYPIQMSAKVILEENSSEGILATNFDTYTLILQLGAILVTVPSWKKTHQLRVVVFVESESERHDENKRITNLLGILRIEAEVVVLSLDQFRVYNTIIKGDPIQVQEVSDVLKDDPWWAELVDARTTHKTRRRFSAGAHPMAEIPKNARRTTPKYNVSQLQRLGVSMTMTSNFLKANLSITDLSDDEDTYMDQDSEVLGDSSSKINSDREDNNQRPATNSSRDNLRPKTSRVSDERSVRSLMPVFSSDAIPKTKVIEDASGDEPSLVPVVEGAELHPPAFDKRTTKNRVQTPLSPCQSSESLLADLRGLTFNDVPSRAQHLILNDVMSQVSKDSDLIFSTLPIPPLNTHKSEEGSLEYVENLHVWLAGLPPTMLINSKSTTVTTAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2M0</accession>
    <name>C5E2M0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0H06050p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0H06050g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556143.1">
        <property type="nucleotide sequence ID" value="XM_002556097.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2M0"/>
    <dbReference type="FunCoup" id="C5E2M0">
        <property type="interactions" value="815"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2M0"/>
    <dbReference type="GeneID" id="8294458"/>
    <dbReference type="KEGG" id="lth:KLTH0H06050g"/>
    <dbReference type="eggNOG" id="KOG2190">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022670_9_0_1"/>
    <dbReference type="InParanoid" id="C5E2M0"/>
    <dbReference type="OMA" id="MQVVPYS"/>
    <dbReference type="OrthoDB" id="1937934at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00105">
        <property type="entry name" value="KH-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22438">
        <property type="entry name" value="KH-I_PCBP_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="5">
        <location>
            <begin position="54"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="5">
        <location>
            <begin position="136"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="5">
        <location>
            <begin position="303"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR30281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="392" mass="43240" checksum="74E8A496C4ED54E6" modified="2009-07-28" version="1">MGTSEKNEELPNSPNVLKRKKEDCQEELLEAEIKRVALDDTEETNDPGNEINKDYIHLRMLCLVKQASMIVGPGGEKISRMKEETNTRINVSDNIRGVPERVIFIRGRCEDVAKVFGMVVRAINGETDGESSEQSATLTLNILIPHHMMGCVIGRQGSRLREIEELSAAKLMAGPQTLPMSNDRILCVSGVADAIHIATYYVGQTILNHKATFASKKCIFYLPSLLHSVLVNSYGVSLQHQQQHQYRPGENGKKRLHRYSASPDFGYAYPGVPSAPHMAVHNVRVADTLAAATIPPALAPQLSLIQQEVYIDDNYVGNVIGKGGKHINSIKETTGCSIQIDDHVEGCSERKILIRGTPIGSQTAILLINNKIEMDRRNKQSIGDKLENPLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2Y5</accession>
    <name>C5E2Y5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">KLTH0H08844p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0H08844g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556258.1">
        <property type="nucleotide sequence ID" value="XM_002556212.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2Y5"/>
    <dbReference type="FunCoup" id="C5E2Y5">
        <property type="interactions" value="359"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2Y5"/>
    <dbReference type="GeneID" id="8294583"/>
    <dbReference type="KEGG" id="lth:KLTH0H08844g"/>
    <dbReference type="eggNOG" id="KOG0752">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015166_10_1_1"/>
    <dbReference type="InParanoid" id="C5E2Y5"/>
    <dbReference type="OMA" id="YKMSVPK"/>
    <dbReference type="OrthoDB" id="270584at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24089">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 10" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="13"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="115"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="223"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR30396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="317" mass="34720" checksum="6F9FFE2014A20941" modified="2009-07-28" version="1">MAQENGWTRALRSDASVAFIAGGVAGAVSRTVVSPFERVKILLQVQSSTHAYNQGIFGAVRQVYAEEGVPGLLRGNGLNCVRIFPYSAVQFVVYEFCKKQWFERGAADGRAPQQMQNWQRLLSGALCGGCSVLATYPLDLVRTRLSIQTANLASLQRAKAANVAKPPGVWELLENTYKNEGGVVGLYRGVWPTSIGVVPYVALNFAVYEQLREFIPPSVDPGWASVFKLTIGALSGGVAQTITYPFDLLRRRFQVLAMGQNELGFRYKSVTDALVTIGRTEGFKGYYKGLTANLFKVVPSTAVSWVVYEAVRDLMQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2Z2</accession>
    <name>C5E2Z2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0H08998p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0H08998g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556265.1">
        <property type="nucleotide sequence ID" value="XM_002556219.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2Z2"/>
    <dbReference type="FunCoup" id="C5E2Z2">
        <property type="interactions" value="186"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2Z2"/>
    <dbReference type="GeneID" id="8294590"/>
    <dbReference type="KEGG" id="lth:KLTH0H08998g"/>
    <dbReference type="eggNOG" id="KOG4558">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058669_1_0_1"/>
    <dbReference type="InParanoid" id="C5E2Z2"/>
    <dbReference type="OMA" id="IHQMAPW"/>
    <dbReference type="OrthoDB" id="5394411at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024624">
        <property type="entry name" value="Pyridox_Oxase_Alr4036_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28243">
        <property type="entry name" value="AGL049CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28243:SF1">
        <property type="entry name" value="PYRIDOXAMINE 5'-PHOSPHATE OXIDASE ALR4036 FAMILY FMN-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12766">
        <property type="entry name" value="Pyridox_oxase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyridoxamine 5'-phosphate oxidase Alr4036 family FMN-binding" evidence="2">
        <location>
            <begin position="5"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="146"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="223"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR30403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="311" mass="35387" checksum="5B13CABDADF74C9E" modified="2009-07-28" version="1">MIHQMAPWVPQFIQSWKNHSQPFAPFTFATLDMQSNRPKCRTVIFRDFLFHDKRSNVLTFTTDMRGDKVKEILGSGAETAPFEACFYFPSSWEQYRFSGKCFVVSRQSKPLITHTFNGAESGAIEYPILSPSIYGDAHVLYQHEHVDESEDEHVQNDEEHNGTSADNETEPDVASVRPELSRTSTRSSGPAAHHAVLTPAEYAPPTPKEWDLELARQWADLSRSTKAQFRKPHPGTPVTSETSKQLDKIQRGVDGAKENTGFDNFGVVCLCIDEVDFLNLKDGRGGERWKFRRHVEEDSHLESWIEEEVCP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-04-05" modified="2025-06-18" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5E325</accession>
    <name>MIC60_LACTC</name>
    <protein>
        <recommendedName>
            <fullName>MICOS complex subunit MIC60</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Mitofilin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">MIC60</name>
        <name type="ordered locus">KLTH0H09724g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane. Plays a role in keeping cristae membranes connected to the inner boundary membrane. Also promotes protein import via the mitochondrial intermembrane space assembly (MIA) pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the mitochondrial contact site and cristae organizing system (MICOS) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MICOS complex subunit Mic60 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556298.1">
        <property type="nucleotide sequence ID" value="XM_002556252.1"/>
    </dbReference>
    <dbReference type="PDB" id="7PUZ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.84 A"/>
        <property type="chains" value="A=206-382"/>
    </dbReference>
    <dbReference type="PDBsum" id="7PUZ"/>
    <dbReference type="AlphaFoldDB" id="C5E325"/>
    <dbReference type="SMR" id="C5E325"/>
    <dbReference type="FunCoup" id="C5E325">
        <property type="interactions" value="224"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E325"/>
    <dbReference type="GeneID" id="8294623"/>
    <dbReference type="KEGG" id="lth:KLTH0H09724g"/>
    <dbReference type="eggNOG" id="KOG1854">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008024_2_0_1"/>
    <dbReference type="InParanoid" id="C5E325"/>
    <dbReference type="OMA" id="DYATDAY"/>
    <dbReference type="OrthoDB" id="10261039at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061617">
        <property type="term" value="C:MICOS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042407">
        <property type="term" value="P:cristae formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019133">
        <property type="entry name" value="MIC60"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15415:SF7">
        <property type="entry name" value="MICOS COMPLEX SUBUNIT MIC60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15415">
        <property type="entry name" value="MITOFILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09731">
        <property type="entry name" value="Mitofilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0175">Coiled coil</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0496">Mitochondrion</keyword>
    <keyword id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0809">Transit peptide</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="transit peptide" description="Mitochondrion" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000406657" description="MICOS complex subunit MIC60">
        <location>
            <begin position="25"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="topological domain" description="Mitochondrial matrix" evidence="2">
        <location>
            <begin position="25"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="topological domain" description="Mitochondrial intermembrane" evidence="2">
        <location>
            <begin position="62"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="313"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="236"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="340"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="365"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="370"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="7PUZ"/>
        </source>
    </evidence>
    <sequence length="527" mass="58100" checksum="A1F5038E7FA688AD" modified="2009-07-28" version="1" precursor="true">MLRSRATINCVKSGRAGRALSRGMATFEGQPAARPNRLSKLLVRVGLATVGFYVGGVTLSLNNDQFGELFCDNVPLAESLVEMYEEFRDEKMQASRMSLDELKQKFGELGTKVDRIPNRGADPALTSQAVAALPASKEVRLEDESLVKLRLPEVEQLGSCKRATPLVESVNAAVAAVNEQSLLLPEDTYNAVHDAFTKLKSALQAINEDIRTNVAESVAVQYGQASKDLHESFEIRAKSREVELTQQFLNEFNAFKAQLEKHSSEELASALKANEQALLAKQSNEVALLSMKQVEEFTKILSEKLDQERQGRLSKLEALNGSVQELAEAVDQVDTLVMKSEVLSQLSLLTTLLKNKLHAGDESSVKIDSELARLKTLCDILPGRPSKCCSKNPQLLDVVVSQLDSLASQQLILSNEQLYNRWTLLQKDLSTSSLLPPNAGILGHISAKIFGFFLFNKNGAPVDNDIDSVIARVGQNLRLSKLDKAVEEVVALKGWPRVLCDEWVQEARKKLEIETLIDALDCEIRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>C5E332</accession>
    <name>C5E332_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0H09900p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0H09900g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556305.1">
        <property type="nucleotide sequence ID" value="XM_002556259.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E332"/>
    <dbReference type="FunCoup" id="C5E332">
        <property type="interactions" value="53"/>
    </dbReference>
    <dbReference type="GeneID" id="8294631"/>
    <dbReference type="KEGG" id="lth:KLTH0H09900g"/>
    <dbReference type="eggNOG" id="ENOG502RZH0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079711_0_0_1"/>
    <dbReference type="InParanoid" id="C5E332"/>
    <dbReference type="OMA" id="MEELYVY"/>
    <dbReference type="OrthoDB" id="4070108at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="240"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="293"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="313"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR30443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="324" mass="36209" checksum="7B5D40AEE8F34659" modified="2009-07-28" version="1">MQEIYVPIGEDQRVKLTLVSTFQHIGQVRKYLHSIQDTFSVELDTCDTITQNLKIQCVSHRACRSCPLYILEFDDVSDGYALWKSAGDWALGTVLSSLFAARPASKHRAARLETQLAAHATRKHDPEFLAQALQKLNVDWDAPHARFEFDFALFVRLLDEAVAPEIGARTCDSYLELVSPLQYKSLTTMVVLRTKVAVSKQHVECSLKDYDARMRGLALARRAADRADSVALDRSHEASVLSDAAERSPSPPVSTSDDDAYLLSSSQQLLTNFQKHFRGMAETFETFDVKRAAQSPRRLAAPSDCWKVSKPVKNGKSKPSKVHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5E338</accession>
    <name>C5E338_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptide hydrolase</fullName>
            <ecNumber evidence="6">3.4.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0H10054g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase M28 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.-.-" evidence="6"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556311.1">
        <property type="nucleotide sequence ID" value="XM_002556265.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E338"/>
    <dbReference type="FunCoup" id="C5E338">
        <property type="interactions" value="60"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E338"/>
    <dbReference type="MEROPS" id="M28.001"/>
    <dbReference type="GeneID" id="8294638"/>
    <dbReference type="KEGG" id="lth:KLTH0H10054g"/>
    <dbReference type="eggNOG" id="KOG2195">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024336_0_1_1"/>
    <dbReference type="InParanoid" id="C5E338"/>
    <dbReference type="OMA" id="PNYEYEV"/>
    <dbReference type="OrthoDB" id="10013407at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008235">
        <property type="term" value="F:metalloexopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02130">
        <property type="entry name" value="PA_ScAPY_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000093">
        <property type="entry name" value="Peptide hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045175">
        <property type="entry name" value="M28_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12147:SF17">
        <property type="entry name" value="AMINOPEPTIDASE Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12147">
        <property type="entry name" value="METALLOPEPTIDASE M28 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 6" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 6" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005125158" description="Peptide hydrolase" evidence="6">
        <location>
            <begin position="17"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="7">
        <location>
            <begin position="156"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M28" evidence="8">
        <location>
            <begin position="271"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR30449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="512" mass="56169" checksum="63FCF7141763D9CE" modified="2009-07-28" version="1">MRISTLVATSIGCAQAFVLPQAFFDAFAAPAPVADASLWPHKPYFLKHNVDPDTFPDEIQLEELNATALDLYHIAESSNGTYGHPTRVIGSPGHWKTLGYILHQFDQMRDYYDVTVQSFKALNGKVSHYDLNYTNGEAVPSAQAFSLSPPVEPFVGTLVEIPNLGCSDADFEALEISKDSIALIERGECPFGQKSTLAGKHGFKAVVIYDNDPKSKKGISGTLEKPTKHTVSTIGVSFAVGQKLIAGIELDPEFSLFFYMDSFVKKVKTKNIIADTKHGDPDNIVALGAHTDSVAAGPGINDDGSGTISLLTVAKHLSDYKINNKVRFAFWAAEEEGLVGSNYYVEKLSPEENQKIRLFMDYDMMASPNYQFQVYDANNIDNPRGSEELKNLYIDYYTSHGLNYTLIPFDGRSDYVAFINHGIPGGGIAAGAEGINTDNGKVLDRCYHELCDDVNNLNWEAFLTNTQLIAYSVANYAHSLKEFPERETNTSSVLSSASAPKYPYKGGANLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5E353</accession>
    <name>C5E353_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mitochondrial import inner membrane translocase subunit TIM54</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0H10472g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TIM54 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556326.1">
        <property type="nucleotide sequence ID" value="XM_002556280.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E353"/>
    <dbReference type="FunCoup" id="C5E353">
        <property type="interactions" value="34"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E353"/>
    <dbReference type="GeneID" id="8294657"/>
    <dbReference type="KEGG" id="lth:KLTH0H10472g"/>
    <dbReference type="eggNOG" id="ENOG502QPMQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039097_0_0_1"/>
    <dbReference type="InParanoid" id="C5E353"/>
    <dbReference type="OMA" id="RNWMIFF"/>
    <dbReference type="OrthoDB" id="5598305at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050187">
        <property type="entry name" value="Lipid_Phosphate_FormReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021056">
        <property type="entry name" value="Mt_import_IM_translocase_Tim54"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12358:SF101">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12358">
        <property type="entry name" value="SPHINGOSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11711">
        <property type="entry name" value="Tim54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0811">Translocation</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="262"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="137"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="265"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="288"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR30464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="482" mass="54323" checksum="DE5AEBBC020E169D" modified="2009-07-28" version="1">MSSSENPKPPQDGPKILKKPGYSNPAFKAMGIPTLRLPSRNWMIFWTVLTVSVSGIVYDKQQQKKIVKKWCKTVEPLSEEKFPVNEVPRKVTVFVAPPPSDYLDTSMSVWRRFVKPVLYSSGIDYEVFTEEKQGPIRAEVAERIRELRRELIKHEEDIKRLNDEKKIWNRCKRALTGKLQHMRSSEGLDQEIEKAAAEKFKAEFDYKNLLGVYYKNKNKSNKVVAEDALVSSPEMAGGVICIGRGAYKEYIEGVHEGILGPLEPTATTNAQTEAASEKAPALPEVLSGATQTEAKVENSNEVSENATPNTETALTNESAEAESAKDSDLDPEGSAKPKPVPKPYISPKGYESAELPQELSSPNVARDLITGIPALFYQPILVIPMPNLVGFLNIPERIYRYYRRRYLAEEYCKAAASCVLQKVRPFDGTTDLEIAKSEENDWPKKWVQQGLEKDSPWVQELQGDRRVLEKLTVYDPSLIKEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2010-11-02" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>C5E381</accession>
    <name>MIC12_LACTC</name>
    <protein>
        <recommendedName>
            <fullName>MICOS complex subunit MIC12</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Altered inheritance of mitochondria protein 5, mitochondrial</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Found in mitochondrial proteome protein 51</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">AIM5</name>
        <name type="synonym">FMP51</name>
        <name type="ordered locus">KLTH0H11088g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the mitochondrial contact site and cristae organizing system (MICOS) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MICOS complex subunit Mic12 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E381"/>
    <dbReference type="FunCoup" id="C5E381">
        <property type="interactions" value="55"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E381"/>
    <dbReference type="KEGG" id="lth:KLTH0H11088g"/>
    <dbReference type="eggNOG" id="ENOG502S8MU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_164154_0_0_1"/>
    <dbReference type="InParanoid" id="C5E381"/>
    <dbReference type="OMA" id="DIWNEQI"/>
    <dbReference type="OrthoDB" id="4037694at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061617">
        <property type="term" value="C:MICOS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044284">
        <property type="term" value="C:mitochondrial crista junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042407">
        <property type="term" value="P:cristae formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031463">
        <property type="entry name" value="Mic12"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17050">
        <property type="entry name" value="AIM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0496">Mitochondrion</keyword>
    <keyword id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000399891" description="MICOS complex subunit MIC12">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="4"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="102" mass="11579" checksum="544032C590F6E94C" modified="2009-07-28" version="1">MSKVLKLTSVTLAASSLAAAGYFYAFDRDSYHYKNASWKRIGDHVQGILDRKEDIAVQRTSSEARDVTVRPIKETMKDLWNEQVRNTAQWVYSFGSNTSTKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3A3</accession>
    <name>C5E3A3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein SYM1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0H11660g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the peroxisomal membrane protein PXMP2/4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556376.1">
        <property type="nucleotide sequence ID" value="XM_002556330.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3A3"/>
    <dbReference type="FunCoup" id="C5E3A3">
        <property type="interactions" value="618"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3A3"/>
    <dbReference type="GeneID" id="8294710"/>
    <dbReference type="KEGG" id="lth:KLTH0H11660g"/>
    <dbReference type="eggNOG" id="KOG1944">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049109_8_1_1"/>
    <dbReference type="InParanoid" id="C5E3A3"/>
    <dbReference type="OMA" id="CAPTMIG"/>
    <dbReference type="OrthoDB" id="430207at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007248">
        <property type="entry name" value="Mpv17_PMP22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266:SF17">
        <property type="entry name" value="PROTEIN MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04117">
        <property type="entry name" value="Mpv17_PMP22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR30514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="200" mass="22759" checksum="5441EE87654D0B80" modified="2009-07-28" version="1">MSMLLNFYTSSLKKNPKTTNAIMTGSLFGIGDAIAQIGFPSSHSKAQGYDIARTVRAVTYGSLIFSFIGDKWFKVLNNKVRFSNRPTNHWSNLPLRVGTDQLLFAPLCIPFYFGILTLMEGKSLKHADTKVRAVWWDILKTNWMVWPLFQLINFSIIPVQHRLLAVNVLAIFWNTFLSFRNSEASHADHQFPVNNPPVPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3C2</accession>
    <name>C5E3C2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0H12144p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0H12144g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556395.1">
        <property type="nucleotide sequence ID" value="XM_002556349.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3C2"/>
    <dbReference type="FunCoup" id="C5E3C2">
        <property type="interactions" value="778"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3C2"/>
    <dbReference type="GeneID" id="8294732"/>
    <dbReference type="KEGG" id="lth:KLTH0H12144g"/>
    <dbReference type="eggNOG" id="KOG3712">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016699_0_0_1"/>
    <dbReference type="InParanoid" id="C5E3C2"/>
    <dbReference type="OMA" id="MIWLREN"/>
    <dbReference type="OrthoDB" id="10056949at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003150">
        <property type="entry name" value="DNA-bd_RFX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039779">
        <property type="entry name" value="RFX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057321">
        <property type="entry name" value="RFX1-4/6/8-like_BCD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12619">
        <property type="entry name" value="RFX TRANSCRIPTION FACTOR FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12619:SF5">
        <property type="entry name" value="TRANSCRIPTION FACTOR RFX4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25340">
        <property type="entry name" value="BCD_RFX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02257">
        <property type="entry name" value="RFX_DNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51526">
        <property type="entry name" value="RFX_DBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RFX-type winged-helix" evidence="3">
        <location>
            <begin position="269"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="25"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="38"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR30533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="740" mass="81203" checksum="60FD47EB3D01F848" modified="2009-07-28" version="1">MSDRQPSAGDEKPMPPPLQPLLTKNGSAGSTGSAGSSGTSGTGGASGMSGVGGSSGANSNSAGGPAGAGVLQIPPRADSVPPLAAASQPYQTQHSFLSAAFQQPQRGYAPNYGLPMSANKQQPMPPYTASYTPPLGPGYLPSPIPQLMLNEKSMDLHRGFQQQFPVFHAPQPHFAPTMESDSSMKKRRLLDYSDQRSEAVKDRGATKKNGNEAILRDLAMENKARPLSECALTVREAEIAVLNMDPTTHSKSEIQAAEQVRERERQVYALTWLMNTCVEEKNSFVPRGRIFAQYAACCAQNNLKPLSQATLGKLIRTIFPDLTTRRLGMRGQSKYHYCGLNLIGSSRSASPVPSLGNSDLGGVNNVQNISNVNVGVNNSSNNSLYSSIQGQGVAALSSETPSIFQESHEARSDKWEDVAHWDCLRIADELFHNVSTVDVNVSLEIPSINSYLPPNTDIDIAASLECMYKVHCNTVHENISFAKFDALTNTLESFSSGSISPQMFNLFISEALYDWVQQADLVTHKATAKSLFKFIKEFATLPSSVSEKLASFAHSYPEMISNANIDLPVPMVRNKVSIAEKFAKLVKRTTRVIRTAKSASKAYRTATSREEMYMDWKKLIKVKDICRTELYCCRNDEASLNEITSFIIQMIEGFFDRDEHSISEDFPMELAAQLLTFLKKIRDHPVSVVIACTNVVTTGVIRELSIMGSEHAGSWWAIKTFIDEWLYWCDEVGTFMISPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3E3</accession>
    <name>C5E3E3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0H12694p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0H12694g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556416.1">
        <property type="nucleotide sequence ID" value="XM_002556370.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3E3"/>
    <dbReference type="FunCoup" id="C5E3E3">
        <property type="interactions" value="481"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3E3"/>
    <dbReference type="GeneID" id="8294757"/>
    <dbReference type="KEGG" id="lth:KLTH0H12694g"/>
    <dbReference type="eggNOG" id="KOG3771">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072096_0_0_1"/>
    <dbReference type="InParanoid" id="C5E3E3"/>
    <dbReference type="OMA" id="TRFCAYF"/>
    <dbReference type="OrthoDB" id="446293at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031097">
        <property type="term" value="C:medial cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990528">
        <property type="term" value="C:Rvs161p-Rvs167p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051666">
        <property type="term" value="P:actin cortical patch localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097320">
        <property type="term" value="P:plasma membrane tubulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07591">
        <property type="entry name" value="BAR_Rvs161p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000014">
        <property type="entry name" value="Protein hob3, variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046982">
        <property type="entry name" value="BIN3/RVS161-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037429">
        <property type="entry name" value="Rvs161/Hob3_BAR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47174">
        <property type="entry name" value="BRIDGING INTEGRATOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47174:SF3">
        <property type="entry name" value="BRIDGING INTEGRATOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03114">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00721">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51021">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BAR" evidence="5">
        <location>
            <begin position="15"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="137"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR30554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="265" mass="30336" checksum="4B9C9179DACA5B61" modified="2009-07-28" version="1">MSWEGFKKAINRAGNSVLVKEVDKTIDKEYDIEERRYRTLERAGKELQKEAKGYLDSLRAVTASQVTIAEVISNLYDDSKTVSGGGYNVGNYYLQCVRDYDSETVKQLDGPFRETVLDPITKFSTYFSEIGDAIKKRDHKKQDYDAAKAKVRRLIDKPAKDASKLPRAEKELALAKDIFDNLNEQLKTELPQLVSLRVPYYDPSFEALVKIQLRFCTEGYTRLAQIQQYLDQQSRDDYANGQLDEKVADLLQQMTNLNICALGFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3L9</accession>
    <name>C5E3L9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Deoxyhypusine synthase</fullName>
            <ecNumber evidence="6">2.5.1.46</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0H14652g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[eIF5A protein]-L-lysine + spermidine = [eIF5A protein]-deoxyhypusine + propane-1,3-diamine</text>
            <dbReference type="Rhea" id="RHEA:33299"/>
            <dbReference type="Rhea" id="RHEA-COMP:10143"/>
            <dbReference type="Rhea" id="RHEA-COMP:10144"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57484"/>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
            <dbReference type="ChEBI" id="CHEBI:82657"/>
            <dbReference type="EC" id="2.5.1.46"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; eIF5A hypusination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the deoxyhypusine synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.46" evidence="6"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556492.1">
        <property type="nucleotide sequence ID" value="XM_002556446.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3L9"/>
    <dbReference type="FunCoup" id="C5E3L9">
        <property type="interactions" value="739"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3L9"/>
    <dbReference type="GeneID" id="8294855"/>
    <dbReference type="KEGG" id="lth:KLTH0H14652g"/>
    <dbReference type="eggNOG" id="KOG2924">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039781_0_0_1"/>
    <dbReference type="InParanoid" id="C5E3L9"/>
    <dbReference type="OMA" id="HSIINAN"/>
    <dbReference type="OrthoDB" id="294378at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034038">
        <property type="term" value="F:deoxyhypusine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.910.10:FF:000003">
        <property type="entry name" value="Deoxyhypusine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.910.10">
        <property type="entry name" value="Deoxyhypusine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002773">
        <property type="entry name" value="Deoxyhypusine_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036982">
        <property type="entry name" value="Deoxyhypusine_synthase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00321">
        <property type="entry name" value="dhys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11703">
        <property type="entry name" value="DEOXYHYPUSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11703:SF0">
        <property type="entry name" value="DEOXYHYPUSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01916">
        <property type="entry name" value="DS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0386">Hypusine biosynthesis</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00020607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR30630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="380" mass="41847" checksum="5F6B13292D7D1E68" modified="2009-07-28" version="1">MSALNEKLPDTLNDAVLKASVSISDDHVKVQGIDYSKSEAVDMRASDLVESMKTMGFQASALGQGCEIINKMRSWRGKHIDELEEHERKGSFDEEGRQKTTIFLGYTSNLISSGLRETIRYLVQHNQVSAIVATAGGIEEDLIKCLAPTYLGEFTLKGANLRDKGLNRIGNLLVPNDNYCKFEEWIVPILDKMLEEQEEYVTKHGNQCLDASTDVDVPIWTPSKLIDRLGKEINDESSVLYWAHKNKIPVFCPAVTDGSIGDMLFFHTFKSSPSQLRLDIVSDIRKINCMSMEASKAGMLLLGGGLIKHHIANACLMRNGADYAVYINTGQEFDGSDAGARPDEAVSWGKIKAEAESVKIYADATVVFPLVVAATFASGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3M5</accession>
    <name>C5E3M5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0H14806p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0H14806g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CENP-O/MCM21 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556498.1">
        <property type="nucleotide sequence ID" value="XM_002556452.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3M5"/>
    <dbReference type="FunCoup" id="C5E3M5">
        <property type="interactions" value="98"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3M5"/>
    <dbReference type="GeneID" id="8294862"/>
    <dbReference type="KEGG" id="lth:KLTH0H14806g"/>
    <dbReference type="eggNOG" id="ENOG502RXWX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068734_0_0_1"/>
    <dbReference type="InParanoid" id="C5E3M5"/>
    <dbReference type="OMA" id="NHSFATI"/>
    <dbReference type="OrthoDB" id="10050372at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018464">
        <property type="entry name" value="CENP-O"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09496">
        <property type="entry name" value="CENP-O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0137">Centromere</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="69"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="11"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR30636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="346" mass="39656" checksum="5221B3E85F1F9404" modified="2009-07-28" version="1">MVEEEDNDQYINSLNAEILQLKNKCRVLEDELQAQKKGVSEVPVEFEGLFQKNPELKHTLSQKIFKETDSFPQTPYSSPKKRPLKSSAAQLSNLSPDIPESEWVLKTQVPVEHKFFDSAVADLIETDILTSPSKRKRAAQSRAHEELVQKVQIENIYRMLGISTFPVVDPSDLEGTEEDEINIKRKMLGLRLELFNELERSFEPPYYILFKQNPKNSYWNIFRHTVPSYVGVEQMFQQVRSNGLLANQSEIYTFAKKVYKSLLSISVKTQIFRRTEKKEAISQLEIDPSCTSVSFHIEKLGCFVKLKLDATDVIACSCIPHENVSWNAQMLGPVVSLPDRIESLVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3P6</accession>
    <name>C5E3P6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0H15312p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0H15312g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNU66/SART1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556519.1">
        <property type="nucleotide sequence ID" value="XM_002556473.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3P6"/>
    <dbReference type="FunCoup" id="C5E3P6">
        <property type="interactions" value="899"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3P6"/>
    <dbReference type="GeneID" id="8294885"/>
    <dbReference type="KEGG" id="lth:KLTH0H15312g"/>
    <dbReference type="eggNOG" id="KOG2217">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018358_0_0_1"/>
    <dbReference type="InParanoid" id="C5E3P6"/>
    <dbReference type="OMA" id="FNGRHYR"/>
    <dbReference type="OrthoDB" id="5583at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000481">
        <property type="term" value="P:maturation of 5S rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045347">
        <property type="entry name" value="HIND"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005011">
        <property type="entry name" value="SNU66/SART1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14152">
        <property type="entry name" value="SQUAMOUS CELL CARCINOMA ANTIGEN RECOGNISED BY CYTOTOXIC T LYMPHOCYTES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14152:SF5">
        <property type="entry name" value="U4_U6.U5 TRI-SNRNP-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19252">
        <property type="entry name" value="HIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03343">
        <property type="entry name" value="SART-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="21"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="226"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="306"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="386"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="539"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="27"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="315"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="543"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="559"/>
            <end position="569"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR30657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="569" mass="64868" checksum="FC25CBB4E2C42C94" modified="2009-07-28" version="1">MAHEISLSLEETNKLRLSLGLKPINAKADDKKDVSRTQGKENKREPNDKSEGAGADSHGYERSAFIDDKVSKLRWNLKRAKSKISSPVTLVDEDDAADGQDWLTSVGTRKKPKRTLDKVYEEQEEASQDVELGMKISHRMSDFSGRRNVILTLQEASVDAEDKEDVLVDEGVTQDLQQAKRLELKRMNQDRKRKGVGANFVSENEDQEATSLRLVNGKIVDEETLTLQQEPEASQDNRIKASIEPSASEDEERQTTDFAPIKIKKRKKLKSSSLSQKRSRPVSQIPKVTLVNEDAEDPAEEELQGFLKIRKSRSSKADSSERSAEQIAIEARKEALERKERATKVANVKISQEMVLDENSVFLHNLKKDLISNKSSSHKFEKLNLKAPASDEAQSVPGSKATDERASVFEKKDEPQFHEGLASTLSFLKERNVLPQQPKASTASLRSKDLELLALKQKLETRKVKEKFKEELSQDKIQYSREELDRIEELQHQEIAKRNQSLQKERLAGYNPEVKLTYKDERGNELTTKEAYKQLSQAFHGTHSNRRKLTKAQRKVEQRNQQNQHENFM</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-03-08" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3S0</accession>
    <name>OSW5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName>Outer spore wall protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">OSW5</name>
        <name type="ordered locus">KLTH0H15840g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in spore wall assembly.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the OSW5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3S0"/>
    <dbReference type="SMR" id="C5E3S0"/>
    <dbReference type="FunCoup" id="C5E3S0">
        <property type="interactions" value="14"/>
    </dbReference>
    <dbReference type="KEGG" id="lth:KLTH0H15840g"/>
    <dbReference type="HOGENOM" id="CLU_1949205_0_0_1"/>
    <dbReference type="InParanoid" id="C5E3S0"/>
    <dbReference type="OrthoDB" id="4035996at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030435">
        <property type="term" value="P:sporulation resulting in formation of a cellular spore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031430">
        <property type="entry name" value="Osw5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17062">
        <property type="entry name" value="Osw5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0749">Sporulation</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000405524" description="Outer spore wall protein 5">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="2"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="2">
        <location>
            <begin position="22"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="2">
        <location>
            <begin position="48"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="129" mass="13958" checksum="D94327D056611E87" modified="2009-07-28" version="1">MSFTSIFIALLYLGGFLIILVTASAVILPLLITSLFFATGVVLCGFLSNKSYKLALRAYNLATPHTRSSLQAAADHLAPLDSVETVSPKEELLHKIHHFYKKSDSNVRTPIVVSGSEIPKSVIKVSEGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3T1</accession>
    <name>C5E3T1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0H16082p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0H16082g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556554.1">
        <property type="nucleotide sequence ID" value="XM_002556508.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3T1"/>
    <dbReference type="GeneID" id="8294920"/>
    <dbReference type="KEGG" id="lth:KLTH0H16082g"/>
    <dbReference type="HOGENOM" id="CLU_1482231_0_0_1"/>
    <dbReference type="InParanoid" id="C5E3T1"/>
    <dbReference type="OMA" id="VETCHEE"/>
    <dbReference type="OrthoDB" id="4062750at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR30692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="203" mass="22722" checksum="19573BE692D6425B" modified="2009-07-28" version="1">MKIVKQSRVTRKFKGPRGLDAQAMDCADKLEQRLDLIENSVGELDGVENLVDKLRVISGEMRRLCGTGARCSEFLWQLLNTFAKNEERDTTKEQEVELSACVGSLNQTLSLLSDLEIVCSSSVDRLLSCGLLQNSMKQESVSADISNIGILYSQTMMLIVRTLGIARRFMNLNLEANDFWTDIQSRICMVEGKLHAIQSSNNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>C5DB75</accession>
    <name>C5DB75_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0A00110p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0A00110g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551474.1">
        <property type="nucleotide sequence ID" value="XM_002551428.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DB75"/>
    <dbReference type="STRING" id="559295.C5DB75"/>
    <dbReference type="GeneID" id="8290257"/>
    <dbReference type="KEGG" id="lth:KLTH0A00110g"/>
    <dbReference type="eggNOG" id="ENOG502S64G">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069867_4_1_1"/>
    <dbReference type="InParanoid" id="C5DB75"/>
    <dbReference type="OMA" id="SYPEWNP"/>
    <dbReference type="OrthoDB" id="4036136at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="CDD" id="cd07822">
        <property type="entry name" value="SRPBCC_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019587">
        <property type="entry name" value="Polyketide_cyclase/dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36166">
        <property type="entry name" value="CHROMOSOME 9, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36166:SF1">
        <property type="entry name" value="SRPBCC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10604">
        <property type="entry name" value="Polyketide_cyc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR21032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="157" mass="17622" checksum="7F05D3777C3F8425" modified="2009-07-28" version="1">MKVCTRVEINAPASVVRETLLDFSGHAKWNPFFTSIVLFEHDGHDLSSQIKPGDRLEVTMKDIRSGKVNKFTPTVLANTVNKFKWEGSLIGQWFFSGIHTFEFQEENGGQKCTLLQHETFSGVALPLLKPVLGGAQRNFEAMNNALKKACESQDEHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBJ1</accession>
    <name>C5DBJ1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0A03058p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0A03058g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551590.1">
        <property type="nucleotide sequence ID" value="XM_002551544.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBJ1"/>
    <dbReference type="FunCoup" id="C5DBJ1">
        <property type="interactions" value="408"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBJ1"/>
    <dbReference type="GeneID" id="8290390"/>
    <dbReference type="KEGG" id="lth:KLTH0A03058g"/>
    <dbReference type="eggNOG" id="KOG3100">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075129_2_0_1"/>
    <dbReference type="InParanoid" id="C5DBJ1"/>
    <dbReference type="OMA" id="DDTNKYF"/>
    <dbReference type="OrthoDB" id="427886at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039883">
        <property type="entry name" value="Fcf2/DNTTIP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014810">
        <property type="entry name" value="Fcf2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21686">
        <property type="entry name" value="DEOXYNUCLEOTIDYLTRANSFERASE TERMINAL-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21686:SF12">
        <property type="entry name" value="DEOXYNUCLEOTIDYLTRANSFERASE TERMINAL-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08698">
        <property type="entry name" value="Fcf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fcf2 pre-rRNA processing C-terminal" evidence="4">
        <location>
            <begin position="114"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="19"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="213"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="220"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR21148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="232" mass="26627" checksum="3D753BB7B0332377" modified="2009-07-28" version="1">MASDSIEDLFAELKKLETAGVTHSAPAGESSTPGENEGFDQDKLEFEDDSKIAQQQKTFREIEQRMRKLPRMETGFDSLTLPAGSAVEGTKAVTKVEDPVSILHSRKKDAATKKPSTTEQWFTLPKPELTRELKRDLLVLKHRAALDPKRHYKKEKWTVPERFSVGTIVEDKTEFYSSRLNNKQRKSTMVGELMDSENTNKYFKRKYTEVQAKKTSGAKGHYRKVREKRNKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBR1</accession>
    <name>C5DBR1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 12">Ribonucleoside-diphosphate reductase</fullName>
            <ecNumber evidence="2 12">1.17.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0A04686g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 12">Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 12">
            <text>a 2'-deoxyribonucleoside 5'-diphosphate + [thioredoxin]-disulfide + H2O = a ribonucleoside 5'-diphosphate + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:23252"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="EC" id="1.17.4.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 12">Belongs to the ribonucleoside diphosphate reductase large chain family.</text>
    </comment>
    <dbReference type="EC" id="1.17.4.1" evidence="2 12"/>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551660.1">
        <property type="nucleotide sequence ID" value="XM_002551614.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBR1"/>
    <dbReference type="FunCoup" id="C5DBR1">
        <property type="interactions" value="1367"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBR1"/>
    <dbReference type="GeneID" id="8290464"/>
    <dbReference type="KEGG" id="lth:KLTH0A04686g"/>
    <dbReference type="eggNOG" id="KOG1112">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000404_1_2_1"/>
    <dbReference type="InParanoid" id="C5DBR1"/>
    <dbReference type="OMA" id="IELPQHM"/>
    <dbReference type="OrthoDB" id="3000483at2759"/>
    <dbReference type="UniPathway" id="UPA00326"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005971">
        <property type="term" value="C:ribonucleoside-diphosphate reductase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004748">
        <property type="term" value="F:ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009263">
        <property type="term" value="P:deoxyribonucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01679">
        <property type="entry name" value="RNR_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.70.20:FF:000001">
        <property type="entry name" value="Ribonucleoside-diphosphate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005144">
        <property type="entry name" value="ATP-cone_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013346">
        <property type="entry name" value="NrdE_NrdA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000788">
        <property type="entry name" value="RNR_lg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013509">
        <property type="entry name" value="RNR_lsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008926">
        <property type="entry name" value="RNR_R1-su_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039718">
        <property type="entry name" value="Rrm1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02506">
        <property type="entry name" value="NrdE_NrdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11573">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE LARGE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11573:SF6">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE LARGE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03477">
        <property type="entry name" value="ATP-cone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02867">
        <property type="entry name" value="Ribonuc_red_lgC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00317">
        <property type="entry name" value="Ribonuc_red_lgN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01183">
        <property type="entry name" value="RIBORDTASEM1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51998">
        <property type="entry name" value="PFL-like glycyl radical enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48168">
        <property type="entry name" value="R1 subunit of ribonucleotide reductase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51161">
        <property type="entry name" value="ATP_CONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00089">
        <property type="entry name" value="RIBORED_LARGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="5 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 12" id="KW-0215">Deoxyribonucleotide synthesis</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-cone" evidence="14">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="825"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="834"/>
            <end position="843"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR21218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="879" mass="98837" checksum="6029D674BA1DBAF8" modified="2009-07-28" version="1">MFVYKRDGRREPVKFDKITARVSRLCYGLDPNHVDAVKITQRIISGVYEGVTTVELDNLAAETCAYMTTIHPDYATLAARIAISNLHKQTTKQFSQVVSDLFHYVNPKNGIHSPMISQEVFDIIQKHKDELNSAIVYDRDFQYNYFGFKTLERSYLLRINGEVAERPQHLIMRVAVGIHGDNIPKVIETYNLMSLKYFTHASPTLFNAGTPHPQMSSCFLVAMKDDSIEGIYDTLKECAMISKTAGGIGLHIHNIRSTGSYIAGTNGTSNGLIPMIRVFNNTARYVDQGGNKRPGAFALYLEPWHSDIFDFVDIRKNHGKEEIRARDLFPALWVPDLFMKRVQENGDWTLFSPSEAPGLSDVYGDEFEALYERYVQEGRGRKTIKAQKLWYAILEAQTETGTPFMLYKDACNRKSNQKNLGVIKSSNLCCEIVEYSAPDETAVCNLASLSLPAFIETSEDGQTQWYNFDKLHEIAKVVTHNLNQVIDRNYYPVESARNSNMRHRPVALGVQGLADAFMILRIPFDSQEAKELNIQIFETIYHAALERSNELAVEHGPYETFEGSPASQGILQFDMWDKKPTDLWDWETLKAKIVKTGLRNSLLLAPMPTASTSQILGNNECFEPYTSNMYSRRVLSGEFQIVNPYLLRDLVDLGIWNDAMKSHIISDNGSIQNLPNIPQKLKDLYKTVWELSQKTIIEMAADRACYIDQSQSLNVHIRAPTMGKLTSMHFYGWKKGLKTGMYYLRTQAASAAIQFTIDKSVASMAGDKEADLQNLHRPLYVPRKMNFSEGAAKDKGIEALRETRESSPSPSVDSSLANSVASLKIEEAKPVTPPATTTDVAAEAKSEATQPENEEFDIYNSKVIACAIDNPGACEMCSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBV5</accession>
    <name>C5DBV5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Protein ROT1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0A05654g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Required for normal levels of the cell wall 1,6-beta-glucan. Involved in a protein folding machinery chaperoning proteins acting in various physiological processes including cell wall synthesis and lysis of autophagic bodies.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the ROT1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551704.1">
        <property type="nucleotide sequence ID" value="XM_002551658.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBV5"/>
    <dbReference type="FunCoup" id="C5DBV5">
        <property type="interactions" value="36"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBV5"/>
    <dbReference type="GeneID" id="8290508"/>
    <dbReference type="KEGG" id="lth:KLTH0A05654g"/>
    <dbReference type="eggNOG" id="ENOG502QQTG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071622_0_0_1"/>
    <dbReference type="InParanoid" id="C5DBV5"/>
    <dbReference type="OMA" id="YKPPQML"/>
    <dbReference type="OrthoDB" id="5327821at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006458">
        <property type="term" value="P:'de novo' protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007118">
        <property type="term" value="P:budding cell apical bud growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019623">
        <property type="entry name" value="Rot1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28090">
        <property type="entry name" value="PROTEIN ROT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28090:SF1">
        <property type="entry name" value="PROTEIN ROT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10681">
        <property type="entry name" value="Rot1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017290">
        <property type="entry name" value="ROT1_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950408" description="Protein ROT1" evidence="11">
        <location>
            <begin position="20"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="230"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR017290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR21262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="249" mass="27457" checksum="AD6B295A38BB9E13" modified="2009-07-28" version="1">MLGALSLISLLAFTATVMGDSDSDALYGTWTSKSNQVFTGPGFFDPVDELLIEPSLPGYSLSFTKDGYFEEAAYRVSGNPQDPHCPTAVLIYQHGTYEVQSNGSLTLTPFEVDGRQLLSSPCTDNGTSVYSRYNQTETFKSFLVQLNNYHGVKELQLYEFDGTAMMPLYLAYQPPVMLPTITLNPTASATSTETGSHNKRSLRGLVKRGLENRYKTSAVKRNREPVNAAAYWWTSVGLIAFGSAVFFVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>C5DC10</accession>
    <name>C5DC10_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0A06930p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0A06930g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551759.1">
        <property type="nucleotide sequence ID" value="XM_002551713.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DC10"/>
    <dbReference type="FunCoup" id="C5DC10">
        <property type="interactions" value="8"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DC10"/>
    <dbReference type="GeneID" id="8290566"/>
    <dbReference type="KEGG" id="lth:KLTH0A06930g"/>
    <dbReference type="eggNOG" id="ENOG502SB2C">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_173479_2_0_1"/>
    <dbReference type="InParanoid" id="C5DC10"/>
    <dbReference type="OMA" id="ICNIYRQ"/>
    <dbReference type="OrthoDB" id="4057220at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1840.10">
        <property type="entry name" value="YNR034W-A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021591">
        <property type="entry name" value="YNR034W-A/EGO2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035098">
        <property type="entry name" value="YNR034W-A/EGO2_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11503">
        <property type="entry name" value="YNR034W-A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160683">
        <property type="entry name" value="YNR034W-A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR21317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="86" mass="9295" checksum="E88C3F1AAD2C958B" modified="2009-07-28" version="1">MSSQSTTKARLAQLVPNAIGLLAFDGNCNVIEASGVAKNRLEDIVQINQAKVDSEGFALLRDGDIQVMVYKHDGETLAVYTQENSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>C5DC37</accession>
    <name>C5DC37_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0A07524p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0A07524g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the CDC50/LEM3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551786.1">
        <property type="nucleotide sequence ID" value="XM_002551740.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DC37"/>
    <dbReference type="FunCoup" id="C5DC37">
        <property type="interactions" value="402"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DC37"/>
    <dbReference type="GeneID" id="8290593"/>
    <dbReference type="KEGG" id="lth:KLTH0A07524g"/>
    <dbReference type="eggNOG" id="KOG2952">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025025_0_1_1"/>
    <dbReference type="InParanoid" id="C5DC37"/>
    <dbReference type="OMA" id="TWNNDQP"/>
    <dbReference type="OrthoDB" id="340608at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005045">
        <property type="entry name" value="CDC50/LEM3_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10926:SF0">
        <property type="entry name" value="CDC50, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10926">
        <property type="entry name" value="CELL CYCLE CONTROL PROTEIN 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03381">
        <property type="entry name" value="CDC50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015840">
        <property type="entry name" value="DUF284_TM_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="337"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR015840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR21344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="393" mass="44571" checksum="6D15B1C622277999" modified="2009-07-28" version="1">MLWFRKRAAKDSRSSESLTTKSRKPPNTAFRQQRLKAWQPILSPQSVLPLLILISAIFAPIGVALIITANNVQNLSIDYSYCENLANSEAFTTIPTKYTKFHFKRKASVQPTWKLSEDSEGELTCQLQFEVPVNLKKSIYIYYRLTNFFQNHRKYVQSFDIDQLKGKAVDEDSLNDDCNPLKSEGDKVIYPCGLIANSLFNDTFAGVLKGVNDTDTDYQLSNKNIAWHADRKRYKKTTYNASDIVPPPNWAKAFPSGYTDDNIPDLSQWEELQVWMRTAGLPKFYKLALKNESTTLPKGTYRMDIGLNYPVRMFGGSKSFVLTTNSIIGGRNMSLGVVYLIVAGISILFGIIFLVKLIIQPRKLGDHSFLNFDDATESESRPNTSDIPLREIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DC99</accession>
    <name>C5DC99_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">Probable electron transfer flavoprotein subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0B01320g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 7">The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </cofactor>
        <text evidence="7">Binds 1 AMP per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="7">Binds 1 FAD per dimer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterodimer of an alpha and a beta subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the ETF beta-subunit/FixA family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551848.1">
        <property type="nucleotide sequence ID" value="XM_002551802.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DC99"/>
    <dbReference type="FunCoup" id="C5DC99">
        <property type="interactions" value="515"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DC99"/>
    <dbReference type="GeneID" id="8290675"/>
    <dbReference type="KEGG" id="lth:KLTH0B01320g"/>
    <dbReference type="eggNOG" id="KOG3180">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060196_0_1_1"/>
    <dbReference type="InParanoid" id="C5DC99"/>
    <dbReference type="OMA" id="EINQPRI"/>
    <dbReference type="OrthoDB" id="276685at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009063">
        <property type="term" value="P:amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01714">
        <property type="entry name" value="ETF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000011">
        <property type="entry name" value="Electron transfer flavoprotein subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000049">
        <property type="entry name" value="ET-Flavoprotein_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014730">
        <property type="entry name" value="ETF_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012255">
        <property type="entry name" value="ETF_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033948">
        <property type="entry name" value="ETF_beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21294">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN BETA-SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21294:SF8">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01012">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000090">
        <property type="entry name" value="Beta-ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00893">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01065">
        <property type="entry name" value="ETF_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Electron transfer flavoprotein alpha/beta-subunit N-terminal" evidence="8">
        <location>
            <begin position="27"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00070315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR21410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="258" mass="28304" checksum="6489B51E2FEC4093" modified="2009-07-28" version="1">MSKSLRILVPVKRVIDYAIKPRINKAGTGVEKTGVKFSMNPFDDIAVEEAVRIREKHKKLVENIHAVSIGPLKAQDILRTALAKGADTCSHVDVKEAELEPLAVAKILKEMVAKQNANLVILGKQSIDDDCNNTGQMLAGLLNWPQATFAAKVEIDEANMTARVTREIDGGEETVSAPLPLVVTTDLRLNQPRYATLPNLMKAKKKPLEKLSLSDFSKVDAEPRLKVMKVEEPPTRSAGIKVGSVDELIEKLKEVKAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCB1</accession>
    <name>C5DCB1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">dihydroxy-acid dehydratase</fullName>
            <ecNumber evidence="14">4.2.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ordered locus">KLTH0B01606g</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(2R)-2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:24809"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:49072"/>
            <dbReference type="EC" id="4.2.1.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:24810"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>(2R,3R)-2,3-dihydroxy-3-methylpentanoate = (S)-3-methyl-2-oxopentanoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:27694"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:35146"/>
            <dbReference type="ChEBI" id="CHEBI:49258"/>
            <dbReference type="EC" id="4.2.1.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:27695"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="13">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="12">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IlvD/Edd family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.9" evidence="14"/>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551860.1">
        <property type="nucleotide sequence ID" value="XM_002551814.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCB1"/>
    <dbReference type="FunCoup" id="C5DCB1">
        <property type="interactions" value="496"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCB1"/>
    <dbReference type="GeneID" id="8290688"/>
    <dbReference type="KEGG" id="lth:KLTH0B01606g"/>
    <dbReference type="eggNOG" id="KOG2448">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014271_4_1_1"/>
    <dbReference type="InParanoid" id="C5DCB1"/>
    <dbReference type="OMA" id="STQGRNM"/>
    <dbReference type="OrthoDB" id="3851628at2759"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00057"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00061"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004160">
        <property type="term" value="F:dihydroxy-acid dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.80:FF:000001">
        <property type="entry name" value="Dihydroxy-acid dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.80">
        <property type="entry name" value="IlvD/EDD C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00012">
        <property type="entry name" value="IlvD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050165">
        <property type="entry name" value="DHAD_IlvD/Edd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042096">
        <property type="entry name" value="Dihydro-acid_dehy_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004404">
        <property type="entry name" value="DihydroxyA_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020558">
        <property type="entry name" value="DiOHA_6PGluconate_deHydtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056740">
        <property type="entry name" value="ILV_EDD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000581">
        <property type="entry name" value="ILV_EDD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037237">
        <property type="entry name" value="IlvD/EDD_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00110">
        <property type="entry name" value="ilvD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002068">
        <property type="entry name" value="PRK00911.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21000">
        <property type="entry name" value="DIHYDROXY-ACID DEHYDRATASE DAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21000:SF5">
        <property type="entry name" value="DIHYDROXY-ACID DEHYDRATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24877">
        <property type="entry name" value="ILV_EDD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00920">
        <property type="entry name" value="ILVD_EDD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143975">
        <property type="entry name" value="IlvD/EDD N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52016">
        <property type="entry name" value="LeuD/IlvD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00886">
        <property type="entry name" value="ILVD_EDD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00887">
        <property type="entry name" value="ILVD_EDD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dihydroxy-acid/6-phosphogluconate dehydratase N-terminal" evidence="17">
        <location>
            <begin position="58"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="Dihydroxy-acid/6-phosphogluconate dehydratase C-terminal" evidence="18">
        <location>
            <begin position="393"/>
            <end position="584"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00052865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF24877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAR21422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="590" mass="63453" checksum="A8E39494A64EA601" modified="2009-07-28" version="1">MGFLGRLSSTETRRCFSNTRNVYKKLNKYSYIITEPKDQGASQAMLFATGFKQEDFNKPQVGVGSCWWSGNPCNMHLLDFNHRITASVEKAGLKGMQFNTIGVSDGISMGTKGMRYSLQSREIIADSFETIMMAQHYDANIAIPSCDKNMPGVLMAMGRHNRPSIMVYGGTILPGHPSCGSQNIPKNIDVVSAFQSYGQYLSKSFTENERQDVVKNACPGPGSCGGMYTANTMASVAEVLGITLPNSSSHPAVSKEKLAECDSIGEAIKKTMELGITPRDIMTKEAFLNAITYVVATGGSTNAVLHMVAVAQSAGVKITPDDFQRISDKTPLLGDFKPSGKYVMADLIKVGGTQSVIKFLYEEGFLNGNTMTVTGETLEQRAAKAAPLPEGQDIIKPISQPIKPQGHLQILYGSLAPGGAVGKITGKEGTYFKGKARVFDEEAGFIHALERGEIKKGEKTVVVIRYEGPKGGPGMPEMLKPSSALMGYGLGKDVALLTDGRFSGGSHGFLIGHIVPEAAEGGPIALVKDGDEIIIDSNNNKIDVCVSEEELAQRKTQWQEPAPRYARGTLSKYRKLVSNASLGCVTDGDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCC0</accession>
    <name>C5DCC0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">KLTH0B01804p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0B01804g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551869.1">
        <property type="nucleotide sequence ID" value="XM_002551823.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCC0"/>
    <dbReference type="GeneID" id="8290697"/>
    <dbReference type="KEGG" id="lth:KLTH0B01804g"/>
    <dbReference type="eggNOG" id="KOG0758">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015166_16_4_1"/>
    <dbReference type="InParanoid" id="C5DCC0"/>
    <dbReference type="OMA" id="VYRESGW"/>
    <dbReference type="OrthoDB" id="193856at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000064">
        <property type="term" value="F:L-ornithine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990575">
        <property type="term" value="P:mitochondrial L-ornithine transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624:SF57">
        <property type="entry name" value="MITOCHONDRIAL SUBSTRATE CARRIER FAMILY PROTEIN L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="16"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="114"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="210"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR21431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="302" mass="33846" checksum="A366B8337467F684" modified="2009-07-28" version="1">MSARTDNNVVVPNEAYSRIMGFVSGMFSGLAKNAVGHPFDTVKVRLQTSQDTGRFKGPLDCVYQTMRQQGVRGFYLGFTPPLVGWILMDSVMLGCLHNYRMLLKKYVYQHEEKLPLSGCILSGVMAGWSVSFIAAPVELAKAKLQVQYDAKTTKYRGPIDVIKKVYVADGIRGLYKGLISTLIFRTHFIYWWGSYELLTRWFKANTNLSDTAINFWAGGFSASFGFWTTAYPSDVIKQVVLCSDKYDGSFKSWKMAASDIWRSKGFKGFFKGFVPSFLRSFPANAAALASFEFVLRVSGAKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCH3</accession>
    <name>C5DCH3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histone acetyltransferase</fullName>
            <ecNumber evidence="2">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0B03080g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-lysyl-[histone] + acetyl-CoA = N(6)-acetyl-L-lysyl-[histone] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21992"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:21993"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="2"/>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551922.1">
        <property type="nucleotide sequence ID" value="XM_002551876.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCH3"/>
    <dbReference type="FunCoup" id="C5DCH3">
        <property type="interactions" value="46"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCH3"/>
    <dbReference type="GeneID" id="8290755"/>
    <dbReference type="KEGG" id="lth:KLTH0B03080g"/>
    <dbReference type="eggNOG" id="KOG4534">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050421_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCH3"/>
    <dbReference type="OMA" id="FLEHLIV"/>
    <dbReference type="OrthoDB" id="3361892at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032931">
        <property type="term" value="F:histone H3K56 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051236">
        <property type="entry name" value="HAT_RTT109-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013178">
        <property type="entry name" value="Histone_AcTrfase_Rtt109/CBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016849">
        <property type="entry name" value="Rtt109"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31571">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31571:SF2">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE RTT109"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08214">
        <property type="entry name" value="HAT_KAT11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01250">
        <property type="entry name" value="KAT11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51728">
        <property type="entry name" value="RTT109_HAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="393"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR21484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="427" mass="48507" checksum="B5D325A1ED415D52" modified="2009-07-28" version="1">MSSLENLLSRVLPENKKFELLHLQSPPKESHSVFVQQRSDCTQTVVKVQHFFGLGADEKIFYSLELFVYLTIESSKAIEKLIFVSKADTNGYCDEKISVKDITTAILDYIVTLDPSHYLSKEIKVSGTSPGCGPSITRCTTTKRALKILKARHDSKKGDNCPNRGFFRNIEGKDPDWTCKICLFTRSEPQYLFSESSRNPKKHILSGEQLLRWWLSVIDKILVRHFDSGTKATLQIPGEEESRTLRYLSNNKAPFWRVGDIFSGKSSELAIFAIPVFPDDPKGRFLEQVVEENRAKKVRLSDFWVELQVQQEFRLGCTVSVIGVDGRIPKKCFGPHNNEVIVMPSKKVFSIVKSYIVGEQYDTSEGAYEAYANLRDFLHLRMQRSLLEIRGCRKSSEPGSKGTGSDRNAPVRPLNTLTVRRKVKKSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCM6</accession>
    <name>C5DCM6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0B04334p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0B04334g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the BUB2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551975.1">
        <property type="nucleotide sequence ID" value="XM_002551929.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCM6"/>
    <dbReference type="FunCoup" id="C5DCM6">
        <property type="interactions" value="259"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCM6"/>
    <dbReference type="GeneID" id="8290812"/>
    <dbReference type="KEGG" id="lth:KLTH0B04334g"/>
    <dbReference type="eggNOG" id="KOG2058">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029367_2_0_1"/>
    <dbReference type="InParanoid" id="C5DCM6"/>
    <dbReference type="OMA" id="CHKSEPQ"/>
    <dbReference type="OrthoDB" id="10263206at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990334">
        <property type="term" value="C:Bfa1-Bub2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044732">
        <property type="term" value="C:mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031030">
        <property type="term" value="P:negative regulation of septation initiation signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000035">
        <property type="entry name" value="Cell cycle arrest protein BUB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000026">
        <property type="entry name" value="Mitotic check point protein (Bub2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957:SF263">
        <property type="entry name" value="MITOTIC CHECK POINT PROTEIN BUB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="223"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Rab-GAP TBC" evidence="7">
        <location>
            <begin position="37"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00061049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR21537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="302" mass="34010" checksum="05F87B22D8F60FFA" modified="2009-07-28" version="1">MSSLEKFLGQQPLIVHSSLAQLRYLVLSEGIPLSKEKSAARLRCNAWSVLARCSMDGATSDYVSLVRRGPPPHPIYSKIKNDTFRTLNTDPQFIACVSEDAITRCLSSFAWSVLDEEDEEFHLSTYVQGMNVLLAPILYTCPSEPMAFRLFRSLCQDHIKTYLNQSLTGVHNGAKLLDMCLKVIDPKLSKFLADNLLTAEIYGIPSILTLSSCTKPLDQVCKLWDFMFAYGFHMNILFIVAQLVVIRSRVLKSSAPMNLLRMFPEFDAEEIIRLGVGFVAKLPSNLYDLLVQHLEDPNLVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCT9</accession>
    <name>C5DCT9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0B05764p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0B05764g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552038.1">
        <property type="nucleotide sequence ID" value="XM_002551992.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCT9"/>
    <dbReference type="FunCoup" id="C5DCT9">
        <property type="interactions" value="90"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCT9"/>
    <dbReference type="GeneID" id="8290877"/>
    <dbReference type="KEGG" id="lth:KLTH0B05764g"/>
    <dbReference type="eggNOG" id="KOG3485">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_138804_4_1_1"/>
    <dbReference type="InParanoid" id="C5DCT9"/>
    <dbReference type="OMA" id="NTTIEEW"/>
    <dbReference type="OrthoDB" id="274726at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009846">
        <property type="entry name" value="SF3b5/RDS3-10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20978">
        <property type="entry name" value="SPLICING FACTOR 3B SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20978:SF0">
        <property type="entry name" value="SPLICING FACTOR 3B SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07189">
        <property type="entry name" value="SF3b10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR21600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="96" mass="11043" checksum="FB3970DE5DD139A7" modified="2009-07-28" version="1">MSDKHRQKQLFQAMKQKHLGLGTEATTTEEWMTHVHRDTYYSLASHSSMLEYLALSQGDSSKRVTELRLIEKMCGGLSSKRARGENRAEIPFKQTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCY5</accession>
    <name>C5DCY5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0B06820p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0B06820g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the APC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552084.1">
        <property type="nucleotide sequence ID" value="XM_002552038.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DCY5">
        <property type="interactions" value="296"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCY5"/>
    <dbReference type="GeneID" id="8290923"/>
    <dbReference type="KEGG" id="lth:KLTH0B06820g"/>
    <dbReference type="eggNOG" id="KOG1858">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000746_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCY5"/>
    <dbReference type="OMA" id="LEEWHVY"/>
    <dbReference type="OrthoDB" id="26401at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007091">
        <property type="term" value="P:metaphase/anaphase transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070979">
        <property type="term" value="P:protein K11-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000435">
        <property type="entry name" value="Ubiquitin ligase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024990">
        <property type="entry name" value="Apc1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048971">
        <property type="entry name" value="Apc1_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049255">
        <property type="entry name" value="Apc1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12827:SF3">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12827">
        <property type="entry name" value="MEIOTIC CHECKPOINT REGULATOR TSG24 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12859">
        <property type="entry name" value="ANAPC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21282">
        <property type="entry name" value="APC1_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0498">Mitosis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Anaphase-promoting complex subunit 1 N-terminal" evidence="7">
        <location>
            <begin position="54"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Anaphase-promoting complex subunit 1 beta-sandwich" evidence="8">
        <location>
            <begin position="1534"/>
            <end position="1592"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="186"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="235"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="282"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1385"/>
            <end position="1450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="289"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="1403"/>
            <end position="1446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF21282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR21646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1708" mass="191580" checksum="10B56253AFB552E8" modified="2009-07-28" version="1">MPKIPSVFLQNSKHGEVWVFDTRIVEWRLGGKCLRKLEFGGEKVLNAGFVDFESVKECLVVVLEDRAHVYYVGKGDFSIVSFPFSVSKAFFHVNGVLLERKLEEENAVGSKSPFAATKHRFITLSDPLNLFGSLVISSNLPEQQDSKATILHFPRAWDDHVSVLHDCNAKTLYFYNCKILAPARASGSSSSSKSHNHESAPYPANSRKKSYASLAGDAATYGKTSASNKRMLSNEHGSLASSSSSVPHATNAHPNNTATLSTELTNNLRKISIMNRRSASATMGIDLDQPSQTTGPSQSHPGVDYMKRSASATLDRMGSGSALDITPSNSSQFSQELFDHAVLTKDVVLTRISQTPLSIDISPNTHVESCRFEDKEAIILHDPDSQWGKIWIFDLNSSMTESMKFKAFNNTPIALTKIIDIKWSLIRNYCIHNSKLLPGYLMLLSSSGEEITLYNPFLNLTSNPMLIPSREHERALYFSRENLVITSEKASPVNLAILPQIEAVKHCFTAIRYLCDSFTYNYILLLWQVARSSVGEKQAACADFTALKLALLSLLQLERCPVNIKAHMEHIRQSPIFDARFDGARFLPKIVMGMHLISEEYKLNLLQKQNVEMLEGILYLLTTLMKWPRLWKEFYGANEKEDTSFEYKLSNRHFAHPLDEPPSILKSLYSVSDESTIPITPFITFSRLLEDGSSVDHIITPRTNKILQLFESLDAIKFSQSNLLDIMNSLNIDSYELETYPLGVFAPLKRVLKSVENHVSEVNTGSDFTLIDRPDLQKNQEVLKSVQCDTTQTRKPLQPLVSLGPKGRENSKLQGCQPRSIRTIIDDILCLTENQPSDNLSGTNINEEVGEGQVLKRNASLIFSEDRRFFDVVELLLYNIPHKLAVLINEKSYTRALKKKRLFSQILALRTFTSGIGWGAVAFSTEKPLATQKWPQPKMNLHCIFTDKTTVTVDPKECDPSLLSWGEFHGGVSSGLRISRKSTGITGSWITFTKPPELNAQHGGFLLGLGLNGHLKNLEEWHVYNYLSPKQTHTSIGLMLGMSASLRGTMDLKLTKVLSVHIVALLPHGSSDLNVNYRVQTAGLIGVGLLYQNSQHRRMSDMLFAEVSSFVSIDEEAVPDEGYRLAAGIALGLVNIGAGAKAAEAQFKKMAHAETYTSDEDDEDSEFAEFRANNGNLDPKIINGLMNIVNSVHDTEESWMPANSQIGAVVALMLIYLKTNNRTVAEKLAPSSEEINLKARPYTRPELYMYREWAHHMILWDHIASNTEWLFKGINIACALQVNTDFLPIYYCIAGRALALGLKFASSGNSEARDNILALVDKLLPLYQCPIDARLDFQLTIKAINVLLNVLLVSVSMIMSGTGDLAVFRRVKFLHETVSGKNSDLFRTPLRKRQQKRNHTEDEMSVDLTEEPEDGVQAEGNDLGEELEEEEANSTINEELESDDASDESRRYKDEENHFGKFMATSLSLGFLFLGSGQYAFKVSKLENLAYLIMSVLPTYMCPYYLQETKHFWSMAVEYRSLLVCDSRTGASLNKVPVDVTFKGSKSSNTFSTLKMVSPCLLPDVRLIRSIKILSPDYYPVSLDFRSDEDRINFFNNGCVLYVPPREDDGNGWQQNQEAADKSCEVRQILKKKVMSLSSPSASTSKSKCTDDILKHLGLANQESLEIENEVLKLKENQDPLLDENLMMSCQNQSKSYHLELWRRRHKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>C5DD06</accession>
    <name>C5DD06_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0B07304p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0B07304g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552105.1">
        <property type="nucleotide sequence ID" value="XM_002552059.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DD06"/>
    <dbReference type="STRING" id="559295.C5DD06"/>
    <dbReference type="GeneID" id="8290945"/>
    <dbReference type="KEGG" id="lth:KLTH0B07304g"/>
    <dbReference type="eggNOG" id="ENOG502RZ9P">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098355_0_0_1"/>
    <dbReference type="InParanoid" id="C5DD06"/>
    <dbReference type="OMA" id="WGRFDHT"/>
    <dbReference type="OrthoDB" id="4094978at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002948672" evidence="2">
        <location>
            <begin position="21"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR21667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="196" mass="21100" checksum="15A35B3227F77DC8" modified="2009-07-28" version="1">MKVTSLTVGTIAAVSAVASASRGQKQHGDGKTTTITTTTGTHKWGKFNHTSRTHAPKETGTHKWGKFNHTSRTHAPKETGTHKWGKFNHTSRTHAPKETGTHKWGKFNHTSRTHAPKETGTHKWGKFNHTSRTHAPKETGTHKWGRFNKTHNPTSTVIAKNVAANAAAPAAGFDAAHKVLGMTVGSAALVGALMLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>C5DD24</accession>
    <name>C5DD24_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Serine/threonine-protein kinase RIO2</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Serine/threonine-protein kinase rio2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">KLTH0B07722g</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552123.1">
        <property type="nucleotide sequence ID" value="XM_002552077.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DD24"/>
    <dbReference type="FunCoup" id="C5DD24">
        <property type="interactions" value="1349"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DD24"/>
    <dbReference type="GeneID" id="8290963"/>
    <dbReference type="KEGG" id="lth:KLTH0B07722g"/>
    <dbReference type="eggNOG" id="KOG2268">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018693_0_0_1"/>
    <dbReference type="InParanoid" id="C5DD24"/>
    <dbReference type="OMA" id="GYTNFRE"/>
    <dbReference type="OrthoDB" id="10258631at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05144">
        <property type="entry name" value="RIO2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000053">
        <property type="entry name" value="Serine/threonine-protein kinase RIO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000566">
        <property type="entry name" value="Serine/threonine-protein kinase rio2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000052">
        <property type="entry name" value="Serine/threonine-protein kinase RIO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030484">
        <property type="entry name" value="Rio2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015285">
        <property type="entry name" value="RIO2_wHTH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018934">
        <property type="entry name" value="RIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000687">
        <property type="entry name" value="RIO_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45852">
        <property type="entry name" value="SER/THR-PROTEIN KINASE RIO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45852:SF1">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE RIO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01163">
        <property type="entry name" value="RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09202">
        <property type="entry name" value="Rio2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00090">
        <property type="entry name" value="RIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RIO kinase" evidence="16">
        <location>
            <begin position="66"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="346"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="352"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="377"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00068353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00068837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAR21685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="428" mass="49441" checksum="55C6D5579C26AF38" modified="2009-07-28" version="1">MKLDISNMRYMTAEDFRVLQAVEQGSRNHEVVPTQMIHQISGMRSQSGTNRSIGDLAKLKLVSRMRNIKYDGFRLTYNGFDYLGLRAMLSRDTIYSVGNVIGVGKESDVYRVSAKDGSARVLKIHRLGRTSFTTVKNNRDYLKRNQSGSWMFLSRLAANKEYQFMSLLHSKGFTVPEPFDNSRHMILMELIDGYPMRNMRLHKNLPKLYSDLMLFIVKLANHGLIHCDFNEFNIMIKNSVTGPEDNGFIVIDFPQCISIQHQDAEFFFKRDVDCIRRFFKKKLKFEPRVDESMLDVDGFGDGYKYPYPVFERDVKRCDNLDELVQASGFSKKHPGDRRWEEAAEQMRKEVSEQDVVEGDPNDLLEAEESSEGYSEGYSDDEYYSSEEEEESEEGEDSLEEENERIVEALSSGVANLKMDKLGNYVLED</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5DD43</accession>
    <name>C5DD43_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0B08140p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0B08140g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the amino acid-polyamine-organocation (APC) superfamily. Amino acid/choline transporter (ACT) (TC 2.A.3.4) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552142.1">
        <property type="nucleotide sequence ID" value="XM_002552096.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DD43"/>
    <dbReference type="FunCoup" id="C5DD43">
        <property type="interactions" value="211"/>
    </dbReference>
    <dbReference type="GeneID" id="8290982"/>
    <dbReference type="KEGG" id="lth:KLTH0B08140g"/>
    <dbReference type="eggNOG" id="KOG1289">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004495_0_3_1"/>
    <dbReference type="InParanoid" id="C5DD43"/>
    <dbReference type="OMA" id="INSDYGQ"/>
    <dbReference type="OrthoDB" id="4476201at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015101">
        <property type="term" value="F:organic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000046">
        <property type="entry name" value="Amino-acid permease, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649">
        <property type="entry name" value="AMINO-ACID PERMEASE BAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649:SF6">
        <property type="entry name" value="GABA-SPECIFIC PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="72"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="107"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="149"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="197"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="226"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="272"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="305"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="360"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="415"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="440"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="481"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="513"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR21704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="568" mass="61065" checksum="3B14EAC654F897A8" modified="2009-07-28" version="1">MEKAQAVANSTDVSSSLEQAVGDIHQIGSLRTITSKRAGEVNFIDAKRAHGDAELLAEIGYKQELNRHFSTVQVFGVAFSIMGLLPSIASVLAIGMPGGMVSLVWGWFIAGIFILAVGCGMSELASAIPTSGGLYYYTYYYAPQKYKAFFSFVIGYSNSLALVSGLCSITYGLAEEILSIVVVSKDGDFTITNGKTYGVFVAGIAATTVVTSIATVAVSKLQTVSILANVGLIVLFFIVLPIGVANTKGLDFNDGSFIFGKWLNETTWPDGWQFMLAGLQPAIWTIGAFDSCIHMSEEAKNATKAVPLGIISSISVCWILGFLICIVMAACMGPDIDAILGSSFGSPLPQILYNALGKKWTIAIMTLIAACQFLMGCSILTAISRQIWAFARDDGLMFSKYIKVVNKKLSSPLRATWFGAVCALVIGLLCLIGGTASNALFSLGIGGNYLAWMAPNLLRMTTGKDLFRPGAFYMGKTWSPIVNWTAIVFQIFILIMIMFPAESKGITPDAMNYTVVICGGVWIGSMIYFFVYKKKAFNGPKSNLSDDEYVDAVGQDVIDQILSHQKSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>C5DD50</accession>
    <name>C5DD50_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0B08316p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0B08316g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552149.1">
        <property type="nucleotide sequence ID" value="XM_002552103.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DD50"/>
    <dbReference type="FunCoup" id="C5DD50">
        <property type="interactions" value="54"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DD50"/>
    <dbReference type="GeneID" id="8290989"/>
    <dbReference type="KEGG" id="lth:KLTH0B08316g"/>
    <dbReference type="eggNOG" id="ENOG502RYJ3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_299608_0_0_1"/>
    <dbReference type="InParanoid" id="C5DD50"/>
    <dbReference type="OMA" id="MISFESA"/>
    <dbReference type="OrthoDB" id="5376259at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021750">
        <property type="entry name" value="Sid4-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11778">
        <property type="entry name" value="SID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="82"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="531"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="201"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="338"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="462"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="635"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="749"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="27"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="84"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="129"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="148"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="531"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR21711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="950" mass="108474" checksum="90883F4EE26811EC" modified="2009-07-28" version="1">MLGLGSGHIYSMANQSTVSNMSKDEKYDYEDEVSDENEDSGISLTKVRAEQGFSLRSFIDQINAKTKALAIARELDAQRGVTHQDATMSAGSIDSATSASTETQRTQEISSHPTATVRRIPSGARASTAEPNLGSSTPMKEVSRSDNTHNQTINDLLVQSTPINSSKSAPTSRNTQPRDLLKEKFMESHKAQNERSATGNADELQFENVRLREELEFYKKAHDSQEENIQVLQETLQKQDQTAQALREKLQELQDQVEESAQALKVAQDDRRSEFEGHQRVAEELAKCKGQIRLKEDEIVTVTENADKACQDLRTDNAGLHKKAIEIQSALTESELYVQQLKSKVDLMKKHNDDLSQTVHDKIEELNECNTRIDELYKENIAQENSLRSRVKEYDSELSSLRNRNEELQKKLELQDHEFISKSSSYEARISSLKSANEDLEKGIELERTKSSKEAHVLGDTVVTLRQQLADAEKENEKILVRFNMLEKQHLEAENLRDGLEETNQELKSVVTRLEKISSDQLQTIGRLEEEVERKSKKEANSLSSVRKHSKQLEEKVRHLQDVIVAKNSELEMQKQELNQFESYLKSVSLFQIQAGDLIVEQYRLNKYATQMDAAFKERNPDILGFFTQDQLQTTENSAVRLEALDNELHKLEESIKDEWTVKVQQLEEKLKNQELKESTIAKQLAGNNIRIEELNSMVGTLSAEKRDLLHEKEKSMEMQQRLNDQVNSLKGELVDGEHKAFISLSNRIESLGEERLDLQQNLQALESKIKSTETLLNEANALLNQERSEHATSQDEAAQLRKDLARLKGKASRKITFQMASQSRPHLSRKTYDSLMVDAVNDMDMVELQNIIKNIILLLEIPLAKITKKMPLVGIYLRYEKNICLHFANKIHYLMFRETIDIKRYTNVAYGQYLDHHDICHLDHPLESCLDNLYKEVSARLSLSPLTNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDG6</accession>
    <name>C5DDG6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0C00836p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0C00836g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552265.1">
        <property type="nucleotide sequence ID" value="XM_002552219.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DDG6">
        <property type="interactions" value="368"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDG6"/>
    <dbReference type="GeneID" id="8291123"/>
    <dbReference type="KEGG" id="lth:KLTH0C00836g"/>
    <dbReference type="eggNOG" id="KOG1791">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003174_0_0_1"/>
    <dbReference type="InParanoid" id="C5DDG6"/>
    <dbReference type="OMA" id="MTSYWFG"/>
    <dbReference type="OrthoDB" id="72892at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059018">
        <property type="entry name" value="HEAT_URB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039844">
        <property type="entry name" value="URB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032436">
        <property type="entry name" value="URB1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021714">
        <property type="entry name" value="URB1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13500:SF0">
        <property type="entry name" value="NUCLEOLAR PRE-RIBOSOMAL-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13500">
        <property type="entry name" value="NUCLEOLAR PRERIBOSOMAL-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26140">
        <property type="entry name" value="HEAT_URB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16201">
        <property type="entry name" value="NopRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11707">
        <property type="entry name" value="Npa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="URB1 N-terminal" evidence="2">
        <location>
            <begin position="67"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="URB1 central HEAT repeat" evidence="4">
        <location>
            <begin position="579"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="domain" description="URB1 C-terminal" evidence="3">
        <location>
            <begin position="1435"/>
            <end position="1628"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF26140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR21827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1723" mass="195816" checksum="CFF43194A51767FE" modified="2009-07-28" version="1">MSHFRGRFAQTGGAGKPQSQKHGGSSMMDDGVFDKLQSVISEIDLLNEGVSNDYQPVISFFSSKFAAQIIQSWSYHAQVNNHQKFSSCTSRLAKALRVLGSDSSTLIYGSNLVRSILQENTKVLYRGLNNMKPSITNPTLRLLRQIIAFNKGQHVDDFLTCFDMSLPSLPRLLSPTKAELSDLEHTIKNPELSMRFNFIQFWLCLIENATPILRKAVLTENPKIMSAWYKHMTKIDSVKLLIQTLNTLINFVLKERSLKRVTKCKILNESLISKIHYFYSSTNADLVHHTDLFFESYSSVKGDGVTFSDDKIWFTEPVYSSAGAGSGSTGALVVINNKKFYLYNKLLFTMLTFFKPWEDEIQLNRVKKVLENVPELVAPYSHYLGTLGNHDPRMTSYWFGMTLLLGRMMNLPVPPKVREVQTDKHPSSHLVMESIIPCTITKSALTKSLSHNVNLIKQMGCQLLVFSFKKLDIVINLYDEKGWSSAKSELINLFKARIPELSSISSALEAAGQAQNENKVLSASLSVILNYYSKFFPLNFSITLPSDNIFVTIMKSDTFSGLDLVMLDNFLHFQQFNNSHTKWYNPNAGENSIFTSLLRLASSANATNSITNKVKELLENLLNFTVLFNNDELLASPVLVLINSLQVIMRSESCRSSAQESKIWKLLDETVARCMKTPYKYVDMSVPYENCSPFLVALIEQWSFVDKQTPFELVLRWMLIYLRSCYFVREPIESLKPLLEAAPSISSDLQSEYLNFENYDDSLDLLQGQDYLLSNNAPYSFFQHITLVSTSDLDQVARYPTNELDIAGLLTRIQFLLKADTSSMKLQKFCAIVDNLLMKAANYTLSQDDFKQKFASTKYFEKVTIAADIDTENELVCEKSAYVTNSMLEVYLQLDEDVSEVSAYIHHLWGERLPQWSSNPDLMSILIKGLQILSDSQLSEIQLRLLLNNQDLLPLILTEMVKRGTTLPGTRFMELLALKSEKITDVLSEYLTKKLVENPNFESIISLALEDAISYPIIIDCLAYEEGAKKIMGFLDLIKCESLCINVAAAISDKAKDTEASHFVDRALHSACDDLSKLSVLDFEAAMKLFYDCHANASEDLKHNVLGFLISQNEYRFSPWAAKIVELYNVKGDHNISTWMQKSVLYITKTFAERNHLSSKFSEFLQNFKGLLFKVNIWSLGVQSILETQVEVILGGMWVGRAEALEYLNILLLGASNSQVNSNKMLQLLLNNEQSSLNDQKTNSHARFLTSSIMSRLFHLDAAKNATPIVQEKLLLSYNGSVSADDRIIFDVLKEIESRTLSSWTNNVFVWDFQEDFEEDQADDTPLLTKEREGLVLTIKRARINTTIENFACEQPSIPQFASGNVEDTWHRLVAFEEAIELTYQNFERKVYDPLFILLLVANNKELFRNDNSDLESQSQASFFDMRKVVVSGLLELVILSLSERNETKDVALALIYKMIDSLKHGGSFKDSNIFHIFLMKVAYTFHTEKDQEVSPLVWYMISRLSSILTQPSSVLYEKAYKWVLASPSVQPQHLPLYDAVVVTRPTEDGEHYYKYLNWFLEAIQRGIKSEDDIKLLRSRNLLEWMMNLLNSPYINLGQRNTLNLLVYRIQRIETGGPTLITRYAGVSFLEALREGSSEGLVGVSTETLANTKNKLRLKQFLVSQQLDVNYSELGAGYAAVSKSQKRLAEWMEGDVDNVLKRLCTIGNQTSHQTNLNYPFMHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDL4</accession>
    <name>C5DDL4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">U3 small nucleolar ribonucleoprotein protein IMP3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">U3 small nucleolar ribonucleoprotein protein imp3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0C01958g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uS4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552313.1">
        <property type="nucleotide sequence ID" value="XM_002552267.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDL4"/>
    <dbReference type="FunCoup" id="C5DDL4">
        <property type="interactions" value="532"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDL4"/>
    <dbReference type="GeneID" id="8291172"/>
    <dbReference type="KEGG" id="lth:KLTH0C01958g"/>
    <dbReference type="eggNOG" id="KOG4655">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097281_0_0_1"/>
    <dbReference type="InParanoid" id="C5DDL4"/>
    <dbReference type="OMA" id="FRIKHEQ"/>
    <dbReference type="OrthoDB" id="10248812at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034457">
        <property type="term" value="C:Mpp10 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.290.10:FF:000006">
        <property type="entry name" value="U3 small nucleolar ribonucleoprotein IMP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="entry name" value="RNA-binding S4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022801">
        <property type="entry name" value="Ribosomal_uS4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001912">
        <property type="entry name" value="Ribosomal_uS4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831">
        <property type="entry name" value="30S 40S RIBOSOMAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831:SF1">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RIBONUCLEOPROTEIN PROTEIN IMP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00163">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01390">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55174">
        <property type="entry name" value="Alpha-L RNA-binding motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS4 N-terminal" evidence="12">
        <location>
            <begin position="4"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="RNA-binding S4" evidence="11">
        <location>
            <begin position="109"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00069727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00072223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR21875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="183" mass="21703" checksum="2736A5438C32A55F" modified="2009-07-28" version="1">MVRKLKHHEQKLLKKVDFLDWKQDQGHRDTQVMRTYHIQNREDYHKYNKLCGDVRRLSHKLSLLPPSDAFRIKHEQLLLEKLYAMGILTTKSKISDLENKVTVSAICRRRLPVIMHRLKMAETLSDAVKFIEQGHIRVGPNLITDPAYLVTRSMEDYVTWVDSSKIKKTILRYRNQIDDFDFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDN9</accession>
    <name>C5DDN9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">T-complex protein 1 subunit epsilon</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">CCT-epsilon</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0C02530g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552338.1">
        <property type="nucleotide sequence ID" value="XM_002552292.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDN9"/>
    <dbReference type="FunCoup" id="C5DDN9">
        <property type="interactions" value="1435"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDN9"/>
    <dbReference type="GeneID" id="8291198"/>
    <dbReference type="KEGG" id="lth:KLTH0C02530g"/>
    <dbReference type="eggNOG" id="KOG0357">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008891_7_3_1"/>
    <dbReference type="InParanoid" id="C5DDN9"/>
    <dbReference type="OMA" id="SHPQMPH"/>
    <dbReference type="OrthoDB" id="10248520at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03339">
        <property type="entry name" value="TCP1_epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000003">
        <property type="entry name" value="T-complex protein 1 subunit epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012718">
        <property type="entry name" value="Chap_CCT_epsi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02343">
        <property type="entry name" value="chap_CCT_epsi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 10" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR21900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="560" mass="61308" checksum="8F5A6E6340419F54" modified="2009-07-28" version="1">MAQMQPQGMQMPDMSNAIVAQDEMGRPFIIVKDQGTKQRQHGLEAKKSHILAARSVASIIKTSLGPRGLDKILISPDGEITITNDGATILSQMELDNEIAKLLVQLSKSQDDEVGDGTTGVVVLASALLDQALELIEKGIHPIKIANGFDEAAKVAVSHLDSQADDIAHDSASFHDYLFKAAKTSLGSKIVSKDHDKFAQMAVDAVTSVMDLDRKDVDFDLIKLEGRVGGALQDSKLIKGVVLDKDFSHPQMPKTVTAKEGHDGVKLAILTCPFEPPKPKTKHKLDISTVEEYQKLQSYEQDKFKEMIEYVKNAGADVAICQWGFDDEANHLLLQNGLPAVRWVGGQELELIAIATKGRIVPRFQDLSPEKLGTCGRIREVEFGTTKDRMLVIEECSNSKTVTCFIRGSNKMIVDEAKRALHDSLCVVRNLVRDSRVVYGGGAAEVTMSVAVSEEADKQHGIDQYAFRAFSQALDTIPMLLAENSGLDPIETLALLKSTQIKNKTSTTGVDCLANDSNNMKELFVVDPLIGKKQQILLATQLCRMILKIDNVIISGKDEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDX2</accession>
    <name>C5DDX2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0C04488p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0C04488g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552421.1">
        <property type="nucleotide sequence ID" value="XM_002552375.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDX2"/>
    <dbReference type="FunCoup" id="C5DDX2">
        <property type="interactions" value="144"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDX2"/>
    <dbReference type="GeneID" id="8291286"/>
    <dbReference type="KEGG" id="lth:KLTH0C04488g"/>
    <dbReference type="eggNOG" id="KOG1050">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002351_2_0_1"/>
    <dbReference type="InParanoid" id="C5DDX2"/>
    <dbReference type="OMA" id="CHEFIVC"/>
    <dbReference type="OrthoDB" id="755951at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005946">
        <property type="term" value="C:alpha,alpha-trehalose-phosphate synthase complex (UDP-forming)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003825">
        <property type="term" value="F:alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004805">
        <property type="term" value="F:trehalose-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03788">
        <property type="entry name" value="GT20_TPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000099">
        <property type="entry name" value="Alpha,alpha-trehalose phosphate synthase subunit, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000036">
        <property type="entry name" value="Alpha,alpha-trehalose-phosphate synthase subunit Tps2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001830">
        <property type="entry name" value="Glyco_trans_20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003337">
        <property type="entry name" value="Trehalose_PPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788:SF15">
        <property type="entry name" value="TREHALOSE SYNTHASE COMPLEX REGULATORY SUBUNIT TPS3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788">
        <property type="entry name" value="TREHALOSE-6-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00982">
        <property type="entry name" value="Glyco_transf_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02358">
        <property type="entry name" value="Trehalose_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="36"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="202"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="244"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="293"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="55"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="208"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR21983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1105" mass="122400" checksum="4A9551AD33557285" modified="2009-07-28" version="1">MVVIVVSLFLPFQPQFEIDASEGDAAALIESQPVKLPSNSYAGPESGDSLRKNRANSVHSNSPFLGSDLGDSEHANPGVSREADGTPAKGPVTDISSEKFMENLTANAHNTGATGTPVNPIHNHANTSVEEFFSSHSSAAPLANLNQSGASSKNYSATSTPGTEAIFSPVPTHDPTANLLRNVNKSLYQNVVSGGASPVLPAAFEGNTPPQASQPSGTVITPKSRELPDLNSSVVDLPKAKQQQYSLPAMRRVHRKSSNASPPPVEAKEFKAQPHLAKHALVPSALKNEVIEESDSDNFLSDNEGDERSRVPQFGGYSNTARTRSALLRGSKNIFGRVPWKIVASGKGNGGLKNAITTAAAEKTIPEPVKWIGTMGIPTDSVPGNVLKDVSNELSSNYNSVAIISDDITFKGAYKNFCKQILWPTLHYQIPDNPKSKAFEDHSWNYYQDLNQSFADKIVETYNDGDIIWIHDYHLMLVPGMVRQKIPDAKIGFFLHVSFPSSEVFRCFAQRESILNGIVGANYVGFQTEEYARHFLQTANRLLMTDVDTKTLKYRGRVIEVGSAPIGIDAFILESELRSEQVQNWRQLIRGRWAGKKLIIGRDQFDRIRGLRKKLLAYERFLRANPDYVSRVVLIQICLGSDKDPDLEREIMIVVDRINALSPDISVSQPVVFLHQDLEFAQYLALNCEADMFLITTMREGMNLTCHEFIVCSREKNAPLLLSEFTGSASTMKDGAFLINPWDTKQIAQDIKTALDLSPEEKRRRWKELYKSIMRNDSDFWIKHSLSEINTSWDFNQERSTVFNLSLDSLLSSYKSSKKRVFILKLSEPPTARIISILSDLSATNIVYAFSSFSRNMMERLYTRTPNVGLVAENGAFVRLNGHWFNIVEDNSWKDDVIKVIEDKVERLPGSYYKAGESLVRFHAENAEDQDRVSGVVGEAITHINTLFGDRGIHAYLHKNIVFVQQVGLALKAVQFLMGYFNSISSTTNTTPQHSVENSPLLSPMMSQGMANDVTSPGAQSSGSSYFSLGRNVTHVDFLAITGSSSPVIEPLFDYVNDLAKKRSIKTGYSVVYGISSLSTYAKDHVEGLNELFSMLEKLIESHQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDX8</accession>
    <name>C5DDX8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0C04664p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0C04664g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552427.1">
        <property type="nucleotide sequence ID" value="XM_002552381.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDX8"/>
    <dbReference type="FunCoup" id="C5DDX8">
        <property type="interactions" value="329"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDX8"/>
    <dbReference type="GeneID" id="8291294"/>
    <dbReference type="KEGG" id="lth:KLTH0C04664g"/>
    <dbReference type="eggNOG" id="KOG2177">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037664_0_0_1"/>
    <dbReference type="InParanoid" id="C5DDX8"/>
    <dbReference type="OMA" id="NSEMTDY"/>
    <dbReference type="OrthoDB" id="6105938at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15227">
        <property type="entry name" value="zf-C3HC4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="5"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="163"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="163"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="204"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="222"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="259"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR21989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="306" mass="34491" checksum="2C7710812F43FC8F" modified="2009-07-28" version="1">MYVPVMTSCGHNYCYECISNWLVSNNANELTCPQCRSPLKEPPALNVALQNLLNYVVDNYAISSLQTDGTRAESVDQYRDDNFSGRLFKNVFNNTAMAIVDDDDGVARCSNCHWEVEGDVCPHCSARMRNRAVEEEEFPSDEYSEDEIERIQYGANRALGVRDEQGADEHSESDSESDESNFERRNGTLANISGHAQLGRSRRRFDGSQHGSEDLMNSEGAMESEDAEDSDLDSFIVNEEEEDEIGGEQEEPEQVELDGSDHDSDFYEHHDIDGFVSGDSLDNDSEDGHKKRKRRIQVVLDSDEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>C5DE06</accession>
    <name>C5DE06_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pre-mRNA-splicing factor SLT11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0C05280g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in pre-mRNA splicing. Facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome. Binds to RNA.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552455.1">
        <property type="nucleotide sequence ID" value="XM_002552409.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DE06"/>
    <dbReference type="FunCoup" id="C5DE06">
        <property type="interactions" value="181"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DE06"/>
    <dbReference type="GeneID" id="8291322"/>
    <dbReference type="KEGG" id="lth:KLTH0C05280g"/>
    <dbReference type="eggNOG" id="KOG0153">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027112_1_1_1"/>
    <dbReference type="InParanoid" id="C5DE06"/>
    <dbReference type="OMA" id="RNVCQCC"/>
    <dbReference type="OrthoDB" id="10259600at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071006">
        <property type="term" value="C:U2-type catalytic step 1 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071007">
        <property type="term" value="C:U2-type catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036002">
        <property type="term" value="F:pre-mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017070">
        <property type="term" value="F:U6 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039171">
        <property type="entry name" value="Cwc2/Slt11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048995">
        <property type="entry name" value="STL11/RBM22-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14089">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR RBM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14089:SF6">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR RBM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21369">
        <property type="entry name" value="STL11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="STL11/RBM22-like N-terminal" evidence="5">
        <location>
            <begin position="3"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="305"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00019060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF21369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR22017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="338" mass="37075" checksum="374E2571F8D5179C" modified="2009-07-28" version="1">MEPAICKSCLGEQERLTRAVNGAECKICTLPFTVYHFKAHHRINRTVICYNCSKQRNVCQCCLLDLQWQIPVELRDRVLSLIQGSEVATHEAQNEMMKRFIALKDGDSFKMGGASITSSASATADALKQIRDTVEAVEKSGKAADAAGPGRDVPLPKNLADLDITHLLKKLPLKGSLSDGTSFFLYNIDPSIPEWAIVDAVTELVGTASWKGPVSASTIINHTARCGGIRFKTLELAQKFIDQVPTFKTEAGSIKGKLLVQNSRIHVVSWSQFNRAALGTKNAECLKLAASLDKLVQKDLNSPAASNTMLTKKQGKVTKRAPSSKDKKRNRRVKDIEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DE97</accession>
    <name>C5DE97_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">3-deoxy-7-phosphoheptulonate synthase</fullName>
            <ecNumber evidence="2">2.5.1.54</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0C07370g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>D-erythrose 4-phosphate + phosphoenolpyruvate + H2O = 7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14717"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16897"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="2.5.1.54"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I DAHP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.54" evidence="2"/>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552546.1">
        <property type="nucleotide sequence ID" value="XM_002552500.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DE97"/>
    <dbReference type="FunCoup" id="C5DE97">
        <property type="interactions" value="193"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DE97"/>
    <dbReference type="GeneID" id="8291415"/>
    <dbReference type="KEGG" id="lth:KLTH0C07370g"/>
    <dbReference type="eggNOG" id="ENOG502QPSU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030903_0_1_1"/>
    <dbReference type="InParanoid" id="C5DE97"/>
    <dbReference type="OMA" id="DINTGLR"/>
    <dbReference type="OrthoDB" id="4699125at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003849">
        <property type="term" value="F:3-deoxy-7-phosphoheptulonate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000005">
        <property type="entry name" value="Phospho-2-dehydro-3-deoxyheptonate aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006218">
        <property type="entry name" value="DAHP1/KDSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006219">
        <property type="entry name" value="DAHP_synth_1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00034">
        <property type="entry name" value="aroFGH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009395">
        <property type="entry name" value="PRK12755.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21225">
        <property type="entry name" value="PHOSPHO-2-DEHYDRO-3-DEOXYHEPTONATE ALDOLASE DAHP SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21225:SF18">
        <property type="entry name" value="PHOSPHO-2-DEHYDRO-3-DEOXYHEPTONATE ALDOLASE, PHENYLALANINE-INHIBITED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00793">
        <property type="entry name" value="DAHP_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0346">Stress response</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DAHP synthetase I/KDSA" evidence="8">
        <location>
            <begin position="88"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR22108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="410" mass="45507" checksum="D9D3EBED60319CC3" modified="2009-07-28" version="1">MTDLQKKKFHRHIRRDAAVLLLDRVPSSRSLDFPCYPEMFIKNDHAGDRKRLEDWRIKGYDPLTPPDLLQHELPISEKGAKNIVDAREQVCDILNGKDDRLVIVIGPCSIHDPKAAYEYCDRLSQISQKLSKDLLIIMRAYLEKPRTTVGWKGLINDPDIDNSFQINKGLRISREMFTNLVEKMPIAGEMLDTISPQFLSDCFSLGAIGARTTESQLHRELASGLSFPIGFKNGTDGGLQVSIDAMRAAAHAHYFLSVTKPGVTAIVGTEGNSDTFIILRGGKKGTNFDAESVKQAQSELIQAKVVDENSSARRIMIDCSHGNSSKDYRNQPKVAQSIFEQLTDGQNAICGVMIESNLVEGRQDITPEGGREGLKYGCSITDACIGWESTEEVLTLLAKGVQNRRKALSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEB3</accession>
    <name>C5DEB3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0C07788p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0C07788g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552562.1">
        <property type="nucleotide sequence ID" value="XM_002552516.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEB3"/>
    <dbReference type="FunCoup" id="C5DEB3">
        <property type="interactions" value="1417"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEB3"/>
    <dbReference type="GeneID" id="8291434"/>
    <dbReference type="KEGG" id="lth:KLTH0C07788g"/>
    <dbReference type="eggNOG" id="KOG0051">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016706_0_0_1"/>
    <dbReference type="InParanoid" id="C5DEB3"/>
    <dbReference type="OMA" id="HVRRKYH"/>
    <dbReference type="OrthoDB" id="39591at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031981">
        <property type="term" value="C:nuclear lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000387">
        <property type="entry name" value="Replication termination factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051651">
        <property type="entry name" value="DMTF1_DNA-bind_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46380">
        <property type="entry name" value="CYCLIN-D-BINDING MYB-LIKE TRANSCRIPTION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46380:SF2">
        <property type="entry name" value="CYCLIN-D-BINDING MYB-LIKE TRANSCRIPTION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="HTH myb-type" evidence="7">
        <location>
            <begin position="457"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="6">
        <location>
            <begin position="461"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="6">
        <location>
            <begin position="509"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="7">
        <location>
            <begin position="570"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="22"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="315"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="536"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="25"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="59"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="162"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="188"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="316"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="537"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR22124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="690" mass="76560" checksum="20F574F5C7DE046F" modified="2009-07-28" version="1">MSDGRADQESVEEAVFKYVGVDLQGGKHDEGHPHELEAAENKRQAQARLAHVARDARARAQAQVQAQAQAQARAQAQTQSQVRAGAGSSTGGQDQHGQGQDHDQDQHGQGQDANPVQDQDQDPDQDQDQAAGSADMEWFLRHELGASPSDLDMRGAREPQSVAAAAVAAAFAASQDNKRPRNEDDNGSGRNAASNVSGPRQHHGNKRFKVPDARLAGSRVQSKMPHLGAVDPELASLDDPEDVTEHEQLVRKAILDADSIAHHPDFQHYLNTEDDSDDKAQRMSLLSSKGSTVVLGGIHDHGRKIEVLPKVAGRNSVSGVDSHSGSSPGSGSGPGAGLAARSMGRIPSGMTLGAGAGAGAHSEQDISNMIQDAATKASDFISAQNQSTGKSFDAVEEQALEHFVGDYQAIKNLSRRQICERIWSNERRKDDFWTNICKVLPYRTRSSVYKHVRRKYHIFEQRGKWTPEEDQELAQLCMEKEGQWSEIGKTLGRMPEDCRDRWRNYVKCGNNRASNKWSPQEEEHLKQVISELLDSVEPQDDQSYDTDEVHMDGKAKAGKKKKQDSKDTINWTVVSERMGGTRSRIQCRYKWNKLLKKQAMSKIKSISEDDKTWILEKLRDMGFTEDSQVDWDELATLMPGRQWSGTELKLCYEKLRTGVKYYKDRTINQICKELIGARDEGLPLESESKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEF5</accession>
    <name>C5DEF5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA pseudouridine(32) synthase</fullName>
            <ecNumber evidence="3">5.4.99.28</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0C08756g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>uridine(32) in tRNA = pseudouridine(32) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:42544"/>
            <dbReference type="Rhea" id="RHEA-COMP:10107"/>
            <dbReference type="Rhea" id="RHEA-COMP:10108"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.28"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="5.4.99.28" evidence="3"/>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552604.1">
        <property type="nucleotide sequence ID" value="XM_002552558.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEF5"/>
    <dbReference type="FunCoup" id="C5DEF5">
        <property type="interactions" value="536"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEF5"/>
    <dbReference type="GeneID" id="8291478"/>
    <dbReference type="KEGG" id="lth:KLTH0C08756g"/>
    <dbReference type="eggNOG" id="KOG1018">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1919">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016902_12_4_1"/>
    <dbReference type="InParanoid" id="C5DEF5"/>
    <dbReference type="OMA" id="PKGADEM"/>
    <dbReference type="OrthoDB" id="424794at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019239">
        <property type="term" value="F:deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016814">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amidines"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160151">
        <property type="term" value="F:tRNA pseudouridine(32) synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02557">
        <property type="entry name" value="PseudoU_synth_ScRIB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000061">
        <property type="entry name" value="DRAP deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2350.10:FF:000017">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.10">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006224">
        <property type="entry name" value="PsdUridine_synth_RluA-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006225">
        <property type="entry name" value="PsdUridine_synth_RluC/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050188">
        <property type="entry name" value="RluA_PseudoU_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00005">
        <property type="entry name" value="rluA_subfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21600">
        <property type="entry name" value="MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21600:SF40">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE RPUSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18785">
        <property type="entry name" value="Inv-AAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01129">
        <property type="entry name" value="PSI_RLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="7">
        <location>
            <begin position="455"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="27"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="39"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00036184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR606225-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR22166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="610" mass="69128" checksum="EFC8767822F6F190" modified="2009-07-28" version="1">MRWIAQRNSALTKSTYRFMCELPKDSNLTESKKRPVTPPKTTGSSDHSPSATEHLCVKKKKNGGPGELRDSKGFKLKIQESNHLRNKQVDPEYEVQVEGPLRKIVPYDFTYKTFCKLRWRDRPLIDIFVNEFRDREESYYRKTIARGAVFLNENPANLESIVRNGDLISHKIHRHEPPVTSRPIKIVFEDQDLFVIDKPSGIPVHPTGRYRFNTITKILQKQINGSVHPCNRLDRLTSGLMFLAKTPRGADHMANQLKAREVKKEYIARVVGEFPAERISVEKPLKCVEPRLALNGVCNSEEEGAKEAKTVFTRVSFDGETSLVKCQPHTGRTHQIRVHLQYLGHPIANDPLYSNPQIWGPSLGKGQRTDFSEVIEKLSNVGKTVCAESWFFPDSMGELLTGEKCPDCETDLYTDPGPNDLDLWLHAYCYESTEFNDNGTRKWSYKTEFPEWALEPHRKYMEMAINEAKKCGPTTTAFSVGAVLTNSDKVLSTGFSRELPGNTHAEQCALEKYFEQSGNRSVPPGSVLYTTMEPCSFRLSGNKPCVDRIIDASKSIRTVFVGVMEPDTFVKNNTSKTKLSQHEIDYVLIPGYEEDCTKAAKKGHKEDSGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEG5</accession>
    <name>C5DEG5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">KLTH0C08976p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0C08976g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MNN1/MNT family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552614.1">
        <property type="nucleotide sequence ID" value="XM_002552568.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEG5"/>
    <dbReference type="STRING" id="559295.C5DEG5"/>
    <dbReference type="GeneID" id="8291488"/>
    <dbReference type="KEGG" id="lth:KLTH0C08976g"/>
    <dbReference type="eggNOG" id="ENOG502REUR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013298_1_1_1"/>
    <dbReference type="InParanoid" id="C5DEG5"/>
    <dbReference type="OMA" id="DLELANW"/>
    <dbReference type="OrthoDB" id="430354at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046354">
        <property type="term" value="P:mannan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022751">
        <property type="entry name" value="Alpha_mannosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31646">
        <property type="entry name" value="ALPHA-1,2-MANNOSYLTRANSFERASE MNN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31646:SF1">
        <property type="entry name" value="ALPHA-1,2-MANNOSYLTRANSFERASE MNN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11051">
        <property type="entry name" value="Mannosyl_trans3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR22176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="702" mass="79544" checksum="B8629748E0765D76" modified="2009-07-28" version="1">MSFTRIIRDLSVRALAVLDLVGTRFRQSNHVWRRGKGKGISTIVLGVFGLIVLGLIIGVAHKSNEDLDFLDYDGGAPGVDYAYEDLGGDASYEPPADEEVGRKPDDPQVSEATKKAFKEVLDAIQANSPEGQLQVSKGSECNLMDIGVTDTSKFDRLTKESLEKCLQIPEEMTKTLHEAHKKFRAAIGEKLMPMFRKLDTPAFSGDGILIVGGGKYSVFALPAIKAIRENSGFKLKSSIPIEIIIPPSDKADRGFCDNVLPKVDPLGLTKCVFLDEMFDADTLSHIEGYQIKALALLVSRFERVLMLDADNYVVNSINDYFTSSALNDKGLILWPDYWRRLHHPKLYGIVDLGVSPDHISRYSVDDVSPDYMYKKSVTDTPFHDFRDALPDGGTESGQLLVDKKKHLDSIIMSLYYNYNGKSYYYPILGQGFAGEGDKDTFALASRVMHGPGSFYQVKTPVDTLGHWADAKDEIRLLEEEYENVEKKFRGCAMLQHDYIEDSKFSSLAREVLGNTIRNNEEKFCDEWAAKNKGKFSAEEKARREECKKSKEVQDALHEKMRASYKLDDYLAFFSFTGVSFVHSHLPKYDPWEWYENGDMMFDGKKAKKNHKNDPDYVPAHSGNYRMYDSKFNQISSYDLELANWSAFKSYLCDLEGGYKNFGYLSNKIDSTKSPKQSNKDMCKYIEERVEYLKSTSWNDYDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEH9</accession>
    <name>C5DEH9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">KLTH0C09372p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0C09372g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>myo-inositol(out) + H(+)(out) = myo-inositol(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:60364"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552628.1">
        <property type="nucleotide sequence ID" value="XM_002552582.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEH9"/>
    <dbReference type="FunCoup" id="C5DEH9">
        <property type="interactions" value="1312"/>
    </dbReference>
    <dbReference type="GeneID" id="8291506"/>
    <dbReference type="KEGG" id="lth:KLTH0C09372g"/>
    <dbReference type="eggNOG" id="KOG0254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_30_5_1"/>
    <dbReference type="InParanoid" id="C5DEH9"/>
    <dbReference type="OMA" id="ETGWRWM"/>
    <dbReference type="OrthoDB" id="6339427at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005366">
        <property type="term" value="F:myo-inositol:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904679">
        <property type="term" value="P:myo-inositol import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17360">
        <property type="entry name" value="MFS_HMIT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000073">
        <property type="entry name" value="MFS myo-inositol transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050814">
        <property type="entry name" value="Myo-inositol_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020:SF12">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="144"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="173"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="200"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="230"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="258"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="351"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="387"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="415"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="458"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="502"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="528"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="11">
        <location>
            <begin position="103"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="578"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="8"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="24"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="592"/>
            <end position="608"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR22190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="608" mass="65635" checksum="176A96AB50ED10B5" modified="2009-07-28" version="1">MTLRKPESGVGSASGAESALAGRAQDRAQGHGHGFHEIDASKDSNDDSAQSITVIEPDVTNGKLDADGALLDDRIQIRPVNDEDDTSVMITFNQKVSPFIIVLTFVASISGFMFGYDTGYISSALVSIGTDLDHRALTYGDKEIITAATSLGALISSIFAGTSADIWGRKPCLMFSNLLFTVGAVLQISARSFWQMAVGRLIMGFGVGIGSLISPLFIGEIAPKNIRGRLVVINSLCLTGGQLVAYGCGAGLDKVHNGWRILVGLSLIPTCIQFVCFWFLPDTPRYYVMKGRLDKAAEVLGKSYVDAPSELIHQKIQELNALNRTIPGDTIPKKVWNTIKEIHTVPSNFRALILGCALQGIQQFCGWNSLMYFSGTIFETVGFSDSAAVSIVVAGTNFVFTLVAFFAIDRIGRRCILLIGLPGMCGSLIVCAVAFHFLGVHFEGGGQATIEHQGFSAWGIIVILSIIFYAAFYALGIGTVPWQQSELFPQAVRGIGTSYSTATNWGGSLVIASTFLTMLQNITPTGTFALFAGLSLVSFFFCYFCYPELSGLELEEVQMILKDGFNIKASKTLAKKRKQQLAAQKNSSGNGDLHDYHKSKPSDELMEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEJ6</accession>
    <name>C5DEJ6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ubiquinone biosynthesis O-methyltransferase, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">3,4-dihydroxy-5-hexaprenylbenzoate methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">3-demethylubiquinol 3-O-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">3-demethylubiquinone 3-O-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">3-demethylubiquinone-6 3-O-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Hexaprenyldihydroxybenzoate methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Polyprenyldihydroxybenzoate methyltransferase</fullName>
            <shortName evidence="5">DHHB methyltransferase</shortName>
            <shortName evidence="5">DHHB-MT</shortName>
            <shortName evidence="5">DHHB-MTase</shortName>
            <ecNumber evidence="5">2.1.1.-</ecNumber>
            <ecNumber evidence="5">2.1.1.114</ecNumber>
            <ecNumber evidence="5">2.1.1.64</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">COQ3</name>
        <name evidence="6" type="ordered locus">KLTH0C09812g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">O-methyltransferase required for two non-consecutive steps during ubiquinone biosynthesis. Catalyzes the 2 O-methylation of 3,4-dihydroxy-5-(all-trans-polyprenyl)benzoic acid into 4-hydroxy-3-methoxy-5-(all-trans-polyprenyl)benzoic acid. Also catalyzes the last step of ubiquinone biosynthesis by mediating methylation of 3-demethylubiquinone into ubiquinone. Also able to mediate the methylation of 3-demethylubiquinol into ubiquinol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 3,4-dihydroxy-5-(all-trans-polyprenyl)benzoate + S-adenosyl-L-methionine = a 4-hydroxy-3-methoxy-5-(all-trans-polyprenyl)benzoate + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:44452"/>
            <dbReference type="Rhea" id="RHEA-COMP:10930"/>
            <dbReference type="Rhea" id="RHEA-COMP:10931"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64694"/>
            <dbReference type="ChEBI" id="CHEBI:84443"/>
            <dbReference type="EC" id="2.1.1.114"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 3-demethylubiquinol + S-adenosyl-L-methionine = a ubiquinol + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:44380"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="Rhea" id="RHEA-COMP:10914"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:84422"/>
            <dbReference type="EC" id="2.1.1.64"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 3-demethylubiquinone + S-adenosyl-L-methionine = a ubiquinone + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:81215"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:19654"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:231825"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of a multi-subunit COQ enzyme complex, composed of at least COQ3, COQ4, COQ5, COQ6, COQ7 and COQ9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Mitochondrion inner membrane</location>
            <topology evidence="5">Peripheral membrane protein</topology>
            <orientation evidence="5">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the class I-like SAM-binding methyltransferase superfamily. UbiG/COQ3 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="5"/>
    <dbReference type="EC" id="2.1.1.114" evidence="5"/>
    <dbReference type="EC" id="2.1.1.64" evidence="5"/>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552645.1">
        <property type="nucleotide sequence ID" value="XM_002552599.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEJ6"/>
    <dbReference type="FunCoup" id="C5DEJ6">
        <property type="interactions" value="362"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEJ6"/>
    <dbReference type="GeneID" id="8291525"/>
    <dbReference type="KEGG" id="lth:KLTH0C09812g"/>
    <dbReference type="eggNOG" id="KOG1270">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042432_3_0_1"/>
    <dbReference type="InParanoid" id="C5DEJ6"/>
    <dbReference type="OMA" id="LASRWWD"/>
    <dbReference type="OrthoDB" id="3265906at2759"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031314">
        <property type="term" value="C:extrinsic component of mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061542">
        <property type="term" value="F:3-demethylubiquinol 3-O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120537">
        <property type="term" value="F:3-demethylubiquinone 3-O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010420">
        <property type="term" value="F:polyprenyldihydroxybenzoate methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00472">
        <property type="entry name" value="UbiG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010233">
        <property type="entry name" value="UbiG_MeTrfase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01983">
        <property type="entry name" value="UbiG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43464">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43464:SF19">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS O-METHYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13489">
        <property type="entry name" value="Methyltransf_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 5" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="binding site" evidence="5">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR22207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="306" mass="34726" checksum="25D43B82A38AA8E4" modified="2009-07-28" version="1">MIRVLKRAYTKFNPEFLKPVSSGATTSQGGSTAASQDEVLHFTELAPSWWDVSGPQRILHKMNLARMDFIQKTLREQFKAGPDTYVPGFNYKEHLPARVSTAIEQELDQDVRSQLQKHKYRVLDIGCGGGILTESLARMPFVSKVDGVDLTPECIEVARGHAEKDPLVKNKLNYQLVPLEDVTENYDLVTCMEMLEHVDQPSEILRHAWNRLNRGGVLFLSTINRDPISWFTTIFVAERVIKVVPSGTHHLSKYINSEEIEQWFKDECAGQYQVLDCKGTMYVPLKGWAIHECSGIGNYIMAVKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEK1</accession>
    <name>C5DEK1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">tRNA (guanine(9)-N1)-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.221</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">tRNA methyltransferase 10</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">tRNA(m1G9)-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0C09922g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>guanosine(9) in tRNA + S-adenosyl-L-methionine = N(1)-methylguanosine(9) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43156"/>
            <dbReference type="Rhea" id="RHEA-COMP:10367"/>
            <dbReference type="Rhea" id="RHEA-COMP:10368"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="EC" id="2.1.1.221"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.1.1.221" evidence="1"/>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002552604.1"/>
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    <dbReference type="AlphaFoldDB" id="C5DEK1"/>
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        <property type="interactions" value="825"/>
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    <dbReference type="STRING" id="559295.C5DEK1"/>
    <dbReference type="GeneID" id="8291530"/>
    <dbReference type="KEGG" id="lth:KLTH0C09922g"/>
    <dbReference type="eggNOG" id="KOG2967">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034384_1_0_1"/>
    <dbReference type="InParanoid" id="C5DEK1"/>
    <dbReference type="OMA" id="FKKNDGW"/>
    <dbReference type="OrthoDB" id="278300at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052905">
        <property type="term" value="F:tRNA (guanosine(9)-N1)-methyltransferase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="F:tRNA binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:tRNA N1-guanine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18089">
        <property type="entry name" value="SPOUT_Trm10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028564">
        <property type="entry name" value="MT_TRM10-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038459">
        <property type="entry name" value="MT_TRM10-typ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016653">
        <property type="entry name" value="TRM10/TRM10A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007356">
        <property type="entry name" value="tRNA_m1G_MeTrfase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13563">
        <property type="entry name" value="TRNA (GUANINE-9-) METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13563:SF13">
        <property type="entry name" value="TRNA METHYLTRANSFERASE 10 HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016323">
        <property type="entry name" value="tRNA_m1G_mtfrase_met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51675">
        <property type="entry name" value="SAM_MT_TRM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent MTase TRM10-type" evidence="12">
        <location>
            <begin position="81"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="50"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="270"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="56"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="274"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="201"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR016323-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR016323-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR22212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="302" mass="35060" checksum="58C8EEE48E024F8E" modified="2009-07-28" version="1">MSDSKPEKPITMHPHPLPPVPEGMSKSQWKKVWKKKRFEETKDEFAKIRREKRQKAKETRRAKIQEYLDRGEEVPEDLRRKPRKNQDQKDSGINIILDCAFDDLMNDKEVVSLSTQITRAYSHNKRENHFAKVKVTSFNKRLRTRFEEGLKDAHHDEWKNFEFTEDPTLPTENSVYLTADTDETLEKLEPGTNYIVGGIVDKNRHKLLCYNKARELGIPTKKLPLAEFIKLTGREVLTCTHVIHLMLRYFDNLDWKEAFETVLPQRKLEEAEAAAEAAAKAQSPQAESSEPSSATSEEEPQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEP8</accession>
    <name>C5DEP8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0C11044p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0C11044g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ADIPOR family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552697.1">
        <property type="nucleotide sequence ID" value="XM_002552651.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEP8"/>
    <dbReference type="FunCoup" id="C5DEP8">
        <property type="interactions" value="339"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEP8"/>
    <dbReference type="GeneID" id="8291581"/>
    <dbReference type="KEGG" id="lth:KLTH0C11044g"/>
    <dbReference type="eggNOG" id="KOG0748">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023075_2_0_1"/>
    <dbReference type="InParanoid" id="C5DEP8"/>
    <dbReference type="OMA" id="IGNACDY"/>
    <dbReference type="OrthoDB" id="529367at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006882">
        <property type="term" value="P:intracellular zinc ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004254">
        <property type="entry name" value="AdipoR/HlyIII-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855:SF52">
        <property type="entry name" value="ADIPONECTIN RECEPTOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855">
        <property type="entry name" value="ADIPOR/PROGESTIN RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03006">
        <property type="entry name" value="HlyIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="83"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="117"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="181"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="215"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="249"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="286"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR604254-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR22259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="315" mass="36274" checksum="FC9BF496EF7002C7" modified="2009-07-28" version="1">MEQGGLTQRKPVEVELHHRMVEENSSLLKDSNLKQRTKRRLYTWDEIPEWQRDNEHIVGGYILETKSTWECLKSLLYLHNESVNIYTHLLPGWCFLLVAVFNQVVIEKFETTRLIDYVMIDLFFLGGFTCLMMSSVFHCFKCHSLPVAILGNKLDYLGIVALVVTSIVSILFYGFHDYDLYFWLFSGLTMAFGLACATVSLSNKFRSREWRPYRASLFVAFGLSALLPVIAGALHYGLKETWTRIQLEWVLLGGVFYILGAVLYGIRFPERMAPGCFDIWGHSHQLFHILVVVAALCHLRALLGAYKLFHHVKVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>C5DF21</accession>
    <name>C5DF21_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Polynucleotide 5'-hydroxyl-kinase GRC3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Polynucleotide 5'-hydroxyl-kinase grc3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">CLP1</name>
        <name evidence="12" type="ordered locus">KLTH0D11594g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Required for endonucleolytic cleavage during polyadenylation-dependent pre-mRNA 3'-end formation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of a pre-mRNA cleavage factor complex. Interacts directly with PCF11.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the Clp1 family. Clp1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553214.1">
        <property type="nucleotide sequence ID" value="XM_002553168.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DF21"/>
    <dbReference type="FunCoup" id="C5DF21">
        <property type="interactions" value="858"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DF21"/>
    <dbReference type="GeneID" id="8295454"/>
    <dbReference type="KEGG" id="lth:KLTH0D11594g"/>
    <dbReference type="eggNOG" id="KOG2749">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018195_3_0_1"/>
    <dbReference type="InParanoid" id="C5DF21"/>
    <dbReference type="OMA" id="VQYVNCH"/>
    <dbReference type="OrthoDB" id="258143at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005849">
        <property type="term" value="C:mRNA cleavage factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051731">
        <property type="term" value="F:polynucleotide 5'-hydroxyl-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1030">
        <property type="entry name" value="Clp1, DNA binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.330">
        <property type="entry name" value="Pre-mRNA cleavage complex subunit Clp1, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03035">
        <property type="entry name" value="Clp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028606">
        <property type="entry name" value="Clp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045116">
        <property type="entry name" value="Clp1/Grc3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010655">
        <property type="entry name" value="Clp1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038238">
        <property type="entry name" value="Clp1_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032324">
        <property type="entry name" value="Clp1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038239">
        <property type="entry name" value="Clp1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032319">
        <property type="entry name" value="CLP1_P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12755">
        <property type="entry name" value="CLEAVAGE/POLYADENYLATION FACTOR IA SUBUNIT CLP1P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12755:SF6">
        <property type="entry name" value="POLYRIBONUCLEOTIDE 5'-HYDROXYL-KINASE CLP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06807">
        <property type="entry name" value="Clp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16573">
        <property type="entry name" value="CLP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16575">
        <property type="entry name" value="CLP1_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Clp1 N-terminal" evidence="10">
        <location>
            <begin position="28"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Clp1 P-loop" evidence="11">
        <location>
            <begin position="128"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="Clp1 C-terminal" evidence="9">
        <location>
            <begin position="336"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="131"/>
            <end position="136"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF06807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR22776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="440" mass="48476" checksum="87A6B804CAEB5E88" modified="2009-07-28" version="1">MASLPGLNDSLHPADLGVDLNEPKHVSITAGSEWRVDVPQESKLTIKVHSGVAEIFGTELASDVPYVFQGTKFAVYAVEDAEFEWTSPELTSHSISSDTNMKFIYNVHFALEKLRVSSFEGPRVLIVGESCTGKTSLAKTLCAYAIKFKAHQPMMVNLNPQECIYSPPGCLTATPISDVIDVSSSTWGQSMTSGATKLHNKQPIVKSFGLEQISENKERYVSTFQQLANAVRGRVQNDSTVRRSGLIIDTPPLGQLSDDFSELKEIIYQFKVNAVVVCAKDDTLALKLNEHLPVETLAIVRLPTSSGVVQTDDVYKRALQRNAIREYFYGDFNTVLSPYTIGVDSDMITVWQPKSVLVQSDSQDDLLPVEINASNLQHALVAISYASRRSSSEEVSASPILGFALIMDVNDTKRKLRVLLPVPGLLPDKAMILTAYRYLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>C5DF84</accession>
    <name>C5DF84_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="17">KLTH0D13046p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">KLTH0D13046g</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553277.1">
        <property type="nucleotide sequence ID" value="XM_002553231.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DF84">
        <property type="interactions" value="2139"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DF84"/>
    <dbReference type="GeneID" id="8295519"/>
    <dbReference type="KEGG" id="lth:KLTH0D13046g"/>
    <dbReference type="eggNOG" id="KOG0386">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000315_15_2_1"/>
    <dbReference type="InParanoid" id="C5DF84"/>
    <dbReference type="OMA" id="NPTRETN"/>
    <dbReference type="OrthoDB" id="5857104at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140008">
        <property type="term" value="F:histone H4 reader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17996">
        <property type="entry name" value="DEXHc_SMARCA2_SMARCA4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000008">
        <property type="entry name" value="Chromatin structure-remodeling complex subunit snf21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000843">
        <property type="entry name" value="Chromatin structure-remodeling complex subunit snf21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014978">
        <property type="entry name" value="Gln-Leu-Gln_QLQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014012">
        <property type="entry name" value="HSA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029295">
        <property type="entry name" value="SnAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10799">
        <property type="entry name" value="SNF2/RAD54 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07529">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14619">
        <property type="entry name" value="SnAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00951">
        <property type="entry name" value="QLQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01314">
        <property type="entry name" value="SnAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51204">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51666">
        <property type="entry name" value="QLQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 10" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="QLQ" evidence="16">
        <location>
            <begin position="192"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="HSA" evidence="15">
        <location>
            <begin position="513"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="703"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="14">
        <location>
            <begin position="1015"/>
            <end position="1178"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="12">
        <location>
            <begin position="1417"/>
            <end position="1487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="52"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="247"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="629"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1189"/>
            <end position="1209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1262"/>
            <end position="1390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1517"/>
            <end position="1540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="57"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="112"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="131"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="172"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="251"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="280"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="632"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1196"/>
            <end position="1207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1309"/>
            <end position="1341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="1357"/>
            <end position="1369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAR22839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1540" mass="176980" checksum="07156DE37087DD09" modified="2009-07-28" version="1">MEIPQRQLTKEEINRCYLRWQQLRNEHGENAANVPEYVYFTKVLQVAAKQQQKRQQEQQQGQQSHHQLQQRAPQQAPQQQQLPPQPQQPQQSQQTQAFQQIPQAHMQQSKVMPQMQAAHMPQAQQQWAPQSTNGHATMANSQPPQDHHIRTSMGQSPQQTQPPQPVQMPQQAHVQNMPSQTPPTGTGVPSSIFTPEQSELLKAQIASLKFLLGRQPIPPEVQEVIQRSLNNPPDFKQMLLSLSETVKKRQNPQQEQVLQNPQQEHTQQPPRRVVDQQQPLAHQSPHTSNSGVPTPLASQFPPSQPETGLASRANSGSGTANPEKGAESVKTEPIAEIGRPAQDTQPLKEVPPVPLEEFKRLNPDVEKIVNVTTPNMVVDSYSLPNFPDTAVEYKELFPSKDRPRVMLQPGVLPPGIDVHSALEIYQTLIALDIDTAVDSCLSDTLNEDADVETKKRATYDYYALQLLPLQKAMRGHVLQFDWYQKALLTNTHPNFLSKIRRINLQDALLTDELYRRYEIMQYERQRYEESTKLKSITDSSIEYYNAKLNRRTQRVKFGHRLISLHGNIEKEEQKRMERNAKQRLQALKANDEEAYIKLLDQTKDTRITHLLKQTNAFLDSLTKAVRSQQQYTKEKIDSHMQKEEESATPQSPSAGDVSDEEERQNIDYYNVAHRIKEEIKQQPSILVGGTLKEYQLKGLQWMVSLYNNHLNGILADEMGLGKTIQTISLLTYLYEAKNVRGPSLVIVPLSTLTNWDSEFDKWAPVIRKVAYKGSPNERKSKQGIIRSGQFDVVLTTFEYIIKERALLSKIKWVHMIIDEGHRMKNAQSKLSLTLNNYYHTDYRLILTGTPLQNNLPELWALLNFVLPKIFNSVKSFDEWFNTPFANTGGQDKIELSEEETLLVIRRLHKVLRPFLLRRLKKDVEKELPDKVEKVLKCKMSALQQKLYEQMLKHRRLFIGDINSNKMVGMRGFNNQIMQLKKICNHPFVFEDVEDQINPTRETNANIWRVAGKFELLERILPKFKATGHRILIFFQMTQIMDIMEDFLRLSGMKYLRLDGHTKSDDRTLLLNLFNDPNSEYFCFLLSTRAGGLGLNLQTADTVIIFDTDWNPHQDLQAQDRAHRIGQKNEVRILRLITENSVEEVILDRAHKKLDIDGKVIQAGKFDNKSTSEEQEALLRSLLEAEEEQKKKRALGMEEEEQMDDNELNETLARSEEELKIFAQIDEERSRTHLENGITTSLMESSELPNFYHQDIEAELDKKENDELLSGGRGTRERKSAIYEDDIPEEQWLKQFEISDGEDEPVSGPKKREASETRVPEKRAKLETEEPSELDVKEEANEKLPPPEAGQTSMKSTKGVKGRPRGRSRGSRINGKAKNGRNFVRDPDSVTESKEVREEVAAKAKQLHDFAVAYRDADDRRLADIFLVKPSRKLYPDYYLLIKYPVALETVLKHIETLAYGSLKDALDDFHLIFANARVYNTEESLIYHDSIELENAIIEKYKEMTGEEKVDFTEFDEEHASQPLVPIGNAESKEPSPAPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFB5</accession>
    <name>C5DFB5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0D13750p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0D13750g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553308.1">
        <property type="nucleotide sequence ID" value="XM_002553262.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFB5"/>
    <dbReference type="FunCoup" id="C5DFB5">
        <property type="interactions" value="56"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFB5"/>
    <dbReference type="GeneID" id="8295550"/>
    <dbReference type="KEGG" id="lth:KLTH0D13750g"/>
    <dbReference type="eggNOG" id="KOG1575">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_2_1_1"/>
    <dbReference type="InParanoid" id="C5DFB5"/>
    <dbReference type="OMA" id="YPVEETI"/>
    <dbReference type="OrthoDB" id="37537at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19077">
        <property type="entry name" value="AKR_AKR8A1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050791">
        <property type="entry name" value="Aldo-Keto_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43625">
        <property type="entry name" value="AFLATOXIN B1 ALDEHYDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43625:SF78">
        <property type="entry name" value="PYRIDOXAL REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="2">
        <location>
            <begin position="17"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR22870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="338" mass="37369" checksum="E8A9E7F9D785CDF6" modified="2009-07-28" version="1">MLSPKELKQAHLDAAHGYGLMGLTWAGGVAPKEQAFAALNKVVELTPQKPVIFNVGEFYGPDYSNLKLARSYFEAHPGLREQTVVTCKGCVDNKALVPHGDRAFVTQSIENSMKAFGGPFDIFQPARLDEKLFGDNVYPDETFETLVEYIKKGSLGGLSLSEVNAEQIRAIHAKYGEYICCVEVEFSLYHPEILTNGIVDACNELGIPIVCYSPLCRGLLTGAITSPEDLKAGDFKSMLKKFKGEALKHNLKLVDFLQKEIVSKRSDGLTLPSLALAWIRSFNSKYLGTKFVPIPGASSAKRVEQNFTSYDLTQEELAKIDGFLKTFKTAGDRYEFET</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFQ9</accession>
    <name>C5DFQ9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Histone-lysine N-methyltransferase SET5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">SET domain-containing protein 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0D17160g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-lysyl-[histone] + S-adenosyl-L-methionine = N(6)-methyl-L-lysyl-[histone] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10024"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:9846"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61929"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:10025"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR23014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553452.1">
        <property type="nucleotide sequence ID" value="XM_002553406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFQ9"/>
    <dbReference type="FunCoup" id="C5DFQ9">
        <property type="interactions" value="718"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFQ9"/>
    <dbReference type="GeneID" id="8295701"/>
    <dbReference type="KEGG" id="lth:KLTH0D17160g"/>
    <dbReference type="eggNOG" id="KOG2084">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031650_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFQ9"/>
    <dbReference type="OMA" id="CEPNVRY"/>
    <dbReference type="OrthoDB" id="438641at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042799">
        <property type="term" value="F:histone H4K20 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045814">
        <property type="term" value="P:negative regulation of gene expression, epigenetic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20071">
        <property type="entry name" value="SET_SMYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46402:SF2">
        <property type="entry name" value="HISTONE-LYSINE N-TRIMETHYLTRANSFERASE SMYD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46402">
        <property type="entry name" value="SET AND MYND DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0158">Chromosome</keyword>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SET" evidence="9">
        <location>
            <begin position="110"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00042380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR23014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="485" mass="55581" checksum="3B413B1C1C3B8451" modified="2009-07-28" version="1">MPLKLQHLCMNDIDETLQESQEGAFVPTDKQVCDDVVLLWRQEPATEDLGVSRLHSKIKERHEKWLLSERKLKQILIEHNLCFTDETKLFTYADQVTALECPHISDKHVEKVELKQSCKGRFSVFAKKDLRRDELVFYEHEPLTSILPLEKTSLAAVGRACSLCGQSLTQSTQFTMKNGLDCRMCSAVWCSKHCKKLDTTHSFLKHPMSRNKQVSASGWSSFEQFCKDNVMHAAYSVGTIHARALLDKNEGPRIKDQFEALRQISQVLRIRAADSTNIGGTFDAATGAVSLEDQSIWNKSFESFCKAFPGVEDEIDFNHYMILIGKFNLNQREGQIFTLYSYLNHNCEPNVRYELDGKTGLKLFARKDIKKGEELLTTYVNPLHGVTLRRRELLVNWGFLCDCERCNKELASREKLKSQHLFSSEKTDINAKARRKSSMKTSKPDLSELLKNGQEFNLEVPDEIGFGRRRKSVRFDDRVVAAVEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFU9</accession>
    <name>C5DFU9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0D18106p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0D18106g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR23054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553492.1">
        <property type="nucleotide sequence ID" value="XM_002553446.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFU9"/>
    <dbReference type="FunCoup" id="C5DFU9">
        <property type="interactions" value="97"/>
    </dbReference>
    <dbReference type="GeneID" id="8295744"/>
    <dbReference type="KEGG" id="lth:KLTH0D18106g"/>
    <dbReference type="eggNOG" id="ENOG502RCWJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097615_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFU9"/>
    <dbReference type="OMA" id="NDSRDYP"/>
    <dbReference type="OrthoDB" id="6614653at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="CDD" id="cd06121">
        <property type="entry name" value="cupin_YML079wp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009327">
        <property type="entry name" value="Cupin_DUF985"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039935">
        <property type="entry name" value="YML079W-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33387:SF3">
        <property type="entry name" value="DUF985 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33387">
        <property type="entry name" value="RMLC-LIKE JELLY ROLL FOLD PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06172">
        <property type="entry name" value="Cupin_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF985" evidence="1">
        <location>
            <begin position="36"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="221" mass="25281" checksum="7D376FC07841F5B2" modified="2009-07-28" version="1">MPNYFESSTVDFAANCEVEFPQFVKFPATQPPDPLQNFINHFKLEKHAENGYFKETDRSPFILELSNQETDETNETMNNCNVMPTATQKPVYKGNRNFSTLIYYMLTPDAPVSKLIMNSSRNIHILQRGKGQFVLIYPDGSIKSFKVGFDYSKGEVSQWVVPGGVWKASFMIPNEEFDNGFLISEVVVPGFEYSDYKHFPGFKELVEFVGQEKAELLKPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFZ0</accession>
    <name>C5DFZ0_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">RNA-binding protein VTS1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">RNA-binding protein vts1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0D01056g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="9">Monomer. Binds to RNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">P-body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the VTS1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552770.1">
        <property type="nucleotide sequence ID" value="XM_002552724.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFZ0"/>
    <dbReference type="FunCoup" id="C5DFZ0">
        <property type="interactions" value="61"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFZ0"/>
    <dbReference type="GeneID" id="8294990"/>
    <dbReference type="KEGG" id="lth:KLTH0D01056g"/>
    <dbReference type="eggNOG" id="KOG3791">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_595905_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFZ0"/>
    <dbReference type="OMA" id="QQNTVMD"/>
    <dbReference type="OrthoDB" id="2155283at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000289">
        <property type="term" value="P:nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09556">
        <property type="entry name" value="SAM_VTS1_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000033">
        <property type="entry name" value="Protein vts1, variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050897">
        <property type="entry name" value="SMAUG/VTS1_RNA-bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037635">
        <property type="entry name" value="VTS1_SAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12515:SF5">
        <property type="entry name" value="PROTEIN SMAUG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12515">
        <property type="entry name" value="STERILE ALPHA MOTIF DOMAIN CONTAINING PROTEIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SAM" evidence="13">
        <location>
            <begin position="300"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="183"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="32"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="199"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="219"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="274"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00073291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR22332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="372" mass="40502" checksum="8335492476545093" modified="2009-07-28" version="1">MFKEESMSFPPRVHSPVGMPRRAHPGAVLLSPQASAMNNSHSTGPGNSGVSQLPLSPQLSSQHLSLNDLLDQQQNMLVDSQRAAAGPAVVSPMLQPSSSMFLDTFTRPASSGAQVPAPQHNSMVNFAHDLNQLCSWMAMLSTSQQNSVMDNLLSSLNEEVLQHTKLKLDSLISSGYISPSVRSPQVIAPMPDRGPTPQPLTLDSLLNNNMDHHMSRQWSPIPQNPSNSQPIYDYINDLTHRPRSADPQLRRKMGSSGKPVQKKSPTQPKPNPHNGLSSPTGSNGSSASSSMNPKTLTDPKLLNNIPAWLKSLRLHKYSDALSGKPWAELVYLEDDALESMGVSALGARRKLLKAFAVVRDYKERGLIDANAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DG41</accession>
    <name>C5DG41_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0D02222p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0D02222g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552821.1">
        <property type="nucleotide sequence ID" value="XM_002552775.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DG41"/>
    <dbReference type="STRING" id="559295.C5DG41"/>
    <dbReference type="GeneID" id="8295041"/>
    <dbReference type="KEGG" id="lth:KLTH0D02222g"/>
    <dbReference type="eggNOG" id="ENOG502QW20">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008453_0_0_1"/>
    <dbReference type="InParanoid" id="C5DG41"/>
    <dbReference type="OMA" id="KAQPKCG"/>
    <dbReference type="OrthoDB" id="2943660at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050675">
        <property type="entry name" value="OAF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069">
        <property type="entry name" value="OLEATE-ACTIVATED TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069:SF29">
        <property type="entry name" value="OLEATE-ACTIVATED TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="8">
        <location>
            <begin position="31"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="794"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="798"/>
            <end position="812"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR22383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="847" mass="97096" checksum="CA1EFF358A75D08A" modified="2009-07-28" version="1">MDRNGLTNSTSSDSLELAVRSKKSRLRLSFVCRNCRKRKIKCDKAQPKCGRCAKLGLECNYDLSEQISLKKTPGRPVTIHEQLEELEHKFEDMRHSLNLSGMRAWNEDLDNRSKPIKINFFVGLQPNCFESIFKRDCKPFSDMGMIQKHKRLNPFLKFVTRSFKPLNYAVKKILQSVGDSLDPHSDELESVAEILFLTPQVREVLKQHSMNPEGITQETANAIRRCLLEKGSRGDEKSLFEPVDLEFSSSKISQAELIDQIVAVLPPRDQIEHLLAYFMKVVYPLVPYVNKSCFFRRVTETIKYSENGKVIGLDIGDYQDAIRKIGCMAIFLVVLRISHTALTLVKNLPYNGEHLDRFISVSQKCLAHLITLSHKTNEDILSCLILMRWSLIYSPTEGEVVAGSVTDMLLSLIVNHAIRIGLYRDRIQANGPKKNDKMERYFIHYRAKLWMGCLILLRSDLFLKGNFPVVSPEYAAMAPKEENPENYEDEAEAQIHRILHKQLDIYSKMSILDKLTLQVEESTDIEEIYSKIRRLEYDLQSYCPLSDVENLNNTTLENTIIQSMNNALYFKVNVMVKIYFLAIRASIVAILERQITEALTDTEHLSLRYREVTAECFESAVDLCEILCAYMSVADDDQNSPVMPDHRYILNQIVQIGIFRVSYYFIGVILTLLQVKNGLENFMWQNENKQSLVTEIEYKITLIESTSKAIFQSVQKLIHLGSHTLSDRYFTSFKQFLFLEYAMQVIQNEINPNTPSRLREVLGDIGLPVAMDYSPSDWEKFASYVKKAIGQAEPTPPFTSSDDIPSAPSTHAKSAPNDPALDSMLLFDDQVNIQNMLDGDYTWTDFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGB0</accession>
    <name>C5DGB0_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">UDP-N-acetylglucosamine transferase subunit ALG13</fullName>
            <ecNumber evidence="2 7">2.4.1.141</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5 7">Asparagine-linked glycosylation protein 13</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ALG13</name>
        <name evidence="9" type="ordered locus">KLTH0D03828g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 7">Involved in protein N-glycosylation. Essential for the second step of the dolichol-linked oligosaccharide pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>an N-acetyl-alpha-D-glucosaminyl-diphospho-di-trans,poly-cis-dolichol + UDP-N-acetyl-alpha-D-glucosamine = an N,N'-diacetylchitobiosyl-diphospho-di-trans,poly-cis-dolichol + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23380"/>
            <dbReference type="Rhea" id="RHEA-COMP:19507"/>
            <dbReference type="Rhea" id="RHEA-COMP:19510"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57269"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58427"/>
            <dbReference type="EC" id="2.4.1.141"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1 7">Heterodimer with ALG14 to form a functional enzyme.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the glycosyltransferase 28 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.141" evidence="2 7"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552890.1">
        <property type="nucleotide sequence ID" value="XM_002552844.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGB0"/>
    <dbReference type="FunCoup" id="C5DGB0">
        <property type="interactions" value="348"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGB0"/>
    <dbReference type="GeneID" id="8295114"/>
    <dbReference type="KEGG" id="lth:KLTH0D03828g"/>
    <dbReference type="eggNOG" id="KOG3349">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085408_2_0_1"/>
    <dbReference type="InParanoid" id="C5DGB0"/>
    <dbReference type="OMA" id="ILDAWKM"/>
    <dbReference type="OrthoDB" id="20273at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043541">
        <property type="term" value="C:UDP-N-acetylglucosamine transferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004577">
        <property type="term" value="F:N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007235">
        <property type="entry name" value="Glyco_trans_28_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052474">
        <property type="entry name" value="UDP-GlcNAc_transferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47043">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE TRANSFERASE SUBUNIT ALG13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47043:SF1">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE TRANSFERASE SUBUNIT ALG13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04101">
        <property type="entry name" value="Glyco_tran_28_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl transferase family 28 C-terminal" evidence="8">
        <location>
            <begin position="4"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00032061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR22452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="198" mass="21417" checksum="B1831D2E5C9BB28B" modified="2009-07-28" version="1">MGSTVVVTCGATVPFPELIAALLDKQVLDTLLRMHYTRLIVQYGRGYTSKFAQLLGCVRAHVEEPADNGASNLAAGVEVQVRGHYQSLEVCGFEFTGAIHELLRDNADLVISHAGTGSIVDALRLGKRLLVVANTSLMDNHQLQIARKFESRGHLQSAAAQPKALIAALRRVEAAPQVPLPNGYNSSFERLLMDVAQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGD6</accession>
    <name>C5DGD6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AP complex subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0D04444g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Adaptins are components of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the adaptor complexes large subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552916.1">
        <property type="nucleotide sequence ID" value="XM_002552870.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGD6"/>
    <dbReference type="FunCoup" id="C5DGD6">
        <property type="interactions" value="885"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGD6"/>
    <dbReference type="GeneID" id="8295142"/>
    <dbReference type="KEGG" id="lth:KLTH0D04444g"/>
    <dbReference type="eggNOG" id="KOG1061">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006320_4_0_1"/>
    <dbReference type="InParanoid" id="C5DGD6"/>
    <dbReference type="OMA" id="NPPEVQW"/>
    <dbReference type="OrthoDB" id="10254310at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030117">
        <property type="term" value="C:membrane coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026739">
        <property type="entry name" value="AP_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016342">
        <property type="entry name" value="AP_complex_bsu_1_2_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11134">
        <property type="entry name" value="ADAPTOR COMPLEX SUBUNIT BETA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002291">
        <property type="entry name" value="AP_complex_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Clathrin/coatomer adaptor adaptin-like N-terminal" evidence="8">
        <location>
            <begin position="21"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="678"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="695"/>
            <end position="705"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR002291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR22478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="705" mass="79406" checksum="16B86F15A58ACB96" modified="2009-07-28" version="1">MEKMLKKFIQNATRVAPKISKKGELFELRNGLVSQYPQTRKDAIKKTIQQMTLGKDVSSLFPDILKNIATSDVEQKKLVYLYVMNYAETHPELCILAVNTFVSDAQDTNPLIRCMAIRTMSMIRVDKILEYVEIPLRKTLQDDNPYVRKTAVICVAKLFALNKELCQELGVLEDLISALEDSNPMVVANAIAALSDIYEADESVVPLPKLIQSHVSQFLLALNECTEWARIIILGALAEYTAKDSLEAQEIIDRVTPHLQHVNAAVVLASIKVVIKNLPQVQANTESPIFSKLSSALVSLMSTPPEMQYVALRNIRIVLEKYPHILSRELRIFYVKFNDPLYVKLEKIDTLVRLVDPSNLKQCTLLLAELKEYAMEFEPEFVSRAILALSQLGVKYSEPKFITKVLEIIIELCNTRDSFQDDCLVAMCNLLRHAGPDQEGMVTQVCSLAEAWSSVENLLQTDYAKCNYIWLLGQFPQKFNNVERKLQASIDSFTQEESLTQMSLLISVVKLHSILPGSMLQNLLELATTSTDEVDVRDMAIMYWRCLSVESSHDGLINELCNSSLPEIATTLDTFSPELLETLLRELSLLSSVYFKPVSTMTKPKSSSGNHLKGKQLDELQFMAQDEILKNMNEDNLLDFDNDWPAASNSNYKSEAQGSTVDYLNDIFGLSSPAPPVENSMADLSLNKKDEKQKSSNTQDLLDLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGL8</accession>
    <name>C5DGL8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Poly(A) polymerase</fullName>
            <ecNumber evidence="13">2.7.7.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ordered locus">KLTH0D06380g</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Polymerase that creates the 3'-poly(A) tail of mRNA's.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>RNA(n) + ATP = RNA(n)-3'-adenine ribonucleotide + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11332"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17347"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="ChEBI" id="CHEBI:173115"/>
            <dbReference type="EC" id="2.7.7.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="15">Binds 2 magnesium ions. Also active with manganese.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the poly(A) polymerase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.19" evidence="13"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552998.1">
        <property type="nucleotide sequence ID" value="XM_002552952.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGL8"/>
    <dbReference type="FunCoup" id="C5DGL8">
        <property type="interactions" value="1076"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGL8"/>
    <dbReference type="GeneID" id="8295229"/>
    <dbReference type="KEGG" id="lth:KLTH0D06380g"/>
    <dbReference type="eggNOG" id="KOG2245">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011511_4_1_1"/>
    <dbReference type="InParanoid" id="C5DGL8"/>
    <dbReference type="OMA" id="PAYPAMC"/>
    <dbReference type="OrthoDB" id="412748at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990817">
        <property type="term" value="F:poly(A) RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05402">
        <property type="entry name" value="NT_PAP_TUTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.590:FF:000003">
        <property type="entry name" value="Poly(A) polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.460.10:FF:000002">
        <property type="entry name" value="Poly(A) polymerase alpha, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1410.10:FF:000001">
        <property type="entry name" value="Putative poly(A) polymerase gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.590">
        <property type="entry name" value="Poly(A) polymerase predicted RNA binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011068">
        <property type="entry name" value="NuclTrfase_I-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007012">
        <property type="entry name" value="PolA_pol_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048840">
        <property type="entry name" value="PolA_pol_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007010">
        <property type="entry name" value="PolA_pol_RNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014492">
        <property type="entry name" value="PolyA_polymerase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10682">
        <property type="entry name" value="POLY A POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10682:SF10">
        <property type="entry name" value="POLYNUCLEOTIDE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04928">
        <property type="entry name" value="PAP_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20750">
        <property type="entry name" value="PAP_NTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04926">
        <property type="entry name" value="PAP_RNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018425">
        <property type="entry name" value="PolyA_polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55003">
        <property type="entry name" value="PAP/Archaeal CCA-adding enzyme, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81631">
        <property type="entry name" value="PAP/OAS1 substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0464">Manganese</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 13" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7 13 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="222"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="Poly(A) polymerase nucleotidyltransferase" evidence="20">
        <location>
            <begin position="8"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Poly(A) polymerase central" evidence="19">
        <location>
            <begin position="206"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="Poly(A) polymerase RNA-binding" evidence="18">
        <location>
            <begin position="353"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="524"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="529"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="557"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="581"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="100"/>
            <end position="102"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="233"/>
            <end position="234"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR018425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR018425-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR018425-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF04926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF04928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF20750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="CAR22560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="594" mass="67028" checksum="65C0231EDB29EBCE" modified="2009-07-28" version="1">MNQQKTYGVTGPISTAGPTAAENQLNDALIQELKKEKSFESEEDTKKRVEVLRILQNLAQEFVYQVSKKRNMSDGMAKDAGGKIFTYGSYRLGVHGPGSDIDTLVVVPKHVTREDFFTVFDQILRTRSELEEIAPVPDAFVPIIKIKFSGISIDLICARLDIAQVPVNLTLADKNLLRNLDEKDLRALNGTRVTDEILQLVPKPTSFKIALRAIKLWAQRRAVYANIFGFPGGVAWAMLVARICQLYPNACSAVILTRFFHILTKWNWPQPVLLKPIEDGPLQVRVWNPRIYAQDRSHKMPVITPAYPSMCATHNISESTKKVILAELERGAQISSEIFSNKKTWSDLFQKHDFFYKYKFYLTVMASTSGSSEQHLKWSGLVESKLRLLVQKLETLGGINLAHPFTKPFEASYVYDNEAQCKDIIDNYGTHKAQDILAQYTEVTDDNKDTDGVKDKAQVHITTMYIGLDVALDGKKQVDIHVPCSDFFNLCRSFSEYDDTDMFSLMIKYVKLYDLPDNVYVEGEERPVKHSKRKKLGKDSKKSKRPKSNSLKAEEPTSGTTNSEKNNSQGPNKPVKHEPPMQTSPNPLAGSQVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGU4</accession>
    <name>C5DGU4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0D08360p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0D08360g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553074.1">
        <property type="nucleotide sequence ID" value="XM_002553028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGU4"/>
    <dbReference type="FunCoup" id="C5DGU4">
        <property type="interactions" value="679"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGU4"/>
    <dbReference type="GeneID" id="8295310"/>
    <dbReference type="KEGG" id="lth:KLTH0D08360g"/>
    <dbReference type="eggNOG" id="KOG2830">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041824_2_2_1"/>
    <dbReference type="InParanoid" id="C5DGU4"/>
    <dbReference type="OMA" id="EYELCEA"/>
    <dbReference type="OrthoDB" id="10261753at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051721">
        <property type="term" value="F:protein phosphatase 2A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035303">
        <property type="term" value="P:regulation of dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.540">
        <property type="entry name" value="TAP42-like family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038511">
        <property type="entry name" value="TAP42/TAP46-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007304">
        <property type="entry name" value="TAP46-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10933">
        <property type="entry name" value="IMMUNOGLOBULIN-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10933:SF9">
        <property type="entry name" value="IMMUNOGLOBULIN-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04177">
        <property type="entry name" value="TAP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="312"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="320"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="335"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="362" mass="41409" checksum="E22DEF71F7AA434E" modified="2009-07-28" version="1">MSSVKDVFQQLVSQVDSQIDSSPLRQDSKEFQERLTEIIGAFLELKSTVFSKLALFSDNETLEDLSTASMPLLAIDFYLGKLVSRKQAAQLQDTRDRNVLKLKFLRKAVQLYMQFLMSLRNYELLDKATELKLDHLEDADEPKLESLYVQPSSDKDLSGAQLKRQQKIEAYQQSKQLEAQIKALELKSSSDSNNDEDLRDLQKYQLIQTAYKALNEIEQNLYEIELLSNFITSPPPSKCIKGVTQEDSDSSRTAYTDRVESLNQPLVSKTGKVLRNFTLVNKKSQIKDKVFGYGQYAPTMSVEEFLEKELQSGRVLQGGEQDEPQPNEDDEEWQDRETYKAREWDAFKEANPKGSGNTLNRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5DH20</accession>
    <name>C5DH20_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0E00704p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0E00704g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the formin homology family. BNI1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553518.1">
        <property type="nucleotide sequence ID" value="XM_002553472.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DH20">
        <property type="interactions" value="286"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DH20"/>
    <dbReference type="GeneID" id="8291656"/>
    <dbReference type="KEGG" id="lth:KLTH0E00704g"/>
    <dbReference type="eggNOG" id="KOG1922">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002339_0_0_1"/>
    <dbReference type="InParanoid" id="C5DH20"/>
    <dbReference type="OMA" id="RKWELIC"/>
    <dbReference type="OrthoDB" id="1104827at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005522">
        <property type="term" value="F:profilin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045010">
        <property type="term" value="P:actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051016">
        <property type="term" value="P:barbed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071474">
        <property type="term" value="P:cellular hyperosmotic response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070649">
        <property type="term" value="P:formin-nucleated actin cable assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903475">
        <property type="term" value="P:mitotic actomyosin contractile ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.2220:FF:000006">
        <property type="entry name" value="Cytokinesis protein sepA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.30.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2220">
        <property type="entry name" value="Formin, FH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051661">
        <property type="entry name" value="Actin_filament_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015425">
        <property type="entry name" value="FH2_Formin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042201">
        <property type="entry name" value="FH2_Formin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014768">
        <property type="entry name" value="GBD/FH3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010473">
        <property type="entry name" value="GTPase-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47102:SF1">
        <property type="entry name" value="BNI1-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47102">
        <property type="entry name" value="PROTEIN BNI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06371">
        <property type="entry name" value="Drf_GBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02181">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01140">
        <property type="entry name" value="Drf_GBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00498">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51444">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51232">
        <property type="entry name" value="GBD_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GBD/FH3" evidence="5">
        <location>
            <begin position="116"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="FH2" evidence="6">
        <location>
            <begin position="911"/>
            <end position="1325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="576"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="649"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="749"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="877"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1324"/>
            <end position="1343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1357"/>
            <end position="1378"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="513"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="99"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="649"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="779"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="877"/>
            <end position="889"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00037935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR23081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1389" mass="156903" checksum="B4C1427B87A2FEEE" modified="2009-07-28" version="1">MDYRRGDPWIEPHGYAYRFFSLDEGIGRPGRDIQQSEISDPVSLGLRLVGSGLVKKTKKAYASPYVSKARNFSTNDIEGPSNRACSASLEKLSQSDSSDAFSWPSTSDSSSIFDPHTTPPEEVVDQHFRELLESRAFFWGSARSGLKNLSSKRKWQLVCKERLMRDEKDNTVRQQPVAVFDEAVLENLMTRLRSGENAAASLYQLEKLLRRNDFSRGFLRSDGVARLADVLASFKREDMYVCLRCFKTLANTEEGRLSVVSEDRVVSYFCTGLIIEQVHLRVHLLSAELLLLCTYIESPGGSNTVLHHLVPLFPEWISSISQLLESSSDDITNPDFIATPNFHQTQKDYCLTSLFLINSMMQIISSFREKLEVVKKLRELGIHQLFRTMHGLEYKELEEEIEKYEFLEKEVVEKTNPELPHFLNISYGNFLSTIIQEAHHNPLEHCIFQIFEDLAHLLISRTMSDSLKALTLFRTVLNYLKEYNYGEENTKVESVISVSLNQLVDNLQSDEIAERAMSELKTAQNSIDQLNSEIQILQKERSVSKGSILSELEKIKKELFAKESLIGKLEKELHLSNQQRKNDRKKLEQAAVHKVPRLPARASSLSVFEHLKTQTLKHSHQKSPRQASLSKSSRFLSLKSVIDQQLEGENSSWQTHNKLSTPLNNSVDEVDIESTSSGSPSTEDLSCHSVADQSISNGHPDGYKLRHLGFEGHKIETLNSLVKKTPNYFPETPILSQETCPVLSNYQPNGADCKPSKHIGKSSSELEADINGNQSPSKFLPPLPSSLPAPPPPPPPLPSNLESASINSIATPQAVTQPIAVNSKTPPTAPPLPENLLASKVLVLDSNEELKNLAAPPPPPPPPPLPLALSTKKVDTTSNGFVSGATQKSAPRAPPPPSNLIGSKEVHSIVEAVTENSNLKLKQIHWDKLEDVSETIWSQEGERQDAARHLESSGFLDEIAELFRVNQSLPIKAKSETSSGKDKVSILPRDLAQQFGINLHMFSNLSVDEFVNKVLHCDRDVVNNQSVLEFFAREDLSIIPRSIASKLEPYATDHQPNEAPLLDREKLERADRIFLELCYNLRSYWRPRSLCLLTLSTYEKDYFDLIYKLQRVDDAINIIKNSAKLKDTLMMIIEIGNYMNRKQAGGIRLSSLQKLTFVKSSKDKNMSLLHAVERFLRVKCRSAYGFVEDLSRVLDLGNLMVGQIEQDFHEYTQRISGVKQSLEQGRLSKPENFHPEDRLLIKVGPKIAGATRKASLLRNQFVLTMRALENLMKLYGEDPSNIDSKNEFFQHFINFVSQFKKVAKENEEKEAVERIYIQRKELLQNRSKEHSSSSQGSASEGEDNTVDILIKRLREVNNEKSTQRRSANHNSEDHLFTRTQHLLDSIQKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>C5DH52</accession>
    <name>C5DH52_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Guanine nucleotide-binding protein alpha-2 subunit</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">GP2-alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0E01408g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
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    <sequence length="450" mass="49106" checksum="4D385BF678BD7A4B" modified="2009-07-28" version="1">MGACMSKSTDGPPAKGGGTAAAKRSRSQKIAKGARAAEEKEAAAGAGSGDRSQTGHTTDSSSGAEEKAATSAKGAAPDRPRLGGAVPDNSKRQQSVVSSNSGGSTGPSMGSLSKSGGGSTDSNRALKVLLLGSGESGKSTVLQQLKILHQNGFSNEELLEFKPFIFDNIIETGKDIINARLKFHATLEPESGLSEEDLQGVLNFNTPGAQLQQFPADLQKTLSNLWSLPSTQELITGEHRSSFYLMDSARYFMDNLPRVAAENYKPTVRDVLLSRKKTSGIFDTLVEMDSNLKLHIYDVGGQRSERKKWIHCFDNVTLIIFCVSLSEFDQTLLEDKSQNRLEESLILFDSVVNSRWFARASIVLFLNKIDVFAEKIQHVPLERFFPDYTGGQDINKAAKYILWRFVQLNRAHLNIYPHVTQATDTSNIKLVFAAIKETILENSLKDSGVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
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    <name>C5DHB9_LACTC</name>
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        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
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            <dbReference type="PubMed" id="19525356"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
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        <property type="component" value="Chromosome E"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="EngB_GTPase"/>
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        <property type="entry name" value="G_ENGB_dom"/>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="161"/>
            <end position="341"/>
        </location>
    </feature>
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            <begin position="22"/>
            <end position="53"/>
        </location>
    </feature>
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        <location>
            <begin position="22"/>
            <end position="46"/>
        </location>
    </feature>
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            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022842"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023134"/>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAR23180.1"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="353" mass="39919" checksum="F5B7681604B039C3" modified="2009-07-28" version="1">MLKVHRVSQLLRGYRIKAGTRNTANTQWRNKNLENNDTDSNPNKSSLDGGEQQISRDIKDRGAFITALHEKYNSDYKRPSKSDLSSVNHFFNSARVRYEWSASTFLDIPGERLKQEAEKNVLTQASHEVEIGPTSDDTKGDKRIKKRFGKTDGIPFELLNGLPEVAFLGRCNAGKSTLLNNITTQFSHVKLNEYAKASKKAGFTKTVNCFNIGGRFRIIDTPGYGGKSTQEQGDVTMRYLRERQELKRCFLLVSAEQGFATTDSQVVDFLTKFGIPFEVIFTKMDKIKDVSQLENVIEDSGVLELPTLPQMIFLNSETNKRYPKRYGIDALRSLILESCGLEPNAKPSKTREK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHC2</accession>
    <name>C5DHC2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">D-serine dehydratase</fullName>
            <ecNumber evidence="11">4.3.1.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">D-serine deaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0E03102g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the conversion of D-serine to pyruvate and ammonia. May play a role in D-serine detoxification.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>D-serine = pyruvate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:13977"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:35247"/>
            <dbReference type="EC" id="4.3.1.18"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:13978"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DSD1 family.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.18" evidence="11"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553620.1">
        <property type="nucleotide sequence ID" value="XM_002553574.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHC2"/>
    <dbReference type="FunCoup" id="C5DHC2">
        <property type="interactions" value="49"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHC2"/>
    <dbReference type="GeneID" id="8291765"/>
    <dbReference type="KEGG" id="lth:KLTH0E03102g"/>
    <dbReference type="eggNOG" id="ENOG502QRZ0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031639_0_0_1"/>
    <dbReference type="InParanoid" id="C5DHC2"/>
    <dbReference type="OMA" id="WPRFYGW"/>
    <dbReference type="OrthoDB" id="20198at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008721">
        <property type="term" value="F:D-serine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036088">
        <property type="term" value="P:D-serine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009636">
        <property type="term" value="P:response to toxic substance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06817">
        <property type="entry name" value="PLPDE_III_DSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.10:FF:000016">
        <property type="entry name" value="D-serine dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="entry name" value="Alanine racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.37.20">
        <property type="entry name" value="D-serine dehydratase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001608">
        <property type="entry name" value="Ala_racemase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051466">
        <property type="entry name" value="D-amino_acid_metab_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026956">
        <property type="entry name" value="D-ser_dehydrat-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042208">
        <property type="entry name" value="D-ser_dehydrat-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28004:SF2">
        <property type="entry name" value="D-SERINE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28004">
        <property type="entry name" value="ZGC:162816-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01168">
        <property type="entry name" value="Ala_racemase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14031">
        <property type="entry name" value="D-ser_dehydrat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01119">
        <property type="entry name" value="D-ser_dehydrat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="PLP-binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0216">Detoxification</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="D-serine dehydratase-like" evidence="14">
        <location>
            <begin position="297"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00051198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00055764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00066349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00069616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00075219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR23183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="415" mass="46340" checksum="D676AF77EE55028F" modified="2009-07-28" version="1">MSYPYQFTSRSSKQELLSAYYGKDLSDLPTPSLVVDRSVFKKNCEKMLMNAEHLHADFRAHVKTHKTVEGTLLQLGSDERRTDKIVVSTLMEAWGLLPLIEKGLINDVLFSLPVVKSRLSELAALSERVPNLRLMLDNREQLQVLSEFSESFTFQKKWSVFIKINMGTDRAGFSDDFTLKETLCDLLKSPLKDYVSLYGFYCHAGHSYASSTEEQAKSYLLNEITSGNAACKKALEVDPSLKLQLSVGATPTAHASEMLKVSSLGKLYGRLELHAGNYPFCDLQQISTNCINSKQVSCKVLAEVVSSYPGRGLKAPGEQLVNAGVIALAREFGPLPGYGRLSCPEEYENWIVGRLSQEHGILTPLKERETAMIPLGTKVQIIPQHSCITAASYPWYFIIDNGDSVVDIWVPWRGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHC5</accession>
    <name>C5DHC5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0E03168p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0E03168g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RutC family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553623.1">
        <property type="nucleotide sequence ID" value="XM_002553577.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHC5"/>
    <dbReference type="GeneID" id="8291768"/>
    <dbReference type="KEGG" id="lth:KLTH0E03168g"/>
    <dbReference type="eggNOG" id="KOG2317">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100715_7_2_1"/>
    <dbReference type="InParanoid" id="C5DHC5"/>
    <dbReference type="OMA" id="AEGPYHQ"/>
    <dbReference type="OrthoDB" id="309640at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019239">
        <property type="term" value="F:deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00448">
        <property type="entry name" value="YjgF_YER057c_UK114_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.40">
        <property type="entry name" value="RutC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035959">
        <property type="entry name" value="RutC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006175">
        <property type="entry name" value="YjgF/YER057c/UK114"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11803">
        <property type="entry name" value="2-IMINOBUTANOATE/2-IMINOPROPANOATE DEAMINASE RIDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11803:SF58">
        <property type="entry name" value="PROTEIN HMF1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01042">
        <property type="entry name" value="Ribonuc_L-PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55298">
        <property type="entry name" value="YjgF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="148" mass="16341" checksum="0F6FCC41A8CCF4BD" modified="2009-07-28" version="1">MAQFILQVPTFIYQLHDLGTSIMVAKKITWESIEGKEHPTLSPAFVSNGHVFTSGIIGSKYGTGEVSQQIEDQVHLAIQNLENVLAVAGSSLEQVFKVTMFISHAEYSKVVNEIYGQYFPQKPARSCVVVAFMDAAIKYELEAVATLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHS4</accession>
    <name>C5DHS4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0E06732p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0E06732g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553772.1">
        <property type="nucleotide sequence ID" value="XM_002553726.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHS4"/>
    <dbReference type="FunCoup" id="C5DHS4">
        <property type="interactions" value="119"/>
    </dbReference>
    <dbReference type="GeneID" id="8291927"/>
    <dbReference type="KEGG" id="lth:KLTH0E06732g"/>
    <dbReference type="eggNOG" id="KOG0703">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031494_1_0_1"/>
    <dbReference type="InParanoid" id="C5DHS4"/>
    <dbReference type="OMA" id="RDKYEHK"/>
    <dbReference type="OrthoDB" id="10266696at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08204">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.150:FF:000027">
        <property type="entry name" value="Gts1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051718">
        <property type="entry name" value="ARF_GTPase-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45705">
        <property type="entry name" value="FI20236P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45705:SF9">
        <property type="entry name" value="PROTEIN GTS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Arf-GAP" evidence="3">
        <location>
            <begin position="7"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="79"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="134"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="143"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR23335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="351" mass="39695" checksum="93B8EB058C09A169" modified="2009-07-28" version="1">MRYKGSKAAERELRSILNSSENGNKCGECGSTYPTWCSINLGVFLCGRCASVHRKILGHRDDNVYSDVRSLTMDRWSTSDLDDISESGGNRRNKAVWNPQGVPFPFDGDEDKGAVESYIREKYVLQKFRNDRDRPVNFDSRTSRSSSRPTTSRGGSSRGSFSERPSTSSSAPVASNPPLPRRPVTNVGPRPAIFDGSDSAMSVPAAGLPAQPAVFDGFVQQYVDPATGMVYVDQRQYGMALQQQQQQEQQALQQQQQQQLLLQQQQQQLLQHQQFQAQAQKNQLLSLYQRPDLYTSAVEISPSHPQYQELMQMQQLQLQQQQQLQQQQLQMQQQQPQQTFAQGQPGFYYSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHV1</accession>
    <name>C5DHV1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 13">Tryptophan synthase</fullName>
            <ecNumber evidence="5 13">4.2.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0E07348g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-glyceraldehyde 3-phosphate + L-tryptophan + H2O</text>
            <dbReference type="Rhea" id="RHEA:10532"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.2.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the TrpB family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the TrpA family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.20" evidence="5 13"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553799.1">
        <property type="nucleotide sequence ID" value="XM_002553753.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHV1"/>
    <dbReference type="FunCoup" id="C5DHV1">
        <property type="interactions" value="519"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHV1"/>
    <dbReference type="GeneID" id="8291955"/>
    <dbReference type="KEGG" id="lth:KLTH0E07348g"/>
    <dbReference type="eggNOG" id="KOG1395">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4175">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016734_2_1_1"/>
    <dbReference type="InParanoid" id="C5DHV1"/>
    <dbReference type="OMA" id="VDTARHS"/>
    <dbReference type="OrthoDB" id="10050244at2759"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00044"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004834">
        <property type="term" value="F:tryptophan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd06446">
        <property type="entry name" value="Trp-synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04724">
        <property type="entry name" value="Tryptophan_synthase_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000151">
        <property type="entry name" value="Tryptophan synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000001">
        <property type="entry name" value="Tryptophan synthase beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000004">
        <property type="entry name" value="Tryptophan synthase beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00131">
        <property type="entry name" value="Trp_synth_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00133">
        <property type="entry name" value="Trp_synth_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006653">
        <property type="entry name" value="Trp_synth_b_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006654">
        <property type="entry name" value="Trp_synth_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023026">
        <property type="entry name" value="Trp_synth_beta/beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018204">
        <property type="entry name" value="Trp_synthase_alpha_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002028">
        <property type="entry name" value="Trp_synthase_suA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00262">
        <property type="entry name" value="trpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00263">
        <property type="entry name" value="trpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48077:SF3">
        <property type="entry name" value="TRYPTOPHAN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48077">
        <property type="entry name" value="TRYPTOPHAN SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00290">
        <property type="entry name" value="Trp_syntA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00167">
        <property type="entry name" value="TRP_SYNTHASE_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00168">
        <property type="entry name" value="TRP_SYNTHASE_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10 13" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="11 13" id="KW-0456">Lyase</keyword>
    <keyword evidence="9 13" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="14">
        <location>
            <begin position="350"/>
            <end position="675"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR23362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="709" mass="76653" checksum="FF0D5FFB7CF60A98" modified="2009-07-28" version="1">MAELLRRTFANAKSENRNALVTFMTAGYPRVEDTIPILKGFQDGGVDVIELGMPFSDPIADGPTIQVANTVALQNGVTMTQTLDMVRQARAEGVTVPIILMGYYNPILSYGEQKFIEDAAEAGASGFIIVDLPPEEAIKVRNFVDDKGLSLIPLVAPSTTDQRLELLAQIATSFIYVVSRMGTTGAQTSVADDLTELVERVRKYTKDIPLAVGFGVSTREHFTTVGSVSDGVVIGSKIIKLIEEAKEDERYITAKQYVEGILAGKKHKNLSVNEFKALQTESINAGKKNAAKAADFSEVHKFKATFGDFGGQYVPEALHACLRELEQGFESAVADPIFWKEFRDLYSYIGRPSSLHRAERLSEYCGGAQIWLKREDLNHTGSHKINNALAQVLIAKRLGKKKIIAETGAGQHGVATATACAKFGMECTVFMGAEDVRRQALNVFRMRILGAKVVAVTNGTKTLRDATSEAFRFWVSNLKTTHYVVGSAIGPHPYPILVRTFQSVIGNETKEQFAKLNDGKLPDAIVACVGGGSNSTGMFSPFEKDTSVKFLGVEAGGDGVDTKFHSATLTAGRPGVFHGVKTYVLQDVDGQVHDTHSVSAGLDYPGVGPELASWKASGRATFIAATDAQALEGFKLLSQLEGIIPALESSHAVYGAVQLAKTMDKNQNIIINVSGRGDKDVQSVAEVLPKLGPQIGWDLRFEEDPTAQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>C5DI20</accession>
    <name>C5DI20_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0E09042p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0E09042g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553868.1">
        <property type="nucleotide sequence ID" value="XM_002553822.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DI20"/>
    <dbReference type="GeneID" id="8292031"/>
    <dbReference type="KEGG" id="lth:KLTH0E09042g"/>
    <dbReference type="HOGENOM" id="CLU_1740859_0_0_1"/>
    <dbReference type="InParanoid" id="C5DI20"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="70"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="150" mass="14706" checksum="E460A4102A919EC1" modified="2009-07-28" version="1">MSAMGMEIQVDTSSSSTGRGSVSYAFMGAGGSSGTSVSVLTLDQSRMLSTTAYSSSFRFSPSGAPPTYKTPSSAAPSSGVTSSAAASLLSSSFAGFTLSTSSVPLSSASAAGQRNTTNDSSAQNGAGSCSRANWKLGAALSLLALGGVML</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIB6</accession>
    <name>C5DIB6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">cyclin-dependent kinase</fullName>
            <ecNumber evidence="2">2.7.11.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Serine/threonine-protein kinase PHO85</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0E11220g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="2"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553964.1">
        <property type="nucleotide sequence ID" value="XM_002553918.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIB6"/>
    <dbReference type="FunCoup" id="C5DIB6">
        <property type="interactions" value="577"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIB6"/>
    <dbReference type="GeneID" id="8292130"/>
    <dbReference type="KEGG" id="lth:KLTH0E11220g"/>
    <dbReference type="eggNOG" id="KOG0594">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_181_1_1"/>
    <dbReference type="InParanoid" id="C5DIB6"/>
    <dbReference type="OMA" id="NWQIFVP"/>
    <dbReference type="OrthoDB" id="1732493at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009891">
        <property type="term" value="P:positive regulation of biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000524">
        <property type="entry name" value="Cell division protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000062">
        <property type="entry name" value="PHO system negative regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF46">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="7"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR23527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="302" mass="34535" checksum="9B59FE8A0DA1D487" modified="2009-07-28" version="1">MASSSQFKQLEKLGNGTYATVYKGLNKTTGIYVALKEVKLDSEEGTPSTAVREISLMKELKHENIVRLYDVIHTENKLTLVFEFMDNDLKKYMDSRIVGNTPYGFEMSLVKYFEWQLLQGVAFCHENRILHRDLKPQNLLINNKGQLKLGDFGLARAFGIPVNTFSSEVVTLWYRAPDVLMGSRTYSTSIDIWSCGCILAEMITGRPLFPGTNDEEQLKLIFETMGTPTERTWPGVSTLPKYNPRLPQHLPKDLGALLQSQTREKMELTLIDLLYGLLQLNPDRRLSAKQALNHPWFAEYYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIG0</accession>
    <name>C5DIG0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0E12232p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0E12232g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554008.1">
        <property type="nucleotide sequence ID" value="XM_002553962.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIG0"/>
    <dbReference type="FunCoup" id="C5DIG0">
        <property type="interactions" value="45"/>
    </dbReference>
    <dbReference type="GeneID" id="8292176"/>
    <dbReference type="KEGG" id="lth:KLTH0E12232g"/>
    <dbReference type="eggNOG" id="ENOG502R67H">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017243_0_0_1"/>
    <dbReference type="InParanoid" id="C5DIG0"/>
    <dbReference type="OMA" id="VWCRQYM"/>
    <dbReference type="OrthoDB" id="5351126at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="237"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="332"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="262"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="274"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="348"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="815" mass="91696" checksum="DFCDC0065D18AE1B" modified="2009-07-28" version="1">MTGGHSHANQPINIVNSNYFRYGTSVYQKLYNGESDCSTQNSANELNETTPPAGSGTSADSDNTTSKFKRKYRSLLQNYGGNPSSKAKRFIGKLHDHGSSDSFSLFSLRSTHSNRNLPSGHSKSALPGGPQHEAISEEARPFADIKSLPVEILALVIRNFLKNACYEDARTLVRCLYVSRDFYEATKVVLYEAPGFTSTYRVAQFVTCLRVHPQNGLLVRHLDLSMLKNGVIEDQSGSFDDDEHSVGETRIPVRRRSTADSVLEEGDEEDDENERNSNRANGELERATTPVADDLPDIAWAGWRDWRYRFDPLYGNQLLSCCRTLKRVSSRSSSIHSNGTTGTGSASGYGLRRSHRSNSSVSSFTSSIMSSIYNSSSLSSLNLLSTHDPVCADGSSSWFNKLFSGKRTPKQKYQNYVKARVKKSESSEAVATPQDENAKTCVKFSVQAITKNQPYHERHPLTNKYLLKFALSKDLPLGYIFHLVRLCPNMTLLNLANLNISSDFEVQLRKDPSEIRTTSLLPNVQEIISDEDAFIGRNLEVIYLTDSSKGYSYYEGTVGNPRKRLEDLHFGSPYSMTGESWISSNYPPPIDERTKFRGQRNRHNMQNNFSLVKLEVRSLFALIHGSLPRLEKLFLDNVVWCSQMDVKGFVFSCFPQKPALHVSFIHAGMSRNMNWASSGSIHDFVAILILGEIMDRDDLFLEDLFNVRTDRYNYGGLSHDNRLVGRSNILTVTTYAGEELKCVLQIRRSSIMAVKTILDRDNNILCNVNLNSLAQVSNPSTIRIRDLTQHLTERLEDLRAADLRRHIGENQYAIH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2010-11-02" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIL2</accession>
    <name>INA17_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Inner membrane assembly complex subunit 17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">INA17</name>
        <name type="ordered locus">KLTH0E13398g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the INA complex (INAC) that promotes the biogenesis of mitochondrial F(1)F(0)-ATP synthase. INAC facilitates the assembly of the peripheral stalk and promotes the assembly of the catalytic F(1)-domain with the membrane-embedded F(0)-domain.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the inner membrane assembly (INA) complex, composed of INA17 and INA22. Interacts with a subset of F(1)F(0)-ATP synthase subunits of the F(1)-domain and the peripheral stalk.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the INA17 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554060.1">
        <property type="nucleotide sequence ID" value="XM_002554014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIL2"/>
    <dbReference type="SMR" id="C5DIL2"/>
    <dbReference type="FunCoup" id="C5DIL2">
        <property type="interactions" value="56"/>
    </dbReference>
    <dbReference type="GeneID" id="8292229"/>
    <dbReference type="KEGG" id="lth:KLTH0E13398g"/>
    <dbReference type="eggNOG" id="ENOG502S3U1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_127263_1_0_1"/>
    <dbReference type="InParanoid" id="C5DIL2"/>
    <dbReference type="OMA" id="HYVWWKL"/>
    <dbReference type="OrthoDB" id="4082954at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0175">Coiled coil</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0496">Mitochondrion</keyword>
    <keyword id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0809">Transit peptide</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="transit peptide" description="Mitochondrion" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000399880" description="Inner membrane assembly complex subunit 17" evidence="2">
        <location>
            <begin position="23"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="topological domain" description="Mitochondrial matrix" evidence="1">
        <location>
            <begin position="23"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="85"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="topological domain" description="Mitochondrial intermembrane" evidence="1">
        <location>
            <begin position="108"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="109"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q02888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="161" mass="18838" checksum="4364D814A58B4E7E" modified="2009-07-28" version="1" precursor="true">MLNPRPCVPRLLSAVARCHKPYSTSIKSLEDLAKLKSLDDVDPDLVRKLINERTNELNSQAELAMLKQMQSQENQQQQQALKKFVRPMWIFLLMSSFFYLTGHYIWWKLEYDEREIELHKQVQALRQELDSAIAAKHSGKEPALSGAGAKKPKRWYLAWLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJ36</accession>
    <name>C5DJ36_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0F13178p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0F13178g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the GDA1/CD39 NTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554762.1">
        <property type="nucleotide sequence ID" value="XM_002554716.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJ36"/>
    <dbReference type="FunCoup" id="C5DJ36">
        <property type="interactions" value="261"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJ36"/>
    <dbReference type="GeneID" id="8292986"/>
    <dbReference type="KEGG" id="lth:KLTH0F13178g"/>
    <dbReference type="eggNOG" id="KOG1386">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010246_3_3_1"/>
    <dbReference type="InParanoid" id="C5DJ36"/>
    <dbReference type="OMA" id="HESIGFM"/>
    <dbReference type="OrthoDB" id="6372431at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004382">
        <property type="term" value="F:GDP phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017111">
        <property type="term" value="F:ribonucleoside triphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045134">
        <property type="term" value="F:UDP phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046036">
        <property type="term" value="P:CTP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006256">
        <property type="term" value="P:UDP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd24039">
        <property type="entry name" value="ASKHA_NBD_YND1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.150">
        <property type="entry name" value="Exopolyphosphatase. Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000407">
        <property type="entry name" value="GDA1_CD39_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11782">
        <property type="entry name" value="ADENOSINE/GUANOSINE DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11782:SF121">
        <property type="entry name" value="NUCLEOSIDE-DIPHOSPHATASE MIG-23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01150">
        <property type="entry name" value="GDA1_CD39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01238">
        <property type="entry name" value="GDA1_CD39_NTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="514"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="188"/>
            <end position="192"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR600407-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR600407-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR24325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="658" mass="74012" checksum="AC885BF52994F07C" modified="2009-07-28" version="1">MRQSQGNALNHDQYGVVIDAGSSGSRVYIYKWQDPAYTSKVGDTENLYSVPRISLSKEWTTKTSPGLSSFKKKPNKAFKDHIKPLLQFAEDIIPKENLKSTPVFIQATAGMRLLSDKKRDLILGDLCSGIKHSTNFLLEDCRSQVQVIDGETEGIYGWIGLNYLLKNFDNYNASDELHTSFGFMDMGGGSAQVAYVPNNPREIEKHDDDISSVILRSLNGETQTWRVFVGTWLGFGANEARSRYLAQIVNSLPENTNDRNDDDFLTRELVDPCMLKGSHTKFKFKDKYFHFSGSGNFEHCSKSIFPLLRKDTPCIEEPCLFNGVHAPAIDFYNDKFIGTSEYWYTANDVFQVGGEYNFHDFSQKVKEFCESDWKEVEEHSKNGRYNNIPIKLLLDSCFKANWILSVLHEGFDMPRVGIEITDDQETKDRDTSEGAAASVAFQSTDSIEGYDLSWTLGKMVLYVSGLVEASRGDASVGISPSRNDIKIFGKDFIPGSILGQGLSATESSSLFKQFMKIIVVVVVAIIALMLWTVLFPQKASRVSRLKSFVKYLKSRLSRWWRSDLPRLSSLSSLEEGLFVRTGLESDDRDGNQVRSRSMVSLEDARKLSAEQYSMDSPVSNHSGNKFVRTNLRPAFSLADFSKFAKSAPSDRESGHVNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJ85</accession>
    <name>C5DJ85_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0F14322p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0F14322g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554811.1">
        <property type="nucleotide sequence ID" value="XM_002554765.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJ85"/>
    <dbReference type="FunCoup" id="C5DJ85">
        <property type="interactions" value="396"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJ85"/>
    <dbReference type="GeneID" id="8293037"/>
    <dbReference type="KEGG" id="lth:KLTH0F14322g"/>
    <dbReference type="eggNOG" id="KOG1274">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004219_2_0_1"/>
    <dbReference type="InParanoid" id="C5DJ85"/>
    <dbReference type="OMA" id="RYAHTNG"/>
    <dbReference type="OrthoDB" id="427368at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022100">
        <property type="entry name" value="WDHD1/CFT4_beta-prop_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048591">
        <property type="entry name" value="WDHD1/CFT4_hel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19932">
        <property type="entry name" value="WD REPEAT AND HMG-BOX DNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19932:SF10">
        <property type="entry name" value="WD REPEAT AND HMG-BOX DNA-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20946">
        <property type="entry name" value="Ctf4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12341">
        <property type="entry name" value="Mcl1_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="WDHD1/CFT4 second beta-propeller" evidence="6">
        <location>
            <begin position="460"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="domain" description="WDHD1/CFT4 helical bundle" evidence="7">
        <location>
            <begin position="792"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="376"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="391"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="417"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR24374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="900" mass="99833" checksum="9D29027E7397EBF6" modified="2009-07-28" version="1">MPSTIEKSIFSSGGKTCIGISADGTRFYAVNKNGLTKVLPLDKPEEEPDVVETCKDPSSLAVVSNSQFVVTSLKGDVYLCSVAEAQNHLLLRCALPIRDVALVHNGKTAAVGGDDLELILVGLADGSAKTRTTVTLEDQVRHLSYNQHMSILAVSQVNGSIHFYSMNSATPHHIRKLDGYIGSHFYNDNFQDKLLQSINSEEAEDIEDGAGDHEYCDQNRVCTRVAWSPQGLQFAIPCEDRSVKIFSLKDYSLSHSLSSPSIKSQFADLSFSPQTGAFIAAVDLRNRVTIWDASTGEIHFTKELPQRITNICWQLQPDNLLDLHLGTWTGDIVVIRGVAENRTAQPVPPEKAEQPQKQNGLFVSSDDEGEEAVINGSPAPLGEGLGRTIGQDDDPLGSEGLFTDEEDESSKRKTFGDEDDFIDDDDGAGYVNKRPRYTPPSIPRNAVRAIGRRPRKFRYRPFSQGATPFGSSDRRYLTMNNIGYACTVRSGDGGSSLRSTVTISFFDLGRFKEYHFEDLFNYDVCALTEEGALFAQSKLGQLHYRAHNNYQSSWTKKIPLQRSEKITAAAATPKRIIVGTSFGYIRTFNQFGIPLGVEKMAPVAAIAAHEFKVFAVHYSLHHGITYTLFEQDPKTQDRYFQRESSLPITLPSNLEAEEEFNETFSEFNPLGIKSLFFSAYGDPCIFGFDDVLLVLSKWRSAAQSRWVPLLDAQLELWKMSNGKDVNDVHVWPLGLTHNILNHILVKGYNIWPEFPMPLPSEMEVRVPLLVKDQVEVSSEGKESGEVVVPASMAAEEEFLRSKILGELLSDTLEHEGEVYGNENQILMALAGAYDKSLLRLFAAACSEQEVSKAESLALELKQDKALNAAAKVAERAELMTLVKKVNDIREARFEQQLGNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJ99</accession>
    <name>C5DJ99_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0F14718p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0F14718g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554825.1">
        <property type="nucleotide sequence ID" value="XM_002554779.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJ99"/>
    <dbReference type="FunCoup" id="C5DJ99">
        <property type="interactions" value="400"/>
    </dbReference>
    <dbReference type="GeneID" id="8293054"/>
    <dbReference type="KEGG" id="lth:KLTH0F14718g"/>
    <dbReference type="eggNOG" id="KOG2342">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047037_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJ99"/>
    <dbReference type="OMA" id="QAGGSWF"/>
    <dbReference type="OrthoDB" id="191601at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008551">
        <property type="entry name" value="TANGO2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17985">
        <property type="entry name" value="SER/THR-RICH PROTEIN T10 IN DGCR REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17985:SF8">
        <property type="entry name" value="TRANSPORT AND GOLGI ORGANIZATION PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05742">
        <property type="entry name" value="TANGO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR24388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="300" mass="33961" checksum="FE2E7BBD706EA51D" modified="2009-07-28" version="1">MCILMASVAHPKYSLIAISNRDEFFERKTSNTCLNHDDYILSPLDTALRSNNKPALGTWCGVNRKGRVAVILNLRNKEPAKRIPVKSPISRGSVPMKYLMDRGPSTDWNSFEKFVSKYPQITECEFNMFYGDCIAGEYTCIDSLGYSSEALTANKPNLVLSNDHIDSSSRWPKVQKADILLQELVQSSQHDSEEELIEKCFALASNTEPAEIDNSDKEEFTCSSIFIPPVEIPEKVDVGASLPCGRFYGTRSQIVILVKRHERTISFTERVLHDSDFEVPLHSEKHPKEKLQFNLHVDES</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJL9</accession>
    <name>C5DJL9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0F17512p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0F17512g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554945.1">
        <property type="nucleotide sequence ID" value="XM_002554899.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJL9"/>
    <dbReference type="FunCoup" id="C5DJL9">
        <property type="interactions" value="43"/>
    </dbReference>
    <dbReference type="GeneID" id="8293180"/>
    <dbReference type="KEGG" id="lth:KLTH0F17512g"/>
    <dbReference type="eggNOG" id="ENOG502RYV2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058084_1_0_1"/>
    <dbReference type="InParanoid" id="C5DJL9"/>
    <dbReference type="OMA" id="INWNKDT"/>
    <dbReference type="OrthoDB" id="5563539at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR24508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="269" mass="30752" checksum="71DFEC739B4C92BF" modified="2009-07-28" version="1">MPSYPRISLDDTVIVRRNLMLMENVTNLEKPAIDYYHFDFSSGRAMTMPQTWDLLLRLGKYQLVRLPSCSAEDDVDYQMYVKRLHHCLWRRWSMAHFGLTDCKLNPLDINWNKEQDVSVLYGPYLSLEDESRCTASTADVVHSAAPSGKPGADHADACASVDSCDDFGVAYGSSLDSRDSSIFDSLPRKSCLIRHPVRSGRHHSLHFDNQVLRRDIDKYGDLCERTVFINDAEPTLDIEFDSEYDSDYECPRAYSEECFQLDVSDSDSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJN5</accession>
    <name>C5DJN5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Thymidylate synthase</fullName>
            <ecNumber evidence="4">2.1.1.45</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0F17864g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>dUMP + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate = 7,8-dihydrofolate + dTMP</text>
            <dbReference type="Rhea" id="RHEA:12104"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57451"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <dbReference type="EC" id="2.1.1.45"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; dTTP biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the thymidylate synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.45" evidence="4"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554961.1">
        <property type="nucleotide sequence ID" value="XM_002554915.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJN5"/>
    <dbReference type="FunCoup" id="C5DJN5">
        <property type="interactions" value="633"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJN5"/>
    <dbReference type="GeneID" id="8293196"/>
    <dbReference type="KEGG" id="lth:KLTH0F17864g"/>
    <dbReference type="eggNOG" id="KOG0673">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021669_0_2_1"/>
    <dbReference type="InParanoid" id="C5DJN5"/>
    <dbReference type="OMA" id="AYGRFWR"/>
    <dbReference type="OrthoDB" id="766at2759"/>
    <dbReference type="UniPathway" id="UPA00575"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004799">
        <property type="term" value="F:thymidylate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006231">
        <property type="term" value="P:dTMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00351">
        <property type="entry name" value="TS_Pyrimidine_HMase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.572.10:FF:000005">
        <property type="entry name" value="Thymidylate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.572.10">
        <property type="entry name" value="Thymidylate synthase/dCMP hydroxymethylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00008">
        <property type="entry name" value="Thymidy_synth_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045097">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023451">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036926">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000398">
        <property type="entry name" value="Thymidylate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020940">
        <property type="entry name" value="Thymidylate_synthase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002497">
        <property type="entry name" value="PRK01827.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03284">
        <property type="entry name" value="thym_sym"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11548:SF2">
        <property type="entry name" value="THYMIDYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11548">
        <property type="entry name" value="THYMIDYLATE SYNTHASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00303">
        <property type="entry name" value="Thymidylat_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00108">
        <property type="entry name" value="THYMDSNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55831">
        <property type="entry name" value="Thymidylate synthase/dCMP hydroxymethylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00091">
        <property type="entry name" value="THYMIDYLATE_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thymidylate synthase/dCMP hydroxymethylase" evidence="11">
        <location>
            <begin position="37"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR24524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="328" mass="38319" checksum="0237A820D275868B" modified="2009-07-28" version="1">MQNQQKDTFEIQLDTFEPMTEQELKKQKLEKKNAEEQQYLDLCQRIIEEGDFRPDRTGTGTYSLFVPPQLRFDLSNDRFPLLTTKRVFTKGIILELLWFISGCTDGKKLSEQGVKIWEGNGSREYLDKLGLTDRREGDLGPVYGFQWRHFGADYKTCEDDYTNQGFDQLEDIIHRIKTNPTDRRIIMSAWNPPDFAKMALPPCHVFSQFYVTLPKKEGERPKLSCLLYQRSCDMGLGVPFNIASYALLTKMIAHVCEMEPGEFIHTMGDAHVYKDHIDALKEQIQRFPRDFPKLKIKRNVADINDFKYEDFEISEYNPHGKIQMKMSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJP5</accession>
    <name>C5DJP5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0F18084p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0F18084g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
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            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
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    </reference>
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            <end position="146"/>
        </location>
    </feature>
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        </location>
    </feature>
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        <location>
            <begin position="84"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR24534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="190" mass="20596" checksum="51671B0A8D512B98" modified="2009-07-28" version="1">MSNPPLHQSHSEPALFQTDVHHWGASALGSESTSSLLPADIDVNKLERGDLVFDRKVVTEPAENTPLLPRSVSGPPAVAPPPTAAASAPPDVARSPFPERPSRENMDPTGMSPWTNWLWSLYYGNRTMFYLVLGVLVTATTIYTAFTGEGSKYYAVILRATACYIMGTDTAQSLFGPGFCEFKAGRQTSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
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    <name>C5DK03_LACTC</name>
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        </submittedName>
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        <name evidence="2" type="ordered locus">KLTH0F00748g</name>
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        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
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            <taxon>Dikarya</taxon>
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            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
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    </organism>
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        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
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                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554241.1">
        <property type="nucleotide sequence ID" value="XM_002554195.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DK03"/>
    <dbReference type="FunCoup" id="C5DK03">
        <property type="interactions" value="83"/>
    </dbReference>
    <dbReference type="GeneID" id="8292432"/>
    <dbReference type="KEGG" id="lth:KLTH0F00748g"/>
    <dbReference type="eggNOG" id="ENOG502RXFV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026385_0_0_1"/>
    <dbReference type="InParanoid" id="C5DK03"/>
    <dbReference type="OMA" id="KERYYNY"/>
    <dbReference type="OrthoDB" id="3981301at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="185"/>
        </location>
    </feature>
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        <location>
            <begin position="208"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="329"/>
            <end position="385"/>
        </location>
    </feature>
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            <end position="66"/>
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        </location>
    </feature>
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            <end position="373"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAR23804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="404" mass="44132" checksum="EE80D86FAD2498BD" modified="2009-07-28" version="1">MMDADRLKVPSSGEPLAPGDCTPSRRGHRHKRSFAISQDLDFLRQPPGTPQEKPTHQTSPPVQHSDMSPRFFMSEESTFSHDVPNAIIDLDDALTTKPQSFTSHRRAESAPANLILPFKIEPSKPPAQPLRIEEEDSESDNEYVLHDSLMSPLRAKSQSPFLKSPSASTKSPQPARRSQYNNNALKINKQKERYLNYSKQLPAAGSQIQNQCCPQEPSSTSLSSDLTSEATTPAATVNTPNTPIFSVLKFSGRSPSPRKTFNFESQVYDLPSTDSAVSDDGKDADDGNTLTFTVTNATYEPMHRRSKSVAACEASEGLRIPKEILLGEPGGSVDLSKTVTDPKGEAAPSSKLEKPFKASVSGSYENRSVSDSAVVNDKISGSHSRRRGKLNILSSIFSRLKSSK</sequence>
</entry>
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    <name>C5DK26_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0F01254p</fullName>
        </submittedName>
    </protein>
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    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
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                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
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                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
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                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
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                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
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                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002554218.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DK26"/>
    <dbReference type="STRING" id="559295.C5DK26"/>
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    <dbReference type="KEGG" id="lth:KLTH0F01254g"/>
    <dbReference type="eggNOG" id="ENOG502RS1B">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_470955_0_0_1"/>
    <dbReference type="InParanoid" id="C5DK26"/>
    <dbReference type="OMA" id="HESVYTT"/>
    <dbReference type="OrthoDB" id="4069140at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025928">
        <property type="entry name" value="Flocculin_t3_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13928">
        <property type="entry name" value="Flocculin_t3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002947878" evidence="7">
        <location>
            <begin position="20"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
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            <begin position="136"/>
            <end position="220"/>
        </location>
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        </location>
    </feature>
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        </location>
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            <begin position="456"/>
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        </location>
    </feature>
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        <location>
            <begin position="478"/>
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        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004589"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAR23827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="580" mass="56580" checksum="36CFF62489D06984" modified="2009-07-28" version="1">MVSAKLVYGLLASSALAQAAQTYKVPIQYESELEVSYYFREDNDLVSITSDAGGLVVTVDADNACDVLQSGMVVYRVDDGSASTLLTWDRYHVFHLENSAGQELTWSSVNAQSCGAVSSSSLSSTVTLSTTYQLSSGVASSTGSGSGSITATDSGSTTASGASSGASTASGSASGSASISGSTSGSTSGSASGSASGTVSSGSTVSTSGAPSGSATKSIPGYTSSVVTVTESGVVTSYTTYCPISTVPASSTEWQSSVITATENGVVTTYTTYCPISSETATQAPEKTKVVTTTESGHESVYTTTYPASQEKTVVTTTENGVVTTYTTYCPLSSETGTAAPQETKVVTTTENGQQTIYTTTCPASQETEIVTTTKSGHESVYTTTGPAPATGATSAVTTVVQPTTIVKTITSCSGGVCSTQSATETSQVTSVVTVSKSYAAASSTETSATGVESTPATGAESTPATGAESTPATGAESTPATGAESTPATAATGTAAGSSPATGAATTGVESSASAPTGAAETGSGTAAPAATGTAASGSGSGASATPTAVSVSEYAGSAAKLGSSGVFVAVVAMMASLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DK42</accession>
    <name>C5DK42_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0F01606p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0F01606g</name>
    </gene>
    <organism evidence="10 11">
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        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
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            <taxon>Saccharomycetales</taxon>
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        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554280.1">
        <property type="nucleotide sequence ID" value="XM_002554234.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="8292471"/>
    <dbReference type="KEGG" id="lth:KLTH0F01606g"/>
    <dbReference type="eggNOG" id="KOG1286">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007946_12_0_1"/>
    <dbReference type="InParanoid" id="C5DK42"/>
    <dbReference type="OrthoDB" id="3900342at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="Amino acid permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004762">
        <property type="entry name" value="Amino_acid_permease_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00913">
        <property type="entry name" value="2A0310"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF17">
        <property type="entry name" value="GENERAL AMINO ACID PERMEASE AGP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="126"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="168"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="207"/>
            <end position="227"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="239"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
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            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
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            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="378"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="424"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="449"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="494"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="536"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="9">
        <location>
            <begin position="95"/>
            <end position="557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR23843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="604" mass="66789" checksum="12B48162D7AD0F12" modified="2009-07-28" version="1">MKGFEMKNIKSPEDGVVESFDSVEVVKETSPRSEGSRDGLFHRFVDSFKRPEEEYNGGDGIHSTHERVSGEDFERLAPDDSSKHKTLKQNIKPRHVLMISLATGIGTGMLVGNGKSLHNGGPASLLIGYAIVSTMLYCVIQSASELAIIYTSLSGGFNAYPALLVDKAFAFSVSWVYCLQWLCILPLELVTASMTIKYWNDSINPDAFVVIFYVVLIVINFIGAAGYAEAEFFFNTCKVLMLIGFFILGIIVNCGGAGNDGYLGSVYWHDPGAFRGNNGINRFKGLVAVLVNAAFAYGGAEFSVLTAAEQQNPQKSIRSASKKLVYRIIGIYLMTAALLGFLVPYNSPELLGSGSAATHASPFVIAIASHGVKVVPHIINAVILLSVLSVANSALYSSSRILLSLSEQGFAPKLFNYVDRRGRPVRCLLVSCVFGLLCFVAASPKEETVFTWLLAISGLSELFTWFSISLSHVRFRRSMIVQGRSLDELGYQSWTGVWGAYYAMIMILLILIGQFWVAISPVGSNKLDANNFFENYLAMPILIGLYLGYKLWYRDWRVIIPANEVDLVSHRKIFDAEIMQSEQLEEKEQLRHASWTKRAAEFWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DK99</accession>
    <name>C5DK99_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0F02904p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0F02904g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554337.1">
        <property type="nucleotide sequence ID" value="XM_002554291.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DK99"/>
    <dbReference type="FunCoup" id="C5DK99">
        <property type="interactions" value="307"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DK99"/>
    <dbReference type="GeneID" id="8292529"/>
    <dbReference type="KEGG" id="lth:KLTH0F02904g"/>
    <dbReference type="eggNOG" id="KOG4400">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058879_2_0_1"/>
    <dbReference type="InParanoid" id="C5DK99"/>
    <dbReference type="OMA" id="EHKQCYN"/>
    <dbReference type="OrthoDB" id="3863715at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.60.10:FF:000049">
        <property type="entry name" value="Zinc finger protein GIS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47103">
        <property type="entry name" value="DNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47103:SF8">
        <property type="entry name" value="DNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="6">
        <location>
            <begin position="8"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="6">
        <location>
            <begin position="27"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="6">
        <location>
            <begin position="51"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="6">
        <location>
            <begin position="68"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="6">
        <location>
            <begin position="104"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="6">
        <location>
            <begin position="123"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="6">
        <location>
            <begin position="146"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR23900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="162" mass="17481" checksum="529965C312FDCB01" modified="2009-07-28" version="1">MSGSQKACYVCGKIGHLAEDCESERLCYNCNQPGHVQSDCTMAKTVEFKQCYNCGETGHVKSECDVQRCYNCNQTGHISRDCADPKKPRFAGGAAPSRANKVSCYRCGGPNHMAKDCLQSDSKCYACGKVGHISKDCPAGSSAKTCYNCNEAGHISRDCPVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKQ1</accession>
    <name>C5DKQ1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ribose-phosphate diphosphokinase</fullName>
            <ecNumber evidence="3">2.7.6.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0F06556g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">5-phosphoribose 1-diphosphate synthase involved in nucleotide, histidine, and tryptophan biosynthesis. Active in heteromultimeric complexes with other 5-phosphoribose 1-diphosphate synthases (PRS2, PRS3, PRS4 and PRS5).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>D-ribose 5-phosphate + ATP = 5-phospho-alpha-D-ribose 1-diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15609"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ribose-phosphate pyrophosphokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.1" evidence="3"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554489.1">
        <property type="nucleotide sequence ID" value="XM_002554443.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKQ1"/>
    <dbReference type="FunCoup" id="C5DKQ1">
        <property type="interactions" value="241"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKQ1"/>
    <dbReference type="GeneID" id="8292690"/>
    <dbReference type="KEGG" id="lth:KLTH0F06556g"/>
    <dbReference type="eggNOG" id="KOG1448">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033546_0_1_1"/>
    <dbReference type="InParanoid" id="C5DKQ1"/>
    <dbReference type="OMA" id="MMLVGDI"/>
    <dbReference type="OrthoDB" id="413572at2759"/>
    <dbReference type="UniPathway" id="UPA00087">
        <property type="reaction ID" value="UER00172"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002189">
        <property type="term" value="C:ribose phosphate diphosphokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004749">
        <property type="term" value="F:ribose phosphate diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006015">
        <property type="term" value="P:5-phosphoribose 1-diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009156">
        <property type="term" value="P:ribonucleoside monophosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000063">
        <property type="entry name" value="Phosphoribosylpyrophosphate synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000076">
        <property type="entry name" value="Phosphoribosylpyrophosphate synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000842">
        <property type="entry name" value="PRib_PP_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029099">
        <property type="entry name" value="Pribosyltran_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005946">
        <property type="entry name" value="Rib-P_diPkinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01251">
        <property type="entry name" value="ribP_PPkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210">
        <property type="entry name" value="RIBOSE-PHOSPHATE DIPHOSPHOKINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210:SF36">
        <property type="entry name" value="RIBOSE-PHOSPHATE PYROPHOSPHOKINASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14572">
        <property type="entry name" value="Pribosyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13793">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01400">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00114">
        <property type="entry name" value="PRPP_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribose-phosphate pyrophosphokinase N-terminal" evidence="13">
        <location>
            <begin position="5"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="159"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="301"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="163"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF13793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR24052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="465" mass="50415" checksum="08A6E7A981D0D69F" modified="2009-07-28" version="1">MNNILVFGGSSHPSLVNEICANLGIKASETKLGKFSNGETSISIGESVREKDVYVIQSGCGHVNDNFMELLILLNACKTASASRVTAVMPYFCYSRQPDIPYTAKGAPLISKPKESYTFESFPGTPVPSVLTTLKPASPTGPDALNEVIEIQESTTQVLEQPTPRKLDDGDVHKSPSFSRIPMVPEGKDNSTEVGWLFNANNAGYKLWVAQSGTLIANLLTTAGADHVITMDLHDAQFQGFFDIPVDNLYCKPLAQNYIQYNIPDYKEAVIVSPDAGGAKRAASIADALELSFALIHKEKRSQLPKGPPDASLTSGGGASISNKPLISTPSSLALSSQDIKYSSSKYIQTTMLVGDVRNKTCIIVDDLVDTSYTITRAAKLLKDQGATKVYAMITHGVFSGDALNRVAQSAIDKIIVTNTVPQEKTVKYLGRDRVHVLDVSRIFGESIRRIHNGESISMLFEYGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKT0</accession>
    <name>C5DKT0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0F07238p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0F07238g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554518.1">
        <property type="nucleotide sequence ID" value="XM_002554472.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKT0"/>
    <dbReference type="GeneID" id="8292721"/>
    <dbReference type="KEGG" id="lth:KLTH0F07238g"/>
    <dbReference type="HOGENOM" id="CLU_1065850_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKT0"/>
    <dbReference type="OrthoDB" id="4033066at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="191"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="207"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="218" mass="24422" checksum="5DFB176639324E0D" modified="2009-07-28" version="1">MAFERVRKLRKRVSEISVLKRGARAAVQKKPARDGYSRQSLNYSVANLIEQAGSDYAKGETDMAIYRLTRVLSEIEKEAAESSGPSLIPLGVSEIQERPFRHLLLRNEEDFVGYSGPWYEHPCKQPCEEPVPMTDADYSQGTIASCDVGVQDDLNYTIRDADSQVFEFSDMVERSFNLLTISGGILEDEATRLDEDAPNSTSADPEAPWKEDGYHHGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5DL43</accession>
    <name>C5DL43_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0F09812p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0F09812g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the lipin family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554631.1">
        <property type="nucleotide sequence ID" value="XM_002554585.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DL43"/>
    <dbReference type="FunCoup" id="C5DL43">
        <property type="interactions" value="474"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DL43"/>
    <dbReference type="GeneID" id="8292838"/>
    <dbReference type="KEGG" id="lth:KLTH0F09812g"/>
    <dbReference type="eggNOG" id="KOG2116">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002546_3_1_1"/>
    <dbReference type="InParanoid" id="C5DL43"/>
    <dbReference type="OMA" id="QDYSMKL"/>
    <dbReference type="OrthoDB" id="4567at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008195">
        <property type="term" value="F:phosphatidate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009062">
        <property type="term" value="P:fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000063">
        <property type="entry name" value="Nuclear elongation and deformation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026058">
        <property type="entry name" value="LIPIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007651">
        <property type="entry name" value="Lipin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013209">
        <property type="entry name" value="LNS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031315">
        <property type="entry name" value="LNS2/PITP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057124">
        <property type="entry name" value="Ned1-like_M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12181">
        <property type="entry name" value="LIPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12181:SF12">
        <property type="entry name" value="PHOSPHATIDATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04571">
        <property type="entry name" value="Lipin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08235">
        <property type="entry name" value="LNS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24565">
        <property type="entry name" value="Ned1_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00775">
        <property type="entry name" value="LNS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LNS2/PITP" evidence="4">
        <location>
            <begin position="344"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="105"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="258"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="596"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="684"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="105"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="116"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="259"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="623"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="637"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="695"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="711"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="735"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="776"/>
            <end position="788"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR24194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="788" mass="86940" checksum="DE87BFFF98DA9EB6" modified="2009-07-28" version="1">MQYVGRAIGSVSKTWSSINPATLSGAIDVVVVEHPDGRLACSPFHVRFGKFQILKPSQKKVLVSVNGQPTNIPMKLGDSGEAYFVFETEGDMGTIPDELISSPVVSAASSPSASPNTNSKSQSETNLEEPDFLDIGKPAQGEVAPPSTPPPPRRPSIFEERARKLNEKLHEKQIPSKFDMNGDLLLDIEGYKPSKDRVHTTDEMLKQLLRDELGTEFDISTFVKEDHNGNMRIMNPGESNHLHSPLCSPRTLASDDFDNTVSGISESSSQTDVTTSTERDSRESLGKANSAVSEKNYIKTIRLTSEQLQCLELKYGENDLSFSVDNSRAVVTAKLFLWRWDVPLVISDIDGTITKSDALGHVLTMIGRDWTHLGVAQLFTQIKRNGYNVVYLTARSAGQADSTRSYLRSIVQDGFKLPRGPVILSPDRTMAALRREVILKKPEVFKIACLNDLRNLYVGEFDSGEKADDDRKDELPTPFMAGFGNRITDALAYRTVGIPSSRIFTINPDGEVHMELLELAGYRSSYIHINELVDHFFPPVGLNYVNCADDHRSLSPTPGSPVGTMTEERSFHRPQDVKFTDVNFWRDPIPSLADVSDLSSLESEDTRDPLDSSSRGKAPSEGWDVKSSKSDSLETDAPSTPQSARSAGHPSSASKNIGKQMYLELGSPLASPKLRYIDSKKAEDKMKSLAISSPPKASTQTSTFSVFNDGSMHDPKDTDAKFAPPGRSMNHQETSDDEFDEDEFDYEPCSSGVSDHDVNENSELDDANDNLTGNEVNDDEFDEDEFDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5DL80</accession>
    <name>C5DL80_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0F10736p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0F10736g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554668.1">
        <property type="nucleotide sequence ID" value="XM_002554622.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DL80"/>
    <dbReference type="FunCoup" id="C5DL80">
        <property type="interactions" value="268"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DL80"/>
    <dbReference type="GeneID" id="8292880"/>
    <dbReference type="KEGG" id="lth:KLTH0F10736g"/>
    <dbReference type="eggNOG" id="KOG4554">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099163_0_0_1"/>
    <dbReference type="InParanoid" id="C5DL80"/>
    <dbReference type="OMA" id="ITYSEYD"/>
    <dbReference type="OrthoDB" id="18139at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045047">
        <property type="term" value="P:protein targeting to ER"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012098">
        <property type="entry name" value="SND3_fun"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28112">
        <property type="entry name" value="SRP-INDEPENDENT TARGETING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28112:SF1">
        <property type="entry name" value="SRP-INDEPENDENT TARGETING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10032">
        <property type="entry name" value="Pho88"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF008756">
        <property type="entry name" value="P_tr_PHO88"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="170"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR24231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="190" mass="21070" checksum="B7F99702C88E37B5" modified="2009-07-28" version="1">MNPQISNLVIMLAMMQVSRRLDMEDPNIIFYVRVAYGSSVAVAWIIYQIARSRILQKNDLTTLKYVAPANAMSGETEGKLQVTTVRDYDLKELDSAVKSIYTGLAMMGFMHFYMKYTNPLLMQSISPVKTALEHNEVRIHLFGKPATGDLKRPFKAPSMFGGFGAPPAAKTDKKSIEEAERAGTGGVKAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>C5DL83</accession>
    <name>C5DL83_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0F10802p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0F10802g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554671.1">
        <property type="nucleotide sequence ID" value="XM_002554625.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DL83"/>
    <dbReference type="FunCoup" id="C5DL83">
        <property type="interactions" value="140"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DL83"/>
    <dbReference type="GeneID" id="8292883"/>
    <dbReference type="KEGG" id="lth:KLTH0F10802g"/>
    <dbReference type="eggNOG" id="ENOG502RYVU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_894829_0_0_1"/>
    <dbReference type="InParanoid" id="C5DL83"/>
    <dbReference type="OMA" id="YRPKNAN"/>
    <dbReference type="OrthoDB" id="4061106at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="CDD" id="cd23704">
        <property type="entry name" value="mS44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059185">
        <property type="entry name" value="MRP13_sacc"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR24234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="315" mass="35711" checksum="653EBF409FED1D99" modified="2009-07-28" version="1">MLRAGSRRYLSKFQSADSKLDEYYKYFATQASLRPWIYRPKNANTLLAMDLKDSESDAPLKPRAPVRPLSKQVLNTYIWSAQEASQLLSLLRKWTSLTTRKRGVWDYFTAEHIQNILFASTFKLGKLSSFLQHLYMWKPLFAEAGNDRVFDVEHFFNSLVTCQLHRNAVRELGDANVAEKKLLNAWHQVSVKENKTGLTSYLVSALAKQQGFPEVKLPGLADADVLLPHIDLGATKSGKIAAFASKHRFLYLMARTIDEYGTPSEPISSFIQNYQAAQKSLGQNDVYQEYVVIARRLNSEPHAPTVTAPHPADDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLF8</accession>
    <name>C5DLF8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0G00396p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0G00396g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555056.1">
        <property type="nucleotide sequence ID" value="XM_002555010.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLF8"/>
    <dbReference type="GeneID" id="8293314"/>
    <dbReference type="KEGG" id="lth:KLTH0G00396g"/>
    <dbReference type="HOGENOM" id="CLU_746114_0_0_1"/>
    <dbReference type="InParanoid" id="C5DLF8"/>
    <dbReference type="OrthoDB" id="10380423at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037240">
        <property type="entry name" value="ORC1-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021646">
        <property type="entry name" value="Sir1_ORC-binding"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11603">
        <property type="entry name" value="Sir1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144005">
        <property type="entry name" value="ORC1-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sir1 ORC-binding" evidence="1">
        <location>
            <begin position="4"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF11603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="371" mass="42125" checksum="1CB09B087BC3F7D5" modified="2009-07-28" version="1">MELISSRYSVVDGFLIASQQNGTKVIIDHETCCLSLNKAEKDLISAKKLRDLSRYRKSKSKLVACESQLLFEIVTAAPQVFIQNSNYTREANKGNNIKYTQSLLVIPAFFFSDVKKYFFFSQGSKGLQFQTGDTPTRPLVEEREDLFTNLELKKPKFSIHDLDVLRCYKIDQDLYAQECFSLLSFFQTKTKMSSQDVLTLETALPIFDKNLKNFDLDHCIMNKITDTVALLHFVLVNIGIFSFGHSPFLGKLSGAPIRSRIGRSYNLEVKSRYLGKLFQSDIQSYEMDGLICLLLQILAFRTDEYSDPSQILEVREAGDFCNRLAALIIWIGYAVFAESADSVISSANCKHNSSLKSLIRVYQKVKAQCDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLL8</accession>
    <name>C5DLL8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0G01716p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0G01716g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555116.1">
        <property type="nucleotide sequence ID" value="XM_002555070.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLL8"/>
    <dbReference type="FunCoup" id="C5DLL8">
        <property type="interactions" value="71"/>
    </dbReference>
    <dbReference type="GeneID" id="8293374"/>
    <dbReference type="KEGG" id="lth:KLTH0G01716g"/>
    <dbReference type="eggNOG" id="ENOG502QSM8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_301677_0_0_1"/>
    <dbReference type="InParanoid" id="C5DLL8"/>
    <dbReference type="OMA" id="QSLKYHD"/>
    <dbReference type="OrthoDB" id="3973129at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="317"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="490"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="605"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="764"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="337"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="347"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="359"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="499"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="804"/>
            <end position="834"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="845" mass="93268" checksum="63E3D3E88AFD6A41" modified="2009-07-28" version="1">MRSFIKSHKKSNSLDSSPSRSQSDKESGRSSYEYAVQPQAPLSKRPVLDKGPPQSLSFESFHKLTNKNKIFSTKIFKKPGSHTSKTGEPCTTPVSPHVPTFRNLSSPETPQSSHFSRHFQEESKEFTTIKGTRTHEWGASKDKSSSVIVLNRSSTSSDASSEMTESGFGSQVKLQRGSVSSATSSCIDENQRGSFDLQAGTTTPLVKLDDLSEVQEKHPYFSLRSHGNKAKNRQVRIHSHEDFLSLEKKSNVNLDFLPGGLGSSNRLRNLTRLNSTDDSLHCVAEKPSKAYDQAQKESTPNLNLLGLGIDAKNLVQGEESSLSSSDEREQSVITNDRSFEEGDENRASSIALSITKISVSEHPESSSERGSEPDEDYSDGSSKFSFEIGGVNGRTSSVKYYSKPNPQPHVYIDDVYEDDDFNEDMNCFDEEEDQELEMRVGVNNDNASGVRGTSKLQEKVGYGAITDAFSSDEEIEQQNSSKVKGYNDLFDISDDESESNTSFHGGQEASDGNSCFSNDEALSVKGNITTEFQSSNLKSYGDIFDLSDEELSKFPKADLSNTNEDMAAHPDVNVDFEGAEAPKQPFGHKPSTTEKIIQSCIKPVHSPKLSKPSSPRGTLRLPFPADPRSPTLDSATLFLPPPARSQVVKYHDLSSNLDYEVPGAMSNLFFIDESEEDQYNKKHQPDEHYLDEINNLPEDFAFSDDDEYYLSASRSPGSNVRNSSFKKSHSFSEKPIGAVKEKTPSNYKLELKDRVVTFFHGPFDSTLTSTSPRSPAAEEDDDTPLSIEERQIQGVTGLDPLPVTPSNSFSRPNAYLGQSNGLSPIQETNSSVDNSPRVKSRLNNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLR9</accession>
    <name>C5DLR9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein uL13m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0G02948g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the universal ribosomal protein uL13 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555167.1">
        <property type="nucleotide sequence ID" value="XM_002555121.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLR9"/>
    <dbReference type="FunCoup" id="C5DLR9">
        <property type="interactions" value="791"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLR9"/>
    <dbReference type="GeneID" id="8293430"/>
    <dbReference type="KEGG" id="lth:KLTH0G02948g"/>
    <dbReference type="eggNOG" id="KOG3203">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082184_1_1_1"/>
    <dbReference type="InParanoid" id="C5DLR9"/>
    <dbReference type="OMA" id="HKPIYTP"/>
    <dbReference type="OrthoDB" id="274622at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00392">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1180.10:FF:000007">
        <property type="entry name" value="50S ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1180.10">
        <property type="entry name" value="Ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01366">
        <property type="entry name" value="Ribosomal_uL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005822">
        <property type="entry name" value="Ribosomal_uL13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005823">
        <property type="entry name" value="Ribosomal_uL13_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023563">
        <property type="entry name" value="Ribosomal_uL13_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036899">
        <property type="entry name" value="Ribosomal_uL13_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01066">
        <property type="entry name" value="rplM_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL13M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545">
        <property type="entry name" value="RIBOSOMAL PROTEIN L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00572">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002181">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52161">
        <property type="entry name" value="Ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00783">
        <property type="entry name" value="RIBOSOMAL_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 8" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00068950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR24730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="160" mass="18175" checksum="AEA9A976C4ED882A" modified="2009-07-28" version="1">MSQKLGHTGLAFARLWHHVDIAQDKRTLGRLASSIALTLIGKHKPVYHPTQDCGDYVVVTNCQQLRVTGKKFEQKTYWSHSQKPGHLKLTTMQKMAENKGYGEILKKAVSGMLPKNKLRKPRLERLKVFDGSEHPYTSNVTAFVDQQPKIAQQLKKLEAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLT0</accession>
    <name>C5DLT0_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosphoribosylglycinamide formyltransferase</fullName>
            <ecNumber evidence="2">2.1.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">5'-phosphoribosylglycinamide transformylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">GAR transformylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0G03190g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N(1)-(5-phospho-beta-D-ribosyl)glycinamide + (6R)-10-formyltetrahydrofolate = N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide + (6S)-5,6,7,8-tetrahydrofolate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15053"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
            <dbReference type="EC" id="2.1.2.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the GART family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.2" evidence="2"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555178.1">
        <property type="nucleotide sequence ID" value="XM_002555132.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLT0"/>
    <dbReference type="FunCoup" id="C5DLT0">
        <property type="interactions" value="222"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLT0"/>
    <dbReference type="GeneID" id="8293441"/>
    <dbReference type="KEGG" id="lth:KLTH0G03190g"/>
    <dbReference type="eggNOG" id="KOG3076">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038395_0_1_1"/>
    <dbReference type="InParanoid" id="C5DLT0"/>
    <dbReference type="OMA" id="HYVDEGM"/>
    <dbReference type="OrthoDB" id="5575075at2759"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00126"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004644">
        <property type="term" value="F:phosphoribosylglycinamide formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd08645">
        <property type="entry name" value="FMT_core_GART"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.170:FF:000009">
        <property type="entry name" value="Phosphoribosylglycinamide formyltransferase (Eurofung)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.170">
        <property type="entry name" value="Formyl transferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01930">
        <property type="entry name" value="PurN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002376">
        <property type="entry name" value="Formyl_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036477">
        <property type="entry name" value="Formyl_transf_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004607">
        <property type="entry name" value="GART"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001555">
        <property type="entry name" value="GART_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00639">
        <property type="entry name" value="PurN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43369">
        <property type="entry name" value="PHOSPHORIBOSYLGLYCINAMIDE FORMYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43369:SF2">
        <property type="entry name" value="PHOSPHORIBOSYLGLYCINAMIDE FORMYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00551">
        <property type="entry name" value="Formyl_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53328">
        <property type="entry name" value="Formyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00373">
        <property type="entry name" value="GART"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Formyl transferase N-terminal" evidence="10">
        <location>
            <begin position="4"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00041324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR24741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="221" mass="23880" checksum="132DF331B972CE55" modified="2009-07-28" version="1">MGARIVVLISGSGSNLQALIDAKARGALSGDIVRVISSSKKAYGLERSSKHGIPTRVHSLYPYTKGIAKEDKSGRADARKNFEIDLASVVLEDKPDLIVCAGWLLILGSEFLKRVNGVPIINLHPALPGAFDGTTHAIEMAWKKCQDEGAPLIAGCMVHFVIEEVDRGEPVVIKELQLMPGEESLEKYEERVHAAEHVAIVEAVQKVLKANGESAGTEKQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLT7</accession>
    <name>C5DLT7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0G03366p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0G03366g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555185.1">
        <property type="nucleotide sequence ID" value="XM_002555139.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLT7"/>
    <dbReference type="FunCoup" id="C5DLT7">
        <property type="interactions" value="68"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLT7"/>
    <dbReference type="GeneID" id="8293449"/>
    <dbReference type="KEGG" id="lth:KLTH0G03366g"/>
    <dbReference type="eggNOG" id="KOG1162">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_765368_0_0_1"/>
    <dbReference type="InParanoid" id="C5DLT7"/>
    <dbReference type="OMA" id="FRRWIWI"/>
    <dbReference type="OrthoDB" id="2159384at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004342">
        <property type="entry name" value="EXS_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10783:SF46">
        <property type="entry name" value="PROTEIN ERD1 HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10783">
        <property type="entry name" value="XENOTROPIC AND POLYTROPIC RETROVIRUS RECEPTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03124">
        <property type="entry name" value="EXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51380">
        <property type="entry name" value="EXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="EXS" evidence="6">
        <location>
            <begin position="196"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR24748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="384" mass="44580" checksum="4632E38F8B8DC4E5" modified="2009-07-28" version="1">MVSKIADAPSGFLTLFPLPQRCILLIIVGMWLWLWQITIMHRFFHIDVSQLVLSHDPHEALPQQSATRQTEATRRVVSRLTKIILPWCAATSVLLSECTKIQAYSPPVWAVCLLNIQSLCQFIFIFATICSYSPMVSRCLRKIMPLGNIEAKPLRLNYILITDSLTSYSKPLIDFGFYLCHLVLDPLNEACIISRSPIGTAINLDLAIGSTPVLLRLLQCLREWRRSKYAKDARSSLFNALKYSLHIPIVLCTVYSRSYPTAKPGNHIYWLMLVNSSYSLWWDLTMDWELGIFDFSIHGMNRNEVLRRRKVFPNYMYYFAMCADFALRFVWLWELLAGRSVFEGEANIFFLQSLEILRRWIWIFIKLEAEAINSDLPEKNYNIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLU9</accession>
    <name>C5DLU9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0G03718p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0G03718g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555197.1">
        <property type="nucleotide sequence ID" value="XM_002555151.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLU9"/>
    <dbReference type="FunCoup" id="C5DLU9">
        <property type="interactions" value="386"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLU9"/>
    <dbReference type="GeneID" id="8293463"/>
    <dbReference type="KEGG" id="lth:KLTH0G03718g"/>
    <dbReference type="eggNOG" id="ENOG502QQTI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005663_0_0_1"/>
    <dbReference type="InParanoid" id="C5DLU9"/>
    <dbReference type="OMA" id="WEAGRHS"/>
    <dbReference type="OrthoDB" id="2262349at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009074">
        <property type="term" value="P:aromatic amino acid family catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052780">
        <property type="entry name" value="AAA_Catabolism_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31644">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR ARO80-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31644:SF2">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR ARO80-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="5">
        <location>
            <begin position="23"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="134"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="749"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="134"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR24760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="857" mass="97761" checksum="9C32A1D8B5E01317" modified="2009-07-28" version="1">MKRLATGNDLEVSSQKYRRGYKACLNCRSRKTRCDLGPPDSPRDPPCVRCKRERKECVFLENSKRGGRKRIEWTVDGNTDVNATARFEAEDKYIPVATLKDTTSIKEGSLTPELNSMHNALEFLAKAAGSAAKEDSRSRIPSYERQDKTIREKTYHREESPNQHASGSKRASLATPGKLIEKLSDWKPGSLYDLGDVEYIGGHKLLTEKEATRLIDLFFLNMHPFFPYIPFQLRCSQELKRYPILLCAILTISARYHNFAEHKVSEGERSSRNIEVHDRLWIYCQRLISQSVWAEASTRSVGTILAFLLFTEWNPRAIHWHWTDYANDTSLSDISRRDYHSEAKRSDNTSLTGMGAMRRSDRMAWMLTGTAVRLAQDMGFMNNSPKIFTALHITETQTAMNMNQRSILSQSLAEVSLNGHDGDSGLSSACIEEIFRNEDSKRRWKSYTQQRDSVEGKSVGFCDNEKEFLIDEYAFFHNEDSDAYRDLSNLGTHEKSKLSAADKLLFTPRQRAKVELLRIMSIGYEAIYYKDTKLTLIDHKHKLAVLGILAPMIKGWHNTYKHLLVPFAGGQCPPTRDENKNATYDTPSKIDSESLISDYFYCQLYIYSLALQIEAGTEKDKKPNINELTKSAKYVELAYNAAKEVLHSAIRVHKLRALKYMPVRWAARIIRAVAFVVRCYMTLTGSGLGSDPRATTILAVCVISAEEIIEMIQKAAITLREASPDELHLCSRFSTVLMFLCTEMKRKQHSEPQNSELRRSSSHSLHNPTDAPARMPTTPAENYAATDFAIPAFLETPHPSHLPSELEDWFCTSDDVGLDFVEPWAEMLEQQFINNDKNMTFENLYNEAMGYEHLPKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLZ6</accession>
    <name>C5DLZ6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0G04752p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0G04752g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555244.1">
        <property type="nucleotide sequence ID" value="XM_002555198.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLZ6"/>
    <dbReference type="FunCoup" id="C5DLZ6">
        <property type="interactions" value="199"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLZ6"/>
    <dbReference type="GeneID" id="8293510"/>
    <dbReference type="KEGG" id="lth:KLTH0G04752g"/>
    <dbReference type="eggNOG" id="ENOG502QUV9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_337435_0_0_1"/>
    <dbReference type="InParanoid" id="C5DLZ6"/>
    <dbReference type="OMA" id="VQINQEY"/>
    <dbReference type="OrthoDB" id="4066580at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="535"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="757"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="346"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="358"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR24807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="798" mass="90050" checksum="2F2DB5C751C8BDD9" modified="2009-07-28" version="1">MRYLAGSAINFSVWTPEPCATNIFVRAPPQATVARLLQYVHWQLSQRSESPLLPTEEYCLVLDDQPLTLSTPLQTVSAHSGGVIKLKMQRNDGILKESGTSLGYHGESDFAQTNVEFQLNVLSTDKLMSRLERGVSLNSTLAKLKKAAIGHLNDYETHNVRNLCSIKQHTVQDLVGFDILGRLHPTYLTTDDSNNDLRLYDLLGFDFSPDKFGSCCVMFRTRHDQELEDGISVRFISEASLTMDRMVVSTETTVHEMKEFICTVYGHTLRLTPADVKLVYKGQLLQEANSTGQPSKVLEYISESRGAKVHVFINQEYTEPGPGFWNELFNSSERFTFMSRSASENVSISAEEPRRTTSETNRPASMPSIQTPNAVHTSENLTQFPNSAVSVADEFTPARYDFFTESGYHIERTGERFERVLISGSEHFIPPNAFEPTVSIVKIAGIETELLPDEFSLSGGSLCLSTQAIKRIEDQLGVKLAHSVVVSLGPTPTSSSPHPSAGQAIIVPETRRMARIRQWVETIMRTVYLLLRNSVFYFVIFFQLVSFLPSFYFFLGIALIVLKAVWSTTEIWDMWRDLLWGEQADKLGEEEQVMLREILNTKPFDKQFYTRFTGNQAVTEALIDRLQNDLELREKLSGEFQLDYTESLQISVPKLFESCTATQFDQVGPESLTELFEPILNEVAGHLDNTAGLSGAGKESLKLLRAYAYQQSKLPWYKGAIRWVSHKSSSLYNGGLVEWLMPERVPRTRRGAGIRQVLHIIWDLLKLSCLFVLIVLPKFQIQAADMANRVATDEEHQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLZ9</accession>
    <name>C5DLZ9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sodium/hydrogen exchanger</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0G04818g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555247.1">
        <property type="nucleotide sequence ID" value="XM_002555201.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLZ9"/>
    <dbReference type="FunCoup" id="C5DLZ9">
        <property type="interactions" value="376"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLZ9"/>
    <dbReference type="GeneID" id="8293513"/>
    <dbReference type="KEGG" id="lth:KLTH0G04818g"/>
    <dbReference type="eggNOG" id="KOG1965">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005912_5_1_1"/>
    <dbReference type="InParanoid" id="C5DLZ9"/>
    <dbReference type="OMA" id="FTYVRRF"/>
    <dbReference type="OrthoDB" id="196264at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015386">
        <property type="term" value="F:potassium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015385">
        <property type="term" value="F:sodium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018422">
        <property type="entry name" value="Cation/H_exchanger_CPA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004709">
        <property type="entry name" value="NaH_exchanger"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00840">
        <property type="entry name" value="b_cpa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110">
        <property type="entry name" value="SODIUM/HYDROGEN EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110:SF187">
        <property type="entry name" value="SODIUM_HYDROGEN EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01084">
        <property type="entry name" value="NAHEXCHNGR"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0050">Antiport</keyword>
    <keyword evidence="6 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0915">Sodium</keyword>
    <keyword evidence="8 9" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="3 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950540" description="Sodium/hydrogen exchanger" evidence="12">
        <location>
            <begin position="22"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="62"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="93"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="122"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="151"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="187"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="257"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="314"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="345"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="376"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="457"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="13">
        <location>
            <begin position="74"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="590"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="29"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="591"/>
            <end position="612"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR24810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="612" mass="67615" checksum="01EE091C8688496B" modified="2009-07-28" version="1">MSTTFTQLVLGLAFRGALCWAKGVNTDPDDQEGVLPGDEDPPAGTLPGDEVSPVTEEIFSSWALFIMLFLLISALWSSYFLTQRRIKAVHETVLSIFYGMVVGLIIRLSPGHYIQDAVTFNSSYFFNILLPPIILNSGYELNQVNFFNNIVSILVFAIPGTFISSVVLGVILYIWTALGLESIDISFVDALSVGATLSATDPVTILSIFNAYKVDPKLYTIIFGESLLNDAISIVMFETCQKFHGKPAKFSSFFEGIGLFLMTFTVSLLIGVMIGILVALLLKHTLIRRYPRIESCLVLLIAYESYFFSNGCHMSGIVSLLFCGITLKHYAYFNMSRRTQITVKYIFQLLAQLSENFIFIYLGLSLFTEVELVYKPLLIIITGISICVARWCAVFPLSNFVNWLYRVKARRSMGIYGNDAAIPDEIPYQYQMMTFWAGLRGAVGVALAMGLQGEFKFTLLATVLVVVVLTVIIFGGTTAGMLEVLGIKTGCIDENDESDDEFDIEMPKPITLATGPIASYTDEVPSVEPISVPISTATATNPLSSDTVQSSSRVSLDKQHLRETLSTIFSADSQWFTNFDEQVLKPVFLDSDNSQSGKKSDSSTPNIMGNRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DM35</accession>
    <name>C5DM35_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0G05654p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0G05654g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555283.1">
        <property type="nucleotide sequence ID" value="XM_002555237.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DM35"/>
    <dbReference type="STRING" id="559295.C5DM35"/>
    <dbReference type="GeneID" id="8293550"/>
    <dbReference type="KEGG" id="lth:KLTH0G05654g"/>
    <dbReference type="eggNOG" id="KOG0075">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1102959_0_0_1"/>
    <dbReference type="InParanoid" id="C5DM35"/>
    <dbReference type="OMA" id="NYEYEDI"/>
    <dbReference type="OrthoDB" id="2011769at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098852">
        <property type="term" value="C:lytic vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45732">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-LIKE PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45732:SF7">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-LIKE PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 3 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="33"/>
            <end position="40"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="136"/>
            <end position="139"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR24846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="252" mass="29306" checksum="8122AC6BC3341D36" modified="2009-07-28" version="1">MLGVECLKQWATSIWQYILSIFYQKELNIVIVGLQNSGKTSLTTVLLGRPFEQDTIPTLGMRMEQFSLGLNVVRIFDLAGQHRFHHIWKRYFERADLIIYVIDLSDLTNWQECKRTLHQVIVDTNRDGIPMLILGNKIDLINIPDISKGVNDRKNQKRSAADLSAIEQWKYMAPLLRNYEFEDIPTYQLEDSNLYVLKNVETLSKELGIDLKNGILYMPESQIYLDRDLAVFTISCKDGEYVQDVLNWVNQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>C5DME3</accession>
    <name>C5DME3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0G08140p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0G08140g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555391.1">
        <property type="nucleotide sequence ID" value="XM_002555345.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DME3"/>
    <dbReference type="FunCoup" id="C5DME3">
        <property type="interactions" value="82"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DME3"/>
    <dbReference type="GeneID" id="8293662"/>
    <dbReference type="KEGG" id="lth:KLTH0G08140g"/>
    <dbReference type="eggNOG" id="ENOG502QT5E">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022324_0_0_1"/>
    <dbReference type="InParanoid" id="C5DME3"/>
    <dbReference type="OMA" id="IYIRCCK"/>
    <dbReference type="OrthoDB" id="541883at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008195">
        <property type="term" value="F:phosphatidate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017210">
        <property type="entry name" value="APP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019236">
        <property type="entry name" value="APP1_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052935">
        <property type="entry name" value="Mg2+_PAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28208">
        <property type="entry name" value="PHOSPHATIDATE PHOSPHATASE APP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28208:SF3">
        <property type="entry name" value="PHOSPHATIDATE PHOSPHATASE APP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09949">
        <property type="entry name" value="APP1_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037464">
        <property type="entry name" value="UCP037464_APP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphatidate phosphatase APP1 catalytic" evidence="2">
        <location>
            <begin position="242"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="132"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="424"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="492"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR24954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="581" mass="65299" checksum="11971D553EB22EC0" modified="2009-07-28" version="1">MNNSDEYRGTGSLNRRQRLMNIMKTTRDAYIPAFTTTLSQFRSEASSTIKGYYDSANHTGATQGVRTWPVDMKTLIYPSYTRMVDQRYFETHIRGLVFSPGTMNRKSRLILSLCRQLVRPSSPSERAALEEQLEAPLNDSQSISDSASTHSSLSLDNSDDDTLRSRISGFLHKHIMGVTLTIDISDGSSLKERTSVVTDNYGNYDVKVATQFQPQNITINIDTKDGCTVNCPINIVKDQGFALISDVDDTIKHTGVTGDKRSMFSNVFVHEFKTWAIPGMSLWYNTLKDSEGVDFFYVSNSPFQIYPVLEDYVSKHFPMGPLFLKQYSGNLVSSLMSSSAKRKLGTILGILADFPHKRFILVGDSGEGDLEAYTEAVKLFPNQILGVYIRCCKDSMSDVAAYGHKVIDELNALIEKKYYSKTNIPSTSGEVSSTRKDLTKSPSIPDLIQLDDVKTPPLKPTKKPMLTSAQRQEIESSKKAQPPLPPRKHRTFPSSIKEPSTVNRSATDDAIYYTPSSQNDYGSYPTFFDSKADSWRQRVVQTIKELKNCNAQTRLQFFTEPELCLEDSIETIRKEKARNIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMK2</accession>
    <name>C5DMK2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">histidinol-phosphate transaminase</fullName>
            <ecNumber evidence="4">2.6.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Imidazole acetol-phosphate transaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0G09614g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-histidinol phosphate + 2-oxoglutarate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:23744"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57766"/>
            <dbReference type="ChEBI" id="CHEBI:57980"/>
            <dbReference type="EC" id="2.6.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 7/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.9" evidence="4"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555450.1">
        <property type="nucleotide sequence ID" value="XM_002555404.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMK2"/>
    <dbReference type="FunCoup" id="C5DMK2">
        <property type="interactions" value="224"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMK2"/>
    <dbReference type="GeneID" id="8293728"/>
    <dbReference type="KEGG" id="lth:KLTH0G09614g"/>
    <dbReference type="eggNOG" id="KOG0633">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_3_1_1"/>
    <dbReference type="InParanoid" id="C5DMK2"/>
    <dbReference type="OMA" id="NFVQFGR"/>
    <dbReference type="OrthoDB" id="2015537at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004400">
        <property type="term" value="F:histidinol-phosphate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01023">
        <property type="entry name" value="HisC_aminotrans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001917">
        <property type="entry name" value="Aminotrans_II_pyridoxalP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005861">
        <property type="entry name" value="HisP_aminotrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01141">
        <property type="entry name" value="hisC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42885:SF2">
        <property type="entry name" value="HISTIDINOL-PHOSPHATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42885">
        <property type="entry name" value="HISTIDINOL-PHOSPHATE AMINOTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00599">
        <property type="entry name" value="AA_TRANSFER_CLASS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="9" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="12">
        <location>
            <begin position="30"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR25013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="389" mass="42769" checksum="B7B2A2C6CC6299A0" modified="2009-07-28" version="1">MTFDLSKIVRPKIFNLEPYRCARDDFTEGVLLDANENPHGPVVKDSNREFVLNRYPDPHQVAFKTKMSELRNSQSVFKDQKITPLSADNLCLGVGSDESIDALIRACCVPGKDKILIMTPTYGMYSICADINDVEIIKVPLIVEDNSFQMNTDKVIETLQGDSNIKIAFVTSPGNPTGALIDIASIEKILSQWQSGIVAIDEAYIDFCSTGGSAAPLVNKYPNLAVLQTLSKSFGLAGVRLGVTFTSKDLSRVLNAMKAPYNISSMASNLVLKALSSESVQTMKDNVQEIVEERERVLKALTSMENVQDTQVGGLDANFIMVRIVDGGKGNNELSKALYYRLATESGVVVRFRGTEYGCTGCLRITIGTHEENSKLIKEFEKKLKELTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMN9</accession>
    <name>C5DMN9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein-lysine N-methyltransferase EFM6</fullName>
            <ecNumber evidence="2">2.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Elongation factor methyltransferase 6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">EFM6</name>
        <name evidence="3" type="ordered locus">KLTH0G10428g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">S-adenosyl-L-methionine-dependent protein-lysine N-methyltransferase that methylates elongation factor 1-alpha.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class I-like SAM-binding methyltransferase superfamily. METTL21 family. EFM6 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555487.1">
        <property type="nucleotide sequence ID" value="XM_002555441.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMN9"/>
    <dbReference type="FunCoup" id="C5DMN9">
        <property type="interactions" value="429"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMN9"/>
    <dbReference type="GeneID" id="8293765"/>
    <dbReference type="KEGG" id="lth:KLTH0G10428g"/>
    <dbReference type="eggNOG" id="KOG2793">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055721_2_1_1"/>
    <dbReference type="InParanoid" id="C5DMN9"/>
    <dbReference type="OMA" id="ATHMLRD"/>
    <dbReference type="OrthoDB" id="407325at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03198">
        <property type="entry name" value="Methyltr_EFM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033684">
        <property type="entry name" value="EFM6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614:SF152">
        <property type="entry name" value="PROTEIN-LYSINE N-METHYLTRANSFERASE EFM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10294">
        <property type="entry name" value="Methyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="1 2" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="2">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="94"/>
            <end position="96"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR25050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="251" mass="27846" checksum="6E3471EA05028967" modified="2009-07-28" version="1">MAELLDFTAQFTDLVPSRPLEHFGDSDLTFGGRIFPALKIHEDGGESGCGGKLWIAGELLCEYLLENSDDNGILSKQMNLLGRDKPFVNILELGSGTGLVGLCAGSLARANGGNAKVHITDIDQLVRLMEGNVELNGLASVVSAEKLWWGEPLKYEFGPDAISNKTDLVLAADCVYLETAFPLLEKTLLDLTKGETPPVVLMSYRKRRKADRIFFKAIRKNFKVVPITSFDRCDDFLKQRTHLFQLIRVQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMU8</accession>
    <name>C5DMU8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Nuclear mRNA export factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0G11770g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus envelope</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the SAC3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555546.1">
        <property type="nucleotide sequence ID" value="XM_002555500.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMU8"/>
    <dbReference type="FunCoup" id="C5DMU8">
        <property type="interactions" value="184"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMU8"/>
    <dbReference type="GeneID" id="8293825"/>
    <dbReference type="KEGG" id="lth:KLTH0G11770g"/>
    <dbReference type="eggNOG" id="KOG1860">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006094_0_0_1"/>
    <dbReference type="InParanoid" id="C5DMU8"/>
    <dbReference type="OMA" id="FQGTCLD"/>
    <dbReference type="OrthoDB" id="264795at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070390">
        <property type="term" value="C:transcription export complex 2"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.990:FF:000008">
        <property type="entry name" value="Nuclear mRNA export protein SAC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017173">
        <property type="entry name" value="Sac3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045107">
        <property type="entry name" value="SAC3/GANP/THP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005062">
        <property type="entry name" value="SAC3/GANP/THP3_conserved"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024293">
        <property type="entry name" value="SAC3_helical"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12436">
        <property type="entry name" value="80 KDA MCM3-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12436:SF3">
        <property type="entry name" value="GERMINAL-CENTER ASSOCIATED NUCLEAR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12209">
        <property type="entry name" value="SAC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03399">
        <property type="entry name" value="SAC3_GANP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037320">
        <property type="entry name" value="mRNA_export_factor_Sac3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAC3/GANP/THP3 conserved" evidence="7">
        <location>
            <begin position="200"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="SAC3 helical" evidence="8">
        <location>
            <begin position="694"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="538"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="620"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="655"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1231"/>
            <end position="1254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="29"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="65"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="550"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="621"/>
            <end position="637"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR037320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR25109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1274" mass="144007" checksum="322C7EF04E213350" modified="2009-07-28" version="1">MSSAVRASAPSSGFSFFNNARNNGSHNGFTKKSHPQKRDKGRKQNRPGKQLSRENLSRSKPNKNASTSAGVGGSATSVKETPWTSPSKVNAAGPYSQVPPEQLGFQRIAHKPRSAPRYLIQQRPRLKPGAFTPDPWDVANQQKMLAIENEISDPTQLWETLKKMREVERGVMEDKRLVDRADSAKDLNDAIVFEGSCQDMCPVFERARRSVENNVVRYEKEDQNSKRISRFKALKVFARPAAAAAPSLPSDVRPPAVLVKTLDYIVSNIVPHLPQCEGFLWDRMRSIRQDFTYQNYSGPEAVDCNERIVRIHLLILHVMAKSDVEYSMQQELEQLHKALITLSEIYDEIRSSGGQSPNEAEFRAYSLLSRPRDPEYDKMAQSLPQDIFNDDIVQLALCFRRIISNSGYSERGHMKTENGLNLYNRFFQLIKSDRVPFLMCSFLEVYVNEIRFYALKSLSHSINRKHKPMPIEYLKEELMFNNESELFAFCEHYSIELGPDGVKVSTLSHHSHNIPETRPLKQSYLLCVEEKLQKTPTSDLINAGKPNFDTLPQAQSLNNSSKGDTALPSISSLSENNPEKQATTYTIQSPAPFSNAEMNTGFGTSLKFGALGNGSSIARNVEAKEQTENEKRDEIQRQKQLKIQQKIEEQRRLKEEREREKQYEIQQEQNRQQEEARRLARDQKELQDKASKLVASSLIQDVVGKLLQKFAKECLAQSKANQKRNELLAEELYQAFLHEKLYFISLETKADLKLQTRTLKAAWNSWGNKLAARRQKREQQKRTLDELTQVGKRLGIPEFKRPRTETPLSSFSFTLPSSSRNRLVHTPINNQESQFRTPVHKNTAIWSPIDLKTVYCDPIAERFKEKLKELKKAPEERSKISIFVFSRSWDSVSSRWLSSKFGFPVSPHQTYTSDDGRVELEVNSARPNFSTRQLENLQLLVFNSGVTEDNIFDLELKLKQDGEKLIELAHGIALNTNYRFSILIIYWESAENPLLNDEISKYLKLNKISRVFAAALDSVEVVKLTGDAPHKVLESSLDKVARHTNLELTERGAYNRSLRLKSPSASTSAYRSSRDIDEKLCSALEQERRKHEQERNRNNTYAYLQSHIAASPRTRMRKLPVLLSESHGNRFKTPLAVRPFVKRTSSYSVPSVPSHLAAKARLAREPGPAPADAALPLPADATPSYSRNQDDTTAATAAAVLPATPSLGSSARCYPGAANTSGISNVTFESPYSTPSARVLPSAGSPAAPVRDASSSVVELRDLIASVKKKLNRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMZ1</accession>
    <name>C5DMZ1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0G12826p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0G12826g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555589.1">
        <property type="nucleotide sequence ID" value="XM_002555543.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMZ1"/>
    <dbReference type="FunCoup" id="C5DMZ1">
        <property type="interactions" value="59"/>
    </dbReference>
    <dbReference type="GeneID" id="8294189"/>
    <dbReference type="KEGG" id="lth:KLTH0G12826g"/>
    <dbReference type="eggNOG" id="ENOG502QTK4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020929_1_0_1"/>
    <dbReference type="InParanoid" id="C5DMZ1"/>
    <dbReference type="OMA" id="DEHQRCN"/>
    <dbReference type="OrthoDB" id="4060589at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR25152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="524" mass="60283" checksum="D9FD97F81797AFAA" modified="2009-07-28" version="1">MLDDLPLEIIECVARYLPQGDRIRLTYVSSRLRSKVLPIIYRNIYLNETPCAPSDADPLLGNNWSCLSIPRPHFQYADSKLKALTRSLERSSLLSSYVEVVYCTWHLDLGILRGFLAVLVRKAHNLREFRNFLHLELATDLKAVGSRLRSLDLPPPGILPQKLVSKSYLSLLGDLVRHSQLDSLTSLNIYLDPGIFCSNYAPGMRKLRLRKLGLSLRGDKYNCSCFKAPRLAYSNIFDPNYLEKLTILSWHEKQDIDIYEQYHLFELLDFKNLKELTLMSFFANDSFLQECIRSFGQLRRLKLDYMFGHSLGSNIIELLAQSPSQKTLHYLDLKFWELDPYLVTIEQGRRSRFEINEICKCESCKDVFRNIIRKKLFPSQSALNIQSIEDVTKRDIITKIISVDPIVPYARFVDTRPGMLFVEDPVLGNARTINKALGVAESSESAFTRSDIVRVYHAHVHSMKRTFDYFLQKFPSLKFLSVNDVPTMVCEGPGGQHYNMPVFNSAGYLSNQIYEVVDEESLFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>C5DN32</accession>
    <name>C5DN32_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Structural maintenance of chromosomes protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0G13750g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SMC family. SMC4 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555630.1">
        <property type="nucleotide sequence ID" value="XM_002555584.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DN32">
        <property type="interactions" value="948"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DN32"/>
    <dbReference type="GeneID" id="8293913"/>
    <dbReference type="KEGG" id="lth:KLTH0G13750g"/>
    <dbReference type="eggNOG" id="KOG0996">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001042_4_1_1"/>
    <dbReference type="InParanoid" id="C5DN32"/>
    <dbReference type="OMA" id="CPALDNM"/>
    <dbReference type="OrthoDB" id="5575062at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000796">
        <property type="term" value="C:condensin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000481">
        <property type="entry name" value="Structural maintenance of chromosomes 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000585">
        <property type="entry name" value="Structural maintenance of chromosomes 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1060.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024704">
        <property type="entry name" value="SMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010935">
        <property type="entry name" value="SMC_hinge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036277">
        <property type="entry name" value="SMC_hinge_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18937:SF172">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18937">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES SMC FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06470">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005719">
        <property type="entry name" value="SMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00968">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75553">
        <property type="entry name" value="Smc hinge domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="7 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMC hinge" evidence="14">
        <location>
            <begin position="659"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1109"/>
            <end position="1167"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="313"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="380"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="538"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="836"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="1206"/>
            <end position="1243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="53"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="71"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1116"/>
            <end position="1127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="1152"/>
            <end position="1163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR005719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR25193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1399" mass="159157" checksum="F8F65B72C13DFC56" modified="2009-07-28" version="1">MESPLSKKQKTFVNRDEDFSQTEESVDVTRASRSRTPRKLVLGSPDKRFAFSQPVTSSSSNVPYLQPLKSELSSSRGRVYSQSPPRSPTRSPTRKLELIQLSPTKKTRLESQKTEQVRGLAQPIERLCINRLSLHNFKSYAGTQVVGPFHSSFSAVVGPNGSGKSNVIDSLLFVFGFRANKMRQGKLSDLIHKSEAHPNLDSCHVEVFFQYVQDELDGRTTVRQDRPGLVVTRKAFKNNTSKYYVNGKESSYTQVTELLRKEGIDLDHKRFLILQGEVESIAQMKPKAEKEGDDGLLEYLEDIIGTAKYKPLIEQTLVQIDQLNDVCQEKENRYEIVEREKSSLESGKDEALEFLEKEKKLTFLRSKLLQHKLWKNSFKSTNTQEKIKTLEEKLSAERAKYTEHKREIKQLESESKSLNVTIRGIKDSESSLTSEKRTCDRDRVSLEEKLKNISQKKVKAEKTHHATVNSINATQAKLEELFKDQVQYEKELDELNKSLLVEKTKLDEIKISLKGKTGAISVQIGEIEQELEPWNVKLQEKRSQIKLEETKISVLKESLAKIAEEISKSENDISNNRKKVAEQHQAIEGLEKEHTDIQRQIIIGQTECDNASNKMKEMKAVLTTHRQRSLDAKSSLSTFENKNKVLSALQRLQRSGRISGFHGRLGDLGTIDDKYDVAISTACPRLDDVVVETVECGQQCIEHLRKNKLGYARFILLDKLRSFNMNTIQTPNNVSRLFDLVHPIDNKFRNAFYSVLRDTLVAKDLKEANRVAYGKQRFRVVTLDGKLIDLSGTMSGGGNHRASGMMKSERLNTGASFTAEEVRQIDEELTERERNFKIATDTLHEMEDALQSLKDREPEIETDISKRRMEIDSLLSEIKLCEDRAASLKKEQKLNESSDDTLIEANSRLDSLKAECKLLEGEMKSKKNKIKELQEQIMKIGGTKLQLQGSLVDSIGQRISITLGKQKRDKTAVKKAENDLKRYEKQAGQLSVDIDQCTNELESIKELISSSDNKILELEASIDKLQKEGEEIEEKVREIERLIEEKSSEGQDFAVFEVDINNQLEKLNDLLNHIGKDGHDLLKQLESLKIRDVTATLQTLNKLLLSESRSGDELHGQSSEARSQEPSNGDLMEVDTVEGCMGNEPTASQPEDQMDVDEPEEMETSPSDLPKLTEAQLKELNVEEVELEIGQLNDFVENAYADIEVLEEYTKRLVDFQARKLELNKAVEQRDSVRQNCDNLRKKRLDEFMNGFNTISMTLKEMYQMITMGGNAELELVDSLDPFSEGVLFSVMPPKKSWRNISNLSGGEKTLSSLALVFALHKYKPTPLYVMDEIDAALDFRNVSIVANYIKERTKNAQFIVISLRNNMFELAQQLVGIYKNRNMTKSVALENKDLINRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>C5DN58</accession>
    <name>C5DN58_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="1">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0G14366g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="1"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555656.1">
        <property type="nucleotide sequence ID" value="XM_002555610.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DN58"/>
    <dbReference type="FunCoup" id="C5DN58">
        <property type="interactions" value="989"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DN58"/>
    <dbReference type="GeneID" id="8293941"/>
    <dbReference type="KEGG" id="lth:KLTH0G14366g"/>
    <dbReference type="eggNOG" id="KOG0417">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030988_13_2_1"/>
    <dbReference type="InParanoid" id="C5DN58"/>
    <dbReference type="OMA" id="AEPHEDN"/>
    <dbReference type="OrthoDB" id="7851174at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd23813">
        <property type="entry name" value="UBCc_UBE2N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000020">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="8">
        <location>
            <begin position="3"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR25219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="149" mass="16966" checksum="CBF7E41BD1A57CBD" modified="2009-07-28" version="1">MASLPKRIIKETERLVSDPVPGITAQPHEDNLRYFQVTIEGPQQSPYESGVFQLELFLPDDYPMEAPKVRFLTKIYHPNIDRLGRICLDVLKTNWSPALQIRTVLLSVQALLASPNPNDPLANDVAEDWIRNEDAAIAKAREWTQLYAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DN82</accession>
    <name>C5DN82_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Translation initiation factor eIF2B subunit beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">eIF2B GDP-GTP exchange factor subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0G14894g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="8">Component of the translation initiation factor 2B (eIF2B) complex which is a heterodecamer of two sets of five different subunits: alpha, beta, gamma, delta and epsilon. Subunits alpha, beta and delta comprise a regulatory subcomplex and subunits epsilon and gamma comprise a catalytic subcomplex. Within the complex, the hexameric regulatory complex resides at the center, with the two heterodimeric catalytic subcomplexes bound on opposite sides.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the eIF-2B alpha/beta/delta subunits family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555680.1">
        <property type="nucleotide sequence ID" value="XM_002555634.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DN82"/>
    <dbReference type="FunCoup" id="C5DN82">
        <property type="interactions" value="1144"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DN82"/>
    <dbReference type="GeneID" id="8293965"/>
    <dbReference type="KEGG" id="lth:KLTH0G14894g"/>
    <dbReference type="eggNOG" id="KOG1465">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016218_4_3_1"/>
    <dbReference type="InParanoid" id="C5DN82"/>
    <dbReference type="OMA" id="SHSCAVA"/>
    <dbReference type="OrthoDB" id="269919at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005851">
        <property type="term" value="C:eukaryotic translation initiation factor 2B complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10470:FF:000008">
        <property type="entry name" value="Translation initiation factor 2B, beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10470">
        <property type="entry name" value="Translation initiation factor eif-2b, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051855">
        <property type="entry name" value="eIF2B_beta_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000649">
        <property type="entry name" value="IF-2B-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042529">
        <property type="entry name" value="IF_2B-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45859">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45859:SF1">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01008">
        <property type="entry name" value="IF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR25243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="363" mass="40459" checksum="C8DD5FB7B9054B59" modified="2009-07-28" version="1">MSSDINVTVASFIDRLKRRQIYGSYQVSLETLQLLKRFVSAARWTNINDLISQLRALGSRLAKAQPTEFTCGNIIRRVLALIRDEVEEEIASAMVQNNAEPMISSMFSLLQKPVATPSQRSVEHPKADIRQVIIQGIKDLIDEIANIDDGIQQIAIDLIHDREILLTPTPDSKTVLKFLIKARERNNRDFTVLVSEGFPNNTHVAHQFAKKLSEHGIETTVIPDTAVFALMSRVGKVIIGAKTVFTNGGTISSTAGVSSVCECAREFKTPVFAVAGLYKLSPLYPFDIEKFVEVGGSQRVLPTMENDRVGNHNRLDTVNPIADYVPPENIDIYITNIGGFAPSFIYRVVWDNYKQQDVNLEAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNB1</accession>
    <name>C5DNB1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0G15554p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0G15554g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555709.1">
        <property type="nucleotide sequence ID" value="XM_002555663.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNB1"/>
    <dbReference type="FunCoup" id="C5DNB1">
        <property type="interactions" value="164"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNB1"/>
    <dbReference type="GeneID" id="8293995"/>
    <dbReference type="KEGG" id="lth:KLTH0G15554g"/>
    <dbReference type="eggNOG" id="ENOG502S80M">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_821512_0_0_1"/>
    <dbReference type="InParanoid" id="C5DNB1"/>
    <dbReference type="OMA" id="WFERQNE"/>
    <dbReference type="OrthoDB" id="10479290at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033194">
        <property type="entry name" value="MFAP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009730">
        <property type="entry name" value="MFAP1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15327">
        <property type="entry name" value="MICROFIBRIL-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06991">
        <property type="entry name" value="MFAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Micro-fibrillar-associated protein 1 C-terminal" evidence="2">
        <location>
            <begin position="125"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="157"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="206"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="257"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="269"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="329"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR25272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="338" mass="36694" checksum="9913A1C688F688CC" modified="2009-07-28" version="1">MAIRHFRRKPPSEEISDESSDSSSDEQSTSAQVPLDAAASRDAAQDTEPTTHLLAATPRPAASPQKPALSETAVETTIKTAGSRDALPPSVAGRSAADPSNHSASASSEESASASDSDGSEDSSSDSDSQDYALPKPVFVKNLRKRTDPATTAQNESYRREAAATRIQQEKSRLARDQQLPRASALHGTDRELTQAILELDDDDTKDPSQEHEAWQRRQEARALRTRQALEQKQKELEEREAARLASSLNDNDGFATSTVTNSTKTSTLDSRSVRSASANDASGLAHPHQRKRTSKAEMSSSKAHKKYRPQSLKGSDITLPSLDPSQQSREDNEYSVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNI1</accession>
    <name>C5DNI1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0G17226p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0G17226g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555779.1">
        <property type="nucleotide sequence ID" value="XM_002555733.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNI1"/>
    <dbReference type="STRING" id="559295.C5DNI1"/>
    <dbReference type="GeneID" id="8294071"/>
    <dbReference type="KEGG" id="lth:KLTH0G17226g"/>
    <dbReference type="eggNOG" id="ENOG502S35T">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056209_0_0_1"/>
    <dbReference type="InParanoid" id="C5DNI1"/>
    <dbReference type="OMA" id="HSNELML"/>
    <dbReference type="OrthoDB" id="4070729at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007567">
        <property type="entry name" value="Mid2_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04478">
        <property type="entry name" value="Mid2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950440" evidence="3">
        <location>
            <begin position="23"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="285"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="Mid2" evidence="4">
        <location>
            <begin position="236"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="259"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="347"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="36"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="117"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="259"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR25342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="408" mass="41569" checksum="3906C0B9775623D4" modified="2009-07-28" version="1">MVQVKFIHGVLFLAVVVKRSLQQNPDALTGANSGNSTSSVSSSSRDRSSVTSSSRTSSLSSSVSDSSLSSGTTIIVSSSLASSVSSSLASSSILSTSRTSSSSSSSRSSTSAAQTSFTSISSSSGSSPSRSSASSGSSSSSRSSRSSASSSNQSSSGSQSQSSSQSDSSSSASSTTSDSSSASTSASTSSSDSSSSSASLASSTQFSSSTPSSSSQSPSTTEAPTSSQDQQAASDSSTITRTVSGKTILSNHYTTITQSAAATAQSSSDKSGSGGSKLSKKNRNIVIGCVVGIGVPLLLAVLAFFYFYCMKTKKTNFIDSEGHVVTAYRQNKLTRWWRSAMGKEVSSEYEGDTPIGGTSDLGDEAGGHRSNGVSRNPTDGRGSHSNELMLDEEKYYDENGNELNARNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNK3</accession>
    <name>C5DNK3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">1,3-beta-glucan synthase</fullName>
            <ecNumber evidence="3">2.4.1.34</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">1,3-beta-D-glucan-UDP glucosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0G17754g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[(1-&gt;3)-beta-D-glucosyl](n) + UDP-alpha-D-glucose = [(1-&gt;3)-beta-D-glucosyl](n+1) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21476"/>
            <dbReference type="Rhea" id="RHEA-COMP:11146"/>
            <dbReference type="Rhea" id="RHEA-COMP:14303"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37671"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.4.1.34"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 48 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.34" evidence="3"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555801.1">
        <property type="nucleotide sequence ID" value="XM_002555755.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DNK3">
        <property type="interactions" value="736"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNK3"/>
    <dbReference type="GeneID" id="8294095"/>
    <dbReference type="KEGG" id="lth:KLTH0G17754g"/>
    <dbReference type="eggNOG" id="KOG0916">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000844_0_1_1"/>
    <dbReference type="InParanoid" id="C5DNK3"/>
    <dbReference type="OMA" id="AWTDFFI"/>
    <dbReference type="OrthoDB" id="1880850at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000148">
        <property type="term" value="C:1,3-beta-D-glucan synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003843">
        <property type="term" value="F:1,3-beta-D-glucan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006075">
        <property type="term" value="P:(1-&gt;3)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051278">
        <property type="term" value="P:fungal-type cell wall polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026899">
        <property type="entry name" value="FKS1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056261">
        <property type="entry name" value="FKS1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003440">
        <property type="entry name" value="Glyco_trans_48_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12741:SF48">
        <property type="entry name" value="1,3-BETA-GLUCAN SYNTHASE COMPONENT FKS1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12741">
        <property type="entry name" value="LYST-INTERACTING PROTEIN LIP5 DOPAMINE RESPONSIVE PROTEIN DRG-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14288">
        <property type="entry name" value="FKS1_dom1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23605">
        <property type="entry name" value="FKS1_dom2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02364">
        <property type="entry name" value="Glucan_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01205">
        <property type="entry name" value="FKS1_dom1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="472"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="512"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="548"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="580"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="635"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="691"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1374"/>
            <end position="1395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1461"/>
            <end position="1482"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1488"/>
            <end position="1505"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1577"/>
            <end position="1600"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1612"/>
            <end position="1635"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1655"/>
            <end position="1677"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1683"/>
            <end position="1701"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1815"/>
            <end position="1835"/>
        </location>
    </feature>
    <feature type="domain" description="1,3-beta-glucan synthase component FKS1-like" evidence="13">
        <location>
            <begin position="315"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="259"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR25364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1898" mass="217035" checksum="D89ADB3914A83779" modified="2009-07-28" version="1">MSYNDQAKYYDQQGQAEQGYDAQQGYAADPYQNYGQQAQFDEYGQPIYTEGQYDHEDAAHYDPNAADYYAQQGYEPDQADYGNGNAYYEQPRGMGQDPENFSDFSYGPPGTPGYDGYGNANAGQYTPSQMSYGEPRSSGASTPIYGGEGFDSSAIAMALPNEPYPAWTADNQSPATIEQIEDVFIDLANRFGFQRDSMRNMFDHFMTLLDSRASRMSPQQALLSLHADYIGGDTANYKKWYFAAQLDMDDEVGFRNMSLGKLSRKARKAKKKNKKAMKEADAEDTDAMLSKLEGDNSLEAADFRWKAKMNKLSPLEMVRQVALYLLIWGEANQVRFTSECLCFIYKCASDYLDSPLCQQRTEPIPEGDYLNRVITPLYLFLRSQVYEVVDGRFMKRERDHNKVIGYDDVNQLFWYPEGIAKIVFEDGSRLIDLPAEERYLRLGEVSWNDVFFKTYKEIRSWFHLVTNFNRIWVIHGCVYWMYMAYVSPTIYTKNYQQLVDNKPTPAYRWATAALGGTLACVIQIVATIAEWFFVPRNWAGAQHLSRRFMFLVLLLAINLAPVIFVFAYTGRDIYSKAANAVAGVMFFFSLGTVVFFAVMPLGGLFTSYMKKSTRKYVASQTFTASFAPLKGIDMWMSYLLWFTVFAAKYSESYFFLIKSLIDPVRILTTTTMRCTGDFWFKNKLCMHQPKIVLGLMIATDFILFFLDTFMWYVICNMIYSVGRSFYLGISILTPWRNIFTRLPKRIYSKILATTDMEIKYKPKVLISQVWNAIVISMYREHLLAIDHVQKLLYHQVPSEIEGKRTLRAPTFFVSQDDNNFETEFFPRDSEAERRISFFAQSLATPIPEPLPVDNMPTFTVLTPHYSERVLLSLREIIREDDQFSRVTLLEYLKQLHPVEWDCFVKDTKILSEETAAYEGAEEDSEKEGGLKSQIDDLPFYCIGFKSAAPEYTLRTRIWASLRSQTLYRTVSGFMNYARAIKLLYRVENPEIVQMFGGNAEGLERELEKMARRKFKFLVSMQRLAKFKAHELENAEFLLRAYPDLQIAYLDEEPPLNEGEEPRIFSALIDGHCELLPNGRRRPKFRVQLSGNPILGDGKSDNQNHAMIFYRGEYLQLIDANQDNYLEECLKIRSVLAEFEELNVEQINPYAPGLKYEEQTTNHPVAIVGAREYIFSENSGVLGDVAAGKEQTFGTLFARTLAQIGGKLHYGHPDFINATYMTTRGGVSKAQKGLHLNEDIYAGMNAVLRGGRIKHCEYYQCGKGRDLGFGTILNFTTKIGAGMGEQMLSREYYYLSTQLPLDRFLSFYYAHPGFHLNNLFIQLSVQLFMLTLMNLNALAHESIMCIYNRNKPITDVLYPLGCYNFSPVVDWVRRYTLSIFIVFFISFIPIVVQELIERGIWKATQRFFRHIISLSPMFEVFAGQIYSSSLLSDLTVGGARYISTGRGFATSRIPFSILYSRFAGSAIYMGARCMLMLLMGSVAHWQAPLLWFWASLTALMFSPFIFNPHQFSWQDFFLDYRDFIRWLSRGNNKYHRNSWIGYVRMSRSRVTGFKRKLVGDESEKGAGDAAKAHKSNMILADIIPCAIYAAGCFVAFTFINAQTGVKDADLVNSTLRIIICTLAPIAVDMGVLFFCVGMSCCSGPLFGMCCKRTGSVMAGIAHTVAVVVHVVFFIIMWLLESFNFARTLAGVATCLYCQRLIFKVMTVFMLTREFKNDHANTAFWTGKWYGGGLGYMAWTQPTREFVAKVVELSHFAGDFVLGHFILFCQLPVLCIPMIDKFHSIMLFWLKPSRQIRPPIYSLKQTRLRKRMVRKYCALYFIVLAAAVACIIGPAVASGQTKRTPFADQTEKIDPVNMKWLVNIVQPRHQDNNDTGTSLEGVSGHYYSHTPSMHSFSTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNN4</accession>
    <name>C5DNN4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0G18546p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0G18546g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555832.1">
        <property type="nucleotide sequence ID" value="XM_002555786.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNN4"/>
    <dbReference type="GeneID" id="8294127"/>
    <dbReference type="KEGG" id="lth:KLTH0G18546g"/>
    <dbReference type="eggNOG" id="KOG0719">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055868_0_2_1"/>
    <dbReference type="InParanoid" id="C5DNN4"/>
    <dbReference type="OMA" id="WLDLWSK"/>
    <dbReference type="OrthoDB" id="110024at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052594">
        <property type="entry name" value="J_domain-containing_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44144">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44144:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="3">
        <location>
            <begin position="14"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="250"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="198"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR25395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="283" mass="32988" checksum="3EC0B076D1857CC6" modified="2009-07-28" version="1">MSDSDTNIPFPDIDPYEVLGVNKSVTEKELKKCYRKLMLRCHPDKTKDWTPEAKEKFHKIQFAFEVLDKFKETYDKTGSVEACFKGSDFADWKDLFDMDVAINKDTIAADKAVYRGSTDESQDIRDSWQANAQGKVKKRYNPDEDQFTLLFQEVPHIEANESDEAYLFNLVSELLKKGEITDSDGSFERWTKNRKKYLRALQKKLAKEEKLAEEMLSQMEEKNAVSDEAELKRAIQKKNKNSFDSLISRLESQAKSKNKRRKHAEPDDEEFEKIRSKISKKRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>C5E292</accession>
    <name>C5E292_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0H03080g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556015.1">
        <property type="nucleotide sequence ID" value="XM_002555969.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E292"/>
    <dbReference type="FunCoup" id="C5E292">
        <property type="interactions" value="865"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E292"/>
    <dbReference type="GeneID" id="8294328"/>
    <dbReference type="KEGG" id="lth:KLTH0H03080g"/>
    <dbReference type="eggNOG" id="KOG2345">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_109_1_1"/>
    <dbReference type="InParanoid" id="C5E292"/>
    <dbReference type="OMA" id="AMHQYKV"/>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032889">
        <property type="term" value="P:regulation of vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006624">
        <property type="term" value="P:vacuolar protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052239">
        <property type="entry name" value="Ser/Thr-specific_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45998">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45998:SF2">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="26"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR30153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="346" mass="38122" checksum="95FFC4A034A6D391" modified="2009-07-28" version="1">MTFIGTLCGCCGSSQPSYVSVNGHRYHIKRLLGEGGFSFVYLVESRENGLMALKKIRCPFGSIGSISPAMKEVDNYQKFKSPYIAQIIDSQVVQERDGSKTVYILLPYFSKGSLQDEINRHLLDCTQVPELEVVRLALGVARGLLCIHDAAPADESDVTYSIVSAPYAEDSSLLNDLELDTFGESHQAYAHHDIKPSNIMISSEGMPVISDLGSCSRACVEINSRQALLQFQEWRSEHCTLTFMAPEILDVKLNSVITEKCDVWSFGCTIYTMCFGISPFEREEQISGASVTYSLTTGKYSIPSGTNYSSGLIDVVKKCLNVDPDGRPSVDELISLLMDLQSQLVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2B4</accession>
    <name>C5E2B4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0H03586p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0H03586g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556037.1">
        <property type="nucleotide sequence ID" value="XM_002555991.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2B4"/>
    <dbReference type="FunCoup" id="C5E2B4">
        <property type="interactions" value="19"/>
    </dbReference>
    <dbReference type="GeneID" id="8294350"/>
    <dbReference type="KEGG" id="lth:KLTH0H03586g"/>
    <dbReference type="eggNOG" id="ENOG502RY5P">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048789_0_0_1"/>
    <dbReference type="InParanoid" id="C5E2B4"/>
    <dbReference type="OrthoDB" id="4024574at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="22"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR30175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="382" mass="43512" checksum="BE2B8B9083F52266" modified="2009-07-28" version="1">MLAITNVTNDSITLPSPLRRRFSLFVCIQLFILLLSVVKPVGGLVVSSRLKKLELALATQDRICSMDHVETKTSYRELIGTLTDISFGATGHSSKIYTGFKVHRIYHFGKMLSKAKPSLQLSFCEHGKVKWQTELEYRSDIDWDLPFCVYSQDIEGVGDQGHSRIWGALKKGNRNQDTVKGPSLKELARQDTANFHCFKMARREKYTKRSTSIYLPNEWVGGLFYCDLTPDGKSRLLDQMGDDLHIDATKSHGVCNRANAIPLQPLISDSYRRHWTSLKPGTRIPFLRRITRTNMDKVIPFLHTPNSDSDFGNFTTKNDITVSKSSTKLRSNWLSAVNWLFASKVEEHLWFNEVEVEESYGVMSAQNNLTNASYIDILKIKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2C6</accession>
    <name>C5E2C6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 16">mRNA-capping enzyme subunit alpha</fullName>
            <ecNumber evidence="3 16">2.7.7.50</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12 16">GTP--RNA guanylyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13 16">mRNA guanylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ordered locus">KLTH0H03850g</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Second step of mRNA capping. Transfer of the GMP moiety of GTP to the 5'-end of RNA via an enzyme-GMP covalent reaction intermediate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 5'-end diphospho-ribonucleoside in mRNA + GTP + H(+) = a 5'-end (5'-triphosphoguanosine)-ribonucleoside in mRNA + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:67012"/>
            <dbReference type="Rhea" id="RHEA-COMP:17165"/>
            <dbReference type="Rhea" id="RHEA-COMP:17166"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:167616"/>
            <dbReference type="ChEBI" id="CHEBI:167617"/>
            <dbReference type="EC" id="2.7.7.50"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:67013"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Heterodimer. The mRNA-capping enzyme is composed of two separate chains alpha and beta, respectively a mRNA guanylyltransferase and an mRNA 5'-triphosphate monophosphatase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the eukaryotic GTase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.50" evidence="3 16"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556049.1">
        <property type="nucleotide sequence ID" value="XM_002556003.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2C6"/>
    <dbReference type="FunCoup" id="C5E2C6">
        <property type="interactions" value="470"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2C6"/>
    <dbReference type="GeneID" id="8294362"/>
    <dbReference type="KEGG" id="lth:KLTH0H03850g"/>
    <dbReference type="eggNOG" id="KOG2386">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021710_0_2_1"/>
    <dbReference type="InParanoid" id="C5E2C6"/>
    <dbReference type="OMA" id="KDYYVCE"/>
    <dbReference type="OrthoDB" id="200924at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031533">
        <property type="term" value="C:mRNA capping enzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004484">
        <property type="term" value="F:mRNA guanylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006370">
        <property type="term" value="P:7-methylguanosine mRNA capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07895">
        <property type="entry name" value="Adenylation_mRNA_capping"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000253">
        <property type="entry name" value="mRNA-capping enzyme subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.30:FF:000011">
        <property type="entry name" value="mRNA-capping enzyme subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.30">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001339">
        <property type="entry name" value="mRNA_cap_enzyme_adenylation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017075">
        <property type="entry name" value="mRNA_cap_enzyme_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013846">
        <property type="entry name" value="mRNA_cap_enzyme_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051029">
        <property type="entry name" value="mRNA_Capping_Enz/RNA_Phosphat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10367">
        <property type="entry name" value="MRNA-CAPPING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10367:SF17">
        <property type="entry name" value="MRNA-CAPPING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03919">
        <property type="entry name" value="mRNA_cap_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01331">
        <property type="entry name" value="mRNA_cap_enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036959">
        <property type="entry name" value="mRNA_cap_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56091">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 16" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9 16" id="KW-0506">mRNA capping</keyword>
    <keyword evidence="5 16" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 16" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11 16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="mRNA capping enzyme adenylation" evidence="19">
        <location>
            <begin position="43"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="mRNA capping enzyme C-terminal" evidence="20">
        <location>
            <begin position="249"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="415"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="445"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="active site" description="N6-GMP-lysine intermediate" evidence="17">
        <location>
            <position position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00044624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR036959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR036959-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF01331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF03919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="CAR30187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="468" mass="53802" checksum="69EDD14E53D99B92" modified="2009-07-28" version="1">MDNRAAPEIPGIRQPANVTQDIRLLVCKLLNSPKPAKTFPGSQPISFHHSDIEEKLLQQDYYVCEKTDGLRGLMLIVINPVTKEQGCFIIDRENNYYQVNGFRFPRLPKQSRKELLETFQDGTLIDGELVLQTNPVTKVKELRYLMFDCLAINGRCIVQSPTSSRLAHLGKEFFKPYYDLRSLYPAQCATFPFKISMKHMSFSVDLVKVANSLDKLPHVSDGLIFTPVTTPYVVGGKDSLLLKWKPETENTVDFKMILDIPKAEDTSLSKNDPNRFFYNYGVKPSFHLYIWQGGADVNARLHDFEQPFSKKELEVLDRTYKKFADLDVSDSQWTELKSLGEPLNGRIVECAKDQETGVWRMLRFRDDKLNGNYVSVVQKVLESISDSVKIEDLEEATAQMKSNWEKRQIEKKRGFQEVQDARRVTQPAPPTQPAPQNTEQPKYIDDDDEELWSDDDDESDTEAKRQKL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-05-31" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2G4</accession>
    <name>LCL3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName>Probable endonuclease LCL3</fullName>
            <ecNumber>3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">LCL3</name>
        <name type="ordered locus">KLTH0H04752g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the LCL3 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556087.1">
        <property type="nucleotide sequence ID" value="XM_002556041.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2G4"/>
    <dbReference type="FunCoup" id="C5E2G4">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2G4"/>
    <dbReference type="GeneID" id="8294401"/>
    <dbReference type="KEGG" id="lth:KLTH0H04752g"/>
    <dbReference type="eggNOG" id="ENOG502S1U4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046484_0_1_1"/>
    <dbReference type="InParanoid" id="C5E2G4"/>
    <dbReference type="OMA" id="IYHTPGG"/>
    <dbReference type="OrthoDB" id="430293at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035437">
        <property type="entry name" value="SNase_OB-fold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016071">
        <property type="entry name" value="Staphylococal_nuclease_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12302">
        <property type="entry name" value="EBNA2 BINDING PROTEIN P100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12302:SF3">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00565">
        <property type="entry name" value="SNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00318">
        <property type="entry name" value="SNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50199">
        <property type="entry name" value="Staphylococcal nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50830">
        <property type="entry name" value="TNASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0106">Calcium</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0496">Mitochondrion</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000408664" description="Probable endonuclease LCL3">
        <location>
            <begin position="1"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="15"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="TNase-like" evidence="3">
        <location>
            <begin position="58"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="143"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="151"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="191"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="266" mass="30650" checksum="8175E831C7AA309A" modified="2009-07-28" version="1">MSEDRAVRLSSQQILYTKAAILSAALTGSILTSYALFNRYLKQYTRVTQIPLSAFRKNWLYGKVTSVGDGDNFHLFHTPGGILGGWGWARSAPKLDKVNNKRSDSLKRLFFPRFLRRQKEQEKFQSLNVPFKGRRNLPTLSIRLCGVDAPERAHFGNAAQPFSEEALIWLRHTLIGKFVWIKPLSLDQYGRCVAKVKFWSWTGWKNVSLEMVKNGIAVVYEGKTGAEFDDERDIYIFHEQVARKERRGLWSLKKFETPGNYKKRVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2V0</accession>
    <name>C5E2V0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0H07898p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0H07898g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the actin-binding proteins ADF family. GMF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556223.1">
        <property type="nucleotide sequence ID" value="XM_002556177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2V0"/>
    <dbReference type="FunCoup" id="C5E2V0">
        <property type="interactions" value="115"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2V0"/>
    <dbReference type="GeneID" id="8294541"/>
    <dbReference type="KEGG" id="lth:KLTH0H07898g"/>
    <dbReference type="eggNOG" id="KOG1736">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087056_0_0_1"/>
    <dbReference type="InParanoid" id="C5E2V0"/>
    <dbReference type="OMA" id="EWKMLYA"/>
    <dbReference type="OrthoDB" id="3919494at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071933">
        <property type="term" value="F:Arp2/3 complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071846">
        <property type="term" value="P:actin filament debranching"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034316">
        <property type="term" value="P:negative regulation of Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11283">
        <property type="entry name" value="ADF_GMF-beta_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002108">
        <property type="entry name" value="ADF-H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011171">
        <property type="entry name" value="GMF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11249:SF2">
        <property type="entry name" value="GLIA MATURATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11249">
        <property type="entry name" value="GLIAL FACTOR NATURATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00241">
        <property type="entry name" value="Cofilin_ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001788">
        <property type="entry name" value="GMF-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00102">
        <property type="entry name" value="ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51263">
        <property type="entry name" value="ADF_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ADF-H" evidence="4">
        <location>
            <begin position="6"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR001788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR30361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="146" mass="16510" checksum="58DBE85207E8DFCF" modified="2009-07-28" version="1">MSSLYHISTETKNTIRKYRTATARSESLQALVIKIEPKPSCEIIIDDDEELREASSLEELGEELPDNAPRYVLLCYPMTTADGRKQTPLVLLYWKPATVVSQEWKMLYAGALEMVRSECGVSKVIEVATGLEDEDEIEELKEQIQG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-03-08" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>C5E368</accession>
    <name>COA3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName>Cytochrome c oxidase assembly factor 3, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">COA3</name>
        <name type="ordered locus">KLTH0H10802g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for assembly of cytochrome c oxidase (complex IV).</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of 250-400 kDa complexes called cytochrome oxidase assembly intermediates or COA complexes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Mitochondrion inner membrane</location>
            <topology>Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the COA3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556341.1">
        <property type="nucleotide sequence ID" value="XM_002556295.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E368"/>
    <dbReference type="SMR" id="C5E368"/>
    <dbReference type="FunCoup" id="C5E368">
        <property type="interactions" value="38"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E368"/>
    <dbReference type="GeneID" id="8294672"/>
    <dbReference type="KEGG" id="lth:KLTH0H10802g"/>
    <dbReference type="eggNOG" id="ENOG502S440">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_153999_0_0_1"/>
    <dbReference type="InParanoid" id="C5E368"/>
    <dbReference type="OMA" id="WKMTPAM"/>
    <dbReference type="OrthoDB" id="10018333at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041752">
        <property type="entry name" value="Coa3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15642:SF3">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 3 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15642">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0496">Mitochondrion</keyword>
    <keyword id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000405442" description="Cytochrome c oxidase assembly factor 3, mitochondrial">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="topological domain" description="Mitochondrial matrix" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="31"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="topological domain" description="Mitochondrial intermembrane" evidence="1">
        <location>
            <begin position="54"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="91" mass="10734" checksum="853102FB9F377214" modified="2009-07-28" version="1">MAFEPSRYQDPRTWKMTPAMIRARRPFFKKNLLGLGILVSVTGGIYVYTHRFLNRDNDFADVPIPPIDPKELEQLKKEYEQHKRDVAARDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>C5E397</accession>
    <name>C5E397_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0H11506p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0H11506g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556370.1">
        <property type="nucleotide sequence ID" value="XM_002556324.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E397"/>
    <dbReference type="FunCoup" id="C5E397">
        <property type="interactions" value="76"/>
    </dbReference>
    <dbReference type="GeneID" id="8294703"/>
    <dbReference type="KEGG" id="lth:KLTH0H11506g"/>
    <dbReference type="eggNOG" id="ENOG502S0IJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060982_0_0_1"/>
    <dbReference type="InParanoid" id="C5E397"/>
    <dbReference type="OMA" id="DARMYST"/>
    <dbReference type="OrthoDB" id="4068767at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="105"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="222"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="263"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="284"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR30508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="304" mass="33511" checksum="8C7598CFE263819A" modified="2009-07-28" version="1">MERSSTPSLVGVSVRSINDSNNPDFKGNRGPSVAEARSSPQLLEKIHSSTQLNKLSSAYTTVDELNREGALLTDDNDVDLDKVVQGDTEGASGRRKVLLRRKKNNSSSSKMPSSSSLSVSSMMSPSRSRSSLVTSTDPVHDHASEGEARAGAATADNEDRIRNSYGEFIPNRSHRPHLAGGESYQSTQNDSTGEDADAERPGRSSKREDASRGYLRSLSRSLSRDPQKKAHDQEQEDARMYSTNNYRISQLDLENAPHVIKEEIEEEQDQGALMDDEGNEEYSEPLRESASKQLDNDKLQEGDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3A4</accession>
    <name>C5E3A4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0H11682p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0H11682g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556377.1">
        <property type="nucleotide sequence ID" value="XM_002556331.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3A4"/>
    <dbReference type="GeneID" id="8294711"/>
    <dbReference type="KEGG" id="lth:KLTH0H11682g"/>
    <dbReference type="eggNOG" id="ENOG502S5VC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_298094_0_0_1"/>
    <dbReference type="InParanoid" id="C5E3A4"/>
    <dbReference type="OrthoDB" id="4036511at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR30515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1009" mass="113682" checksum="021AD4D3BAB417E7" modified="2009-07-28" version="1">MFVDYSSQNKFMSINYKFADKYLEMLHIEKLSSPENRLECLKVRTEGLGIKIIQNLEINDFAGDFNWIVYLYGLVLSKEQMLECGETLLSCPTLYMVEFALQGCRIEDAFSEESTLSSQLKLAIKCAYYEFIGPCEKIIYSLKTHFLSLRPRYDLPFNKFGYFQKVLYNEMFTDQAGNYKIHAGLLIMPSKFPSSYRILISEEGYPKSNIKVRFHKWSGYRAKSNLNRLEMNDVKPSCIENKVISVWKQGHSSELFGHYSYMLRLGKLPSNEEPPSETTVVMKDIKARSAKRTEKLRQFEDFVTSGNSWSSQKGQGVSSTYKSADLSFEGCQNSFNSKTDPSNEVLEFKARPSKRSRILPEGVMRSDSTACNTSIQGHIVSSRCGEQSPTSFIKENCTPVPIEPPNTAGDTNAGPHEIMRKTSSGVYENNKINSSLSNKYSRPKGQQTCVLAQCEKPLLPKKRLLVKSGTAGYREMTELEKDIYHLLEIRQTPKGVRGSAPLPSRSDKISAEKAKRFQQVRQELMENEEEFLFSSKKAGPPLSGDGVIDRHLKIAASTSRPQNAINPNLVKSLKPVSEIVAIIEKAEKVVAGQDLVQQLEGISQELKCSKSLESLSSSTLGKSFDNYSTARCYSSPGVPVNERGKNKTIAPAEKAACPPGENPLPNASIVQNRVTVPLDKALSSNDFYKFMNSSETLDSEQSVIIRRHLFSSEHEVQGQVYPSPEGQKENNASLSGEFLIEANSVSSSYDSSEIPEKCCVRSWQHITEAKNSIEAKKRPERSSCEAVFSYEPSDSSPERLKDLSAGEFSNFRNRNSALLTTSKDSGSYSASQPCSPTFSDAASLATSHTRPLDLKSKCASECSNNALCAKNRYCSIESGYVATELPVFKINENDPDLLYRVYMADPQNCLGQPEVIFRKPFEQRSNGLTQKLRKFMSSHSSSRKNTKSEPTYQEMYPALSNIRFRDFLRFKSRKLREDVRYFSHVAKLCVTDFKERPITAERELYEQFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3F1</accession>
    <name>C5E3F1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0H12914p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0H12914g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556424.1">
        <property type="nucleotide sequence ID" value="XM_002556378.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3F1"/>
    <dbReference type="GeneID" id="8294767"/>
    <dbReference type="KEGG" id="lth:KLTH0H12914g"/>
    <dbReference type="eggNOG" id="ENOG502S4KN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056726_0_0_1"/>
    <dbReference type="InParanoid" id="C5E3F1"/>
    <dbReference type="OMA" id="DYSHYTH"/>
    <dbReference type="OrthoDB" id="4030310at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.320.10">
        <property type="entry name" value="YwqG-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015315">
        <property type="entry name" value="DUF1963"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035948">
        <property type="entry name" value="YwqG-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36436">
        <property type="entry name" value="SLL5081 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36436:SF6">
        <property type="entry name" value="SLL5081 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09234">
        <property type="entry name" value="DUF1963"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103032">
        <property type="entry name" value="Hypothetical protein YwqG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR30562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="264" mass="31067" checksum="EA5FF8DCB66B0550" modified="2009-07-28" version="1">MTKFLLPPEVEEYRAVFEQTQVESIRLEAKKGHTGPYDSKFGGEPYFPLGFQYPLDEDGRPMKLLAQINFEDMPTLENYPQYGILQFYLTTNNETYGLDYEPPHQQRYFRILYFEEVLGDEEKIVSDFSFLPEKKPNDEDFPIESEAKVTFVKQTEIISPFDYRFDKTVTVPSTVWNVDVQCKYEEMQDAFHHKIGGYGAFVQQDPREESRIDYSHYTHLLFQIASEDEIDCCWGDGGVANFFITREDLKNKCFDHVLYNWDCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3H6</accession>
    <name>C5E3H6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 18">Phosphoglycerate kinase</fullName>
            <ecNumber evidence="6 18">2.7.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ordered locus">KLTH0H13640g</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes one of the two ATP producing reactions in the glycolytic pathway via the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. Both L- and D- forms of purine and pyrimidine nucleotides can be used as substrates, but the activity is much lower on pyrimidines. Negatively regulates the biosynthesis of acetyl-CoA from pyruvate in the mitochondrion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 18">
            <text>(2R)-3-phosphoglycerate + ATP = (2R)-3-phospho-glyceroyl phosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:14801"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 19">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 18">Belongs to the phosphoglycerate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.3" evidence="6 18"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556449.1">
        <property type="nucleotide sequence ID" value="XM_002556403.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3H6"/>
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        <property type="interactions" value="840"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3H6"/>
    <dbReference type="GeneID" id="8294810"/>
    <dbReference type="KEGG" id="lth:KLTH0H13640g"/>
    <dbReference type="eggNOG" id="KOG1367">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025427_0_0_1"/>
    <dbReference type="InParanoid" id="C5E3H6"/>
    <dbReference type="OMA" id="DMIFDIG"/>
    <dbReference type="OrthoDB" id="275353at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:phosphoglycerate kinase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="P:gluconeogenesis"/>
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    </dbReference>
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        <property type="term" value="P:glycolytic process"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00318">
        <property type="entry name" value="Phosphoglycerate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphoglycerate kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1260">
        <property type="entry name" value="Phosphoglycerate kinase, N-terminal domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00145">
        <property type="entry name" value="Phosphoglyc_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001576">
        <property type="entry name" value="Phosphoglycerate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015911">
        <property type="entry name" value="Phosphoglycerate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015824">
        <property type="entry name" value="Phosphoglycerate_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036043">
        <property type="entry name" value="Phosphoglycerate_kinase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11406">
        <property type="entry name" value="PHOSPHOGLYCERATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11406:SF0">
        <property type="entry name" value="PHOSPHOGLYCERATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00162">
        <property type="entry name" value="PGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000724">
        <property type="entry name" value="Pgk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00477">
        <property type="entry name" value="PHGLYCKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53748">
        <property type="entry name" value="Phosphoglycerate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00111">
        <property type="entry name" value="PGLYCERATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="11 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 18" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="24"/>
            <end position="26"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>(2R)-3-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="63"/>
            <end position="66"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>(2R)-3-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>(2R)-3-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="342"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="371"/>
            <end position="374"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013061"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00016471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR000724-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR000724-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU000696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CAR30587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="416" mass="44591" checksum="AB33F229D5DB624C" modified="2009-07-28" version="1">MSLSSKLTVKDLNVKDKRVFIRVDFNVPLDGQTITSNQRIVAALPTIKYVLEQKPKVVVLASHLGRPNGERVEKYSLKPVAAELQKLLGKDVTFLNDCVGPEVTKAVNSAAPGSVILLENLRFHIEEEGSRKVDGKKVKADKADVEKFRKELCSLADVYVNDAFGTAHRAHSSIVGFELEQRAAGFLLSKELEYFGKALENPARPFLAILGGAKVADKIQLIDNLLDKVDSIIIGGGMAFTFKKVLEGTEIGNSIFDKAGAEIVPKLAEKAKQKNVQIVLPIDFVCGDSFSADAQVKNVTDKEGVPQGWEGLDCGPESRKLFSAAVAKAKTIVWNGPPGVFEFEKFAEGTQALLKDAVKASESGATVIIGGGDTATVAKKYGVVDKISHVSTGGGASLELLEGKELPGVTFLSNKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3M7</accession>
    <name>C5E3M7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Terpene cyclase/mutase family member</fullName>
            <ecNumber evidence="14">5.4.99.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">KLTH0H14850g</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(S)-2,3-epoxysqualene = lanosterol</text>
            <dbReference type="Rhea" id="RHEA:14621"/>
            <dbReference type="ChEBI" id="CHEBI:15441"/>
            <dbReference type="ChEBI" id="CHEBI:16521"/>
            <dbReference type="EC" id="5.4.99.7"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:14622"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="13">Terpene metabolism; lanosterol biosynthesis; lanosterol from farnesyl diphosphate: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lipid droplet</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the terpene cyclase/mutase family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.-" evidence="14"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556500.1">
        <property type="nucleotide sequence ID" value="XM_002556454.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3M7"/>
    <dbReference type="FunCoup" id="C5E3M7">
        <property type="interactions" value="133"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3M7"/>
    <dbReference type="GeneID" id="8294864"/>
    <dbReference type="KEGG" id="lth:KLTH0H14850g"/>
    <dbReference type="eggNOG" id="KOG0497">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009074_2_1_1"/>
    <dbReference type="InParanoid" id="C5E3M7"/>
    <dbReference type="OMA" id="CWARQTI"/>
    <dbReference type="OrthoDB" id="21502at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000250">
        <property type="term" value="F:lanosterol synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016104">
        <property type="term" value="P:triterpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02892">
        <property type="entry name" value="SQCY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000003">
        <property type="entry name" value="Terpene cyclase/mutase family member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000027">
        <property type="entry name" value="Terpene cyclase/mutase family member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.120.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032696">
        <property type="entry name" value="SQ_cyclase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032697">
        <property type="entry name" value="SQ_cyclase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018333">
        <property type="entry name" value="Squalene_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002365">
        <property type="entry name" value="Terpene_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01787">
        <property type="entry name" value="squalene_cyclas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11764:SF20">
        <property type="entry name" value="LANOSTEROL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11764">
        <property type="entry name" value="TERPENE CYCLASE/MUTASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13243">
        <property type="entry name" value="SQHop_cyclase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13249">
        <property type="entry name" value="SQHop_cyclase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01016">
        <property type="entry name" value="Prenyltransferase_Like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01074">
        <property type="entry name" value="TERPENE_SYNTHASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11 14" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0752">Steroid biosynthesis</keyword>
    <feature type="domain" description="Squalene cyclase N-terminal" evidence="16">
        <location>
            <begin position="87"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="Squalene cyclase C-terminal" evidence="15">
        <location>
            <begin position="387"/>
            <end position="721"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00051240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00060682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU362003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF13243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF13249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAR30638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="726" mass="82845" checksum="6EF2A32DE72838A7" modified="2009-07-28" version="1">MKLYADELGLPTTDKTLWRLRTDELGSEKWEYLSEEEAKADPQSSYVKYLLQNPDFEAPEPLRSSKCDEFTAGDAAFNGAQFFSLLQEDTGIFPCQYKGPMFLTIGYVATLYCARIPVPEHIKIEIIRYIVNSAHPVDGGWGLHEVDKSTTFGTAINYVILRLLGLEADHIVCQRARKTLHRLGGAIGAPHWGKAWLSILNLYRWEGVNPAPPELWLLPYALPIHPGRWWVHTRAVYQALGYLSAAKYCCELTPLLQEIRKEIYVKPFESIDFSKHKNTVGGVDLYYPHTKLLNIANVALTLYENYLKPNWFLKYSNRKVYELIKKEIKNTDYLCIAPVSMAFCAVVTLIEEGRDSYEFKRFVDGFQEVLFMGPQGMTVMGTNGVQVWDCAFAIQYYFVAGLAELPEFRDTIEKAYKFLCRSQFDTECEPGSFRDKRVGAWPFSTKTQGYTVSDCTAEAVKAIIMVRNSPKFTYLRGEISDERLYKAVDILLGLQNTGSFEYGSFATYEKIKATTALEKLNPAEVFGNIMVEFPYVECTDSSVLGLTYFAKYYNYRKDEITKAIEIAIQYIKRVQNPDGSWYGCWGVCFTYAGMFALEALYTVGENYENSEVVRKGCDFFASRQRSDGGWSESMKSSELHTYVEADNSQVVQTAWALIGLLLAKYPNRDVIDRGIALLKKRQLPSGEWKFEDVEGVFNHSCAIEYPSYKFLFPIKALGLYSKRYES</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBD4</accession>
    <name>C5DBD4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0A01650p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0A01650g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551533.1">
        <property type="nucleotide sequence ID" value="XM_002551487.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBD4"/>
    <dbReference type="GeneID" id="8290326"/>
    <dbReference type="KEGG" id="lth:KLTH0A01650g"/>
    <dbReference type="HOGENOM" id="CLU_2061917_0_0_1"/>
    <dbReference type="InParanoid" id="C5DBD4"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="11"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="119" mass="12817" checksum="8C71AD5EF81BDD56" modified="2009-07-28" version="1">MSESLEHKAEQAQQAAAVNAEHAQAKVAEVGDNAERSAQETQEKAVAAAEEQAQEAKKEANKVEESAKKEAEGVKESAQDKVDEVKKTTAAEKSETAAAEGEKVSPWKRFVAKLKKLLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBE0</accession>
    <name>C5DBE0_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Chitin synthase</fullName>
            <ecNumber evidence="2 7">2.4.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0A01826g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Polymerizes chitin, a structural polymer of the cell wall and septum, by transferring the sugar moiety of UDP-GlcNAc to the non-reducing end of the growing chitin polymer.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>[(1-&gt;4)-N-acetyl-beta-D-glucosaminyl](n) + UDP-N-acetyl-alpha-D-glucosamine = [(1-&gt;4)-N-acetyl-beta-D-glucosaminyl](n+1) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16637"/>
            <dbReference type="Rhea" id="RHEA-COMP:9593"/>
            <dbReference type="Rhea" id="RHEA-COMP:9595"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17029"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="EC" id="2.4.1.16"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the chitin synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.16" evidence="2 7"/>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551539.1">
        <property type="nucleotide sequence ID" value="XM_002551493.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBE0"/>
    <dbReference type="FunCoup" id="C5DBE0">
        <property type="interactions" value="117"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBE0"/>
    <dbReference type="GeneID" id="8290334"/>
    <dbReference type="KEGG" id="lth:KLTH0A01826g"/>
    <dbReference type="eggNOG" id="KOG2571">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004760_3_2_1"/>
    <dbReference type="InParanoid" id="C5DBE0"/>
    <dbReference type="OMA" id="TTMRETE"/>
    <dbReference type="OrthoDB" id="26569at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030428">
        <property type="term" value="C:cell septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004100">
        <property type="term" value="F:chitin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006031">
        <property type="term" value="P:chitin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04190">
        <property type="entry name" value="Chitin_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004835">
        <property type="entry name" value="Chitin_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013616">
        <property type="entry name" value="Chitin_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914">
        <property type="entry name" value="CHITIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914:SF38">
        <property type="entry name" value="CHITIN SYNTHASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01644">
        <property type="entry name" value="Chitin_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08407">
        <property type="entry name" value="Chitin_synth_1N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="3 7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="609"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="642"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="673"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="709"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="741"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="842"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="874"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin synthase N-terminal" evidence="9">
        <location>
            <begin position="199"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="771"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="30"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="52"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR21097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="930" mass="106169" checksum="457BE9DE1ADB4740" modified="2009-07-28" version="1">MDYNPFVVDTNNASPVRSPQRAARRGESPWRSQYSANSSNENDLSTAFQGFPQAAQVQPQSPSKAAAKYSPQRRNIFARDSAPSSPLRQDRYTANMRESPHRNARAVIEIDEASDDDEEQDLYDIYAQQADGSPSRAASRATRSTYSFESYRKSLDDDSTIYSGDTFEQTQFQVNHPQKNYIRRGKSEVRRLPAPTSKKNILKLDNPIPRGLLEILPRRDSLEFTEMRYTACTSEPDNYVNEGYSLRFAEMNRECQIAICVTMYNEDKFALSSTLHSIMRNITHMCKRKRSHVWGPQGWKKIQVIIVSDGRNNINKGSLDYLSALGVYQEDMAKSTVNGEPVKAHIFELTTQVSIDSHLDYVSKDIVPVQVVFCLKEENQKKINSHRWLFNAFCPILDPTVVVLVDVGTKLNDSAIYHLWKAFDRDSNVAGAAGQIKTMKGKWGRKLLNPLVASQNFEYKMSNILDKPLESVFGYISVLPGALSAYRYRALKNHADGTGPLNSYFLGETQEGRQHDVFTANMYLAEDRILCWELVAKRDAKWVLKYVKEATGETDVPEEPPEFISQRRRWLNGAMFAALYAQLHFTQIWKTKHSATRKIFFHLEFFYQFVQMLFSWFSISNFFLTFYFLAGSMNKLMKHGDVLFTFFKYLVICDLAALFIISMGNRPQGANHLFIISMIILSICSTYALVCGLVFSIKTLQNPGESSMVFVNIVVSLLSTYGLYAFTSIMYLDPWHLITSFVQYLLMLPSFICTLQIFAFCNTHDVSWGTKGSTQDVKPKSEATVKQGPNGQQIVEGTEWPQEVDKKYAEIKSRLKEQEIIEPKIDEAQKNNDYYRDIRTRIVMLWMLSNLVLMMIVTQVYGPEDTAKNKYLTFILWSVAILALFRALGSMAFLFLKSLRAIVSFKHKAEDSGSLNIPSAKNLFSKPSKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBF7</accession>
    <name>C5DBF7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="14">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ordered locus">KLTH0A02244g</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="14">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the DEAD box helicase family. DDX55/SPB4 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="14"/>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551556.1">
        <property type="nucleotide sequence ID" value="XM_002551510.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBF7"/>
    <dbReference type="FunCoup" id="C5DBF7">
        <property type="interactions" value="1277"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBF7"/>
    <dbReference type="GeneID" id="8290353"/>
    <dbReference type="KEGG" id="lth:KLTH0A02244g"/>
    <dbReference type="eggNOG" id="KOG0345">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_26_4_1"/>
    <dbReference type="InParanoid" id="C5DBF7"/>
    <dbReference type="OMA" id="AYKEHEC"/>
    <dbReference type="OrthoDB" id="7396459at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17960">
        <property type="entry name" value="DEADc_DDX55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056330">
        <property type="entry name" value="CTT_SPB4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025313">
        <property type="entry name" value="SPB4-like_CTE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13959">
        <property type="entry name" value="CTE_SPB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23681">
        <property type="entry name" value="CTT_SPB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01178">
        <property type="entry name" value="DUF4217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 13" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="8 14" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="18">
        <location>
            <begin position="7"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="16">
        <location>
            <begin position="38"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="17">
        <location>
            <begin position="248"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="526"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="12">
        <location>
            <begin position="7"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="537"/>
            <end position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAR21114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="598" mass="67504" checksum="8844F18B6A07A595" modified="2009-07-28" version="1">MARSLSWDNLKYELQPWIRTAIDAMGFEIMTPVQASTIPMFSGNKDVVVESVTGSGKTIAFTIPILEKVVKEVLSGSPLKRGHFYSLIISPTRELSKQIQDVVEAFLAYYPSESCPIKSQLIVGTSSCSVRDDVSKFLENAPQILIGTPGRVLDFMKASTVKTSSCGVVVLDEADRLLDVSFEKDVESILGMLPKQRRTGLFSATISSAGNQIYRTGMRNPVKIAVKSKVQNPESLQINYIVVKPEEKLHHLLNIINNMRYRKCIVYFPTCQAVTYFYSFLKHLSDISKTRDDLEVYSLHGKLQTNSRIGTLAKFSTSLNIAVLLTTDVAARGIDIPEVDLVLQFDPPTNAEMFLHRCGRTGRANRAGKAITFINCGREEDYIDFLGVKNISLGELEISFRPVDELGVIFKSWVLQDRARFEHGIKAYAAFIRYYSKHSASSIFRLQSLDYVGLAKLYGLIRLPRMPEITKYMSADATPSEGWLIDPPLDLDHFSYLNKQMERARQEELKKTKEISDKKKLKSELKKKNAAWSNKTTSKETRKERKVKLAEKRKAIEQKIAEAGSEDESDGEEDWKDLVRRNKKAKTCSQIQGDFSDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBI4</accession>
    <name>C5DBI4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DnaJ homolog 1, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0A02882g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551583.1">
        <property type="nucleotide sequence ID" value="XM_002551537.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBI4"/>
    <dbReference type="FunCoup" id="C5DBI4">
        <property type="interactions" value="1013"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBI4"/>
    <dbReference type="GeneID" id="8290382"/>
    <dbReference type="KEGG" id="lth:KLTH0A02882g"/>
    <dbReference type="eggNOG" id="KOG0715">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017633_0_3_1"/>
    <dbReference type="InParanoid" id="C5DBI4"/>
    <dbReference type="OMA" id="MATDYYA"/>
    <dbReference type="OrthoDB" id="10256793at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072655">
        <property type="term" value="P:establishment of protein localization to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10747">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10719">
        <property type="entry name" value="DnaJ_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000005">
        <property type="entry name" value="Chaperone protein dnaJ 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.230.10:FF:000001">
        <property type="entry name" value="DnaJ subfamily A member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000053">
        <property type="entry name" value="Putative Mitochondrial DnaJ chaperone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.230.10">
        <property type="entry name" value="Heat shock protein DnaJ, cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.20">
        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01152">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012724">
        <property type="entry name" value="DnaJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001305">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43096">
        <property type="entry name" value="DNAJ HOMOLOG 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43096:SF52">
        <property type="entry name" value="DNAJ HOMOLOG 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00684">
        <property type="entry name" value="DnaJ_CXXCXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="DnaJ/Hsp40 cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51188">
        <property type="entry name" value="ZF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="5 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="J" evidence="12">
        <location>
            <begin position="40"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="CR-type" evidence="13">
        <location>
            <begin position="193"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CR-type" evidence="10">
        <location>
            <begin position="193"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="432"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="434"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00072890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR21141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="473" mass="50119" checksum="151A646AD4F92B0F" modified="2009-07-28" version="1">MYRRTLLRLSNPRALAPRLATGPSARLFHASTRLLDELKDPYATLGVSKSASGSEIKKAYYKLAKKYHPDINKAEDAQKKFHDLQNAYEILSDDSKRQQWDQFGPAAFSQGGAGAPGGGAGGAGAEGFGGFGGFGGAGFGGINFEDLFGAAFGGGAGAGGAGARSRGSMFREYQGSPIELTHRISFKDSVFGTKNVNLKFSAYDPCGTCDGSGLKPGASRTTCPGCSGTGTRVHIRAGFQMASTCNQCGGEGSVVRDSDTCATCHGEGASLNKDHAIKVDLPHGLQDGDVIRISGQGSYPNIAVDPAMKDTVRLSRGDVLLRIRVDKDPRFQIKNKYDIWLTQDIPITTAALGGIVTVPTVDGEQVRLKVAPGTQHNDVVSISQKGVPRGAFGSRGDMKVQYKISIKKPQSQAERCLWEALADVTGDTLARRSVLSSQPQQSSHTDNPDAPGALGKLENFISNTFKKIKGDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBJ6</accession>
    <name>C5DBJ6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0A03168p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0A03168g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551595.1">
        <property type="nucleotide sequence ID" value="XM_002551549.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBJ6"/>
    <dbReference type="GeneID" id="8290395"/>
    <dbReference type="KEGG" id="lth:KLTH0A03168g"/>
    <dbReference type="eggNOG" id="ENOG502RY18">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048298_0_0_1"/>
    <dbReference type="InParanoid" id="C5DBJ6"/>
    <dbReference type="OMA" id="PHADYFD"/>
    <dbReference type="OrthoDB" id="4069959at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060963">
        <property type="term" value="P:positive regulation of ribosomal protein gene transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052146">
        <property type="entry name" value="HOT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022210">
        <property type="entry name" value="TF_GCR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37784:SF2">
        <property type="entry name" value="HIGH-OSMOLARITY-INDUCED TRANSCRIPTION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37784">
        <property type="entry name" value="PROTEIN MSN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12550">
        <property type="entry name" value="GCR1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription activator GCR1-like" evidence="2">
        <location>
            <begin position="304"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="104"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR21153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="388" mass="43729" checksum="4659DC343D717F15" modified="2009-07-28" version="1">METDKVSSVSERLQLLMDVNHEQHDRPSGSLLTHLISTLDQETTAPPTLAEDAPSLQEDAAERTFTASSYYTSFVPARPHADYFDFDTEYNEKAKDLYGDVEEAEAAAGRSDTGVERRLETMEESTLEASPVGLPTDDPSDDADQNVQMILDKLDTFKREFRHLIKRMDEASLVSRISGSATLPDMTARPRVMNTLVGEIIDTLQSFVPQPSRKRSRCDSDVGIAEKDEESSLLDAVAKETLEHDPCYGVPNFGVVLIKSPTTVSQLWNEYTKLPSEWALPDWFDVASQQSSGANNARRPAILLKRRTSIRDLERIYGSSWRNGDKNFSRQVNRRKKIWHAIEEGLESGIPLEECFRLLESYVKERGKGLSWYYNGVPFKLAELRGGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBT6</accession>
    <name>C5DBT6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0A05236p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0A05236g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0057 (PMP3) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551685.1">
        <property type="nucleotide sequence ID" value="XM_002551639.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBT6"/>
    <dbReference type="FunCoup" id="C5DBT6">
        <property type="interactions" value="354"/>
    </dbReference>
    <dbReference type="GeneID" id="8290489"/>
    <dbReference type="KEGG" id="lth:KLTH0A05236g"/>
    <dbReference type="eggNOG" id="KOG1773">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107649_0_2_1"/>
    <dbReference type="InParanoid" id="C5DBT6"/>
    <dbReference type="OMA" id="TIYYVYR"/>
    <dbReference type="OrthoDB" id="2802411at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000612">
        <property type="entry name" value="PMP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21659">
        <property type="entry name" value="HYDROPHOBIC PROTEIN RCI2 LOW TEMPERATURE AND SALT RESPONSIVE PROTEIN LTI6 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21659:SF114">
        <property type="entry name" value="PROTEIN SNA4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01679">
        <property type="entry name" value="Pmp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="13"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="78"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="94"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="122"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR21243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="131" mass="14562" checksum="11C389621CF78A8A" modified="2009-07-28" version="1">MCFYCHCFTVSDLILYIVGFFIPPVAVLLRSGCCSSDLLLNILLTMLGVVPGMLHAFYYITMTSPLRREENRYYYQSGWDDGRRSRHQGSPGASEREHVVREEPSAVTPLMPNTGVGKPASGAPPPYTESV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DC48</accession>
    <name>C5DC48_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0A07766p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0A07766g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551797.1">
        <property type="nucleotide sequence ID" value="XM_002551751.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DC48"/>
    <dbReference type="STRING" id="559295.C5DC48"/>
    <dbReference type="GeneID" id="8290604"/>
    <dbReference type="KEGG" id="lth:KLTH0A07766g"/>
    <dbReference type="eggNOG" id="ENOG502QW7R">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008109_1_0_1"/>
    <dbReference type="InParanoid" id="C5DC48"/>
    <dbReference type="OMA" id="TQMAMEY"/>
    <dbReference type="OrthoDB" id="5229455at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF43">
        <property type="entry name" value="FINGER DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G01850)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="3">
        <location>
            <begin position="11"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR21355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="605" mass="67738" checksum="683904812D55F2FA" modified="2009-07-28" version="1">MRVTSKRSRGGCRNCKRSKIKCDERKPKCGHCIRKGKDDCTYSLALRWGNNPSRSLKTRKGDSKTTLLRDAINVTSSRDPHSSSATVNFPSLVPAEAGIEQLLWPTGHELLSSPGVSYRPQSFKESFLQAPLSPFSFDSPFSGHSERPINSGLAEPGTLELSHPSPSILSNSPYFSELFGFFLRETSRLLVPVPSYAYRSNPFHTLIPQMAMQSPALMGLILAFGANHRNKMTAYQEQCSFPSLDEKSSAKNIDGLLADELLSSTISQLLVKLMNAEERRSDTTLATILMLAGFDIFFCDTRNKWRAHILGAKKLLMDRLNSKSGTKTMIFKIPSANNVLDTETFLVHWFSYLNIIASLSSASPKNSTEASRGVGYEFCYEDNSAHTHELRLQLRDIDYFTGVDTKILSLLAGVSCLIEEKKVIEQPFGIHNLVLKALELDHEIRSYLEASEAARDRVESQLLGEDASSDTKLRFQTYKTMRATNLIFGLTGVLQLKRRVIGIPHNSKILKDLLAEVTALIDKEIPHNSSAASCITFCLFCCGCELIDDNLACHRETYTEHIEALLRKGVTSALQAQSIMKECWREKKCWWEILEEKNLDLAFAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>C5DC94</accession>
    <name>C5DC94_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="16">KLTH0B01188p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">KLTH0B01188g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>pyruvate + H(+) = acetaldehyde + CO2</text>
            <dbReference type="Rhea" id="RHEA:45484"/>
            <dbReference type="ChEBI" id="CHEBI:15343"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="11">Binds 1 Mg(2+) per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the TPP enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551843.1">
        <property type="nucleotide sequence ID" value="XM_002551797.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DC94"/>
    <dbReference type="FunCoup" id="C5DC94">
        <property type="interactions" value="1131"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DC94"/>
    <dbReference type="GeneID" id="8290669"/>
    <dbReference type="KEGG" id="lth:KLTH0B01188g"/>
    <dbReference type="eggNOG" id="KOG1184">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013748_0_2_1"/>
    <dbReference type="InParanoid" id="C5DC94"/>
    <dbReference type="OMA" id="IHGPEQR"/>
    <dbReference type="OrthoDB" id="3970464at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004737">
        <property type="term" value="F:pyruvate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000949">
        <property type="term" value="P:aromatic amino acid family catabolic process to alcohol via Ehrlich pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02005">
        <property type="entry name" value="TPP_PDC_IPDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07038">
        <property type="entry name" value="TPP_PYR_PDC_IPDC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1220:FF:000018">
        <property type="entry name" value="Pyruvate decarboxylase isozyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000019">
        <property type="entry name" value="Pyruvate decarboxylase isozyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000024">
        <property type="entry name" value="Pyruvate decarboxylase isozyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012110">
        <property type="entry name" value="PDC/IPDC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047214">
        <property type="entry name" value="TPP_PDC_IPDC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047213">
        <property type="entry name" value="TPP_PYR_PDC_IPDC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43452">
        <property type="entry name" value="PYRUVATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43452:SF30">
        <property type="entry name" value="PYRUVATE DECARBOXYLASE ISOZYME 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036565">
        <property type="entry name" value="Pyruvt_ip_decrb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="6 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme N-terminal TPP-binding" evidence="15">
        <location>
            <begin position="34"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme central" evidence="13">
        <location>
            <begin position="230"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="14">
        <location>
            <begin position="428"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <label>1</label>
            <note>substrate; ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <label>1</label>
            <note>substrate; ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <label>2</label>
            <note>allosteric activator</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="500"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="502"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="506"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <label>1</label>
            <note>substrate; ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR036565-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR036565-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAR21405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="593" mass="65284" checksum="DB95803C643A7F43" modified="2009-07-28" version="1">MSDLILYCDFYKTRNLNSNRVSQNKPEITMSEITLGRYVFERLKQVDVTTVFGLPGDFNLRLLDEIYEVEGMRWAGNCNELNASYAADAYARIKGMSCLITTFGVGELSALNGIAGSYAEHVGVLHIVGVPSVSAQAKQLLLHHTLGNGDFTVFHRMSANISETTAMITDLATAPSEIDRCIRTTYIRQRPVYLGLPSNFVDQMVPASLLDTPIDLALKPNDQQAEEEVISTLLEMIKDAKNPVILADACASRHDVKAETKKLIDITQFPSFVTPMGKGSIDEKHPRFGGVYVGTLSSPAVKEAVESADLVLSVGALLSDFNTGSFSYSYKTKNVVEFHSDHIKIRNATFPGVQMKFVLQSLLNKVGAVVKDYKPVPVPELPAPNAAVDPSTPLKQQWLWNQVGQFLQEGDIVLTETGTSAFGINQTHFPNNTYGISQVLWGSIGYATGACLGAVMAAEELDKSKRVILFTGEGSLQLTAQEISTMVRWNLKPYLFVLNNKGYTIEKLIHGPTAEYNEIQNWNHLDLLPTFGAKDYEAIRVSTTGEWNKLAEDKDFNKNSKIRLIELMLPVMDAPESLVKQAQLTAATNAKSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCD7</accession>
    <name>C5DCD7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0B02222p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0B02222g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551886.1">
        <property type="nucleotide sequence ID" value="XM_002551840.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCD7"/>
    <dbReference type="FunCoup" id="C5DCD7">
        <property type="interactions" value="40"/>
    </dbReference>
    <dbReference type="GeneID" id="8290716"/>
    <dbReference type="KEGG" id="lth:KLTH0B02222g"/>
    <dbReference type="eggNOG" id="ENOG502QTIE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017094_2_0_1"/>
    <dbReference type="InParanoid" id="C5DCD7"/>
    <dbReference type="OMA" id="LIKPIPH"/>
    <dbReference type="OrthoDB" id="5563754at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058155">
        <property type="entry name" value="Skg3/CAF120-like_PH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25381">
        <property type="entry name" value="PH_26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Skg3/CAF120-like PH-like" evidence="2">
        <location>
            <begin position="392"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="314"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="315"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="340"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="350"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR21448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="530" mass="59056" checksum="805CA4196138A273" modified="2009-07-28" version="1">MKSFLGLTKLGNSGRKKVAPDQFSHIKSGMETMGTQDYEPLKPLLILLDCQKRKPYVSWDEGNELLLQVRGLTEVLPVRSLALQGTQLRVIVEPEDNGYDNVMVLDVANGVSPSGWKLGQTSLELNDGAMVFLCRSVEAQAHLQFLYDMCMLSRFESMSLYKALTATVISTHGSRISDISSILMSQRSYKDWCYISINGEWVKAWCHVDRSPKHSDSRKGRHQVKFFRDDKSLTAKNLICYIPDCGDIEDLFFVEDQSADLTQGSPDDGFGDLHFQNHLNSVRDQKVPLQMSLEPLLTRLTTLRFIGDVYWPAPSSNSTSSRSRSSTVLSFGAGNSNNESPKKSRDRRAMSTSSTSMFTSPKKSVKHTRTRSTASVTSTVHHADVNDACAITSELLIKPIPHSGVTHLESLIRAALPMMGCLSLYGRPVHFKNTREDIDSLLFALPKLPVIDYFAAEELPLLFQYVGSVNSTAGSLNKTLTAYKRFLHERLQEPGRDKRSFTTLSDLLQNNSKNTYGVGSSRSTSSSPLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCH6</accession>
    <name>C5DCH6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0B03146g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 10">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the oligosaccharyltransferase (OST) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Endoplasmic reticulum membrane</location>
            <topology evidence="2 10">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the OST1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551925.1">
        <property type="nucleotide sequence ID" value="XM_002551879.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCH6"/>
    <dbReference type="FunCoup" id="C5DCH6">
        <property type="interactions" value="1162"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCH6"/>
    <dbReference type="GeneID" id="8290758"/>
    <dbReference type="KEGG" id="lth:KLTH0B03146g"/>
    <dbReference type="eggNOG" id="KOG2291">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031381_1_0_1"/>
    <dbReference type="InParanoid" id="C5DCH6"/>
    <dbReference type="OMA" id="RYEYARE"/>
    <dbReference type="OrthoDB" id="310030at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008250">
        <property type="term" value="C:oligosaccharyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018279">
        <property type="term" value="P:protein N-linked glycosylation via asparagine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007676">
        <property type="entry name" value="Ribophorin_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21049:SF0">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21049">
        <property type="entry name" value="RIBOPHORIN I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04597">
        <property type="entry name" value="Ribophorin_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="459"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR21487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="485" mass="55038" checksum="343916F4CB5B034B" modified="2009-07-28" version="1">MYYKCSSLLKTVGEMLSATRLAAIFLLFLNFVVGDLDAGIIPKLWENLELHRTIDVSRSYTQELVDIRIKNIHDEPVRQYYFVLPEEVFDAISLFSSTLKGVDAYIESAIMPEQNFVANGRTIKVGVIGLPSPIEPGHETSILLNLVYNSRYQPYPGHVDLGEEQKLLLRTNRFPISAYNTQKYTLEFQGSSSFEELQSFNGEEHAGMVANGKMVVGPFDSIMPYEDFSPIEVVFEHNTPLPRVSHLKRDIWVSHWASSLQFEEYYELINDAASLKSGFSRADYMKGQHALKKGGHLTALEMILPDSSEAHYCTDLVGAVSTFKVLKDHFFLKPRYPLFGNWKYNFTVGWTNQLSQFLHSQEDGSDTYILSFPILNGPADTVYDSVSLSIYLPEGAEVLDVWCPLPVQKTEVTTEKSYFDLNQGHTKVNIELKNLIDRIAGSEAFVRYKVTSTTFYKKPFSIAVSVFTALMAYFFLKHISLMIDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCJ6</accession>
    <name>C5DCJ6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transcription initiation factor IIF subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0B03586g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the TFIIF alpha subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551945.1">
        <property type="nucleotide sequence ID" value="XM_002551899.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCJ6"/>
    <dbReference type="FunCoup" id="C5DCJ6">
        <property type="interactions" value="193"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCJ6"/>
    <dbReference type="GeneID" id="8290778"/>
    <dbReference type="KEGG" id="lth:KLTH0B03586g"/>
    <dbReference type="eggNOG" id="KOG2393">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020322_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCJ6"/>
    <dbReference type="OMA" id="FEEFYPW"/>
    <dbReference type="OrthoDB" id="76676at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005674">
        <property type="term" value="C:transcription factor TFIIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001096">
        <property type="term" value="F:TFIIF-class transcription factor complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008851">
        <property type="entry name" value="TFIIF-alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011039">
        <property type="entry name" value="TFIIF_interaction"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13011:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIF SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13011">
        <property type="entry name" value="TFIIF-ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05793">
        <property type="entry name" value="TFIIF_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50916">
        <property type="entry name" value="Rap30/74 interaction domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="26"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="142"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="240"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="388"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="481"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="560"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="39"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="53"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="151"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="193"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="240"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="396"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="428"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="451"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="507"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="521"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR21507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="659" mass="74836" checksum="15F422313B1D745A" modified="2009-07-28" version="1">MPNPLKSSNSASPFIKREQLRRDFLRSRLKKNGTPRPYVKKEDPEAYDERTRQLLSGGQSHSGIKREEGSQYNEFPLRAVSHEQLENIRTHILKFQSKKKVDPQRDMHLPVRLHRKDTRNLQFQLTRAEIVQRQKEIAEYKMKADSERNGGEQSQAGSSATNLQSDSNVASSAAQTPVETPGDTPADTPVKQESVKEEHGSSSMAIKKEGADLPATKLEEAGVAEDPTKVGMVKYDGKEKPLETESKEDPFADVAPDGGGRFRRGNTKRKTRQLKLLDENAKRLRFEEFYPWVLEDFDGLNTWVGSYEAGNSDSYVLLSVENDGSFTMIPADKVYKFTARNKYATLTIDEAEKRMEKNGKGIPRWLMKHLDDIGTTTTRYDRTQRKLRAVDARQGEEDDRNDNSEVELDFDEEFADDEEAPIIDGDEKENKESEQRIKKEMLQANAMGLRDDDEEADDDLFGEKKIDDEGERVMKALQKSDMAALYESDDESKSNPYLSESEGENTEEAKAKDENQENGKSSRKGSPKKKSSRGSPSGPRIYVKSIKDFQLVLRADKSVLRNFPEGNWNPHAKDEKSRTPGAASSPSSPPIKIEDDIENPDLLTERDIIDVVTGRQMTLKQLIKELKEKVAKHPSNKDRMKYLVKRLVRLNNGYLELNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCX2</accession>
    <name>C5DCX2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0B06490p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0B06490g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552071.1">
        <property type="nucleotide sequence ID" value="XM_002552025.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCX2"/>
    <dbReference type="FunCoup" id="C5DCX2">
        <property type="interactions" value="32"/>
    </dbReference>
    <dbReference type="GeneID" id="8290910"/>
    <dbReference type="KEGG" id="lth:KLTH0B06490g"/>
    <dbReference type="eggNOG" id="ENOG502S9PK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_192397_1_0_1"/>
    <dbReference type="InParanoid" id="C5DCX2"/>
    <dbReference type="OMA" id="CLIQQNY"/>
    <dbReference type="OrthoDB" id="4039633at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR21633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="69" mass="8056" checksum="49BE5E017131CE4D" modified="2009-07-28" version="1">MSEKSCPQCGEELKKCLIQQNYSLIICPNEGCCYPFNEREAIDNIVYTKDAEILNAAKRRLEEEEQQKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDN0</accession>
    <name>C5DDN0_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 4">Proteasome assembly chaperone 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0C02332g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in 20S proteasome assembly.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the 20S proteasome chaperone.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the PSMG2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552329.1">
        <property type="nucleotide sequence ID" value="XM_002552283.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDN0"/>
    <dbReference type="FunCoup" id="C5DDN0">
        <property type="interactions" value="127"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDN0"/>
    <dbReference type="GeneID" id="8291189"/>
    <dbReference type="KEGG" id="lth:KLTH0C02332g"/>
    <dbReference type="eggNOG" id="KOG3112">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062640_2_1_1"/>
    <dbReference type="InParanoid" id="C5DDN0"/>
    <dbReference type="OMA" id="PISWKGV"/>
    <dbReference type="OrthoDB" id="10260712at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10900">
        <property type="entry name" value="PAC-like subunit"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019151">
        <property type="entry name" value="Proteasome_assmbl_chaperone_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016562">
        <property type="entry name" value="Proteasome_assmbl_chp_2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038389">
        <property type="entry name" value="PSMG2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12970">
        <property type="entry name" value="PROTEASOME ASSEMBLY CHAPERONE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12970:SF1">
        <property type="entry name" value="PROTEASOME ASSEMBLY CHAPERONE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09754">
        <property type="entry name" value="PAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF010044">
        <property type="entry name" value="UCP010044"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00019186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR010044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR21891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="272" mass="30567" checksum="B75CE28D5335E830" modified="2009-07-28" version="1">MNTLLLPLVSTGNVPQLMTDLVLHSLDSEFEFVRELDSLYVYPFTGPIDYVEDSDSSLYRTSPEKTFTTPVELFFNPKLKLYIIQQRSPILQPYENQFCKNVIAPLIEELNIGSLVVTDATDNFGEHIAAASTRRSPYSFGVCQVSQIDDLSGEFQQKLQLHDTGATPVNNTLFQFSSQSFQSGISTEQFIFKLCYHLLHTQKVSEILKEIRYFNIYVQEGDNSEDAAAACTQVPQVITGFPSITTLRAPVSWKGVYGTRDAPGTFEEGLYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDZ5</accession>
    <name>C5DDZ5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">DNA repair and recombination protein RDH54</fullName>
            <ecNumber evidence="3">3.6.4.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">RAD homolog 54</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Recombination factor TID1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Two hybrid interaction with DMC1 protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="ordered locus">KLTH0C05038g</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Involved in the recombinational repair of double-strand breaks (DSB) in DNA during mitosis and meiosis. Has DNA dependent ATPase activity. Promotes D-loop (displacement loop) formation with RAD51 recombinase. Modifies the topology of double-stranded DNA during the D-loop reaction to facilitate the invasion of the homologous duplex molecule by the initiating single-stranded DNA substrate. Required for adaptation from G2/M checkpoint arrest induced by a double strand break, by participating in monitoring the extent of single-stranded DNA produced by resection of DNA ends. This role is distinct from its roles in recombination. Promotes colocalization of RAD51 and DMC1 during meiotic recombination. Involved in crossover interference.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="18">Interacts with RAD51 and DMC1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="3"/>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552444.1">
        <property type="nucleotide sequence ID" value="XM_002552398.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDZ5"/>
    <dbReference type="FunCoup" id="C5DDZ5">
        <property type="interactions" value="68"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDZ5"/>
    <dbReference type="GeneID" id="8291311"/>
    <dbReference type="KEGG" id="lth:KLTH0C05038g"/>
    <dbReference type="eggNOG" id="KOG0390">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000315_10_1_1"/>
    <dbReference type="InParanoid" id="C5DDZ5"/>
    <dbReference type="OMA" id="FTIMYRK"/>
    <dbReference type="OrthoDB" id="413460at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015616">
        <property type="term" value="F:DNA translocase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18004">
        <property type="entry name" value="DEXHc_RAD54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000058">
        <property type="entry name" value="RDH54p DNA-dependent ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.850">
        <property type="entry name" value="SWI2/SNF2 ATPases, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050496">
        <property type="entry name" value="SNF2_RAD54_helicase_repair"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45629:SF7">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45629">
        <property type="entry name" value="SNF2/RAD54 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="13" id="KW-0234">DNA repair</keyword>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0347">Helicase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="15" id="KW-0469">Meiosis</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="24">
        <location>
            <begin position="282"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="25">
        <location>
            <begin position="616"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="20"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="20"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00060154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00065350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00072543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00076096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00077864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00082011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EMBL" id="CAR22006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="894" mass="99817" checksum="C4C44999157B17C0" modified="2009-07-28" version="1">MRVPQYVNKPFKSPVICKKSVSTTESPTPTQQMKAPAVVRPLSKRAPSDVAPSINKKAAQGVAYYSIMYRKRSTKKHKTWDGDGTAIEMSNGSFRFYKDDGRFFGSGSLGKGEDKFERIFTCSGCEVQLDHQIEDEQEVSKLEEILGSSTKSTNQIVKPSLGHKIKQGTSSRSPSPGIQVVHYLPTQKFKPVYRNSQSDSFESTPGAANARRFPPIFDPSQIEDPLIMNTFTGAECDVIVDPILSKHLRPHQRVGVKFMYDCVMGLSRPSQHEDDGKTSCLLQKTDEVQGCLLADEMGLGKTLMTIALIWTLLKQTPTPSKETTSQFGIPLQGLCNKVLVVCPVTLIANWKREFSKWLGLNRIGILTLDSKSTPEKDKLHVRNFLRVQRTYQVLILGYEKLLSVTEALQQESSSIGLMVCDEGHRLKNGQSKTLSALNSLDISRKVLLTGTPIQNDLTEFFTIIDFLNCGIMGNFSQFKRDYINPITRARDVNNKYNETIQTRGKDRTDELIDATKKFTLRRTADTISKFLPPKTDVVLFCKPTKSQIQAFEKILTNAQLDLSSITPSSSLALITLFKKICNSPSLISQDSYYLENIKSKSDLTGCLGLDSGKLRVLLALLENIRSSYPQEKVVIVSNYTQTLDIIQNILTSNRFAFTRLDGNTPTKERDRIVRAFNTVPSLFAFLLSAKSGGVGLNLIGASRLVLFDNDWNPAIDLQAMSRIHRDGQRRPCFIYRLLTTGCIDEKIFQRQLMKISLSFRFMGDQESSSSIKADDNLFETDDLKDLFTVLTSTKSNTHDLICACHGQKEHVSDSKVTAATMAETGAKGWTSAMDLMASMEKAESEKIAEKAALVQQCLVDYEHIETLKAHEIISEAALESQTDVTFAFIKSSKI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-03-08" modified="2025-06-18" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>C5DE77</accession>
    <name>AIM11_LACTC</name>
    <protein>
        <recommendedName>
            <fullName>Altered inheritance of mitochondria protein 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">AIM11</name>
        <name type="ordered locus">KLTH0C06930g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AIM11 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552526.1">
        <property type="nucleotide sequence ID" value="XM_002552480.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DE77"/>
    <dbReference type="SMR" id="C5DE77"/>
    <dbReference type="FunCoup" id="C5DE77">
        <property type="interactions" value="34"/>
    </dbReference>
    <dbReference type="GeneID" id="8291395"/>
    <dbReference type="KEGG" id="lth:KLTH0C06930g"/>
    <dbReference type="eggNOG" id="ENOG502SAK0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118700_0_0_1"/>
    <dbReference type="InParanoid" id="C5DE77"/>
    <dbReference type="OMA" id="RFAYKST"/>
    <dbReference type="OrthoDB" id="4088121at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038814">
        <property type="entry name" value="AIM11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39136">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39136:SF1">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000405649" description="Altered inheritance of mitochondria protein 11">
        <location>
            <begin position="1"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="150" mass="16925" checksum="91190A890C6B819B" modified="2009-07-28" version="1">MASVQLSSRDISVFSNEYKERRRLQMMRFFGATAFTLISARLAFRGVQSRKYVPNMFQLNHKPPTYSFQGEAVSALAFGTGLATGTFSMLVFGTCWVWDISSLAEFTLKMKKLMGEPVTDQALLENTPMDEDTRKVAEALEDMLKGSRKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2023-06-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEB1</accession>
    <name>C5DEB1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0C07744p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0C07744g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552560.1">
        <property type="nucleotide sequence ID" value="XM_002552514.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEB1"/>
    <dbReference type="GeneID" id="8291432"/>
    <dbReference type="KEGG" id="lth:KLTH0C07744g"/>
    <dbReference type="HOGENOM" id="CLU_1441287_0_0_1"/>
    <dbReference type="InParanoid" id="C5DEB1"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="188" mass="21004" checksum="3B1667D63E1E1205" modified="2009-07-28" version="1">MPPGFARVPTRHERNPNEIADPDQFWPNARAAGPSKVRLAILPVCRRRAAWSELQRANFTEKPPRILIRGPFGDESQLVTDFSFVPLNNSSFPLQVEAQLNFPGGNEVVQSPASGQTDLRKARTSPLELPPARPLLCHKIGSSSSFAQYDLRPNGYAVFEPEDESVKNYLNYTYLLLQVYSSDDIACC</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEC9</accession>
    <name>C5DEC9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">ATP synthase subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0C08162g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and the central stalk which is part of the complex rotary element. The gamma subunit protrudes into the catalytic domain formed of alpha(3)beta(3). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) and CF(0) have multiple subunits.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the ATPase gamma chain family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552578.1">
        <property type="nucleotide sequence ID" value="XM_002552532.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEC9"/>
    <dbReference type="FunCoup" id="C5DEC9">
        <property type="interactions" value="1026"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEC9"/>
    <dbReference type="GeneID" id="8291451"/>
    <dbReference type="KEGG" id="lth:KLTH0C08162g"/>
    <dbReference type="eggNOG" id="KOG1531">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050669_4_1_1"/>
    <dbReference type="InParanoid" id="C5DEC9"/>
    <dbReference type="OMA" id="MQITSAM"/>
    <dbReference type="OrthoDB" id="239812at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12151">
        <property type="entry name" value="F1-ATPase_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.80:FF:000001">
        <property type="entry name" value="ATP synthase gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1380.10:FF:000003">
        <property type="entry name" value="ATP synthase subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1380.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.80">
        <property type="entry name" value="ATP synthase, gamma subunit, helix hairpin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035968">
        <property type="entry name" value="ATP_synth_F1_ATPase_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000131">
        <property type="entry name" value="ATP_synth_F1_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023632">
        <property type="entry name" value="ATP_synth_F1_gsu_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01146">
        <property type="entry name" value="ATPsyn_F1gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11693">
        <property type="entry name" value="ATP SYNTHASE GAMMA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11693:SF22">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT GAMMA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00231">
        <property type="entry name" value="ATP-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039089">
        <property type="entry name" value="ATP_synthase_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00126">
        <property type="entry name" value="ATPASEGAMMA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52943">
        <property type="entry name" value="ATP synthase (F1-ATPase), gamma subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00153">
        <property type="entry name" value="ATPASE_GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 13" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="11 13" id="KW-0139">CF(1)</keyword>
    <keyword evidence="6 13" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="8 13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR22140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="293" mass="31951" checksum="8A8C9F2684F1E698" modified="2009-07-28" version="1">MFVRSGANCARSVAGPARTFATLREIEMRLKSIKNIEKITKTMKIVASTRLSKAERAKVTAREYSSAERQFYDSAETKPLEGEGEKKDLVIAVTSDKGLCGSIHSQLSKAVRNYLAKTPGADVVAVGDKVKAQLLRTHSDRVKLAFNGIGKEAPTFEESSLIANKILQLGGGNYSKISIFYNDPVSSLSFEPRVKPVFNETAIEQSPSFAKFEIDADSKVSQDMFEFTLANQILAAMADGYAAEISARRNAMDNASKNAGDMINKYSILYNRTRQAVITNELVDIITGASSLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEH4</accession>
    <name>C5DEH4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0C09262p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0C09262g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552623.1">
        <property type="nucleotide sequence ID" value="XM_002552577.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEH4"/>
    <dbReference type="FunCoup" id="C5DEH4">
        <property type="interactions" value="125"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEH4"/>
    <dbReference type="GeneID" id="8291501"/>
    <dbReference type="KEGG" id="lth:KLTH0C09262g"/>
    <dbReference type="eggNOG" id="ENOG502RZX7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047273_0_0_1"/>
    <dbReference type="InParanoid" id="C5DEH4"/>
    <dbReference type="OMA" id="XHIQSKE"/>
    <dbReference type="OrthoDB" id="20109at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005655">
        <property type="term" value="C:nucleolar ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000171">
        <property type="term" value="F:ribonuclease MRP activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034965">
        <property type="term" value="P:intronic box C/D snoRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013241">
        <property type="entry name" value="RNase_P_Pop3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28272">
        <property type="entry name" value="RIBONUCLEASES P/MRP PROTEIN SUBUNIT POP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28272:SF1">
        <property type="entry name" value="RIBONUCLEASES P_MRP PROTEIN SUBUNIT POP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08228">
        <property type="entry name" value="RNase_P_pop3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR22185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="196" mass="21523" checksum="C924D0042A69EB93" modified="2009-07-28" version="1">MSSLKEIQQNTARRKQVYKPILDNPFTDEAHMWPYVRDQPLVAELVRSHVIEPLAHAHAMKLQGTLNAHFGFNSVVQYLEAPPTSDSVFLFVCNRDSVPSVLLSQIPILAQVSSCDITLVPLPRGTSDSLGACTSTDHDGLLLVVQDGKFDPHIATQLSAHVDHVKEKPWLLFSKSRIAALSSTAAISSKSKSKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>C5DER6</accession>
    <name>C5DER6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 13">Phosphoacetylglucosamine mutase</fullName>
            <shortName evidence="13">PAGM</shortName>
            <ecNumber evidence="4 13">5.4.2.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Acetylglucosamine phosphomutase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">N-acetylglucosamine-phosphate mutase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ordered locus">KLTH0C11506g</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the conversion of GlcNAc-6-P into GlcNAc-1-P during the synthesis of uridine diphosphate/UDP-GlcNAc, a sugar nucleotide critical to multiple glycosylation pathways including protein N- and O-glycosylation.</text>
    </comment>
    <comment type="function">
        <text evidence="13">Catalyzes the conversion of GlcNAc-6-P into GlcNAc-1-P during the synthesis of uridine diphosphate/UDP-GlcNAc, which is a biosynthetic precursor of chitin and also supplies the amino sugars for N-linked oligosaccharides of glycoproteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>N-acetyl-alpha-D-glucosamine 1-phosphate = N-acetyl-D-glucosamine 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:23804"/>
            <dbReference type="ChEBI" id="CHEBI:57513"/>
            <dbReference type="ChEBI" id="CHEBI:57776"/>
            <dbReference type="EC" id="5.4.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13 16">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="13 16">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route I): step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.3" evidence="4 13"/>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552715.1">
        <property type="nucleotide sequence ID" value="XM_002552669.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DER6"/>
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        <property type="interactions" value="659"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DER6"/>
    <dbReference type="GeneID" id="8291602"/>
    <dbReference type="KEGG" id="lth:KLTH0C11506g"/>
    <dbReference type="eggNOG" id="KOG2537">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022890_1_0_1"/>
    <dbReference type="InParanoid" id="C5DER6"/>
    <dbReference type="OMA" id="WEAYATK"/>
    <dbReference type="OrthoDB" id="1928at2759"/>
    <dbReference type="UniPathway" id="UPA00113">
        <property type="reaction ID" value="UER00530"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004610">
        <property type="term" value="F:phosphoacetylglucosamine mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006048">
        <property type="term" value="P:UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03086">
        <property type="entry name" value="PGM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.50:FF:000003">
        <property type="entry name" value="Phosphoacetylglucosamine mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphoacetylglucosamine mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphoacetylglucosamine mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.120.10">
        <property type="entry name" value="Alpha-D-Glucose-1,6-Bisphosphate, subunit A, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.50">
        <property type="entry name" value="Alpha-D-phosphohexomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005843">
        <property type="entry name" value="A-D-PHexomutase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016066">
        <property type="entry name" value="A-D-PHexomutase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049023">
        <property type="entry name" value="AMG1_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049022">
        <property type="entry name" value="AMG1_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016657">
        <property type="entry name" value="PAGM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45955">
        <property type="entry name" value="PHOSPHOACETYLGLUCOSAMINE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45955:SF1">
        <property type="entry name" value="PHOSPHOACETYLGLUCOSAMINE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21405">
        <property type="entry name" value="AMG1_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21404">
        <property type="entry name" value="AMG1_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00408">
        <property type="entry name" value="PGM_PMM_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016408">
        <property type="entry name" value="PAGM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="Phosphoglucomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="Phosphoglucomutase, first 3 domains"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00710">
        <property type="entry name" value="PGM_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="9 13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7 13 16" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 13 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="18">
        <location>
            <begin position="57"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoacetylglucosamine mutase AMG1" evidence="20">
        <location>
            <begin position="188"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoacetylglucosamine mutase AMG1" evidence="19">
        <location>
            <begin position="306"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase C-terminal" evidence="17">
        <location>
            <begin position="464"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphoserine intermediate" evidence="14">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <feature type="binding site" description="via phosphate group" evidence="16">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="287"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="381"/>
            <end position="383"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="509"/>
            <end position="513"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="518"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00070218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR016408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR016408-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR016408-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR016408-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF21404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF21405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="CAR22277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="544" mass="60276" checksum="32B7C5A09FACD1CA" modified="2009-07-28" version="1">MLDTKTLETLYETHCKSGFTYAYGTAGFRYKADVLDTVMFTTGVLAGLRSIYLEGRTVGVMITASHNPPEDNGVKIVDPKGEMLEQSWESYATDLANSATQSFTAFLAYLQNLVSELGLDTDALGSLAVARDSRESGPRLLHALLNGSRVLPNIRITDYGLLTTPQLHFLTSKRNELPNDTAIDRFFYYKEFLSAWDDITALCGIQSLPYKLEIDAANGIGAPRVKEMLSTHKFFDGVFRVVNDDWETPASLNHLCGADYVKTNQRLPYGIQANNDCQRICCSFDGDADRIVFYFCDSEGQFRLLDGDKMATLFAKFFQDILSSANLQERLSLGVVQTAYANGSSTHYIERVLGIPVTCTPTGVKHLHHEAINYDIGVYFEANGHGTVVFSKKFDEVVNSGLSNKHQSDSSLQSLEVLSKFANLINQTVGDAISDMMGVLAVLSILKWTPQKWNQEYTDLPNKLVKVLVPDRTVFKTTNAERQLLSPLGLQPKINALVAEYHQARSFVRASGTEDAVRVYAEASTREEADSLASRVSQVVEHNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>C5DF65</accession>
    <name>C5DF65_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GMP synthase [glutamine-hydrolyzing]</fullName>
            <ecNumber evidence="5">6.3.5.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">GMP synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Glutamine amidotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ordered locus">KLTH0D12584g</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Catalyzes the conversion of xanthine monophosphate (XMP) to GMP in the presence of glutamine and ATP through an adenyl-XMP intermediate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>XMP + L-glutamine + ATP + H2O = GMP + L-glutamate + AMP + diphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:11680"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="6.3.5.2" evidence="5"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553258.1">
        <property type="nucleotide sequence ID" value="XM_002553212.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DF65"/>
    <dbReference type="FunCoup" id="C5DF65">
        <property type="interactions" value="1131"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DF65"/>
    <dbReference type="MEROPS" id="C26.957"/>
    <dbReference type="GeneID" id="8295498"/>
    <dbReference type="KEGG" id="lth:KLTH0D12584g"/>
    <dbReference type="eggNOG" id="KOG1622">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014340_0_5_1"/>
    <dbReference type="InParanoid" id="C5DF65"/>
    <dbReference type="OMA" id="IWQSFAV"/>
    <dbReference type="OrthoDB" id="1724632at2759"/>
    <dbReference type="UniPathway" id="UPA00189">
        <property type="reaction ID" value="UER00296"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003921">
        <property type="term" value="F:GMP synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01742">
        <property type="entry name" value="GATase1_GMP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01997">
        <property type="entry name" value="GMP_synthase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.10:FF:000002">
        <property type="entry name" value="GMP synthase [glutamine-hydrolyzing]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000001">
        <property type="entry name" value="GMP synthase [glutamine-hydrolyzing]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000001">
        <property type="entry name" value="GMP synthase [glutamine-hydrolyzing]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00344">
        <property type="entry name" value="GMP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001674">
        <property type="entry name" value="GMP_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004739">
        <property type="entry name" value="GMP_synth_GATase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022955">
        <property type="entry name" value="GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025777">
        <property type="entry name" value="GMPS_ATP_PPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022310">
        <property type="entry name" value="NAD/GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00884">
        <property type="entry name" value="guaA_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00888">
        <property type="entry name" value="guaA_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000848">
        <property type="entry name" value="PRK00074.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11922:SF2">
        <property type="entry name" value="GMP SYNTHASE [GLUTAMINE-HYDROLYZING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11922">
        <property type="entry name" value="GMP SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00958">
        <property type="entry name" value="GMP_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02540">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00097">
        <property type="entry name" value="ANTSNTHASEII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00096">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54810">
        <property type="entry name" value="GMP synthetase C-terminal dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51553">
        <property type="entry name" value="GMPS_ATP_PPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="9 18" id="KW-0332">GMP biosynthesis</keyword>
    <keyword evidence="7" id="KW-0436">Ligase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10 18" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GMPS ATP-PPase" evidence="19">
        <location>
            <begin position="203"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="231"/>
            <end position="237"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00021562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00031356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00044933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CAR22820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="525" mass="58700" checksum="2102846504634C18" modified="2009-07-28" version="1">MSDVEQVSSVFDTILVLDFGSQYSHLITRRLREFNVYAEMLPCTQKISELGWKPKGVILSGGPYSVYAEDAPHVDMDVFKLDVPILGICYGMQELAWINGKQVARGEKREYGPATLKVEDASSPLFEGMDHSRVWMSHGDKLHGLPTGYKVIATSDNSPFCGITHEEKPIFGIQFHPEVTHSTQGKTLLKNFAVNICKANQNWTMENFIDTEIKRIRELVGPTAEVIGAVSGGVDSTVAAKLMTEAIGERFHAILVDNGVLRLNEAANVKKTLGEGLKINLTVVDAADEFLDKLKGVTDPEKKRKIIGNTFIHVFEREAAKIQPKSGEEIEFLLQGTLYPDVIESISFKGPSQTIKTHHNVGGLLEDMKLKLIEPLRELFKDEVRHLGELLGISHELVWRHPFPGPGIAIRVLGEVTREQVEIARKADFIYIEEIRKAGLYNKISQAFACLLPVKSVGVMGDQRTYEQVIALRAIETTDFMTADWFPFEHDFLKRVASRIVNEVDGVARVTYDITSKPPATVEWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFE9</accession>
    <name>C5DFE9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0D14542p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0D14542g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553342.1">
        <property type="nucleotide sequence ID" value="XM_002553296.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFE9"/>
    <dbReference type="FunCoup" id="C5DFE9">
        <property type="interactions" value="45"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFE9"/>
    <dbReference type="GeneID" id="8295586"/>
    <dbReference type="KEGG" id="lth:KLTH0D14542g"/>
    <dbReference type="eggNOG" id="ENOG502S03S">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101036_1_1_1"/>
    <dbReference type="InParanoid" id="C5DFE9"/>
    <dbReference type="OMA" id="TMQEDHE"/>
    <dbReference type="OrthoDB" id="2209940at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072380">
        <property type="term" value="C:TRC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006620">
        <property type="term" value="P:post-translational protein targeting to endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.150">
        <property type="entry name" value="Haem-degrading domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005624">
        <property type="entry name" value="PduO/GlcC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038084">
        <property type="entry name" value="PduO/GlcC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010371">
        <property type="entry name" value="YBR137W-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28255">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28255:SF1">
        <property type="entry name" value="UPF0303 PROTEIN YBR137W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03928">
        <property type="entry name" value="HbpS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF008757">
        <property type="entry name" value="UCP008757"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143744">
        <property type="entry name" value="GlcG-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR22904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="184" mass="20537" checksum="1713F647E55B11A6" modified="2009-07-28" version="1">MLEFDKKFCDKMPSLPLEELRALEREYVFARFDFEVAFDLGCKVRQWARELFPADALVVDLSLPSGQCVFHATTNAGTALDNDIWVARKRRTAVRFGCSSFVMGRKLAAKQQQRASLTMQDAYFVDPAEYAFHGGALPLRVEGLDAPVAVLTVSGLAQATDHAFALACAREFAQRLAQRELDLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFG4</accession>
    <name>C5DFG4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0D14894p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0D14894g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553357.1">
        <property type="nucleotide sequence ID" value="XM_002553311.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFG4"/>
    <dbReference type="FunCoup" id="C5DFG4">
        <property type="interactions" value="32"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFG4"/>
    <dbReference type="GeneID" id="8295601"/>
    <dbReference type="KEGG" id="lth:KLTH0D14894g"/>
    <dbReference type="eggNOG" id="ENOG502S6I5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092540_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFG4"/>
    <dbReference type="OMA" id="WTETRKW"/>
    <dbReference type="OrthoDB" id="5576875at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053274">
        <property type="entry name" value="Fluconazole_resistance"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025124">
        <property type="entry name" value="Gag1-like_clamp"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28065:SF1">
        <property type="entry name" value="DUF4050 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28065">
        <property type="entry name" value="FREQUENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13259">
        <property type="entry name" value="clamp_Gag1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gag1-like clamp" evidence="2">
        <location>
            <begin position="131"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR22919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="214" mass="24022" checksum="E5D40AFEA81A3B84" modified="2009-07-28" version="1">MVPKARKSSSSPLGGSIRQFLHRCKVGLHKMSKEALDTESSEEYNTVNGLFDTGPSKGASQERTSEERNSSTSVTLTREMPLTDEEDELDSEVEEPGDAPGAKDAQTFATFDVVQECAKLRAATGEPFVRAEEVWRRRRDLWVSTTNGSYLETAEKNREVFKEIAPRQYVRIYRKLVVDGVPLKRPLNLQDAMQVVNAGWVETQKWERAAKGLA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-06-28" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFG5</accession>
    <name>BL1S1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName>Biogenesis of lysosome-related organelles complex 1 subunit BLS1</fullName>
            <shortName>BLOC-1 subunit BLS1</shortName>
        </recommendedName>
        <alternativeName>
            <fullName>BLOS1-homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">BLS1</name>
        <name type="ordered locus">KLTH0D14916g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the biogenesis of lysosome-related organelles complex-1 (BLOC-1), a complex involved in endosomal cargo sorting.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the biogenesis of lysosome-related organelles complex-1 (BLOC-1).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BLOC1S1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553358.1">
        <property type="nucleotide sequence ID" value="XM_002553312.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFG5"/>
    <dbReference type="SMR" id="C5DFG5"/>
    <dbReference type="FunCoup" id="C5DFG5">
        <property type="interactions" value="24"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFG5"/>
    <dbReference type="GeneID" id="8295602"/>
    <dbReference type="KEGG" id="lth:KLTH0D14916g"/>
    <dbReference type="eggNOG" id="ENOG502S787">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_150164_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFG5"/>
    <dbReference type="OMA" id="IEANHAY"/>
    <dbReference type="OrthoDB" id="20018at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0967">Endosome</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000410631" description="Biogenesis of lysosome-related organelles complex 1 subunit BLS1">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="109" mass="12576" checksum="1BD0DD2C787DBD95" modified="2009-07-28" version="1">MSKASKEALLERALNKITKSQTNTNVAEAHEEIESNYAYINEKQLKRLVELHDTEFKDKCVVPLQRLYYKYSDTVLCDGDLQNWAELIDRDIRVLETTLAKVRDNQSGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFM5</accession>
    <name>C5DFM5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Endoplasmic reticulum-Golgi intermediate compartment protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0D16324g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Plays a role in transport between endoplasmic reticulum and Golgi.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Endoplasmic reticulum membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Endoplasmic reticulum-Golgi intermediate compartment membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Golgi apparatus membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ERGIC family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553418.1">
        <property type="nucleotide sequence ID" value="XM_002553372.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFM5"/>
    <dbReference type="FunCoup" id="C5DFM5">
        <property type="interactions" value="400"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFM5"/>
    <dbReference type="GeneID" id="8295665"/>
    <dbReference type="KEGG" id="lth:KLTH0D16324g"/>
    <dbReference type="eggNOG" id="KOG2667">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034705_2_1_1"/>
    <dbReference type="InParanoid" id="C5DFM5"/>
    <dbReference type="OMA" id="MTNHYLR"/>
    <dbReference type="OrthoDB" id="5541786at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033116">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045888">
        <property type="entry name" value="Erv"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012936">
        <property type="entry name" value="Erv_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039542">
        <property type="entry name" value="Erv_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10984">
        <property type="entry name" value="ENDOPLASMIC RETICULUM-GOLGI INTERMEDIATE COMPARTMENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10984:SF81">
        <property type="entry name" value="ER-DERIVED VESICLES PROTEIN ERV41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07970">
        <property type="entry name" value="COPIIcoated_ERV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13850">
        <property type="entry name" value="ERGIC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="29"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="287"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="Endoplasmic reticulum vesicle transporter N-terminal" evidence="7">
        <location>
            <begin position="4"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="Endoplasmic reticulum vesicle transporter C-terminal" evidence="6">
        <location>
            <begin position="147"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU369013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR22980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="340" mass="38901" checksum="33790C836E8D6DF5" modified="2009-07-28" version="1">MAGLRTFDAFPKTEEQHVRKSSKGGYTSILTYVFLIFIAWSEFGSFFGGYVDEQYGVSKDLREAVQINMDMFVHMPCQWLDVIVQDHTGDRKLVREELKMESIPFFLPFGTAVNERNEIASLGLDEVLAEAIPGQFRDQIDFGSEDESKEFNGCHVFGTITVNMVKGDLIIIPRSQSVRDFGRMPPDAINLSHVINEFSFGDFYPYIDNPLDRSARITAEHTTSFHYHTSVVPTIFQKLGAEVNTNQYSLSETKHETPPSGLRVPAIIFSYSFEALTITIRDERISFWQFIVRLVAILSFIVYIMTWAFTLLDMILVLLLGPKWSLRYQRDGRQKTGILE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFM6</accession>
    <name>C5DFM6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0D16346p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0D16346g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553419.1">
        <property type="nucleotide sequence ID" value="XM_002553373.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFM6"/>
    <dbReference type="FunCoup" id="C5DFM6">
        <property type="interactions" value="16"/>
    </dbReference>
    <dbReference type="GeneID" id="8295666"/>
    <dbReference type="KEGG" id="lth:KLTH0D16346g"/>
    <dbReference type="eggNOG" id="ENOG502S5T2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_122928_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFM6"/>
    <dbReference type="OMA" id="QYLWQEA"/>
    <dbReference type="OrthoDB" id="4067025at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="175" mass="19793" checksum="78C379DBC52BB8EE" modified="2009-07-28" version="1">MSNSRSGASSGSRGDGRSEICPQDKHDLKYLAANWQNNSQHTGTIRTEMMKQDVLNRMDKITGLMNADCKKASGAPEHEPVERLNWDELYEVSANVMDLYTKDVDECLSELEKFYRKQYLWQEAAFTIDSHRGAARIGAAEAWVIAKEAHLENKRQELSSSAQIIKNTLEGLSQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFP2</accession>
    <name>C5DFP2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FK506-binding protein</fullName>
            <ecNumber evidence="5">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0D16764g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FKBP-type PPIase family. FKBP3/4 subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="5"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553435.1">
        <property type="nucleotide sequence ID" value="XM_002553389.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFP2"/>
    <dbReference type="FunCoup" id="C5DFP2">
        <property type="interactions" value="611"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFP2"/>
    <dbReference type="GeneID" id="8295684"/>
    <dbReference type="KEGG" id="lth:KLTH0D16764g"/>
    <dbReference type="eggNOG" id="KOG0552">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022297_3_1_1"/>
    <dbReference type="InParanoid" id="C5DFP2"/>
    <dbReference type="OMA" id="CPPHMAY"/>
    <dbReference type="OrthoDB" id="77911at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.40:FF:000006">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.340">
        <property type="entry name" value="Nucleoplasmin core domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041232">
        <property type="entry name" value="NPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023566">
        <property type="entry name" value="PPIase_Fpr3/Fpr4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43811:SF19">
        <property type="entry name" value="39 KDA FK506-BINDING NUCLEAR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43811">
        <property type="entry name" value="FKBP-TYPE PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17800">
        <property type="entry name" value="NPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001473">
        <property type="entry name" value="FK506-bp_FPR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 6" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase FKBP-type" evidence="8">
        <location>
            <begin position="333"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="41"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="172"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="61"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="96"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="187"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="223"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="258"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR001473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR22997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="419" mass="47302" checksum="846066E777A25788" modified="2009-07-28" version="1">MSELLPLATYNLKVEPYTPTPAIDVTVPVTVRITLAAVDPEAVDKENKPSTLRIIKRNPDFDDDEDDILGGDFDEDELDNEESDEEEAQEEEKPAKKSKGKSKKQQKKEQEEEEEESEGSIEEEEAELDDEFQEYVVLTLSPKVQFQQTLDLVIAPEEEVQFVLTGSYPIHLTGNYVKHPFDQPQYGDDDSEEEDSEEYDSEEYDLTPDEDELLDDLEDVSDVESRIEELVQQDEQKNKNKNKNKKRKQDEKEDGDEEKAPEQPKETKKTKKEEPKKDKKEKKVEFKKNLEEGPSKKAAKEAAKETKPKTQALEGGIIVEDRVVGQGKTAKRGSKVGMRYIGKLKNGKVFDKNTSGKPFNFNLGRGEVIKGWDIGVAGMAVGGERRIVIPAPYAYGKQALPGIPANSELTFDVKLVSMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>C5DG54</accession>
    <name>C5DG54_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0D02508p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0D02508g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552834.1">
        <property type="nucleotide sequence ID" value="XM_002552788.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DG54"/>
    <dbReference type="FunCoup" id="C5DG54">
        <property type="interactions" value="45"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DG54"/>
    <dbReference type="GeneID" id="8295054"/>
    <dbReference type="KEGG" id="lth:KLTH0D02508g"/>
    <dbReference type="eggNOG" id="ENOG502S7IW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_765194_0_0_1"/>
    <dbReference type="InParanoid" id="C5DG54"/>
    <dbReference type="OMA" id="VSACDIM"/>
    <dbReference type="OrthoDB" id="4067660at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR22396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="354" mass="39460" checksum="13917DB70D28029A" modified="2009-07-28" version="1">MSSPQVSSTKLLEELLENKAFQPDSHKALESETENQLQDCLNAFLKGDCKGCLEKMFQAGLLRGEYLENDPKLWKLYLSACSQIKFDVLEVSLSKVGRSNFSKADVSRAQRILAQEPLCEQMAGVLMYLTCCYKCARADNSMEAMKNLEMASGDYIRKCSECSEHSGDTREANQVLELVWFYMFSLQGGRSHKRMSRILYEQLCKSAPAVPRVLQAPSKTNPAVTLENQLISRIEALAPEKPNIVQPLQQRKLKPTNSHPFSSDRTSEAIPVLAPVPTIIKPDKAASINTSKFKALITRLQPFLNSKSIPVLVFVLLLSLKKAQKVAGLPNKLAHILNVILRRLGGIINIILSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5DG77</accession>
    <name>C5DG77_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Conserved oligomeric Golgi complex subunit 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Component of oligomeric Golgi complex 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0D03014g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the COG4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552857.1">
        <property type="nucleotide sequence ID" value="XM_002552811.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DG77"/>
    <dbReference type="FunCoup" id="C5DG77">
        <property type="interactions" value="861"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DG77"/>
    <dbReference type="GeneID" id="8295077"/>
    <dbReference type="KEGG" id="lth:KLTH0D03014g"/>
    <dbReference type="eggNOG" id="KOG0412">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014853_3_0_1"/>
    <dbReference type="InParanoid" id="C5DG77"/>
    <dbReference type="OMA" id="RASECQQ"/>
    <dbReference type="OrthoDB" id="47059at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048682">
        <property type="entry name" value="COG4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048684">
        <property type="entry name" value="COG4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013167">
        <property type="entry name" value="COG4_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048680">
        <property type="entry name" value="COG4_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24016">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24016:SF0">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20662">
        <property type="entry name" value="COG4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08318">
        <property type="entry name" value="COG4_m"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20663">
        <property type="entry name" value="COG4_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00762">
        <property type="entry name" value="Cog4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="COG4 transport protein middle alpha-helical bundle" evidence="9">
        <location>
            <begin position="179"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR22419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="812" mass="92412" checksum="A3B19D1109F452BF" modified="2009-07-28" version="1">MDALDLDKLSTNLARYHTLLSKITTVSQLTKLQSAIENEQSSTARQLDAFLQPSHTNTNQAVRTLELNRARLTQTLSKLHHALESVAESKQLALQVSTRIRAVDRERQLVTRVLQFVSHVTSLRQNIFMVHQAIDRSDYKLAAAGIDTILKLPEESVYSEFARRVVPSAEIPEDPQDLLKQWINQLAQVFHDNFVKAAREQDITQLTAVFQLFPLIRKSDMGLDLYSKYVCDMIATQSRKVMTSTTSKSPGFFAQALLHLFKIVSTIINEHSKIIAKYYGKQHMVHIMAKVQREADMQAGLVLDSFFESRNFKEILQDIRAFKENVSPEEEGLTSNAPSMSEVSGIINESSSILQNWSMYCRFFALKWREFSDQSSEDPLTLPAPICDGIFYSKIHSSGFLSDLKKLNEFYLNRSFKKSLELEELPSLNEQISKAPLKHDDISSYGFSSIIEDLTVLIRSCLVVNVNTGESTLLGQFLDMLVRFLQNEYLIRFLQHRLRQLQPRLTSNVVLKKYVPQTESTMNSRAASPGAVEQSKLSHLRFNFKGAATSAFSNIQSNLQAVYADEETVLKLHQYLIYLNTLSIGCVFLQKLLTKELLQDNPQLLPDNFPFGDKSQSLVKKIEATEQTLLAQSSKLLQWGVRLLFDGVLQSKIQKILGPLFTQGTETEYLASTANFEDMSHLKQFSDGWLETMNPYINVLYKDCYAQLLTYAVDFLVAQISPRLWSLKLNELGSIKLDRELGALIAIVCGEHYLLREKFTKLTQIVLIAGFEDDDFDSETSDLKEEVIKGISWVLNPTERIRAGELRVDRRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DG89</accession>
    <name>C5DG89_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Glutamate--cysteine ligase</fullName>
            <ecNumber evidence="3 11">6.3.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 11">Gamma-ECS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9 11">Gamma-glutamylcysteine synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0D03322g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-cysteine + L-glutamate + ATP = gamma-L-glutamyl-L-cysteine + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13285"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58173"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the glutamate--cysteine ligase type 3 family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.2" evidence="3 11"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552869.1">
        <property type="nucleotide sequence ID" value="XM_002552823.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DG89"/>
    <dbReference type="FunCoup" id="C5DG89">
        <property type="interactions" value="423"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DG89"/>
    <dbReference type="GeneID" id="8295091"/>
    <dbReference type="KEGG" id="lth:KLTH0D03322g"/>
    <dbReference type="eggNOG" id="KOG3754">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010467_0_0_1"/>
    <dbReference type="InParanoid" id="C5DG89"/>
    <dbReference type="OMA" id="IAHMFIR"/>
    <dbReference type="OrthoDB" id="7939818at2759"/>
    <dbReference type="UniPathway" id="UPA00142">
        <property type="reaction ID" value="UER00209"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004357">
        <property type="term" value="F:glutamate-cysteine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006750">
        <property type="term" value="P:glutathione biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.50:FF:000006">
        <property type="entry name" value="Glutamate--cysteine ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.50:FF:000004">
        <property type="entry name" value="Glutamate-cysteine ligase Gcs1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.960">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.50">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.710">
        <property type="entry name" value="Glutamate cysteine ligase subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004308">
        <property type="entry name" value="GCS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11164">
        <property type="entry name" value="GLUTAMATE CYSTEINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11164:SF0">
        <property type="entry name" value="GLUTAMATE--CYSTEINE LIGASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03074">
        <property type="entry name" value="GCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 11" id="KW-0317">Glutathione biosynthesis</keyword>
    <keyword evidence="5 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="318"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR22431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="650" mass="75124" checksum="59507DCF761A44D0" modified="2009-07-28" version="1">MGLLSLGTPLEWPESRQYNDHVRKNGIEQLLYIFLAANGRKNDPLLWGDEIEYMMCKIDDAGRNAVLAVDDDDVLCALNTEDVGVCQAHNTSFHPEYGRYMIEATPARPYTGFMSNYVEENMGERRAIVDSKLEKRGVELLSLAVFPRMGCPGFTDLSESWDHHNSASRSLYLPDEIINRHARFPTLTANIRTRRGEKVCMNVPMYKDKRTPDHDDTVFVRDWFTIEDKEGPLASKPGHIYMDSMGFGMGCSCLQLTFQAPTVDKARYLYDSLVNFAPIFLAATAASPAFKGWLADQDVRWNVISGAVDDRTPYERSTEPLLPEYNNGNGKSLNEHPMRIPKSRYSAVDLYLGGNSFFDREFNDTDVPVNQEVLERLQSNDIFPMDFDLARHFSHLFIRDPLVVFQERVDQDNKHSTDHFENIQSTNWQTLRFKPPTQEATPENKKCPGWRVEFRPMEIHLTDFENAAYANFIYLVVECLLTFSDDINAYVPMSEVWKNMEIAHRRDAVLDEEFYWKIGFNKEGTRKLSFTEIFHHPESGIFPQFIDRILVHKGFVSSSWTELKNSRQSAHLRLYYYLKLISDRATGALPTTARFLRDFILNHPDYKEDSRLTSVINYDMLKLASRLTHLDDTNGELTRFFGEEIVQFLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGA2</accession>
    <name>C5DGA2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">1-phosphatidylinositol 4-kinase</fullName>
            <ecNumber evidence="3">2.7.1.67</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0D03608g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19877"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58178"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.67"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.67" evidence="3"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552882.1">
        <property type="nucleotide sequence ID" value="XM_002552836.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DGA2">
        <property type="interactions" value="959"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGA2"/>
    <dbReference type="GeneID" id="8295104"/>
    <dbReference type="KEGG" id="lth:KLTH0D03608g"/>
    <dbReference type="eggNOG" id="KOG0902">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000893_1_1_1"/>
    <dbReference type="InParanoid" id="C5DGA2"/>
    <dbReference type="OMA" id="TIEVWQS"/>
    <dbReference type="OrthoDB" id="10264149at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004430">
        <property type="term" value="F:1-phosphatidylinositol 4-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05167">
        <property type="entry name" value="PI4Kc_III_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.70:FF:000011">
        <property type="entry name" value="Phosphatidylinositol 4-kinase alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000022">
        <property type="entry name" value="Phosphatidylinositol 4-kinase stt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1010.10:FF:000014">
        <property type="entry name" value="Phosphatidylinositol 4-kinase STT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.70">
        <property type="entry name" value="Phosphatidylinositol 3-kinase, accessory domain (PIK)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015433">
        <property type="entry name" value="PI3/4_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001263">
        <property type="entry name" value="PI3K_accessory_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042236">
        <property type="entry name" value="PI3K_accessory_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045495">
        <property type="entry name" value="PI4K_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048:SF15">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-KINASE ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00613">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19274">
        <property type="entry name" value="PI4K_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00145">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00915">
        <property type="entry name" value="PI3_4_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51545">
        <property type="entry name" value="PIK_HELICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PIK helical" evidence="9">
        <location>
            <begin position="1334"/>
            <end position="1514"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="8">
        <location>
            <begin position="1601"/>
            <end position="1868"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR22444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1884" mass="213659" checksum="C2A3A6EDDFA73FD9" modified="2009-07-28" version="1">MSAFRESPGSLRALALAKLVNHSEGSRSIDKLVASLPVSYSSHPAKLYTIPLTLNELEVLLGVCQFVPSTAQSAQSLLKKCIIPYFLSNPRQKFTDYVINKYRQRGLKHPTEILSFELSRSIIRISRIFPQFIDEIREQIDEYLQYMSSNLTVENLLSFAGFLEALVYEQNNLLSSFVISVLQAFISEDFLYAVEKTVRNSVSKDVLIDYFDHNREISSLLFCELIEKLQVTYAASLLNVPKKQTLDSYLLDLKHAQFLDGENKALKDFEKKVGQQRSKLLELCKFSFRQITEVEEGAKYIDLSSQNRLESAYTAKSYMLQILSLGLFLSHEVEEVIEMIRDAVIDLPTNSFALKTSLLTTVVCVASLLNYFTDSVSADLLRVFPLMVTTKLITKQKVATISSAVSKGLKPLTEDVIVGTIYNINNLLALAQDGLHMTPVKDRRLTTVLGTSNHFDRLLSSRPTRSNTVGTYQSLQKLKIAETSIVLQHESKLENYTSRQEEVESNSTLYHDSLYENVVTATTTMASVYNDQSITALTITILTQKIKSASPRLDRKILEGLAHLALKVNRSEFNLLMKFFHLSTNSALDNQDEKMMKSLIAAKSIIAEGLAKEDLHNNLFRIYLRYLLDAIVARGDVDRSEHHRSHSEISKVADQIAVYLKPLASLLPKPGEKPLDLIHDEEITNVFRNVWFNAAVHGFHGAAALTSKNHEELLVIAYNSPPLASDFASGNRETSLDMNTILRRGSSNHNLKQQKLILSDHLSMSPVQARTLSSSKVMFLAATLLLEGLRCEAGDCSKTLLYCSDRSIVKSGLDKFVSSISVSMVQRYTKLVIKGDSVIFSAENVAKQLDNILLLLTHRDAPLQDAAYQSCDRFIERIPSSLCHRDSLYTLLDSLTMLFDSVIACEANKHEAVFEFTLKHSNRKMMLPDSLNWRSLTLEKLQKYATRWVTMILKTANQDTKILLQSYISDVSSTRRLNSIEFGVSFALEMAAKILPVDRELANISQGGYRRPDSISGFLSQHAWRSRFIMDQSVLSTYDGIEAERNFSRRKILAHLENKKPLDYKDISAFLDLSASLLILRQGNTASLVYDIVCIPFQIFTSDTIKIATNVWLSVINERSDVGYLLLSEIGHCWRDSIDNNTGLFSMKHNLVAEEFQYMEYSPYNKKEINRRAHAASKSLQPHLQLIRFLSSHFEGTMFDSLHLLHIFTKLCLYGLKNLGKASLHPFARIARSELLYFGTLVFATNVKHNTLYVPEISRFLVDGALTWFEKPKSWPFGANELKIRADLALLIEVYRKLKSQEANLKVHCSKDVSLLEHFLLSEIYSVETWLRPLAKPTDMVKPPTDLVSHAFKKKPELAINLVERYPFKKSSEILTQLVKENPLLCAHSQKTLSFFLAGGKDMHYVLYWAPASPLKSINLFLPQWAQNTFVIQYNVRAIETHDVNLTFFYVPQIVQCLRYDPLGYVERFIIDTAKISVLFSHQIIWNMLANSYKDDDGEIPDDLKPTLDRIHDKMLASFSAEQKDFYEREFSFFNEVTGISGKLRPYIKKTKAEKKIKIDEEMQKISVLDGVYLPSNPDGVVVDIDRSSGKPLQSHAKAPFMATFKIEKTEQDPDTGEDKKVEKWQGAIFKVGDDCRQDVLALQLISLFRTIWSAVGLDVFVFPYRVTATAPGCGVIDVLPNSISRDMLGREAVNGLYEYFTTKFGHENTVEFQHARNNFVKSLAGYSVISELLQFKDRHNGNIMYDDQGHCLHIDFGFIFDIVPGGVKFEAVPFKLTKEMVKVMGGSPDTQAYREFEELCIKAYLAARPHMDLVLECVTPMLGSGLPCFNGNKTIRNLESRFVPHKTDYEAAQHMLSLIKKSYESLFTKGYDEFQRLTNGIPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGE5</accession>
    <name>C5DGE5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Dihydrofolate synthetase</fullName>
            <ecNumber evidence="7">6.3.2.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0D04664g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>7,8-dihydropteroate + L-glutamate + ATP = 7,8-dihydrofolate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23584"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17839"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57451"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Cofactor biosynthesis; tetrahydrofolylpolyglutamate biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the folylpolyglutamate synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.12" evidence="7"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552925.1">
        <property type="nucleotide sequence ID" value="XM_002552879.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGE5"/>
    <dbReference type="FunCoup" id="C5DGE5">
        <property type="interactions" value="181"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGE5"/>
    <dbReference type="GeneID" id="8295151"/>
    <dbReference type="KEGG" id="lth:KLTH0D04664g"/>
    <dbReference type="eggNOG" id="KOG2525">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015869_1_1_1"/>
    <dbReference type="InParanoid" id="C5DGE5"/>
    <dbReference type="OMA" id="NENYLVY"/>
    <dbReference type="OrthoDB" id="5212574at2759"/>
    <dbReference type="UniPathway" id="UPA00850"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008841">
        <property type="term" value="F:dihydrofolate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004326">
        <property type="term" value="F:tetrahydrofolylpolyglutamate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1190.10:FF:000010">
        <property type="entry name" value="Dihydrofolate synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="entry name" value="Mur ligase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="entry name" value="Mur-like, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001645">
        <property type="entry name" value="Folylpolyglutamate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018109">
        <property type="entry name" value="Folylpolyglutamate_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004101">
        <property type="entry name" value="Mur_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01499">
        <property type="entry name" value="folC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11136:SF0">
        <property type="entry name" value="DIHYDROFOLATE SYNTHETASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11136">
        <property type="entry name" value="FOLYLPOLYGLUTAMATE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02875">
        <property type="entry name" value="Mur_ligase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001563">
        <property type="entry name" value="Folylpolyglu_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="MurD-like peptide ligases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="MurD-like peptide ligases, peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01011">
        <property type="entry name" value="FOLYLPOLYGLU_SYNT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mur ligase central" evidence="9">
        <location>
            <begin position="29"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Mur ligase C-terminal" evidence="8">
        <location>
            <begin position="287"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR001563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR22487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="421" mass="46139" checksum="6E11DC161378C656" modified="2009-07-28" version="1">MSIELGLSRITNLLSHLGNPQDKFRVLHVAGTNGKGSVCSLLQSVLQQNTLNLVGKFTSPHLIHITDCISVNNKPISTLDFNSIRTRLDHMNAAHQIGCTEFELLTCTALEYFHLKKCNWCVLEVGLGGRLDATNCTVGSQKVCGITKIGMDHQGFLGNTLQEIAAEKAAIVTSGVPFVAVDGTNDKRVLDIVEKRASLVGATFSPTTTRISGHFIHTDSWGKVAREAVALNGDYQKSNVAVALSMLDFLQREDQVSISREELIDGLKTVSWPGRLQNAELILPQGQQTLKILLDGAHNGDAAEALASYIKEHVRRDSEPLRFLVAVTQGKDLEPLLSPLIRSCDEVVVTKFGPVDRMPWISAMDPSDLARFVSKYTDHVLIQPNLQDAVAYLQNTSPRQTVICGSLYLCGQLLQSTEVKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGH3</accession>
    <name>C5DGH3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">DNA replication regulator SLD2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0D05324g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Has a role in the initiation of DNA replication. Required at S-phase checkpoint.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the SLD2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552953.1">
        <property type="nucleotide sequence ID" value="XM_002552907.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGH3"/>
    <dbReference type="FunCoup" id="C5DGH3">
        <property type="interactions" value="66"/>
    </dbReference>
    <dbReference type="GeneID" id="8295181"/>
    <dbReference type="KEGG" id="lth:KLTH0D05324g"/>
    <dbReference type="eggNOG" id="ENOG502SCF7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057728_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGH3"/>
    <dbReference type="OMA" id="TWEHDFI"/>
    <dbReference type="OrthoDB" id="8775810at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031261">
        <property type="term" value="C:DNA replication preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902977">
        <property type="term" value="P:mitotic DNA replication preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021110">
        <property type="entry name" value="DNA_rep_checkpnt_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040203">
        <property type="entry name" value="Sld2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28124">
        <property type="entry name" value="DNA REPLICATION REGULATOR SLD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28124:SF1">
        <property type="entry name" value="DNA REPLICATION REGULATOR SLD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11719">
        <property type="entry name" value="Drc1-Sld2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4 7" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="88"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="167"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="324"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="101"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="167"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR22515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="381" mass="43472" checksum="25A807AC5129BD8F" modified="2009-07-28" version="1">MFPNLTLLRANTSLDINKPKFHDFSDELTKNIINRLNMDMGQLKLELKRFEKQFQRKYKRLPARDDVRALPEIKEKYKQYSAFQLARQRKDAAPNVGATPRRYEGGTPRRHEEVELGPTPQIYGKAISLFEMKLSPLKPVTVVSPQASSHSPQAVTTEVHQVTTAEIHQFKSQPDLSLTPKRSPKKTTYGPNSPMKFDNVQLSVHTPRRNLRALLDDSVNDTTGSPSPIIKRPPGKPLRQILEEHEKLMEDLEEMSDEEVVRANVKDIFQDDSDATEDEETDEAMLIKKPKRKHILRPAKNALAAQQPFQVNIHEEMERLKQQAASGIDGTIPVKKPASDAKASNPKTRRGNRKYNLVSNNFRRLKLPKAKGKGGKRWGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGI6</accession>
    <name>C5DGI6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0D05610p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0D05610g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552966.1">
        <property type="nucleotide sequence ID" value="XM_002552920.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGI6"/>
    <dbReference type="GeneID" id="8295194"/>
    <dbReference type="KEGG" id="lth:KLTH0D05610g"/>
    <dbReference type="HOGENOM" id="CLU_1156575_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGI6"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="144"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="240" mass="26824" checksum="D40D767744C4F767" modified="2009-07-28" version="1">MFSAYYAFVRLSASLPLFFFHSLQGTLALRTKYNSSFYGSFKLCAVAMPSSLVSVSCLSSFRLRGFLVYGHLYFEFPAPTNSTRCRSWKSSPLVYLIPLGLEVTHARALLAKNGNSRDFAMLKAGIRAQEPEFVREKNVRFVNSYFYVPGAIVIVIYVSTASLEGALRKCWRRASGEYVSTARRLRQLVSLAYKCRDVAGKRESSLLTSGAMTLVFKSGKTRPLTLPKACGAAASWRTRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGJ3</accession>
    <name>C5DGJ3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0D05764p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0D05764g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ISF1/MBR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552973.1">
        <property type="nucleotide sequence ID" value="XM_002552927.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGJ3"/>
    <dbReference type="FunCoup" id="C5DGJ3">
        <property type="interactions" value="32"/>
    </dbReference>
    <dbReference type="GeneID" id="8295201"/>
    <dbReference type="KEGG" id="lth:KLTH0D05764g"/>
    <dbReference type="eggNOG" id="ENOG502SE2X">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068492_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGJ3"/>
    <dbReference type="OMA" id="ENICECE"/>
    <dbReference type="OrthoDB" id="4033526at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031443">
        <property type="entry name" value="Mbr1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17058">
        <property type="entry name" value="MBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="123"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="212"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="157"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="212"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR22535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="274" mass="30022" checksum="E81122915293BDC8" modified="2009-07-28" version="1">MEFSEVKDSKKVSCCSRASDPTENICECEDELDIFERFVQDPCMNAWEDLDEEGRPSQWEDDELDPVCEGVEGFARKQVRTSKPCICSNCHHPRRWKTSSTAQDFDEPVLRCSRTNSFMSQLSRQASRQSLPDPGEGTCGSPTHPGYSQPSLEIGPSRSNSVSRTRSRSSFGASSAIPAHIYCLERFVSSELDSAAENFCRKDPLTLNTVSPTTSTICSPSGSDSATSPQMAGVLSSSSSTNSAKNNADIQPVFLSKRKSRVSSIEVSLLNSFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGL1</accession>
    <name>C5DGL1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0D06204p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0D06204g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic ribosomal protein eL14 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552991.1">
        <property type="nucleotide sequence ID" value="XM_002552945.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGL1"/>
    <dbReference type="FunCoup" id="C5DGL1">
        <property type="interactions" value="1210"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGL1"/>
    <dbReference type="GeneID" id="8295221"/>
    <dbReference type="KEGG" id="lth:KLTH0D06204g"/>
    <dbReference type="eggNOG" id="KOG3421">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082438_3_1_1"/>
    <dbReference type="InParanoid" id="C5DGL1"/>
    <dbReference type="OMA" id="KLCFVVD"/>
    <dbReference type="OrthoDB" id="1875589at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000470">
        <property type="term" value="P:maturation of LSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23702">
        <property type="entry name" value="eL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000030">
        <property type="entry name" value="60S ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039660">
        <property type="entry name" value="Ribosomal_eL14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002784">
        <property type="entry name" value="Ribosomal_eL14_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11127">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11127:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01929">
        <property type="entry name" value="Ribosomal_L14e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="KOW" evidence="7">
        <location>
            <begin position="17"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein eL14" evidence="8">
        <location>
            <begin position="54"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR22553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="138" mass="15216" checksum="53E5083C2B500D52" modified="2009-07-28" version="1">MSSESIVKNSQWRLVEVGRVVLIKKGPSAGKLAAIVEIIDQKRALIDGPETNVPRQSVNLGHVVLTPLTFALPRGAKTAVVAKKWTSAGVAEKWAASSWAKKIAQRERRAALSDFERFQVMVLRKQRRYAVKKAVAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGQ0</accession>
    <name>C5DGQ0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0D07106p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0D07106g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553030.1">
        <property type="nucleotide sequence ID" value="XM_002552984.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGQ0"/>
    <dbReference type="FunCoup" id="C5DGQ0">
        <property type="interactions" value="654"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGQ0"/>
    <dbReference type="GeneID" id="8295261"/>
    <dbReference type="KEGG" id="lth:KLTH0D07106g"/>
    <dbReference type="eggNOG" id="KOG4524">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005544_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGQ0"/>
    <dbReference type="OMA" id="PHPKKPW"/>
    <dbReference type="OrthoDB" id="6781668at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052587">
        <property type="entry name" value="TELO2-interacting_protein_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059075">
        <property type="entry name" value="TPR_TTI1_2nd_yeast"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057567">
        <property type="entry name" value="TPR_TTI1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057566">
        <property type="entry name" value="TPR_TTI1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016441">
        <property type="entry name" value="Tti1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049362">
        <property type="entry name" value="TTI1_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18460">
        <property type="entry name" value="TEL2 INTERACTING PROTEIN 1 TTI1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18460:SF3">
        <property type="entry name" value="TELO2-INTERACTING PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26245">
        <property type="entry name" value="TPR_TTI1_2nd_yeast"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24181">
        <property type="entry name" value="TPR_TTI1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24173">
        <property type="entry name" value="TPR_TTI1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21547">
        <property type="entry name" value="TTI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005250">
        <property type="entry name" value="UCP005250"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TTI1 N-terminal TPR" evidence="2">
        <location>
            <begin position="9"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="TEL2-interacting protein 1 second TPR" evidence="4">
        <location>
            <begin position="380"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="domain" description="TTI1 C-terminal TPR" evidence="3">
        <location>
            <begin position="816"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="818"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="819"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="830"/>
            <end position="839"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF26245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR22592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1042" mass="116517" checksum="9859F12AABE37DF4" modified="2009-07-28" version="1">MASRESVAFQRVQPHCVNLSRQSFLPRDVFNPNSVVLLEGLRSLEEELKQLENEGVSLSPKFADYVFVPIASLLRQDSLGSSQTEALLQVIGHLVSLCWSSAGSFNKALAQQLFALVTFLISNDKDNTCLLTKTPSFQLTGCIVLRKLFKAFAIQKASGSFDHFADPQNLPSLGHSVTILLDILGGNDNEIDLQLAAIEALHVLYQETLNDGEIISFIVPGNVSAFSKLLLKPGLTVNPRVVCSTLRLFAVIICIVYSDFDLHVVEHSPSDLNELLEEAEKPFETNIAINEVDFKNRNLHRDIKWLRATSAQINRALKGVLPKLVKRDNGSINLALCEFISALMKHCRASLNNCQKLFFSVALELHYVPGELVQTLEGTRTLKLMVDNKVTRISNAIQFEDIKALQTIQYGLQTLYSYQCLDFEMVDGLVFSLKSCVSDFLEQKNLRFTEGKLKEQGGMVIVGQDFLTLTETSQPLLPKISTALEETLSQLLHTLGTCYSRDHIQGTVSELLTFDGSESGFGKAVSLWMSANLLKAASEVLPDAHDITDEFMTFENDERKVADLPLEPIETCYAILESSVALIDEVNQNSAALHHDSEFQVVVGLKSIQIMSETLGEEFRTELIDILFPVVDCLASPSSAIRHFAQTTTLSIAKSFYGGSIHDLLLDNIDYLVDAISVRLNNCMTKRVPTLLMVLCRVGGYNLIKSFKDVLEIIFRLLDFYHGYEDLCCEFFQLFEVIVTEMRHTYLSSTDVSPRLKNEHSVSSSFKPWGMQNILQVLDILDKTGEKGIHEEDFDDLDSNQDDPGTFQEFFDKRVHEVDSDDEDEDIQPTEGQSTSSGHASDKWFSPIPRDSYRLLLQIVGYGDRLLTHPSRQLKVQILQLMSRVFPMLATQHDMLLPQVSKSWDIIVQLSLDPDFAIAKVACECLRELIHCSGDFVTKRFVDLWGAQKAHSPLLGIAAPKGVAPTLSSKKWLTSPKQFPPITREALVSLSEMLLEGIVQTELFTSEVQIQEMVYCCLLVLPKEHVQSRSLLLGDIVSLLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGW8</accession>
    <name>C5DGW8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Chromatin modification-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0D08910g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of an histone acetyltransferase complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of an histone acetyltransferase complex. Interacts with H3K4me3 and to a lesser extent with H3K4me2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">The PHD-type zinc finger mediates the binding to H3K4me3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the ING family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553098.1">
        <property type="nucleotide sequence ID" value="XM_002553052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGW8"/>
    <dbReference type="FunCoup" id="C5DGW8">
        <property type="interactions" value="345"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGW8"/>
    <dbReference type="GeneID" id="8295335"/>
    <dbReference type="KEGG" id="lth:KLTH0D08910g"/>
    <dbReference type="eggNOG" id="KOG1973">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031900_2_1_1"/>
    <dbReference type="InParanoid" id="C5DGW8"/>
    <dbReference type="OMA" id="HEIDAKC"/>
    <dbReference type="OrthoDB" id="5411773at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033698">
        <property type="term" value="C:Rpd3L complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070210">
        <property type="term" value="C:Rpd3L-Expanded complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17016">
        <property type="entry name" value="ING_Pho23p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15505">
        <property type="entry name" value="PHD_ING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000016">
        <property type="entry name" value="Inhibitor of growth protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028651">
        <property type="entry name" value="ING_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024610">
        <property type="entry name" value="ING_N_histone-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10333">
        <property type="entry name" value="INHIBITOR OF GROWTH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10333:SF42">
        <property type="entry name" value="INHIBITOR OF GROWTH PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12998">
        <property type="entry name" value="ING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01408">
        <property type="entry name" value="ING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3 9 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="13">
        <location>
            <begin position="274"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="137"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="214"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="279"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="301"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="317"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="320"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="8">
        <location>
            <position position="276"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="8">
        <location>
            <position position="287"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="8">
        <location>
            <position position="291"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="8">
        <location>
            <position position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR628651-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR628651-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR22660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="324" mass="33956" checksum="9DB1F6A7F7908A14" modified="2009-07-28" version="1">MAAPANLYPGLNDISDVLEEMPLEVTRYLTLLREIDAKCVHTVPELNSTIGEFLGARGSKNDGVEPLARMNGLFQDLMPSLEEKMHVSALAYEALNRLTARLELAYEVAIKNNEIPPKLRLGNDNHPAMHLHHELMAKIDSKSGSKSSQALKSESRREAMAARRTAGAPPSVPAGHATGNGGGAGGAGAGGAGAGGFAAGAAASGSAAAATAAGAAQTTDGRKRRTMAGPTGTASGAMGATAGSASADSKKRKRKTASREDYSQRPKTNEYGEALYCYCNQVAYGEMVGCDGDNCILEWFHLPCIGLETLPKGKWYCDDCKRAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DH49</accession>
    <name>C5DH49_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Adenosine kinase</fullName>
            <shortName evidence="11">AK</shortName>
            <ecNumber evidence="4 11">2.7.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Adenosine 5'-phosphotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0E01342g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>adenosine + ATP = AMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20824"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16335"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenosine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the carbohydrate kinase PfkB family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.20" evidence="4 11"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553547.1">
        <property type="nucleotide sequence ID" value="XM_002553501.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DH49"/>
    <dbReference type="FunCoup" id="C5DH49">
        <property type="interactions" value="1349"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DH49"/>
    <dbReference type="GeneID" id="8291685"/>
    <dbReference type="KEGG" id="lth:KLTH0E01342g"/>
    <dbReference type="eggNOG" id="KOG2854">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045832_1_0_1"/>
    <dbReference type="InParanoid" id="C5DH49"/>
    <dbReference type="OMA" id="RTMCTYL"/>
    <dbReference type="OrthoDB" id="432447at2759"/>
    <dbReference type="UniPathway" id="UPA00588">
        <property type="reaction ID" value="UER00659"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004001">
        <property type="term" value="F:adenosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044209">
        <property type="term" value="P:AMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006144">
        <property type="term" value="P:purine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01168">
        <property type="entry name" value="adenosine_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001805">
        <property type="entry name" value="Adenokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002173">
        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45769">
        <property type="entry name" value="ADENOSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45769:SF3">
        <property type="entry name" value="ADENOSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00989">
        <property type="entry name" value="ADENOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00584">
        <property type="entry name" value="PFKB_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 11" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase PfkB" evidence="12">
        <location>
            <begin position="36"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601805-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR23110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="341" mass="36034" checksum="AE9D09F20E5EAE1B" modified="2009-07-28" version="1">MSASPKLVCLGNPLLDIQATVDEAYLAKYSLKENDAILVDASSNEPKMAIFDELLQFPDVKFIAGGAAQNTARGAAYVLGAGKVGYFGSVGQDKFSSKLLAENEAAGVVSYYQEQSKVGTGKCAALITGHNRSLVTDLGAANHFTPDHLDKHWDVVEKAELFYIGGFHLTVSPGAIVKLGKHAQATGKPFVLNLSAPFIPQFFKSALEQVLPYTTYVIGNESEAAAYAEAFGLDCKQDDLASIAKHVLGGSTTRTVIFTHGLEPTVIVSANDVATRPVKALPKDSIVDTNGAGDAFAAGFMAGLTQGKNLDQSVDIGQWLAALSIQEVGASYPAEKLQYQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHA1</accession>
    <name>C5DHA1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0E02596p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0E02596g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553599.1">
        <property type="nucleotide sequence ID" value="XM_002553553.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHA1"/>
    <dbReference type="FunCoup" id="C5DHA1">
        <property type="interactions" value="17"/>
    </dbReference>
    <dbReference type="MEROPS" id="A01.097"/>
    <dbReference type="GeneID" id="8291742"/>
    <dbReference type="KEGG" id="lth:KLTH0E02596g"/>
    <dbReference type="eggNOG" id="KOG1339">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023427_0_0_1"/>
    <dbReference type="InParanoid" id="C5DHA1"/>
    <dbReference type="OMA" id="KNIYMAM"/>
    <dbReference type="OrthoDB" id="771136at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF51">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950477" evidence="3">
        <location>
            <begin position="17"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="4">
        <location>
            <begin position="42"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="438"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="443"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR23162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="596" mass="64614" checksum="C6C9699FD27E6930" modified="2009-07-28" version="1">MLIFAVFVFAVALVRGASQSSSENSIDSFPVLTANRDRAGLYYVNASFGTPGQSQKLRIDLVQPYMWVFSEDMRQARQNRSARAKAETYYNASKSSTHEKLNGDKIWSMHFMDHIAFNGTTVMDEVSFMWSSTSSSSDVATGDSNTTQDFSSANTSRISDKSFSFLSANEVAAYIQQGALGLAGRAAQTGYDNDSSDFDSSYVLLDRLVDESLIYTSSFSMWMGADTRDPLESPEPSNGCGKVIFGAVDTSLYTGDFVKFDTIPFHNARTGETSRGYPIIPLTKVNVQSKSGETKNMTSDSFLEPVLIDSRFRYNYLPLEIIVQIAMQANAYYVESLNRWLLACDVGRLGASIIFEFGNLSINVPLNNLMGPTFDSDTNGTLHFSNKQQACELKLLPSTASGFNVLGGAFIKNVYMAAELESNQVALAQAQKVSSVTTTSSTAELSPTPTSSKSEEGGDDAASHLSYFNLKPAKTISAIKSGMIPFATTNNLTSFQTMMLSDKATMSTSPNTRLFNQFTATISSDGVIFTGRSFYNTTNDVSVSSTTSASFALSETTSKSNAARTNFPLQSVGGSGLSMHFYSVTVLLISAAIFAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHC8</accession>
    <name>C5DHC8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0E03256p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0E03256g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553626.1">
        <property type="nucleotide sequence ID" value="XM_002553580.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHC8"/>
    <dbReference type="GeneID" id="8291771"/>
    <dbReference type="KEGG" id="lth:KLTH0E03256g"/>
    <dbReference type="eggNOG" id="ENOG502RZ2S">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032419_0_0_1"/>
    <dbReference type="InParanoid" id="C5DHC8"/>
    <dbReference type="OMA" id="EWHDELR"/>
    <dbReference type="OrthoDB" id="3266505at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051127">
        <property type="entry name" value="Fungal_SecMet_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47424:SF6">
        <property type="entry name" value="PROLINE UTILIZATION TRANS-ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47424">
        <property type="entry name" value="REGULATORY PROTEIN GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="8">
        <location>
            <begin position="16"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="570"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="55"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR23189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="674" mass="75455" checksum="C299021063750D43" modified="2009-07-28" version="1">MPPEKVAKERKRSKKACQRCSAQKVKCLGEKPPCRACVLSKNQDNCIFPVKPRKITVLDTDVYQMEQKLQKLQSECSRLKGLAAAATCEPSEEAEDLDPLYHGPTICTDSPGKSSGSNVAGSPSCQIFIGNLQWRILGDATKQKFDERATNNNTSMAAEEGRNCLKRIHLNDRVINTKEAFEAPVLPLKAYALEMVHTVFNFFAREYGLFSKAGFEARLNETYKHPNSQDSDWLAYLMITFAVGEQYMNEAGLKNQIPGMCYYISALKYFHEPIEEPNLDSVRTLILIAFYSQGLNRMNSVYAYTGLALSTAVAQGIHRQANNHQFPRAEQEARKKIWWTAFLMDTLWASRLGLPPHFNSCDIDIELPDESMSTEDGFDPKYLIANTHLALLVRSVMREVYGATRESFVENVFCNLKRLEIFMASLKPELKTYTTVAKDNRSTANLHLRYNQLIILTVRPLYLSLLKDPKKRTEEIDRAIIKCTEAAAANVGILCTLFESKCFSAFGFLEAQCCFSSILILIMAVLDGHSFSELPVAVSLNSFMSNIGNITAIDNHCRLRTLIEVLPRSQNHSTTATAKSSVQSSAKPNSKTTPPAETNSRIKSTVTEFSNKFHVQDTCSTATCQSGKWPLDPELEFLGNLDECSNGFEQLTPETASNISSNLGKWDSFISKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHD8</accession>
    <name>C5DHD8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0E03564p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0E03564g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553636.1">
        <property type="nucleotide sequence ID" value="XM_002553590.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHD8"/>
    <dbReference type="FunCoup" id="C5DHD8">
        <property type="interactions" value="72"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHD8"/>
    <dbReference type="GeneID" id="8291785"/>
    <dbReference type="KEGG" id="lth:KLTH0E03564g"/>
    <dbReference type="eggNOG" id="KOG2422">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008321_1_1_1"/>
    <dbReference type="InParanoid" id="C5DHD8"/>
    <dbReference type="OMA" id="LEDPLGC"/>
    <dbReference type="OrthoDB" id="205993at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990112">
        <property type="term" value="C:RQC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990116">
        <property type="term" value="P:ribosome-associated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006994">
        <property type="entry name" value="TCF25/Rqc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22684">
        <property type="entry name" value="NULP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22684:SF0">
        <property type="entry name" value="RIBOSOME QUALITY CONTROL COMPLEX SUBUNIT TCF25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04910">
        <property type="entry name" value="Tcf25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="710" mass="81119" checksum="4CA517F329B371D3" modified="2009-07-28" version="1">MSSRALRRLEENDLEKTLAKLAPSTQKQEPSAKTSSVKAPKQINLFALMGQDEGDDSEDSEASNAEDRPIPASINDKPAQKPVQVLTKSQRKKNKKNKKSKQKPKPAADLSETENGNGESDEELDRMILQFQKKDLEQRRSVDPGLTASDESDYDTAFEDEDASHLKGPADVKTDPGFTNFTSFSEIIKIFGDIDMKNLNPDNEYKLLFGDLSPESLADVDSMTSTHVSPQVMKQIERLKHMVRNWGGRDHKTVPNGSTTRRLAFTKIREDWIPTPRGEYTITQLSQDEVVEWQSWLRSQDWMDEVRKTAHKWKDAGMNFYKFEPSNTETNRKAMTEFYMSVVLHPDHEALIHLISSKFPYHVPALLQVALILVRQGDKANSNGLVERALFVFDRALRSHISFNGLSCQLPYIYFFNRQFYFAIFRYVQILSQRGVVSTASEWCKVLWSLSPLEDPLGCRYFIDHYLLLNKEYSYMANLASSPLVNTYMQWYTLGLSLGFVLSFLKLDMVEKAKTELRKAFTYHAASLSTIFKEVALGDANLLNGLDFPEPCSEIELESKAYLIRMKAIWGGVEIKFLHDEILNILSEYKAGKIQLECTAPIETQNELFLGDIPINLLRFAVLSQESPLMACIPQEIWSERDVYEFDVLPPLPHDRESEDLVETVKTFVSESDLSMSQMEVMQDENMLNQIRQLSLEQFLQENPNADLGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHG9</accession>
    <name>C5DHG9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0E04246p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0E04246g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553667.1">
        <property type="nucleotide sequence ID" value="XM_002553621.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHG9"/>
    <dbReference type="FunCoup" id="C5DHG9">
        <property type="interactions" value="201"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHG9"/>
    <dbReference type="GeneID" id="8291816"/>
    <dbReference type="KEGG" id="lth:KLTH0E04246g"/>
    <dbReference type="eggNOG" id="KOG1946">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046065_1_0_1"/>
    <dbReference type="InParanoid" id="C5DHG9"/>
    <dbReference type="OMA" id="TMFALNK"/>
    <dbReference type="OrthoDB" id="10251073at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="CDD" id="cd10567">
        <property type="entry name" value="SWIB-MDM2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.245.10">
        <property type="entry name" value="SWIB/MDM2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014876">
        <property type="entry name" value="DEK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019835">
        <property type="entry name" value="SWIB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036885">
        <property type="entry name" value="SWIB_MDM2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003121">
        <property type="entry name" value="SWIB_MDM2_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13844">
        <property type="entry name" value="SWI/SNF-RELATED MATRIX-ASSOCIATED ACTIN-DEPENDENT REGULATOR OF CHROMATIN SUBFAMILY D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08766">
        <property type="entry name" value="DEK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02201">
        <property type="entry name" value="SWIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00151">
        <property type="entry name" value="SWIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47592">
        <property type="entry name" value="SWIB/MDM2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51998">
        <property type="entry name" value="DEK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51925">
        <property type="entry name" value="SWIB_MDM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEK-C" evidence="3">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="DM2" evidence="2">
        <location>
            <begin position="111"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="187"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR23230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="209" mass="23812" checksum="A7650DF6D36D84F6" modified="2009-07-28" version="1">MAELDTYISMIDAILGVSDPDEVSAKRIRRAIQELFAVDLEPERKKVNALIIERFNRLQDARAEVSQDELVEDDAKVARTLAKKVNGKAKKDSRNTKVKKKRKVSESSNSLNQKKMQLSDELQKLVGEPEMARTQVVKKVWEHIKEQDLQNPQDRREILCDELMKPIFGDKTTMFALNKSLSKHIYSRDKKVQATEPAGSPFTTEVSTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHP2</accession>
    <name>C5DHP2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0E05940p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0E05940g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553740.1">
        <property type="nucleotide sequence ID" value="XM_002553694.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHP2"/>
    <dbReference type="FunCoup" id="C5DHP2">
        <property type="interactions" value="62"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHP2"/>
    <dbReference type="GeneID" id="8291893"/>
    <dbReference type="KEGG" id="lth:KLTH0E05940g"/>
    <dbReference type="eggNOG" id="KOG4157">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024893_2_1_1"/>
    <dbReference type="InParanoid" id="C5DHP2"/>
    <dbReference type="OMA" id="CPGYKDQ"/>
    <dbReference type="OrthoDB" id="2537459at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002889">
        <property type="entry name" value="WSC_carb-bd"/>
    </dbReference>
    <dbReference type="SMART" id="SM00321">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51212">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950376" evidence="3">
        <location>
            <begin position="29"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="411"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="WSC" evidence="4">
        <location>
            <begin position="27"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="181"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="381"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="489"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="181"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="381"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="494"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR23303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="556" mass="56482" checksum="B38928B91337F718" modified="2009-07-28" version="1">MNVRSRSPSRLAWSCVLLWACVSSFAAAAQTYCSSENTASTAAKWWQYQSNGWCSDECAGYSYAVLQGHNCWCSNTEPQSTTSVSDCSDTCPGYHEQCGNVDEGLFGYIYLGEASSTSSASSTVSSTASSVSSTASSVSSTASSTASSVSSAASSTASSVSSAASSTASSVSSAASSTSSSIITSSSTSSSTSSSTSSTSTSSSTSSEATSSATSPASSASSSSSSSSPPTSSEPSSTSSTTPTASSAAASASASTSASSSATPSTSSSVTSTPLTTSSSSTSQTSSSSSTLQTTSSSSTPQASSSAALSSTSPPSSSLKPTSSSRSSPRSSSTSFSQNMVTSILYSVQTVTASPAAGGTSDDRVFTTQIMTTVYATPSGQLPSLSSSSSNANANSRNSGGSGFWGSPGKVAGTFVAVGVVVFCLALLGFILWYRRRRRNEEDFEKRYNDVTAPAGSNTSIDPASAGSHAAFVYADEKGIEVPPTPVEKKETFTRLSQPASFTDNESGIPQDEGPVVVDQRLDPRQVFMHWENEGSKTSLADDVDYSRRVLRVINE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHW7</accession>
    <name>C5DHW7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 18">3-isopropylmalate dehydratase</fullName>
            <ecNumber evidence="6 18">4.2.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16 18">Alpha-IPM isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17 18">Isopropylmalate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ordered locus">KLTH0E07722g</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 18">Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 18">
            <text>(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate</text>
            <dbReference type="Rhea" id="RHEA:32287"/>
            <dbReference type="ChEBI" id="CHEBI:1178"/>
            <dbReference type="ChEBI" id="CHEBI:35121"/>
            <dbReference type="EC" id="4.2.1.33"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 18">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 18">Belongs to the aconitase/IPM isomerase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.33" evidence="6 18"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553815.1">
        <property type="nucleotide sequence ID" value="XM_002553769.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHW7"/>
    <dbReference type="FunCoup" id="C5DHW7">
        <property type="interactions" value="275"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHW7"/>
    <dbReference type="GeneID" id="8291972"/>
    <dbReference type="KEGG" id="lth:KLTH0E07722g"/>
    <dbReference type="eggNOG" id="KOG0454">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006714_0_1_1"/>
    <dbReference type="InParanoid" id="C5DHW7"/>
    <dbReference type="OMA" id="EDNEPHT"/>
    <dbReference type="OrthoDB" id="2279155at2759"/>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00071"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009316">
        <property type="term" value="C:3-isopropylmalate dehydratase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003861">
        <property type="term" value="F:3-isopropylmalate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01583">
        <property type="entry name" value="IPMI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01577">
        <property type="entry name" value="IPMI_Swivel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.499.10:FF:000006">
        <property type="entry name" value="3-isopropylmalate dehydratase large subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.499.10:FF:000007">
        <property type="entry name" value="3-isopropylmalate dehydratase large subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.19.10:FF:000003">
        <property type="entry name" value="3-isopropylmalate dehydratase small subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="entry name" value="Aconitase, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="entry name" value="Aconitase, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01026">
        <property type="entry name" value="LeuC_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01031">
        <property type="entry name" value="LeuD_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004430">
        <property type="entry name" value="3-IsopropMal_deHydase_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004431">
        <property type="entry name" value="3-IsopropMal_deHydase_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012235">
        <property type="entry name" value="3-IsopropMal_deHydtase_ssu/lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050067">
        <property type="entry name" value="IPM_dehydratase_rel_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033941">
        <property type="entry name" value="IPMI_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033940">
        <property type="entry name" value="IPMI_Swivel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00170">
        <property type="entry name" value="leuC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00171">
        <property type="entry name" value="leuD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002458">
        <property type="entry name" value="PRK01641.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004016">
        <property type="entry name" value="PRK05478.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009116">
        <property type="entry name" value="PRK12466.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43822:SF9">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43822">
        <property type="entry name" value="HOMOACONITASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001418">
        <property type="entry name" value="ACN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="Aconitase iron-sulfur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52016">
        <property type="entry name" value="LeuD/IlvD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01244">
        <property type="entry name" value="ACONITASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="10 18" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="15 18" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0408">Iron</keyword>
    <keyword evidence="13" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8 18" id="KW-0432">Leucine biosynthesis</keyword>
    <keyword evidence="14 18" id="KW-0456">Lyase</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha" evidence="19">
        <location>
            <begin position="14"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="Aconitase A/isopropylmalate dehydratase small subunit swivel" evidence="20">
        <location>
            <begin position="553"/>
            <end position="680"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00014371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00031631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00033368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRNR" id="PIRNR001418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="CAR23378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="783" mass="86167" checksum="4B5741F69109E848" modified="2009-07-28" version="1">MTYTPSKGPKTLYDKVFDAHVVHKDESGSYLLYIDRHLVHEVTSPQAFEGLKDADRKVRRRDCTLATVDHNIPTWSRKNFKSPETFIKEADSLLQVQTLQQNVKDFDVPFFGMTDARQGIVHIIGPEQGFTLPGTTVVCGDSHTSTHGAFGSLAFGIGTSEVEHVLATQTIIQAKSKNMRIFVEGKLSPGVTSKDLILHVIGVIGTAGGTGCVIEFAGEAVEELSMEARMSICNMAIEAGARAGMIKPDQITFDYVKGRPLAPKEQEWEKAVAYWNTLHTDEGAQFDYDIKIKSKDIIPTITWGTSPQDALPITASVPDPTNVSDPIKKSMMEGALKYMGLKPNTPLKEISIDKVFIGSCTNARIEDLRAAAAVVKGHKKAESVKLAMVVPGSGLVKQQAEKEGLDRVFEEAGFEWREAGCSMCLGMNPDILDPEERCASTSNRNFEGRQGARSRTHLMSPAMAAAAGIEGRFVDIREFQYKDNDVPKVSIEQETEDKALQDAVYEHEKQPLPEGESVQEQKDDEVKDIPATNHTIHESPIKNGKASSAPAGMEPFLTVSGVAAPVDKANVDTDAIIPKQFLKTIKRTGLKKGLFYEWRFTKDENGNEVSLNYILDTEPYAKSKILVVTGDNFGCGSSREHAPWALKDFGIKSIIAPSFGDIFYNNSFKNGLLPIRLPQEVILEKLYPVAQSAGSLVIDLPNQQILDSNNNVLVEHFDVEDFRKHCLVNGLDDIGITLQKEELISKYEAKRKVEFSFLEGGSGGVRPIVGSKKSPYGNTAQEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHY5</accession>
    <name>C5DHY5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0E08162p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0E08162g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat CDC20/Fizzy family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553833.1">
        <property type="nucleotide sequence ID" value="XM_002553787.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHY5"/>
    <dbReference type="FunCoup" id="C5DHY5">
        <property type="interactions" value="851"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHY5"/>
    <dbReference type="GeneID" id="8291992"/>
    <dbReference type="KEGG" id="lth:KLTH0E08162g"/>
    <dbReference type="eggNOG" id="KOG0305">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014831_4_1_1"/>
    <dbReference type="InParanoid" id="C5DHY5"/>
    <dbReference type="OMA" id="WSKNTDE"/>
    <dbReference type="OrthoDB" id="10263272at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010997">
        <property type="term" value="F:anaphase-promoting complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990757">
        <property type="term" value="F:ubiquitin ligase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905786">
        <property type="term" value="P:positive regulation of anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033010">
        <property type="entry name" value="Cdc20/Fizzy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056150">
        <property type="entry name" value="WD40_CDC20-Fz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918">
        <property type="entry name" value="CELL DIVISION CYCLE 20 CDC20 FIZZY -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918:SF1">
        <property type="entry name" value="FIZZY-RELATED PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24807">
        <property type="entry name" value="WD40_CDC20-Fz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="CDC20/Fizzy WD40" evidence="7">
        <location>
            <begin position="245"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="371"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="413"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="501"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="137"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="35"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="158"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF24807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR23396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="556" mass="61546" checksum="289BF9D5E7A6112F" modified="2009-07-28" version="1">MSEEMRNPFISNTPSSSPAKSRVQSGNRITKRDTSSSSSLTSVNSSPLRRPRSSNGGYTDRFLPNRTNMDLKSVISIGSSQLPRLVPSDSDNHLEVRREQEAHETFDAVLKNELFGEKLCEASNENEDSIERIKYTATFPYREPSTPPRSQEASGRDALLSPSPSSPATPRRLTFENHSKPSSSSMRGASLLTYCEKKASRPSAASLLQSQYSSSASPVRPDSKKLLLSPSKKVRQISKIPFRVLDAPSLADDFYYDLVDWSSADMLAVALGKSIFLTNNNTNEVAQLAKTDDDYTSLSWVGAGSHLAVGQANGLVKIFDVEKKRCIRTIPGHIDRVACLSWNNHILTSGSRDRRILHRDVRTPEPCFEEIRTHKQEVCGLKWNVDENQLASGGNDNVVFVYDGTSRKPILTLAEHTAAVKAMAWSPHRRGVLATGGGTADKRLKIWNVRTSTKLHDVDTASQVCNMIWSRNTNEIITSHGYSKYNLTLWDGVNAEPIAILKGHSFRVLHMTLSADGTTIVSGAGDETLRYWKLFDKPRPQAHAASALLSTFNQLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHZ8</accession>
    <name>C5DHZ8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0E08514p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0E08514g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553846.1">
        <property type="nucleotide sequence ID" value="XM_002553800.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DHZ8">
        <property type="interactions" value="578"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHZ8"/>
    <dbReference type="GeneID" id="8292007"/>
    <dbReference type="KEGG" id="lth:KLTH0E08514g"/>
    <dbReference type="eggNOG" id="ENOG502R0S3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_251473_0_0_1"/>
    <dbReference type="InParanoid" id="C5DHZ8"/>
    <dbReference type="OMA" id="IHFYQIA"/>
    <dbReference type="OrthoDB" id="4068315at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035361">
        <property type="term" value="C:Cul8-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031297">
        <property type="term" value="P:replication fork processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019021">
        <property type="entry name" value="Mms22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28122">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SUBSTRATE RECEPTOR MMS22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28122:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SUBSTRATE RECEPTOR MMS22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09462">
        <property type="entry name" value="Mus7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="219"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="306"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="382"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="346"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="358"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1448" mass="166250" checksum="32EEB178E0118E05" modified="2009-07-28" version="1">MDEDTNLTYIADSEAEPDEYLLWKPARPINYGEDGQIDQHNNDSGPMPEPLAFQQTEGSDARYQSPIQRVRSLRKRTAIQKKPYSLDRIKHRHLLRGFGISNEDELPSSLQDHRDLQYNDEASQGGYFMNDDKSETECQNVESFEVISSEHQRFNSNSNGVVTSEAQSRLKEERIPENSAVKTATPANHSIIYRGRQVNVNSGFRGILPKVAWTKALNDSRQSKRQLPKQERRSGKGVAKRKKQKVNQRQDDSLFGDLFIADDNFSAEDNLPHNIVDLSDSPEEKPELISKYLDSRYSEAYAFDDLSEPDVEGPDNFGGLDGPSTISADSKRSKRSKRPRQPSTSKLDKSGDILQRESTYSVSSGDSSSAEEWDDHTIDTMLRARTRKTGGSTKAAKSQRVKRPYEIVANRRITYRRSGTMTANPQHNESHYSIENGKPGAFPKYRRPPHQKLIEFPGKAKISRRPEELDWYREQHAISSTYPSWQKNSASKSTLSSGNQNLNSLVSRAGKLFFETAIEQESDRFTLVPSSKKKSSTINLEAAHSPKNPDNKPNSDVQVFDAIIFDSLIHPPDTIHFTLSEKAFKISRFDTNFENSLSEVFNHLIEVGAIDTEVTIMNSSLVAVLYHLNKPGLWELVNEFHNKFRSKVNLLRSRAKAIHFYQISVCQVMLLEIAGYSSVSRLLASEVTKKIIDHTVSFFRLLSKCYNLVANSNLMEQCYILLAKVVEKTLLKSELWDKLRNTLFPPKVALILVKYFPTRETFWEIVELGHTFESVVDWFKFVHFSVGACGWEISHRLILVIYNFFKTRKFQDFEEEEDRWSEYPIFSQNELKQSRRTAFNSFLGLLASVRLPMKIIERITPLGQIISLQSPGLLANRINLLLVLADQAPTSFEIKLEDLCYPYLHENSADPVSPVMLDLILKGFISILQINSKKNLPLRARFISLIWQSVLKRHEARIEKAWLNFLRSLCSIFYKLKKSESVILKALHPILMSTLPNKKNQRDGVGLIKLFEDNLQKLEPAWVARNLHQILASSAGDSERIFSFYFNITEYLASRKVITWWSVLKYNNFNHSDDYKLLFYTRAIEKCDDSTFLQIRHVLFQTVTDLLLQRSDVPFIKFIRSISKRDRLFKINPELPFTASHLEIITKVLTGFKRSNDRIVLKVVICKLKELLLGHPEKKPLISEVTKFLNKEYVDLVKNDPDFLFLKNQFNISDEETQKSIFREALSMRKDDIERSLHIEREILRIGSRGQNLEHFLGMLESSMGTGLYVNDFLFLSQLLEANAFSEGLERTSTQWFVLSNLILMINNILKQNFCQVSSEDFFGLYHLHYWICRAFSLKEFELDAISDKSVFLREACVLQTRTLLMSSGFLEHNSLLSLSEEFLTKPRPTRFEPKAALTGPVHSLLKEYGPFIMSLAPSREDIQAFQGDVATQLSKLSFLVKSKVSRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5DI22</accession>
    <name>C5DI22_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0E09086p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0E09086g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553870.1">
        <property type="nucleotide sequence ID" value="XM_002553824.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DI22"/>
    <dbReference type="FunCoup" id="C5DI22">
        <property type="interactions" value="40"/>
    </dbReference>
    <dbReference type="GeneID" id="8292033"/>
    <dbReference type="KEGG" id="lth:KLTH0E09086g"/>
    <dbReference type="eggNOG" id="ENOG502S6V3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068499_1_0_1"/>
    <dbReference type="InParanoid" id="C5DI22"/>
    <dbReference type="OMA" id="VKLWEDI"/>
    <dbReference type="OrthoDB" id="9626941at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070072">
        <property type="term" value="P:vacuolar proton-transporting V-type ATPase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013945">
        <property type="entry name" value="Pkr1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28251">
        <property type="entry name" value="V-TYPE ATPASE ASSEMBLY FACTOR PKR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28251:SF1">
        <property type="entry name" value="V-TYPE ATPASE ASSEMBLY FACTOR PKR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08636">
        <property type="entry name" value="Pkr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR23433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="119" mass="13607" checksum="7D3628943E6962AE" modified="2009-07-28" version="1">MANFFEELWRSIFTPGASPQLIVATHVSFFLLTACLSWLIFWTKNIHFIMLLIISTLLWGTVTWFISELKSAQMKDNEELVKSQEKKTDGAKPQEESEKSQPKGQSSAVQTKKAKSRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>C5DI71</accession>
    <name>C5DI71_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Histone H2B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0E10186g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the histone H2B family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553919.1">
        <property type="nucleotide sequence ID" value="XM_002553873.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DI71"/>
    <dbReference type="FunCoup" id="C5DI71">
        <property type="interactions" value="1311"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DI71"/>
    <dbReference type="GeneID" id="8292083"/>
    <dbReference type="KEGG" id="lth:KLTH0E10186g"/>
    <dbReference type="eggNOG" id="KOG1744">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075666_1_3_1"/>
    <dbReference type="InParanoid" id="C5DI71"/>
    <dbReference type="OMA" id="AQLCQTT"/>
    <dbReference type="OrthoDB" id="10254238at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22910">
        <property type="entry name" value="HFD_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000014">
        <property type="entry name" value="Histone H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000558">
        <property type="entry name" value="Histone_H2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055333">
        <property type="entry name" value="HISTONE_H2B_site"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23428">
        <property type="entry name" value="HISTONE H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00621">
        <property type="entry name" value="HISTONEH2B"/>
    </dbReference>
    <dbReference type="SMART" id="SM00427">
        <property type="entry name" value="H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00357">
        <property type="entry name" value="HISTONE_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5 12" id="KW-0158">Chromosome</keyword>
    <keyword evidence="9 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="11 12" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="10 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="14">
        <location>
            <begin position="25"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR23482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="132" mass="14349" checksum="4ADD0037DE4750A7" modified="2009-07-28" version="1">MSAKAEKKPASKAPAEKKPAAKKTASSVDASKKRTKVRKETYSSYIYKVLKQTHPDTGISQKSMSILNSFVNDIFERIASEASKLAAYNKKSTISAREIQTAVRLILPGELAKHAVSEGTRAVTKYSSSTQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIJ9</accession>
    <name>C5DIJ9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0E13112p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0E13112g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the alkaline ceramidase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554047.1">
        <property type="nucleotide sequence ID" value="XM_002554001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIJ9"/>
    <dbReference type="FunCoup" id="C5DIJ9">
        <property type="interactions" value="577"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIJ9"/>
    <dbReference type="GeneID" id="8292216"/>
    <dbReference type="KEGG" id="lth:KLTH0E13112g"/>
    <dbReference type="eggNOG" id="KOG2329">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063293_3_0_1"/>
    <dbReference type="InParanoid" id="C5DIJ9"/>
    <dbReference type="OMA" id="IMFEPLR"/>
    <dbReference type="OrthoDB" id="187171at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046514">
        <property type="term" value="P:ceramide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008901">
        <property type="entry name" value="ACER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46187">
        <property type="entry name" value="ALKALINE CERAMIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46187:SF3">
        <property type="entry name" value="ALKALINE CERAMIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05875">
        <property type="entry name" value="Ceramidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="69"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="180"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="210"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="248"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR608901-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR608901-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR23610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="335" mass="37841" checksum="B071139EDF134D20" modified="2009-07-28" version="1">MLSSIFKAYPEPPEVGYWGKPTSTIDWCEENYVVSPFVAEWANTVTNGLFVLLAVFVTWSAVHNRLEKRFAMIGLGLGTVGVGSWLFHMTLKYEYQLLDELPMIYATCIPAWSIFCEERQLLAKGADGANGTAGTVAVARKPTLLKQAVVALLVFGTATALTVIYLVYRDPTIHQTAYAVLNVLVVFSAGALASKYVSDPRARRNLRDTMALSISIFLTGYLLWQLDVHFCSFWIFIRRSLLKLPLGILLELHSWWHLLTGTGVYFYIVHLEYLRVLTHDQADQYTLVWHWKVFPELVLNAHRGSTKYSLEFMDKYVSDSGPGSAGPKDDRRKSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIU8</accession>
    <name>C5DIU8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0E15334p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0E15334g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GID4/VID24 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554146.1">
        <property type="nucleotide sequence ID" value="XM_002554100.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIU8"/>
    <dbReference type="FunCoup" id="C5DIU8">
        <property type="interactions" value="337"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIU8"/>
    <dbReference type="GeneID" id="8292317"/>
    <dbReference type="KEGG" id="lth:KLTH0E15334g"/>
    <dbReference type="eggNOG" id="KOG4635">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028759_3_0_1"/>
    <dbReference type="InParanoid" id="C5DIU8"/>
    <dbReference type="OMA" id="NILGFYY"/>
    <dbReference type="OrthoDB" id="62at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034657">
        <property type="term" value="C:GID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045721">
        <property type="term" value="P:negative regulation of gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007039">
        <property type="term" value="P:protein catabolic process in the vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018618">
        <property type="entry name" value="GID4/10-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14534:SF3">
        <property type="entry name" value="GID COMPLEX SUBUNIT 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14534">
        <property type="entry name" value="VACUOLAR IMPORT AND DEGRADATION PROTEIN 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09783">
        <property type="entry name" value="Vac_ImportDeg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00061469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="323" mass="35722" checksum="C4398D7E14C8DFA6" modified="2009-07-28" version="1">MINETVKPLKQVLASPIASRSASPPPALPHRLVQSCKDEDTLYRCTSVDSLDSLQHSFRSPSTSADGSRKYISEDESSYGDVAAVAHSPRASPAAWTCCTRQLRPRTRFTGFQISDYKKYQVSVTLKTVDLPASSFCTASTPHVTGFLSICGLTAQHPEITTFFEGYAVTDNIGFLSSAMPSELDALKANDRTDLDHWLSFPCFKELCSPQDSAAEDAAKPAASAEDSKPDTLGAIVQGSYSHTDFMQNRFIYMRWKEKFLVPDADVDSVEGASYDGYYYIVHDQLTGHIVGFYYHKDAEKFQQLELTPVTDECSGSCDFEFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJ27</accession>
    <name>C5DJ27_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1</fullName>
            <shortName evidence="8">Oligosaccharyl transferase subunit WBP1</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0F12958g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER).</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the oligosaccharyltransferase (OST) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endoplasmic reticulum membrane</location>
            <topology evidence="8">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the DDOST 48 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554753.1">
        <property type="nucleotide sequence ID" value="XM_002554707.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJ27"/>
    <dbReference type="FunCoup" id="C5DJ27">
        <property type="interactions" value="1094"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJ27"/>
    <dbReference type="GeneID" id="8292976"/>
    <dbReference type="KEGG" id="lth:KLTH0F12958g"/>
    <dbReference type="eggNOG" id="KOG2754">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031804_1_1_1"/>
    <dbReference type="InParanoid" id="C5DJ27"/>
    <dbReference type="OMA" id="AHDEYPR"/>
    <dbReference type="OrthoDB" id="29105at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008250">
        <property type="term" value="C:oligosaccharyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018279">
        <property type="term" value="P:protein N-linked glycosylation via asparagine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005013">
        <property type="entry name" value="DDOST_48_kDa_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055459">
        <property type="entry name" value="OST48_MD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055457">
        <property type="entry name" value="OST48_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10830">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE 48 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10830:SF0">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE 48 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23358">
        <property type="entry name" value="OST48_MD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03345">
        <property type="entry name" value="OST48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="403"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="OST48 N-terminal" evidence="9">
        <location>
            <begin position="36"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="OST48 middle" evidence="10">
        <location>
            <begin position="282"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR24316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="439" mass="49999" checksum="92F38EF6D4A1DFAF" modified="2009-07-28" version="1">MRVVGVFRNVVRTLFMAIAVMPILTLVEAKPAFGSRTLVIYDQRLTELDSYSTFFNSLKERSHELTYRALGNDSTPISLFRGEDRLYDNLIVYPVRSRHFNKQLSVENLLDFFSSGGQVLTVTSPEGLSDNIRSFLNQLGIYPSPRNYYVNDYYHGDENNTLYLTKGKSLNDYVVSPGDYNLVYKGAAALLDNRQLIIPVLQAPKTSFTLDSKQSNEEWTIGSEGYLAAAFQSLHNTRLAWVGSEVFLSDEFSASNGRFAEELTKWTFGEKAVLKSVGFTHHHASGASYLESPYKVKDSIVYEIGFSEWNGSSWGPFTSDDVQFELRMIDPYYRITLLPSETSSDAQYYTTGNFSLPDHHGIFTFLVDYKRAGLSFITEKDVRAIRHLANDEYPRSWDITNSWVYLTSIYSVITVWVLFVVLYLSLRKPKTVETGKKNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJF2</accession>
    <name>C5DJF2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0F15928p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0F15928g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554878.1">
        <property type="nucleotide sequence ID" value="XM_002554832.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJF2"/>
    <dbReference type="FunCoup" id="C5DJF2">
        <property type="interactions" value="177"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJF2"/>
    <dbReference type="GeneID" id="8293109"/>
    <dbReference type="KEGG" id="lth:KLTH0F15928g"/>
    <dbReference type="eggNOG" id="KOG2428">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052846_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJF2"/>
    <dbReference type="OMA" id="TNIYRWS"/>
    <dbReference type="OrthoDB" id="20844at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016593">
        <property type="term" value="C:Cdc73/Paf1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990269">
        <property type="term" value="F:RNA polymerase II C-terminal domain phosphoserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007149">
        <property type="entry name" value="Leo1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23146">
        <property type="entry name" value="LEO1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23146:SF0">
        <property type="entry name" value="RNA POLYMERASE-ASSOCIATED PROTEIN LEO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04004">
        <property type="entry name" value="Leo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="240"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="256"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="273"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="298"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="311"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="331"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="354"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="388"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="423" mass="48347" checksum="09726250D2970B95" modified="2009-07-28" version="1">MSDQGEGTVALENSQNELSEKQSPNVEADGEEMDDLFGDDSDQETGSGPGEQEPHSPNSANEEEDDEQAMYNRKFYGDKGEDSNDEGSQEFREADIDIMKHIVPYSTEPSQGDKTIYYTKVPQFLTIDPIPFDPPAFQSKIEERAAQRTSVEDQIDDRLIEENTVRWRYSRDKNQRVFKESNAHIVQWSDGTYSLRLGDEYTDVLINDTEDTYLAVSHDQQELIQSVKGGEVTKNMIFIPTSTNSKSHQRLSKAVARKEQQEHHGPNTYILRVDPELEQKELEKKQGQIIRERRKRQLREQQEREGMDSPAPTSKPRSASRPGYSQTRYDEYEDDGFMVDDEENESDGGVGGDGNEDEEDLLDDEEEEDDDDLNAERLRQLKRKGAAQYNTQEASDNNVESDDAEDSAMKRRKVAVLDDEDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJG3</accession>
    <name>C5DJG3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Pre-mRNA-processing factor 17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0F16192g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554889.1">
        <property type="nucleotide sequence ID" value="XM_002554843.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJG3"/>
    <dbReference type="FunCoup" id="C5DJG3">
        <property type="interactions" value="928"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJG3"/>
    <dbReference type="GeneID" id="8293121"/>
    <dbReference type="KEGG" id="lth:KLTH0F16192g"/>
    <dbReference type="eggNOG" id="KOG0282">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022571_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJG3"/>
    <dbReference type="OMA" id="VQVYDHH"/>
    <dbReference type="OrthoDB" id="10257301at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000034">
        <property type="entry name" value="Pre-mRNA-processing factor 17, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032847">
        <property type="entry name" value="PRPF17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43979">
        <property type="entry name" value="PRE-MRNA-PROCESSING FACTOR 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43979:SF1">
        <property type="entry name" value="PRE-MRNA-PROCESSING FACTOR 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="2 9" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="165"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="209"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="296"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="398"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="13"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="34"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="53"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00068146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR24452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="462" mass="52451" checksum="A591A789556FDCCF" modified="2009-07-28" version="1">MPLVEGYSSDSDSSSHSHAESNKLPKAVKDQENNDSISVDLTQLEPSNQVAKRPNHNTTRAKRKRRKGKGPWASWSSSEESEEEPGPEVASEAEKESGILLEDEEFQLSRDEKTLSSESTRFYGDSVTDYQGRSFLHPPIEVDIDFTKPELSFRCYLPKKMIHSYRGHHNGTTTIKMLPKSGHLFLSGGNDNKVKLWDVYHKRELLRDYCGHSKAVRDVSFSGSGTSFLSVSYDQHMKIWNTETGDIEHRYKFPAVPNCAEFSPANSNELIVGLSNSEVRHYDLRVAHKDGLVQVYDHHLSSIIALKYFPDGSKFISSSEDKSMRIWDNQVNIPIKQISDTAQYSMPFIDIHPEHHYFATQSMDNAIYAFSMKPKYRRNPKKRFEGHKCAGYGIGFGFSPDGQYLASGDTKGRVYIWDWKTTRLLKHFEVPGKKAVITVAWAPQETSKMLCAGNGGRIFLYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJN9</accession>
    <name>C5DJN9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0F17952p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0F17952g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554965.1">
        <property type="nucleotide sequence ID" value="XM_002554919.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJN9"/>
    <dbReference type="FunCoup" id="C5DJN9">
        <property type="interactions" value="186"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJN9"/>
    <dbReference type="GeneID" id="8293200"/>
    <dbReference type="KEGG" id="lth:KLTH0F17952g"/>
    <dbReference type="eggNOG" id="ENOG502S0A4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089054_1_0_1"/>
    <dbReference type="InParanoid" id="C5DJN9"/>
    <dbReference type="OMA" id="PEKYTRK"/>
    <dbReference type="OrthoDB" id="6021263at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024388">
        <property type="entry name" value="Ribosomal_mL58"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28266">
        <property type="entry name" value="54S RIBOSOMAL PROTEIN L20, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28266:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12824">
        <property type="entry name" value="MRP-L20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR24528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="199" mass="22908" checksum="2E9C0613DCE87AE0" modified="2009-07-28" version="1">MSSIRQLHVSALLRQDVQNTSKNVKKISQLKSVPNLYNSKSSASNYKGALRAKIQPGVYLQTSQASSTGSINSETIPKAFLPKDDPRRRFVQMLRPRENLQSTWAPALHQKKEKSYHLTPEQVAEIQRLRSGNPEKYTRKVLAQQFGVSPFFISLVSSAPESRVAEMAERLEHIKQSWHPKRVLAREDRKKRKELWYRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJQ9</accession>
    <name>C5DJQ9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">KLTH0F18392p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0F18392g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554985.1">
        <property type="nucleotide sequence ID" value="XM_002554939.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJQ9"/>
    <dbReference type="FunCoup" id="C5DJQ9">
        <property type="interactions" value="535"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJQ9"/>
    <dbReference type="GeneID" id="8293220"/>
    <dbReference type="KEGG" id="lth:KLTH0F18392g"/>
    <dbReference type="eggNOG" id="ENOG502QPVP">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015609_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJQ9"/>
    <dbReference type="OMA" id="MQIGLHQ"/>
    <dbReference type="OrthoDB" id="2341546at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001216">
        <property type="term" value="F:DNA-binding transcription activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.240.10:FF:000003">
        <property type="entry name" value="C6 transcription factor (Leu3)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051089">
        <property type="entry name" value="prtT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31845">
        <property type="entry name" value="FINGER DOMAIN PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31845:SF21">
        <property type="entry name" value="REGULATORY PROTEIN LEU3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="10">
        <location>
            <begin position="30"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="131"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="672"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="76"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="135"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="688"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="724"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="761"/>
            <end position="787"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR24548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="873" mass="97805" checksum="0F95E862392E1C60" modified="2009-07-28" version="1">MSESSDFQDEKTVSSDINAGLRKGRRKKLACVECRQQKSKCDAHERAPESCTRCAKKNVQCVLQKDFRRTYKRVRNEVIEKRFKELTKSLSNLGAEEILRRIEEEQQTLLDQNNFTKEKLMKLRQDAASGLDKASSTLSTSSVDDTPTAEPPIESLSAEDRNRESEEAAVTIEVPTSANVFNSNIIHCSPKSLGDVYMSSEDIRDLYEEFTAKYHPFLPVVDISKGPELIYSLSPCLFWVIMLIGLRRKSKSSDIMAKLSTLVKSVLAEITISPIIRYAPSENDEPVLNVASVYSVQAFLLYTFWPPLTSSLSADTSWNTMGSAMFQAIRVGLNCAEFSTEYATTNSEQIQEQVKTWVCCNIVSQTIASSFGFPSFVSFDHTVISACELSSRQGGSYQDTYVPRSIKQMMHIAYFENQLTKTMNSNPLNAMGMVGGGEKFPLLQVLEQQLGELELRLRDSDLDDIRKFLLLVSKVHLLTYCFTGSKPSDSSAGFDAIDATVRDIETNFEVKRGFVKVYNAAIELLLHTSAMWKSDPAVIKSFPGVFVLNIWQTACIISKLVHSSLDSVLNLKLGQKVYQDAVLLTFNASVLKYDMAYRSSGIMRSIWSMFSNMHEKWKNKQAGQERRVEGDFNLDITIKSRMSASVFFDCLYILRSKCGMAKLKREMRRHSIDNDGEEELQKGPNDVGHPKGKQERRLSGNKHPEENARKLIKTIPLDPVPINAPASTGSSSASPGSQAGDVPSLRSILNRRLPRPDEARTQNSGSTFNSPQSGAGGQHTATSTEGQRANHLDLAPDALIEQSLLDPRDTSEHTSLFHRPMPVENNESIDQPILSSHRSDSPVTMEHWDNWESDMVWKDVDILMNEFAFNPTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJU3</accession>
    <name>C5DJU3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0F19162p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0F19162g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555019.1">
        <property type="nucleotide sequence ID" value="XM_002554973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJU3"/>
    <dbReference type="FunCoup" id="C5DJU3">
        <property type="interactions" value="623"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJU3"/>
    <dbReference type="GeneID" id="8293255"/>
    <dbReference type="KEGG" id="lth:KLTH0F19162g"/>
    <dbReference type="eggNOG" id="ENOG502S89R">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084829_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJU3"/>
    <dbReference type="OMA" id="NMELEIC"/>
    <dbReference type="OrthoDB" id="4066471at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="182"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="289" mass="33320" checksum="E195FFEA30454FA5" modified="2009-07-28" version="1">MSNPDDLLLELIYEYKPHLQSYRHRMNTWNELLRAFNRKTGASYRQNRTLKTRFEKLKELYINNEELPFSNVGLLEELLKEDRRDYKYAKIRQPPSSAENGDSEPPPSSDTIPQLIHQKDERSDPDIRSNEEAIGTQPPPLDSITVFPHTQMRRYQEHQDQHRDFSPGSHLHPQLGSSSVHNSNTPSDTSTSSSRFSSNKQQALSMVDSLRNDMGGERTYADSLELQNCTTIDALQKEVALLKQNQQRFQKDVMLKLNKIAELLQEREPENQAGFLNLPFSTNTQHSPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJY2</accession>
    <name>C5DJY2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0F00242p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0F00242g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554220.1">
        <property type="nucleotide sequence ID" value="XM_002554174.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJY2"/>
    <dbReference type="STRING" id="559295.C5DJY2"/>
    <dbReference type="GeneID" id="8292409"/>
    <dbReference type="KEGG" id="lth:KLTH0F00242g"/>
    <dbReference type="HOGENOM" id="CLU_1816149_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJY2"/>
    <dbReference type="OrthoDB" id="2109411at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0371">Homeobox</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="2">
        <location>
            <begin position="59"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR23783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="142" mass="16470" checksum="B4C2DDC8440D03F1" modified="2009-07-28" version="1">MDSRGLTISLESIKKVCLMLLEPNYKSRQEVCDKIYENYATLLQDAVVESKEKPRSGQQRRTLLSKDTRLFLESVFEKKRSPNSRERKAIADKCGLTPIYISFSVFSSSQGHGSQQKTFPTYSPIFLTFFMFWSNFRTFNTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DK15</accession>
    <name>C5DK15_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0F01012p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0F01012g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>beta-nicotinamide D-ribonucleotide + ATP + H(+) = diphosphate + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:21360"/>
            <dbReference type="ChEBI" id="CHEBI:14649"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="EC" id="2.7.7.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; NAD(+) biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554253.1">
        <property type="nucleotide sequence ID" value="XM_002554207.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DK15"/>
    <dbReference type="FunCoup" id="C5DK15">
        <property type="interactions" value="55"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DK15"/>
    <dbReference type="GeneID" id="8292444"/>
    <dbReference type="KEGG" id="lth:KLTH0F01012g"/>
    <dbReference type="eggNOG" id="ENOG502RXY8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032651_0_0_1"/>
    <dbReference type="InParanoid" id="C5DK15"/>
    <dbReference type="OMA" id="RLVMMEL"/>
    <dbReference type="OrthoDB" id="5591297at2759"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00600"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000309">
        <property type="term" value="F:nicotinamide-nucleotide adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd02165">
        <property type="entry name" value="NMNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005248">
        <property type="entry name" value="NadD/NMNAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31285">
        <property type="entry name" value="NICOTINAMIDE MONONUCLEOTIDE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31285:SF0">
        <property type="entry name" value="NICOTINAMIDE MONONUCLEOTIDE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="2" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="9">
        <location>
            <begin position="44"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR23816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="263" mass="30041" checksum="1D26838F471A699A" modified="2009-07-28" version="1">MLYRSPEGGKCLQILKRFQESISAFEIFHGQRHLDKCRRVLVLDSSFNPPHMGHYTLVERAVKYYGSTDLQVILLLSINNADKEIKPASLDKRMDMMCIMADLLSKNSLPVSVGITKYAKFFEKSTAISKELGHSPKISYLVGFDTIVRVFDSKYYAPLSVADALRDFMSETEFFCLTRDGETAVQQQLQYPGDIAKGVYEPNIPKSWHSKVVVEKGNEFFSHVSSSSLRKTLYDPNKDVSASVPPEIYHYIKNQFPYDIFKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DK59</accession>
    <name>C5DK59_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0F02002p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0F02002g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554297.1">
        <property type="nucleotide sequence ID" value="XM_002554251.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DK59"/>
    <dbReference type="FunCoup" id="C5DK59">
        <property type="interactions" value="227"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DK59"/>
    <dbReference type="GeneID" id="8292489"/>
    <dbReference type="KEGG" id="lth:KLTH0F02002g"/>
    <dbReference type="eggNOG" id="ENOG502QPV9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006977_1_0_1"/>
    <dbReference type="InParanoid" id="C5DK59"/>
    <dbReference type="OMA" id="TLPHIYY"/>
    <dbReference type="OrthoDB" id="5563754at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="CDD" id="cd00821">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058155">
        <property type="entry name" value="Skg3/CAF120-like_PH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25381">
        <property type="entry name" value="PH_26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="75"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="488"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="592"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="630"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="792"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="25"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="488"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="501"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="519"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="630"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="643"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="801"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="836"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="890"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="973"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1016"/>
            <end position="1032"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR23860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1032" mass="113472" checksum="64B9B45357E4132C" modified="2009-07-28" version="1">MRSPTKGLLAPPKTFQKSDDKNSRSRSSSFSRLFSSGSSDNSPRRQVPPLQEISTSAIPPELVPIVTLLSAQSHRRYHEGVFLILKDLNSDGSPATRTWKEVYGVLIGNQLALWDARLLAENGQRPEDLMEATSKPTYVNFTDASFRPLDHKEAVIAEGKKQLQNTIVVSTTLKNRYFLQFSDKEAFTQWHAALRLSAFEFTALQEAYTGAFLSTKGAKLGDIRVILADTKFDYEDWVSVRFGAGMPWKRCYAVVSQPSKKSKSKLICGEINFYENEKKTKKVKAMTTVKDACAVYALYPSSPVLIDTSTMIKLEGKVSFGKKDAPTLTDIFIMPEKHYAVPGYDTIIRFLIPIMNAFQLYGRPKRLIADRNNLDSLLFGLPTLPHVHFLQLQDIMPLASSASNMEWSILDWRNHIKALLLKKIDQGYTGCGSSEGLTGALASPAIGSNDLFDSSPLPTSPLLASVSRLPSENKTEFGSIKSNLRNAATTDNIDGNTSKSGLHDSSSKASDDKGFVTETAPSATQVSRNGGSFEDVSDDKKQSRKKLTPTIDIQDSNFSEFEREALKPKTSEALGFRKSELSTIYDKYSQMPFGDSAGQPPSLPIDRDNRDSFGAYNDYIGSPKVKNLDISNLRDSNSTDQTDLSRVHDFSGEGGRRSFADSFQSGEPAPAEERSVKDDVLEDFYSLSQQISKMGLDSQDRASFSASKANVNDDFNFEASETNDSIGALDNVFDPDYLEQNQMLDNESNYTANESKSQYSGDNTNLSYQPLQRDQAQPSYGGEGFTYSQKLPKQRAANHVVTPTSPQFYPNHPVENQNAGSAERLGKMKHSPAYPNLNTVNTQTNGSPSHRRNPVPPPPQGAQYSPNNMQHRRPGPQGVPSGAPRPVPQQYPQQVPQQAFQQVPRAMSKPVPQQVPYSNRPYVPAGAPNAYGRPNPPAQYPPTGAVPLGQPGNPRGPGYAMGPHPQARPMRHTASQTMNQAAPQVFSQSIPHPNSKLQAKSAQPSPRAGGFSQFMPATANNPNPYSANPYTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>C5DK76</accession>
    <name>C5DK76_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0F02376p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0F02376g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the formin homology family. BNI1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554314.1">
        <property type="nucleotide sequence ID" value="XM_002554268.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DK76">
        <property type="interactions" value="553"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DK76"/>
    <dbReference type="GeneID" id="8292506"/>
    <dbReference type="KEGG" id="lth:KLTH0F02376g"/>
    <dbReference type="eggNOG" id="KOG1922">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001313_1_0_1"/>
    <dbReference type="InParanoid" id="C5DK76"/>
    <dbReference type="OMA" id="NHYWKAR"/>
    <dbReference type="OrthoDB" id="1104827at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005934">
        <property type="term" value="C:cellular bud tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000131">
        <property type="term" value="C:incipient cellular bud site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005522">
        <property type="term" value="F:profilin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045010">
        <property type="term" value="P:actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051016">
        <property type="term" value="P:barbed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071474">
        <property type="term" value="P:cellular hyperosmotic response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030010">
        <property type="term" value="P:establishment of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070649">
        <property type="term" value="P:formin-nucleated actin cable assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903475">
        <property type="term" value="P:mitotic actomyosin contractile ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.2220:FF:000006">
        <property type="entry name" value="Cytokinesis protein sepA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.30.50:FF:000001">
        <property type="entry name" value="Cytokinesis sepA protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000898">
        <property type="entry name" value="Formin BNI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.30.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2220">
        <property type="entry name" value="Formin, FH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.150">
        <property type="entry name" value="Formin, FH3 diaphanous domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051661">
        <property type="entry name" value="Actin_filament_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014767">
        <property type="entry name" value="DAD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015425">
        <property type="entry name" value="FH2_Formin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042201">
        <property type="entry name" value="FH2_Formin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010472">
        <property type="entry name" value="FH3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014768">
        <property type="entry name" value="GBD/FH3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010473">
        <property type="entry name" value="GTPase-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47102">
        <property type="entry name" value="PROTEIN BNI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47102:SF2">
        <property type="entry name" value="PROTEIN BNI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06367">
        <property type="entry name" value="Drf_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06371">
        <property type="entry name" value="Drf_GBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02181">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01139">
        <property type="entry name" value="Drf_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01140">
        <property type="entry name" value="Drf_GBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00498">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51231">
        <property type="entry name" value="DAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51444">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51232">
        <property type="entry name" value="GBD_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GBD/FH3" evidence="6">
        <location>
            <begin position="167"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="domain" description="FH2" evidence="7">
        <location>
            <begin position="1332"/>
            <end position="1750"/>
        </location>
    </feature>
    <feature type="domain" description="DAD" evidence="5">
        <location>
            <begin position="1779"/>
            <end position="1809"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="115"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="227"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="463"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="789"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="866"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1005"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1136"/>
            <end position="1337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1751"/>
            <end position="1779"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1791"/>
            <end position="1814"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1836"/>
            <end position="1976"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="703"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="899"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="9"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="32"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="229"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="260"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="793"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="872"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1151"/>
            <end position="1165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="1213"/>
            <end position="1222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="1249"/>
            <end position="1261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1262"/>
            <end position="1275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="1276"/>
            <end position="1289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="1298"/>
            <end position="1309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1313"/>
            <end position="1330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1751"/>
            <end position="1764"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1804"/>
            <end position="1814"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1842"/>
            <end position="1859"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1867"/>
            <end position="1881"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1957"/>
            <end position="1976"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00037935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR23877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1976" mass="218910" checksum="B8B71221DD2DB3FA" modified="2009-07-28" version="1">MNRSSNSKSSKAPHNRSASSSSGNGGIFSNLKRLTTGSGNSSVSHNTQKLDISDISSPKKINLPDTGEFSHKPLSKQSTLNMASLSAYTDAVGAHNRSASNASVGSPTKYSYSRRASQWSNNNSAVSGSKLSRQQTNQSMSSASIFSQGSFSNLSKFVGPDGAVRLERPRDPKEIEELFEEVLYKRNVYQSLPASAQRELNNYDLEKKWLMVRQDLQSEVKKFMNSKNASKSSTVVGGSSSAVPDSPSASNFTSSTSPNGSFTGLSRPKISTSSVNTSEQFYSSNLGTSSTTTLSQDPSHLSPDYYVRKIICNDISAKRLNDLWVSLRTEQLDWVQGFLEAQGQVAIANVILKTCYRESPDNLLGDEVLDKEFAYFKCLKTSLNLREGADEAVMSNSARIIVSAIVEGLLSLRVATRRVASELLISLSQWSLPHGFNHVMDALDQESRFCDNVHLQARLITQSASKDPKKGSSIAPTPDGDGDRVMRKFEQWMLVVEYTLDGRGKMGSLVGASEDFRTSGGENAIMEYAYLTLLLINHLCQTPVDVKQRTVLRARLKNAGLPRILNKMKLLNYEKVDEQLARFDDSTTDDFDALYSQESTGEGVDMKDPVSMTQNLWNLCKGTDAEQHLTSLLQNLLISTGELGSKNKDDPTQRTKQLKLIDALVSNVSMASVDMQSSFNSAIQRLYDAMQTDEIARRAILENRDWVKRYEEIKADRDNLKEKLSNAEGGLVGQLQDEVRQRDHILEKSQRVNAQLQHELDESKKKLILAKHEHEVELRKTLTAMNSNFEGSHNMRDEKGSENPRPLRPERKLAIQKALQAKLEKTSKEINVESKRLGLSLEPNKRLKLLRSRMEDIENQARELEMTNFSDYQKDDIKEPEVSNEDDHKAVSESEEKAQQISALKLEELRKKLASLQQESNDISKFNVEGRFHEMFSGQKSLALDRLKKLETDYKGFGINFDPDSPLGQALAGKSVSSDDKARTLDPKEALNIVEEVSNILSGLDSSKAAEKSNETPLRTATSSDSSEDEMDDKNGDKIAADSTLPTSSFLESLSQKYGGAQNSLSNRHSFAGGEINYPGSGYHRKSFMNRVKRTGAVPYLGELSGKIGSSSYIKPSEAEENVGIGIMVDEKPLSRRVQEDAAGVSQLESNEEKPALSHDLDKNTTHSKPITIGENLAAAKTAGKQQAHEDEGTTEGSSTDKDSHEIASATSAPPPPPPPPMDLFAKNGAPVKTDNPDPAVSDTKSAAAPPPPPPPPPIPPMLASSSSGSTSKAGAPPPPPPPPPPPPALLSQSSNNGPPPPPPPPPPFGASVSRNKSATPSPMLPQSPSLFDRYPRPSKKLKQLHWEKIDDAEDSIWRDAKAEKFADDLYEKGVLSRLEKAFAAREIKSLASRKKKDSDKLSFLSRDVSQQFGINLHMYSSLSVEEVVSKILRCDRDFLSTPSVIDFLSKQEIVEVSNNLARNFAPYTIDWEGVSSVENAKPPEKDPTELQRADRLYLELFVNLQTYWSSRMRALKVITTYEKDYSDLVHKLSMIDKATCSIQQSENLRNVLDVILAVGNFMNDSSKQAQGFRLATLQRLTFIKDDKNSMTFLNYVEKIIRETYPEFNDFLKELEPVVAAVKISIEQVAQDCREFSQSVINVERSVDIGNLSDPTKFHPSDRVLLKVLPTLPEARKKGDLLMDEMKLTLLEFDNLMRLFGEDAMDKFARNSFFKKFADFLLEYKKAQVYNLKLEEEERAYERRKKLVEDQLKRTRESESKNKGTTDAGGDSVRNEVNEDRDVMDRLLEKLKNAGPAKSDPSSARKRAVARKRLLQGSSSNSTILDNFDIEDSDGKSLVYSPDNNSSEVQLNEADTSPTPERRVRDSNATSPASHDTGSSDLTDRARNLLIELRGPESPGVKMSAQHQRLSKLRARRKNDSSSSGSENRLNFVGAGQAENSDEQNEPEVLKSKPESANDDDNGEQSDSSDGEFLDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKM6</accession>
    <name>C5DKM6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0F05940p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0F05940g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554464.1">
        <property type="nucleotide sequence ID" value="XM_002554418.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKM6"/>
    <dbReference type="GeneID" id="8292662"/>
    <dbReference type="KEGG" id="lth:KLTH0F05940g"/>
    <dbReference type="eggNOG" id="ENOG502QSXK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008372_1_0_1"/>
    <dbReference type="InParanoid" id="C5DKM6"/>
    <dbReference type="OMA" id="LWRPCVG"/>
    <dbReference type="OrthoDB" id="1612078at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021102">
        <property type="entry name" value="PNGase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056948">
        <property type="entry name" value="PNGaseA_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31104">
        <property type="entry name" value="PEPTIDE-N4-(N-ACETYL-BETA-GLUCOSAMINYL)ASPARAGINE AMIDASE A PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12222">
        <property type="entry name" value="PNGaseA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptide N-acetyl-beta-D-glucosaminyl asparaginase amidase A N-terminal" evidence="2">
        <location>
            <begin position="110"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR24027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="651" mass="73639" checksum="024F5AB4DA0871A5" modified="2009-07-28" version="1">MGRKGTDMATEKELERPGVQDLQGNAASDPNFDYEKQGLARKNKGGSRPWKHRRNIAVIVCLLLVNLFLRFDKNTMFGAKVHGDLSSPQNRVQHSFEVTVEKLPSTDPVFTQHLLSSSFGDSWGKPAKAHFEPYNGGKYDKIILELVTNVTGRQFDRLLHVFIDDINVWRSSTVEPWGNRTIVSEAIKDISQYRALFDRESLEITLQLDNLVTHRLTGVFNVELRAHYYQTSNDTDVSKPSLREKLLQYFTSPANLITPLVSHFSRTPLFYYPRASQGNPRWSRSLPELEESSNISRAMIEIFASGNAAEEFWYTNVLDRFTNRFRKSGHELLGHGPFRAIKIYLTNSEKEILVDSVVPTPIIFTGFIPPLWRPCVGMNAFDLESYKVDLTPFLSLIEGESWELQLEVVSSASSNFKPTVGENWIISGNVFVWNNTLDATQPSFTNSSMLPSSFDVFVNDSKPDELIQNVSAKNGVLVESTITLDGVEYQLHQTVESTFTSNQKYLENGDVEKTFVDLSKERKWVFRKADKDLFEFTDRTGWDFVGSVKTFTSEKDSSQLTYAADVARSLERHITLQEKDSVGHQVGNDNVLLSLAGSQVGQANYTLSSNGNYGSGNSVHSVCVERAWPYEDNYERTVVVADNEVVLDDYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKN0</accession>
    <name>C5DKN0_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">U6 snRNA-associated Sm-like protein LSm1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LSM1</name>
        <name evidence="8" type="ordered locus">KLTH0F06028g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the cytoplasmic LSM1-LSM7 complex which is involved in mRNA degradation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the heptameric LSM1-LSM7 complex that forms a seven-membered ring structure with a donut shape.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
            <location evidence="6">P-body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554468.1">
        <property type="nucleotide sequence ID" value="XM_002554422.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKN0"/>
    <dbReference type="FunCoup" id="C5DKN0">
        <property type="interactions" value="514"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKN0"/>
    <dbReference type="GeneID" id="8292666"/>
    <dbReference type="KEGG" id="lth:KLTH0F06028g"/>
    <dbReference type="eggNOG" id="KOG1782">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076902_0_1_1"/>
    <dbReference type="InParanoid" id="C5DKN0"/>
    <dbReference type="OMA" id="FMVRGEN"/>
    <dbReference type="OrthoDB" id="10263346at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990726">
        <property type="term" value="C:Lsm1-7-Pat1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01728">
        <property type="entry name" value="LSm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000045">
        <property type="entry name" value="U6 snRNA-associated Sm-like protein LSm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034104">
        <property type="entry name" value="Lsm1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044642">
        <property type="entry name" value="PTHR15588"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15588">
        <property type="entry name" value="LSM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15588:SF8">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Sm" evidence="7">
        <location>
            <begin position="29"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR24031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="160" mass="18277" checksum="5B2275D7918D81E3" modified="2009-07-28" version="1">MSEMKSAPSGASKISEGEADLYLDQYNFTTTAAIVGSVDRKIFVLLRDGRMLFGILRTFDQYANLILQHCVERIYITEENKYAECGRGVFMVRGENVVMLGEVDIDREDQPLSQMERISFEEASAVKKQRDDARCELESKKGKTMAQHGLIYEFHKSDIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKN3</accession>
    <name>C5DKN3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0F06094p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0F06094g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554471.1">
        <property type="nucleotide sequence ID" value="XM_002554425.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKN3"/>
    <dbReference type="FunCoup" id="C5DKN3">
        <property type="interactions" value="265"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKN3"/>
    <dbReference type="GeneID" id="8292669"/>
    <dbReference type="KEGG" id="lth:KLTH0F06094g"/>
    <dbReference type="eggNOG" id="ENOG502S14D">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103824_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKN3"/>
    <dbReference type="OMA" id="HHKVHSL"/>
    <dbReference type="OrthoDB" id="4086742at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022784">
        <property type="entry name" value="Ribosome_bgen_Alb1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09135">
        <property type="entry name" value="Alb1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="61"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR24034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="161" mass="17645" checksum="16BDE19DDFDCCFF8" modified="2009-07-28" version="1">MPSKNSINRPKQQMNLNRKVHSLAKKRDARERAGLLQPARSHETSKSGQPKSVPIDLYKGEQSSAGPVTTKTLSKKRAKKIERNMRYAEQRKLLVDVQKKAEDGMEVDGEVAASIAGSDAAKKSQFARVKDAFFRAMEDTASSGLVLENGAGTTLGGRFFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKP2</accession>
    <name>C5DKP2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0F06336p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0F06336g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554480.1">
        <property type="nucleotide sequence ID" value="XM_002554434.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKP2"/>
    <dbReference type="FunCoup" id="C5DKP2">
        <property type="interactions" value="55"/>
    </dbReference>
    <dbReference type="GeneID" id="8292680"/>
    <dbReference type="KEGG" id="lth:KLTH0F06336g"/>
    <dbReference type="eggNOG" id="ENOG502S61D">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_829162_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKP2"/>
    <dbReference type="OrthoDB" id="4065415at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR24043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="335" mass="37800" checksum="5E80F93C0959B8BD" modified="2009-07-28" version="1">MLDHFPTELQLRLLACNPQLAFVSRGWYRLNNLFYRDLCVQLRPESYWKGLATWICAYVKSLDPDRKDARLINRGFMRDTGGEFVEFMCDSWQILYSVLFASPRFFGMDSARMEIEDSSNSVSEYCCPMELVGGREYECSVWFRKRSTLNKFGAVRVVCEADAPREAGAAGLVGGAGAVLLARDLPVSIDNWCESAGMYSFAAGKIRVPPELGHVQDARMRVWITSPTSVEMVAVDLRPYQRGKQWLFLSTPQQGIFSAEEQRLSKARMGWAMERTGNEEAPAAGAGAGAPAEDFRVVYRFPREQGKALKVRSVRYPWLGQGAVTRTLDRVRNIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKQ0</accession>
    <name>C5DKQ0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">KLTH0F06534p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0F06534g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <location evidence="3">Coated pit</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the adaptor complexes medium subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554488.1">
        <property type="nucleotide sequence ID" value="XM_002554442.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKQ0"/>
    <dbReference type="FunCoup" id="C5DKQ0">
        <property type="interactions" value="190"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKQ0"/>
    <dbReference type="GeneID" id="8292689"/>
    <dbReference type="KEGG" id="lth:KLTH0F06534g"/>
    <dbReference type="eggNOG" id="KOG0938">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026996_5_2_1"/>
    <dbReference type="InParanoid" id="C5DKQ0"/>
    <dbReference type="OMA" id="VWKIPRI"/>
    <dbReference type="OrthoDB" id="10259133at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030131">
        <property type="term" value="C:clathrin adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09251">
        <property type="entry name" value="AP-2_Mu2_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14836">
        <property type="entry name" value="AP2_Mu_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1170">
        <property type="entry name" value="Mu homology domain, subdomain B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050431">
        <property type="entry name" value="Adaptor_comp_med_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036168">
        <property type="entry name" value="AP2_Mu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043532">
        <property type="entry name" value="AP2_Mu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001392">
        <property type="entry name" value="Clathrin_mu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018240">
        <property type="entry name" value="Clathrin_mu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028565">
        <property type="entry name" value="MHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043512">
        <property type="entry name" value="Mu2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10529">
        <property type="entry name" value="AP COMPLEX SUBUNIT MU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00928">
        <property type="entry name" value="Adap_comp_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005992">
        <property type="entry name" value="Clathrin_mu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00314">
        <property type="entry name" value="CLATHRINADPT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49447">
        <property type="entry name" value="Second domain of Mu2 adaptin subunit (ap50) of ap2 adaptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00991">
        <property type="entry name" value="CLAT_ADAPTOR_M_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51072">
        <property type="entry name" value="MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0168">Coated pit</keyword>
    <keyword evidence="10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MHD" evidence="12">
        <location>
            <begin position="195"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR005992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR24051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="466" mass="52074" checksum="D73B253E410D1143" modified="2009-07-28" version="1">MLSALFIFSLRGELLISKHVRSSVPKSMSEIFRIQVINNLDVRSPVLTLGSTTFHHVKSPGNLWIVAVSRSNADSAAIWEFLYKLSALLEAFGLHSENELKEDFMTCYELLDIVLEDGVPVDTELSSVASKMSVKPSASAERINTFIESGNGGTNRILPVAQFLRARSSSSNLHDSHSKVPSNIPWRMNGIKYKKNEVFLNVNERISILVSKDGSILKSYVDGTVEATTHLSGMPVCRFGLNDSLSVSTPFGDNESPTTNKKAIPKAAAGSVMLEDCKFHQCVQLDKFQSERTINFIPPDGSFELMKYHVRENLNLPFKITPVVTLFKANSIDYRVTIKSLFPSKLTAKDVQLRIPVPPETVDCHISTSNGRCKFVPEESAIIWKFSKYQGLTENSLSATAVPMKDSALNIDQWSKPPMSLKFEIVMFSNSGLVVRFFDVSEGDRNYKMVKWIKYLSKSGAYEVRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKQ8</accession>
    <name>C5DKQ8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0F06710p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0F06710g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554496.1">
        <property type="nucleotide sequence ID" value="XM_002554450.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKQ8"/>
    <dbReference type="FunCoup" id="C5DKQ8">
        <property type="interactions" value="4119"/>
    </dbReference>
    <dbReference type="GeneID" id="8292697"/>
    <dbReference type="KEGG" id="lth:KLTH0F06710g"/>
    <dbReference type="eggNOG" id="ENOG502RBAK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038620_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKQ8"/>
    <dbReference type="OMA" id="KYADPSL"/>
    <dbReference type="OrthoDB" id="7295497at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="386"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="189"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="300"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="189"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="300"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="331"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="344"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="373"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR24059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="479" mass="53423" checksum="26CE73B482258821" modified="2009-07-28" version="1">MTTTTELNPLTRTLTDVLDDELYHIANRGQECVQTPKGWKNRSLENLMYAPDQSTAFGTGVTFDADHEFKDSMFSKYADPSLTTMSLDKPVDSRKTSPASLTPVQATVSLNNVMNVNPFLARLPSNGQDVRISSPALFQGEDEDMLNSGESFPPDYYYEDNQKLFSWPLQESATMSQDAMSIFDREFDDDLSEDEDEDEDEELEAGDDQDQPKLEAGSEYQLNDDDSLIFDRVRPGASFVDDDRNDFAVVDDEDDEDDEEIGFHNELQSAGCTEGKHGEQDTFDEDVLYDRNNRKSSVVCSGEQYTTAPSSTRISRESSPEDTFTEAKCVTPSPSSLSSTPSFTRKKKYPSRRKSSNATPSAPAIKKRISPLPSVTSSPGSGQEVHTCTLVNPITNEPCSKKFSRPYDLIRHQKTIHASKKKVFRCVICIQQQGTEGYQKTFSRGDALSRHVKVKHELTGAEAQKAIQFAKENVEFAGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKX4</accession>
    <name>C5DKX4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Octanoyltransferase</fullName>
            <ecNumber evidence="5">2.3.1.181</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0F08272g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>octanoyl-[ACP] + L-lysyl-[protein] = N(6)-octanoyl-L-lysyl-[protein] + holo-[ACP] + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17665"/>
            <dbReference type="Rhea" id="RHEA-COMP:9636"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:9928"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78463"/>
            <dbReference type="ChEBI" id="CHEBI:78809"/>
            <dbReference type="EC" id="2.3.1.181"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 5">Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the LipB family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.181" evidence="5"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554562.1">
        <property type="nucleotide sequence ID" value="XM_002554516.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKX4"/>
    <dbReference type="FunCoup" id="C5DKX4">
        <property type="interactions" value="453"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKX4"/>
    <dbReference type="GeneID" id="8292768"/>
    <dbReference type="KEGG" id="lth:KLTH0F08272g"/>
    <dbReference type="eggNOG" id="KOG0325">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035168_0_1_1"/>
    <dbReference type="InParanoid" id="C5DKX4"/>
    <dbReference type="OMA" id="FEMCGLP"/>
    <dbReference type="OrthoDB" id="19908at2759"/>
    <dbReference type="UniPathway" id="UPA00538">
        <property type="reaction ID" value="UER00592"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033819">
        <property type="term" value="F:lipoyl(octanoyl) transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16444">
        <property type="entry name" value="LipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000544">
        <property type="entry name" value="Octanoyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020605">
        <property type="entry name" value="Octanoyltransferase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00214">
        <property type="entry name" value="lipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10993:SF7">
        <property type="entry name" value="LIPOYLTRANSFERASE 2, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10993">
        <property type="entry name" value="OCTANOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21948">
        <property type="entry name" value="LplA-B_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016262">
        <property type="entry name" value="LPLase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01313">
        <property type="entry name" value="LIPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="9">
        <location>
            <begin position="109"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="6">
        <location>
            <position position="271"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="163"/>
            <end position="170"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="240"/>
            <end position="242"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="253"/>
            <end position="255"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Cys" evidence="8">
        <location>
            <position position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR016262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR016262-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR016262-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR016262-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR24125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="323" mass="36330" checksum="5AEE50DA564ADFD6" modified="2009-07-28" version="1">MIKNARKPILRANVPYISRWRVKHSSTCSNKTLTHPVEESSKVLRHLEFIKPLEFQKGLQIQEKFVRAQLDMKALESKIKRKLGLLELETNGAPINEHEQKILDTIMDMKPNPIVLTFEFEPTYTGGKRSKKAVSDEDILNFENFRPKDQVKNKSPKFVQAERGGQVTFHGPGQVVAYIVMDLKSFHSFPAKCLVSAIEGSTIDTLKSAPQGKHKTPLNLQAIRTEETGVWIDEENKIASIGVHVRRSITSHGVSINVNPDLSYMNSFTMCGLPETRATSVLEQIPDTTCSTHDIAVTFVNKLAKKLGITTVERIQLDDIDID</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>C5DL92</accession>
    <name>C5DL92_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0F11022p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0F11022g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PHF5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554680.1">
        <property type="nucleotide sequence ID" value="XM_002554634.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DL92"/>
    <dbReference type="FunCoup" id="C5DL92">
        <property type="interactions" value="981"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DL92"/>
    <dbReference type="GeneID" id="8292893"/>
    <dbReference type="KEGG" id="lth:KLTH0F11022g"/>
    <dbReference type="eggNOG" id="KOG1705">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110369_1_1_1"/>
    <dbReference type="InParanoid" id="C5DL92"/>
    <dbReference type="OMA" id="AYYCWEC"/>
    <dbReference type="OrthoDB" id="10248186at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005345">
        <property type="entry name" value="PHF5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13120">
        <property type="entry name" value="PHD FINGER-LIKE DOMAIN-CONTAINING PROTEIN 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03660">
        <property type="entry name" value="PHF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016468">
        <property type="entry name" value="PHF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="107" mass="12116" checksum="C6C5BE321267A1B6" modified="2009-07-28" version="1">MSRHQFDLVMCLKQPGTHIGKLCDKCDGKCPICDSYVRPKSKVRICEQCSFGANWKKCIICGQMGTSDAFYCWECCRLEKNKDGCPKILNVGSNRTDKHFEKKASAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLE5</accession>
    <name>C5DLE5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0F12386p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0F12386g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554733.1">
        <property type="nucleotide sequence ID" value="XM_002554687.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLE5"/>
    <dbReference type="FunCoup" id="C5DLE5">
        <property type="interactions" value="49"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLE5"/>
    <dbReference type="GeneID" id="8292951"/>
    <dbReference type="KEGG" id="lth:KLTH0F12386g"/>
    <dbReference type="eggNOG" id="KOG2913">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049047_1_1_1"/>
    <dbReference type="InParanoid" id="C5DLE5"/>
    <dbReference type="OMA" id="QMSLDIY"/>
    <dbReference type="OrthoDB" id="292213at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052241">
        <property type="entry name" value="SLC66/Scramblase_ANY1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14856:SF9">
        <property type="entry name" value="PQ-LOOP REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14856">
        <property type="entry name" value="PQ-LOOP REPEAT-CONTAINING PROTEIN 1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="63"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="162"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="199"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="231"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="266"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="292"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR24296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="341" mass="39793" checksum="CF6E0E234D576C7F" modified="2009-07-28" version="1">MAAEDVNVPGNNPGSGSYFPRIDQFYIPEWLTMQFVLNNMICFTPLISYGTTVLSIKKSKTALGFSIDICATMLLASILRISYYLITPYDITLLRQSLVMIFIQLILLHTSLRYRPEEYKYENLEPVEPLTQLLHDVWLEYFPLNPFGPDWRLLIKSLSYKSFFDFAYKILLVLVYKFLKFFDPSYRRFRSFWQWNENSRFWLFLAYFSTAQITITLFAAKVMNWDSLAQWIGSTIGSLGLLIESLLPLPQISILNKLKSTQGFKLILLVSWLCGDVVKISYLVFGANNISALFLFFALFQMSLDFYIAFQYVYYRFYYPNTIAVPQNEEVELKTFDSAST</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLF1</accession>
    <name>C5DLF1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">thioredoxin-dependent peroxiredoxin</fullName>
            <ecNumber evidence="3">1.11.1.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Nuclear thiol peroxidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Thioredoxin peroxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">KLTH0F12562g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a hydroperoxide + [thioredoxin]-dithiol = an alcohol + [thioredoxin]-disulfide + H2O</text>
            <dbReference type="Rhea" id="RHEA:62620"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:35924"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.11.1.24"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the peroxiredoxin family. BCP/PrxQ subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.24" evidence="3"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554739.1">
        <property type="nucleotide sequence ID" value="XM_002554693.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLF1"/>
    <dbReference type="FunCoup" id="C5DLF1">
        <property type="interactions" value="46"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLF1"/>
    <dbReference type="GeneID" id="8292959"/>
    <dbReference type="KEGG" id="lth:KLTH0F12562g"/>
    <dbReference type="eggNOG" id="KOG0855">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_950184_0_0_1"/>
    <dbReference type="InParanoid" id="C5DLF1"/>
    <dbReference type="OMA" id="SHWIFAD"/>
    <dbReference type="OrthoDB" id="338622at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008379">
        <property type="term" value="F:thioredoxin peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03017">
        <property type="entry name" value="PRX_BCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000157">
        <property type="entry name" value="DOT5p Nuclear thiol peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050924">
        <property type="entry name" value="Peroxiredoxin_BCP/PrxQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42801:SF23">
        <property type="entry name" value="PEROXIREDOXIN DOT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42801">
        <property type="entry name" value="THIOREDOXIN-DEPENDENT PEROXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="9" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="15">
        <location>
            <begin position="44"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="170"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="202"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="256"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="276"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00077538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAR24302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="293" mass="32241" checksum="8485C9D0D0CDCC28" modified="2009-07-28" version="1">MVELRRSTRKKSSDAPKRAPEEEEVAPNAPETKKAKTNGSKGELEEGDEVPDVVLKNQEGEEVSLKSVVQQNKVVVVFAYPKASTPGCTKQACGFRDNYEELKKNAAVFGLSTDTPNAQQKFKEKNSLPFDLLSDPQRKLIGPLGASKSPSGTKRSYWVFVQGKLRTKRISVSPEASVSEAKKEAIEAAQTDDGENAKEEVKEEQDGEVSGEDEVDGNDEDDEDFNEQEGEDPELEEVGQEEDEEYEEGDLKKDEDSEADVEGGNDDSVDAKEEADGLQEDEAEGEEENESNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLN9</accession>
    <name>C5DLN9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0G02266p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0G02266g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
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    </organism>
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        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
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                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
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        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
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                <person name="Segurens B."/>
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                <person name="Fulton R.S."/>
                <person name="Minx P."/>
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                <person name="Jean G."/>
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                <person name="Martin T."/>
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        </source>
    </evidence>
    <sequence length="1545" mass="169562" checksum="C02829C2C260E3CC" modified="2009-07-28" version="1">MSEDQEELNLNELVGNLLAAHNEEAEAPQLEQGSLDELDEIPELPEQTGSQDAELGDDDLAAVVAQAIGGMEEPESQRSAEATTELHGDQIQELSEEKDQPQSKEEEWAQILQQGLLQESQQQHPDQLEESLNTGDGANENLDHEDEALRRAILDSLQALSVGENQGVPTKSHDEPKVKHKSKSKDTHKDKSKEKKSKKKDAKKPTKKKSSKKKKDSLKKNKEAPKSSSLTRQGGDDDILNFEDVIKGFMEQGAVEPADQETTNASGRRTAGTGDATDAETQAFVEATLRAFESELLSGTTSAPSTKTKSTQKKPLLAEDRSVVASTHGHKTTRIPSHNSSASSSKKKSSESSKKKKSSKGDSRRKEGYNEDDFSRALAEMVNQVVNTSISDVQPRVLTQPEGPLVPQGALPHTAVEPASDQQNTERTEAADAMGGAGDISMLNNSQLLQEHESFGADSTSNTFPPSGASAGTQVGNEVLDTALPEGGSISRNPDVDLVSLTEGLDLNQIMQNAMALAFQGQSGEQMNQSAVDDFNRQLGGINLSQLLDSSIGSTKAKKKAAKASKKKDATEKSSSKLQSPKKHSSKSKKHGVSNQELAAALEAGSFSIPGFTQGLPKTLLTAKPEKLASSKKSTSAPQVSERQWRKKFKAAATEAAAKARRQRMDRNKAQRSKMKEERRIARQDKQLKKKERQEKEEAERKELEIIVAKGPPYPPDLRLTKRGRPKKPFRRYTAEEMKKRASMPSLIDTESSKRVKKDRKKKERKPKKIPLSALRKIPLFNFIKKQMPLGLQSRLNDIDGTLARIPLGGSRELPSRENTHPLLSSALQNHLFNSLQNAVFDDKTAEREHEIETVETQKTIIHREKCSFHPPWAIPEHPPFVLPTARRKKRGEDVDGSLRKSSKDRKHRSKSKGANFVSGSKIIPASLFPIINTLKAAAMARAAAGATPEEASRHLGNMLRTARMTIAQTLANARGQGNRNYGSLKNFDDIKQMQEKDQALKKIPIFSLSSIKAIKDDSKKDSSQTAEPSTIPQNDDSKAVPAVNEVSSNTGEMQPETTSSPPKQVSSEAVADEKEVSEHDPTAAIGSRLTSPSDKEDQVSPPPKIGLRQNDEDHEAVKSQSSVDSDEKYLVGKNQAEEISQNAEPKKAESEGQGSAPKEVSSDLTKPQTGITLIKTETGVRLPPEEDGPRANSKEGLSQPDDELPAEIKSELTRAINDLIIPYPKVKKRYTKTPPVLNLEGLVPPGSIPLFKSENSDIRTIGGQRETTNTANIDTTTKRANPAKQPSVVETKYEFSLPTKNIRGEPLRAISILRRAKDYLTGEELTKLKKCITNERKRKWREANAKKNKNHDLRARLKKRANALFGEQDSTAKSEWCETEYTKRAVKVENESGEALAQGSSEPTTSVSDAEVLNMIASTFNKESVSRSIEKDINDEANLILADKKPTKKRTTAPTRKPIKIDAETTVPASDALHEGNNFRENNTKIAGEPNAGEPIDPIFMQMGKRGREEGHENDTNGAKRAKTKGCLGANILRRPAYINLENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>C5DM75</accession>
    <name>C5DM75_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0G06578p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0G06578g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555323.1">
        <property type="nucleotide sequence ID" value="XM_002555277.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DM75"/>
    <dbReference type="FunCoup" id="C5DM75">
        <property type="interactions" value="60"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DM75"/>
    <dbReference type="GeneID" id="8293592"/>
    <dbReference type="KEGG" id="lth:KLTH0G06578g"/>
    <dbReference type="eggNOG" id="KOG4562">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081930_0_0_1"/>
    <dbReference type="InParanoid" id="C5DM75"/>
    <dbReference type="OMA" id="FMDINAI"/>
    <dbReference type="OrthoDB" id="205198at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1210">
        <property type="entry name" value="MAGE homology domain, winged helix WH2 motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041899">
        <property type="entry name" value="MAGE_WH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002190">
        <property type="entry name" value="MHD_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01454">
        <property type="entry name" value="MAGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MAGE" evidence="2">
        <location>
            <begin position="26"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR24886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="308" mass="34665" checksum="B52FE8C466BF9CF8" modified="2009-07-28" version="1">MSDSESEVNDYGNEKTIVVAKRVLRRFLSLCESQSTIISRTKLSTIIREVSSEENTRTVKFGAIYKAMNSLLQNSFGYQLWGLYSKPPKNTKGGKRPVPSRDHTPEINEINSGGGAVNLKERAQFFMLVSELPKAPTAFRSLLLEEGTKLYDDRIVDDDYIGNDLSMLPEDSFENAVATDESLVAQGLTFVVLVLILFSKNSILHQELVQHLLKFGIPQDGQKIPVLDITLNDFLSLLIKREYIHRTDERAQDGAQEVSFYRIGRKTQLEFDKKSLLAMCFKIMEPDAAQMAQLEKSIDLSIADAYGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMB3</accession>
    <name>C5DMB3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">NADH-cytochrome b5 reductase</fullName>
            <ecNumber evidence="15">1.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">KLTH0G07436g</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13 15">
            <text>2 Fe(III)-[cytochrome b5] + NADH = 2 Fe(II)-[cytochrome b5] + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46680"/>
            <dbReference type="Rhea" id="RHEA-COMP:10438"/>
            <dbReference type="Rhea" id="RHEA-COMP:10439"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14 15">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion outer membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.6.2.2" evidence="15"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555361.1">
        <property type="nucleotide sequence ID" value="XM_002555315.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMB3"/>
    <dbReference type="FunCoup" id="C5DMB3">
        <property type="interactions" value="363"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMB3"/>
    <dbReference type="GeneID" id="8293631"/>
    <dbReference type="KEGG" id="lth:KLTH0G07436g"/>
    <dbReference type="eggNOG" id="KOG0534">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003827_9_1_1"/>
    <dbReference type="InParanoid" id="C5DMB3"/>
    <dbReference type="OMA" id="KGPEMQK"/>
    <dbReference type="OrthoDB" id="432685at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090524">
        <property type="term" value="F:cytochrome-b5 reductase activity, acting on NADH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06183">
        <property type="entry name" value="cyt_b5_reduct_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000032">
        <property type="entry name" value="NADH-cytochrome b5 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.80:FF:000009">
        <property type="entry name" value="NADH-cytochrome b5 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001834">
        <property type="entry name" value="CBR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008333">
        <property type="entry name" value="Cbr1-like_FAD-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19370">
        <property type="entry name" value="NADH-CYTOCHROME B5 REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19370:SF171">
        <property type="entry name" value="NADH-CYTOCHROME B5 REDUCTASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00970">
        <property type="entry name" value="FAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00406">
        <property type="entry name" value="CYTB5RDTASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14 15" id="KW-0274">FAD</keyword>
    <keyword evidence="4 14 15" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="10 15" id="KW-0520">NAD</keyword>
    <keyword evidence="9 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="FAD-binding FR-type" evidence="16">
        <location>
            <begin position="43"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR601834-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU361226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAR24924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="292" mass="32523" checksum="45C56DA49FB08F7C" modified="2009-07-28" version="1">MFSRVLRSRSAIPLAVGSVTIAAAFYHSTLIRNEPLKTFTGDDSWVELPIAKIEEVSHDTRRFIFSLPQEEQVSGLVVASALLAKLQTPKGSNVIRPYTPVSDVSTKGHIEFVIKHYNGGKMTEMLFDKKPSDTVAFKGPIPKWEWKPNSFDSITLLGAGSGITPLYQLVHHISQDPKDKTKVHLLYGNKTPNDILLKRELDAIQAKYPDQVKIHYFVDKAEGSFDGHTGFITKEYLEKNVPKASEKNQVFVCGPPPFMKAYSGPKASPKDQGELTGILKDLGFTKESVFKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMB5</accession>
    <name>C5DMB5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial</fullName>
            <ecNumber evidence="16">1.3.5.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ordered locus">KLTH0G07480g</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 16">
            <text>a quinone + succinate = fumarate + a quinol</text>
            <dbReference type="Rhea" id="RHEA:40523"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.3.5.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="14">Flavinylated by SdhE, about 5% flavinylation occurs in the absence of SdhE.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 16">Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (eukaryal route): step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Mitochondrion inner membrane</location>
            <topology evidence="16">Peripheral membrane protein</topology>
            <orientation evidence="16">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.5.1" evidence="16"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555363.1">
        <property type="nucleotide sequence ID" value="XM_002555317.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMB5"/>
    <dbReference type="FunCoup" id="C5DMB5">
        <property type="interactions" value="774"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMB5"/>
    <dbReference type="GeneID" id="8293633"/>
    <dbReference type="KEGG" id="lth:KLTH0G07480g"/>
    <dbReference type="eggNOG" id="KOG2403">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014312_6_1_1"/>
    <dbReference type="InParanoid" id="C5DMB5"/>
    <dbReference type="OMA" id="PTGIWRM"/>
    <dbReference type="OrthoDB" id="71672at2759"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER01006"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008177">
        <property type="term" value="F:succinate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006121">
        <property type="term" value="P:mitochondrial electron transport, succinate to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.700.10:FF:000001">
        <property type="entry name" value="Mitochondrial succinate dehydrogenase flavoprotein subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.80.40:FF:000002">
        <property type="entry name" value="Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000026">
        <property type="entry name" value="Succinate dehydrogenase flavoprotein subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.100:FF:000001">
        <property type="entry name" value="Succinate dehydrogenase flavoprotein subunit (SdhA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.100">
        <property type="entry name" value="Fumarate reductase/succinate dehydrogenase flavoprotein-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.80.40">
        <property type="entry name" value="succinate dehydrogenase protein domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.700.10">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-dep_OxRdtase_2_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003952">
        <property type="entry name" value="FRD_SDH_FAD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037099">
        <property type="entry name" value="Fum_R/Succ_DH_flav-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015939">
        <property type="entry name" value="Fum_Rdtase/Succ_DH_flav-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030664">
        <property type="entry name" value="SdhA/FrdA/AprA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027477">
        <property type="entry name" value="Succ_DH/fumarate_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011281">
        <property type="entry name" value="Succ_DH_flav_su_fwd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014006">
        <property type="entry name" value="Succ_Dhase_FrdA_Gneg"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01816">
        <property type="entry name" value="sdhA_forward"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01812">
        <property type="entry name" value="sdhA_frdA_Gneg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11632">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE 2 FLAVOPROTEIN SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11632:SF51">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE [UBIQUINONE] FLAVOPROTEIN SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02910">
        <property type="entry name" value="Succ_DH_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000171">
        <property type="entry name" value="SDHA_APRA_LASPO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46977">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56425">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00504">
        <property type="entry name" value="FRD_SDH_FAD_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7 14 16" id="KW-0274">FAD</keyword>
    <keyword evidence="6 14 16" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 16" id="KW-0813">Transport</keyword>
    <keyword evidence="5 16" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="FAD-dependent oxidoreductase 2 FAD-binding" evidence="18">
        <location>
            <begin position="52"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="domain" description="Fumarate reductase/succinate dehydrogenase flavoprotein-like C-terminal" evidence="19">
        <location>
            <begin position="502"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="571"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="574"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="329"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="57"/>
            <end position="62"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="80"/>
            <end position="95"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="297"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="396"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="430"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="441"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="446"/>
            <end position="447"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="Tele-8alpha-FAD histidine" evidence="15">
        <location>
            <position position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000171-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR611281-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR611281-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR611281-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU362051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CAR24926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="637" mass="69516" checksum="121EB6909E23B5C8" modified="2009-07-28" version="1">MLSVRQMKLSTSRRLFSRTSRALRAFAANGDVDGQNSAPFGGQYQVVDHEYDCLVVGAGGAGLRAAFGLAEAGYKTACISKLFPTRSHTVAAQGGINAALGNMHKDNWKWHMYDTVKGSDWLGDQDSIHYMTREAADSIIELEHFGVPFSRTEEGRIYQRAFGGQTKENGKGGQAYRTCAVADRTGHALLHTLYGQALRHNTHFFIEYFALDLLVHNGEVVGVIAYNQEDGTIHRFRAHKTVIATGGYGRAYFSCTSAHTCTGDGNAMVSRAGFPLQDLEFVQFHPSGIYGSGCLITEGARGEGGFLVNSEGERFMERYAPTAKDLACRDVVSRAITMEIREGRGVGPEKDHMYLQLNHLPASVLKERLPGISETAAIFAGVDVTKEPIPILPTVHYNMGGIPTKWTGEALTVDEEGNDQVIPGLMACGEAACVSVHGANRLGANSLLDLVVFGRAVAHTVADTLQPGLPHKPLPSDLGKESIANLDKLRNATGSKPTSQIRLAMQKAMQADVSVFRTQESLDEGVRNVGAIDESFEDVGTTDRSMIWNSDMVETLELQNLLSNARQTAVSAANRKESRGAHAREDYPERDDKEWMKHTLSWQKHPQGNVELGYRSVIATTLDESECPPVLPTVRAY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-05-31" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMK0</accession>
    <name>YNF8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName>Vacuolar membrane protein KLTH0G09570g</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">KLTH0G09570g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PRM5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555448.1">
        <property type="nucleotide sequence ID" value="XM_002555402.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMK0"/>
    <dbReference type="FunCoup" id="C5DMK0">
        <property type="interactions" value="91"/>
    </dbReference>
    <dbReference type="GeneID" id="8293726"/>
    <dbReference type="KEGG" id="lth:KLTH0G09570g"/>
    <dbReference type="eggNOG" id="ENOG502S625">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061224_0_0_1"/>
    <dbReference type="InParanoid" id="C5DMK0"/>
    <dbReference type="OMA" id="MEGYHKR"/>
    <dbReference type="OrthoDB" id="4065319at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051009">
        <property type="entry name" value="PRM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36089">
        <property type="entry name" value="CHITIN SYNTHASE 3 COMPLEX PROTEIN CSI2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36089:SF1">
        <property type="entry name" value="CHITIN SYNTHASE 3 COMPLEX PROTEIN CSI2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0926">Vacuole</keyword>
    <feature type="chain" id="PRO_0000409318" description="Vacuolar membrane protein KLTH0G09570g">
        <location>
            <begin position="1"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="123"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="226"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="274"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="298" mass="32462" checksum="D152612BCBCCF60F" modified="2009-07-28" version="1">MVHQTDFISSTAILKTQKASTSLVHATMFERAMPSLTNAGKASSTKTYTTPTITPPSIKGNPHIWSSDKPSGTLFIAVGSVVGFIFLIIALAYIVSAYISRRQTEKLRFETIDQEFQSHVGGKSYSKLGNSDDPEKSGFLSKAVHTPQSRSVARLLDRPDFQQPSPALSNQDSSTSLAQEFYSSIRDQTAAQNRKSLFISPTVEIVNQQRKSAVLQNMNNSVSSLVSDSGAELNKPEKAAPSTRKAMYKARNKSSMGSAVGIAKSRSTSPVKSGLRDKPLDRAKTPSVYLDKMFEDES</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMK5</accession>
    <name>C5DMK5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0G09680p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0G09680g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555453.1">
        <property type="nucleotide sequence ID" value="XM_002555407.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMK5"/>
    <dbReference type="FunCoup" id="C5DMK5">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMK5"/>
    <dbReference type="GeneID" id="8293731"/>
    <dbReference type="KEGG" id="lth:KLTH0G09680g"/>
    <dbReference type="eggNOG" id="ENOG502QU5B">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006002_0_0_1"/>
    <dbReference type="InParanoid" id="C5DMK5"/>
    <dbReference type="OMA" id="IVRGNMY"/>
    <dbReference type="OrthoDB" id="1204at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070772">
        <property type="term" value="C:PAS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010513">
        <property type="term" value="P:positive regulation of phosphatidylinositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903778">
        <property type="term" value="P:protein localization to vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000011">
        <property type="term" value="P:vacuole inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024260">
        <property type="entry name" value="Vac7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28258">
        <property type="entry name" value="VACUOLAR SEGREGATION PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28258:SF1">
        <property type="entry name" value="VACUOLAR SEGREGATION PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12751">
        <property type="entry name" value="Vac7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="477"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="288"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="300"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="310"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR25016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="681" mass="75229" checksum="2A97A547DF18E4D6" modified="2009-07-28" version="1">MSTEDDKRMIVETETVQTPAQNLLPVQSEDKAEDEATSIITQGDGPANLIDHNPLSIHNGSGAIRNKKSSLLIDSFLGKEATPSSTGLASTQGTAKPAVQSTSHSKPSHKELLEESQPRLGVPEDHKPEALSPRASGPIEHAEDGTARRARDPPNKADFFAARLASAVGENEISDSEETFVYESTANSTNNAAPGESRAYGIPAKMSAPLLNHNTPNASTSKLLNRAKATRHTSIAAIPPAQPSEQSDDLHSLRYVNRANPQEIRSVRSYTTMPRSPGKRLSLISLAQNGGASAPRKTSRLASSNSNANARRKVSQKRQLRTTASKIFDAHGASLRRYSGVPDDVNLEDYIEQTGGELTPKKFAYHSDSEFEDEPVSYTQNADNEEEDNHSMFYYSHPKNDLEARHVLSDYDEAEEGGHPVQGQHLGYQPELDYYADNYYLQPTEATALKKKPSHYNGYSPHNFYTTKTNWARFRNFVYFAFVVSFLLTLGFISGFLLATNKELHDLQLSKITSVLVSADELVFDVIATAFNPGFFTVEIQKVELDIFAKTSHLDPDGGALGTPYETILLGTVENLENSLQFQGGFFRRNYDHSKSSVKLIHPGSVDEEGRDDARPHKENIEDGIAKWRQLIHYEFDLIVRGNMYYKMPFFNSEKSVGVQCVTLVNPYKGGNGNELLGNEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DML3</accession>
    <name>C5DML3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0G09856p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0G09856g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555461.1">
        <property type="nucleotide sequence ID" value="XM_002555415.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DML3"/>
    <dbReference type="FunCoup" id="C5DML3">
        <property type="interactions" value="65"/>
    </dbReference>
    <dbReference type="GeneID" id="8293739"/>
    <dbReference type="KEGG" id="lth:KLTH0G09856g"/>
    <dbReference type="eggNOG" id="KOG4525">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009601_2_1_1"/>
    <dbReference type="InParanoid" id="C5DML3"/>
    <dbReference type="OMA" id="MFRNNFG"/>
    <dbReference type="OrthoDB" id="74460at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.100.10.30">
        <property type="entry name" value="Jacalin-like lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001229">
        <property type="entry name" value="Jacalin-like_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036404">
        <property type="entry name" value="Jacalin-like_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053002">
        <property type="entry name" value="Metalloproteinase_M10B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021917">
        <property type="entry name" value="Unchr_Zn-peptidase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21054:SF2">
        <property type="entry name" value="MIP04191P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21054">
        <property type="entry name" value="ZINC METALLOPROTEINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01419">
        <property type="entry name" value="Jacalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12044">
        <property type="entry name" value="Metallopep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51101">
        <property type="entry name" value="Mannose-binding lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51752">
        <property type="entry name" value="JACALIN_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Jacalin-type lectin" evidence="1">
        <location>
            <begin position="521"/>
            <end position="689"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR25024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="690" mass="76837" checksum="1003FD31D333AFD8" modified="2009-07-28" version="1">MGEIVLYNLQDGVEVYSACLVIHGKCSSSNTSVIEVRHSQFPCLRYEVHKSGFKCLVQLSPGENKLTFATDQKESKTVTCHYTPLVQNEPVHLCLLVARDSPMVFDSPSSQQKKEGGNNLDLAVRKLRLGARLMQAYTNEQMLRNGFGPRTFQFVEEYGLDTTFKQPEMRNRVKVHIVRTEKSVKELRDPDRAQQNPHAKDAGGLFGIAMEALRKYGGPFASSSERPVQAAVMILDAHWDAKLNLITAHAALGGGDDSLKLAIFGSHGLYAWPYFIEDVPRYFTDETRSSTKEVANDCNECGSHWECFTLTLGAFMHEIGHLLGCPHQENGVMLRDYVRLNRSFLSKEAFSTRTNSNGAQPPIYPREECTWHRLDLLKFLYHPSFTLPQDFYDPSMMRPGCIGGFKAPSPSVYVLPNNCCVLRSETGIYCIQIICGDLARAHIEYLPISLGGTGPQKEIFLSLDDLRSRIPPEHLEKYRDSFKIKACAVNSPESNWEQFPRFLQASQIPMANYGFPKHVVGMKSSLYGGADRGKDVGIIAFDGRRVTAVRVFHGAALDGIMLFLDEGNSNPEKDPPLPPRTYMGKLTNAMKSVSIQSTDRQSVLFGKQTNSYSDFEMNFGEHLLGFNLRSGCWVDGVQVLTTQGRASKFFGNNGGGSQHQIIAPGGQHILGFYGRVGQWVDAIGIVYGAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMM3</accession>
    <name>C5DMM3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Conserved oligomeric Golgi complex subunit 6</fullName>
            <shortName evidence="10">COG complex subunit 6</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 10">Component of oligomeric Golgi complex 6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0G10076g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Acts as a component of the peripheral membrane COG complex that is involved in intra-Golgi protein trafficking. COG is located at the cis-Golgi, and regulates tethering of retrograde intra-Golgi vesicles and possibly a number of other membrane trafficking events.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Acts as component of the peripheral membrane COG complex that is involved in intra-Golgi protein trafficking. COG is located at the cis-Golgi, and regulates tethering of retrograde intra-Golgi vesicles and possibly a number of other membrane trafficking events.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the conserved oligomeric Golgi complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Golgi apparatus membrane</location>
            <topology evidence="1 10">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the COG6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555471.1">
        <property type="nucleotide sequence ID" value="XM_002555425.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMM3"/>
    <dbReference type="FunCoup" id="C5DMM3">
        <property type="interactions" value="294"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMM3"/>
    <dbReference type="GeneID" id="8293749"/>
    <dbReference type="KEGG" id="lth:KLTH0G10076g"/>
    <dbReference type="eggNOG" id="KOG3758">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017837_0_0_1"/>
    <dbReference type="InParanoid" id="C5DMM3"/>
    <dbReference type="OMA" id="IINMICP"/>
    <dbReference type="OrthoDB" id="272987at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017119">
        <property type="term" value="C:Golgi transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010490">
        <property type="entry name" value="COG6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048369">
        <property type="entry name" value="COG6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048368">
        <property type="entry name" value="COG6_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21506">
        <property type="entry name" value="COMPONENT OF OLIGOMERIC GOLGI COMPLEX 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21506:SF0">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20653">
        <property type="entry name" value="COG6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06419">
        <property type="entry name" value="COG6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01087">
        <property type="entry name" value="COG6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Conserved oligomeric complex COG6 N-terminal" evidence="12">
        <location>
            <begin position="174"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="Conserved Oligomeric Golgi complex subunit 6 C-terminal" evidence="13">
        <location>
            <begin position="306"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="14"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00043873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF06419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF20653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR25034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="813" mass="93539" checksum="8BE8129D9001F1B6" modified="2009-07-28" version="1">MDFLDYQTFALDEANAKDYDLPEPASRLPIKSQQSSKDLDKDFSMPRLANAELDAEGTENLQDKMQKYASLSLSALSLKRLPPQSSMENPVVSQGTSLGEKTLEQLVQNGEKTIDNTDALLSKRLSRILDAHHRNTLQCDTQLRRSFKVLEDNQDRLHFNSKTLVRPDFVGSLARNSLRSNLESELLKSHLMILDDIQPIVRRIKRLSEPVQNIHQMGDALLKQGEMAHDETKTYMDTVYILQNALLKHRLKKRILVMLRDKLTLNQVEEDALINSNVGPEFFEVMKKLMRLKETATYLLALPNPKAGTVLMEQTNAKIEKANKRIFNYLVDFLYDLQSASKTFGERAFSSNDKPLITFQQGMLYLSNDLPIFNEFIKRVVKARSQIVLDDFLSQFDVSSAKASRPIIISAHDPVRYLGDVLAHVHSLIVDEADFMDSMFKFQNWNIQDTPTSILQENREFLNGLSISLLNETFGPVENSIRIRLEQIIRFEDDPIINFEIYELLALYQMMFIKYGLRPDSNLILQLINLRNTSSSKILTGLTKILDDANITQNLEKELLPAEWLVKYLAKLCELLTKFEKSGSVKDSETLIDSNFLKEALACLITEKLPEYHKSKFPQARKEKEVKKHLLLSEINCIDLIKTRMLPYRETIFSKGDRHLVYENIDSHLQASTKSLIGLETKSLLEKLGLDLYSNLFNMIFPVESVQDELDYDMYLPVLENPIMQRDTIRSNVHIKLNDFLPILLSDIQDNLLFHIVSPKIADHISTECLSCLSKFYKVFRQVLLKLYPENHEDIDTILNFSEEEFDTLIGLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMT1</accession>
    <name>C5DMT1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Small nuclear ribonucleoprotein Sm D3</fullName>
            <shortName evidence="9">Sm-D3</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">snRNP core protein D3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0G11396g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the snRNP core protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555529.1">
        <property type="nucleotide sequence ID" value="XM_002555483.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMT1"/>
    <dbReference type="FunCoup" id="C5DMT1">
        <property type="interactions" value="1272"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMT1"/>
    <dbReference type="GeneID" id="8293808"/>
    <dbReference type="KEGG" id="lth:KLTH0G11396g"/>
    <dbReference type="eggNOG" id="KOG3172">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099537_1_2_1"/>
    <dbReference type="InParanoid" id="C5DMT1"/>
    <dbReference type="OMA" id="HTITCET"/>
    <dbReference type="OrthoDB" id="6425924at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01721">
        <property type="entry name" value="Sm_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000002">
        <property type="entry name" value="Small nuclear ribonucleoprotein Sm D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027141">
        <property type="entry name" value="LSm4/Sm_D1/D3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034099">
        <property type="entry name" value="SmD3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23338">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="Sm" evidence="11">
        <location>
            <begin position="6"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="90"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR25092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="102" mass="11297" checksum="443F7AC3ADE7EA02" modified="2009-07-28" version="1">MSSNGIPIKLLTESQGHVVSLELTTGEAYRGKLVDSEDNMNVQLRDVTATARDGSVTRMDHVFVRGSHIRFFVVPDILKNAPMFKQGPTSKPPPPIRGPKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMW1</accession>
    <name>C5DMW1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0G12122p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0G12122g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M28 family. M28B subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555559.1">
        <property type="nucleotide sequence ID" value="XM_002555513.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMW1"/>
    <dbReference type="STRING" id="559295.C5DMW1"/>
    <dbReference type="GeneID" id="8293841"/>
    <dbReference type="KEGG" id="lth:KLTH0G12122g"/>
    <dbReference type="eggNOG" id="KOG2195">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005688_2_0_1"/>
    <dbReference type="InParanoid" id="C5DMW1"/>
    <dbReference type="OMA" id="RNGMIAR"/>
    <dbReference type="OrthoDB" id="5841748at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08022">
        <property type="entry name" value="M28_PSMA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02121">
        <property type="entry name" value="PA_GCPII_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000008">
        <property type="entry name" value="Glutamate carboxypeptidase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000101">
        <property type="entry name" value="N-acetylated alpha-linked acidic dipeptidase like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.40">
        <property type="entry name" value="Transferrin receptor-like, dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039373">
        <property type="entry name" value="Peptidase_M28B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007365">
        <property type="entry name" value="TFR-like_dimer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036757">
        <property type="entry name" value="TFR-like_dimer_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10404">
        <property type="entry name" value="N-ACETYLATED-ALPHA-LINKED ACIDIC DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10404:SF46">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04253">
        <property type="entry name" value="TFR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47672">
        <property type="entry name" value="Transferrin receptor-like dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PA" evidence="3">
        <location>
            <begin position="167"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M28" evidence="5">
        <location>
            <begin position="356"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="domain" description="Transferrin receptor-like dimerisation" evidence="4">
        <location>
            <begin position="625"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="234"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="248"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR25122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="744" mass="82956" checksum="DB2067AA42DAB81D" modified="2009-07-28" version="1">MVASDVEKAIAASSDNSRRSTRRWGVVLQLTIVALLLMRRLHFPGPGELGIASPLESAFLGALDENLAGNWSEKYTSIPHLAGQGVELVNWTAEKFREYGLLTDVESFDIYLNYPVEHGLQLLKDGRNGTRVEYTATLEEDRLPDDPTTDGDDLVQAFHGYSASGNVTAEYVYVNYGTKEDFELLKSLKVDLTGKIAVARYGKIFRGLKVKFAQEAGCVGVLIYSDPGDDYYRESKGDKAYPDGPARNPSSLQRGSVQFLSQNPGDPTTPGYASQGDVERVDPYDSIPSIPSLPISMREVEPILKRLNGHGLNVTSIGGDKWVGALKGFDYWTGPAPGYSLNLYNNQSYDIRPIYNVYGNISGTDAGGEYILVGNHRDAWIKGGASDPNSGSASMLEVIRAFSQLMEQGWKPRKTIVFASWDGEEYALLGSTEFGEKYADDLKANCLAYLNVDVSVSGKTLNVASSPLLNYVLEEALHLVQYPEGGTLHDHYFGRKEKFGILGSGSDYTVFQEHLGIASVDMGFDSSFKEPVYHYHSNYDSFHWMSTMCDPGFKLHNALARYLGLVTLKLSERKVTGFKVFDYAAELDLYFEELKDRVPIGWLNIPAGRCKKTIADALVILGDRLGGLKKRAASFDARAEELQALWDEPMPWWKRIALHYRIVGLNYKLRFLERMFLHEEGLEGRPWFKHVVYAAGRDTGYEGFAFPGLKEALDDDDPVAFGKWLRIIGRTIKHLEKRLGHAKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>C5DN40</accession>
    <name>C5DN40_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0G13948p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0G13948g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555638.1">
        <property type="nucleotide sequence ID" value="XM_002555592.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DN40"/>
    <dbReference type="FunCoup" id="C5DN40">
        <property type="interactions" value="21"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DN40"/>
    <dbReference type="GeneID" id="8293922"/>
    <dbReference type="KEGG" id="lth:KLTH0G13948g"/>
    <dbReference type="eggNOG" id="ENOG502SG4R">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1008557_0_0_1"/>
    <dbReference type="InParanoid" id="C5DN40"/>
    <dbReference type="OrthoDB" id="6067455at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="251"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="253"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR25201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="276" mass="31063" checksum="BA8E2C705D59175D" modified="2009-07-28" version="1">MADTHNRGGHHLQPHDENELSVLRPAATKVVLNKKTFFREVHVSDHQYELQTLHTNTGSSVVPTAATTTQLSLPVNGLRVPKPLHLKDYASPPALTGKKRPLDPISTFGTVEEGPTTDLLRPFIPITVRNDKLLPDGESLDLSSLANELPTSVDFPPFLHLEEYEKPLDRDDYEGLCLAALQDEFMRIATGEGPVFDRQLEIEEKEGQLSFLEYPLYYLFGVDRTGYFELKEERDRFQSCPWSRFANIKKQSETTNSESESALTSASIESSLEFLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNC0</accession>
    <name>C5DNC0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">KLTH0G15752p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0G15752g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CSC1 (TC 1.A.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555718.1">
        <property type="nucleotide sequence ID" value="XM_002555672.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNC0"/>
    <dbReference type="FunCoup" id="C5DNC0">
        <property type="interactions" value="162"/>
    </dbReference>
    <dbReference type="GeneID" id="8294004"/>
    <dbReference type="KEGG" id="lth:KLTH0G15752g"/>
    <dbReference type="eggNOG" id="KOG1134">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002458_2_1_1"/>
    <dbReference type="InParanoid" id="C5DNC0"/>
    <dbReference type="OMA" id="NWACVAL"/>
    <dbReference type="OrthoDB" id="1076608at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005227">
        <property type="term" value="F:calcium-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045122">
        <property type="entry name" value="Csc1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003864">
        <property type="entry name" value="CSC1/OSCA1-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027815">
        <property type="entry name" value="CSC1/OSCA1-like_cyt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032880">
        <property type="entry name" value="CSC1/OSCA1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022257">
        <property type="entry name" value="PHM7_ext"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018:SF139">
        <property type="entry name" value="PHOSPHATE METABOLISM PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018">
        <property type="entry name" value="PROBABLE MEMBRANE PROTEIN DUF221-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14703">
        <property type="entry name" value="PHM7_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12621">
        <property type="entry name" value="PHM7_ext"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02714">
        <property type="entry name" value="RSN1_7TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13967">
        <property type="entry name" value="RSN1_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="93"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="143"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="390"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="435"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="480"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="533"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="606"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="641"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="669"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like N-terminal transmembrane" evidence="11">
        <location>
            <begin position="13"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like cytosolic" evidence="12">
        <location>
            <begin position="187"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like 7TM region" evidence="9">
        <location>
            <begin position="387"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="domain" description="10TM putative phosphate transporter extracellular tail" evidence="10">
        <location>
            <begin position="865"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="753"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="758"/>
            <end position="768"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF12621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF13967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF14703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR25281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="964" mass="108853" checksum="2333AD037C75C9EC" modified="2009-07-28" version="1">MADSDPPNASTSAFVSSLIFYGIIALIFVLVFVMLRPKEPRVYQPRALRDVITVREEERTEEVPEGPFAWLGYLISRPHSFLIQHAGIDGYLFLRYVAVFGGLSMIGCLMLFPILLPVNATNGHNFSGFEILSFANVKNNNRYYAHVFLSWFFFGLVIYVIFRELYHFVSLRHAIQSSPLYDGLISSRTVVLTELSADLQNEAEIQKRFQNCSNVVFARDNSELQALVQERAKLANKYEGCMNKVIKKSLKIRAKAEKKGKLDELIGSKPEDDLQTYIPQNKRPTHRLGKIPLPIIGGEKVDTLEYCSKRIGELNDDIKEKQQAWDTQDKVGACFLEFKGQLDAQRAYQSVKYILDKGSYDQCMIGCPPEDLTWANMGLSKKARKSKRTVANTVLVLMIIFWAIPVAVVGFISNINFLIETLKFLEFIKKLPKVLLGLITGLLPTVALAILMSLVPIFIKKLGSISGCMSIQEQELYCQAWYYAFNVVQVFIVFTCASSAVSTIKSIMDDPSSAMTLLAANLPKSSNFYINYYILQGLSFSSGTLAQLVNLILSRFLGRILDSTPRQKWARYNTLSKPTWGVLYPNMELLVCILICYAFVQPIILLFSTICLGLFYIAYIYSFNYVMGFSFDLRGRNYGRALFQVFLGLYLAEICLLGLFIMAKTWGPVVLEAVFIAFTALCHLYFQRRFLPLFDCVPLSAIRYARGESTFFYPTKDQGLSEIKSTAEQFKHEQDENETGGVLQPATSVELKKAGILSDDEGEPEKEEDTEKKRQSTSTSKTGANVEEPNFVPEDEEFHKYSYRDVADLDTNNNDMERVDANGAVVTNADAGNVFSDANAVTQDPKAFPPSQTGQQSWKDTIVGFFSPQKSYDFSAVRARLPYVFNNTVQYDKEYLETAYTDPCVTDPEPILWIAKDPLGLSTQQILLARTHSVKVSDEFTAYDDKNRATFSFNPPDYEPVAKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNM3</accession>
    <name>C5DNM3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0G18304p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0G18304g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555821.1">
        <property type="nucleotide sequence ID" value="XM_002555775.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNM3"/>
    <dbReference type="FunCoup" id="C5DNM3">
        <property type="interactions" value="208"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNM3"/>
    <dbReference type="GeneID" id="8294116"/>
    <dbReference type="KEGG" id="lth:KLTH0G18304g"/>
    <dbReference type="eggNOG" id="KOG0872">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047036_3_0_1"/>
    <dbReference type="InParanoid" id="C5DNM3"/>
    <dbReference type="OMA" id="GPGLWYN"/>
    <dbReference type="OrthoDB" id="6354873at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050307">
        <property type="entry name" value="Sterol_Desaturase_Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11863">
        <property type="entry name" value="STEROL DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="84"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="130"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="173"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="245"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid hydroxylase" evidence="6">
        <location>
            <begin position="177"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR25384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="357" mass="42068" checksum="4B2031A8F93080DB" modified="2009-07-28" version="1">MDLVLEFFDSYGLDYVYSRTLPAQLASKFPAQWQSALSLNQGLGNLTTVGTSNGDVYGYAPYLFKMSDYTFQSLLPRYNVLRQFLSLTVITTIFGWLLYFSVATFSYVFIFDKAVFNHPRYLKNQMSLEIKQAMSAIPFMVLLTVPWFLLELHGHSKLYMTVDTQNHGIRQLLLEYVYFLMFTDCGIYLLHRWLHWPRVYKALHKPHHKWLVTTPYASHAFHPVDGYFQSLPYHVYPMLFPLNKVSYLILFTFVNFWTVMIHDGEYLANDPVVNGAACHTVHHLYFNYNYGQFTTLWDRLGGSYREPDRELFNKSLKKSTKTWEEQIKKMEVIKKQVEGEDDGYRVYGTEEKFKKMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5E254</accession>
    <name>C5E254_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0H02244p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0H02244g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the large ribosomal subunit (LSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes). uL29 is associated with the polypeptide exit tunnel.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uL29 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555977.1">
        <property type="nucleotide sequence ID" value="XM_002555931.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E254"/>
    <dbReference type="FunCoup" id="C5E254">
        <property type="interactions" value="1007"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E254"/>
    <dbReference type="GeneID" id="8294290"/>
    <dbReference type="KEGG" id="lth:KLTH0H02244g"/>
    <dbReference type="eggNOG" id="KOG3436">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110381_0_1_1"/>
    <dbReference type="InParanoid" id="C5E254"/>
    <dbReference type="OMA" id="VMNQKAR"/>
    <dbReference type="OrthoDB" id="528635at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00427">
        <property type="entry name" value="Ribosomal_L29_HIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.310:FF:000002">
        <property type="entry name" value="60S ribosomal protein L35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.250.3450:FF:000001">
        <property type="entry name" value="60S ribosomal protein L35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00374">
        <property type="entry name" value="Ribosomal_uL29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001854">
        <property type="entry name" value="Ribosomal_uL29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045059">
        <property type="entry name" value="Ribosomal_uL29_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036049">
        <property type="entry name" value="Ribosomal_uL29_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00012">
        <property type="entry name" value="L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45722">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45722:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL29-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00831">
        <property type="entry name" value="Ribosomal_L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46561">
        <property type="entry name" value="Ribosomal protein L29 (L29p)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="7"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00064105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR30115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="120" mass="13947" checksum="CFD571AE07248879" modified="2009-07-28" version="1">MAGIKAYELRTKSKEQLESQLLDLKKELAALKVQKLSRPSLPKIHTVRKDIARVLTIVNENQRNAVKELYKGAKYQPKDMRAKKTRALRRALTKFEASRTTEKQRKKQIAFPQRKFAIKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5E289</accession>
    <name>C5E289_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0H03014p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0H03014g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556012.1">
        <property type="nucleotide sequence ID" value="XM_002555966.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E289"/>
    <dbReference type="FunCoup" id="C5E289">
        <property type="interactions" value="75"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E289"/>
    <dbReference type="GeneID" id="8294325"/>
    <dbReference type="KEGG" id="lth:KLTH0H03014g"/>
    <dbReference type="eggNOG" id="KOG0504">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000134_20_0_1"/>
    <dbReference type="InParanoid" id="C5E289"/>
    <dbReference type="OMA" id="EAENVGW"/>
    <dbReference type="OrthoDB" id="10057496at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0085020">
        <property type="term" value="P:protein K6-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171:SF8">
        <property type="entry name" value="BRCA1-ASSOCIATED RING DOMAIN PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="49"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="89"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="146"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="162"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR30150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="197" mass="22016" checksum="86707C85F8D64704" modified="2009-07-28" version="1">MSLHSDPLDQEDQDAIILDARCGDLESLKDIFTNLVDPKLIVTCRDAVTQSTPLHMAAANGHKEVMQYLASLVTDGAELKKWVNSQNETGNTALHWASLNGSLECVKFLCEELGADPFIRNNFDHDAIFEAERGNKEEVENFYPQKYDVEPENDETAQAESVEIKEGTEIEQVTKEATEALRLETDKLSLSEDRGQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-04-02" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2A8</accession>
    <name>C5E2A8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0H03454p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0H03454g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556031.1">
        <property type="nucleotide sequence ID" value="XM_002555985.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2A8"/>
    <dbReference type="GeneID" id="8294344"/>
    <dbReference type="KEGG" id="lth:KLTH0H03454g"/>
    <dbReference type="HOGENOM" id="CLU_2015685_0_0_1"/>
    <dbReference type="InParanoid" id="C5E2A8"/>
    <dbReference type="OrthoDB" id="4036665at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="41"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR30169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="123" mass="12013" checksum="92B75D722E4116A1" modified="2009-07-28" version="1">MSDLFERARQRLSGSSSAVPEKLRDLYMSHGPRDASGGSGSMSRRMTGADDGAGPGSSALSSGGDSSGDDSPGASSSDLASNGSRDLAHSHMVSHARGGSDNAAADAGAETGSGADPAANATN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2J4</accession>
    <name>C5E2J4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">KLTH0H05478p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0H05478g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the USE1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556117.1">
        <property type="nucleotide sequence ID" value="XM_002556071.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2J4"/>
    <dbReference type="FunCoup" id="C5E2J4">
        <property type="interactions" value="167"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2J4"/>
    <dbReference type="GeneID" id="8294432"/>
    <dbReference type="KEGG" id="lth:KLTH0H05478g"/>
    <dbReference type="eggNOG" id="KOG2678">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093583_0_0_1"/>
    <dbReference type="InParanoid" id="C5E2J4"/>
    <dbReference type="OMA" id="DSIMNTH"/>
    <dbReference type="OrthoDB" id="4008582at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15860">
        <property type="entry name" value="SNARE_USE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019150">
        <property type="entry name" value="Vesicle_transport_protein_Use1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13050">
        <property type="entry name" value="USE1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13050:SF7">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN USE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09753">
        <property type="entry name" value="Use1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="249"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR30255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="276" mass="30841" checksum="2D17E63503EF1AFE" modified="2009-07-28" version="1">MVMSVLPQLQTHKAMYGGPFDNWKRALEPVIAEELSFSDDPVLQDIISTKALANLALLRKSAVESQLRAPKHGSSELDDYRNAFEELECSALMTRHSSRAKLTAEHEHLMESMKSKRYSVDFDSEDTGRQEEDFENIEKAAGPEGEEEESLGQLRSRLLGNRRGSSSGADQSANTEKQMQVHNNLQEELISDMSQLVSGLKMGAEAFQNALEEDSTVLKATELGLQATSRSLTNLGGKLKKYHNSKVGLLFYLGCILFMFLSLLLTYMIVKIFPKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2L2</accession>
    <name>C5E2L2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14 15">mRNA cap guanine-N(7) methyltransferase</fullName>
            <ecNumber evidence="3 15">2.1.1.56</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 15">mRNA (guanine-N(7))-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12 15">mRNA cap methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ordered locus">KLTH0H05874g</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Responsible for methylating the 5'-cap structure of mRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a 5'-end (5'-triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:67008"/>
            <dbReference type="Rhea" id="RHEA-COMP:17166"/>
            <dbReference type="Rhea" id="RHEA-COMP:17167"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:156461"/>
            <dbReference type="ChEBI" id="CHEBI:167617"/>
            <dbReference type="EC" id="2.1.1.56"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 15">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the class I-like SAM-binding methyltransferase superfamily. mRNA cap 0 methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.56" evidence="3 15"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556135.1">
        <property type="nucleotide sequence ID" value="XM_002556089.1"/>
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            <name>mRNA</name>
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    <sequence length="413" mass="48121" checksum="FE5E8CC4EED553A4" modified="2009-07-28" version="1">MNVRPEKPVWMSQEQYDKQYGSPNETIVKKPTPKPAEEEQSKVEEVEQEGDDDKKELEKPVFKIQKRKHQRYDSEERRKKHQLHKMREEQIKRHEIDMTANKVVNVDQIIRKHYNERTFVAKRRRRHLSPIIKLRNFNNAIKYMLIDKFTFPGNVVLEMGCGKGGDLRKYGAAGISQFIGIDISNASIVEAQKRFSSMGNLDYQVILITGDCFGESLGVAVEPFPECRFPCDVVSAQFCLHYAFESEEKARRTLLNVTKSLKIGGYFIGTIPDSEFIRYKLNKITKDVDKPSWGNAIYKVTFENSDYQKNNNEFTSPFGQMYTYWLEDAIDNVPEYVIPFETLRSLADEYGLELELQMPFNAFFVQEIPKWINKFSPKMQEGLQRSDGKYGVEGDEKEAASYFYTVFAFKKVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2P5</accession>
    <name>C5E2P5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Ubiquitin-like protein ATG12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0H06644g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Ubiquitin-like protein involved in cytoplasm to vacuole transport (Cvt), autophagy vesicles formation, mitophagy, and nucleophagy.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Forms a conjugate with ATG5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Preautophagosomal structure membrane</location>
            <topology evidence="1 8">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ATG12 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_002556122.1"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106795_0_0_1"/>
    <dbReference type="InParanoid" id="C5E2P5"/>
    <dbReference type="OMA" id="NIGELWM"/>
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        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034274">
        <property type="term" value="C:Atg12-Atg5-Atg16 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:autophagosome membrane"/>
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        <property type="term" value="C:phagophore assembly site membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:Atg8-family ligase activity"/>
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        <property type="term" value="P:autophagy of mitochondrion"/>
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    </dbReference>
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    </dbReference>
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        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
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    </dbReference>
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        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Ubl_ATG12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007242">
        <property type="entry name" value="Atg12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13385">
        <property type="entry name" value="AUTOPHAGY PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13385:SF0">
        <property type="entry name" value="UBIQUITIN-LIKE PROTEIN ATG12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04110">
        <property type="entry name" value="APG12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0072">Autophagy</keyword>
    <keyword evidence="4 8" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <keyword evidence="5 8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="52"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="57"/>
            <end position="68"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004623"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00007778"/>
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            <dbReference type="ARBA" id="ARBA00015875"/>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR30306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="194" mass="20742" checksum="4C16442B344FE04C" modified="2009-07-28" version="1">MSQLLESESDAASTSSTGESSRFGKASYSVQDGLEQYSKRLSFLGLGSSDDVPALEDNSSGADASSGNIKAEPAPKRSAPVSTSAVLTHLTPAGDQAIKRFTEKEGILPEKVQIKFQPIGSVSQVLPQSARISASQPFSVLVTFLRKKLKMNSVYCYVNNSFSPAPQQSVGDLWRHFRVNDELVVSYCGGVAFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2T9</accession>
    <name>C5E2T9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0H07656p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0H07656g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556212.1">
        <property type="nucleotide sequence ID" value="XM_002556166.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2T9"/>
    <dbReference type="FunCoup" id="C5E2T9">
        <property type="interactions" value="94"/>
    </dbReference>
    <dbReference type="GeneID" id="8294530"/>
    <dbReference type="KEGG" id="lth:KLTH0H07656g"/>
    <dbReference type="HOGENOM" id="CLU_180256_0_0_1"/>
    <dbReference type="InParanoid" id="C5E2T9"/>
    <dbReference type="OrthoDB" id="4034641at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR30350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="96" mass="10692" checksum="59E2F5E6D5E3070B" modified="2009-07-28" version="1">MLQQTTRGTRMLAGVAVRAATPRASQRFLSRSCALQMPAGKSDMTQKDMDANKKKLDELEKKSHNGGAYKRPSTDELKKKGEDAQVEQHRPDDGVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2Y6</accession>
    <name>C5E2Y6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0H08866p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0H08866g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556259.1">
        <property type="nucleotide sequence ID" value="XM_002556213.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2Y6"/>
    <dbReference type="FunCoup" id="C5E2Y6">
        <property type="interactions" value="123"/>
    </dbReference>
    <dbReference type="GeneID" id="8294584"/>
    <dbReference type="KEGG" id="lth:KLTH0H08866g"/>
    <dbReference type="eggNOG" id="ENOG502S6WH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_180937_0_0_1"/>
    <dbReference type="InParanoid" id="C5E2Y6"/>
    <dbReference type="OMA" id="TYRHFAH"/>
    <dbReference type="OrthoDB" id="202195at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010530">
        <property type="entry name" value="B12D"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06522">
        <property type="entry name" value="B12D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR30397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="78" mass="8946" checksum="FF6FA79587CE7569" modified="2009-07-28" version="1">MRPTSLLLNAAKKQKSGFDIPVELTPLFVAMGVAVSSACWFTYKKFRWDESLRVGRKNPDRSGLKTILEEPEKFEKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5E305</accession>
    <name>C5E305_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0H09284p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0H09284g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556278.1">
        <property type="nucleotide sequence ID" value="XM_002556232.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E305"/>
    <dbReference type="FunCoup" id="C5E305">
        <property type="interactions" value="35"/>
    </dbReference>
    <dbReference type="GeneID" id="8294603"/>
    <dbReference type="KEGG" id="lth:KLTH0H09284g"/>
    <dbReference type="eggNOG" id="KOG4526">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059211_2_0_1"/>
    <dbReference type="InParanoid" id="C5E305"/>
    <dbReference type="OMA" id="VHSMGQE"/>
    <dbReference type="OrthoDB" id="426386at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045866">
        <property type="entry name" value="FAM210A/B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009688">
        <property type="entry name" value="FAM210A/B-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21377">
        <property type="entry name" value="PROTEIN FAM210B, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21377:SF0">
        <property type="entry name" value="PROTEIN FAM210B, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06916">
        <property type="entry name" value="FAM210A-B_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="89"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1279" evidence="3">
        <location>
            <begin position="82"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="134"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR30416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="263" mass="30059" checksum="6C047E75CE674487" modified="2009-07-28" version="1">MLTFRNNKRSIYEIRSLLTKDQIENLSQFITHKQPPNHMSLFTRFSRLACSKAIRSPALRWPSNIASSRFASTATKSEKPSLKNLMKTYGSSALVVYFGLSAIDLPICFFAVHSLGEETIRVYINRAKQIFGYGRDEQELIKDVRAQIEEKEQRKLSGEEQHNTSLWERLKESTILTEFLIAYGIHKSLIILRLPVTAAITPATVKLLQKWGFNIGKFNKSFQTAGDVAKFKNKTGRPEDFVKGNPIPKQTQTKGRKWFNGLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>C5E337</accession>
    <name>C5E337_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0H10032p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0H10032g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556310.1">
        <property type="nucleotide sequence ID" value="XM_002556264.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E337"/>
    <dbReference type="FunCoup" id="C5E337">
        <property type="interactions" value="109"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E337"/>
    <dbReference type="GeneID" id="8294637"/>
    <dbReference type="KEGG" id="lth:KLTH0H10032g"/>
    <dbReference type="eggNOG" id="KOG0715">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030116_0_0_1"/>
    <dbReference type="InParanoid" id="C5E337"/>
    <dbReference type="OMA" id="DMDYKPC"/>
    <dbReference type="OrthoDB" id="1726119at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051787">
        <property type="term" value="F:misfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051727">
        <property type="entry name" value="DnaJ_C3_Co-chaperones"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44140">
        <property type="entry name" value="LD25575P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44140:SF2">
        <property type="entry name" value="LD25575P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="J" evidence="5">
        <location>
            <begin position="503"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="460"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="561"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="460"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="562"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="575"/>
            <end position="587"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR30448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="607" mass="68739" checksum="EDE55CE40ACF7F07" modified="2009-07-28" version="1">MIRWIFYVAVGIAHAAGKCDFGRLSGASHELRYDDSEIPQYQKLCEQLSHCDHPEAEALRNQLLYKSGLIQVSSGQELAAAETLEQILGSSDVKFQSQASKRLDEIYLQFGFWDKLKGEGGKKQEYEALRNAVVVQLSRNQAVPHESLAQLEEIAPFALETRILLNDALLRELGETLDINAAQSIIENYKIILTKHKKLVNVQDKLRIHHAVAVLQMFVMATPPTNLLSCLALDMDYKPCRELSKLSSKLNRINPPLNAVTHPDQFLSLSQFDWAKLVDFYLNAPSIKFTGTSSAKNNFDLLSSIQRQLLQNLLSERPLSLIKNKWKLGKRKSAFTTNQNQLVCEGYDAMHKSKLAEPYCQAALGEKLPENSAKALSEFTDNFQNQQAVQDVLNSLWQDSPSLAAHAVRKIVSSLAAHEKNQKSGNTAQVWFFLESFTKNHNWAQSSNSGLSKIAKHISSISAKRKQQQQQQQQQQQQQHFFRQQRQQQRQPPPAPNDMSKKNYYKVLEVTPQATSKDIRKSYLSMTRKYHPDKQGQLSESQKLQNQERMSEINEAYEILSDEGKRKEYDDQRAGRGQQRQGFPFGQGANGGFPFGGGSFKMNFGGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5E384</accession>
    <name>C5E384_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0H11176p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0H11176g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cullin family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556357.1">
        <property type="nucleotide sequence ID" value="XM_002556311.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E384"/>
    <dbReference type="FunCoup" id="C5E384">
        <property type="interactions" value="129"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E384"/>
    <dbReference type="GeneID" id="8294688"/>
    <dbReference type="KEGG" id="lth:KLTH0H11176g"/>
    <dbReference type="eggNOG" id="KOG2166">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_357505_0_0_1"/>
    <dbReference type="InParanoid" id="C5E384"/>
    <dbReference type="OMA" id="MVQACIL"/>
    <dbReference type="OrthoDB" id="27073at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.130">
        <property type="entry name" value="Cullin, Chain C, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045093">
        <property type="entry name" value="Cullin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059120">
        <property type="entry name" value="Cullin-like_AB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016158">
        <property type="entry name" value="Cullin_homology"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036317">
        <property type="entry name" value="Cullin_homology_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11932">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26557">
        <property type="entry name" value="Cullin_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75632">
        <property type="entry name" value="Cullin homology domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50069">
        <property type="entry name" value="CULLIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cullin family profile" evidence="2">
        <location>
            <begin position="404"/>
            <end position="655"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR30495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="774" mass="89449" checksum="FC721B2F1DE7FC87" modified="2009-07-28" version="1">MQNGEGLLTFDVVSLQFRNAVDELLRRSFDKKLRTLDGPNLLSTIDRIFYYELSRRLARSKIYVPTATEVSGEDTTLRKKAKAFRLLSQEEVVIKIWHLIIEELDKSVDDVVSELAEKLTLESEPKAAFVRHWPSLCLGQFEVQVSLLAEAFHLFAEDFSFIFNAFTGVHTIREFAEYRLVESALSVLGNEAEGALTEALTEIRKQYHNCDEEGARKAENYNRTGTECLKKFVEVVGKCHLRFTHHRKSFRVFYLDNLEFVLSKTNMEIDKDYLKNVKNLLIREDMIVNLISKSIHLETKKIILTKFLFSAGVLENLLEIYALQRGFVREFKMIKLAYNSDNRCNEFYASLANVIGIRFGNSFQRQRTLDEVLSYTCSLYDLEDDKVPQLVKRGLELAVDGELNLVEPLVKSLDSKVKGCYKRLKEPQSVGLQQAKKDYEIFLSLWRILESFDLLEPFFKLYMERSLFRRMIIMGTEYLAYMNHPENLEKRFFDLLEARQSHWELVTQLKALRSNLIDTQKLFQGFETQELQNVQLAPMIFERKNVPASFHDFTFPELKIPRSLDALWQKFCAFYAESDPRSTKKPLVLQNMLHHLEVQTNFKLEDGRPLMLELTLLQASILDLFNESGELTVRDIESELGVAANILEPALTSFISAGLLNTDGGSFIVNEEFKPDIKKMKDGRLKIFHKAAPKAALKPPEDGLEKKDTSWIKEVLRARIVRVLKTSSASFSFEQLKTAVEKEKSGFSMGEFKTALAECKDFYSLNKELYQYKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3G4</accession>
    <name>C5E3G4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0H13332p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0H13332g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556437.1">
        <property type="nucleotide sequence ID" value="XM_002556391.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3G4"/>
    <dbReference type="FunCoup" id="C5E3G4">
        <property type="interactions" value="64"/>
    </dbReference>
    <dbReference type="GeneID" id="8294796"/>
    <dbReference type="KEGG" id="lth:KLTH0H13332g"/>
    <dbReference type="eggNOG" id="ENOG502SDJV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093897_2_0_1"/>
    <dbReference type="InParanoid" id="C5E3G4"/>
    <dbReference type="OMA" id="GKSLNCW"/>
    <dbReference type="OrthoDB" id="5544375at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012471">
        <property type="entry name" value="DUF1690"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07956">
        <property type="entry name" value="DUF1690"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR30575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="153" mass="17000" checksum="AE4C49934BA25326" modified="2009-07-28" version="1">MGSQPSKPAETKVFTPKTQVDFTSTLLAQLEQSTEGDFSRQQLANKYLEQRVSEKLAQLEEETLKKFEDKLNTSLLADNGNSDSELSSKGLSEKVASLNSHLAKLKEAQSARSSDETLKSSKEALSKCLRDNEGKPLNCFEEVQNFKKVALQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3J9</accession>
    <name>C5E3J9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">KLTH0H14190p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0H14190g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556472.1">
        <property type="nucleotide sequence ID" value="XM_002556426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3J9"/>
    <dbReference type="GeneID" id="8294834"/>
    <dbReference type="KEGG" id="lth:KLTH0H14190g"/>
    <dbReference type="eggNOG" id="ENOG502QWM5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034486_0_0_1"/>
    <dbReference type="InParanoid" id="C5E3J9"/>
    <dbReference type="OMA" id="SWRMTTP"/>
    <dbReference type="OrthoDB" id="3364892at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624:SF26">
        <property type="entry name" value="CARRIER PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G07710)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="152"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR30610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="441" mass="50383" checksum="26C31BE0D5B7543B" modified="2009-07-28" version="1">MSSEAVKNEDSALTGGSSSLIGAMTAGGRSMVYQLTSFYLRTPVKLFRPARFDYLHYLRVLLVGEDKAQTHIEKAPQSRFLKFWNPRYFYYLENSSIGLLTKGLNKYGWKVIPERVLPPLVANSVAGVVLYTTYLTTLDHFDKVNNPESTNHRPLDVMRAGFLAGAAQALTSAPIDAIYTRSTVDQLISHAKTYDNLWVYGFKKLQEIGLVGCFGGFGLSLVKECTGFAAYFTVFEVFKGQICQWTIDFMKHYRELKFSIRQSKIARLFQEESEEDLNDIDPVFMSTREESWFRKAFVFVGGVSAALLLQMIQYPFAKLQKMHLSRLEVLDMYSKAKIADEAKPATSTISPKPNHIRIKLRPKSRRLFHIYYHSYLDTFQHIYFIQKNTGSTLRWLYKGFTRNTLAIIPGTTAGLLLLEVMRNKLSENVSESSSKFSKFQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3K6</accession>
    <name>C5E3K6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="20 21">Cytochrome b-c1 complex subunit Rieske, mitochondrial</fullName>
            <ecNumber evidence="3 21">7.1.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ordered locus">KLTH0H14344g</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19 21">
            <text>a quinol + 2 Fe(III)-[cytochrome c](out) = a quinone + 2 Fe(II)-[cytochrome c](out) + 2 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:11484"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="7.1.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="21">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
        <text evidence="21">Binds 1 [2Fe-2S] cluster per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="21">The Rieske protein is a high potential 2Fe-2S protein.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Rieske iron-sulfur protein family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.8" evidence="3 21"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556479.1">
        <property type="nucleotide sequence ID" value="XM_002556433.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3K6"/>
    <dbReference type="FunCoup" id="C5E3K6">
        <property type="interactions" value="828"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3K6"/>
    <dbReference type="GeneID" id="8294841"/>
    <dbReference type="KEGG" id="lth:KLTH0H14344g"/>
    <dbReference type="eggNOG" id="KOG1671">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055690_0_1_1"/>
    <dbReference type="InParanoid" id="C5E3K6"/>
    <dbReference type="OMA" id="KRTWLIA"/>
    <dbReference type="OrthoDB" id="1637982at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008121">
        <property type="term" value="F:quinol-cytochrome-c reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd03470">
        <property type="entry name" value="Rieske_cytochrome_bc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.270:FF:000002">
        <property type="entry name" value="Cytochrome b-c1 complex subunit Rieske, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.102.10.10:FF:000001">
        <property type="entry name" value="Cytochrome b-c1 complex subunit Rieske, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="entry name" value="Rieske [2Fe-2S] iron-sulphur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.270">
        <property type="entry name" value="Ubiquinol cytochrome reductase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037008">
        <property type="entry name" value="bc1_Rieske_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014349">
        <property type="entry name" value="Rieske_Fe-S_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005805">
        <property type="entry name" value="Rieske_Fe-S_prot_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004192">
        <property type="entry name" value="Rieske_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006317">
        <property type="entry name" value="Ubiquinol_cyt_c_Rdtase_Fe-S-su"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01416">
        <property type="entry name" value="Rieske_proteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10134">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT RIESKE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02921">
        <property type="entry name" value="UCR_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00162">
        <property type="entry name" value="RIESKE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="ISP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81502">
        <property type="entry name" value="ISP transmembrane anchor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12 21" id="KW-0249">Electron transport</keyword>
    <keyword evidence="14" id="KW-0408">Iron</keyword>
    <keyword evidence="15" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16 22" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 22" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="11" id="KW-1278">Translocase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 21" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Rieske" evidence="23">
        <location>
            <begin position="145"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00029351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00072517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU004494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU004495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="CAR30617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="214" mass="23248" checksum="07EAFD00FBCF915D" modified="2009-07-28" version="1">MLGLRTSLKGSLRALNKQSRLMSSSAVAAKSTYRTPDFGDYLKEKNDADKSRSYAYFMVGSLGLLSSVGAKSTVETFISTMSASADVLAMAKVEVNLAAIPEGKNVVVKWQGKPVFIRHRTQGEIDEANAVDMSALKDPQTDSDRVKNPEWLVMLGICTHLGCVPIGEAGDFGGWFCPCHGSHYDISGRIRKGPAPLNLEIPEYEFDDTKLIVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3P8</accession>
    <name>C5E3P8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0H15356p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0H15356g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556521.1">
        <property type="nucleotide sequence ID" value="XM_002556475.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3P8"/>
    <dbReference type="FunCoup" id="C5E3P8">
        <property type="interactions" value="64"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3P8"/>
    <dbReference type="GeneID" id="8294887"/>
    <dbReference type="KEGG" id="lth:KLTH0H15356g"/>
    <dbReference type="eggNOG" id="KOG4453">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031477_0_1_1"/>
    <dbReference type="InParanoid" id="C5E3P8"/>
    <dbReference type="OMA" id="TKHEVPR"/>
    <dbReference type="OrthoDB" id="5673at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004143">
        <property type="term" value="F:ATP-dependent diacylglycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037997">
        <property type="entry name" value="Dgk1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31303">
        <property type="entry name" value="CTP-DEPENDENT DIACYLGLYCEROL KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31303:SF1">
        <property type="entry name" value="CTP-DEPENDENT DIACYLGLYCEROL KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="62"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="84"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="126"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="184"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="255"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR30659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="275" mass="30885" checksum="AF08BDA85ABBDAAE" modified="2009-07-28" version="1">MVDVQTTSEGDPSASPQLKQRFKTQSKSESNSDEHLPLAQIRLKSHEWFGEFITKHEIPRKAFHSSIGFITLYLYTTGMDYRNVKWPLIVAFAILLPLDLIRLRWNAINKLYCRCVGALMRKSEIHTYNGVLWYLLGLIFAFSFFPKDVALISLFLLSWCDTAASTFGCKFGHLTPKLARNKSLAGSIAAFVVGFLVCLLFYGVFVPHYAYVNSPGELSWSAETSRLSLTQFSLLGGVVAALSEGIDLFNWDDNFTIPVLSAIFMNAVIRFAKAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-02-15" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>Q5K463</accession>
    <name>Q5K463_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative DNA endonuclase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">io-cox1.3</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <geneLocation type="mitochondrion" evidence="2"/>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2005" name="FEBS Lett." volume="579" first="30" last="40">
            <title>The complete mitochondrial genome of the yeast Kluyveromyces thermotolerans.</title>
            <authorList>
                <person name="Talla E."/>
                <person name="Anthouard V."/>
                <person name="Bouchier C."/>
                <person name="Frangeul L."/>
                <person name="Dujon B."/>
            </authorList>
            <dbReference type="PubMed" id="15620687"/>
            <dbReference type="DOI" id="10.1016/j.febslet.2004.10.106"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AJ634268">
        <property type="protein sequence ID" value="CAG25601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_184723.1">
        <property type="nucleotide sequence ID" value="NC_006626.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q5K463"/>
    <dbReference type="STRING" id="559295.Q5K463"/>
    <dbReference type="GeneID" id="3238988"/>
    <dbReference type="InParanoid" id="Q5K463"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Mitochondrion"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.28.10">
        <property type="entry name" value="Homing endonucleases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027434">
        <property type="entry name" value="Homing_endonucl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004860">
        <property type="entry name" value="LAGLIDADG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37520">
        <property type="entry name" value="INTRON-ENCODED DNA ENDONUCLEASE AI2A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37520:SF1">
        <property type="entry name" value="INTRON-ENCODED DNA ENDONUCLEASE AI2A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00961">
        <property type="entry name" value="LAGLIDADG_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55608">
        <property type="entry name" value="Homing endonucleases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homing endonuclease LAGLIDADG" evidence="1">
        <location>
            <begin position="82"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="Homing endonuclease LAGLIDADG" evidence="1">
        <location>
            <begin position="189"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAG25601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="314" mass="36102" checksum="A43025CD45CDE7DC" modified="2005-02-15" version="1">MLMKDNNQTVTNNTYHPHFVGMSYLVGTSETTRKVLINKSLRTLSTIFYSTTSSADGYDNNKYKDIPHRFAGEGDIKFNQWLAGLIDGDGYLGTSKGKYSSCEITLALEDEKALRQIQNKFGGSIKLRVGVKAIRYRLHNKEGMIKLINAINGNIRNSKRLVQLYQVCDLLNIKPINPIKLTNNNAWFTGFFDADGTINYSFKNGCPQLTISVTNKYSHDVEYYSEVFGGNIYFDKAKNGYFKWSIQSKLNVLNFINDYIKLYPSRTTKHNRLLLCKTFYELKELKAYNSPHNTALYKAWLNFEKKWNNKCTPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBA5</accession>
    <name>C5DBA5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0A00946p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0A00946g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551504.1">
        <property type="nucleotide sequence ID" value="XM_002551458.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBA5"/>
    <dbReference type="FunCoup" id="C5DBA5">
        <property type="interactions" value="101"/>
    </dbReference>
    <dbReference type="GeneID" id="8290295"/>
    <dbReference type="KEGG" id="lth:KLTH0A00946g"/>
    <dbReference type="eggNOG" id="ENOG502RYR8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071874_0_0_1"/>
    <dbReference type="InParanoid" id="C5DBA5"/>
    <dbReference type="OMA" id="NDNENMS"/>
    <dbReference type="OrthoDB" id="4053251at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013743">
        <property type="entry name" value="NBP1/CSA1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08537">
        <property type="entry name" value="NBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="231"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="279"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="165"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR21062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="301" mass="34970" checksum="A1C8F6BFF9BCA574" modified="2009-07-28" version="1">MEAAKNYFKSWFNADEPRKVERGSLDKWRKRSQMLDGLGGRGARRGRTSVTLRNKTTGKSLRPKRTAPGIWNLVKGVFSREDQDLREMKNAYTALKLRSNNRGMQTRSQVQRRIASSAALKRKLLEKQYDEKRLDELRRGRKPGPRAVALEPRNISQPNFDTDRVILLQHKVDALKEQLNEVERELHLTRKRLKFTQEKNALLEGLLNDANVDDDYLKSRRRITNLQREDLKPEYDALPPSPHRAISPLVTSSPRKVELPQDDFYERYRSIPTAELLRRDPESSLSPIRVDLSKYSSSRAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBA8</accession>
    <name>C5DBA8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0A01012p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0A01012g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551507.1">
        <property type="nucleotide sequence ID" value="XM_002551461.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBA8"/>
    <dbReference type="FunCoup" id="C5DBA8">
        <property type="interactions" value="131"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBA8"/>
    <dbReference type="GeneID" id="8290298"/>
    <dbReference type="KEGG" id="lth:KLTH0A01012g"/>
    <dbReference type="eggNOG" id="ENOG502QWCQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051401_0_0_1"/>
    <dbReference type="InParanoid" id="C5DBA8"/>
    <dbReference type="OMA" id="WEQPHRL"/>
    <dbReference type="OrthoDB" id="62853at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="CDD" id="cd05840">
        <property type="entry name" value="PWWP_ScIOC4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035503">
        <property type="entry name" value="IOC4-like_PWWP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000313">
        <property type="entry name" value="PWWP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00855">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00293">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50812">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PWWP" evidence="2">
        <location>
            <begin position="7"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR21065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="309" mass="34912" checksum="F3619C2E1EA9B2FC" modified="2009-07-28" version="1">MEQKLTTGDLVLCKVGSFAPWPAVVFPQRLLRKDVYRRRRAECVAVCFFNDETYYWDKPARLKRLTKSTIERFLEDAPATEDPGLVGAYEEARDYTSLHEFVMQRLEEENRIEEWEKSAEADTKVEAGQDPFEGRPAGLKAGGAKLRKSSASASPPRGYPKARRAASGSADGSIGTKSSSASLKSRSMPESPSAVKKLSRLDHAKRVEISLLFRCKLQRNLVQRELPPGPDEIKESHKLLGKITENTLANPQFFDFEALKHSKLHKLLKVITNDDNLAEFHRECNDILASWSPVIAELKKDKLNQRRDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBD6</accession>
    <name>C5DBD6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Coatomer subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">KLTH0A01694g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Oligomeric complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 11">Golgi apparatus membrane</location>
            <topology evidence="1 11">Peripheral membrane protein</topology>
            <orientation evidence="1 11">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Cytoplasmic vesicle</location>
            <location evidence="11">COPI-coated vesicle membrane</location>
            <topology evidence="11">Peripheral membrane protein</topology>
            <orientation evidence="11">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the COPG family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551535.1">
        <property type="nucleotide sequence ID" value="XM_002551489.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBD6"/>
    <dbReference type="FunCoup" id="C5DBD6">
        <property type="interactions" value="1342"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBD6"/>
    <dbReference type="GeneID" id="8290328"/>
    <dbReference type="KEGG" id="lth:KLTH0A01694g"/>
    <dbReference type="eggNOG" id="KOG1078">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010353_2_0_1"/>
    <dbReference type="InParanoid" id="C5DBD6"/>
    <dbReference type="OMA" id="DFIEDCE"/>
    <dbReference type="OrthoDB" id="1074925at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000368">
        <property type="entry name" value="Coatomer subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1480:FF:000001">
        <property type="entry name" value="Coatomer subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1480">
        <property type="entry name" value="Coatomer, gamma subunit, appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.10">
        <property type="entry name" value="TATA-Binding Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013041">
        <property type="entry name" value="Clathrin_app_Ig-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009028">
        <property type="entry name" value="Coatomer/calthrin_app_sub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032154">
        <property type="entry name" value="Coatomer_g_Cpla"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017106">
        <property type="entry name" value="Coatomer_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013040">
        <property type="entry name" value="Coatomer_gsu_app_Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037067">
        <property type="entry name" value="Coatomer_gsu_app_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012295">
        <property type="entry name" value="TBP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10261">
        <property type="entry name" value="COATOMER SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10261:SF0">
        <property type="entry name" value="COATOMER SUBUNIT GAMMA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16381">
        <property type="entry name" value="Coatomer_g_Cpla"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08752">
        <property type="entry name" value="COP-gamma_platf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037093">
        <property type="entry name" value="Coatomer_gamma_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49348">
        <property type="entry name" value="Clathrin adaptor appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55711">
        <property type="entry name" value="Subdomain of clathrin and coatomer appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 11" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6 11" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8 11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Clathrin/coatomer adaptor adaptin-like N-terminal" evidence="13">
        <location>
            <begin position="20"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="domain" description="Coatomer gamma subunit appendage Ig-like subdomain" evidence="14">
        <location>
            <begin position="669"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="domain" description="Coatomer subunit gamma C-terminal" evidence="15">
        <location>
            <begin position="820"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="630"/>
            <end position="664"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR037093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF16381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAR21093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="933" mass="103849" checksum="B3875E2224BD6EC9" modified="2009-07-28" version="1">MSTHTYKKFEEADSGILPDKMTIYQDCLNAFNESPVNAKRCRLLISRLLQLLAQGQTFPRTEATALFFAISKLFQHPNDSLRQAVYLAIKELSAVSDDVLMATSSIMKDVQNGSDMIKPNAIRSLTRVLDESTAFSAERLFKSAVVSKHPSISSAALVSSYHLLPVAESTVKRYANETQEAVSDLKTYPYQSGTSEHYPNSTYIAQYHALGLLYLLRKHDKVALMKLVQQFSAGNLLKNQLAQVQLVKLVGSLLQKDPQLVTQFTPLLLSWLSNKYESVQLEDAKLITSLPDRFVSPEIFASAVQTLQGLLTVPRVTTRFAAVRVLNRISMVAPEKIVVCNPELESLINDSNRNVSTYAITSLLKTGTSNNIASLIKTITNFIHEISDDFKIIIIDAVRTMALKFPEEWKSILNFLINVLKQGEGGFEFKNSIVEALFDLVQFVPQSRELAFENLCDFIEDCEYNEILVRILHILGKEGPSMKIPSLYVRHIYNRVVLENSIVRSAAVVALSKFALVKNDPTLVESIEILLRRISNDADDEVRDRATISLEFIESIKSKEGASSSAAEDLLQSKYSYDLVSLESKLSQYMSAKQESFKAPFDSSSVHRYTEDERKAIDLKRKQEQLFSALPSAGKSKRAASSSQDAEKRNGSYAGPASNEVDQDLQSTKFVEELESIEELKSLGPIINTSKEVPLTETEAEFVVSGVKHLFKNHVVLQFHITNTLTDVALDNVSVGCTMEQEDSTALQETSTIPVDRIMPSETFACYVVYEKSEEVVTEGFLNTLYFTTRELDATTHEPFAGDEGFQDEYEIDSLFLTAGDYIKGSFVGNFSACFDELPHSEVAVYNIKENISLQEVVDKLVVNTSCLTLDNTQFAPTDSNSHTLKLFGKSVLNGARVALVVRMIKSSKGIALKGEIRSDDANLCNDVVDGLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBQ9</accession>
    <name>C5DBQ9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein-lysine N-methyltransferase EFM4</fullName>
            <ecNumber evidence="7">2.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Elongation factor methyltransferase 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">EFM4</name>
        <name evidence="9" type="ordered locus">KLTH0A04642g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">S-adenosyl-L-methionine-dependent protein-lysine N-methyltransferase that mono- and dimethylates elongation factor 1-alpha at 'Lys-316'. May play a role in intracellular transport.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the class I-like SAM-binding methyltransferase superfamily. EFM4 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="7"/>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551658.1">
        <property type="nucleotide sequence ID" value="XM_002551612.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBQ9"/>
    <dbReference type="FunCoup" id="C5DBQ9">
        <property type="interactions" value="780"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBQ9"/>
    <dbReference type="GeneID" id="8290462"/>
    <dbReference type="KEGG" id="lth:KLTH0A04642g"/>
    <dbReference type="eggNOG" id="KOG1271">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044783_1_0_1"/>
    <dbReference type="InParanoid" id="C5DBQ9"/>
    <dbReference type="OMA" id="PTPSFQF"/>
    <dbReference type="OrthoDB" id="10069295at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000287">
        <property type="entry name" value="Protein-lysine N-methyltransferase EFM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03188">
        <property type="entry name" value="Methyltr_EFM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026635">
        <property type="entry name" value="Efm4/METTL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12843:SF5">
        <property type="entry name" value="EEF1A LYSINE METHYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12843">
        <property type="entry name" value="PROTEIN-LYSINE N-METHYLTRANSFERASE METTL10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13847">
        <property type="entry name" value="Methyltransf_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Methyltransferase" evidence="8">
        <location>
            <begin position="64"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR21216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="229" mass="25988" checksum="5ED33DA9FA9E8C51" modified="2009-07-28" version="1">MEDTSKLNTSKLGTKEYWDDFYALENSNFKENPGDTGECWFADNDAEEKMIEFLMDNLDQQNIHRDCSMIDLGTGNGHLLFQLHEEGFNGPMMGVDYSEKSVQFAREISESQFPGYENIKFEAADIFDPNWECSAYDVVLDKGTLDAIALSGIKLDGDRSVVDVYPKVVEKLLKKDSVFLITSCNFTQEELIRIVESDSLKVWKTIDYPVFEFGGVKGTTICSIAFVKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBR9</accession>
    <name>C5DBR9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0A04862g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551668.1">
        <property type="nucleotide sequence ID" value="XM_002551622.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBR9"/>
    <dbReference type="FunCoup" id="C5DBR9">
        <property type="interactions" value="126"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBR9"/>
    <dbReference type="GeneID" id="8290472"/>
    <dbReference type="KEGG" id="lth:KLTH0A04862g"/>
    <dbReference type="eggNOG" id="KOG0789">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007989_1_0_1"/>
    <dbReference type="InParanoid" id="C5DBR9"/>
    <dbReference type="OMA" id="KDWPDLG"/>
    <dbReference type="OrthoDB" id="6058203at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18533">
        <property type="entry name" value="PTP_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050348">
        <property type="entry name" value="Protein-Tyr_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134:SF547">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="6">
        <location>
            <begin position="145"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="4">
        <location>
            <begin position="537"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="5">
        <location>
            <begin position="735"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="80"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="284"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="83"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="314"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR21226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="871" mass="96188" checksum="D18DEDFBB2ABCB64" modified="2009-07-28" version="1">MESRWSSDQAELEAGCAAVSDASSVVSSSSTLVGEPGSKVAAFPPARPHRRAISALAIGRTMPGPAGALGPVGAIGSARPVGSPSSVNSAGSAGSQSPQTLPFLMRSNSNPSLPSFKAKEMRLPLPEGAQSMAAVELVSLLDSEPAEKLLVLDVRPFVDFCRATIQNALHVCLPSTLLRRKTFTLERLVDNLPEDVRQIVKPRVMDPEDCSDLKIVIFDNTAQQIDSGFSVGCHGMASKFVQSPKWATVEARPSVYILESGFAGFKEFAPDCVKAPVVTLSSPINSNSSPTDLSGAPNMMPKPKFSNHSVANGSPSSIPESPLSSSSPVSGLLKFQLPASNGLPPPFKIARNEEVLNLESYLSAVDLGEKQRSLGGSISSLENLDLSSPPSNTAFKFPTDPSSINQINLASGNGIVHADKLNFQKSFIQLHILYDRNMINSVVPRWFQDLAHEPKLEMISQFQKIDLLEKKRLSHCLSNACQPSRAQTASYAAANSCDTADLEHSNGFCPSRWLDEYDSDEEDQNICVSSGVELGTKNRYKDIFPYEHTRVVLKKTMPVYQHHYANEIFDTYINANYLTNPFVRLQTPPESDCLNVRYIATQAPLEATIHDFYTCILNNNVPLVLSLTDEFENGVEKCYKFWAEGNYDGIRIKLLQEHDLELPDVGPGVGPVTLRRIQIIYNENQTFETLQIQIKDWPDLGVMVNPRQILEMICLKNIVICQMFERRAYSHDHFPTILVHCSAGCGRTGTLCTLDTVLSNMSKFDSLKEEWIGRRDATEYNPWSPRSAACLNSKLFDPVIITINKFRKQRISMVQNVNQYLFIYDCLLSFFDMRLKEKAFLAHTSNPFRKTGKGLEIIESFLGSKTREAGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBU1</accession>
    <name>C5DBU1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0A05346p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0A05346g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551690.1">
        <property type="nucleotide sequence ID" value="XM_002551644.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBU1"/>
    <dbReference type="FunCoup" id="C5DBU1">
        <property type="interactions" value="230"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBU1"/>
    <dbReference type="GeneID" id="8290494"/>
    <dbReference type="KEGG" id="lth:KLTH0A05346g"/>
    <dbReference type="eggNOG" id="KOG1674">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018149_0_0_1"/>
    <dbReference type="InParanoid" id="C5DBU1"/>
    <dbReference type="OMA" id="SPMNKHW"/>
    <dbReference type="OrthoDB" id="10250320at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20557">
        <property type="entry name" value="CYCLIN_ScPCL1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013922">
        <property type="entry name" value="Cyclin_PHO80-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012104">
        <property type="entry name" value="PHO85_cyclin_1/2/9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615:SF10">
        <property type="entry name" value="PHO85 CYCLIN-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016511">
        <property type="entry name" value="Cyclin_Pcl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin N-terminal" evidence="2">
        <location>
            <begin position="44"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="209"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="278"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR21248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="301" mass="33370" checksum="DC7A2046686C59A9" modified="2009-07-28" version="1">MSDLEALLQFNKRRVSMEMIEFLASTSNSVIQVRTGESSAKVVSLVDFIKGLVVQSNVQTPTLMSTAVYLSKLRSIIPANVHGMETTRHRVFLGCLIIAAKNLNDSSPMNKHWASYTNGLLNTREVNTIERELLEYFDWNLRISTQDLVTCLSPFLTPIKDQLVSQKEDYLFFNSPVPSRSRLAAYHDSHSRSSSAMSIPSLVSSATMSTISTGGSRRTPLLPHSNSSNIHRIDENSYTYQSTKPVPGYAAPSLAKRSPSRPEKSHLARPIIMKTGLPGSTQSVSSQNKFGIKSNWASIFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBW9</accession>
    <name>C5DBW9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0A05962p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0A05962g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551718.1">
        <property type="nucleotide sequence ID" value="XM_002551672.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBW9"/>
    <dbReference type="FunCoup" id="C5DBW9">
        <property type="interactions" value="124"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBW9"/>
    <dbReference type="GeneID" id="8290522"/>
    <dbReference type="KEGG" id="lth:KLTH0A05962g"/>
    <dbReference type="eggNOG" id="KOG1281">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005170_8_0_1"/>
    <dbReference type="InParanoid" id="C5DBW9"/>
    <dbReference type="OMA" id="GYGLMYI"/>
    <dbReference type="OrthoDB" id="10260443at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006797">
        <property type="term" value="P:polyphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01115">
        <property type="entry name" value="SLC13_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14478">
        <property type="entry name" value="SPX_PHO87_PHO90_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004680">
        <property type="entry name" value="Cit_transptr-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10283:SF92">
        <property type="entry name" value="LOW-AFFINITY PHOSPHATE TRANSPORTER PHO91"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10283">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 13 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03600">
        <property type="entry name" value="CitMHS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03105">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="446"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="489"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="529"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="570"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="611"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="654"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="700"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="722"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="755"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="791"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="837"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="886"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="domain" description="SPX" evidence="8">
        <location>
            <begin position="64"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="191"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="194"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR21276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="907" mass="99847" checksum="3F0F18F311C73EED" modified="2009-07-28" version="1">MTASLYTSNLRHVAEVSRTCLRSRACPGGYSPVGDLPVHRQIASLIKVPRASHRSLALLPHRPMKFSHSLQFNAVPEWSSRYIAYSQLKRLIYTLQKEKLYQSSVSDPELVPLHDSYEHKFLEALDKELHKIDAFFAQQETRIFASYHELKDDVAEFYAQQPLHEFLSRRSSASGASAVSEGLRPALRHAASLSDDDDDDDDDDDDDDDDDGHNNRAGNGGHANSTNNSGFRRRGNSASEMVPLVSRSTHYAGLLDPLMEDRINLKKRLVVAYTQLSELKSFIELNQTGFSKICKKFDKSLDTNVKPGYIASLSSRAHVFSPAALADIDASIGDAISMYARLLGLSTDPNQDPAAIEEAERELSLHLREHVVWERNTVWKDMMNLERQAQNFKTAAHAKRKATQKSLDDEVRMSPSPDELPNATLTDALHMTPAHYFRVLLRSPGILRFTGITAIFLLILEFVPIRDTLQKNCLSIVIYASMLWATETIPLFVTSLLVPLLIVVLPVLKDSEGNVMTSQQSSQYILSTMWSSVIMLLLGGFTLAAALSKYNVAKVVSTHVLASAGTNPKIILLTNMCVALFVSMWVSNVAAPVLCYSIIQPLLRTLPKNSSFAKSLILGIALASNIGGMASPIASPQNIFSIGMMDPQPTWGQWFIVALPVCAICTLGIWVMLLISFPVDRSLKLLQLHPIKDPFTVQQWYVTLVTIVTIVLWCMAKQLSGIFGEMGIISIIPILLLFGTGMLSSSDFNNFMWTIVVLAMGGTTLGKAVTSSGLLASIANTIKDHVQDKPIFLIVLIFGIVILVMATFVSHTVAAMIIVPLMSEIGKNLPNGDHSRLLIMIAALLCSAAMGLPTSGFPNVTAISMIDELGDRYLTVGNFITRGVPASIWSYFVITTVGYGLMKLIGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DC19</accession>
    <name>C5DC19_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0A07128p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0A07128g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551768.1">
        <property type="nucleotide sequence ID" value="XM_002551722.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DC19"/>
    <dbReference type="FunCoup" id="C5DC19">
        <property type="interactions" value="35"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DC19"/>
    <dbReference type="GeneID" id="8290575"/>
    <dbReference type="KEGG" id="lth:KLTH0A07128g"/>
    <dbReference type="eggNOG" id="ENOG502QV77">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_423382_0_0_1"/>
    <dbReference type="InParanoid" id="C5DC19"/>
    <dbReference type="OMA" id="FEVWSTG"/>
    <dbReference type="OrthoDB" id="5382797at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="206"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="237"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="279"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="343"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="375"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="398"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR21326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="566" mass="62897" checksum="C1EF7289F79BA6C7" modified="2009-07-28" version="1">MGSPNLSTKEDFGSSFSFGGLPSTPQHMNCIVEEDHAKDGLAEEMEREPSKTPQRPLSFYSNADGNNSSNSLIMNPAFSFGASATQENKRHSMKYVPTPKLPPSSSSRTKSPARGNRSPSPDRGKRRGSLVLDKPFNFSSSTLQPPASAGSATRASFRKGHRYKHSSVSMNFFQEPEVQVPLNIAKAMPIPDFADLKSNIPWPHGYAQLAIAALQIIACLVTFRLGHVKSWNNFITLSHFVLYDVIGSLAIILVENLSQFQVWNTGTITFPFGLNRMDVLLSFALAISLCFMGLDLFFHILEETIVLFVESTNDSNQHNDIAPKIPHSHHSSAFLHTASETQIWYWTLLPNLALSLLSLFKTYHSNAHTKFKTKNPVITCTYILYLSLYPLIQKVVSSSDYIATSLLSIFIMSYGWTIAKWTSTILLMGFSTASLNGLILDDDPSLSDDRPTKALLVRSKSTLPIAVANNNSSKPNKNNSKICDSTHVKSKIMESVQELPLFRANCKLANEDLVIVKVNFDQYIVLIRLRMAGGSNDDELNLRLAIDKCIRKILKTVDTTIDIDRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>C5DC29</accession>
    <name>C5DC29_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0A07348p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0A07348g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551778.1">
        <property type="nucleotide sequence ID" value="XM_002551732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DC29"/>
    <dbReference type="FunCoup" id="C5DC29">
        <property type="interactions" value="609"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DC29"/>
    <dbReference type="GeneID" id="8290585"/>
    <dbReference type="KEGG" id="lth:KLTH0A07348g"/>
    <dbReference type="eggNOG" id="KOG3655">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_459326_0_0_1"/>
    <dbReference type="InParanoid" id="C5DC29"/>
    <dbReference type="OMA" id="NSARNAW"/>
    <dbReference type="OrthoDB" id="5971719at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030864">
        <property type="term" value="C:cortical actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030427">
        <property type="term" value="C:site of polarized growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11281">
        <property type="entry name" value="ADF_drebrin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11961">
        <property type="entry name" value="SH3_Abp1_fungi_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000052">
        <property type="entry name" value="Actin-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035718">
        <property type="entry name" value="Abp1_fungi_SH3_C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002108">
        <property type="entry name" value="ADF-H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10829">
        <property type="entry name" value="CORTACTIN AND DREBRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10829:SF25">
        <property type="entry name" value="DREBRIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00241">
        <property type="entry name" value="Cofilin_ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00102">
        <property type="entry name" value="ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51263">
        <property type="entry name" value="ADF_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="ADF-H" evidence="7">
        <location>
            <begin position="7"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="6">
        <location>
            <begin position="566"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="142"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="151"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="187"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="224"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="263"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="291"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="369"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="445"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="470"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="491"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="539"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR21336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="626" mass="68158" checksum="E644C0EA06AB306F" modified="2009-07-28" version="1">MALEPIDYTTHCREIEEVFQKVVRGSDAEIEWLVLSPNSKKEYTAESVGSGFSEFLGTFEDTKVQFGLARVSPPGSDVQKLILIGWCPDSAPLKTRASFASNFGVVANQVLKSYHVQVTARDEDDLDENELLMKISNAAGARYSIQQEPTRTSSAPKPRPAATPKATPSSNNVSTAPKPSADFTPKPSTTASVPKAAPASAPKPAAQFSTPVSGRDAGRSNSSGEDDDWDEPEIEERDFDSKPLKPNKSSWQPVGKVDLQKLIAEENAKQDPRLVNMVKSDTPVKLNPQDDIQKLKQESRAQRDAELNKFMGTKPPTGSVPKKDDDKVIRGFQNEKSPAQLWAEKHQQKVTSPQKASAATEAAEGKKEDEDDEEEEAPEVSNVKAKFEKMAVNEPTIIKPGQPKTPAPVESDSAPAPPKASLGRPLPGMHTELDDDQDANSSNAQDDDDDWDDQSDEEVKPPAPAPRPSLPARTSTATSSSPAPAPGPRPQSNLTQEVPTKTQVDEKPAAAAAPSLPTRTSESSYVPPPPPRRNVPVTEEPEEPEEPETVEGSQKNAAEDELQLEEKLPSAIAEYDNEAEEHNELAFEEGDKIINISFVDDDWWLGELEKTGEKGLFPSNYVQLIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>C5DC80</accession>
    <name>C5DC80_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">KLTH0B00858p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0B00858g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551829.1">
        <property type="nucleotide sequence ID" value="XM_002551783.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DC80"/>
    <dbReference type="FunCoup" id="C5DC80">
        <property type="interactions" value="823"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DC80"/>
    <dbReference type="GeneID" id="8290655"/>
    <dbReference type="KEGG" id="lth:KLTH0B00858g"/>
    <dbReference type="eggNOG" id="KOG0767">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039456_1_0_1"/>
    <dbReference type="InParanoid" id="C5DC80"/>
    <dbReference type="OMA" id="KMYIEEG"/>
    <dbReference type="OrthoDB" id="427452at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990547">
        <property type="term" value="P:mitochondrial phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000024">
        <property type="entry name" value="MIR1p Mitochondrial phosphate carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044677">
        <property type="entry name" value="SLC25A3/Pic2/Mir1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45671:SF12">
        <property type="entry name" value="MITOCHONDRIAL PHOSPHATE CARRIER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45671">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25 (MITOCHONDRIAL CARRIER PHOSPHATE CARRIER), MEMBER 3, LIKE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="11"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="110"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="208"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR21391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="305" mass="32189" checksum="81F6346EF31CB2B9" modified="2009-07-28" version="1">MSAPIPQYSMSDYAKFALAGAIGCGVTHSAMVPIDVVKTRIQLEPTVYNKGMVSSFKQIVSAEGAGALLTGFGPTLLGYSMQGAFKFGGYEVFKKLFIDTLGYDQAVNYKNSIYIGSAAIAEFFADIALCPLEATRIRLVSQPSFADGLFGGFSRILREEGAASFYNGFTPILFKQIPYNIAKFLVYERAAEVYFGMVGPKDTLSNTTATGLNLLSGLTAGLAAAVVSQPADTLLSKVNKAKKAPGQSTIGLLGQLAKELGFVGSFAGLPTRLVMVGTLTSLQFGIYGTLKKSLGCAPAIEIASK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCF8</accession>
    <name>C5DCF8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AP complex subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0B02706g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Adaptins are components of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the adaptor complexes large subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551907.1">
        <property type="nucleotide sequence ID" value="XM_002551861.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCF8"/>
    <dbReference type="FunCoup" id="C5DCF8">
        <property type="interactions" value="126"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCF8"/>
    <dbReference type="GeneID" id="8290738"/>
    <dbReference type="KEGG" id="lth:KLTH0B02706g"/>
    <dbReference type="eggNOG" id="KOG1061">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006320_4_4_1"/>
    <dbReference type="InParanoid" id="C5DCF8"/>
    <dbReference type="OMA" id="RIFARYK"/>
    <dbReference type="OrthoDB" id="10254310at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030117">
        <property type="term" value="C:membrane coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026739">
        <property type="entry name" value="AP_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016342">
        <property type="entry name" value="AP_complex_bsu_1_2_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11134">
        <property type="entry name" value="ADAPTOR COMPLEX SUBUNIT BETA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002291">
        <property type="entry name" value="AP_complex_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Clathrin/coatomer adaptor adaptin-like N-terminal" evidence="8">
        <location>
            <begin position="18"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="629"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="630"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="651"/>
            <end position="660"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR002291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR21469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="697" mass="79127" checksum="FD12B6FFCF83CE04" modified="2009-07-28" version="1">MSELRLFSKCKAYEIRAELQQTNIKKHRASATKRKDALRKIIANLSMGNFGEMALLFPDVLRFWQVEDDLEVKRICHHYLVAMAPTKSGHFATALSMVLEDFNSGTEAVQILALRTLSSVPLPAYLEEASKCASAVLHQHSEPEALKKAALYALLRLAQLDSERSQLLMGIVGKVLQSAKEKPSVRAHALFVLYQEEESSSETRTMGLGRDLCLSMLELLPRLNEWDNGRVLDALTANYVPQTHDDAHFMIDKALPQLQHANTSVVLNALKLIVFLTNYVDRLSESIVKQLSSSVISLLNKPPELQFLVLRNVILLLLGREKPLLKVDVPYFFVEFNDPIYIKDTKLEILYLLAKEDNLAQIFQELKEYATDIDIQMSRKAIRAVGNLAVKLKNSVDECINLLLDLLDFEVEYIVQEIISVFKNVLRRYPEKSKLCLYKLVRFTDSVQEPESRSSMIWIITQYSSQLPNYFELFEGFSNNFLEESLEVQFTILSSTVKLFTRHPTPATEKLCINILKSSTEKLDNPDLRDRAFMYWRLLSCTQKSGGNILTMDAVREVVDGNLPLITLNSRLDPHVIEELELSIGTIVSIYLKPVNQVFRLNKPRVLPKSPALNSDKDNLEIIADDTRSHSSSFTDDSSLASRRQSSSPVKKMDDYDRPAEVVNQLKLKRKTSSATSLLSRKPSVLGRKLSMKRPFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCL8</accession>
    <name>C5DCL8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0B04114p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0B04114g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CLPTM1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551967.1">
        <property type="nucleotide sequence ID" value="XM_002551921.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCL8"/>
    <dbReference type="STRING" id="559295.C5DCL8"/>
    <dbReference type="GeneID" id="8290802"/>
    <dbReference type="KEGG" id="lth:KLTH0B04114g"/>
    <dbReference type="eggNOG" id="KOG2489">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019907_2_0_1"/>
    <dbReference type="InParanoid" id="C5DCL8"/>
    <dbReference type="OMA" id="DAYPIND"/>
    <dbReference type="OrthoDB" id="378564at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008429">
        <property type="entry name" value="CLPTM1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21347">
        <property type="entry name" value="CLEFT LIP AND PALATE ASSOCIATED TRANSMEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21347:SF0">
        <property type="entry name" value="LIPID SCRAMBLASE CLPTM1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05602">
        <property type="entry name" value="CLPTM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="416"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="447"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="477"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="555"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="580"/>
            <end position="608"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR21529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="691" mass="78119" checksum="B865941F0247A5F8" modified="2009-07-28" version="1">MNAAQPSEGRSYIQYGVLALSLASLLKKSLSPSKEPINALNCAQDEEKNITSTTFSQRVARRIISSALEKRSLKVGDLKKSQERLEYESSLKVLPQENETFVEHTPPSVQSINITSTSHANGIPEYFLEEAPTLEALAYKNSKKAPKLLIPGWRAGTPLALEYYLSTNSSYDRADFSPQDSSAWRGSLDSLDLDEPQTISENFKFRVPEGFGNDFVYLHLYLMTSCEGSEEVGYTNCEYVSKNTIPLSYKLTKTEKAAKKNLLASKKKTREDNIGYELSYMDDVPFWHPEITIYLGKPFGIVEADSVPKAMKSLISVDEGTKRDNLTGLAQRYSPPVLYNNFWQMAHEMIEINTTTRELPINLRLEIPPAWKFRVYSTALHNLRELAMFGGASKANSSVFTLDHYKLVWLGTKTSVLALTVIATVLHSALGVLALKSDTAHWQKRENHIGVSVRGLFANILMQALVMLYLLDNFEGTSSTVFVSQVVGFLIECWKVSTILKIQIVASREEASLNADTEVVMMPLARGKRLAISNKHLLNEEEKKTREYDATAFRILLKVFVPLILGYAGFSLVYGEHKSWYSFFVRVMVGIVYTYGFLIQLPAVYINYKLKSVAHMSKRAFAYKVVNTFIDDLFAFVAKMPWLHKIATFRDDVVFFIYLYQFWAYRVDYARTNEFGEPAAAPGSEKAEKQE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-03-08" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCN9</accession>
    <name>AEP1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName>ATPase expression protein 1, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">AEP1</name>
        <name type="ordered locus">KLTH0B04620g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for translation of the mitochondrial OLI1 transcript encoding subunit 9 of mitochondrial ATP synthase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the AEP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551988.1">
        <property type="nucleotide sequence ID" value="XM_002551942.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCN9"/>
    <dbReference type="FunCoup" id="C5DCN9">
        <property type="interactions" value="173"/>
    </dbReference>
    <dbReference type="GeneID" id="8290825"/>
    <dbReference type="KEGG" id="lth:KLTH0B04620g"/>
    <dbReference type="eggNOG" id="ENOG502RXUX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035453_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCN9"/>
    <dbReference type="OMA" id="CKVEANE"/>
    <dbReference type="OrthoDB" id="4064791at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045182">
        <property type="term" value="F:translation regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031467">
        <property type="entry name" value="Aep1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17049">
        <property type="entry name" value="AEP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0496">Mitochondrion</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0809">Transit peptide</keyword>
    <keyword id="KW-0810">Translation regulation</keyword>
    <feature type="transit peptide" description="Mitochondrion" evidence="2">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000405604" description="ATPase expression protein 1, mitochondrial">
        <location>
            <begin position="14"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="566" mass="63976" checksum="5987C303CF2CF746" modified="2009-07-28" version="1" precursor="true">MGSRIFSPRRLYSALSELKTETVIPKNPNKRTRDGSFRKFKRVAPAIGDSMHPFYSPNIMERAILCASEIKPELLDGQPIVPAVIKQLKTLEPALVNTRWQPQSTQGLNDWLEPFRKMRRKSSPLKLIENHEIDNVIRPSRIDSGRIPELRKFALMFEKEDAGILSASTIGSLIDRLAADQEKAVFSEEVFLYILQHYCKSSQGIASVVDSITEFLHKDIDDLKTAETLLAHVLMALRRNSIPLTPRATSAILKLIDSVSTRFHRPFCVVDFSPAVVQMTTEFYVDSGFLKESKVLFTDMVNKERCPSAQLVEKYLGLIESVCGISTSDNDFLKKFVYISNFRPIFQTTMTPRITEFLVSYCRHFDEILSLLVLVDHSKVKKQIWDLVLPQMIRRVSLLTKDSAKNCCHLTVLYQKASRFYGMPLSTKVNKAFIIQYAVNGNFAMVARLLSIIDSNAFPSFYASVLAAYDQSSAFSMEVPSSGAALKNKHQFMTSMIIPHYTEISFVGRQLALKHADTEELLEQVLKAEIAIKGRGGKSLLPQVSLKAQDCKSNYIITEAEKCLQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCZ2</accession>
    <name>C5DCZ2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0B06996p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0B06996g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552091.1">
        <property type="nucleotide sequence ID" value="XM_002552045.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCZ2"/>
    <dbReference type="FunCoup" id="C5DCZ2">
        <property type="interactions" value="223"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCZ2"/>
    <dbReference type="GeneID" id="8290931"/>
    <dbReference type="KEGG" id="lth:KLTH0B06996g"/>
    <dbReference type="eggNOG" id="KOG0393">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041217_21_1_1"/>
    <dbReference type="InParanoid" id="C5DCZ2"/>
    <dbReference type="OMA" id="MRSVKCV"/>
    <dbReference type="OrthoDB" id="8830751at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00157">
        <property type="entry name" value="Rho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001179">
        <property type="entry name" value="Rho family GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="205"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="215"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="246"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR21653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="259" mass="28941" checksum="729A8532F88359A3" modified="2009-07-28" version="1">MRSVKCVIVGDGAVGKTSLLISYTTNTFPQDYIPTVFDNYTTTIALNDGKSIEPQVFRLNLWDTAGQEEYDRLRPLSYPQTDIFLLCFSINEPNSFMNVKDKWFPEIRHNTNYDNLELFRVCGKYPILLVGTKADLRDDEHEQDRLAEVNSDFVSRAEIDQCISQCGFMGYVECSAATQVGVAEVFERAVQCVIIEPERLLNQSKQRESTIDHPTNNQSNTAASSGAGAGTAGSSSNWNAPGSNPMKKRKKKSQKCTIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>C5DD17</accession>
    <name>C5DD17_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0B07568p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0B07568g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Bud neck</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552116.1">
        <property type="nucleotide sequence ID" value="XM_002552070.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DD17"/>
    <dbReference type="FunCoup" id="C5DD17">
        <property type="interactions" value="219"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DD17"/>
    <dbReference type="GeneID" id="8290956"/>
    <dbReference type="KEGG" id="lth:KLTH0B07568g"/>
    <dbReference type="eggNOG" id="KOG2655">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017718_7_4_1"/>
    <dbReference type="InParanoid" id="C5DD17"/>
    <dbReference type="OMA" id="IQECKFL"/>
    <dbReference type="OrthoDB" id="416553at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031105">
        <property type="term" value="C:septin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01850">
        <property type="entry name" value="CDC_Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016491">
        <property type="entry name" value="Septin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025662">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006698">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00675">
        <property type="entry name" value="SIGMA54_INTERACT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="6">
        <location>
            <begin position="10"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="102"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="376"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="424"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="501"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="109"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="376"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="426"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="502"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR21678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="544" mass="61661" checksum="8C48BD3476CEE22A" modified="2009-07-28" version="1">MYRRKKEHKRGINYSLMLVGDSGTGKTTFANNLLESPIFPHRFAMDAPQLAFNPRVQIAKPTMVVSYNSKNGIPSHMAQFDASREHFEPGITITSTSVEIATGAEQPPSRSSMSSEASSSAQSNSHSQDKISFNLVKTHGLGENLDNTMCFDEITAFLEQQFDQVLAEETRVKRNPRFEDTRVHVTLYFIPPTGHGLREMDVEMIKRLSRYTNVLPIISRADSFTPEELIKFKRQIMDDVERFHVPVYKFEVDPEEDDIETIEENQALAMMQPFAIMCSDEKNENGEYVRLYPWGEMRVDDDSISDLRVLKNVLFGSHFQEFKDTTQNFLYENYRAEKLSSVSEWDMDKEKGESSKRDSAAPSLSNFASLITTGQFKSTNSLAMPPQSETPTTPDSKEISQGVSDSPALRQMSQSIRRGNEEIIQNIKNSPRTSTPGSPDKSKLRNISETVPYVLKHERIIAKQQKLEELEAQSAKELQKRIQDLEKRAAELKLREKLLRQQHLNSSNTSLNSRQSAQQSMAPQSKLKKEESVTDLASIVSGKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>C5DD94</accession>
    <name>C5DD94_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0B09350p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0B09350g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552193.1">
        <property type="nucleotide sequence ID" value="XM_002552147.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DD94"/>
    <dbReference type="FunCoup" id="C5DD94">
        <property type="interactions" value="29"/>
    </dbReference>
    <dbReference type="GeneID" id="8291036"/>
    <dbReference type="KEGG" id="lth:KLTH0B09350g"/>
    <dbReference type="eggNOG" id="ENOG502S1YW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1759093_0_0_1"/>
    <dbReference type="InParanoid" id="C5DD94"/>
    <dbReference type="OMA" id="STRINYE"/>
    <dbReference type="OrthoDB" id="3979149at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR21755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="140" mass="15991" checksum="96FB7D14B3525262" modified="2009-07-28" version="1">MAQRTPQIYKLFQYVCAGLALIAAVEYFKYSTRINYEWFHCTLVEEPWEQGSSLSRLYAVGGPSCDKRGELKTIMKKITRGFDLQKEGISFCIKEDLEVPSVHYPIHDKKGAPGYVAYASYDSDSRDIREVCEDAPVLHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDR9</accession>
    <name>C5DDR9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0C03278p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0C03278g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the glycosyl hydrolase 32 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552368.1">
        <property type="nucleotide sequence ID" value="XM_002552322.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDR9"/>
    <dbReference type="FunCoup" id="C5DDR9">
        <property type="interactions" value="488"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDR9"/>
    <dbReference type="GeneID" id="8291232"/>
    <dbReference type="KEGG" id="lth:KLTH0C03278g"/>
    <dbReference type="eggNOG" id="KOG0228">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001528_3_3_1"/>
    <dbReference type="InParanoid" id="C5DDR9"/>
    <dbReference type="OMA" id="GTEWRHA"/>
    <dbReference type="OrthoDB" id="202537at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004575">
        <property type="term" value="F:sucrose alpha-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005987">
        <property type="term" value="P:sucrose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18622">
        <property type="entry name" value="GH32_Inu-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.115.10.20:FF:000002">
        <property type="entry name" value="Invertase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.560">
        <property type="entry name" value="Exo-inulinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.115.10.20">
        <property type="entry name" value="Glycosyl hydrolase domain, family 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001362">
        <property type="entry name" value="Glyco_hydro_32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018053">
        <property type="entry name" value="Glyco_hydro_32_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013189">
        <property type="entry name" value="Glyco_hydro_32_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013148">
        <property type="entry name" value="Glyco_hydro_32_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023296">
        <property type="entry name" value="Glyco_hydro_beta-prop_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42800">
        <property type="entry name" value="EXOINULINASE INUD (AFU_ORTHOLOGUE AFUA_5G00480)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42800:SF4">
        <property type="entry name" value="INVERTASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08244">
        <property type="entry name" value="Glyco_hydro_32C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00251">
        <property type="entry name" value="Glyco_hydro_32N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00640">
        <property type="entry name" value="Glyco_32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75005">
        <property type="entry name" value="Arabinanase/levansucrase/invertase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00609">
        <property type="entry name" value="GLYCOSYL_HYDROL_F32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 32 N-terminal" evidence="8">
        <location>
            <begin position="79"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 32 C-terminal" evidence="9">
        <location>
            <begin position="445"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="50"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="16"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="50"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR21930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="599" mass="66727" checksum="F17874238EADB9A3" modified="2009-07-28" version="1">MAFSSLKKSDSVGSTPHRKQNHPNKRKPFSKMQLLGSALGALVIAAGVTASQSNSRSRGKNTDSQPQFNASSGTRPLVHLTPNKGWMNDPNGLFYDSEASIWHAYYQYNPNDNVWGLPLYWGHATSEDLTVWEDHGAAIAPQNDESGAFSGSIIIDANNTSGFFNDSTKPAQRIVAFYTEHNATTESQYAAYSLDGGFTFEQYEHNPVLDIGSDQFRDPKVFWHEESERWVMTLALSQEYTIQIFTSENLKDWELQSNFSHHGILGYQYECPGLAKVRVERDIPLNVTSNGKTNLTTQDDGAEYKWVMFLSINPGSPAGGSVNEYFIGDFDGKTFTPQDSAARIVDHGKDFYALQTFTNAPNGDTIGIAWASNWDYCAFVPTEAWKSSMSIARNFTVRPYNPNPQSTELNLYSTPILNSTDLQTKRYLKKSSLEIGADEALKYNLGNDAQGLLEFTATWKVNGSSIPSVGDFADLSFWFTGKEDDDEYLRLGYAVNAAGFFVDRGNTDVDFVNDNPFFNNRQTQNIETYKIGTDGLPIYKVHGIIDRNIIELFFNDGSSTSTNLFFLSDGNYIGGVEITAGVEGVFTILDFEIKQLARK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDS1</accession>
    <name>C5DDS1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0C03322p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0C03322g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552370.1">
        <property type="nucleotide sequence ID" value="XM_002552324.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDS1"/>
    <dbReference type="FunCoup" id="C5DDS1">
        <property type="interactions" value="557"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDS1"/>
    <dbReference type="GeneID" id="8291234"/>
    <dbReference type="KEGG" id="lth:KLTH0C03322g"/>
    <dbReference type="eggNOG" id="KOG2178">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008831_1_2_1"/>
    <dbReference type="InParanoid" id="C5DDS1"/>
    <dbReference type="OMA" id="MRMRLCC"/>
    <dbReference type="OrthoDB" id="24581at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003951">
        <property type="term" value="F:NAD+ kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019674">
        <property type="term" value="P:NAD+ metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006741">
        <property type="term" value="P:NADP+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.30:FF:000005">
        <property type="entry name" value="NAD+ kinase Utr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.30">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00361">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017437">
        <property type="entry name" value="ATP-NAD_kinase_PpnK-typ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002504">
        <property type="entry name" value="NADK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275">
        <property type="entry name" value="NAD KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275:SF0">
        <property type="entry name" value="NAD KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01513">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20143">
        <property type="entry name" value="NAD_kinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="475"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="481"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="497"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="508"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR21932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="532" mass="59624" checksum="8BBB50D9D17DADE1" modified="2009-07-28" version="1">MARHGDAPFSVPLSRELSPLSRTLTDGGEKKSHGTNSKGKGEITNKSKRSKSVSHLADRNKILGSRMTPIKPEEQKVPEEPLEDPLEEPHRVDTAEKVFRRLSVGSNDMRRATSHAQLSSTAHGMRLLSKDIQKSMVVLQVNRLMIVTKKQDSSLVYLTREMVEWILVNYPEIEIYVDETIECSNRFDTKGIIRDSRCGSNRIKTWSPELVAKKDDFFDLVITLGGDGTVLYVSSLFQRSIPPVMSFSLGSLGFLTNFNYENFRQSLPRVLNSKIRSKMRMRLCCRVFRKRKPNKENNNSRSRKKFTMIGEYHVLNELTIDRGPSAFISMLEVFGDNSLLTVAQADGLIIATPTGSTAYSLSAGGSLVYPSVNAIAVTPICPHTLSFRPIILPDSMKLKVKVPLNSRATAWAAFDGKNRVELFKGDYVCITASPHSFPTLESSPTEFIDSISRTLNWNAREPQKSFAHMLSQKNQKNYVSDTEKQKQDQPEVRAPSDEEEEEGESDSESPSQFRPKAASSAASPKLKPNFQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDU3</accession>
    <name>C5DDU3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0C03828p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0C03828g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ANP1/MMN9/VAN1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552392.1">
        <property type="nucleotide sequence ID" value="XM_002552346.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDU3"/>
    <dbReference type="FunCoup" id="C5DDU3">
        <property type="interactions" value="78"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDU3"/>
    <dbReference type="GeneID" id="8291256"/>
    <dbReference type="KEGG" id="lth:KLTH0C03828g"/>
    <dbReference type="eggNOG" id="ENOG502QRNF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017872_1_1_1"/>
    <dbReference type="InParanoid" id="C5DDU3"/>
    <dbReference type="OMA" id="YAEYPTW"/>
    <dbReference type="OrthoDB" id="204164at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000136">
        <property type="term" value="C:mannan polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000009">
        <property type="term" value="F:alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000032">
        <property type="term" value="P:cell wall mannoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000163">
        <property type="entry name" value="Van1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052086">
        <property type="entry name" value="Mannan_Polymerase_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43083:SF5">
        <property type="entry name" value="MANNAN POLYMERASE I COMPLEX VAN1 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43083">
        <property type="entry name" value="MANNAN POLYMERASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03452">
        <property type="entry name" value="Anp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00037964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="404" mass="46512" checksum="173A131E7B261FD4" modified="2009-07-28" version="1">MLRKFDNGAEHYLISKGGQEILDSSDKERYEEEVKSLLDSTVEHYDLRDFEGSAQGEVNREHVLLLVPLRNAEQVLPLMFKNLMNLTYPHELIDLAFLVSDCSEDDKTLEALLEYCKELQSGTLSDVFAAHSSDEELIKEKGTHDLFLKYMRPSYLKMVKDAYSPPFHKGYDKPFRSVQIFQKDFGQIIGQGFSDRHAVKVQGIRRKLMGRARNWLTANALKPYHSWVYWRDADVELCPGSVIQDLMSTDFDVAVPNVWRPLPEFLQGEQPYDLNSWIESDPALQLAQTLDEDDVIVEGYAEYPTWRVHLAYLRDPDSNPKDIIELDGVGGVSILAKAKLFRNGVHFPAFTFENHAETEAFGKMAKKMGYRVAGLPHYTLWHIYEPSDDDLIQIAEREREKKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDV6</accession>
    <name>C5DDV6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0C04136p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0C04136g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552405.1">
        <property type="nucleotide sequence ID" value="XM_002552359.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDV6"/>
    <dbReference type="FunCoup" id="C5DDV6">
        <property type="interactions" value="40"/>
    </dbReference>
    <dbReference type="GeneID" id="8291270"/>
    <dbReference type="KEGG" id="lth:KLTH0C04136g"/>
    <dbReference type="eggNOG" id="ENOG502S4N7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_831968_0_0_1"/>
    <dbReference type="InParanoid" id="C5DDV6"/>
    <dbReference type="OMA" id="CHAIMTI"/>
    <dbReference type="OrthoDB" id="4068218at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012935">
        <property type="entry name" value="NuBaID_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07967">
        <property type="entry name" value="zf-C3HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C3HC-type" evidence="3">
        <location>
            <begin position="98"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR21967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="312" mass="35447" checksum="8D8CC16A87F26D4B" modified="2009-07-28" version="1">MDDALLARFEAAKRKIDAPAVPWQALRPRNRASHIVNKYKYASRAHGVERNAYLTENELQGRRLYEKRSAGASGLSSRQEFMQAVRELVQVTRHSTVLWNSQRVNVVQMASKGWHVHKVQKGGSHGAALHCKNCRGSFYLELDDYLESEQLEARFNALLTERHVPGCYWRSKTYNLAHNYYLTDVSLYLEFERINKELGKYKDSGVMPDVPHNDDTIALARTFGASGVSLGPLALALRGYTSIDSEIAECTGCFRRTFVATLREPNANNHASWCKFADEQLLIKMLLKHLKPQKNSSIGDRLQDLESRLHGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDY7</accession>
    <name>C5DDY7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0C04862p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0C04862g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552436.1">
        <property type="nucleotide sequence ID" value="XM_002552390.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDY7"/>
    <dbReference type="FunCoup" id="C5DDY7">
        <property type="interactions" value="229"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDY7"/>
    <dbReference type="GeneID" id="8291303"/>
    <dbReference type="KEGG" id="lth:KLTH0C04862g"/>
    <dbReference type="eggNOG" id="KOG4139">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023948_0_0_1"/>
    <dbReference type="InParanoid" id="C5DDY7"/>
    <dbReference type="OMA" id="GMKIWAI"/>
    <dbReference type="OrthoDB" id="21380at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031431">
        <property type="term" value="C:Dbf4-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043539">
        <property type="term" value="F:protein serine/threonine kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010571">
        <property type="term" value="P:positive regulation of nuclear cell cycle DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901987">
        <property type="term" value="P:regulation of cell cycle phase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.250.3410:FF:000001">
        <property type="entry name" value="Protein DBF4 homolog A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3410">
        <property type="entry name" value="DBF zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055116">
        <property type="entry name" value="DBF4_BRCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013939">
        <property type="entry name" value="Regulatory_Dfp1/Him1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051590">
        <property type="entry name" value="Replication_Regulatory_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006572">
        <property type="entry name" value="Znf_DBF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038545">
        <property type="entry name" value="Znf_DBF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15375">
        <property type="entry name" value="ACTIVATOR OF S-PHASE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15375:SF26">
        <property type="entry name" value="PROTEIN CHIFFON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22437">
        <property type="entry name" value="DBF4_BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08630">
        <property type="entry name" value="Dfp1_Him1_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07535">
        <property type="entry name" value="zf-DBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00586">
        <property type="entry name" value="ZnF_DBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51265">
        <property type="entry name" value="ZF_DBF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DBF4-type" evidence="6">
        <location>
            <begin position="510"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="351"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="415"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="466"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="487"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR21998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="566" mass="64048" checksum="707B79D7BE72063B" modified="2009-07-28" version="1">MMSPSKGSMATRRSPLKETNANLKTGTDMVKRRAVEKLELQHRKRAKTAETGRRTIEGAVQTQRQAEFKKVGESAKVTPRDLLEWQTNWKRIMRRDTKIYFDTTEVADAKFKPDKRKELMRRGFVSLGAEITPFFDTDVTIVITQRAVKDCDRLPETDVLAKASKRYMKVWNYDKAVRFLKNLDVDLDALDTKAPAAPSTLSNLLQNEKLYGPSDRDPKARRDDVHYFKYPHVYLYDLWQVWAPIITAEWRPQDFANPDRLPYPVVKPGTFGRCPFIGDGHCDESSAKRIIKRYKRDALNERYALHLRVLYQHSAEPRNLVFKEEDSRPLILPNSFPNSYKSYRGLLSANQPAEEAKNNRSPEPVSAKSTTSVTLKPPSKAIWRAPQLNQPALLLSRQDTEELADDLCRKKPRIPQEIKASGVNPSNDGAAGAGNGLAPTKASVMNKNMKSLNRLVVDKKFANTSTGSAAEPHAAAGPKPACASPRTGDDADKQKQKELKQQQDLRRNDTRLGSGYCENCRVKYECLAHHINSEKHQHFAHDDINFEAIDSLIEKLAASRALSCSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DE21</accession>
    <name>C5DE21_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Protein YOP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0C05632g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required to generate and maintain the structure of the tubular endoplasmic reticulum network and the vacuole. Induces high curvature in membranes and causes membrane tubule formation. Involved in membrane/vesicle trafficking.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the DP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552470.1">
        <property type="nucleotide sequence ID" value="XM_002552424.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DE21"/>
    <dbReference type="FunCoup" id="C5DE21">
        <property type="interactions" value="387"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DE21"/>
    <dbReference type="GeneID" id="8291337"/>
    <dbReference type="KEGG" id="lth:KLTH0C05632g"/>
    <dbReference type="eggNOG" id="KOG1725">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028431_2_1_1"/>
    <dbReference type="InParanoid" id="C5DE21"/>
    <dbReference type="OMA" id="DTQYWVV"/>
    <dbReference type="OrthoDB" id="10009287at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004345">
        <property type="entry name" value="TB2_DP1_HVA22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12300">
        <property type="entry name" value="HVA22-LIKE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12300:SF161">
        <property type="entry name" value="RECEPTOR EXPRESSION-ENHANCING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03134">
        <property type="entry name" value="TB2_DP1_HVA22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="38"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="112"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="163"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="169"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00045873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR22032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="183" mass="20128" checksum="5A644C14504B3534" modified="2009-07-28" version="1">MGDYVKAFQDELNTLDKKFAGNGVLEQFEAKTKLPKSYGVLGAASLYLLLIFINVGGIGEILSNFIGFVIPTYYSLVALKTPGGADDTELLTYWVVFAFLNVIEFWSKAILYWVPFYWFMKTLLLVYLALPQTSGAKYVYNAVLEPVTSKYIKVPQAQAGGVSSKMEGLSTSGKTTGFASHSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DE58</accession>
    <name>C5DE58_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="15">KLTH0C06468p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0C06468g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Nucleoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the non-repetitive/WGA-negative nucleoporin family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552507.1">
        <property type="nucleotide sequence ID" value="XM_002552461.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DE58">
        <property type="interactions" value="1266"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DE58"/>
    <dbReference type="GeneID" id="8291375"/>
    <dbReference type="KEGG" id="lth:KLTH0C06468g"/>
    <dbReference type="eggNOG" id="KOG1900">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000429_0_1_1"/>
    <dbReference type="InParanoid" id="C5DE58"/>
    <dbReference type="OMA" id="SWAPFQK"/>
    <dbReference type="OrthoDB" id="338970at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044611">
        <property type="term" value="C:nuclear pore inner ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051292">
        <property type="term" value="P:nuclear pore complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036228">
        <property type="term" value="P:protein localization to nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006405">
        <property type="term" value="P:RNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000972">
        <property type="term" value="P:transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.440:FF:000001">
        <property type="entry name" value="Nuclear pore complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.450:FF:000002">
        <property type="entry name" value="Putative non-repetitive nucleoporin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.440">
        <property type="entry name" value="Nucleoporin, helical domain, central subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.450">
        <property type="entry name" value="Nucleoporin, helical domain, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007187">
        <property type="entry name" value="Nucleoporin_Nup133/Nup155_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014908">
        <property type="entry name" value="Nucleoporin_Nup133/Nup155_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004870">
        <property type="entry name" value="Nucleoporin_Nup155"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042533">
        <property type="entry name" value="Nucleoporin_Nup155_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042537">
        <property type="entry name" value="Nucleoporin_Nup155_C_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10350">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10350:SF6">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03177">
        <property type="entry name" value="Nucleoporin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08801">
        <property type="entry name" value="Nucleoporin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="9" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Nucleoporin Nup133/Nup155-like N-terminal" evidence="14">
        <location>
            <begin position="120"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin Nup133/Nup155-like C-terminal" evidence="13">
        <location>
            <begin position="730"/>
            <end position="1432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="511"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="516"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF03177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR22069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1438" mass="161294" checksum="CF7CF8DBAFEBA95B" modified="2009-07-28" version="1">MSIYSTPLKSGIDYTRNVVIPSNGELAAPNNTKRVEVNNEVVAMPGYNVLAPNASLGLASGEEHLRITGFASKPSLELASQYIDHLQNRDASTPVLDERSYYNNGVNYNFSKEVGGLGAFTPFERTQVMNVPDGILREASRTEMKSDMGLFPELNRCWITIDNKLILWNFNNPSDFQSIDDIKHTILAVALAKPKPNTFVESVNHLLLIATPFDVYVLAIQYDRSNEELSVFNTGMCVSVHGLDVSSFISCEQTGRIFFTGKSSGINIWELQYSSTEDWFNSRCNKVCLTQSVLSSLLPTNLISKIPGSNLVQSLFEESSKYSAEHLTQMCVDNSRGILYSLSNKSVIRAYKINGKSLGSPLTIETSYIKRIMGTTSARGAAILGNKYLKISKVVAVSGRENGNLFLVAITIGGVRLYFNGSLGRTHLEALRLESIKFPPSSVTPEAIEQELQQQQQKKSLPFYSSLTSSESVMLRFQKKSSVLLETSGASAIISPGIFLAAVTKAPNQETAKNAQDQVPTQQQPQQQRQSTYGIQQKLFVSVPDYGILKKHGKYVENTTLLDTTGVIKHIVPITPLFNATNKPEGYANEFATQYVSSEFKIAVLTNTSVEIYRYRTPDAVFEALAENPLPFLLNYGLTEACSTALFVTCKFNKSEALRSSALTFYAVGIPGVADIKPRYSRYTASAMSSLLSRPTIASTPQRSTLDTDKTGFATSREDENCSLDDVILSPRFYGTALFIARLFREIWEKPVFGTVPDAKFDNQSHFVKSSVGDKNLINSVSVSKGDLEYYLSSVMILNDFFDTYGNSISSISIPSFQTGKNIDRTEEVANQAENIAINSLIRLVQFMKEAFSFLNVLYEESDVEGYEGQFLAFKDIMKLLNLDVQSELSKLTFKDIFAPTDNTKNLLREILSSIINRSISRGGSIEHIATALQERCGSFCSSSDILGFRAMEHLRKAKEVGLRDYETSTYHLTCANKLFEKIVDTISIERLKDAVSTMLDLNYYPGTIEFLLNIANSMDKGKLAYQYVADGYLEQDPRKVYYEKRVLAYELVFETLVRVDELVSAAVSAGIGGMNNVEELSKLKEETYSTALRYNDKLFHYQLYDWLVSQNCQDKLLQLDTDFILAYLQEKSKGSLDISNLLWVYQSKRSNFYAAAQILYSLAVSDFEVPLGQRIEYLSRANGFCNGLCAPSQRQQMIHLSGMIQEIFDVASIQDDILTLVRNDIRVAADTKEELIKQLDGKILPVSDLFNDYADPLDYYEACLAIFKISDFRNNEEIISKWTELFDSLRTEINANGNVEESANFINLLTSVVVRVGRNVRTSEFVFPITELFPIICDLFYENLPNEHIKEGAIASIFISAGVSFDKFYYLLKNLIETADSVNNIYKKEMTWLIKEWYESDVKLRDLVSYDEIRNLKNYSIETDPIENKVKETGTSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEA1</accession>
    <name>C5DEA1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0C07502p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0C07502g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the arginase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552550.1">
        <property type="nucleotide sequence ID" value="XM_002552504.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEA1"/>
    <dbReference type="STRING" id="559295.C5DEA1"/>
    <dbReference type="GeneID" id="8291421"/>
    <dbReference type="KEGG" id="lth:KLTH0C07502g"/>
    <dbReference type="eggNOG" id="KOG2964">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039478_1_0_1"/>
    <dbReference type="InParanoid" id="C5DEA1"/>
    <dbReference type="OMA" id="GQRAIHN"/>
    <dbReference type="OrthoDB" id="288726at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008783">
        <property type="term" value="F:agmatinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033389">
        <property type="term" value="P:putrescine biosynthetic process from arginine, via agmatine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11592">
        <property type="entry name" value="Agmatinase_PAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.10">
        <property type="entry name" value="Ureohydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006035">
        <property type="entry name" value="Ureohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358">
        <property type="entry name" value="ARGINASE/AGMATINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358:SF28">
        <property type="entry name" value="HYPOTHETICAL ARGINASE FAMILY PROTEIN (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00491">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00116">
        <property type="entry name" value="ARGINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51409">
        <property type="entry name" value="ARGINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950345" evidence="2">
        <location>
            <begin position="19"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR22112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="425" mass="46021" checksum="B890B46035F300A0" modified="2009-07-28" version="1">MKVTWVGCLTSLAGSSLAYVISDQGHFSNHRTDLLDQVFGEVLASHDGSGAAEVRQRSFNLDEDEDPEFKAMCGFYQEHPVEFPDDDLSNDLELFAGIVSFAHVPIERCFGSRTDGKSPLYDIAIVGAPFDTSVSYRPGARFGPNAVRQGSRRLGGGILPVRGFPGSKLRKLDPYNAGYKIVDCGDVPMTPYDNRIALNQLYRGQRAIYNKTTLKSDLKAPKVITLGGDHTVTLMALKAASEHNGPLAVVHFDSHIDTWDPKVLGGGITKTQSINHGTFLHFAHERGYIARDKSMHVGIRAPFIAPTDDKHDRECGFQKIVARDFDILGFQEIVSQIKQRVGDLPVYITVDIDVLDLSVAPGTGTPEPGGLTSRELLTVLDGLEGLNVVGADVVEVSPAFDTNGDITSIVAAQVIDSILGLMTVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEU4</accession>
    <name>C5DEU4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0D09834p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0D09834g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SFR1/MEI5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553137.1">
        <property type="nucleotide sequence ID" value="XM_002553091.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEU4"/>
    <dbReference type="FunCoup" id="C5DEU4">
        <property type="interactions" value="17"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEU4"/>
    <dbReference type="GeneID" id="8295376"/>
    <dbReference type="KEGG" id="lth:KLTH0D09834g"/>
    <dbReference type="eggNOG" id="ENOG502S2TF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084833_0_0_1"/>
    <dbReference type="InParanoid" id="C5DEU4"/>
    <dbReference type="OMA" id="AQMASNY"/>
    <dbReference type="OrthoDB" id="27934at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018468">
        <property type="entry name" value="SFR1/Mei5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10376">
        <property type="entry name" value="Mei5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="43"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR22699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="215" mass="25205" checksum="2E0BFC52ACE30ACF" modified="2009-07-28" version="1">MAITNASLTIHRKPFKTPFVSSVKRGGSLGSKTLPSKEIIHPNRQASKEERIVNEQLNKVRRENTTLQNSLKILRKYERELEVLELIDKWRSICQAGMAFLLNSTLLKVNKMGGYEELIRKEVEAEKRKLEYQFAGRLQDEVDEVLESEEFKALPELEQQWLRRDLQQKVDSNEQLQQEEIKKLDLKLTESSNKQMTMRELANRLKVDYALVFEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEY8</accession>
    <name>C5DEY8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">Ribosome biogenesis protein NOP53</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0D10846g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">May play a role in ribosome biogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
            <location evidence="5">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
            <location evidence="5">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the NOP53 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553181.1">
        <property type="nucleotide sequence ID" value="XM_002553135.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEY8"/>
    <dbReference type="FunCoup" id="C5DEY8">
        <property type="interactions" value="719"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEY8"/>
    <dbReference type="GeneID" id="8295420"/>
    <dbReference type="KEGG" id="lth:KLTH0D10846g"/>
    <dbReference type="eggNOG" id="KOG2823">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035888_1_0_1"/>
    <dbReference type="InParanoid" id="C5DEY8"/>
    <dbReference type="OMA" id="TEKWTHK"/>
    <dbReference type="OrthoDB" id="5072at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008097">
        <property type="term" value="F:5S rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011687">
        <property type="entry name" value="Nop53/GLTSCR2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14211">
        <property type="entry name" value="GLIOMA SUPPRESSOR CANDIDATE REGION GENE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14211:SF7">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN NOP53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07767">
        <property type="entry name" value="Nop53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017302">
        <property type="entry name" value="Gltscr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="214"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="357"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="223"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="239"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="266"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="305"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="357"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR017302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR22743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="448" mass="51479" checksum="89DBB362FECF152F" modified="2009-07-28" version="1">MAAEKERPSQYRQSSRKGKKAWRKNIDISDIEKSIRQQQDEEITHGAKDLSSLRNEDLFQVDTVGDEVWKQKLIKRKQIKKNLKSSEILDSIKTNSKVAPLKHPKSQAAQDDEKKKIQGVSKKELRKLMALAGRIEGESKLKNAVSKKGLVRAGTNDLWGAEEEVKVPSGIKLATKDSTAIPEELKKLSTTGWSIATVAPDTLKRAPMKVKDVEEIPHAGKSYNPSSKEWTELVNSEYQSEKAREEARLQMQAYRERIKHLMETLDNNEEEDSEDDEDEADENQEPQENAEASSEIKLSLNKPSANKKKTKYQRNKQRRHQEKLSLQKELKQLKGQLRELERFEEIEKDVLEKQAAREAEKQTRVGKEKPNKKHKLGTKHSVIEGNLELKFSDELSDSLRKLRPEGNLLYDAAKKLQSSGKIETRIPVKKGRRYAPRITEKWTYKDLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEZ3</accession>
    <name>C5DEZ3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0D10956p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0D10956g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553186.1">
        <property type="nucleotide sequence ID" value="XM_002553140.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEZ3"/>
    <dbReference type="FunCoup" id="C5DEZ3">
        <property type="interactions" value="44"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEZ3"/>
    <dbReference type="GeneID" id="8295425"/>
    <dbReference type="KEGG" id="lth:KLTH0D10956g"/>
    <dbReference type="eggNOG" id="ENOG502S43T">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089696_4_1_1"/>
    <dbReference type="InParanoid" id="C5DEZ3"/>
    <dbReference type="OMA" id="GVWATKE"/>
    <dbReference type="OrthoDB" id="15433at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008897">
        <property type="term" value="F:holo-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.20">
        <property type="entry name" value="4'-phosphopantetheinyl transferase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008278">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037143">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01648">
        <property type="entry name" value="ACPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56214">
        <property type="entry name" value="4'-phosphopantetheinyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950446" evidence="2">
        <location>
            <begin position="24"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="4'-phosphopantetheinyl transferase" evidence="3">
        <location>
            <begin position="32"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR22748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="196" mass="22306" checksum="4E8D225BD6A7A007" modified="2009-07-28" version="1">MPMKRISSRLLNKRMLLLPATLAIPPHAAMLGIGTDIVYLPRFAKIVEKHAPVGQSTTFRKIATKFMHPEEVRHLYQMLQLGYNSPSVVRYLAGVWATKEAIFKSLAAQRLEIPLLPAQKIYTQVFYKTNEQDGAPRVEFDSSIERLHPEFARAYIANTRFVLSIAHDEDYLVSFVCHIRGQAAENVVQKTPRPFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DF03</accession>
    <name>C5DF03_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 16">Diacylglycerol O-acyltransferase</fullName>
            <ecNumber evidence="5 16">2.3.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">KLTH0D11198g</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 16">
            <text>an acyl-CoA + a 1,2-diacyl-sn-glycerol = a triacyl-sn-glycerol + CoA</text>
            <dbReference type="Rhea" id="RHEA:10868"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:64615"/>
            <dbReference type="EC" id="2.3.1.20"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 16">Glycerolipid metabolism; triacylglycerol biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Endoplasmic reticulum membrane</location>
            <topology evidence="1 16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 16">Belongs to the diacylglycerol acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.20" evidence="5 16"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553196.1">
        <property type="nucleotide sequence ID" value="XM_002553150.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DF03"/>
    <dbReference type="FunCoup" id="C5DF03">
        <property type="interactions" value="249"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DF03"/>
    <dbReference type="GeneID" id="8295436"/>
    <dbReference type="KEGG" id="lth:KLTH0D11198g"/>
    <dbReference type="eggNOG" id="KOG0831">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023995_4_1_1"/>
    <dbReference type="InParanoid" id="C5DF03"/>
    <dbReference type="OMA" id="RSQWMRR"/>
    <dbReference type="OrthoDB" id="264532at2759"/>
    <dbReference type="UniPathway" id="UPA00282"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004144">
        <property type="term" value="F:diacylglycerol O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006071">
        <property type="term" value="P:glycerol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07987">
        <property type="entry name" value="LPLAT_MGAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007130">
        <property type="entry name" value="DAGAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12317:SF0">
        <property type="entry name" value="ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12317">
        <property type="entry name" value="DIACYLGLYCEROL O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03982">
        <property type="entry name" value="DAGAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 16" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="10 16" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0319">Glycerol metabolism</keyword>
    <keyword evidence="6 16" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="12 16" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="137"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="30"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="51"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU367023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAR22758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="499" mass="57470" checksum="73B2A2B0D0F3C803" modified="2009-07-28" version="1">MCKTLNRTKQLAERLLRGHLSWAEVDNTADGLKRSDGRTATMDNSKGLKQRGREGGRENGGEHGAEHRDENAASERSSKASGFLHHELDHVRLYDRNHQEGKKLSEFVEEINHFQEPRPKLAFCSVKTPWRRRLQTLAVAWHVASFVYILAFAILALANPIMWIIMFPYMAYYAFDRSPANGNVTKRYSKWMRGLTFWRYYCDYFPIQLHKTADLKPTFTHKKHSSGCPSEDKYLVSFQLRLWPLRYSLKVNILKLGGAPPREVTGPRYIFGYHPHGVAALGAFGAFATEGANWSKLFPGIPMCLMTLVNQFQIPFYRDYLLSLGVTTVSKKNALKVLERNHSICIVVGGAQEALMSKLGSADLVLKRRKGFIKLALETGNVCLVPCFAFGETDCYNILETDEKSYLHKFQLWFKKTYGFTIPLFFARGLFNYDFGLIPFRKPINVVTGNPIYIKRKHDNPTVEEIDHYQKLYIDELQRTFDDYKRQFGYKDLELNLAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DF81</accession>
    <name>C5DF81_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0D12936p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0D12936g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553274.1">
        <property type="nucleotide sequence ID" value="XM_002553228.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DF81"/>
    <dbReference type="FunCoup" id="C5DF81">
        <property type="interactions" value="231"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DF81"/>
    <dbReference type="GeneID" id="8295514"/>
    <dbReference type="KEGG" id="lth:KLTH0D12936g"/>
    <dbReference type="eggNOG" id="KOG0191">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059951_0_0_1"/>
    <dbReference type="InParanoid" id="C5DF81"/>
    <dbReference type="OMA" id="QVASVNC"/>
    <dbReference type="OrthoDB" id="10264505at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03002">
        <property type="entry name" value="PDI_a_MPD1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45815">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE A6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45815:SF3">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE A6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00421">
        <property type="entry name" value="THIOREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950448" evidence="2">
        <location>
            <begin position="19"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="3">
        <location>
            <begin position="7"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="292"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="293"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR22836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="311" mass="36396" checksum="C408CA12D626A24A" modified="2009-07-28" version="1">MLLQRLWLVFLGLCVVWAQNFYDNSPHIMELTPKSFDKVVHKTNYTTLVEFYAPWCGYCKMLKPILQRAAKNLDGIVQVASVNCDLAKNKQLCAKYRVEGFPILMVFRPPKVDLSKKGKDRLKLGNHASEVYKGERKLRPIVDFSISRVKNYITRITRLEKLVQHLNRNNEVRYKAVLFSKKDKISPLYKSLALDWLGSVDFSLFLNSKLSTTPEALRDLHPEFQNFLSELRENANDLEKSMLVLFDTQDHRYYIYEEESFEKVNIWKLLSKFASPNEGPFTKRQDFLDALKSNKKTRKPPAKQKLEHDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5DF99</accession>
    <name>C5DF99_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transcription elongation factor SPT4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 13">Chromatin Elongation factor SPT4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Transcription elongation factor spt4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0D13398g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SPT4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553292.1">
        <property type="nucleotide sequence ID" value="XM_002553246.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DF99"/>
    <dbReference type="FunCoup" id="C5DF99">
        <property type="interactions" value="478"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DF99"/>
    <dbReference type="GeneID" id="8295534"/>
    <dbReference type="KEGG" id="lth:KLTH0D13398g"/>
    <dbReference type="eggNOG" id="KOG3490">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_138052_2_1_1"/>
    <dbReference type="InParanoid" id="C5DF99"/>
    <dbReference type="OMA" id="FDGMIAV"/>
    <dbReference type="OrthoDB" id="248751at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032044">
        <property type="term" value="C:DSIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140673">
        <property type="term" value="P:transcription elongation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07973">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.210:FF:000003">
        <property type="entry name" value="Transcription elongation factor SPT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029040">
        <property type="entry name" value="RPABC4/Spt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009287">
        <property type="entry name" value="Spt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022800">
        <property type="entry name" value="Spt4/RpoE2_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038510">
        <property type="entry name" value="Spt4_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12882">
        <property type="entry name" value="SUPPRESSOR OF TY 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12882:SF1">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR SPT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06093">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF025023">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01389">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63393">
        <property type="entry name" value="RNA polymerase subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0137">Centromere</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Spt4/RpoE2 zinc finger" evidence="14">
        <location>
            <begin position="3"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00070621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00080334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR22854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="101" mass="11072" checksum="3F6B5B34A2861FB8" modified="2009-07-28" version="1">MSERACMLCGIVQTTAEFTKDGCPNCQGVFDEAGVSSMECTSPSFEGLVGMCKPSKSWVAKWISVDQYIPGMYAIKVDGRLPVEVFDLLPHYKPRDGSQVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFC5</accession>
    <name>C5DFC5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0D14014p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0D14014g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553318.1">
        <property type="nucleotide sequence ID" value="XM_002553272.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFC5"/>
    <dbReference type="FunCoup" id="C5DFC5">
        <property type="interactions" value="227"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFC5"/>
    <dbReference type="GeneID" id="8295562"/>
    <dbReference type="KEGG" id="lth:KLTH0D14014g"/>
    <dbReference type="eggNOG" id="KOG1470">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016665_3_0_1"/>
    <dbReference type="InParanoid" id="C5DFC5"/>
    <dbReference type="OMA" id="ISTMRWR"/>
    <dbReference type="OrthoDB" id="43460at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052432">
        <property type="entry name" value="PITP/CRAL-TRIO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46590:SF1">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL TRANSFER PROTEIN CSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46590">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL TRANSFER PROTEIN CSR1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03765">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01100">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="2">
        <location>
            <begin position="192"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="79"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR22880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="440" mass="50158" checksum="639AFAD1EE78233A" modified="2009-07-28" version="1">MSVEVAKGRVQTLTQDEELHLKQVWAYLFQHWGVQVNSASVFASETAGSGQSGSSGGNKNAEEKPAKKSGKLFGRFRKSSSNSSAAKSSRESNGAVPASSQRSTEPSEGARSHGTVHEALKNLKPDEISENFWDMLRSDYPDNLILRFLRARKWDTDKALGMIAHTLEWRLKEGHPDSIIRGGEQAAYQNNETGYIKNLELSKATQRGFDLHGRPIVLVRPRLHHSSDQTEEEMKKYCLLIIEQARMFLKEPVDSATILFDLTGFSMSNMDYTPVQYLISCFEAHYPECLGHLFIHKAPWIFPPIWNIIKNWLDPVVASKIVFTKNASDLAKYVPMKYIPSYLGGEDEYDFDSFKRPDESADVKLKDTKTRDSILEKRKSIIQQFIDSTKNWIESQNDEESKKWLETKISISKELSENYRELDPYIRSRSVYDINGTLKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFK3</accession>
    <name>C5DFK3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0D15818p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0D15818g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553396.1">
        <property type="nucleotide sequence ID" value="XM_002553350.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFK3"/>
    <dbReference type="FunCoup" id="C5DFK3">
        <property type="interactions" value="614"/>
    </dbReference>
    <dbReference type="GeneID" id="8295643"/>
    <dbReference type="KEGG" id="lth:KLTH0D15818g"/>
    <dbReference type="eggNOG" id="KOG2164">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011811_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFK3"/>
    <dbReference type="OMA" id="PRWKKCP"/>
    <dbReference type="OrthoDB" id="302966at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039739">
        <property type="entry name" value="MAG2/RNF10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12983:SF9">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12983">
        <property type="entry name" value="RING FINGER 10 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="9">
        <location>
            <begin position="160"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="8">
        <location>
            <begin position="166"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="507"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="18"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="46"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="539"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="602"/>
            <end position="612"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR22958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="612" mass="69423" checksum="20AE8447916A8408" modified="2009-07-28" version="1">MSQENSASATSGSPGNKKFSHKEKPKKPASHRQHRVNSKPPKTGKHRDGPGKQRFEDDLEFNIQDELVSGDFKARGRKTQISINHLLDFQLPERPSDGEAAAGATRRRTKKQFQEHIHLHGDSFINANFKFLVDDRYTYKEQSLDPNVPIPHEKIVRVTATKGQSCPICLTEEIVAPRMVSCGHLFCHACLLSFFAAGMEQPNNNGTGINYKKKRYKECPLCSTIIRKEKAIPATLTEPDSTLKLPQVGQTDTFQLMCKPHGSMLPLPVLLNVDPMSLHHFPSVEFRELAPYSRIMKCNPQYAIDLYKKDISHIQGQYELDRALYNEDGKFSQLAIEENKLKISVSEQSFRSEAEVLSSNLSQLSLDVGLEKYNDTNAFFFFQTAFDANTRYYLSPLDVKVLLTAFGHYSAFPPSLTAQVEDVHYGTVVTESLMQRYKYFGHLPIGSEMAFVDLDWRTADVIPSEVYQSFAAELSLRRRKSTWRKQREDKEKKRYERKLEREQQEFYMRENGSTDHATSVIKPVRSVPLQKLPEKKSDTGSAASAQKSASRLEKTVWGTSIPASDKAPEGNQEDEELEKLLGNLNQNPSPRQGRKKKRMVTLLSSNPSRGSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFP9</accession>
    <name>C5DFP9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="8">PPIase</shortName>
            <ecNumber evidence="8">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0D16918g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the cyclophilin-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="8"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR23004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553442.1">
        <property type="nucleotide sequence ID" value="XM_002553396.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFP9"/>
    <dbReference type="FunCoup" id="C5DFP9">
        <property type="interactions" value="415"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFP9"/>
    <dbReference type="GeneID" id="8295691"/>
    <dbReference type="KEGG" id="lth:KLTH0D16918g"/>
    <dbReference type="eggNOG" id="KOG0865">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012062_4_3_1"/>
    <dbReference type="InParanoid" id="C5DFP9"/>
    <dbReference type="OMA" id="FNQDMQM"/>
    <dbReference type="OrthoDB" id="193499at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016018">
        <property type="term" value="F:cyclosporin A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000032">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024936">
        <property type="entry name" value="Cyclophilin-type_PPIase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF327">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE C, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001467">
        <property type="entry name" value="Peptidylpro_ismrse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0697">Rotamase</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="9">
        <location>
            <begin position="23"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR23004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="180" mass="19699" checksum="E5FA3211E78C25F4" modified="2009-07-28" version="1">MLSTVRQSRMFSSTAAKLGSKVFFDTSINGTKQPRIEFELYDDIVPKTAANFKALCTGEKGFGYKGVPFHRIIPNFMLQGGDTDLTNGYGGRSIYGHKFQDENFVKKHEKAGLLSMANAGPNTNGSQFFITTVPCPWLDGKHVVFGEVSKGMDVVKTIESYGTMSGKPRAKVVVEEAGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFS1</accession>
    <name>C5DFS1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 16">Homoserine dehydrogenase</fullName>
            <ecNumber evidence="5 16">1.1.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ordered locus">KLTH0D17446g</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the conversion of L-aspartate-beta-semialdehyde (L-Asa) to L-homoserine (L-Hse), the third step in the biosynthesis of amino acids that derive from aspartate (the aspartate family of amino acids), including methioinine and threonine, the latter of which is a precursor to isoleucine; production of homoserine leads to a branch-point in the pathway as it can either be O-phosphorylated for processing to threonine, or O-acylated for processing to methionine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-homoserine + NADP(+) = L-aspartate 4-semialdehyde + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15761"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="1.1.1.3"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="12">
            <dbReference type="Rhea" id="RHEA:15763"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:25213"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 16">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 3/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 16">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">Belongs to the homoserine dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.3" evidence="5 16"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR23026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553464.1">
        <property type="nucleotide sequence ID" value="XM_002553418.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFS1"/>
    <dbReference type="FunCoup" id="C5DFS1">
        <property type="interactions" value="393"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFS1"/>
    <dbReference type="GeneID" id="8295714"/>
    <dbReference type="KEGG" id="lth:KLTH0D17446g"/>
    <dbReference type="eggNOG" id="KOG0455">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009116_0_1_1"/>
    <dbReference type="InParanoid" id="C5DFS1"/>
    <dbReference type="OMA" id="IYTRCYS"/>
    <dbReference type="OrthoDB" id="67851at2759"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00063"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00465"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004412">
        <property type="term" value="F:homoserine dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009090">
        <property type="term" value="P:homoserine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000006">
        <property type="entry name" value="Bifunctional aspartokinase/homoserine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005106">
        <property type="entry name" value="Asp/hSer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011147">
        <property type="entry name" value="Bifunc_Aspkin/hSer_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001342">
        <property type="entry name" value="HDH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019811">
        <property type="entry name" value="HDH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022697">
        <property type="entry name" value="HDH_short"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43070:SF5">
        <property type="entry name" value="HOMOSERINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00742">
        <property type="entry name" value="Homoserine_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03447">
        <property type="entry name" value="NAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036497">
        <property type="entry name" value="HDH_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01042">
        <property type="entry name" value="HOMOSER_DHGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 16" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11 16" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="9 15 16" id="KW-0521">NADP</keyword>
    <keyword evidence="10 16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 16" id="KW-0791">Threonine biosynthesis</keyword>
    <feature type="domain" description="Aspartate/homoserine dehydrogenase NAD-binding" evidence="19">
        <location>
            <begin position="12"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Homoserine dehydrogenase catalytic" evidence="18">
        <location>
            <begin position="150"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="14">
        <location>
            <position position="222"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="12"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>NADPH</name>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>NADPH</name>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>L-homoserine</name>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00059589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR036497-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR036497-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU004171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF03447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CAR23026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="358" mass="38647" checksum="1896FDDBD26CA9F6" modified="2009-07-28" version="1">MASKSVNLAVIGAGVVGSSFLKQVSGVKSNISYNLILIATSDNFLISRDYKPLSLSTWESDLTSSKDSPLPLEGILEYLKKSSSPVIFVDNTSNQTIAEFYPQLVQNGISIATPNKKGFSSDLSLWNKIFQEGPNAGLVYHEATVGAGLPIIGPLRDMVQTGDKIVKIEGIFSGSLSFILNEFSTISGNDSKFSDIVKVAKNLGYTEPDPRDDLNGLDVARKVTILARIAGFKVESPSSFPVKSLIPKPLESVASGKEYLEKLPAYDHEMDELKKEAAAENKVLRFVGKVDFPNNTVSVAMEKYDFSHPFASLKGSDNVISIQTERYTQPVIVQGAGAGSEVTAAGVLADVFRIAERI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5DG99</accession>
    <name>C5DG99_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0D03542p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0D03542g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552879.1">
        <property type="nucleotide sequence ID" value="XM_002552833.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DG99"/>
    <dbReference type="FunCoup" id="C5DG99">
        <property type="interactions" value="52"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DG99"/>
    <dbReference type="GeneID" id="8295101"/>
    <dbReference type="KEGG" id="lth:KLTH0D03542g"/>
    <dbReference type="eggNOG" id="KOG1929">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020751_0_0_1"/>
    <dbReference type="InParanoid" id="C5DG99"/>
    <dbReference type="OMA" id="ERCLHYK"/>
    <dbReference type="OrthoDB" id="251770at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033314">
        <property type="term" value="P:mitotic DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007095">
        <property type="term" value="P:mitotic G2 DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00027">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13561">
        <property type="entry name" value="DNA REPLICATION REGULATOR DPB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13561:SF20">
        <property type="entry name" value="DNA TOPOISOMERASE 2-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12738">
        <property type="entry name" value="PTCB-BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BRCT" evidence="3">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="3">
        <location>
            <begin position="167"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="659"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="659"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="675"/>
            <end position="688"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR22441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="707" mass="79656" checksum="320FE8B473283A88" modified="2009-07-28" version="1">MQKPLQGITFCPTALPEDASRSVSRKVAKLGGGFSKDLTKLVNVLVVGSVATNKYRFAVQNRADMAFVGVDAIDTIYDLWLAGDDISMESHSNFSGIKNPRERMLSVLRTRHQLGALKDFVVFIGRVGDDQEGKLSPDSLERLCVEQGVHSCNTRHFVKESHWNRPTVFVTDCSRGARVDAARSQGLPIVHPKWVLDCHNRSALLDFAYYLLENNEHVSFEVVGAGSCMCWDQISFQPSSAAANAHEDDSRLSQRVVLDKFASDGHRLWNSVMHKTKKISPAETFPLPNAEKTKELPGLFHDKHCYLLGFPQKHKAVLHNIIVRNSGSCYDYEDGVQVREPSYLVIPSNVPRYTPASVDELFTHVVTEFFFERCLHYKKLLKPDPWCSPFFSNFEVIPSDHLRQNLTVSEPLKVAITGFQGVELLHITKILEYLEPSGLKLSQKLNKETNVLIINLGALNSIPESHPLWKNQYASMFGEGKKLEQNQIHRNSMKRKIEFIKQRHSIPVVTAAFILELFSRVSKAKAQLKVSSRIHLNDVNWCISCPKGNKDQFYCDLLAKETDSTDSGLEIASSANDCVNRSIKQNRQEALDIFRNGPTVNSKRSLYEEGIEARHSAEKVPSPLPKIPRLVPTEALKPVQRSSSWGNLLSDQARKVDGLHDSDVEPSTGERELQHTQVTYGSPKQINDVQAPPTRRLTRQRMKEIDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGH7</accession>
    <name>C5DGH7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="21">KLTH0D05412p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="21" type="ordered locus">KLTH0D05412g</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="17">Component of the 850 kDa m-AAA protease complex, a heterohexamer composed of YTA12/RCA1 and YTA10/AFG3. Associates with the prohibitin complex, composed of PHB1 and PHB2, inhibiting the activity of the m-AAA protease complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the peptidase M41 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552957.1">
        <property type="nucleotide sequence ID" value="XM_002552911.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGH7"/>
    <dbReference type="FunCoup" id="C5DGH7">
        <property type="interactions" value="710"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGH7"/>
    <dbReference type="MEROPS" id="M41.003"/>
    <dbReference type="GeneID" id="8295185"/>
    <dbReference type="KEGG" id="lth:KLTH0D05412g"/>
    <dbReference type="eggNOG" id="KOG0731">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_23_0_1"/>
    <dbReference type="InParanoid" id="C5DGH7"/>
    <dbReference type="OMA" id="ARQKGNF"/>
    <dbReference type="OrthoDB" id="1413014at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005745">
        <property type="term" value="C:m-AAA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097002">
        <property type="term" value="C:mitochondrial inner boundary membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140567">
        <property type="term" value="F:membrane protein dislocase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034982">
        <property type="term" value="P:mitochondrial protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065003">
        <property type="term" value="P:protein-containing complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19501">
        <property type="entry name" value="RecA-like_FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000019">
        <property type="entry name" value="AFG3-like AAA ATPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.760:FF:000003">
        <property type="entry name" value="AFG3-like AAA ATPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000001">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1690.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.760">
        <property type="entry name" value="Peptidase M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01458">
        <property type="entry name" value="FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050928">
        <property type="entry name" value="ATP-dep_Zn_Metalloprotease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005936">
        <property type="entry name" value="FtsH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011546">
        <property type="entry name" value="Pept_M41_FtsH_extracell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000642">
        <property type="entry name" value="Peptidase_M41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037219">
        <property type="entry name" value="Peptidase_M41-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01241">
        <property type="entry name" value="FtsH_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43655">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43655:SF14">
        <property type="entry name" value="MITOCHONDRIAL RESPIRATORY CHAIN COMPLEXES ASSEMBLY PROTEIN YTA12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06480">
        <property type="entry name" value="FtsH_ext"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01434">
        <property type="entry name" value="Peptidase_M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140990">
        <property type="entry name" value="FtsH protease domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="167"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="20">
        <location>
            <begin position="375"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="38"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="102"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="47"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00065348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="CAR22519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="813" mass="91437" checksum="D5D58C50EFCAFCE3" modified="2009-07-28" version="1">MSTLLRPYSRLLWARLRCTRAFTSPRFTPMYKIARLHTNPSLFQKPPSDKGSNKDANDEDLENVRREVERYIEEAKNDKSVDWEQRKKRIDEGIARLEETILRQQNKHHDEPKEQSGREAEPSESKEEGPSEFEKKQAFEQNKKLNDRKKAEEEKLSKIMNDPNARVLAVNIGFLEIGVLLFLLSFLLNRLYNTEEQREITWQEFRTKLLAKGYVAKLVVVNKSFVKVVLNENGKNHPEHNGHDFYFFTIGSVESFEHKLQRAQEEIGIAEDFKTPVVYVQEGNWAKAMYQILPTALMIGGLIWITKRSASGAGGGGPGGIFSVGKSKAKRFNKDTDVKVTFKDVAGCDEAKEEIMEFVSFLKEPSRYEKMGAQIPRGAILSGPPGTGKTLIAKATAGEAGVPFFSVSGSEFVEMFVGVGASRVRDLFKTARENAPSIVFVDEIDAIGKARQKGNFSGANDERENTLNQLLVEMDGFTPSDHVVVLAGTNRPDVLDQALMRPGRFDRHINIDRPELEGRKQIFQVHLAKVKLAGSMEDLKNRLAALTPGFSGADISNVCNEAALTAARNECTSVKLEHFEQAIERVIGGVERKSKLLSPEEKKIVAYHEAGHAVCGWYLEFADPLLKVSIIPRGQGALGYAQYLPGDVYLLSEQQLKDRMTMALAGRVSEELHFPSVTSGASDDFQKVTRMATAMVTELGMSDKIGWLNYQKKSESDLTKPFSEETAAIVDAEVFRLVQECHDRCTALLKEKAEDVEKITQLLLEKEVLTREDMIRLLGKRPFPERNDAFDKYLNESETKRLRKNEADQGMSS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-05-03" modified="2025-06-18" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGI8</accession>
    <name>MTC2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName>Maintenance of telomere capping protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">MTC2</name>
        <name type="ordered locus">KLTH0D05654g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in telomere capping.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MTC2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552968.1">
        <property type="nucleotide sequence ID" value="XM_002552922.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGI8"/>
    <dbReference type="FunCoup" id="C5DGI8">
        <property type="interactions" value="128"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGI8"/>
    <dbReference type="GeneID" id="8295196"/>
    <dbReference type="KEGG" id="lth:KLTH0D05654g"/>
    <dbReference type="eggNOG" id="ENOG502QQMN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060779_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGI8"/>
    <dbReference type="OMA" id="WFACAEP"/>
    <dbReference type="OrthoDB" id="5582146at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.80">
        <property type="entry name" value="Magnesium chelatase subunit I, C-Terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000407763" description="Maintenance of telomere capping protein 2">
        <location>
            <begin position="1"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="285" mass="32501" checksum="69576F0DC87E4FFD" modified="2009-07-28" version="1">MSGQEPSLPPMGPTLPAALAFRKNLVCYCEDPTVVVEFLQALHLPYALWENYDDIDARPKPEAEQVLVLPNVDRLLTLQQDKLAKFLQVMRSRDTPFFAAVATVSPGFVPYAHFTPYLKRQFWFACAEPMTGGATDFNEETLPQLRNSLRKVHVHHSIRRYVLDIIMHLRVHRFSSQASGGGCSTHSLRDILELCQTLALAEERAFVVPDIVKTASYWYFPFHLELIQNPSHEISLQYGSDPDLVAQLVNAMQQFSLQRASEIHYPLYFQYMVLRDVLNLVVPAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGJ7</accession>
    <name>C5DGJ7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0D05852p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0D05852g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552977.1">
        <property type="nucleotide sequence ID" value="XM_002552931.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGJ7"/>
    <dbReference type="FunCoup" id="C5DGJ7">
        <property type="interactions" value="924"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGJ7"/>
    <dbReference type="GeneID" id="8295205"/>
    <dbReference type="KEGG" id="lth:KLTH0D05852g"/>
    <dbReference type="eggNOG" id="KOG1969">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004894_3_1_1"/>
    <dbReference type="InParanoid" id="C5DGJ7"/>
    <dbReference type="OMA" id="RWLKGWE"/>
    <dbReference type="OrthoDB" id="2195431at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18140">
        <property type="entry name" value="HLD_clamp_RFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047854">
        <property type="entry name" value="RFC_lid"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23389">
        <property type="entry name" value="CHROMOSOME TRANSMISSION FIDELITY FACTOR 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23389:SF3">
        <property type="entry name" value="CHROMOSOME TRANSMISSION FIDELITY PROTEIN 18 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0235">DNA replication</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="4">
        <location>
            <begin position="155"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR22539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="702" mass="79892" checksum="1B6C2B482CBFD41C" modified="2009-07-28" version="1">MEGRLEPLIGDSMLFGPMEDQGLSFVNKSGETVHLRRKPARALMDLNWKHDESYGIDINQLLQRCEDSSNAAIQSSGASRSKHSPSQLWTEKWKPHTFFDLVGNEKTNRRVLKWLRQWSPLVFGQELPKKAPSVRDKVGADGANYDFDDPLQRPQKRILLLNGPPGIGKTSVAHVVAKQAGFSVIEINASDERAGPHVKDRIHNALFNHTFDDRPVCLIADEIDGSIETGFVKVLIDIVNSDYRATQRLASGASSAVQRGKRKQRSPGKVLTRPIITICNNVYASALEKLRPHCEIVTFRRPAESALLERLSHVCRKEKLQLDKKRLKELSELSQGDLRSCLNNMQFMASLHAEAPESTDTAKISDQIKDMTVSWYKICNQVFRRNPHEDAKVQFGRLLRDIEINSNSQRIVQGCFSMFPEVKFSDHSLQKPSAAADWLYFNDLMFKSLFEHNGDLLRYNSVVPMAFFHLFSDIANKDDVRGKVNEFDHREDLRNNESIVLSTFRRAPPTLKIFMNKSSLALEVLPLLDYIIVPDFSKIRNNQTKTTVLSNIVAALSSFDLTFSERVDTDLPRVTCIEPPFDKVTLMDEKRVKEVFTKRPAILNVVLAKAQESKVRKRTIEQARADKLDLESVRKKHKTSSSKPVDYFKSQYANVQTKGSEKFKDSGSVEGDSREPVKIWVKYKEGFSDAVRKDVTWNSLWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGJ8</accession>
    <name>C5DGJ8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0D05918p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0D05918g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552978.1">
        <property type="nucleotide sequence ID" value="XM_002552932.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGJ8"/>
    <dbReference type="FunCoup" id="C5DGJ8">
        <property type="interactions" value="334"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGJ8"/>
    <dbReference type="GeneID" id="8295208"/>
    <dbReference type="KEGG" id="lth:KLTH0D05918g"/>
    <dbReference type="eggNOG" id="ENOG502S2TX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064108_0_0_1"/>
    <dbReference type="InParanoid" id="C5DGJ8"/>
    <dbReference type="OrthoDB" id="4940293at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14688">
        <property type="entry name" value="bZIP_YAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050936">
        <property type="entry name" value="AP-1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40621:SF8">
        <property type="entry name" value="AP-1-LIKE TRANSCRIPTION FACTOR YAP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40621">
        <property type="entry name" value="TRANSCRIPTION FACTOR KAPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="6">
        <location>
            <begin position="135"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="129"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR22540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="310" mass="34787" checksum="80CE92B3C35A9550" modified="2009-07-28" version="1">MMDYSNNFSYSNQYQSSGQAQAVEQGFGADLHSCSNDDCSAASNLFDGLTEENKTFHEILAANSNTITPPGSSSPGYQQDLLSSMKTVKRGTEINFDCLEGEAPQNLIPEALASAQGFKADTVNSFQQDSNPIEDASARKKKAQNRAAQRAFRERKEAKMKQLQEQLVTSERDKQSLLRQMEELRERNLKMLTENHLLLQKNNSNLPTQSNSGLASASRKFHFPSQSEFIESAIEGHEVPVKLPLSSTYYEHDGEELLTFPATWEYLHKLSEKEDFDVYSVMLNLKGHEVCHGYGPAYRKTVIDKLVKQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGX4</accession>
    <name>C5DGX4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0D09042p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0D09042g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid/polyamine transporter 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553104.1">
        <property type="nucleotide sequence ID" value="XM_002553058.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGX4"/>
    <dbReference type="FunCoup" id="C5DGX4">
        <property type="interactions" value="598"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGX4"/>
    <dbReference type="GeneID" id="8295341"/>
    <dbReference type="KEGG" id="lth:KLTH0D09042g"/>
    <dbReference type="eggNOG" id="KOG1304">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009646_3_2_1"/>
    <dbReference type="InParanoid" id="C5DGX4"/>
    <dbReference type="OMA" id="YWCYYIL"/>
    <dbReference type="OrthoDB" id="1684102at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005302">
        <property type="term" value="F:L-tyrosine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF666">
        <property type="entry name" value="VACUOLAR AMINO ACID TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="306"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="332"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="378"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="410"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="446"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="488"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="520"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="561"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="628"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="652"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="695"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="9">
        <location>
            <begin position="300"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="112"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="211"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="112"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="213"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR22666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="718" mass="79839" checksum="01C6BCBF7AAEEE06" modified="2009-07-28" version="1">MKPKTKKSDGIDSLSSNISIPQQQDRRRHSILLLSESVASRRSSYTRALRDSKWRKSGTFSKSPQLGRSVDSRIFIDRASPSFKTPKQQHQEEILNSVRTNYLNDYMSRKASQTSLSLKGQNESSDTEQGPELLDRDPNLESQGGHITRELYRMTSPAAFGKAVKKTRSADDLTLEEQHSASNASALNVPGGFRREFILQKHNKGKTIDTALSSESETLHSSSVSGESVPDSSEKVPFLTRNFLEFLYVYGHFAGESFEDEFYTDQESEEQAVDETSPLLARAEAGTGKKILVPKSAKGTTSTTKAFLLMIKSFVGTGVLFLPGAFSNGGLFFSLAMLVFFGIYSYWCYYILTKSKIATKVSSFGDIGLTLYGPWMKFIILFSLVMTQLGFSGAYVVFTAKNLLAFVENVFYWPGVTIVHLLALQLVVFIPLSFIRNIAKLSFSSLVANFLVMGGIVIVIGFTAKHLFFDLNCKPAEGIVTGFNSQSWTLFVGTAIFAFEGIGLIIPIQSSMKHPEKFPLVMALVIITATVLFVSVATLGYLSYGAETQTVILLNLPQDSILVNLIQFFYSSAILLSTPLQLFPAIAIIENKVFPKFTKIYVKRSDHTKIQYKPNSGKLDWRIKWLKNFVRSLIVSSVVIAAYFGANHLDAFVAIVGSLACIPLVYIYPPMLHLRSCSKPRFAGEKSVWRKWPILLDYVLVVFGAIGMVYTSYQSIFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>C5DH09</accession>
    <name>C5DH09_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0E00418p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0E00418g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the D-glutamate cyclase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553507.1">
        <property type="nucleotide sequence ID" value="XM_002553461.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DH09"/>
    <dbReference type="GeneID" id="8291643"/>
    <dbReference type="KEGG" id="lth:KLTH0E00418g"/>
    <dbReference type="eggNOG" id="ENOG502QV7A">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059759_1_0_1"/>
    <dbReference type="InParanoid" id="C5DH09"/>
    <dbReference type="OMA" id="SHIAADC"/>
    <dbReference type="OrthoDB" id="10262538at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047820">
        <property type="term" value="F:D-glutamate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006536">
        <property type="term" value="P:glutamate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1640.10">
        <property type="entry name" value="PSTPO5379-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009906">
        <property type="entry name" value="D-Glu_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038021">
        <property type="entry name" value="Putative_hydro-lyase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32022">
        <property type="entry name" value="D-GLUTAMATE CYCLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32022:SF10">
        <property type="entry name" value="D-GLUTAMATE CYCLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07286">
        <property type="entry name" value="D-Glu_cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160920">
        <property type="entry name" value="PSTPO5379-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR23070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="204" mass="23246" checksum="AE1CBC61B361CDBA" modified="2009-07-28" version="1">MSNPESFRELCRKEIHTGPTSGVLPGYIQANLLILPKCVAESFEDLCTRNPVACPILSKTEGRPQFLRNHASLIKQGTFDIRTDFPRYNVYSRGKLVQQKNNCVNEWSSDHVGFLIGCSFTFEHALEKAGLVPKNVLYNKNVSMFRTTKFLDSAGIFHQCPYVVSMRPYKLTEVEIVREITRKFKKTHGEPLDWGYDGAKRLGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DH97</accession>
    <name>C5DH97_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0E02486g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the ZNF598/HEL2 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553595.1">
        <property type="nucleotide sequence ID" value="XM_002553549.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DH97"/>
    <dbReference type="FunCoup" id="C5DH97">
        <property type="interactions" value="105"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DH97"/>
    <dbReference type="GeneID" id="8291737"/>
    <dbReference type="KEGG" id="lth:KLTH0E02486g"/>
    <dbReference type="eggNOG" id="KOG2231">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008515_2_0_1"/>
    <dbReference type="InParanoid" id="C5DH97"/>
    <dbReference type="OMA" id="HTTCHKC"/>
    <dbReference type="OrthoDB" id="3838338at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16615">
        <property type="entry name" value="RING-HC_ZNF598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057634">
        <property type="entry name" value="PAH_ZNF598/HEL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041888">
        <property type="entry name" value="RING-HC_ZNF598/HEL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056437">
        <property type="entry name" value="Znf-C2H2_ZNF598/HEL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044288">
        <property type="entry name" value="ZNF598/HEL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22938:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE ZNF598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22938">
        <property type="entry name" value="ZINC FINGER PROTEIN 598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23202">
        <property type="entry name" value="PAH_ZNF598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25447">
        <property type="entry name" value="RING_ZNF598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23230">
        <property type="entry name" value="zf-C2H2_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="14">
        <location>
            <begin position="56"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="323"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="483"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="538"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="323"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="343"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="515"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="578"/>
            <end position="591"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00035113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR23158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="619" mass="70011" checksum="DBB757F43AAF3152" modified="2009-07-28" version="1">MSTQPQQKRNFRRTQGAQSKPKKDLGKNGEQGWKRTFSQGREGGDTASELDEEDICIICAEKIKYASISPCNHVTCNRCTFRQRALYNKRSCLVCRTESDLVIFSADKKAAFGDINSQSLVDTNDKYGVSFTSKDAASATLNLLLFTCPFGDTGSTDYENFKKYNAHLKNAHNKTICMICASHKKAFPSELRVYTPNQLRVHQSKGDSEGFKGHPMCGFCSGQRFYSDDELNIHMRDRHERCHICDQENSASPRYFKDYDQLFEHFKFEHYICTVQSCLDSKFVVFRDDLDLQAHILKEHGSILRNGNNNAALKGRKYQSQLSTFSRPSSRNSSTSDFGGVSSKKEDPENSQEVKRLRMEERARHYLNYSHTDFEQFLAINESYKNKMITAQDVYSAYENLFKSPQANTALLLYDFSELFPKNTKFHQDLRAIYDSEQKKQDRFTNFPSLSNSSPSLLAGNVVSGSWGRNGGTNKAARQFNFPALKKPSSPQPLLVPQKVSYSKPKSATPKVAPSLTTRKSSSTESFYKPTYLDNKKASVAPSAAVDREKFPPLPKSQAKKFRAPLVNQPDIPDPSQWGKTPPSSRQNSETSNRDTTDTASPIHVKKGKQKQLLFHIGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DH98</accession>
    <name>C5DH98_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0E02508p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0E02508g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553596.1">
        <property type="nucleotide sequence ID" value="XM_002553550.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DH98"/>
    <dbReference type="FunCoup" id="C5DH98">
        <property type="interactions" value="54"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DH98"/>
    <dbReference type="GeneID" id="8291738"/>
    <dbReference type="KEGG" id="lth:KLTH0E02508g"/>
    <dbReference type="eggNOG" id="KOG2961">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056221_3_2_1"/>
    <dbReference type="InParanoid" id="C5DH98"/>
    <dbReference type="OMA" id="MLMANMM"/>
    <dbReference type="OrthoDB" id="198652at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008962">
        <property type="term" value="F:phosphatidylglycerophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032049">
        <property type="term" value="P:cardiolipin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000165">
        <property type="entry name" value="HAD superfamily phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027706">
        <property type="entry name" value="PGP_Pase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010021">
        <property type="entry name" value="PGPP1/Gep4"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01668">
        <property type="entry name" value="YqeG_hyp_ppase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288">
        <property type="entry name" value="4-NITROPHENYLPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288:SF25">
        <property type="entry name" value="PHOSPHATIDYLGLYCEROPHOSPHATASE GEP4, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09419">
        <property type="entry name" value="PGP_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR23159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="191" mass="21012" checksum="17AE09D7D4F310D8" modified="2009-07-28" version="1">MNVSATLNVLRLLQNPGLCIPQLTVGNFGQIPVPIGPSIKAVVLDKDNCFAYPHSNEVWPDYEACKGKTWVELKKAYPGASLLIVSNSAGSSDDKDLKQARLLEETTGVPVLRHKVKKPGCRDEILSYFSGKKITNDPSEIAVVGDRLFTDIMMANLMGSYGVWVRDGVKPSKNPIVHFEKLLFKWLTPGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHP9</accession>
    <name>C5DHP9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">KLTH0E06094p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0E06094g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PIGG/PIGN/PIGO family. PIGO subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553747.1">
        <property type="nucleotide sequence ID" value="XM_002553701.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHP9"/>
    <dbReference type="FunCoup" id="C5DHP9">
        <property type="interactions" value="689"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHP9"/>
    <dbReference type="GeneID" id="8291900"/>
    <dbReference type="KEGG" id="lth:KLTH0E06094g"/>
    <dbReference type="eggNOG" id="KOG2126">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004298_1_0_1"/>
    <dbReference type="InParanoid" id="C5DHP9"/>
    <dbReference type="OMA" id="EDEYVIM"/>
    <dbReference type="OrthoDB" id="272139at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051377">
        <property type="term" value="F:mannose-ethanolamine phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16023">
        <property type="entry name" value="GPI_EPT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037675">
        <property type="entry name" value="PIG-O_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039524">
        <property type="entry name" value="PIGO/GPI13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23071:SF1">
        <property type="entry name" value="GPI ETHANOLAMINE PHOSPHATE TRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23071">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL GLYCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="450"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="486"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="517"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="557"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="585"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="686"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="717"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="760"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="800"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="904"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="937"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="980"/>
            <end position="1002"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR23310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1016" mass="114435" checksum="3F6F7DB3DDF99132" modified="2009-07-28" version="1">MPPIDSTNEDYVKKNMLSSSIEDKRLFKSRIQRFKTTHRLYIYLMGSLAVLQIIAIAFFARGFLLTRTVLENVATGADHEARFSKCVILVIDALRFDFSIPVDPADNAFNSYFHNNLDVLHTSFNNATSETHSSLLLKFIADPPTTTLQRLKGLTTGSLPTFIDAGSNFNGAVIEEDNLIKQMFLNNQSVLFAGDDTWDNLFNPFLSPASQPFESLNVWDLDTVDNGVIRFFEDNLFAKSEKSEEWDVLIGHMLGIDHVGHKYGPNHFTMKEKQLQANEFIKRICESIDDDTLLVVMGDHGMDHTGNHGGDSKSELEAALWLHSKRPNMWSHLPEQFYDISALGENYRQVNQIDLVPTLSLLLGLPIPFNNLGWPIQEVAATQEQLRSYSKIALDQIKAYKDISRVITAAEKNERLNELLNESLEDILQASEYQKIFLESCKDLWARFDYFSIATGIILLTASLVLLITITKLIPSIVVGQMVTELVPSITIMALVSNVCFIGIFHVLHQPKFLENWIWCSLLATAVGIIVGCFIPLFDRYNFAWITVKFIEDLSDYWSRIAAMCVTLHALLFTSNSFTIWEDKIVSFLLATLGMLTLYEFVFLPKRKLTTALLSAAMGEKRGSSSSNASSSSETESSPLGKFARFVGSYHSIVLIACTRISSMITICREEQGEYCTPTFTTANNYSPWCLSLCLVLVAYLPSLIKGYYNVCSSYQAAAPIWIGQFLKFVLLGNFVYWTVCAMENTVPTWSWDVKIFKLTLSRIIAGFSLVAANIGWLMGPLCVKINIHNNDVKSQQATILGYANAYGSQYFLLILNFFMCIMLFNKPMAQLSLSLMCTQLLSMLEIFDLLKLKENLIGPVSMGLLAYQQFFSTGHQATIPAVQWDIGFILTERITFPFTHLGIVLNTFGPHIIVALSVALLTLWKQPPSVLKPQTLLARIVSNCGMLLIYNTVLCLSSFVWVTHFRRHLMVWKIFCPRFMFASLSLIVTQVIVTLVTIAFASGRLIRQINTIFPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHQ7</accession>
    <name>C5DHQ7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">KLTH0E06292p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0E06292g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endomembrane system</location>
            <topology evidence="8">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 9">Membrane</location>
            <topology evidence="1 9">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553755.1">
        <property type="nucleotide sequence ID" value="XM_002553709.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHQ7"/>
    <dbReference type="FunCoup" id="C5DHQ7">
        <property type="interactions" value="1001"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHQ7"/>
    <dbReference type="GeneID" id="8291909"/>
    <dbReference type="KEGG" id="lth:KLTH0E06292g"/>
    <dbReference type="eggNOG" id="KOG1692">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066963_4_0_1"/>
    <dbReference type="InParanoid" id="C5DHQ7"/>
    <dbReference type="OMA" id="MQVRDRN"/>
    <dbReference type="OrthoDB" id="62956at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015720">
        <property type="entry name" value="Emp24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036598">
        <property type="entry name" value="GOLD_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
        <property type="entry name" value="TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101576">
        <property type="entry name" value="Supernatant protein factor (SPF), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002948743" evidence="10">
        <location>
            <begin position="20"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="11">
        <location>
            <begin position="29"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR23318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="202" mass="23201" checksum="69062B5ACCF26A84" modified="2009-07-28" version="1">MKFFICSLVALMWCSLCGAHNVLLPAYGERCFFETLSKNDELAVSFQFGDRAPDSTEQLKGDFIMYSSDRRDALRTFRDVSHGDVRLRVPHGGKFEYCFVNINSGATTKDVTFNIEGTIFVDPNDPKSDSLDGAVKQLSRLTKELKNEQSYIVIRERTHRNTAESTNDRVKWWSVFQLGVVIASSLFQVYYLRRFFEVTSYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHR8</accession>
    <name>C5DHR8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Vitamin B6 transporter TPN1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Transport of pyridoxine protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0E06600g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Thiamine-regulated, high affinity import carrier of pyridoxine, pyridoxal and pyridoxamine.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the purine-cytosine permease (2.A.39) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553766.1">
        <property type="nucleotide sequence ID" value="XM_002553720.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHR8"/>
    <dbReference type="FunCoup" id="C5DHR8">
        <property type="interactions" value="62"/>
    </dbReference>
    <dbReference type="GeneID" id="8291921"/>
    <dbReference type="KEGG" id="lth:KLTH0E06600g"/>
    <dbReference type="eggNOG" id="ENOG502QR29">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026016_2_1_1"/>
    <dbReference type="InParanoid" id="C5DHR8"/>
    <dbReference type="OMA" id="YERWAWV"/>
    <dbReference type="OrthoDB" id="5428495at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.4160.10:FF:000007">
        <property type="entry name" value="Pyridoxine transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4160.10">
        <property type="entry name" value="Hydantoin permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001248">
        <property type="entry name" value="Pur-cyt_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026030">
        <property type="entry name" value="Pur-cyt_permease_Fcy2/21/22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31806">
        <property type="entry name" value="PURINE-CYTOSINE PERMEASE FCY2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31806:SF17">
        <property type="entry name" value="VITAMIN B6 TRANSPORTER TPN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02133">
        <property type="entry name" value="Transp_cyt_pur"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002744">
        <property type="entry name" value="Pur-cyt_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="64"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="98"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="176"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="202"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="244"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="279"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="332"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="372"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="397"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="478"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="516"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00056781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00073028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00083325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR002744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR23329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="542" mass="60533" checksum="E8D83C27CA625478" modified="2009-07-28" version="1">MGAEKVEDIVFEEIEKPKTDEGQGVFAKVLTKISAASKRLDAMGVEMRGIQRIPGYERGTSKQFLSVAGLWLSAAGGLSSMSSYFLGPLLFELTFRQSLVSGLISMALGCAVAGYLSTMGPQSGCRQMVTARYLFGWYFVKFVSLASIIGVMGWSIVNCVVGGQMLAAVSDSKIPLWVGIIIVTAVSFFVAIFGIKQVLRVETFLSVPVLTCFTLLYIAASDKFHLLDSFQNGDVDRLTIKGNWLSFFSLCYSITSTWGSITADYYILFPEDTAHYKVFLLTFFGTLLPTTFVGVLGLILGSCALSYEPWNEAYGKYGMGGLLYSGFQRWNGFGKFCVVVLILSLISNNIINTYSAAFNLQLSSIHMARVPRWIWAILCTIFYLVCALVGRNHFSDILGNFLPMIGYWISMYFIILLEENLIFRSHFLHLYTKEFPPQPVEERLSRLMLRDIKNKKVSARKCSYNWEMWNNERVLTHGYAATFAFLVGVGGVALSMAQAYWIGPIARHFGEYGGDLGMWVSMGFSGVVYPALRYLELRKFGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHZ3</accession>
    <name>C5DHZ3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0E08382p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0E08382g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the EF-1-beta/EF-1-delta family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553841.1">
        <property type="nucleotide sequence ID" value="XM_002553795.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHZ3"/>
    <dbReference type="FunCoup" id="C5DHZ3">
        <property type="interactions" value="823"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHZ3"/>
    <dbReference type="GeneID" id="8292002"/>
    <dbReference type="KEGG" id="lth:KLTH0E08382g"/>
    <dbReference type="eggNOG" id="KOG1668">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050172_0_2_1"/>
    <dbReference type="InParanoid" id="C5DHZ3"/>
    <dbReference type="OMA" id="YRWYKHI"/>
    <dbReference type="OrthoDB" id="331763at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005853">
        <property type="term" value="C:eukaryotic translation elongation factor 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00292">
        <property type="entry name" value="EF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.60:FF:000001">
        <property type="entry name" value="Elongation factor 1-beta 1 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053836">
        <property type="entry name" value="Arc1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036219">
        <property type="entry name" value="eEF-1beta-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018940">
        <property type="entry name" value="EF-1_beta_acid_region_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049720">
        <property type="entry name" value="EF1B_bsu/dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014038">
        <property type="entry name" value="EF1B_bsu/dsu_GNE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014717">
        <property type="entry name" value="Transl_elong_EF1B/ribsomal_bS6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001326">
        <property type="entry name" value="Transl_elong_EF1B_B/D_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11595">
        <property type="entry name" value="EF-HAND AND COILED-COIL DOMAIN-CONTAINING FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11595:SF21">
        <property type="entry name" value="ELONGATION FACTOR 1-BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21972">
        <property type="entry name" value="Arc1p_N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10587">
        <property type="entry name" value="EF-1_beta_acid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00736">
        <property type="entry name" value="EF1_GNE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01182">
        <property type="entry name" value="EF-1_beta_acid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00888">
        <property type="entry name" value="EF1_GNE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54984">
        <property type="entry name" value="eEF-1beta-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00824">
        <property type="entry name" value="EF1BD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00825">
        <property type="entry name" value="EF1BD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="3 4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Elongation factor 1 beta central acidic region eukaryote" evidence="6">
        <location>
            <begin position="86"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="Translation elongation factor EF1B beta/delta subunit guanine nucleotide exchange" evidence="5">
        <location>
            <begin position="122"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR23404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="208" mass="22944" checksum="BCC2A5B2632DC010" modified="2009-07-28" version="1">MSFTDFSKIETLKELNTFLADKSYIEGTSASQADVSVYKAFQQAYPDFARWFNHIAVKSGQFDSLPAAKAPAAPAEAEDDDDDVDLFGSSDEEVDEEAEKLKAKRLAEYNAKKAAKPKPAAKSIVTLDVKPWDDETDLEEMLAGVKSIEMDGLVWGAHQWIPLGFGIKKLQINLVVEDAKVSLEDLQVAIEEDEDHVQSTDIAAMSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5DI63</accession>
    <name>C5DI63_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0E10010p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0E10010g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553911.1">
        <property type="nucleotide sequence ID" value="XM_002553865.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DI63"/>
    <dbReference type="FunCoup" id="C5DI63">
        <property type="interactions" value="98"/>
    </dbReference>
    <dbReference type="GeneID" id="8292075"/>
    <dbReference type="KEGG" id="lth:KLTH0E10010g"/>
    <dbReference type="eggNOG" id="ENOG502S5PF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_667626_0_0_1"/>
    <dbReference type="InParanoid" id="C5DI63"/>
    <dbReference type="OMA" id="EIERHYP"/>
    <dbReference type="OrthoDB" id="3219396at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="12"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="195"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="251"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="291"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="410"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="291"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="318"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="419"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR23474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="450" mass="50870" checksum="F8C9C97EB2884770" modified="2009-07-28" version="1">MSARDPRTATPSRSLTDLPLDVIMEILFYLPFEDLENVSKTCRTLRVLSNESITYRRTVKDRCVASEWTKRLLFDFLHAIDKKDGLLGFISSERISIVNSLQELQSRFELGNRVPLLPPREMTPLQNSAASDENKVKVSETPCSESSGKQELKPNSFEIPKARLRTGKRDAASLDKEGMAYLQILQGFHRIATNSHKRFGRRNREFARRNPNQESAVPQTPDKEPIASATLTPIQVQSLANEDTTAIDVLKSDENSPDSSHHSRTTSSIFSDTPRLSDLGWSYSEEFKSLESNSDSECGSSDSSSSSKYLRQLQRSNRVSDKKNLYEKLNSRTRKEHEITNNKISEKGSSEKLRPGPTSSKGRAFSQGYLVELERCNTPAVRTQQTAAETTKVSQEFLSRYQEHLVIGTKSSPEVPTQRRSKTKKLGHAPHRRKLIASVTEDNRICYEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>C5DI74</accession>
    <name>C5DI74_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Adenylate kinase</fullName>
            <ecNumber evidence="6">2.7.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">ATP-AMP transphosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">ATP:AMP phosphotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Adenylate kinase cytosolic and mitochondrial</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Adenylate monophosphate kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ADK1</name>
        <name evidence="9" type="ordered locus">KLTH0E10274g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism. Adenylate kinase activity is critical for regulation of the phosphate utilization and the AMP de novo biosynthesis pathways.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>AMP + ATP = 2 ADP</text>
            <dbReference type="Rhea" id="RHEA:12973"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.3"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
            <location evidence="6">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Mitochondrion intermembrane space</location>
        </subcellularLocation>
        <text evidence="6">Predominantly mitochondrial.</text>
    </comment>
    <comment type="domain">
        <text evidence="6">Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon ATP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent ATP hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the adenylate kinase family. AK2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.3" evidence="6"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553922.1">
        <property type="nucleotide sequence ID" value="XM_002553876.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DI74"/>
    <dbReference type="FunCoup" id="C5DI74">
        <property type="interactions" value="879"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DI74"/>
    <dbReference type="GeneID" id="8292087"/>
    <dbReference type="KEGG" id="lth:KLTH0E10274g"/>
    <dbReference type="eggNOG" id="KOG3078">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032354_1_0_1"/>
    <dbReference type="InParanoid" id="C5DI74"/>
    <dbReference type="OMA" id="VYHEQTA"/>
    <dbReference type="OrthoDB" id="439792at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:AMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006172">
        <property type="term" value="P:ADP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046033">
        <property type="term" value="P:AMP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046034">
        <property type="term" value="P:ATP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01428">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000106">
        <property type="entry name" value="Adenylate kinase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00235">
        <property type="entry name" value="Adenylate_kinase_Adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03168">
        <property type="entry name" value="Adenylate_kinase_AK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006259">
        <property type="entry name" value="Adenyl_kin_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000850">
        <property type="entry name" value="Adenylat/UMP-CMP_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033690">
        <property type="entry name" value="Adenylat_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007862">
        <property type="entry name" value="Adenylate_kinase_lid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028587">
        <property type="entry name" value="AK2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01351">
        <property type="entry name" value="adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001380">
        <property type="entry name" value="PRK00279.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001381">
        <property type="entry name" value="PRK00279.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011100">
        <property type="entry name" value="PRK14527.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23359">
        <property type="entry name" value="NUCLEOTIDE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00406">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05191">
        <property type="entry name" value="ADK_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00094">
        <property type="entry name" value="ADENYLTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00113">
        <property type="entry name" value="ADENYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Adenylate kinase active site lid" evidence="8">
        <location>
            <begin position="139"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="NMPbind" evidence="6">
        <location>
            <begin position="41"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="LID" evidence="6">
        <location>
            <begin position="138"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="21"/>
            <end position="26"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="68"/>
            <end position="70"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="97"/>
            <end position="100"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="148"/>
            <end position="149"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF05191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR23485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="227" mass="25175" checksum="937CCFF705D5C315" modified="2009-07-28" version="1">MAQDLKHVPESIRMVLIGPPGSGKGTQAPNLKERFCACHLATGDMLRSQVAQKTELGLEAKKIMDQGGLVSDEIMVSMIKHELITNPECKSGFILDGFPRTIPQAEKLDQMLAEQGRPLEKAVELKIDDELLVSRITGRLVHPSSGRSYHKLFNPPKEDMKDDITGEPLVQRSDDNADALKKRLGAYHEQTEPIVDFYKKTGIWSGVDASQSPQTVWTDILKCLGKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>C5DID1</accession>
    <name>C5DID1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0E11572p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0E11572g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein biosynthesis; polypeptide chain elongation.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553979.1">
        <property type="nucleotide sequence ID" value="XM_002553933.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DID1"/>
    <dbReference type="FunCoup" id="C5DID1">
        <property type="interactions" value="986"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DID1"/>
    <dbReference type="GeneID" id="8292146"/>
    <dbReference type="KEGG" id="lth:KLTH0E11572g"/>
    <dbReference type="eggNOG" id="KOG0867">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1627">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011226_3_0_1"/>
    <dbReference type="InParanoid" id="C5DID1"/>
    <dbReference type="OMA" id="PFVMEEC"/>
    <dbReference type="OrthoDB" id="249703at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03181">
        <property type="entry name" value="GST_C_EF1Bgamma_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03044">
        <property type="entry name" value="GST_N_EF1Bgamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000006">
        <property type="entry name" value="Elongation factor 1 gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000142">
        <property type="entry name" value="Elongation factor 1 gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1010:FF:000001">
        <property type="entry name" value="Elongation factor 1-gamma 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1010">
        <property type="entry name" value="Translation elongation factor EF1B, gamma chain, conserved domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050802">
        <property type="entry name" value="EF-GSTs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001662">
        <property type="entry name" value="EF1B_G_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036433">
        <property type="entry name" value="EF1B_G_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43986">
        <property type="entry name" value="ELONGATION FACTOR 1-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43986:SF1">
        <property type="entry name" value="ELONGATION FACTOR 1-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00647">
        <property type="entry name" value="EF1G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01183">
        <property type="entry name" value="EF1G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89942">
        <property type="entry name" value="eEF1-gamma domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50040">
        <property type="entry name" value="EF1G_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="3 4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="7">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="8">
        <location>
            <begin position="87"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="EF-1-gamma C-terminal" evidence="6">
        <location>
            <begin position="256"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="213"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="220"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR23542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="417" mass="47834" checksum="ACF87753BD1A579B" modified="2009-07-28" version="1">MSLGTLYGNFRIRTWVPQAIIKKFGLDIKIIAPEDNEQQFAKDFPLKKVPGFLGPEGFKLTETMAVMYYLTKLIPDEEAKRELLGANAEEQAQVIRWLSFANSELLIELVNVFKPLLGMSPFIKPLFDAAVAKVDQITAVFEERLIEHTYLASEDITLADLFATTVFARGFQHCLGSEWRAAHPAITRWYNTVKASDYLEEFFVDFKPCDKPLQPPQNNKKKEAKKETKKEAKKPSAEKPKAAAEAEKPAEQPKKPKHPLELLGKASFPLEDWKRKYSNEDTRPVALPWFWEHYNPEEYSIWRVDFKYNDELTLCFMSNNQVGGFMERLSASVKYMFGCIVVYGEDRNNGIVGAVMLRGQDYVPAFDVAPDWTSYDYAKLDPSKEEDKEFINTMWAWDKPVVINGEPREISDGKVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DID3</accession>
    <name>C5DID3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0E11616p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0E11616g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the ANP1/MMN9/VAN1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553981.1">
        <property type="nucleotide sequence ID" value="XM_002553935.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DID3"/>
    <dbReference type="FunCoup" id="C5DID3">
        <property type="interactions" value="128"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DID3"/>
    <dbReference type="GeneID" id="8292148"/>
    <dbReference type="KEGG" id="lth:KLTH0E11616g"/>
    <dbReference type="eggNOG" id="ENOG502QRPX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017872_4_0_1"/>
    <dbReference type="InParanoid" id="C5DID3"/>
    <dbReference type="OMA" id="IPKTREG"/>
    <dbReference type="OrthoDB" id="2405412at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000136">
        <property type="term" value="C:mannan polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000009">
        <property type="term" value="F:alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000032">
        <property type="term" value="P:cell wall mannoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000017">
        <property type="entry name" value="Mannan polymerase II complex ANP1 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052086">
        <property type="entry name" value="Mannan_Polymerase_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43083:SF6">
        <property type="entry name" value="MANNAN POLYMERASE COMPLEXES SUBUNIT MNN9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43083">
        <property type="entry name" value="MANNAN POLYMERASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03452">
        <property type="entry name" value="Anp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR23544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="394" mass="45173" checksum="73D7BBD22C1661E3" modified="2009-07-28" version="1">MSLAMWSYRLRKTPLVALLVPLVLVGVLLWVFLGNEESLLGLDGSSISTHKWAHESEKTFYFPYSHMFKTPKYSYKKKSNWLFSDTVEDNIPAGHISHYDLNKLTSTDKAFANKEQVLILTPMQTFHDQYWDNLLQLTYPRDLIELGFIIPRTATGDTALRKLEAAVKRTQLGKKDQRFSKITILRQDSQSFDSLQEKERHALKVQKERRAAMALARNELLFSTIGPHTSWILWLDADIIETPPSLIQDMTAHNKPVLAANVYQKYMNEETKKIAERPYDFNNWADSEKALQMAAGMADDEVLFEGYAELPTYRTLMAHMYDASGSQAAEMSLDGVGGGCTLVKAEVHRDGAMFPSFPFYHLIETEGFAKMARRLEYEIFGLPNYLVYHIEEFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIG8</accession>
    <name>C5DIG8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Derlin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0E12408g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">May be involved in the degradation of misfolded endoplasmic reticulum (ER) luminal proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Endoplasmic reticulum membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the derlin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554016.1">
        <property type="nucleotide sequence ID" value="XM_002553970.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIG8"/>
    <dbReference type="FunCoup" id="C5DIG8">
        <property type="interactions" value="100"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIG8"/>
    <dbReference type="GeneID" id="8292184"/>
    <dbReference type="KEGG" id="lth:KLTH0E12408g"/>
    <dbReference type="eggNOG" id="KOG0858">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1256224_0_0_1"/>
    <dbReference type="InParanoid" id="C5DIG8"/>
    <dbReference type="OMA" id="FKKGQYE"/>
    <dbReference type="OrthoDB" id="1716531at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007599">
        <property type="entry name" value="DER1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11009">
        <property type="entry name" value="DER1-LIKE PROTEIN, DERLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04511">
        <property type="entry name" value="DER1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="94"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR23579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="220" mass="25409" checksum="9D0D4C3C75F1E993" modified="2009-07-28" version="1">MDILLVNLVGDIPVVTKTWVGGVLAMSVLSSTTIIDPTKFIYNYDLVFKKGQFTRVLYSLFDYGEFSLIYFVQLMFLTQELAALEKSIPQRSQFLWMIFVMGVLTIIFSKWIQPFEPLASVLHKNLTYYKLRKDVQLQQGPQRGLAASPLMVRVCFDIVLVCHFGHTIRGILLRYLAGHLYYFLEQIASKVYGINLCKPPHKWFAARERGNEEEAEQLQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>C5DII5</accession>
    <name>C5DII5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">KLTH0E12782p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0E12782g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>5-methyl-UTP(out) + UTP(in) = 5-methyl-UTP(in) + UTP(out)</text>
            <dbReference type="Rhea" id="RHEA:73523"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:63527"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554033.1">
        <property type="nucleotide sequence ID" value="XM_002553987.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DII5"/>
    <dbReference type="FunCoup" id="C5DII5">
        <property type="interactions" value="478"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DII5"/>
    <dbReference type="GeneID" id="8292201"/>
    <dbReference type="KEGG" id="lth:KLTH0E12782g"/>
    <dbReference type="eggNOG" id="KOG0757">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015166_6_0_1"/>
    <dbReference type="InParanoid" id="C5DII5"/>
    <dbReference type="OMA" id="WVMYEQM"/>
    <dbReference type="OrthoDB" id="269120at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015218">
        <property type="term" value="F:pyrimidine nucleotide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990519">
        <property type="term" value="P:pyrimidine nucleotide import into mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000079">
        <property type="entry name" value="Mitochondrial carrier protein RIM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000088">
        <property type="entry name" value="Mitochondrial carrier protein RIM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049562">
        <property type="entry name" value="SLC25A33/36-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45829">
        <property type="entry name" value="MITOCHONDRIAL CARRIER PROTEIN RIM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45829:SF4">
        <property type="entry name" value="MITOCHONDRIAL CARRIER PROTEIN RIM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="51"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="168"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="279"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00093195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR23596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="372" mass="41251" checksum="EC7AD8A14074E407" modified="2009-07-28" version="1">MPKKTLEQLEQEARESVPYLGSDEDASNLKNVNFASKRGATAVAAPSSSEVKPWVHFVAGGLGGMAGAVVTCPFDVVKTRLQSDVFQAAYKSANPGPHKSSNFVMSGARHFRETFGIISNVYKQEGFRSLFKGLGPNLVGVIPARSINFFTYGTTKQIYSRAFNNGEEAPWIHLISAATAGWATSTATNPIWLIKTRLQLDKAGHTKQYKNSWDCLKHILQKEGFFGLYKGLSASYLGSVEGILQWLLYEQMKQMIKMRSIEKFGHISEGEKSTSEQIKEWCQRSGSAGLAKFMASIVTYPHEVVRTRLRQAPLENGKLKYTGLIQSFRVIIKEEGLASMYGGLTPHLLRTVPNSIIMFGTWELVIKLLSDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DII6</accession>
    <name>C5DII6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0E12804p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0E12804g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="4">Component of the large ribosomal subunit (LSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL21 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554034.1">
        <property type="nucleotide sequence ID" value="XM_002553988.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DII6"/>
    <dbReference type="FunCoup" id="C5DII6">
        <property type="interactions" value="848"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DII6"/>
    <dbReference type="GeneID" id="8292202"/>
    <dbReference type="KEGG" id="lth:KLTH0E12804g"/>
    <dbReference type="eggNOG" id="KOG1732">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103610_0_1_1"/>
    <dbReference type="InParanoid" id="C5DII6"/>
    <dbReference type="OMA" id="HVQASRC"/>
    <dbReference type="OrthoDB" id="1539250at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.70:FF:000001">
        <property type="entry name" value="60S ribosomal protein L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.250.3260:FF:000001">
        <property type="entry name" value="60S ribosomal protein L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.70">
        <property type="entry name" value="Ribosomal protein L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001147">
        <property type="entry name" value="Ribosomal_eL21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018259">
        <property type="entry name" value="Ribosomal_eL21_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036948">
        <property type="entry name" value="Ribosomal_eL21_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20981">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01157">
        <property type="entry name" value="Ribosomal_L21e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01171">
        <property type="entry name" value="RIBOSOMAL_L21E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="107"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00064933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR23597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="147" mass="16561" checksum="BDED1943CD14558F" modified="2009-07-28" version="1">MFQRDFRKHGAIPLSTYLKVYKVGDIVDIKANGSIQKGMPHKFYQGKTGVVYNVTKSAVGVIINKVVGNRYIEKRVSLRVEHIKHSKCRQEFLDRVKSNAAKRREAKAQGVAVQLKRQPAQPRGSRVVSTEGNIPKTLAPVPYETFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIN9</accession>
    <name>C5DIN9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 16">E3 ubiquitin-protein ligase listerin</fullName>
            <ecNumber evidence="5 16">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">RING-type E3 ubiquitin transferase listerin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ordered locus">KLTH0E13992g</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">E3 ubiquitin-protein ligase component of the ribosome quality control complex (RQC), a ribosome-associated complex that mediates ubiquitination and extraction of incompletely synthesized nascent chains for proteasomal degradation. Mediates ubiquitination of proteins derived from mRNAs lacking stop codons (non-stop proteins) and other translation arrest products induced by poly-lysine sequences and tandem rare codons. Ubiquitination leads to CDC48 recruitment for extraction and degradation of the incomplete translation product. May indirectly play a role in chromatin function and transcription.</text>
    </comment>
    <comment type="function">
        <text evidence="16">E3 ubiquitin-protein ligase. Component of the ribosome quality control complex (RQC), a ribosome-associated complex that mediates ubiquitination and extraction of incompletely synthesized nascent chains for proteasomal degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 16">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Component of the ribosome quality control complex (RQC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 16">Belongs to the LTN1 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5 16"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554087.1">
        <property type="nucleotide sequence ID" value="XM_002554041.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DIN9">
        <property type="interactions" value="630"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIN9"/>
    <dbReference type="GeneID" id="8292256"/>
    <dbReference type="KEGG" id="lth:KLTH0E13992g"/>
    <dbReference type="eggNOG" id="KOG0803">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_262564_0_0_1"/>
    <dbReference type="InParanoid" id="C5DIN9"/>
    <dbReference type="OMA" id="NRFHGAC"/>
    <dbReference type="OrthoDB" id="6108at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990112">
        <property type="term" value="C:RQC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990116">
        <property type="term" value="P:ribosome-associated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16491">
        <property type="entry name" value="RING-CH-C4HC3_LTN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000038">
        <property type="entry name" value="E3 ubiquitin-protein ligase listerin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039795">
        <property type="entry name" value="LTN1/Rkr1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054477">
        <property type="entry name" value="LTN1_E3_ligase_6th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054476">
        <property type="entry name" value="Ltn1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054478">
        <property type="entry name" value="LTN1_UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039804">
        <property type="entry name" value="RING-CH-C4HC3_LTN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12389:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE LISTERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12389">
        <property type="entry name" value="ZINC FINGER PROTEIN 294"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22958">
        <property type="entry name" value="Ltn1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22999">
        <property type="entry name" value="LTN1_E3_ligase_6th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23009">
        <property type="entry name" value="UBC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01197">
        <property type="entry name" value="FANCL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 16" id="KW-0808">Transferase</keyword>
    <keyword evidence="12 16" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="13 16" id="KW-0862">Zinc</keyword>
    <keyword evidence="11 15 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="18">
        <location>
            <begin position="1496"/>
            <end position="1543"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="1381"/>
            <end position="1415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00055150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU367090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAR23650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1550" mass="176988" checksum="681982B08F553585" modified="2009-07-28" version="1">MSFGNDLSDFGLGNNGVKITVNYFDGLTDQSLIGALGSVELQLIFKSLLKRDETTKERAVNELLELMKDPNGNRQVFEDECFALCWSQIYAKLITNESRILRISAHTVTTSLIKIMGKKGAKFLKDFVPFLLSGSYDHDATVAKACANSTLECFGNDVTKVNALWTLFLPQILNFVKELVVVESQASLSDERYVSKQESELKYIRVVTSAICVLIRLSKENANENLLRNPLYQEIISNSDLWKLLSFKNMHNLKTCESLLLLAKTLWDLGFLQSSKDVLKPISKRLLKAISQISSKNVFSVSPIFPDILSVMVCLNNYKNGKFLSWDKSSREKIQTFLLLGPGNADPSYYQLLLSFYENISPYLDLDYESDWLPIWRKDLQYEKERRGVPARKEALLNQFWINYLKFTSEAPLQYREIANSSIVKDIIEFLNEKSLTQSKSLINTFSKALPFEILKSELISILPVGTNKQHENKCYIENLLDIAFEKEDNKPFLKSVAQLVISSLEERPAEEMYFILCFFDRLTETDVSQLNDEVFSFLKMLPNYLSPKFYDAPFKFLLDFSKSRKLISSNQSGLCDALDRFLQSFLLLDIPRTVKVSQLTKLDKELFEKLLDRSEALQQFRSLVINSFDFSNPDFYQAHFLSENNIIKVYELAVGHNQLDQFCSNCVVIQPSLYDYLLQKSNLIADALFEDFGTVTSQLKNKVLALAESDEALGEKAAHAVLKHVRSKFNELSEEFFLYVEKLLSISPKAKDVIFVGDLYADLDKSVPFLSHQLALSSSLGLNVHFLDTSSENLNRDLCLPSLRYAEFIDALLKRNQSFQTENNLLFLTLASELASDYNFFSAEPLDHLQDLECVVFKDSRYNFDFHTIVDSILNPGNSNISVLKTLNTLENISDVAIVYNCRVLHKMLGNAVDLSSPSDAKELAAIDKFVTERLRAKGSSSREFLMAGTLLLSVSRSDYFTESLTRLRTFLASELIGAKGAEMVNRAPKVTILLNDMLVSEGTRLHNNSYVPIAHQRLRMVLTEIGKWFDSDLCYDESFILLRLSVLKFLISLLKHSSSVSLGSLLFDLSVRVLKDCFEVLSLDEMLFSQELSLFSVYLYQQLIELGKNNALDAGLWQETEVGILEIAVEVFMMKTDLQKANHFTFLFYRLLVQVFSDASVEHFLPLTNNLLEKFLSSPDLNIDQTRLIVLVLRKSILAKQQDLLIEYELSNASKSAEDEPNDTFQFPSLLLTAIDQKMPEEYLEYENEDQFLKYLWCCYLALSYLKDISYNLRQSFISQLKDQGLVTKLYDFIADQLNLEDKAWIPTDESTIETYDVAAPDSSSTKSALLQECKELMLHLLYVLFKNMGSMTSSWWLNLKDRSLQAKVEKFVTMHVSKLLIRQELKEVSEKVKKLTSQDESLTIRLNNATNEIKAGYLVDEQKLEISLKIPSNYPLSNIQVHGNSRVGINEQKWKSWILSAQRVIVGMNGSVMDSLELFTKNVNLHFSGFEECAICYSILHAVDRKLPSKVCPTCNNRFHGACLYKWFRSSGNNTCPLCRSEIPFRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-04-02" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIQ8</accession>
    <name>C5DIQ8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0E14432p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0E14432g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554106.1">
        <property type="nucleotide sequence ID" value="XM_002554060.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIQ8"/>
    <dbReference type="GeneID" id="8292276"/>
    <dbReference type="KEGG" id="lth:KLTH0E14432g"/>
    <dbReference type="eggNOG" id="ENOG502S1A5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_625521_0_0_1"/>
    <dbReference type="InParanoid" id="C5DIQ8"/>
    <dbReference type="OMA" id="LYYPMAT"/>
    <dbReference type="OrthoDB" id="4096434at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="219"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="405"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="140"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="235"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="252"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="287"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="422"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="448" mass="48775" checksum="9C157C06ECD58AD8" modified="2009-07-28" version="1">MLYQGVEDHSAFDSRMLMMPKRGDAGGGGSGAAAVRAASAQPGDASRTHSGAHGGAFGTTGGALDAVPDTELDPLVNLYYPMATAVPPPAHNDISYNSSVLSFSAASPSSLYDMDFYTSNYSGRPEAGRLSNEGRAGSVAGAQGPTAPAGQRDSFLSYSVPTEHSQIRTQQVAEQGPLYTASPLYGASATAFGSINGSAWAGPLGTNLNREVRRSSSLVMPGVPMPNVNTDLNINTASASNHNPNNTNNNYEYEDAEEDGNDEAEEDADNSDSDNQPHLQRQRRFSSSRTPSTGAIATSFSQGSKKVSDSRLSAQGLAEVLNLESAEEALRRERFILDIFERELQYPLGYKTWVRDTSKEYRTQLLDQLHRRVSQTYPEYDKPVLETIIRRATYYMMQSRLRRERRAKAKVKRDEERRSRGSRGSENGHGSQFELPHFGGPVDPGFLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIW4</accession>
    <name>C5DIW4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">L2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">RP2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">YL2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0E15686g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="4">Component of the large ribosomal subunit (LSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes). uL4 is associated with the polypeptide exit tunnel. uL4 interacts with its chaperone ACL4 and the nuclear import receptor KAP104.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554162.1">
        <property type="nucleotide sequence ID" value="XM_002554116.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIW4"/>
    <dbReference type="FunCoup" id="C5DIW4">
        <property type="interactions" value="1684"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIW4"/>
    <dbReference type="GeneID" id="8292333"/>
    <dbReference type="KEGG" id="lth:KLTH0E15686g"/>
    <dbReference type="eggNOG" id="KOG1475">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026535_4_0_1"/>
    <dbReference type="InParanoid" id="C5DIW4"/>
    <dbReference type="OMA" id="ALYGTWR"/>
    <dbReference type="OrthoDB" id="10259785at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1370.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025755">
        <property type="entry name" value="Ribos_uL4_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002136">
        <property type="entry name" value="Ribosomal_uL4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023574">
        <property type="entry name" value="Ribosomal_uL4_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013000">
        <property type="entry name" value="Ribosomal_uL4_euk/arc_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045240">
        <property type="entry name" value="Ribosomal_uL4_euk/arch"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19431">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14374">
        <property type="entry name" value="Ribos_L4_asso_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00573">
        <property type="entry name" value="Ribosomal_L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52166">
        <property type="entry name" value="Ribosomal protein L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00939">
        <property type="entry name" value="RIBOSOMAL_L1E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL4 C-terminal" evidence="9">
        <location>
            <begin position="273"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="344"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00064557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00077544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00079326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00080732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF14374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR23725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="363" mass="39288" checksum="135A8BA07C8510D2" modified="2009-07-28" version="1">MSRPQVSVHGLTGETSSSSVALPAVFSAPIRPDIVHSVFTSVNKNKRQAYAVSDKAGHQTSAESWGTGRAVARIPRVGGGGTHRSGQAAFGNMCRGGRMFAPTKTWRKWNVKVNHNEKRYATASAIAASAVASLVLARGHRVEKIPEIPLVVSNDLESVKKTKEAVAALKAVGAHSDVVKVLKSKKMRAGKGKYRNRRFTQRRGPLVVYAEDNGIVKAFRNLPGVETANVASLSLLQLAPGAHMGRFVIWTESAFAKLDQVWGSETVASAVKSGYTLPSNIISNTDVTRIINSSEIQSVVRPAGQPSQKRTHVLKKNPLKNKQVLLRLNPYAKVFAAEKLGSKKVEKKTSTPSKIFSETLKHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIY2</accession>
    <name>C5DIY2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphoribosylaminoimidazole-succinocarboxamide synthase</fullName>
            <ecNumber evidence="3">6.3.2.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">SAICAR synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0E16082g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SAICAR synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.6" evidence="3"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554180.1">
        <property type="nucleotide sequence ID" value="XM_002554134.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIY2"/>
    <dbReference type="FunCoup" id="C5DIY2">
        <property type="interactions" value="821"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIY2"/>
    <dbReference type="GeneID" id="8292351"/>
    <dbReference type="KEGG" id="lth:KLTH0E16082g"/>
    <dbReference type="eggNOG" id="KOG2835">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045637_0_2_1"/>
    <dbReference type="InParanoid" id="C5DIY2"/>
    <dbReference type="OMA" id="CEPFKVE"/>
    <dbReference type="OrthoDB" id="9991235at2759"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00131"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004639">
        <property type="term" value="F:phosphoribosylaminoimidazolesuccinocarboxamide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01414">
        <property type="entry name" value="SAICAR_synt_Sc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000392">
        <property type="entry name" value="Phosphoribosylaminoimidazole-succinocarboxamide synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000015">
        <property type="entry name" value="Phosphoribosylaminoimidazole-succinocarboxamide synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00137">
        <property type="entry name" value="SAICAR_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028923">
        <property type="entry name" value="SAICAR_synt/ADE2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001636">
        <property type="entry name" value="SAICAR_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018236">
        <property type="entry name" value="SAICAR_synthetase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010568">
        <property type="entry name" value="PRK13961.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00081">
        <property type="entry name" value="purC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43700">
        <property type="entry name" value="PHOSPHORIBOSYLAMINOIMIDAZOLE-SUCCINOCARBOXAMIDE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43700:SF1">
        <property type="entry name" value="PHOSPHORIBOSYLAMINOIMIDAZOLE-SUCCINOCARBOXAMIDE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01259">
        <property type="entry name" value="SAICAR_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01057">
        <property type="entry name" value="SAICAR_SYNTHETASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01058">
        <property type="entry name" value="SAICAR_SYNTHETASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAICAR synthetase/ADE2 N-terminal" evidence="11">
        <location>
            <begin position="15"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="145"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR23743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="304" mass="34303" checksum="0DCB6F806958B4F1" modified="2009-07-28" version="1">MALRETNLDGILPLLAKGKVRDIYEVDSKTLLFVATDRISAYDVIMKNTIPDKGVLLTKLSEFWFSYLSDDVRNHLVEIPENKTIFDLLPAQLSEPKYKSQLEGRCMLIQKFKLIPLEVIVRGYITGSAWKEYKKTGTVHGLEAPSGLQESEEFPTPIFTPSTKAEQGEHDENISPAQAAALVGQDLCDRVASLAIKLYSKCKDYARTKGIIIADTKFEFGIDESTNEIILVDEVLTPDSSRFWNGQNYKIGQPQDSYDKQFLRNWLTANKLNGVDGVSMPEDIVRNTRSKYVEAYETLTGENF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJU0</accession>
    <name>C5DJU0_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">1,3-beta-glucanosyltransferase</fullName>
            <ecNumber evidence="12">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0F19096g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Splits internally a 1,3-beta-glucan molecule and transfers the newly generated reducing end (the donor) to the non-reducing end of another 1,3-beta-glucan molecule (the acceptor) forming a 1,3-beta linkage, resulting in the elongation of 1,3-beta-glucan chains in the cell wall.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell envelope</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Cell membrane</location>
            <topology evidence="12">Lipid-anchor</topology>
            <topology evidence="12">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the glycosyl hydrolase 72 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="12"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555016.1">
        <property type="nucleotide sequence ID" value="XM_002554970.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJU0"/>
    <dbReference type="FunCoup" id="C5DJU0">
        <property type="interactions" value="45"/>
    </dbReference>
    <dbReference type="GeneID" id="8293252"/>
    <dbReference type="KEGG" id="lth:KLTH0F19096g"/>
    <dbReference type="eggNOG" id="ENOG502QRZZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021855_1_2_1"/>
    <dbReference type="InParanoid" id="C5DJU0"/>
    <dbReference type="OMA" id="WTQEAND"/>
    <dbReference type="OrthoDB" id="421038at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042124">
        <property type="term" value="F:1,3-beta-glucanosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071970">
        <property type="term" value="P:fungal-type cell wall (1-&gt;3)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000032">
        <property type="entry name" value="1,3-beta-glucanosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004886">
        <property type="entry name" value="Glucanosyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31468">
        <property type="entry name" value="1,3-BETA-GLUCANOSYLTRANSFERASE GAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31468:SF14">
        <property type="entry name" value="1,3-BETA-GLUCANOSYLTRANSFERASE GAS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03198">
        <property type="entry name" value="Glyco_hydro_72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 12" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="10 12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 12 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0732">Signal</keyword>
    <keyword evidence="5 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005125169" description="1,3-beta-glucanosyltransferase" evidence="12">
        <location>
            <begin position="24"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="465"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="431"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR24579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="489" mass="55275" checksum="FAF19071EEFE1570" modified="2009-07-28" version="1">MISLLTTVLLSLLSGLFAGLAEGLVNPIEIHEQHFFDSITGELFFIKGVDYQPGGSSAITSDQDPLSDPKVCARDIALFQQLGINTIRVYSISPDLNHDSCMSMLAAAGIYLILDVNSPMENQHLNRYEPWTTYNHEYLQHVFEIIEEFGHYNNTLGFFAGNEIINDEVSAERSPVYVKAVVNDMKQYIKANAPRKIPVGYSAADDLRYRIPLSKYLECEDEDMPDSSIDFYGVNSYQWCGKQTFQTSGYDQLVKAYLEYTKPVFFSEFGCNQVSPRSFEEVESLHSKDMVNTFSGGLVYEFTQEANKYGLVEIDTNGDAHLLSDFKALQKRYTAIESKEFQTVRENAISLMAMAPQPVCESSYENLDITPTVPKNLAQELIRKRVENKRGKYIKLSQSDMVSTFDVYDENGDQFESELAIKTINQIDSDDVDDKHEFEPEDPSPPKSPSKERERNSAPKPTSNVYLIAAWFASYLFLVACSQATVFNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJV9</accession>
    <name>C5DJV9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">tRNA wybutosine-synthesizing protein 4</fullName>
            <ecNumber evidence="5">2.1.1.290</ecNumber>
            <ecNumber evidence="4">2.3.1.231</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">tRNA(Phe) (7-(3-amino-3-(methoxycarbonyl)propyl)wyosine(37)-N)-methoxycarbonyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">tRNA(Phe) (7-(3-amino-3-carboxypropyl)wyosine(37)-O)-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">KLTH0F19514g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>7-[(3S)-(3-amino-3-methoxycarbonyl)propyl]wyosine(37) in tRNA(Phe) + S-adenosyl-L-methionine + CO2 = wybutosine(37) in tRNA(Phe) + S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:37119"/>
            <dbReference type="Rhea" id="RHEA-COMP:11844"/>
            <dbReference type="Rhea" id="RHEA-COMP:11847"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73544"/>
            <dbReference type="ChEBI" id="CHEBI:74275"/>
            <dbReference type="EC" id="2.3.1.231"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in tRNA(Phe) + S-adenosyl-L-methionine = 7-[(3S)-(3-amino-3-methoxycarbonyl)propyl]wyosine(37) in tRNA(Phe) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:36903"/>
            <dbReference type="Rhea" id="RHEA-COMP:10379"/>
            <dbReference type="Rhea" id="RHEA-COMP:11844"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73543"/>
            <dbReference type="ChEBI" id="CHEBI:74275"/>
            <dbReference type="EC" id="2.1.1.290"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">tRNA modification; wybutosine-tRNA(Phe) biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the methyltransferase superfamily. LCMT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.290" evidence="5"/>
    <dbReference type="EC" id="2.3.1.231" evidence="4"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555035.1">
        <property type="nucleotide sequence ID" value="XM_002554989.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJV9"/>
    <dbReference type="FunCoup" id="C5DJV9">
        <property type="interactions" value="121"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJV9"/>
    <dbReference type="GeneID" id="8293271"/>
    <dbReference type="KEGG" id="lth:KLTH0F19514g"/>
    <dbReference type="eggNOG" id="KOG2918">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002761_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJV9"/>
    <dbReference type="OMA" id="FCILEQF"/>
    <dbReference type="OrthoDB" id="47172at2759"/>
    <dbReference type="UniPathway" id="UPA00375"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008175">
        <property type="term" value="F:tRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031591">
        <property type="term" value="P:wybutosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011043">
        <property type="entry name" value="Gal_Oxase/kelch_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007213">
        <property type="entry name" value="Ppm1/Ppm2/Tcmp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46529">
        <property type="entry name" value="TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46529:SF1">
        <property type="entry name" value="TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13418">
        <property type="entry name" value="Beta-prop_TYW4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04072">
        <property type="entry name" value="LCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50965">
        <property type="entry name" value="Galactose oxidase, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0819">tRNA processing</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="670"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAR24598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="699" mass="78315" checksum="BAA9056E956BBB9D" modified="2009-07-28" version="1">MTPLVSEAGNKQNDLTPRQARQAEKLERRKKYADLAIQGTNNSSIASKRSVERLYLHHMDANRNVDENKGCNEYFKYFVNKALRRSPCINRGYWLRIYAIKSKIDSIAASTNKPITVINLGCGFDPLPFQLLDSTNKENAKYGGRLSFVDLDYPDLLLEKKKIIEKTPELQQIVGKLIAHPRLQNVLQGRQYLASPCDLNKAGNFVKLMEGLELNDPNKIKIFVAEVSLAYMSSEKSNAIILSCSKMPNSHFVVLEQLVPAGPSEPFSRRMLYHFKKNDSPLQSVLDHQTKATQEQRFRSYGFPHTNIGNMYQLWESIDDDIKQKVESVEPFDELEEFLMFCHHYVIGHATNDQNFEFCPKLKQNEELNPSELLIKDPPMDFLPLDGSDFLQRKFGSAAILPNNEIIYTQGCFSSRLKDTLLIDPVLRSIRQIDPELIGEAPPERMCHTLTSLSENLCVLVGGRGGPKKPYSDLWLLHRKSPDSWQWTAGPSLPETRFRHGACSISDTQVLIFGGVSEGQPFLIYDAGENNLTVPRVEGSIPLVASPGLAYNKKARKGAITGGADKDGSITGAISVFSYDETTKTIKIEKQLTHPAFKRYGCKTIFIDDDRLLIVGGFSPDVLFDQHSTVVEACLTSGKVNLVRIPTAVWEEGIPLMAGFELQMSSQRDVYIFGGGAVCYGFGAVWNGVMRIRGADLSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DK05</accession>
    <name>C5DK05_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="18">KLTH0F00792p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">KLTH0F00792g</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ADP + ATP + H(+) = P(1),P(4)-bis(5'-adenosyl) tetraphosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:16577"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58141"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.7.53"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>sulfate + ADP + H(+) = adenosine 5'-phosphosulfate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:16529"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58243"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.7.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the ATP adenylyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554243.1">
        <property type="nucleotide sequence ID" value="XM_002554197.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DK05"/>
    <dbReference type="FunCoup" id="C5DK05">
        <property type="interactions" value="238"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DK05"/>
    <dbReference type="GeneID" id="8292434"/>
    <dbReference type="KEGG" id="lth:KLTH0F00792g"/>
    <dbReference type="eggNOG" id="ENOG502QRAQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049915_1_0_1"/>
    <dbReference type="InParanoid" id="C5DK05"/>
    <dbReference type="OMA" id="DPFENPP"/>
    <dbReference type="OrthoDB" id="10267950at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003877">
        <property type="term" value="F:ATP:ADP adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008796">
        <property type="term" value="F:bis(5'-nucleosyl)-tetraphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004780">
        <property type="term" value="F:sulfate adenylyltransferase (ADP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009164">
        <property type="term" value="P:nucleoside catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.428.70:FF:000001">
        <property type="entry name" value="APA1p AP4A phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009163">
        <property type="entry name" value="Ap4A_phos1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043171">
        <property type="entry name" value="Ap4A_phos1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045759">
        <property type="entry name" value="Ap4A_phos1/2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019200">
        <property type="entry name" value="ATP_adenylylTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38420">
        <property type="entry name" value="AP-4-A PHOSPHORYLASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38420:SF1">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_5G14690)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19327">
        <property type="entry name" value="Ap4A_phos_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09830">
        <property type="entry name" value="ATP_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000846">
        <property type="entry name" value="ATP_adenylyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ap4A phosphorylase 1/2 N-terminal" evidence="17">
        <location>
            <begin position="3"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="ATP adenylyltransferase C-terminal" evidence="16">
        <location>
            <begin position="192"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="14">
        <location>
            <position position="155"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="90"/>
            <end position="91"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="148"/>
            <end position="151"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="269"/>
            <end position="271"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="276"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00050267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00050541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00061129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000846-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000846-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF09830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF19327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAR23806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="317" mass="36229" checksum="F9A3AFE2DE1D3F6B" modified="2009-07-28" version="1">MAQDLATKVRETYANAAKNGYVHFFQSDSKKLKDPETGVQYMVTHAPSLLKKPERGDKEAEKRNPFAEPEPELTVCEDLFGEGEYKLLLNKFPVVPEHLLLVTKEFKPQTSTLSPKDLITAYKLLQELDDEDEMVRNVVFYNCGASSGSSQDHKHLQMFKLPEKFTLFQDRLCNGKEHYVPNVRTEPLQDSKLSFAHFAVPLPDEKEVDEELLAMTFVSLLQRTLTFFQDWTNEKPELETGYNVLLTKEWLCVVPRSASKSKELNIGLNATGYAGLVLVKHEDVWEKLQENPHLIDKLLLECGFPNTAGQSSNEYHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DK16</accession>
    <name>C5DK16_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="2 6">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0F01034g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the FKBP-type PPIase family. FKBP2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="2 6"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554254.1">
        <property type="nucleotide sequence ID" value="XM_002554208.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DK16"/>
    <dbReference type="FunCoup" id="C5DK16">
        <property type="interactions" value="597"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DK16"/>
    <dbReference type="GeneID" id="8292445"/>
    <dbReference type="KEGG" id="lth:KLTH0F01034g"/>
    <dbReference type="eggNOG" id="KOG0549">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013615_8_2_1"/>
    <dbReference type="InParanoid" id="C5DK16"/>
    <dbReference type="OMA" id="VHMHYTG"/>
    <dbReference type="OrthoDB" id="1902587at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.40:FF:000006">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044609">
        <property type="entry name" value="FKBP2/11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45779">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45779:SF7">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0697">Rotamase</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950509" description="peptidylprolyl isomerase" evidence="7">
        <location>
            <begin position="22"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase FKBP-type" evidence="8">
        <location>
            <begin position="47"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR23817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="141" mass="15289" checksum="7920BB86BBA621EF" modified="2009-07-28" version="1">MLFKNLLCFLSLSSLSSISMAGSLTELEIKTIEEVPASECTIKASKGDLVSVHYSGSLLGSEEVFDSSYGRGVPIKFELGAGQVIKGWDQGILDMCIGERRELRIPSQLGYGSRGAGGVIPPNADLFFETTLVDIRRRDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DK29</accession>
    <name>C5DK29_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptide-methionine (R)-S-oxide reductase</fullName>
            <ecNumber evidence="5">1.8.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0F01320g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-methionyl-[protein] + [thioredoxin]-disulfide + H2O = L-methionyl-(R)-S-oxide-[protein] + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:24164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="Rhea" id="RHEA-COMP:12313"/>
            <dbReference type="Rhea" id="RHEA-COMP:12314"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16044"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:45764"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.8.4.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="5">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the MsrB Met sulfoxide reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.4.12" evidence="5"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554267.1">
        <property type="nucleotide sequence ID" value="XM_002554221.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DK29"/>
    <dbReference type="FunCoup" id="C5DK29">
        <property type="interactions" value="316"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DK29"/>
    <dbReference type="GeneID" id="8292458"/>
    <dbReference type="KEGG" id="lth:KLTH0F01320g"/>
    <dbReference type="eggNOG" id="KOG0856">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031040_8_1_1"/>
    <dbReference type="InParanoid" id="C5DK29"/>
    <dbReference type="OMA" id="YDETTDW"/>
    <dbReference type="OrthoDB" id="44061at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033743">
        <property type="term" value="F:peptide-methionine (R)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030091">
        <property type="term" value="P:protein repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.20">
        <property type="entry name" value="Peptide methionine sulfoxide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028427">
        <property type="entry name" value="Met_Sox_Rdtase_MsrB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002579">
        <property type="entry name" value="Met_Sox_Rdtase_MsrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00357">
        <property type="entry name" value="peptide-methionine (R)-S-oxide reductase MsrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46081">
        <property type="entry name" value="PEPTIDE METHIONINE SULFOXIDE REDUCTASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46081:SF8">
        <property type="entry name" value="PEPTIDE METHIONINE SULFOXIDE REDUCTASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01641">
        <property type="entry name" value="SelR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="Mss4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51790">
        <property type="entry name" value="MSRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MsrB" evidence="6">
        <location>
            <begin position="38"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU365044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR23830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="166" mass="18920" checksum="352147FFAA8267CE" modified="2009-07-28" version="1">MIRSTARIFRGFAGTRSIVVSRLIYKRSFSISFVTRMSENWNPNLTTEQLLVLRDKHTERPHTGAYLNNKDTGVYNCANCEAPLYKSETKFDSHCGWPAFYEEVKKGAITYHTDYTMGMKRTEICCANCGGHLGHVFEGEGWKERLGLPVDARHCVNSASLNFKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DK38</accession>
    <name>C5DK38_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0F01518p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0F01518g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554276.1">
        <property type="nucleotide sequence ID" value="XM_002554230.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DK38"/>
    <dbReference type="GeneID" id="8292467"/>
    <dbReference type="KEGG" id="lth:KLTH0F01518g"/>
    <dbReference type="eggNOG" id="KOG3017">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_420953_0_0_1"/>
    <dbReference type="InParanoid" id="C5DK38"/>
    <dbReference type="OMA" id="ASTXSTS"/>
    <dbReference type="OrthoDB" id="337038at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018244">
        <property type="entry name" value="Allrgn_V5/Tpx1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001283">
        <property type="entry name" value="CRISP-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002413">
        <property type="entry name" value="V5_allergen-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10334">
        <property type="entry name" value="CYSTEINE-RICH SECRETORY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00838">
        <property type="entry name" value="V5ALLERGEN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00837">
        <property type="entry name" value="V5TPXLIKE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01009">
        <property type="entry name" value="CRISP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950407" evidence="2">
        <location>
            <begin position="19"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="domain" description="SCP" evidence="3">
        <location>
            <begin position="485"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="370"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR23839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="651" mass="61063" checksum="213C80811F2135E3" modified="2009-07-28" version="1">MKYSSIAGLCMAVAAVSAQTTTTDDFTATAIDLTLTKPGATGSSGVTSAPSSSATGSSAAPSGSSVAPSGSSSVPSGSSAAPSGSSAAPSGSSGVPSGSSAAPSGSSAAPSGSSAAPSGSSVAPSGSSSVPSGSSGVPSGSSAAPSGSSAAPSGSSAAPSGSSAAPSGSSSVPSGSSAAPSGSSAAPSGSSAAPSGSSSVPSGSSAAPSGSSAAPSGSSGVPSGSSAAPSGSSAAPSGSSAAPSGTSSVAPSSSAISSGSSSVPSGSIITSGSSSAPVSSSVPSSSFSLSSSAPNSVWYSNTSSTYPSTTGGLTSLPITSDSSSSPSSSESSTTDSSSSDAISTVASSSDVSSSLASSSAVSSVVVSSSDVSSSAPSSSVASSSAPSSSVASSSAPSSSVASSSDTSSILASSSAASSVAVSSSAASSSAPSSSAATSSSATSSSSLLAASASNAAMTTITGSSVTSSSVASSTTASATTTLDAATASAILSEHNAKRSLHSNTPDLTWDDSLSAWAYAYAESLSGTSYDPCSGNLVHSSTRNNQGENIGFGTVSDPNTLVDYWYDEIQYYDYDNVTGITHDGQEVGHFTQLVWASTTKVGCAVIQCDTMAKYGQNSQYLLCEYTPAGNIYNGTPGQDEFSFFKENVQRLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>C5DK67</accession>
    <name>C5DK67_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0F02178p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0F02178g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554305.1">
        <property type="nucleotide sequence ID" value="XM_002554259.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DK67"/>
    <dbReference type="FunCoup" id="C5DK67">
        <property type="interactions" value="48"/>
    </dbReference>
    <dbReference type="GeneID" id="8292497"/>
    <dbReference type="KEGG" id="lth:KLTH0F02178g"/>
    <dbReference type="eggNOG" id="ENOG502S7G5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2005713_0_0_1"/>
    <dbReference type="InParanoid" id="C5DK67"/>
    <dbReference type="OMA" id="LIHEHAY"/>
    <dbReference type="OrthoDB" id="4067115at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="119" mass="13416" checksum="365E40AED5BC9E8D" modified="2009-07-28" version="1">MIPEPVDPGLLREHAFYDSGDLGVVLSKDTYTEFGGFKSIFKYGLGYFNYASGLDREVHERSLAYMLKFHILQHFGLYCCITMVALVVSFIALSQVQGNAMGRNKKAEKDGDDVYYVKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKC6</accession>
    <name>C5DKC6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0F03542p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0F03542g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554364.1">
        <property type="nucleotide sequence ID" value="XM_002554318.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKC6"/>
    <dbReference type="FunCoup" id="C5DKC6">
        <property type="interactions" value="66"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKC6"/>
    <dbReference type="GeneID" id="8292557"/>
    <dbReference type="KEGG" id="lth:KLTH0F03542g"/>
    <dbReference type="eggNOG" id="KOG4406">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027718_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKC6"/>
    <dbReference type="OMA" id="KKISWVY"/>
    <dbReference type="OrthoDB" id="410651at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00159">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45808">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 68F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45808:SF2">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 68F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13716">
        <property type="entry name" value="CRAL_TRIO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="2">
        <location>
            <begin position="191"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="368"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="368"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="439"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR23927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="589" mass="67117" checksum="3FE8649A9F9DCE80" modified="2009-07-28" version="1">MNSTVEVRLDNIFFQSYAKDPASGSAIYVFDSTYLPSADDVESREVYDLLIDEVMDRLVTKLPELPFSLVVFSSGFTQNNVSWIYGVKMFAKIPKSTRARLQKMLIVHESFFVRTVYQVLTNALNINPFKNKNNADAVIHHVANLTELAEYVDITRLRISLNVYLYDYQFNETISVPESYVAEGSSLANRQYRQLIFDKIFKRLQIDAPKHELVFQRPGSYQKVNVLLGVIARNNYVDLSQWDIYSLATVFLHFIKGKTKPVLPIDLIPLPISDSFEYTYNTFVSMMLYNGYYELTLYIFELFLTLVGNSEVTLHDYRTLSKCLTATLCKEKVSLKTSDRLAIGYRYTRNVFMKFHDLRHEIEQKGALKAVEEPRTPRFTREAPKIPPPRKKSPTKACRSTNSSKERIPPTKPPALPKRKGIPSSSTTSLPAADLRAEMSPSPQPMMSRSEVSLDITEPRSPKLGATPNLRLGSNSSINVSMTSDPNVSPQLQPQLIPDEAVTFKSKPEDDLFKLDQNMSSLLLDNNQKIQSLDKELKKKKQASNLGSERFSNSAYSDIKAENKVSRLAALYEERLVGIRVMEDMRRNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKP8</accession>
    <name>C5DKP8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0F06490p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0F06490g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the actin family. ARP2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554486.1">
        <property type="nucleotide sequence ID" value="XM_002554440.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKP8"/>
    <dbReference type="FunCoup" id="C5DKP8">
        <property type="interactions" value="1152"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKP8"/>
    <dbReference type="GeneID" id="8292687"/>
    <dbReference type="KEGG" id="lth:KLTH0F06490g"/>
    <dbReference type="eggNOG" id="KOG0677">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027965_0_2_1"/>
    <dbReference type="InParanoid" id="C5DKP8"/>
    <dbReference type="OMA" id="WEDMQHL"/>
    <dbReference type="OrthoDB" id="5132116at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10220">
        <property type="entry name" value="ASKHA_NBD_Arp2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000050">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000005">
        <property type="entry name" value="Actin-related protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020902">
        <property type="entry name" value="Actin/actin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00190">
        <property type="entry name" value="ACTIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01132">
        <property type="entry name" value="ACTINS_ACT_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR24049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="391" mass="44228" checksum="5D227FBAEABF6D66" modified="2009-07-28" version="1">MDLRTPIVLDQGTGFVKIGRAGENFPDYTFPSIVGRPILRAEERANVVTPLKDIMIGDEASAVRSYLQISYPMENGVIKNWSDMELLWDYAFYDQMKLPSTSGGKVMLTEPPMNPLRNREQMCEVMFEKYDFGGVYVAIQAVLALYAQGLSSGVVVDSGDGVTHIVPVYESVVLNHLTRRLDVAGRDVTRHLIDLLSRRGYAFNRSADFETVRQIKEKLSYVSYDLDLDTKLARETTTLVENYELPDGRVIKVGSERFEAPECLFQPNLVDVEQPGVGEMLFNTIQAADVDVRSALYRAIVLSGGSSMYPGLPSRLEKELKQLWFTRVLRNDPSRLEKFKVRIEDPPRRKHMVFIGGAVLANIMADKDHMWLSKQEWQEMGPSAMSKFGPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKR0</accession>
    <name>C5DKR0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">KLTH0F06754p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0F06754g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554498.1">
        <property type="nucleotide sequence ID" value="XM_002554452.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKR0"/>
    <dbReference type="GeneID" id="8292699"/>
    <dbReference type="KEGG" id="lth:KLTH0F06754g"/>
    <dbReference type="eggNOG" id="KOG1339">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_364105_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKR0"/>
    <dbReference type="OMA" id="FYLANIE"/>
    <dbReference type="OrthoDB" id="771136at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05474">
        <property type="entry name" value="SAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033876">
        <property type="entry name" value="SAP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF65">
        <property type="entry name" value="ASPARTIC-TYPE ENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 7" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950519" evidence="9">
        <location>
            <begin position="30"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="10">
        <location>
            <begin position="82"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="508"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="510"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="537"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="100"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR24061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="767" mass="82464" checksum="8B3E962FAAB79756" modified="2009-07-28" version="1">MSFSVKSTMSVPCAMLALFLALFVPCVIASSGMGYVAIDFAKAKGISYQDARVGARPAKLQKRALSQGETLNSELVNNNVFYSVGLDIGTPPQQVTVLFDTGSSDLWVSSNDNPYCSNGDSTESYANYRADLKSSSALTAAPTTTISGITIAPTIDCAQYGTFNADRSTSLFANYSKPFFVSYNDDSYALGYWARDKLVVDGDDISSLEFAVAEISNSTVGVLGVGLQGLESTVHYRSPNISSIYPNFPMVLKENGAIDKIAYSLYLNSLDAPSGSVLFGGVDHSKYTGGLYTLPLVNTYKDYGVKTPVQFDVTVQGIGIESEKACRQETFTTTKIAALMDSGTTLMYTAPEIVNKIALYVNASFSPGYGLFMFRCPSDGDDTQFIFDFGGFQIKSPLSNYILRTDDENICGLGLISSNQGTIFGDEFLASAYVVYDLENLELSLAQANWNPGSPEIEKIVSEIPRAQRASQYWNTWVSDEENMAQVTRDIFTKTLACSPTNVNSTMPATNSSSFKTLNNSTTSTLTSHSDSSHTLGQNSFRTSVKPHVSSTRNGSIISSTNFATKAPSRTEQATYLFSSTSSVKASHLNHKSHKLAEASTKTPSCKTDKQTIISKARCESTIGPVTRTACRSCSRASKHLTRPSIYTTVTATGTRLITEVATCHLCETEASRAENGKSVREITAECSSKNLKNANAKNKEANTEVWLTNSTQIPIVTVSGPLQSISVSSSTSFEVQLVAGSPNLATPKLSLGAFLFAILVGGALSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKV9</accession>
    <name>C5DKV9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cell division control protein 42</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0F07920g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rho family. CDC42 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554547.1">
        <property type="nucleotide sequence ID" value="XM_002554501.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKV9"/>
    <dbReference type="FunCoup" id="C5DKV9">
        <property type="interactions" value="1015"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKV9"/>
    <dbReference type="GeneID" id="8292752"/>
    <dbReference type="KEGG" id="lth:KLTH0F07920g"/>
    <dbReference type="eggNOG" id="KOG0393">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041217_21_3_1"/>
    <dbReference type="InParanoid" id="C5DKV9"/>
    <dbReference type="OMA" id="GDEPYTF"/>
    <dbReference type="OrthoDB" id="8830751at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051286">
        <property type="term" value="C:cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030427">
        <property type="term" value="C:site of polarized growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030010">
        <property type="term" value="P:establishment of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01874">
        <property type="entry name" value="Cdc42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000236">
        <property type="entry name" value="Cell division control protein 42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037874">
        <property type="entry name" value="Cdc42"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0636">Prenylation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00070025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR24110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="191" mass="21421" checksum="AF418FD3C7B63B38" modified="2009-07-28" version="1">MQTLKCVVVGDGAVGKTCLLISYTTNQFPADYVPTVFDNYAVTVMIGDEPYTLGLFDTAGQEDYDRLRPLSYPSTDVFLVCFSVISPPSFENVKEKWFPEVHHHCPGVPCLIVGTQIDLRDDKVIIEKLQRQRLRPISAEQGERLARELRAIKYVECSALTQRGLKNVFDEAIVAALEPPVIKKSKKCTIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKW4</accession>
    <name>C5DKW4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Kynureninase</fullName>
            <ecNumber evidence="4 5">3.7.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Biosynthesis of nicotinic acid protein 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">L-kynurenine hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">BNA5</name>
        <name evidence="6" type="ordered locus">KLTH0F08052g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 5">Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3-hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3-hydroxyanthranilic acid (3-OHAA), respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>3-hydroxy-L-kynurenine + H2O = 3-hydroxyanthranilate + L-alanine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25143"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:36559"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:58125"/>
            <dbReference type="EC" id="3.7.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 5">
            <text>L-kynurenine + H2O = anthranilate + L-alanine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16813"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <dbReference type="ChEBI" id="CHEBI:57959"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="EC" id="3.7.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4 5">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 5">Amino-acid degradation; L-kynurenine degradation; L-alanine and anthranilate from L-kynurenine: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 5">Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from L-kynurenine: step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the kynureninase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.7.1.3" evidence="4 5"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554552.1">
        <property type="nucleotide sequence ID" value="XM_002554506.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKW4"/>
    <dbReference type="FunCoup" id="C5DKW4">
        <property type="interactions" value="252"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKW4"/>
    <dbReference type="GeneID" id="8292758"/>
    <dbReference type="KEGG" id="lth:KLTH0F08052g"/>
    <dbReference type="eggNOG" id="KOG3846">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003433_4_0_1"/>
    <dbReference type="InParanoid" id="C5DKW4"/>
    <dbReference type="OMA" id="LPGWNSH"/>
    <dbReference type="OrthoDB" id="5978656at2759"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00329"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00334">
        <property type="reaction ID" value="UER00455"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030429">
        <property type="term" value="F:kynureninase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034354">
        <property type="term" value="P:'de novo' NAD+ biosynthetic process from L-tryptophan"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043420">
        <property type="term" value="P:anthranilate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097053">
        <property type="term" value="P:L-kynurenine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019441">
        <property type="term" value="P:L-tryptophan catabolic process to kynurenine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019805">
        <property type="term" value="P:quinolinate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000031">
        <property type="entry name" value="Kynureninase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01970">
        <property type="entry name" value="Kynureninase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010111">
        <property type="entry name" value="Kynureninase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01814">
        <property type="entry name" value="kynureninase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14084">
        <property type="entry name" value="KYNURENINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14084:SF0">
        <property type="entry name" value="KYNURENINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22580">
        <property type="entry name" value="KYNU_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038800">
        <property type="entry name" value="KYNU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 4 5" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="3 4 5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="4">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="145"/>
            <end position="148"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="293"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="321"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="4">
        <location>
            <position position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR038800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR24115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="462" mass="51792" checksum="EB4D8BF1454540DD" modified="2009-07-28" version="1">MEKAVELDNQYPATHRDAMCIPTYESMGLRAPSANFSKFSPVTYLCGNSLGCMPRATREAVNSELDAWADRAVESHFRHRGEPQGLTSWVDIDLPLSNLLPSVVGGLESEVAVMGSLTANLNALLVAFYRPNSQKFKIICEKGAFPSDFYAFLNQCAIHGVDPEEALVRLEPREGETYLRTDDIIKAISENSEQLALVCLPGIQYYTGQLFDMKKITLYAHQFEDVVVGWDLAHAVGNVPLQLHDWGVDFACWCSYKYLNAGPGGIAGIFVHEKHAQVASHTQEGYMPRLAGWWGNNRERRFQMKEVFEPISGALGFRQSNPSVIDVVALRSSLEVIKDFGGIEALRERSLLLTGYLLKLLQNSPFYHESLTATPQSKPGFTIITPLDEDERGAQLSLLFSPHHDDPKLDTMEQVFKYLNERGVIVDERRPDVIRIAPAPLYNTFQDVFRTVDLLNKALQHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKX5</accession>
    <name>C5DKX5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0F08294p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0F08294g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554563.1">
        <property type="nucleotide sequence ID" value="XM_002554517.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKX5"/>
    <dbReference type="FunCoup" id="C5DKX5">
        <property type="interactions" value="79"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKX5"/>
    <dbReference type="GeneID" id="8292769"/>
    <dbReference type="KEGG" id="lth:KLTH0F08294g"/>
    <dbReference type="eggNOG" id="KOG1337">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041939_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKX5"/>
    <dbReference type="OMA" id="YWGDYTI"/>
    <dbReference type="OrthoDB" id="341421at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19177">
        <property type="entry name" value="SET_SETD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1410.10">
        <property type="entry name" value="set domain protein methyltransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
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    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
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    <dbReference type="InterPro" id="IPR016852">
        <property type="entry name" value="SET_MeTrfase"/>
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    <dbReference type="InterPro" id="IPR050600">
        <property type="entry name" value="SETD3_SETD6_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044429">
        <property type="entry name" value="SETD4_SET"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271:SF47">
        <property type="entry name" value="ACTIN-HISTIDINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271">
        <property type="entry name" value="UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF027158">
        <property type="entry name" value="Lys_MTase_YDR198C_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SET" evidence="4">
        <location>
            <begin position="31"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR24126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="464" mass="53140" checksum="D9C72751950CAA14" modified="2009-07-28" version="1">MAATSQSLPWDERLDYLLKWLEESEGFAVNEHVRLQDTKRSGRGLYLKSGALKKRDCIVSIPGDKQLNFYTVIYHISLFNCELAIDGVSCPSSDRANNKIYEADDPRTEAYRHLTNNVVLGLSSFQLLSLFILFEWKILPQESNFTSFWKPFFDVFPSFEDLRSIPATWVCDPESGNLELIEMLPAASKKHAERMVSQVKHDWETIFPFIQKWLESREGASQINKQKLFSEFVHVYFVINSRCLYIEIPLKTDVADNFTMVPFVDFLNHNSDVDAYCKPRIERLKKSPCGLGNFSIVAGDHEYVNLGEEILLNYGAHSNDFLLNEYGFVLGENMWNYIDVSSEAMELISQVHVKEFLIENNYWGDYTISSSEVSFRLLVAFAAVVCSDLRKVEKFMLGYITESSFGSALSSMLREFIASLTKNVETKVRSLKATSKPDPLCSQNVLHVYEGYLQILRQHLGDNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>C5DL67</accession>
    <name>C5DL67_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="19">KLTH0F10406p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="ordered locus">KLTH0F10406g</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554655.1">
        <property type="nucleotide sequence ID" value="XM_002554609.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DL67"/>
    <dbReference type="FunCoup" id="C5DL67">
        <property type="interactions" value="337"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DL67"/>
    <dbReference type="GeneID" id="8292865"/>
    <dbReference type="KEGG" id="lth:KLTH0F10406g"/>
    <dbReference type="eggNOG" id="KOG1002">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000315_2_1_1"/>
    <dbReference type="InParanoid" id="C5DL67"/>
    <dbReference type="OMA" id="DHIMLRR"/>
    <dbReference type="OrthoDB" id="448448at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000109">
        <property type="term" value="C:nucleotide-excision repair complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008094">
        <property type="term" value="F:ATP-dependent activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18008">
        <property type="entry name" value="DEXDc_SHPRH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16567">
        <property type="entry name" value="RING-HC_RAD16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000753">
        <property type="entry name" value="DNA repair protein RAD16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000080">
        <property type="entry name" value="DNA repair protein RAD16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001864">
        <property type="entry name" value="DNA repair protein RAD16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050628">
        <property type="entry name" value="SNF2_RAD54_helicase_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626:SF12">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0347">Helicase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="17">
        <location>
            <begin position="172"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="512"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="18">
        <location>
            <begin position="598"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="20"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="55"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="69"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="94"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAR24218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="765" mass="88113" checksum="41FD45900FA93585" modified="2009-07-28" version="1">MLEEGGFIRKTRTRRKRKVDYKEDSDGELVRNDTSADDNYSDLGKEETPVIIESSDEKPNLDKLIEIKSEEEDEDEDDLPLAKRRKADASPAKGAKKGAKGKKKSTAKPKKPKTTPYERNTARLFENHPYLKTVFSDLSNAPAYKAHRAPQPEGLTIKLLPFQLEGLYWLIAQEQSIYNGGVLADEMGMGKTIQTIALLMNDVSKKPSLVVAPTVALMQWKNEIEQHTGGKLKTYIYHGANRTSNVGEFKDVDVLLTTYSVLESVFRKQTYGFRRKNGVYKERSVLHNMNFYRVILDEAHNIKDRQSNTAKAVNSLITEKKWCLTGTPLQNRIGEMYSLIRFLNIDPFSKYFCTKCNCESREWKFTDRMHCDGCGHVVMQHTNFFNHFALKNIQKHGIEGPGLESFQNIQTLLKNIMLRRTKVERADDLGLPPRIVTVRRDYFNEEEKDLYRSLYTDVKRKFNSYVEEGVVLNNYANIFTLITRMRQLADHPDLVLKRMKNGIGVDDNVIVCQLCDDEAEEPIESKCHHKFCRLCIKEYIESFMENLEKLTCPVCHIALSIDLSQPALEFDDAAQKKQSIVNRLNIQGSWRSSTKIEALVEELYNLRSDRRTIKSIVFSQFTSMLDLVEWRLKRAGFQTVKLQGSMTPTQRDQTIKYFMENTHCEVFLVSLKAGGVALNLCEASQVFIMDPWWNPSVEWQSGDRVHRIGQYRPVKITRFCIEDSIESRIIELQEKKANMIHATINQDDAAINRLTPGDLQFLFNN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-05-31" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLA5</accession>
    <name>SHE3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName>SWI5-dependent HO expression protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">SHE3</name>
        <name type="ordered locus">KLTH0F11396g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">RNA-binding protein that binds specific mRNAs including the ASH1 mRNA, coding for a repressor of the HO endonuclease. Part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud and in the daughter cell. Required for the delivery of cortical endoplasmic reticulum into the emerging bud.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SHE3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554693.1">
        <property type="nucleotide sequence ID" value="XM_002554647.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLA5"/>
    <dbReference type="SMR" id="C5DLA5"/>
    <dbReference type="FunCoup" id="C5DLA5">
        <property type="interactions" value="1503"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLA5"/>
    <dbReference type="GeneID" id="8292906"/>
    <dbReference type="KEGG" id="lth:KLTH0F11396g"/>
    <dbReference type="eggNOG" id="ENOG502QSQX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038734_0_0_1"/>
    <dbReference type="InParanoid" id="C5DLA5"/>
    <dbReference type="OMA" id="HFMANIN"/>
    <dbReference type="OrthoDB" id="6088208at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048309">
        <property type="term" value="P:endoplasmic reticulum inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031398">
        <property type="entry name" value="She3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17078">
        <property type="entry name" value="SHE3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0175">Coiled coil</keyword>
    <keyword id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0509">mRNA transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000408933" description="SWI5-dependent HO expression protein 3">
        <location>
            <begin position="1"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="284"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="101"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="293"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="320"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="347"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="391"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="410"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="423" mass="46671" checksum="C789E649A8119EA6" modified="2009-07-28" version="1">MSNQEVDFQAAHLESPMKMSPSKVSMNHGAFMANMQNGYSPTREGATAGSSSTRVIEALHAQIDSLTKTNLDLTVQSNNLLSRLESSNNTQSKHLESISTLKHENDNLGLMLSRKERRVRDLEQQLSQLKHSYEEAAMDNKTMRHQLQTSGQREGTLENQLQQLQVQYDALVDGQKRYREKYDLEVEELKKSLQDFKRDNEMYLTKNIQTVVSNNTALQTKINQYSGKYKNLESLSQQHMQELSREVEGMASKLDLAKWEKLYEESRNMAIEYSEKTNVPLSPSFLAQHGAKSGSRSPSTSSSSTFVHPSQIRVPKVRHSSSSAKRSSFYGSNVSVPGGTVPGVKQPSASPTTPSSGGLPGVRRSSSVRGSSSSSRNSSGDLASAESAAQSQKGASKNSANNKKKRMSSHSYSKSNGFSFGEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLC2</accession>
    <name>C5DLC2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0F11792p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0F11792g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554710.1">
        <property type="nucleotide sequence ID" value="XM_002554664.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLC2"/>
    <dbReference type="STRING" id="559295.C5DLC2"/>
    <dbReference type="GeneID" id="8292924"/>
    <dbReference type="KEGG" id="lth:KLTH0F11792g"/>
    <dbReference type="eggNOG" id="ENOG502QTT5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078586_1_0_1"/>
    <dbReference type="InParanoid" id="C5DLC2"/>
    <dbReference type="OMA" id="GDGAPWH"/>
    <dbReference type="OrthoDB" id="6423603at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024500">
        <property type="entry name" value="DUF3074"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40370:SF1">
        <property type="entry name" value="DUF3074 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40370">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11274">
        <property type="entry name" value="DUF3074"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF3074" evidence="1">
        <location>
            <begin position="72"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF11274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="254" mass="28817" checksum="0C669F9DF1ADC90F" modified="2009-07-28" version="1">MPFEITTKALRADQLPTRKADIASAAQELIDEVHTSWKKGKCYQYKLQHNPTLDAGPVSVQTYSTTRAGEYWLSRVSEHRLEPAVYERLVKELNGSVRDGDGWTLPDRSARSRREIDYIEVLSRVDVAQTLESGWVLVNLEYELGKPLTTREFNEWVYVLEPSRGAQRERSSVVSVVADAPLHDSVAHTPAVYASVEQLDYDYETQKLIWTMATTSDAGGNVPKWIQNTMIAKTVSKDVGYCFEWLAKNMEAEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLE9</accession>
    <name>C5DLE9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">KLTH0F12518p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0F12518g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554737.1">
        <property type="nucleotide sequence ID" value="XM_002554691.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLE9"/>
    <dbReference type="FunCoup" id="C5DLE9">
        <property type="interactions" value="152"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLE9"/>
    <dbReference type="GeneID" id="8292957"/>
    <dbReference type="KEGG" id="lth:KLTH0F12518g"/>
    <dbReference type="eggNOG" id="KOG4157">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024893_1_1_1"/>
    <dbReference type="InParanoid" id="C5DLE9"/>
    <dbReference type="OMA" id="HINMKRE"/>
    <dbReference type="OrthoDB" id="5985073at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051836">
        <property type="entry name" value="Kremen_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002889">
        <property type="entry name" value="WSC_carb-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24269">
        <property type="entry name" value="KREMEN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24269:SF16">
        <property type="entry name" value="PROTEIN SLG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01822">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00321">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51212">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950523" evidence="9">
        <location>
            <begin position="17"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="250"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="WSC" evidence="10">
        <location>
            <begin position="17"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="113"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="148"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="228"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="307"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="228"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR24300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="350" mass="34918" checksum="363D381B982C4DD5" modified="2009-07-28" version="1">MRFFSLCLVLASVCMADLTSLGCYGSLPSDYSGGDSYTWQSSSHCNSVCASKNAKYFALYNAGSCYCGNSSPTDETDSSSCNTACNGWASEMCGGSDAYTVFKVDSSSAGSVTTGSSIAAPSGAGSSSGSASSSGSASSSAASSSAASSSSASSSASSSTSSSSSSSSSTSSNSPSQASSSGSSSSPQSSTAAPTSSTTSSASVNVVITTQVQTDGGVAFTVTATASATASSAPSETAQAHSKKSKPKNLGAIIGGVVGGVCGAAVIAGIALLVLRRLSKKREQERMEKEYQEAIKPVEYRDTLYRSSLSRKGTNPFDDTRRISNGSIMDEPTLTAPNAPKTLTVANPDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLM1</accession>
    <name>C5DLM1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Histone H2A.Z-specific chaperone CHZ1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Histone H2A.Z-specific chaperone chz1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0G01782g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Forms a chaperone-bound H2A.Z-H2B complex that acts as a source for SWR1 complex-dependent H2A to H2A.Z histone replacement in chromatin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CHZ1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555119.1">
        <property type="nucleotide sequence ID" value="XM_002555073.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLM1"/>
    <dbReference type="FunCoup" id="C5DLM1">
        <property type="interactions" value="47"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLM1"/>
    <dbReference type="GeneID" id="8293377"/>
    <dbReference type="KEGG" id="lth:KLTH0G01782g"/>
    <dbReference type="eggNOG" id="ENOG502SCUM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_126134_1_0_1"/>
    <dbReference type="InParanoid" id="C5DLM1"/>
    <dbReference type="OMA" id="RTHYDDE"/>
    <dbReference type="OrthoDB" id="4174291at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019098">
        <property type="entry name" value="Histone_chaperone_domain_CHZ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09649">
        <property type="entry name" value="CHZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01082">
        <property type="entry name" value="CHZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histone chaperone" evidence="9">
        <location>
            <begin position="68"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="23"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="50"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="67"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="92"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR24682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="146" mass="16645" checksum="93251E30A5A7B764" modified="2009-07-28" version="1">MAETKKHELKDQPDKKRVKRRRNYDDYDKEVAAEDSKAKKENKGTGDNGSESESEDDEKLDMMLNKEDEEEDDLAEIDSSNIIQGGRRTRGKVIDYKKTAEALDKESGKASEEPKEELAQKDVAKKSETAEEDEDAEDADFKEPEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLM4</accession>
    <name>C5DLM4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0G01848p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0G01848g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 5'-AMP-activated protein kinase beta subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555122.1">
        <property type="nucleotide sequence ID" value="XM_002555076.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLM4"/>
    <dbReference type="FunCoup" id="C5DLM4">
        <property type="interactions" value="440"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLM4"/>
    <dbReference type="GeneID" id="8293380"/>
    <dbReference type="KEGG" id="lth:KLTH0G01848g"/>
    <dbReference type="eggNOG" id="KOG1616">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033562_1_0_1"/>
    <dbReference type="InParanoid" id="C5DLM4"/>
    <dbReference type="OMA" id="MEQYYVA"/>
    <dbReference type="OrthoDB" id="531008at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031588">
        <property type="term" value="C:nucleotide-activated protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140767">
        <property type="term" value="F:enzyme-substrate adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001403">
        <property type="term" value="P:invasive growth in response to glucose limitation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043254">
        <property type="term" value="P:regulation of protein-containing complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02859">
        <property type="entry name" value="E_set_AMPKbeta_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000562">
        <property type="entry name" value="Snf1 kinase complex beta-subunit Gal83"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.250.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032640">
        <property type="entry name" value="AMPK1_CBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006828">
        <property type="entry name" value="ASC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037256">
        <property type="entry name" value="ASC_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050827">
        <property type="entry name" value="CRP1_MDG1_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10343">
        <property type="entry name" value="5'-AMP-ACTIVATED PROTEIN KINASE , BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10343:SF84">
        <property type="entry name" value="5'-AMP-ACTIVATED PROTEIN KINASE SUBUNIT BETA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16561">
        <property type="entry name" value="AMPK1_CBM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04739">
        <property type="entry name" value="AMPKBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01010">
        <property type="entry name" value="AMPKBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160219">
        <property type="entry name" value="AMPKBI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Association with the SNF1 complex (ASC)" evidence="6">
        <location>
            <begin position="297"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="215"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="91"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="215"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR24685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="416" mass="46294" checksum="52612831F6D94C2E" modified="2009-07-28" version="1">MGNTHSKRAMSENMKDVSMVDVSSAEDEDKKGSQRAIVQSFSKLDIGDHSSSGAPLMRRRSTLIFDDEDESPRHEENVGDQADLNEFGGPSSESSSSASVFHQRQQQTPPAQESEPPAAEAPRVMVPVEITWQQGGSKVYVTGSFTGWRKMIGLVPVADQPGVFHIKLQLPPGTHRFRFIVDNELRFSDYLATATDQMGNFVNYLEISPQDSQQQLQLQQQQQQQQQQQPQQLQPLQQPQQPQPQEQPQHPANYPPRSRRAEAAAEPLSARSKLALQIEEEPDDMGDGYSRFHGGAEATPAYSYTTDIPAVFTDSSVMEQYYLTLDQQQNNQQSMAWLTPPQLPPHLENVILNNYSNSSDPGGSGSENNSGALPIPNHVVLNHLATSSIKHNTLCVASIVRYKRKYATQILYSPLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>C5DM60</accession>
    <name>C5DM60_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 10">Multifunctional fusion protein</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="10">Delta-1-pyrroline-5-carboxylate dehydrogenase</fullName>
                <shortName evidence="10">P5C dehydrogenase</shortName>
            </recommendedName>
            <alternativeName>
                <fullName evidence="10">L-glutamate gamma-semialdehyde dehydrogenase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="9">L-glutamate gamma-semialdehyde dehydrogenase</fullName>
                <ecNumber evidence="9">1.2.1.88</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0G06226g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 9">
            <text>L-glutamate 5-semialdehyde + NAD(+) + H2O = L-glutamate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:30235"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58066"/>
            <dbReference type="EC" id="1.2.1.88"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.88" evidence="9"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555308.1">
        <property type="nucleotide sequence ID" value="XM_002555262.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DM60"/>
    <dbReference type="FunCoup" id="C5DM60">
        <property type="interactions" value="404"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DM60"/>
    <dbReference type="GeneID" id="8293576"/>
    <dbReference type="KEGG" id="lth:KLTH0G06226g"/>
    <dbReference type="eggNOG" id="KOG2455">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_4_1_1"/>
    <dbReference type="InParanoid" id="C5DM60"/>
    <dbReference type="OMA" id="FAGIHFT"/>
    <dbReference type="OrthoDB" id="5322683at2759"/>
    <dbReference type="UniPathway" id="UPA00261">
        <property type="reaction ID" value="UER00374"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003842">
        <property type="term" value="F:L-glutamate gamma-semialdehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010133">
        <property type="term" value="P:L-proline catabolic process to L-glutamate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000006">
        <property type="entry name" value="1-pyrroline-5-carboxylate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000005">
        <property type="entry name" value="1-pyrroline-5-carboxylate dehydrogenase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005931">
        <property type="entry name" value="P5CDH/ALDH4A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050485">
        <property type="entry name" value="Proline_metab_enzyme"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01236">
        <property type="entry name" value="D1pyr5carbox1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42862">
        <property type="entry name" value="DELTA-1-PYRROLINE-5-CARBOXYLATE DEHYDROGENASE 1, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42862:SF1">
        <property type="entry name" value="DELTA-1-PYRROLINE-5-CARBOXYLATE DEHYDROGENASE 2, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0520">NAD</keyword>
    <keyword evidence="3 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5 9" id="KW-0642">Proline metabolism</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="11">
        <location>
            <begin position="75"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU366016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU366030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR24871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="573" mass="63644" checksum="805DEAC2947127D2" modified="2009-07-28" version="1">MLSRALLIRQSKRCVAQLSHVKPPKQITNEPVKPFSNKDILDWDLLRASISKFNSASLDVPLVINGKRIYHNEGSRQFFQQTNPAKHSQVLANVTQASKQDVRDAIQAAKSAKEKWYKMPFYDRAAVFLKAADLISTKYRYDMLAATMLGQGKNVYQAEIDCITELADFFRFNVKYATELYNQQPCESSPGVWNKAEYRPLEGFVYAVTPFNFTAISGNLIGAPALMGNTVVWKPSQAASLSNFLLLTVLEEAGLPHGVVNFVPGNPVDITNEVLADEEFSALHFTGSTAVFKQLYGKIQSGVVNNLYRDYPRIVGETGGKNFHLVHPSASLPHAVLSTLRGAFEYQGQKCSAASRLYLPKSQSTEFLENLVGTLESVKTVNTSASKINGGDLHGFVGPVIHQQSFEKLANAIEEAKKDPELEILCGGKYDKSNGWFVEPTIIKTTNPLHKFMSTEFFGPVLTVYEYPDSEFSQICETIDKTTAYGLTGAVFARDREAIILADEKLKYSAGNFYINDKCTGAVVGQQWFGGARMSGTDDKAGGSNILSRFVSIRNVKENFYELNDFKYPSNYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DM78</accession>
    <name>C5DM78_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Exosome complex component RRP45</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Ribosomal RNA-processing protein 45</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0G06644g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase PH family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555326.1">
        <property type="nucleotide sequence ID" value="XM_002555280.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DM78"/>
    <dbReference type="FunCoup" id="C5DM78">
        <property type="interactions" value="997"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DM78"/>
    <dbReference type="GeneID" id="8293595"/>
    <dbReference type="KEGG" id="lth:KLTH0G06644g"/>
    <dbReference type="eggNOG" id="KOG1614">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038194_0_0_1"/>
    <dbReference type="InParanoid" id="C5DM78"/>
    <dbReference type="OMA" id="GPQFENG"/>
    <dbReference type="OrthoDB" id="10264038at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035925">
        <property type="term" value="F:mRNA 3'-UTR AU-rich region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071028">
        <property type="term" value="P:nuclear mRNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034473">
        <property type="term" value="P:U1 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034476">
        <property type="term" value="P:U5 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11368">
        <property type="entry name" value="RNase_PH_RRP45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.70:FF:000005">
        <property type="entry name" value="Exosome complex component RRP45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015847">
        <property type="entry name" value="ExoRNase_PH_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050590">
        <property type="entry name" value="Exosome_comp_Rrp42_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033100">
        <property type="entry name" value="Rrp45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11097:SF14">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11097">
        <property type="entry name" value="EXOSOME COMPLEX EXONUCLEASE RIBOSOMAL RNA PROCESSING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03725">
        <property type="entry name" value="RNase_PH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="Ribonuclease PH domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0271">Exosome</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="11">
        <location>
            <begin position="32"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="12">
        <location>
            <begin position="191"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00077933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF03725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR24889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="305" mass="33566" checksum="2CE60748CCED4EE2" modified="2009-07-28" version="1">MAKEVEIPAAESKFVIECLRQNLRLDSRKFDEFRSVEIELGDDYGDVTVTMGKTKVHCRISCTITQPYEDRPFEGLFFISTETSPMAGPQFENGNNTGEDEVLCSRIIEKAVRRSGALDVEGLCIVAGSKCWAVRADVHFLNCDGGFIDASCIAVMVGLLHFKKPDVSVSGEQVTIHPVDEREPVPLGVLHVPICVSFSFFNPEDAEENIKGESNSEICIIDATLKEELLRDGTLTVTLNKNREVVQVSKAGGLPMDALTLMECCHKAYKITEAITDQINNVLKADATARNQYAAILSSENARES</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMA9</accession>
    <name>C5DMA9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0G07348p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0G07348g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555357.1">
        <property type="nucleotide sequence ID" value="XM_002555311.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DMA9">
        <property type="interactions" value="147"/>
    </dbReference>
    <dbReference type="GeneID" id="8293627"/>
    <dbReference type="KEGG" id="lth:KLTH0G07348g"/>
    <dbReference type="eggNOG" id="ENOG502QYHN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004492_1_0_1"/>
    <dbReference type="InParanoid" id="C5DMA9"/>
    <dbReference type="OMA" id="TNTYFND"/>
    <dbReference type="OrthoDB" id="8436363at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006913">
        <property type="term" value="P:nucleocytoplasmic transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027038">
        <property type="entry name" value="RanGap"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24113">
        <property type="entry name" value="RAN GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24113:SF12">
        <property type="entry name" value="RAN GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="35"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="237"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="401"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="450"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1243"/>
            <end position="1262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1275"/>
            <end position="1304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="118"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="136"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="237"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="271"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="324"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="360"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="401"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="466"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR24920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1321" mass="146522" checksum="2304F0757D208EE1" modified="2009-07-28" version="1">MSGTLDSPDTLDIEKPVKKRFSDSLHVDWLSRNSYVNGTPEAQVEEFKKTGDMGKQAPKAKDPVPEGNSNSRRRSDADDTLESRVLGPNRILRERRRSVSSQNEFGVAPSYSALNPYDTGTTIRRTKSLSMNSNGHIEEPKREKGGFFRSLFGRRKKDDSKSTNTNTKPALRRSSFSAPIKEVEPPNPPRSAPANIGEDSVSVTRDGANMPPVRRSKTAPISTENVEVRLDEFLQHCREHPLDSDTKKSSSSRATRTSKPEIPRHKATFSIDDNLSPTEPSSETQTHFDAKGRPIPPHPSKSDLLPALKRSERTQHHKGKLKRTNTNTSSTTNKFGAFLKRVTSHTEEHSHSSKQSLTDSESDSSDEDLPQISEDQRPAFIPGLEDIKPLKKVSFATNTYFNDPPQQICSKNPRKGEVEVKSDGSVIIHRLTPEEKREILQKTSSGIVVGGSGHLKLLSDPSVSEEEAKRNEEKKPSVPKEDGSQVLNNEEDVGRGGQGGDSVAHENNQDTEEDVKVSKSASEVTIDKPMVSRRSMSSLGSSITVNEVEQETPFPPTNMKIPHDIVYTRCCHLREILPIPATLKQLKKGSTDPIPLLQMRNPKPSLIEVLSFSDFLSISPVLCLSLDGVNLSIKMLKTILCSLMHKKEFEKLTLRNTPLDHEGWRILCYFVSQCESLNSLDLTMVPGIAINVQKPSKSSLKSRDIRMTCDMNDRKNMNWNLLAASLVSGNGLEEIIASGAKMPLKEFKNFIELGCAHTQRLGLAYNELSLDQCCVLAEWLAQSKVTGIDIGFNDLRGKLGPFSATVINKMRQTKNIFKYISLNCTNLEVSENSSSENSDVLKLISVLAYCEDLKLLDISNNPKLFPHVTNHLTNYLPVFVNLIRLHMDNNSIPSTSIVEIAEVLPTCQKLTHVSIRGSKLDYASGCALVAAMRKSSSLLTLDLDCDNLPNKIRDKISLYSMKNMEAALNKVDKSTSPEMSDTLACLQKELSDLLTEKPVKQEEIQVAAQSFLHRLSRARSFINKVTEDLFKLRVEGNLSTEGKDTLIRFCFIDATFERGLKLLAQRYSNLSFSSGSLCANSKTGTDAGPEMRASGLPLKKETSSATLSSKHFSDSGHAALLPFHYPPIESSDPADDEVEIKWDSPDTEDQHARDQLREEGRILRGTRGIMGRVQSASNQGTSDDRECHITLDQKALRKAASELDSEQIKEFILSNDISSVAGVLEDLKSRGISLNDIFKKRHDRSDSNNQASVDCEKSSNSDNAVVGVQANLEEEKQRLANSGPALDSESSSEDEREGEAIDRVYDEVLDNIQRVRTNTES</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMN8</accession>
    <name>C5DMN8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">KLTH0G10406p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0G10406g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NFX1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555486.1">
        <property type="nucleotide sequence ID" value="XM_002555440.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMN8"/>
    <dbReference type="FunCoup" id="C5DMN8">
        <property type="interactions" value="1060"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMN8"/>
    <dbReference type="GeneID" id="8293764"/>
    <dbReference type="KEGG" id="lth:KLTH0G10406g"/>
    <dbReference type="eggNOG" id="KOG1952">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005714_2_2_1"/>
    <dbReference type="InParanoid" id="C5DMN8"/>
    <dbReference type="OMA" id="CPHPCDS"/>
    <dbReference type="OrthoDB" id="6512771at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06008">
        <property type="entry name" value="NF-X1-zinc-finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="CDD" id="cd06006">
        <property type="entry name" value="R3H_unknown_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.50">
        <property type="entry name" value="R3H-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034078">
        <property type="entry name" value="NFX1_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001374">
        <property type="entry name" value="R3H_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036867">
        <property type="entry name" value="R3H_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034077">
        <property type="entry name" value="R3H_FAP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000967">
        <property type="entry name" value="Znf_NFX1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12360">
        <property type="entry name" value="NUCLEAR TRANSCRIPTION FACTOR, X-BOX BINDING 1 NFX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12360:SF12">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR NF-X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01424">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01422">
        <property type="entry name" value="zf-NF-X1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00393">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00438">
        <property type="entry name" value="ZnF_NFX"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82708">
        <property type="entry name" value="R3H domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51061">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="12">
        <location>
            <begin position="79"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="R3H" evidence="13">
        <location>
            <begin position="733"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="24"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="25"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR25049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="963" mass="107549" checksum="B90FEEE6493BBD0B" modified="2009-07-28" version="1">MTSGQDQIAEAEFDEGYVVVRADSETSEAFENVGEDLDEQDEQDEQDEQDELDEQGDTYDQPYYEKTIKEIAKGDRYPCMICTVEMDFTCHMYACPECYRVFDYECIREWAVKSTQKSVTKTWKCPNCYFEKKDVPLKNRPTCWCGKNIHPEPNPLNPNSCGQTCDAPICVHGCSSTCHLGPHPTCMRMVQTKCRCGKKSKDVFCSEARKHQELFRCGNPCGLTLPCGVHKCQRECHNGVCGECPETIAKEINCYCGLESLPSIKCQDVKIQSKSQDGSGKKWIGAFACSRIRTVEYSCREHSFAEPCKAPPSIAGRIPCPYSPKALKTCPCGETPLENMETPRTKCTDPIPTCDATCGKLLSCGRHRCPFKCHTGTCMEICTCSDKIKCSCNSRPFIVPCKFQGPPRCNTKCESLMSCRRHRCAERCCDGRSLAQVREKKVFLTRDKLDESLVEAQHICLKKCNLKLSCGRHFCQRKCHPGNCPPCLESDSNDLVCPCGKTVAPAPVRCGTVLPPCRNPCIKTLQGPLDCGHPPMPHACHSLDEPCPSCTAPVFKQCKCGKNKKVRTLCFQNNVSCGRVCGKQLVSCHHSCTKTCHREGECQSICKQVCGISRSNCSHICRFKCHTGSPCPDVPCNVTVEVKCGCGHRSSFATCAAHEGQDPADQRPLECTEDCAAARRRLELMEAFGMNTETVQPTEGIVDLAALAEKVTTFQELMLPFTEASLSIFAKQSNWCSQIEEHLNRLMSDISKPSLHFKPMKPPQRHFIHELAQAYKLYCESQDKEPKRSVYVKKTVDSRKPSLSLKEALPLYQSFKNAQKERKLKELERSTTTRILNYTSTGESSPPVAQINGLLIQKVFEGTDEKTIEACFADYLKHTLIKDPNYLKLDSGDVMVFAENYATASINVERDINRVVGHLDLIAKDSFLAEGVTVCRVEQVMAEETNRSDESSVAEAPEDPLFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMS0</accession>
    <name>C5DMS0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0G11154p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0G11154g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555518.1">
        <property type="nucleotide sequence ID" value="XM_002555472.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMS0"/>
    <dbReference type="GeneID" id="8293797"/>
    <dbReference type="KEGG" id="lth:KLTH0G11154g"/>
    <dbReference type="eggNOG" id="KOG0254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_30_5_1"/>
    <dbReference type="InParanoid" id="C5DMS0"/>
    <dbReference type="OMA" id="MAIIVSC"/>
    <dbReference type="OrthoDB" id="6339427at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015798">
        <property type="term" value="P:myo-inositol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015791">
        <property type="term" value="P:polyol transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000134">
        <property type="entry name" value="MFS sugar transporter protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050814">
        <property type="entry name" value="Myo-inositol_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020:SF9">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="97"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="135"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="168"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="193"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="226"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="255"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="346"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="384"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="412"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="445"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="474"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="513"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="102"/>
            <end position="536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR25081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="572" mass="63786" checksum="E8808B40E9F66743" modified="2009-07-28" version="1">MSSSSRSIELNSVNNFSRDELKPVTKHDEDVFVDSSAAKIDIEQDAEKAHSVLNRQFNLDAEVAQLNELGDSFKQERSLWRRICSLDLGFEFEDKRYMVYMLGAFASAAGILSGIDQSIISGASIGMNKALHLSAHEASMVSSLMPLGAMAGSVIMSPLNEWFGRKSSIMISCIWYTVGAILCAASTSHHLMYAGRFILGVGVGIEGGCVGIYVSESVPSTVRGNLVSMYQFNIALGELFGYIVGVIFFDVHGGWRFMVGSSLVFSTILLVGMFFLPESPRWLVHKGRIGEGWNVWKRLRDVKHDNNNLEFLEMRHAAAQDKELRANESRFQAWFDLIRIPRNRRALVYAVMMVSLGQLTGINAIMYYMSTLMGQIGFSEKRSVAMSMVGGAALLLGTIPAILYMDRFGRRTWANTIVLFTLGLVLVGVGYQINRETNLPAAEGVYLTGQILYNMAFGSYSALTWVLPSESFSLATRSVGMTVCSTMLYLWSWTVTYNFDRMQKAMTYTGLTLGFYGGIAIVIGIPYQLLFMPETKNKTLEEIDDIFSRPTIDIVRENAANVKRNFARVFRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMZ8</accession>
    <name>C5DMZ8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0G13002p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0G13002g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555596.1">
        <property type="nucleotide sequence ID" value="XM_002555550.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMZ8"/>
    <dbReference type="FunCoup" id="C5DMZ8">
        <property type="interactions" value="248"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMZ8"/>
    <dbReference type="GeneID" id="8293879"/>
    <dbReference type="KEGG" id="lth:KLTH0G13002g"/>
    <dbReference type="eggNOG" id="ENOG502S206">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077720_0_0_1"/>
    <dbReference type="InParanoid" id="C5DMZ8"/>
    <dbReference type="OMA" id="CYEEDNC"/>
    <dbReference type="OrthoDB" id="4061647at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR25159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="236" mass="27448" checksum="150D7B10E5751ABB" modified="2009-07-28" version="1">MPKKRLVSPEQPTFELPSLPPWRTPKIKILHHTPSRGRSLLDENDIFETPQEGDQIKKVEKNPDVYEYAPFCDKKLLANSKVTQLLNSETATHCLVFHKDEHRSPNKKYRPDLNMDCCNSSDEEDLPLDGSLLEPVTGPSCTSERPNIRRFENNASTERNALRDFWGGSEVPEILDRSQLRDLYTILQQESDLVHRSERQVQSQNQIPRQIEGDIYHWLRKYCNNTWEGGSWMFNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNA0</accession>
    <name>C5DNA0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0G15290p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0G15290g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555698.1">
        <property type="nucleotide sequence ID" value="XM_002555652.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNA0"/>
    <dbReference type="GeneID" id="8293983"/>
    <dbReference type="KEGG" id="lth:KLTH0G15290g"/>
    <dbReference type="HOGENOM" id="CLU_2333965_0_0_1"/>
    <dbReference type="InParanoid" id="C5DNA0"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950438" evidence="1">
        <location>
            <begin position="18"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR25261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="98" mass="10863" checksum="C44049DD25CC6FDC" modified="2009-07-28" version="1">MKSTLFIASTLLSLAAATFPTNITLVDFIKEYTLDQIALPVEDGADLWILSKDGDRQNVSTDSLLYTTWSESDWPLFEYGCHLETETGGSFAYSIGLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNC7</accession>
    <name>C5DNC7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA exonuclease 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0G15906g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Exoribonuclease involved in ribosome biosynthesis. Involved in the processing of ITS1, the internal transcribed spacer localized between the 18S and 5.8S rRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the REXO4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555725.1">
        <property type="nucleotide sequence ID" value="XM_002555679.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNC7"/>
    <dbReference type="FunCoup" id="C5DNC7">
        <property type="interactions" value="862"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNC7"/>
    <dbReference type="GeneID" id="8294011"/>
    <dbReference type="KEGG" id="lth:KLTH0G15906g"/>
    <dbReference type="eggNOG" id="KOG2249">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022453_2_0_1"/>
    <dbReference type="InParanoid" id="C5DNC7"/>
    <dbReference type="OMA" id="TMLIYKS"/>
    <dbReference type="OrthoDB" id="8191639at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06144">
        <property type="entry name" value="REX4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000007">
        <property type="entry name" value="Interferon-stimulated exonuclease gene 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037431">
        <property type="entry name" value="REX4_DEDDh_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047021">
        <property type="entry name" value="REXO1/3/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Ribonucl_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801:SF45">
        <property type="entry name" value="RNA EXONUCLEASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801">
        <property type="entry name" value="RNA EXONUCLEASE REXO1 / RECO3 FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Exonuclease" evidence="11">
        <location>
            <begin position="106"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="16"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR25288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="278" mass="31112" checksum="110664F6DCC7E406" modified="2009-07-28" version="1">MVLSSNWLKLQETQKVHKPSKGKVSKTTKRKDVISNSTANNKKGPKGKKNIMGMVHSMNHEIEKIKERKLQVNQTEKSKPLAAELEETLEADAAARSINRASEIGKYVAIDCEFVGVGPEGKDSALARATVVNYFGHVVLDVFVKPQEKVTDWRTWVSGVRPQDMKEAVPFSVAQAKVAKTLENRILVGHSVAHDLQSLFLSHPRSAIRDTSRHLPFRKQYAGGKTPSLKKLAKEILGIDIQGAEHSPIEDARATMLIYKSDRKEFERLHQIKFGTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNE0</accession>
    <name>C5DNE0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0G16192p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0G16192g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555738.1">
        <property type="nucleotide sequence ID" value="XM_002555692.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNE0"/>
    <dbReference type="FunCoup" id="C5DNE0">
        <property type="interactions" value="67"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNE0"/>
    <dbReference type="GeneID" id="8294024"/>
    <dbReference type="KEGG" id="lth:KLTH0G16192g"/>
    <dbReference type="eggNOG" id="ENOG502QW3C">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_364101_0_0_1"/>
    <dbReference type="InParanoid" id="C5DNE0"/>
    <dbReference type="OMA" id="CLSGTIW"/>
    <dbReference type="OrthoDB" id="2148946at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010972">
        <property type="term" value="P:negative regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052945">
        <property type="entry name" value="Mitotic_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006597">
        <property type="entry name" value="Sel1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43628">
        <property type="entry name" value="ACTIVATOR OF C KINASE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43628:SF11">
        <property type="entry name" value="PROTEIN DSF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08238">
        <property type="entry name" value="Sel1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00671">
        <property type="entry name" value="SEL1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="239"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="354"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="248"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="260"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="280"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="292"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="354"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="393"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR25301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="624" mass="69599" checksum="0547BE5B6F10D61B" modified="2009-07-28" version="1">MSFSTNKVSPLDGFRGESDKSTERNTRISDIMSMRDRNGSIVSFDSVKTTERLLDKLDLSAEDEMLLQQALKEDEARRKQPEAFQQRRVVCMPASGFPSLRKPGSSSGNSNLENCEPLMNIINELSEKDRIKLSKRGDVKLFLPKSRFSYLVEEESDGESNPFHEDNLCFNGRAINIEKFRLETYAEDPKMGSSSLETEERLPFDIVPERPTLKLHSKSSVQTPVGSLSSIETRYLIKPSTQSSTESETSRSVDGRNKISESPPSWKFSTPRKSTSSTTVNKVSLSPTKTPKSSHGKGHKKKPSFSFKNLFKSPRVDNESSPAKPARQQYDATPPTNRLEFSTLHLEEGAPQSFYTNAPASASTDSPRSLASSRRFLPHHSRAASDTKIRYASASQRSGSKDASLSKEFKPKHILHPIKTTTPELPLVRDGRPLSPDSRIRVAIDLRNRGFLKESAEHLKVLCEASNPTGYLLFGLALRYGSGIEKNYVESFSYLKKAAGIKDEDSEVLNVDMDPFRLKDVPHVSPEPLAPALHECGISYLKGYGIGVIDELKAIKYLELAASKGHVDSMCLSGTIWSKSSKIRKKNKARAAAWFRLADRRGADLIGADWIYKDKYQNASVYSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNG9</accession>
    <name>C5DNG9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0G16940p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0G16940g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oxidase-dependent Fe transporter (OFeT) (TC 9.A.10.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555767.1">
        <property type="nucleotide sequence ID" value="XM_002555721.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNG9"/>
    <dbReference type="FunCoup" id="C5DNG9">
        <property type="interactions" value="84"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNG9"/>
    <dbReference type="GeneID" id="8294058"/>
    <dbReference type="KEGG" id="lth:KLTH0G16940g"/>
    <dbReference type="eggNOG" id="ENOG502QQWE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046738_1_1_1"/>
    <dbReference type="InParanoid" id="C5DNG9"/>
    <dbReference type="OMA" id="SVWHLDC"/>
    <dbReference type="OrthoDB" id="4364at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033573">
        <property type="term" value="C:high-affinity iron permease complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015093">
        <property type="term" value="F:ferrous iron transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005217">
        <property type="entry name" value="EfeU/FTR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004923">
        <property type="entry name" value="FTR1/Fip1/EfeU"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00145">
        <property type="entry name" value="EfeU/Ftr1 family ferrous iron transporter subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31632">
        <property type="entry name" value="IRON TRANSPORTER FTH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31632:SF2">
        <property type="entry name" value="PLASMA MEMBRANE IRON PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03239">
        <property type="entry name" value="FTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0410">Iron transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="53"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="89"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="147"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="212"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="293"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="371"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="387"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR25330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="398" mass="44604" checksum="A3E2F138B3B1D78C" modified="2009-07-28" version="1">MASGDKVFNVSVFFVVFRECLEAVVIVSVLLSFLKQSIGTQDMNVYKKLRKQVWIGVASGLFICLAIGGGFIGAYYSLQNDIFGSAEDLWEGIFCMIATIMISVMGIAMLRINKMQAKWRVKIARALLDVPERKRDRFKIGFLSRKYAMFLLPFITVLREGLEAVVFVAGAGITTQGSSASAYPLPVVVGLIAGTLVGFLLYYGTSTSSMQVFLVASTCILYLISAGLFSRGAWYFENYRFNKASGGDASEGGDGNGSYNIRKAVYHVNCCNPELDNGWDIFNALLGWQNTGYLSSMLCYNIYWLVLIIVLALMMFEERRGHLPFCKNLTLRQLSPMYWIKNKKKQEISEREKDELFRRVEKLNFDEHGEIRHADNNVENSSSSSSAKQVDNVITVSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNL0</accession>
    <name>C5DNL0_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Sulfhydryl oxidase</fullName>
            <ecNumber evidence="8">1.8.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0G17908g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2</text>
            <dbReference type="Rhea" id="RHEA:17357"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:16520"/>
            <dbReference type="ChEBI" id="CHEBI:17412"/>
            <dbReference type="EC" id="1.8.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.8.3.2" evidence="8"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555808.1">
        <property type="nucleotide sequence ID" value="XM_002555762.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNL0"/>
    <dbReference type="FunCoup" id="C5DNL0">
        <property type="interactions" value="474"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNL0"/>
    <dbReference type="GeneID" id="8294102"/>
    <dbReference type="KEGG" id="lth:KLTH0G17908g"/>
    <dbReference type="eggNOG" id="KOG3355">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070631_1_0_1"/>
    <dbReference type="InParanoid" id="C5DNL0"/>
    <dbReference type="OMA" id="PCRTCNT"/>
    <dbReference type="OrthoDB" id="17199at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016971">
        <property type="term" value="F:flavin-dependent sulfhydryl oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.310:FF:000003">
        <property type="entry name" value="Sulfhydryl oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.320.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.310">
        <property type="entry name" value="ERV/ALR sulfhydryl oxidase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039799">
        <property type="entry name" value="ALR/ERV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036774">
        <property type="entry name" value="ERV/ALR_sulphydryl_oxid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017905">
        <property type="entry name" value="ERV/ALR_sulphydryl_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12645">
        <property type="entry name" value="ALR/ERV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12645:SF0">
        <property type="entry name" value="FAD-LINKED SULFHYDRYL OXIDASE ALR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04777">
        <property type="entry name" value="Evr1_Alr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69000">
        <property type="entry name" value="FAD-dependent thiol oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51324">
        <property type="entry name" value="ERV_ALR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 8" id="KW-0274">FAD</keyword>
    <keyword evidence="3 8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ERV/ALR sulfhydryl oxidase" evidence="9">
        <location>
            <begin position="75"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU371123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR25371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="180" mass="20557" checksum="B64A887DCCAC665E" modified="2009-07-28" version="1">MTGQRDDDSTKPKVGFSGRKIIYDEDGKPCRSCNSLLDFQLATGRISIPSPGKVPAAKMAAPAKQTTPASYPKVEPPDVEELGRSSWTLLHSIAAKYPQKPNEIQKGEMKQFMTIFSHVYPCWWCAKDFEKFIRENSPKVDSREELGRWMCEAHNSVNEKLGKKAFDCNLWEKRWKDGWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>C5E210</accession>
    <name>C5E210_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="16">KLTH0H01276p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">KLTH0H01276g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555933.1">
        <property type="nucleotide sequence ID" value="XM_002555887.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E210"/>
    <dbReference type="FunCoup" id="C5E210">
        <property type="interactions" value="191"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E210"/>
    <dbReference type="GeneID" id="8294246"/>
    <dbReference type="KEGG" id="lth:KLTH0H01276g"/>
    <dbReference type="eggNOG" id="KOG4198">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022834_0_0_1"/>
    <dbReference type="InParanoid" id="C5E210"/>
    <dbReference type="OMA" id="CTYHNFA"/>
    <dbReference type="OrthoDB" id="448399at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1060.10:FF:000021">
        <property type="entry name" value="MUTL protein homolog 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1060.10:FF:000024">
        <property type="entry name" value="RNA-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1060.10">
        <property type="entry name" value="Zinc finger, RanBP2-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036443">
        <property type="entry name" value="Znf_RanBP2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23111:SF40">
        <property type="entry name" value="RNA-BINDING PROTEIN INVOLVED IN HETEROCHROMATIN ASSEMBLY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23111">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00641">
        <property type="entry name" value="Zn_ribbon_RanBP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00547">
        <property type="entry name" value="ZnF_RBZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90209">
        <property type="entry name" value="Ran binding protein zinc finger-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50199">
        <property type="entry name" value="ZF_RANBP2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RRM" evidence="14">
        <location>
            <begin position="249"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="15">
        <location>
            <begin position="378"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="15">
        <location>
            <begin position="491"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="151"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="160"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="184"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAR30071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="557" mass="62572" checksum="C878758AA6698A4B" modified="2009-07-28" version="1">MHYVVLEVELAHREDSPKDWSQIAKFNYQFLDTETLEPVFEENKHVELGNGCTLQDAVAQLEKDISEHAKDDFVLCSLNSTWHFRVTIPRQARDEVFILPPHLQHPKVFDLWKEYDRWCMNHPETSAMRQNPLGKRTKDLAEMRSVLGLDGTEEGAHPDANQNGGEQSKAEQSGAEQLVAGQPDTKQPSTELSSSEQSPAEQPDTITQAVVIITQLHKKCVTADDKSLVLTHPYDSYLDVRNFLQERSKVLYMNNLPPDTTQSELESWFTQFGARPVGFWTVKNIVEETSNVNNNWSSNNSTYVEAQDSISGFVVFQSHEEATEALSLNGRSILSNIANTKQPRVIEHIVEIQPSSARVLDRAQEILSPFPQSKNKPRPGDWNCPSCGFSNFQRRTSCFRCSFPAASGAGMNNNGYMKNPLQPHNAQAGSQQQAAYKMRTPAGLQAMQVQSGPHMGQPQQQQQQQQRMRYNQGQRFGQGQQHQGGSNVPFRAGDWKCATCTYHNFAKNVVCLRCGGPKSTEHQQKELGTVNSYTDIQDIGNGMGISLNTRSFSEPAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2A5</accession>
    <name>C5E2A5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">KLTH0H03388p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0H03388g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Mitochondrial metal transporter involved in mitochondrial iron accumulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556028.1">
        <property type="nucleotide sequence ID" value="XM_002555982.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2A5"/>
    <dbReference type="FunCoup" id="C5E2A5">
        <property type="interactions" value="76"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2A5"/>
    <dbReference type="GeneID" id="8294341"/>
    <dbReference type="KEGG" id="lth:KLTH0H03388g"/>
    <dbReference type="eggNOG" id="KOG1485">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013430_12_2_1"/>
    <dbReference type="InParanoid" id="C5E2A5"/>
    <dbReference type="OMA" id="GVYFHSQ"/>
    <dbReference type="OrthoDB" id="435980at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1510.10:FF:000013">
        <property type="entry name" value="Cation efflux family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="entry name" value="Cation efflux protein transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058533">
        <property type="entry name" value="Cation_efflux_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050291">
        <property type="entry name" value="CDF_Transporter"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43840">
        <property type="entry name" value="MITOCHONDRIAL METAL TRANSPORTER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43840:SF15">
        <property type="entry name" value="MITOCHONDRIAL METAL TRANSPORTER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0409">Iron storage</keyword>
    <keyword evidence="5" id="KW-0410">Iron transport</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="12">
        <location>
            <begin position="135"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="458"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00055037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR30166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="501" mass="55030" checksum="BAD15A5DE86B7FFE" modified="2009-07-28" version="1">MIKPSAYPVGLQFYHNIGKSRCRQLVWNLCFHQRSIHNSSRRLINEGKKGGIGKTDAERRLEKQAKFQDLSDAFEESRHIHMKESETEQNDTLHLGSMLYREAGHTHSHASSQSNPLLKMSRKEFKKNPGVRITWIGLWINVGLALGKFAGAIMFHSQALIADSVHALSDLVSDFLTLASVKLSSKQPTAEYPGGYGKIETLGSLSVSAILAFAGLSIGWSSLCAIAAPLLPHAVLDVVSTFSHSHSHGGPTDVANINAAWIAGGSIIIKEWIFNATKKVAIETNSNVLLANAWHHRVDSLTSLVALVTISSGYFFNIQSLDAVGGLLVSALVVKTGADGMIGAMKELVDRAVAGDDPRYVTVKDNTQEILRKLISNNNAKKPYGLKNLTVLSSGPEMHAKMTLEVPLQRWENVLTIKEFEIVTDHLRKTLRENIPTLRNIDIDYVEERPPLSPEQLKEIENQKKLGQFPTPQNESASDGPLPSGHTHSHFGLGSGHTHKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2B8</accession>
    <name>C5E2B8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0H03674p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0H03674g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="CAR30179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_002555995.1"/>
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    <dbReference type="KEGG" id="lth:KLTH0H03674g"/>
    <dbReference type="eggNOG" id="KOG1674">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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    <dbReference type="OMA" id="RIQSKCM"/>
    <dbReference type="OrthoDB" id="5304883at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Cyclin-like_sf"/>
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        <property type="entry name" value="Cyclin_PHO80-like"/>
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    </dbReference>
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        <property type="entry name" value="PHO85 CYCLIN-10-RELATED"/>
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            <begin position="224"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAR30179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="484" mass="53427" checksum="DE1026A4096B0AB8" modified="2009-07-28" version="1">MSGLPSILDESLDRIARGETELDISESMNEDSLDLDDPVDDDTFELPLKPRRRVLHASNFADLEAEPALDKRVRFEAAGPPWSPLSVEKIAVPEKGIMNSKPPKLNPASSAGARKPHIEDLIQHAHSLNRYLDQNLDRINTFQSHLFEQGLEAPQNTGQILASNVATAPTTPSGSASNFQLSEIGDSSESLDYEQDIHDESISAAALQADSERDDRKIKQILSRHQTSTYPLSENQSSVNLAGDDSRSEVSALDHFRDLAASTRTSVATSSEDLFNMPHSPVIHDLKFIIPKIAAQRPSAISSLGAGDDAEPPDMDCEAALALYTTTITLLLMMSKQSEDPKQEALPSFATFVMESVPTLAYEDYLKRLHCKFSFAPIVYLTAAHLLQTLFLTRVDEELHCKHHLDPRQVHRLVIASIRLATKLLEDCVHSHSYFSRICGISKKLLTKLEVAFLECLNFEGLKITNQSLLDAAQVQQELSALAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2F9</accession>
    <name>C5E2F9_LACTC</name>
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        <submittedName>
            <fullName evidence="3">KLTH0H04620p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0H04620g</name>
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    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556082.1">
        <property type="nucleotide sequence ID" value="XM_002556036.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2F9"/>
    <dbReference type="GeneID" id="8294395"/>
    <dbReference type="KEGG" id="lth:KLTH0H04620g"/>
    <dbReference type="eggNOG" id="ENOG502S4UY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057557_0_0_1"/>
    <dbReference type="InParanoid" id="C5E2F9"/>
    <dbReference type="OMA" id="CCESRIS"/>
    <dbReference type="OrthoDB" id="4068945at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="375"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="177"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="183"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="214"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR30220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="401" mass="44502" checksum="BA45C9E322A4ECAD" modified="2009-07-28" version="1">MSFAMFNARDQTGCRRVVFEVRKTEGQGLQFPIGRASLKDPERRDREDNFMFCEKSLSKKHAILCIKRLVPSESDPFVPLLSQFRISVQDLGSTHGLVDLQSQDADASVIDLKNGERFGLIKLCQPVAPGQSRGAKLKFQVFIKTNESDDTHETLELVLRNVTHEWSPYVSRPATAAEEELEMPLSPSSCSTSSSSSDLNYSEDIDLDLGKYGSNESTEQGSQDEQAELSRMAEDCIETDSSHHTLFVSDEPACLADNAPTTYVRMPAPYEPLFAPRAECEGAYNRDETEMFDDKNTPEGTPECFDCSKSINPYNEDELCDSVRDLAEEDYSLTSRKRSFEPEKDQDVCLEKCKKAKTDIELDKGTEVGGSRRQVIIGSMMGFVAGSLGTLGLLVGIANMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2G1</accession>
    <name>C5E2G1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0H04664p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0H04664g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
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                <person name="Talla E."/>
            </authorList>
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            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556084.1">
        <property type="nucleotide sequence ID" value="XM_002556038.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2G1"/>
    <dbReference type="FunCoup" id="C5E2G1">
        <property type="interactions" value="391"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2G1"/>
    <dbReference type="GeneID" id="8294397"/>
    <dbReference type="KEGG" id="lth:KLTH0H04664g"/>
    <dbReference type="eggNOG" id="KOG0118">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016358_0_0_1"/>
    <dbReference type="InParanoid" id="C5E2G1"/>
    <dbReference type="OMA" id="NKITEMH"/>
    <dbReference type="OrthoDB" id="10044938at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052600">
        <property type="entry name" value="Nuc_rcpt_coact/corep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23295:SF6">
        <property type="entry name" value="NEOSIN, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23295">
        <property type="entry name" value="NUCLEAR RECEPTOR COACTIVATOR 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="362"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="312"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="434"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="587"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="730"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="38"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="53"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="70"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="81"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="189"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="201"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="232"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="589"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="631"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="658"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="763"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="785"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="801"/>
            <end position="813"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR30222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="813" mass="88692" checksum="F10666AC0A48DDAB" modified="2009-07-28" version="1">MSAEEPNSMDPTREDDLAPAALEGAPVEETNVLLADEVAGDDEEQEDDQESLYSHEEERDAAEKEDSATPEIQTATVQAENEGEVGAEGQDEEEDAQGGSDQVIEAVEIGDVQERKEEEEAQGPESTLIEPDVVTVGQPVDASADELDAAEASDSRERAEDDAEGSGQEAHPEEQTALVDFGSEDEKGQSVSSESGAQEQEASEDAGSDGDEKEQEQDADQPNHEAAESSEQESSSGTQSVEPQPFDVARYDMSSENVDYGLLQKQATYIVSSNMLNTPGFQQLVEPDKINAVLSFLNSNPETAFQAFAGQVAQPSNPQRASQHEPRPDLSQPMTAVERKRYNEYLRGENRITEIQNIPPKSRLFIGNLPLKNVAKDDLFRIFSPYGHIYQINIKNAFGFIQYDNPQSVKAAIAGESNELNFGKKLILEISSSNSRPQYDHGDHGTNSSSTFISSSKRPFEEEEEEDMYSDSSYKRGKRRVPDCQIYVKRTADRSYANEVFNRFKQASGLETDMIFLKPRMELRKMINDAAYDGVWGVVLVNKTRNVDIQTFYKGPQGETKFDEYASVSCEDAIAIFNNLKSNRGDRMAAPVQHAPQQHAPQPHAPQQHAPQQQYYGGYGAAPAPQPPHQGQPYIQQPPYGGAPQGPAPGYGGPPPMAQGYGAYPGAQAHVSPPPPVVAPPMAAGPQMDQQQLFSAIQNLPPNVVSSLLAMAQQQPQQQQQLLGIIQSMQPQAQPHGAQHGGPSVPPAYQGYHQMQSQPINSPPVQQHQQQPPAFAAKSPPAQNPIPQQSQAPASGNNNVQSLLDSLAQLQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2I9</accession>
    <name>C5E2I9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Imidazole glycerol phosphate synthase hisHF</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="12">Glutaminase</fullName>
                <ecNumber evidence="12">3.5.1.2</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="12">Cyclase</fullName>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">KLTH0H05368g</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10 12">IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The glutaminase domain produces the ammonia necessary for the cyclase domain to produce IGP and AICAR from PRFAR. The ammonia is channeled to the active site of the cyclase domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 12">
            <text>5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine = D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24793"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58278"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="ChEBI" id="CHEBI:58525"/>
            <dbReference type="EC" id="4.3.2.10"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 12">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 12">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 5/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the HisA/HisF family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11 12">In the C-terminal section; belongs to the HisA/HisF family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.2" evidence="12"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556112.1">
        <property type="nucleotide sequence ID" value="XM_002556066.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2I9"/>
    <dbReference type="FunCoup" id="C5E2I9">
        <property type="interactions" value="301"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2I9"/>
    <dbReference type="GeneID" id="8294427"/>
    <dbReference type="KEGG" id="lth:KLTH0H05368g"/>
    <dbReference type="eggNOG" id="KOG0623">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037550_0_0_1"/>
    <dbReference type="InParanoid" id="C5E2I9"/>
    <dbReference type="OMA" id="GNYGHFV"/>
    <dbReference type="OrthoDB" id="10254903at2759"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00010"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000107">
        <property type="term" value="F:imidazoleglycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01748">
        <property type="entry name" value="GATase1_IGP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04731">
        <property type="entry name" value="HisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000094">
        <property type="entry name" value="Imidazole glycerol phosphate synthase hisHF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000039">
        <property type="entry name" value="Imidazole glycerol phosphate synthase hisHF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00278">
        <property type="entry name" value="HisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006062">
        <property type="entry name" value="His_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004651">
        <property type="entry name" value="HisF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050064">
        <property type="entry name" value="IGPS_HisA/HisF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014640">
        <property type="entry name" value="IGPS_HisHF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010139">
        <property type="entry name" value="Imidazole-glycPsynth_HisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00735">
        <property type="entry name" value="hisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01855">
        <property type="entry name" value="IMP_synth_hisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21235:SF2">
        <property type="entry name" value="IMIDAZOLE GLYCEROL PHOSPHATE SYNTHASE HISHF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21235">
        <property type="entry name" value="IMIDAZOLE GLYCEROL PHOSPHATE SYNTHASE SUBUNIT HISF/H IGP SYNTHASE SUBUNIT HISF/H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00977">
        <property type="entry name" value="His_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036936">
        <property type="entry name" value="IGPS_HisHF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 12 15" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4 12" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="5 12 15" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="3 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 12" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase" evidence="16">
        <location>
            <begin position="6"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="PRFAR binding" evidence="14">
        <location>
            <begin position="362"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="PRFAR binding" evidence="14">
        <location>
            <begin position="400"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="PRFAR binding" evidence="14">
        <location>
            <begin position="472"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="PRFAR binding" evidence="14">
        <location>
            <begin position="498"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="PRFAR binding" evidence="14">
        <location>
            <begin position="521"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="active site" description="For GATase activity" evidence="13">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <feature type="active site" description="For GATase activity" evidence="13">
        <location>
            <position position="192"/>
        </location>
    </feature>
    <feature type="active site" description="For GATase activity" evidence="13">
        <location>
            <position position="194"/>
        </location>
    </feature>
    <feature type="active site" evidence="13">
        <location>
            <position position="243"/>
        </location>
    </feature>
    <feature type="active site" evidence="13">
        <location>
            <position position="402"/>
        </location>
    </feature>
    <feature type="binding site" description="covalent" evidence="14">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="467"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00055946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00061106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR036936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR036936-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR036936-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAR30250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="550" mass="60491" checksum="802B4983B39CC203" modified="2009-07-28" version="1">MSTVHIIDVESGNLKSLRNAVEFLGFNVKIVKSADDIELSKAERLILPGVGNFGHFVDNLTLKNFEKPIREYIASGKPIMGICVGLQCLFLGSEESPKSTGLKYLDLQLTKFDTSEKPVPEIGWNTCTPQDNLFGLDPCNRYYFVHSYAAIIDAEDKARLSEAGWNIAAAKYGSQEYIAAVARDNIFATQFHPEKSGHAGLEILKNFINQTHPTIKHTDREKAALQNDYSNYGLTRRIIACLDVRTNDQGDLVVTKGDQYDVREKQEGGNVRNLGKPVELAQKYYEQGADEVTFLNITSFRDCPLKDTPMLEVLKQAAKTVFVPLTVGGGIKDIVDVDGTHIPALQVAALYFASGADKVSIGSDAVYAAEKYYELGERGDGASPIETISKSYGAQAVVISVDPKRVYVDGPESVKHKTFETKFPNEKGQTWCWYQCTIKGGRESRDMGVWELTRACEALGAGEVLLNCIDKDGSNSGYDLELINHAKEAVKIPVIASSGAGEPKHFKEAFESTGADACLGAGMFHRGEYTVNDVKQYLLDSGFKVRMESQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2U2</accession>
    <name>C5E2U2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0H07722p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0H07722g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556215.1">
        <property type="nucleotide sequence ID" value="XM_002556169.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2U2"/>
    <dbReference type="FunCoup" id="C5E2U2">
        <property type="interactions" value="23"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2U2"/>
    <dbReference type="GeneID" id="8294533"/>
    <dbReference type="KEGG" id="lth:KLTH0H07722g"/>
    <dbReference type="eggNOG" id="KOG1572">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047845_4_0_1"/>
    <dbReference type="InParanoid" id="C5E2U2"/>
    <dbReference type="OMA" id="HYPCYIH"/>
    <dbReference type="OrthoDB" id="6375174at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000084">
        <property type="entry name" value="Tyrosine phospatase-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004861">
        <property type="entry name" value="Siw14-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126:SF14">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE OCA6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03162">
        <property type="entry name" value="Y_phosphatase2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="1">
        <location>
            <begin position="8"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR30353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="218" mass="25047" checksum="1B397C552F781E0E" modified="2009-07-28" version="1">MSLVTPLYFSAVQPNFYRGSCPRETNIPFLNTLGLKYILSLTPEPLTNDAVMSKFCAEKGIQAIHIECNNEKSQKDKSKTKVKRKKKPVPIEYDVVVRCVQFLIDRRHYPCYIHCTNGELVTSLVVACLRKLSYWSTVSIFNEYLTYTSSINIHERGFIENFNLEIEINDLEMKDKVPWITVRASDVADPHGGNTADKNIRRIQSFPGMLPRLKFHSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2Z7</accession>
    <name>C5E2Z7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0H09108p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0H09108g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556270.1">
        <property type="nucleotide sequence ID" value="XM_002556224.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2Z7"/>
    <dbReference type="FunCoup" id="C5E2Z7">
        <property type="interactions" value="135"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2Z7"/>
    <dbReference type="GeneID" id="8294595"/>
    <dbReference type="KEGG" id="lth:KLTH0H09108g"/>
    <dbReference type="eggNOG" id="ENOG502QWIZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_360944_0_0_1"/>
    <dbReference type="InParanoid" id="C5E2Z7"/>
    <dbReference type="OMA" id="NYILGGH"/>
    <dbReference type="OrthoDB" id="4069217at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054776">
        <property type="entry name" value="VIR1_yeast"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22575">
        <property type="entry name" value="Vir1p"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="717"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="718"/>
            <end position="734"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR30408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="794" mass="89030" checksum="B5D4FFA1F420372E" modified="2009-07-28" version="1">MVAAEMDALSRKRSESTDDPMAADMMLTVESSARILHSYPPSAAEALSAAYEFANAIRQLKPHQEPQVADLDVFTPLVQLCVSERDSKDILLDLLATTFAYCSVHRTLAKLVRVFVSRSLHNQPVASQLRELCGSFATDYKPGFYSVISKLECYDRLDASVFRELQLNLGPTMRMTWIPLWTQETGIDLYSAVLSHNLLVSTSAEPKLDFLIASWAHATTEWVGLKDRSNEALKSFIYQTARRTILFAKWIPGGFFKFCQQAIAEWIESDCLLKTETDVSFSLSVVMEVLDHPDLNFLEEPHLALLLKVALRRIWHMCSEGQVQTLHFELGKMGATQSLPGLLNITQYLTARFLLRVGSLAMSARNYNSNKEWMLQETSYQLPSFFDDIVPTIPPIPRSTFSFVNNTIESELLANRHLETVSSLLDSLKCVLSINKMVLRHYNASEIQVLALKSSNPQKDTTIDNRSSLEVLQLYFISMTSALLLSRQLTEEQYLMVAEGQYEYTIQGRLIRREALACFETLIGTFKGISLFQLVKFSARVSMAELSLQKVSMQTLNFVFFEASFSVTQVPSLTNKLTVQSLYEYINLWNDGSPIFRPFYTEMFRVPQPLVKCVQVELERFLCLLTFEVKKDSSAPTPTTSRGTESKNNGYVPKYNAHASSFIPASKAGSKPSMDIEKSFGTQASNQLIPLSADFPRQLSICHERTEDAGSAFAAQRYSGSADSTTPGSAQAPSPFSARRWDSNCFSQGLNNSPETSSAVVSTGKDYILGGHNKATNNSRAQSVHVDRFDYVRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5E303</accession>
    <name>C5E303_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0H09240p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0H09240g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HOP2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556276.1">
        <property type="nucleotide sequence ID" value="XM_002556230.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E303"/>
    <dbReference type="FunCoup" id="C5E303">
        <property type="interactions" value="248"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E303"/>
    <dbReference type="GeneID" id="8294601"/>
    <dbReference type="KEGG" id="lth:KLTH0H09240g"/>
    <dbReference type="eggNOG" id="ENOG502S1MU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063266_2_0_1"/>
    <dbReference type="InParanoid" id="C5E303"/>
    <dbReference type="OMA" id="RIICKLF"/>
    <dbReference type="OrthoDB" id="272266at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000794">
        <property type="term" value="C:condensed nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120231">
        <property type="term" value="C:DNA recombinase auxiliary factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120230">
        <property type="term" value="F:recombinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007129">
        <property type="term" value="P:homologous chromosome pairing at meiosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000709">
        <property type="term" value="P:meiotic joint molecule formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010774">
        <property type="term" value="P:meiotic strand invasion involved in reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010776">
        <property type="entry name" value="Hop2_WH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15938:SF0">
        <property type="entry name" value="HOMOLOGOUS-PAIRING PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15938">
        <property type="entry name" value="TBP-1 INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07106">
        <property type="entry name" value="WHD_TBPIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="5" id="KW-0469">Meiosis</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homologous-pairing protein 2 winged helix" evidence="7">
        <location>
            <begin position="17"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="98"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR30414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="214" mass="24209" checksum="6C4AF5C0B924CAB1" modified="2009-07-28" version="1">MSSKKKAEKAPQAKGAEAESIIESYLIEQYRPFAVNDIIQNLHNKVSRTNTIKALESLTKQQRITCKTFGKIAIYVCNEQELTSTNIGTGTDVSIETVTQLREELLETEKDRSEYSAQLQGVMRHPPNEELLAIVEAKKAEVLEVNNMLQNLLSDWKPENEELIQTVRAYEQKIGKELSARKKILNSAIALMKEALSLKNIDEFLVRVMPGTFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>C5E359</accession>
    <name>C5E359_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0H10604p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0H10604g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556332.1">
        <property type="nucleotide sequence ID" value="XM_002556286.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E359"/>
    <dbReference type="FunCoup" id="C5E359">
        <property type="interactions" value="50"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E359"/>
    <dbReference type="GeneID" id="8294663"/>
    <dbReference type="KEGG" id="lth:KLTH0H10604g"/>
    <dbReference type="eggNOG" id="ENOG502RZ40">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037144_0_0_1"/>
    <dbReference type="InParanoid" id="C5E359"/>
    <dbReference type="OrthoDB" id="2290221at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031932">
        <property type="term" value="C:TORC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038203">
        <property type="term" value="P:TORC2 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013745">
        <property type="entry name" value="Bit61/PRR5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32428">
        <property type="entry name" value="TARGET OF RAPAMYCIN COMPLEX 2 SUBUNIT BIT61-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32428:SF2">
        <property type="entry name" value="TARGET OF RAPAMYCIN COMPLEX 2 SUBUNIT BIT61-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08539">
        <property type="entry name" value="HbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="109"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR30470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="536" mass="59867" checksum="1434318B413A5E28" modified="2009-07-28" version="1">MNTSKKVMDNNVKRKRSFSALPIVHKLPGKNADSGRARFYSVSSQIVPQTIQVTDDLGRKAAATEPKSSSPALADLSKVGFQSIYRDPTVKKSQRSINTPEPSAKLHATSSSSSIKSSTITSNRSYRSGSNTSGLSHKRSALSLSNKLSGKKSVDTFDREKRKINGVDGSSSSISSSNKERSRLFTNTLTSAARKLFHRKDGRQEKRGEQLKSPTAVTSSTFGRFLHNKYSKHVGKATAHYKYSSGSFIDSAKSNANNPSTSNDVPLQLVQESNLDASDIQMLHDLIKNLKSLESNYRTFTVEELDALMSNVWGVYCSVVLTLFKNRELWELPAKIEDLNKILSFYIKLKTTSKNASASSKFLSEIEEFLTTCLYTLENQIVFNYSNENTINTALKRLCVIWEVFYQQIYHSTIALFLPLSNSFLVDTRYWSETFNGFSSDGIGNKRAGSLSLDFLLLKSFRDSIVSPYYQSFINSHEGASKGFHLYIMSEEEEKGVTQQDKLVLLQCFGILSSIRGTDIKQRVVDELLVGIRMSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3I3</accession>
    <name>C5E3I3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">1,4-alpha-glucan-branching enzyme</fullName>
            <ecNumber evidence="5">2.4.1.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Glycogen-branching enzyme</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0H13794g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Glycogen-branching enzyme participates in the glycogen biosynthetic process along with glycogenin and glycogen synthase. Generates alpha-1,6-glucosidic branches from alpha-1,4-linked glucose chains, to increase solubility of the glycogen polymer.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Transfers a segment of a (1-&gt;4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.</text>
            <dbReference type="EC" id="2.4.1.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Glycan biosynthesis; glycogen biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.18" evidence="5"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556456.1">
        <property type="nucleotide sequence ID" value="XM_002556410.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3I3"/>
    <dbReference type="FunCoup" id="C5E3I3">
        <property type="interactions" value="477"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3I3"/>
    <dbReference type="GeneID" id="8294817"/>
    <dbReference type="KEGG" id="lth:KLTH0H13794g"/>
    <dbReference type="eggNOG" id="KOG0470">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011131_2_2_1"/>
    <dbReference type="InParanoid" id="C5E3I3"/>
    <dbReference type="OMA" id="YEMHLGS"/>
    <dbReference type="OrthoDB" id="196493at2759"/>
    <dbReference type="UniPathway" id="UPA00164"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003844">
        <property type="term" value="F:1,4-alpha-glucan branching enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043169">
        <property type="term" value="F:cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd11321">
        <property type="entry name" value="AmyAc_bac_euk_BE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02854">
        <property type="entry name" value="E_set_GBE_euk_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000001">
        <property type="entry name" value="1,4-alpha-glucan branching enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001874">
        <property type="entry name" value="1,4-alpha-glucan-branching enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1180:FF:000003">
        <property type="entry name" value="1,4-alpha-glucan-branching enzyme, chloroplastic/amyloplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006048">
        <property type="entry name" value="A-amylase/branching_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037439">
        <property type="entry name" value="Branching_enzy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="GH13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004193">
        <property type="entry name" value="Glyco_hydro_13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43651">
        <property type="entry name" value="1,4-ALPHA-GLUCAN-BRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43651:SF3">
        <property type="entry name" value="1,4-ALPHA-GLUCAN-BRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02806">
        <property type="entry name" value="Alpha-amylase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02922">
        <property type="entry name" value="CBM_48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000463">
        <property type="entry name" value="GlgB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0320">Glycogen biosynthesis</keyword>
    <keyword evidence="8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 13 catalytic" evidence="14">
        <location>
            <begin position="210"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="13">
        <location>
            <position position="356"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="13">
        <location>
            <position position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00020932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000463-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR30594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="705" mass="80871" checksum="F7E20B5051CA94A7" modified="2009-07-28" version="1">MGNVPENVRPAVELDPWLEPFAAVLSERRELADKWAGDIRGSLGAGREHTLAAFARDGYQQFGLHADARTKEIRYREWAPNAQRAFLVGDFNGWNTESHELTQRDQYGVFSTTIAPLASGDFAIPHDSRIKVLLVLGDGTQLYRLPAWITRATQPDKETARQYGPSYEARFWNPPTQYEFRHARPRFSRDLDSLRIYEAHIGISSPEPKVASYRDFTANVLPKIRELGYDAIQLMAIMEHAYYASFGYQVTNFFAISSRYGTPEDLKELVDTAHGMGLLVLLDVVHSHASKNVEDGLNRFDGSDHQYFHSLLSRRGEHPLWDSRLFNYGSFEVLRFLLSNLAFYVDIYHFDGFRFDGVTSMLYNHHGVGAGGAFSGDYNEYLSKERSDVDHEALAYLMLANDLVRELLPESGVTIAEDVSGYPTLCLPRASGGAGFDYRLAMALPDMWIKLLKEKSDDDWEMGNIVHTLTNRRHGEKVVAYAESHDQALVGDKTLAFWLMDAAMYTDMTVLKPLTPVVDRGIALHKLIRLITHTLGGEAYLNFEGNEFGHPEWLDFPNVNNHDSYHYARRQFNLVDDHLLRYRHLYNFDKAMQLCERQFKWLNTPQAYISLKHEVDKVIAFERNGHLFIFNFHPTQSFADYRIGVERSGSYRIVLNSDRPEFGGHNRIDEDNSKFFTTDLRWNNRSNYIQVYIPTRTALILALDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3S2</accession>
    <name>C5E3S2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mitochondrial inner membrane protease subunit</fullName>
            <ecNumber evidence="9">3.4.21.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0H15884g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the peptidase S26 family. IMP1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.-" evidence="9"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556545.1">
        <property type="nucleotide sequence ID" value="XM_002556499.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3S2"/>
    <dbReference type="FunCoup" id="C5E3S2">
        <property type="interactions" value="415"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3S2"/>
    <dbReference type="GeneID" id="8294911"/>
    <dbReference type="KEGG" id="lth:KLTH0H15884g"/>
    <dbReference type="eggNOG" id="KOG0171">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028723_4_3_1"/>
    <dbReference type="InParanoid" id="C5E3S2"/>
    <dbReference type="OMA" id="LCKGPSM"/>
    <dbReference type="OrthoDB" id="308440at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042720">
        <property type="term" value="C:mitochondrial inner membrane peptidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006627">
        <property type="term" value="P:protein processing involved in protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06530">
        <property type="entry name" value="S26_SPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.109.10:FF:000018">
        <property type="entry name" value="Mitochondrial inner membrane protease subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.109.10">
        <property type="entry name" value="Umud Fragment, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052064">
        <property type="entry name" value="Mito_IMP1_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000223">
        <property type="entry name" value="Pept_S26A_signal_pept_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019757">
        <property type="entry name" value="Pept_S26A_signal_pept_1_Lys-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019756">
        <property type="entry name" value="Pept_S26A_signal_pept_1_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019533">
        <property type="entry name" value="Peptidase_S26"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02227">
        <property type="entry name" value="sigpep_I_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12383:SF16">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE PROTEASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12383">
        <property type="entry name" value="PROTEASE FAMILY S26 MITOCHONDRIAL INNER MEMBRANE PROTEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10502">
        <property type="entry name" value="Peptidase_S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00727">
        <property type="entry name" value="LEADERPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="LexA/Signal peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00501">
        <property type="entry name" value="SPASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00760">
        <property type="entry name" value="SPASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="2 9" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S26" evidence="10">
        <location>
            <begin position="13"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="39"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR600223-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF10502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR30683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="196" mass="21610" checksum="309742550C40856B" modified="2009-07-28" version="1">MRSGLSGWLKTGSFALRSFCLVHVIHNHFYEFTGTRGESMLPTLAATNDYVHALKLYRDGRGLTIGDCIVAAKPTDPYQRVCKRITGMPGDIILVDPSACVSNSPSSMDNRAGQNGEESLEAEPFNSFIKVPPGHVWVTGDNLAQSLDSRTYNSLPMGLIKGKIVAANNFNQPLWKNGRFLGFRKIENSYVDEVAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>C5DB93</accession>
    <name>C5DB93_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Transcription factor IIIA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0A00682g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551492.1">
        <property type="nucleotide sequence ID" value="XM_002551446.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DB93"/>
    <dbReference type="FunCoup" id="C5DB93">
        <property type="interactions" value="51"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DB93"/>
    <dbReference type="GeneID" id="8290283"/>
    <dbReference type="KEGG" id="lth:KLTH0A00682g"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044102_0_0_1"/>
    <dbReference type="InParanoid" id="C5DB93"/>
    <dbReference type="OMA" id="SFYKHPQ"/>
    <dbReference type="OrthoDB" id="4748970at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002391">
        <property type="entry name" value="Transcription factor IIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000912">
        <property type="entry name" value="Zinc finger protein 660"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051061">
        <property type="entry name" value="Zinc_finger_trans_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46179:SF13">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46179">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13894">
        <property type="entry name" value="zf-C2H2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="50"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="81"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="109"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="135"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="164"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="195"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="223"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="290"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="400"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="419"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR21050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="433" mass="49160" checksum="09BD5A357BD5A501" modified="2009-07-28" version="1">MEESEDHSNFWDVGKLGSEDGLPLVRSDSVGSIQSISSAGSTSSSRAKTWVCEYEGCSKAFTRPSQLTEHQETVHQGLKPFTCTECGRQFTRKTHLERHMFTHTAEKPFHCSHCGKGVTTRQQLRRHEITHTRSFVCPHEGCGESFYKHPQLRSHVLAVHLQKLNCEFCGKKFQRPYRLKNHIAKHHNPDTVYKYQCSHGSCVEAFKTWTALQQHVKEAHPKLQCPTCGKACVGEQGLQMHMRVHSSMTVIKNWKCESCPDLYFAKKADLVSHCLEHHKDLVSTLLNDNEREPAEPSATASPNNIPLESGARPSRLRSSSTKHDSESELESIQTEVKLRKYFDSGKSSVSLLLNSAGRKLKCPYLGCYRSFKTEEKYEMHIQKHKIHQLKLKILQEKNQATSLASEENELESNMERKNGNSQDLESLSAAANR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBC0</accession>
    <name>C5DBC0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0A01298p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0A01298g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551519.1">
        <property type="nucleotide sequence ID" value="XM_002551473.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBC0"/>
    <dbReference type="FunCoup" id="C5DBC0">
        <property type="interactions" value="265"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBC0"/>
    <dbReference type="GeneID" id="8290311"/>
    <dbReference type="KEGG" id="lth:KLTH0A01298g"/>
    <dbReference type="eggNOG" id="KOG3429">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089470_0_2_1"/>
    <dbReference type="InParanoid" id="C5DBC0"/>
    <dbReference type="OMA" id="GGQNVNC"/>
    <dbReference type="OrthoDB" id="270639at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004045">
        <property type="term" value="F:peptidyl-tRNA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016150">
        <property type="term" value="F:translation release factor activity, codon nonspecific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070126">
        <property type="term" value="P:mitochondrial translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052104">
        <property type="entry name" value="Mito_Release_Factor_mL62"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000352">
        <property type="entry name" value="Pep_chain_release_fac_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11075:SF54">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11075">
        <property type="entry name" value="PEPTIDE CHAIN RELEASE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00472">
        <property type="entry name" value="RF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110916">
        <property type="entry name" value="Peptidyl-tRNA hydrolase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950404" evidence="2">
        <location>
            <begin position="21"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="Prokaryotic-type class I peptide chain release factors" evidence="3">
        <location>
            <begin position="48"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="163"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="172"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR21077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="185" mass="21594" checksum="91B936E24647E148" modified="2009-07-28" version="1">MRFGCRSRLPSLFFMRSVAGLLPIVRQYSAKTSLPAQQWVDSLTVRALPEKAFSYNFDRSSGPGGQNVNKVNSKCTLTIHAFSKCDWIPQEVRDQLVQRRFRYLSSSRDCVVIQSDQSRSRETNRQICLEKLVSELKTTCWFPKEAQPEDLQRWSAIRRKTSQQRLEGKKIKSDKKKLRRKVTDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBC7</accession>
    <name>C5DBC7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ras modification protein ERF4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0A01452g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Interacts with ERF2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ERF4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551526.1">
        <property type="nucleotide sequence ID" value="XM_002551480.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBC7"/>
    <dbReference type="FunCoup" id="C5DBC7">
        <property type="interactions" value="59"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBC7"/>
    <dbReference type="GeneID" id="8290318"/>
    <dbReference type="KEGG" id="lth:KLTH0A01452g"/>
    <dbReference type="eggNOG" id="ENOG502S30T">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087349_0_0_1"/>
    <dbReference type="InParanoid" id="C5DBC7"/>
    <dbReference type="OMA" id="CITHFPN"/>
    <dbReference type="OrthoDB" id="5377273at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031211">
        <property type="term" value="C:endoplasmic reticulum palmitoyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019383">
        <property type="entry name" value="Golgin_A_7/ERF4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051371">
        <property type="entry name" value="Ras_palmitoyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13254">
        <property type="entry name" value="GOLGI AUTOANTIGEN, GOLGIN SUBFAMILY A, 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13254:SF0">
        <property type="entry name" value="GOLGIN SUBFAMILY A MEMBER 7_ERF4 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10256">
        <property type="entry name" value="Erf4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Golgin subfamily A member 7/ERF4" evidence="7">
        <location>
            <begin position="74"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF10256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR21084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="222" mass="25607" checksum="FDB507996F81E512" modified="2009-07-28" version="1">MNSALTSSNSKHVVPDDSDQPKALFFNYHEFSVTDYAEIGKECSEHEEELALCITHFPNTYAPLDSIRFKQTRIVRIPRRYEDNLDWPVFCTLLPGHEPAALVNQDGSLQFVPYGFYENQIYGLSSVSPLSSYLSPEEFKDIVETVNMYLRQGYDPNTWRNIISATFDVLTFNLWNCLMIRLVSSPLLKLENYVETLNKSKLFKNKNIRLISPRRSGFLSVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBE7</accession>
    <name>C5DBE7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 16">Galactose-1-phosphate uridylyltransferase</fullName>
            <ecNumber evidence="4 16">2.7.7.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ordered locus">KLTH0A01980g</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 16">
            <text>alpha-D-galactose 1-phosphate + UDP-alpha-D-glucose = alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose</text>
            <dbReference type="Rhea" id="RHEA:13989"/>
            <dbReference type="ChEBI" id="CHEBI:58336"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="EC" id="2.7.7.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="15">Binds 1 Fe cation per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="14">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 16">Carbohydrate metabolism; galactose metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the galactose-1-phosphate uridylyltransferase type 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.12" evidence="4 16"/>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551546.1">
        <property type="nucleotide sequence ID" value="XM_002551500.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBE7"/>
    <dbReference type="FunCoup" id="C5DBE7">
        <property type="interactions" value="430"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBE7"/>
    <dbReference type="GeneID" id="8290341"/>
    <dbReference type="KEGG" id="lth:KLTH0A01980g"/>
    <dbReference type="eggNOG" id="KOG2958">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029960_0_0_1"/>
    <dbReference type="InParanoid" id="C5DBE7"/>
    <dbReference type="OMA" id="CFENRGA"/>
    <dbReference type="OrthoDB" id="418412at2759"/>
    <dbReference type="UniPathway" id="UPA00214"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008108">
        <property type="term" value="F:UDP-glucose:hexose-1-phosphate uridylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033499">
        <property type="term" value="P:galactose catabolic process via UDP-galactose, Leloir pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00608">
        <property type="entry name" value="GalT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.428.10:FF:000001">
        <property type="entry name" value="Galactose-1-phosphate uridylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001937">
        <property type="entry name" value="GalP_UDPtransf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019779">
        <property type="entry name" value="GalP_UDPtransf1_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005850">
        <property type="entry name" value="GalP_Utransf_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005849">
        <property type="entry name" value="GalP_Utransf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00209">
        <property type="entry name" value="galT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008724">
        <property type="entry name" value="PRK11720.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11943">
        <property type="entry name" value="GALACTOSE-1-PHOSPHATE URIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11943:SF1">
        <property type="entry name" value="GALACTOSE-1-PHOSPHATE URIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02744">
        <property type="entry name" value="GalP_UDP_tr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01087">
        <property type="entry name" value="GalP_UDP_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000808">
        <property type="entry name" value="GalT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00117">
        <property type="entry name" value="GAL_P_UDP_TRANSF_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 16" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="10 16" id="KW-0299">Galactose metabolism</keyword>
    <keyword evidence="15" id="KW-0408">Iron</keyword>
    <keyword evidence="8 14 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 16" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 16" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 14" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Galactose-1-phosphate uridyl transferase N-terminal" evidence="17">
        <location>
            <begin position="7"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="Galactose-1-phosphate uridyl transferase C-terminal" evidence="18">
        <location>
            <begin position="199"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-UMP-histidine intermediate" evidence="12">
        <location>
            <position position="183"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="32"/>
            <end position="35"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="13">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="13">
        <location>
            <begin position="81"/>
            <end position="82"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="13">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="13">
        <location>
            <begin position="176"/>
            <end position="178"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="13">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="298"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="315"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="317"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="330"/>
            <end position="331"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="335"/>
            <end position="336"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="13">
        <location>
            <position position="342"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000808-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000808-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000808-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000808-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF01087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAR21104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="367" mass="42330" checksum="F04402246C142B06" modified="2009-07-28" version="1">MTVNSGNFDFTDNSHRRFNPLTNSWVLVSPHRAKRPWMGQQEVPFSPNEPEFDAKCYLCPGNTRASGEINPQYPSTFIFPNDFPAVKLNQPNFIDDGKEMTLKDRFMRAQQVEGKCFVICFSPKHNLTIPRMKIGELKNVVDAWAKLYLELEAEARTKATPYKYLQIFENKGTAMGCSNLHPHGQAWCLSTIPDEVDKEFRSFAKYSKEHSSCLLGDYIALELEERDRIVLQNDSFLVVVPYWAVWPFETLLMSKQHLCSLADFNDQQKLELASIIKKLTTKYDNLFETSFPYSMGLHQAPLNAIQQANNSSWFHMHFYPPLLRSATVRKFLVGFELLGEPQRDLTAEQAAKRLADLEEEVHFTEIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBE9</accession>
    <name>C5DBE9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0A02046p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0A02046g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551548.1">
        <property type="nucleotide sequence ID" value="XM_002551502.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBE9"/>
    <dbReference type="FunCoup" id="C5DBE9">
        <property type="interactions" value="1051"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBE9"/>
    <dbReference type="GeneID" id="8290344"/>
    <dbReference type="KEGG" id="lth:KLTH0A02046g"/>
    <dbReference type="eggNOG" id="KOG2026">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016848_2_1_1"/>
    <dbReference type="InParanoid" id="C5DBE9"/>
    <dbReference type="OMA" id="PQFLIFH"/>
    <dbReference type="OrthoDB" id="10263353at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF16">
        <property type="entry name" value="U4_U6.U5 TRI-SNRNP-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00290">
        <property type="entry name" value="ZnF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50271">
        <property type="entry name" value="ZF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBP-type" evidence="7">
        <location>
            <begin position="34"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="6">
        <location>
            <begin position="154"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR21106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="470" mass="54247" checksum="80C7EDFAECC5F7AA" modified="2009-07-28" version="1">MAPSRTIEMEKRARSPSQSSDGTPSKKISLAGAQVQPYLETVDVRKLDFDLEKLCSVTLSPLNVYCCLVCGRYLQGRGEKTVAFLHSIQEDHHVFINFVSLKTYLLPENREVQGVQLLETIKFAISPKYTQEDLLQFPRMCFDKSNTPYINGFIGLNDLGDNTHLSVIIQMMAHITPIRDFFLLESQSKIDDELIKRLALLIKKLWSPHILKAHLSPYELLQQLSRVNKNVNSDNRIKDPKSFMLWLINYMIAKAHPLKKVLTRNMRGEVEVRSIVVREKGTTGEKNLEFFEDEATKTIVLPKFWSLTLDLPPVSLFNEGSDTNSIPQVTLDSLLGKFSGHKKIPTREGMKQYKIVKYPRFLAINFDRFNWDAAMPIRNRNRTLVKFADALRFENKDYRLIANVTLYPVGHSRSGDEDVDDEARWKIQLSNPKNAEWFEIDGKNVTAKEKELLFLDEVYIQVWEMEQNKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBH6</accession>
    <name>C5DBH6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Structural maintenance of chromosomes protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0A02706g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SMC family. SMC1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551575.1">
        <property type="nucleotide sequence ID" value="XM_002551529.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBH6"/>
    <dbReference type="FunCoup" id="C5DBH6">
        <property type="interactions" value="832"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBH6"/>
    <dbReference type="GeneID" id="8290374"/>
    <dbReference type="KEGG" id="lth:KLTH0A02706g"/>
    <dbReference type="eggNOG" id="KOG0018">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001042_0_1_1"/>
    <dbReference type="InParanoid" id="C5DBH6"/>
    <dbReference type="OMA" id="KHMDFQR"/>
    <dbReference type="OrthoDB" id="5575062at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008278">
        <property type="term" value="C:cohesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007062">
        <property type="term" value="P:sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03275">
        <property type="entry name" value="ABC_SMC1_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1060.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024704">
        <property type="entry name" value="SMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028468">
        <property type="entry name" value="Smc1_ABC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010935">
        <property type="entry name" value="SMC_hinge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036277">
        <property type="entry name" value="SMC_hinge_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18937:SF12">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18937">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES SMC FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06470">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005719">
        <property type="entry name" value="SMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00968">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75553">
        <property type="entry name" value="Smc hinge domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMC hinge" evidence="12">
        <location>
            <begin position="531"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="179"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="253"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="323"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="440"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="714"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="815"/>
            <end position="933"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR005719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR21133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1228" mass="139658" checksum="3BA172C74B5D8CCB" modified="2009-07-28" version="1">MGRLLGIELYNFKSYKGKVSIGFGESNFTSVIGPNGSGKSNLMDAISFVLGMKSIHLRSHTLADLIYRGTLQDGDADSGYSETHDNENNPSSAYVKAFYSPSGQENDVAELTRTITLSQESTYKLNGETVSHKKYCDFLESENILIKARNFLVFQGDVEQVASQKPRELTTLFEQVSGSIQYKQEYDRLREELEKARSATSELIQSRKRANIGLKSFKEGVNKDEEYRKHLEERNKLQQQLIVWQLFHLQAKRDSLTESLKSSQRELQSLDSELNSEEQIVAKKKLSYAKKQTLAAKQRTRLSDKTKKVDHLTSSLLPIKSSKESIDKRLSAAVTKIESLQRDIDRQESLVKQYEHQLKVVNKAKTNFQAEIDESAKSSGDFKLNEANLKRYEDLKETFLSSGGSELEDKLLLEKNESQEISDEIELYSRQLDASRLKVNEELGAEKEALENEVSELTKELSGKTSQVSAAVKKLKELQSRAEYNNNREYEVSFKLKETLTKLDDMNATQRETAKERKLRENVSILRRLFPGVRGLVHELCRPKKEKYTVAVSTILGKSFDAVIVDHFSVAQQCVSFLKKQRSGILSFIPLDTIDVNKVSMMALDTKGCTLAIDAIDYERDLERAMQYVCSDSIICDSLAIAKDLKWRRNVRAKLVSLDGSIVHKAGLMTGGTTKSSGNRWDKEEYQGLMSLKDTLLGEISELSSANTIDSERIRSTENELSFLNSDVMSLRTQLNQVKRSLNEKETEIQYQQELIKGEFEPKIQSLKEAMTAHAKTTARFEEEKDRLLDSVYADFEGDIGFKVSQYEKHSGEMMRRQLEDLRKLQMQIAGIEKKLEFETERLQSTQERQQRVQVDIEKAKIKLEALNSDEQRITDEIQGVELTINEEQTRLDNLQNEINKDLVELNSSEETIQEATEHLQSAKRMHEALAEDIDKNSLEKANVLKNCKMLNIKLPDGSSSLERVPLDGVSDATIEAANAVFVNFTRLNRRLKQSGEAETGNELQRSIDELNELLTVLQPNSKAAGRYEEAKVKYESIFKETEKCKTKEKKVNEQFAKIRRLRKDAFESAFDHVSNAIDDVYRELTRDPHSTAELAGGNASLTLEDEDEPYLAGIRYHATPPTKRFKDMEFLSGGEKTIAALALLFAINSFQPSPFFVLDEVDAALDILNVERIATYIRQRALSNLQFIVISLKNTMFEKSQALVGVFRQQRDNTSRALTLNLENYED</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBT2</accession>
    <name>C5DBT2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Coatomer subunit epsilon</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0A05148g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. The coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">COPI-coated vesicle membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the COPE family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551681.1">
        <property type="nucleotide sequence ID" value="XM_002551635.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBT2"/>
    <dbReference type="FunCoup" id="C5DBT2">
        <property type="interactions" value="269"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBT2"/>
    <dbReference type="GeneID" id="8290485"/>
    <dbReference type="KEGG" id="lth:KLTH0A05148g"/>
    <dbReference type="eggNOG" id="KOG3081">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075638_0_0_1"/>
    <dbReference type="InParanoid" id="C5DBT2"/>
    <dbReference type="OMA" id="SNFYYFE"/>
    <dbReference type="OrthoDB" id="310217at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006822">
        <property type="entry name" value="Coatomer_esu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10805">
        <property type="entry name" value="COATOMER SUBUNIT EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10805:SF0">
        <property type="entry name" value="COATOMER SUBUNIT EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04733">
        <property type="entry name" value="Coatomer_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016478">
        <property type="entry name" value="Coatomer_esu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 11" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6 11" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8 11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR016478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR21239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="294" mass="32399" checksum="22E47F5A9848ECF0" modified="2009-07-28" version="1">MDLFTVKQQYYSGNYKQALQEIEKVASSDQDAVVFYRAKSLIALGEYEKESSQSGIATAFNTYADIVAGSGSLQDLESAVSSSKSAFSLNLLASAQAIQGQNEDAVKTCLEGLDSNETQGLAELVLLAVQIAVSDSSNRSESTASSILQNFLAAHEEYANEDEIIINLAESCVNFVAGREITGSNFYFYEELCQTLPSWKSQLGLMSLHLQQSHLPEAQAIVDLLESEYYQNQQESARLYTPQLLANKITLTAMQGGRVDELRSQLLELQPEHPLCQNYKINNAKFDEVVAKYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBW5</accession>
    <name>C5DBW5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">ATP-dependent 6-phosphofructokinase</fullName>
            <shortName evidence="17">ATP-PFK</shortName>
            <shortName evidence="17">Phosphofructokinase</shortName>
            <ecNumber evidence="17">2.7.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Phosphohexokinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ordered locus">KLTH0A05874g</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 17">Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>beta-D-fructose 6-phosphate + ATP = beta-D-fructose 1,6-bisphosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 17">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="17">Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5 17 18">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 17">Heterooctamer of 4 alpha and 4 beta chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 17">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Mitochondrion outer membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
            <orientation evidence="4">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade 'E' sub-subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.11" evidence="17"/>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551714.1">
        <property type="nucleotide sequence ID" value="XM_002551668.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBW5"/>
    <dbReference type="FunCoup" id="C5DBW5">
        <property type="interactions" value="1105"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBW5"/>
    <dbReference type="GeneID" id="8290518"/>
    <dbReference type="KEGG" id="lth:KLTH0A05874g"/>
    <dbReference type="eggNOG" id="KOG2440">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011053_0_0_1"/>
    <dbReference type="InParanoid" id="C5DBW5"/>
    <dbReference type="OMA" id="EWQDQMC"/>
    <dbReference type="OrthoDB" id="537915at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00182"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005945">
        <property type="term" value="C:6-phosphofructokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003872">
        <property type="term" value="F:6-phosphofructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070095">
        <property type="term" value="F:fructose-6-phosphate binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0048029">
        <property type="term" value="F:monosaccharide binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061621">
        <property type="term" value="P:canonical glycolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030388">
        <property type="term" value="P:fructose 1,6-bisphosphate metabolic process"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="ATP-dependent 6-phosphofructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.460:FF:000007">
        <property type="entry name" value="ATP-dependent 6-phosphofructokinase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ATP-dependent 6-phosphofructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.450">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="entry name" value="2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.460">
        <property type="entry name" value="Phosphofructokinase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03184">
        <property type="entry name" value="Phosphofructokinase_I_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009161">
        <property type="entry name" value="6-Pfructokinase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022953">
        <property type="entry name" value="ATP_PFK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015912">
        <property type="entry name" value="Phosphofructokinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000023">
        <property type="entry name" value="Phosphofructokinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035966">
        <property type="entry name" value="PKF_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02478">
        <property type="entry name" value="6PF1K_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13697:SF4">
        <property type="entry name" value="ATP-DEPENDENT 6-PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13697">
        <property type="entry name" value="PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00365">
        <property type="entry name" value="PFK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000533">
        <property type="entry name" value="ATP_PFK_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00476">
        <property type="entry name" value="PHFRCTKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53784">
        <property type="entry name" value="Phosphofructokinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00433">
        <property type="entry name" value="PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 17" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="13 17 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 17" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15 17 18" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="12 17 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="14 17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 17 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 17 18" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphofructokinase" evidence="19">
        <location>
            <begin position="247"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphofructokinase" evidence="19">
        <location>
            <begin position="636"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-terminal catalytic PFK domain 1" evidence="17">
        <location>
            <begin position="1"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interdomain linker" evidence="17">
        <location>
            <begin position="622"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="region of interest" description="C-terminal regulatory PFK domain 2" evidence="17">
        <location>
            <begin position="636"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="17">
        <location>
            <position position="396"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="318"/>
            <end position="319"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="348"/>
            <end position="351"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="349"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="17">
        <location>
            <begin position="394"/>
            <end position="396"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="431"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="17">
        <location>
            <begin position="438"/>
            <end position="440"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="17">
        <location>
            <position position="495"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="523"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="17">
        <location>
            <begin position="529"/>
            <end position="532"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="17">
        <location>
            <position position="706"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="17">
        <location>
            <begin position="764"/>
            <end position="768"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="17">
        <location>
            <begin position="809"/>
            <end position="811"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="895"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="17">
        <location>
            <begin position="901"/>
            <end position="904"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="17">
        <location>
            <position position="983"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRNR" id="PIRNR000533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CAR21272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1006" mass="109272" checksum="D8B65CA14A6B04C6" modified="2009-07-28" version="1">MRNSVSITSSFYDFIKDKLREVRPKRANLAQRRSKSNFSTSESIFLSIEMTVDIPLVNGTSECTVTAYSKDTYQSAVDFYAQFLQLEQESAAGTSTFLRNSNVALEVVLVEDSKKHAASEERLQKLTSSLETADWRSLVSDSLVFKAVDVAQCKANLEEQKASYQAYPTDLFPMELYAVDPVGNVVGVTKTKNAVSAMPTAPPASKGSFTSTAPSSAQSTIDLTSAGSMSISDSLTAEPKGSEQKAIAVMTSGGDAPGMNANVRAIVRTAISKGCRAYVVMEGYEGLVRGGPEYIKQFNWEDVRGWSSEGGTNIGTARCMEFRERAGRLLGALHLIEAGVDALIVCGGDGSLTGADLFRSEWPSLLEELRDTGKITSEQYTRYKHLNICGTVGSIDNDMSTTDATIGAYSSLDRICRAIDYIEATANSHSRAFVVEVMGRNCGWLALMAGIATTADYILIPEKPASSREWQDQMCDIVSKHRSRGKRTTIVVVAEGAIANDLTPISPNDVHKVLVDRLGLDTRVTTLGHVQRGGTAVAYDRILATLQGVEAVNAVLESSPDTPSPLIAINENKITRKSLVDSVRLTKSVAEAIHAKDFKKAMSLRDTEFVEHLNNFMAVNSADHNPPKLPQEKRLKIAIVNVGAPAGGINSAVYSMATYCMSQGHRPYAIYNGWSGLARHESVRSLDWKSMLGWQSRGGSEIGTNRVTPEEADIGMIAYYFQKYQFDGLMIVGGFEAFVSLHQLERARENYPAFRIPMVLIPATLSNNVPGTEYSLGTDTALNALMDYCDVIKQSAASTRGTAFVVDVQGGNSGFLATNASLAVGAQVSYVPEEGISLEQLSEDIETLRESFETAQGRNRFGKLILKSKNASKVFTPEVLAEVMTSEAKGSFSAKSAIPGHVQQGGLPSPIDRTRATRLAIKAVGYIEQQQEVIAKARADDEEFDPDNKEISATAAVLGIKGSHVVFSSIRQIYDFETEVVKRMPKVIHWESTRAIADSLVGRKKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>C5DC79</accession>
    <name>C5DC79_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0B00836p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0B00836g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid/polyamine transporter 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551828.1">
        <property type="nucleotide sequence ID" value="XM_002551782.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DC79"/>
    <dbReference type="STRING" id="559295.C5DC79"/>
    <dbReference type="GeneID" id="8290654"/>
    <dbReference type="KEGG" id="lth:KLTH0B00836g"/>
    <dbReference type="eggNOG" id="ENOG502QRQD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027816_0_1_1"/>
    <dbReference type="InParanoid" id="C5DC79"/>
    <dbReference type="OMA" id="WGVAYIR"/>
    <dbReference type="OrthoDB" id="294730at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000039">
        <property type="entry name" value="Neutral amino acid transporter (Eurofung)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF20">
        <property type="entry name" value="AMINO ACID TRANSPORTER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="66"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="93"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="141"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="172"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="205"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="247"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="286"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="328"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="369"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="402"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="443"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="9">
        <location>
            <begin position="63"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="10"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR21390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="490" mass="54679" checksum="80003149847C35A4" modified="2009-07-28" version="1">MSSNLSTAPEEGKKVYATRSRDSGTSESIAALPEAMQQTTKYSSETPLLILENESEHDIQYKTCSWVHTSGLMLSEYIVLAIMSFPWSYSVLGIVPGIILTLFVASTVLYTGLTIIDYCERFPHLKNVCDIGQHLFWGQKWAWYATLVCFLANNTLIQGLHVLVGAKYLNTVTDHSLCTVKFGVIVACISFVVSVPRTFSSLSKVAYFSAATMFIAVVLAMIFAGVQDHPYGYDGTPVTFRAFPEKGTTFVQGMGAFLNIVYTFVGQITYPQFISEMQRPAEFRKVLYIVTTCEIVIFTLAGVIIYVYVGNEYMSAPAFGSLTRTFKIIAFSFAVPTIIFAGSLYSNVSGRLLFFTFFKRSKHLYSHTTVGWLTWIGILALTWVGAFIIAEVVPFFSDLLSLMSSLFDCWFGFVFWGMAYFKLKEAKYQNPRYYSQLTASEKFHFFIAIFLIAVGLFILGPGLYAAIDSIILNYQDDAYGTVFSCASNGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5DC81</accession>
    <name>C5DC81_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0B00880p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0B00880g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551830.1">
        <property type="nucleotide sequence ID" value="XM_002551784.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DC81"/>
    <dbReference type="STRING" id="559295.C5DC81"/>
    <dbReference type="GeneID" id="8290656"/>
    <dbReference type="KEGG" id="lth:KLTH0B00880g"/>
    <dbReference type="HOGENOM" id="CLU_111217_0_0_1"/>
    <dbReference type="InParanoid" id="C5DC81"/>
    <dbReference type="OMA" id="KQTAQEH"/>
    <dbReference type="OrthoDB" id="1099063at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025715">
        <property type="entry name" value="FoP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13865">
        <property type="entry name" value="FoP_duplication"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01218">
        <property type="entry name" value="FoP_duplication"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Chromatin target of PRMT1 protein C-terminal" evidence="3">
        <location>
            <begin position="36"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="45"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR21392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="102" mass="11713" checksum="34DCCFB8E2F2FADC" modified="2009-07-28" version="1">MVKEIAEPVYSNIYDHESGRTAVFEFEAPEKTEEVHRALNEKEISGAKVTVEVFESRSRQSRRSQKKGHRGGRRGPRAPKREKPAQPTADQLDQELENYMNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>C5DC88</accession>
    <name>C5DC88_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0B01056p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0B01056g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MOG1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551837.1">
        <property type="nucleotide sequence ID" value="XM_002551791.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DC88"/>
    <dbReference type="FunCoup" id="C5DC88">
        <property type="interactions" value="308"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DC88"/>
    <dbReference type="GeneID" id="8290663"/>
    <dbReference type="KEGG" id="lth:KLTH0B01056g"/>
    <dbReference type="eggNOG" id="KOG3329">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081345_1_0_1"/>
    <dbReference type="InParanoid" id="C5DC88"/>
    <dbReference type="OMA" id="DVSHIRQ"/>
    <dbReference type="OrthoDB" id="10255285at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1000.10">
        <property type="entry name" value="Mog1/PsbP, alpha/beta/alpha sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007681">
        <property type="entry name" value="Mog1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016123">
        <property type="entry name" value="Mog1/PsbP_a/b/a-sand"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15837">
        <property type="entry name" value="RAN GUANINE NUCLEOTIDE RELEASE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15837:SF0">
        <property type="entry name" value="RAN GUANINE NUCLEOTIDE RELEASE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04603">
        <property type="entry name" value="Mog1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55724">
        <property type="entry name" value="Mog1p/PsbP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR21399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="200" mass="22196" checksum="712D15CAD8DBF63E" modified="2009-07-28" version="1">MFQKQALYGGAISTVIPKGFLDASILREVPDTQEVFVNSREEKEIAVFKDGLGLDESVIVDLLQRVEEDDSCRALDVHLQEIAGLNGSHEWNLVECEQSAPEVGQVPHLTCIAVEAAYKWGKSDMRETLVLCVALLRFDDVQTDVVLSVNIPVTSEPELQLLNAWLSKTSSTIPLRIAAAYHLVKQMASEFRILDKGLFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCA6</accession>
    <name>C5DCA6_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">C-1-tetrahydrofolate synthase, cytoplasmic</fullName>
            <ecNumber evidence="8">1.5.1.5</ecNumber>
            <ecNumber evidence="7">3.5.4.9</ecNumber>
            <ecNumber evidence="6">6.3.4.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="ordered locus">KLTH0B01496g</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>(6R)-5,10-methenyltetrahydrofolate + H2O = (6R)-10-formyltetrahydrofolate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
            <dbReference type="EC" id="3.5.4.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:23701"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="22">
            <dbReference type="Rhea" id="RHEA:23702"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NADP(+) = (6R)-5,10-methenyltetrahydrofolate + NADPH</text>
            <dbReference type="Rhea" id="RHEA:22812"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.5.1.5"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:22813"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="20">
            <dbReference type="Rhea" id="RHEA:22814"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>(6S)-5,6,7,8-tetrahydrofolate + formate + ATP = (6R)-10-formyltetrahydrofolate + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20221"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:20222"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="21">
            <dbReference type="Rhea" id="RHEA:20223"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the formate--tetrahydrofolate ligase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.5" evidence="8"/>
    <dbReference type="EC" id="3.5.4.9" evidence="7"/>
    <dbReference type="EC" id="6.3.4.3" evidence="6"/>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551855.1">
        <property type="nucleotide sequence ID" value="XM_002551809.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCA6"/>
    <dbReference type="STRING" id="559295.C5DCA6"/>
    <dbReference type="GeneID" id="8290683"/>
    <dbReference type="KEGG" id="lth:KLTH0B01496g"/>
    <dbReference type="eggNOG" id="KOG4230">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003601_2_0_1"/>
    <dbReference type="InParanoid" id="C5DCA6"/>
    <dbReference type="OMA" id="QPIMFRR"/>
    <dbReference type="OrthoDB" id="5126881at2759"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004329">
        <property type="term" value="F:formate-tetrahydrofolate ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004477">
        <property type="term" value="F:methenyltetrahydrofolate cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004488">
        <property type="term" value="F:methylenetetrahydrofolate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00477">
        <property type="entry name" value="FTHFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01080">
        <property type="entry name" value="NAD_bind_m-THF_DH_Cyclohyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000006">
        <property type="entry name" value="Bifunctional protein FolD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000245">
        <property type="entry name" value="C-1-tetrahydrofolate synthase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001123">
        <property type="entry name" value="C-1-tetrahydrofolate synthase, cytoplasmic isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10860:FF:000005">
        <property type="entry name" value="C-1-tetrahydrofolate synthase, cytoplasmic, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.410.10:FF:000001">
        <property type="entry name" value="Putative formate--tetrahydrofolate ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.410.10">
        <property type="entry name" value="Formyltetrahydrofolate synthetase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01543">
        <property type="entry name" value="FTHFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01576">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000559">
        <property type="entry name" value="Formate_THF_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020628">
        <property type="entry name" value="Formate_THF_ligase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000672">
        <property type="entry name" value="THF_DH/CycHdrlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020630">
        <property type="entry name" value="THF_DH/CycHdrlase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020867">
        <property type="entry name" value="THF_DH/CycHdrlase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020631">
        <property type="entry name" value="THF_DH/CycHdrlase_NAD-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099:SF5">
        <property type="entry name" value="C-1-TETRAHYDROFOLATE SYNTHASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099">
        <property type="entry name" value="C-1-TETRAHYDROFOLATE SYNTHASE, CYTOPLASMIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01268">
        <property type="entry name" value="FTHFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00763">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02882">
        <property type="entry name" value="THF_DHG_CYH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00085">
        <property type="entry name" value="THFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00721">
        <property type="entry name" value="FTHFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00722">
        <property type="entry name" value="FTHFS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00767">
        <property type="entry name" value="THF_DHG_CYH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0436">Ligase</keyword>
    <keyword evidence="19" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="17" id="KW-0521">NADP</keyword>
    <keyword evidence="13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tetrahydrofolate dehydrogenase/cyclohydrolase catalytic" evidence="23">
        <location>
            <begin position="6"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Tetrahydrofolate dehydrogenase/cyclohydrolase NAD(P)-binding" evidence="24">
        <location>
            <begin position="143"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00012859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00017592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00051435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00051544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00052163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF00763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF02882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="CAR21417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="945" mass="101590" checksum="D0D4A72DDFC8AD21" modified="2009-07-28" version="1">MVASLIDGKAIAKTIREEISQEIKTMSSGASGFRPTLAIFQVGNRPDSATYVRMKCKAAEDAGIEVRFVHLDEDVAEHTLLTQVQEMNEDSSVDGILVQLPLPGHIDEDKITSAVAARKDVDGFGAFNIGELNKKNGKPFFHPCTPEGIIELLVRNDVPIAGARALVIGRSDIVGSPVAELLKARDATVTVVHSKSKDIPDYVSKADIVVVAIGRPEFVKGAWFEGNSNCVVIDVGTNYVDDASKKSGYRCVGDVEFEAARQHVRLITPVPGGVGPMTVAMLMSNVFKAAKRSLEGEGKPAFEPLPLNLANPVPSDFEISRAQKPKEITQVAAEAGILPSELEPYGSTKAKVKLDILDRLGSRQNGKYVLVAGITPTPLGEGKSTTTVGLAQALGAHLKKTVFANVRQPSMGPTFGIKGGAAGGGYSQVIPMDEFNLHVTGDIHAISMANNLLAAAIDTRMFHEQTQKDAALYKRLVPSKRGVRKFTPTMLRRLEKLGINKEDPDSLTPEEITQFARLDIDPETITWKRVVDCNDRFLRGITIGEAPTERGFKRETGFDISVASECMAILALANSLSDMRERLGKIVIGASKSGVPVTCEDIGCAGAMTALLKDAVKPNIMQTLEGTPVFVHAGPFANISIGANSAISDKIALKLAGVDPTASEEVKKESQGYVVTEAGFDFTMGGERFLNIKCRSSGLVPDVVVIVATIRALKVHGGGPEVKADAPLPTEYLNENVDLLRKGCANLAKHITNVKAYNLPVVIAINKMSSDTEREHEVVREEALKAGADDALVSNHWEEGGKGAVDLAEGVIKACEQPNNKFKFLYETEGSSVEEKINTIAKEMYGAGSVEFLPEAQKKIDLYTKQGFSNLPICIAKTQYSLSHDANLKGVPTGFKFPIRDVRASVGAGYLYALAAEIQTIPGLPTHCGFMNIEVNEKGEIEGMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCB2</accession>
    <name>C5DCB2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Elongator complex protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0B01628g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the WD repeat ELP2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551861.1">
        <property type="nucleotide sequence ID" value="XM_002551815.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCB2"/>
    <dbReference type="FunCoup" id="C5DCB2">
        <property type="interactions" value="1196"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCB2"/>
    <dbReference type="GeneID" id="8290689"/>
    <dbReference type="KEGG" id="lth:KLTH0B01628g"/>
    <dbReference type="eggNOG" id="KOG1063">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006430_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCB2"/>
    <dbReference type="OMA" id="ENFRHIS"/>
    <dbReference type="OrthoDB" id="27911at2759"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033588">
        <property type="term" value="C:elongator holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000400">
        <property type="entry name" value="Elongator acetyltransferase complex subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037289">
        <property type="entry name" value="Elp2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44111">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44111:SF1">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 10" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="55"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="102"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="197"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="275"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="648"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="497"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="504"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR21423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="790" mass="88460" checksum="E76322AAAF15D467" modified="2009-07-28" version="1">MSGMAKAEAIFIGGNRQTQASDYNPKHGILAYGAGKTIAFWKPLDPAHRGVYATLKGHDAEVTCVRFVPGTEFMITASEDSQVRLWAFDGEDNSAMACIQSTDHHKHTITSLSVLSNLLTVGCADGSVSVWQINERQISLVHSFVLKAGVFPLCMALKSVEDSKVLLAVGGTNSNVFIYSFTREAAALENFQLAATLEGHEDWIKAMDFYEEGPGNMLLATGSQDRYIRLWRIRTNDLIDNSDEDDSKLKLLSNKQSKFYIDGSLRVAINFEALIIGHDDWVSCLKWHSDKLQLLASTADTAVMVWEPDAESGIWVCASRLGELSSKGASTATGSSGGFWSCNWFTQEKADYILTNGKTGSWRMWKSDDISGWEQQLAITGPTKPTTDVAWSPNGLYLLGTSLDQTTRLFARCKLDGEQDKISGSWFEFSRPQIHGYDMICVEPISNTRFISGGDEKIMRSFDEPKGVAQILQKFAGVPFENEKYMPESASLPALGLSSKATADVDEDEDEDPDARETNETKNISFDIVASLHSPPVEDQLQRHTLWPEIEKLYGHGYEISCLDVSPDAKIIASACRSNNLQHAVIRFFDSNTWLHLEPAPQFHNLTITRLRFSKDNTQLLSVSRDRQWAVWKRDLETNQFTLAYKNEKAHTRIIWDCEWAPLSFGSVFLTASRDKSIKLWRFSEEEQDYLQEASIKLGISVTAISVHEKLFDNKLIIAAGLENGSLQVYTYENEFQKVLEIEDRYTPADRISRLRWSSNGNELFLAAASADTSFRIYSVKSESIKNSPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCC9</accession>
    <name>C5DCC9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Eukaryotic translation initiation factor 2 subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0B02002g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF2B.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eIF-2-alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551878.1">
        <property type="nucleotide sequence ID" value="XM_002551832.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCC9"/>
    <dbReference type="FunCoup" id="C5DCC9">
        <property type="interactions" value="1407"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCC9"/>
    <dbReference type="GeneID" id="8290706"/>
    <dbReference type="KEGG" id="lth:KLTH0B02002g"/>
    <dbReference type="eggNOG" id="KOG2916">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033458_0_1_1"/>
    <dbReference type="InParanoid" id="C5DCC9"/>
    <dbReference type="OMA" id="DVNEHQR"/>
    <dbReference type="OrthoDB" id="1685042at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005850">
        <property type="term" value="C:eukaryotic translation initiation factor 2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04452">
        <property type="entry name" value="S1_IF2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1130:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000015">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.190:FF:000002">
        <property type="entry name" value="Translation initiation factor 2, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1130">
        <property type="entry name" value="EIF_2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.190">
        <property type="entry name" value="Translation initiation factor 2, subunit 1, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044126">
        <property type="entry name" value="S1_IF2_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024055">
        <property type="entry name" value="TIF2_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024054">
        <property type="entry name" value="TIF2_asu_middle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011488">
        <property type="entry name" value="TIF_2_asu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10602">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10602:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07541">
        <property type="entry name" value="EIF_2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110993">
        <property type="entry name" value="eIF-2-alpha, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116742">
        <property type="entry name" value="eIF2alpha middle domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="S1 motif" evidence="10">
        <location>
            <begin position="17"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00060206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR21440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="302" mass="34456" checksum="4CE351D6521F271C" modified="2009-07-28" version="1">MSTSNCRFYENKYPEVDDVVMVNVQQIAEMGAYVKLLEYDNIEGMVLLSELSRRRIRSIQKLIRVGRNEVVVVLRVDKEKGYIDLSKRRVSSEDIIKCEERYQKSKAVHSILRHCAEKFSFPLEDLYKSIAWPLSRKYGHAYDAFKLSIVDETVFEGIEPPSKEIFEELRLYISRRLTPQAVKIRADIEVSCFSYEGIDAIKAALKAAEEMSTEQMQIKAKLVAAPLYVLTTQALEKQHGIELLEKAIEKITKVITSHDGFCNITMAPKAVTATEDAELQALLENKALENGSDSEEEDSDYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCJ9</accession>
    <name>C5DCJ9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="10">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0B03652g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Ubiquitin ligase protein which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8 10">Belongs to the E3 ubiquitin-protein ligase UBR1-like family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="10"/>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551948.1">
        <property type="nucleotide sequence ID" value="XM_002551902.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="C5DCJ9">
        <property type="interactions" value="732"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCJ9"/>
    <dbReference type="GeneID" id="8290781"/>
    <dbReference type="KEGG" id="lth:KLTH0B03652g"/>
    <dbReference type="eggNOG" id="KOG1140">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_238060_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCJ9"/>
    <dbReference type="OMA" id="WANTISM"/>
    <dbReference type="OrthoDB" id="26387at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071596">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process via the N-end rule pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd19672">
        <property type="entry name" value="UBR-box_UBR1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.30:FF:000002">
        <property type="entry name" value="Putative e3 ubiquitin-protein ligase ubr3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044046">
        <property type="entry name" value="E3_ligase_UBR-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039164">
        <property type="entry name" value="UBR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055194">
        <property type="entry name" value="UBR1-like_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003126">
        <property type="entry name" value="Znf_UBR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497:SF26">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497">
        <property type="entry name" value="UBIQUITIN LIGASE E3 ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18995">
        <property type="entry name" value="PRT6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22960">
        <property type="entry name" value="WHD_UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02207">
        <property type="entry name" value="zf-UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00396">
        <property type="entry name" value="ZnF_UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51157">
        <property type="entry name" value="ZF_UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBR-type" evidence="12">
        <location>
            <begin position="111"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="UBR-type" evidence="9">
        <location>
            <begin position="111"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1798"/>
            <end position="1824"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1897"/>
            <end position="1923"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1802"/>
            <end position="1820"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1904"/>
            <end position="1913"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1914"/>
            <end position="1923"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU366018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR21510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1923" mass="220021" checksum="45F8669A8A87D62A" modified="2009-07-28" version="1">MIREGSADFQLHLRRTLQLIQNEPHFKKAKGPKDRAELDKLLKSFIFRYLYFIVSNNGKFLPQLFTSVPHEKFPATLQEALDVDRASISRHDPFYVIDDSVANESHRHTGRNCGRKFQAGEPIYRCKECSFDDTCVLCIHCFNAKDHINHHVYTSICTHVNNGICDCGDVEAWNVPLNCKAETEDEGSESEGAFESPEMTYILELTLTEIFDHFLDVFNQNIEPLPTMQRDITVKLRDMVQSEKSEEKEEFLQDLQYKNEDMQDLKKGLTEIEAPDYTVLVYNDEYHNYSQATAALKQGVPDDKNTDKLTARIDGEGRAMLKCSKDISSVLSGFFAVRTNGLSATLTAWSEYIQQEACKYIIAWVGHCLTIPNSSFQQGFRSAMGRVLCSGSERLPEAVDVSLLVDKHFPAKHSEEDPYRYANLSVLDRENVVPFSHHKVLAEGETDHISLTLNKTEPPSPKRYANSRLQLLLFFDNRYWKKLRKDIQDIVIPTMASSLEFKPIFCEQLVEIFNHMTRSVAFMDREPQLTVLRESVVQLFTCPTNAYSIFQSGSFCDIMWSVIDIFTEFSKKDAGVLVWQKVKKTNPTKSYSISFKQNLYSVETILSKVSDPNLLLRPKEFIAIVTLCKLFNGAWKIKRKEGEHVLREDQNFIPYLEYTTSIYSIVQTVDKILENKRDEVDETLLLQAIKLLSTFLGHRTPSQTVYDSHEIIKFKVSKQPVAFMNPVHTLMSFLIEKVPLAKAFAALSGCNDFLTISDFSLRAVVLCSQIDVGFWVRNGMSVLRQSSYYKNNPEMACYIRDVHLNQLTFLNEKDDLVRLIYNILDRWELLEWFNGKEDFDKTVYEDKIVYILQQFIAFVYQLLTERQSFKIFKSPEEKEYYQIKRAIMYGLYMEPLSYSSLLSAVPEYLTESTDQFDRALEDVSVYMGPKGLMDNGVFKLKPELYKRIDPLQLPNMGNDFEHSASIIKARLAESGEDPKNTVLEPQLISPKFLDESAKNLGAFTRTKVFAKVIYNLLRVSIDLDEGTFLYALLHLVHSILKDDELVNGKNSIPEVYISKPICNLLFIIIDIKSSVFSENVVAKADYLLESLIWKNPTSILESLVSSFGEECVERYKARKLHQGINFEESEKGRKRRLAKERQEKILSKFSRQQHKFMKENQSEFGSNDDDIEMADNGLSQAVEDYTCALCQDTSSVDAFVIPVYHENTPIFRNGDIRNVKEFGVEWNRFQNNKDSATTYEEESVQNFREDGSKGSRKVFVSCNHYIHYKCFKRYAQKKRFSTNSFICPLCQTFSNCVIPVAGYSPKPSESSIKELLQNSGAKSNIDHLHQTDPNEDFNGVFNMFLEINKKNMYYDRHWATTPESQRPDVAYILAVHWANTISMLEVSARIADHPNDSLLQGKEQKFRTLKNVLSSIAWLHKVLGRPSKEIIPYVNGEDTIWNQNQLFQFIVRRSLFSSEPITNVITGGLSVFSRQLLVDFLKGSTPNDVKMMREASANFGGIHDIDNETLDVLRDVCDISVSDEDTCRQLYSLAFASLIKNVLPTIRRCLILLKAINMLIMKSDEASLIVNGINLELVTSSSTPEYINSVIGLITPFSSFNDLLRKSNSYVKSVTDPYLSNIPYEFTGIVKLMDLAPHLNTYLTNSKEVKLREEHPARMRNAGNRLDFKICLTCGVKVHARNDRTELVRHLANHCFKPFGLFLIPNLNEVTLILTNPRSSISISAPYLNSHGEAGRNAIQRGDLTALSLQRYEHLNNVWLNSEVPGLISRVMGDEFRVLIVSNGVVLNFNRNVLRPRRANAEEPDGDSSSSEAEDDDLEGGEVRWEIRPEEFFEEAGIRFEGAGAIPGGDIRDFFQFINNLDTETDAFEEDEETGGEAQGLANAFVPRFEFLPNFRNFNARNEDEPDDEDEDEVNHPVSDDIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCM5</accession>
    <name>C5DCM5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0B04312p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0B04312g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551974.1">
        <property type="nucleotide sequence ID" value="XM_002551928.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCM5"/>
    <dbReference type="FunCoup" id="C5DCM5">
        <property type="interactions" value="197"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCM5"/>
    <dbReference type="GeneID" id="8290811"/>
    <dbReference type="KEGG" id="lth:KLTH0B04312g"/>
    <dbReference type="eggNOG" id="KOG0120">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033573_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCM5"/>
    <dbReference type="OMA" id="FIWQRPG"/>
    <dbReference type="OrthoDB" id="10266058at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12232">
        <property type="entry name" value="RRM3_U2AF65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23139">
        <property type="entry name" value="RNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="373"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="18"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="39"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="69"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR21536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="464" mass="52830" checksum="4E87F88635629B2B" modified="2009-07-28" version="1">MSNSREKSRFSAGPAGEYDSRSSYRRDTRGPRRFDRGDMGGTDGGRSRYGGRSSRNFENRSRYQGGGRYRQEPRYGDSRKPEDVDWSAVVSISQRRRMRETQWDMAPKGFEKVPAERAKLSGLFPLPGQPQDLDRSKLEGIVSSGVLTRRTRILFEDPTESNMSKTKYNQILILTSVGSSPAEMNQLAEYVKEFVKLVDETQELKEHKILQENRLYLRFSSEEITTIALSCQKFIERKTGSKFIWQRPGECVEKKEPVDPICAGSVIALQELGDCTEEGLQSDLNQSGVETNWLKLISLGKTNEFTGTALIDPKANVDELSHYKWIRPNDCKLRQDFTEITFQQLPHLVARKDHAESRVIVLLNCVDPMDLKNDEFVKEIRDSMTSSKTMTALGEIESVKIPQPGADYRTSFESIEESVGKIYVKFKEQESAKAAMSQLSGVSFNDRTVLCAYYSERDYNMDVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCM9</accession>
    <name>C5DCM9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0B04400p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0B04400g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551978.1">
        <property type="nucleotide sequence ID" value="XM_002551932.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCM9"/>
    <dbReference type="FunCoup" id="C5DCM9">
        <property type="interactions" value="153"/>
    </dbReference>
    <dbReference type="GeneID" id="8290815"/>
    <dbReference type="KEGG" id="lth:KLTH0B04400g"/>
    <dbReference type="eggNOG" id="ENOG502QVDR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041040_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCM9"/>
    <dbReference type="OMA" id="RCTPDRY"/>
    <dbReference type="OrthoDB" id="4084551at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028000">
        <property type="entry name" value="Pma1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14610">
        <property type="entry name" value="Psg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="345"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="422" mass="48924" checksum="F584E7C871198DDE" modified="2009-07-28" version="1">MSYPIIKFFIRNNNDFDLKIIPQRDFSEQNTMLVPQSIALLLLAWLSAVDARRNVIKPKETTTTSEIPKPWLRTIYSGQKEVVTPTVIAGVTFSAKPLATTNGLEPWISLDQFGSPKTKRPQIKGAKTKDARPDYSTYFKTVATRTLSYDELKAHNMEEDEVYEEEVFTDEDDTYVSLNPIIRCTPDRYFKKGPAGDISSEPFCTPKENKELKVDNTYFVSWYTKFFQKPDTDETIENVQLHLFYVKESIKDKGLKKRDLKAAFFSSGWLKNVDGIYPLELTADFLDGAYDRKVLLAIQPDNIRDDEFNPWEHYVLFRMIMGPRVAKTTKQQKALQDAGISDDTWYYVALSIPTVVVIACVAMYFFLYLNRSHRDIHNVKDFAINQRRRVLGKFKDFKKYKKINNRPYSELPTHTKKTGKQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCN5</accession>
    <name>C5DCN5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0B04532p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0B04532g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551984.1">
        <property type="nucleotide sequence ID" value="XM_002551938.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCN5"/>
    <dbReference type="GeneID" id="8290821"/>
    <dbReference type="KEGG" id="lth:KLTH0B04532g"/>
    <dbReference type="HOGENOM" id="CLU_2427406_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCN5"/>
    <dbReference type="OMA" id="ACHESVC"/>
    <dbReference type="OrthoDB" id="424753at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR21546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="110" mass="11523" checksum="9A4ABA527D1CFC54" modified="2009-07-28" version="1">MDAAGRLCSLLSCPTRAAPAMKRSSPDDCVYVASKKCQMYTACSGTDAFRAMLRTARRASRPAAPAAFEQECYYCSSRAPTAVCGACHESVCTVCSAGGTATCLNCRVAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCR4</accession>
    <name>C5DCR4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0B05192p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0B05192g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552013.1">
        <property type="nucleotide sequence ID" value="XM_002551967.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCR4"/>
    <dbReference type="GeneID" id="8290851"/>
    <dbReference type="KEGG" id="lth:KLTH0B05192g"/>
    <dbReference type="HOGENOM" id="CLU_1845451_0_0_1"/>
    <dbReference type="InParanoid" id="C5DCR4"/>
    <dbReference type="OrthoDB" id="10313024at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR21575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="139" mass="15864" checksum="67AF9251A40124CD" modified="2009-07-28" version="1">MSFELQILNDVLDWARAPGGPAGDYIHPSHVSLWSYPREKAFQVHEGNNNPSSARYGFDSPYGGLVTLESLYKERVDSSGKNYKIESGTSVENFTRKRKFEASAEDDFFHLQKDIGVRERSFKKPRGYACKNCCMYSHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCU4</accession>
    <name>C5DCU4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0B05874p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0B05874g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TBCB family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552043.1">
        <property type="nucleotide sequence ID" value="XM_002551997.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCU4"/>
    <dbReference type="FunCoup" id="C5DCU4">
        <property type="interactions" value="755"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCU4"/>
    <dbReference type="GeneID" id="8290882"/>
    <dbReference type="KEGG" id="lth:KLTH0B05874g"/>
    <dbReference type="eggNOG" id="KOG3206">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067577_2_0_1"/>
    <dbReference type="InParanoid" id="C5DCU4"/>
    <dbReference type="OMA" id="WCGIEFD"/>
    <dbReference type="OrthoDB" id="5295208at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035371">
        <property type="term" value="C:microtubule plus-end"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051010">
        <property type="term" value="F:microtubule plus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.190">
        <property type="entry name" value="CAP Gly-rich-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036859">
        <property type="entry name" value="CAP-Gly_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000938">
        <property type="entry name" value="CAP-Gly_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18916">
        <property type="entry name" value="DYNACTIN 1-RELATED MICROTUBULE-BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18916:SF85">
        <property type="entry name" value="TUBULIN-FOLDING COFACTOR B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01302">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14560">
        <property type="entry name" value="Ubiquitin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01052">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74924">
        <property type="entry name" value="Cap-Gly domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00845">
        <property type="entry name" value="CAP_GLY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50245">
        <property type="entry name" value="CAP_GLY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CAP-Gly" evidence="4">
        <location>
            <begin position="180"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR21605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="247" mass="28052" checksum="981E16632E40EC1E" modified="2009-07-28" version="1">MPQIQISSPLCSIVRELSPQWSWPQLCDKLSEITGVLPSDMQINVIFKDRTSLLINAPLTEQKPLSSLLSKNAVELQVNDSNASSLANELARDFTEGEQSSEQTYHLPEEDYQRRQDSVLQWKKDLQLGKFNPEYQARLQKDCEEQQSRAEELTINERCSVSTPNLPERRGWLRFVGSLTPLMDEPGIWCGVEFDEPVGKNDGSFKGRYFFGPVRPKYGAFVKPSTVKTSAKFSPLLDDIEFSDSEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>C5DD27</accession>
    <name>C5DD27_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0B07788g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="3"/>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552126.1">
        <property type="nucleotide sequence ID" value="XM_002552080.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DD27"/>
    <dbReference type="FunCoup" id="C5DD27">
        <property type="interactions" value="966"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DD27"/>
    <dbReference type="GeneID" id="8290966"/>
    <dbReference type="KEGG" id="lth:KLTH0B07788g"/>
    <dbReference type="eggNOG" id="KOG0789">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001645_9_1_1"/>
    <dbReference type="InParanoid" id="C5DD27"/>
    <dbReference type="OMA" id="WSIDKAP"/>
    <dbReference type="OrthoDB" id="10253954at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18533">
        <property type="entry name" value="PTP_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000115">
        <property type="entry name" value="Tyrosine-protein phosphatase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050348">
        <property type="entry name" value="Protein-Tyr_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016277">
        <property type="entry name" value="Ptp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134:SF449">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000938">
        <property type="entry name" value="PTPN1_yeast"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="7">
        <location>
            <begin position="17"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="8">
        <location>
            <begin position="229"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR21688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="332" mass="38126" checksum="3E547D79E671985B" modified="2009-07-28" version="1">MASETVPWYLKQSAQELVSKFRFIQHQEDLRLRDATLNPAKSRWSLGSSVDESNDDRNRYVNIMPYERNRVKLRTQAGNDYINASYVKVQVPGQSVRPGHYIATQGPTQNSWPQFWQMCYQQCTGSDIVIAMVTPLVEGGRQKCFEYWPHDAAAPLKIPRTQSSSCSASGPESSCTTFDVDLEVHFQCSERENDYTLSTLELAPADPAFPKKTVHHFYFDRWRDMSKPDEIVPILKLSRHAHSLNSSENPMVVHCSAGVGRTGTFITLDHLFHDTLDFTAAQPVQDYSHDLVEQIVLQLRTQRLKMVQMVDQFLFIYHAARYVFDLARTRDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>C5DD30</accession>
    <name>C5DD30_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0B07854p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0B07854g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552129.1">
        <property type="nucleotide sequence ID" value="XM_002552083.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DD30"/>
    <dbReference type="FunCoup" id="C5DD30">
        <property type="interactions" value="71"/>
    </dbReference>
    <dbReference type="GeneID" id="8290969"/>
    <dbReference type="KEGG" id="lth:KLTH0B07854g"/>
    <dbReference type="eggNOG" id="KOG2395">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007002_0_0_1"/>
    <dbReference type="InParanoid" id="C5DD30"/>
    <dbReference type="OMA" id="RLNETKW"/>
    <dbReference type="OrthoDB" id="10251113at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040458">
        <property type="entry name" value="Vid27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013863">
        <property type="entry name" value="VID27_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040979">
        <property type="entry name" value="Vid27_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040768">
        <property type="entry name" value="Vid27_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31913">
        <property type="entry name" value="VACUOLAR IMPORT AND DEGRADATION PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31913:SF0">
        <property type="entry name" value="VACUOLAR IMPORT AND DEGRADATION PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08553">
        <property type="entry name" value="VID27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17748">
        <property type="entry name" value="VID27_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17747">
        <property type="entry name" value="VID27_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69322">
        <property type="entry name" value="Tricorn protease domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Vid27 N-terminal" evidence="4">
        <location>
            <begin position="1"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="Vid27 PH-like" evidence="3">
        <location>
            <begin position="255"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="Vacuolar import/degradation Vid27 C-terminal" evidence="2">
        <location>
            <begin position="445"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="178"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="393"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="185"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="218"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="393"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF17747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF17748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR21691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="815" mass="92506" checksum="70764A25E5C13D7F" modified="2009-07-28" version="1">MNIIKKIMDSGSKPELVSIPAGQFFLLRPESSPKSTLECLYNDATLAIRESGSHRFELVVRKEVDDTELTSGDGGEDFEDDGLSVLSGQSKKEEEWSFNLDKSLHFRKSWSEQGEVAFVWRNTKGDAGEKFQFVVNSEIPLTDVGHFLHTVNVCEYEHKFKKQPSTATRENLRQFEFSHRHDEEAISDEDGDENDENNEDSQLGSKMGLLDLGNSSSESDDGFDSQEFEDAKDNFGSPKHPKQSVKVPNPPSAKTLLREHAALLVYDPTQQQFSLQDPDCGVSILDHGKFEYWLQINGKVLELGVDLTPLVNPFFQEETLCFIFNYSYETITLSYMLKFSDEATFSEFQQQWSKSLWEALNKQKWSAISEKEQRCISNANRDVDKELSLFLQDDDDSSSADSEESDENDEEEEEEEAEREIQSDVDSFDEDKKEEEYRSSTTLSGNKSLTVSYKNDRSFVVRGDKIGVFKVDDENNEVNFVTAINNVSSLKGRNFEPENPMLYTEDRAMIVQDKENPSLVFKMDLERGKVVEEWSAGGKDVLKYSHSKKFDQLTNEQTFLGVSGKSVFRLDPRISGANKLVTEQNKDYATNNKFSSLGTTQEGFLAVGSEKGEIRLFDKIGLRAKTLIPALGEPINHICNSADGKWLLATCDSSLLLIDLTIKEGKNAGSVGFVKSFSKEEMPNIFVLKIHPKTAAYMRTSAKQPIKFTKAYFNTGLNQKEQTIVTSTGPFAITWSLKKVLKGEKMSYRIKRYNSMVMEDNFRYGSDKNVILALKDNVKMAKKTGFKEPSKEMTRQNNWNEFLELSEKAAKRRNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DD55</accession>
    <name>C5DD55_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0B08426p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0B08426g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552154.1">
        <property type="nucleotide sequence ID" value="XM_002552108.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DD55"/>
    <dbReference type="FunCoup" id="C5DD55">
        <property type="interactions" value="358"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DD55"/>
    <dbReference type="GeneID" id="8290994"/>
    <dbReference type="KEGG" id="lth:KLTH0B08426g"/>
    <dbReference type="eggNOG" id="KOG3409">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067135_0_0_1"/>
    <dbReference type="InParanoid" id="C5DD55"/>
    <dbReference type="OMA" id="LMYPIDW"/>
    <dbReference type="OrthoDB" id="440760at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05791">
        <property type="entry name" value="S1_CSL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000312">
        <property type="entry name" value="Exosome complex component CSL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039771">
        <property type="entry name" value="Csl4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048626">
        <property type="entry name" value="CSL4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019495">
        <property type="entry name" value="EXOSC1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12686">
        <property type="entry name" value="3'-5' EXORIBONUCLEASE CSL4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12686:SF8">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT CSL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21551">
        <property type="entry name" value="CSL4_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10447">
        <property type="entry name" value="EXOSC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0271">Exosome</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exosome complex component CSL4 N-terminal" evidence="6">
        <location>
            <begin position="12"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="Exosome complex component CSL4 C-terminal" evidence="5">
        <location>
            <begin position="121"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="75"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="81"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF10447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF21551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR21716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="281" mass="30009" checksum="082E32DB02BA4F00" modified="2009-07-28" version="1">MVYYAQSTHSAYPGQLICPVYELEKGTDSQQVIRYIAGAGVAEQDYELNGQKIIALAATVVGEVKFDEELKEMVPSGEVAESNENEEEETVSTEEERHFIVSVTDKRQDGQAVTADFANNLPREGDIVLARVTRISQQRANVEILAVENKTTPVDSGVGSNGAGIVAAGGGSGGATFSVSQASSDLGETFRGLISSRDVRATDRDKVKIIDSFRPGDIVRAQVISLGDGSNYHLSTARNDLGVVFAKACNGAGGMMYAIDWKTMVAPSTGFTEARKCAKPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DD60</accession>
    <name>C5DD60_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Copper transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0B08536g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the copper transporter (Ctr) (TC 1.A.56) family. SLC31A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552159.1">
        <property type="nucleotide sequence ID" value="XM_002552113.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DD60"/>
    <dbReference type="FunCoup" id="C5DD60">
        <property type="interactions" value="567"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DD60"/>
    <dbReference type="GeneID" id="8290999"/>
    <dbReference type="KEGG" id="lth:KLTH0B08536g"/>
    <dbReference type="eggNOG" id="KOG3386">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079690_4_0_1"/>
    <dbReference type="InParanoid" id="C5DD60"/>
    <dbReference type="OMA" id="DITEYCH"/>
    <dbReference type="OrthoDB" id="161814at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005375">
        <property type="term" value="F:copper ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007274">
        <property type="entry name" value="Cop_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483:SF115">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 31 COPPER TRANSPORTERS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04145">
        <property type="entry name" value="Ctr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0186">Copper</keyword>
    <keyword evidence="4" id="KW-0187">Copper transport</keyword>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="47"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="103"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR21721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="150" mass="16857" checksum="78B6D921256199FB" modified="2009-07-28" version="1">MEHSMMDHGDMGHGDMGHDDMCSMNMLFTWNYKNTCVVFKWWHIRTLPHLLLSMIVVAASAYLYEYMKYYSAKSTASRAAGAANLSHTKAAKMKSASWYGAQVGFSFMLMLVFMTFNGWLMLAVVAGAAWGHYSWGHLTEGLAHNSLACH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>C5DD85</accession>
    <name>C5DD85_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glutamyl-tRNA(Gln) amidotransferase subunit A, mitochondrial</fullName>
            <shortName evidence="7">Glu-AdT subunit A</shortName>
            <ecNumber evidence="7">6.3.5.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">HER2</name>
        <name evidence="9" type="ordered locus">KLTH0B09152g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>L-glutamyl-tRNA(Gln) + L-glutamine + ATP + H2O = L-glutaminyl-tRNA(Gln) + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.7"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Subunit of the heterotrimeric GatFAB amidotransferase (AdT) complex, composed of A, B and F subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the amidase family. GatA subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.7" evidence="7"/>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552184.1">
        <property type="nucleotide sequence ID" value="XM_002552138.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DD85"/>
    <dbReference type="FunCoup" id="C5DD85">
        <property type="interactions" value="369"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DD85"/>
    <dbReference type="GeneID" id="8291027"/>
    <dbReference type="KEGG" id="lth:KLTH0B09152g"/>
    <dbReference type="eggNOG" id="KOG1211">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009600_7_6_1"/>
    <dbReference type="InParanoid" id="C5DD85"/>
    <dbReference type="OMA" id="QPASYCG"/>
    <dbReference type="OrthoDB" id="421993at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030956">
        <property type="term" value="C:glutamyl-tRNA(Gln) amidotransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070681">
        <property type="term" value="P:glutaminyl-tRNAGln biosynthesis via transamidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00120">
        <property type="entry name" value="GatA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000120">
        <property type="entry name" value="Amidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020556">
        <property type="entry name" value="Amidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004412">
        <property type="entry name" value="GatA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00132">
        <property type="entry name" value="gatA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895:SF7">
        <property type="entry name" value="GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE SUBUNIT A, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895">
        <property type="entry name" value="TRANSAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00571">
        <property type="entry name" value="AMIDASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="8">
        <location>
            <begin position="15"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="7">
        <location>
            <position position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR21746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="465" mass="50391" checksum="6B43B7B447111384" modified="2009-07-28" version="1">MSFKSAISRLEKLPLIQKQYNAFTHLNPNCKELLIKDVSSSPKKSLTNLLYGIKDNIATSDMPTSCGSKVLHNYQSPFDATIVELLESAGAISVGKTNLDEFGMGSGGLFSHFGPTKNPLFPFQDTVVGGSSSGSAAAVAADAVDFSIGTDTGGSVRLPAAYTSLFGFKPSYGRISRFGVIAYAQSMDTVGIFSKNVSIIKSVFDVLDKHDKKDPTSLSENLRGKLRQLSSPQSSYKIGIPQELLQDNMPLELQDGFLLALEKLEANGHEIYGVSIPQMVNSLPIYYTLAPAEAASNLARFDGLRYGYRDDVADSRDDTLFAPTRSAFGDEVKSRIVLGNYNLCSESFNNHYIKAQRLRVDLINEFDNTFTHPNILTGFPGNQNGVDFLISFSSINLPPAMSDYDSDCTSNPTNSYVNDVFTTPMSLAGLPAINVPVMASNPIGIQLVGQYGDDSRLLDLAAELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDT1</accession>
    <name>C5DDT1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0C03564p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0C03564g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR21942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552380.1">
        <property type="nucleotide sequence ID" value="XM_002552334.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDT1"/>
    <dbReference type="FunCoup" id="C5DDT1">
        <property type="interactions" value="203"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDT1"/>
    <dbReference type="GeneID" id="8291244"/>
    <dbReference type="KEGG" id="lth:KLTH0C03564g"/>
    <dbReference type="eggNOG" id="ENOG502QRI1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_492622_0_0_1"/>
    <dbReference type="InParanoid" id="C5DDT1"/>
    <dbReference type="OMA" id="DCLLFHT"/>
    <dbReference type="OrthoDB" id="4058916at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032054">
        <property type="entry name" value="Cdt1_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16679">
        <property type="entry name" value="CDT1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA replication factor Cdt1 C-terminal" evidence="1">
        <location>
            <begin position="444"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR21942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="567" mass="64138" checksum="F0160C66DCBBD584" modified="2009-07-28" version="1">MLKVVDLNVITTEDELLPIMKSVLRNESVILLKNYANMETLRTLSGILAKDSPDDLAGFDSNFVGCTKLENGVSVEQFIGSADSVGVQNTALSKMSSRLVKIAIFFARICLAAVNFEKEEAVLNEHNSAVKVTRYHKPISKSVLDLEFDYEDEYVLHNSPGIITVFPVAHGVQFKEHGKWAPLQDPDSILIQTGTLLSHFSNGMHASDSVKISTTNTTYLTIFPALESFVNGQTVSRLLLLNQLQEFPQVAEVLYPKEMAQQNLVKRVDFCKGIFNVTDSVMSLYCVSRSLTSSNPELDKLLPQISNMLKKKVSQDDFLRMMYIWDEAYILEVGNDHEITIGLPPSGLLNTLTNKSRKLRYTELADKWLSDAILQPQILANVPALKIRKRRASSTSDIQGGQVQELHAPRALAQPKKNRTRGYIANTKEKFMFKELSNNENGESLLERIREKERRAAALLSQRERQYEQFLNVKTFQVFDILNSLTPDRPYTTTHLISLIVDSLVDSNNPIGERETYDILLRLQALLKDKIKIIEAEGSLKVFRWPSLDRLVLEKRIKESNEQLFNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DDZ7</accession>
    <name>C5DDZ7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">UDP-N-acetylglucosamine transferase subunit ALG14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 11">Asparagine-linked glycosylation protein 14</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ALG14</name>
        <name evidence="12" type="ordered locus">KLTH0C05082g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Involved in protein N-glycosylation. Essential for the second step of the dolichol-linked oligosaccharide pathway. Anchors the catalytic subunit ALG13 to the ER.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 11">Heterodimer with ALG13 to form a functional enzyme.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Endoplasmic reticulum membrane</location>
            <topology evidence="1 11">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the ALG14 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552446.1">
        <property type="nucleotide sequence ID" value="XM_002552400.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DDZ7"/>
    <dbReference type="FunCoup" id="C5DDZ7">
        <property type="interactions" value="322"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DDZ7"/>
    <dbReference type="GeneID" id="8291313"/>
    <dbReference type="KEGG" id="lth:KLTH0C05082g"/>
    <dbReference type="eggNOG" id="KOG3339">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064541_2_2_1"/>
    <dbReference type="InParanoid" id="C5DDZ7"/>
    <dbReference type="OMA" id="GTCCIIT"/>
    <dbReference type="OrthoDB" id="17098at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043541">
        <property type="term" value="C:UDP-N-acetylglucosamine transferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004577">
        <property type="term" value="F:N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013969">
        <property type="entry name" value="Oligosacch_biosynth_Alg14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12154">
        <property type="entry name" value="GLYCOSYL TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12154:SF4">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE TRANSFERASE SUBUNIT ALG14 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08660">
        <property type="entry name" value="Alg14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR22008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="228" mass="25774" checksum="DDBA790A4F108C5B" modified="2009-07-28" version="1">MGYTLCLFALVFLQALVVRTLWVIPIFGIREGAELSEKRGLQAGRALRLFVFLGSGGHTGEMLRLLDNYRTALLRPGTQLYVGFSDEASLRKFQTTVGANLSGVEVQFLRFSKAREVGASWLASTRSVAFSFVQSLWCLTWLKWQFVGQQHLVLLNGPGTCCIIASWLKMLEIVTLTRSKIVYVESLARTDTLSLSGKLLYPLVDEFVVQWDELYERYDRARCYGILV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>C5DE03</accession>
    <name>C5DE03_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase</fullName>
            <ecNumber evidence="7">2.1.1.205</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">2'-O-ribose RNA methyltransferase TRM7 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">TRM7</name>
        <name evidence="9" type="ordered locus">KLTH0C05214g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Methylates the 2'-O-ribose of nucleotides at positions 32 and 34 of the tRNA anticodon loop of substrate tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>cytidine(32)/guanosine(34) in tRNA + 2 S-adenosyl-L-methionine = 2'-O-methylcytidine(32)/2'-O-methylguanosine(34) in tRNA + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:42396"/>
            <dbReference type="Rhea" id="RHEA-COMP:10246"/>
            <dbReference type="Rhea" id="RHEA-COMP:10247"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74445"/>
            <dbReference type="ChEBI" id="CHEBI:74495"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.205"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family. TRM7 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.205" evidence="7"/>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552452.1">
        <property type="nucleotide sequence ID" value="XM_002552406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DE03"/>
    <dbReference type="FunCoup" id="C5DE03">
        <property type="interactions" value="963"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DE03"/>
    <dbReference type="GeneID" id="8291319"/>
    <dbReference type="KEGG" id="lth:KLTH0C05214g"/>
    <dbReference type="eggNOG" id="KOG1099">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009422_1_2_1"/>
    <dbReference type="InParanoid" id="C5DE03"/>
    <dbReference type="OMA" id="FIVCLNF"/>
    <dbReference type="OrthoDB" id="289250at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106340">
        <property type="term" value="F:tRNA (guanosine(34)-2'-O)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002128">
        <property type="term" value="P:tRNA nucleoside ribose methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000176">
        <property type="entry name" value="tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01547">
        <property type="entry name" value="RNA_methyltr_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03162">
        <property type="entry name" value="RNA_methyltr_E_TRM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028590">
        <property type="entry name" value="RNA_methyltr_E_TRM7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050082">
        <property type="entry name" value="RNA_methyltr_RlmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002877">
        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015507">
        <property type="entry name" value="rRNA-MeTfrase_E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920">
        <property type="entry name" value="RIBOSOMAL RNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920:SF12">
        <property type="entry name" value="TRNA (CYTIDINE(32)_GUANOSINE(34)-2'-O)-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01728">
        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 7" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Ribosomal RNA methyltransferase FtsJ" evidence="8">
        <location>
            <begin position="21"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="161"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR22014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="303" mass="34348" checksum="5F63428BFA9DBF29" modified="2009-07-28" version="1">MGKSSKDKRDLYYRKAKEEGYRARSAFKLLQLDEQFHFLDNVTRVVDLCAAPGSWSQVLSRRLFDDDTENNSSKRIVAVDLQPMFPIDNVTTIQADITHPKTLNKIMDLFEGEKADFVCSDGAPDVTGLHDLDEYVQQQLILSALQLTTCLLREGGVFVAKIFRGRDIDMLYSQLSFLFERVVCAKPRSSRGTSLESFIVCLGYRPPSDWKPRLDVNMSVEDFFQGCDIGRLTIDDEIPNYREEPRSIAEFISCGGLDSFDSDATYHDVPENTTALDPVQPPTNPPYKRALELKRQGKLTRAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DE20</accession>
    <name>C5DE20_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">AP-1 complex subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0C05610g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the adaptor complexes large subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552469.1">
        <property type="nucleotide sequence ID" value="XM_002552423.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DE20"/>
    <dbReference type="FunCoup" id="C5DE20">
        <property type="interactions" value="694"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DE20"/>
    <dbReference type="GeneID" id="8291336"/>
    <dbReference type="KEGG" id="lth:KLTH0C05610g"/>
    <dbReference type="eggNOG" id="KOG1062">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003824_0_1_1"/>
    <dbReference type="InParanoid" id="C5DE20"/>
    <dbReference type="OMA" id="AICAMRI"/>
    <dbReference type="OrthoDB" id="28053at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030121">
        <property type="term" value="C:AP-1 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050840">
        <property type="entry name" value="Adaptor_Complx_Large_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017107">
        <property type="entry name" value="AP1_complex_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008152">
        <property type="entry name" value="Clathrin_a/b/g-adaptin_app_Ig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013041">
        <property type="entry name" value="Clathrin_app_Ig-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22780">
        <property type="entry name" value="ADAPTIN, ALPHA/GAMMA/EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02883">
        <property type="entry name" value="Alpha_adaptinC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037094">
        <property type="entry name" value="AP1_complex_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00809">
        <property type="entry name" value="Alpha_adaptinC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49348">
        <property type="entry name" value="Clathrin adaptor appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5 8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Clathrin adaptor alpha/beta/gamma-adaptin appendage Ig-like subdomain" evidence="9">
        <location>
            <begin position="689"/>
            <end position="796"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR037094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR22031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="797" mass="89148" checksum="D893E5C37E5D9E27" modified="2009-07-28" version="1">MGSLRSFIKDVRAAKTLAEERSIVTKESARIRTKLKDDHLPQEKRRKNIHKLLYLHILGEKTHFAQVECINLIASEDFRDKRLGYLAAMILLDENQEILTLLTNMLNNDLNHPNRYVVSLALSTLGSLMSPELARDLYSDVENILAHSKDDFLVKKALQCAAKLIQKDTSLVEVFFPYINSRLVSNQQSSHGVLLGVAKLCQAAITAKEKYEYDNYPEILQSMVQSIPEFFALLQDMNFTSFNPEYDVGGTCDPFLQVELLYTIRLLFELAPQETESYKDKLNDLLTKIATNSDGAKNSAHAVLYECVRTIFALQLDQSLKVLGVNVLAKFLSGKDNNTKYVALNTLLHVVPQEPQAVQKHRKFISKCLFDPDISIKTRAVELTFAILNDSNIKELIEELTAFLKLTSEDDKDLATYVVEHMIGLFEQYKLGDEKWALEATVNILMIVGEHISLERLSDILIMINNARDLDHKIQVIRRILDVSFAKSGESVSSANLGWKLVSAWCIGEYGDTLLESNIYLDTELTGYLRTLDGLYSDDCKIIGYVLTAALKLSSRLQNPSCIEELRQVIKGHNKDTNLMIQTKSVQYGILFNLPAKEKHDILGAMPLFEKKPKSGAAPAARPKFGTKPQEPDLLLELLDDDYPKKSTTSNAAGSLLDIFGSAPQSSAKPENGKGNDTAGLTQNLMTIPKDAPEAFRSTDLDVHMKLKSLNDYEAHVEMFIHANASINSLQVLAAVSKSKKLTLGSLSASTLAPRENCRQEMIITGGGKPKLRIKLTFNTGSPSITKTEQFDYKFDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>C5DE29</accession>
    <name>C5DE29_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0C05830p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0C05830g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552478.1">
        <property type="nucleotide sequence ID" value="XM_002552432.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DE29"/>
    <dbReference type="FunCoup" id="C5DE29">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DE29"/>
    <dbReference type="GeneID" id="8291346"/>
    <dbReference type="KEGG" id="lth:KLTH0C05830g"/>
    <dbReference type="eggNOG" id="KOG4781">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052827_2_2_1"/>
    <dbReference type="InParanoid" id="C5DE29"/>
    <dbReference type="OMA" id="GRKCIIT"/>
    <dbReference type="OrthoDB" id="506431at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="CDD" id="cd03443">
        <property type="entry name" value="PaaI_thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052061">
        <property type="entry name" value="PTE-AB_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47260:SF4">
        <property type="entry name" value="MIOREX COMPLEX COMPONENT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47260">
        <property type="entry name" value="UPF0644 PROTEIN PB2B4.06"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioesterase" evidence="1">
        <location>
            <begin position="137"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="246" mass="27645" checksum="9EEAE754E97EADB8" modified="2009-07-28" version="1">MSRPFLARYIAVPAAGFALGFATFLRAWPTPHAGDDGPDNETLQSATLKRPAEQQRLDILEHLQQHRIYADLIRRDNIQQWSQSERITRPHRDYHVGQGLLYGPGSLEIDPLVLHDPDAQELVVFYHLGRNLANEKGAVHKGVISLLLDEALCYCGFPSLPSKSGVTARLDLQFHQDVPTDCTVVLKARVQEARGRKCIITGSLHTLPSRPWYRPFGGESSKTLATATCVLVEPKWFKYVKWLNAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5DE75</accession>
    <name>C5DE75_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0C06886p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0C06886g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="5">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552524.1">
        <property type="nucleotide sequence ID" value="XM_002552478.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DE75"/>
    <dbReference type="FunCoup" id="C5DE75">
        <property type="interactions" value="1157"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DE75"/>
    <dbReference type="MEROPS" id="T01.P02"/>
    <dbReference type="GeneID" id="8291393"/>
    <dbReference type="KEGG" id="lth:KLTH0C06886g"/>
    <dbReference type="eggNOG" id="KOG0180">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035750_10_0_1"/>
    <dbReference type="InParanoid" id="C5DE75"/>
    <dbReference type="OMA" id="CSEQLYG"/>
    <dbReference type="OrthoDB" id="204949at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010499">
        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03759">
        <property type="entry name" value="proteasome_beta_type_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000003">
        <property type="entry name" value="Proteasome subunit beta type-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033811">
        <property type="entry name" value="Proteasome_beta_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF10">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA TYPE-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0647">Proteasome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00026071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR22086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="205" mass="22615" checksum="F03B88C2DADE56CB" modified="2009-07-28" version="1">MSDPSSINGGIVVAMTGKDCVAIACDLRLGSQSLGVANNFEKIFHYGHVFLGLTGLATDVTTLSETFRYKTNMYKLKEDRFIEPETFTQLVSSTLYEKRFGPYFTGPVVAGINSRTGKPYISGFDLIGCIDEAKDFVVSGTASEQLFGMCESLYEPNLEPEDLFETISQALLNAADRDALSGWGAAVYIIKKDKVIKRYLKTRQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEB8</accession>
    <name>C5DEB8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Serine--tRNA ligase, cytoplasmic</fullName>
            <ecNumber evidence="3">6.1.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Seryl-tRNA synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Seryl-tRNA(Ser) synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">KLTH0C07898g</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the attachment of serine to tRNA(Ser) in a two-step reaction: serine is first activated by ATP to form Ser-AMP and then transferred to the acceptor end of tRNA(Ser).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.11" evidence="3"/>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552567.1">
        <property type="nucleotide sequence ID" value="XM_002552521.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEB8"/>
    <dbReference type="FunCoup" id="C5DEB8">
        <property type="interactions" value="869"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEB8"/>
    <dbReference type="GeneID" id="8291439"/>
    <dbReference type="KEGG" id="lth:KLTH0C07898g"/>
    <dbReference type="eggNOG" id="KOG2509">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023797_0_1_1"/>
    <dbReference type="InParanoid" id="C5DEB8"/>
    <dbReference type="OMA" id="GYTPCFR"/>
    <dbReference type="OrthoDB" id="10264585at2759"/>
    <dbReference type="UniPathway" id="UPA00906">
        <property type="reaction ID" value="UER00895"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004828">
        <property type="term" value="F:serine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006434">
        <property type="term" value="P:seryl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00770">
        <property type="entry name" value="SerRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.40:FF:000003">
        <property type="entry name" value="Serine--tRNA ligase cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000026">
        <property type="entry name" value="Seryl-tRNA synthetase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.40">
        <property type="entry name" value="Serine-tRNA synthetase, tRNA binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002317">
        <property type="entry name" value="Ser-tRNA-ligase_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015866">
        <property type="entry name" value="Ser-tRNA-synth_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042103">
        <property type="entry name" value="SerRS_1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033729">
        <property type="entry name" value="SerRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010978">
        <property type="entry name" value="tRNA-bd_arm"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00414">
        <property type="entry name" value="serS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11778">
        <property type="entry name" value="SERYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02403">
        <property type="entry name" value="Seryl_tRNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001529">
        <property type="entry name" value="Ser-tRNA-synth_IIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00981">
        <property type="entry name" value="TRNASYNTHSER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46589">
        <property type="entry name" value="tRNA-binding arm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="16">
        <location>
            <begin position="198"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="76"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="279"/>
            <end position="281"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="279"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="295"/>
            <end position="298"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="366"/>
            <end position="369"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="402"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for serine binding" evidence="13">
        <location>
            <position position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00058708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00074532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR001529-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR001529-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAR22129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="461" mass="53013" checksum="BB07EFE48E477000" modified="2009-07-28" version="1">MLDITQFIEEKGGNPELIRQSQKARHASVEIVDEIIADYKEWVKTRFQLDELNKQQNKVQKEIGLKFKNKEDPKELLAEKDRIAAEKKKLSELEQKQDNDLKQKVFQVGNIVHPSVVVSNDEENNELVRSWKPENLSEVGTNASCSGKPASLSHHEVLLRLDGYDPERGVKISGHRGYFFRNYGVFLNQAMINYGLSFLASKGYIPLQAPVMMDKEAMSKTAQLSEFDEELYKVMDGDDEKYLIATSEQPISAYHSGEWFEKPQEQLPVRYAGYSSCFRREAGAHGKDAWGVFRVHAFEKIEQFVLTEPEKSWEEFDRMIATSEEFYQSLNLPYRVVGIVSGELNNAAAKKYDLEAWFPYQQEYKELVSCSNCTDYQSRNLEIRCGIKKMGDREKKYVHCLNSTLCATQRALCCVLENYQTEEGLVVPEVLRKYIPGEPEFIPFTKELPKNSTSNKKKGNN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-03-08" modified="2025-06-18" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEI4</accession>
    <name>MZM1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName>Mitochondrial zinc maintenance protein 1, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">MZM1</name>
        <name type="ordered locus">KLTH0C09482g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Assembly factor required for Rieske Fe-S protein RIP1 incorporation into the cytochrome b-c1 (CIII) complex. Functions as a chaperone, binding to this subunit within the mitochondrial matrix and stabilizing it prior to its translocation and insertion into the late CIII dimeric intermediate within the mitochondrial inner membrane. Modulates the mitochondrial matrix zinc pool.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with RIP1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complex I LYR family. MZM1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552633.1">
        <property type="nucleotide sequence ID" value="XM_002552587.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEI4"/>
    <dbReference type="SMR" id="C5DEI4"/>
    <dbReference type="FunCoup" id="C5DEI4">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DEI4"/>
    <dbReference type="GeneID" id="8291511"/>
    <dbReference type="KEGG" id="lth:KLTH0C09482g"/>
    <dbReference type="eggNOG" id="ENOG502S6EF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_147114_2_2_1"/>
    <dbReference type="InParanoid" id="C5DEI4"/>
    <dbReference type="OMA" id="KYKLRIH"/>
    <dbReference type="OrthoDB" id="529194at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034551">
        <property type="term" value="P:mitochondrial respiratory chain complex III assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20267">
        <property type="entry name" value="Complex1_LYR_LYRM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045298">
        <property type="entry name" value="Complex1_LYR_LYRM7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050435">
        <property type="entry name" value="MZM1/LYRM7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46749">
        <property type="entry name" value="COMPLEX III ASSEMBLY FACTOR LYRM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46749:SF1">
        <property type="entry name" value="COMPLEX III ASSEMBLY FACTOR LYRM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0496">Mitochondrion</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0809">Transit peptide</keyword>
    <feature type="transit peptide" description="Mitochondrion" evidence="2">
        <location>
            <begin position="1"/>
            <end status="unknown"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000405496" description="Mitochondrial zinc maintenance protein 1, mitochondrial">
        <location>
            <begin status="unknown"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="118" mass="12952" checksum="2F95BCC1E6BCF940" modified="2009-07-28" version="1" precursor="true">MSTSSMALAAYRNGLRATKVAFGQDLRMLQAARSKMREGMANPPNPELAPEQQIQHLNEVSQFLRRNIVQGKRGEGDKYTLNIHKETELGDNETIKTTKKTLVSRGGGCCGGGQGLYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5DER0</accession>
    <name>C5DER0_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">U three protein 23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0C11352g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in rRNA-processing and ribosome biogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the UTP23/FCF1 family. UTP23 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928167">
        <property type="protein sequence ID" value="CAR22271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552709.1">
        <property type="nucleotide sequence ID" value="XM_002552663.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DER0"/>
    <dbReference type="FunCoup" id="C5DER0">
        <property type="interactions" value="1125"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DER0"/>
    <dbReference type="GeneID" id="8291595"/>
    <dbReference type="KEGG" id="lth:KLTH0C11352g"/>
    <dbReference type="eggNOG" id="KOG3164">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053567_1_1_1"/>
    <dbReference type="InParanoid" id="C5DER0"/>
    <dbReference type="OMA" id="CCMQALY"/>
    <dbReference type="OrthoDB" id="25675at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome C"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09865">
        <property type="entry name" value="PIN_ScUtp23p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000006">
        <property type="entry name" value="rRNA-processing protein UTP23 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006984">
        <property type="entry name" value="Fcf1/UTP23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057776">
        <property type="entry name" value="UTP23_sensor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12416">
        <property type="entry name" value="RRNA-PROCESSING PROTEIN UTP23 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04900">
        <property type="entry name" value="Fcf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24779">
        <property type="entry name" value="UTP23_sensor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="UTP23 sensor motif region" evidence="9">
        <location>
            <begin position="198"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="169"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="215"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="242"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00076388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF24779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR22271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="268" mass="30288" checksum="6760CC02ADC5205F" modified="2009-07-28" version="1">MRQKRAKSYRKQMLVYNHNFKFREPYQVLVDDQIVCDTHRASFDLVKGLQRTLQAEVKPMITQCCIQAIYETKNQDAIELAKSFERRRCNHPPKEAKPPIECLQSVVSVNGNNKHRYVVASQDPSIRKKLRQVPGVPLIYMNRSVMVMEPLSKASGDFSRAQEKEKLFKGLNDPKYAGLPAEEEGGKTDNGTEVSKNKRKGPKGPNPLSIKKKKTVPDSKDESPPSDKISVTDAEPVDRSSSGRRRRRHHKRSSTEAGEEGESGNISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>C5DEW8</accession>
    <name>C5DEW8_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0D10384p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0D10384g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553161.1">
        <property type="nucleotide sequence ID" value="XM_002553115.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DEW8"/>
    <dbReference type="FunCoup" id="C5DEW8">
        <property type="interactions" value="122"/>
    </dbReference>
    <dbReference type="GeneID" id="8295400"/>
    <dbReference type="KEGG" id="lth:KLTH0D10384g"/>
    <dbReference type="eggNOG" id="ENOG502S1FA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085163_0_0_1"/>
    <dbReference type="InParanoid" id="C5DEW8"/>
    <dbReference type="OMA" id="YYTPLAF"/>
    <dbReference type="OrthoDB" id="4069757at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="160"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="195"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="214" mass="23644" checksum="E3EB55D393B45CA5" modified="2009-07-28" version="1">MLHTTPKTQILITDVPAQDFACGWPQQLQSQLFDREFPQLKHSCLYYTPLAFLHRVVLIFDQEPATLQVHEFLTRALAGKPVKIFLTESLLAKPRAKSFDAAPDHVPDFDVEPRAKPQLSLDTHSSPANSPSLSPDRSDPHSPTALKSLEDGKVHLYQEPLPAADAPGDDASPRGETKLLYKPRLSLDTRSSGASHSSFSVPMSPSITLNEFPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>C5DF18</accession>
    <name>C5DF18_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0D11528p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0D11528g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553211.1">
        <property type="nucleotide sequence ID" value="XM_002553165.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DF18"/>
    <dbReference type="GeneID" id="8295451"/>
    <dbReference type="KEGG" id="lth:KLTH0D11528g"/>
    <dbReference type="eggNOG" id="ENOG502T0NQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1409005_0_0_1"/>
    <dbReference type="InParanoid" id="C5DF18"/>
    <dbReference type="OMA" id="CSFTNAF"/>
    <dbReference type="OrthoDB" id="4036615at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002948704" evidence="1">
        <location>
            <begin position="20"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="193" mass="19462" checksum="460DB0FDEBEAC568" modified="2009-07-28" version="1">MRFTTVVASLIAASGFASASEYLNQTTTLRPSGSSSLLNAVTTELTTQTFDSTSTVTLMTTITMSGGQSTGSTTTSAASASSAALTAGQNNKGAFVGSASSASSSTSEIWSTITSTLYTTQSVTLSNSQVSASVSAYEKQIVIGTSIPSCTPQTVTVTAEKTKYITVTAGQTSSAAPVYTPSAGPYSNSTMSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DF57</accession>
    <name>C5DF57_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0D12408p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0D12408g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the palA/RIM20 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553250.1">
        <property type="nucleotide sequence ID" value="XM_002553204.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DF57"/>
    <dbReference type="FunCoup" id="C5DF57">
        <property type="interactions" value="1239"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DF57"/>
    <dbReference type="GeneID" id="8295490"/>
    <dbReference type="KEGG" id="lth:KLTH0D12408g"/>
    <dbReference type="eggNOG" id="KOG2220">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007181_3_1_1"/>
    <dbReference type="InParanoid" id="C5DF57"/>
    <dbReference type="OMA" id="VSHAEEM"/>
    <dbReference type="OrthoDB" id="64867at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09241">
        <property type="entry name" value="BRO1_ScRim20-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08915">
        <property type="entry name" value="V_Alix_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.560">
        <property type="entry name" value="alix/aip1 in complex with the ypdl late domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.50">
        <property type="entry name" value="alix/aip1 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.280">
        <property type="entry name" value="alix/aip1 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025304">
        <property type="entry name" value="ALIX_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004328">
        <property type="entry name" value="BRO1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038499">
        <property type="entry name" value="BRO1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23030">
        <property type="entry name" value="PCD6 INTERACTING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23030:SF39">
        <property type="entry name" value="PROGRAMMED CELL DEATH 6-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13949">
        <property type="entry name" value="ALIX_LYPXL_bnd"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03097">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01041">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51180">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRO1" evidence="2">
        <location>
            <begin position="3"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR22812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="660" mass="75021" checksum="7FAC5798350BF10C" modified="2009-07-28" version="1">MSQLLCIPLKRSLKVDLKSGLSNLINDNFYQVASVFGNDLETIDKMRDAALEADISPEGVEALHNYYLQLIQLEKKFPDNQLSFKWFETLSLKSYGKEDSRFVFEKLNIVYDIGALYSLLAADSNNGTTEGLKKACIFSQKSAGCFSFIAKELKEMSDPIMEKKAIQCLEQLMLAQAQELFWLKAIKDGLKNSIIARLALQVSLYYKASYKQAASSELIRSDWDKRLKDKASYFEAVTSFRYSLIFDEKGDYGSKVKSLRDASEHLRHVSAQDDTAELFRRKVSEALKTAERDNDLIYLQNIPTTIPPLKPASMVKSINFEDISGTPSSTGSAACAPLFKELLPNFVMESSNAFSQRQEQYFQQYIVAPLNAMNKMLLEKLPSYEIPGALHPIDPEELDNCERSMNDLKLNSQHVGDILGQVFSLLSQESDTDKLLRSKFGTLSWTIAPSEEVNQDYWSKYHKIKKYLQEGESVDYETTQQFEVIDKKLLTAPIKLPESNNPLVKEVSATLKRREDLILKTENKSSRSTILPKIISAYKKTGQTEFEGLFAEHLKMFDKEISAVEGEKNLNNKLIEKLEAQVEGDRQMQRIDARDIFVQDFKHSLKLLGDVKENIIGGSKFYNDLITSAGSLLTDVQQFEAQRKEQKRNLEDALLKSSYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFD2</accession>
    <name>C5DFD2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0D14168p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0D14168g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553325.1">
        <property type="nucleotide sequence ID" value="XM_002553279.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFD2"/>
    <dbReference type="FunCoup" id="C5DFD2">
        <property type="interactions" value="78"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFD2"/>
    <dbReference type="GeneID" id="8295569"/>
    <dbReference type="KEGG" id="lth:KLTH0D14168g"/>
    <dbReference type="eggNOG" id="ENOG502S4GP">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_157410_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFD2"/>
    <dbReference type="OMA" id="TLANHYY"/>
    <dbReference type="OrthoDB" id="4067990at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020195">
        <property type="entry name" value="SWR1_Swc7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17330">
        <property type="entry name" value="SWC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005668088" evidence="1">
        <location>
            <begin position="18"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR22887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="129" mass="15069" checksum="393960FB745A62A9" modified="2009-07-28" version="1">MGLKSNVALLLLQIVLYRQQELLHMDKTLDNDQLLADPVLDEMILSKFANHRLVRMYAPELSGIQLRTLKGIVRELFEQGLPDDDVPVTVVTLANHFYYTRVQELEREQIPELKAKVQELQNEREQERT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFD3</accession>
    <name>C5DFD3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0D14190p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0D14190g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VAC14 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553326.1">
        <property type="nucleotide sequence ID" value="XM_002553280.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFD3"/>
    <dbReference type="FunCoup" id="C5DFD3">
        <property type="interactions" value="945"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFD3"/>
    <dbReference type="GeneID" id="8295570"/>
    <dbReference type="KEGG" id="lth:KLTH0D14190g"/>
    <dbReference type="eggNOG" id="KOG0212">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007740_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFD3"/>
    <dbReference type="OMA" id="QCYQHVS"/>
    <dbReference type="OrthoDB" id="5574975at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070772">
        <property type="term" value="C:PAS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006661">
        <property type="term" value="P:phosphatidylinositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000739">
        <property type="entry name" value="VAC14p Enzyme regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026825">
        <property type="entry name" value="Vac14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021841">
        <property type="entry name" value="VAC14_Fig4p-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16023:SF0">
        <property type="entry name" value="PROTEIN VAC14 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16023">
        <property type="entry name" value="TAX1 BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12755">
        <property type="entry name" value="Vac14_Fab1_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11916">
        <property type="entry name" value="Vac14_Fig4_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="HEAT" evidence="5">
        <location>
            <begin position="89"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="Vacuolar protein 14 C-terminal Fig4-binding" evidence="7">
        <location>
            <begin position="578"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="335"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="838"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="335"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="355"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="373"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="846"/>
            <end position="858"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF11916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR22888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="865" mass="97413" checksum="A24E7FC1F8E09545" modified="2009-07-28" version="1">MSKLKVERKGLNDKLYEKRKATALNLEKLVKQCLAEGDFQRVDKIFDELCRDFAYALHQPMARNAGLMGLAAGAIALGSTEVARYLDRILPPVLACFGDQNDQVRFYACESLYNIAKIAKGEILVYFNEIFDVLCKISADTETSIRGAAELLDRLIKDIVAERASNYVSVVNNDPKDLPLATRTDPQTGEVVQEEYNQDPEFAFSLPKFIPLLTERIYAINPDTRMFLVSWLQVLENIPDLELISYLPSFLGGLFTFLGDSHKDVRTVTHSLLDLLLHEVQRISEVKALVQKKLLEEAPKRLVSEDNASKKQDGILISERKKSLLTAFEQLSMTDAANPQKNRNSSNVDELKSNDGGNTINEISETNSANKSKSAELSDSRNGDLYCPGQDIHLDFPKIIEILINTLGLSEPEIQLVALTWIETVLGISATSFLPFVSRLLSLLLKILNDVDPKVRKLAHSVNERLIELTGRFDDREGPEAINYGPVVNTLTLHFLDSDVVAKVACLEWLILIYHKVPDELLEHSDSTFLTLLKSLSDKDNQLTSKALQLLSDLCNNSNEEYFKKFIRDLLLLFKNDRKLLKTRANHIFRQLCVKLTAEKVYNVVASILEHDGNTAFVRMMIQILNTNLITAPELGHLRRKLRASSGEELFSSLFKCWSHNPVSLLALCLLSEKYELAYYILQTFVEYEISVNDLVQIDILIQLLESPIFTGLRLQLLEQDQYPYLYKCLYGILMVLPQSKAFQILNTRLNSVSKLAAQKIPSSKLYSLRNGSGIASGASLSSESLNSTSSGYRKNNMFQSSLDHFRNVCESELSTQMSTSAEESNFALLDEVLTPRTMPVSGRQQSEDSSDHYKDDSESVIFRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFH3</accession>
    <name>C5DFH3_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein LOT5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0D15092g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the LOT5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553366.1">
        <property type="nucleotide sequence ID" value="XM_002553320.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFH3"/>
    <dbReference type="FunCoup" id="C5DFH3">
        <property type="interactions" value="53"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFH3"/>
    <dbReference type="GeneID" id="8295610"/>
    <dbReference type="KEGG" id="lth:KLTH0D15092g"/>
    <dbReference type="eggNOG" id="ENOG502RY1I">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073622_0_0_1"/>
    <dbReference type="InParanoid" id="C5DFH3"/>
    <dbReference type="OMA" id="NSCIIIW"/>
    <dbReference type="OrthoDB" id="19714at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039924">
        <property type="entry name" value="ICln/Lot5/Saf5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03517">
        <property type="entry name" value="Voldacs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR22928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="304" mass="33771" checksum="F5FDB49D62E54913" modified="2009-07-28" version="1">MAGTAEAELICQLESTKPTVENVTPYSSYQQTQPRLKGFSLYQQNELPVLYGGGREFLLSLIPSGDEPADMVQAEVVDFFILNTCMVIWINTLDRGLRIPYQKVVYHGVHPIESPTASAEGHSLEIVLTVQRDATLNQLFPAGAPTRAADPLGEFTMSTVELILRPKYADFDRHYNDQMENLFTFKEFGLNRGDTLVVNCSKAIATCMDFFCVEESDDETETDQTASSALPGFTGVGEFLQEPPVYFNNGAADDLGEDDRSMSRFLVDGTDAGMSLEFCGNEQLAGRKKLWEHDGPEFPNKYRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DFM7</accession>
    <name>C5DFM7_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RBR-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">KLTH0D16368g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the RBR family. RNF14 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="3"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553420.1">
        <property type="nucleotide sequence ID" value="XM_002553374.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DFM7"/>
    <dbReference type="FunCoup" id="C5DFM7">
        <property type="interactions" value="186"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DFM7"/>
    <dbReference type="GeneID" id="8295667"/>
    <dbReference type="KEGG" id="lth:KLTH0D16368g"/>
    <dbReference type="eggNOG" id="KOG1814">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021364_2_2_1"/>
    <dbReference type="InParanoid" id="C5DFM7"/>
    <dbReference type="OMA" id="SNLQRCP"/>
    <dbReference type="OrthoDB" id="1431934at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20354">
        <property type="entry name" value="Rcat_RBR_RNF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23783">
        <property type="entry name" value="RWD_ScITT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047548">
        <property type="entry name" value="Rcat_RBR_RNF14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="13">
        <location>
            <begin position="179"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="12">
        <location>
            <begin position="183"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAR22982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="464" mass="53396" checksum="6B656102761A149F" modified="2009-07-28" version="1">MGEETTRADEDVEILKCMYPELEVAQESEHLIEARLAFTILSLTEVNVTWGSNDSLAPENREVRMPNFPGNEIRIVCQRKHYPDFKRGLHYSIKSEWMTEADIKRLDSEISQEFACQCESESDSFDTSFPLLMMLFDFLINNSSPVLFPLDEYKCETWKQFQVIKKLKDEISQLEFDSLKLDCCICLETKRGADMVKLPCNEHVLCKACVESYYSTMISEGRINNVRCPECPYSEVTPSDYNNFKELKAALMTPVIPFKFFEGLLSAGTCERYEKFFYEQAFAALYQFSPFSCIVCPRCESWAPKEDVDDEMALCPKCEFSFCVFCLHSWHGSRNLCGNSYTVKNEIVEEYSSEDTAPERKREMEMKYGRRTLQMAAADAVAEKLLDIAIAEESSNLKRCPSCRTVIQRTDGCNNMRCTVCFNFFCYLCGEALDKLDPYHHFSEPLSKCYARLFEGMPGIVAPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>C5DG85</accession>
    <name>C5DG85_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="11">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">KLTH0D03190g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="11"/>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002552865.1">
        <property type="nucleotide sequence ID" value="XM_002552819.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DG85"/>
    <dbReference type="FunCoup" id="C5DG85">
        <property type="interactions" value="855"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DG85"/>
    <dbReference type="GeneID" id="8295085"/>
    <dbReference type="KEGG" id="lth:KLTH0D03190g"/>
    <dbReference type="eggNOG" id="KOG0348">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_26_2_1"/>
    <dbReference type="InParanoid" id="C5DG85"/>
    <dbReference type="OMA" id="AVHIKAD"/>
    <dbReference type="OrthoDB" id="422663at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17949">
        <property type="entry name" value="DEADc_DDX31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025313">
        <property type="entry name" value="SPB4-like_CTE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13959">
        <property type="entry name" value="CTE_SPB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01178">
        <property type="entry name" value="DUF4217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 11" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="8 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="15">
        <location>
            <begin position="132"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="165"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="14">
        <location>
            <begin position="399"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="676"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="10">
        <location>
            <begin position="132"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="687"/>
            <end position="696"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAR22427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="716" mass="79156" checksum="DC184605E7551A73" modified="2009-07-28" version="1">MSDDDGMMLLNLDTTSSVDQTPGKVKVSGGRWKDRRKAKAMLNGKSVNSKDDRLVTSGSVKRPVSTEQGVEERKEQPARKQAKKVQIKATPADQQIVSSLFTSNRGIVTSINNNVHDENSGVGPSNAPLLQDSFEALGITSPLLQHLSEVMHITKPTRVQKMSIPTLLEADNDVFVHAQTGSGKTLAFLLPILSRILSMGARVDRQSGCFGMIITPTRELASQIHSVMLTLTKCCHYLIPCLLIGGERKKSEKARLRKGCNFIIGTPGRILDHLQNTQVIKEQLSSSLRYLVLDEGDKLMELGFEETITQIIKFLYEVPLNHFKYPELPKRIIHVLCSATLKGGVNKLGNLALQDYKLISSGRKQQEVNTVPDQLIQKIVMVPPKLRLVTLAAELNNITVRHLASETGETRRTIVFLSCSDSVEFHYAAFSSDSGHGRALVGDSVKVLSGNSTSLPCLADVSQPSVVCYKLHGSLSQQIRTATLKSFSSDSDATKGKHLIMFCTDVASRGLDLPCVSSVIEMDPPFAVEDHLHRIGRTARAGVSGESLLFLLPGEEEGYMDHIKPHHPQGWKLLKYDVDILKPAFMESKVQRSDGVSGKPISDEWDNNATTWHLNIERRVLGDPTFKEIAAKGYTSHIRAYATHLSQEKKFFSIKCLHLGHLAKSFALRERPKVMGGMNAKNSANGEQKKPKETSKSKMLRMAKKALDQSMSEFNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DGY3</accession>
    <name>C5DGY3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0D09240p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0D09240g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CU928168">
        <property type="protein sequence ID" value="CAR22675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553113.1">
        <property type="nucleotide sequence ID" value="XM_002553067.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DGY3"/>
    <dbReference type="FunCoup" id="C5DGY3">
        <property type="interactions" value="135"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DGY3"/>
    <dbReference type="GeneID" id="8295350"/>
    <dbReference type="KEGG" id="lth:KLTH0D09240g"/>
    <dbReference type="eggNOG" id="KOG1509">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040416_0_2_1"/>
    <dbReference type="InParanoid" id="C5DGY3"/>
    <dbReference type="OMA" id="VIGCDSV"/>
    <dbReference type="OrthoDB" id="10267058at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome D"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047429">
        <property type="term" value="F:nucleoside triphosphate diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00528">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029001">
        <property type="entry name" value="ITPase-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003697">
        <property type="entry name" value="Maf-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00172">
        <property type="entry name" value="maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43213">
        <property type="entry name" value="BIFUNCTIONAL DTTP/UTP PYROPHOSPHATASE/METHYLTRANSFERASE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43213:SF5">
        <property type="entry name" value="BIFUNCTIONAL DTTP_UTP PYROPHOSPHATASE_METHYLTRANSFERASE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02545">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52972">
        <property type="entry name" value="ITPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR22675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="251" mass="28375" checksum="A055BE15CDABCA96" modified="2009-07-28" version="1">MTLSMTFLSRFRSLRKIPLRCSLHQRCSMMSRVIKEISEKFEIVLASSSPRRYEIVTQVMGFADVKLMKPSFAEDHDKSLYAGNPTGYVHDTSKSKALGIIEDLKHTEETHASKPKLVICADTVVIDSDENIHEKPQRPELQLGNLLRFRESDGPVKVITSVTLIKWESPQDFTFTQFEEVSEVHFDSDFPLPILYDYVDSGDALDVAGGFKVQSFGGAMIKKINGDFFNVVGLPLNKTFKELYKAAFPLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHF9</accession>
    <name>C5DHF9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Aminopeptidase</fullName>
            <ecNumber evidence="11">3.4.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0E04026g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9 11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9 11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.-" evidence="11"/>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553657.1">
        <property type="nucleotide sequence ID" value="XM_002553611.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHF9"/>
    <dbReference type="STRING" id="559295.C5DHF9"/>
    <dbReference type="GeneID" id="8291806"/>
    <dbReference type="KEGG" id="lth:KLTH0E04026g"/>
    <dbReference type="eggNOG" id="KOG1046">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003705_0_1_1"/>
    <dbReference type="InParanoid" id="C5DHF9"/>
    <dbReference type="OMA" id="WNVWSQF"/>
    <dbReference type="OrthoDB" id="10031169at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09601">
        <property type="entry name" value="M1_APN-Q_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1730:FF:000002">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000006">
        <property type="entry name" value="Puromycin-sensitive aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024571">
        <property type="entry name" value="ERAP1-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034016">
        <property type="entry name" value="M1_APN-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF171">
        <property type="entry name" value="AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11838">
        <property type="entry name" value="ERAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 11" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="5 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 9 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3 11" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Aminopeptidase N-like N-terminal" evidence="14">
        <location>
            <begin position="12"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M1 membrane alanine aminopeptidase" evidence="12">
        <location>
            <begin position="247"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="domain" description="ERAP1-like C-terminal" evidence="13">
        <location>
            <begin position="537"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="322"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="321"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="325"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="344"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="10">
        <location>
            <position position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU364040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF11838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF17900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR23220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="870" mass="98330" checksum="6FA08A55F4E3C795" modified="2009-07-28" version="1">MTEKQTLPTHFQPLHYSIELDCLEANAKKFNGRVSIDFKVRQACNKVYLNTRDLEIDSASIVKRTREFIGLMAQDYDPVNEVTVLTFAMELRDDFTLVLNYRGLIQTNMTGFYQSEYSDATTGETKTMLSTQFEATDARRAFPCLDEPLLKATFDVSITASSELVVLSNMPSRHTEVSSDGKTTKHNFHRTPKMSTYLVAWALGEFEYIEGHTENRIYPANPGCGAERQKLPIRVYTSQGKSHQGAFALGVASKVIDYYSRLFDIPYPLPKLDLLCVEAYSHNAMENFSLITFRPTALLLEGDVASADPLALQKIAYVVSHEIAHQWFGNLVTMKWWDELWLNEGFATWVGYHAISEFFPEWDVPSLVMLDSHEVALQLDSLAESHPVKVHVKNAKDIDQVFDAISYLKGCSILEMISEYVGVEKFVGGVSLYLKRNVFSNATMEDLLGSIGEVCGCDILARLQNWILNIGFPLLKVQTADAGEVELCQERFLSTAVHSEDSTVWWIPLMTQGNLQNRKLELWEKSSSFKVPEEGLLHFNAAGFGFYRVSYEDKRHLYRICDNLEFIPSRGKIGLLSDVQTTESASVLLEVLSYFSRALKREDGYVWVMVYNVCASLLLLSYKSSSLEAFNKIKTFVLSLLEPLIDDALAYLCDPSSVSSNQSDAHRSSRTRFYDQALLVAGALSHEKVVQNCQEIFRRNLVTPATRKVVLSTILSQPKAPECLFDEVAAELDTATLAHKETVIAALGKVQNPALFQKSLQLIFRLQSMDVQFLTAAWATNPFIWEYVWPFVRNNYDSIHKHISANPTVIERFVRNVLNCLVGPSLKKDVERFFEGKDVAAFDRALHQGLEIIEKNTLYVKKNFESSASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHL0</accession>
    <name>C5DHL0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0E05192p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0E05192g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family. Cyclin AB subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553708.1">
        <property type="nucleotide sequence ID" value="XM_002553662.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHL0"/>
    <dbReference type="FunCoup" id="C5DHL0">
        <property type="interactions" value="613"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHL0"/>
    <dbReference type="GeneID" id="8291859"/>
    <dbReference type="KEGG" id="lth:KLTH0E05192g"/>
    <dbReference type="eggNOG" id="KOG0653">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020695_10_4_1"/>
    <dbReference type="InParanoid" id="C5DHL0"/>
    <dbReference type="OMA" id="ANDPFMV"/>
    <dbReference type="OrthoDB" id="5590282at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044772">
        <property type="term" value="P:mitotic cell cycle phase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20512">
        <property type="entry name" value="CYCLIN_CLBs_yeast_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000005">
        <property type="entry name" value="G2/mitotic-specific cyclin B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046965">
        <property type="entry name" value="Cyclin_A/B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004367">
        <property type="entry name" value="Cyclin_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048258">
        <property type="entry name" value="Cyclins_cyclin-box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02984">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001771">
        <property type="entry name" value="Cyclin_A_B_D_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01332">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00292">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3 5" id="KW-0195">Cyclin</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="7">
        <location>
            <begin position="260"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin C-terminal" evidence="8">
        <location>
            <begin position="353"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="7">
        <location>
            <begin position="357"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="54"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="152"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="15"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="76"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="92"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="152"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR23271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="487" mass="55387" checksum="622F67F9B9C27041" modified="2009-07-28" version="1">MSFAHNDNSQDSRPRTLKSTRTALNNVTNTTIAQEVKSANSSARSLGKVLRAARSKLQSDKTVGSLIPQIRREGTLSEIPPSSSSSTSLSSAGTIPASQVTSSELDKENMDPFASKLPSIEEEQQSQSLSSSREALQYSEHQTMLKDSDYASAMQSDLQAQQQQQRSSNILEPAADVSKKRPISTVVEQEEPKKFKVCAKGTEYEWEDLDAEDINDPFMVSEYVNDIFEYLHKLEIMTLPNRHDLYKHSNIRQNRDILVNWMVKIHNKFGLLPETLYLSLNIMDRFLCRELVQLEKLQLVGTACLFIASKYEEVYSPSVKHFAYETDGACDEDEIREGEKFILKTLEFNLNYPNPMNFLRRISKADDYDIQSRTLAKYLLEISVVDFRFIGVLPSLCAATAMFLSRKMLGKGKWDGNLIHYSGGYTREELAPVCNMVMDYLVQPVVHDEFFKKYASRKFMKASVISRQWAKKVVAHGYDIMTLHEPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHM4</accession>
    <name>C5DHM4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0E05544p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0E05544g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553722.1">
        <property type="nucleotide sequence ID" value="XM_002553676.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHM4"/>
    <dbReference type="FunCoup" id="C5DHM4">
        <property type="interactions" value="323"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DHM4"/>
    <dbReference type="GeneID" id="8291875"/>
    <dbReference type="KEGG" id="lth:KLTH0E05544g"/>
    <dbReference type="eggNOG" id="ENOG502QU0Q">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008754_0_0_1"/>
    <dbReference type="InParanoid" id="C5DHM4"/>
    <dbReference type="OMA" id="DIRYPKM"/>
    <dbReference type="OrthoDB" id="2264563at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046868">
        <property type="entry name" value="BAR_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046869">
        <property type="entry name" value="SLM1/RGC1-like_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31941:SF15">
        <property type="entry name" value="ACTIVATOR OF SKN7 PROTEIN 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31941">
        <property type="entry name" value="CYTOSKELETAL SIGNALING PROTEIN SLM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20400">
        <property type="entry name" value="BAR_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20399">
        <property type="entry name" value="PH_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="449"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="168"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="569"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="664"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="711"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1026"/>
            <end position="1047"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="8"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="168"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="572"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="730"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="756"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="772"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="794"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="810"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="874"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="904"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="952"/>
            <end position="963"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR23285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1047" mass="114148" checksum="865CED1CFE71F5C6" modified="2009-07-28" version="1">MSSAKQSGFHDTEPTQRDFLAPEREEDDASSLFSSDSGYLMDLADASEDTETILPKTDFHSPYYVNVPLPSQSGLTSDYTEPSQDSTSTGFVREYPTDILLDRFNKWRKILKGLANYLREVAYAQERFARINYGLRSTVKFSFLTDLEESTNRVIDPFQQRAKKQAGASAASLNSSLRTPSAPGEESPGGINSSQSALDVKNVPMAPGDNSSASSGFMKFGSGSIEDIQVVLKKYHASIANQQIKMSKEITTMVVPKLDDLRKDLQLKIKEIKELHGDFRTNIGEHIALTGQLLQKYMAAVEFMSTNSNDQKIFKLKNQKLKPKHDPYLLKLQLDLQLKRQLLEENYLQEAYINLQSSGMELEKIIFGEVQHTLQRYSALIGTSARISISNLCNELHQGVLSKPPAIEWDHFVGHHPKCLIDWKSTEPIPQPRKLSDIRYPKMKSSLAKCIKAGYLCKKSQILKNYNKAYFVLTSNYLHEFKSSNFFKLSQENSEKKDHGEVQTGSKKRAMVPVTSLSLNSAQLLEASEHKFSIRGVTSYNNFEKKSAETGKIISKSTSSIHKFLKSGSKLQPHKKDQHHHGSIAGLPSGAGAHGPSEDLDDVATWVFKAASSKPEDAKDLKKWVTEVKNLSSFMNSDERAKFIEEKILRAHNRASSMNLSKMAAESGNNGRRQDRLPSHKVSGTRSKPQFIHLGPQEMQDPALARAKVNTPAIDDNGNLITAGERRSSSLKSPSEVPSPHLSPTPTNHSGGGTTPGSSGHGSDTPMKQQGFVITSNGITPINRTPGSASKRNVSGVVPSGGSSDGSNSAPMNSPGSSSAPVNSPGSNSQTNFFGSNNSPIHSPGSGILPLNSPASHLSGGSGSGGYFAIPVRPSSQASTPGLGNPDSTAAAAQTQGSNVPRLRVNDRDMSQEKTDKNLPHSEQSTEKPAGSGRPAEGGPLSRGAPVHLRKTSNPGGISTFISEPHQTIYSNPRQSLSSQTLGSTNHQHVRKHKKNVSFGSLNSLLFSKKGGDVSNNNMTDYFMSGNRIQENDDPDALNVNQSLYRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>C5DHU4</accession>
    <name>C5DHU4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glycerophosphocholine acyltransferase 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0E07172g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GPC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553792.1">
        <property type="nucleotide sequence ID" value="XM_002553746.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DHU4"/>
    <dbReference type="FunCoup" id="C5DHU4">
        <property type="interactions" value="212"/>
    </dbReference>
    <dbReference type="GeneID" id="8291947"/>
    <dbReference type="KEGG" id="lth:KLTH0E07172g"/>
    <dbReference type="eggNOG" id="KOG2895">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018994_1_2_1"/>
    <dbReference type="InParanoid" id="C5DHU4"/>
    <dbReference type="OMA" id="YIDYYGK"/>
    <dbReference type="OrthoDB" id="406287at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006656">
        <property type="term" value="P:phosphatidylcholine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021261">
        <property type="entry name" value="GPCAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31201:SF1">
        <property type="entry name" value="GLYCEROPHOSPHOCHOLINE ACYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31201">
        <property type="entry name" value="OS01G0585100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10998">
        <property type="entry name" value="DUF2838"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="134"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="159"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="184"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="261"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="318"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="346"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="370"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR23355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="407" mass="48131" checksum="4AE53E2181D4A6CB" modified="2009-07-28" version="1">MSDIPDTLDTDSDEGSEPMKVTLANWVEFLDPLSFTAQSQYLPRKYLTRARRRLKSARPRQKLNQLKTTAQDLTPQLEDFKRRTIERLHSLDRPLESLFFHNSSRLEKWFYPFTLFHIFAIGFIIGKYPSWFHVYYTGMLVLLMPVRFYTYYKTNNHYYMADLCYYVNLMVLLFVWVWPDSVHLYQSCFAFTFGTLSFAVITWRNSLVIHSVDKITSCFIHIMPPLTMYTIHHGLGEDLKRARFPAASLAGSKKWNLKYNIFWTSLYYLLWQSAYHYFITLRKSSKIKSGQRATSFEYLTTHQFKNFWAAKLPEPWPMFFYILFQYCYQLGTMMLCGIWFNYRGAAGAFLTFIYLCAAKNGATYYIDYYGKKFEKEVAKLKSEVEDLQQQLSQKANSSSNNYSGVLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>C5DI46</accession>
    <name>C5DI46_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0E09636p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0E09636g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553894.1">
        <property type="nucleotide sequence ID" value="XM_002553848.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DI46"/>
    <dbReference type="GeneID" id="8292058"/>
    <dbReference type="KEGG" id="lth:KLTH0E09636g"/>
    <dbReference type="HOGENOM" id="CLU_2027144_0_0_1"/>
    <dbReference type="InParanoid" id="C5DI46"/>
    <dbReference type="OMA" id="LANEFKW"/>
    <dbReference type="OrthoDB" id="4035593at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="126" mass="14171" checksum="B7E56EE25E050338" modified="2009-07-28" version="1">MSELLRRITRQISPHHNPEGITWGKSRALLDQARQSTVAQVSKGCQKDMLATALAAKLADEFKEEVVHVERAAADPPRPRKINSKRSERPEEIKPQDKRKSRARLTSSRVAELVQRASGTEEGMYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>C5DI65</accession>
    <name>C5DI65_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glucosidase 2 subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0E10054g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002553913.1">
        <property type="nucleotide sequence ID" value="XM_002553867.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DI65"/>
    <dbReference type="FunCoup" id="C5DI65">
        <property type="interactions" value="584"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DI65"/>
    <dbReference type="GeneID" id="8292077"/>
    <dbReference type="KEGG" id="lth:KLTH0E10054g"/>
    <dbReference type="eggNOG" id="KOG2397">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_419754_0_0_1"/>
    <dbReference type="InParanoid" id="C5DI65"/>
    <dbReference type="OMA" id="CCDCSDE"/>
    <dbReference type="OrthoDB" id="28322at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017177">
        <property type="term" value="C:glucosidase II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006491">
        <property type="term" value="P:N-glycan processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.130.10">
        <property type="entry name" value="Mannose-6-phosphate receptor binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039794">
        <property type="entry name" value="Gtb1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009011">
        <property type="entry name" value="Man6P_isomerase_rcpt-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044865">
        <property type="entry name" value="MRH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036607">
        <property type="entry name" value="PRKCSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028146">
        <property type="entry name" value="PRKCSH_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12630:SF1">
        <property type="entry name" value="GLUCOSIDASE 2 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12630">
        <property type="entry name" value="N-LINKED OLIGOSACCHARIDE PROCESSING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12999">
        <property type="entry name" value="PRKCSH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13015">
        <property type="entry name" value="PRKCSH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50911">
        <property type="entry name" value="Mannose 6-phosphate receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51914">
        <property type="entry name" value="MRH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005667957" description="Glucosidase 2 subunit beta" evidence="5">
        <location>
            <begin position="19"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="domain" description="MRH" evidence="6">
        <location>
            <begin position="527"/>
            <end position="655"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR23476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="665" mass="75036" checksum="D56BFE7D26A34B3B" modified="2009-07-28" version="1">MPALAYYAVALCCTFAGARQVNGVPGSLQHLYEPMQTDPTKWACLGDPSIVLNYTQINDDFCDCPDGSDEPGTSACGALSRYYCENKGFAPKFVAGFKVNDGVCDCCDCSDENENIRSALSSDNCSQLRAQFERALKEELNEQELGSQALKMLEREYGIYAEETPLEDKKGALRKAKSETGESKEALEACVNELAQAKETYKDTLQRENPLLLQLEEIDLQYVTSVIHSLFAKAESTSRAREALVKILDDLRNGYNKNLNDEVVNRNVKTYTDYAPTEHEELNSSPSLDRTQREQINAYLNEELPDMFLRGSTHIPPAAIVGKFSMARTLIKVRAKQQKKDKETLEFFTKIMDDVSQNYNVNFQDSSVKASVISYRDFLAKNPHLFEPLDIPSEFTEKMNTLEELLNDVSTKILTPSAQSQGFLGVSFDDLAQKLRSFFGDQTNSLEQLKSAIVSLEQKCSTLRTEYRSQLQNFKLLESQVIENDSSSNSVNSETFKAMDELLSKLSPSCVEQKIDNYIYQICLNQEDGVVFQREDKPAGNSVLIGRFAGFSFDAKSTWDRYMDDLRIKYSESDLVSHLESDLDNFHTEWLVGNLPEKNSGLVVDYKQGDRCWNGPSRSARLYFECSDDFKLKSVQEPTRCHYAFHLSGPLGCSTDFAYAAPDWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>C5DI73</accession>
    <name>C5DI73_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0E10230p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0E10230g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="CAR23484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002553875.1"/>
    </dbReference>
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    <dbReference type="FunCoup" id="C5DI73">
        <property type="interactions" value="75"/>
    </dbReference>
    <dbReference type="GeneID" id="8292085"/>
    <dbReference type="KEGG" id="lth:KLTH0E10230g"/>
    <dbReference type="eggNOG" id="ENOG502RZHH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_487610_0_0_1"/>
    <dbReference type="InParanoid" id="C5DI73"/>
    <dbReference type="OMA" id="QENYGYQ"/>
    <dbReference type="OrthoDB" id="4069015at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="159"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="33"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
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        <location>
            <begin position="171"/>
            <end position="188"/>
        </location>
    </feature>
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        <location>
            <begin position="189"/>
            <end position="207"/>
        </location>
    </feature>
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            <begin position="311"/>
            <end position="327"/>
        </location>
    </feature>
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            <begin position="348"/>
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        </location>
    </feature>
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        </location>
    </feature>
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            <begin position="497"/>
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        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="547" mass="59037" checksum="F973D4ABF17B18E1" modified="2009-07-28" version="1">MVLGIHRKPQKKPDLSRYDYHYQQKDSTGGGHALSAAAAGAAGRSRSLVHPPARANHGVSGTPYTRSYSMRHPSSNGTSRRRPATNGALGSRTSSLLGSAGQTRRAVSGPTTGSRTNSLASPSRSITVKTTEVRDPMGRTQSITRQTVRKINGYEYVETTTTSTTTSPLGDAERHFDEFTSDFTHGETLEEEDNEDGDAENDEDGTEYSDAIDVLPSPPPQRAQRQASPREAKPKRSALPRKALTEQEQYAKALAAAQRKVYGDRAEQKVAAGSMGPTGTSGRKSRRVSSLRDPVVPSEPARHEGPAPGSTRRNTMRRSFSQVISPSRRTEAPADAPVPSPQTQPQRRMNDDEIYAKALEIARKKFAADSMEEPREIPEAQSAQKPDLYQEAPVSTQQPASDAAQRGPPRSRVKSFFSKVAQFSQENYGYQDKKNEPVANASTQASRPAGTGSSAQAEPQTSTLYSMDSNVSRRSMRGSASGPLQSSAVSSEPVRIAAATPPETEETVPAPQVMKATTANSSPAATGPVPTKQVKVGFFQRLFKRSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5DI85</accession>
    <name>C5DI85_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0E10516p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0E10516g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
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            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="STRING" id="559295.C5DI85"/>
    <dbReference type="GeneID" id="8292098"/>
    <dbReference type="KEGG" id="lth:KLTH0E10516g"/>
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    </dbReference>
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    <dbReference type="InParanoid" id="C5DI85"/>
    <dbReference type="OMA" id="CARSAYD"/>
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    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
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        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Chitin_Synth_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006597">
        <property type="entry name" value="Sel1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46430:SF3">
        <property type="entry name" value="ACTIVATOR OF C KINASE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46430">
        <property type="entry name" value="PROTEIN SKT5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08238">
        <property type="entry name" value="Sel1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00671">
        <property type="entry name" value="SEL1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
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        <location>
            <begin position="53"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="194"/>
            <end position="224"/>
        </location>
    </feature>
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        <location>
            <begin position="56"/>
            <end position="68"/>
        </location>
    </feature>
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        <location>
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            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="114"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="132"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="200"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="211"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR23496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="632" mass="70067" checksum="CA7E15797CC7501A" modified="2009-07-28" version="1">MAGTSKQYLYDTHINMYPPSRGGMSAQRNIANSPSRIVPYPVEDIQQPMSPVALQSAAATSPATSTHTLVGSKRSPPPPNVRYDQPFTFQNGEQNLGGTPPPDLVSGLRRFDSATANNSSTDTFQSASDVSAPPPATATAMPPPPYEETENQQFLREKLYRTASDLSSGESGQEAGSRKPLQNQPLQNQHLQNQPLPEAQGSQYRTLDQGTPRKAERALPRELQRDLSQYSDEALYFYRVYQVTVDDSPNFTPKVQMKWCETLLTYSFNTEFVSNYNINSNKLKPRLTPEEALKNQKIILEHALKVLTKLITLQYGPALYLMATLYSHQPYLDIKIQAVIVRNDAKALDYYTRAAALNESEACYRAGVCYEYQRGTPTELDKAQCLQKAVYFFDRGATQCGNTACMYKLAMLLLHGVNEKYSAQAVLQQDVKAAMHWFKKAAAESAPTNGAVGSPQAMYELAKIYERDGLQPALQERLEAAGISRDATRALQYYHRCATLCSYPLAQWRLGQCYEFGQLNLPVAANKSIAWYAKAAMAQPRGNPMAMMALSGWYLTGATGVLKPNNREAYNWARKACQAADGKLARAEYALAFYSENGIGCTPSLAEAREHYERAAAAGHAKARDRLRAGDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIC2</accession>
    <name>C5DIC2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0E11374p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0E11374g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002553924.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIC2"/>
    <dbReference type="FunCoup" id="C5DIC2">
        <property type="interactions" value="17"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIC2"/>
    <dbReference type="GeneID" id="8292137"/>
    <dbReference type="KEGG" id="lth:KLTH0E11374g"/>
    <dbReference type="eggNOG" id="ENOG502S09K">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110375_0_0_1"/>
    <dbReference type="InParanoid" id="C5DIC2"/>
    <dbReference type="OMA" id="YAYVIVK"/>
    <dbReference type="OrthoDB" id="5580261at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018811">
        <property type="entry name" value="MRX11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28002">
        <property type="entry name" value="MIOREX COMPLEX COMPONENT 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28002:SF1">
        <property type="entry name" value="MIOREX COMPLEX COMPONENT 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10306">
        <property type="entry name" value="FLILHELTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR23533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="216" mass="23946" checksum="CA4063B0351ED661" modified="2009-07-28" version="1">MSLAFTSLGLSPASRLYAKGGSIALGLLRPSAVGVERCRCYSKKASSSNTVSEKAVGPAKDPSNKIYKLISKSKLLTRLSKRPAIGHYFDKLAETSPVSTISSFLILHEITAIVPLFGLWWVLYSLNLNEQYELPLYFKELLDQCGDSIEKLVGDRSQGFDRNRLVLSGAISYAIVKILYPARVLFSLWAAPYFFKWFLGPFKKLGSLLRPGKKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIP5</accession>
    <name>C5DIP5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">KLTH0E14124p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0E14124g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SPS2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554093.1">
        <property type="nucleotide sequence ID" value="XM_002554047.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIP5"/>
    <dbReference type="FunCoup" id="C5DIP5">
        <property type="interactions" value="106"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DIP5"/>
    <dbReference type="GeneID" id="8292262"/>
    <dbReference type="KEGG" id="lth:KLTH0E14124g"/>
    <dbReference type="eggNOG" id="ENOG502QUZC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035846_0_0_1"/>
    <dbReference type="InParanoid" id="C5DIP5"/>
    <dbReference type="OMA" id="DKFQCED"/>
    <dbReference type="OrthoDB" id="536881at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.20.20">
        <property type="entry name" value="Receptor L-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051648">
        <property type="entry name" value="CWI-Assembly_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036941">
        <property type="entry name" value="Rcpt_L-dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31018:SF3">
        <property type="entry name" value="RECEPTOR PROTEIN-TYROSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31018">
        <property type="entry name" value="SPORULATION-SPECIFIC PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0134">Cell wall</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950390" evidence="12">
        <location>
            <begin position="20"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="369"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="369"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR23656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="422" mass="42940" checksum="E4441B3750560C89" modified="2009-07-28" version="1">MQYKTGVATAALLSATALAANSTTSVPSSCSIKSGSTVTAQAQLDQYSGCQSLVGDLNIGGDSLTGSASLANVEEIDGSLNVVNATQLVSFAADKLSSISGSLSMKQLTLLQSASFGSLQEVDTIQFITLPAISSIATNLKSLNSLEISDTNLESIDGFNNLETVTDFNVNNNKNLAKIDSKLKSVSDSLQVGYNGDEADVKFDNLVWANNITLRDVSSASFAKLTKVNQSLGFINNSIESLNLSVGTVGGTLTVVSNDDLTDLDFSNLTSVAGGVVVANNSKLSNIDGLSKLQTIGGALTVIGNFSVLDLDSLKSVKGGADVETKSSNYTCDSLKKLQKNNAIQGDAFVCKNGATSTSIKLSSASGSETASESSASSGTKTATASTISSKSSSSKSSGGAGAQMAPVSFMGAVAAVAVALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>C5DIV2</accession>
    <name>C5DIV2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0E15422p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0E15422g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928169">
        <property type="protein sequence ID" value="CAR23713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554150.1">
        <property type="nucleotide sequence ID" value="XM_002554104.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DIV2"/>
    <dbReference type="GeneID" id="8292321"/>
    <dbReference type="KEGG" id="lth:KLTH0E15422g"/>
    <dbReference type="eggNOG" id="ENOG502SB1E">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1816148_0_0_1"/>
    <dbReference type="InParanoid" id="C5DIV2"/>
    <dbReference type="OMA" id="CDLEIHC"/>
    <dbReference type="OrthoDB" id="4031013at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome E"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR23713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="142" mass="15923" checksum="2ADEEE2E20E48F78" modified="2009-07-28" version="1">MVRTRLFQRCASANIEIENGLLGVCSMHLRAPHKRIVGIGSCTARVTGTSWGALYYNEEQDLAKKKFKLYCVIEQESLRIDFVELPVTGDDDHGPPWKDPLPEDPEYEFPVLVFQGSRPGSLDNDLQPFAGVMVLEEVDEVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJ29</accession>
    <name>C5DJ29_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">phosphoinositide 5-phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.36</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0F13002g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the synaptojanin family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the central section; belongs to the inositol 1,4,5-trisphosphate 5-phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.36" evidence="4"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554755.1">
        <property type="nucleotide sequence ID" value="XM_002554709.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJ29"/>
    <dbReference type="FunCoup" id="C5DJ29">
        <property type="interactions" value="47"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJ29"/>
    <dbReference type="GeneID" id="8292978"/>
    <dbReference type="KEGG" id="lth:KLTH0F13002g"/>
    <dbReference type="eggNOG" id="KOG0566">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003016_2_1_1"/>
    <dbReference type="InParanoid" id="C5DJ29"/>
    <dbReference type="OMA" id="KWWIGNG"/>
    <dbReference type="OrthoDB" id="405996at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043813">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004439">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000029">
        <property type="entry name" value="Inositol polyphosphate 5-phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046985">
        <property type="entry name" value="IP5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000300">
        <property type="entry name" value="IPPc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002013">
        <property type="entry name" value="SAC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200">
        <property type="entry name" value="INOSITOL 5-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200:SF269">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE 5-PHOSPHATASE INP51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22669">
        <property type="entry name" value="Exo_endo_phos2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02383">
        <property type="entry name" value="Syja_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00128">
        <property type="entry name" value="IPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50275">
        <property type="entry name" value="SAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SAC" evidence="11">
        <location>
            <begin position="148"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="863"/>
            <end position="889"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR24318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="936" mass="106540" checksum="136C171CD6107133" modified="2009-07-28" version="1">MKLFIRKEPRSLALLSNGYVLVLEKSRISQSQQESKGGQPTAVVESMHETSLSDHGFVEVKTSMFNGLLGLICVNNYLFLGLITNVQKVAFPRWKVIEGGQREDIESIFQILNVEFYCLNSDCFDFALSDLSDHGTDKVSSEHPCADLKKLFSDGSFYYSRDFDLSNTLQERGLNNGLDYAIDNQDQKFIWNSNMISEVTQLRNRITALERSLFDGALFLTFVVRGFCKTILMADVAAPMTLTIISRISTENKENIFDLQGIDKQGHVANFIESEVIFANEKYIFAYVQTSGNVPLSWEVSEGQILHGKKIKLVEDPACFQVAFDKHFDALASKYGVVSVLNLVKPKSESQEILSNAYRKCADNKDVRLTNVEYGSDILIKQSHKLIYLLKQDIYEFGAFVYDISKGIYVSTQTGCLRISAFDSIERPNLVEKCVSREVIEVTTSELGGLEITSNFLSTHDKLWDDNKFWLGRLYHDNFKNLNKFKKVYWKLFSSSCRVSLYDPLHAHISKLMRKMRKEFTYQKNISIFAGTFNVNGRMYPDDIGSWLFPPSVDMGNLPDVYVLGFEEVVELSPGHMLSTDPYAKQFWEKKILGTLNSVGNKKFIQVWGGQLGGVLLLLFISESEYGKVKHLEGDVKKTGFGGMSSNKGAVAVSFTYSTTKFCLLVSHLAAGLENVDQRHHDYKTIVKNIRFSRNLTIKDHDVVIWMGDFNYRISLPNDEVRRRVSGNDYEALFERDQLNQQMIAGESFPYYNEMEIKFPPTYKFDPGTKTYDTSEKLRIPAWTDRILSRGDSVRQASYGSAENIIFSDHRPVFATFNSTVNVVDEKLKAKLATEIYERLTEQLSDLTDEERFTVLNSGELFPDKSESDEPVAPEIRKGRKLPPPSSAAKKWWIGSSKQVKVLLDVDPTKFMINPSRPVNPFVTDEEPFFVRREST</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJ39</accession>
    <name>C5DJ39_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0F13244p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0F13244g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554765.1">
        <property type="nucleotide sequence ID" value="XM_002554719.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJ39"/>
    <dbReference type="FunCoup" id="C5DJ39">
        <property type="interactions" value="668"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJ39"/>
    <dbReference type="GeneID" id="8292989"/>
    <dbReference type="KEGG" id="lth:KLTH0F13244g"/>
    <dbReference type="eggNOG" id="KOG3207">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017716_5_1_1"/>
    <dbReference type="InParanoid" id="C5DJ39"/>
    <dbReference type="OMA" id="SEESHMF"/>
    <dbReference type="OrthoDB" id="5273213at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.190">
        <property type="entry name" value="CAP Gly-rich-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036859">
        <property type="entry name" value="CAP-Gly_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000938">
        <property type="entry name" value="CAP-Gly_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15454">
        <property type="entry name" value="NISCHARIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01302">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01052">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74924">
        <property type="entry name" value="Cap-Gly domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50245">
        <property type="entry name" value="CAP_GLY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CAP-Gly" evidence="4">
        <location>
            <begin position="33"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR24328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="499" mass="56318" checksum="E67DB5F963C55C84" modified="2009-07-28" version="1">MIEIGDRLFVDGHTCSVLFIGYIPAWKDSRAFGLEWDEPKRGKHSGYLNGVEYFKTRIPNAGSFVKESKLLYAIQSRKSFVEAVNNVYLRGLENREDVFFGFKKVELFGLNVLASKNQDLENLKYLDLSAKLIASSGETKDLLALNFRFKSLTQLNLSSNLFKDFSEVSRVLSGIPHLRSLDISNNRFEVYSNAADAEQVQSLEELRANFCQLSTEGIANLLSYFPNLKRLELSGNCARELDSISFPASLSELSLAENCLSTMPLNIFEASVVRLNVSSNKIYSLLGGVFPKIEALDLSYCCIDRWDSIDEICTNFPNMKDFRINGNPLTMDESDDNVFLQLIGRNRNIDRLNGSFLTEKVRYDAEIYFMAQVSTQNATINKAGRQWNYLLQKHGPVSRGGGSIGSGTIFEILTVDLEFEGYKVRELSMLPSYSIRFLKTTISRTLSIPASEFNLYYVLEGGTQQEFSFEFSPISMFNIKSGDRICVDLKNAKRRSSAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJ49</accession>
    <name>C5DJ49_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0F13486p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0F13486g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MNN1/MNT family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554775.1">
        <property type="nucleotide sequence ID" value="XM_002554729.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJ49"/>
    <dbReference type="FunCoup" id="C5DJ49">
        <property type="interactions" value="29"/>
    </dbReference>
    <dbReference type="GeneID" id="8292999"/>
    <dbReference type="KEGG" id="lth:KLTH0F13486g"/>
    <dbReference type="eggNOG" id="ENOG502RZ48">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015387_1_0_1"/>
    <dbReference type="InParanoid" id="C5DJ49"/>
    <dbReference type="OMA" id="FFWLGQL"/>
    <dbReference type="OrthoDB" id="430354at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000033">
        <property type="term" value="F:alpha-1,3-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022751">
        <property type="entry name" value="Alpha_mannosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31392">
        <property type="entry name" value="ALPHA-1,3-MANNOSYLTRANSFERASE MNN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31392:SF1">
        <property type="entry name" value="ALPHA-1,3-MANNOSYLTRANSFERASE MNN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11051">
        <property type="entry name" value="Mannosyl_trans3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR24338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="623" mass="72374" checksum="900249DB01ECDA3C" modified="2009-07-28" version="1">MMLRVRKTGFRRAVVFFGFFLVCLLIARTSRKQRGFSRASLEQFAKLCRESSQRVALNDSPFSDFLKPWWRVSMARQDWREFKSQNLQQKCRYYFDSVHLKDPQWTNNAVRILFNSDNANNAKISNVIERVRVFDDCFISGDLSLNQVIPQKRDVMGFHHRVFPFLRDFRKPEELWPTIVDLKTRRELRKGVVPHYSAPEDGAFRMDNSKSFWRNWNAFSTGRGLVFSLGERHLDPFIRHLKILDHLGNKLPIQIVQRENELSEQFLNALSSFLQRSHQRVYYINCGAILEPLYGLEMTYFVNKWIASIFNTFSEVILMDADVIPFIPLEEFFDDDQYNRSGVLLYKDRDMPGETTFDYCIESFNYLMPSAEATILMNHTMKVNGSAVRPDSTIFSNEEEHIYYRFFYNKTLHNVDSGLVVFRKPEKLIGLLISLFMNLDPRLSRCVYGDKELFWLGQLFGGQDYTIDPIDGSVVGPLVTKLSEETGEKEYEICATQMSHSSRDKRLLWTNGGLKKCKTPGAAHMDFDRSPDYFKSQYSSEEDLQNVYDQALQLEALIIPDIKSHPWVKAQECLEYTYCASLSIGDMQSALSNGALLVFDGDDSKDFNEISNIWNIDLIPQGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJ53</accession>
    <name>C5DJ53_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0F13596p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0F13596g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554779.1">
        <property type="nucleotide sequence ID" value="XM_002554733.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJ53"/>
    <dbReference type="FunCoup" id="C5DJ53">
        <property type="interactions" value="36"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJ53"/>
    <dbReference type="GeneID" id="8293004"/>
    <dbReference type="KEGG" id="lth:KLTH0F13596g"/>
    <dbReference type="eggNOG" id="ENOG502SBHA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1907071_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJ53"/>
    <dbReference type="OMA" id="IAHEISW"/>
    <dbReference type="OrthoDB" id="5529571at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070096">
        <property type="term" value="P:mitochondrial outer membrane translocase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045040">
        <property type="term" value="P:protein insertion into mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013262">
        <property type="entry name" value="OMP_MIM1/TOM13_mt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28241">
        <property type="entry name" value="MITOCHONDRIAL IMPORT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28241:SF1">
        <property type="entry name" value="MITOCHONDRIAL IMPORT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08219">
        <property type="entry name" value="TOM13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="142" mass="15648" checksum="B9127AF22520A8F6" modified="2009-07-28" version="1">MVGQLASKVPEELASVAIPGPTAPTGVEEGDQQDEPLQKTEEAVEDESYSNASGLPSAQSNSCMKILYFAGSCSVNLILPFFNGLMLGFGELIAHEISWKFNWFRRSNLGYRIYPESRKLTARQQTELDSQKGASRKARAFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJ94</accession>
    <name>C5DJ94_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0F14586p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0F14586g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CBF/MAK21 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554820.1">
        <property type="nucleotide sequence ID" value="XM_002554774.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJ94"/>
    <dbReference type="FunCoup" id="C5DJ94">
        <property type="interactions" value="702"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DJ94"/>
    <dbReference type="GeneID" id="8293049"/>
    <dbReference type="KEGG" id="lth:KLTH0F14586g"/>
    <dbReference type="eggNOG" id="KOG2154">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015945_1_0_1"/>
    <dbReference type="InParanoid" id="C5DJ94"/>
    <dbReference type="OMA" id="KHPTCMI"/>
    <dbReference type="OrthoDB" id="10263185at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030692">
        <property type="term" value="C:Noc4p-Nop14p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005612">
        <property type="entry name" value="CCAAT-binding_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027193">
        <property type="entry name" value="Noc4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12455">
        <property type="entry name" value="NUCLEOLAR COMPLEX PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12455:SF0">
        <property type="entry name" value="NUCLEOLAR COMPLEX PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03914">
        <property type="entry name" value="CBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCAAT-binding factor" evidence="2">
        <location>
            <begin position="310"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR24383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="533" mass="61435" checksum="323B2E6171F49F3B" modified="2009-07-28" version="1">MKTSVDDIKATAKLLLSSNDRKNYNSIVKLVGQYSHLQLAEKVQFAEQEPTARCLTVSLYQVFRKLFFRGQLNPSKATTDKEKLFFQWCRKAYGSFKSKLLHCISSLPFESSLALDCLDVYMKLIEQEAIFFASQDGAPFFPNKTLKALIRALLESNIPGSIDATDGQSKSAVIQEFSEKYYQKFVDVQYYFQAELVQLTADGDLDQLDNTQLMAKWLCIMNHDNHYCNENADLEVFVAQPPQAIENEGQYKSNLEKNWLQFLNLRGLPGTQYKTTLLILHKRVIPYFQTPTKLMDFLTDSYDLGDDVLSLLALNGLFELMRKYNLEYPNFYEKLYQLITPSLMHVRHRSRFLRLTDLFLSSTHISVNLVASFIKRLARLTLDSPPAAIVSVIPFVYNLIKKHPTCMIMLHDPDFVANPFADANELAQLKSRKSQYVDPFDMNESNPENTHAIDSSLWELESLTSHYHPNVATLAKIFSQPFNKHNYNMEDFLDWSYDSLLQAEMSRKLKILPALEFEERGAPLGDYVKLVEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJ98</accession>
    <name>C5DJ98_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0F14696p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0F14696g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554824.1">
        <property type="nucleotide sequence ID" value="XM_002554778.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJ98"/>
    <dbReference type="FunCoup" id="C5DJ98">
        <property type="interactions" value="76"/>
    </dbReference>
    <dbReference type="GeneID" id="8293053"/>
    <dbReference type="KEGG" id="lth:KLTH0F14696g"/>
    <dbReference type="eggNOG" id="ENOG502S1BK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111702_0_0_1"/>
    <dbReference type="InParanoid" id="C5DJ98"/>
    <dbReference type="OMA" id="PEEYQME"/>
    <dbReference type="OrthoDB" id="4035738at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="14"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="219" mass="24034" checksum="9BCA295C69EA9A75" modified="2009-07-28" version="1">MCSNRNDGDEASSELELSSLSSSDSFEPRAFEGPPSEIKSLEPEGSVQASPSIDSKDFHRVKSNGTLRKQDSNAIEQIMTHNATEGRSETVESLKKNGLNLQKKAIPDINNPAANYKNCAFPEEYQMETDTGLVKAQTLHGLNRLESRTSGRSGASQKKSIRTANSTTNSDLASANNTINGLDGEKLRRAVEKNQKKIDKYQKHKSSGGLRRFLGKIFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DJD5</accession>
    <name>C5DJD5_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ceramide glucosyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.80</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Glucosylceramide synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">UDP-glucose ceramide glucosyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">UDP-glucose:N-acylsphingosine D-glucosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">KLTH0F15554g</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyltransferase 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.80" evidence="5"/>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554861.1">
        <property type="nucleotide sequence ID" value="XM_002554815.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DJD5"/>
    <dbReference type="STRING" id="559295.C5DJD5"/>
    <dbReference type="GeneID" id="8293092"/>
    <dbReference type="KEGG" id="lth:KLTH0F15554g"/>
    <dbReference type="eggNOG" id="KOG2547">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030898_1_0_1"/>
    <dbReference type="InParanoid" id="C5DJD5"/>
    <dbReference type="OMA" id="IVWIIDC"/>
    <dbReference type="OrthoDB" id="1483400at2759"/>
    <dbReference type="UniPathway" id="UPA00222"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008120">
        <property type="term" value="F:ceramide glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006679">
        <property type="term" value="P:glucosylceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025993">
        <property type="entry name" value="Ceramide_glucosylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12726">
        <property type="entry name" value="CERAMIDE GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12726:SF0">
        <property type="entry name" value="CERAMIDE GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13506">
        <property type="entry name" value="Glyco_transf_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002948771" description="Ceramide glucosyltransferase" evidence="16">
        <location>
            <begin position="27"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="54"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="420"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="451"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00019988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAR24424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="547" mass="62353" checksum="393409E670C1479E" modified="2009-07-28" version="1">MPGTRVLSIMIPGLVLFLSLVSPSLESVIADSKFTQDNSEKRRYGKDPVSGLQKFLALVCLIWYVTVLALAYSGWIEIVRKFRGPRGLADAGDSREPVSIIRPCKGIDTEMASCLESSILQAYATDRFEVLFCVESPQDPCIPVIKGLLEKYPDYDLKLLVGINGSEDHYGPNPKINNLSKAYRHAKYDIIWVLDSNVWCSPGTLSRSVASLTKSLDNGTKTRNRPVVLTHHVPLAISVAQSPLSLPLSARLDEMFMFSSHAKFYVGFNKVSIAPCVNGKSNLYRRSDLDKAVEYVGQATRRTDLFRDPQIQQDARMIALKNKASQDDHHLPFTEYVVVANKLQSRDVPAEEQQHHHGIEFFSTYIGEDNMIGTALWDMLGGRTGMTMDAVVQPLRFGAKDEGLRNFINRRVRWLRVRKYMVLAATLLEPTTESIVIGFMGAFWASRLLHLRFRLFFCMHLLIWCLTDWIQYCILSKVVFMDDQIRELPAFLSQAGRRRSFWDWLRIWVLREILALPIWIKAMCGSVIDWRNKPFRIKPDLTAEELQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-05-31" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5DK07</accession>
    <name>ZWINT_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Outer kinetochore KNL1 complex subunit KRE28</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Spindle pole body component KRE28</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">KRE28</name>
        <name type="ordered locus">KLTH0F00836g</name>
    </gene>
    <organism>
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Acts as a component of the outer kinetochore KNL1 complex that facilitates microtubule-kinetochore interactions and the spindle assembly checkpoint. Kinetochores, consisting of a centromere-associated inner segment and a microtubule-contacting outer segment, play a crucial role in chromosome segregation by mediating the physical connection between centromeric DNA and spindle microtubules. The outer kinetochore is made up of the ten-subunit KMN network, comprising the MIS12, NDC80 and KNL1 complexes, and auxiliary microtubule-associated components; together they connect the outer kinetochore with the inner kinetochore, bind microtubules, and mediate interactions with mitotic checkpoint proteins that delay anaphase until chromosomes are bioriented on the spindle.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the KNL1/SPC105 complex composed of SPC105 and KRE28. Part of the ten-subunit outer kinetochore KMN network that includes the KNL1, MIS12 and NDC80 complexes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
            <location evidence="1">Centromere</location>
            <location evidence="1">Kinetochore</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the KRE28 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554245.1">
        <property type="nucleotide sequence ID" value="XM_002554199.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DK07"/>
    <dbReference type="SMR" id="C5DK07"/>
    <dbReference type="FunCoup" id="C5DK07">
        <property type="interactions" value="34"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DK07"/>
    <dbReference type="GeneID" id="8292436"/>
    <dbReference type="KEGG" id="lth:KLTH0F00836g"/>
    <dbReference type="eggNOG" id="ENOG502S19U">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062083_0_0_1"/>
    <dbReference type="InParanoid" id="C5DK07"/>
    <dbReference type="OMA" id="GNEIDEC"/>
    <dbReference type="OrthoDB" id="4065660at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0180019">
        <property type="term" value="C:Knl1/Spc105 complex"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031619">
        <property type="term" value="P:homologous chromosome orientation in meiotic metaphase I"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905325">
        <property type="term" value="P:regulation of meiosis I spindle assembly checkpoint"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031361">
        <property type="entry name" value="Kre28"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17097">
        <property type="entry name" value="Kre28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0137">Centromere</keyword>
    <keyword id="KW-0158">Chromosome</keyword>
    <keyword id="KW-0175">Coiled coil</keyword>
    <keyword id="KW-0995">Kinetochore</keyword>
    <keyword id="KW-0539">Nucleus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000408563" description="Outer kinetochore KNL1 complex subunit KRE28">
        <location>
            <begin position="1"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="6"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="131"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q04431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="362" mass="41242" checksum="383E44AA4346547D" modified="2009-07-28" version="1">MADDPTINGLELQLNAAEEHATALTEEALQNQDLHYKEVVEQLKRSVEQLVEDNESLFTAVDLPPEVDPTCISGRILDLAQLTQQLKSTHLQQETLDNFLRYTISSTDILQLESESDERYASVSRAVSQLQDNDIIQLDSEVDQIKQDIRKAGQTIADQREALNELCLETGNLADECHTLLSELEEATRTREMVEKQAAVEVTNDHPVEEMYASWQSLKEELQQESHLRRHLNQLKQSKASLEAILGTKNGNEHKDTNVMQEYASYDAFIRFWISKFTNKEMENLEVFPRSNKFQFTHRGTDVVISLGPRGISRVELYGKGIPLEKIAAARKDVNEEASRGEELYMSINRIIDKIKEHTTVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>C5DK39</accession>
    <name>C5DK39_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0F01540p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0F01540g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554277.1">
        <property type="nucleotide sequence ID" value="XM_002554231.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DK39"/>
    <dbReference type="FunCoup" id="C5DK39">
        <property type="interactions" value="208"/>
    </dbReference>
    <dbReference type="GeneID" id="8292468"/>
    <dbReference type="KEGG" id="lth:KLTH0F01540g"/>
    <dbReference type="eggNOG" id="ENOG502QVI6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_529929_0_0_1"/>
    <dbReference type="InParanoid" id="C5DK39"/>
    <dbReference type="OMA" id="HTDGWCL"/>
    <dbReference type="OrthoDB" id="5340910at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="124"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="502"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="333"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="377"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="383"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR23840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="585" mass="63878" checksum="B25C073066DD78BC" modified="2009-07-28" version="1">MPASATSSTASQTLLLQPSVKGTVVESVYVEVYVLPGQTKPFSSSTASVRTLSILNRYQSATSDSSASRTSRTSQQTRSTQTLLQLMQSASSSTTLVQSASVSPASSEIVDKISAGQNASSATIALSIGLPLGIFCLAFFLIFALLWLRRRSAVRPPQDPFAKSPADVASNHQTPWKRFVDRCLAPKESTHSHSFHSNAGATIKYKISTVNPQATSPGALPSYSEHVQTPQKVALPFNRSSSSHRIEAFLYTKPPGLKSIQSTLETTSAASDNPFDNSHQLPNGGKWTYESPLSRWFLTKSLYLQDQVKQPLKSSNVKLKELNILSRVSKNKIQSPYPDETSPILSDTDRLSESKTSYASSQLDPLVYRSSSLKYTERKKPTGLTNIRAVSSAQRSPAGPHPQPSAAEVDIKPQFIRKTNSESSSFLDSSRVKNACTYKSSAPPNDRKKRRNNKKLRIHLDQIARKPLPLTPKPLLSGKKSGSLLKHAFMHKPLAGASDGLAQDEVYIVIRNYSPRLMDEITVQVGEHVKLLAKHTDGWCLVEKCDSSGNPLCEASSDQQEIGGEFYLNESRGIIPGVCLNKVTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKI9</accession>
    <name>C5DKI9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0F05082p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0F05082g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR23990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554427.1">
        <property type="nucleotide sequence ID" value="XM_002554381.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKI9"/>
    <dbReference type="FunCoup" id="C5DKI9">
        <property type="interactions" value="261"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKI9"/>
    <dbReference type="GeneID" id="8292625"/>
    <dbReference type="KEGG" id="lth:KLTH0F05082g"/>
    <dbReference type="eggNOG" id="ENOG502QSDX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_285457_0_0_1"/>
    <dbReference type="InParanoid" id="C5DKI9"/>
    <dbReference type="OMA" id="SSPECEY"/>
    <dbReference type="OrthoDB" id="299997at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="366"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="17"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="48"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="372"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR23990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1082" mass="121381" checksum="B350F23AF4B1B5A5" modified="2009-07-28" version="1">MQATTPISETRLKKVHTPPSRGSSSPSSLRSTKKKSGGVMNRVNLKFRALSSSSLPSPSRLAPEERPRIVHASSQRVPTENDHYFWPSNLPAAKPMTNTRKRRNTPAPLTLSQITPHATPQKQHGIKDATPKLESPMDFRHSTNSSTDNASWVTASTFNPRRRLNFELQGNFESPLDDSLTIERSSRSPWEDLYPDSESPTYASQLRSNMSKLSGPKNYTSNKCSICSEDLMFTLSGEKLIELSCNHQCHFNCYLVTFEALLPKSSFPQCRICTTASKPKSEDVMSEITAKILTSEAQGNDKTFMEQGGKQQRAIFERSDGNDVRPQTIFPITQNTPKENIYLQDLRTPREQLIKSAQVSCEGFKDHFSTQSTPRSKFSKFSSSDSFRTPSSTSQSEWHRSDFELDRPHVNIIPQLSKFSIKENSRQAIAQYVMSTYTPKHPAIAKGVEDYKNLIRKEVQSHVQSGFDYKPEMGALETFDILCYSEDEEVWVNVNAFLFSECLVFVQEDNLVGHILKNQISEMHQLDSHTLILNLKSKSLPEIFLCCPDDPSITRKWTYYLRQVYLTRGGEHVPLSQLTSNNWNFLPQKLIKKVRIPSETGFEDNDLSGTFALQEKTKLKLILCVSVVNCHPELHTNNEHLLSLKAKIKQTVDSLSSQDLLGLVLVGRDGCGNVGAFGTFFGMVGKDWDGWEDIIESLEVYDNNAVFANDSWELNAMLQTSRKLMCTVEQDESFLQQLVIIGNDYPGNMPRTIEDDFQVARLQSNVSLSLQKFRFSVHQYFTCNSRLLLSDIIEGYFYNVKCEGLNTLEEIRPDSLVQQLRKKAAKSFAVTLGTLDPNLAQFHAVERNGQLCKLPGLCTEVTMEIGALAPGEARNLIFEIEIDVQNLKHLLKNTSASNQPALLHYNATWHNGSRRLVFSGCSLGCEFKLGNVSTALPPTDALRDPPQSGHDANDECEILEVMLAPPLSASRDSLFATRHVELIAIETLRSALAQFPYHTHVLLNQLVPMIFCISRSCVCNDASYHTRVSTVKPLPQLVEQLCADIQEIAARSVSGNGADRALSRWEMARMTAALACGTYGAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>C5DKS3</accession>
    <name>C5DKS3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0F07084p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0F07084g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554511.1">
        <property type="nucleotide sequence ID" value="XM_002554465.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DKS3"/>
    <dbReference type="FunCoup" id="C5DKS3">
        <property type="interactions" value="951"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DKS3"/>
    <dbReference type="GeneID" id="8292714"/>
    <dbReference type="KEGG" id="lth:KLTH0F07084g"/>
    <dbReference type="eggNOG" id="KOG2963">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026936_2_0_1"/>
    <dbReference type="InParanoid" id="C5DKS3"/>
    <dbReference type="OMA" id="KDYTVMT"/>
    <dbReference type="OrthoDB" id="10261452at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007109">
        <property type="entry name" value="Brix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045112">
        <property type="entry name" value="PPAN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12661">
        <property type="entry name" value="PETER PAN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12661:SF5">
        <property type="entry name" value="SUPPRESSOR OF SWI4 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04427">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00879">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50833">
        <property type="entry name" value="BRIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Brix" evidence="2">
        <location>
            <begin position="26"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="278"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="354"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="283"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="354"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="423"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR24074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="436" mass="48888" checksum="F3EAF616930218A5" modified="2009-07-28" version="1">MAKRRQKTRTHVKPDAEEQKKVPKSMVIRVGQTSMANHSLNQLVKDFRQIMQPHTAIKLKERKSNKLKDFVVMCGPLGVSHLFIFTQSEKTGNVSLKVARTPHGPTVTYQVMDYSLGKDIKRFMKRPKALGKEDSLSPPLLVLNGFGIKPVEGDSESVEKANVEKVVISMFQNIFPPLNPGKTQLNSIKRVFMINKDPETGEISMRHYAIDIREVDISRNLKRLYKSKSKLNKAVPNLHKKDDIASLILDHDVGAYTSESEVEDDSIVKVMEKNGKSLKNAKSEASNNAQGSHQDAAEASAPRKKAIKLTEVGPRLTLKLVKIEEGICSGKVLHHSYVKKTSAEIRALEKKHAQRMKLKEERRKEQEANLARKKEAKDAKKQRKLERRKQREQAGEPAEGQGSDADADSSDSSSSDEGAYSDVPEDLDSDLYSDIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLA9</accession>
    <name>C5DLA9_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Autophagy-related protein 14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0F11484g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Preautophagosomal structure membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ATG14 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554697.1">
        <property type="nucleotide sequence ID" value="XM_002554651.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLA9"/>
    <dbReference type="FunCoup" id="C5DLA9">
        <property type="interactions" value="73"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLA9"/>
    <dbReference type="GeneID" id="8292910"/>
    <dbReference type="KEGG" id="lth:KLTH0F11484g"/>
    <dbReference type="eggNOG" id="ENOG502RY86">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069448_0_0_1"/>
    <dbReference type="InParanoid" id="C5DLA9"/>
    <dbReference type="OMA" id="MYCSHCI"/>
    <dbReference type="OrthoDB" id="4068791at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023261">
        <property type="entry name" value="Autophagy-related_protein_14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018791">
        <property type="entry name" value="UV_resistance/autophagy_Atg14"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10186">
        <property type="entry name" value="ATG14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02030">
        <property type="entry name" value="AUTOPHGYRP14"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0072">Autophagy</keyword>
    <keyword evidence="9 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0926">Vacuole</keyword>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="76"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR24260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="324" mass="37609" checksum="A2F81B81F4AC3408" modified="2009-07-28" version="1">MALKCCTCHRQSKVMYCSHCINTSPRLLLRYKMELYQVTAAREELRAQVDEILRDAMNGDEQPSDVLGKHLAMLRKTHLKRRTNKIRSKLQQLQEVVQVKRERVRTLKAQMNQPNPRVAHSVETDEKIAQFRSSQQKLTMLQRVLESSKALKFQALAHLFLIRQREHPEFPFTISFQPIVNVQNLCHLPQNVVECSLRSMWDFLLLAGRVLLIELPLQKPAKDVLDSLTGIVLNLLVFLQSLELVPQKFCGDRGYLRSLLRNYDVDRLFYYMVMNRDIELLDTGELHSAVNYDVCHAELQAMFQGSSELSVLNKSIDDKWFLVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLE0</accession>
    <name>C5DLE0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">KLTH0F12254p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">KLTH0F12254g</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endomembrane system</location>
            <topology evidence="8">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 9">Membrane</location>
            <topology evidence="1 9">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928170">
        <property type="protein sequence ID" value="CAR24291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002554728.1">
        <property type="nucleotide sequence ID" value="XM_002554682.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLE0"/>
    <dbReference type="FunCoup" id="C5DLE0">
        <property type="interactions" value="196"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLE0"/>
    <dbReference type="GeneID" id="8292945"/>
    <dbReference type="KEGG" id="lth:KLTH0F12254g"/>
    <dbReference type="eggNOG" id="KOG1693">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066963_4_2_1"/>
    <dbReference type="InParanoid" id="C5DLE0"/>
    <dbReference type="OMA" id="NAEDCFY"/>
    <dbReference type="OrthoDB" id="1929172at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome F"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015720">
        <property type="entry name" value="Emp24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036598">
        <property type="entry name" value="GOLD_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
        <property type="entry name" value="TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101576">
        <property type="entry name" value="Supernatant protein factor (SPF), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5002950419" evidence="11">
        <location>
            <begin position="23"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="176"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="12">
        <location>
            <begin position="39"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAR24291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="210" mass="23571" checksum="045F6FDEE5B3FA06" modified="2009-07-28" version="1">MLAQSIVSVFAALFLCITGVLANANSYSPVALMLPSFSKECLYYDLKSNDDSLVISYQVLTGGNFEIDFEITAPDGRAIATETHKKYSDFLLKSFGLGEYSFCFSNNYGTALKKVEVTLELEKENLGANAAPEDAIANNAIEEIERNLNKITKMMNYLRAREWRNMYTVKSTESRLVWMSMLTMGLTVGISVIQAAIVQFLFKDRQKNYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLG0</accession>
    <name>C5DLG0_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Phosphotransferase</fullName>
            <ecNumber evidence="12">2.7.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0G00440g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>D-fructose + ATP = D-fructose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16125"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37721"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:16126"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a D-hexose + ATP = a D-hexose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22740"/>
            <dbReference type="ChEBI" id="CHEBI:4194"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:229467"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:22741"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate metabolism; hexose metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the hexokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.-" evidence="12"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555058.1">
        <property type="nucleotide sequence ID" value="XM_002555012.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLG0"/>
    <dbReference type="FunCoup" id="C5DLG0">
        <property type="interactions" value="1010"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLG0"/>
    <dbReference type="GeneID" id="8293316"/>
    <dbReference type="KEGG" id="lth:KLTH0G00440g"/>
    <dbReference type="eggNOG" id="KOG1369">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014393_5_2_1"/>
    <dbReference type="InParanoid" id="C5DLG0"/>
    <dbReference type="OMA" id="ADCVQQF"/>
    <dbReference type="OrthoDB" id="419537at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00180"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005536">
        <property type="term" value="F:D-glucose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008865">
        <property type="term" value="F:fructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004340">
        <property type="term" value="F:glucokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019158">
        <property type="term" value="F:mannokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046323">
        <property type="term" value="P:D-glucose import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990539">
        <property type="term" value="P:fructose import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006000">
        <property type="term" value="P:fructose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001678">
        <property type="term" value="P:intracellular glucose homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006013">
        <property type="term" value="P:mannose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1250:FF:000001">
        <property type="entry name" value="Phosphotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000092">
        <property type="entry name" value="Phosphotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.367.20:FF:000004">
        <property type="entry name" value="Phosphotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.367.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001312">
        <property type="entry name" value="Hexokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019807">
        <property type="entry name" value="Hexokinase_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022673">
        <property type="entry name" value="Hexokinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022672">
        <property type="entry name" value="Hexokinase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19443">
        <property type="entry name" value="HEXOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19443:SF16">
        <property type="entry name" value="HEXOKINASE TYPE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00349">
        <property type="entry name" value="Hexokinase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03727">
        <property type="entry name" value="Hexokinase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00475">
        <property type="entry name" value="HEXOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00378">
        <property type="entry name" value="HEXOKINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51748">
        <property type="entry name" value="HEXOKINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 12" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="7 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Hexokinase N-terminal" evidence="13">
        <location>
            <begin position="27"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="Hexokinase C-terminal" evidence="14">
        <location>
            <begin position="226"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF03727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR24621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="485" mass="53818" checksum="DC02DC3FC5244827" modified="2009-07-28" version="1">MVHLGPKKPPMRKGSMIDVPKNLMEQIQSLEEQFTVSGEKLKEVTKHFVGELNKGLSKKGGNIPMIPGWVMEFPTGQETGDYLAIDLGGTNLRVVLVRLLGNHKFDTTQSKYKLPDHIRTSSSEELFSFIADSLKTFVDEEFPEGVTEPLPLGFTFSYPCSQDKVNEGFLQRWTKGFDIEGVEGEDVVPMLQEQLVKRKIPIDVVALINDTTGTLVASMYTDAETRMGCIFGTGVNGAYFDVVSDIEKLEGRLPSDITSDLPMAINCEYGSFDNELVVLPRTKYDIIVDKQSPRPGQQSFEKMTSGYYLGELLRLILLDLQQQGLIFEGQDLTKLQKPYVMDTSYPARIEEDPFENLEDTYDIFKNELGVETTGGERKLIRRACELIGTRASRMSMCGIGAVCTKRGYKTAHIAADGSVFNKYPEFKERAAAGLRDIFDWEPRQNIEEYPIVIVAAEDGSGAGAAIIAALTQKRLAAGKSVGVAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLH1</accession>
    <name>C5DLH1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glutamate--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Glutamyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0G00682g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>tRNA(Glu) + L-glutamate + ATP = L-glutamyl-tRNA(Glu) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9663"/>
            <dbReference type="Rhea" id="RHEA-COMP:9680"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.17"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.17" evidence="3"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555069.1">
        <property type="nucleotide sequence ID" value="XM_002555023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLH1"/>
    <dbReference type="FunCoup" id="C5DLH1">
        <property type="interactions" value="910"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLH1"/>
    <dbReference type="GeneID" id="8293327"/>
    <dbReference type="KEGG" id="lth:KLTH0G00682g"/>
    <dbReference type="eggNOG" id="KOG1147">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001882_1_2_1"/>
    <dbReference type="InParanoid" id="C5DLH1"/>
    <dbReference type="OMA" id="FIHIPDG"/>
    <dbReference type="OrthoDB" id="10250478at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017102">
        <property type="term" value="C:methionyl glutamyl tRNA synthetase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004818">
        <property type="term" value="F:glutamate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990825">
        <property type="term" value="F:sequence-specific mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006424">
        <property type="term" value="P:glutamyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000070">
        <property type="entry name" value="Bifunctional glutamate/proline--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1160.10:FF:000001">
        <property type="entry name" value="Glutamine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.800.10:FF:000001">
        <property type="entry name" value="Glutamine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.240.10:FF:000004">
        <property type="entry name" value="Glutamyl-tRNA synthetase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000036">
        <property type="entry name" value="Putative glutamyl-tRNA synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1160.10">
        <property type="entry name" value="Glutamyl-trna Synthetase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.800.10">
        <property type="entry name" value="Glutamyl-tRNA Synthetase, Domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.240.10">
        <property type="entry name" value="Ribosomal Protein L25, Chain P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02076">
        <property type="entry name" value="Glu_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050132">
        <property type="entry name" value="Gln/Glu-tRNA_Ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004526">
        <property type="entry name" value="Glu-tRNA-synth_arc/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000924">
        <property type="entry name" value="Glu/Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020058">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020059">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_codon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020061">
        <property type="entry name" value="Glu_tRNA_lig_a-bdl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041103">
        <property type="entry name" value="GluRS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020056">
        <property type="entry name" value="Rbsml_bL25/Gln-tRNA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011035">
        <property type="entry name" value="Ribosomal_bL25/Gln-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049437">
        <property type="entry name" value="tRNA-synt_1c_C2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00463">
        <property type="entry name" value="gltX_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43097:SF5">
        <property type="entry name" value="GLUTAMATE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43097">
        <property type="entry name" value="GLUTAMINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18466">
        <property type="entry name" value="GluRS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00749">
        <property type="entry name" value="tRNA-synt_1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03950">
        <property type="entry name" value="tRNA-synt_1c_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20974">
        <property type="entry name" value="tRNA-synt_1c_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00987">
        <property type="entry name" value="TRNASYNTHGLU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50715">
        <property type="entry name" value="Ribosomal protein L25-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="8 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9 13" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST C-terminal" evidence="14">
        <location>
            <begin position="24"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR24632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="704" mass="80442" checksum="0FD58579422FCCF9" modified="2009-07-28" version="1">MAKLIIASKAPVVAYSELIAARLVNSAKTDAIEIEFVEDKKSSPATFEDSCDDVLKRIVQTYPEVFKHGFENCDEWAKLASEELTVKNFQKLSVSLAKLDACLNFRTFILGGTTFSAADIACWGSLRSNGMVGSIIKNKVYVNVSRWYTLLEQIPEFGSAHEFLVKTLQELKKASTSNKKKETHKANFEIDLQDAKVGEVVTRFPPEPSGYLHIGHAKAAILNQYFAQEYKGKLIIRFDDTNPSKEKEEFQDSILEDLELLGIKGDRVTYSSDYFQEMYDLCVKMIEEGNAYCDDTPTDKMREERMDGLASARRDRSVEENLRIFTQEMKNGTEEGLKNCVRAKIDYKALNKTLRDPVIYRCNLTPHHRTGSTWKIYPTYDFCVPIVDALEGVTHALRTIEYRDRNAQYQWMLDALHLRKVHIWDFARVNFVRTLLSKRKLQWMVDKDLVSNWDDPRFPTVRGVRRRGMTVEGLRNFVLSQGPSRNVINLEWNLIWAFNKKVIDPVAPRHTAIVKPVKFHVEGAPQTPDVQIKLKHKKNPAVGEKKVIYYADILIDQDDADAVTEGEELTLMDWGNAIVTKKNADGSLTGKLHLEGDFKKTKLKLTWLADTDDKVDVDLVDFDHLITKDKLEEGEDFADYLTPQTEFHVSAVADLNVKDMKKGDIIQFERKGYYRLDSFATADKPYVFFAIPDGKSVNKYGAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLM8</accession>
    <name>C5DLM8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 18">CDP-diacylglycerol--serine O-phosphatidyltransferase</fullName>
            <ecNumber evidence="5 18">2.7.8.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16 18">Phosphatidylserine synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="ordered locus">KLTH0G01936g</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 18">
            <text>a CDP-1,2-diacyl-sn-glycerol + L-serine = a 1,2-diacyl-sn-glycero-3-phospho-L-serine + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16913"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
            <dbReference type="ChEBI" id="CHEBI:58332"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="2.7.8.8"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="17 18">Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from CDP-diacylglycerol: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 18 19">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.8" evidence="5 18"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555126.1">
        <property type="nucleotide sequence ID" value="XM_002555080.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLM8"/>
    <dbReference type="FunCoup" id="C5DLM8">
        <property type="interactions" value="115"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLM8"/>
    <dbReference type="GeneID" id="8293384"/>
    <dbReference type="KEGG" id="lth:KLTH0G01936g"/>
    <dbReference type="eggNOG" id="ENOG502QPJG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049944_5_0_1"/>
    <dbReference type="InParanoid" id="C5DLM8"/>
    <dbReference type="OMA" id="HGCGMIS"/>
    <dbReference type="OrthoDB" id="448573at2759"/>
    <dbReference type="UniPathway" id="UPA00558">
        <property type="reaction ID" value="UER00615"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003882">
        <property type="term" value="F:CDP-diacylglycerol-serine O-phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006659">
        <property type="term" value="P:phosphatidylserine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1760:FF:000022">
        <property type="entry name" value="CDP-diacylglycerol--serine O-phosphatidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050324">
        <property type="entry name" value="CDP-alcohol_PTase-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004533">
        <property type="entry name" value="CDP-diaglyc--ser_O-PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016271">
        <property type="entry name" value="CDP-diaglyc--ser_O-PTrfase_fun"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048254">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00473">
        <property type="entry name" value="pssA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14269">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--GLYCEROL-3-PHOSPHATE 3-PHOSPHATIDYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14269:SF61">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--SERINE O-PHOSPHATIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000852">
        <property type="entry name" value="Phosphatidylserine_synth_fun"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 18" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 18" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="12 18" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13 18 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="14 18" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="15 18" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 18 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="107"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="145"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="31"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00032361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00060701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRNR" id="PIRNR000852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="CAR24689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="270" mass="29723" checksum="A82AD846A2D5FB11" modified="2009-07-28" version="1">MSKRGEQVPREEATQDLGGAVSSDAEDDAFTASRTPSRRASSIFSLDNPPLEPPNESDIRKFTSDEYHFNMIRNLHLADLITMMNGFSGFYSIVSCLRFTLTGKPHYVQRAHFFILLGMFFDFFDGKVARLRNRSSLMGQELDSLADLVSFGVAPASIAFAIGFQTTLDVLVLSFFMLCGLTRLARFNVTVAQLPKDVSGKSKFFEGLPIPTSLALVGAMAYLVSKGLILEKLPFGLFAAGEWYELHPAVLLFGIQGCGMISKSLKIPKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLR8</accession>
    <name>C5DLR8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA-directed RNA polymerase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0G02926g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">DNA-dependent RNA polymerase which catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555166.1">
        <property type="nucleotide sequence ID" value="XM_002555120.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLR8"/>
    <dbReference type="FunCoup" id="C5DLR8">
        <property type="interactions" value="1153"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLR8"/>
    <dbReference type="GeneID" id="8293429"/>
    <dbReference type="KEGG" id="lth:KLTH0G02926g"/>
    <dbReference type="eggNOG" id="KOG3298">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085878_2_0_1"/>
    <dbReference type="InParanoid" id="C5DLR8"/>
    <dbReference type="OMA" id="TMRQPGL"/>
    <dbReference type="OrthoDB" id="1162399at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060213">
        <property type="term" value="P:positive regulation of nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045948">
        <property type="term" value="P:positive regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04329">
        <property type="entry name" value="RNAP_II_Rpb7_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04462">
        <property type="entry name" value="S1_RNAPII_Rpb7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000043">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.120:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.120">
        <property type="entry name" value="RNA polymerase Rpb7-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036898">
        <property type="entry name" value="RNA_pol_Rpb7-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045113">
        <property type="entry name" value="Rpb7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005576">
        <property type="entry name" value="Rpb7-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709:SF4">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II, III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03876">
        <property type="entry name" value="SHS2_Rpb7-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88798">
        <property type="entry name" value="N-terminal, heterodimerisation domain of RBP7 (RpoE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="S1 motif" evidence="7">
        <location>
            <begin position="84"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU369086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR24729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="171" mass="18950" checksum="51FEA58F9331D3B9" modified="2009-07-28" version="1">MFFFKDLSLNITLHPSFFGPQMKQYLRTKLLQEVEGTCTGKFGYILCVLDCENVEIERGRILPGDGSAEFTVKYRAVVWKPFKGEVVDGVVSSCTNLGFEVDVGPLSVFVSPFLMPEDLSYNSGSNPPSYQSSEQIITKGSRVRLKIVGARSEVNSIYAIGSIKEDYLGVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DLT4</accession>
    <name>C5DLT4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Carnitine O-acetyltransferase, mitochondrial</fullName>
            <ecNumber evidence="16">2.3.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ordered locus">KLTH0G03300g</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Carnitine acetylase is specific for short chain fatty acids. Carnitine acetylase seems to affect the flux through the pyruvate dehydrogenase complex. It may be involved as well in the transport of acetyl-CoA into mitochondria.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>(R)-carnitine + acetyl-CoA = O-acetyl-(R)-carnitine + CoA</text>
            <dbReference type="Rhea" id="RHEA:21136"/>
            <dbReference type="ChEBI" id="CHEBI:16347"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57589"/>
            <dbReference type="EC" id="2.3.1.7"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Matrix side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 19">Belongs to the carnitine/choline acetyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.7" evidence="16"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555182.1">
        <property type="nucleotide sequence ID" value="XM_002555136.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DLT4"/>
    <dbReference type="FunCoup" id="C5DLT4">
        <property type="interactions" value="223"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DLT4"/>
    <dbReference type="GeneID" id="8293446"/>
    <dbReference type="KEGG" id="lth:KLTH0G03300g"/>
    <dbReference type="eggNOG" id="KOG3717">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013513_5_1_1"/>
    <dbReference type="InParanoid" id="C5DLT4"/>
    <dbReference type="OMA" id="KMDGTPT"/>
    <dbReference type="OrthoDB" id="240216at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004092">
        <property type="term" value="F:carnitine O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009437">
        <property type="term" value="P:carnitine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.70:FF:000007">
        <property type="entry name" value="Carnitine O-acetyltransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.70">
        <property type="entry name" value="Choline/Carnitine o-acyltransferase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000542">
        <property type="entry name" value="Carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039551">
        <property type="entry name" value="Cho/carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042231">
        <property type="entry name" value="Cho/carn_acyl_trans_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589:SF103">
        <property type="entry name" value="CARNITINE O-ACETYL-TRANSFERASE, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589">
        <property type="entry name" value="CARNITINE O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00755">
        <property type="entry name" value="Carn_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00439">
        <property type="entry name" value="ACYLTRANSF_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00440">
        <property type="entry name" value="ACYLTRANSF_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 19" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Choline/carnitine acyltransferase" evidence="20">
        <location>
            <begin position="58"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="18">
        <location>
            <position position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00052702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00053195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00066910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00073438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR600542-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU003801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="CAR24745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="649" mass="74265" checksum="A137FE1AAD289FF5" modified="2009-07-28" version="1">MREISRVVRRMVSTLQRFSFETRNGESYSALRPNSYYQKKRSTFKGTTFSHQNQLPSLPVPVLEETLTKYLESVKPFCRDAEQFEEQERLCQDLVQKEGAKLQERLVRYSEKSRNWLSEFWDNQAYLEYNDPVVPYVSYFYAHKPLPTSHAKIQTDPLIKATAIIVSVLKFLEAVKDEALPSEHSGNTLLCMNSFQMMFNNSRVPGDGRDSNVFYSLYENNFIVVAFKGNFYKVLTHNASGDVLGPADIWQQLYKITANLDGQVRDNNKVGIGSLTSLPRDEWLRAHKELVTNPLSAQSMEAIYKSSFMVCLDLDTKPITLEEKSRYAWHGDGINRFYDKPLQFFVAGNGNSGFLAEHSKMDGTPTLHLNDYVCKQMHSLNVAEFLGQINCSVAQNENKPQYLPFFVTPATRSHIEAAQVGFHKRIGEHHLKVWHYNKYGKNFMKNVGFSPDAFLQQVIQLAIYKYFGKQLPTYEAASTRRFYKGRTETGRSVSTDSAKFVADWENPDVADSQKVASLRSSAKAHSTYLKMASAGHGVDRHFFGLKNMIQPGENMPELFKNPLFIYSSTWLVSTSQLSSEHFDGYGWSQVNDNGFGLAYMLNKDWMHINIVNKPNASGLSVSRLHYYLTQAADEMFELLDSDRKHHSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>C5DM40</accession>
    <name>C5DM40_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0G05764p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0G05764g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555288.1">
        <property type="nucleotide sequence ID" value="XM_002555242.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DM40"/>
    <dbReference type="GeneID" id="8293555"/>
    <dbReference type="KEGG" id="lth:KLTH0G05764g"/>
    <dbReference type="HOGENOM" id="CLU_1468417_0_0_1"/>
    <dbReference type="InParanoid" id="C5DM40"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR24851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="184" mass="20470" checksum="957486884B288AA5" modified="2009-07-28" version="1">MRAECRRKDVTSVEPSTKQAVARAPDPRRIWACGSGAGRTAGRPSECMRASDGAPVSRRRRARGARRSSWMVTDVHVCITCAQEALDESPSAFLLSNRRYNISTARASEGPLRSTLARARCFPKANRGAVDFVYGDSDYKGAVTNVFVYFRPFAPGTHVFKIASQLVFQKMIYKICGFLRKLWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMA8</accession>
    <name>C5DMA8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Nucleoporin NSP1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Nuclear pore protein NSP1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Nucleoskeletal-like protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">KLTH0G07326g</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Nucleoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the nucleoporin NSP1/NUP62 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555356.1">
        <property type="nucleotide sequence ID" value="XM_002555310.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMA8"/>
    <dbReference type="STRING" id="559295.C5DMA8"/>
    <dbReference type="GeneID" id="8293626"/>
    <dbReference type="KEGG" id="lth:KLTH0G07326g"/>
    <dbReference type="eggNOG" id="KOG2196">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4719">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020542_0_0_1"/>
    <dbReference type="InParanoid" id="C5DMA8"/>
    <dbReference type="OMA" id="EMMSKQV"/>
    <dbReference type="OrthoDB" id="344345at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044613">
        <property type="term" value="C:nuclear pore central transport channel"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006405">
        <property type="term" value="P:RNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000040">
        <property type="entry name" value="Nuclear pore glycoprotein p62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026010">
        <property type="entry name" value="NSP1/NUP62"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007758">
        <property type="entry name" value="Nucleoporin_NSP1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12084:SF0">
        <property type="entry name" value="NUCLEAR PORE GLYCOPROTEIN P62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12084">
        <property type="entry name" value="NUCLEAR PORE GLYCOPROTEIN P62-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05064">
        <property type="entry name" value="Nsp1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="9" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Nucleoporin NSP1-like C-terminal" evidence="15">
        <location>
            <begin position="698"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="31"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="45"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="89"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="118"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="161"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="197"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="216"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="277"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="492"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="587"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="597"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="615"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="638"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="674"/>
            <end position="685"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00068864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00078941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00081079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF05064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAR24919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="897" mass="92420" checksum="A72DF1EDE19BE309" modified="2009-07-28" version="1">MSFQFNLNSNSSANFGGNSPFGSASAPTKDQNSQSSGFSGFSSNPSTNAQQTSGNTPGFGASQGQQGSLFGKPLGGTTPAFGSSSAPKPFGSLGGSSPFGAQPATTTPANENDKKESQSTGFNLGGNTASANNAKPTAPFSFGTTPAATGKDDKPLFGGASSASPFQFGAASNQGTSAPAQSSTNKPTLNFGSSSNSKPAFSFGAASASDAKSGLGDTTKTLESAAQTSSAGSKSPFTFGASTAKSTETNASSGPGTSTVEKKDDAKPAFSFGGTSGTNTAAPKSSPSLNFGGSAANKKEESKPAFSFGTAASEKKEEPMPAFSFGGTSSTTKEDSKPAFSFGGAPSDKKEDSKPAFSFGEAANNKKEDPKPAFSFGATASDKKENPKPALSFGGATADKTEASKPELSLGGTTTNKNEVSKPAFSLGTASSDIKEDPKPAFSFGTANNDKKEEAKPAFKLGGLGEKKDEPKSTFAFGAPGEKSADKPVSSKPETNDGNASSKPSLGFLAGSENKSGGKPAFSFGKSAESTNASDKRVGFGNSSEQGNKLGAGTSEASKGSFNFGGLGAQQQKSDANKGGSLSFGAKDPKQDKENKSFGFSNSSGTGSDSKPSATGFSFGQNKESATSSKPSGFSFGAKDKDKEEKDAPKTTLSFRKDTDGSSTSKPSNFGFGSKKEAEKKDGTQREAAPSSTGTEAQKPIEIQPVSLDNKTLDDLVTKWTSQLGGSVDHFATYADKVKEWDQVLMLGGEQIGQLYSDALVAEQTQNRVDQSLQYIERQQSELETFLDNYEKKAEGLLSGVLSNSNGSTGNINDQKRQQAYQTAEILDDNLNSLSMNLSALISEINQVSDTFNKATNMSVTNEDENTQLIKLLNSHLDALKSLDNSSDLLEQKLTKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMG3</accession>
    <name>C5DMG3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0G08668p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0G08668g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555411.1">
        <property type="nucleotide sequence ID" value="XM_002555365.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMG3"/>
    <dbReference type="FunCoup" id="C5DMG3">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMG3"/>
    <dbReference type="GeneID" id="8293686"/>
    <dbReference type="KEGG" id="lth:KLTH0G08668g"/>
    <dbReference type="eggNOG" id="ENOG502QTWB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043059_2_0_1"/>
    <dbReference type="InParanoid" id="C5DMG3"/>
    <dbReference type="OMA" id="SLMIRWI"/>
    <dbReference type="OrthoDB" id="2139606at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021100">
        <property type="entry name" value="N-glycosylation_EOS1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28147">
        <property type="entry name" value="N-GLYCOSYLATION PROTEIN EOS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28147:SF1">
        <property type="entry name" value="N-GLYCOSYLATION PROTEIN EOS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12326">
        <property type="entry name" value="EOS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02070">
        <property type="entry name" value="NGLYCOSEOS1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="153"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="183"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="215"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="306"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="251"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="251"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR24974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="352" mass="39831" checksum="FCD739DC8A3B2E56" modified="2009-07-28" version="1">MEDTLERNAEQLPVPLESRSSQIHIHHHHHYHRRTASEGGSQGKREAGSSKKNTYRSIKSLTLNNLDARQHFLIAVCRDVSLLPPLLSLAHSLKKAWSLWYRKDMLYATDNNSGSLTMSFTKLWQTYRTLRTQASSGGTDLSSQLITARSSEYILCSLWCVVSAYLTYAILDSLMVRWIVKYSTIAAILRMFSMSLLIITVEMLLLSSLSPKGDYYLHTWILISCVLTGAYIWQSFLTSDLSYVEQNPISAGSTSAYGRRSSSSHSLSALSDPTNASQEDLAQTASRKKKNAHTVFNFAKNKQIHFYNITVFCVVPVGFASFITMIGLLRNLVIQRLDVEQLERIIREVYND</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMI2</accession>
    <name>C5DMI2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MMS19 nucleotide excision repair protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0G09152g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Key component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into apoproteins specifically involved in DNA metabolism and genomic integrity. In the CIA complex, MMS19 acts as an adapter between early-acting CIA components and a subset of cellular target iron-sulfur proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the MET18/MMS19 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR24993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555430.1">
        <property type="nucleotide sequence ID" value="XM_002555384.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMI2"/>
    <dbReference type="FunCoup" id="C5DMI2">
        <property type="interactions" value="1029"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMI2"/>
    <dbReference type="GeneID" id="8293707"/>
    <dbReference type="KEGG" id="lth:KLTH0G09152g"/>
    <dbReference type="eggNOG" id="KOG1967">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005943_1_0_1"/>
    <dbReference type="InParanoid" id="C5DMI2"/>
    <dbReference type="OMA" id="FSFMPEF"/>
    <dbReference type="OrthoDB" id="342900at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039920">
        <property type="entry name" value="MMS19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024687">
        <property type="entry name" value="MMS19_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029240">
        <property type="entry name" value="MMS19_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12891">
        <property type="entry name" value="DNA REPAIR/TRANSCRIPTION PROTEIN MET18/MMS19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12891:SF0">
        <property type="entry name" value="MMS19 NUCLEOTIDE EXCISION REPAIR PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12460">
        <property type="entry name" value="MMS19_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14500">
        <property type="entry name" value="MMS19_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="MMS19 N-terminal" evidence="7">
        <location>
            <begin position="52"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="MMS19 C-terminal" evidence="6">
        <location>
            <begin position="571"/>
            <end position="967"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU367072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR24993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1019" mass="115106" checksum="D18CF36B45367F3A" modified="2009-07-28" version="1">MSHSYSSQNLRADVVSIIANVESDEAKALELSKKVALKVSEGQLKLVELVICLREYITSPEQIERQHALHCLSTILGELPKHTLLKNDVSVILDFYLGKFDDIYCMKETLYGLSHIVNMKCFYSNQVSSVLKPLKDDYQSTQYLAATRYYAFLVLQHLLEKFRESMSTNLPLNNLFIETFLSIAGGEKDPRNLLISFHLNSAISSALENISQYKEDLFDTLFCYFPITFKPPKNDPYRITNIDLKVALRSAISASPYFEEDAFGNLIDKMTASSPSVKNDTLLTMKACIERFGGEACIKNWVPIWNALKFEIMNNVDADESVTESNEPEFNNYEESLRIITSISAQLLNLKEGAFDKFQSHIFDELEPNFKYEKDLKQSSAILAAVARTNVTSFNKVMDDVLKLFFDNVSDLDVNKLKLLILNLSFFFDAYIEVFSPYSNKAQNKAVPDNKLAQYKDQILMILSKSLKGSSKSEVSLRTLSVVQLTKLVKMPGFLSGEEVSLIVQYLTETILTDDNKNVYFACLEGLKVTSDVSENSVVEISLNQMLKHLEKEGTHTVVLGEEEAVPVERILKVILDFTTSRHKLVVESIIGISNIICHFSKYVEEKEYCFMLVSCLYSLFDNNQEVLTNDVASHLKKSIETELLASSKNVAIYSDDHNLHLISAVFYFLNVNAPQANHQIELDRFLVLFTEDTKVLKSPARSVIIFAKLLSAFDKACKIPGGLLLNESIRLLKVQHEKCSEFEKLAYLELLAVLSNKWCTEDNVNANLDISNTALIDLEIEAWCTKGLAMKNSKLAETYTDKFVKLLGDADIGPASAKLFGVFVMDIPTLDRIKGISWNNNVRLLYKQKLFSDVAPKLVSGYSATSDMSLKANYLLALSLLLKNTPNKITINYIPNLLSLLLQAVQLNNSDVRVSALTTIRGTVNQASQLITEHIHSLIPLLMRLTNPDKHNAYLVRLTALEILRDFTDHVPLNYLLPYKDDIIKGLAVALDDKKRRVRKTCIDTRQAYFELGQVPFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMJ0</accession>
    <name>C5DMJ0_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KLTH0G09350p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">KLTH0G09350g</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. CAR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555438.1">
        <property type="nucleotide sequence ID" value="XM_002555392.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMJ0"/>
    <dbReference type="FunCoup" id="C5DMJ0">
        <property type="interactions" value="86"/>
    </dbReference>
    <dbReference type="GeneID" id="8293716"/>
    <dbReference type="KEGG" id="lth:KLTH0G09350g"/>
    <dbReference type="eggNOG" id="KOG0255">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008455_8_4_1"/>
    <dbReference type="InParanoid" id="C5DMJ0"/>
    <dbReference type="OMA" id="NWNYRRR"/>
    <dbReference type="OrthoDB" id="440553at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140115">
        <property type="term" value="P:export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042908">
        <property type="term" value="P:xenobiotic transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17323">
        <property type="entry name" value="MFS_Tpo1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF51">
        <property type="entry name" value="QUINIDINE RESISTANCE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="77"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="112"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="170"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="200"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="284"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="324"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="396"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="421"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="459"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="483"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="46"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="529"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="529"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="548"/>
            <end position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAR25001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="563" mass="60731" checksum="2991A4DFFF9551AB" modified="2009-07-28" version="1">MGENYINQGKQADPVPDLPHMKPNMPDHDQGAVPYSRFGSNEKLVLVAICAASGIFSTIAGPIYYPALTTIENQFHISTELVNVSVVLYFLFQGLAPTLMGGLADSFGRRPIVMWSIALYLAACIGIACSRTYAQILVLRCLQSAGISPVIAVNSGIMGDVTTRAQRGGYVGLTAGFQVLGSAFGALIGGGIVSTWGWRAIFWFLAIGSGCSLVCSSLLLPETKRSVVGNGSITPPAIWSKAPILALPQIRKKLHLDSPDYETLAPKERLNLLAPLDVFRRPEIALLLLVTGLQFSLWVCHLTALSSLLEKDYHLKVAEVGLSYLPSGICTLISVVGAGRVLNWNYRRRLSKHNLFIEEATQRLLAENDNDEKRVDKILSTDISYTFNIFRARLEIMFVPLLLSGGGFMGFGWCLDAHTPLVAVLVFSGFASLFSNCVIACATTLVVDLYPQKSSTATGCVNFSRCVLAAILVAALNKMLTSLSVGGTFTLLAGLTILSTVTLIIPIKYGPGMAHKRAVRDRTAADEKAEAAVKEVDQQRQSSESEDLSSLNTISQPRSRAGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMQ6</accession>
    <name>C5DMQ6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0G10824p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0G10824g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SAM50/omp85 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555504.1">
        <property type="nucleotide sequence ID" value="XM_002555458.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMQ6"/>
    <dbReference type="FunCoup" id="C5DMQ6">
        <property type="interactions" value="172"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMQ6"/>
    <dbReference type="GeneID" id="8293782"/>
    <dbReference type="KEGG" id="lth:KLTH0G10824g"/>
    <dbReference type="eggNOG" id="KOG2602">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014798_3_1_1"/>
    <dbReference type="InParanoid" id="C5DMQ6"/>
    <dbReference type="OMA" id="KHPVARF"/>
    <dbReference type="OrthoDB" id="1724197at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045040">
        <property type="term" value="P:protein insertion into mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.50">
        <property type="entry name" value="membrane protein fhac: a member of the omp85/tpsb transporter family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000184">
        <property type="entry name" value="Bac_surfAg_D15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039910">
        <property type="entry name" value="D15-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12815:SF18">
        <property type="entry name" value="SORTING AND ASSEMBLY MACHINERY COMPONENT 50 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12815">
        <property type="entry name" value="SORTING AND ASSEMBLY MACHINERY SAMM50 PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01103">
        <property type="entry name" value="Omp85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1134">Transmembrane beta strand</keyword>
    <feature type="domain" description="Bacterial surface antigen (D15)" evidence="6">
        <location>
            <begin position="184"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR25067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="495" mass="55276" checksum="C3B39686CF5B712B" modified="2009-07-28" version="1">MNGPASDNEELETRILEASQRSSKLEEIDLEHQKVLQNQQLLYAKDLFSNKAHTPVHVSSVVVNNAECVRPSVLQNYLDQTITRATTFEHLCELSDILNMKLISNGLVENCTQTLDSRGVLHYELQDSDPKPSYATPHSTGHTVSVVDIVPIVNLQPLKKISAKTGTNIGNGEGDGYLQLQWRNALGGGEKFTFDATKGTKTHSSYLFDYSQPLSPWWLWDCSVYKNSRSLGNMELLMRGTRASVRSAFEKHKHLNYELGVESLWRSTQATSTHSSDSLLLLAGDEVKNSLSHSVFWDTRDRPIFPLAGSFYKLTNELSPSKYWKCFFEASKVKSWRKNDFFTASFTLKGGYINNFSASKPLHISDKFHNGGSNDVRSFQLMGLGPKDLHDSLGGDAFVSYGVSLFSRLPFKRWSDSNFRLHAFFNGARLINTNGDQLKNCISSLAREHSTSTGIGLVLGHPVARFELNFGIPLTAHTSDSARKGFQYGIGLSFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2023-06-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMS1</accession>
    <name>C5DMS1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0G11176p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0G11176g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555519.1">
        <property type="nucleotide sequence ID" value="XM_002555473.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMS1"/>
    <dbReference type="GeneID" id="8293798"/>
    <dbReference type="KEGG" id="lth:KLTH0G11176g"/>
    <dbReference type="HOGENOM" id="CLU_1421654_0_0_1"/>
    <dbReference type="InParanoid" id="C5DMS1"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR25082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="191" mass="21386" checksum="4F3F7471521EAB31" modified="2009-07-28" version="1">MQAAATITKSGVKHFRKAPRNISWVIRAAYFGFGKPRLRKNVFPYRQVTPRGGVRRAPVTPETSGGPEQSRCCRRNTAFILRLGTLGGLEHRVRAYDRILIQPLLPTISPWTMACSALGTAARGHATARWPRKASPVLLPRISTHFQGACASPRSRIYVCCRARSQPQRDFTPYGSLARKYGLEPRLRALF</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMU3</accession>
    <name>C5DMU3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0G11660p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0G11660g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555541.1">
        <property type="nucleotide sequence ID" value="XM_002555495.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMU3"/>
    <dbReference type="FunCoup" id="C5DMU3">
        <property type="interactions" value="57"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMU3"/>
    <dbReference type="GeneID" id="8293820"/>
    <dbReference type="KEGG" id="lth:KLTH0G11660g"/>
    <dbReference type="eggNOG" id="ENOG502QUD1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_390400_0_0_1"/>
    <dbReference type="InParanoid" id="C5DMU3"/>
    <dbReference type="OMA" id="LWFELDE"/>
    <dbReference type="OrthoDB" id="5392646at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048401">
        <property type="entry name" value="SLS1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032741">
        <property type="entry name" value="Sls1_KH-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048748">
        <property type="entry name" value="SLS1_KH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048400">
        <property type="entry name" value="SLS1_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14611">
        <property type="entry name" value="KH_SLS1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20777">
        <property type="entry name" value="KH_SLS1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20778">
        <property type="entry name" value="SLS1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20776">
        <property type="entry name" value="SLS1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SLS1 N-terminal" evidence="2">
        <location>
            <begin position="133"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="SLS1 first KH" evidence="1">
        <location>
            <begin position="227"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="SLS1 second KH" evidence="3">
        <location>
            <begin position="309"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="SLS1 C-terminal" evidence="4">
        <location>
            <begin position="389"/>
            <end position="696"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF20778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR25104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="698" mass="79684" checksum="A5B6B6EA0AF60159" modified="2009-07-28" version="1">MFSRCTQAVIRCQRKGFDGCSRFPKLPLGLNRSNSTTTKVLVSDKALHSTRGKKLKPIKAQIVVLNPREAGLFKRKRTPAYFNGNERPRERLSVLEGVEFDSGGTSNNRKQQSSGDILQEIESQRRKLLVFKSSVPNEQVIKSIHDLRPSDDTRVMSTKRYTQLKALLEMAYTLPQLKAYTRQYYDQRVPKSVTKKSIIPSILHKLWNCEVDASMSDEQDLVVERILDSQSRDIYLLLLTNNGKILHNFARLGATVAVALGENKIIVRATSSLVKYVEVSLRKILDNVQREVLPIQSIIDSHSASNSPPISPEDLVNLVQKETATFFEKLLEDDTNSYVVSGFGGKRLAKAEILLLWALDYNPQKTELVRFLGERGDCDYKLFPFTDNQCLDWIDRSKEWYRLQKPVSKNQEPGEIKPLVLDEKAIDELYHDMLTSQSATTSKVNEGLETKVISITLGQILKTLDGKSSTFQPKVPGITKKLLQLPLYDGIAAEDELYTVDQHEYHVQLKFIPNLSSVGHIKDIPPLELWFELDDYDKAISSSVRCLLHTEDRSLLLQTPQMSHDYKVSVDRTLEATKPYEDDPQNWLKDQPGLKEFLLRARLTFQGSEKLYVPKNLEMNLRLGDSDVLQPVIFDYVNVKYRRVLRLKYMDHYLVQFSDLNGGTSGGKYTQVDFICPESNMISREAFGSFIKDVLKYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>C5DMY6</accession>
    <name>C5DMY6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KLTH0G12672p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">KLTH0G12672g</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555584.1">
        <property type="nucleotide sequence ID" value="XM_002555538.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DMY6"/>
    <dbReference type="FunCoup" id="C5DMY6">
        <property type="interactions" value="67"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DMY6"/>
    <dbReference type="GeneID" id="8293866"/>
    <dbReference type="KEGG" id="lth:KLTH0G12672g"/>
    <dbReference type="eggNOG" id="KOG4469">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030995_0_0_1"/>
    <dbReference type="InParanoid" id="C5DMY6"/>
    <dbReference type="OMA" id="QFYEDKK"/>
    <dbReference type="OrthoDB" id="1918at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014848">
        <property type="entry name" value="Rgp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12507">
        <property type="entry name" value="REDUCED GROWTH PHENOTYPE 1 RGP1, YEAST -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08737">
        <property type="entry name" value="Rgp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAR25147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="605" mass="68148" checksum="EB3653A79212B509" modified="2009-07-28" version="1">MRNYQLDTQNVAGNVKLEVIYEANPCFAGECVQAVLRLRHLGSPQIRDRLDIEMQALKQGNEESSDSAEDGGNQGLMSTFFRSFNKDQKAREVQIRQKRSRLEKDLEFHRSVSLTSCYVQIFGLFRYDPETLAPLQSDNSGSKLVGTGSLKISGRKKSNSEGLGGILFANLDDVARESMTGKKQELQKKPILLVPQTLVFSEIMLEPGEVRSFQFRSPRLPPDLPPSYKTSKNFKIQYYLNFGLTKIGSKGLTPFNTDFQIHVCPFVDAQIRQFTSKLDAGITILAPGRVKEVKDAQRSRRKSSSSILLRRRSSAQSVNFSESVSEGPKISKDLFKKLIQSRFSSDEFNDDIEGLVDKIMECQFETNNYESSEGEDTNSSQPQVRKDMGSVRENLNQLYNGTADITNTEENENEAQNGSTGPLPQIKSLQKEFIINRNGEFVGKVRLANSFYTTSDDIDLAIDLANEGRFKISAVTTSLKAFELINPKYATEALSASIKQQGQVMDENRAICFEETPTLHIKLLPHRSPTNQITSQFRTDVFQFKWMLCLKFVLLNRVENEVFVEEFYGDKNGSLSHAKQSLDGEVFSCHIPLTILPSSKNFGAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>C5DN30</accession>
    <name>C5DN30_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0G13706p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0G13706g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555628.1">
        <property type="nucleotide sequence ID" value="XM_002555582.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DN30"/>
    <dbReference type="FunCoup" id="C5DN30">
        <property type="interactions" value="621"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DN30"/>
    <dbReference type="GeneID" id="8293911"/>
    <dbReference type="KEGG" id="lth:KLTH0G13706g"/>
    <dbReference type="eggNOG" id="KOG3566">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007442_3_1_1"/>
    <dbReference type="InParanoid" id="C5DN30"/>
    <dbReference type="OMA" id="RESEWNI"/>
    <dbReference type="OrthoDB" id="445301at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042765">
        <property type="term" value="C:GPI-anchor transamidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016255">
        <property type="term" value="P:attachment of GPI anchor to protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007246">
        <property type="entry name" value="Gaa1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13304">
        <property type="entry name" value="GLYCOSYLPHOSPHATIDYLINOSITOL ANCHOR ATTACHMENT 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13304:SF0">
        <property type="entry name" value="GLYCOSYLPHOSPHATIDYLINOSITOL ANCHOR ATTACHMENT 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04114">
        <property type="entry name" value="Gaa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036762">
        <property type="entry name" value="GAA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="23"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="352"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="394"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="421"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="454"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="498"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="538"/>
            <end position="557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR25191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="584" mass="65123" checksum="E17A39FCBCF09FC1" modified="2009-07-28" version="1">MALIEKLQRRIIDLGLLPRIVRFLPKLSILCGILGICWLTLLLPLEGQYRHTYISENALMPSQAYSYFRETEWNILRGYRTQIQVLEEKSSKERNEVVASWFQEFGAKTAFYEDDLHGNTMYGVLHAPRGDGTEAMVLAAPWYNGDGEYNIGGAALATALSRFFSRWPVWSKNIIIVFSDDPEASLRSWVHAYHTSLDLTGGSIESAVVLDYPGSNDFFKYVEIYYAGLNGELPNLDLVNVAVHITEHEGMKVSLNGVSEEEMEKRNYFSKMKTMMVGVKKMALSGVQSCYGNEAFSGWRIQAIVLRARGTDGPFDITTFGRIPEATFRSVNNLLEKFHQSFFFYLLLAPRYFVSIASYLPAAVAFSVSFIVATLDCVLKSGASNTPAFSVHNLSAVIGFTVALLLSFALSILFSHWQYSSLLISCNAVIAVLPAIFGRQGIEASHSYQFKSVAFLYLSVVLTSLLVINFALAFGIGLLAFPLILVKAPMTSRQRVKNTILLILSNPFISTLLFVNTFEPQLSGLQLFHRLVQAWQKLGCWTWSVVCIGWLPSWLLVACSGIPMPYQPVGGPSQRLGSSTKKTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5DN77</accession>
    <name>C5DN77_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 9">Ribosomal RNA-processing protein 8</fullName>
            <ecNumber evidence="9">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0G14784g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the N(1) position of adenine in helix 25.1 in 25S rRNA. Required both for ribosomal 40S and 60S subunits biogenesis. Required for efficient pre-rRNA cleavage at site A2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
            <location evidence="1 9">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the methyltransferase superfamily. RRP8 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="9"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555675.1">
        <property type="nucleotide sequence ID" value="XM_002555629.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DN77"/>
    <dbReference type="FunCoup" id="C5DN77">
        <property type="interactions" value="333"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DN77"/>
    <dbReference type="GeneID" id="8293960"/>
    <dbReference type="KEGG" id="lth:KLTH0G14784g"/>
    <dbReference type="eggNOG" id="KOG3045">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027694_1_0_1"/>
    <dbReference type="InParanoid" id="C5DN77"/>
    <dbReference type="OMA" id="SCTIVVF"/>
    <dbReference type="OrthoDB" id="10258825at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016433">
        <property type="term" value="F:rRNA (adenine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2150:FF:000001">
        <property type="entry name" value="Ribosomal RNA-processing protein 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000320">
        <property type="entry name" value="Ribosomal RNA-processing protein 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2150">
        <property type="entry name" value="Ribosomal RNA-processing protein 8, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007823">
        <property type="entry name" value="RRP8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042036">
        <property type="entry name" value="RRP8_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12787">
        <property type="entry name" value="RIBOSOMAL RNA-PROCESSING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12787:SF0">
        <property type="entry name" value="RIBOSOMAL RNA-PROCESSING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05148">
        <property type="entry name" value="Methyltransf_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 9" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="9"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="52"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="71"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="95"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00076672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR25238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="417" mass="47964" checksum="17D156904AD9B74A" modified="2009-07-28" version="1">MALFEVEGWDIKTKDVSYDSANAKKENKKKERKDRAKRANERRLEAIKNAESEDEDFEENAEDDGEQVEDEERKPPTPKAEKKAKIEDSEATIQKAEKSAKKSASEPKRASRKRGAEKPEAGGDSEPAQPAKKPLTALQQKMMAKLSGSRFRWINEQLYTISSENALELIKKQPELFDEYHDGFRSQVQSWPENPVDVFVDQFRIRSKKPVNAPGGLPGLPNDKKIVVADMGCGEAQLSLDLKNFFAEANKKSKKHHKKNCVVHSFDLKKVNNRITVADIRNVPLPDGSCTVVVFCLALMGTNFLDFIKEAYRLLAPRGELWIAEIKSRFADREGEEFVNAIKLLGFFHKKTDDTNKMFTRFEFFKPPQEIIEERKAKLERKQRFVEVETEKEELESKRNKTPEGKWLLKPCIYKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNE4</accession>
    <name>C5DNE4_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">N-(5'-phosphoribosyl)anthranilate isomerase</fullName>
            <ecNumber evidence="4">5.3.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0G16280g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:21540"/>
            <dbReference type="ChEBI" id="CHEBI:18277"/>
            <dbReference type="ChEBI" id="CHEBI:58613"/>
            <dbReference type="EC" id="5.3.1.24"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TrpF family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.24" evidence="4"/>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555742.1">
        <property type="nucleotide sequence ID" value="XM_002555696.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNE4"/>
    <dbReference type="FunCoup" id="C5DNE4">
        <property type="interactions" value="176"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DNE4"/>
    <dbReference type="GeneID" id="8294028"/>
    <dbReference type="KEGG" id="lth:KLTH0G16280g"/>
    <dbReference type="eggNOG" id="KOG4202">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076364_1_0_1"/>
    <dbReference type="InParanoid" id="C5DNE4"/>
    <dbReference type="OMA" id="FHGDESP"/>
    <dbReference type="OrthoDB" id="524799at2759"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00042"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004640">
        <property type="term" value="F:phosphoribosylanthranilate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00405">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000199">
        <property type="entry name" value="Phosphoribosylanthranilate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00135">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001240">
        <property type="entry name" value="PRAI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044643">
        <property type="entry name" value="TrpF_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42894">
        <property type="entry name" value="N-(5'-PHOSPHORIBOSYL)ANTHRANILATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42894:SF1">
        <property type="entry name" value="N-(5'-PHOSPHORIBOSYL)ANTHRANILATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00697">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="N-(5'phosphoribosyl) anthranilate isomerase (PRAI)" evidence="10">
        <location>
            <begin position="30"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR25305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="239" mass="25946" checksum="F423181831FF2D7A" modified="2009-07-28" version="1">MMKLTKTKGRLASLPSKLMAFWKDADILTKICGIQSAEAAKVAIDAGASLIGIICVPNRKRTVCPVVAKEISRVVREARQGRQDQGKSGPFLVGVFRNQSISEVRSLKDDYGLDIVQLHGNENWKEYKRELEGTPLIKRFIFPNDCADVLEQSESSGMECLPLFDSEAGGTGEKLDWDAIGSWSKSSGARFILAGGLGPENVRKACELPGVMGVDVSGGVETQGNKDADKIRTFLAEAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>C5DNI4</accession>
    <name>C5DNI4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KLTH0G17314p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0G17314g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the peroxiredoxin family. Prx6 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928171">
        <property type="protein sequence ID" value="CAR25345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555782.1">
        <property type="nucleotide sequence ID" value="XM_002555736.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DNI4"/>
    <dbReference type="STRING" id="559295.C5DNI4"/>
    <dbReference type="GeneID" id="8294075"/>
    <dbReference type="KEGG" id="lth:KLTH0G17314g"/>
    <dbReference type="eggNOG" id="KOG0854">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042529_4_2_1"/>
    <dbReference type="InParanoid" id="C5DNI4"/>
    <dbReference type="OMA" id="MTPINWI"/>
    <dbReference type="OrthoDB" id="2996783at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome G"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008379">
        <property type="term" value="F:thioredoxin peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033554">
        <property type="term" value="P:cellular response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000011">
        <property type="entry name" value="Peroxiredoxin PRX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1020.10">
        <property type="entry name" value="Antioxidant, Horf6, Chain A, domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050217">
        <property type="entry name" value="Peroxiredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024706">
        <property type="entry name" value="Peroxiredoxin_AhpC-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019479">
        <property type="entry name" value="Peroxiredoxin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10681:SF121">
        <property type="entry name" value="ALKYL HYDROPEROXIDE REDUCTASE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10681">
        <property type="entry name" value="THIOREDOXIN PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10417">
        <property type="entry name" value="1-cysPrx_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000239">
        <property type="entry name" value="AHPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="1 6" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="4 6" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="8">
        <location>
            <begin position="9"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate; for peroxidase activity" evidence="7">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR000239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000239-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR25345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="222" mass="25126" checksum="464B8027CF4314FC" modified="2009-07-28" version="1">MTSHIQPQICLGATAPNFTANTSQGQLDFYSYAKGSWCLFFSHPADFTPICTTEIGAFAQLADEFEQRNCKMLCMSTNSAETHLQWILDIEDVTGAKVTFPMICDEDGRISTMFGMVDSRNFDERGLPIPIRCVLIIDPQFEVRLLHAYLLSTGRNTAEILRCLDSLQVVDQMHGKVMTPINWIPGDDVVLSPQVDQEDIERNFPEAREIRPYLRLTPLDMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>C5E246</accession>
    <name>C5E246_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="5">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">KLTH0H02068g</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cyclophilin-type PPIase family. PPIase D subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="5"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555969.1">
        <property type="nucleotide sequence ID" value="XM_002555923.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E246"/>
    <dbReference type="FunCoup" id="C5E246">
        <property type="interactions" value="1176"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E246"/>
    <dbReference type="GeneID" id="8294282"/>
    <dbReference type="KEGG" id="lth:KLTH0H02068g"/>
    <dbReference type="eggNOG" id="KOG0546">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012062_37_0_1"/>
    <dbReference type="InParanoid" id="C5E246"/>
    <dbReference type="OMA" id="EMEQNCN"/>
    <dbReference type="OrthoDB" id="193499at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016018">
        <property type="term" value="F:cyclosporin A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01926">
        <property type="entry name" value="cyclophilin_ABH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000029">
        <property type="entry name" value="peptidyl-prolyl cis-trans isomerase D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000045">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF561">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0697">Rotamase</keyword>
    <keyword evidence="8" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="11">
        <location>
            <begin position="7"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAR30107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="370" mass="40971" checksum="4637E6F29A09F2AE" modified="2009-07-28" version="1">MSRSKVFFDISIGGIPKGRMVFELYNDVVPKTAENFYKLCEGKSGNCKSKPEIPLCYKGSIFHRIIKGFMCQFGDFTNFNGTGGESIYGEKFEDENFTMKHDKPFLLSMANAGPNTNGSQAFITCVPTPHLDGKHVVFGELIQGKRIARAIESQATDSADKPLSDVKVDDCGVLPADYVVPEDAEKTPTDEFGDNYDDSLADDSKVDLENVDSVLKAVSAVKDIGTSLFKAQKFQPALTKYEKSSSMLKQYFPQDLPAEDISRVDQLRISIFLNIALCAIKTGDYQKALTSTTEVVHDAAADDKSKAKALYRRGLAYYHLKDPEMAITDLEFATTYQQNDAAILKAISDAKKLKKEQVAKQKKSLSKMFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5E252</accession>
    <name>C5E252_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ADP-ribosylation factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">KLTH0H02200g</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">GTP-binding protein involved in protein trafficking; modulates vesicle budding and uncoating within the Golgi apparatus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002555975.1">
        <property type="nucleotide sequence ID" value="XM_002555929.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E252"/>
    <dbReference type="FunCoup" id="C5E252">
        <property type="interactions" value="1259"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E252"/>
    <dbReference type="GeneID" id="8294288"/>
    <dbReference type="KEGG" id="lth:KLTH0H02200g"/>
    <dbReference type="eggNOG" id="KOG0070">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040729_9_3_1"/>
    <dbReference type="InParanoid" id="C5E252"/>
    <dbReference type="OMA" id="IRQRHWF"/>
    <dbReference type="OrthoDB" id="2011769at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04150">
        <property type="entry name" value="Arf1_5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000024">
        <property type="entry name" value="ADP-ribosylation factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045872">
        <property type="entry name" value="Arf1-5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 14" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8 14" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 11 13" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10 14" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 14" id="KW-0519">Myristate</keyword>
    <keyword evidence="5 11 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 14" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0813">Transport</keyword>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="24"/>
            <end position="31"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="126"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU369003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAR30113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="181" mass="20487" checksum="BF61F6FC15D80F03" modified="2009-07-28" version="1">MGLYISKLFNSLVGSKEMRILMVGLDGAGKTTVLYKLKLGEVVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFRNTEGIIFVVDSNDRSRISEAREVLQRMLNEDEIRNAALLVFANKQDLPEAMSAAEITEKLGLHSIRQRPWFIQATCATSGEGLYEGLEWLSNSLKNQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2E2</accession>
    <name>C5E2E2_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0H04202p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0H04202g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL12 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556065.1">
        <property type="nucleotide sequence ID" value="XM_002556019.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2E2"/>
    <dbReference type="FunCoup" id="C5E2E2">
        <property type="interactions" value="820"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2E2"/>
    <dbReference type="GeneID" id="8294378"/>
    <dbReference type="KEGG" id="lth:KLTH0H04202g"/>
    <dbReference type="eggNOG" id="KOG1715">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086499_0_1_1"/>
    <dbReference type="InParanoid" id="C5E2E2"/>
    <dbReference type="OMA" id="LEDKWGV"/>
    <dbReference type="OrthoDB" id="250175at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00387">
        <property type="entry name" value="Ribosomal_L7_L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1390.10:FF:000001">
        <property type="entry name" value="50S ribosomal protein L7/L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.710">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00368">
        <property type="entry name" value="Ribosomal_bL12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000206">
        <property type="entry name" value="Ribosomal_bL12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013823">
        <property type="entry name" value="Ribosomal_bL12_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014719">
        <property type="entry name" value="Ribosomal_bL12_C/ClpS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008932">
        <property type="entry name" value="Ribosomal_bL12_oligo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036235">
        <property type="entry name" value="Ribosomal_bL12_oligo_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45987">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45987:SF4">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL12M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00542">
        <property type="entry name" value="Ribosomal_L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16320">
        <property type="entry name" value="Ribosomal_L12_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54736">
        <property type="entry name" value="ClpS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48300">
        <property type="entry name" value="Ribosomal protein L7/12, oligomerisation (N-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein bL12 oligomerization" evidence="5">
        <location>
            <begin position="39"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein bL12 C-terminal" evidence="4">
        <location>
            <begin position="103"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF16320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR30203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="170" mass="18119" checksum="40A1B2BA55B927A2" modified="2009-07-28" version="1">MLVRGTRRSALKLARLPAVRARACVRLNSTQAAASVDPKIEKIVQDISKLTLLETSALISELKTQLNIPDIAMPVAGAAPAGAAAPAAEEAKEEEVKEEKTIFAIKLESFDAKSKPKVIKEVKNLLGLSLVEAKKFVEAAPKILKDNVAKEDADKIKASLEALGAKVALE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2G3</accession>
    <name>C5E2G3_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0H04730p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0H04730g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the membrane-bound acyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556086.1">
        <property type="nucleotide sequence ID" value="XM_002556040.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2G3"/>
    <dbReference type="FunCoup" id="C5E2G3">
        <property type="interactions" value="133"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2G3"/>
    <dbReference type="GeneID" id="8294400"/>
    <dbReference type="KEGG" id="lth:KLTH0H04730g"/>
    <dbReference type="eggNOG" id="KOG3860">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021430_1_1_1"/>
    <dbReference type="InParanoid" id="C5E2G3"/>
    <dbReference type="OMA" id="GWHRSYN"/>
    <dbReference type="OrthoDB" id="420606at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051085">
        <property type="entry name" value="MB_O-acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13285">
        <property type="entry name" value="ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13285:SF18">
        <property type="entry name" value="PROTEIN-CYSTEINE N-PALMITOYLTRANSFERASE RASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="54"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="112"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="142"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="178"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="353"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="390"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="492"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="523"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="562"/>
            <end position="582"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR30224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="597" mass="69398" checksum="B7B39A6C79326D49" modified="2009-07-28" version="1">MIVEIGNMLLQQILYFFSLKMLDSRLDPSTDAKRAQKVSMGASKSRWRTFEFKVYYVMFILVVPLMIRTAISATSERNPNYARFEPLLSKGWILGRKVDNTDSQYRFFRDNMPLLAGLIVLHTGLKKAVLRTTNVRRVRFDLVFGLLFLFAAHGVSALRVLLHLVINYAIAKTLRKNYRLATFLTWVYGISTLFFNNSYRKYPFGNVLSVLTPLDGAFKGIIERWDVFFNFTLLRMISFNMDFLEKWEQVVTGKKNNSPSPSPSHVSNLKPELKRAASSATTLETICENGTTNVLLDERARMNAPHHIQDYLFANYLAYVTYTPLFIAGPIVTFNDYLYQCQQTLPSINTKRILYYAGRFVLCVLTMEFVLHYIYAVAVSKTKAWDGDTPFQISMIGLFNLNIVWLKLLIPWRLFRLWAMIDGVDAPENMIRCVDNNFSALAFWRAWHRSFNKWVVRYVYIPLGGSKNRILTSLAVFSFVAIWHDIELKLLLWGWLIVLFLLPEIFAQRYFAPYRKRPWYRFVRAMGAILNIWMMMIANLYGFCLGYDGTVHLIKGLFFTWAGLRFLMGANVALFIAVQVMFEQREHEKRNGVDVKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2K1</accession>
    <name>C5E2K1_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase</fullName>
            <ecNumber evidence="5">2.7.8.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">GlcNAc-1-P transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">N-acetylglucosamine-1-phosphate transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ordered locus">KLTH0H05632g</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. Catalyzes the initial step of dolichol-linked oligosaccharide biosynthesis, transfering GlcNAc-1-P from cytosolic UDP-GlcNAc onto the carrier lipid dolichyl phosphate (P-dolichol), yielding GlcNAc-P-P-dolichol embedded in the cytoplasmic leaflet of the endoplasmic reticulum membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a di-trans,poly-cis-dolichyl phosphate + UDP-N-acetyl-alpha-D-glucosamine = an N-acetyl-alpha-D-glucosaminyl-diphospho-di-trans,poly-cis-dolichol + UMP</text>
            <dbReference type="Rhea" id="RHEA:13289"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19507"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:58427"/>
            <dbReference type="EC" id="2.7.8.15"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:13290"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyltransferase 4 family.</text>
    </comment>
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    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_002556078.1"/>
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    <dbReference type="KEGG" id="lth:KLTH0H05632g"/>
    <dbReference type="eggNOG" id="KOG2788">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029942_1_0_1"/>
    <dbReference type="InParanoid" id="C5E2K1"/>
    <dbReference type="OMA" id="LPHFNAR"/>
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    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
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        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003975">
        <property type="term" value="F:UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06855">
        <property type="entry name" value="GT_GPT_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000715">
        <property type="entry name" value="Glycosyl_transferase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033895">
        <property type="entry name" value="GPT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10571">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--DOLICHYL-PHOSPHATE N-ACETYLGLUCOSAMINEPHOSPHOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10571:SF0">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--DOLICHYL-PHOSPHATE N-ACETYLGLUCOSAMINEPHOSPHOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00953">
        <property type="entry name" value="Glycos_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="22"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="127"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="155"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="200"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="231"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="255"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="288"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013225"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00017659"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00029567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00033238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00044717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00045078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CAR30262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="448" mass="49764" checksum="F5E20EC06B69065B" modified="2009-07-28" version="1">MHQALLLVVAIAAIYVSRYYSPLLAALGFAIIGFLASDALIPRVSQSFIKIGCFGKDLSKPGRPVIPESIGAISATVYLFIMFFYIPFLFYKYLVVITPGGGHRDASIMQSPVSDEYRFPHGKMSEYLSAVLCLQSTVLLGIADDLFDLRWRHKFFLPAVAAIPLLVVYYVDFGVTHVLIPDVIQRWLGTSKTIFNLGALYYVYMASMAIFCPNSINILAGVNGLEVGQSIVLGVIFLINDSLYLLLGNEASKEAHLFSAILIIPFLGVAFSLWKWNKWPAKVFVGDTFCYFAGMIFSVVGILGHFSKTTLIFFIPQTFNFAYSCPQLFGLVPCPRHRMPRFNEADGLLYPSRANLVEKPPKKVFVPVLKLLSSLKLIDLEVDPQTGALKSCSNMTLINLVLVWFGPTREDKLCNRILAIQFTVGLCAIVARHSIGSIIFGHDNIQFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2M9</accession>
    <name>C5E2M9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0H06270p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0H06270g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002556106.1"/>
    </dbReference>
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    <dbReference type="FunCoup" id="C5E2M9">
        <property type="interactions" value="135"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2M9"/>
    <dbReference type="TCDB" id="9.B.235.1.2">
        <property type="family name" value="the mitochondrial outer membrane protein-14 (om14) family"/>
    </dbReference>
    <dbReference type="GeneID" id="8294467"/>
    <dbReference type="KEGG" id="lth:KLTH0H06270g"/>
    <dbReference type="eggNOG" id="ENOG502S89U">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131065_0_0_1"/>
    <dbReference type="InParanoid" id="C5E2M9"/>
    <dbReference type="OMA" id="YTERHRI"/>
    <dbReference type="OrthoDB" id="4034431at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990593">
        <property type="term" value="F:nascent polypeptide-associated complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006626">
        <property type="term" value="P:protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039454">
        <property type="entry name" value="OM14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039453">
        <property type="entry name" value="OM14_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38402">
        <property type="entry name" value="MITOCHONDRIAL OUTER MEMBRANE PROTEIN OM14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38402:SF1">
        <property type="entry name" value="MITOCHONDRIAL OUTER MEMBRANE PROTEIN OM14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17304">
        <property type="entry name" value="OM14_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="124"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="Mitochondrial outer membrane protein OM14 C-terminal" evidence="3">
        <location>
            <begin position="87"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF17304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR30290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="160" mass="17540" checksum="F84C3896EB9E01D2" modified="2009-07-28" version="1">MPNGQNEVDSSNIPDAAKRAAQELREEAQDLREEANKEFEELERNGTIDKYKDNAAGILKNVANRASAAASYVATRVKTVSQRVAYELRNPVVIVNAALGVGLVSTLLKGYTKNQRYLKGKSDSVILSTVVGATAAVALDVYLSGKYYQKFDQKSNKKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>C5E2P8</accession>
    <name>C5E2P8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein-serine/threonine kinase</fullName>
            <ecNumber evidence="7">2.7.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">KLTH0H06710g</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the PDK/BCKDK protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.-" evidence="7"/>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556171.1">
        <property type="nucleotide sequence ID" value="XM_002556125.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E2P8"/>
    <dbReference type="FunCoup" id="C5E2P8">
        <property type="interactions" value="208"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E2P8"/>
    <dbReference type="GeneID" id="8294487"/>
    <dbReference type="KEGG" id="lth:KLTH0H06710g"/>
    <dbReference type="eggNOG" id="KOG0787">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023861_0_1_1"/>
    <dbReference type="InParanoid" id="C5E2P8"/>
    <dbReference type="OMA" id="ICEAQEH"/>
    <dbReference type="OrthoDB" id="407390at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004740">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010906">
        <property type="term" value="P:regulation of glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.20">
        <property type="entry name" value="Alpha-ketoacid/pyruvate dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036784">
        <property type="entry name" value="AK/P_DHK_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018955">
        <property type="entry name" value="BCDHK/PDK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039028">
        <property type="entry name" value="BCKD/PDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947:SF25">
        <property type="entry name" value="[PYRUVATE DEHYDROGENASE (ACETYL-TRANSFERRING)] KINASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10436">
        <property type="entry name" value="BCDHK_Adom3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69012">
        <property type="entry name" value="alpha-ketoacid dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Branched-chain alpha-ketoacid dehydrogenase kinase/Pyruvate dehydrogenase kinase N-terminal" evidence="8">
        <location>
            <begin position="90"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF10436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAR30309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="494" mass="56792" checksum="F2668CC64C7923BA" modified="2009-07-28" version="1">MYKVKSKSTSGLKVKLAHRWKSQSQTQSSHISQIPEFSKYSPTKSISELAPYVSPTLCAYPLRSKYVNQQHYYQNQNILEKHYLTKKPHPVSLMQLAQYFDDSTALTKQKIVNSGNFVKQELVTRIAHKIHMLQMLPFDVVNNFHFCQVYESYYNIFERFRRYPAIKTIEDNEKFAEFLHTILSDFNSLNLPHLIMGALECRILDLYPPEKMDEVISSLLRARISRRLIVEEHLSITSNYHSGKKENTLVLGDIFQECSAKEFLLGAKSMCERFIQDMYFKGIKLPAFNIDGNTELKFLFLPLHLKYLLGEVLRNSYEATMLEYIRLGLQEPEPITVTIVSNESSFIFRISDRAGGIPHDEKTIWSFGKSKELARQSLSNFHKLPGLQTISLYDSVNGKGLTRSVDHPYKYTSLEPINASNLPSGLHKFEKPLLGLLERSSRYKLGIGLAMCKVYAEYWNGTLTVHSIPGYGTDTILKLGNLMYHTGKLQLDKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>C5E352</accession>
    <name>C5E352_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0H10450p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0H10450g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the VPS26 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556325.1">
        <property type="nucleotide sequence ID" value="XM_002556279.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E352"/>
    <dbReference type="FunCoup" id="C5E352">
        <property type="interactions" value="887"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E352"/>
    <dbReference type="GeneID" id="8294656"/>
    <dbReference type="KEGG" id="lth:KLTH0H10450g"/>
    <dbReference type="eggNOG" id="KOG3063">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031077_2_0_1"/>
    <dbReference type="InParanoid" id="C5E352"/>
    <dbReference type="OMA" id="FKWKFSS"/>
    <dbReference type="OrthoDB" id="3821113at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030904">
        <property type="term" value="C:retromer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.640:FF:000015">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028934">
        <property type="entry name" value="Vps26-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12233">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING 26 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03643">
        <property type="entry name" value="Vps26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR30463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="312" mass="35643" checksum="C55B54F5BB5049DB" modified="2009-07-28" version="1">MSLFFKSPIDIEVLLDGEESRKHVEVANASNNSTAKTLKDRFPVYEDGESVSGIVTLRVKEGRKLEHLGVKVSLIGSVDISKSSSDYKKRPVDTFLSLSADLCPQGELMHSQSFPFSFKDVEKRYDSYRGKNVDVSYYVKVTVLRKSTDVVKFKRFWCLLYNSEPPAPALSKSSDDAKPVKLDIGIENCLHIEFEYSKSQYSLKDVIVGRIYFLLTRLKVKHMELSLVTRETCGLEPNQLSDTTSIRYEIMDGSPVKGETIPIRLFLGGYDLTPNSNFNHFSIRNYLSLVIIDEDGRRYFKQSEIVLYRKRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>C5E391</accession>
    <name>C5E391_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0H11330p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0H11330g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556364.1">
        <property type="nucleotide sequence ID" value="XM_002556318.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E391"/>
    <dbReference type="FunCoup" id="C5E391">
        <property type="interactions" value="282"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E391"/>
    <dbReference type="GeneID" id="8294695"/>
    <dbReference type="KEGG" id="lth:KLTH0H11330g"/>
    <dbReference type="eggNOG" id="ENOG502QVSC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014696_0_0_1"/>
    <dbReference type="InParanoid" id="C5E391"/>
    <dbReference type="OMA" id="YWYEMCH"/>
    <dbReference type="OrthoDB" id="349045at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028942">
        <property type="entry name" value="WHIM1_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15612">
        <property type="entry name" value="WHIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WHIM1" evidence="4">
        <location>
            <begin position="322"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="676"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="36"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="54"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="89"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="99"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="694"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="717"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="766"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="805"/>
            <end position="831"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF15612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR30502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="831" mass="94424" checksum="B6FA70B9B9FE43DA" modified="2009-07-28" version="1">MSDPLESDSKSSSEPSLEATVSEGTNSKSKPKKIQLTFQDFKNIKSSTQSPEGLLKHATDMLESNRRRSRRVVPKRPVDEDDEETSRAAPKKKKKPAVKSKEPNINREAVRERVKKQQQAIENPETAILEENWSPNVPLPSSDFKNHYSIVSRFRSPNMKSVPYAKDVIQIMTFINKFSGFLPRDLWSLSFQDFELGLDLYPESDKETPLKLYQDFMPIKDLVKCQDAMNLLFLSLMKLALNNNTPTSSKSLSEGPKPYSKFLGQLRTNAWAWGYPKEWKFQVGDAHSKVFTDDDTEPVDPNNHEILTPNLPTWPQQYAIDSDPLLTPELEKLGILALSPSDRIVLLRILVQWCLSQSDKIHTEVFRLSHLKRDAPYGVPTYHVPRIFLEGEIPTKAKFKRLCELVQARLEIKSTKKHVKKQLEHGKRADLSQKLALIKSIEQSREEVHSNGEGEIAPPVDIFDREFDSWNEIIDGELPDHPMSSPYEDEIFKLRDLEFFIGRVPHVGDFYIPRLHTYQEAKKRLIPTTYTDPRSLARTLDSFANNEVDTLTLFTQNGKLMSTHFKVFYHDSPAMIRDVASQSTTRPKTYWYEMCHDCNTLREFITLLDYKLNLLESPGSEPANKGLINKNPLPKESKFNASREKLRSVRDYLTKLLPFLAAFEELDAKFDEIESSKRTLRRSQRNRATYSKDDDLDDAYSEDEAYVEKEGGSGDELGQESESDAYADEDFPEEDDGNDDFTDDENDDESYELRGAKPKIATTSRGRGRGRGRGRGRGRGRGRGKGGSTRGGTTTEGRGGARGSGRGRGRGRGRGRGRGRGRGRGRGRGGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3C7</accession>
    <name>C5E3C7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0H12254p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0H12254g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DCC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556400.1">
        <property type="nucleotide sequence ID" value="XM_002556354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3C7"/>
    <dbReference type="FunCoup" id="C5E3C7">
        <property type="interactions" value="464"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3C7"/>
    <dbReference type="GeneID" id="8294737"/>
    <dbReference type="KEGG" id="lth:KLTH0H12254g"/>
    <dbReference type="eggNOG" id="KOG0798">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034504_0_0_1"/>
    <dbReference type="InParanoid" id="C5E3C7"/>
    <dbReference type="OMA" id="DSESWPF"/>
    <dbReference type="OrthoDB" id="276989at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031390">
        <property type="term" value="C:Ctf18 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034088">
        <property type="term" value="P:maintenance of mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019128">
        <property type="entry name" value="Dcc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13395:SF6">
        <property type="entry name" value="SISTER CHROMATID COHESION PROTEIN DCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13395">
        <property type="entry name" value="SISTER CHROMATID COHESION PROTEIN DCC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09724">
        <property type="entry name" value="Dcc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR30538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="375" mass="42236" checksum="36221C49BD6E7CD2" modified="2009-07-28" version="1">MPASALYSKLHTDADYKLLQLDSSLLKALENEGKLAFKATKESDSVVLCSKSNTWTLKQKNHSNTVLIMREFVPEEVPQTENWSTLGVNEPSTFSLGFTKQSYELEPRPTAGEISYSTLPIYSGEAHKAPNASGLSNAACLSLEDLRNCSPCSDEEFAEKWPALGGCVVNGAACILSREFISRALHITLMSCMAESLDFDDLDEPQAFAAVNKDMGEAGEFNPFSKEVISTVLGKFCSWSPGGRFKLQKETISQWYGIEALKKFASRSSIPQEEFMIKWKSLFPPYFSCDLDFKALRGYYYRPLGSCIQYFSKETLPDDPKERFSYLFKLQSTWDLDEIAPFIDQLNTKGVKIESFVMKFARRRRQGKRVTVSAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3G9</accession>
    <name>C5E3G9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">KLTH0H13442p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">KLTH0H13442g</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556442.1">
        <property type="nucleotide sequence ID" value="XM_002556396.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3G9"/>
    <dbReference type="FunCoup" id="C5E3G9">
        <property type="interactions" value="1028"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3G9"/>
    <dbReference type="GeneID" id="8294801"/>
    <dbReference type="KEGG" id="lth:KLTH0H13442g"/>
    <dbReference type="eggNOG" id="KOG0062">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_36_6_1"/>
    <dbReference type="InParanoid" id="C5E3G9"/>
    <dbReference type="OMA" id="CTHIADI"/>
    <dbReference type="OrthoDB" id="2110130at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03221">
        <property type="entry name" value="ABCF_EF-3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000104">
        <property type="entry name" value="ATP-binding cassette sub-family F member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000882">
        <property type="entry name" value="Translation initiation regulator (Gcn20)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032781">
        <property type="entry name" value="ABC_tran_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050611">
        <property type="entry name" value="ABCF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211:SF117">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY F MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211">
        <property type="entry name" value="ATP-BINDING TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12848">
        <property type="entry name" value="ABC_tran_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="199"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="532"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="283"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAR30580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="752" mass="85047" checksum="5A386ED14C4E0C94" modified="2009-07-28" version="1">MSNIGLKIRQAAPAVDQVVADYAKGYYNHLLNATYDAVQSKQLALSKEISFLQELLVNAGGNEEKVEKVCHEMLDSLSHQLQENQSKLEVTGDTSKRLLDVNVLQHHNSKANINTSLTNLGVSGDIEHTGRNMETRVDLKKLEKAEKKIAKKVAKRNNKFVKYEASRLINQSKEDDYDSFFLQINPLEFGSAAGKSKDIKVDTFDLYVGDGQRILSDAQLTLSFGHRYGVVGQNGIGKSTLLRALSRRELNVPKHISILHVEQELRGDETKALQSVLDADVWRKQLLSEEAKINERLTEIEKLRSEFEEESLEARKLENERSDLENHLEQISEKLIDMESDKAEARAASILYGLGFSTESQQQPTNSFSGGWRMRLSLARALFCQPDLLLLDEPSNMLDVPSIAYLSEYLKTYPATVVVVSHDRAFLNEVATDIIYQHNERLDYYRGQNFDSFYATKEERRKNAQREYENQLAYRKHLQEFIDKYRYNAAKSQEAQSRIKKLEKLPVLEPPEEEKNIDFHFPDCEKLSPPIIQLQDVSFGFDPSQLLLKDVNLDVQMDSRIAIVGANGCGKTTLLKILMEQLRPLQGYVSRNPRLRVAYFTQHHVDSMDLNASAVDWMSNTFPGRTDEEYRRHLGAFGITGSLGLQRMQLLSGGQKSRVAFAALCLNNPHILVLDEPSNHLDTAGLDALMDALKKFTGGVLMVSHDISVIDGVCNEIWVSENGTVRRFEGTIFDYKNYIIASAGKAGVVKKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3I4</accession>
    <name>C5E3I4_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KLTH0H13816p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">KLTH0H13816g</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556457.1">
        <property type="nucleotide sequence ID" value="XM_002556411.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3I4"/>
    <dbReference type="FunCoup" id="C5E3I4">
        <property type="interactions" value="901"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3I4"/>
    <dbReference type="GeneID" id="8294818"/>
    <dbReference type="KEGG" id="lth:KLTH0H13816g"/>
    <dbReference type="eggNOG" id="KOG0416">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030988_7_1_1"/>
    <dbReference type="InParanoid" id="C5E3I4"/>
    <dbReference type="OMA" id="VTQFFVE"/>
    <dbReference type="OrthoDB" id="269518at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23797">
        <property type="entry name" value="UBCc_UBE2H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000016">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="6">
        <location>
            <begin position="3"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="157"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="162"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="207"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="3">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAR30595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="233" mass="26173" checksum="478E0A13B9693DCD" modified="2009-07-28" version="1">MSNSKRRIETDVMKLLMSDHDVELVDDNMQEFHIKFHGPQGTPYEAGVWRLHVELPDNYPYKSPSIGFVNKIFHPNIDAASGSICLDVINSTWSPLYDLINIVEWMIPGLLKEPNGSDPLNNEAATLQLKNKELYEQKIREFIDKYATEERYRSMFGVGKSDDEDEDGGDDDDAEDDADVGNDTVDDATLDNLSDADFSDELSNIEVSDDDDGVDDEDEDVDDPGLSDADLDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3I8</accession>
    <name>C5E3I8_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 10">Vacuolar protein 8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0H13926g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 10">Functions in both vacuole inheritance and protein targeting from the cytoplasm to vacuole.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Vacuole membrane</location>
            <topology evidence="1 10">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the beta-catenin family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556461.1">
        <property type="nucleotide sequence ID" value="XM_002556415.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3I8"/>
    <dbReference type="FunCoup" id="C5E3I8">
        <property type="interactions" value="146"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5E3I8"/>
    <dbReference type="GeneID" id="8294823"/>
    <dbReference type="KEGG" id="lth:KLTH0H13926g"/>
    <dbReference type="eggNOG" id="KOG4224">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021483_0_0_1"/>
    <dbReference type="InParanoid" id="C5E3I8"/>
    <dbReference type="OMA" id="VWDKPDG"/>
    <dbReference type="OrthoDB" id="7537227at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043495">
        <property type="term" value="F:protein-membrane adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071562">
        <property type="term" value="P:nucleus-vacuole junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000011">
        <property type="term" value="P:vacuole inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000131">
        <property type="entry name" value="Vacuolar protein 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045156">
        <property type="entry name" value="Vac8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47249">
        <property type="entry name" value="VACUOLAR PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47249:SF1">
        <property type="entry name" value="VACUOLAR PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 10" id="KW-0926">Vacuole</keyword>
    <feature type="repeat" description="ARM" evidence="9">
        <location>
            <begin position="85"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="9">
        <location>
            <begin position="126"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="9">
        <location>
            <begin position="167"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="9">
        <location>
            <begin position="208"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="9">
        <location>
            <begin position="251"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="9">
        <location>
            <begin position="292"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="9">
        <location>
            <begin position="333"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00026209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU369097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR30599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="563" mass="61002" checksum="B53A81A37865EC69" modified="2009-07-28" version="1">MGACCSCLNESRDDASVLPIADNEREAVTSLLGYLEDKDRFDFYSGGPLKALTTLVYSDNLNLQRSAALAFAEITEKYVRPVNREVLEPILILLQSSDSQIQVAACAALGNLAVNNENKILIVDMGGLEPLINQMMSSNVEVQCNAVGCITNLATQDGNKAKIATSGALVPLTKLAKSKNIRVQRNATGALLNMTHSGENRRELVNAGAVPVLVALLSSVDADVQYYCTTALSNIAVDESNRKKLSQTEPRLVSKLVALMDSPSARVKCQATLALRNLASDTGYQLEIVRAGGLPHLAKLIQSDSMPLVLASVACIRNISIHPLNEGLIVDAGFLKPLVKLLDFKASEEIQCHAVSTLRNLAASSEKNRQEFFESGAVEKCKELALDSPMSVQSEISACFAILALADNSKVDLLDSNILEALIPMTFSKNQEVSGNAAAALANLCSRINNYARIIESWETPGDGIRGFLIRFLRSEYPTFEHIALWTILQLLESHDETVLKLIKGNKEIIKNIKHLSEVNFESAQKASAAMNSQAGSGSGSDQFDDASLELYNITQQIIQFID</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3R7</accession>
    <name>C5E3R7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KLTH0H15774p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">KLTH0H15774g</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556540.1">
        <property type="nucleotide sequence ID" value="XM_002556494.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3R7"/>
    <dbReference type="FunCoup" id="C5E3R7">
        <property type="interactions" value="58"/>
    </dbReference>
    <dbReference type="GeneID" id="8294906"/>
    <dbReference type="KEGG" id="lth:KLTH0H15774g"/>
    <dbReference type="eggNOG" id="ENOG502QQFN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054590_1_0_1"/>
    <dbReference type="InParanoid" id="C5E3R7"/>
    <dbReference type="OMA" id="CATCFFP"/>
    <dbReference type="OrthoDB" id="58297at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002925">
        <property type="entry name" value="Dienelactn_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47562">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47562:SF2">
        <property type="entry name" value="CARBOXYMETHYLENEBUTENOLIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01738">
        <property type="entry name" value="DLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dienelactone hydrolase" evidence="1">
        <location>
            <begin position="28"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAR30678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="273" mass="30745" checksum="5BE5ED94F2906877" modified="2009-07-28" version="1">MLIEESYRDVKTSYNTSLRLYTFTPKIAGHPHAKFPGVLVFSEIYQVTGPVRRFAQRIAAQGYVVVAPAIYHNFVGPEALAYDAEGTDMGNEFKIKKPLESYDEDSKLCCDYLYELPQFNGKRIGATGMCLGGHLAFRSLLDKRVTCATCFFPTDIHSKTLGLGQNDDSLERVANEATKDQELVLIFGTLDTHVSPEGRDLIRKQLRDHGVNVTFMEIHAAQHAFIRDESSKGRYDAAITESCLGFMFEQFNRRLALDLGDFVEDNKKVEHVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-02-05" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>C5E3T6</accession>
    <name>C5E3T6_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0H16192p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0H16192g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928180">
        <property type="protein sequence ID" value="CAR30697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002556559.1">
        <property type="nucleotide sequence ID" value="XM_002556513.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5E3T6"/>
    <dbReference type="GeneID" id="8294925"/>
    <dbReference type="KEGG" id="lth:KLTH0H16192g"/>
    <dbReference type="eggNOG" id="KOG2504">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_1_2_1"/>
    <dbReference type="InParanoid" id="C5E3T6"/>
    <dbReference type="OMA" id="AWTFRIM"/>
    <dbReference type="OrthoDB" id="2213137at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome H"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF315">
        <property type="entry name" value="TRANSPORTER MCH2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="145"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="176"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="200"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="232"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="264"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="314"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="379"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="406"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="437"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="470"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="51"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="66"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR30697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="539" mass="59361" checksum="2C3AA499C4C1CDEA" modified="2009-07-28" version="1">MPENYSSRQHASAKNAGDRDEASSIQTPVFTRGYSLELTSSPAAHVVSNNIEKLKDIEDQTTSSPKSDKENADFNENTRDMEDDNGLYAKDEYLDPPPDGGYGWVSCACVSLLNFATWGPNTSWGVFLSYFLSSNYFPGATPTDFAIIGGLVIGLTLFLLPVVAAAMSLFGYKPTLAFAIALEAVAFVASSFVKSVGMLYFTYGVLLGISFGVIFGSNTIVIPGWFLKKRALANGFGHMGVGFGGLVYSFAVRAMIDRTGDHKWAMRMLAASTFFLNIISLYFIRVRKPRIPQPKKSVKEIMQQTFDKSVLKIVPLHLCMLWASFASIGYVILLFSMSNFATSMGMTSQQATIALAVFNGSQAIGRPFMGWISEIVGRCNATILIMVYDLILILPFWLNITKFSELVPFCFLLGFGVGVGSVNIVPLVSDVVGIQKFAAGIGYGLFGNGVVSIFAEVIGLHLRTPSSSRPYLHCQIFVACMFFGGLLCLMPYRELKLRRVLAAKVRNSSSTEQQKAYYQELQQKTPYNYIKRMFFIVKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBN5</accession>
    <name>C5DBN5_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0A04092p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0A04092g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551634.1">
        <property type="nucleotide sequence ID" value="XM_002551588.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBN5"/>
    <dbReference type="FunCoup" id="C5DBN5">
        <property type="interactions" value="175"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBN5"/>
    <dbReference type="GeneID" id="8290437"/>
    <dbReference type="KEGG" id="lth:KLTH0A04092g"/>
    <dbReference type="eggNOG" id="KOG3986">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017894_0_0_1"/>
    <dbReference type="InParanoid" id="C5DBN5"/>
    <dbReference type="OMA" id="KSKRFQN"/>
    <dbReference type="OrthoDB" id="1881at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000164">
        <property type="term" value="C:protein phosphatase type 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001069">
        <property type="term" value="F:glycogen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008157">
        <property type="term" value="F:protein phosphatase 1 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005979">
        <property type="term" value="P:regulation of glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2440">
        <property type="entry name" value="Carbohydrate binding type-21 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005036">
        <property type="entry name" value="CBM21_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038175">
        <property type="entry name" value="CBM21_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050782">
        <property type="entry name" value="PP1_regulatory_subunit_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12307:SF36">
        <property type="entry name" value="GLYCOGEN-BINDING SUBUNIT 76A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12307">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03370">
        <property type="entry name" value="CBM_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51159">
        <property type="entry name" value="CBM21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBM21" evidence="2">
        <location>
            <begin position="272"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR21192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="432" mass="48431" checksum="7EBA0D244433A330" modified="2009-07-28" version="1">MYIRRPPSDTNGDVMNRSLAFLHKPQRVSQPKPFAEESLRRNTDLNRGKVQVNEMHDDYFSSQTPASADGSVAGDVSSQLRVNPYFPANENYYYNSSGDGDDADNEPPPLVRKKSGEVVRSNLKLAQRSRSLPATPSGREPDKRIGAPPPGPQRSKSVHFDQRDVVKFKYFWQEDSPLDVASQDVLEGPLDEDPHTLTLQADAAVRSDGSDAEPRDLTMTMSNLQIEKRMSSGLRRSKRYQRLRSKGAEKDGATKPGLYRENFATLSDDDQSSLKSNIFLNIAHNSYCFLQDLSLIADQHYLVGHVLVKNIFYDKEVVVRYTRDHWRTVHEVECVWESSGDDVLPGMGMDRFKLVIDLATLGDDGGAAPRAPRAGPAAAFALVKLEFCIRYTTRSSHERVEHWDNNGGRNYVVDVVCAAQRMGFTDPFGPPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBS1</accession>
    <name>C5DBS1_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KLTH0A04906p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">KLTH0A04906g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551670.1">
        <property type="nucleotide sequence ID" value="XM_002551624.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBS1"/>
    <dbReference type="FunCoup" id="C5DBS1">
        <property type="interactions" value="137"/>
    </dbReference>
    <dbReference type="GeneID" id="8290474"/>
    <dbReference type="KEGG" id="lth:KLTH0A04906g"/>
    <dbReference type="eggNOG" id="ENOG502QSY4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029536_0_0_1"/>
    <dbReference type="InParanoid" id="C5DBS1"/>
    <dbReference type="OMA" id="NWRKKWG"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47932">
        <property type="entry name" value="ATPASE EXPRESSION PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47932:SF63">
        <property type="entry name" value="OS08G0290000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAR21228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="637" mass="73993" checksum="D7C47B6BE687BB03" modified="2009-07-28" version="1">MVRKATIVNLTTFLRGLHVQVPNCASQLRDRITHFERNHEVLRKSRSQLKRIEERRRLDLKNLNKTPYSQQHARHVLKRKYEVPSEDLDSTKLGPTSESDLRFLKLTHDKRLLYTILGVTGEQLRDSKLIAADVSKFLSRNQLEKAVFLARLAKSRGVVGMNKVIEHFCQVKQDAGSAIDMYNWRKKWDIPPNEFTHTILFDGLANVKQPLSEKQSQRVLKIAKLLLEREEMNIAGFNASLSALANSVNVEYAFELFDARKKQISPDAITYTILLRALSLTQDDKLCMARLDAVMASVPRKFLDGRVVYEYCHVLHNRKDSMLSKMAIKALANFFDINIPLEKISFPAGVNLPSLQFWDVKQRFDSNKFVADLFFQNCERNKLYELAWETYKAWNSENPRLLELPVHERMIRIVTAAFPTKCAEMATEVFKNVNLRHRSGKNGMLLVYRAFERQAGKKFINDSESNVDKLLTQLFDFSIELEGKHWKELGTRVLNWKAWMFCWNVVNKANERRKISNERAKWILDQYLRTILTGQTNRAGIQHEDHAGLRHVGLESVRFLSSFADHFKIKGESELNDFVSEHDRQFFLYRRLLLRFKDRILEQVKILEGRPGSAESVEESIKQTAEKLLATKLPDQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBT9</accession>
    <name>C5DBT9_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KLTH0A05302p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">KLTH0A05302g</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551688.1">
        <property type="nucleotide sequence ID" value="XM_002551642.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBT9"/>
    <dbReference type="FunCoup" id="C5DBT9">
        <property type="interactions" value="642"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBT9"/>
    <dbReference type="GeneID" id="8290492"/>
    <dbReference type="KEGG" id="lth:KLTH0A05302g"/>
    <dbReference type="eggNOG" id="KOG3275">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056776_3_0_1"/>
    <dbReference type="InParanoid" id="C5DBT9"/>
    <dbReference type="OMA" id="YRVVMNC"/>
    <dbReference type="OrthoDB" id="672793at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01277">
        <property type="entry name" value="HINT_subgroup"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039384">
        <property type="entry name" value="HINT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019808">
        <property type="entry name" value="Histidine_triad_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001310">
        <property type="entry name" value="Histidine_triad_HIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011146">
        <property type="entry name" value="HIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46648:SF1">
        <property type="entry name" value="ADENOSINE 5'-MONOPHOSPHORAMIDASE HNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46648">
        <property type="entry name" value="HIT FAMILY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01230">
        <property type="entry name" value="HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00332">
        <property type="entry name" value="HISTRIAD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00892">
        <property type="entry name" value="HIT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51084">
        <property type="entry name" value="HIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HIT" evidence="4">
        <location>
            <begin position="15"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Histidine triad motif" evidence="2 3">
        <location>
            <begin position="110"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-AMP-histidine intermediate" evidence="1">
        <location>
            <position position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR601310-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR601310-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAR21246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="154" mass="16978" checksum="04BB735B7A004D57" modified="2009-07-28" version="1">MSAPVAHDAACIFCKIIKATTGEIPSFKLIETKYSFSFLDIQPTAEAHALVIPKFHGAKLHNVPDEYLADILPVTKKLAQATGLAIDSPEGVGYNVLQNNGRIAHQIVDHVHFHLIPKRDEKTGLVVGWPSLETDFEKLGQLHKELLAKIEAHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-06-18" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>C5DBX7</accession>
    <name>C5DBX7_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KLTH0A06160p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">KLTH0A06160g</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL19 family.</text>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551726.1">
        <property type="nucleotide sequence ID" value="XM_002551680.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DBX7"/>
    <dbReference type="FunCoup" id="C5DBX7">
        <property type="interactions" value="311"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DBX7"/>
    <dbReference type="GeneID" id="8290531"/>
    <dbReference type="KEGG" id="lth:KLTH0A06160g"/>
    <dbReference type="eggNOG" id="KOG1698">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076387_2_0_1"/>
    <dbReference type="InParanoid" id="C5DBX7"/>
    <dbReference type="OMA" id="TYVELRV"/>
    <dbReference type="OrthoDB" id="432645at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001857">
        <property type="entry name" value="Ribosomal_bL19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038657">
        <property type="entry name" value="Ribosomal_bL19_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15680:SF9">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL19M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15680">
        <property type="entry name" value="RIBOSOMAL PROTEIN L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01245">
        <property type="entry name" value="Ribosomal_L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAR21284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="169" mass="19639" checksum="237BB65093B5F4C6" modified="2009-07-28" version="1">MLGFRGFSKTFSRGYQVKSTTFKTIPVYPAVQSPSNHNLLSSLSKQELDKKLDPEGWRRSLISSHNKERLRAGDVVRVAYNQQKCKYDNLMGYVLSVDRKALEQDASILLRNQYSKTFVEVRVPIFSPLVERIDLIRRADGRRQRSKHYYIRGTKLDVGDLEAGMRKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>C5DC30</accession>
    <name>C5DC30_LACTC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">KLTH0A07370p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">KLTH0A07370g</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551779.1">
        <property type="nucleotide sequence ID" value="XM_002551733.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DC30"/>
    <dbReference type="FunCoup" id="C5DC30">
        <property type="interactions" value="75"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DC30"/>
    <dbReference type="GeneID" id="8290586"/>
    <dbReference type="KEGG" id="lth:KLTH0A07370g"/>
    <dbReference type="eggNOG" id="ENOG502RS1K">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_263670_0_0_1"/>
    <dbReference type="InParanoid" id="C5DC30"/>
    <dbReference type="OMA" id="RNCKREC"/>
    <dbReference type="OrthoDB" id="4036684at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025928">
        <property type="entry name" value="Flocculin_t3_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13928">
        <property type="entry name" value="Flocculin_t3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012429320" evidence="7">
        <location>
            <begin position="16"/>
            <end position="1274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="181"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="292"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="418"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="605"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="800"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="910"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1059"/>
            <end position="1078"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1086"/>
            <end position="1149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="181"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="196"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="213"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="228"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="299"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="811"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="849"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="914"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="932"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1060"/>
            <end position="1076"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1086"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1103"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1127"/>
            <end position="1142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAR21337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="1274" mass="132123" checksum="C21879C3691914B3" modified="2009-07-28" version="1">MKLAFVLTSCSTALASTYPRVYYANTSTVKQSATSYRELTSLVLSSTHSDGLVSTFVSEAFVSLTTLTTNDVVTVVTTWCPLGSEVVNSQLSSYSEESRASSSNLNAPTTTSPTSVFIDMSGTTETIQKSQIHTKEITTCPTCSVRPTSASTVPESTDIASSSVASSISHSKDLVSQAGSSLSSSAKSSSARMPSITTSGEIGTSAVDTGNLSPSSSSNSYQSGSSSTETHATTSQNTLSSVSGLSKSAYSPGLSSFDVRPVLTTETVDGKTTVYTTWCPLTSHSISSLYSVVTSDNPSSPSSVRIASSESQVSDENEHESDFSTQASSLQEHGYSSYSMVTITSTSLRTVVYSTELCSSRRCSPSLTSVLESIVFTTSVHPTGLGSSSERSRNTYSNSKTDSILKGQLCSLTSCQETSTSDTSGTGSSSKPTSVSQSATSEAAESSKSLSEGYQSSVSSKSFATSQSCVSCASQRNYYTTESETYLSEKASLTSAESSQKLSLSSFSSVPDSSVSSSLLSTYSIVTTTSNGVVTVYTTWCPLSNLKSSSNNGLVSSSLALKIVSTEQSGTKKLSSTQQYRSLSKTASSFMTLGSGALTFSTPSVTNSKPISGSTLASSTQRPSVETHNFIQRSSFSASHFSSLVATSHGISGSIDTVQSGSSTSLSTSSAISTEHLSNTRYSSISEGSNSQLQKTSTGSLISVLSSSSRSVPVVSTANSKNEETSCTQCQTSSHTSFSTEISSIVSLSVPSQSQDLKTSARSSTSLKSSVTYTSKVITTTSSGIRTVYTTYCPWTSSASQESQNSEKQKSSAASSSASSPASSPASSSASSQQELSSSVASTSESQKHQTSSSLDSEPQRSSKATIQSVGSQASSSKTWTSKVVTTTEGGVTTIYTTWCPQEDLQSSGTVAAVSPSTSQQKSSSLVTTTSESQTHETPSSLDSESQRSSKATIQSVGSQASSSKTWTSKVVTTTEGGVTTIYTTWCPLEDLHSGTVAAASLPSKFTDSTYISSTSSPSGLSSVPSINEGKTASSALKSSEIAKTLSDSSAKELTLYGTSSSMKASEAGSSDSMSSTIVPRMSTAESSAIIQSTQDASKKSSKGTVTTGRPSTSNVERSPSTTLGTTESLVSSSHTFSSTKTPAVSPHSSAIISSKATTYSSSFTISVTTVLPFTKTSTGDNGQLFTVVSSQPSTIPTVTTVLVKTRVPVTTYSQVSITESASQQATRKSSTEDTTSSWAIASTVSTYEGSAASTRRFDRILKVATALLLTFIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2025-10-08" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5DC44</accession>
    <name>C5DC44_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Nucleolar GTP-binding protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">KLTH0A07678g</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 8">Nucleus</location>
            <location evidence="2 8">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the TRAFAC class YlqF/YawG GTPase family. NOG2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CU928165">
        <property type="protein sequence ID" value="CAR21351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551793.1">
        <property type="nucleotide sequence ID" value="XM_002551747.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DC44"/>
    <dbReference type="FunCoup" id="C5DC44">
        <property type="interactions" value="971"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DC44"/>
    <dbReference type="GeneID" id="8290600"/>
    <dbReference type="KEGG" id="lth:KLTH0A07678g"/>
    <dbReference type="eggNOG" id="KOG2423">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011106_4_0_1"/>
    <dbReference type="InParanoid" id="C5DC44"/>
    <dbReference type="OMA" id="RTQGFNH"/>
    <dbReference type="OrthoDB" id="444945at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome A"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01858">
        <property type="entry name" value="NGP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000559">
        <property type="entry name" value="Nuclear/nucleolar GTPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1580.10:FF:000005">
        <property type="entry name" value="Nucleolar GTP-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030378">
        <property type="entry name" value="G_CP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024929">
        <property type="entry name" value="GNL2_CP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023179">
        <property type="entry name" value="GTP-bd_ortho_bundle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012971">
        <property type="entry name" value="NOG2_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050755">
        <property type="entry name" value="TRAFAC_YlqF/YawG_RiboMat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11089">
        <property type="entry name" value="GTP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11089:SF9">
        <property type="entry name" value="NUCLEOLAR GTP-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08153">
        <property type="entry name" value="NGP1NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51721">
        <property type="entry name" value="G_CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="CP-type G" evidence="10">
        <location>
            <begin position="212"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="459"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="470"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAR21351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="502" mass="57440" checksum="6A66F0664AA6CC81" modified="2009-07-28" version="1">MGTGKKEKQRRVRQNDTRDGNLRVKGENFYRDAKKVQFLNMYTDGKSIRNKKGDLIRAAPLQGVEVPTARVQPDRRWFGNTRVISQDALTHFREALGDTEKNNYQVLLRRNKLPLSLLDEKDVQESPTAKILETESFEQTFGPKSQRKKPRVAASSLEELAENAVKHNEQFEEKRELDSTLGLMAQNEQNEDGWTQTAKEAIFSKGQSKRIWNELYKVIDSSDVVIHVLDARDPLGTRCKSVEEYMRKETPHKHLIFVLNKCDLVPTWVAAAWVKHLSSERPTLAFHASITNSFGKGSLIQLLRQFSLLHKDRKQISVGFIGYPNTGKSSIINTLRKKKVCQVAPIPGETKVWQYITLMKKIFLIDCPGIVPPSAKDTEEDVLFRGVVRVEHVSHPEQYIPGVLRRCQRKHLERTYEISGWKDSVDFIEMLARKQGRLLKGGEPDESGVSKQVLNDFNRGKIPWFMPPPEKVERSAEQDSSQKKRSAQDQSSSDQHAKKIKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCD2</accession>
    <name>C5DCD2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Crh-like protein</fullName>
            <ecNumber evidence="18">3.2.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ordered locus">KLTH0B02090g</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1-&gt;4)-beta-linkages in chitin and chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.14"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
            <topology evidence="3">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the glycosyl hydrolase 16 family. CRH1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.2.-.-" evidence="18"/>
    <dbReference type="EMBL" id="CU928166">
        <property type="protein sequence ID" value="CAR21443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002551881.1">
        <property type="nucleotide sequence ID" value="XM_002551835.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C5DCD2"/>
    <dbReference type="FunCoup" id="C5DCD2">
        <property type="interactions" value="46"/>
    </dbReference>
    <dbReference type="STRING" id="559295.C5DCD2"/>
    <dbReference type="GeneID" id="8290710"/>
    <dbReference type="KEGG" id="lth:KLTH0B02090g"/>
    <dbReference type="eggNOG" id="ENOG502QQ71">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027506_2_2_1"/>
    <dbReference type="InParanoid" id="C5DCD2"/>
    <dbReference type="OMA" id="DDDNWEG"/>
    <dbReference type="OrthoDB" id="4781at2759"/>
    <dbReference type="Proteomes" id="UP000002036">
        <property type="component" value="Chromosome B"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008843">
        <property type="term" value="F:endochitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02183">
        <property type="entry name" value="GH16_fungal_CRH1_transglycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000162">
        <property type="entry name" value="Glycosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017168">
        <property type="entry name" value="CHR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050546">
        <property type="entry name" value="Glycosyl_Hydrlase_16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963:SF68">
        <property type="entry name" value="GLYCOSIDASE CRH1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963">
        <property type="entry name" value="GLYCOSYL HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00722">
        <property type="entry name" value="Glyco_hydro_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037299">
        <property type="entry name" value="Glycosidase_CRH1_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0134">Cell wall</keyword>
    <keyword evidence="16" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="12 20" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="10 18" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="11 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GH16" evidence="22">
        <location>
            <begin position="18"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="290"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="19">
        <location>
            <position position="119"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="19">
        <location>
            <position position="123"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="24"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00038074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRNR" id="PIRNR037299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR037299-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR037299-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="CAR21443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000002036"/>
        </source>
    </evidence>
    <sequence length="459" mass="46985" checksum="264D3C4AB6EF4704" modified="2009-07-28" version="1">MVQVNKILTGIVGGAGLALAADSCNPLKSSGCSADAALAGSFSEDFTSEPAHFKKTEYATGIIDYSDSGMNMTIAKRYDNPSLKSDFYIMYGKVEVELKAAPGQGIVSSFYLQSDDLDEIDLEWVGGDTTQFQTNYFSKGNTATYDRGAFHSAASPQETFHNYTIDWAMDKTVFYLDGVAVRTLTNDSSQGYPESPMYVMMGSWAGGDSDNAPGTIEWAGGLTDYTQAPFSMFIKRVIVSDYSSGEEYSYNGQSGSWESIEAKNGEVYGRYDQAQSEFAALAGGESISSSATVHSSSTSSASRSASSTASSTSNSSSTVSSTSSTSSSASTTLSSSSKAGYNSTSSAAPSSTSSLSSVSSKASTSSSAASTKASASSSVSSSAASSKASASASASSTAAAASSKVSAATTTSASSTLATSTAEAASSSTSEYLAIATTNGAFALQSKLSSFLAALVFLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-28" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>C5DCI2</accession>
    <name>C5DCI2_LACTC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">ATP-dependent RNA helicase DRS1</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">ATP-dependent RNA helicase drs1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="ordered locus">KLTH0B03278g</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)</name>
        <name type="common">Yeast</name>
        <name type="synonym">Kluyveromyces thermotolerans</name>
        <dbReference type="NCBI Taxonomy" id="559295"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Saccharomycetes</taxon>
            <taxon>Saccharomycetales</taxon>
            <taxon>Saccharomycetaceae</taxon>
            <taxon>Lachancea</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2009" name="Genome Res." volume="19" first="1696" last="1709">
            <title>Comparative genomics of protoploid Saccharomycetaceae.</title>
            <authorList>
                <consortium name="The Genolevures Consortium"/>
                <person name="Souciet J.-L."/>
                <person name="Dujon B."/>
                <person name="Gaillardin C."/>
                <person name="Johnston M."/>
                <person name="Baret P.V."/>
                <person name="Cliften P."/>
                <person name="Sherman D.J."/>
                <person name="Weissenbach J."/>
                <person name="Westhof E."/>
                <person name="Wincker P."/>
                <person name="Jubin C."/>
                <person name="Poulain J."/>
                <person name="Barbe V."/>
                <person name="Segurens B."/>
                <person name="Artiguenave F."/>
                <person name="Anthouard V."/>
                <person name="Vacherie B."/>
                <person name="Val M.-E."/>
                <person name="Fulton R.S."/>
                <person name="Minx P."/>
                <person name="Wilson R."/>
                <person name="Durrens P."/>
                <person name="Jean G."/>
                <person name="Marck C."/>
                <person name="Martin T."/>
                <person name="Nikolski M."/>
                <person name="Rolland T."/>
                <person name="Seret M.-L."/>
                <person name="Casaregola S."/>
                <person name="Despons L."/>
                <person name="Fairhead C."/>
                <person name="Fischer G."/>
                <person name="Lafontaine I."/>
                <person name="Leh V."/>
                <person name="Lemaire M."/>
                <person name="de Montigny J."/>
                <person name="Neuveglise C."/>
                <person name="Thierry A."/>
                <person name="Blanc-Lenfle I."/>
                <person name="Bleykasten C."/>
                <person name="Diffels J."/>
                <person name="Fritsch E."/>
                <person name="Frangeul L."/>
                <person name="Goeffon A."/>
                <person name="Jauniaux N."/>
                <person name="Kachouri-Lafond R."/>
                <person name="Payen C."/>
                <person name="Potier S."/>
                <person name="Pribylova L."/>
                <person name="Ozanne C."/>
                <person name="Richard G.-F."/>
                <person name="Sacerdot C."/>
                <person name="Straub M.-L."/>
                <person name="Talla E."/>
            </authorList>
            <dbReference type="PubMed" id="19525356"/>
            <dbReference type="DOI" id="10.1101/gr.091546.109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">ATCC 56472 / CBS 6340 / NRRL Y-8284</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">ATP-binding RNA helicase involved in ribosome assembly.</text>
    </comment>
    <comment type="catalytic activit
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